LOCUS       DACDMM010000001        67397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDMM010000001 DACDMM010000000
VERSION     DACDMM010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.001"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..970)
                     /locus_tag="IWD16_000001"
     CDS             complement(<1..970)
                     /locus_tag="IWD16_000001"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAP9087090.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESF"
     gene            complement(1247..3049)
                     /locus_tag="IWD16_000002"
     CDS             complement(1247..3049)
                     /locus_tag="IWD16_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9087091.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(3063..3815)
                     /locus_tag="IWD16_000003"
     CDS             complement(3063..3815)
                     /locus_tag="IWD16_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9087092.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(3824..4186)
                     /locus_tag="IWD16_000004"
     CDS             complement(3824..4186)
                     /locus_tag="IWD16_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9087093.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(4362..5234)
                     /locus_tag="IWD16_000005"
     CDS             complement(4362..5234)
                     /locus_tag="IWD16_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP9087094.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(5260..5772)
                     /locus_tag="IWD16_000006"
     CDS             complement(5260..5772)
                     /locus_tag="IWD16_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9087095.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(5910..6740)
                     /locus_tag="IWD16_000007"
     CDS             complement(5910..6740)
                     /locus_tag="IWD16_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP9087096.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(6756..7364)
                     /locus_tag="IWD16_000008"
     CDS             complement(6756..7364)
                     /locus_tag="IWD16_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP9087097.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(7357..7674)
                     /locus_tag="IWD16_000009"
     CDS             complement(7357..7674)
                     /locus_tag="IWD16_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9087098.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(7830..8558)
                     /locus_tag="IWD16_000010"
     CDS             complement(7830..8558)
                     /locus_tag="IWD16_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP9087099.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            8717..9598
                     /locus_tag="IWD16_000011"
     CDS             8717..9598
                     /locus_tag="IWD16_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9087100.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(9835..10878)
                     /locus_tag="IWD16_000012"
     CDS             complement(9835..10878)
                     /locus_tag="IWD16_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9087101.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(10881..11549)
                     /locus_tag="IWD16_000013"
     CDS             complement(10881..11549)
                     /locus_tag="IWD16_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9087102.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11691..14027
                     /locus_tag="IWD16_000014"
     CDS             11691..14027
                     /locus_tag="IWD16_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9087103.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(14142..14453)
                     /locus_tag="IWD16_000015"
     CDS             complement(14142..14453)
                     /locus_tag="IWD16_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087104.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            14634..16109
                     /locus_tag="IWD16_000016"
     CDS             14634..16109
                     /locus_tag="IWD16_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP9087105.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            16156..16740
                     /locus_tag="IWD16_000017"
     CDS             16156..16740
                     /locus_tag="IWD16_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP9087106.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            16742..17161
                     /locus_tag="IWD16_000018"
     CDS             16742..17161
                     /locus_tag="IWD16_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP9087107.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(17442..17726)
                     /locus_tag="IWD16_000019"
     CDS             complement(17442..17726)
                     /locus_tag="IWD16_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP9087108.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(18078..18788)
                     /locus_tag="IWD16_000020"
     CDS             complement(18078..18788)
                     /locus_tag="IWD16_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP9087109.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(18785..20257)
                     /locus_tag="IWD16_000021"
     CDS             complement(18785..20257)
                     /locus_tag="IWD16_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9087110.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            20395..20868
                     /locus_tag="IWD16_000022"
     CDS             20395..20868
                     /locus_tag="IWD16_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9087111.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(21021..21572)
                     /locus_tag="IWD16_000023"
     CDS             complement(21021..21572)
                     /locus_tag="IWD16_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9087112.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            21677..22207
                     /locus_tag="IWD16_000024"
     CDS             21677..22207
                     /locus_tag="IWD16_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP9087113.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(22690..23133)
                     /locus_tag="IWD16_000025"
     CDS             complement(22690..23133)
                     /locus_tag="IWD16_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9087114.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(23149..24309)
                     /locus_tag="IWD16_000026"
     CDS             complement(23149..24309)
                     /locus_tag="IWD16_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9087115.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            24816..26516
                     /locus_tag="IWD16_000027"
     CDS             24816..26516
                     /locus_tag="IWD16_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087116.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(26618..27451)
                     /locus_tag="IWD16_000028"
     CDS             complement(26618..27451)
                     /locus_tag="IWD16_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP9087117.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(27755..29920)
                     /locus_tag="IWD16_000029"
     CDS             complement(27755..29920)
                     /locus_tag="IWD16_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9087118.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            30180..32474
                     /locus_tag="IWD16_000030"
     CDS             30180..32474
                     /locus_tag="IWD16_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9087119.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            32467..33132
                     /gene="pgmB"
                     /locus_tag="IWD16_000031"
     CDS             32467..33132
                     /gene="pgmB"
                     /locus_tag="IWD16_000031"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9087120.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            33125..34132
                     /locus_tag="IWD16_000032"
     CDS             33125..34132
                     /locus_tag="IWD16_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9087121.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            34154..35173
                     /locus_tag="IWD16_000033"
     CDS             34154..35173
                     /locus_tag="IWD16_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9087122.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(35454..36821)
                     /gene="nhaC"
                     /locus_tag="IWD16_000034"
     CDS             complement(35454..36821)
                     /gene="nhaC"
                     /locus_tag="IWD16_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9087123.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(36931..38352)
                     /locus_tag="IWD16_000035"
     CDS             complement(36931..38352)
                     /locus_tag="IWD16_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9087124.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(38595..40472)
                     /gene="tdc"
                     /locus_tag="IWD16_000036"
     CDS             complement(38595..40472)
                     /gene="tdc"
                     /locus_tag="IWD16_000036"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9087125.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(40763..42019)
                     /locus_tag="IWD16_000037"
     CDS             complement(40763..42019)
                     /locus_tag="IWD16_000037"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP9087126.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            42599..43900
                     /locus_tag="IWD16_000038"
     CDS             42599..43900
                     /locus_tag="IWD16_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9087127.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(43969..44568)
                     /locus_tag="IWD16_000039"
     CDS             complement(43969..44568)
                     /locus_tag="IWD16_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9087128.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            44822..45703
                     /locus_tag="IWD16_000040"
     CDS             44822..45703
                     /locus_tag="IWD16_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9087129.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            45716..47326
                     /locus_tag="IWD16_000041"
     CDS             45716..47326
                     /locus_tag="IWD16_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP9087130.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(47403..48062)
                     /locus_tag="IWD16_000042"
     CDS             complement(47403..48062)
                     /locus_tag="IWD16_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9087131.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(48345..49010)
                     /locus_tag="IWD16_000043"
     CDS             complement(48345..49010)
                     /locus_tag="IWD16_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087132.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(49157..49762)
                     /gene="dhaL"
                     /locus_tag="IWD16_000044"
     CDS             complement(49157..49762)
                     /gene="dhaL"
                     /locus_tag="IWD16_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP9087133.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(49790..50773)
                     /gene="dhaK"
                     /locus_tag="IWD16_000045"
     CDS             complement(49790..50773)
                     /gene="dhaK"
                     /locus_tag="IWD16_000045"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP9087134.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(50775..51170)
                     /gene="dhaM"
                     /locus_tag="IWD16_000046"
     CDS             complement(50775..51170)
                     /gene="dhaM"
                     /locus_tag="IWD16_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP9087135.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            51324..51644
                     /locus_tag="IWD16_000047"
     CDS             51324..51644
                     /locus_tag="IWD16_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP9087136.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(51756..52625)
                     /locus_tag="IWD16_000048"
     CDS             complement(51756..52625)
                     /locus_tag="IWD16_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9087137.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(52721..53995)
                     /locus_tag="IWD16_000049"
     CDS             complement(52721..53995)
                     /locus_tag="IWD16_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9087138.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(54021..54530)
                     /locus_tag="IWD16_000050"
     CDS             complement(54021..54530)
                     /locus_tag="IWD16_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087139.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(54705..56840)
                     /locus_tag="IWD16_000051"
     CDS             complement(54705..56840)
                     /locus_tag="IWD16_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9087140.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(56923..58578)
                     /locus_tag="IWD16_000052"
     CDS             complement(56923..58578)
                     /locus_tag="IWD16_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9087141.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(58580..58894)
                     /locus_tag="IWD16_000053"
     CDS             complement(58580..58894)
                     /locus_tag="IWD16_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9087142.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            59064..59324
                     /locus_tag="IWD16_000054"
     CDS             59064..59324
                     /locus_tag="IWD16_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP9087143.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            59338..59652
                     /locus_tag="IWD16_000055"
     CDS             59338..59652
                     /locus_tag="IWD16_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP9087144.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(60382..60936)
                     /locus_tag="IWD16_000056"
     CDS             complement(60382..60936)
                     /locus_tag="IWD16_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP9087145.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(61062..62990)
                     /locus_tag="IWD16_000057"
     CDS             complement(61062..62990)
                     /locus_tag="IWD16_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9087146.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(63296..64012)
                     /locus_tag="IWD16_000058"
     CDS             complement(63296..64012)
                     /locus_tag="IWD16_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9087147.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(64013..65836)
                     /gene="glpO"
                     /locus_tag="IWD16_000059"
     CDS             complement(64013..65836)
                     /gene="glpO"
                     /locus_tag="IWD16_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP9087148.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(65850..67346)
                     /gene="glpK"
                     /locus_tag="IWD16_000060"
     CDS             complement(65850..67346)
                     /gene="glpK"
                     /locus_tag="IWD16_000060"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP9087149.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
BASE COUNT        19824 a        14073 c        11975 g        21525 t
ORIGIN      
        1 caaaactttc aagtcgctcc gctcctggca gttcttgagt ctcaaatgtg ataccgccat
       61 gatttactaa tttattcccg cgcatttccg gtccatcttc tccaaaattt gctgtaaaaa
      121 tcacgattcc tgaagcatct gtttttactt ggatcgatac tttttcatct gggcttgtca
      181 attccgcagc atttagtaaa ccagattgtt ttttcaacaa aaatggatga tcgatgccgc
      241 ccactatcat tgattggcga aaatcactgg caaagacctc acctaatttt ttaggacgtt
      301 ggaagtcaaa tgctgtgcca cttactgaaa ctacttctcc tgtaggcgtt gtatctttat
      361 tgagcgtagc aaataatcgg ctattcaacc aaagtatatg ttcgtcgatt gactgtgctg
      421 gatcaccggt taaattgaaa tagacatgat ttgtaggatt gaatactgtt ttgcggtcac
      481 tgatcccttg gaccgtcact cgccatgtat catcgttcgt cagcgtatac cgtacttcta
      541 catctaaagt tccaggaaat ccgtttccca tatctgggct agtctgatag aaaatgaccg
      601 atgcttcgtt ttcaccattg atgactgtat aatgccactg cttggcttca aagctaggtg
      661 caccaccatg taaacaatga ccagtgtccg ggtctgtttt tacttcgaac gtttcatcat
      721 ctaatttgaa tgtgccattc gtaatccgtc cagccatacg tccgattgaa gcaccgatat
      781 aagggtcttt ttctagatat tcttttgctt catcaaaacc taagatcaac tctcgttttt
      841 cattatcgat tattgtttga aaatgaacaa cccgtgcccc aagatcactg acagacaaag
      901 atgtcccatt ttgattcgtg atcgtaatca agtgatagtt ttcgccaaaa ttttgttcat
      961 tgatttccat gttccttgca ccctcttttc tttcatttgc actgattcca gtatacatgg
     1021 aaaccgtttt cttaacagct tattccgtga gtaacgagaa aaaatttact atattctaat
     1081 aaaagaccgt gaaaaaaagg ttgtgacaaa aatattgtca caacctcttt ttccgaacaa
     1141 acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atatgaggag
     1201 ctatttttgc ttggctgttc ttattacttg atgcaggaca gctttttcac aatctctttt
     1261 tcaatacgat tccagtatat aaccatgtcg tcagtacata gtagatgaaa tacaatacga
     1321 agaagatcgt aaacggaacc caaatatctt tgtatggatt ttgcatgaat agaccaaaca
     1381 tttgtaatcc gaataagaca tggactacac ctaagattcc tggtgctaag aataacacgc
     1441 ctacttctct acggatcgat tgtttaagca acccgcgtcg tgttcctatc ttttcaagca
     1501 tttcataacg agcaatgtca ctcttgcttc ctgacaagat cttgaacatc aaacagctag
     1561 caagcatggt caagaaagct aaacctagga agaatcccat aaattcaaaa ccagagaata
     1621 gaccattata cacttcatac atttcatatt tctggttgct gtactctact ttttgcaaag
     1681 atggattgtt cattttattt tgagcaacaa gtgtttcgat tggtttcagg tttgcttgga
     1741 agtcttttac ctgaactagc tgtaaagtgg attgtggcaa gttcagttga tcaaattctg
     1801 attgactgac taccttgatt tctttttctt gctgttccgg caattcatag cttcgaagtt
     1861 cctcttgtac agtcagttca ttcatttttt tagcaggaac tttttctttc gtgatcttcg
     1921 gcggtgttcc atcttcatgt tttatcatga tcaatggttg agccgcaaat tcacttgcat
     1981 tatagtaaat cgttcccgca tcttcttttt gagtataaga agcagaaagt gttggtgaaa
     2041 gttctttgat tttttgctga tcttctgccc gaggattatt catcactaga tcatatccag
     2101 aattgttttc tgtcaagtct gcaatttcat ttttaaaacc taatcctacc gtcaaagcgc
     2161 ccagtgccaa ggcaaacagc atagcgacca ttgagagcat ttgtgtatat tcacgaatac
     2221 gaaagtttaa ctgtgacaat gtgaaattgt tcaatttttt caatgaaatc tgttctgatt
     2281 tctttagtaa tgataaaaag aaaatgatca cagaatggaa aatgaaataa gttcctaata
     2341 tgatcgtaac taaggctacc cctataccaa agacttgata ggtccccacg tgtttcatca
     2401 tatagtaacc gcagcctaga aaagcaattc ccagtactac ttcaaggaaa aacaagaatt
     2461 tatgctgctt gattcttgtt ggtgtctgat ccgcacgaat caatgctaaa atcggttttt
     2521 tgacaatcga aaaagcattg acaacagcag ctaataaaaa caaaacagca aaaaacaaaa
     2581 cagtgatcaa caacccgtga atactaaaag gtgtaaaatg cgaaatattg agatctaact
     2641 gatccactaa taatcggtgg acgatacttg tcaatcctac accgacagct gaacccacta
     2701 aagtagctgc gatccctact aagaatgttt cagaaaaaat catctgtgcg atttttctgc
     2761 cttttgctcc cagcatcata aacattgcat agtctttttg ccgcatcgtc atcaaaaagg
     2821 aatttgcata taaaatataa acgaatgtga tgatccctag taaaacagaa ccgaatcgga
     2881 aaatgataac tgtagccgca acgatcgtat tacttttcaa aaaagcttcg ttgcttgcca
     2941 ttgattcaaa catgtaaaaa atagcagatg ccataactaa tcctgagaat aacaccaagt
     3001 agtctcttaa acggctcttg atcccagtaa gtgataattt ccaaagcata gtcaatctcc
     3061 ccctactgtt ccaagtttcc aagaatagat agtatttctc gatagaagga atcacggctt
     3121 tgatctccac gtttcacttc ttgatggata ccgccatcct tgatgaataa aatacgttgg
     3181 caataacttg cagaaaacgg atcatgcgtc acaagaagaa tcgatacatg atcttttgta
     3241 ttcaattcgt ccatcgtatc taaaagatcc cgggcacttt tggaatccag cgcacctgta
     3301 ggctcatccc ctaatagaat cgttggttgt gtgatcaatg cacgtgcggc ggcgacccgt
     3361 tgtttttgtc caccggaaat ctcagaagga tagctttcca aaatatgaga gatggataaa
     3421 cgttctgcaa ttttttggac acgttgcttg atttcttttg gtctgacgcc ttgtaaagaa
     3481 agaggaacag cgatattttc ttgtgctgtc aagctttcta ataaattgaa atcttggaaa
     3541 ataaagccaa tctcattcgc acgaaaatct gctagttgat ttccttttaa tgtggtcaca
     3601 tctttttgat tgattcggat atgcccatct gttggtttat ctaatgtgga taaaatattc
     3661 aaaagtgtcg acttaccaga accagaagcg cccatgatcc caataaattc gcctttttct
     3721 acatcgaagc tgatgccgct taatgcttgc gtctttttct cattgttttt tccgtatact
     3781 tttgttacat tctgtacttc gactattttg ttcatttctt tccctaccct ttaattagtt
     3841 tttccagtgc tgttttcggc acgtcttttt tttcgacttc ttcatacgaa gtgacacctt
     3901 tatttttgtt ccaggtgatt tttaaatatg ctcctttacg aagcggtcga gtcaattgtc
     3961 ccttgaagtc cagttctttt tccttaccct ctttcgtata gccaggcagg atatatctat
     4021 agttgactgt ttcttgtcca gagtctgatt ttgaaactgt ttttgtacca tctgtcgtaa
     4081 tttgtacata gtattcttct ccacccataa ctacgtaatc tgcgattttc acccctgcaa
     4141 gtaccacaac gatgaaaaca agtagaccga tcagaagttt tttcactttt tccccttctc
     4201 tcttacttta tatactgatt atagaaaaaa gcgaacaatt ctgccttcga atcaactatc
     4261 attcttcttt caattttgtc attttcttta aaacaactaa aaaaagagtt caagacaata
     4321 atccgtccta aactccttct tcctatctta caaaaagtat gtcacttacg gtctttcatg
     4381 tcgcctgtat tgataacagg ttgcgttcct ttgtctgtaa tgattgagac aagtaaatta
     4441 ttgatcaatt ggactttccg atcatcggtg aaattgattt cttgtccatc ttcgatttgc
     4501 tctaaggcca tttgagtgat tgtgacagct ccttcaacaa tggtttgtcg agcagacaaa
     4561 atggcttttg cttgttgacg ttggagcatg gcactagcga tttctgttgc ataggctaaa
     4621 tggttcaagc gtgtttcgat tacttcgaca cctgctactg ctaaacgctc ttgcagctct
     4681 ttttgcaact cgtcagacac tgccgtcgtg tttcctcgaa gcgtcaaatc attctcattg
     4741 aatgtatcat acgggtattg gctagcgata tgccgaatgg ctgtctcact ttggatttct
     4801 acaaaatctt gataatacgc tacatcgaat aatgcctttg ctgtatcaac gactttaaag
     4861 actactactg ctgagatttc gatggggttt ccatccaaat cattgacttt taaaacagaa
     4921 ctattgaaat tccgaacttt caacgaaaca gtcatttttt gtgccaaagg tatcgtaagg
     4981 aaaaagccat tttctcgaat cgtacctaaa tattgtccaa aaaacaaaat tacttttgct
     5041 tgattaggac tgactacagt tgccgaactt aacaacaata acgtgatcaa ccaaagaaaa
     5101 atacttaaaa aaagtgccca aactttcgct tgccacatac ctaaatagaa gaaaaacaat
     5161 ccaactaaag ctaatacgac caatccgaca atccctgcat aaccattgat ataaaatgct
     5221 cttttttctc tcatgctact tcctgcgctc ctttatcctt caataaatat ctaatgttac
     5281 ttttttaatt ttttttcgaa tacgcgccat tacaccttct agttctaact ctttgtcttc
     5341 agtgatgtac caaccccttg cctgtgtccc tccggctgcc tgcggtaaat aatccaattt
     5401 tgttcgaacc cagtgctttt cttctgtaaa atagtcttct accaagacct ctagatccat
     5461 ccctgctgaa agaggcaaat cttcccgaac agatttaaag ataaatcgtt ccttcttccc
     5521 atcatacttc aatattcctg tatcgataat cggtaaagaa taataatcca gctctttctt
     5581 caccgtcaat aaatgtttga aaacttcatg gtacacttgc tctaattggc gaccttctga
     5641 attgttcggg taattaacca tcactttttc tttgtctgga tcatagaagg tttggcttag
     5701 ttgagtgata taagtatgta atctttccat ttctgttttt aacttttctc tttgaccttc
     5761 ttctaccttc atatactctt tcaccctgtt ttctttttag atattttctt tcttatttta
     5821 ctatagttat gtgcgttaag cgatgaaaaa cagcaaagat tggaaggatc tttgctgttt
     5881 tttcttaaaa tatccagttg agtacaggat caatctgttt cattgtcctc ttgatccgtc
     5941 gtaatattga tcgatgtgtc tttagcagtg atctttagct ttttctgtcc ttgttctaac
     6001 aaagtatttg ttattggctg gtagtctttt tcaatataag attcaccaga cgtttggata
     6061 gtatagccac tgttttccat ctcatgcaaa gataaattgc caccgtttag gatgactttt
     6121 ccttctttgc tgatcaaact gtcttctaga tggaagttgc tgttttcacc tgtgacgatc
     6181 aagttatcca cattggcctc atctaatcga agtgagccta attcatcttt cgcagttatt
     6241 gttcctcctg taaattcatt gaacgatacc cagccatcat gtgtcttagt attcactttt
     6301 ttgattgata cttcgttgat atcaattgca ctattattcc cttcgattgt caattcagat
     6361 aattcatgat ctgcagggac aacaatagtg atttgtgcac ttgtgtcttc taaactaatg
     6421 attgcacctg ttgatttttt gactgcattc acagtcaaca cgccattttt ctcctttatt
     6481 tctggctttt ctgtcttatc ataagtcgtt tttatctcaa aatccaatcc gcgtatgacc
     6541 gaaactgttt gattttctgc atggatgacg attttgtttg catccttgat tttttttgtc
     6601 tcagtaacca tatcgatcaa ccgaggacca ttatcccaag tcagactctt ttgagcgcct
     6661 aatgtataag ttgcacctgc taggaggcca ccagtaagca tcagaccaat accggtaatc
     6721 aaaaaatgtt tcaatctcaa tttatgtgtc ctccttcaaa cgcccgctga atgcagatct
     6781 ctttttgtaa taaaacgtcg tcccatccat ttaacaaagt tcatcatgac ataaaaaagc
     6841 ccttttgttc ctttcagcaa cagtggcgaa ataaatactg cgcttcctgt ggcgaacaga
     6901 ccgacaccga taaagaataa tccactcaga atcgattgtc caaacacact gattcctgag
     6961 atgatcatga ataggccaac acctaagata ctgatacata aaacatagat actaaaaatg
     7021 agcgctagta aagcaaccaa tagtccccca ataaccaata taaaccccag tgctagtgga
     7081 atgaggacag gagaagcaaa taaaccgata atgattaacc aaatcaggcg aatttgtctt
     7141 tttggatttt tcactaactc gttttctgct tgttcttctt ctgaaaagta actgattttt
     7201 aattttaagg caaactgctt tggtgtacca tattcatcaa tgatctgatc taatgttttt
     7261 ccagaatcta aaaaatattc ctcataatac tggatcaaat caaaacgatc gtcttcagga
     7321 atttcttgaa gatgatccac aacttcattc aaatattcat tcatttgtgc tctcctccaa
     7381 taaccggtct aaagaatctc gaaaaacaat ccattcatca ataatttctt ctaaatgacg
     7441 acttcccatt ggtgtcaacg agtaatatcg acggtttctg ccttcaaaag gttcatcata
     7501 ggtctcaaga tatccttctt ttttcaatct tctcaaaacc ggatatagtg tagattcact
     7561 gacagagaga ctttcttgta cgatttttgt taaggcatac ccatacatat cttccttgct
     7621 taatgctgct agcacagtac catcaagaag aacagttgga atctggatgt gcattacgtc
     7681 accttctatt ctcaagtgta atattttatt tcaatctata ttatacaaca tataatatag
     7741 tttcaaaatc agtttgtcaa atacaaaaaa actgactaaa agatttgatc ctttttagtc
     7801 agcaaaaaat ggcttctctt tgatttttat tataacagtg gcataatcca atttgtccac
     7861 atccagctca gtggcatgat aacgatcatt gcaggtatca tgtcagctac tgggaacatt
     7921 ttcacgttga tcattctaaa accggtagcg accatcaaga atccgccgca agctttaaaa
     7981 tctaagatca tgtctggtgt agtcaatgga taaataaatt ttgccaataa gaacaaaata
     8041 taaaaaataa tgaattgggg aattgctatc acagaaacga cataccctaa attacacgca
     8101 aaaatagctg ctgtgaaaaa atcaaggaca gatttagaaa tcaaaatcgt actgtctcct
     8161 gtcatcccag aatctaaact tccatagatc cccgtaccac ttgcacaaaa tagaacaatg
     8221 accgtcacta atgtgctgat aaattcatcg tgggtcatac caagcttttc actagggaag
     8281 attttagaaa taggtacttg catcagcgca gcccccttat ttatcccatt acctaaatga
     8341 acaatcagac caatcgctgt tcctatcact aaagcaaaaa taacagccgg catatttttc
     8401 atcggcgcaa tggaatagat tcccatcccc atcgaacaca caccaaaaac catcgtcagc
     8461 tgtgctttaa aatgttcact taatttattt cctactaatc cgccaattag tccgccaagc
     8521 aaaacggaac ctacattgat caaaactcca gttggcatca aaaaactcct ctctttttct
     8581 acgaagcgaa aaaaatcaga aaaatttctg taattattgc tttcgctgca agaaagattg
     8641 taacagacag agaagattac accaattcat tttttgtgtt aaagtattct ttattggaat
     8701 aagtaggagg aatatgatgg atatccataa attcgaagtt tttcttgatt tagccgaaac
     8761 aatgaatttc accaaaactg ctgatcgaca attcaccacc caaggaaata tttctaaaca
     8821 gattctttcg ttagaaacag aattagacgt caaattattc gaacgcgccc atagaaaaat
     8881 cgaattgacc gaagctggaa gcttattgct cccatatgta aaaaatgtcg tagaacaata
     8941 ccatgctatg caagaagtgc ttgaaaccta tacgaaagac aacaatcttt cattaaatat
     9001 cttaacgata ccgacaatga tcaattataa aggtttttcc aaaatcaccg agtttttgaa
     9061 acgccatcct gaatttacag tccagttgaa agaagtggaa agtgtcgaat taacttacaa
     9121 tgacgccaat gaacatagtg acacgatcca ctttgctaga aatttccagg ccccaccaag
     9181 ttcgattgag tggttgccta ctgaaacaga cgattttgta gcagttctac ctaaaaatca
     9241 tcctttagct agtaaaaaga aattggatct aagcgaactg aaaaaggaga actttttgct
     9301 ccttggcccc actacgaact tgtatcaacc tgtactagat ctgtgtcgcg aagctggatt
     9361 tgaaccaaaa atctcttata aaggagcaag aatcgacttg atcatcaata tgattgcaaa
     9421 tgacatgggg attgctattg ttatggaaaa aacagtcaag aatctattaa aagaaaatac
     9481 tgtgattctc cctatttctc caacaacaga gagttatctt gcatttactc gtaaagtcgg
     9541 tgagcattct ttggcttcag atacattttg ggagtattta aatcaagaag aaaactaagc
     9601 cctacatttt ctgtccatct atccacccaa aaaaagagag cgacgcatga ctttcgctct
     9661 ctttcttgca tcatgtcctt gtttcaatga cacctctaac atttctcgta gttcaataag
     9721 aaagcagcga tttttatgcc aaaagaaccg taagaacatt tccctaatca caaaaaaacg
     9781 actaaaaacc cctaaaaacc ttcaatagaa tttttagggg ttttattctt tttcctattg
     9841 tttacttaac cgtatcattg ctgtcgttcc tactccttcg atactgctat aatcaatacg
     9901 tccatcatgc agttctagaa tttttttaac cagtgaaagt cctagcccat tcccagaaat
     9961 ttgacgactt gtatctcctt gataaaattt ttcaaacatt cgatctctgg tctcttcatt
    10021 cattcctaca cctgaatcgg tcacttccag tccaatctct gtggctgtct caaatagttt
    10081 gatatgaatc tgtccattga tttgattgta ctttatggcg ttatccatga tattcagcca
    10141 cacttggtac aaaaactctt cattgcctgt gtaatttact gcagcaagtt cgatatccaa
    10201 ttctagctgt tctttctccc atttaggctg caaaaaaacg attacttctc tgatttgttc
    10261 atctaatcga tactctttct tttccagttg gatacgctgg ttttccagtt tgttcaattt
    10321 taagatgttc tctgttaatt gtgaaagctg cgtgatactt tcgataatcc gatataaaaa
    10381 tatttggcgt tcttcgtcag agagatttgg ggcttgtaac aattgaacat atccttggat
    10441 tgttgctagt ggtgttttga attcatggga tacactggac acaaagtcat tcctcagtgt
    10501 ctcgatacta ttgagttctt ggaccataac gttgaaatct ttatacagtt gctgtacttc
    10561 tgctactttc tgctgttcat ccattcgaat cgaaaaatct cccgttgcta ccagactcat
    10621 atttgtccct agcgcactga ttggatgcag gatacgccgc ccaacaaaaa tcgatattcc
    10681 tgtacccacg accatactga accccgcaaa gacgaataac gggtataacg ttggttttcc
    10741 agccgattca gcaggcgaga tagaatgtcg agacaataag aaaataaaac cgagaaaaca
    10801 cagcagtgtc actaagatcg aaatgaagac aatactagca aaatagatcc ataattgtga
    10861 aaatagcttt tttttcatct ttaaataacc gccttatagc ctagtccccg tactgtgatg
    10921 atttgaaaat cttccgtctt ttcaaaacgg gtccttaaac gtttgatatg tacatcgatc
    10981 gttcgttctt ctgtattcgt atctaatccc caaatatcat ccatcaactg ttggcgagtg
    11041 aagatcttgt tgggataaga cagtaattta tacagaagca gaaattcttt ttgaggcaga
    11101 atcaacgaat gctgatcttg taacactgta taagcatcta gctctaattt tgtttgtccg
    11161 attatcaagc tactgctgta attgatcttt gaacgtcgaa gcaatgcttc cacacgtagg
    11221 atcatctcat tgacatcgat gggttttgtc atataatcat ctgctcctaa tttgaacccg
    11281 cgtttcttat cctcaaaatc ggcctttgcc gtaatcatta gaacaggtgt gtcaaatctg
    11341 gtgctacgta atgagtctag caagtcatag ccatccattt tcggcatcat gatatctgta
    11401 atgattaatt ctatacgtgt cgcttctagt tttttcagcg catcctctcc attcaatgcg
    11461 gtgatgactg aaaagcccgc tcgttccaac acagaacggt aaagcaactg taattgcgga
    11521 tcatcttcta ctactaaaat acgattcatt gatctccctc tgttttttga aaaatttcta
    11581 gattttttct tgtagttcat cttcagttaa catttatttc gtatactacg atcattgttc
    11641 tgatatactt attagtatac cgaatatttc aatacgggag aagggtgatt atgcttcaat
    11701 taaaagatat aaaaaaatat tataaagtcg gcgaaaccac caccaaagca ttggatggtg
    11761 tgtctgtcgc tttcagacaa aaagaattcg tagcgatttt aggtcctagt ggttctggta
    11821 aaacgactat gctgaatgtg attggtggac tagataatta tgattccggt gatatggtaa
    11881 tcaacggaaa gtctactaaa gactttaagg acagtgactg ggatgcctac cgaaacaact
    11941 ctatcggttt catttttcaa agttacaact tgattggtca tctaggcatt attgaaaacg
    12001 ttgaacttgg gatgacatta agtggtgttt ctaaagacga aaaacgaaaa cgggcagaag
    12061 atgcattgca ccgagtcggc ttgacagatc atatgcataa aaagccaaat cagttatctg
    12121 gtggacaaat gcagcgagtg gccatcgctc gagcactagc caatgaccca gatatccttt
    12181 tgtgcgatga accaaccgga gcgttagata cagaaacaag tatccagatc atggaattga
    12241 tccaagagtt atcgaaagaa aaattagtga tcatggtcac acataatcct gaactggccc
    12301 atcaatacgc agatcggatc attgaatttt ctgatggtaa gatcttaaca gattcccatc
    12361 ctcacatcga acgaccaaaa gatgatcaat ttaacttacg cagaacaaag atgagttttt
    12421 ggacagcgtt aaagctttcc ttcaacaata tccgaacaaa aaaaggacgt actttcttga
    12481 cctcctttgc ttccagtatt gggatcatcg gaatcgcgat cgttctgtcc ctttcttctg
    12541 gattccaaaa acaaatcgat aacacgcaag ctgaaacgat ggcgaaattt ccgattacta
    12601 tttctaaggt aacgactaac cagactcgtg acgatgccgg attgggtgca agcaaagcgg
    12661 attatccaga cagcaaaacg attactgcaa aagtcagcga agaagataaa gcacagcaca
    12721 cgaataagat tgatcaaaca tacgtcgatt acgtaacaga catcgatcct aacttaagta
    12781 ataatatcgg atttacccgt actacaggaa tcaatctttt acgtgatgta aatggcaaag
    12841 tacagcctgt cagcttctcg aatcaaaatc ctgatacgga atctctttct ttatctagta
    12901 cgatgtcagc aatgacaggt gtgggagtgt caagtttccc tacacagctt gacactagca
    12961 aagaaaactt tttaaaagac aattattctt tacttgccgg aagttatcca gcctcagcaa
    13021 cagatgtcgt gctgatcgta gacggcaata ataatactaa tatcaacgca ttgaagaatc
    13081 ttggatttga tgtgaaagaa gacgaaaaac ttgattttga tgatatcgtt ggtacaacat
    13141 tcaaattagt aaacaacaat acttactata caaaacttcc aactgggaat tttatcccta
    13201 atacagatta tgatgcgatg tatcagaatg cttctgatga attgaagatc tcggggatcc
    13261 tacgcgtgaa atcttcttct acaatgaatc tcttatctcc aggaatcgct tatagcgatc
    13321 aattaacgac tcaaatcgtc aacgaaaaca aagaatcaga aatcgtcaaa gctcaaagag
    13381 acagtggtgt aaatgtctta actactgaaa aagttgacga aaatgccaaa caaacattgc
    13441 ttagttattt aggtggagac agcttgcctt caagtatcat gatttatccg aacaattttg
    13501 aagacaaaga aaagatcttg gattatctag atgattacaa taaaggcaaa tcagatgaag
    13561 ataaaatcat ttatactgat ttagctggaa cgatgactga actgactggc ggattgatgg
    13621 atgctatcac ttatgtcctt attgcttttg cagggatctc tttagtaaca agtatgatca
    13681 tgatcagtat cattacttat acatctgtga ttgaacggac aaaagaaatc ggtgtactaa
    13741 aagctttagg tgcacgtaaa aaagacatca ctcgcgtatt tgacgcagaa acgtgtattt
    13801 taggtatttc ttctgggata ttaggtgtgt ttattgcatg gctagctacg ttccctatca
    13861 atagcatctt atacaacatg acggatctaa aaaatgttgc tcaactaaat cctgtccatg
    13921 cgattattct agtgatcgtc tctactatat tgacgatgct tggcggtcat cttcctgcac
    13981 gaatggcagc gaaaaaagac gccgctatcg ctttgcgtgc agaataaggt tgtgacaacc
    14041 acatttagat ttgagtcata agagcgctga aatagtagaa aacagcaaga aacgaggaag
    14101 taatatgttc cttgtttcgc gctgtttttt ttaattattt tttataattc tggtatactt
    14161 ttctttcctt tgtaccacaa ctcttctggc gtatacctgt cttttccatt tatgaatact
    14221 gttccatctg cttcaatcgt tacttcagtc ccaatccaca tattacgaga aagtgcgaga
    14281 agattttttc tttttcctct tccttttttt ccaaattgca aggcatcggt tatctcttct
    14341 gtccaatgag atttcacgta ttttacttct ccgttttcaa atgtaacttt caaaatattc
    14401 gctatatttt ccacttttac tgctttagga aatgactcct tcattttttt cactttcctt
    14461 cctccaacac taagtatgcg ctttctttta gtgataagta tatcatagtt tattctatta
    14521 tcaaaaaaac gcatccattc cattgtagaa tactttaccg aacaagttga attgttttcc
    14581 tctttttcac cttttactat agataggtaa agacaactaa ggaggaatct ctcatgacag
    14641 atcaaccgta tgatttaaga aaagtcatcg aagaactaaa aacacttcca ggtcaatatc
    14701 acgaaacaga tattgaaatc gatcctgaag cagaattatc tggtgtttac cgttatatcg
    14761 gtgctggcgg tacagtaaaa cgtccgactc aagaaggacc agctatgact ttcaacaata
    14821 tcaaaggttt ccccggcaca cgtgtgaaca tcggaacaat ggctagtcgt aaacgtgtag
    14881 gtgaaatttt gcaccatgat tatcgaacac ttggtcgttt cttgaaagac gctgtagaaa
    14941 atccaatcaa acctgtgaaa gtagacaaag cagacgctcc agcacaagaa gtcgttcatc
    15001 tagctaccga tgcagacttc gatattcgca acattttgcc tgcacctaca aatactcctg
    15061 aagatgctgg tccatacatc acaatgggtg tagtcttagg atcagatcca gaaaaaacaa
    15121 tgagcgatgt aacgatccat cgtatggttc tagaagataa agatacgatc ggtatgtaca
    15181 tcatgcctgg cggacgccac attggtcact tccaaaaaca attcgaagcc ttgaataaac
    15241 caatgcctat cactatcaat attggtttag atccagcaat tgccattggc acgactttcg
    15301 aaccacctac cactccactt ggctacaatg aactatggtg tgcaggagct ttgcgtaacg
    15361 aacctattca aatcgttgat ggtatagcag tcgatgaagt gggtattgct cgtgctgaat
    15421 tcatcattga agcagaaatc atgccaaatg aaacaatgca agaagatatc aacacgaact
    15481 caggaaaagc aatgccagaa tttcctggct atactgggga tgctaatcca gctgtacaag
    15541 tagtgaaagt caaagcagtt actcaccgaa aagacaatcc aatcatgcaa acaacgattg
    15601 gtcccagtga agaacacgta tctatggccg gtattcctac agaagcaagt attcttgcct
    15661 tggtcgataa agctattcca ggaaaagtag taaatgtcta caatcctcct gcaggtggtg
    15721 gtaaattgat gaccatcatg caaatccgta aagaaaacga agcggatgaa ggtattcagc
    15781 gtcaagcagc tattcttgct ttatcagcct tcaaagaatt gaagacagtc attttagtcg
    15841 atgaagatgt tgatattttc gacatgaacg atgtagtttg gactttcaac actcgtttcc
    15901 aaggagataa agatatcatc gtgatcccag gaatgaggaa ccatccattg gacccatcag
    15961 aattaccaga atataatcct ggctctattc gtttccgtgg gatgagtgca aaaactatct
    16021 ttgacggtac agttcctttc gatatgaaag aacattttgt acgtgcaaaa ttccaagaag
    16081 tagaagattg ggaaaaatat ttgaaataat gaatgatctg caactgtttt gacatcataa
    16141 aaaggagtgc ggaaaatgga acaattggca caacggaaga aaaaaatcgt gattggtgta
    16201 agtggcgctt caggcaccat ttatgccatc aacctagtga agaaactaaa agaatatccg
    16261 atgatagaaa cacacggaat catcagtcca tgggcaaaac agaacttaaa gctggaatcc
    16321 gacttatcac taactgaatt cgaaagcctg tttgattacc attattcaaa caaagattta
    16381 ggtgctgctg tcgccagcgg atcatttttg accgatggaa tgatcatcgt accggcaagt
    16441 atgaaaacag tcgcaggtat ctctgttggt ttaggcgaca acctgattgc acgtgctgct
    16501 gatgtaacaa tgaaggagca acgaaagtta gtcattgttc ctcgtgaaac gccattaaat
    16561 gccattcact tggaaaactt gactaaatta gcacgactag gtgtgcaaat cataccacca
    16621 attcctgctt tttataatca gcctcaaacg atccaagatc ttgttaatca ccatacaatg
    16681 aaacttttgg atcaatttgg aattgtaaat gagcattcaa aacgttggca gggtgattaa
    16741 tatggaaaaa gaaacgatag ttgaagtaac caaactaggt ttcacgatcc aagctgctgt
    16801 taaagtaatc ggtaaagact tattgatcac tttaacaggc ggagatacac cacatatcgg
    16861 aacagtcact acattttctc gtgatacaga gatgcaaact gtacgtttcc ctagccacga
    16921 tggtcggttc cataaagacg atgtcttagc cgaaaaaata gctgtgatca tcaaaactgc
    16981 cgttccggga agttgtacga tcacttccgg tatccatgtt gaccatatct ccaatgcaca
    17041 aatcaacgcc tcttttccga tgtcagaaga gttaggttac aagattttag attggttgga
    17101 aaaaatagat ttccatatca aagaaccagt ttattataaa gatggtgaaa aaacattatg
    17161 aaagaagagg ttgtgaaatc agcgttatga cctgagtatg agagaaaaaa atggaaaaat
    17221 agcttctcat attttgccgt ttttgagcta ataccgaggg acagctgatt gaacgccgtt
    17281 tatcaagagg ttgtgataaa gccgcttcgt ttcgagtagt aagaaaaatt ttgtaaacat
    17341 gggtttccac atttttgcat aaatttgact tactaccgag aaactggctt ttgaacaccg
    17401 tttatcaaga ggttgtgaca cgtcttttgt cacaacctct tttatttttg cttcactgta
    17461 actgctacaa tatcaccgat ttcttttccg atgtttttac gaatatcttt tcgtagtcca
    17521 aggatgtagc agactgaacc atctggattt ttcacgccca tattcacgat actgccttca
    17581 tacggcatat tgtcaaaagt tgcttggact ttaactcttc cagcaccgaa ttcttttctt
    17641 atatcatacg gaaaaataat atatgccccg cccttgtcga cttcggaagc atagattttt
    17701 gattcaaaag aataaatttc cctcatttta tttctcctat tttttacatc tagtatagca
    17761 tagcaaaaaa aagaaggaac tgtgacaaaa gtcttgtcgc agttcctttt ttcgaataaa
    17821 cggtgttctc ctgccaactc ctcggtcaca agtcacaata gtcaacaaac atgagaagct
    17881 gttttttgaa attgctcctt gtgactcaga gttaaacggc aggctcacaa cctcttcctt
    17941 aaacggtgtt caaaagcttg catctacggc actaagccca atcagatgaa aaatatggag
    18001 tgctattttt atctgattgt tcttactgct tgatgcagag cagctttttc acaacctcct
    18061 acatggtgtt caaaatatta ttcgcctttt gcttctttca ctaattttgc gaatgcttgt
    18121 tgttggaatt cagcagctaa ggctttcgta cgttgttctt gctctgcttt gctcattttt
    18181 ggattttcag tcattgcttt taccataccg tcttgaacat aagcttttgc ttcactttcc
    18241 atcgtacttt gctgtttctt cactaattca gctaacttgg ctgcttggtc cgtacttaat
    18301 tctacccatg tctctgggat tttgaatgta ttcactcggc tgtcatattc gtgattgtta
    18361 tcagcaatat tgaaccagaa tttatacatg tcgtttttgt attcccaata agtagttttc
    18421 gtcacttttt cagcatcgcc gtctgtcttt tctactttat ttgtttcgtt atccgtacca
    18481 gttgcttttg gtttttcttg tttttcattt gttcggtaaa gatacacttt ttcttttcca
    18541 ttccctaaag ctttatacaa aagcatatct gctccttggg aatcagcact ggaaacaatt
    18601 ttttctgttt ttgtttccgt tactttttcc attccaaagt gattggacaa attagcgacg
    18661 atcaaactta gtgaagcgac gaagattagt ccgaaaatca aagataaaaa ggtttgccat
    18721 gtttttttag cgaacacgtt cgttaaagca aaagcaagca agctgacaat caaaactcca
    18781 ataatcatgc attatccccc gctttcatta gaacaggttc ttttttcttc aataaaaagg
    18841 caataataaa gccaagaatc ccaaagatga tcgctacgat aaacgcggca tggtaacctg
    18901 acagtgtggc attgattgcc tgatctttat aagctaatgg cgtattatcc aatacagatt
    18961 tgcttgggat atttgctttt gtcacattcg tcaacacact gatcaaaata gctgtaccaa
    19021 tcgagcttgc gatttgacgg aatgtattat ttaccgctgt cccatgactg atcaagttat
    19081 ttggcaatgc attcatacca gaagtcgtta ccggcatcat gaccatggag atcccaaaca
    19141 tacggatcgc ataaaggatc acgatatata aaactggagt cgtttttgtc aagaaagcaa
    19201 atggtgcagt tgccgctgtc aaaatgaaca tccctgtgat ggctaaacgt ttcgcgccgt
    19261 atttatcgaa aatcgcaccc gtcacaggca tcatagcccc catgatcaat gcacccggca
    19321 acaacatcaa gccagaatgg aatgctgatt ctccacgaat attttgaata tacaatggta
    19381 cgaccatttc tgcccctacc atcgccatat ttgttacacc agctaaaaca gctgcaatcg
    19441 tgaatgtaga tgatttgaat acacgaagtt ccaagaatgg atgttccaaa tgtaattgac
    19501 gccatgagaa caaggcgatg aaaatcacac caacaactaa gaaaccaatt actttcaagc
    19561 ttccccagcc gtcatctcct acactagaaa atccgtaaag taaactaccg aatccaatcg
    19621 ttgataaaat agcggataaa atatcaatgc taggatttga caatggaata acacttttca
    19681 ttaggaagaa tgctaaaacc aagacaagaa caacgattgg cagtaccata ccaaacaagt
    19741 cacgccaatg gtagttatca atgatataac cagataatgt agggcctaaa gcaggagcaa
    19801 gtccaataac gatccctgac atccccattg ctgatcctct tttttctggc gggaaaatag
    19861 atagcatgat attttgcaat aatggcatcg atactcccac gccgcaagct tggatcaagc
    19921 gaccgattag taatgtacta aaattaggtg ctgtaaagca agtaatcgtt ccgatcaaaa
    19981 agatcgtcat ggcagaaaga tataaatttc ttgaactgaa tttattgatc agccatgctg
    20041 ttattgggat catgatcccg ttgactagca agaaaccagt cgttaaccat tgcacatcag
    20101 acgctgaaat atcgaaagct ttcatcaatg taggaaaagc tgtcgtcaat aaggtctgat
    20161 tcaaaacggt acaaaatgta ccaattaata agacaacaac taaaagtgtc cgattatatg
    20221 gttttccgta aatatctacg ggttggttgt tactcatatt tctccttctt tctgaatttt
    20281 ttctgaaact actagaatac tttagtccta aattcgctat ttgtcaacct acttgacaca
    20341 taagttaata cggatataat taagggaagc aagaggacaa aaggagtgac aaaaatggtt
    20401 ggggcttccc ttaaacgttt gttggaaaat gatcaattgg ttgttggcat cagtgaatta
    20461 agtgatatcg taggtgtgtc tccacgtcaa ttaaggtatt gggaacaaaa gggattcatt
    20521 cgttcgatcg aagctgatgc gaattgtcct agaaaatatc gtttacctac agtcataaaa
    20581 gtagaactga tcaaaaaata tttagatgac ggatttactt tgtcaaaagc tgcagaaaag
    20641 gcagaaatcc gccaaaagaa aatgcaccat gtccgtaaag ttttttcaaa agcaatcaaa
    20701 gatatcgaat tgatcgacga acgctatacg attttacaaa tcggtaactt caaagaagag
    20761 aatgctaaga tgtacatcat tcatgacaac gagacagacg aattaactta caagctcctt
    20821 cctgtcgatg aaaaagctga ctacgaacag ttgttaaaag aactctaaac gccactaatc
    20881 tcctaagtac caagcttttt aataagtaga tgctacataa atacttgaaa aaaggcctgt
    20941 ttgaacataa gaatatccga acggtgatct gcgtttcttt catatagaaa ggaattctcc
    21001 atcactgttc ggatattttt ttattttttt cggttacttt cttctaatac atctgttagc
    21061 caatttgcat attctgtttc ccgattgatc gcatgcgcta aaatcaggta gtgtccgtaa
    21121 ttttcatgtt tttctttttc agtaggaaag atcgtttttt ttcgctctaa tagatgaagt
    21181 cgtttttctt catgaaggtc acgctgctgc tgaataattt ctactaatgc cggatcattc
    21241 acgtctttga cgaaatacaa tttcagaaca aattcgtctc gattaaccgg tagttcattg
    21301 gttggtgtat gaatccactc tgccaattgt tccttccctt cttctgttaa ttcataaact
    21361 ttcttttcca atttttcacc tgtgatctct acttgatgct gaatgattcc ttgctcttcc
    21421 atttttgcaa gttcaggata aatctgacta tgttttgctt gccaaaattc tcctatttca
    21481 tttttgaacg attgattcaa ttcatatcct gtcatcttct tcttacttaa taaccctaac
    21541 aaaatatatc gaagtgtatt ttttcttgcc atcagttttc cctccttgac aaatctttaa
    21601 atccattcta tcataaatga gtaatcgaaa acatgtagtt tattacatgt tttaatttat
    21661 aaggagatgc ttaaacatga aagaatttaa agaattggaa gattttattg gaactcactt
    21721 tatctatacg tatgacaatg gttgggaata tgaatggtat gccaaaaatg accatactgt
    21781 tgattaccgt atccatggcg gaatggtcaa aggccgttgg gtaaaagacc aagaagctga
    21841 tatcgttaaa ttgacggacg gcgtattcaa aatcacttgg acagagccaa cagggacaga
    21901 cgtggcattg gactttatgc caaatgaaaa gaaacttcac ggaatgatct ttttccctaa
    21961 atgggttgaa gaacatccag aaatcactgt tactttccaa aacgaacata tcgcagaaat
    22021 ggaagcagct cgtgaaaaat atgaaactta tccaaaatta cttgttcctg aatttgctac
    22081 gatcacttat atgggtgatg caggtataga caatgaagat gttattagcg aagaaccata
    22141 cgatggaatg ccagatgata tccgtagcgg aaaatacttc gatgaaaatt ataaacgagt
    22201 aaaataaggt tgtgaaagag aggttgtgaa atcagcgttc tgacctgagt attagagaaa
    22261 gaaacggaaa aatagttcct catgttttgc cgttttttga gctaataccg agggtcagct
    22321 gattgaacac cgtttatcaa gaggttgtga aatcagcgtt atgctttgag taataagaac
    22381 gctagttaga aaatagcttt tcatattttc taggctagtg ggcttattac cgagaagcag
    22441 ccgtttgaac accgtttatc aagaggttgt gaaatcagcg ttttgacctg agtattagag
    22501 aaacaaatgg aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca
    22561 gctgattgaa caccgtttat caaagattgt aacaactgca tattcacaca gttaaattag
    22621 tgacttcaaa atgaaataat gctcccagca aaaaattagg ctgggagcat tatttcgtta
    22681 ttctgttcat caaaaatcag cgttttgatt ccatttcatc acaagttcct cctcatttac
    22741 tgcttcatct ttttgctgtg tttgaattgt aaaaccttgt gaaagataga aatgaatagc
    22801 tgattgattt ttttgataga ctgtcaaagt cagtgtttta ttggtttctt ttattttttt
    22861 cattagttgc gtaccgattc tttgttgccg gtattccctt ttcacaaaga ttcctgcgat
    22921 aaactgatca atcaccccag caaaaccaat aatttcttga tctatttctg ctaagtaaat
    22981 cactgcatct ggtaaagctt tcctaacaga ttctacttga ttctcccaat aagttgatgg
    23041 aatgaattca tgagaatcca agttagcatg cagccaaatc gatagaacag attccagttc
    23101 tttggcagac aattttgttg ttgtgcggat cattttttct ctccttactt attcattgat
    23161 tttaaaataa aaaccatttt tttgaagctc ttttacaagt tttcgcccct caaaaaagta
    23221 tatcttctct tgttttactt ttctaatacc aaaagaaaca gcaactttag gatcatcagc
    23281 tgaaacgatt cgataagttc caccaggcaa ttgatccagc ttgactttcc catgatggtc
    23341 gctttgtact gaaaacggca caccatttct agtaagtgtt tttttcttat gtttcaaaat
    23401 gaatgaacta ccatttaccg gtttccctga cgaatcacta ataataaatg aaaaatcaag
    23461 cactttttta cttaatctat actgatagat tagtcgtccg ataaacacta acagtatgac
    23521 tacagctatt atcgttcctg caagaataaa ccattgccgc atggttcttt cctgatttcc
    23581 ttttccttct tcttttttta ccgattcgtt ataaggaatc cgatgtcctg tcactagcaa
    23641 acgatgcgtg ttgatcatat acggcgtaca tgtaagcaag gtaactaagt cttctccttc
    23701 tgaaatcgtt agaacagacg tgtcatctgg cagtaccact tgtatatttt taacttgata
    23761 agccaatttc tcccccaaaa cagtcaaaat aaacgtgtct ccgttttcta aacgatctaa
    23821 ttcggtaaat aaccgtcgat taggtaaacc gctgtgagca gagatcactg aatgcgtcga
    23881 atttccacca agcgggaaag aggttccttg caagacgcct gctccatttt caaggatcct
    23941 accattgagc cgatcataaa gtgaaatatt taattggatc ttaggtatat tgatacttcc
    24001 gatcagccat tcctctaatt ggctggatgt tgtactttca tttctgttct tttcattgaa
    24061 aggatcagta ccaggattga atccttggtc agttaatcgt tgttttttct cctccatttt
    24121 ctttcgctgc acttcaattt cagctgcggt ccgtttttgt acttgttcga tacgtttttg
    24181 atcaataaaa ctatttaaag aatccacata aaatggatat aaagcgatca gtaaccctac
    24241 gacgatacat attaataaaa acaaacgaaa caaaagtcga cgagactgtt tttgcttact
    24301 ctgcttcaca tgtgtcctcc cttgcaagtt tttttgttct ttcttttatt atatgggttc
    24361 aggaaaaaaa tagaaaatga aagccgatga ggttgtgaaa aagctgtcct gcatcaagta
    24421 ataagtacag ccaaaaaaaa tagctactca tattttttgt tgggttgggc ttattaccgc
    24481 agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaaactgcgt tatgctttga
    24541 gtaataagag cgctagtcag aaaacagctt ctcatatttt ctaggctagt gagcttatta
    24601 ccgagaagca gctgattgaa caccgtttac cgagaggtca tgaaaaagct actttgtttc
    24661 gagtattaag aaaaattctg tttcaaaaca aggagatgca acaaaactta tgtcgcatcc
    24721 tctctcctta gaactttcac ttatgttttt cttgtaaaat tcataaatat tttcatgaat
    24781 tttctaattt cttgcctacc tctcgcatag gatatatgat gaatttatct aaagtttgca
    24841 aaggagttcg acaattgaaa aaatatggcc catttttagc aatttttttc attgctttgg
    24901 catttctcat gcctcattta ttcacacata aactaatttt gggtgctgat gccccattcc
    24961 attacaatcg gttctatgat gccgcgcaac aaattaaata tggggatttc cattattttc
    25021 cttcccttta tggattccag caaagtggca ggatcgtgaa tgccgtttac ggcccatttt
    25081 tcgcttattt acaaggattg atcgtgttgc ttgcaggtag ctggtttcat tatcagattc
    25141 tttcaaattt cattctttat cttatttcag gattttctat gctgatttta ctgagatatg
    25201 tcaatgttcg aaattgggta agtgtaccag tagccgtttt attcatgagt acgtattcta
    25261 ttcaatactg gtctatcaac caaggattca ctagctgggg gacttgtttt tatcctttat
    25321 gtatgatccc tattgctgat ttcgtccttc ataaaaagtt ccctgttatc aaagtcgctg
    25381 tctcagtggg acttatgaca cagatccatt tactgtcttc gatcatgtta gctttgatgt
    25441 atatcccttt ttatctttat tactttttta accagactga aaaaggaaaa gcactgcttc
    25501 aactgattaa gtcggtcttg ctttattttg ttctgacagc aaatatttgg gcaggattag
    25561 ctattgttta cagtgggaat aagattcttc ctccctttgt taacagagaa atgagtcaaa
    25621 aggcgataga tctggatggc agttattggc tccattaccc taaggccttt cctatattga
    25681 tattatcagg ttttttgctt tttattttct ttcaacaaaa gaacacctta ttccaaaaaa
    25741 tcgtgttgta cacaagtctt gttttctttt ttctttctac tagcttgatt ccatggacga
    25801 cattgatcga aaaaaatgtg ccttttatta gtttgatcca gtttccattt cgatttttcg
    25861 tgccatttac gctattgctt ctactttacc ttgccctttc actgaaccag tggtctgaac
    25921 aaaagtggtt ccgtctttta agtatatttg gcattcttat tttttcagca cagacgatcc
    25981 agaacttata ccagcatctg gaaaagtggg aaaatgaaac atttgtcagt cgacatactt
    26041 atctattcga tacgccggaa gaagcaagga aaacattctt cagcaaagat atgtctacca
    26101 gcttgttcgt tttccaaaaa accacacctg actatctgcc aatctataag gagacaaaag
    26161 acaataaata cgaccggtat aacgaagaaa tactagaaaa cgaaacaact tttataaaaa
    26221 cacatccagg cggtcttttg acaatcaagt ggaaaaacac tggggacaaa actgttcata
    26281 tccctgttat cgtctacgat cggactgtgc tacagcaaaa tggaaaaaca ttgactgatt
    26341 atgaagtaac agatatcggg accccaatcg tgaagcagaa aaaaggaatc aacgagctca
    26401 cgcttcatta tcaaacgcca atctatttct atttcattct ttctcttact ttgattggct
    26461 ggtttacctt gctatgttta tttatttatc atagatacaa gcttttacgt gcttaggcaa
    26521 aagggataaa tacatccgat caaaaaactc tccaaacggt ataatcgttt ggagagtttt
    26581 attttaatca gtttttttat gttatatgtt atatttttta ttcgttcaca tctaattcga
    26641 tttccacccc gtagtgatca ctgatgattg gactatcgat cccatcaaaa atccgttgat
    26701 attttttaac ctgcattcct tcagggacga aaataaaatc tatacgcaat ttttctgtat
    26761 tttgttccca accatctatt ttcttttcca cagtagctgc ttttcctttt tcattcgcga
    26821 caacatacga atcttgaaga agaaggcaac tttctcttac agtataataa ccttgagagt
    26881 ctactggatt attgaaatca cctaggaaaa aaagtggtac gcgagtttcc aaaaagtagt
    26941 tttccaagtt tttccactca aaataaaagc cacttttttt atctgtccac caagaaaagt
    27001 ggcagctcga gacaaaaatg tcctgatcat ctatctttgt tttcccaatc aagatcttgc
    27061 gtgtctgata atcttctttc ttttgagatt gggagacggt aaggacctga ctttccaatg
    27121 tttttttcgt aagcagtgca tttccttctt catatttatc ataaccgatg tggctcattt
    27181 cccaactcca aaaatactct tttccgtgtt cctttaaaag ctgaactaaa cggtaggcaa
    27241 aattatcttt acggataggt acttgattct ttacctgaca gaacttcatt agcttcgcag
    27301 gttctacttc ttcagattcc agtaattggt tgatttcttg taaagcaatc acttcataat
    27361 cttctaacag gatcttatct gctaattctt gcaatttttt ctctggttct ggctcaagcc
    27421 agctatgcgt gttgagtgtc atgaatttca ttggttttct ccttgtcttc atttcgaaac
    27481 aaaagggttg tgacaaaatt tttgtcacaa cctctccagc aaatagtgtt ctcctgctaa
    27541 ctcctcagta ataagctcaa tggaccagga gctatttttt gtttggctgt ttttatcact
    27601 tggtgcaggg cagctttttc acgacctctc cactaaacag tattcaaact gctgcttctc
    27661 ggtaataagc ccaaccaaac gaaaaatagg aggagctatt tttgtttggc tgttcttatg
    27721 acttgatgca ggacagcttt ttcacaacct ctccttacat tttaatggag ccgatcactt
    27781 taccagctgt cactgtacct aattgcttga tcactacttc ttctacttta tcgtttgtaa
    27841 atacgatcat gatcgttgga tctttttctt cgttttggat cacatcaaga tccatctcag
    27901 ctaatttcgt tcctgttcct acttcttggc cttctttgac agcaatctca aaagctggct
    27961 gattcatttg gactgtatcg attcccatat ggatcaaaac ttcgatacca tctttcgttt
    28021 ccagcccaat tgcatgtttg ctagggaaaa tacttgtcac ttttccagcg atcggcgcaa
    28081 caacttctct tgatgttgga ataacagcaa agccgtcacc catcattttt tgtgagaata
    28141 ccggatcttt tacttcagat aaaggaatca cttctccaga agctgcggaa actagttctt
    28201 gttctatacc taagtattgc ttactacctg cttgtccatt tcctgtagaa accatttctg
    28261 gctctggaat ctctgctcct gattgcagca gatcttcgat atctgatttc aagacatctg
    28321 cttttggtcc ataaactgct tggactccat tgtctttgac gatcaatccc atcgcccctg
    28381 ctcgtttcca ggcttcttca gtccctactt tttctcgatc atttacactt acgcgtaacc
    28441 gagtcataca agcatcgaca tctttgatat tttcttttcc tcccagcaaa tgaatgatct
    28501 ggaccacttg ctgatctgct gttcctgttc cgctgttaga tacaacggtt tcttcactgt
    28561 cattgtcgta atttccgtta cgtcccggtg tagcaaagtt gaattttttg atcaagaaat
    28621 ttgctaagaa atacgtaaca acaccaaaag cgatcacaca caacacgaag ttgataagat
    28681 ctccacctag acccgctttg atagctaacg gtgtccgagt cagcaattcg atattcccaa
    28741 atgaatggac ccgcaatggc aagatatctg ccatcgcaaa tgcagcccct tggatcactg
    28801 aataaatgac atataatggc acagcagcaa acataaacat gaactccaat ggttctgtta
    28861 cacctgttag aaagacagcc aacgctgctg aaaaatacat tgatttatat cttgctttct
    28921 tatcaggatc gacattacga tacatcgcaa aagccattcc catcaagata cctgaagaac
    28981 caatcatttg tcctactttg aaacgagccg gcgtccagtt atgcaacaca aactcatatt
    29041 gcgacatatc ccctgcacct ttaagattca ctaaatcagt ggcccaagcc aaccataacg
    29101 gatcttgccc aaatacttgt gtccccgcct gtgcgcctga taaaatctca tacgatccgc
    29161 caagctgtgt atagttgata ggaatcgtca gcatatgatg caatccaaat ggcaatagta
    29221 aacgttctaa tgttccaaat aaaaacggtg ctagaatcgg tgcgctttct tgtgattctg
    29281 caatccataa accaaaatta ttgattccag cctgtacgtt tggccacacc acagcaagaa
    29341 tcaatgccac gattgttgac cataagataa caacaaacgg aacaaagcgc tttccattga
    29401 aaaatgacaa agcatccggc aattttctat aattatagta cttattataa gccattgccc
    29461 cgacaaatcc agcgatgatc cctacgaata ctcccatatt caaagctggt gcttccaata
    29521 cacttgtaaa aaatccttta accatgatct tcgtaccgaa cagagtgtgg gtaaatgcat
    29581 cttcattcgc aagcatgtca cttgtgacac caaagatagc cccagtgatc cgattgatta
    29641 aaataaatga gatcccagct gcaaatgctc cgcctgctct ttcttttgcc caacttcctc
    29701 ctattgctaa agcaaataaa atatgaagat taccaataat tgcccaacca atattttcaa
    29761 ttactccgcc cgttgtgaca agcggcgtcc aatcaggatt gatcaaggga attgatttcc
    29821 ctatactgat catcaaccca gctgccggca taactgctac cacaaccatt aaagcctttc
    29881 caaatttttg ccaaaactca aaactaaata tctttttcat tccgtttagc tcctttacgc
    29941 aatcggtttc gttaaacgta ttatagcgtg cgttttcttt tttgtaaatg ctttattttt
    30001 tatttttctg tctataccat tctataaaac ctataatacg tgtgttccct tctataattt
    30061 tctcctaaaa atttattttt ttaagctttt ttctatgaaa tattttcttt tgtctattga
    30121 cttgtgcaat cggttgcgtt tattatgaac gtataaaaca gctcattagg aggagaaaaa
    30181 tgaaacagat caaacgacta tttgctattg atccatggaa aatcagcacg acacaattag
    30241 ataaaaaaaa tcttcgactg caagaatctt taacaagtat tggaaacggt tatatgggga
    30301 tgcgcgggaa ctttgaagaa acttactcag gggaccacca tcaaggtact tatttggcgg
    30361 gtgtctggta tcccgataag acacgtgtcg gctggtggaa aaatggttat ccagaatact
    30421 ttggaaaagt gatcaacgcc ttgaacttta tcgcgataga tatttcgatc aacgatcagc
    30481 tgatcgactt ggcttctttg gaaccagaag acttttactg ggaattagat atgaaaaatg
    30541 gtgtattatc tcgtacattc acaatcacta cttctactaa taaagtacgc atttccttcg
    30601 aacgttttct gagcattgtc aaaaaagaat cagcttatat ctatttaact atagaaatgc
    30661 ttgaaggtag cggaacagtc aaactcgtat caaaattgga tggaaatgtt caaaacgaag
    30721 acagcaacta tgatgaacat ttttggaatg agattgatcg tggaactata gaagaaatcg
    30781 gctatttgac gactaaaact attccaaaca actttgggat tgaacaattt actgttactg
    30841 cagcaatgcg ccatttcgtt aatggaaaat cagtcatccc tgactatcac gaagatactt
    30901 tagcattaca tggttgcttg tctgcagaat tatctcaagg tgagacatgg tcagtggaaa
    30961 aagaaattat cgttcttact agtagagata ttccagaagc gcagcaagca gaaaaagctg
    31021 tctcagaatt aacgaaccag acaaacaatt atatccaagc aaaagaagag caaacagcgg
    31081 cttgggcaga acgatggaag cttgccgatg tggtcatcga aggagacgaa gaagcacaac
    31141 aaggtattcg attcaatttg ttccaactat tctctactta ctacggcgaa gatgaacgat
    31201 tgaatatcgg ccccaaagga ttcacaggag aaaaatacgg cggagccact tactgggata
    31261 cagaagcgta tgctgtccct ctttatcttg ctttagccaa acctgaagta actaagaatc
    31321 tgctgaaata tcggcataat caactgccgc aagcaatcca caacgctcag caacaaggat
    31381 taaaaggcgc gctttatcct atggtcacct tcactggtgt cgaatgccat aacgagtggg
    31441 aaatcacatt cgaggagatt caccgaaatg gcgcgattgc ctatgcaatc tacaattata
    31501 cgaattacac cggcgatacg gaatatttga aaaatgaagg actggaagtt ttagcagaaa
    31561 tcgcacgctt ctgggcagac cgtgtccatt tttcaaaacg agcaaataaa tacatgatcc
    31621 acggtgtgac cggcccaaac gaatatgaga acaatatcaa taacaattgg tatacaaata
    31681 cgatcgcagc ttgggtcttg cgttacacgc gagaaaatta tttaacttat caaaaagaag
    31741 ctgctatcac tatcagcgaa gaagaactag ccaaatggaa agatatcgca gagaacatgt
    31801 acttcccagt tgacgaagaa ttaggaatct ttgtccagca tgacacattc ttagataaag
    31861 atctcatgcc tgtatccgat attcccacaa gcgatcttcc tctgaatcag cattggtctt
    31921 gggacaaaat ccttagatct tgcttcatca agcaggcaga tgtactacag gggatttatt
    31981 tcttcaatga tacttttaca aaagaagaaa agcaaaaaaa ctttgatttc tatgaaccaa
    32041 tgactgtcca tgagtcttcc ctttcgccaa gtatccattc gattttagca gcagaattag
    32101 gaatggaaag caaagctgta gaaatgtacc agcgaacagc tcgcttggat ttggataatt
    32161 acaataacga cacagaagac ggcttgcaca tcacttccat gactggtagc tggttgtcga
    32221 ttgtccaagg atttgctcaa atgaaaactg cccatgatca gttgagattc gcaccatttc
    32281 tgccaaaaac ttggaaagct tattctttcc atatcaatta tcgcggacgt ttattgttca
    32341 tctcagtcac agatgaacaa gttactttcg agttattaga aggttttcct ctagaaattt
    32401 acgtctatga acagtcttat tctttagcgg atcgtctaga aataacgcta agaaaggagg
    32461 aagcaaatgt ttaaaggggt attgtttgat ttagacggtg tcattaccga tactgctgaa
    32521 ttccattatc gtgcttggcg aaagcttggt caggaaatcg gcattacgat cgaccacaca
    32581 ttcaacgaac agctaaaagg cgtcagccga gaagactcac tctctttgct tttagcttat
    32641 ggcggaaaag aacactcttt ttcaaaggaa gaatttgcag aattagcgaa aagaaaaaac
    32701 gattattatt tagagatgat ccagacgatc gaaccaaaag atgtcttccc cggggtcgtg
    32761 cctttactgg attctttaaa agaagaaaaa atcaagattg ctcttgcttc tgcaagtaaa
    32821 aacgggccat tcctacttga aaaaatgggt cttacacctt actttgatgc tattgccgac
    32881 ccggcagaag caacaaacgg aaaacctgcg ccagatatct tcctccttgc agcaaaagct
    32941 gtcggactaa aagcagaaga atgtatcggg atcgaagacg cgcaagccgg tatccaagcc
    33001 atcctctcaa gtggcgctca accaattggc gtgggacgtg ctgaagacct tggagaggag
    33061 atcccgcttg tccctgatac gacttttctt acgatcgatt acttgaaaga aaagtgtcat
    33121 gaccatggat aatccagaga tctccgtcgt accagttcca gagactgaaa taaagaaaat
    33181 cacattgaag aacgactact tagaagtcgt tcttctcaat tacggtgccc gcctgcacca
    33241 aatcttcgca ccagacaaag aaggaaaatc agaaaatgtt ttattgtctt acgatcgttt
    33301 cgaagatgtt ctaacagaca aaagcttttt tggagcaact gtcggacctg tagccggaag
    33361 gatccgggat gcttcttggg gtgagcatag attggagaaa aactgcggat cacaccatat
    33421 ccacggtggc acgaaaggct ggtctttcca attttgggaa gtagaagttt tcaaaactcc
    33481 atcatcaata ggtgtggtct tttacttaaa agatgaattt tctacctatc caggaccaat
    33541 cactgctacg atcacttacc gtttaacaaa aaacgaatta gagatgacaa cctctgctag
    33601 tagccttgtt gaaaccatct gcaatccgac caaccatgcc tacttcaacc tttcaggaaa
    33661 tggaacaaga gacatctatg aacatcagtt aacggtcttt ttagatggtg tgcttgaatt
    33721 agatcaagaa aaactgccta ctggaaattg gacaaaaaaa gaaaaactgc caatcgactt
    33781 tagaaaatcc ccaacactgc aagagatcct tgcatgttat ccagatggac tagatgatgt
    33841 atttcttttg catcatccta ggctttccaa aacttcctta caattattcg agaagcattc
    33901 tggtcgccaa atgactatag caacttctaa taaaagtatg gtattatttt ctacgactgg
    33961 attcgaagct gatttctctg tcaatggaaa acagatgcat tcaaactacg gattagcaat
    34021 cgaaccgcaa gaaatccctg atatcgttca tttccccaaa tttggatcga tcaacctcca
    34081 tcctggtcaa gaacggatca gtcagacgat ttatcggttc tctgcacaat aacaagagaa
    34141 aggtgttttt atcatggcta ttaccgtaaa agatgtggcg aaaaaagccg gtgttgccac
    34201 ctcgaccgtc tctcgtgtca tcaacgatca tcctagtatt tccgagacaa caaagaagaa
    34261 agtccgaaaa attatggatg aactaggtta tgttcctaac atgactgccc gcaatctagg
    34321 aaaacgcatt tccagtgcca tcggcgtaat cttaccccct cttgattcaa aagagcggat
    34381 cggtaaccca ttttatcttg agatcatgga agcaatcaac gaagaagcgc ggaattatga
    34441 tatgactaca gcaatcgcta ctgctaaaaa ttttgaattg ctgttagaga acgtccagcg
    34501 tatgcattta caaaaacaag tagatggttt tattctagtg tattcagata gtcaagatcc
    34561 tgtcattgat tatttatacc aaaaccacat ccctttcact ttgatcggac agccttatca
    34621 tcatgagaac gaaatcgttt atgtagataa tgataaccag cttctaggta aacaagcaac
    34681 agaatttttg atccagaatg gacataagaa tatcctgttt gtgacaaaca cgactcatga
    34741 aagtttgtat ttcgaacgat atttcggtta tcaaaaagca atgatgatgg ctggtcttcc
    34801 tgcgttatcc agtgtagtac tagagcatcc ggaggattat ctgtcctttg agaaaacgtt
    34861 gaaagaaaac gaagccacag cattggtggt cattgacgat atcttcgccc ttcgcacgat
    34921 gcagttggcg caaatgtacg gctatgtcat tccagagaca ctctccgtca ttagcttcaa
    34981 caactcgatc ttctcaacat taacacaccc ttaccttaca agtatcgata tcgatatctc
    35041 cgaattagga cgtgtcgcta tgcaaaagct gcgcgaattg atccatgaag aattgagcag
    35101 tggcgtacgt ttggtcatcc ctcatcgctt gatcaaaaga gagacagtcg ttcctttaga
    35161 aaacccatac taaatagctg actcactaaa tacgctgacc atttatccta tggtcagctt
    35221 tttatgtaaa agaagatact acttctaaca cattgtttat ctgtaaaaaa gagcgtggga
    35281 caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcagaag
    35341 caactccttc ggaaataagc tgaaattcac aaaaatttaa aaagcaattt tcttgagttt
    35401 cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttcctt ccttcaattt
    35461 tttacacgtc gacgattcaa taaaaaaccg cctaatattg taaagacagg tgcaagaatc
    35521 gagaaaaaga cgaatggcaa gtaatgcaac gcgctaactt tcatcgttcc cataatgaat
    35581 gtgccgctga ctccccacgg tacgagagaa ttgacggtcg cacctgaatc cgctagtgtt
    35641 cgtgtcaaat aggtttttga taagccaacg tcatcaaaag aacgcttgaa tgtttctcct
    35701 ggaagaatga tggacagata ctgttctcca acgatcagat tgattccgat acagctcaag
    35761 gcagtaaacc cgactaaacg agaaggtcgg tccatctttt cttttaattc ttgaacgatg
    35821 gtatcaacga tcagatattt gatcagtaac ccacctaatg ccaatgcaag taaaatcaaa
    35881 ctagcagaac ctaacatact catgatcccg cctcgagaaa gcagttcatc tagctttgga
    35941 tcaggtgtat ttgcgacata accattcatc aagatgtctg ctgcttttcc gaccgtcaaa
    36001 cttttctcat ggataaaaga aagaacgaga gcagctgtcg atcccgctag taatgtaggg
    36061 atagccggaa tttttttgat tgccccccca aataaaataa ctagtggaat caatgtccaa
    36121 ggagagatcc aaaagctcgc tttcatgttt tggatcattt cattgatact agctagatcg
    36181 gctgaatgac tgttatgtcc taatatcgta taaaaaatca acgaaaggac gaatgctggg
    36241 aaatccgtat aacgcatacc agcgatatgt tcgaatagat taactcctcc aatacccgct
    36301 gccaagttgg tcgtatctga taaaggcgag cagttattcc ctaaaaaggc accggaaacg
    36361 actgcgcctg tagtcattgc atcattgaat ccaagtaaat gaccgatccc caaaaaagca
    36421 attccaatcg tcgaaatcgt tgtaaatgaa ctgccgacag tcactccgac aattgcacaa
    36481 acaagaaatg ctgtcggtaa aaagaaacgc acagaaacaa gtttaaatcc ataaaccata
    36541 atcgtaggga tggttccaga taaaatccat gaagcaacta agacaccaat caaaagaaaa
    36601 atcaggattg gaatgatccc tggagtgatt cctttgatga tcccgtcatg gatctcatcc
    36661 catgtgaact ttttaaatct gccataaaac aacaaacaca tgaaagctgc aaggatcggg
    36721 atatgtggcg agagctggaa tccgatgatc agtactccta ggagtgccaa caacacgatc
    36781 aatacagcga tactttcttt taaagaaata tttttattca tcgtcgatct cctatttcaa
    36841 atgaaaaggc cctgccatga aaaaactatt cacttggcag gacctcctac taaatcagcg
    36901 acaaaacgtc ggctgaaaga cttatttttt ttatttttct tctaaacttt tttctacctc
    36961 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    37021 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    37081 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    37141 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    37201 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    37261 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    37321 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    37381 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    37441 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    37501 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    37561 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    37621 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    37681 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    37741 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    37801 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    37861 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    37921 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    37981 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    38041 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    38101 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    38161 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    38221 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    38281 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    38341 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    38401 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtctat
    38461 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    38521 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    38581 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    38641 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    38701 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    38761 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    38821 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    38881 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    38941 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    39001 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    39061 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    39121 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    39181 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    39241 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    39301 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    39361 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    39421 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    39481 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    39541 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    39601 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    39661 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    39721 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    39781 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    39841 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    39901 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    39961 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    40021 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    40081 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    40141 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    40201 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    40261 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    40321 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    40381 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    40441 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    40501 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    40561 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    40621 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    40681 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    40741 caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    40801 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    40861 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    40921 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    40981 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    41041 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    41101 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    41161 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    41221 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    41281 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    41341 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctact tctggtcctt
    41401 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    41461 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcagaattt
    41521 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    41581 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    41641 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    41701 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    41761 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    41821 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    41881 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    41941 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    42001 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    42061 tgctagcatg tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    42121 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    42181 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    42241 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    42301 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    42361 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    42421 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    42481 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    42541 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    42601 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    42661 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    42721 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    42781 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    42841 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    42901 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    42961 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    43021 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    43081 tttaaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    43141 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    43201 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    43261 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    43321 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    43381 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    43441 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    43501 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    43561 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    43621 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    43681 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    43741 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    43801 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    43861 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    43921 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    43981 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    44041 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    44101 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    44161 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    44221 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    44281 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    44341 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    44401 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    44461 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    44521 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    44581 ccgtaatttt aaacttttat aatatatata gttcaaattt attctgaggt ttattataac
    44641 tatctattga gaaagtatca agctagcaat tccttgttta tgaattcatt gacagaaatt
    44701 tataaaaaga atattatgta ctaaaataat aaaaaaagtt ttataaaaag gtataaaacc
    44761 tttttattaa actatttaat cttgtttagt atatattttt agaaaaagga gtggggtttt
    44821 tatggcagaa atcgtcaaag tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt
    44881 aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg
    44941 ttctggaaaa tccacgacga ttcggacact tttagggatt ataaaaaaag acggcggaac
    45001 ggcaacgatt tttgggcaag atgtatggga agatagttta tctatccatg aacgtctttc
    45061 ttatgttcca ggcgatatct ctttgtgggg aaatttgact ggaggagcga tcatcgatct
    45121 cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt
    45181 ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg
    45241 cttgattgct gctttggctg ttgattcgga tctctattta tttgatgagc ctacttctgg
    45301 tttagatcct ttgatggaaa cgattttcca agaagaagtt gaaaaattga aagaacaggg
    45361 caaatccatc ctactttctt ctcatattct cagtgaagtc gagcgactag cggataaagt
    45421 cgtgatcata cgcgaaggaa cagtcgtaga agaaggaaca ctcgatgaat tgcgtcattt
    45481 gactcgttcg accatcacta tagaaacaga agcagagaat agtcacttag cacaattgaa
    45541 cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc
    45601 tttcgatgtg attttagctg aagcgtccaa attaggtgtg aaaaaatttg aatctattcc
    45661 accgactctt gaggatttat tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac
    45721 tgataaattt tcacgctggt ccaccctttt ttctcaaaac cttagccgtg actggaaaaa
    45781 gatcctgata tggctagtcg gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga
    45841 agaattgact tccggacaag gattagctgg attatacgaa accttgcaaa acccggcaat
    45901 gattgcaatg gtcggaccaa ctccagcttc gaatgctgct gagtatactt taggagcgat
    45961 gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct gctgtgatct ctgttttaca
    46021 cgtggtgagc caaacgagaa aaaaagaaga acttggtctg attgaactta ttcgttcctt
    46081 tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct gaaagtattc tgatcaattt
    46141 gcttttagca atatcgatca gctgtacgat gatttttttc caagcagatt cgatcactgc
    46201 agaaggtgct tggctttttg ggctatctgt aggtagtatg gggattttgg ggagtgccgt
    46261 tgcactgttt gctgcacaac tgatgccaag ttcagccagt tcgaatggtc tgtctttagc
    46321 gtttttaggt ggactttacc ttctacgagc aataacagat gtagctgatt cctccctttc
    46381 gatgtggaat cctttaggct ggtcttattt gacctatcca tttacaacta acaactggat
    46441 tcctttgctt tatttgtttt tgttcagtct aatcttgata atcagttcat ttgttttaga
    46501 ggaacatcga gatttgaaca ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa
    46561 acgttctctt ttatccttgc gtggtttatt attctatcta aataaaggga tgacaatcag
    46621 ttggcttatc acttttggtt tgatgggggc ggcatacggc tctatctacg ggaatatgca
    46681 acagttttta gaaagtaacg acttaatgaa acagatgttt atccaatctg gcgtctcaat
    46741 tgaaaaaaac tttacaagca cgatcatggt cgtcttgatt tgtctagctg ctattttacc
    46801 aattgctagc atcaatcgtt tatttacaga agaaacccac ggacgtatga atcagctgtt
    46861 cgcaacaaaa atttctagga accaacttta ttggaccaat atgttactag ctattctatt
    46921 tgctatattg gcaatcctga ttagttctgg tgcactaggg ctttctgctt tggcttcgat
    46981 gaaggatgcg aaagacatgg atctattgat gtttcttaaa gccgggttca atttcctgcc
    47041 tgccgtttta gtgtttatcg gaattagtgg ctttctattc ggatggcttc ctcgaatggg
    47101 taaacttgct tatatctact tagcttactc ctttgcactc aattactttg gcggaatttt
    47161 ggatttacca gaatggtttt ccaaaacagc tgtccctagc tgggttccac ggatgccaaa
    47221 cgattctttt gatctttccg tgttccttac gttatcatgt atcagccttg cactgttcgt
    47281 ccttggttct attggttata ggcgaagaga cttactagaa ggataataaa aaagcatgta
    47341 caatttcatg ctagcctcca aatctgggtc aaaaatccaa catttggagg tcattttttt
    47401 atttacaagt cttctaactg ctctttatca atgatccaga cttctcgatt ctttcttttt
    47461 acataaccat tctcctctaa taatttaaat tttctagata aagtttcggg ggtcgtaccg
    47521 ataaatgcag caagttcttt cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct
    47581 ttagctaagt caagcagata gacagcgact cgttcttcta ctttttccat catcaaaaaa
    47641 gttgtctgct gctgtacttt tgttaatttc tcaacgttga ttgctaaaag tttcagacta
    47701 agggcgggat aatgcagtaa gagttccgag aaatcttttt gtctcaatat gcaaatttcc
    47761 gttttttcta gtgcttcgcc aaataaagac tcatttggaa cgcctagcaa ctggctctct
    47821 ccctcatatc cgcccggctc tattactcgt aaaagctgtt cctttccgct agcagataat
    47881 tgatacactt tcatactgcc ggaggccacg atcactaatt gaggttctcc attcggcgca
    47941 agaatctgtt ctcctttttc cacatagtga tgtttgacaa gtgcgtgtac tttttcttga
    48001 tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa cacataaatg atcatgactc
    48061 ataatctctc ctccttctaa acagctattt ctaataaaga aacataactt cccttattcc
    48121 aactgataat tttcttttat catagcataa acgtcaagcg aaccagatac cagcaaatag
    48181 agtggatttc tttttgtccc ttgctttatt ttccttcttt tgagacctta agtaattgat
    48241 tcctatcaat tttttaaata tttacttttt gaataatcaa aaacaaacct tcactcgcaa
    48301 gccgagagta aggtttgttt ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc
    48361 gcacctagct tttgatatag tggtgctagt tcttgtaagg tttcagcggt tatttgctca
    48421 acgtgttcga tttcttgcgg atagaagaat agtttaccca tttgaaaggt tcggtctctc
    48481 atcgcttttt tcaactcttc ttgtttcttt gtatcggaca aatagtcaga caaagctaag
    48541 tggtcgtcaa gttcatgttc tggttgcggc catatgtaat aatccttgat atcgactgtt
    48601 ttgctccact ctttcagtat cgcaagcaac tggttatacg ctgcaatgct accatatcgt
    48661 tcttcgcttt tataatgttg gtacatcaaa tagatttggt attgtttttg attcaacaaa
    48721 actgctagac aggcattctc gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga
    48781 ctgcgtaaat tccagccatt cgtccagctt tcgattttag gtttgctcat gccgtacata
    48841 tcaggcaaaa gtgcagctgt ctggagctga atctctttcc atttttgcca gtgttcttta
    48901 tattctttct tgatttgttc aatcttatct gttggatggc tctctttgag ctgtttgaat
    48961 gcgtagattg gtcgatcaaa caactgataa tctgattttc caagttccat tgtttcgcct
    49021 ctttccgtta tcttttgtat tctacctgaa ttttgcgata tggacaaata gaggttgtga
    49081 aaaagctgtt ctgcatcaag tcataagaac agccaaacaa aaaactgcga tgagcatcac
    49141 cgcagtttta ttgaacttaa ggttgattga tcacatctaa aagcgttgaa aataaaatgg
    49201 cactagaagc agctcctggg tcgatatgtc cgattgcacg ctctcctaga taggaagctc
    49261 gtcctttttt agctaccaag actttggtgt gttcttttgc ctggtcaatg atttcttttg
    49321 ttaaattccc tgccttcaat gcctctgtga ctggcagcca aacatctaac atcgttttat
    49381 cgtcagcttc tgcctttcct cggctttgaa tacccgctgt tccctgctcg atcacttcgc
    49441 ctaatttttc ttgagaatca atagaatcga cctcttttgt cgctttcatc atgttcatga
    49501 atgcagatcc gtataacgga ccagaagccc caccgacttt cgatatcatc gccatagaga
    49561 atactttgaa tgtatcgctg attgtttcag gatgatcggc ttgaaaagct tcttcataag
    49621 ctgaaacccc tcttcccatg ttatttccat ggtctccatc gccaatcggg gtatctagat
    49681 cactgagata ttgtttgttc tgattgattg cctctgcaaa ttgcgtgagc cattcttgga
    49741 tttcttgaac ggttagtttc atattatcac ccttttatct tgagattttt taccatgaaa
    49801 tcgtctgtac attgctttct aaagaatctt tccattgtgt ctccgtcaag tcgatcagtg
    49861 ttagagaaag accttgcata tctagtgagg tcataaaatt accgactttt cggaaaacta
    49921 cattgacgcc tctttcttcc aacagagcta aaacatcatt catgaacacg aactgttcca
    49981 tcaaaggcgt cccgcccatt ccgttgacta gtacacctgc ttcttttggt aaattcgagt
    50041 aatcatctaa aatcttagta acaagttcct ttgccagtac tttagaaggc tgcatctttt
    50101 ctcgacgata gcctggctca ccgtggatac caacaccata ctcgatctca tcttctccta
    50161 gttcgaatcc tggtttccct acttctggta cagttgctgc tcgaagcgct agaccgatcg
    50221 tcttagttgc ctgtacgacc ttttctccca gttctttcaa ctcagctagg gaagcaccgt
    50281 ttcttgctgc atcgcccacg attttgtgta caaggacagt acctgcaact ccgcgttttc
    50341 cagcagtata cgtactattt tcaacggcga tatcatcatc tacgatcaca ctttctactt
    50401 caatatcgtc catagcagct aattcctttg ccatatcgaa gttcaatgca tcccctgtat
    50461 aattcttaac gatcagcaaa actccttttc cactatccgc ttccttaata gccgtctgaa
    50521 tctgatctgg agttggagaa gtaaacacat caccaagcac cgctgcactt aacatccctt
    50581 cccctacaaa cccggcatgg gatggttcat gtccgcttcc tccaccagaa accaaaccaa
    50641 cttgttccgc tttctgattc ttggctacaa cacgggaaga ttctacacga tgaacaagtt
    50701 ctggatagct ttttactaat ccttccagca tttcttcgac aatattgctt ggatcattca
    50761 tgattttttt caatttattc ccatcctttc tttccggcat tttgtgcttc tgttaagatt
    50821 tgctgagtat catctgttat accggcagtg atggctgctg caaaagctcc ttccactaaa
    50881 ggcgcatcca ccagatgata acgttgctgc tgctcttctt ccaacatgtc ttttgccagt
    50941 tccgagttca ataccgcgct accgatatcc gcaaaaacaa aataactagc tgcatcgctt
    51001 gattcattga ctgcttctac gattttcatc gggtctgatc cgatctcgcc atttattccg
    51061 ccgagagaaa aaatttgcac ctcttcggat ttttgcatct gctcaatcat ttctttaata
    51121 ccatctgtta ctttttgact atgagaaata agcaaaatac tttttttcac aagaatcttc
    51181 ctttctttta catagttgct aacgattggt ttcgctttca ttaatgaaca attcaagtat
    51241 ataataaatt tctaattttg ggaagatatg tgtagtgctt cttgaagttt gttatacttt
    51301 aattaaaaga gaggagtgat cgaatgatta caacaacaac gaacagtgta gagggaaaag
    51361 ccgtagtagc ttataaaggg atcgtttttg gtgaggtcat tactgggatc catgcattta
    51421 aagacttgga ggcggggttg cgaaatatct ttggcggccg ctcaaaaagc tatgaaaacg
    51481 aactaatggg tgcaagagaa gaagcacttg ccgaaatggc cgaacgtgca aaagcattag
    51541 gagctgatgc gatcattggc gtaaaaatgg actatgaagt attaggcagc gacaatggca
    51601 tgctgatggt cacatgtagt ggaacagctg tctccacgag ataagtaggg gacatctgtt
    51661 ttaataacta aagagagatg ggacatgtag tggcactcaa gaattagatt tttcagtcta
    51721 acttcgaggt actctacctt cccatcctct cttttttatt tctttttgcg gatgattccg
    51781 taagctgctg cgactaacac acccgcccct gctaaggaat aactgacaga agctgttttt
    51841 tcatttgttt ttggcaaagt ctgacttgtt gatttggatg gctctgtcgt ttgctgaact
    51901 gtttctgtcg tgcttccaga ggtcactgat ttttctcctg ccgaatgcaa aatatctttt
    51961 attcgttgga aactagctgc taagtcagct ttttttgctt caaaatcatt agcaactgtt
    52021 tgtgcttcgc tgatctttcc gtggattgct tgactggccg ctgtgatatc cgcttgagtt
    52081 tgacttagct ttccagaagc tgtttgagca acctgattta attgctgatt gaattctttt
    52141 gcttgttgga tcttcccgtc aagatctgtt tggacagctt ccgcttttgc tttcgcttcg
    52201 gcaagctttt tctcaagttg tgcacggtca tttcccactt tatttttaag atcagctaaa
    52261 tcttgatcga ttgctgcttg gtcttctttc aatttgtttt ttgcagcttc taatgcttct
    52321 tcgagttctt ttcttttatt ttccaagtct tgcaaatgat cgatttggtc agaagaggta
    52381 gaactattta ttgatgtatc ttctgaagat gaaatcgttt ctcctgaatt atctgtcggt
    52441 acattggaag tcatttctgt ttccgaggca ccgttttcag caccagaaga gcttatagtt
    52501 gttgaaggtt ctgtttgagc aggttcagaa gtttccgcaa aagctgtctg tcccaataag
    52561 aaaatactgg aaacagcaat tggccagatt aacggcttca ttcttttttt catcactttt
    52621 cccatcctta ctacttttta actaatttct tattataagc aaaaatgttt ggaagaacca
    52681 taaacacaac aggctgtttg tctaaaaaac ggtgcatctt ttattttttt gtacgttccg
    52741 ggcttttcca tgtcccgctt ccataaccta aaatcgtttt gactgctgga atgaaagtcg
    52801 tcacgatcgt tatcgcgttc actatccaga acaaaagcat atatagtggt gcgaaaagaa
    52861 ggtatttcaa ttttcttcgt cgatcatcta caatcagcga agctagcaac tggaaaaatc
    52921 ctgcgaccat ttcaaagcag atgaacacaa aactgaatgt aaaagtaata taaatctgtt
    52981 cgtagtttcc ttgatataca taatgcccga ttaatccagc aaaaagaact acggaaatac
    53041 aaaagaatat tgaccagaca atactcaatg tttgatcaat gaagatgatc gttcttccaa
    53101 tatgtttaaa aggatggagc atcacttttt tgaaattcgt cagccagact tctgttccac
    53161 cttttgccca acgttttctt tgtcgatata gcatcttcaa cgtcacaggt acttccatga
    53221 aaaagataat ctcaggagca aaaacagata accagtcatt taactgatga tcccaagcaa
    53281 tactgatgtc ttctgtcgct cgatcttgtc tgaatccgcc cacatcgatc aaggcttctt
    53341 ttcgatacat cgtatttgct ccactgtacg cataaagacc accgaaaaca gctgactgtg
    53401 ttcgtttaat gatgcccacg atacttgaga attcaaccgt ttgagatttt gcaatcaatt
    53461 tggtacgatt ttggacatcc atatttgcag tgaccgcagc aatatgtctt gcaccagggc
    53521 gaatgaaata attcacatac ctaatcagtg catctggctc cggtaccgtg tctgcatcat
    53581 tgcttaaaat caatttccct tttgcaaagg caagtccgat attaaacgcg tgggcttttc
    53641 ctttattttt ttcgatccga accacacgaa gattagcgta ttttttcatc agtctagcta
    53701 atatttctgg tgtttggtcc gttgaaccat cgtctgtcac taatacctca tagttgtgat
    53761 aattcaattt cgtcatcaaa tactcgattg tatcttcgat cacaatttct tcattatggg
    53821 ctggcaccat gatcgtgatg aacggttcga cactcaaggg aacatctacc cattcttttt
    53881 gcttatgttt gtataaaaac acataacacc agacaccaat aaaccataca aatccgccta
    53941 aaatcggata agaaacgaga aaaaatacat aatagacaaa caagtatcta agaataaatt
    54001 acctgtatgg gtgattgaga tcatttcagt tccaccccat tttctttata caaatctgca
    54061 atgaatgtcg tatctagatt tttctcttcg gagacagaat aaaaacggac cgtttctctc
    54121 tcttcttttg ttccaaaacg ttccgtatac acttcttcaa gtaattcttg tctttttttc
    54181 aatttttcat cttcatattc aaaagattta tttacttttt tgtatagatt tcgattgttc
    54241 cacctagtca atacaaatga ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg
    54301 attaagaaat acagcatttt cttttctgat aaaaaagcgt acaaggaaaa atccttggct
    54361 ctttctggtc tcgttaatgg gaaaaagacc catataaaag gaagaacaac gatcatccat
    54421 cctaaaagag ccactaacgt ttgccaaatc tttaaccagt aattaccttt tgaaaaatac
    54481 ttatctttca ccaaaattga tgcaacttct tctcttgtta cttttttcat atcctcacct
    54541 cacgtttttt tgttacgacg taggagttta ccatacattt catttctttt cacataaagt
    54601 gttcctaaat ttttcctaag aaaaagaaca aaacatttct catcagcaaa tgaaaaatat
    54661 tttgttcttt ttatcagttg ttctttttat tttttatctc ttaattatgt tgctctttcc
    54721 aatacgacaa tgcttttcat tcctctagat atcgccgtgt acatttgtct gcgtcttctt
    54781 tgctcatctc catcgttttc aaatggatat aagattacat gatcaaattc cagtcctttt
    54841 gctagactca aaggaatgat ttggcagttt tctaatccat atgaattttt tttaatcgat
    54901 tcttgtagcg tttgggtttc ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt
    54961 agagaaacca aaatctcagc tagtattttc aacaattctt cagaggatcg aaaagagcgg
    55021 tattcgggtt cttttccttg cggtcggatc gatacgatgt cgatcttctc ttgatccaca
    55081 gcataggttt tgaaaagctc tgtgattgct ccgctagaac ggtaactatt tctaagatca
    55141 taccgatgga tcggcagatg gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg
    55201 gaagaattaa agattgcctg attctcgtca cctactaaag taaagtgagt ttttggaaat
    55261 aattcgatca atagacctaa ttgtgccagg gtataatctt gtacttcatc aatcaataga
    55321 tagcgaaatg ctggtacatt taccttttca acaagtaaat gacgaatagc taatataatg
    55381 actgcttcat caacagtacg tgtgggttgc caagcataag ctgagccata tcgtttttca
    55441 taaattcttt caaacaactg atgctcatcc acccatgcca tttcttcgat ttgtctggtt
    55501 atttttcgat attttttttg taaaagtttc cttgcataag ctacaatact ttgttccgaa
    55561 tcatcagaaa tcaatttacc gaataattcc aactgcctat cttctgtcaa cgagagtacc
    55621 tgattctgca cagcaggact ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc
    55681 agtgcctttt tcgttgcttg aagcctatcg atcaatttag gatgagcagg aactttctca
    55741 tatatttttt cgatcgtttt tttagaaagg actgtttttc catttttagt aagtccacgg
    55801 ataaaattcg gatgatctaa aaacaaagga tctgattgtt ttagattatc gataaacttt
    55861 ttgctacgca aacgttctgt ctgctccgat acattttctc ccgtgatacg tttgaaataa
    55921 gcctcttcac cttctatttc ctctgctgaa agctgtgaaa ccagctgtat gatggttaga
    55981 ttcagtggat ttctttcacc taatgatggt aggacattcg aaatatagtc gataaaccga
    56041 tgattaggtg atagaatcag caagtcatcg gatgtcatcg tatctcgata taaatagagc
    56101 agatacgcaa tacgctgcat gatagcagag gttttaccac ttcctgctac gccatttacc
    56161 aagatatttt gacttttcac atcccgaatg attgtgtttt gttccgattg gatcgtagca
    56221 gtgatagcct tcatttcatt ggtctcatct tggcctaaga cttctaataa tacgtcatct
    56281 tgaatagcta cagacgtatc aaaaaacttg accaaacgat ctctttccaa aagaaattgt
    56341 cgtctttctt taataaccgt atcgatttta ttttgacgaa ccgtataaga agatggacca
    56401 atcatgttat tgtaaaaaag ttcggcaatt ggtgaacgcc aatcataaac aagattttca
    56461 tctgcatcat ctgtgaaatt tgctgtaccg atataaaagg attcttttgg ctcattatcc
    56521 agaaagtcta caacgacttt tccaaagtag ggacttttca ataaacgttc gatctttgct
    56581 agctgatttt ctgcgttttt ctgtttgata ttcatctgat cgatttctct atttttcatt
    56641 tcaatcatcg aaaaagtatc caggttatct aagtaactgt caaaattcaa tcgaagctcg
    56701 ccagccattt ctttcaacga ctggtttcct tctatttttg ccgtatccat cgattcccgc
    56761 agttcgtttt ttttatcaat tagcttttga tacacgttct tcagatatgc ttcttctaac
    56821 tgtttttgtg tgttgaccat acacattctc ctcttttttt aaagtggtgg aaccgattag
    56881 tttacctctt ttaagagtcg aatgcaagct ttttaacgag tatcatttgt taggatatat
    56941 cagttcttca ggtcggacag tttgatataa agaaaatgca ccatcaaggt tttttacgtg
    57001 gtagccagac tgcttcaaga ttctttccgc tagatagcta cgtaatccgc tatgacagct
    57061 gacgatatat tcttgttggc tgtctaattc atttaatcgg tcccgaagtt cattcaaagg
    57121 gatatgtttg gcttccttga atttgccctt ttccaattct tctgctgttc gcacatctaa
    57181 taaaattttt ccttttgcta gttctgtcgg caattcatgc cattgtacgg tttcactaag
    57241 cccttctgca atgttcattg ctgcatatcc tagcatgttt actggatctt tggctgagcc
    57301 aaatggcggt gcatacgtca attctaattc cggtaaatcg aagatcgtca aatgcccttt
    57361 gattgctgtt gctaaaatat cgatccgttt atccacacct tttgccccaa caccttgtgc
    57421 accataaatc tcgccagtct ctggatggaa gatcaacttc agtaaaatat ctgttgcttc
    57481 tggataatag cttgcatgat cttttcctga tacatgaatg acttcaaaag acagttcgtt
    57541 ttctttggct gttctttcac ttaaaccggt ggatgcggca gtcattccaa acgttcgtat
    57601 aattgctgtt ccaataccgc cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac
    57661 ttgtctacct tgacgattgg ctggtgaagc aagagaaata agtgcatctt ttccagtgat
    57721 ttgctgtttt acaacgatag catcccccac cgcataaata tcaggaatat tcgtttgata
    57781 acgttcgtct actaggatac ccccgcgtat gcctaattcg attccagcgg tcactgctag
    57841 ctggttatca ggacgaacac caatggataa aataactaaa tcagcaggga tggtacgccc
    57901 atcatccagt cgcaatcgtt tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat
    57961 cacttctact ccttgatgag acaattcttt ttctataaaa gctgccattt cttcatccag
    58021 tgttggaaga atatgtggtg cttgttcgat caccatgact tctaaacctc ttctttttag
    58081 gttttctgcc atttccaatc cgatgaatcc tgcaccaacg atcacggcac gctttggctg
    58141 tttttctaat gcatgcatca ctttatcgat atctggtaca tttctaatag aaaaaacatt
    58201 gtcggcttct gttatccctg gaatcgatgg aacaactggt tttgcaccag gagataaaac
    58261 caatgcatcg tactgttcaa tgtgtttttg accctcatgt acgacttcta ccgttttgtt
    58321 ttcgggaaag atggctgtca cttcatgatt tggacgcaca tctaaacaaa aacgagcgga
    58381 taatgattct ggcgtttgga caagaagatt ttcccgttca ctgatttctc ccgaaagata
    58441 ataaggcaag ccgcaatttg caaaagaaac atacggtcct ttttcaaaaa caacgatttc
    58501 tgcatcttcc attaatcggc gaagccgtgt agcagcagac attccacctg ctacacctcc
    58561 gacaataacg attttcatct tatttccctc ctactgttcg atcaaaccat tgattcatgc
    58621 caccacgtac attcactaca tcataaccag atttctttaa ttctttagct gcttgtttac
    58681 tcctcatccc tgattgacaa atcacataga cggtcttttc tttgtctcca tcatatctgt
    58741 ttatactttg taatggaaca tttttagctc cttttatatg cccgccccgg tattctgcag
    58801 gtgttctaac atcaagtaac gctggatttt ccaaaagctt ttcctttaat tctgggactg
    58861 ttatcgattt tattgattga aatagcaaat tcattttctg cacctcattt acatttttat
    58921 aaatacctca tggggtatat taaacaggag ctttattaat atgtcaacca gaatgactct
    58981 tcattttttt cactactttt tatatttgca taaaataccg ataaggggta ttatctaatt
    59041 ataaaaatag aggagaatgt gttatggaga ttgataataa aaatatcctg aaccgtttga
    59101 aacgtacaga gggacaaatt cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta
    59161 tggatgtcat cactcaatta agcgctgttc gttctagtat tgatcgcgta atgggcatga
    59221 tcgttgcaga taatctgaaa aattgctttg agaaacctga tagtaatcca gaagaacaag
    59281 cggctaaact agaacaagcg atcaagatga ttattaaaaa gtaaaggaat gatttcaatg
    59341 aaagtcgttt tccatattga tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa
    59401 aacctgataa aagcttctcc agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt
    59461 accggttatc tagactctgc caatgcagaa ttcttagatt tgcaaggagt cgtttttcac
    59521 gcttgcgcca atgctatgcg tgctaatcat atttcagaaa gttctttacc tgaacaagta
    59581 atcgttgtac cagcaggtgt ccttgattta gttgaactgc aatcccaagg ttatgcttat
    59641 atcaagcctt agtcagtaga ggttgcgaca gaagtgatca gcttcaagaa atttcggctt
    59701 atttttcgaa gaagctactt ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct
    59761 gcatcaagta ataagaacag ccaaacaaaa atagcttctc atatttttcg tttggttggg
    59821 cttattaccg cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg
    59881 gtttgaccta agtattagag aaaaaaacgg gaaaatagct cctcatattt tgccgttttt
    59941 tgagctaata ccgaaggtca gctgattgaa caccgtttaa ggaagagatt gtgaaaaagt
    60001 cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg atttctacat ttttgcataa
    60061 atttggctta ataccgagaa actggctttt gaataccgtt taaggaagag gttgtaaaat
    60121 cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagctgctca tattttgccg
    60181 ttttttgagc taataccgaa ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc
    60241 ctaccattta actccgaatc acaagaaata atttcaaaaa caacttctcc tgtttttggt
    60301 ttggttggct tattatcgag aagtagctac tgggagtcta tttatcaaaa aaaggaatgc
    60361 gacataggtc acaccccttt tttattttct ttgaaacatc tttcggtact cgacagcata
    60421 caagacaaaa gattgtaaaa gaccgtaagt gatgacggca aaaatgactc tcacccacag
    60481 cgttggtaat tctgggaaaa gcagcaaaag gatcatgcca acatagatga cagcagtcgc
    60541 tactttgcca taccacttcg ctccacttaa tttgatattt ttagatagcc cgattaatcc
    60601 agctacaaac ataaagcctt cttttatcaa gaatagacca aacaacagcc aaagccacgg
    60661 tcgataccat gccatagaca aaatcagaaa aagttgtgtc aatttatctg caaacggatc
    60721 aagcaccttc cctaagtctg ttacctgatt atatctccga gcaatgaccc cgtctagata
    60781 atcagacaaa cctgagacaa caatgaccgc tcctgctata taaaatgact gtctcagcac
    60841 aaaataaaga taaatcggca gcaaaaaaat acggaatatc gataaaagat tcggaatcgt
    60901 ttgtatttct ttacgccagt ctgtgcttaa tttcatttta tcgccctacc tcctcagcac
    60961 tcttttcttt ttattttagc ttacacgggg aataaaacca atgatttcac tgtttttatt
    61021 tttctttaca gccactgata ttatgagcta atttgagata atcacttgct gttttcatcg
    61081 tttactcttc tttcttcctg tcgtttgcgc atttctttta ctagtacttt atgagctttt
    61141 tttgatcctc gctctaggtc acgctgccgt ttacgtgttt tataatcgat aaacagactg
    61201 tctaaatcgt aacctgtcgg gtacagatct tctgctgaac gcaccagacg aacacgctga
    61261 cgaggcacgt ggattatttt tttgtctaaa tatacatcaa tcgtatcttc acctatatct
    61321 tcataaacta acgcttcttt ttggtattca ttgacaaata tccgatcacc ttttgcaaag
    61381 agctggcgtt cttttttatt ctccttccta tttgtaactg ctgtcttagg aagaaattct
    61441 tttttctgtg tccgataggc tttttccgcc aagtaacgct ctgcttgatg gatcaatttc
    61501 atcgacatat ccattttatg agcaatccac aaagcttggc tttctcctgt ttctccaacc
    61561 cgcaactgat atttaggacg aagtgcttcc cgatcgaaag ccatagcagc cggcacaaaa
    61621 tcttcatgat ctccggcgaa tttcttgatt tctccataat gggtcgtggc aatgatcaat
    61681 gccccttgct catacataga ttccatgatt gcgatcgcta gtgcagcccc ttcatttggc
    61741 tctgttccac tgccaatctc atctaaaagt accaacgtat tcctttttat ctttttcaaa
    61801 atagcagcga tgttttgcat atgaccagaa aatgtactca acgcattttc taaattttgg
    61861 tggtcaccga catcgacgaa aatctcatca aagacagcaa gctctgtccc ttcttttgcc
    61921 ggaacttgca taccgaacat cgccatcaaa gtcaataaac cgactgtctt caacacgact
    61981 gttttgcctc cggcatttgc tccggtgata atcaagcctc gatagtcttt accgatttca
    62041 acatccaatg gtaccgcatg ctcaggtaaa aatggatgtc tgccttgctt gataatgata
    62101 tgttccgatt tattgattct aggtgtgctt ccattgattt ctcggctaaa ttttgcccga
    62161 gcaaagatga tatctaacac cgtgatcgtt tcgatcaata aatcgatcac ttcttcgttt
    62221 tctgcgatag caccagtcaa tgccgccagg acttgatatt cttcactgat ttcttcagct
    62281 tttaacaact gatagtgttc attcaattta gaaatcacag ttggttcaat aaaaaccgtc
    62341 gttcttttat ttgattcatc gataatcgtt ccatctattt tatttttata gctggctttg
    62401 ataggaatcg tatagtattc gcctttttga acaatcattg cttcttggat catttctttg
    62461 tttttcggat gacgcaagaa tttcagtaat tttgactgga tttctttttc gattagttgt
    62521 aattgtttgc gtactttacg caaattacgt gacgcgtcgt cactgacttt attatttttt
    62581 attttctggt caatcaactc ttctacattt atcaaatcag gaagatgttt gctataagca
    62641 aacaacaagg gtgtctggta ttgattctta tcaaaaaact ttgtgatcat ccgactgctt
    62701 ctaagaaaat ccgcatattc gatcaaatcg gttggctgca gaatcaaccc ttttttgact
    62761 tgttctgtca gtgcatcgat cctcggcaac cccataaatg gaacatgctg actgctttct
    62821 aagatcaagc gtgcttctct tgtttcttct tgacgatcca agacagtttg taggtttgtc
    62881 gagaccgtca tatcgctgat tcgttttttg ctatagtttc caattgctct tgcttggatt
    62941 tcctttttga tccgatcaaa ttgtaattga ctaatcattt cgttgtccat attctctcct
    63001 tttcttgaag agatacctgt cccaaaataa ataagggcct agagataaat aaaaggatag
    63061 aacacagctt aaaaaagtca tgtcctatcc ttattagaat acaaagatat tgttttcctg
    63121 acaggtaccg tttcataagc acaccaaata aactttgttt attggctatt cgacttactg
    63181 attagatacc agctacactc acaaaacata ccccattctt ctaggtctta tttattttcc
    63241 acttaactat agcaagacac attttggttg tcaaacttta gtatgtttta tagatttaca
    63301 ccattaatga atagacacct accgcgataa ttgctccgat cattggacct gcgattggta
    63361 cccatgaata tgcccaatct gaagttcctt tattagctat tggcaatacc gcatgtgcta
    63421 atcgaggacc aaagtcccga gcagggttga ttgcatatcc tgttggccct cctaatgaaa
    63481 gaccgatggc taagatcaat ccacctacag cgaatacgtt cgttccaggt gcaaattcgt
    63541 tttgtccaaa agctagcaac ccaagaacta atacaaatgt accaattaat tctgtgatca
    63601 catttgctgg ataattccgt atagcaggac ctgttgcaaa cgttcctaaa atcgcgcttt
    63661 catctttcgt tgcattccaa tgtggcaaat aagaaagcca aacaagaatg gcccctgcaa
    63721 aagctcctaa aacttgcgca atgatgaaag gaacgacaag attccaacta attgcccctg
    63781 tcatcgccat agctagagag actgcaggat tcaaatgagc tgggcccatg aatccagaaa
    63841 tatagacagc cattgttaca gcgagtcccc aaccaaatgc gatgacaatc cagccagaag
    63901 cctgtgcttt acttttgttt aaattcacag ccgcacacac accatctcca agcaaaacta
    63961 ggatcagtgt cccaataaac tctcccatga tttgagtcat atcactgttc atttatttct
    64021 ccccctttag ttcccgcaga tctgattctg caatcaatgc ttctaaacgt ttagtctgtt
    64081 gcgccttctc ctcttctgtc cattcaaaat atccagcaat tgcatcgatc acaggttgct
    64141 tgatctcatc aagctgatct cgttcaaaca acagatgatt tgtacgacga atgagataat
    64201 cgcctggggc caaaaccatt tcttcttcta aaccataacg taaccgagct gactcagcga
    64261 gactcaaacc aggataaggt gccatttctt ttgctaattc aaagatacgt cgggcattag
    64321 tcccgtaaaa atcagcgata taagtagcat cttcttcctc taaaccggct gctactcctt
    64381 cttttgctaa ttctgtcact gtttcttcta atttagttgg atcgaagttt ccacctgaaa
    64441 tctgatattt tttagagtcg atttctttcg tctctattcc gtattcttct tttaataatt
    64501 ggcgtatcaa tcgcaaagct ccttctgcca tcttacggta atcggtgatt ttcccaccag
    64561 ataaagtcag caaaccatct ggttctcttt ctaaagaact acctctggaa atcgtagaag
    64621 gtgctttttc atcacggctg ttttccaaat ggttcaacac gtcttctact tcagcacgag
    64681 aaactttgtt ttccttatat tcacttactg tatcgacaac tttattgaag ctcttgtctg
    64741 aaatcgatcc attatctcca ccattgtaat cagaaccaga attaccaatc aacagtggac
    64801 gaagtcctgc ccagcttgct tcgatatcat tcaatgtgat atttgcttct ggatagcgat
    64861 gattgatcac atccaataga taatctacat cttcttgtgt gactttcgga tccgtaaaat
    64921 ctccttggta atccgtatct gtcgtaccaa agtaagtctt gttttctctt ggaatagcaa
    64981 aaaccatccg cccatcttgc tttcctgtat cgaagtatgt gggttgcggt acaggcagtt
    65041 ttttcgcatc tacaactaaa tggatccctt tagttggacg cattttagga gagactggac
    65101 gcgtaaaatt caagtttcgt actttatcta cccaaggacc actcgtattg atcactaatt
    65161 ttgctttgat ttcgatcact tcatctgtca gcaggtcacg agctttgacg ccaacgattt
    65221 gatcgccatc atataaaaag ccaaccgctt tcattttact tactagataa gccccatctt
    65281 cggcagcctt tttgatatta tcaatcacta aacgggcatc attgttgcgg aaatctagat
    65341 agacacccgc gccttttagc ccttcttttt tcaaaaacgg ttctctttcc aatacttctt
    65401 ctggtgtcag cgtataattc tcatatttgg ttcctgtcac atttgccagt ttgtcataaa
    65461 ggtccattgc tactttgacc gagaacatat tgaaggttgt tgctccttcg ccctcataga
    65521 ttggcaaaag cattggatct ggttttggga tgtgtggggc aatactttgt acgactgcac
    65581 gttcaccaac tgtgtcagct actacttcca catcaaatgt cttcagataa cgaataccgc
    65641 catgaacaag tttggtcgag cgagaggaag tcccttctgc aaaatcctgc atttcgatca
    65701 atcctgtttt gattcctgat gctgctgact ggactgccac accggcacca gtgattccgc
    65761 caccgatgat caacagatcc aattcttctt gctgcatttt ttgaatgcta tcttgtcttg
    65821 tcttgtttga aaacatatgg atttcctcct tattcaaatg cttgggtagc ttttactgct
    65881 tttttccaac cgttgtaaag tttttctcgg cgctcttcct ccatttgaac ttcaaatagc
    65941 ttgccagctt cataaaattc gcggatctcg tctgtatctt tccaataacc gacagctaat
    66001 cctgctaaaa atgctgcccc tagcgccgtt gtttccaagt tttctgctcg ttggatcggt
    66061 acattcaaga tatctgtttg gaattgcatc agatattcat tgtttgctgc accgccatct
    66121 actttcaata caggaatctt gattccagtg tcatcttgca tcgtatcgat gatatctctc
    66181 acttgataag caatcgactg taaagtagct ttaatgatat cttcttttgt cgtaccacgt
    66241 gtcaagccaa acatagcccc tcgtgctgcc tgatcccaat aaggagcacc tagcccaacg
    66301 aatgcaggaa caacataaac ctcatcttcg cttgtagagc gtttcgctgc gtcttctgaa
    66361 tcgctagctt tttgcaacat ttgaagtcca tctctcagcc attgaatcga agaaccagcc
    66421 acaaaaatac ttccttctaa agcatagtaa acctttccat ttatgccgta accaattgtc
    66481 gtcaataaat tatttttgga taattgcggt ttttcaccgg tattcatgac aataaatgaa
    66541 cctgtaccat aagtattctt gaccatgcct ggttcgaatg ccatttgtcc gaataatgcg
    66601 gcttgctggt ctcctgccat ccctgaaatt ggtgcttcgc ttccataaaa atgataacct
    66661 tgcgtataac cataaacttc tgagttagaa gtgacttttg gtaacatcac tcgaggaata
    66721 tttagtaaat ctaaaatctc ctgatcccaa tctagctgat ggatattgaa caacatcgtc
    66781 cgactggcat tcgagtaatc agtaacatgt gcttgtccat cggtcagttt ccatactaac
    66841 caagaatcga tcgttccaaa catcaattcg cctttttcag ctcgttcttg cgcgccttct
    66901 acatgatcaa gaatccagcg tatcttcgtt gctgagaaat aggcatcgat cactaaccct
    66961 gttttttcgt ggaaaaattc ttgatagcct tcatttttta agttatctgc aattccagct
    67021 gactgtctgg attgccaaac aactgcatga tagattggac gtcctgtatc tttttcccat
    67081 attactgttg tttcacgctg gttcgttatg ccaatcccag caatctgatt tggtttgatc
    67141 cctgattcga tgaacgctcc ggcaataact gactgtacag aattccagat ttcatttgca
    67201 tcatgttcca cccaactttc ttgcgggaaa tattgcgtga actctttttg agaactgccg
    67261 atgtgtcttc ctttcttgtc aaagataatt gctcgtgagc tagttgtacc ttggtcaatc
    67321 gccatgatat acgttgattc tgtcattgtg attccctcca ttatatgcaa acgctttctt
    67381 taactactct tatcata
//
LOCUS       DACDMM010000002        57871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDMM010000002 DACDMM010000000
VERSION     DACDMM010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.002"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            12..590
                     /locus_tag="IWD16_000061"
     CDS             12..590
                     /locus_tag="IWD16_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9087150.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            590..1519
                     /locus_tag="IWD16_000062"
     CDS             590..1519
                     /locus_tag="IWD16_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9087151.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1516..3252
                     /locus_tag="IWD16_000063"
     CDS             1516..3252
                     /locus_tag="IWD16_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9087152.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3315..4151)
                     /gene="pheA"
                     /locus_tag="IWD16_000064"
     CDS             complement(3315..4151)
                     /gene="pheA"
                     /locus_tag="IWD16_000064"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP9087153.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4148..4657)
                     /locus_tag="IWD16_000065"
     CDS             complement(4148..4657)
                     /locus_tag="IWD16_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP9087154.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4650..5945)
                     /gene="aroA"
                     /locus_tag="IWD16_000066"
     CDS             complement(4650..5945)
                     /gene="aroA"
                     /locus_tag="IWD16_000066"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9087155.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(5958..7049)
                     /locus_tag="IWD16_000067"
     CDS             complement(5958..7049)
                     /locus_tag="IWD16_000067"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP9087156.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7066..8232)
                     /gene="aroC"
                     /locus_tag="IWD16_000068"
     CDS             complement(7066..8232)
                     /gene="aroC"
                     /locus_tag="IWD16_000068"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP9087157.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8234..9298)
                     /gene="aroB"
                     /locus_tag="IWD16_000069"
     CDS             complement(8234..9298)
                     /gene="aroB"
                     /locus_tag="IWD16_000069"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP9087158.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9331..10353)
                     /gene="aroF"
                     /locus_tag="IWD16_000070"
     CDS             complement(9331..10353)
                     /gene="aroF"
                     /locus_tag="IWD16_000070"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP9087159.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(11012..11986)
                     /locus_tag="IWD16_000071"
     CDS             complement(11012..11986)
                     /locus_tag="IWD16_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP9087160.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(11967..13850)
                     /locus_tag="IWD16_000072"
     CDS             complement(11967..13850)
                     /locus_tag="IWD16_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP9087161.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(13868..16093)
                     /locus_tag="IWD16_000073"
     CDS             complement(13868..16093)
                     /locus_tag="IWD16_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9087162.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            16208..17242
                     /locus_tag="IWD16_000074"
     CDS             16208..17242
                     /locus_tag="IWD16_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9087163.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(17309..18322)
                     /locus_tag="IWD16_000075"
     CDS             complement(17309..18322)
                     /locus_tag="IWD16_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9087164.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(18400..18588)
                     /locus_tag="IWD16_000076"
     CDS             complement(18400..18588)
                     /locus_tag="IWD16_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP9087165.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(19028..19156)
                     /locus_tag="IWD16_000077"
     CDS             complement(19028..19156)
                     /locus_tag="IWD16_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9087166.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(19159..20532)
                     /gene="rlmD"
                     /locus_tag="IWD16_000078"
     CDS             complement(19159..20532)
                     /gene="rlmD"
                     /locus_tag="IWD16_000078"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9087167.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(20643..21698)
                     /locus_tag="IWD16_000079"
     CDS             complement(20643..21698)
                     /locus_tag="IWD16_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9087168.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(21708..23138)
                     /gene="gatB"
                     /locus_tag="IWD16_000080"
     CDS             complement(21708..23138)
                     /gene="gatB"
                     /locus_tag="IWD16_000080"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP9087169.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(23135..24607)
                     /gene="gatA"
                     /locus_tag="IWD16_000081"
     CDS             complement(23135..24607)
                     /gene="gatA"
                     /locus_tag="IWD16_000081"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP9087170.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(24607..24912)
                     /gene="gatC"
                     /locus_tag="IWD16_000082"
     CDS             complement(24607..24912)
                     /gene="gatC"
                     /locus_tag="IWD16_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP9087171.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(25018..27054)
                     /gene="ligA"
                     /locus_tag="IWD16_000083"
     CDS             complement(25018..27054)
                     /gene="ligA"
                     /locus_tag="IWD16_000083"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP9087172.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(27071..29305)
                     /gene="pcrA"
                     /locus_tag="IWD16_000084"
     CDS             complement(27071..29305)
                     /gene="pcrA"
                     /locus_tag="IWD16_000084"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP9087173.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            29460..31019
                     /locus_tag="IWD16_000085"
     CDS             29460..31019
                     /locus_tag="IWD16_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP9087174.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(31130..33061)
                     /locus_tag="IWD16_000086"
     CDS             complement(31130..33061)
                     /locus_tag="IWD16_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9087175.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(33079..33993)
                     /gene="pfkB"
                     /locus_tag="IWD16_000087"
     CDS             complement(33079..33993)
                     /gene="pfkB"
                     /locus_tag="IWD16_000087"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9087176.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(33990..34742)
                     /locus_tag="IWD16_000088"
     CDS             complement(33990..34742)
                     /locus_tag="IWD16_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9087177.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(34901..35110)
                     /locus_tag="IWD16_000089"
     CDS             complement(34901..35110)
                     /locus_tag="IWD16_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087178.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(35121..36449)
                     /locus_tag="IWD16_000090"
     CDS             complement(35121..36449)
                     /locus_tag="IWD16_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087179.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(36814..37401)
                     /locus_tag="IWD16_000091"
     CDS             complement(36814..37401)
                     /locus_tag="IWD16_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP9087180.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(37484..38734)
                     /gene="clpX"
                     /locus_tag="IWD16_000092"
     CDS             complement(37484..38734)
                     /gene="clpX"
                     /locus_tag="IWD16_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP9087181.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(38981..40270)
                     /locus_tag="IWD16_000093"
     CDS             complement(38981..40270)
                     /locus_tag="IWD16_000093"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP9087182.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(40424..41356)
                     /locus_tag="IWD16_000094"
     CDS             complement(40424..41356)
                     /locus_tag="IWD16_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087183.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(41513..42172)
                     /locus_tag="IWD16_000095"
     CDS             complement(41513..42172)
                     /locus_tag="IWD16_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9087184.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(42175..43200)
                     /gene="sppA"
                     /locus_tag="IWD16_000096"
     CDS             complement(42175..43200)
                     /gene="sppA"
                     /locus_tag="IWD16_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9087185.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(43213..43503)
                     /locus_tag="IWD16_000097"
     CDS             complement(43213..43503)
                     /locus_tag="IWD16_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087186.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            43912..45717
                     /gene="glmS"
                     /locus_tag="IWD16_000098"
     CDS             43912..45717
                     /gene="glmS"
                     /locus_tag="IWD16_000098"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP9087187.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(45789..47144)
                     /locus_tag="IWD16_000099"
     CDS             complement(45789..47144)
                     /locus_tag="IWD16_000099"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP9087188.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(47168..48337)
                     /locus_tag="IWD16_000100"
     CDS             complement(47168..48337)
                     /locus_tag="IWD16_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087189.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(48334..49221)
                     /locus_tag="IWD16_000101"
     CDS             complement(48334..49221)
                     /locus_tag="IWD16_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP9087190.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(49410..53102)
                     /gene="nifJ"
                     /locus_tag="IWD16_000102"
     CDS             complement(49410..53102)
                     /gene="nifJ"
                     /locus_tag="IWD16_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP9087191.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            53301..53822
                     /locus_tag="IWD16_000103"
     CDS             53301..53822
                     /locus_tag="IWD16_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9087192.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(54521..55078)
                     /locus_tag="IWD16_000104"
     CDS             complement(54521..55078)
                     /locus_tag="IWD16_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087193.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(55257..55868)
                     /locus_tag="IWD16_000105"
     CDS             complement(55257..55868)
                     /locus_tag="IWD16_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087194.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(55981..56238)
                     /locus_tag="IWD16_000106"
     CDS             complement(55981..56238)
                     /locus_tag="IWD16_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087195.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(56835..57113)
                     /locus_tag="IWD16_000107"
     CDS             complement(56835..57113)
                     /locus_tag="IWD16_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087196.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(57147..57398)
                     /locus_tag="IWD16_000108"
     CDS             complement(57147..57398)
                     /locus_tag="IWD16_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087197.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        15944 a        12501 c        10297 g        19129 t
ORIGIN      
        1 cgggtaaaaa aatgccaata cgcgtaattc ttatcgatga tgaacagacg attttagatg
       61 gaatgaaaca gttgatcaat tggtctgagc acgggtttca attaataggg actttttcca
      121 gtccgctgaa agcgcttgat ttttctttgg tacatgcaat cgatatcgtg gtcacagatg
      181 taatgatgcc tgaattaaat ggaatcgaat tatcgcggct attaaaacag caacaacctg
      241 aaacacagat ccttatcctc tctagctatg atgaatttga tatggttaaa gattctttta
      301 aagaaggcgt cgctgattat cttttaaagc ctcgattgag tcctgatttt tttctaaatt
      361 ccttaactgc cgtttcacaa aaaatcaaaa aaaagcccaa caaaagccca atcaccaatc
      421 gttatgagca gatctgcgag aatctttcac agcagattgc tggacaaact cgtccattgc
      481 ttgaccctct tctttttgaa cacaccttgt ttttctgtta tataccgaaa aacggccatt
      541 cagttcaaaa caaggctcta aacaaaaaca gtcgatcacc cattcattga tggatagcga
      601 cgattcgttt actatttatc ctgtttctac cttttcggaa gaatccggct atgtaatcaa
      661 tactgattcc aaagatacac ttaggaagtt tgttcaaaag tttcgtcaag aagtttttcc
      721 aggtgatttt tttgttgttt ctgattcttt tgcaatccaa gatatcaatg aaatattcca
      781 agagatcaga aattgcacca aaggacaacg tttctatcat aagggaaaga accttgctta
      841 tcagcatgaa cttcttccgt tattaaccaa ccacgagcag caagcaagta gttatttaac
      901 aaaaatcgtt cataaagact acgctttagc tgtcaaagaa cttgagaatt tcttcacgac
      961 gatattggaa cagcctattg atccagctta tttgaaacat gaggcaatca atcgtttcta
     1021 tgcattactc aacgctttgg ctgacgagca tgttgggaat gaagaacttt ctttgctcaa
     1081 gatcgccctt ccttctttat tagccgatgc tgagtatttc gaagattttt ctattcttgt
     1141 caaagaaaca cttgagaaac tcacccactt ttctgcaaat gcaaaaaaag acaatttcga
     1201 tatcttgact ttgatctacg agttcgtcca ggaaaattat gagaaagaaa tcaatttaca
     1261 acttttatcc gaaaaatatc atttttcata tagctatctc tcagccatct ttactgaaaa
     1321 atttggtatc aacttttcaa aatatctaaa aaaagtccgt atcagtaaag ccaagctctt
     1381 tttgacggaa acacggctta ctttgacgga aatctgtgag aaagtcggct ataccgaact
     1441 gggttatttc tccagagttt ttaaagaaga gacaggaatg acaccttctc aataccgtaa
     1501 aggaactttt gtaaaatgag aaaaaaaatc aaagagcatg aactctttgc caaacttctg
     1561 ttcattttgt tcatcggtct ttttctgcaa gccatcgtca tttctttgtt tatttatcat
     1621 cgatcaaggg atgcctatat ccagctgttc aatcaatcga atgatgtggt actaaaaaag
     1681 attcaatctg aatttgaaag tttgaatgat acaatcgaga atacattagc cgcttttgat
     1741 tctaatccag ctgtcaaaag ctatttttcc aatgatccag cccaacatat ggaacagctt
     1801 cagcaactaa gaacaatcca aaaaatgaat gactctcttt caaaaatcca tccaatgatc
     1861 gattacgatg tattgatctt tggagaaaat ggaagaactt ttgtcggaaa cgacatgctc
     1921 actgccgtat ctgccgattc tttttttcaa tctgcgattg ctcagagagt gaatgaacgt
     1981 gctgctgata cacaaatgct ttttgcacac cacggattga ctcttcgcga taagaaagca
     2041 cccagcgtct tctttgtcaa aaaactgaaa aatacattga atcacgttta cggatatgct
     2101 gttttatcga tctcatccaa gcaactagcg aatctttttc aatctgtagt aaatcctgag
     2161 atatcgaaga tctccttcat gaacgaagaa cagacgatca tcgcatcaaa tgaagaaaat
     2221 acaattggga gccgatcaat gattttcaaa aaactaacag taggtgaaac aacgatttca
     2281 aatggacagc agttgacccg tcttcctctg taccgccagg attgtacatt actcagtcaa
     2341 gtcgatcttc attctttatc taaccaaatg ggcgtgattt ttccgattat tttgtataat
     2401 atcgtgacac ttgcttttgc tggctcattg gtatttatgt atttggatcg acacacaaaa
     2461 tcgatctacc gcttgattga ttcgctaaaa cggctagagg aagatggctt aaaagctact
     2521 gttcctgtac aaggaagcca tgaagtccaa ttgctaggga atacgatcaa tcaattgcta
     2581 agtaatgctc agcatcatta tgaacagacg atccaagacg aaaagaagaa acgttcgctt
     2641 gaaatccaaa ctatgcaagc acagatacag ccccatttta tttataatac gctgactagc
     2701 ttgaaatttt tgatttggca gaaaaaaaca gatgaagcag ttcaaggaat cgacagtttc
     2761 attactttac taagaagtac gattgggaaa aaagaagaag tgattccagt caaggaagaa
     2821 atcaaaagcg tacaaagtta catcgatctt ttgagtatgc gatatggcga aagaatctcg
     2881 gctaaaataa tgattcctga tgaactgcag tcattatcta tgccgaatat gatccttcaa
     2941 ccaattatcg aaaacgccta tcttcatgct tttcacaata aaacgcaagg attcatcacg
     3001 gtgtacggca aaacaaaaaa tcagcttctt ttattcgaag tgattgataa cggcgatggc
     3061 ttcgatactg aaaataccaa acaaaaaaat gatcattttt caggaatcgg catcagccat
     3121 gtagatgagc ggatacgcct aatgtatggc gatgcatatg gcttaacgat ccaatcagtg
     3181 attgggacag gaacgactgt aacgatttgt ctaccgcttc ttcctaaaaa tcaatcagaa
     3241 tatcaaaaat aaagaatagc caagcgctga tttttttatt ttaagttaaa taaatcaatc
     3301 agtattcttg actttcatct acgcgtctct tctactgtca gcactggata acagcccaat
     3361 atttgacttt ctcctccgag catcttgatt tcttggagcg cattctctac taatgtcatt
     3421 ggacggtcca acaacaaatc gatcacgaaa aaatattctc ccaagctagt tttcaatggt
     3481 cgggattcaa tcttacttaa gttgatttcc cgccagccaa aagctgccag cattttatga
     3541 agcatccctg gccgattagc aggtagtgtc aagatgacac tcatttttac tggagctgac
     3601 tgctggctgg tcatctttcg gtcaccgacg atccaaaaac gtgtctggtt caactcatta
     3661 tcttggatat tctcagctaa gatctccaat ccaacatgct gtgctgtttc taatgaagca
     3721 atcgctgctg cgtcttcttt cggatgttct gctacataca ttgctgcagc agttgttgat
     3781 tctgttgcta ctaaaggaac attaggataa tgcgtttcta aaaactgttg tgattgtgct
     3841 agtgcttgtg gatgtgaaag gatttttgta atctttgttg ctggattgcc taaaagctgc
     3901 tgcttgatag gaagaacgat ttcactttta acttcgactt ctggatgttt ggaaagaaga
     3961 tcgattgtgt gatgaacagc tccttccaat gaattctcta caggaacgac agccagatcg
     4021 acttgtttgc gaaacaaagc gcgcaaacaa acagggatgg aagcatacgc agtcaattgt
     4081 tctgtaggaa atagctgaca agccgcctga taagtgaatg aactttctgg ccctagatag
     4141 ctgacgttca tatgcttttg atcctttcta taatggtatc gatgatcttt tctggtgttt
     4201 gattggtcgt gttgattcga tgtgtcgcac tttcttcata ccaagaaagt cgttgtgcca
     4261 ataatgcttt tatttcttct cttgtttttc caagagctaa aggtcgaata gacgtttcat
     4321 cttgttggat acgatcaact agacagtccg cttctgcctc taagtaaaga acaatcggct
     4381 gtgtttttaa aaaacgccga ttttctgcac tttggacaat ccctccacca gtcgcgataa
     4441 ttttgtcttc atttgacagc tttcttaaaa tatctgactc cacttttcga aagctttttt
     4501 caccgtaata gtgaaaatat tcggcaatcg acaattggat atgttcctca atctttgtat
     4561 ctaaatctac atactctttt tgtaaacatt gagctaaccc tttaccgatt gttgtttttc
     4621 cagcgcccat gaaaccaatc agcaaaatgc tagccatgag cttctcctcc tgacacaagt
     4681 tctgctaaat cgtcaaaaaa tgccggataa gagattgaaa cagcttcagc tccttctagt
     4741 tcgcaaggtc catcagctat caaagcagct acttgcagca tcataccgat tcgatgatcg
     4801 cctctgctgg acacttttgc gccatgcaga ggtgtcggcc catggatgat cagaccgtct
     4861 gctgtcgctt ttatctttgc tcccattttc tgtaattctt ctgccaccgc atcaattctg
     4921 ttggtttctt ttacttttaa ttcttctgcg tctttgatga tcgtgattcc ttctgcttga
     4981 gtagccacta aagctagaat gggcaattca tcaatcagac gcggaatgat ttctccacca
     5041 atcgttgctt tcttcaaatg actcgtcttg acacttaaat cagccgattg gttctgttca
     5101 ttgtgattcg tctgggtaat cgctgcgccc atctcctcca acacatctaa gataccagtt
     5161 cttgttggat tgaccccgac attttttaac aacagttggc tttccggaac aagaagtcct
     5221 gctgctagaa agaaagcagc tgatgaaata tcacctggaa cagttatctg ttgaccagtt
     5281 aatttttgcg gtccggtcac tatgatcgtt ttatcttcca ctgtgatcct tccgccaaat
     5341 tgacgaatca tttcttccgt atggttacgg gaacgttctt tttcaacgat tacggatgtg
     5401 ccttctgctt gtaatgccgc aaacagcaaa gcagatttga cttgcgcact ggctacaggc
     5461 atattgtatc ggataggtga taaagagcgg gttcctgtca cagtgattgg tagatactgg
     5521 tcattttctt ctccttgcaa gtcagcgccc atctcgcgta aaggttccat cactcgcccc
     5581 attggacgtt tagacaacga agcatccccg aacagagtag tcgtaaaagg ctgtccagcc
     5641 aaaataccca tcaataaacg tatggtcgta ccggagtttc ccatgtcaat tgattcggtt
     5701 ggttggacaa atgagtggct accgcgacca tgaatcttga tcacctcttc ctcttcttcg
     5761 atctcgacac ctaattgttg gaaggcatgt aaagtactca gacaatcttc tgcgcgcaaa
     5821 aaattttgta tagtcgtcgt tccttcagca attgctccaa acatgatgct gcgatgggaa
     5881 attgatttat ctgctggtat cctaacagtc cctcttaatc catgtatttg ttgtaataat
     5941 tgcatttatc cagcctccta tttgatccag caatgataac tggtattaga cataatgatt
     6001 ctttttgctt gtaacaaatc ctgttggttt ttaaaactga tttgcagtat tcctaaaata
     6061 tcttccctgg tctcttggat cttcaaatta atcaaagaaa ctcccgcttt tccgatcagt
     6121 ccagttactt ctgcaataac gccaggttca tctggcacat cgataaataa atcaaaaaag
     6181 gcaggaatcg cgccttgctt atggacaggc aactggtctc gactttcttt tgcccgattg
     6241 aaaaaagaaa agatttccga ttggttctca gtcatgatcc attctgctat ttgtgtcatt
     6301 tcttgttgcc aatctgccaa tcgttccaat aatatctgct tattgctcaa caaaatatct
     6361 gtccacatga caggatctga ggaagcgatc ctagtgatat ctcgaaagcc tcctgctgcc
     6421 agttgtttgg ctcgcggata ctcttgattg aattgttctg cctgattgac taagccagct
     6481 gccagaatat gtggaaaatg acttagcatt cctgttatac ggtcatgttc tcttgctgta
     6541 agagttacat attttgcttt ggttccgcaa aatagttctt gcagtttttc aacatctttt
     6601 ttcgtctttt ccaaaggtgt aaagatgtaa taggcatttt caaatagttt agcatctgct
     6661 gcctgtatac ctgatttatg agaaccagcc attggatgtc caccgacaaa ccgaagattt
     6721 ttctgctcag cggatgtcac gacttgctgc ttcgtactgc tgacatccgt aatgatgacg
     6781 tgatcagaca agttgattct ttcaaggaca tcaagataag ctaaggttgt acggacggga
     6841 acggctaata agataacgtc tgcttgttct gcacctgttt caaaatcact tgcaacgtaa
     6901 tcaacgatct gctgttctac tgcttgctca cgggtttttg actgactatc ccaaccaact
     6961 actatcagtt ctggattggt ctgacgaata caaagtgcca tagaagcacc aatcagtcca
     7021 agccccacga taaaaacatg ttgtgccatc ttatttgccc cctttttagt aatgtttcaa
     7081 gtagtcacgg tactccttca ctgcagcttt caactcaaca agcgaatcac ttgggaattt
     7141 tgacaatagc tctgtcgcta ccacagtggc tacaacattt tccgcaacga cgctagctgc
     7201 aggaactgct gtactatccg aacgttctac acttgctttg taaggttctt ttgtatcgat
     7261 atcaacactt tgaagtggct tgtacaatgt aggaatgggt ttcatgaccc cgcgaatgac
     7321 aagtggttcg ccattggtca ttccgccttc gaagccgcct agattattgg tacgtcggaa
     7381 atagcctctt tcttcttccc ataggatctc atccatcact tctgagcctg gtaatgaacc
     7441 agaagtgaat ccgaccccaa attctacgcc tttaaaggca ttgatactaa ccacagcttg
     7501 tgcgattttg gcatctagct tcgtgtccca ttgtacgtag cttcctaatc cagctggaac
     7561 gcctccaacg agcacttcta caattcctcc gatcgtatct ccatttttct ttgtctgatc
     7621 gatcaaatct tttattcttt gctcaactgc tggatcaaca actcgtactt ctgacgcttc
     7681 agaaatttct ttgatttctt gaatcgagat atttccaggt atttctgctt ggattccacc
     7741 aagacaaatc acgtgtcctg ctacgatgat gtcaagttct tccagaattt tcttggcaat
     7801 tgcgccgatt gctacacgca tggtcgtctc tcttgcagag gaccgttcaa gcacattccg
     7861 aagatctcga tgatggtatt tcataccgcc taccaaatcc gcgtgtcctg gacgcggacg
     7921 tgcaactcgc cgcagtgatt ggtctttttc aggcacttct gctacagaca tgacagttgt
     7981 ccaatttttc caatctttat tttctaccac taaagtgaca ggtgaaccaa ttgttttgcc
     8041 gtgacggatg ccagacgtga tccgaacacg atctttttcg atcttcatcc ttcctcctct
     8101 gccgtaacca gcttgtctgc gagcgagttc gtgattgatt tcttcttctg acagaggtaa
     8161 tcctgctggt agtccttcaa taatggctgt taattctggt ccatgggatt ctcccgcagt
     8221 catataacgc atcttacagt cctcctaagt aatctttgaa tgtctctgtg ggaacaggaa
     8281 cgatttttgc ttgcccaatc ttttctagca agatgatatt caattggctt cctcttgctt
     8341 tcttatcatg ggttatcgcg tggaataatt catctggatg acttagatcg tacgtaatcg
     8401 gcagatggaa tttttgaagt atcctttcta aagcagctgt cgatccagtt ggagacaagc
     8461 cgattttttc tgcatgagaa gtgatggcta ccattccaat agctactcct tctccatgac
     8521 tgactacccc atatcctgct gtattttcaa ttgcatggcc aattgtatga ccaaaattta
     8581 aaataagtcg atttccttgg tcaaagacat cttcttccac tacttttcgt ttgatttcca
     8641 acgtagcagt aatgattgga acagcatgtt ccagaagatc tttttcgtct ttcagctcgt
     8701 tcagctgttt ccaaagcgtt gcatctgcta tagctgctga tttgatgatt tcagcgattc
     8761 cttctcgtaa acgacgtacc ggcaaagttt ttagaacctc aggatcaatc aatacaccag
     8821 ctggctggct aaatgttccg acaaggtttt tcgctttgtt tgtattgact gctgtcttcc
     8881 cgccaatact agaatctact tgtgcaagta gcgtagtcgg aatttgaacg aaatggatcc
     8941 cacgcatgaa cgtcgaggcc acgaatgctg ctagatcacc caccattcct ccaccaagtg
     9001 caagtatccc atcgctgcgg gtaaaatcat tatctgctaa aaaatcgtat aactcagctg
     9061 cttgttctaa agatttactg gtctcacccg ccggtacgat tgaaaggact ggttcaaaat
     9121 tagcttgtct caattggaac atgatttttt ctgcatatag tggagcaacg tttgtatcag
     9181 tgatcacagc tactttttga ggtctccaaa tcgtttgaag ccatgatcca gcctgttcta
     9241 gtgcgccttt ttcaatatga atatcgtatt tttggttatc aacatgtaca gtaagcattc
     9301 gtttcacttc tttcttttaa aaagctggaa ttaatcgagt gtctgaatct tttccattgc
     9361 ttctttcatg atcgcaactt ctttcatcat gcgtgagtaa gtcttttcat tcaacgattg
     9421 gggaccgtct gaccaagcat ttgcaggatc aggatggatc tctacgatca tgccatctgc
     9481 tccgcttgcc acacccgcac gagccattgg tggtacaagt tcccaaattc ctgtaccatg
     9541 acttggatca acaatcacag ggaaatgagt taattttttc atcaatggga cagcacttaa
     9601 atcaaacgta ttacgtgtcg ctgtttcgta agtccgaatc ccacgttcga taaaaatgac
     9661 aggagatttg tcttcgactg caatgtattc tgcagcatta agccattcat tgatcgtacc
     9721 ggaaattcca cgtttcaatc caacaggttt acctgtttta ccgacagcag atagcagttt
     9781 aaaattttgc atattacgtg cgccaatctg taggatatcg ctgtactcag ccaccatatc
     9841 gatatgccct tcatccatca cttcagtaat cactttcatt ccaaattcat cagccgcttg
     9901 acggatgtat ttcaatcctt cttcttctaa accttggaaa gcatatggag aagtacgtgg
     9961 tttgaaggca ccacctcgca gaatttttgc gcccccggct ttggccattc gcgcacattc
    10021 tctgatttgg tctaatcctt cgatagaaca aggaccagcc atcgtcacga aactgccgtc
    10081 accaattttt actccgtcta catccacgac tgtattgcca ggatggaatt ctcgagaagt
    10141 caatttgtat gtgtttaaaa tacgcacagc attttctact ccttcatagc gtgtaaatgc
    10201 tccttcttgt aaacttcgcg tatcaccttt tagtccaatc actactcgtt ctgttccttg
    10261 atcgattgag actttgaatc cttctttttc gatatgatcg acgacttttt ttatttgttc
    10321 ttccgttgct tcttttttca taattactat cattgttttt cccgctttct tttgtttact
    10381 tatctatctt ggctgatttt ctctactttt tcaaggtggt tttacattaa ttcctactaa
    10441 atgaccgtgt cctgttgttc tcattttcgc tccttctttc ctacttatga cccagataat
    10501 caacaaaaaa agatgtccct gtaggaaatt gcctctacag tgaacatctt caacgagcct
    10561 taaaaagcgc tgcaaaaata taaaatagcc actatagatg aatttctcta cgtggctgta
    10621 agtcgtgttt aaaaatcttt agccatcata gatacaaacc tggtttgttt gtacccacga
    10681 agtcatgaat acaaacctat ctaaaatagc taaagtaata cgtattcagt tttgtaagtt
    10741 ttctagattt cataaaaaat cgctccaaac tgatgtaaga attgtaccta gtatagacat
    10801 aaaaaaatcg actgtcaatc atttttatga aacttttttt aattttctga tagttaactt
    10861 tgtttttcag aaaaataaca attgatttta ttgaagaaaa tagtcatgaa caaaagtatc
    10921 tgagaacttt tttactctct tgttcatgac atatagaata aagcagcttt gtcttgtaaa
    10981 ctccgttttt caaataataa tctaaagatt gctactgaat aataaagccg atttcatggt
    11041 cctcttcgct gctgattcgg taaggtgact caacatccgc tccccagcta ttgatacccc
    11101 cgactcctct tacttttgca aaaatcgtaa gaaccgttcg tctttctggc ggtagttctt
    11161 ctatatgtgt tgcctgttcc agttcttctg ctttgaatgg taaagcggaa aaagcaaatg
    11221 gcgtatcctg ctgttcaaat cgcaacgaaa aatcttcttg atctttatcc gtattatcca
    11281 acgtttgatt tcttgttagt tctacccatt tcgtttccat atgcatgccg cattcttggg
    11341 gaacaaggta tggggaaacg gggagtcctg ccactttata aataccttcg acaccaccag
    11401 ccatccgatc cggatatgtt tcccctgata ggccttcata ctcaaaaaat tttgctttcg
    11461 ttggcataat gaaccttaaa cccaatgcgg gtaattccgg taatcctttt tgtccataat
    11521 aatgcatatt gacaagcatt ttaccctctc cagcgatttg gtaggaaatt gtcacttggg
    11581 tttttggtac tgtgttgatt tccagtgtaa aaatgatttc aaccgtttca gcgtattcat
    11641 tacttgaata ttgattattt actggagcaa tcggcaaatc gatcttttta ttgtccacca
    11701 gtacttctat ttctttgact tttggataca atccggctcc taaccacata ccggatttaa
    11761 atccaaatcc gctacctctg tcattatcgg tcgttgctcg ccaaaaaact ggaagtgttt
    11821 cccggtatag ccattctttt ccgtttttat tgagcgattc cagtccgcct cttgtataag
    11881 aaaaaatata atgcgtattc cccgatttga cacctaatgt acagtcgccg aagacgattg
    11941 caactttttc cgtatccata tgacttttat ttactgtatt tttcataata atatcttacc
    12001 tcctgcatgg cgggtttttc ttgtccatct gcaaaaacga tcccattccc tgagaattca
    12061 tagtcacttg gtttatcgtc aaagtcaccg ccatagcgca tcacttctct gcctgtgatt
    12121 ggatcagtca cttggatcgc ctgatcttta aaatcccata tatagcctcc ttggtacatt
    12181 tcataacgat caagcaacgt catgtaagaa tccattccac ctaacgaatt ccccatgtca
    12241 tgcatatatt cacataagat gaacggtttt tccgctttat tttcaaggta gtcaatgatc
    12301 tcttctggtg gcgcatacat gcagcttttt acatctgaaa tcgaatcttc gaatgctgga
    12361 tttggaaaat tcccttcata atggacaagt cgatcggggt ctactttttt gaagtaatcg
    12421 ttcatttgtc gaatgttttc ttccgcataa gattcatttc caagagacca aaaaagaata
    12481 cttggatggt tttttaatgt ttcgaaattc gtttttgctc gatcaagaac agccgcctgc
    12541 cactgaggta ctgcacctgg tacgttgtaa gaaggctcaa atgcgcccat tttttgccat
    12601 gagccatggg attcgaggtt cgtttccgcc atcacatgga ttcctgcttg gtcgcataaa
    12661 taataccaag tgatctggtt ggggtaatga gatgtccgta cgccgttgat ctttgcattt
    12721 tttatgatag ccaaatcttt ttccatatct tctatagtga tggcgcgtcc ttttttcgga
    12781 tgccactcat gtcgatttac cccgttgata atcatccgtt ttccattgaa gtagatgatt
    12841 ttatctttga tttcgatttt acgaaaaccg aaggaatagt caatatactc cgttactctt
    12901 ttttcttcgt ctatcgtctc caattcaagc ttatataaat tgggctgaaa atgcccccac
    12961 agtgccacat tttcaaatcc atcgatgcga atcgtcattt gctcttttaa cggatattgc
    13021 tgttgataga tttccttccc atcaggatct agtatcgcta cattcagctg actgtttttg
    13081 gctacttgct caaaagacaa atctacttca agtttgccat tttgacattt gtcatctaag
    13141 cgaggtttga tccaaagatc ttctaaatgg ttttgtcctc gagcaactaa gaagacatct
    13201 ctgaagatcc caaaaaatcg gaaaaagtcc tgatcttcca agaatgcagc tgtacttctt
    13261 ttgtaaacct ccacagctag aatattttct tcttccaaat aaggtgttaa atcaaattca
    13321 gacggagtga acgaatcttc ggcatacccg ataaaatgac cattcaacca aataaacatt
    13381 gcttgttcta caccagcaaa atgaatcgag atttgttttc cctttagacc ttcttctaat
    13441 cgaaaagtcg tcaaatacga accaaccgga ttgtctttag cagctgaaaa gatcccttcc
    13501 gcctctttct cagggcttgt cgtgaaaggg ggacggcgaa atacatgtcc ctcccacgga
    13561 tacatcgtat tgatgtaatg gatcttgtca taatttgcta attcaatatg actaggaaca
    13621 gtgatttcat caaaagaaca ggcatcaaaa tcttttttat aaaatgtttt tggtcgtacc
    13681 gaaggatttg gcgaataaca aaaacgccat gatccattta aagactgttt aagggaggat
    13741 gtggtttcta cttcttccct tgttgcataa caaacatgat cgctatgtgc atttgattgg
    13801 ttcactctaa aaacttgtgg atcatctaac cattttatat ttgctttcat acttttttcc
    13861 ttctctctca taactttttg aaatgaagca gttctgtttg gaaatcacct tgaaaatcgg
    13921 ggaagtaaag tccaactgtc atcaattcgg cccccgaaaa tatccgcttc gaggtctgtt
    13981 cctgataaag tgcctcatca tctaacccca tcagatatac ttcatggaac gctggttgag
    14041 ctgaagctag tatcctccct aaaaacacaa tagcttcatc ttgtttagga gacacaaaca
    14101 tccatgcggc aatattttct tcaaaaggac tcgccaaacg ataataatgg ccatactgga
    14161 tcaccggacg aatcgtttga taggatataa tttggtcttt tacttgcttt ttttcttcag
    14221 gagacagctt tgtcaaatcc agttcataac caaaaacagc gctcatagct gtttctcctc
    14281 ttgttttcaa cgaagtagat ctgtcggtct gatgatttgg tacagctgat acatgagctg
    14341 tgatggacga aatcggataa gcaaggcttg ttccatattg gattttcata cgctccactg
    14401 catccgtgtt atcacttgtc catgactgcg gcatataata aataaacccg gcatcaaacc
    14461 gtccacctcc accagaacag ccttcccata agatttccgg ataatcagtt gtcaaatgtt
    14521 caagtagttc ataaagcccg agcatataac gatgcgctac ttctccttgt ctctgtgggt
    14581 caagggtaat cgaatagaca tctgaaagcg aacggttcat atcccatttg atgtagtcaa
    14641 gaggaatcgt atctaaaatc tttctcattt gtttctcaat cgtctgccga acatctaacc
    14701 ttgtaaaatc cagtacatgt tgtgatctgg aagcagaagg cattctacct ggctcctgca
    14761 taagaaaatc aggatgtgtt cgatacagtt cagaatcaac cgagatcatt tcaggttcaa
    14821 accataaacc aaatttcaat cctttgctat gagcataatc tgctatttca cgtaatccat
    14881 ttgtcaactt gccttcatac tcgaaccagt ctcctaatga agaattgtcc gcatctcttt
    14941 ttccaaacca gccatcatct aatacgaaca tttcgatgcc tagctccttt gcttcatcaa
    15001 tgatcgcctt gattttttca ctattgaagt caaagtaagt ggcttcccag ttattgacca
    15061 gaatcggccg ttcagcaaat tgatgacgac ctcttgcgat tctttcacgt agcaactcat
    15121 ggtggatctg actcattttg ttcaatccct gacttgagta agaaaggatc atttcaggtg
    15181 tttgaaaaga ctggcctgca ttcaattccc acgaaaaacg atggctgttg atcccaccga
    15241 cgactctcaa ttgattgatc tgatcttttt caagttcgaa agaatggttg ccggaataaa
    15301 caaattgaag tccgatcgct tctccagtat tttctgtcgt atgatgatga actaaagcaa
    15361 taaagttatt catatggtga ctgctcgttc ctcgacggct ttcaaatact tttgttccag
    15421 ataaaacaga ttgccgtgaa atctgtcttt cattgacatg tgctcctggc aaagcaatga
    15481 cttcatcaaa tcgcctagta tgggcaaagt ctagctggaa actagccgct ttttctagtt
    15541 tgattgtttc tccggtttca tttcttatct ttacagatcg tgtgatcact gctctatcag
    15601 taaatatggt atagaacaaa tcgaaataaa tcgctgtttc tctatcctta agtgaaatca
    15661 ccaacgtttg tgcctctgac ttgtccatca cataagtttg aggcaagcct tcgatctctg
    15721 gcttcccatc tgtaatataa tggctgtcat atctcagatc cagtaagtct gatccattct
    15781 tgcgctgaat gatcgatgcg ggcgttcgaa agtccatgct gccatgagaa gagtattctt
    15841 gcggtagtgt atctttcgac agagaccggt caggatttaa gggaacattt cctgaaaatc
    15901 cacgatctct tcggggataa tttttattgt ctttgtactg cttgactggt ttgccaaaat
    15961 aaatatgcgc caatacattg ctttcttcta tctttatcag ataagaaaga aatgtatttc
    16021 gtaaatggaa gactcggctc ttttcatcaa ataagatgaa atttttatct gtttttctat
    16081 gcgtaatatc catcatttca ctcccaacaa aacggaaacc gtttactttt ttttataatt
    16141 cctgtatact gtttactaaa caattttgtc aataaaaatt ttttattttt ttgaggagtg
    16201 tgtaaaaatg gttggaatta gagatgtcgc acgaaaggca aatgtctctc ctgctacagt
    16261 ttcccgtgtg ttgaataacg atcagacgat ccatgtggct cagactactc ggcaacggat
    16321 agtagaggca gctgctgcgt tggattatga catcagtact cgtaaatata tcaaaaagag
    16381 aatgccttcg attggtgtga tctctacgat cggcaaagcg agcgaagaac aggatatcta
    16441 ttacaaacat ttgcgagtag ggatggaaga agaagctcga cgtctgcatt tagggatgaa
    16501 tcgaatctat aatctatctg ataatcctaa acaatgggaa gatctagatc agttaggtgc
    16561 ggtgatcgtt gttgggactg ttacgaaaga atcgattacc gccttgttaa aacaaaacat
    16621 gcaaatagtc gtcgtggata accctgacat ccagcaagag gtcgatatgg tgtatgcaga
    16681 tcttgaacgg atgactaaaa ccgtactgga attgttctta gaaaatggtc ataaaagaat
    16741 agcctatatc gggggatata aagtcgatat ggatgaattc ggtgggaagc agatttctat
    16801 aaatgaaaag aggatgcgtg cctatcagca ttttatgatc gatcatcact tagaaaaata
    16861 tatgtcttat catctaggtg gctggacaga aaaacaaggt gaggtacttg cgactaatct
    16921 attagaaaat gaatcccctc ttcctactgc cattcttgtc gggagcgatc caatggcaat
    16981 tggtgtctat cgggcattga aacaaaaaaa tatccaaatt ggagaagaga ttgttgtagc
    17041 aagctttgat aatatcgaga caatcgcaaa cctctcccct ggcttgacca gtgttcaaat
    17101 caatgcgaaa tcaattgggc aagcagctgt tcgaatggca ttggagcgaa ttgacggtat
    17161 acgggacgaa ccaatcattt tgacttatcc tgcacgattg attgttaggg aaagttttat
    17221 cccaagaaca aaaaggtatt gaaagagagg ttgtgaaaag ccgcttcgtt tcttcatact
    17281 caaaacgaaa cggctttttc acaacctctt acttatactt attcacaaaa tccaatgtct
    17341 gctggtaaca attttgcgat tcttttagcg tgtaatcaaa ttggtattca tgggtaatct
    17401 ctttttttgt atctgtataa aacaaggatt gcacgggtac ctttaattct gtcaaccgat
    17461 tttctaatgc ttgaccttgc tcctggaatg agtatgcatt tccgtcagtt atatacgttg
    17521 gcggaaattc cttagaaaca tggtcagcca cactagcttc tttcaattgc tcactcgttt
    17581 tccaatcttt tgttccaatc aacgaccacg caacggtttt gacgaaaaac ttcataaatc
    17641 gatcatccga atgctgctgt gctgttagct tcaaatcaac aggtccgcaa taagaaatgg
    17701 ttcctttcag actatctttt ggaagtagct gtttgatttt catttgttta gcatacgcat
    17761 cattcgtttg tatagctgca tattgcaatg cgatttgtga acctgcacta tctccaccaa
    17821 aaaataattg ttccatgtct agcaatttgt cgtctttttc ttcagctaat aaatctttga
    17881 tcagttcatt cacttgaaga acttgattag ggtattctga atcaggcgct aattcataat
    17941 tcatcgcaac cactgcgatg ttggcgtctg aaactaattt tgtcgcaaat tctttgacac
    18001 ccgatttatc tccgccgacg aatcctccac catgtaccca aaatagaaca ggaacaggcc
    18061 ccttgctgtt ttttggatag tagatgtcat aagtattctc tttatacttg gactcatacg
    18121 tcaaatctgt ttttttcgcc acgttttttt ctgcttgatc gaacgttttt ttatcagtga
    18181 tttgaacagt atttgaaaac atatggttga tcaagaatgc tccaggtctg ggggaaagct
    18241 ggaaggcaag aaagattgca aacgcgatcc caagaatgat tccgaagaac catccgataa
    18301 atttaagaat tttctttttc ataaggctcc ctcagtagtt agtattaagc attctgatta
    18361 taccatagtg ttatttccta tcaagctctc ctagatgatt taatgatttt ccccaaactt
    18421 cttctgcaat gcttttgaca aaagaaattt tttccattgt tcttcaaaca aaacattttc
    18481 ttcttctgtt gaagcaaatc cacattgagg acttaagcac aaacgatcca aaggcagata
    18541 tcgtgtcgct tcgtagatcc ggtcaatcac ttgctggtgc tcttccattt cgccattttt
    18601 cgttacagtc aaaatctaag acaactaatt tatcttcaga gactttctct aaaagaacaa
    18661 aactcccagc acgttcacta tcgtattcta aataataggc acggacattt tcttgagtaa
    18721 aataatgggt ctgctacgtc gtcatccccc ctgatgccgc caaaatcgaa taataatttc
    18781 cgcgtcaaac atgtatattt agtgttgaat catttgagaa tatgaaaata aaaatctttt
    18841 tgatcaatca gttaatcaaa ttgaaataac gaaaaatctg agacagaatg acgttcattc
    18901 tgacccagat ttgattttgc acaactcaac agtccttatt ggcaaatcgc caaaataatg
    18961 tagactaact aaaaagacta agaaacaata acaaaagtta acacttctta gtctttcttt
    19021 agcacgtcta tttattaaag attttagggc cttttctcat gtttttacga tcattcttag
    19081 gtgtttccat ttgtttggat cggcctccac cttgtttctt tttgatcaat tctttcattt
    19141 tttctttttg gtccattttt attctccttt aacgaatgaa gcgactaatt cgatatgatg
    19201 ggtttgcggg aaaagatcta caggctgaac tttcttcacg gcataacctg cttctgcaaa
    19261 taacttagca tctcttgcaa aagttgccgg attacatgag atatagacga ttcgttctgg
    19321 tgacatttcg attgctgatt caataaaatg atgatctaat ccttttcgtg gagggtcaac
    19381 tacgatcaca gatggtttga tccctttttc ttgccatttt ttcataactt tttctgcttt
    19441 tccgacttca taatgaacat tttcgatttt attatttaaa gcattgaact gtgcatcttc
    19501 aattgcttct ggaataactt ccatcccata caccttatcc actctgtctg ctaaagacaa
    19561 gccaatcgta cctattcctg aataagcgtc gatcacaatg tcttttttat ctaactcggc
    19621 aaactcgatg gctgtttggt acagatactc tgcttgttga gtatttactt gataaaatga
    19681 ctttgctgag attcgatagg tttttcctaa tagctgatct tcgatatagt ctctgccata
    19741 taaaactttt tctttgtctc ctaaaatcac atttgttttt tcttcattga tattttggat
    19801 gaccgaaacc acttctggca attcttcatg aatagcttca gcaatttcct tggatttaaa
    19861 gaatttttct ttacgagtaa ctaaaacgac catcatttca tgggaataat gaccacgacg
    19921 gataatgatg tgacgaagct gtccttcatt tttttcttca ttatatcctc gtacttggaa
    19981 ccgttgcaag atatctctga ctactttgat tgcttgatcg atgacaggat cttggatata
    20041 aaaatcttcg atagggacta gatcatggct attctttttg taaaaaccgg tggctaactg
    20101 tccatcgata cggcgaacag ggatctgtgc tttattacgg tagccgaatg gttctgccat
    20161 tccgaccgtt ggaagtacag gaacttctgg catcttagca atcttcgcca ttacatgctc
    20221 tacttgtttt tgtttgaaga caagctgttg ttcatacttc aaatgggcta aaggtgcgat
    20281 acctatgcga agtaatgcat tgttttctac aggctgacga tcaggtgatt gtgtaataaa
    20341 tgtttcgact tttccatatc caaacttatt gcctgctttt actactagta tttctacttc
    20401 ttctccaggg agtgcatttt cgataaataa tggataacca tcgatttttg ctacccccat
    20461 tccttcataa ctcaagtcga cgatttcgac tgtatatcgt tcgtttttct ttactatttg
    20521 ctgttctgtc atattttgct cctttaagcc tatttttcaa tcctactgaa ctctactata
    20581 aatcagctaa ttacacaact aaaaccggca ctgaccttac tacgttcagc accggctttt
    20641 gattaattct tttgattagg tgtttcgtct gctatttttc cgtcaccgtc gatatcttca
    20701 tccgttaaac gttcgacttc ttttacgaat tctttgctga caacctctaa ttcttcttca
    20761 tctgtacctg tgattgcttc atcaggaatc tgatcggtat tggcaaagaa ctcaatgtgt
    20821 tgatgaagat tttggaaatg catcggtgct tccccgccgt attcaccatc taagttgatc
    20881 attactggtc gattgatatc atcttctgtt tcaacgaaca aactgcttgt tttggtatag
    20941 attattcttg gatcatcgac atgtcgaccg ccattcagca tgagtgctgc aatatgcaag
    21001 atctcaaaga tatttgctgt cttcacaatg attaaagaaa acttcccatc atccaatttg
    21061 gcatctggcg cgatctgttc gaatcctcca acagagttcg tcagccctag gaaaaacatc
    21121 gaagcatttc cgatatattc cccttcatca tacttcaagc gcattttgat tggctttact
    21181 cgagggagca tctctgctcc tttcgcaaga taagcaagat aaccaaaaat actctttagt
    21241 tctgatggaa cttcataagt caattctgtc aaatagccac ctgcagcgat attgatgaag
    21301 taactgttat cagaaaggcc gatgtccatt tttacggttt ggtgtttttt tatcacttct
    21361 gctgctgctt taatattatc tctaggaatt tttaatgctc gagcatagtc gttcgttgta
    21421 ccagctggga tgatggccat tttaggacgg tgttcaagcg gagcgattcc attaaccact
    21481 tcgttgattg tgccatcgcc accagctgcc actactagat caaaacctag ttctgcgact
    21541 ctacatgctt cattcttggc tgaatctggt tctggtgtag ttgcataggc gctggcttca
    21601 aatcctgctt cttctaatgc ctgtaggata tccgccaaat tttttttcaa taattctttt
    21661 cccgaggtgg ggttataaat tacgcgtgct tttttcattt tcagcctcta tcgtttcgct
    21721 aattcatctt taagtagctt attcacgaca ccaggatttg cttgtccttt agttgctttc
    21781 atgatttgac cgactaggaa gccaaccgca cgatcttttc catttttgaa atcatcgaca
    21841 gattgttggt tgttatccaa aatttcatta atgattggca gtagtttgtt cggatcagat
    21901 aattgcaccc aaccattttt ttcaactact tcttgggctt ctccgccatt tgtgatcaat
    21961 tcacggaaga cttttttggc gatttttgag ctgatcgttc catcggcgat caaacgaatc
    22021 attcctgcta agttctcagg tgtcaatttt gtctcagcta attccagttt ctcactgttc
    22081 aagtaagcag aaacttcacc catcaaccag ttagatgctt gcttggcatc tgcaccattt
    22141 tccaaaacag cttcaaaaaa atcagacatt tcttttgtaa gtgtcaatac cattgcgtca
    22201 tactctggaa gtccaaactc acggatatag cgttcgcgac gagaagctgg catctcaggc
    22261 aaactagctt ttactttttg aatccattcg tcatcgatca cgaatttcgg gatatcaggc
    22321 tctgggaagt aacgataatc actagaccct tctttgacac gcatcaaaac tgttttattc
    22381 gttgtttcgt caaaacggcg tgtttcttgt tggatttctc ctcctgaaag aagaactttt
    22441 gcttgacgtt tttcttcaaa agctaaacct tttttcacga aagataaaga gttcaagttt
    22501 ttcaattctg ctttcgtacc gaactcttct tgtccataag ggcgaagaga aatattggca
    22561 tcacagcgca tagagccttc ttccattttc acatcgctga cttctgtaaa ttggataatt
    22621 gatcgcaatg cttctagata agcgtatgct tcttctggtg aacgcatatc agcttcagaa
    22681 acgatttcga tcaaaggcgt gccttgtcgg ttcaaatcaa cgtaagaata accgccaata
    22741 ccatgcatgt tttttccagc atcttcttct aaatggacac gttcgatccg gatttttttc
    22801 tttttacctt ctacttcgat ttcgatccat ccgtcatgac cgattggttg atcaaattgg
    22861 gaaatttgat atgcttttgg attatctggg taaaagtaat ttttccggtc aaaatgagta
    22921 tctttagaga tttcacagtt taatgccaaa gctgctttca tcccgaattc cagcgcagct
    22981 ttattcatta ctggcagcac gcctggataa ccccagtcga tcacgtttgt attgctgttt
    23041 ggttctgcac caaaatgagc tggcgctgga gagaaaattt ttgagttggt ttttaactcg
    23101 acgtggactt ctagtccaat gactgtttca aaattcattc gtctttccct cctaaaatag
    23161 ctggttgttg tttatggaat tctgttgctt gttcaaacgc atatgccgct tgatacatcg
    23221 ttttttcatc aaaataatta ccgatgatct gcaatccaac tggtaatcct tctgataaac
    23281 ctgccggtac agacatacct ggaagtccag ccaagttgac agggatcgtt aagatgtcac
    23341 tcatgtacat tgtgattgga tcgttaatgt tctcaccgat tccgaatgca actgttggtg
    23401 aagatggccc gatgatcaag tcataatctg caaagacttt ttcaaaatct cgtttgatca
    23461 atgtacggac ttgtcctgct tttttgaagt aagcgtcata ataacccgcg cttaaggaga
    23521 atgtacctaa catgatacgg cgttttactt cttcaccgaa tccttctgaa cgtgagttga
    23581 catatacatc atctaatgat ttcacatttt cagagcgata accgtagcgg ataccatcga
    23641 atcgttgcaa attggaactt gcttctgatg aggcaatgat ataataaaca gccacaccgt
    23701 attttgaatg aggcaagctg acttcttcca caacagcacc taatgaacgg aatgtttctg
    23761 ctgctttcaa tacagcttct tttacgccag gggccacacc ttcgcctaag tattctttag
    23821 gtaaagcaat cttcattcct ttgatgtcgc ctgtcagtcc ctctgcaaaa tcaggaactg
    23881 aaacgcctga agaagtaccg tctttttcgt cataaccgct gatagctgtc aatgctaatg
    23941 cattatcttt caccgtacgg gtcattggtc cgatttggtc aagagatgaa gcaaaggcaa
    24001 tcaaaccaaa acgtgagaca cggccataag taggtttcat ccctacgatg ccgttaaaag
    24061 ctgctggttg gcggatactt cccccagtat cactacctaa agaaactgga atttgtccag
    24121 ctgcaactgc tgctgcagaa ccaccagaag agccaccagg tacttttgtt gggtcccaag
    24181 cattttttgt tttcttgaaa taagaagttt ctgtacttcc acccatagca aattcatcca
    24241 tgtttagctt accaacaggg atcatatctg cttgatagat tttatccatg accgttgcat
    24301 cataaattgg ttcaaaattg taaaggatct tagatgcagc ggttgtcaag atatcttttg
    24361 ttacgatatt atccttgata ccaattggaa taccagcaag gacgttgctt tcatcaatcc
    24421 ctttttcatc taaagctttt gcctgttcca atgctttttc ttcgttcaat gtaatgaatg
    24481 cctcgacctc aggctctgtt tctttgattc gttcaaatgt tgtttttgtc aaatcttgta
    24541 cagtgatttc tttcgataca agcatatgat gcagttcttc tagagaacga ttgtgtaatt
    24601 ctgtcattat gcgccagcct ccccattatc gatgattgcg ggtactttga taaagccgtc
    24661 ttctgtttcc ggcacatttt tcaataattc atcgcggttc catccagctt gcgcccgatc
    24721 ttgtcgcatc acgtttactg tttcaaccac attcgacgta aatggaacac cttccgtatc
    24781 tacttcttcc agcatttcaa ccatatcgat gattttacct aattggtcgg taaaacttgc
    24841 aagttcttca tctgcgaacg caagttttgc taatttggca acgtgtttca cttgttcttc
    24901 actaatcgcc atactacccc ttctttctaa acgagttaaa aactttgctc tctattttaa
    24961 cacaagagtg ccgtgtctga aattgtttat tgaaatttgt ctgaaaaaac tcatttttca
    25021 ttcttctact gagcgacttt tagccaatgc atctgccatt tgatcttctg tccaaacttc
    25081 gattcctaat tcttgtgctt tggtcagctt gctgccagca tcttctcctg ctaccacgat
    25141 atccgttttt ttcgatacgc taccagtcac tttcccgcca agattttcga tcgtctcttt
    25201 tgcttcttca cgagtaaatc gtgttagttt accagtaagg actactgttt tttctttaaa
    25261 aggagactcg acttcttgca attgggcatt tcgaagccct ttgtattcga aattgacgcc
    25321 tgcttgtttt aattcattca tcaattcatg gacttcttcg ttactgaaat aagtgactac
    25381 actgtcggca atgatatcac cgatcgtatc aagcgcaata atcgactcat agtcactttt
    25441 actcaatgtt tctagatcac cgaaatgttc tgctaaaatt tttgctgctt tcgctccgac
    25501 atgtcggatt cctaacccga aaatcaaccg ctcgaccgaa ttatctttgc tgttgtcgat
    25561 tgctgtcaaa atattatttg ctgatttttc ttttattttg tccaacgtca ataactcttc
    25621 ttctgtcaat ttgtaaagat cagcaacatc tgctactagt tttttatcat acatctgttc
    25681 tagtacgcgc ggccccaacc catcgatatt catcgcattt cttgaaacaa agtggttcaa
    25741 accttctttc atttgagcag ggcatttagg attgatgcac cgtaaggcta cttcttcatc
    25801 caaatggaca agttcacttc cacaaactgg acaatgtgta ggcaattggt attcttcgct
    25861 gtctttaggt cgtttatcta acacgacttg cgaaacttct gggatgatgt ctcccgcttt
    25921 ataaatcaga actgtatctt tcaagcggat atccttcaac ttaatataat caccgttatg
    25981 caggctggca cggcttactg tcgttcctgc tactcggact ggtgtcatga tagctgttgg
    26041 cgttactacc cctgttcgac cgactgtcca ttcgatgttt tcgattagcg tttcaacttc
    26101 ttctggcgga aacttgtaag ctgctgccca tctcggtgct ttgatagtaa aaccaagttg
    26161 atcttgtagc gagaattcat tcactttgat gacgattcca tcgatttcat agggcaaatc
    26221 cacacgtttg tcatgatatt cttcaatgta tgaccacact tcatcaatcg aatgacatag
    26281 acgtttttcc cgattcgtgt ggaatccaat tttttctagc tcttctagcg catcatactg
    26341 ggtttttgct tgcataggac cgaaatcagc tactgtgtat aaaaaagtgt ccagatttct
    26401 tttcgctgtg attttagaat ctaactgacg caaagaacca gctgcggcgt tccgaggatt
    26461 cgcaaagata tcttttccct ctgcttctct gtcttggttc aattggacaa atgaccgttt
    26521 tggcatgaaa cattcgcctc ttacttcgat gttcattggt tcttttaatt tgattgggac
    26581 cgaccggaca gtttttaggt tttcagtgat gttttctcct acagttccat ccccgcgagt
    26641 cgcacctctc acgaaatttc catcttcata acgcaaagaa acggaaagtc catctatttt
    26701 tagttcacag caatagtcaa ctacacgtcc cactgctttt tgtacacgtt gatcaaaagc
    26761 tatcagctct tctttgctga aaacgtcgtt caaactgtaa agcggaatat catgagtgac
    26821 tttttcaaaa ccttctaata cttttccgcc tactcgttga gtgggagaat cagacgtgat
    26881 caaatcagga tattgttcct cgattgctac taattctgcg tattctttat cataaacata
    26941 gtcttccact gttggtttat cttctacgta gtattcgcga gaccattggt tcagtcttgt
    27001 acgaagttct tctgctcgtt tagcagcttc gtcaatcgtc atttcatgtt ccatcattta
    27061 ccctccacta ctaagctttt tcaattggcg caaaggcagc caaaagacgt tttactcctt
    27121 gacttgggaa agccacatct agttctagat ccttcgacgt cccgcttact cggacgactg
    27181 ttcctagacc ccattttttg tgtttgactt tatctccagg tttccagctg ttattttccc
    27241 ctccagtagc acttttagag ggaacaaccg ctgtctctgg ctgtttatag ctatatgctg
    27301 tttttttagc gaatacaggc gtttttttag gcgtaggacg catgccttcg atttccagta
    27361 actcctgatc gatttcttct acaaaacgag aaggtcggtt atattgcgtc cgtccatata
    27421 atgttctgga gaaagcattt gttaaataca acgcttcttc tgctcgcgtg atccccacat
    27481 atgcgagacg gcgttcttcc tccagttcgc tttcttccat caatgctcga gataaaggaa
    27541 aaaccccctc ttctagacca atcaggaata caactggaaa ttctaatcct ttggctgcat
    27601 gcaaagtcat caatgtcact tgggaagcat cttcttctaa attgtcaatg tcggaaacaa
    27661 gtgccagatc atttaagaag accgttagtt tttcttctgg tgcatcagcg tcttcttcat
    27721 tttgctgttc gaattgtttg tcgaattctt gtgtgaccgt taaaaattcc tccaagtttt
    27781 cgatgcgtgc ttgtgcttct aatgtatttt ggatttttaa gtcttctaga tatccgctac
    27841 gatccaaaac ctctttcgtc aattctgtca ctgtcaagta tgggatcatc tgagtgactt
    27901 cttggatcat ctctccaaat gcacctaatt gctgtcctgc ttttccaccg atatttgcca
    27961 gatccacatt ttgcgctgct tcaagcagtg gccattcatg aagagaagca aaactgcgta
    28021 gtttttcgat agatcctggt ccgatgcctc gtttgggcga attaacgata cgctcaaaac
    28081 tgattgaatc ttgaggattt accagcacat tcaaatacgc taaaatatct ttgatttctt
    28141 tacgatcgta gaacttgtgc cctccaacca ttttataagg aatatttgct ttcaacagag
    28201 tttcttccat tacacgagat tgtgcgttcg tccggtacaa gatggcaaag tcgccgtaat
    28261 tccgatgatt gcttcgaatc tcttcttgca tccggtctac gataaaacgg gtttcgtctc
    28321 gttcattatc tgcacggtag taagtgatct tgtttccttc tttgttttct gtccacaagt
    28381 tttttggttt tcgattgcta ttgttttcaa tgacttggtt tgctgcgcta aggatgtttt
    28441 tcgtggagcg atagttttgt tctagcaaaa ttactgcagc atctggataa tctttttcaa
    28501 aatccaagat gttctgcata tctgcaccgc gccagccgta aatactttga tctgcatctc
    28561 ccacgacgca caggttccta aatctacctg ccaataaatt gactagcgta tactgtgcgt
    28621 gatttgtatc ttggtactca tctacatgaa tataatggaa tttgttttga taatacgtga
    28681 gtgaatcagg atgttcttca aataatcgaa tcgtattcat gatcaggtcg tcaaagtcca
    28741 tacattgatt atattgaagc tctttttggt aagcagcata acattttgcc gcaatctctt
    28801 cgtaataact gccttgcatt tttgcaaaat cctgaggggt ctgcaggctg ttttttgctt
    28861 gagagattgt tcctaatata cttcgaggat catatttttt cggatcaatg ttcagttctt
    28921 ttagtatccg tttcatcaag gtcagctgtt cagaagaatc gatgattgta aagttccggt
    28981 tatacccgat aaaatcaaca tctcttctta aaatacgcac acacatcgag tgaaaagtcg
    29041 atacccagac atcttcgcct ccagatgcga gaatcgcatt gacacgttct ttcatttctt
    29101 ttgcagcctt attggtaaaa gtaatcgcta ggatgttcca aggattgact tctttttcct
    29161 caatcaaata ggcaatgcgg tgtgtcagca ctctcgtctt tccacttcct gcacctgcca
    29221 ttagcagtaa tggtccgtca gtgtgaagaa cagcttcttt ctgacggggg ttcatcccgt
    29281 ttaataattc agctttggat gacattaaca acatcctttc ttttatcaat cttgttaatt
    29341 atagcatttt cttggctttc attaaactag ctagttcaaa atcatttgtt tctttttatg
    29401 gatttttcct tgtcaattcg ttgatttcgc gctaaattaa aagaaagcga ggcgtttgaa
    29461 tggaaaaaaa aggaaaaatc aagaaattag ataagactca gatatatttt attctaaaag
    29521 gaatatttgt tggactgctg acaggaatcg tcgttagcct gtttcgatta tctatcgaaa
    29581 agctaagcga tttagttcga agtatctatg aaatgagtcg ggaacagccg atttacttaa
    29641 ttggcctagc tatttgctgc atccttgcag catttttcgt gggttatctg gtcaaaaatg
    29701 aaccggatat caagggaagc gggattccac aagtagaggg acagcttcgc ggggaattat
    29761 caatgaactg gttctctgtt ttatggaaaa agttcattgg cggtgtactt tcagttggtg
    29821 cgggcctttt tctaggtaga gaaggacctt ctatacagct tggcgcatcc attggacaag
    29881 gagctggaca attgtttcgg agtccatctt cagaagaaaa gattttaatt tctagcggtg
    29941 caagtagcgg acttgcagca gcattcaatg ctcctatcgc aggattactc tttgttttag
    30001 aagaaatcca tcactccttt tctccacttg tttggctgac atcatttgct tcagcaatca
    30061 ctgctaactt cgtctctctt tactttttcg gcttgagacc tgttttatat ctaggtgacc
    30121 ttccttcttt gccgttgaaa tattatggtt ccttagtgat cttaggagta cttctaggca
    30181 tcttaggttt tatttatcag aatgtcttgc tggctttgcc aaaattttac aaccgattgc
    30241 ctttaccagc ttattttcac gggttcgttc ctttcttgct gattttgcca attggttatt
    30301 tatggcctag tactctaggc ggaggcaatc aaatcgtttt agcttttgga catgcttcct
    30361 tgccaatcat tatgattatc ggtttgtttg ttcttcgatt cgttttttcc atgatttctt
    30421 acggtggggg tcttccaggg gggattttcc ttccgatatt gacattgggg gctttaatcg
    30481 gtagctttta tggaaacatt atggttgatt tcgggatgga tccaatctac atcaaggatt
    30541 ttgtcgttat tgccatggct ggttatttca cagcgattgg aaaagcaccg ctgacagcaa
    30601 ttattttagt gacagaaatg gttggctctc ttaatcatct gatgccttta ggattggctg
    30661 ctcttgttgc ttatatcgtc aatgaccttc tcggcggtaa tccaatttac gaatcattat
    30721 tggaacgtct acttggaaat actcagaccg cctcactaaa aggtgcaagg acaacttttg
    30781 attttccagt aacggcagag agttctttag acggattaat ggtacgagac ttcaattggc
    30841 ctaaaaatat gctgctaatc tctatacgca gaggaaatca ggaaatcttg actcacggtg
    30901 atacgatcat gaatgtagga gatattttgg ttatcttgac tgatgaagca aacttgccaa
    30961 gaatcaagca ggaaatcgag gctaaatctt ctatcacaaa tttgaagcaa tcattttaac
    31021 gaacatggaa aaactatttt acaacaacta ttcatctaca ctacttgatt ctatatgatt
    31081 ctataaaaaa agaagaggct gggaaaaccc agcctcttct tttttatttc tatgcttctg
    31141 ctactgcagg agcttttctc aataaaccat aaatcacacc agatacgact gctccgatag
    31201 caataaacac caaatacatg accgggtgac tcagtaagaa tacaacgaaa atcccgccgt
    31261 gaggagccag taatttgatt ccgaaagctc cgactaatcc gccagttaaa gcagaaccaa
    31321 taataaaact tggaatggca cgaatcgggt cagctgctgc aaatggaatc gatccttccg
    31381 tgacaaacga tagtcccatc acgatgttcg tcaaaccagc atcttgatct gttttgctga
    31441 atttattctt aaacaaacgt gtagcaacga aaattgccaa tggaggtacc attcctccag
    31501 ccataacaga agccatcacg acacttccgc cagttgcaac agtcgtagct aaagttgctg
    31561 taccaaacac ataagcggct ttgttgattg gaccgcctaa atctgccgcc atcattccag
    31621 ctaataatgc accaagtaaa gcagcattcg aaccactcat acttgtcaag aaaccgttca
    31681 atgcatcatt gattgctttc atagggacat tgaccagcaa catcaagaaa ccagtaatca
    31741 gtaagccaaa cactggataa aagaggatcg ttttaattcc atccaatgat tttggtaagt
    31801 tcttgaataa tttacgtaaa aatacgatga cataaccagc taagaatcca ccaatcagtg
    31861 cacctaagaa tcctgcaccg cctgtattag ctaatgcacc agcagcaaat ccaacgatca
    31921 aaccagggcg gtcaccgata cttgaagcaa taaatcctgc taataccggc agcatgaaac
    31981 caaatgcagc ttgtccgatt tggttgaacc agctagcagc ttgattgtaa ttccctagat
    32041 ttgataattg atcatgtggc acgccaataa attggtcgat catgaaagaa agcgcaatag
    32101 cgattcctcc accaataacg aaaggtaaca tgtgtgatac gccgttcatc aaatctttgt
    32161 aaattctttg tccgatagta ccatccgcat ttccttcttc tgaactgctt tcttttccat
    32221 caccatggaa tactggtgca gtaccactaa gggctagatt gatcaactct tctggtttac
    32281 ggataccatc actgacagga cgattgatca gttcttttcc gtcaaagcga ttcatctcta
    32341 cttttttgtc tgctgcaacg attacacctt ctgcacgggc gatgtcttca gcagtcaatc
    32401 ggtttttgat tccttctgaa ccgttcgttt cgactttgat ctccacaccc atttctttcg
    32461 cttttttctt caaagcatct tctgccatgt aagtgtgagc gatccctgtt ggacaagctg
    32521 taacggcaac aacgaatttt ctttctggat gagcagaagc ttctgcagct tgtgtttctt
    32581 gttctgcttt ttcttctgct tctttttgct cttcagctgt ttggaacaag tcttggactt
    32641 cttctggtgt atttgcttct tttaattttc ctacgaaatc tgcatcaatc agcaatcgag
    32701 atagtgctgc taatgcttgc aaatgtgtgt cgtttgcgcc ttctggagct gcgatcatga
    32761 aaaacagata agttggctgt ccgtctaagg cttcataatc tactcctttt tggctttttg
    32821 cgaataaaac tgttgcttct ttaactgcac tgtttttcgc gtgaggcatg gcgattccat
    32881 caccaagacc tgtagaagtt tgtgcttcgc gggctagaat tccttcttta tatgtttcga
    32941 tatcggaaat ccggccggca tcaaacattt tttggaccat ttcatcgatt gcgccttttt
    33001 tatcagtcgc ttgcaaatcc atgatcatcg cttctttgac cattaaatcc ttgatattca
    33061 tgattatcct ccttgaaact aatcaatttt tgtgatttca acttctggca gcaactcatc
    33121 aatcaattcg cgagctgcca agtcatcaga aaaagctgtt gcgcttccgc aagcaactcc
    33181 ccatttaaag gcttcgattg gatctttcgt acggctgtaa gccccaataa agccagcaat
    33241 cattgaatcc cctgcaccta ctgaattttt caatgggcga actaatacat tcgttttata
    33301 agttccttgt tccgtgaaga gcaaagcacc gtctccagcc attgagatca gcacattctg
    33361 tgccccttca gctagtaatt ttttcccata cggaatgatt tcttctacag attggaaggt
    33421 tgtgttgtaa agctcagcta attcgtgatt gtttggtttg atcaacaacg gtttcttaac
    33481 aagtgcattt tttaggtcat cacctgttgt atcaatcacg aaatctgcac ctttttctcg
    33541 aatgatatca atcagttctt gataaaatcc ttttcgcagg ctagctggcg cgcttcctga
    33601 caatacgacg atatctcctt gtcctactgt tcctaatgct tgcttcaact catcgatttc
    33661 ttcatctgaa atagatgggc ctaagccatt gatctcagtt tcagtgtctg attttagttt
    33721 gatattgatt cttgtatctt cttttacggt tgtgaacaaa gactggattt gttctttttc
    33781 aagccacttc gaaataaatt cgccagtgaa ccctcctaaa aaccctagtg ctgtagattg
    33841 agaatcgatc cgtttcaaaa tacgggaaac attgatccct tttcctccag gtaacttgaa
    33901 gtcttccttc attcggttca aatcgcctag ttttaaccca tctacgcgta cgatataatc
    33961 aattgatgga ttaagtgtta ctgtgtaaat cattgtattg cctccttaat tgttgtcttt
    34021 tcacggaatt cattaacgaa ttctaatgga caacgctcgg ttatgatcgt ggctgcttcc
    34081 aaaggtgcaa cagaggtgaa ggacaactgg ttgaatttgg tatgatcaat caatacaaat
    34141 gtctcttcgg aatttttcat ggctaaacgt ttaacagctg cctcttctgg atccggagtc
    34201 gtgaatcctt gttcaagatg ggcaccgttc attcccataa atgctttatt aaaatgaaaa
    34261 ttttttagtt gttctatcgc attagatcct aaaacagcat tagttgccag ctttatcgtt
    34321 ccacctaaaa taatggtagc aagctccaaa tcaactaaat ttgccgcatg ttgaacggaa
    34381 tttgtcacta ctgtgatctg ttttccttgc agaaaaggaa tcatttctaa cgtagtagag
    34441 ccagcgtcta aataaatgac atcttcgtca ttaatggttt cagctgccga tttggcgatt
    34501 tgctgttttt cgtgaacgtt tttgagtgat ttttcactca tattctgctc aaaattcaat
    34561 aattgctctt tcttagctcc gccatgaact ctttctagaa acccactatt ctccagctct
    34621 tgtaaatcac gtcttatcgt tgattcagaa gcattcaaaa ggtttacaag atcttgtgat
    34681 ttcacgattt tctgctgttc aagcaagtct aggatttttt gtctgcgttc ctctgtaagc
    34741 attttcacga tcacctcaat acaaataata gcatattttt tttctactag caatataaat
    34801 cagtcaaaaa cgttcataaa aattataaat cagtcaaaaa aaaataaaaa accagtcaga
    34861 aagactggtt ttttattttt ttgtctatca tcaagccgtt ttatcgtttt ttattttcgc
    34921 ttcttgtttt tttcatataa acagaagcag gttttctttc cttatgaaga agataataat
    34981 aaagtcccat cccgccaaac atgaccaaaa aaccgatgac tgatcgaata ggaaaattag
    35041 gaacaaaaaa gagacagacg gataataatg atgccggaac cattattcga taatagagtt
    35101 gtttatccat tttcttcacc tcatattttt tgtaaagtaa tcaattgttc taattctcgg
    35161 taatctaaaa attgcacacg cagatgctgc tcacctttct tgacatcgaa ttggatactt
    35221 cgccgctttt tctttattcg tttgaaggta acatcagaga gtctatgttt gatttccttt
    35281 aacggtaatt ctgacaggga ttctgaagga acatagattc ttaaggataa aagatactct
    35341 tgatattttt catctgttac ctctacttct agatattccc taatataata acaataagct
    35401 tcgattccat tatccaaagc aacagtcgga tcgataactt tatagcacat catgcgtgat
    35461 cgattctcgt caacccaagg aaaatacgcc tgagcattta gtgttccgcg aacacgcctt
    35521 tctttataat ctccttgttg gatatcataa ctttttgtta caaaaaatga agcattcttt
    35581 ttaggaacag gttgcatttg tgcattctga tactggacag attggatcgt atggtcagtg
    35641 taaatgtatt caacgaatgg cggtttcaat aaaccgacac cattgtccca agcaagatga
    35701 tcaaacccaa gctggtccct aaaataggat tgaaacattt gattgtttgg cttttgccag
    35761 ttaaaggggt ctcgataaag ctggatacat atgacttcgt tttctaattc cttcactttt
    35821 tctacttgtt ggatgtcgag aaaaatcggt ttagggatga tttgatgata atagcgacta
    35881 taatacattt cccaacacga agtgaaacat aaaccttggt aaaaaagatc gtatcctgga
    35941 aattggttgc aatcgactac tacttccccg ttttgttgtt tcattttagg taaatgttcg
    36001 atttcatcct ttttctcaaa caatagccgt tgagagatct gttttgtatt gtgatagata
    36061 aactcgctat aagctcgcat ataggcgaac accccatgag caatcatttt tttctgaatg
    36121 aagtctcgaa tcaaaaattg cttttctttg aaaacagaga tagacaatac attttttatc
    36181 aataattgat tatcttccaa gcgaaaacta cattcgttct cgtcatttcc tacaactaac
    36241 caactttcgt ctcttaccga tgctagcatt attttttctt gaagctttcg aagatcatac
    36301 ggatccgctt gttgtgtgaa agagcggata aatgttggct ggatcaacag acgtgtcaaa
    36361 atagcccaat cgcttatttc ctgttttttt tcttcttcat ttaagtctgt gagctggata
    36421 gtcatcaata atttatcttc gccaatcatt ttctcacctc tttcaaatct ttttataatt
    36481 taattataac caatgataaa gaaaaactaa aactttatgt cttagaaacc aatttggacg
    36541 attacatgat agactaagta aaaagaaaca aagaggtgtt tacaatgggg ttgaaatttg
    36601 aacgtacaga taatttagat aaacttttcg aatcatttgc tgtcgatccg gaaaaaacag
    36661 ttgcaccaaa agaagagatc gatcgatttc taaaaccaca gccacaaaat gaaaagaaaa
    36721 aagacaagaa acaaaagtaa gttaagctca aaaaaagact taccagccaa acttttatga
    36781 aagagtttga ctggtaagtc ttttttattc tttttatgat tcaattgctt gttcgattgc
    36841 tgcccacgct tcttcttttc cctgttttgt aacggaagaa aagagaataa acgtgtcatt
    36901 gcgatcaaaa tccaacgctt ttttgatttc gctctcatgc ttgttccatt taccacgagg
    36961 gatcttatct gccttagtgg caacaacaat tacagggata tcataatact tcaaaaattg
    37021 atacatctgg atatcttctt gagaaggctt atgacgcaaa tctactaaag aaacgaccgc
    37081 acgtagctgg tctctagtcg ttaaatatgt ctcgatcatc tttccccatt ttgctcgctc
    37141 agttttggat acttttgcat acccatatcc cggaacatcg acaaaatgta atgcgttctc
    37201 gatcaaataa aaattcaatg tctgggtctt tcctggctta ccggaagtac gggctaaatt
    37261 ctttcgatca atcaatgtgt tgataaaaga agatttccct acatttgatc gtcctgccag
    37321 agctatttct ggcaattgtg tatctggata ttgttttggt gccactgcac ttatgataat
    37381 ttcagcttga tggactttca tggtctctct ccttaacaag gtgatgaata aagcaagcgg
    37441 ctgtcgcaaa agccaaaatg acttttacgg cagcctcttt tcatcaaccc gcttttttct
    37501 ctgaatcttt atagattact gttggctcac cagacaattc agctgcttct tttgtgataa
    37561 tcactttttc gatgttttca tttgaaggga tatcaaacat cacatccatc atgatttctt
    37621 caatgatgga acgaagtcct cgagcaccgg tattgcgttc gattgctttt ttcgcaatgg
    37681 ctctcaacgc ttcaggttcg aattcaagtt cggtattatc tagagaaaga agtttttgat
    37741 attgtttgac tagcgcattt ttcggctcag tcaagatccg taccagatca tctgttgtca
    37801 atttctctaa agctgccata acaggtaatc gtccgataaa ctcagggatc aatccgaatt
    37861 ttagtaaatc ttcaggaatg atatgctgca tgacactttc gtcttcagaa agtttttggt
    37921 tttgtgtacc aaatccaatc gtcttttcgc ccatacgatt cttcacaatc gtttcgatgc
    37981 catcaaatgc accacctacg atgaacaata tgttcgtcgt atcaatctga ataaattctt
    38041 gatgtggatg tttacgtccg ccctgaggtg gcacacttgc aacagttcct tctagaattt
    38101 tcagcaaggc ttgttggaca ccttcgccgg aaacgtcacg agtaatagat acattttcgc
    38161 ttttacgcgc aattttatcg atctcatcaa tatagatgat ccctttttct gcaagttcca
    38221 cattgtaatc agcagattga agcaatttaa gcaaaatgtt ttctacatct tcccccacat
    38281 aaccagcttc tgtcaaactt gtcgcatctg cgattgcaaa aggtacattc aatgtttttg
    38341 ctaatgtttg tgctaaaaat gttttaccag accctgtagg tccgatcaag cagatattgc
    38401 ttttttgcag ttcaacatca tctgtgtttt cttgtgcttg ctgattgaca cgtttatagt
    38461 gattgtatac ggcaacagct aacgagcgtt ttgcgcgatc ttgtccaatc acgtagttat
    38521 taaatacttc aagaatttct agtggtttag ggacttcagt gaattcgcgt acagcttctt
    38581 cataaaattc ttcatcaatg atttctttac ataggtcgat acattcatta cagatgtaga
    38641 cccctggacc agctacgatc tttttcactt cttcttgggt tttcccgcaa aaagaacagc
    38701 gtacggtctc attgctactt ggattgtcat acatggtctt caccccttaa cttgcaaaaa
    38761 gtatgcaaga aactatcaaa gactttacag tcttttacta tcataacata gtttagtaaa
    38821 aagaaaaagt gtttaacaaa ggatcaactg acctatacct ttgcattttt tactcttaac
    38881 aaaaaataag gaactgtgac aaaatcgtgt cacagtccct tatttcgaat aaacaatttt
    38941 ctagaagtag cttcttcaga aatgatttat tacttgattt ctattctttc gcttctgtcg
    39001 ctgtacctgt gatcaattcg attgcttttt tcattgtcac atcatgttcc agcatatctt
    39061 ctgtcaacac gcgtttgatt tgttcgattg gcatgttgta tgtttctgat aattcttgga
    39121 tttctgcatt gatttcttct tctgttgctt gaagattttc agcagctgca attgcttcga
    39181 tcacaagatt tgttcttgtg cgcatttctg cttcaccttc gaattgttta tgcaagtctt
    39241 cttctgtaga accagttaat tgatagtaca tttcaggaga gataccttga cgttgcatgt
    39301 tgttcaagaa ttcgtccatt gaacgatgta cttcatcatg aaccatcacg tgtggcaatt
    39361 caacgatttc tgcgttttca acagccatac ggatcgctgc ttcatctttt gcatcgtccg
    39421 ctgctttttc ttttgtttct actaattctt tgcgatattt ttctttcaat tcgtctaatg
    39481 ttgatacttc gtcatcaaca tcttttgcga attcgtcatc taattcaggc acttcttttg
    39541 cttttacttc atggattgtc actttgaaga ctgcttcttt tccagcaaga tcttctgctt
    39601 gataatcttc tgggaaagtt acatttacat ctagactttc acctgattta tgaccaacta
    39661 attgatcttc aaaacctgga atgaatgagc ctgaacctaa ttcaagtgag taattttcgc
    39721 cttttccgcc atcaaacgct tcgccatcta caaagccttc gaagtcgatt actactgtat
    39781 cgccattttc agctgcagca tcttctttga taactaattc agcttgtgct tcttgttcac
    39841 gtttgatacg tgcatccacg tcagcatctg ttacttcacg gtcttgtttt tcaacaacta
    39901 gatctttata ttgtcctaat tttacttctg gtttcactgt tacttcagca gtgattaccc
    39961 agtcttgacc tttttccatg ctttcaacat cgatttttgg ttgagaaaca ggatcgatgc
    40021 cagcttcttt gacagcagct tcataagctt ctggtaatac agcattcaat gcatcttcat
    40081 ataaagcttc ttcaccgtac atacggttga atacttgacg agaaactttc cctttacgga
    40141 atccaggtac gttcaagctt tttttcactt tattaaacgc agttgttaat cctttttgga
    40201 tttcgtcttg cgcaatcgta aattttaaaa caccatcatt cgtgcctttt ttttcaaatg
    40261 ttgcagtcat ttaattccct ccgagtttag aaattcattt tatacatcaa aaacgtttca
    40321 tgcatacctt taaatagtac actaaccctt tcaaattgta aacatattga atggatagaa
    40381 tccttgtttt tcataacatt tttatctttt tcactgataa aatctaatgg aagaagatca
    40441 tttgctgcaa aattttttct ctttttttta aaaaatcttg tttttcatct tctgtacctt
    40501 catacatcgt ttttcctgta cacgcctcta cagtcagttg aaaccagcgc ttcgggtctt
    40561 cgatgtatgt atcatagaca ggataaacaa ttgcagattg caacgtgaat tcttctttta
    40621 acacggaact aagcagcgca tcttcattcc cgtaacgatc tgaaaataca cataattgct
    40681 ggtaacttgc ctgctgctgc ggctttggca gatcttttgg tatgactttg actaactttt
    40741 cttcaatcgt caaatagaaa acaggatggc attcgttcaa ttgcctaagt gtctccagta
    40801 attttgccct tgctaaaata tggacctctg gcgatacaat aagtttactc gctgtttgga
    40861 taaactcatg ataaggaagc tgttcgatct catcaataga cgcaagctga cgtgcaggtt
    40921 ccaacgaagg aagttcctcc gatttattgt ggagtgcctt gatcttctgc ctctccattt
    40981 gtccggataa ttcttctgca aattggattt tttcaatgac ccgctgctcg aaagcttcat
    41041 aaaaatctgt acttgctatc aattttctag catataaaaa atcttgtgta ctgattaaca
    41101 agtcaaaata aaattcagca aactcctcat tttgaagata ctgtttttca tgcaagagtg
    41161 cttgcttcaa tgcttctttt ttctcgtcca actcgtaaag acaaaaaaca agcaggctat
    41221 tagcctcaaa gctttctttc aactcgtaag ctcgttgaaa taattctttt gcctgctgat
    41281 attgctggtt tgctagtgct tgttgtgcca ctcgcattaa attctcatat ttttctggaa
    41341 aatcaatcgg cttcacgcag tgtcctcctc ttgctcatat tcagctcatg ttcaatagta
    41401 gcaaaacaaa aaaagcatga caattaaaaa taactgaagc accctctaaa agctagattt
    41461 tagatctgac ttttagaagg tgctttcaac tgttactgct ttttattgct catcagctgt
    41521 tgacttcctg gttgaacgct ttgatcagat tgatcgtaac aacacttgta ttagtgaaaa
    41581 aatcgcccag atgttgcttt ttctgattaa agactgctgg aagataccct aacatcagca
    41641 aaggaaattt tagaatgaac cggcaagcac cttctctaac taaaacggtt gaccagctaa
    41701 gttcttcttc atcaaagctg atgactcgaa taccaaagat cattttccca atcgtttggc
    41761 catgattgaa tttcgtcaat aaaataaaat acgctagata aataagtaag gctgtgaatc
    41821 cgtagagact cagataagaa tctgaaaaat taagccaacc tagatttttg gcaataccaa
    41881 gtgttaaacg cgtaatagct ccgatacaca aaagatcgac caagaaggca aacattcgga
    41941 taaaaaaacc tgcatagaaa tacttaggaa aatcatttgc ttttggcggt tcatttcttg
    42001 tgtccgtata ctttttccat ttacgttgtt tctttttgat ttcttcgggc gtcaaaggct
    42061 ctctttcaaa agagctcagt accttttggg aaaagttgga gtcaggtcct acagcaggct
    42121 gctgttgctt atctttttct tgttccttat tttcatttga aagctctgac atgcttactc
    42181 acctccgtaa taatacattg cttttggtga ttccgctgtc cctagattct caagaacgga
    42241 taagatctga ctagtctcag aaggcttcaa tccctggaat tctgctagtt tacttcctaa
    42301 ccatgtacta gcaaacccag atggcgtgga agaatattct actagttccg catcttcaag
    42361 ctgttgttcc tttcgtatag cttttaaagc atcttcagga aatcccaatt catcgactaa
    42421 acctacttct tttgcttgga caccatcata aatccttcca tcagcaattt ttttgacttc
    42481 ttcttcagat ttattccgtc cctcgctgac gattgctaca aaacgactat atgcgctgtc
    42541 gatatatgct tgaagcaccg cttcttcttc ttttgtctct ggtcgcgttc ctgaacccat
    42601 atctttcaac gctccgcttt taactgttgt atcttccaca cccagttttt ctagaagtcc
    42661 tgaataattc aatcctgaca tgatcacacc aattgagccg gtcactgttt cttctgttgc
    42721 aaagatttta tctgcttgag cagaaatata gtagccaccg ctagctgcca tgtttttcat
    42781 gctgacatac attgggatat catgttcttt acgaatcgta tccaatttct tagcgatttc
    42841 cgcactttcg tagacaccgc caccaggaga atttacttct aaaaagatac ctttgactgt
    42901 tggatcttct tcaatcgcct tcagttgttc cataaaattt ttatgattgt aaccttcaga
    42961 agcaaataaa cctgaagacc ctcccgcagc aatcgttcca tctactgata attttacgat
    43021 tttttgcgta ctgtctccct cttccaacac atttggagct agttcttcat ccccatataa
    43081 ccaactgttc aatccctcaa tttgatcgtt ttcctgaggc ttagtcaaac tagctgaaac
    43141 taacgaaacc acaaataatc ctgcagcaat cagaactgct gcccaacgtc ttttattcat
    43201 tctttctcct ttctaataca agttttttat gacgatcaca gtttctgact gctcagaagt
    43261 gtcgaaccgc tctgcttttt cttcccgcgc atacccggaa aacgactctt ttgttaattc
    43321 atcttctacc gtaaactcac gagcttgagc aacttccaag gcatcaggtg cgatttcttg
    43381 gatgctatcc cagccagtat ctaaaaatat tttcgtatca tctgatacac cagctgatgc
    43441 ttcgaatttg gacaattgtt tttttaacat tcccagtgtc atgatctcaa tttttgaatc
    43501 catttttact cctcctctat ccgattactt tctattgtac cgaactcctg aaaaagatga
    43561 tagataaaaa cccattattt tttgacataa aaaggacatt tgacaaagaa tttgatatat
    43621 accaatttat tttttattct ttttttgttg acaagaacat tcgcaaaagg ttatgatttt
    43681 aaatgtttcc agataaaaaa caaaaaggag gatagttata ccttttagga tagcgccagg
    43741 tctgtgtaaa cagatgacga ggagagggtt tatcgaaaga ttcggcggat ggccctaggg
    43801 actgcactct acagaaaatc cacaaaaaat gtctgcaaag ataaaacaaa aggattttcc
    43861 tgcagctgga agcattcata cgaattcatt atagaaagta ggatgttttt tatgtgcgga
    43921 attgttggaa tgattggatt aaaaaatgta acaccaggat tgattgacgg tctagaaaaa
    43981 ttagaatacc gtggttatga ttcagctggt atctttgtct cagatggcac aactgattat
    44041 ctggtcaaag cacaaggcag aattcaaaat ttgaaaaaca aaataaatat tgatactaca
    44101 ggaaatatag gtattggtca tacacgatgg gcaactcacg ggcaaccttc tgaagaaaac
    44161 gcccatccac atacttccca aagtggacgc ttcgtgctcg ttcacaacgg cgtaatcgaa
    44221 aactttgaag aacttaaaat tgcttattta gcaaatgacc actttattgg tgagaccgat
    44281 actgagatta tcgctcatct aattgaaact ttcgcgaagg atactacaac gcaagaggcg
    44341 tttttaaaag ctttgcgggt catcaaaggt tcttacgctt tcgctttgat tgatcggaca
    44401 gcacctgatg ttatttacgt cgctaaaaat aaaagtccac tattaatcgg actcggcgat
    44461 ggattcaatg tgattgccag cgatgcaatg gctatgcttg ctcatacgaa ggaattcgtt
    44521 gagattgaag atgaagagat ggttaccgtc acatcagaaa aagttattat tcaaaatttt
    44581 gcaggtgaca tcgtagaacg ttcttcattc gaagcacaag tagatgcttc agatatcgag
    44641 aaaggtacct atccttttta tatgctgaaa gaaatcgacg aacagcctat tatcatgcgt
    44701 cgtatcgctc aaaaatacgt aacagaagac aatcgtgtct ctcttgatca aaagctggtc
    44761 gatacattgt ctgatagcga tcgaatctat atcgttgctt gcggaactag ttaccatgcg
    44821 gggttagcag gtaaacagac tttggaaaaa ttgacacaga ttcctgttga ggttcattta
    44881 gccagtgaat ttggctataa cacacctctt ctttcaaaaa aaccattctt tattttcttg
    44941 agtcaaagtg gcgaaacagc agacagccgc caagtgctgg taaaaatcaa ccgtctcggc
    45001 tacccttcac ttacgattac aaatgttgct ggttctactc tttcgcggga agcatccttt
    45061 acactgctat tgcatgctgg acccgaaatc gcagtagcat caacgaaagc ttatactgca
    45121 caaatagctg tactaacttt attagccaaa gctatcggtg ataaaaaaga atttgaaact
    45181 tctcttgtat tcgatatcac tcatgaattg agtttagttg ctaatgcgat ggaatcggtc
    45241 atcgcccaaa aagattattt agaagagttg gcagaagtat atctcagcga tacaagaaat
    45301 gccttttaca ttggacgtgg tgctgactac aacgtttctc ttgaagctgc tttgaaattg
    45361 aaagaaatca gttatatcca ggcagaaggt tttgccgctg gcgaattgaa acatggaacg
    45421 attgctttga tcgaagaagg aacaccagtg atcggaatca tcagtgaaga agtgactggt
    45481 gcgcatactc gaggaaacct gaaagaagtc gaaagccgtg gcgctaaaac actagttatt
    45541 gtctcagagg gattagaaaa ggaaagtgac cagctggtgc taccaactgt ccacccttat
    45601 ctaactactc taaccacagt cgtaccgact caattacttg catattacgc aacgctatta
    45661 cggggattcg acgttgacaa accacgtaat ttagcgaaat cagtgacagt agaataataa
    45721 aagaacaagg accagtgtca gacatctgac actggtcctt gttcttttat tttttatgaa
    45781 aatatctctt aatccagtcc gatttcttct ttgactactg ccgcgatttt atcgacatag
    45841 taattaactt tctcatcagt aggcgcttct gccatcactc gaagtaatgg ttctgtaccg
    45901 gaaggacgaa ctaggattct tccttctgat cccatttcag cttctgcctc ttcaatcact
    45961 tttttgattg ccggaacatc catcgcaccg tttttatcac ttacgcggat attcactaat
    46021 ttttgcggat agattgttac ctctgctgca agctctgata atttcttacc tgtctgtttc
    46081 atgatattca gtaactgaat tcctgaaagc atcccatctc cagttgtgtt aaaatctaag
    46141 aaaatcatgt gcccagactg ctcgccgcca aagttataat cgtttttgcg catttcttct
    46201 acaacgtaac gatcaccaac ttgtgtaacg acatctttca aaccgattcc ttccaccgct
    46261 ttgtggaatc ctaagttact catgacagtc gtaacgatcg tatctttttt caatcgattt
    46321 ttttctgcca agtatttagc acagataaac atgattttat caccgtcgac aatgtttcca
    46381 agttcatcca ccgcaataat acgatcgcca tcgccatcga aagctagtcc agcatctgct
    46441 cctttttcaa cgaccatctc agctaaacgt tctggatggg tagagcctac gccgtcattg
    46501 atatttaacc cgtctggatt tgtgcccatt gtataaaaat ctgtttcaag atcggcaaac
    46561 aaacgattaa cacttgttga agttgctccg tttgctgcat cgacacaaat ggtcaaaccg
    46621 gaaaggtcac cgttgatcgt ttgtacaagg aattgagaat atttcaataa gccttctgga
    46681 aattcttcta cagttcccaa tccttcagct gatggacgag gcaattcatc cacttcagca
    46741 tctaataatg cttcaatttc tagctcttgg tcatctacta gtttaaaacc atctgcaccg
    46801 aaaaatttga ttccattatc ttgtgctgga ttatgagaag cagaaatcat gacccctgcg
    46861 cttgcttttt gcaaacgtgt cagataagca acacctggtg tagagatcac acctagctgg
    46921 aacacttcga tacccaccga taaaagtcct gaaatcagcg cttcttctaa catttgacct
    46981 gagattcttg tatcccgccc tactaacacg cgtgggcgtt ttccttcttc ttcatgctgg
    47041 cttaggacat aacctccgca ccgtcccagc ttaaatgcaa gttctggcgt aagttccttg
    47101 ttggcttcac cgcgtacgcc gtctgttcca aaatatttac ccatagttgt aaaattactc
    47161 ctttaactta attttctgag gaatttgttc tgctcatttg tgttgattcg cttgtttctg
    47221 atgtttcttc tgtacttgtg gtcgagctgc ttgtcccaga ttccgaactg gaagaaatgg
    47281 ttgttgtcga tggttccgtt cctgaatcag cagatgaaat acttctactc gtacttgtat
    47341 tttcttgtgt ccgactgctc gtcgaagcag agctactgct tgacgtttgc aacacgggtg
    47401 taatctccac actaactgat tcgggcttga tacttaaccc ctcttctact ggtatttcaa
    47461 ttttacgagt tgttcgatga ttgatacctg agacattgat cggtacagca atactgtcac
    47521 cgatcgatgc aagcaaatcg ctagagccgc ttacttctgc ttccagtttt gaaaaactga
    47581 aatcatagct ttctaccccg gcagctggtg ttccttgctg tatcccataa agtcgtaccg
    47641 acttgcttgg cttgatcgaa tcagctctca ctgttacttg cactggatca gagacgatac
    47701 ttaatacttc ccctgctgca ttcagcgcct gtattgggac ctcactatta atcccatcat
    47761 ctgtgaccat cgaagcgtcc acatcagcta ctactcgttt gatttctgcc aatgttttgt
    47821 cacctgtagt gatagagact tttttaggac taacggaagt atcatcaagt tcatatccag
    47881 atggtgtgaa cgttgaagga atcaacgttt ccacatcgaa atcttttatt acctttttct
    47941 caaccgttac tgtaatagtc gatggttcga tctttgctgt gacagcactg ctcaaatttt
    48001 gaatttttag cggaacttca tgtgttcctt cgcccagctt agtgatatct gctgtgacgc
    48061 ggaacatccg tgtatcttcg tttgcctctg cattcaactg aactcggttt gcactggaaa
    48121 gtttgacagt cactgcattt tcatatccat ggatatagta gcgctttgaa tcataagaaa
    48181 tcttgatcgg cacccccgta actgtttgat tatacgcttc atttccagaa agcgtatttg
    48241 gaacggactg gccatttgca ttgaagtaca gcgcaatact gaacagcaat gcaataaaac
    48301 tgtaaagaat gcttgtccgt ttctcttttg agatcatttg cgatcccctc ccttagtcat
    48361 accgtctagg aaagattgaa tcacgttttt cttgtttttt gttttctctt cagcaattag
    48421 ttctctacgc aagattgcca aatattcttg ctgggataag ccagccatca gttcattatc
    48481 taacgtgatg ctgacgtccc cggtttcttc agagacaacg atggtaatcg cgtcactgac
    48541 ttcgcttata ccgacagctg cccggtgtcg agtcccgaat tctttaggga tcaacatact
    48601 ttctgataga ggaagatagg cgcttgctac tgcgatcttt ccttctttta caataactgc
    48661 accgtcatgc agcggtgtat taggaataaa gatattgatc agcagctccc cagtgatatc
    48721 tgcatctaaa gcaatccctg tctcaatata ctcatctaat ccagtatgac gttcgatagt
    48781 gatcaacgca ccgatttttc gcttggacat atattggatg gctttgtcaa aagacaagat
    48841 catcttctca tcttcctgct gttcgctttt tgattgtttg aaaagcgaac tgcgtccaag
    48901 atgttccagt cctctccgga tttccggctg aaaaatcacg atagcagcta tcaccccgta
    48961 cgtaatgact tggttcatca gccaagacaa ggtgtgcaga ccaatgattt ccgctacgat
    49021 tcggataaca ataaaaatag ctacaccttt cagaagctga atcgcttttg tgcctcgcag
    49081 cagttggatc agtttgtata caagatacca gacaaccaga atatcaagta tgttcaccaa
    49141 aaaattcaga gagaacaagt caccggaaaa catttgctgc caatagtcca aattaaaaag
    49201 ttccccgatt tgaaatgaca taaaaagcct ctcttctctt ttgagatttc ctctctagta
    49261 tatcatatgt tttaatttta gaggctgata tgctcgtatt tttcttgaga aaaacgcata
    49321 attttcaaga aaattgcaag tatgcgtttt atttttctct gcaataaata tttttaaact
    49381 tcagagaatt attcattata ttttttctgt tatttttcct ttttttcttt tgtagcttgt
    49441 tccatttttt tacgtcgttc tgctctggca gctcttctag cagctcttct ttcttctgct
    49501 tcaaggtccg tcgttttttc ttttctagtc ttaacgggta catcgataga ttcctctggt
    49561 tcaagtcgtt tcttgatttt ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt
    49621 gttttagcgt cgtggacagt cttttcaaat aaagcttttg caagatttgg atttgcgtta
    49681 tctagcgagg aaaagcgaac ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt
    49741 tttttgtagt ccaaagtcat tgggttcttg tttttttcga ccattgtagg gttatatcga
    49801 tataatgacc aataaccaga agaaacagct tcttttgctt ctttaagcga ttctttcatt
    49861 ccgccaagca agccatgtgt gatacaaggc gtataggcaa taatcagaga aggaccagga
    49921 tacttttctg cttcttcaaa tgccttgatc gtttgcattt gattggcacc tgaagcaatt
    49981 tgcgccacat aaacatttcc atatgtcatc gccatcattc ctagatcttt ctttgaagca
    50041 tactttccac tagccgaaaa tttagcgatt gccgaagctg gtgttgcttt tgaggtttga
    50101 ccgccagtat tggaatagac ttcgttatcc aaaacaagga tattcacgtc tgctccgctg
    50161 gcaagtacat gatcgatccc gccaaaacca atgtcgtaag cccatccgtc tccaccaatc
    50221 atccattggc tgttttttac gaatagatcc tttctcttgt atatatcctc cagttcaggg
    50281 aaaattgatc gttcgctttc gatcgcagct tgcagttttg ctgctctttg ctgcgttcct
    50341 tcactctcaa acatgtgtac taaccagtct tccatcaatt ctcgtagttc ttctgatact
    50401 tttggcaata cttctttgat tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga
    50461 agcataccat aaccaaattc agcattatct tccaataaag aatttgacca tgcaggccct
    50521 tgaccacatt cattggtcgt ataaggagaa gcaggagaag caccgcccca aatagaagaa
    50581 cagcctgtcg cattcgcgat catcattcga tcaccgaaca tttgtgtcaa taattttaca
    50641 taaggtgttt cgccacagcc tgagcaagcg cccgagaatt caagaagcgg ttgattgaat
    50701 tgtgtagaca agacagtatt ctttcctttg actggatttg ctttttgtct taatgtcatt
    50761 gcaaaagccc aattcacagc ttgttctttc tgttcttcat aaggcttcat cactaaagct
    50821 ttttcttttg cgggacaagc ctctacacac agaccacaac ctgtacagtc ttccactgaa
    50881 acttggatac ggtattttac gccgtctgcg cctcgcattt ctctcacgat aaatccttct
    50941 ggcgcttctt ccatttcctc ttcatctgct aaaaatggac ggatggcagc atgaggacaa
    51001 acaaaggcac attcattaca catcgtacag cgatcactaa tccactcagg tacttctaaa
    51061 gcgacgcccc gcttttccat agaagccgtt cctaaaggca tttcgccagt tgtcattcca
    51121 tttgctacaa gtgtaccaac agagagttga tttccttcct gtgcattcac tggttcaagt
    51181 atttcttgta tataaccagt gttcctttta ttgatttttt caggcatctc caacgttctc
    51241 caatggtctg ggatcgcgac ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg
    51301 tttttttcta cgatagccat tgatttatgt ccatagcttt tcagggcttc ttcttttagc
    51361 agcggaagca cctcatcaaa aggaatgatg tcagctaatt taaaaaaagc cgtttccatt
    51421 gccgtattga tccggcgtcc caaaccgact tcttgggcca atctcatggc attgatcgta
    51481 tagaatctaa tgttattttc agccaagtaa cgttttaatt ttaagggcag atgcgtttct
    51541 aattgttcgt cgctccaaag agtattcaat aaaaaaatgc cacctggctt caatcctttg
    51601 accaagtcat agctatgcag gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg
    51661 acaagatacg ctgaacgtat cggcgtatct ccaaaacgaa gatgagagac agtcagaccg
    51721 ccagattttt tcgaatcata ataaaaatag ccttgcgcat atttatcggt gtgatctcca
    51781 ataatcttga tagctgattt atttgctcca actgttccgt cagatccaaa tccccaaaac
    51841 ttggcctgga atgtttgcgg ttctgtcaaa tccagacttt cctttggttc caaagattga
    51901 taagtcacat catccacgat ccctatggta aaccgttttt tgcgtatgct tgggtctttt
    51961 ttcaattcat caaaaactgc tgagatttgg tcgggagtca catctttaga ccccaatccg
    52021 tagcgaccac caataacagc cgggcgaagt tcagaatcat ataacgcact ttgaacgtct
    52081 aataagagag gttctccacc tgcccctggt tctttacttc gatcaagaac agcaattgtt
    52141 tttactgtat gaggcaactt ttgcacgaat gtttccactg gaaaaggacg ataaagatgg
    52201 atattcaaaa aacctgtttt ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc
    52261 gtctgtgcaa ctgatcccat agaaacaatg acttcttccg catcgtctgc tccgtaataa
    52321 gtcaccaagt cataagaagt tcctcgtaat cggttgatct ctttcatata tttttgaacg
    52381 atttcaggta atttatcata ataggggttg atcgtttccc gttgttgaaa atggatgtct
    52441 ggattctggt tcgtaccact aacagtcgga tgattgggat tcatccctcg tttacgaaat
    52501 gaagctaatt tctcctgatt gatcagagga gctaattctt catattccaa aacttcgatt
    52561 ttttgaatct cgtgactcgt acggaatcca tcaaagaaat tcaaaaaagg gacttctcct
    52621 tcaatagaag caagatgggc aacaggtgct aaatccatca cttcttgtac actgctttca
    52681 gctaacatag caaatcctgt ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata
    52741 ttcaacgcat ttgtcgtcac tgcccgacta gcaacgtgga aaacacttgg cagtagttcc
    52801 ccagcaatct tgtacatatt cggtatcatc aaaagcagcc cttgtgaagc ggtatatgta
    52861 gttgtcagtg caccagtttt caatgatcca tggaccacac cagctgctcc tgcttcggat
    52921 tgcatttcta ctacttttac tggttgaccg aatacgtttt cttttcctgc tgctgaccat
    52981 tgatctacta attcagccat ggtcgaactg ggtgttattg gatatattgc agcaatctcg
    53041 gtaaatgcat aagaaatata tgcggctgcc gtattgccat ccattgtttt catttttcgc
    53101 atgattcatc atccttgttc atattctctc ttattatctg ttccattata acgtaaaaca
    53161 tgccattttt ttcttttatt tataaaaata ttttacatgt atctatttga attggtcatc
    53221 taatttttag acaaacagtc taaatcgttg cctttttttg tcagacatgt ataaactata
    53281 agcgaaagag gtgttttaac atggatatat cagtgattga tgcaacgaaa acaaatacac
    53341 aaaaaggaat cctttatggt tcatcgaatg ctcctaaaaa aatggtcgaa ttcatcaact
    53401 tagcttgtcc gtattgccga caatggtttg aagaatctta tgagctatta gaagaagcag
    53461 tccaaagcgg gcaattgcag cgagtgatca aattgttcga taaagaaaaa gaaagcttac
    53521 agcgagggaa cgtgatgcac cggtatctca caatcagcga tggccaaaaa gcgatcaaag
    53581 agatcaaaca aatattcgat actcaagacg agtggaaaca tctttctctc caagaagtag
    53641 cggattttgc agtagataaa ctaaagctga cagaacaaaa agacgaacag ctgagccaag
    53701 ctgtcatcaa cgaagctgag caagcacata ttcgatttgt tccaactgtc atcttgggta
    53761 aagaaatctt tgatgaatct atttcaatag aggaattaaa agaattgatc caaacaaaat
    53821 aaaaaaataa ggagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
    53881 tataaacggc ggaagcagaa gcaactcctt cttatttctt ggagttaaac gcttctgtcc
    53941 cagcctcctt attccgaata aatggtgtcc aaaagcttgc atctgccaga ttatctcatt
    54001 tcttatgaca agctaagcaa attatctcca ccaaataatt tattcatatt tttgacttgc
    54061 gcatactaac taattcttaa attttcatac aaaatttctc aatttccctt aacgagtaat
    54121 atttatgtac ggtgattctc actagtatcc caatcacttt ttaagttatc ccgatttcaa
    54181 ttctacttca aaaatagttg cttttatgta tgctgttagt tgattagatg gtcatttgtt
    54241 gagctaggtc gtttatttta aacaagttgt ttctttagct tatacctatc tcgtctacca
    54301 aacgcaagat ggcaaacaca aatgcatacg catctgtatt tgccactttg attcgtataa
    54361 agctaacaac tattaactcc tctcagctaa tagttgctct cttttttatt cagattcatt
    54421 tttgctgatc acagcctagt atttcaatgg ttttgaacac taaaccaaat tgttccatta
    54481 caacattcac tggctgatga aatggattag tagatttcga ttattcaaag cgattttcta
    54541 ttggtggatc gtgttcccaa ctaataagac ggattccaaa cctatcgtac agatcttcta
    54601 aaccacttat aaattttgca cctatttcag gtgttttatt gcgtggatat tctattgtta
    54661 cgccattaaa tggataccaa ttaggattag gctctctttt tttctgtaca tctcccatag
    54721 aatagttagc actttctggg acataatata ttcttcgacc atcctctaat tcatctaata
    54781 agatatttcg tgatcgtcta tcacttgcta aatgtagacg ccacgtgcga taccatgcca
    54841 tgctacctgc atctgcagaa aatccatcaa ataatgcaac aattgtatct tctatcacaa
    54901 tcctttcccc atctacttca tattctaatc taaaatcaaa ctcccctcgg gtaatttctg
    54961 gtcttggtgg cttaggaccc aatattattc tacttaaaac cgcattcgtt cccagatgaa
    55021 acacagctat aacaacaata atgattaata ttttgtttct atttgataaa tttctcatta
    55081 ttcgcacatc acctctacat tctcacatat tagttcgtaa tactccttta atatcaaaat
    55141 atctactggt cgtaataata ttcgctatga attcctacta ctcgtataag aaaggccaaa
    55201 tcatcagctt ttacttgctg atgatttggc ttgtttctat ttatcgtaat tgttttctaa
    55261 tcaccaaaat tgttaacaat cggttcagat atttcccagt tgattaaggt aattccgtat
    55321 tgtgtcaaaa cttcttgcgc ttctccgaaa ttaagtacag ttcttatgta tccactttct
    55381 cctaatataa agtctacctc tcttttaaaa ccttcctcga gatttctaag ggctatatgt
    55441 ggttttaatg acattgattg tacattttcc cccatataat attgcgcact tcctcgacta
    55501 aaatagattc ctagatcgtc cccaaaaggt agatcttctc taccactagc taatctactt
    55561 ctccatcttc gttctctttt catagaacct gcgctgaact cacacccgtt aaactcagct
    55621 atgacagtat cttcaattat gttaatctgt ccatctagtt cgtattcaat cctaaatgga
    55681 aattctccat aggtgatttc cggttcgggt attttaggtg agattaatgc aaagaaagga
    55741 aatattaagc ttggcaacat caaaaaaact cctcctatga tcacaattcc cgatgtaatc
    55801 caaaatataa tttgtacaat tctcattgat ccattgtatt ccattttatt ccccctatta
    55861 ctattcatat ccgcctatct atatcgatct tccaatgcag agataattgc ttccatacct
    55921 tgatttttta ctgagttacc tatgcgagat atcattctcc actaaggtgc atcatattgt
    55981 ctatctaaca tgattaattc tccaataggt gtatactcct ccaaaaacaa catttaatgc
    56041 atctccctcg acaacataag ctaccatgtt tgctgtatat gtgtttgagt ctaattcata
    56101 agcagataaa tttactgtcg ctggattaaa tataatcgca ttcgtattta tagctactga
    56161 atttgctgct tcagcaccac cttttgaatg tccgatcatt gtgatttcaa tagtaggatt
    56221 tacgtggaca gccctcaccg tttaccctga ccattataga aatcgcttac ttttttgaac
    56281 tacgcaacaa aggatacaat gctcctaact tcactgtctc catttttgtc cactaaggaa
    56341 tataaaaaat aggcattaat tcatttaatt cggtcacaac acgttacctt ttaaatcatt
    56401 atactgtatc tcatacatta ttatcaataa actattataa aaatgcaaaa aatgctttat
    56461 caatctattt tgtaattggt cataatatca ttgaaatcat accctgtatc gatttttcct
    56521 atgaatctcg tctatttgtg attgacgaaa aagaggcaag acagacactg gaatggtaat
    56581 tttacccact attttgctcc aactatttgt aatcgtaccc acttttgaaa taccgagatg
    56641 atcattttaa ttgatatgct gattcccaag caaaacccca agaaattaaa tgctctatcg
    56701 catctagctt cttgggtttt gcttatataa attccaacat ttaaattttt tatgctgttc
    56761 ggtctcagaa atgccgtttt tgcttatatc acaatatcaa gatgtcaaat tattcgtgtt
    56821 gccgatgact ttgattaatt tttttcaaat tgtttattaa gactaatgct gtctttttgc
    56881 agataccaat gttcgaactt tttaacatta tcatatgatc ctgcaagttt taacttctta
    56941 ccattagatg ttatcaaagt tataattaac cttgcctttt tcccattagg atcttttatt
    57001 ttatgaattt tctgcaggta atctctccta atggcagcta tttttacctc atcagtgctg
    57061 ataaaccatt gatcatccac atacatactg cctgttatgt tgtgtttttc catttcttcc
    57121 gagaacgaaa agtttaaggc tcgttcttac ttccaaagtt gaatgagtcc atggattgga
    57181 cttagtgtga gaattgagaa caacaataag aatccatatc ctcccgcaaa catccctaag
    57241 gctatactgt ctctccatcc ataagtagga ttagaagaga aaagagtcac cgtttgtaat
    57301 aatatgaaaa ggaataatcc agcacaaaca ccaaagataa caactccaaa cattgaattt
    57361 ttatacaatg accatttatt tataggcttt tctttcattt tacttccccc tactgttctg
    57421 ttcacacgga atgaattcaa agttcataag ccctcctctt tgaacatatg aatacgactt
    57481 gctaactata ctttaaaaaa gcttgtttta agcggttata ggcgagttgt aatctctatt
    57541 catctcatta gcaaataact tgccgaaaag tctactgaaa ttaaagcaga tagtggaaga
    57601 attatttagc caggatagac actatttgtg aaacacatgt ctttactttt gaaattctat
    57661 gatgaaaagc cttggaatac agtagctctt aattggtaat actttttcca ttataaataa
    57721 cctcttttca ttttatattt atcccctcta aaaataatat ctaatctaat attttaaact
    57781 tatatttccc tcgctgtgct aatagagcat tactaaataa atagaaacta gaaaaaagca
    57841 ttacgcctgc cagaataaac caattatttg t
//
LOCUS       DACDMM010000003        57265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDMM010000003 DACDMM010000000
VERSION     DACDMM010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.003"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            14..382
                     /locus_tag="IWD16_000109"
     CDS             14..382
                     /locus_tag="IWD16_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP9087198.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            552..983
                     /gene="mraZ"
                     /locus_tag="IWD16_000110"
     CDS             552..983
                     /gene="mraZ"
                     /locus_tag="IWD16_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP9087199.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1004..1963
                     /gene="rsmH"
                     /locus_tag="IWD16_000111"
     CDS             1004..1963
                     /gene="rsmH"
                     /locus_tag="IWD16_000111"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP9087200.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1968..2369
                     /gene="ftsL"
                     /locus_tag="IWD16_000112"
     CDS             1968..2369
                     /gene="ftsL"
                     /locus_tag="IWD16_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9087201.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2370..4562
                     /locus_tag="IWD16_000113"
     CDS             2370..4562
                     /locus_tag="IWD16_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9087202.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4588..5550
                     /locus_tag="IWD16_000114"
     CDS             4588..5550
                     /locus_tag="IWD16_000114"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP9087203.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5564..6925
                     /locus_tag="IWD16_000115"
     CDS             5564..6925
                     /locus_tag="IWD16_000115"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP9087204.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6951..8039
                     /gene="murG"
                     /locus_tag="IWD16_000116"
     CDS             6951..8039
                     /gene="murG"
                     /locus_tag="IWD16_000116"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9087205.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8054..9274
                     /locus_tag="IWD16_000117"
     CDS             8054..9274
                     /locus_tag="IWD16_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP9087206.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9430..10755
                     /gene="ftsA"
                     /locus_tag="IWD16_000118"
     CDS             9430..10755
                     /gene="ftsA"
                     /locus_tag="IWD16_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP9087207.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10778..12019
                     /gene="ftsZ"
                     /locus_tag="IWD16_000119"
     CDS             10778..12019
                     /gene="ftsZ"
                     /locus_tag="IWD16_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP9087208.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12036..12713
                     /locus_tag="IWD16_000120"
     CDS             12036..12713
                     /locus_tag="IWD16_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP9087209.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12727..13326
                     /locus_tag="IWD16_000121"
     CDS             12727..13326
                     /locus_tag="IWD16_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP9087210.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            13341..13628
                     /locus_tag="IWD16_000122"
     CDS             13341..13628
                     /locus_tag="IWD16_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP9087211.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13677..14459
                     /locus_tag="IWD16_000123"
     CDS             13677..14459
                     /locus_tag="IWD16_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP9087212.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14480..15187
                     /locus_tag="IWD16_000124"
     CDS             14480..15187
                     /locus_tag="IWD16_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP9087213.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15449..18247
                     /gene="ileS"
                     /locus_tag="IWD16_000125"
     CDS             15449..18247
                     /gene="ileS"
                     /locus_tag="IWD16_000125"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP9087214.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(18296..19822)
                     /locus_tag="IWD16_000126"
     CDS             complement(18296..19822)
                     /locus_tag="IWD16_000126"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP9087215.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19837..20484)
                     /locus_tag="IWD16_000127"
     CDS             complement(19837..20484)
                     /locus_tag="IWD16_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP9087216.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(20668..20997)
                     /locus_tag="IWD16_000128"
     CDS             complement(20668..20997)
                     /locus_tag="IWD16_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP9087217.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            21174..21902
                     /gene="sfsA"
                     /locus_tag="IWD16_000129"
     CDS             21174..21902
                     /gene="sfsA"
                     /locus_tag="IWD16_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP9087218.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            21918..22931
                     /locus_tag="IWD16_000130"
     CDS             21918..22931
                     /locus_tag="IWD16_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9087219.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            22931..24208
                     /locus_tag="IWD16_000131"
     CDS             22931..24208
                     /locus_tag="IWD16_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9087220.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            24271..26973
                     /locus_tag="IWD16_000132"
     CDS             24271..26973
                     /locus_tag="IWD16_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9087221.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            27125..27442
                     /locus_tag="IWD16_000133"
     CDS             27125..27442
                     /locus_tag="IWD16_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9087222.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            27472..27792
                     /locus_tag="IWD16_000134"
     CDS             27472..27792
                     /locus_tag="IWD16_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9087223.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            27900..29360
                     /locus_tag="IWD16_000135"
     CDS             27900..29360
                     /locus_tag="IWD16_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9087224.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            29428..29649
                     /locus_tag="IWD16_000136"
     CDS             29428..29649
                     /locus_tag="IWD16_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087225.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            29680..29862
                     /locus_tag="IWD16_000137"
     CDS             29680..29862
                     /locus_tag="IWD16_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP9087226.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            29862..30275
                     /locus_tag="IWD16_000138"
     CDS             29862..30275
                     /locus_tag="IWD16_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP9087227.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            30398..31579
                     /locus_tag="IWD16_000139"
     CDS             30398..31579
                     /locus_tag="IWD16_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9087228.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            31968..32041
                     /locus_tag="IWD16_000140"
     tRNA            31968..32041
                     /locus_tag="IWD16_000140"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32002..32004,aa:Arg,seq:cct)
     gene            complement(32078..32278)
                     /locus_tag="IWD16_000141"
     CDS             complement(32078..32278)
                     /locus_tag="IWD16_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9087229.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(32583..33815)
                     /locus_tag="IWD16_000142"
     CDS             complement(32583..33815)
                     /locus_tag="IWD16_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9087230.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            34069..34638
                     /locus_tag="IWD16_000143"
     CDS             34069..34638
                     /locus_tag="IWD16_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9087231.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(34816..35256)
                     /locus_tag="IWD16_000144"
     CDS             complement(34816..35256)
                     /locus_tag="IWD16_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP9087232.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            35414..36178
                     /gene="map"
                     /locus_tag="IWD16_000145"
     CDS             35414..36178
                     /gene="map"
                     /locus_tag="IWD16_000145"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9087233.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            36210..37133
                     /locus_tag="IWD16_000146"
     CDS             36210..37133
                     /locus_tag="IWD16_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP9087234.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            37209..38348
                     /locus_tag="IWD16_000147"
     CDS             37209..38348
                     /locus_tag="IWD16_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP9087235.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            38341..39141
                     /locus_tag="IWD16_000148"
     CDS             38341..39141
                     /locus_tag="IWD16_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9087236.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            39141..39968
                     /locus_tag="IWD16_000149"
     CDS             39141..39968
                     /locus_tag="IWD16_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9087237.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            39946..40680
                     /locus_tag="IWD16_000150"
     CDS             39946..40680
                     /locus_tag="IWD16_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9087238.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            40780..41646
                     /gene="rfbA"
                     /locus_tag="IWD16_000151"
     CDS             40780..41646
                     /gene="rfbA"
                     /locus_tag="IWD16_000151"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP9087239.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            41660..42232
                     /gene="rfbC"
                     /locus_tag="IWD16_000152"
     CDS             41660..42232
                     /gene="rfbC"
                     /locus_tag="IWD16_000152"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP9087240.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            42254..43282
                     /gene="rfbB"
                     /locus_tag="IWD16_000153"
     CDS             42254..43282
                     /gene="rfbB"
                     /locus_tag="IWD16_000153"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP9087241.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            43380..44231
                     /gene="rfbD"
                     /locus_tag="IWD16_000154"
     CDS             43380..44231
                     /gene="rfbD"
                     /locus_tag="IWD16_000154"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP9087242.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            44266..46299
                     /locus_tag="IWD16_000155"
     CDS             44266..46299
                     /locus_tag="IWD16_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087243.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            46343..47623
                     /locus_tag="IWD16_000156"
     CDS             46343..47623
                     /locus_tag="IWD16_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9087244.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            47833..48639
                     /locus_tag="IWD16_000157"
     CDS             47833..48639
                     /locus_tag="IWD16_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9087245.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            48651..49871
                     /locus_tag="IWD16_000158"
     CDS             48651..49871
                     /locus_tag="IWD16_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9087246.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            49861..51447
                     /locus_tag="IWD16_000159"
     CDS             49861..51447
                     /locus_tag="IWD16_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9087247.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            51486..53624
                     /locus_tag="IWD16_000160"
     CDS             51486..53624
                     /locus_tag="IWD16_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9087248.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(53992..54975)
                     /locus_tag="IWD16_000161"
     CDS             complement(53992..54975)
                     /locus_tag="IWD16_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP9087249.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            55353..56744
                     /locus_tag="IWD16_000162"
     CDS             55353..56744
                     /locus_tag="IWD16_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9087250.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            56760..>57265
                     /locus_tag="IWD16_000163"
     CDS             56760..>57265
                     /locus_tag="IWD16_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9087251.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWN"
BASE COUNT        18692 a         9786 c        11980 g        16807 t
ORIGIN      
        1 ggagtttacg ttcatgggca cattcacacc acttatcttg ttttggtata tttacccggt
       61 aatcgttttg tttggttgcc aatttcttgt aaaattattt tcgttaaatc gtcgttttaa
      121 aataaaagcc cctgatttgg cgattccttt tttgtggggc ggtatgcatg cattgtctag
      181 aaatacaggg acaatttcta tcttaccgtt tttctttatt tctgttttgc taatgggaat
      241 ttgtattgct gttttccaag catattatta tgaagaaatt atttatccgc gttatttcaa
      301 gatgacttgg cgtttggttt ttctgttgac aatcgtcttg tacgctgtct tgatcattgt
      361 gaacatactt tcttacctat gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct
      421 ttttgcagct tgatttttaa aaaaatgttt atgaataaaa aaagttgttc ccggatttcc
      481 cagaaaaggt ggtagaaagt gggggattgt ggtagactaa ttttagttag tggaggaatg
      541 gggggctttc aatgttgatg ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa
      601 tcgtgccctc aaaacttcgt gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg
      661 atggctgtct tttcggctat ccgatgagcg aatgggaaaa tctggaagct aaactgaacg
      721 aaatgccgtt ggctaaaaaa gacgcacgta cttttgtgcg attcttctat tcggctgcca
      781 ctgaatgcga acttgataaa caaggacgaa tcaatatacc cagcacgtta agaaattatg
      841 cagctttgac aaaaggttgc gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg
      901 agacacgatg gcaagatttt tctgcagcag cagaagagaa ctttgacgaa attgctgaaa
      961 gtatgattga tttcggacta taagaatagg aagaggctga ctaatgactg aaacatttca
     1021 acactataca gtcatgttga aagaaacggt agatggattg aacatcaaac cggatggcgt
     1081 ttatgtagac tgtacactcg gcggtgccgg acatagtgaa tatttattaa gccaactaag
     1141 ttctaaaggg catctgtatg cgtttgacca agatcaaaaa gcaatcgatc acgccaagct
     1201 gcgtttagct tcttatgtcg aacaaggtat ggtcacattt atcaaagcga acttcagaga
     1261 attagaagaa cgcttgagtg agcatgtaga taaggtagac ggcattttgt atgatctagg
     1321 ggtttcctct cctcagctag atgaagcaga acgtggattt agttatcatc aagatgcacc
     1381 gttggatatg cgcatggatc agtcagctcc tttatccgct tatcacgtaa ttaatgaata
     1441 ttcctaccat gaattagtaa agattttctt ccgctatggc gaggagaaat tttccaaaca
     1501 aattgctcgt gaaatcgaac gagtacgaaa aacaaaacca atccaaacaa ctggtgagct
     1561 tgtcgaaatc attaaacaag tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc
     1621 aaaaagaatt tttcaagctg tgcggattgc agtaaatgac gaactcggtg ccgaagaggc
     1681 ctctttagaa caggcaatcc ggttattgaa agtcaatgga agaatcagcg taatcacatt
     1741 ccattcattg gaagacagaa ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga
     1801 tctaccaccg ggacttcctg tcatacctga cgaattccaa ccagaactga aagtcattac
     1861 tcgtaaacca atccttcctt cagaggaaga gctaacagaa aacaatcgat cacgcagtgc
     1921 aaagttacgt attgcagaaa aaataagaat gaaagaggag tagaatgatg gcagaattaa
     1981 agaaaatgca agattacgaa tatgatttac ctgaattggt ggatcagccg gaacaacaaa
     2041 tgcctgattc ttcttcgaat caagaaatca ttcttccaca atcaccggca aaacgattaa
     2101 agcagatttc tgctttggaa aaaattgctg tggcaagcgt ggcgattgct attgttgctt
     2161 tatgtgtttt aacagttatg ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt
     2221 caatacaaac agaaatcaat gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa
     2281 acgaactttc tcgtactgaa cggattaaac aaatcgcaga agaaaaagga ttgtctatta
     2341 atgacgacaa tttaaggaaa gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta
     2401 tggaaaagaa aaatctaaat ccgatgaaca atcgcaaaaa agtaggtatc attttatttg
     2461 ctacgagtat tggattgttc tttttatttg ccgtccgttt ctcgtatatc gttattggag
     2521 gtcatgttgc tggtacctca ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag
     2581 tagtaaaagc aaaaagagga acgatttatg atcgtaacgg tgtggcgctc gcagaagatg
     2641 cctcctctta ttcaattaaa gcgatactct caaaaacata tacctctggg gataaaaaac
     2701 tgtatgttga agaaaagaat tttgataaaa tagcagaaat cctgcataag aatctttcta
     2761 tagacaaaaa agatgcactg aatattttag aggacggcgc aaaaaaagag ctgtatcaag
     2821 ttgaatttgg tagctatggg aaaaatatct cccaagaaac caagcaaaac atcgaagcgg
     2881 acatgaaaaa agaaggtgtc gctggtcttt attttgtcga ccatcaggct cggatgtatc
     2941 caaatggcgt cttttcttct cattttattg gatacgcagt tccagacaaa gatgagaatg
     3001 gcttagttgg taaactaggt ttggagtctg cttacaatga tatcttgagc ggtaaagatg
     3061 gtaagattat ttatcaaaaa gataattttc aaaatcctct gccaggtaca gtggcagaag
     3121 aagaaaaagc tgtcgacgga caagatatct acacgacttt agacagcaga ctacaaagtt
     3181 atttagaaac actgatggat caagtaaatg aagaatatca gccagaagaa ttgacagcag
     3241 tcctgatgaa agcaaaaacc ggagaaatct tggcaatggg acaacgacct actttcaatc
     3301 ctgaaacaat ggaaggattg actggaaaag atgctatctg gcgtaatttc ttggtacaag
     3361 atagttatga accaggatca accatgaaag ttttcactac tgcagctgct atcgaagaag
     3421 gagaattcaa cgagaatgaa accttccaat ctggaaaaat ccaagtagaa gatgctacga
     3481 tcaatgacca tgactttggt gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt
     3541 caagtaacgt cgggatggtc atactggaac agcgattagg cggacgatgg tataactatt
     3601 tacaaaaact gggatttgga caaagtactc attctggatt agatgatgaa gtaaacggtg
     3661 ctttacctac attaaatatt gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg
     3721 tgacgaattt ccaaatgatg aaagctttca cttctattgc gaataacggt acaatgattc
     3781 aacctcgata tatcagcaaa gtggtggatc cccaaactgg agaagagcga acaactcaaa
     3841 cagaagttct agggcaacct ttttcaaaag aaacaacaga aaaagtccgt gaatatatga
     3901 gagatgttgt cgaaagcgaa aactacggta gtgcttatgg tgtatatagt gtacctggat
     3961 acaatgtatc ggcaaaaaac ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa
     4021 caggagatac agcctacctg tattcgattg tggagatggt tccttctgaa gatccagact
     4081 atgtcttgta tctaacaatg aaacacccta agacatatga tcgaatggca ttggctaaaa
     4141 tcgcaaatcc attgatgaaa cgggcaatgg attttaaaga aactgaagaa gattcagata
     4201 cagaaacaaa aacagaaaaa gtatccgtag ccgattatcg taatcttgaa gcggacgttg
     4261 cagcagctga tgctcaaaaa agtggactgc aacctgtagt gatcggtaat ggtaaaaaag
     4321 tacaaaaaca atctactgcc aacggtgatc agttgatctc tggagaaaaa ctgatcctat
     4381 atactggtgg tgataagctg atgcctgacg taacaggatg gtcaaaagca gatatcatga
     4441 agctggggaa aatcctcggt attgaagtta gttttgatgg agatggttat tgtgtcaaac
     4501 aagaacttgc tccatatgaa aaaatcactg aagacaaact tagttttact ttagaagaat
     4561 aagaagagaa ataggagaga aaagagcatg gaatggacac aagcattgat tcccattgtc
     4621 agcagttgtg caatgacaat tgccgcaatg ccgctattta ttggttattt tcaaatgaaa
     4681 aagcaaggtc aggcgattcg tgaggaggga ccaaagtggc acaatagcaa agccggaact
     4741 cctacaatgg gcggcttagt gtttctgatc ggctctattt tgacaggaat ttgggttgga
     4801 gcttggcaaa aacagctaac tcctactctt ttcatcttgc tttttgtatt agcgttatac
     4861 ggagtaatcg gctttcttga tgattttata aaaatattca agaaaagaaa catgggactg
     4921 aactcgaagc agaaattgat tggacagatt ctagggggaa tcatctttta tcttgtttac
     4981 cgttctgaag gttatccagg tgtattgaat ttcttcggac tagatctacc tttaggaatc
     5041 atttatggta ttttcgctct cttctggctt gttggatttt caaatgcagt aaatctgaca
     5101 gatggcatcg atggattagt tgctggctta ggaagtatct cttttgcagc ttacggtcta
     5161 attgcttggc atcagcatca gtatgatgta ttaatcattt gcttaagtgt tttaggaggt
     5221 ttactgggtt tcttcgtcta caacagaaaa cctgccaaga tttttatggg agatgtcggt
     5281 tcattagcgt tgggcggact tttagcggca atttctataa tgctaaacca agaatggaca
     5341 ttgcttttga ttggtttgat ctatgttatg gaaacagcaa gtgtgatgct tcaagttact
     5401 tcctttaagc ttacaggaaa acgaattttt aaaatgtctc caattcatca tcactttgaa
     5461 atgtgcggat ggtcggagtg gaagattgat acagtttttt ggttgatcag cattgtgact
     5521 tcgttgatca cattatggat cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac
     5581 atatgaaaat aagaaagtgc ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa
     5641 attattacat gaattgggcg cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa
     5701 tccggaagca caagagctgc tttctttagg gataaaagta ataacaggta gtcatccaat
     5761 cgaattgttg gatgaagaat tttcattgat ggtcaaaaat ccagggattc cctacagtca
     5821 tcctttagta gcaaaagccc aagaaatggg tattccggta attacggaag ttgagctagc
     5881 ttacgaagta gcggaatgcc caattattgg gattacagga acgaacggaa aaacaacgac
     5941 aacaacgatg acaggactac tactgaatgc aggagcagat caaggaattg cacgtttagc
     6001 gggtaatatt gggtatccag caagtggtgt agcgcaagaa gcaaaatcag aagataaaat
     6061 tgtgatggaa ttgtctagct tccaactcat gggaatcaca gatttccgcc ctcatatagc
     6121 ggtgattacc aatatctatg aagcacatat tgattatcat ggtacaagaa aagaatatgt
     6181 caaagctaaa tggaaccttc aaaaaaacat gacagaaaaa gattatctga tccttaactg
     6241 gaatcagtca gaactacaag aattagcgca aagaacaaaa gcacgcgtac tgcctttctc
     6301 gacaaaagaa gtgttggaag acggtgtgta tgctgacgat tactcaatat actataaaaa
     6361 agaaaaaata atggaaatat ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc
     6421 actagctgct atttctgtag caaaattgta tggtatatca aatgaagcta tacgagaaac
     6481 gttgcatttt ttccatggcg taccacatcg tacgcaatat gtaggagaaa tccaaggcag
     6541 aaaattttat aatgattcaa aagcaaccaa tattttagcg actaaaatgg ctctaagtgg
     6601 atttgaaacg tctaaggtcg tgttattggc tggcggatta gacagaggaa atacatttga
     6661 tgaattaatt ccttcattaa aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga
     6721 aaagttgatg gatgctggaa aaaaagcagg catcgagaca atagtaacag ccgattctgt
     6781 agaacaagca gttcctttag cattagaaaa cagtacagat ggtgatgtag ttttattatc
     6841 accagcgaat gccagctggg atcaataccc aaattttgaa actagaggaa acagattcat
     6901 ggaagctgtg aaccgattaa aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt
     6961 tggttacagg cggaggaaca gggggacata tttatccagc tcttgccttt gtcaattatg
     7021 taaagactca agaaccgaat gctgaattca tgtatgtggg agcaaaacga ggtctagaaa
     7081 acaaaatcgt ccctgataca ggaatgcctt tccatacctt ggagattcaa ggatttaaac
     7141 gaaaaatttc catgcataac atcaagacgg tccgattgtt tcttaagagt atccgtcaag
     7201 caaaaaaaat cctcagtgat tttcaaccag atatcgttat cggtactgga ggatatgttt
     7261 ccggagcggt agtgtacgct gcagccaaaa tgggtattcc aacagttatc catgaacaaa
     7321 acagtgtacc aggtgttacc aataaatttt taagccgata tgtggatcgg atcgctcttt
     7381 catttaaaga tgcggcttcc ttttttcctg aaaacaaagc agttcttgta ggtaatcctc
     7441 gagcacaaga agtagcagat actaaaaaat cagatatttt gaaaacattt gatttggatc
     7501 cagaaaagaa aacagtcttg atttttggcg gtagtcaagg agcacttaaa atcaatcaag
     7561 cagtatcagt ttttttaagt gaatatattc cttcttctta ccaagttttg tatgcttcag
     7621 gagatagata ttatgaagag atcctaaata aaataaatcc tgaaatacaa aatattagtg
     7681 tccagcctta tatcaaaaat atggcagagg tcatggcttc cagtgatctg ctggtggggc
     7741 gtgctggtgc aacttcgatc gcagaattca cagctctggg gttaccggca gtgttggttc
     7801 ccagcccata tgttacgaat gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag
     7861 gagcagcaaa aatgattgca gataatgaac taacaggtga atcactaagt cagacagtca
     7921 acgaaatcat gggtgatgaa gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag
     7981 gaatccctga tgcttcaaaa cgtctttatg acttagtaaa acaaataatc aaaaaataaa
     8041 ggcggtggtc ttagtgagta agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga
     8101 tgaaaacaat ttgactcctt ggcaaaaggc taaccgaaaa tatctggcag aacaagagat
     8161 gaaaaaccaa gagccaaaaa aagaagacat aacattagat acaccccttt cagattctga
     8221 agaagaaaaa acggttcaag aggaacagca agaagaaagc cagtccgaga aaaaaatcga
     8281 attttttgaa gagatcaagc aaaagaaaaa cggtggacca tataacggtt cttttttgaa
     8341 ccggctgccc aatttaaaaa gtcaacggca caaagtacta tttcgtcggt tagctttgat
     8401 catcactgta ttgggtatac cgctgatttt tttgatctat tatgtttctc cttatagcaa
     8461 attacaagca gttgcagtat caggcaataa gacagtcaac agccaagaaa tcatctctga
     8521 tacaaaattg tcattaggag aaaatgtttg gggacagtat tttcatcgat ctacttatat
     8581 cgatcgcctt aaaaaagcac agccaagaat cgaaacagca aatatacatt tcaaagggat
     8641 gaatgaattc gatttggatg taacagaata taaagagatt gctttgatcg caaaaaacaa
     8701 tcaatactac ccagtgattg aaaatggaac agtattagat gaaaaagtag caaatcctac
     8761 aaaaaatctc cctatcttgg aagagttcaa agactctgca aaaatcaaag aattaacaaa
     8821 gcaatacaat cagttatcat cagaattaca aaaagcaatt tctgaaatca agtacacgcc
     8881 aagagcaagc aataaaaatc tgatccaatt gaatatgaat gacggcaatc aagttattgt
     8941 aaatatcaat aatcttgcga accaaatgaa atattattcg caagtagcca aagatatgga
     9001 tgaaaaaggt gtgatcgaca tggaagttgg tatcttctcg catccttacg gtagcactca
     9061 agaaacggaa gaatcgggcc aatcggaaga aacaactgaa agtgaagaaa atgcggatca
     9121 aacaaacgaa tcaagtgaag aggcagaaac gcaaaaagca caaacacctg gatcgtctag
     9181 cacaaacgga tcatctgatt caaatgattc aagtgaaaca agtcaaatag aagataatcc
     9241 aagtacaact ccgtcaagtt taaatgaaaa ttgataaaac aactttaaaa agtaagaatg
     9301 tactttctaa aaaccagtaa tttatggtag aattggaagc agtgaatata tctattaata
     9361 agtatatttt aaaacggacg ctagttgttc gcttgtatat ggaatagtaa gtaggaggga
     9421 atcccattca tggcaaaaac aggaatgtat gtaggccttg atattggaac gacatctgtt
     9481 aaagttgtcg tggctgaata tatcgacagc caaatgaata ttattggagt aggaaacgca
     9541 aaatcagaag ggattaaccg aggcattatc gttgatatcg ataaaacggt ccaagcgata
     9601 caacgagcag tacgacaagc agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc
     9661 ggtttgccag caaacatgct tgaagtagaa aattgccagg gaatgattgc agttaacgga
     9721 gattcaaagg aaatcaccga tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt
     9781 tcaatacctc ctgaacgtca aatcgtgtca atattgccac aagattttac tgtcgatggg
     9841 ttcgagggaa tcaaagatcc tcgtggaatg atcggcgtaa gattagaaat gtacggtctg
     9901 ttgtttacag gacctaaaac gatcatccat aacatacgta aatgcgtgga aaatgccgga
     9961 ttaatcgtca acgaaatggt gattactcct ttagcgctga ctgaatcaat tctttctgac
    10021 ggagaaaaag attttggtac gatcgtcatt gatatgggtg gcggccaaac aacaacggct
    10081 gttatgcatg ataagcaatt gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc
    10141 actaaagata tctctattgt attaaatacg tcatttaaca atgcagaagc gttgaaaata
    10201 aactacgggg atgcataccc agaacggaca tcaccagatg aagaatttcc agtagatgtg
    10261 attggtcagt cagaaccagt gaaagtcgat gaacgctact tgtcagaaat tatttcagct
    10321 cgaatggaac agattttcaa caaagcaaaa gatgcattgg accaaatcga agcattagag
    10381 ttgccaggtg gcgtcgtatt gacaggcgga gctgctagtc ttcctggtgt tgtggacttg
    10441 gcacaagaaa tcttcggcgt aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga
    10501 aacccggtat ttacaaatgt aataagtatc gtggattatt cagcaaactt gagtgaagtg
    10561 tatcaattgg caaaaattgc tgttacgggt gaaacctcag cagctcgtca aatggtagtt
    10621 gaacaagaaa caacaaacac atatgagagc tatgaagcac ctgaagaaac aatctacgat
    10681 gagccggaac caaaagagtc cggagaaaat gtaaaaaata aaatcaaggg tttctttaca
    10741 aatatttttg attaattcat cctgggagga ataagatatg gaattctcaa ttgataataa
    10801 tatcaatgac ggcgcagtaa tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt
    10861 aaaccgaatg attgaagaaa acgttaaagg tgttgaattc attacagcca acacagacgt
    10921 gcaagcatta aaaaattcaa aagctgaaac agttatccaa ttaggcccca aatatactcg
    10981 tggtttaggt gctggttctc agccagaagt tggacaaaaa gcggcagaag aaagtgaaca
    11041 atccttacgc gaagctttag acggtgcaga tatgattttc atcactgcag gaatgggtgg
    11101 aggaaccggt acaggtgctg caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt
    11161 gactgttggt gtcgtgacac gtccattcac ttttgaagga ccgaaacgtg gccgttttgc
    11221 tgcagaaggt atcgctcgtt tgaaagaaaa tgtggataca ttattaatta tctcaaataa
    11281 ccgtttatta gaagttgtag ataaaaagac accgatgctt gaagctttcc gtgaagcaga
    11341 taatgtttta cgacaaggtg tacaaggtat ctctgatttg atcacagcgc caggttacgt
    11401 taaccttgac tttgctgacg tgaaaacagt gatggaaaac caaggtacag cattgatggg
    11461 gatcggtgtg gctagtggag aagatcgcgt agtagaagca acgaaaaaag caatctcctc
    11521 tccattgctt gaaacatcta ttgatggagc tgaacaagta cttcttaaca tcacaggcgg
    11581 tttggatatg acattgttcg aagcacaaga tgcttcagat atcgtagcaa atgcagcaac
    11641 tggcgatgta aatatcattc taggtacatc tatcaatgaa gaaatgggcg atgaaatccg
    11701 tgtgacggtc atcgcaacag gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc
    11761 agctagacaa gcacaaatgc aatcacctgc tcaaaagact gtattagata tggatcaagc
    11821 taaaccaact tcttcagaag aagaaaacag ttttggcgat tgggatatca gacgtgaaca
    11881 aaatgttcgt cctagagttg atgattccaa ttttgacaat atcgaaaaaa aagaatttga
    11941 tacattcaac cgcgaagaaa caaaatctca tggcgatgat gaattaaata cgccaccgtt
    12001 tttccgtcgt aaaaaataaa ggagaggaag agcacatgat tgctgacaac ttgcagaaaa
    12061 tcaagcaaga aattcaggat tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat
    12121 tagtagctgt aacaaaatct gttgaatccg ctcaagctga agaattggtt agagctggcg
    12181 ttatgaattg tgcagaaaac cgagtagata aattattaga aaagaaacaa gatttatttg
    12241 attttcctga aataaagtgg catttaattg gtaatttaca gcgcagaaag gtaaaattga
    12301 ttgtaaatga aattgattac tttcatgcgt tggacagttt aagattagca gaagaaatcc
    12361 agaagcggtt agagaaagaa ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat
    12421 caaaacatgg aatttcacca gatgagcttt taccgtttgt ggaatcttta gctgcctgca
    12481 caaaaatcaa ggttgttggg ttgatgacta tggcgcctaa agaagcttct gatgagcaaa
    12541 tccataaagt gtttggtacg ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg
    12601 cccatgcacc atgtagtgaa ttaagtatgg ggatgagtca agactttcaa attgcgattg
    12661 aagaaggtgc aacatttatc cgtgtaggaa cagcgctatt caaagacgaa taaaggagga
    12721 acaaaaatgc cgcttttaaa cagaggagca attgctaatt ttttcggatt agctgatgag
    12781 gaagagtacg aataccaaga ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa
    12841 aaaaatacaa cacagaatca atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa
    12901 caaaaaacgg cgacttcacg tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa
    12961 aaagtaattg aactacatca atcacctaca aaaactgtag cgaaagaaag aacagcttca
    13021 agaacctcta cacaacctaa agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga
    13081 gcttattctg aagctatggc gattgctaaa cgaatcattg ctggagaagc aaccttagtg
    13141 aattttcatt tagtagaaga aaaacaagcg agaagaatcg ttgattttct aacaggaaca
    13201 gtttatgcac tagatggaga tatcaaacgt gttggagatg agatttttct ttgtacaccg
    13261 actgggatgg agattgacag ttcaaccgct caatcttttg cagatgcgaa tttgtttgat
    13321 ctgtaagagg aggaactacg atttatacta ttgcactatt acttcattta cttcaacggg
    13381 cagcctattt ctatagcatt atcttgatgg tatatgcatt gctctcttgg tttccaggcg
    13441 ggtatcaatc agggctagga cgattattag ctaagatctg cgaaccgtat atcagtttgt
    13501 tcgatcgtct gcctttgagg attggaccct tggatttcac aattttaggg gcaatcctga
    13561 tcttgaactt agccgtacaa ggtttagcac agatcgttat atggataact gtccatttag
    13621 tttattgatt gaagcaacaa aatctttatt ctcagaatag ataggagggg aaaattttgg
    13681 atgcaaatgt ataccagcac tttcgggcag atgaacgtac atttatcgat tcggtaggcg
    13741 attggattga acaggttcaa ggacaatatg caccctatct gaccgaattt cttgacccaa
    13801 gacaatccta tattttagaa acactgattc gacaaagcag tgatttgcgt ttcatgtttt
    13861 atggcggata tgaacaggca gaaagaaaaa gatgtttgat tttccccgat tattacgaac
    13921 ctgtagagga agattttgaa acagcattgt atgaaatcca ttatcctagc aaatttgcca
    13981 gcctaagtca tgggaaaatc cttggaactt tagtgggaac ggggatcaaa cgctcttact
    14041 ttggcgatat catctcggac ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc
    14101 attttgttgc tgcacaagta acaaagattg gaaaggttgc tgtacgcttg gaaccaatca
    14161 aatacacaga aatgattatg ccgaaagata gctggacaca agaaagagga acagttagtt
    14221 ccatgcgact agacagtgtg atctctgaga tttataatat ttccagacag cggtccaagc
    14281 agctgatcga agctggtaaa gtaaaagtga actggacaga aaatacaaga cctgattttg
    14341 tacttgaact attagatatt atttcagtca gaggttatgg cagagtccag atccaagaac
    14401 ttgaggggaa gacgaaaaaa gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa
    14461 ataaaaaggt ggtttactca tggctttaac tccattagat atccaaaata agacattccc
    14521 gacaaaaatg cggggatata atcaagacga agtagatgat ttcttagatt tagttgttcg
    14581 tgactatgag gaattgactc aaagaaatcg agaattagaa aaagcagtca aacattctga
    14641 agaaaaactt gagtacttca atgaattaaa agatgctttg aaccaatcaa tcatcgtagc
    14701 tcaagatacg gctgataaag taaaaacaag tgcaagcaag gaatccgaag tgatcgttac
    14761 ttctgcacaa aacaaagcag atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt
    14821 aacaactgat gcagaagaaa aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag
    14881 acagcttgcg acagaaactg aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat
    14941 cagcttgatg cttgagtctc aattagaaca agtgaaaagt ccggaatggg atgaaatttt
    15001 acaaccattc tccagctacg tgactgacag ccatgaagtg atcaaagaag ttttagcaaa
    15061 acagcttgac aatgaaaatg atactgatgt aaactcagac acaggagtca tagaagcacc
    15121 tatgacggca tttgatacac aggctattga tatctcgatc gtgcctggtg aaaatgaaaa
    15181 cgagtagaac agaaaagcac tttgttattc ttaaagcgag ccgatgagag tggaagttcg
    15241 gtaagacact gcatggtgaa agatcctcta cttgagacct gcttttccga aattagagta
    15301 agaagcagcg gctgacctcg ttattggtca cagagagttt gcactagata gatgcaacta
    15361 aatttgggtg gtaccacggc atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg
    15421 gaaaacaaat aaaggagtgt atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa
    15481 aacaaaattc cctatgcgtg gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg
    15541 ggaagaaaat cgaatctacc aaaaaagaca agagttgaac gaaggaaaac ctagctttgt
    15601 tcttcatgat ggacctccat atgccaatgg taatatccat ttaggacatt cattgaataa
    15661 aatcagtaaa gacattatca ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt
    15721 tccaggatgg gatacacatg gattgccgat tgaacaagta ttggctaata aaggcgtgaa
    15781 acgtaaagaa atgtccatgg cagaatactt ggaaaaatgt cgtgaatatg cactttccca
    15841 agtagataag caacgagcag atttcaaacg cctaggagtt gcaggagatt gggaacatcc
    15901 ttatgttaca ttagatcctt cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc
    15961 tgaaaaaggc tatatttaca aaggattgaa accaatttac tggtctccgt caagtgagtc
    16021 ttctttagcg gaagctgaaa ttgaatataa agatgtaaaa tctccatcta tttttgtagc
    16081 tttcaatgtc aaagacggca aagatattct tgatgaagat acagcttttg tcatttggac
    16141 gacaacacca tggacattgc cagcaaacca aggtattgct gtaaatccaa aatttacgta
    16201 tgtattggtg gaagccgatg gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt
    16261 ccaatcagaa atcggttggg aaaatgtaac agttctaaaa gaatttgctg gacaagaaat
    16321 ggaatatatg actgcaagtc atccattcta tgaacgtgaa tcacttgtga ttttaggcga
    16381 tcatgttact ttagacgctg gtactggatt agtccatact gctccaggac atggggaaga
    16441 tgactatatc gctggtagca aatataaact atctgtagtg tcacctgtag actcaaaagg
    16501 attgtttaca gaagaagcac ctggatttga aggtatcttt tatgacaaag caaatcctat
    16561 gatcactgat ctattaaaag aaaaaggagc tttgctgaag ctagatttct ttactcatag
    16621 ttatcctcac gattggcgga cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt
    16681 tgcatctatc gataaattcc gtcagaatat tttggatgaa gtagaaaaag tagagtgggt
    16741 tatcccatgg ggtaaaacac gtttgtataa tatgatccgt gaccgcggcg attgggtaat
    16801 ttcccgtcag cgtgcatggg gagtaccact tccgattttc tatgctgaaa atggagaagc
    16861 aatcatcaca ccagaaacaa tcgaacatgt ggcaaaatta tttgctgaac atggatcaat
    16921 tatctggttt gaaaaagaag cgaaagaact tcttcctgaa ggatttaccc acccaggttc
    16981 tcctaatggc gaatttacaa aagaaaaaga tatcatggat gtctggtttg attctggttc
    17041 ttctcatgaa gctgttttac gtcagcgacc agaattatca ttcccagcag atatgtattt
    17101 agaaggatca gaccaatatc gtggctggtt caactcaagt atcacgacta gtgtggctat
    17161 caatggtgta gcaccataca aatcagttat ttctcaagga tttgctttag atggtgaagg
    17221 ccgtaagatg agtaaatctt taggaaacat cattgcacca gaaaaagtga tcaaacaatt
    17281 tggtgcagat atccttcgct tatgggtatc tagtgtagat tatgaagcag atgttcgtgt
    17341 ttctatggat atcttaagtc aagtggcaga agtatatcgt aaaatcagaa atacgatgcg
    17401 tttcttatta gcgaatacag aagatttcga tcctaaaaaa gatactgttt cgtacaatga
    17461 tttacgttct gtagacaaat atatgaccgt tcgattgaac caagtaatca aagaaatccg
    17521 agaagaaggg tacgacaaat ataacttcat gcatatttac cgtacagtta tgaacttctt
    17581 gacggttgat ctatcgtcat tctacctgga ttttgctaaa gatgtagttt atattgaagc
    17641 agaagatgat tatcaacgtc gttgcatgca aactgttttc tatcaaacag ctgtagcttt
    17701 gacaaaatta ttgactccta tcattccgca tacagcagaa gaaatctggt cattcctgaa
    17761 agaagaagag gagtacgttc agttaagcga gtttcctggt tatgaagagt ttgcaaacca
    17821 agaagaattg atggatacct ggactgcttt catggacttc cgtgataatg tcttaaaagc
    17881 attggaagaa gcaagaaaca gcaaattgat tggtaaatct ttggaagcaa aactaactgt
    17941 ttatccaaaa caacaagttc gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt
    18001 attgatcgtt tcaccagact actttgaagt tatggcagca gatgaacaag tgccagataa
    18061 cgctatggta tttgatgatg ttgctattac agtggaaaaa gcagacgggg aaacttgcga
    18121 tcgctgtcgc caagttcgta aagatgtagg agtagatgaa aaacttcctc atctatgcgg
    18181 tcgttgtgca aaaatcgttg aagacttcta tcctgaagct gtagctgaag gattcgaaga
    18241 aaagtaaaag ggaaaagggg gtgtgacatg ttctttgtca cacccccttt tgttatcaat
    18301 cgtttaattt tccacgttca cgataccagt tatctggttg ccagatccat tcgaatccgt
    18361 ctttttcaag tagttcgaaa gctgctttag gtcccattga tccagctttg tagttgggaa
    18421 aatccggttg tgtttggtcc caagcttttc gaatgacatc cacgatatgc caagattgag
    18481 cgacttcttc ccaatgagag aagttcgttc catcaccatt caacgcatct aatagtaatt
    18541 tttcgtaagc ttctgggcta ttttcaacca tttctgcact atttctaaat tccaatttga
    18601 ttggttccgt ttcaaatcct tgccctactt ctttcccatt caaggataaa gaaaatcctt
    18661 cagttggctg aatatagatc gttagaacat taggtggaag tgtcgtatcg tcacatggtt
    18721 catcgacaga tgttttgaat acattaacag gaacttgttt gaatacgatg ttgatccgag
    18781 tccctttttc agtcaaacgt ttgcctgtac gtacatagaa aggaacacct gaccaacgga
    18841 agttatcgat agtgaatttg cctgcgacga atgtttcagt ctctgattga gcatccactt
    18901 tgtcttcttc gcgatagccc ttgaattgta caccatccaa ttcaccttga gcatattgcc
    18961 cgcgaacgaa gttttctaac acttcttctt cgttataaat gcggatagct ttcaatgctt
    19021 tgatcttttc tgtacggatt tctttttcgg aaaatgctac tggcggttcc attgcaagta
    19081 atgctacaac ctgtaaaata tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt
    19141 agtagccccc gcgatcttct acaccgaggt tttcgccaaa agtaatttgg atattgtcga
    19201 tatagcgatt gttccattga gcctcaaaaa tattattggc aaaacggata gctgagatgt
    19261 tttggatcat ttcttttcct aaataatgat caatcctgaa aatatcttgt tctgggaata
    19321 cttctctgat ttgctggttc aattcaaaag cagactggta atcagaaccg aacggttttt
    19381 ctatgatcaa acgattgaag ccattttttg tcataatatt ttgtgatttc aaatgagaaa
    19441 cgatcgttcc aaaaaattga ggtgccatag ccaaataata gacacggtta ccgtttaatt
    19501 gatagtgtcc atctaatcgg tcagacaatt cttttaatgt ctgataatgc gttgaatcat
    19561 ttacatcatg ggattgatag taaaaatggc ttgcaaaggt agttgcttct tcatctgaag
    19621 gattcaatgc acggattgtt tctttgacaa cttcacggta gtgctcatcg ctccagggac
    19681 ggcgagcggt tccgattaca gcaaaatgct cgcctaaatt tccttttctg taaaggcgga
    19741 acaaagacgg gtacaattta cgctgggcta ggtcacctgt accgccgaat atcgtaaaca
    19801 atacattttt ttcattagac atgtggatac actcctttat attttatcga caaaaatcgt
    19861 gctggccgcc ttaaagctga cggggatttt ctttctatca ttttggatag tgatcggtcc
    19921 ttcataagct gcgatgtcaa cgatctcata ttcttcgttg atattcaggt caatagtgac
    19981 taagtaatcc aacaattctt tttcatcaag cactcgtgcg atacgcactt ttgtaccgac
    20041 aggataggac tccaatgttt gtcggcgttc ttcatggaca acttcctctt ttttaggaat
    20101 cgatcctcca tgaggacaat gttcaggatg attcaaatag ttttctaaat gatctgcaag
    20161 atgttccgaa gtcacatgtt ccaatacttc agcatcatca tggacttcat tccagctata
    20221 gtctaaatgt tcaactaaaa aaacttccca taagcgatgt ttgcgaatca gactgctagc
    20281 tttttgcagt ccgcttgcag tcaattgtac accttgataa ggggaatgtt ctacaagctc
    20341 ttctttcaca agtttagaaa tcatttcact gacagatgca gctgagacat ctagactaga
    20401 tacgatttgt ttattgttga ctttcgttgt atctccacct agttctaaaa tgattttcaa
    20461 ataatcttct cgatttggtg tcatattcga tcatcctttc atggtaaaaa accatcatta
    20521 gtaagtgtaa caaaaaaaac gttaattgac tatttttcta aataaaaaac aaaggatttt
    20581 tagacgtttt tacaacaaaa aacgaaagat gatagagaag actcattctt tcgtttataa
    20641 aggatatata tatacattgg gagggatcta tttttcagat aacaacttat atgaagtgtc
    20701 tgcagattcg tcataaagag tttgttcggg ataagaaaag ataagataaa aaccatactg
    20761 atcacgtacg actaaacgat attcatctat ttctaccaca gatgtgccaa gagactgacc
    20821 gttgatcaaa aacgtgtaat cagatgtaat ctgtaattgt tgtttttgct ggctatcact
    20881 aaagaaccat gtaccttgat actttgagag tccttctgac ttttccgctt gttttttctt
    20941 tttcaagtaa ataaaacttg agagaacggt aatgagtgaa ccaagaaaat agatcatttt
    21001 ttttatcacc aaatcactcc tttatgagaa aataatcgca tagacatatt ctttcttctt
    21061 tatttcattg taccgaataa aagctaaaat ttctctaaga ctttcgactg gtttttttac
    21121 tgtttttcta caaattatga taggatacat agtaggaaga aaggtgacat aatttgaagt
    21181 atcagcaagt ggaaatagct ttttttattg accgcccgaa tcggtttata gcacattgta
    21241 tgaaaaaaga cggagaaata attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg
    21301 tcctgatacc aggcgctgaa gtggctattg tatttgctcc tggacctaaa cggaaaaccg
    21361 cctatgatct gattgctgta aaaaagaagg gtgactggtt taatatcgat agtcaattgc
    21421 ctaatcggct ggcaattgat ggtattttag acggcacgat ccttttacca ggattaactg
    21481 gtccatttac ggtatacaag cgtgaagtaa cttatcttca ctccaaattt gatatttatt
    21541 tagagacggt ggtcggacaa aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata
    21601 aagcgattgg tgcttttcct gatgcgccca cactcagagg attgaaacat ataggagaat
    21661 taacgtatgc cgcacaagat ggttatgccg cttatgtctt gtttattgct cagttcgaac
    21721 atcttcatct ggcaacgatc catgaagaaa tgcaaccagc actagctgat atggttcgac
    21781 atgcgcagca aagcggagtt caaatcctcg cctataattg tcaagtatcg gcagatgagg
    21841 taactgtcaa gcaggccatt ccatttgatg tagatatccc ttttgaagat cctatacaat
    21901 agaaagaagg taaaataatg atcaacttag gtattatcgg aacaaattgg attactcatc
    21961 aatttgtcca agcagcttta agcacaaaaa aatatgaatt gacagcggta tattctagac
    22021 atttggaaaa agcacaggaa tttggttctc attatgaagg ggatatcgaa ttcgcaacag
    22081 atttgaagac ttttttcaat attactcata tggacaccat ttatattgct tctccgaata
    22141 gtcttcactt tagtcaagct aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa
    22201 agccagcttt ttcgacgcca aaagaaatgg atgaaatcat ccatttggca aatgaacaca
    22261 atgtgtattt ttttgaagca gcaagaaata tccatgaatc aggttttaaa aagattgccg
    22321 attacttacc attaaaagac gagataatcg gtgcaaattt cagctatatg aaatattctt
    22381 cgcgctatga ccaagtatta gatggagaag aaccgaatat cttctcgact catttctcag
    22441 gtggtgcatt gatggattta ggtgtttacc ttgtatatgc agctgttgct tggtttggaa
    22501 tgccaaaaga agtccattat ttcccaagaa agatttctac tggtgtagat ggtcttggat
    22561 gggggatctt acgttatgag aattttgatg tatcgatcca gccaggtaaa atcggtgatt
    22621 cttatttacc gtcagagatt tattttgact caggaacgct agtgatgaat ggagtcaatg
    22681 caattgaaca agcacaattt catgatcgcg cgcataaaga aatcattgat ttggcggtaa
    22741 cagctgcaga aaatccaatg gcagatgaag cagaggattt tgccaatgtc atggctcatc
    22801 cgaaagatcc agcttgggga ttgttgtatg aagagtggat cgagctagct agaaatgtga
    22861 accaagtgat ttatgattta agaaaaaatg gcggcatcaa attcgatgca gataatgagg
    22921 aagattttta atgaacgaat ttgtacaagt atttcccgaa aattggcaga aaaaatatga
    22981 ggataaaggg tttatctcac cttctcttat tcaacgagca gtttttcagc cactaagtaa
    23041 taaacaaagt atagtagcgg tttctcctac aggttcaggt aaaacactgg cttatctttg
    23101 gcctctacta ctaaacgtgg aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc
    23161 tcaagaacta gctattcaag tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt
    23221 gaaagtccaa tctttggtgg gcggagccaa cgtcaaacgt cagattgagg gtttgaagga
    23281 aaaaccagaa atcttgattg gtacaccagg aagaatattg gaattgatga aagcaaaaaa
    23341 aataaaagca catcaagtga aaacaatggt atttgatgaa gcagaccagc tatttgacgc
    23401 tgggaacagc caaatcattg atcaaattct tcatcaagct ccaacagagt atcagctagc
    23461 ttttttttca gcgacagcag atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac
    23521 gtttgagaca attgacgtga cagcagagga cgattctcga aaaggactga gacattactt
    23581 tttacgtgta ccgatacgaa agaaagatga ctacttgaga cgtctcacgc atatagaaga
    23641 ttttcatggt ctgattttct ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt
    23701 gctgtataga ggtgtaccgg taggaagtct ggcctctgat cagaacaagc tactccgaaa
    23761 agcagcgcta gaacaattca aaaagaaaat ggtcagagca cttttgacca cagatgtggc
    23821 ggctagaggt ttagatatta caggtcttcc ttatgtagtg aatgcagaag ttccgttaag
    23881 tgaagaagct tatttgcatc gttcgggaag aactggtcga atgggtaatg aaggaatagt
    23941 aattacactc gtacaagata ataatctaag agatttaaaa cggcttttac gtccatcaaa
    24001 tataacactt gaagagattt ttttatacgg aggtaagctg caaactgagc agccaaccaa
    24061 agaagatgca gctcctgcag taaataaaaa gtatgcgaaa actttttctg gaaaggagca
    24121 gaacccagaa tataacaaag aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga
    24181 gagaaataaa ggagcaaggc gcaaataagc gcctttttat gctatgatta tgaaaacggg
    24241 aacaaaattg gttgccagag gaggggttat gtgaaagaac gtcaagaaga gatcttgcag
    24301 ctattgacag aaaaccaaca aggattgaca gcaagtgatg tagctgagcg cttaactatc
    24361 gatcgaagca atgcaagccg atatttaagt gaactataca aggcccatca tattgtaaaa
    24421 accgctggcc gaccagtcgt ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg
    24481 catgtcgact cttctacgca agtgactttt gaaacactcg ttggagaaaa tgattcttta
    24541 aaagtcagca tacagcaggc aaaagctgcg attctttatc caccgcgggg gttgcatacg
    24601 attatttttg gagaaactgg tacagggaag tcgatgtttg ctgaatgtat gtatcatttt
    24661 gcaatcgatt cagagatgct ttctgcagat gcgccatttg tttctttcaa ttgtgcggat
    24721 tatgctcaaa atccgcaact gctctttgga cacatctttg ggatcaaaaa aggggcttac
    24781 acaggagcag ctcaagatag tcccggtcta atagccaaag cagacggcgg tatattgttt
    24841 ttagatgaga tccaccgttt accgccggaa gggcaggaaa tgctgtttac tttcattgat
    24901 aaaggtattt accgtccgct tggagaaagt gcacaagtac atgaagcatc tgttcagatc
    24961 attggggcga cgactgaatc ttcagaaagt ttcttatcta cgttcaatcg acggattcct
    25021 atggcaatca ccttgccgaa tcttgcttca cgttcactgg atgagcgtta tgaaatcatc
    25081 tctcttttta tcaaacaaga agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa
    25141 gcgattcttg ccttcatgct ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac
    25201 ttgaaactag tctgtgcaaa atctttttta cattaccgta ctcatcatga gaaaaagcta
    25261 atcattcgaa aagaagaact ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa
    25321 ataccggaac gtctcgaccg attcatggac agcaaaagtc agtatttgac atttgaacca
    25381 ggatttgctg atgttgtctg gtcacaagat ccagaacgaa acatgcaggt ctataacgat
    25441 attgaggaaa aagtcacttc tctttccgaa accggtgtgg agaacattga tctagagacc
    25501 ctcatttcaa aagatgtgga tgcttatttc cagacatatg tcaaagaatt gactaaaagt
    25561 tcgattccca aagatctttt gccagatgaa atctggcggt taaccaatca attgtatgat
    25621 gtggctgaaa aacggttaga ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg
    25681 catctgcaaa gtacattgga tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg
    25741 aataacgtac ggaaaaattt taagagggaa ttccaggtag caatcgatct ttcaacgatc
    25801 attgaagaag aagaacaagt tgagattcct tttgatgaaa tcggctttat tagtatgttt
    25861 ctttctatca atctaggaga aagtgagcct gttaaagaaa acaaagtaga tgttctcata
    25921 ttgatgcacg gcagacagac tgcttcaagc atgttggaga tggcacaaga actattagga
    25981 acaaaagtag ggaaagcttt taatatgcca ctggttatgg aagttcaaac aatgtatgaa
    26041 gaagtgttac aatatgtcaa aaactatcaa gagcatttga cgaatggcct actcttgtta
    26101 acagatatgg gatcattaaa caccttcagt gatttgattt atgaagagac tggcattcgg
    26161 acgaaagcta tcacgatgac aagtaccatg attgtgatag aagcgatacg gatggcaagt
    26221 gttggaagaa gcttagaaga tatttatcaa aatatccagc tttcctttga atcgatcgta
    26281 cgtgatcagt tcaaagcgga aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg
    26341 acatgcttta ctggtgaagg agtagctgcc aaattatatc agcgtattat gccagtgatt
    26401 aatcaaaata aggtagaact gatccagatg cagtttttag aacgtgaatc attcaaaaaa
    26461 cacatcgatg ccttattgga agaatatgag atcaaagcaa ttgcgggaac agttgatgtt
    26521 gattatcaaa atatcccatt tttttcagcc tatgacgttt ttgatgatga aaaactaaat
    26581 attttaaagc gtatagctga tgatgagatc ccgacagcta aaattgtcga ttcacttgct
    26641 ggatcattaa gcgatgtctt ttctattgat gaactgatcc gttcattgca aaagacagtt
    26701 cagcagatcc agaccgattt gtcgattatc gtggaacctg gagtagatac tggaatcgtt
    26761 atccatttag ccttcatgtt agacggattg atcaaacaag aaagccatcg gcaatttgag
    26821 aaactgtcta ttttctcaaa aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg
    26881 atggcactag aacgaaaata tcaggtaaaa attccagaag atgatatcgc atttcttaca
    26941 caaatgtttc ttgaaaataa aataaaaaaa tgaacacact aatcatactg tttacacact
    27001 aaacatctgt gtgcgcacta taaaaaagag ctaaaaagca tgaaaacgct atttcagctc
    27061 ttttttttat tggcatgctt cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt
    27121 aaaaatggca aaaaaaacaa tcatgttagt ttgttcagca gggatgagta cgagtttatt
    27181 ggtcacaaaa atgcaaaaag cagcagaaga aaaagggatg gaagccgata tttttgcggt
    27241 ctctgcctca gatgcagata ataatttaga atctaagaat gtcgatgtat tattattagg
    27301 tcctcaagta cgtttcatga aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc
    27361 attagatgtc atcaatatgc aagactatgg catgatgaac ggagaaaaag ttcttgcaca
    27421 agcagaaaat ctaatgaaat aaatcaagta tgcagttggg ggtataacaa cgtggaagat
    27481 caacaaaatc tagaagccat catggggtta atcatgtttg gcggtaatgc aaagagcgat
    27541 gcaatggaag cgattgcggc tgctaaaatc ggtgattttg agttagcgga taggaaaatc
    27601 gctgatgcag aagaatcact tatacaagcg catcatgcac aaacagacat gttgacacaa
    27661 gaagcacaag gaaatcatat gcaagtgaca ttattgactg ttcacagtca agatcactta
    27721 atgacatcta ttgcgtttac tgatttagca aaagaaatca tcgaacttta tcgtcgcgta
    27781 tacagcgaat aaatacaact tcttttttga acggaagaag aaaaattttt gaacggattt
    27841 tttattttgt tataaggaga actctcttta tataaaaaac ttataggagg aagtaaaaaa
    27901 tgaacggact atcaagttgg atggagaaat acatcctgcc ggttgccgca aaaatcggag
    27961 cgcaaaaaca tttagttgct ttgcgtgatg cttttatcgg cacactgccc gcaacaatgg
    28021 cgggatcagt cgctgtaatg atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca
    28081 gtacctatgc tgacagttta gcagcagaca aaggaatctt tgccgtattg aatgtcatca
    28141 tcggaatcaa tggttttgtc tggaacggta cactagcaat cgccggattg atctttgcct
    28201 tttcttgggg gtataatctt gcccgagctt atggagtaaa tgatctagct ggggggatcg
    28261 ttggtttagc taccttgatt caaggtattt cctttggtcc agcattatca gcagcattag
    28321 atgtgaatgt tccttcaaat gtcgcagatg caatcaatag ttctgagatt ggtgcaacga
    28381 ttgcagatgg cacactgtca gttggattat ggggttggct aaaactagat catttgaatg
    28441 gaaacgctta ttttacagtt atgatcatgg gagctatttc agtcattatt ttttgtaaat
    28501 tgatgcttgc caacattacg atcaaaatgc ctgattcagt accaccagca gtatctaaag
    28561 catttgcagc cattttacca gcgacgatcg ctttatacgt cattgctatt atcaattata
    28621 tcgtaggtcg tgtagctgat gggcaactga tcattgattt ggtgcaaaaa tatattgcag
    28681 agccattcct aggattatct caagggttgg gagcagtatt gatcgtcaca gtatttgttc
    28741 aagtattctg gttctttggt attcatggac caaatgtatt ggcaccagtg ctagagggaa
    28801 tctggggaca agcacaacta atcaatattg atattttcca aaaaggttat aacggaaaaa
    28861 ctggtacacc agccgtgtta gatgcaatcg aagatggaaa agcttatatg tgggtccgcg
    28921 gatcgttcga tgcttttgca tggtttggcg gatcaggcgg aacgatcgtc ttactgatag
    28981 ctatcttgat tttctctaaa cgtgcagatt atctaacagt agctaaatta tccattgggc
    29041 ctggtatctt caatatcaac gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa
    29101 tcatgtttat cccattcttg attgctccag tagtagcaac agcaattgga tggacagcta
    29161 cttatcttgg cctagtcgct ccggtatcgc agcaagtcac atgggtagta ccgccgttct
    29221 tattgtcatt cttagcaaca ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta
    29281 tggtcgtgac tttcttgatt tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg
    29341 ccgcaggcga acaacaataa gatgaacgtg aggagcggga cgctttaagt ttcgttcctc
    29401 ataatgaatt ttcgaggagg aactgaaatg attacaattg ccagtgcaat gcaacaagat
    29461 gaaatcaaag aattgttaga aggatacaat gaagaaggaa tgacttttac atttaaagaa
    29521 aaacaaggaa tcaagctgtt ctttgaaaca agtgcagaag atccggaagt tgcggctaaa
    29581 aaagcaaagg aactaatcaa agcacagcct tggggaacag tcctttattt tcaatcaaca
    29641 gctgtttaga aaatggttta aaaagatagg atggttgaga tgataaaaaa cggtttattt
    29701 ttatgtagta ttggttcttt gatcgtgttg tatagtttga atccacattc gcaaaggtat
    29761 gatttgtata gtatggtcac aggtcttttt ctaacaggat taggaatcta tttcttcatg
    29821 aaggggaaaa aaagagaaga agcaaaggag aaaaataact aatggaaatc acgaaaaaat
    29881 taaatattcc agcatcgttt ttttacgata aagttatgga ctctgtcctt ttcgatgtac
    29941 gtaaagcgac cggcaaatca gtaactagaa aacagctgaa aaatttggag tacgtcaaac
    30001 aattctcgca aaataaccgg gctcgcataa aaatcgaaga agtcattgaa aaccagtctt
    30061 ataagtttcg tacatcgaca acgaagaatg aattcgtcgt tcattatcaa atcaaaccat
    30121 tggacgattc gacctgtgag gttcattaca cagaaaaaat ggagtcttat ggcatgcttc
    30181 aaaagttgaa tgatatgctt ttaggaacaa tcttgatgta ttttaaacgg agacgtttca
    30241 aaaaaatgct agatatgatc gaagcaacgt attaaatcaa tgaaacatgg tactatttag
    30301 ttattgaaaa atgaagtaag ctagaaggaa tttttcatag gaagatagat ctttcctgcg
    30361 aatcctcact agcttttttg taggaagggg cagcaaaatg acaaacaaag taagagagct
    30421 aggtgtatct tcgattgagg aaatgtttga tttagccact tatgcattta atggggcaga
    30481 tacacctgaa aggcgagaac gttttcgtac acttgcggag aattcttgga attacggatt
    30541 ttttgatgag gaggatcggc taacaagtca agtcatggcg accccttttc cagtgaattt
    30601 ttacggacaa gagtacctaa tggccggcat tggctatgtt gcttcttatc cagaagcaag
    30661 gggacaaggc gggatcaatc agatcatgga aaagatcctt gaagattgcc gtgatcgtaa
    30721 agtaagctta tcttatctgg cgccattctc ctatcccttc tatcggcgct atggctatga
    30781 acaatcattt gataaaatca gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa
    30841 aaaatctgga aaaataaagc gtatggattt tgaagatgca aaagctgcaa tcagaagaat
    30901 ttatgaacag atgccggaaa accaacgagc tggtctgaag cgcgaagact ggtggtatac
    30961 gtataaattc aagcaaaaag gcaattacca gtttgctttg cattttgatg ataatgagga
    31021 agcaacggga tatatcgttt atcagcttgc aacgtcttcc tttattattg cggagtgggg
    31081 atttttggat catgatgctt tttgcagcct tgttcgcttc gtaagttctc acaatggtgc
    31141 ttttgaaaca tttgaatatt catgcggcta cggcggtaat gatcaaaatt atctattaga
    31201 aaatccattt gcttctgtaa tgatcacacc ttatatgatg gcgaggatcg tagatatttc
    31261 tttgtttttg gaaaattatc catttaaaaa gagacaagct gcatttgccc tacaaataga
    31321 agaagatcat tatgcgaaat ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa
    31381 aaatcatctc tcaaaagtag aacaaacaaa actcccaatc gttcgaggaa gtatccaaca
    31441 tatcacgcag ctattattag ggtatcgtaa agtggaagag ttggtattcc aagaaagaat
    31501 acaggtgaca aaagaatatg aagaaacgtt aagctcaatc ttgccacagc aacagccaat
    31561 tcttaatgac tatttttgat aaaaaacggg tgatgacaat atttttgtca ccaccctttt
    31621 gtttttcatt tggcatttga aggaaaatgt taaagtaaat cctctttttt cgtaaatttg
    31681 aattttttgt tttcttttgt tttagggttt tcttttttgt ggtcttggat tgcttctttc
    31741 gtaatctttt cccgattttt tttagatgaa tctaagcgtt ctgcgtatac tttttctttt
    31801 ggcttatcat tcattggaaa ggacctccct acattatttc attctacctc tagtttatca
    31861 tctgttaagg actagtggaa acgttttgcc taacttataa gctgattttt ttagcagata
    31921 agcttgcaag taaaaaacaa acaagctaca attgatttat tgaaattgtt cctgtagctc
    31981 agtaggatag agcaatcgcc tcctaagcga taggtcgctg gttcaattcc agtcaggaac
    32041 gtacaaataa cccctgtatc ctttgcggta cagggggtta ttttgtataa gtagacattg
    32101 tagtttctaa actgctatgt cctaaacgtt tcgatacgct aagtatattt actccttgat
    32161 aaagtaaaat agatgcatgc gtatgtctta gtccatcaat aaacagtcga agagaaaata
    32221 gggaaaaatt gattactaaa gaaaaactta ctaggtcgac ggtgtagttg attttcatac
    32281 ttcaatagat tcgaattaaa cgatttcgat tggcttatac cagtcctatc caacgaaaga
    32341 tatatcgttc ttaactctag cggtataact tgaagcttta cgtaaagcca atgggaatga
    32401 actggagatg actttttatt atttttagta atagctgatg ggttaatact tacaactttc
    32461 cttttcaaag taccatataa aaaaacggtg gggttgttta gcgaaataaa ggatctaagc
    32521 caatagctta gatcctttat tctatgcttt atattatttt ttgcatgtgt gcgaaagtat
    32581 actcaagccc aatcgccatt tcggaaaatc ggtactgtag agccatcttc acggattccg
    32641 tcgatatcca tcttgtcaga tcctaccatg aaatctacgt gggtttgact tctattcaat
    32701 ccagcttctg ctaattcttc atcggacatt tctgtaccgc ctttcacact gaaggcataa
    32761 gcagaaccta gtgctaagtg attcgatgcg ttttcatcaa ataatgtatt gaaaaagatg
    32821 atgccagatt gagaaatagg tgaagggtca ggtacaagtg cgacttctcc taaacgacgc
    32881 gcaccttcat ctgtatcaag cagttttgct aagacatctt ctccttgttc ggcagaaaaa
    32941 tcaacaactt ttccatcttt aaaagtaaat ttcatacccg agatgatggt gccagcataa
    33001 ctcagtggct ttgtacttga aatgtaacca tctactcgat gactgtcagg tgctgtgaac
    33061 acttcttccg tcggcatatt tgccatgaat ttttctccac gagcattata gcttccagcg
    33121 ccttcccaaa gatggttttt aggcaatccg ataatgatat ctgtaccggg agcagtatag
    33181 tgaagggcag aaaattgttc tcgattcaat tcttctgctt tttttgctaa tttttcatca
    33241 tgcttcttcc atgctaagac agggtcttct tcatagacac gagtcgtttt gaaaatctga
    33301 tcccataatg cctcaacttg tttttcttct ggcagttctg ggaagacttt tgctgcccat
    33361 tgtttaccag ccgcagccac gacagtccaa ctgactttat ttgcttgagt tgccttgcgt
    33421 aaattcatta gtgctttgcc attcgctgat tgataagacg ctactcgatg gctatctaca
    33481 ccagcaaatg catcgggatc ggcagaaaca acgctgatcc tgcttgctcc tttttcaagc
    33541 cattcatccg cttgatcgat tttagattgt ggtacattct caatacgatc cgttgcagca
    33601 tgtaaaagaa attctctttg gatctggtcg tcagtccatt gaacgataac ctcagcggct
    33661 cctagtttat aagcttcttg tgtgatcaat cgagcaaggg gtgcctgttc cacgctgatc
    33721 tgtaaaacaa ctgtgtgtcc tttttctgtg gctacgccag tttctgcaat taagcgtgca
    33781 tattttttta gaagttcatt aaaatcagat aacataataa aaattcctcc tcaccatttt
    33841 ttctggtaac tattagcatg ataccattta attatgattc aaacaatgag gctttaaata
    33901 aaaaaaggag gttatttttt taaggttaag tttcatgata tatgttacta cttgctattt
    33961 cagcttgtga agaaaatcaa aaactaattt tgcatatcaa aaaaacatgt tacaatgaaa
    34021 ttaatcaatg gtggaagcgt tataaaaaaa ttaaggagat taaaaaatat ggcaagaaaa
    34081 aagacgatta ctagagaaca gattttggca gctgcttatg aagtagctgc caccgatgga
    34141 tttacacgat ttactgcgag aaacatcgcg aacaaaatga attgttccac gcaaccgatt
    34201 tatcttgagt ttaaaaatat ggatgacttg agacaagctc tttttgagca gatctatgat
    34261 catttacaat atgaagtgtt cactgttgag catacaggga atacgattat cgatcttgct
    34321 tttaattata tccattttgc aagaagagag aagaagttgt atcgttcact gtacttagaa
    34381 gaatatggtg gaggaaaaga gatgcagaat ttctcttata cttactttgt gaatacagtg
    34441 aaacgcgatc cagcttatgt gaatctttca gaagagcaga tcgaatcttt gtataatggg
    34501 acatggatca tagctactgg gatcgcagct ttgatgtctt caagcatcat ccatccaact
    34561 gatgaacaga ttgaaaagat gatccaagac agtatagatg ctatcttgaa attcaaagaa
    34621 ccaatcgaaa tcgattgaat atactcaaaa gttccacaaa tcagtaggta acaagagatg
    34681 tgtcaaaata aggaaaagtt cgtttttctt acagcgtatg catttttaac tcccgaagaa
    34741 ctcttccatg aatgcaatga ataaaagagc catacagatt gaataaatct ggatggctct
    34801 tttattgcaa gtatattaaa tttttgcgac tagttcttta gcgaaagctt ctaaattttc
    34861 gatatcttct tcttcagcat tcagatccac tttaacattt tcggctcctt ttgatgcgcc
    34921 tgtttttgca aaagcagcat cgaaatcatc caccgatttg cagaattcat cgtaaaaagt
    34981 atcaccagat ccgcagacac cgtaaatttt tcccaataga tccaattctt gcaagtcttc
    35041 ataaaagtct acgatctcat caggcagatc gccatcatcg tatgtataag tagctactac
    35101 gcaaatatct gcctcctcga attcatctgc atctacttgg gtacattcgt tgatttcgac
    35161 ttcgatatcc agattttcaa atgcttctgc aacaatatcg gcgatttctt cagtgtttcc
    35221 tgtcatgcta gcatatacga tttttgctaa agccatgatg tgcctcctcg tttataatta
    35281 tttaacaatt atgaattata ccaaaacatc attgaaaaga aaataaaacc taaagacacc
    35341 tggggaatgt catttggcta aatgtatgcc gctatgttaa aatgagaggg atttgtaaaa
    35401 aggagacgaa aatatgatta ctttaaaatc agcacgtgaa attgaaatga tggcagaatc
    35461 tggagcttta ttagcagatg tacataaaaa tctgcgtgat tttataaaac caggaatcac
    35521 aagttgggat attgaggtat ttgtcagaaa ctatatcgag agtcatggcg gaattgctgc
    35581 gcaaattggc tttgaagggt atgaatatgc gacttgtatc agtataaatg atgaaatttg
    35641 tcatggattc cctcgtaaaa agcctttgaa aaacggtgat ttggtaaaag tcgatatgtg
    35701 tatcgatttg aaaggcggca tttctgattc ttgttgggcc tatgtagttg gagaatctac
    35761 acctgagatc gaccgattga tggaagtaac gaagaaagct ttgtacatcg gtattgagca
    35821 agcacaagta gggaatcgga ttggtgacat cggtcatgct atccaaactt atgtagaagg
    35881 cgaaaatcta gcgatcgttc gtgattttat tggtcatggc gttggtccta cgattcatga
    35941 agaaccagca gttcctcatt acggagaagc tggaaaaggg ttgcggttga aagaaggcat
    36001 ggttatcacg atcgaaccta tggttaatac cggaacttgg aaaatgaaaa tggatcctaa
    36061 cggctggact gcgtatacaa gagacggcgg actttcttgc caatatgaac atacattggc
    36121 tattactaaa gatggtccaa gaattttgac atcacaagga gaagaaggca cttattgatc
    36181 tatccagtca ataggtacgg gagaggtaca tgaaagtttg gaacaaaata aaacaaaatg
    36241 aaaatctgat gcgctttatt gagacgactc aaaagcgcat cactgattca gaaatgagta
    36301 cgacctctgt cgtcgttgct tattatttac tcttgtcttt atttccattg attattgctt
    36361 ttggtaatat tttaccgttt ctccacatcg atcaagagac tgtactgacc tatttacgag
    36421 aagtgattcc tgagaccgtt taccagttta tcggtcctgc aatcaaagac cttttgacac
    36481 aaagttcagg aggcttgtta tccgtttctg cattagcggc tttatggtct gcaagtcaaa
    36541 gtatcaatgc tttgcaaatt gcgatgaata aagcgtatgg cgtagaaaat agaaaaaatt
    36601 ttttgatcgt ccgtttcttc tcactaattg tcattgtctt gttcatgatt gctattagtg
    36661 gggtaactat cgttcttggg ttgggacagc tgattttgga tgctattcag ccaatcgtac
    36721 aaatacccgt gaactttatc gatcagttcc aaactttgaa gtggccaatc acattggtag
    36781 ctttatttgt catcatgttc ttgatttatt taattgtccc taatgctcag ttgaagttga
    36841 agacgatcat tccgggcacg atcttcgcta ctgtcggctg gatgctgtta tcacaagtgt
    36901 ttagtatcta tgctaggtat tttgcaacga ggatcagcgg ttatcagatc ataggaagtt
    36961 ttattgtact catgctttgg cttaattttg ctgctacgat catcattttg ggcggaatca
    37021 tcaatgcagt tgttcaagaa tacgtgacgg gtcatcaaat caaaaaacgt cgagatccaa
    37081 gccgaaaatg gttcaataaa ttgaaaacta aattttctca taaaaaaaag tagaaacaag
    37141 agttcttatt gtaaatttat atcagtaatt tataataatt atgactctta attaaagtag
    37201 gtgcccatat gcttgtttct tttagtatgg ttataagatt ttttctgaca attttattat
    37261 cactgatatt aacacctgta ttcaaaatca tttcagtaca aacaggtatg gtcgacaaac
    37321 caaatgaaag aagaataaat aaagttccaa tgccttcagc gggcggactg cctatcttta
    37381 tttcatttgt tatatcgact ttattcttat tccgtaaaat tatccctcaa tcgtatattg
    37441 taccgatctt gattgcttct ttagtaatca tagtcacagg agtgctcgat gataaatatg
    37501 agctgacacc taaacaaaaa agtataggga tcttgattgc ttctcttatt atttattttg
    37561 tagcagatat acgtattgat tccttcacgc ttccttttat tggatatatc caactaggct
    37621 ggcttagttt tcccgtaacg attttttgga tcttcggtat cacgaatgcg gtcaatctga
    37681 ttgacggttt ggatgggctg gcggcaggga tttcattgat cggtttaatt acgataggaa
    37741 tcattggata ttttttcctc catgcttcta cagtgtatat tccagttgtt attttctgtc
    37801 ttgtagcaag tattatcggt ttttttcctt acaattttta tccggcaaag atttatttag
    37861 gcgatacagg agctctattc ctagggttta tgatggccgt cctttcacta caagggctga
    37921 agaacgtgac ttttgtttca tcgatttctt tgctgatcgt catgggagtg ccggtaacag
    37981 acacgttttt tgctatcatt aggagaaaag caaaccgtgt ttctttttct acagcagata
    38041 aaaaacatct tcatcaccga ctaatcagtt taggctttac tcataaaggt gctgtcctga
    38101 cgatttatgc gatggctttg atgttttctt tcacagctat ggtcatgaat tatactggaa
    38161 gaatcggaac gatcgtattg attattgcca tgttgtttgc tgcaatttta ttatttgaat
    38221 tgattggtct gatcaatgag aaacatcagt ttattttaaa aacattgcga tttttaggga
    38281 ataaagagta ccgtacgcaa gtaaaacaga aatatttgaa gaaatggaac cgtcatatag
    38341 atgaataaca aattaaacca gccgatcctt attagtattg tgacggcaaa cagtgataaa
    38401 atttttcgga cactagataa ttttatttca actgtaaaag atgaaacagc agtaaaaatc
    38461 cgaatttttg acaacaattc acttcccgag tacaaaaaaa gactaacaga gtataggaaa
    38521 tatccctaca tcgatatcta ttttcatgat gaaaattccg gatttggtta tggacataat
    38581 cacaatctga tagctagcga ttctcgctat ggcattattt gtaacccaga tattttagtg
    38641 gatgaagaga cagttacagc attggtcttg ttattgaaag aacatccgga atgtgcgatg
    38701 ttagcaccta agattcttaa tgaagatgga acaactcagc acctcatccg tgagcagctg
    38761 gctgtttttg attacgtttt gcgctttgtg cctttcaaat gggtcaaaaa aaagtttgac
    38821 aaacgactgg ctgtgtttga atgccgagga ttgcctactg atgctaatag ttatgtacgg
    38881 atgatctcag gtagttttat ggtagctgat attgataaat ttaaagagat aaaggggttt
    38941 gatgagcgtt tcttcatgta ctttgaagac aacgacttgt gtttgagttt tgaaaaagca
    39001 ggttacaagt tattgtacac acccttttat gcagttatcc atttttacgg gaagggtgcg
    39061 catcgaaatg caaaactgtt tcgcgtgttt ttgacttcta tgatgaaatt tttcaataag
    39121 tgggggtggc gttttttctg atgaaaccag aaatattagt agtcgttgtg ctttatcagc
    39181 aactattcag tcaatctcca gcttatggac cattaagcaa agcacttagc gaaaagcaaa
    39241 ttcagcttat tatttacgat aatagtcctc aaaggcagca aaatgagctg tttgaaaaag
    39301 aaaatatcgt ttatcatcat gatccgcaaa atcctggttt agcggtggct tataattttg
    39361 cgttgtctca agcagatgaa ggaacacgtt gtctcgtcac tctggatcag gatacgaggc
    39421 tagtcgacga ttattttgaa ctattgcgta aagtaacctt tacagatgaa tgtgttgctg
    39481 cagttccgat gattttttct gggagccgac aagtttctcc cctatattca gatcgttata
    39541 tcaatcgtac agctcaagca gtagaagttg aaggcccaac agctgaacga atcatggcaa
    39601 tcaattcagg tgttgcctgg tctgtcaaat ttctaaaaaa gattggcggg tttaatcctg
    39661 ctttttcttt agattttttg gatcattggc tgttttggaa agtggatcag ttggaaaaaa
    39721 ctgtggagat ccttcctgta cgaatggaac atgatctttc ggttttagac tatgaaaaag
    39781 tttcaagtaa tcgataccgt tctattttac aagcagaaaa tcgattttat catgaatatg
    39841 atcaaaggca tttatccagt caccgacgac aattattgtt aagaacagct aaacagttct
    39901 tgaccgtcaa aaaccggcat atctggcgga tgactttgcg ctcatatgtt aagaattgga
    39961 aggtgtgaat tcgtgcgttc ggtttgtata gctaccttta atggaggcag atatttaaga
    40021 gagcagttag atagtatttt atctcagctt tcagcagaag atgaagtagt catttctgat
    40081 gatgggtcag tagatgatac atgggatata ttaactgaat acgcagaaaa agataagcga
    40141 atccagcttt tttcggggcc tcgtcaagga ttgatcgcta attttggtta tgcgatccag
    40201 cagacgaaag gagatctaat ctttttggca gaccaagatg atgtttggtt ggagacaaaa
    40261 gtgaaaaaga tcactgacta ttttgagcag aatccggatc agctagtagt agtttctgat
    40321 ttaatcattg tggacagcga gctgcaagag atccatgatt cgtatttttc gtttcgaaat
    40381 tccaaagaag gatggcttaa taatgtgata agaagtcact atattggtgc tggtatggct
    40441 ttccggtctt ctttaaaacc cgatattttg ccgatcccgt cagaagtgcc gatgcatgat
    40501 atgtggattg gtttgctggg aggaaagaag actgggttcc taagagaacc gttaactctg
    40561 taccgaagac atgatttaaa cgcgagtgag attgaaacac attctaaatt acggcaaaaa
    40621 atttcttggc gtgttcatct gattacggcg ttgatcaaac gaaaatggtt taaacgttga
    40681 taaaagatgt ttaaatcctt aatatttcca tgacaaatat aacaattgat gagataataa
    40741 caaagtgaaa tttgaaatca aaaaggagag cagatatcca tgaagggaat cattcttgct
    40801 gggggcagcg gaacacgtct gtacccttta acaaaggcaa catcgaaaca attgatgccg
    40861 atttatgaca aaccaatgat ttattatcca atgtctactc tgatgttggc gggaatcaaa
    40921 gaaattctga ttatttcaac gccacaagat acaccacgtt tcaaggaatt atttggaaac
    40981 ggaaatgatt taggactgca gatcgaatat gcagtacaag aaagtccaga tggattggca
    41041 caagcgttta tcattggaga agaatttatt ggagatgata gtgtctgctt ggtattaggg
    41101 gacaatattt actatggtgg cggtctgtct aaaatgttgc aacgagcagc ttcaaaagat
    41161 acaggcgcga cagtatttgg ctatcatgtc aatgatccag aacgctttgg tgtagtagaa
    41221 tttgatgaga atatgcaagc tttgtcgatt gaagaaaagc ctgctcagcc aaaatcaaat
    41281 tacgctgtaa ctggccttta tttttatgac aatgaggtaa tttctatcgc taaaggtatc
    41341 aaaccttcag aacgtggaga actagagatt acggatgtca ataaagcata tttggataaa
    41401 ggaaaactat ccgtagaact tatgggacgc ggatttgctt ggcttgatac aggaacacat
    41461 gaatcattgc ttgaagcatc tacctttatc gaaacaattg aaaaacgtca gaacttaaaa
    41521 gtggcttgtt tggaagaaat tgcttaccgt atgggctaca tcgataagga acaattgctg
    41581 gcccttgcac agccattgaa aaaaaatcaa tacggtcaat atttattgaa tttggccgct
    41641 gaataaggag tccttacaga tgaaagtaac agatacaaaa ttaacagatg tcaaaatcgt
    41701 agaaatggac gtctttggcg atcatcgcgg tttcttcaca gaaagttatt caaaagcaaa
    41761 atttgcagaa catggattga aatatgactt tgttcaagac aaccattcac tatcagctga
    41821 agctggtgtc cttcgggggc ttcatttcca aaaaggagaa gctgcacaga caaaattgat
    41881 tcgggtagtc actggagcag ttttagatgt agtagtggat atccgcaaag gtagcccgac
    41941 ttatggtcaa tgggacggat ttatcttatc tgaacataat catcgacagt tgcttgtacc
    42001 taaaggatat gcccatggat tcgttacttt gacaccaaat gtgaacttcc tttataagtg
    42061 tgataattac tataatgcag aagcagatgg gggcatcgca tttgatgatc cagatttagc
    42121 gattgattgg ccaattgatc catcaagagc gattatgtcg gaaaaagatc accgtcatcc
    42181 gacattaaaa gaatttgaag cagaaaatcc atttgtttac ggagaaatct aagaattaag
    42241 gagaaatttt cttatgaaaa acatcattgt taccggaggt gccggattca tcggctctaa
    42301 tttcgttcat tacgtggtaa acaatcatcc agaagtgcat gtcactgttt tggataaact
    42361 gacttatgca ggaaacaaag agaacttggc cggacttcct agcgatcgcg tagaattggt
    42421 cgtaggagat gttgcagatg cagaattagt ggaccgtcta gtaaaagaag cggacgcagt
    42481 tgttcattac gcggcagaat cccataacga taattcatta aaagatcctt tcccatttgt
    42541 acaaacgaat atcattggta cgtatacact tttagaagct tgccgcaagt atgatgtacg
    42601 ctatcatcac gtatctactg acgaggttta cggcgatctt ccattacgtg aagacctgcc
    42661 aggacatggg gaaggcgaag gagaaaaatt cactgcggaa acaccatata atccttcaag
    42721 tccatattct tctacaaaag ctggttcaga tctgttagta aaagcatggg tgcgttcttt
    42781 tggcttacgt gcaaccattt caaactgttc aaacaactat ggtccttatc agcatattga
    42841 aaaatttatt cctcgccaag taacgagtat cttaagcggt attcgtccaa aattatatgg
    42901 cgaaggaaag aatgttcgtg actggatcca tacgaatgat cattcttcag cagtgtggct
    42961 gattttgacg aaaggcagaa ttggcgaaac ctatctgatc ggtgcagacg gtgaagaaga
    43021 taataagacc gtgatggaat tgatcttaga aatgatgggc caaccaaaag acgcatacga
    43081 ccatgtaaac gaccgcgcag ggcacgattt gcgttatgca atcgattcga caaaattgcg
    43141 agaagagcta ggttggcagc ctgagtttac gaacttccgt gatgggttgg ctgacacgat
    43201 caaatggtac gaagaacatg aagactggtg gaaaaaagaa aaagaggccg ttgaagcagc
    43261 atatgccaaa aatggacaat aatacagagg aaatttcact gtattaatgg aaaaacacat
    43321 gtgaggcagg cgctcgcctg tctctttttt aaaataaaaa tcaaggagcg gataaccata
    43381 tgtttttatt aactggagga aatggacaat taggtacaga attgcgccat ttattagatg
    43441 aaaaaaatct gccatatgtt tcaacagatg caaaagactt ggatatcaca gatgctgaga
    43501 aaacaatggc ttatattaca gaattaaaac ccgatgttat tttccattgc gcagcttaca
    43561 cagcagtaga taaagcagaa gaagaagcaa aagaattaga tgagaaaatc aatgtcgatg
    43621 gcacaagaaa tgtagcactt gcagcaaaag ctgtgaatgc acaattcgtc tatatcagta
    43681 cagactatgt tttcgatgga aaaaacaaaa atgtagaata cgaagtagac gaccaaacga
    43741 atccactaaa tgaatatggt cgtacgaaat tattaggcga aaaagcagta gaagagattt
    43801 tagaagacta ttatatcata cgaacttctt gggttttcgg gatctatgga cacaatttcg
    43861 tcttcacgat gcaacgccta gcggaaaccc gcgacaaact gactgtagta aatgaccagt
    43921 ttgggcgacc aacttggaca cgtactttgg cagaatttat ggtatatgtc atcgaagaaa
    43981 aagcaccatt tggtatttat catttgtcga acgaaaatag ctgttcttgg tatgagttcg
    44041 caaaagaaat attgaaagat acagatgtag aagttgcacc tgttacgtca gaagaatttc
    44101 cgcaaaaagc aacacgtccg cagtattcag tcatgagcct gaaaaagaca gaagcgttag
    44161 gttttgtcat accaacttgg caagaagcac ttgcgcaaat gctggaagct gccaaaaaaa
    44221 tgaataagta atttcctttt tatcaaatag aatttggagc atgatatgga aaaacaaaca
    44281 agaagtgcac gacgaaaaga aaagccaaaa gcgaatcagg cagattcgaa tttggtgctt
    44341 gacctgtttc taaaactccg ctatcttatt ggcgttttag tcatcgtggt catcgttact
    44401 tttaatttaa acggaagttc acttggtgtt tgggataaat atgtgtcgca aagagacgat
    44461 gggaaaaaaa ctgatgtgat ttttggacag aatcgggaag tgcggtcaga cgaatggctg
    44521 gtgcagacac ctttttacct ctcacaagct gaagaggact tttcgttggt gaacgaggat
    44581 tattcattaa acggacaaaa catgatcatt gcgtataatt ctcctgtaaa ggatattacg
    44641 gttatcggaa aaccattcaa ctggggattc tttttcctcg gaagagatcg aggactttcc
    44701 ttttattggg cgatgaaatt agtcgtcatg gtcttgcttg gattcgaagt actgatgata
    44761 ttaacgaaga ggaaaaaagc tctatcgcta atcggtgcat ttgcgctgac attttctcca
    44821 gcagtccagt ggtggttcat gcaacatgtg ggcgatttga ttttctttac tttgggactg
    44881 atgattgctt tttatcatta tttctacaat catgataaaa aatggctacg aacgttgatg
    44941 atgcttttgg tggttatttt cggtattggg tttattttag tgatttaccc agcccatcaa
    45001 gtgatgctgg cgtacttgct gattttttat tttgttggcc tgcttattta ctatagcaag
    45061 aaagtttcat gggatctgtt ggatgtagtg ttgattggag gggcagtttt atttattggc
    45121 ggagtcatgg ttcatttctg gctaacgtca aaagacgcat tgttagcttc tttgaatacc
    45181 ttatatccag gaaaacgggt aagtactgga ggaaagtgga cgattggcga atttttttct
    45241 ttccttacaa attggaaaat tccttttgaa gatatcaact attcgaataa ctctgaagtg
    45301 gccctgttct atcacttttt tccaactgta ttcctagcaa gtccatttgt gttatttggc
    45361 aaaaaagata gcgaacaaaa gttactggga cgtattttga tgattttttg tctgttttct
    45421 atcttttgga ttaccgttgg gttgccaaaa tggctagcag aaatcactct gctgtcttat
    45481 gttcctccat taagagccat ccttactttt agttttgcgg cttgtttatt atcgatctgg
    45541 tttatcgctt atatttggag agaagatgtt attcctattt ggtataaatt cattttgttg
    45601 gcagtaaatg catggtctta tttctatgcg attacgcatt cgaaaatgga taattatttt
    45661 tcggattttg aaaccatcgc agttgctgtg ttgggaacag tgattttagc ttttgcatta
    45721 tttggctgga gacgcttgtt ttacctcgct tttgtcgcct tagttatcgt atctggcttt
    45781 ttcgtcaatc cagtcgtaga aggtacagga gcgatttttg aaaaaacact ggcaaaagaa
    45841 atccaaaaaa tcgatcaaca agatcctggt aaagtttggt tgtctgaaga tgaattatat
    45901 aacttcaccc caacattggg agtgaaatcc ttcaatacag tacgctttta tccagatata
    45961 gctgcatgga agaaaatcga tccgaaagaa gaatacgaga agtattataa tcggtatgct
    46021 catacaagag cttttatcgc taaggaagat acaaagttca ttttagatcg tccagatatg
    46081 ttttcggtca cgttgaattt tgaagacgca gaaaaattag ggatcgacta tgtgatcagt
    46141 aaacgaccat taactgatta caaccaatta tatgaagcgc agttcacgca gctatatgga
    46201 ccggataaag acggttatat gatttttaaa gtagaatatc cggattatcc agatgtacca
    46261 caaacacaaa ctcaggaaaa tcagctagtc gtacagtaat aggctagctg agaataatcc
    46321 tgaaatatta gtagaaagga gagtgaagat aattcaagca atcgtacaaa aatttgtcaa
    46381 caaaatgagt gtcggtttct tgcttttgtt gctgacgcta gtcttttttg tttggacagc
    46441 aggggtaggt tttccagcaa ctttgtttat atataacaat agttcattac ttatcgcttt
    46501 tagtgtggct attttgctca ttttaaatgt ttataaagca aaaggcgtcg attttcttta
    46561 tctaggtgtt gcgcttgccc ttttcatttt cttttcttac tttcgggaat taagaaatga
    46621 gtctttattt ggagacttac tgtttccttt ggttatttta ttttgtttag taattaaatg
    46681 gatcgagtgg acaaaatggg atcgcggtat ttatctgatt gtctttacgg tcttcctcgg
    46741 aatcacgtta tatcgtgtat ttacagagat ccaagtacca gaaggacaaa gcatctgggc
    46801 tccaggcaat tcgctttctg atatttggat caatacgaat acaatcggaa gttctttgat
    46861 gactcttgct ttattgatca gtggatttgc cagttctttt gaacgatggt atgttcgaat
    46921 catcggtggg atagcagtcg ttgcagcctt tttaggtatt tgggtctgcc aatctagagg
    46981 tgcattagtc gcgttgattg tttttgcgat attggatctt ttacctaaat cgttgatgcg
    47041 tgtcattcgt gcgccattta ttgcctacac ggttacgatt cttttagctt tgccgatttc
    47101 ttatctggcg gctgtctctg aaaaagtcaa tttgtttacc gatcgagaag atatttggca
    47161 taaattctat cagacgctag gagaaaaatc tgaacaagtt ttactaggaa tgaaaacatt
    47221 tatcttccag cgaggaaacc aatttttagg aaaccacaat agttataatt ctattttgaa
    47281 tatctatgga ttgataggat ttgggattgc tgctttgctg cttatccttt ttattggtcg
    47341 cttaacgttg aaagcagatt tatccaacgg gcagatgact tttatctggg ccttttttgc
    47401 ggtgatgatg cagtcattta tggaggatac attgacttca tacccatggg ttccgattat
    47461 ttatatcctg ttggctatgg ccgttcatcg atatgatgaa ccagaagttg cggaggaatt
    47521 ctatgttatg gaagaggcat atgacgatat gacttatgat gatacatctt atgaggaaca
    47581 accagatagc gctcattctt ccaggatcca acgctatcat tgataggcac aaaatacgag
    47641 agaggctgtg acaaccgtcg cagccttttt tggtttattg caattgtcta gcatcttctg
    47701 taaaatagaa acgttagaga aaatcttgta tcatagaaaa ttaagaaaga aataagaacg
    47761 cagctcggta ccaaaaatat ggtaaaatgg cacttgactg taattcactg aaaatcgctt
    47821 ggaggaacac aaatgtttaa agatttgtat ttatttatga aggacattta tcagaacaga
    47881 aaattattgc tacagttttc gatcaatgac ttcaaatcaa gatatgcagg atcgttttta
    47941 ggaatcttat gggcgtttat caatccgcta tttactgtat tgatttattg gctggtattt
    48001 ggtgttggac tgaaagcaca aatgatcgat gcaaaatacc catttatcgt atatttagtt
    48061 accgggatga ttccctggtt cttcttctca gatgcatttg catcaactac ggttgttttt
    48121 cgggaatata cttatttagt aaagaaagtc gtattcaata ttcgtatctt gcccacgacg
    48181 aaaatcctat ccaatctgta cacccattta ttcttcattc tgattggcat agctgtcgcg
    48241 cttataaatg gtatatatcc ttcagcaatg tctttacagc tgatttatta tttattttgc
    48301 atgtgtgctt tcttgacagg attgacttgg ctgacggcat cgatccaacc atttttacct
    48361 gatatcatgc aattaattac gatcgtgctg cagttgatca tgtggacatt accgatatta
    48421 tggagcccta atcagtttga ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac
    48481 tatgttgttc aaggttatcg tgagtcattt ttaagtgaag cttggttctg ggagcatgga
    48541 acatatactt tgtatttctg ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg
    48601 ttccgtagat tgaaaccaca tttttcagat gtgctgtaag gagaatagct atgacagaat
    48661 atgcaattag actaaaagat atcacaaaat cttataatat gtacgcaaaa ccttcggatc
    48721 gatttcgtga agccctcaat ccttttaaga aatcctatca tgatgtattt tatgcgttga
    48781 aaaacgtgaa tgtagaaata aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat
    48841 cgggaaaatc tacaatgttg aaaatcatta cgggagtact gacaccaaca tccggcacga
    48901 tggaaatcga aggaaaaatc tcagctttgc tagaactagg atcaggattc aatcctgagt
    48961 attctggcta tgaaaatatc tatttaaatg gaatggttct aggattttca agagaagaag
    49021 tcgatgaaat ggtcgatgat atcattgaat ttgctgacat cggagaccat atttatcaac
    49081 ctgtaaaaac ttattcaagc gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg
    49141 tgaatcccga tattttgatt gtggatgaag cattggcagt aggcgatctt gaatttcaac
    49201 taaaatgtat ggaaaaattt acagaaatca agaactcggg aaagacgatt ttattcgttt
    49261 ctcatgatat cagttcgatt cgtcgttttt gtgatcgttc ctattggttg aaaaacggag
    49321 aagtcgtcga atatggcgat acgatggaag taacgacgaa ttatgaaaac tttttaaaga
    49381 aaaaatcagt taaaacagtg gatcggaata aaaaccaagt agaacggttc gccgctccag
    49441 atatcgtcga cgtgacgaaa gcggaattgt tggataaaga caaaaatccg gtggatatgc
    49501 tggaacaagg agaaaaacta tacgttaaag taacttatga agtaaaagat gatacaatca
    49561 aaaatccggt tttaggaatt gcgctacgaa cagtcgacaa tttttatgta tgcggactta
    49621 atactctact agatggtatc aagattcctt ggaaaaaagg agagaatatc ttttatctgg
    49681 agtatgaaaa gttaggtttg ttagctggtg aatattattt tgacgttgcg gtctttgaag
    49741 aaaatgcaac ggttcctttg gtatataaga caaagtacat gaacttgttt gtcagcggtt
    49801 cttatatcgg agaaggcatc gtggtcttag accataaatg ggaagaaggg actcatagca
    49861 atgaaatatg attttgaaat ggacctagat gaacaaagca gtgtgggaaa aattgccgca
    49921 cagatcaaac caggaagtaa agttttagag tttggtcccg gaaacggccg attaacgaaa
    49981 catttaatcg gagcaaaaca atgtgaagtg agcattgttg agttagataa ggaattgttc
    50041 gattttgtca gcgaatttgc tcaagatgga ttttatggtg atatcgaaag ttttgagtgg
    50101 gcaaactatt atgctggaca gacatttgat tacgtcttgt ttgcggatgt tttagaacat
    50161 ttggtagatc ctggaaaaac attgaagaaa gtcagagaat ttttgaatga agaaggagag
    50221 atcctgatta cattccctaa cttggcgcat aattctgtca tgatcgattt gttcaataac
    50281 caattgccgt gggcttctta tggtctccta gatgaaaccc ataattcttt ttatactcat
    50341 gatggcttcc aaaaagtttt tgaaaaagcg ggattattca ttaatattga agattatttg
    50401 tacttagctg taggtgatac agaattaaaa tcaacttatg aagaattgcc ggaagctgta
    50461 cgctatgatt tcaaaatgcg accatttggt gaagtctacc aatacttttt ctcattgatg
    50521 aaacatccag tagcacaaag ttccattgca gagccacaaa attctaatta tgtaaaagtg
    50581 ttagaagtaa cccagcaaac gaaacaagat gaaacaatcc aacaaatacc attcaataat
    50641 ttcacagggg aaaatgagac actaagtttc cccgtatcag aaacaacaga aagaatggtg
    50701 tttcgcttcg cgcaacaacc tagctttatc gaattcagcg cagaagctgc cggagaaaaa
    50761 ctaacattta tcgattcgaa tgctgtagtc aaaacagtaa atgattgtta tttgtttgat
    50821 gggaaagaat taccagagtt cgttctaacg gatatttctg ggaaagaagt gactatccat
    50881 tgtcactatc gctttatcgg ggaattgaca ccaacaatga aagaattgtt ggaaacgatt
    50941 cgtcccatgg ctgaagtcac aaagcagctt tctgagaaaa atgacgaatt agaaagagaa
    51001 aatcatcatc ttcttgagga aaatacaaga ctagaccata cgttaaaaac aaccacaaat
    51061 cgctattgca cattgttgga aagtgatgaa tggacaataa aacatagact aggacgacgt
    51121 aagaaagaaa cttcgaaaaa aatccaagaa aaagaacttt ctatatgtat tgacgagaaa
    51181 atctgggatg cagaaacaaa aatattaaaa atcatcggat ggggaatcgc taattcggat
    51241 cgccagccct tgtcctataa gttatcagct gaccaatcgc catttttcga agcaattcct
    51301 gtttcacgag aagaagtcaa tcaagcagaa cagctcgcaa aaggaacaga agcgggattt
    51361 gagttacgaa tcctttgcga gcaagaacgt tcattcttag tggaagcagt agctcaaaat
    51421 ggccaatcat ggatcattga aatgtaaata atgattaact tataaagata aggagtaaac
    51481 gaatcgtgca aaaaaaagaa attgccgtat ttatcgatca aatcactcgt gaacgggcca
    51541 ctggcgattt gcttattgtg ggctgggcaa ttgatgaagt gaccaaagaa atccctacga
    51601 taaaggtaga aaaagaaaat gtgattgctg aagcaacaca tgtcgtaaga ttggatatca
    51661 atcatttata taatcttgat gttaagaccc aaagcggttt caaggtacgt ttatcaggga
    51721 aaatgagagg aaaagccatc ttagacttcc aaacagcgag acatcaaaat ggcatagctg
    51781 tgaaattaaa cggtaaatac ccttatgatg acggcattga atcttcatgg gaaagaaaaa
    51841 aaagattact gaaaaaaggg ataaattatg caaggactca tggcgtaaaa aaagcgatcc
    51901 gtcgggtcaa attggaattg aagccaggat cgatcgacta tgcagagtgg atcagtcgtc
    51961 atgagaaacc agatttgaaa gagcaaagga aagaagcaca aggatttgcg taccgtccat
    52021 tgatttccat acttatgcct gtctataacg ttgaaatcaa atggctcgaa aaatgcattg
    52081 attcggtact ggatcaaacg tatgatcatt gggaactatg tatcagtgat gatgcttcta
    52141 ctgatccagc tatccgaaag tgtctggaat cctatcaagc aaaagatgat agaatcaaag
    52201 tcgttttccg aaaagaaaat ggacacatca gtctggcaac taattctgca ttggaaatag
    52261 cagaaggaga atttatagca ctcttagata atgatgatga attaccgcca tttgcattat
    52321 atgaagtggc aaaagtgcta aacgtacatc cagaactgga tgtgatctac agtgacgaag
    52381 ataaaatcga tgcagatggc aatcgctttg atccgcattt caaagcagac tggtcgcctg
    52441 atacattgat gggaaataat tatatctctc acttaggcgt atatcgaaca agcattgtta
    52501 aagaacttgg cggtttccgt aaaggctatg aaggttctca agattatgat ttggtccttc
    52561 gtgtgacaga acaaatccct gcagatcata tttaccacat tgaccgagtc ttgtatcatt
    52621 ggcggacgat cccaggatca actgcaagta atggcgaagc caaaagttat atctatgatt
    52681 ccggagtgaa agcattgaca gatgccctgt ctagaagaaa catcaaagga agcgtacgtc
    52741 ctggcagaat ttctggattt tatgagattg cttatgacgt cttgcaagag gatttggtca
    52801 gtgtgatcat tccaacgaag aatggctatg aagatctaaa aacttgtgtg gattcgatca
    52861 tcgaaaaaac gtcttattca aattatgaaa tcattattgc ggataacgga agtacagatc
    52921 caaaaatgca agaattattt gcagaataca agcatcagct gaaagaccgc tttatcgtag
    52981 aattgattga cattccattc aattattctc gaattaacaa tttagcagca gaaaaagcaa
    53041 acggcaaata ctttttattc ttgaataacg atacagaagt gattgagcca gattggatga
    53101 cggtgatggt ttcttatgca cagtttgatc gaatcggctg tgtaggagca aaactgtttt
    53161 atccagatga tacaacgcag catgcaggag tattattagg aatcggtggt gttgcagggc
    53221 atgcattgaa taactatgat cgaacacatt gtggttattt cggccgattg gtcattgacg
    53281 tcaattactt agcagtcact gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg
    53341 ttaacgggtt tgatgaaaca ttagaagtag cattcaacga tgttgacttg tgcttgaaag
    53401 tttatgaact gggtcgttac aatgtctatg cacatcaagc agaattgtac catttcgaat
    53461 ctaaatcaag aggttatgaa gatactccag aaaaacaaag acgttttgct ggtgaaatca
    53521 aaaaaatgca agataaatgg ccagcatata ttgcgcatga tccattttac aatgataatt
    53581 taacaaaaga cggaatcggc gacttttcgt tgagaccaga ttaaactata ataaaaagct
    53641 gttatgttgt gattcgctcg cacataacgg ctttttatag ttttttgtca aaaaaaggca
    53701 gaaggtgccg actcaaaaaa ataagtcgag gttgggacag aagtgtttaa ctccgagaaa
    53761 taagaagaaa ttcacgaaaa ttgcttttca aatttttgtg aatttcggct tatttaagaa
    53821 gaagttgctt ctgctcccac cgtttattcg tttttaaaga atgagacaaa agtgtttctt
    53881 acttttgtcc cacgctctta ttcatgctca gagcgccaat agatacaaaa tcatagccaa
    53941 agtttcacga accctagctg tcaaagaatc gatattttat tttaatatag gctattttaa
    54001 aataaatggt ttaaggtatt cctctacaaa agccactcta tcaattatca tgggtttaaa
    54061 ataagaagtg atggagataa ccagattgtt cttagcgtca atataaagaa catttccact
    54121 attaccaatt gctgaatata tttcgttctc agaatctaat acccaccata aatagccata
    54181 tctcatattt tgtaagtctt ctaaagcact ggttctaggt ttgaacatct cgtttatcca
    54241 catgggtggt acgatttgtt ttcctttata catcccttta tttaggcata atacaccaat
    54301 ttttgccaga tcacttgccg ataaacacaa accatatcct gccgtcccga tatttagtgg
    54361 atcacaaaac cagacattgg ttttaggaga cttatccatt gtaaactgct tatgttcttc
    54421 agctgtttca gcataataat tgacatggtt tggtatccct ataggatcaa atagatactt
    54481 attggcaaaa tcaacagttg ttagcttact tgtctgatac aacaagccag ttaatatgtg
    54541 taaacagaca gtttgatagt tgaattcgcc ggttatacct tttcttcctc ccaataaatc
    54601 tagtgatgtt tttgtccaat cttcacttga acagactttt gtccatggat cgcctttaca
    54661 tttgtagggt gctttcattg ttagcaagtg cttgattgta atagcgtaaa tagttttctc
    54721 cccgcgtttc actttatact ctggaaaata atctaatact ttatcatcga tacttttgat
    54781 taatccttgc tctaaagcaa tgccaatcag caaagataca atacttttcg ttgctgacat
    54841 gatatgacaa gtatcgtctt ttttataacc attccattca tctgtataaa cttctacccc
    54901 atccttatat gcgacaattt gacatatatt tggttgtcga aaaccaataa aatcatgcaa
    54961 ctcttgttta ttcataagat attacctcac tttcttctat aagcataatg tcattaaaaa
    55021 tcgatttcaa ttttttcgat tcttcacgta atacaggtat tcttatggac tagacagcgc
    55081 tttgggaaga tggattctct gaaaaatata aaggttcact ttatgagtgg ataaaaaaag
    55141 cagcaaatac ttcgacttct ttaatttacc aacattagtg aaaatcgatt catctggaga
    55201 tttgtaattt agagtccttg tcttgattag aggtgtataa agctttttat tagacatttt
    55261 taaattccat tacaatttgc ttataaacta ggtttttaaa ggtgactatg gtaagataat
    55321 agagatatta ttttgtgatt aggagtgtat atatgaataa aaatggagaa tggagtgctg
    55381 cacgacggat attcattata ttaatggatg ttattgtata caatgcagca atatatagta
    55441 gtttcttact gaagtttcgt ggagagatcc cattacgaaa tttagaaact ttccaacact
    55501 cagcaattat tatttcagtg atttttattg cgttgaacat tttgttgggt gcatatgttt
    55561 tttataatcg aatgatcagt gacatcgtgt ttgtcacagt catcggtcag gtattgatga
    55621 cactagggat catggtagtg acttttgcgg gccgctggtt tgcttttcca cgtcttgtta
    55681 tcttactttc atttgttctc ggtacgcttt tcttaagtat ctatcgtatt ctcgtatata
    55741 aactttattt acgcgtaagt cgagataaaa aggtcgtcat tattgggtta gaaaaggatg
    55801 tcgcaccagc aatccaaaat tttggttcaa agaaaaacaa caaacataaa gtcgaagcag
    55861 tcattcttga taactattat gcaaacatca aaaatatgat taaagacatc gatattgtct
    55921 atcttgcttc tcatatcgaa gaaactgaaa aaatcaaaat ctatcaatta gttactaaaa
    55981 atgaaaaaaa attgtttttg aatacaacat ttgaaaactt gacattgatc aatccaaata
    56041 ttatgaattt cgaagatgaa agtattattg aagcatctgg atttagaatc caacctgaat
    56101 atgaactatt caaacgtttg tttgatatca tcgtttcact tgttttatta gttattgctt
    56161 caccatttat gttattcaca gcaattttag tcaaagtgac atcaccggga ccaatcatct
    56221 ataaacaagt gcggatcaca aaaaatcaaa aggaattttc aatctataaa ttccgctcga
    56281 tgaccgccac agccgaagca aaatctggac cagttttagc taagagcaat gatgcacgag
    56341 taacgcctgt aggtaaattt attcgagctg tccgctttga tgaattacca caaatcttca
    56401 atgtgctgaa aggcgatatg tcgatcgtag gaccacgtcc tgaacgttct ttcttcgtcg
    56461 atcagttcaa tgcagaaaac ccttattact acttacggca taatgttcgt gctggtatca
    56521 ctgggtatgc acaagtctat ggaaaatatg tctctgatta caacagtaaa ttacgttttg
    56581 atttacttta tatcaaaaaa tattcattga tgcttgattt aaagatcttg ctgcaaacca
    56641 tcaagatcct atttgataaa atgtcctctc aaggacttga agaagacgaa aatgtgatct
    56701 cattggcaga aattgctttt ccgaatgaaa aaatctttcg ctagttggga ggcgtaaaaa
    56761 tgaacaatcc gaaattcagc ataatcgtac cagtatacaa tgcagaagca actattggtc
    56821 gaacgattga tattctaaaa gaattggatt atgaagagta cgaagtcgtt ttagtaaatg
    56881 atggatcaac agatcaaaca caggaaatca ttcaagaact gattgaaaat gatgaacgtt
    56941 ttgaattgat tactacgacc aatcaagggc ccggattagc aagaaacgaa ggaataagac
    57001 agtcaaaagg agaatatctc ttattcttcg atgcagatga cacgccagta aaaacgatct
    57061 tgtcagatta tgatcatttg ctagaaaaga gtccaaaagc ggatcttgtt gttagttcgt
    57121 ttgtttttcg tacaatggac gagggaagaa tcgtttccga aaaagaaaac ttagttacag
    57181 aatgtcacta tctaactcac gaagagttta taaaagacat gtatcagtta atgaatgaac
    57241 agttgatgta tgttgtatgg aacaa
//
LOCUS       DACDMM010000004        47951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDMM010000004 DACDMM010000000
VERSION     DACDMM010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.004"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(38..1189)
                     /locus_tag="IWD16_000164"
     CDS             complement(38..1189)
                     /locus_tag="IWD16_000164"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9087252.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="IWD16_000165"
     CDS             complement(1202..1639)
                     /locus_tag="IWD16_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9087253.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="IWD16_000166"
     CDS             complement(1654..3420)
                     /locus_tag="IWD16_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP9087254.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="IWD16_000167"
     CDS             3684..4361
                     /locus_tag="IWD16_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9087255.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="IWD16_000168"
     CDS             4364..5491
                     /locus_tag="IWD16_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9087256.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="IWD16_000169"
     CDS             complement(5691..6131)
                     /locus_tag="IWD16_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9087257.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="IWD16_000170"
     CDS             complement(6142..8175)
                     /locus_tag="IWD16_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9087258.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="IWD16_000171"
     CDS             complement(8177..9880)
                     /locus_tag="IWD16_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9087259.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IWD16_000172"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="IWD16_000172"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP9087260.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="IWD16_000173"
     CDS             complement(10963..11988)
                     /locus_tag="IWD16_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9087261.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="IWD16_000174"
     CDS             complement(12197..13066)
                     /locus_tag="IWD16_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP9087262.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="IWD16_000175"
     CDS             complement(13525..15417)
                     /locus_tag="IWD16_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9087263.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="IWD16_000176"
     CDS             complement(15414..17141)
                     /locus_tag="IWD16_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9087264.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="IWD16_000177"
     CDS             complement(17157..17807)
                     /locus_tag="IWD16_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9087265.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="IWD16_000178"
     CDS             18011..18307
                     /locus_tag="IWD16_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9087266.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="IWD16_000179"
     CDS             18479..19099
                     /locus_tag="IWD16_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP9087267.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="IWD16_000180"
     CDS             complement(19191..20357)
                     /locus_tag="IWD16_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9087268.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="IWD16_000181"
     CDS             complement(20369..20722)
                     /locus_tag="IWD16_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9087269.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="IWD16_000182"
     CDS             20850..21872
                     /locus_tag="IWD16_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP9087270.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(22311..22745)
                     /locus_tag="IWD16_000183"
     CDS             complement(22311..22745)
                     /locus_tag="IWD16_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087271.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="IWD16_000184"
     CDS             complement(22855..24714)
                     /locus_tag="IWD16_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9087272.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="IWD16_000185"
     CDS             complement(24832..25992)
                     /locus_tag="IWD16_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP9087273.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="IWD16_000186"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="IWD16_000186"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP9087274.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="IWD16_000187"
     CDS             complement(27739..28437)
                     /locus_tag="IWD16_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9087275.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="IWD16_000188"
     CDS             complement(28394..30055)
                     /locus_tag="IWD16_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP9087276.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="IWD16_000189"
     CDS             30273..31673
                     /locus_tag="IWD16_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP9087277.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="IWD16_000190"
     CDS             complement(31925..33004)
                     /locus_tag="IWD16_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9087278.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="IWD16_000191"
     CDS             complement(33090..34049)
                     /locus_tag="IWD16_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP9087279.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35584)
                     /locus_tag="IWD16_000192"
     CDS             complement(34061..35584)
                     /locus_tag="IWD16_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9087280.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="IWD16_000193"
     CDS             complement(35594..36157)
                     /locus_tag="IWD16_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9087281.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="IWD16_000194"
     CDS             complement(36163..37026)
                     /locus_tag="IWD16_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9087282.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="IWD16_000195"
     CDS             complement(37040..37936)
                     /locus_tag="IWD16_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9087283.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39382)
                     /locus_tag="IWD16_000196"
     CDS             complement(38111..39382)
                     /locus_tag="IWD16_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9087284.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="IWD16_000197"
     CDS             complement(39372..40889)
                     /locus_tag="IWD16_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9087285.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="IWD16_000198"
     CDS             41087..41977
                     /locus_tag="IWD16_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP9087286.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="IWD16_000199"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="IWD16_000199"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP9087287.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="IWD16_000200"
     CDS             complement(42886..44358)
                     /locus_tag="IWD16_000200"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP9087288.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44995..45357)
                     /locus_tag="IWD16_000201"
     CDS             complement(44995..45357)
                     /locus_tag="IWD16_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9087289.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45580..46332
                     /locus_tag="IWD16_000202"
     CDS             45580..46332
                     /locus_tag="IWD16_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9087290.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46472..47395)
                     /locus_tag="IWD16_000203"
     CDS             complement(46472..47395)
                     /locus_tag="IWD16_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9087291.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47410..47682)
                     /locus_tag="IWD16_000204"
     CDS             complement(47410..47682)
                     /locus_tag="IWD16_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087292.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13979 a         9953 c         8268 g        15751 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttttt
    44941 attgtataca ttagatatgg agcaggttac cataaagatg attattagtc gttattaaat
    45001 agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac gtttgaattg
    45061 tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat cttctcgtgt
    45121 catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt
    45181 attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt gtgttcgatc
    45241 taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt ccactacttt
    45301 aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta gcttcatctc
    45361 attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac catctttcct
    45421 agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac cttttatcac
    45481 ttaatagtat aacataattt catgagaaaa aaatacaatt ttttcttgac catctcgtta
    45541 cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac aataaagaaa
    45601 atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga gattggactg
    45661 ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa agaagtcgat
    45721 cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga tatccaagag
    45781 gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca gcaaatgttg
    45841 ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac acttgatatg
    45901 tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag
    45961 ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag
    46021 gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga agaaatgcaa
    46081 actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc agaagatgaa
    46141 agcttagcag accgtatctt ccaagctcat aagaagtggt tatcttacag ctggcctgaa
    46201 tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga acggttcact
    46261 gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga aattatccaa
    46321 aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg acctaataac
    46381 aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc tccattcttt
    46441 cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta taaggtattt
    46501 tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg caagcttcca
    46561 gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa
    46621 tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca accatatccg
    46681 aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct tgttttcctt
    46741 tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga aaaagttgat
    46801 gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc cccaacgtga
    46861 tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta attgattgtt
    46921 ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa aaaactagag
    46981 gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac gtaatgatca
    47041 atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt tctttctgat
    47101 agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca atgatcccac
    47161 tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca atgcttttcg
    47221 tttttccata acttaactgt ctggcatttg cattagggat ataatcgact tctttcatga
    47281 ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg attacacgtg
    47341 aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt gccactgctg
    47401 cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg gaccgcattc
    47461 aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc cccaatatat
    47521 atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat tcctattcaa
    47581 cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg cataaaaata
    47641 attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc ctccttttga
    47701 tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt gccaaagaaa
    47761 agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat acattataat
    47821 gatactgtaa atatatcttt attattagtg aatggatatt tacttttaat agataaaata
    47881 ttataactat taaaaataac tattttcatt ataattacac acaaaaaaga gcgtgagaca
    47941 aaagtaaaga a
//
LOCUS       DACDMM010000005        41016 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDMM010000005 DACDMM010000000
VERSION     DACDMM010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.005"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            269..537
                     /locus_tag="IWD16_000205"
                     /pseudo
     CDS             269..537
                     /locus_tag="IWD16_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            1209..4610
                     /locus_tag="IWD16_000206"
     CDS             1209..4610
                     /locus_tag="IWD16_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9087293.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIE
                     RSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADG
                     KLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIR
                     GNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(5034..5204)
                     /locus_tag="IWD16_000207"
     CDS             complement(5034..5204)
                     /locus_tag="IWD16_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9087294.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(5398..5577)
                     /locus_tag="IWD16_000208"
     CDS             complement(5398..5577)
                     /locus_tag="IWD16_000208"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087295.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            5964..7076
                     /locus_tag="IWD16_000209"
     CDS             5964..7076
                     /locus_tag="IWD16_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9087296.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(7154..7447)
                     /locus_tag="IWD16_000210"
     CDS             complement(7154..7447)
                     /locus_tag="IWD16_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP9087297.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            7615..7929
                     /locus_tag="IWD16_000211"
     CDS             7615..7929
                     /locus_tag="IWD16_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP9087298.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            7950..9929
                     /locus_tag="IWD16_000212"
     CDS             7950..9929
                     /locus_tag="IWD16_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9087299.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            9942..10268
                     /locus_tag="IWD16_000213"
     CDS             9942..10268
                     /locus_tag="IWD16_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP9087300.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            10284..10802
                     /locus_tag="IWD16_000214"
     CDS             10284..10802
                     /locus_tag="IWD16_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087301.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            10802..12154
                     /gene="celB"
                     /locus_tag="IWD16_000215"
     CDS             10802..12154
                     /gene="celB"
                     /locus_tag="IWD16_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9087302.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(12238..13743)
                     /locus_tag="IWD16_000216"
     CDS             complement(12238..13743)
                     /locus_tag="IWD16_000216"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP9087303.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(13835..14425)
                     /locus_tag="IWD16_000217"
     CDS             complement(13835..14425)
                     /locus_tag="IWD16_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9087304.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            14604..16250
                     /locus_tag="IWD16_000218"
     CDS             14604..16250
                     /locus_tag="IWD16_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9087305.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            16299..17018
                     /locus_tag="IWD16_000219"
     CDS             16299..17018
                     /locus_tag="IWD16_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9087306.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            17497..18444
                     /locus_tag="IWD16_000220"
     CDS             17497..18444
                     /locus_tag="IWD16_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9087307.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            18456..19376
                     /locus_tag="IWD16_000221"
     CDS             18456..19376
                     /locus_tag="IWD16_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9087308.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            19417..20874
                     /locus_tag="IWD16_000222"
     CDS             19417..20874
                     /locus_tag="IWD16_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9087309.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            21219..21833
                     /locus_tag="IWD16_000223"
     CDS             21219..21833
                     /locus_tag="IWD16_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9087310.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            21826..23550
                     /locus_tag="IWD16_000224"
     CDS             21826..23550
                     /locus_tag="IWD16_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9087311.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            23547..24998
                     /locus_tag="IWD16_000225"
     CDS             23547..24998
                     /locus_tag="IWD16_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9087312.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(25471..27624)
                     /locus_tag="IWD16_000226"
     CDS             complement(25471..27624)
                     /locus_tag="IWD16_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9087313.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(27626..28672)
                     /locus_tag="IWD16_000227"
     CDS             complement(27626..28672)
                     /locus_tag="IWD16_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9087314.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            28822..30111
                     /locus_tag="IWD16_000228"
     CDS             28822..30111
                     /locus_tag="IWD16_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP9087315.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            30124..32826
                     /locus_tag="IWD16_000229"
     CDS             30124..32826
                     /locus_tag="IWD16_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9087316.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            32811..33668
                     /locus_tag="IWD16_000230"
     CDS             32811..33668
                     /locus_tag="IWD16_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9087317.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(33739..34596)
                     /locus_tag="IWD16_000231"
     CDS             complement(33739..34596)
                     /locus_tag="IWD16_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9087318.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            34807..36978
                     /locus_tag="IWD16_000232"
     CDS             34807..36978
                     /locus_tag="IWD16_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9087319.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            37046..37387
                     /locus_tag="IWD16_000233"
     CDS             37046..37387
                     /locus_tag="IWD16_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9087320.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(37663..39417)
                     /locus_tag="IWD16_000234"
     CDS             complement(37663..39417)
                     /locus_tag="IWD16_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9087321.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(39428..40264)
                     /locus_tag="IWD16_000235"
     CDS             complement(39428..40264)
                     /locus_tag="IWD16_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9087322.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(40266..40949)
                     /locus_tag="IWD16_000236"
     CDS             complement(40266..40949)
                     /locus_tag="IWD16_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP9087323.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        13305 a         7156 c         8495 g        12060 t
ORIGIN      
        1 gttttataaa agtgttattt tttggaaaaa ttcacaaata tgtaacgaat gtttttctgg
       61 aatttgctgt gttaaaaagc agtatgacta atataaatac tattgtcatt tatgcaattt
      121 tttatggata gtaattttag aggggaatca atgaaggaag aatagaaggg aaaaatagtt
      181 gcaaatacgc cagttagcaa aaatagcaat acatcttcaa gctttaaaat tcgagagaat
      241 catcttggta gtggaatcaa ctacaacaaa aggattaaac caattaatag atagaattac
      301 caatggaaaa atagaaaagg tagttattct atacaaaagt tgtttaattc gcttcggtta
      361 tgaacttagt gaaacccttt gcaatatatg gtacagaata tagaaattat tgacattaca
      421 gaaaatgatg aagatttaat acagattgtc actatttgta gatacctttt acaaggaaac
      481 cgagccaata aagctaagaa gatgattaag gagttgatgg aagacgatac aagttaagaa
      541 agtgaaacta cgcccaaata aagagcaaaa agagtaattg gacgtggaaa atgcaagaaa
      601 tgaaccatcg attcggtgga cagtttatta gtaatgacgg tttgagaaca tccatgacga
      661 gaatgaagga acatcctaaa tgcgtttagt tgaaccaagt gccaaataat atggccaaat
      721 aagcgatcag ttatcgatga atcccaagta ttctaaacta tatattcatt ttgatgctaa
      781 gcattgatat atttttttgg gattgatgaa ggttaaccaa tacagggatt cactgatgaa
      841 attctagagt ctgaccttgg tatcaacgat ttagtgattg tttctaatgg aaaagtataa
      901 aagtatataa gtacattaac aaaataacta ttagggttac gctccagctt gtgcaaggca
      961 ctggcaggag gaatcgtgaa atcataatta tggttaagcc acacggacaa ctaaaagtca
     1021 tacaaagcta tcgaatcaat caacagaaga caatcggcaa tcattagctt tgtttacagc
     1081 ttacattgca acgcttgagt tgaaccgaaa atacgaggga ggtaacgcag ccattttgag
     1141 ttggggaatc tttttagaaa attatcgggt accttgttgt ttagtcaaaa ttataagggg
     1201 gagtaatagt gaggaacaaa tttcataaac tgttaagtat gtttctagtt atgcttattg
     1261 tgatcaatca atttgggaac ataacggttc tagctgagtc tttagcagaa cagccagagc
     1321 gttcgctgac gatcgaagtt gatcaagagg aggctgcttt gggtgagcag ttccagttgc
     1381 gagtcagagg aacaccagac ctgctaaatg cattggtcat tgataagagt gaggatttga
     1441 ttgaagggga agtgaagcaa ctcaatgatt acgaacgtct gattacttat tcttcccagc
     1501 attcaggtaa atacgaattg accgcaagaa gcggtgatat tcaatcaaat acggtctcaa
     1561 tagaaatcag tgtaccagaa gaaagcacaa gtgagtcttt taatgtacca gaagaaagca
     1621 caagtgaatc ttttaatata ccagaagaaa gcttaagtga atcttttaat gaagccagat
     1681 catctgagcc atctataacg caggaatctc agattgaaag acaagaggag ctcttgaatt
     1741 atgacatcgg catctctgct gagcgcccca ctttgccgag caggcataca caaacgttaa
     1801 agggatacat tcaaggaggc gctggcgata ttcttgaaga atatggggag atggtcgtac
     1861 gagttcctag agcatcgatt ggcttaattg agttcattag agtggaggtc gctgatgatt
     1921 cacctttaaa gccactggca ttttaccaag aaggcacaga cgttgttttt ccatttacct
     1981 ttgattatga aggattaaat gaagccgctc agtcgtatac attcagtatc acgtttgaag
     2041 ttgctttctt tcaatcaggc ggtactgagc caaataatga ctttcaagtg agtcttactt
     2101 atgctgagcg agcagcaaac acggaatttt caattacgcc tattccttca tcaagaccca
     2161 ttttttcgaa attatactat ggctctagag aaatcgacag ccaaggaaat cttgggaatc
     2221 tgctgattga cgggcaagac gtgaccaaca gtaataaatt tattttgcag gtaaattatg
     2281 aaggatcacg aaaagacggt gtagtggtta ccgatgtact accgcaagga acaactttgg
     2341 caccgaatcc gactacatgg agttccactg gaaatagaga acctatttca ggtggggttc
     2401 ggatcgcaga agtaacgaat ccaggtgaag caaacgttag aactcattat gtttccgagg
     2461 aatttgtgga caaagtatct tttaataagg aaacgaatac acttgttgtg gactttggta
     2521 atctagcaga gggtcaatcg tattttattg aatatgctct tgaagtgacc gatgtcgact
     2581 tgttcaatga aggaagacaa gaggatgggt tttctcggaa tcgagcgagt atgacctaca
     2641 ataatcaggt aatgagccga gactaccgct tgagaatccc tgaaagtaat cttccggcaa
     2701 tctctttaag aaaaagtgtg gacaaaaatg ttatcaatct caatgaaaat gaactaacgt
     2761 atactctgca tgtctcgatg ttttctggaa caagaaatac gcttgttatt gaagatgttt
     2821 tggaagaagg catggaattt gtgtcatttc tatcagatat tccctctttc attgatgccg
     2881 aagtcaataa tggaatagtt cgtttcagtg cgaataatac gattggtggt ccgacaagtt
     2941 ttaccctctc ttttaaggtc aatgtagagg cgtatgcggt cggcgatcaa attttaaact
     3001 ttgcaacgct tgtgaatagt gacaatcgat tgaatacgag tactgtaaga acaaggaaaa
     3061 tcgatgggcg tattaaagta attaaaactg atgagaatgg ccaaaggctg gcaggagcaa
     3121 aatttgaaat tttatcaagc gattcacaag tgatacaaga aggtataacg gcagaaaacg
     3181 gagaattttt aagtgaaccg cttcctgtag gtagctatca agtccgagaa acagaagcac
     3241 cggaagggta tcttttagat gcgacggtac atgatgtttt tataaatgaa tactccatag
     3301 aagaagtgac gttaaccatt gaaaacatcc ttgatacggg tggtgttgaa ctaattaaac
     3361 atgctgaagg cagtggtgaa gtccttcaag gagctgtttt tgagttacag aaccgagaag
     3421 gagaaacgct acaaaccggt ttaactacag gagaagatgg aaaactggca attgacggtt
     3481 tagcaccagg agcatatcag ctggtagaaa ctcaagcgcc aatagggtat gaattagatg
     3541 cgacaccaat cgagtttgag atcgaaagaa gtcagacagc ggtggtagaa ttgacgaaag
     3601 aaaaccgact tacaccaggc ggtgtcgtgt tgacaaagat cgatgaccaa agcggggaaa
     3661 tcctgcaagg agctgttttt gagttacaga accgagaagg agaaacgcta caaaccggtc
     3721 taactacagg agaagatgga aaactggcaa ttgacggttt agcaccagga gcatatcagc
     3781 tggtagaaac tcaagcgcca acggggtatg aattagatgc gacaccgatc gagtttgaga
     3841 tcgaaagaag tcagacagcg gtggtagaat tgacgaaaga aaaccgactt acaccaggcg
     3901 gtgtcgtgtt gacaaagatc gatgaccaaa gcggggaagt cctgcaagga gctgtttttg
     3961 agttacaaga tgccaatgga accgttctgc agtcaggtct aaccacagga gcagatggaa
     4021 aactggcgat tgacggctta gcaccaggag tgtatcagct ggtagaaacc caagcgccaa
     4081 cggggtatga attggatgcg acaccaatcg agtttgagat cgaaagaagt cagacagcgg
     4141 tggtagaatt gacgaaagaa aatcgactta caccaggcgg tgtcgtgttg acaaagatcg
     4201 atgaccaaag cggagaagtc ctgcaaggag ctgtttttga gttacaagat gccaatggaa
     4261 ccgttctgca gtcaggtcta accacaggag cagatggaaa actggcgata gatggcttag
     4321 caccaggagc atatcagctg gtagaaactc aagcgccaac ggggtatgaa ttggatgcga
     4381 caccagtaac tttcaagatc gaaaaagaac aagaagtggc gatcttttta acaaaagaga
     4441 atagaaaagt tgctgatagc tcagatataa gaggaaacaa tccaacaagc agtggaaata
     4501 agcatcttcc aaaaactgga gaaaccatca ttagccagtt tatattgtca atattgggtg
     4561 tgctacttgt tttcatcagt ataaaattta gaaagaaacg aaatatatga gctaaaataa
     4621 aaaattgaat aagcatagtt tttaaaatca actagtaccc atcaattgaa tggaaactac
     4681 cgattttaga aggggtatac aaacaaatat tagtatcgga gtagagaagg ttgaacccaa
     4741 ccagtaaata agtaaagaca attttgtgct gcgttgagaa gtgaaaaata aacctttgta
     4801 gtaatactag ttaataaaaa atatacaaaa acactgcgaa aatcattctt gagtttcgca
     4861 gtgttttttg cctaatgact caagtttatt gggtaatact ttgattttct ttctacaaat
     4921 aatcagcgat cagttgtaat tttttctcta gttttaatgg ctctttgtca ataaggagaa
     4981 aaacaagtcg ggtcttttgt ctcggaaagc ttcaaccaac tcgtgaataa aggttacgcc
     5041 tccttgaaag gcgtggaaaa accaagtaat cgatcaatca ccaatttcgc atttggtaga
     5101 acatgtttgg tgagctgaaa gtaggtggcg ttcatgtctg taactaagaa ttctatttct
     5161 ttaggattag tgcagctaag gaaatagttt gttaatcgag gaattttttg tgtaggtaaa
     5221 ttttctcgat taatttttct gtctcgccat ccgcggaaat aaaactcagt ttatcctcaa
     5281 cggaaacgtg agaacgaaat tcacaaccct caacactcaa gacagaattc ttttggaaga
     5341 ctttggtaaa tagcttctca actctttcaa tgtgcgaata acggttgtca aagataccta
     5401 acaacttttc gaaataaaaa ataacgacac tttttcagtc agtaaagaag tgattttatg
     5461 tctgacatga tttgaaatcg agtgccttgg acggataaaa taactttgag cgttccaatg
     5521 gcttcgacag tttttacagg tatagcgctg cttttttagg cgtataacca ttggcatatg
     5581 attatattat tcaaagtgga caatggtttc cttcttcttg tttttcacca ccattaattt
     5641 cctatttcca tctaccacag cagaaccaca atttctacag acacatggag cagggaagag
     5701 aactgcagca acgatcaatg tttttttctt ctgtaaggtt tcgtaagaga cctctgtaat
     5761 catcaaattt ttgtctattg atctcagaat ttttttgata gaaccattca taaagcatat
     5821 cgttctctca gttgtttatt ttgtggtgat ttaatcatac aagaaaacga tatgtttttt
     5881 tataaaaaca tgaataggaa aaatagttga caagaaaatg acaacgccat atgatgttcc
     5941 atagtattta caacctgtca tagatgactg taaaaaataa aagtaaaaag aaaggacgac
     6001 aacaggtgga tttttatcag tctctacaac tagatccgtt tattttaaaa caaaaaatac
     6061 gagaagcagc ttctaaaaaa gaaaagtgca tgtatatttg ttcattgttt ttacgatcat
     6121 tattcatcgt attgtttgcc atttgtttta tcattataat tacaacatta tttgaatcga
     6181 agcataaacc atacgctgtg gttttattct gtatgttgat gagtattcgt tttgttgact
     6241 ttggttataa gatttcccat tcgatcatag gtttagcaat cgtcatgttc tcactattag
     6301 tagcaccgta tgtccagctg atcaaatggt cagttatggg catgttgatt cattttattt
     6361 tactaagcag catattaatg gcaaccgcta gtgatcccaa aatggggaat gcttctttgt
     6421 acggcttttc atatctcttt attgtttatt cgttacctaa agattcgttg aacaagaatt
     6481 tctttacaca aacaagtagc ttattatttt tgtttttttg ttggttttca gttctccttt
     6541 acagaaaaca tcgagaaaag aacaaagaca aaagcttgtt taaagagatt tttttgaaag
     6601 gcatgtattc gcaggaaaaa atttggatgc tcatctatgc atttggcatt agtcttttga
     6661 tcgttgcagg agagtatgtc ccttttcagc gattgatgtg ggcagggttt gcattttctt
     6721 ctattgtatc tagctatgga ttgatgtcga ttggtttcaa agaacgagca gtagaccgaa
     6781 tcattggctc attgattgga tgtgttttat ttattggtat cagccaattt attcctttta
     6841 actgggtcgg tatattaggt ggtctagctt taggtgtttg ttctacctat agatataaaa
     6901 cagtttttaa ttcttttggt gctttggcaa tcactgccag tttattcggg gttccaggag
     6961 cagtaacgat ccgcattttt gagaatatcc ttggggtatg tttaggaatc atgtatattg
     7021 gggtgactga aatacttatc cgaaaaataa gagaaaaaca cggtttgaat cattagatga
     7081 gatcgaataa aaaataagga aaaagtgaag atacacatgg atgataaatt gctatcattc
     7141 catgtgtatc tttttacggt aaatcagtga aacgttcgac tttcattttt aatttatact
     7201 ttttacggag ttcgtttatt tgagccatca tttcggattt gtgatgcttt tcgattgctt
     7261 cttcatcttt ccaacggtcg atcagtaaaa gagattctgg atcttctagt ggaaagtaat
     7321 agtcatattt ctcgtttcct tcttctgcac gaatcgcttt gacaatgttt ctttcgacca
     7381 tttcttctgc aaattttctg gcttgtccat ttgtacttgt atacaaaata ttcactgtga
     7441 tactcactaa atcccctcct gaaaaaattc tatcactagt atatcacggt gccagaaatt
     7501 attattcaag atatgataca cattttttcc gcaatgctac ggaaatatat attttgaata
     7561 gaaaacgctt acaagatatg cttggtttat aaaaataaaa ggagtgattg atttatgaaa
     7621 aaagcattga ttatttgtgc ggcaggaatg tcatcctcga tgatggcctc aaagacaacg
     7681 gatttcttta agcaaaaaag agaagaggta ctcgtagatg cagtttcagc tacagaaggc
     7741 gataatatga ttaagaatag tgattttgat ttatttctga tcagcccgca aacgacgatg
     7801 tttttggata aattcaaaaa attaggagaa tctgttggaa agcctgttgt tagtatccct
     7861 ttccaagcgt atgtcccaat ccctacaggc atccagaagt tagcagaggt gatcgaagag
     7921 aacatatgat ggaataggag tggatgataa tgaaacagaa ggaaaaattg ctgttgagat
     7981 atttgattga tcacaagaag gagtatgtca ccagtcaacg tttagcttct gaattgttat
     8041 tatctgatcg gacaatccgt aattacttgc agcggatcaa agagttagta gaaaaaaacg
     8101 gaggtaagat cattgctaaa ccaggttatg gctaccaact gcatatttta caaagactga
     8161 catttgactt gtttttgagt cggcaagaaa tagtacctgg atatgctaga gaaccgcaag
     8221 gattttatga gtccgaagat cgacaaaagt atatattgaa ccaattgctt ttggaagatg
     8281 ccattctttt tatggatgat ttggcagaag agctttatat cagtcgttcc agtttgacaa
     8341 aggacatgca agaaatcaag gagcgattga ttccatattc actaaaaatc gtttctaagc
     8401 atggtcaagg gacatggatc gaaggggaag aaagaaatcg acggcatttt atcatggata
     8461 ctttttttgg aaaccattat ggaaattcat tgaaagaata tttagggcaa agccgttttt
     8521 tcccagacat cagttttgaa gagctagtca ttatcattct cgatgaaaca agagaagcta
     8581 agttaaaagt ttcggatttt atcatacaga atctgacttt acacttggct ttggggatca
     8641 aacggctccg agaagggttt gaaatcaagg aattaggcat cgaacaagaa gtttctaagc
     8701 gagtagagta tcaagtggcg cagcggatcg ttcaacgtat tgaggtaatc gcaggtgtat
     8761 cgtttcccga agaagaaatt gcttatttga cattacatct catggctaag tcgaatcaca
     8821 aagaaaatca aaatgatcag gaactatcaa aggaattagc gaaagttctg aaaagattgt
     8881 ctcaggaatt tcatttttca ttagtagagg atctccagct tttcaatgga ctactcgatc
     8941 atttgtcacc gatgatgatt cgattgagcc gaggtattcg cattgagaac ccattgactg
     9001 aggagataaa gaaagaaaat cccaaagtct ttgacgcagt aaaaaggcat ttcagcaaca
     9061 tgccggcttt gaaaaactat accatcaatg aagatgaatg ggcctatttg gctttgcatc
     9121 tcatggcggc attggaaaaa gaaagagctg cgcataaact acatgcattg attatctgtg
     9181 caacgggtta cggcagtgcc caactattaa agaatcgcgt agtcagtgaa ttcggtaaaa
     9241 atattacagt ggtaagtgta aaaggctatt atgagatcaa tgaaacaact ttaaaaggaa
     9301 tcgacctgat tatttcttct attgatcttt cgacaatgtt tttcaaaatt ccagttttac
     9361 atgtcagtat ttttttgaat gaagatgatg ttcgtaaaat acgaaaggta gttggagaat
     9421 ctatacccta tcattcctca gatagaccga taccagcatt ttctctacag caaaaaaagc
     9481 agatttacac cgaacagttg tctgaaaaat ttttcaagac ttatacgtac gaaccgaaaa
     9541 aggaagaaat tcttcatgat ttgctgcaaa agctgagcat aaatgaagaa gaaggatata
     9601 taagagaaat gcgaaagcag atccagcaac gtgaaaatat gggacaaatc atattcagtg
     9661 atacagtggc agtcccacat cccgctgttc cagttggaat cgaaacaaag gtagccgttg
     9721 cattgattcc ttctgggatg cagtgggatc aatatcaaga aattcatttt gtttttttag
     9781 tttcgccttc ttatgtcgaa aatgaaggaa ttacggtcgt gactaaagca atcgttaggt
     9841 tagttgacca gctaaaagta cagcaggaaa tcttagctga acctattttt gagaatttca
     9901 gcagtaaatt tatcaaactg atcgaataag gaggaaaaca gatggctgaa aaaataaaca
     9961 gtgaaacact acaagtgact tcttttgata tcattttcca tagcggaaat gcacggacac
    10021 tgatacatga agcatttcgc tcgatgcgta aagagatgtt tgaagaagta gaagagaagt
    10081 tgcgacaagc aaatgaagag attcttcatg cgcatcagtc acaaactcaa cttctgaaag
    10141 aatatgccag cgggacaaaa atagaaatgg aaattatcat ggtccatgca caagatcatc
    10201 tgatgacgac gatgacatta ttggaagttg cacaggaaat gaattatttg tatcaaaaaa
    10261 ataaatagaa aggaaggatc attgtgcagg atacagtaaa aaggaaaatc gaattagaaa
    10321 aaaagcaact tagtttgaaa aacatgtatt tcaatcgtta tctaatcgta cgttacctga
    10381 ctgccttttt ctttttcatg aacatgcaat ggatgattct gcttctatca gctaaaagtt
    10441 taggaagtag tttaccgatg attctgattt tagctatttt gcctgcagta ggagaacaag
    10501 taaagcttta tcgaaaacac caaacgaacg ttccatggac aaagtggtat tttttgttcc
    10561 agagtgtctt taatttatta gtgattccag tgttgtttac ctcaggattt acattcttgt
    10621 atccttttat ggcaaataac aatggaggac agttatttgt tttcattctt gttgtaagcg
    10681 gtatttttgc cagtgcattt atacagtatc gtttaaagaa aatttcactc aaccaagacc
    10741 aacagtatat ccgtattaaa caatatgaga aagcgttata tttaggaaag gagaataact
    10801 aatgtttgat tttttgcaga aatacctgat gggacctatg gggaaaattg cgcaacttaa
    10861 aatcgttcgg gctgtcatgg cagcggggat ggcttctatt cctttcacga ttgtaggctc
    10921 gatgtttttg gttttaaata ttttgccatt accgttccca tttttagaag gattttttaa
    10981 tgcaactttt ttcaaagtta gtgacttgta catgatcgtc aatacaatga cgatggggat
    11041 tttatctatt tactttgcca ttgtgtttgc ttatgaatta acgtcaattg aacgagatga
    11101 acaaaaatta aatgttaatc cgctcacggg ggcattacta tctgtttttg cattctttat
    11161 gtgtatccct gaattgatca tgactgatgg taaaatcagc ttgattgctt ctatcaatga
    11221 gacagaaact gtagtaaatg gtattaggat gggctctttt gtcgaacgtt taggaacttc
    11281 aggaatcttt acagctatta ttatgtcgat tattgcggtc gaattgtatt gtatgtgtgt
    11341 caagagaaat tgggtcataa aaatgccaga tgttgttccg ccaggagttt ctcgttcttt
    11401 cactgctctg atcccaacat ttgtcattgc gtttgtggtg atgatcatca atggattatt
    11461 agttgcacta ggaacggata tcttcaaaat gattgctatc ccatttgggt ttgttacgca
    11521 gttgacgaat acatggcttg gtgtcatggt gatttatttc ttgatccatg cattgtggat
    11581 cgtagggatc catggagcga atatcatcac ttctttcttg accccgatcg tattagctaa
    11641 tatggcagct aacgcacaag gcgcaaatta tccgttggca ggagaattca acaattctta
    11701 tgtaaccgtt ggtggatcag gtgcgacttt aggattgatc atctttattg cattcctcgc
    11761 gaaatctgat cagctgaaag tgttaggaaa agcctcactt gtaccaggat tattcaatat
    11821 caatgaacca atcattttcg ggatgccaat cgtctacaat ccctatttag cggttccgtt
    11881 tttcttagct ccgatggctt ctgctacact cgcttatttt gccattcggc tggaaatcgt
    11941 caaaccgatg cttgcacaga tgccgtggcc ttcaccagtc gggatcggag catttgttgg
    12001 tagtggtggt gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca tcgcattcat
    12061 tatctggttc ccatttatca aattctatga taataaatta ttaaatgaag aaagagaaaa
    12121 ggcagcagaa ttagctagtg aaaagttaaa atgatttcta agaaaaaagg aactgtgaca
    12181 aaaaatcaca gtcccttttt ttcttagaaa tgggaaacca ctagtatata aataccatta
    12241 ttcttcaatc aaccgcttca cgattgcata atccccttca taaggttcat ctacaccatt
    12301 gattttttga tagggatcca cgccttttcc cgcaataacg actgaatctt gcggagcgct
    12361 ttctgctaaa gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa cgatcaaatc
    12421 tggattagta attgcttcat tgatttcttc cgcgattttt ttcggatctt caaatgctgg
    12481 gtcatctgcc gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc caaaatcatg
    12541 tcgacgggag attgctttgt ttcctgggct gcctaataca acaattacac ggccatctgg
    12601 atgttcattt ttggcaaatt tcagcaatgt tttcaagctt agataattat gtgcatagtc
    12661 cacatagaca tgcgctccat tggattggat caattgatcc attcggcctg gtacacgagc
    12721 ttcttttaat ccgtcttgtg catcttgact ggaggcacct gttagtgctg cggcaatgat
    12781 cgcactggct gcattttctt ggttgaatcc tccagccagc aaaatatcat aagcagtatc
    12841 gcagatagct aagcgatctt catgagaaga taaagtaaaa ccgtgagtac ctttatctga
    12901 acggatcact tgataattgc tttcttccga acgaccataa gtgatcgttg gaatctggaa
    12961 tagatcggct gtttccctaa gtaaatcaaa atagtcactt tcatgattta aaacaactgt
    13021 ttttgaattc aagatcaatt gtcgcttgca atagaaatag tcatcaaatg tcggatgttc
    13081 gatcggactg atatggtcag gcgagatatt caagaagatt ccgacatcta aagtcaagcc
    13141 gtatacgcgt tgtgttttgt aagcttgtga agaaacttcc atcactagat gagtcatacc
    13201 attttcaact gcttctgaca tcatacgata taaatctaat gactcaggcg ttgtcaaatg
    13261 ggatttgaaa tatgttttgc catctaacgt tgtattcata gtagaaagca atgctgtttt
    13321 tttatttgtt gtgtgatcca agattgcttt tgtaaagtac gcagcagttg ttttaccttt
    13381 tgttccagtg aatccgatca ctttcaattt attttgcgga tagtcgtaaa aagccatagc
    13441 aagaagcgcc attgctttac ggatatccgt tacgatgatt cctactgctg aattttcgta
    13501 tggttgttct gatacataat attgaatacc ctgttcgatt gctgaatcca aatattcttc
    13561 tttgaaattc attcctttac agaaaaacaa agtagaagca tcagcttttc ttgaatcata
    13621 acttaactga agaaattcag ctttttctgg tacatcaaga tgccagcctc ttgttgaaac
    13681 aaattctttt aatagatttt cttttaatag taaatgacgg atgtcttcta aagagaagct
    13741 cattgattga tccctcgctt tattcaattt cttggtttat tatagcacac tctttttaat
    13801 atatcgtttg tttccgctat gacaaaatcc agctttattc aaattttctt gacacagggg
    13861 acaagcggat gagtattttc ataaacaatg aactgactgt aatagatagg agaacgaata
    13921 gtgtctccaa caatccaaaa acataataat tcaaaaagac gataagacta ttggtaagta
    13981 aaagtccttg cggcattgga agccgaaaaa atttgtggaa aataatcggc ggtatcgtta
    14041 taccgccatt tgaggccccg actgtgtaca atgtgtaaac cccaagactg aacaaaagac
    14101 ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc cacttttgga acaatctcta
    14161 aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa aaatactgat tttcctaata
    14221 ggacagcggc tagaagcagc atcataagat tgatgctcca aacagtaact gagcgttgga
    14281 tagaaaaggc cgtttcaact aaatgtccga tggcgcccac tccggcggaa gctatatcgt
    14341 gagggagcaa gaaccaattc aatccaactg tgataaagaa aagacctaaa aatataacgg
    14401 gtagtttttt tatttcttgt gtcattgata tctcctataa atgttagcca taaaaggctg
    14461 tatatttctt tactttacca aaaaaacgaa accgaattca aaaaaacaat tgttcttata
    14521 aaagttatat aaaatcgatt gccagtttct gttttaccaa ttaaaatgat agtaatgttt
    14581 tgtagaaagt tggaatctga ccgatggata atcaaagaaa accagcatta gatcaattaa
    14641 caccgcaaga aaaaatggca cgaggctctg cttggatgac cgccagtaat atcatttcaa
    14701 gattgctagg cgctgtctat atcattccct ggtatgcttg gatgggcgaa aatgctaagg
    14761 ctgccaatgg attattcaat atgggataca atatatatgc attgtttcta ctgatctcta
    14821 cagcaggaat acctgctgcg attgcaaaac aaactgcacg ctataattca ttgaatgaat
    14881 atggcacttc acgacgatta tttatccgtg cattacagat gatgggcggg ttaggcctgt
    14941 tgtttgcttt gttcatgtac atcgcctctc cttggctagc tcatgcttct ggtggcggag
    15001 aagaactgat tccaacgatg cgttctttga gtatcgctgt attggttttt ccttgtatga
    15061 gtgtgatacg agggtatttg caaggcaatc aagaaatgat gccttatgcg ctgtcgcaga
    15121 ttgtagaaca ggtggcacgt attttttata tgctattggc tacattcatc atcatgaaag
    15181 tcatggatgg tgactatgtg acggcggtca cgcaatcaac tttcgcggca tttatcggta
    15241 tgctagcaag tattcttgtc cttttgtatt ttttgaagaa acatcaagcg tatacatcgg
    15301 cgtttattca ctacagcgaa aacaaaataa atattgcaac gaaggaattg ttgttagata
    15361 ccgttaaaga agcgattccg tttattattg tgggttcagg ggtaacgatc ttcaaattag
    15421 tcgatcagtt cacttttatg aagatcatgt ctgcatccac cgagtattcc aatgcacagt
    15481 tactggatct tttttcgatt tttagtgcca atccagataa gttgacgatg gtagttattg
    15541 cattggcaac atcgatcgcg tctactgggt taccgttgat tacagaagct gttacgttga
    15601 aagatcgagt aggtctggca aaattgacaa gcggaaattt acagttgttt tcatttttta
    15661 tgtttccggc gacatttggt gttatgttac ttgcctatcc gctaaataca ttattttata
    15721 caccggattc tctaggaagt aatgtactga tccaagcaag ttttgtagga ctcttcctag
    15781 gactatatat gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag gcggcaatta
    15841 attatttggt tattgggttt ctagtgaaac tccttcttca atacccagcc attcgtttat
    15901 ttgaagtata cggcccttta ttggcgacaa tgatcggatt tgctgtgtct tgtaccttga
    15961 tcttaaagag aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg cgcaggacat
    16021 tgctgatctt tattcttacc ttgattatgc ttttggcttc gtggatcaca aagatgattt
    16081 ttggtgcttt tttaaacgaa gatagcaaat tccagtctct attgttgatt cttgtagtag
    16141 caggtatagg ggctttagtg tatggttatt tagctttgaa aatccgcttg gcagaaagac
    16201 tactaggacc aggaatagcg cgtttacgac ataaattaaa aataaaataa gtgaatcaga
    16261 agagctagat tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat aagtttttag
    16321 cagatatggg acttggcagc cgcaaagaag tcaaaggatt aatcaaaaaa ggaacgatcg
    16381 aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt aaatgaattc gaagatgcgg
    16441 ttacttattt aggagaaatc attacgtatc aaaaagattt ttactacatt ttgcataaac
    16501 ctgcaggagt catttctgct actcaggata atcatgatca aaccgtaatc gatttattgg
    16561 aagaccagga ttatcgggaa gatctttttc cagtcggccg attagataaa gacacagagg
    16621 gcctcttgat tttaacgaat gacggggtgt ttgcccatca gttactatca cctaaaaaac
    16681 atgtggagaa agaatatcta gctgaggtta gcggtataat gacagaagaa gatatccgtt
    16741 tatttgcaga aggactgatt attgatggcg atgagcagac attgccggca aaactccaaa
    16801 ttgatcaagt agatcaagag acagaaactt caattatccg tctgattttg catgaaggaa
    16861 agtttcacca agttaagcga atggtcaaag cagtaggaaa agaagtctta tatttaaaaa
    16921 gaatccgtat gggaaaattt tggctgccta aagatttgaa taaagggcaa tatcgaccca
    16981 tgaccaaaga agagctagaa caagtgagag aaaaataatt gaatggatag ctaaaagaat
    17041 aagcagagta gttgtagcgc catctgaaaa gcagacttgt ccgtctgact ttcggatggc
    17101 ttttttgtga aatttcggct tatttccgta gaagctactt ctggaacacc gtttgttcgg
    17161 aataagggac tgtgacaaga cttttgttac agtccccatt gttccttgtg actcagagtt
    17221 agatagtaag gacacgatct ccctaatact caggccaaaa cgctgaattc acaacctctg
    17281 ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg
    17341 aactattttc ccgttttttt ctctaatact caggccaaaa cgctgatttc acaacctctc
    17401 ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt gaaagcgcta
    17461 taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat
    17521 cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt accgggtatg
    17581 ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc attcaaaaat
    17641 tttcatattt cagcagatgg gttcttggct agcttgaggg aaagtccttg ggtgggttgg
    17701 gaaaatttca aatttttatt ttcttcggat caagcatttc tcatcactaa aaatacaatt
    17761 ttatataatg tagcatttat ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt
    17821 atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc cttactacct
    17881 tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag tcctgacaaa
    17941 ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg gtataatgaa
    18001 ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat tggttataac
    18061 agcattattt actttgcatc tgttatggga atcgatccga cttattatga agctgctatg
    18121 gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca acttattcca
    18181 ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga ttttggcctg
    18241 ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt tttggatacg
    18301 tatatctaca atggcctgac atccacagga gattttggta tgacggctgc tgcaggactt
    18361 taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt
    18421 gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag
    18481 tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg attgcactct
    18541 ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt accgatgagt
    18601 ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata tttggttata
    18661 cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc actgtacttg
    18721 ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct tatgcgattt
    18781 cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt
    18841 tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag ttaaaagata
    18901 cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt gtgatgcgca
    18961 cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc gatggagcta
    19021 gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt attgcgacaa
    19081 tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta ctatacatcc
    19141 aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt aatattcaat
    19201 atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc tcagttccag
    19261 gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg atcagctatc
    19321 catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa
    19381 acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa gaaactagcc
    19441 tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg ttctggttct
    19501 aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat cggagataaa
    19561 ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa agagatcggt
    19621 gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat gaacgtcatc
    19681 atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc caatgcccaa
    19741 aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa agcttataat
    19801 gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata tgctttccca
    19861 gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt actagataaa
    19921 tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt cttgaaggaa
    19981 ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt caaagtccaa
    20041 gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct ggatggagat
    20101 gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt acgtacaatg
    20161 catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa taaggattat
    20221 ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta tgattatggc
    20281 gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat tactgttcca
    20341 ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac ttcaaaaaat
    20401 aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt attgaatggg
    20461 cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga tgataaaatc
    20521 aaattgctag atggttatca gccaaataca catatgtctg cttggaatac aggaaacaac
    20581 aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg tgatcaatcg
    20641 attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga cagtgtcaaa
    20701 acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt gaatacaggg
    20761 actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa ggctggctat
    20821 gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga ataataatga
    20881 ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg aataaacggt
    20941 gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg aaagccatat
    21001 tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc ttggtaaacg
    21061 gtgttcaatc agctaaccct cggtattcgc tcacaaaacg acaaaatatg aggaactatt
    21121 tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct ttttttgtat
    21181 aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag gttttggaag
    21241 agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg atatatacat
    21301 ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat gaactatttt
    21361 atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat atatataaaa
    21421 gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact gctttattga
    21481 gttacaattt atatttatct gttcaaatca aagggctcat ttttttagct attgatttta
    21541 ttttactttt tgccttgctc tgcctatttg ctacttatca atacagtatg attttagata
    21601 gtgaatatac gatctctgtt cctaatctgc taaagctaag ttttatttcg gttttttcca
    21661 gcttttcgtc ttttctaaaa ataattattg gtagcggtat aattttaggc gtgacttggc
    21721 aattcaaagg attgatcctg tttggtgtca ttggtctttt gacggtttgg aatggtacga
    21781 tgacaacaca ttggcgtgaa gaattagata aacagttgga aagttatgaa taagtttcgg
    21841 attctatttt caaaaggaac cttactaaat cgtctgttgc ggaaatacgc ggttatcatg
    21901 atcggtgtaa cgatgactgc taccgttatt tttagtatcc atacttggga acaaaatcaa
    21961 aaacaggcag aaaacatgac aagtgatgcc gttcaaagca caagcagaat gctgaatgat
    22021 aaaacaacct tgagtaggat catcaaaaac cagcttgtag ggaattccga aaagatcgaa
    22081 aatgtgacga cctacttaac aaaacccata gatcagtatc tgatgtatgt atacgaacag
    22141 caaaacagta ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat
    22201 tatgaagaac taagtgcgat atatatcgtg ttgaatcaac taccggagta ttatgaatct
    22261 tctagagaaa acaaaggtgg ggaaataaaa aaggggacac caaaattact agacgccttt
    22321 tatatcaagt tgcctatcac acaaggagga gctgagtcag gaagtatttt tattggtttt
    22381 caaaagaaag aactagatat gattttagaa aatctaacgt cattcaatgg actctcttta
    22441 tacatgattt ctggaacgac aaatcgacta tacacattcc atgataataa aatagctaaa
    22501 gaaacgtttg aaagacaaga aatgatcatc acagagagtc tgaagaaaaa ttcacaattg
    22561 ccgattgacg atctggaaaa aaacaatttt gttcaacacc aagaactgtc tgatgattat
    22621 cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc
    22681 ccgctgatca ttggtatcat tattttagac aatatactgc ttatctttct ttataaaaca
    22741 ttcaaacgat attcacagca agtatctatc gtcatgcagg ccatggatca aacagcagca
    22801 gggaatttag agacgcggat cgacataaca aacacggaat atgaactgag ggaactttca
    22861 atcggtatca acgaaatgtt ggatagcatc aatcagtttg tagaagacat ctacaaatta
    22921 gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag cacagatcaa tccacacttt
    22981 ttatataata cattagaata tatccgaatg tatgcaatca gcgaaggaag tgaagaatta
    23041 gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca agagaaaacg
    23101 atcactttaa aagaagaact ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt
    23161 tatcctaatc gagtagccta tcattttatg atcgatcctg atttggaaaa gatcgaagta
    23221 cctaaatttg tgatccagcc attagttgaa aattatttta aacacggtat tgattttact
    23281 cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc
    23341 atcaaagata atggaaaagg gatgactgaa aaacggctaa aacagattga agaaaaactg
    23401 tctcatccta aagtggaact tcacggttct atcggtctgc aaaacgtgaa tgaacgttta
    23461 cgtgccagct tcggcagcag ttattatatg tcgcttgaga ataatgagac aggtggactg
    23521 accgtttcta ttacattcaa ggagggatga gcgtgtatca agtgttattg gtagatgatg
    23581 aatacatgat tttgaatggg ttaaaaaaga taattgattg gcaaagttta ggctttcaaa
    23641 tagtagcaac agcagagaat gccaaagaag gactagctgt acttgaacag cgacagattg
    23701 atttagtagt aacggatgtg acaatgccag aaataaatgg actggaattt attgaagcgg
    23761 ctcaaaaaga acgtcataat tttgaattta tgattttatc aggataccaa gaattcgatt
    23821 atttgaaagg aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc gtgaataagt
    23881 tcgaattggt tgaaagcctc aagaaaatca aaacacggtt agatcagcaa aatgaacaaa
    23941 aaaatcaaca agaaatctat caagaactgt tattcagtca atggttgaat gaagaattgg
    24001 atgaaataag tgaagaagaa ataataggac aaataagtga gaagcagcat aggatagtat
    24061 tgattcaact ggcaagaact catgatactc ttatcaatag atggttgcaa gaacggcaac
    24121 aaatctttta ctatcaacga aattacggag accgtgtatt gatcacgttg ttgttggagg
    24181 cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat caggaatggc
    24241 tgatcagtat cggggaagag acagattctt tggacaaaat ccctgaaaca ttccaacaag
    24301 cgaaagacaa tctacagttg catcaatttt atggagataa agacgagcat gttttttacg
    24361 cagtacaagc agaaacaaaa gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc
    24421 aaaataaaca attggaagtc gctcaaaaaa tgatcactga tttttttgaa cagtttcagt
    24481 tagcagccat gatgccggaa gatatacgat attccagttt tttactgttt atggagattc
    24541 aacgggaatt gattgatctg gaagatgagg aatatttaca aggaatcgaa aaaatccatc
    24601 aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa aagcatcaga
    24661 gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac cagcattatc
    24721 aagaaccact tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc atgtatctag
    24781 gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac catttacgta
    24841 tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa catcaatgag atagcaagtg
    24901 agattggcta caacaacacc acgtatttct ctaaattatt caagaaaatc gtcggcagat
    24961 cccctaaaga gtaccgagaa aacagtggag aatcataaaa gaaaaagcca atgtaatgaa
    25021 ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta cttgaggcaa
    25081 ggcagctttt tcatgacctc tcgataaacg gagttcaaaa gccagtttct cggtattaag
    25141 ccaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttaa tactcgaaac
    25201 taaacagctt tttcacgacc tctccactaa acggtgttca atcagctgac cctcggtatt
    25261 agctcaaaaa atggcaaaat atgaggaact attttgccat ttgtctctct aatactcagg
    25321 tcagaacgct gatttcacaa cctctcaata aacggtgttc aaaagcttgc atctgcggta
    25381 ataagaccaa ccaaacgaaa aatatgagga tctatttttg cttggctgtt cttattactt
    25441 gatgcaggac agctttttca caacctcttg ttaattatag tttgataagg aaaaaggcaa
    25501 atcttttgct tgatatttct tagggtgagg gatgatccct aattggtaag agatcgtgcc
    25561 gcctttcagc aaatcttgat gggtaaaata taatttcgta tattctctgt ccatccgttt
    25621 gattttatgg ataaattgtt gctgcggccg attaggcgtt gcttcaatcg tcaattcctt
    25681 tccatttgac aaaagcaaaa cagccttgtc aattaatggc ataccgataa catattcacc
    25741 ggttccaggc gtgacaggat aaaatcccag actattgaag atataccaag cggccatact
    25801 tccattgtcc tcatctccag ggtaacccat tggagaatcg tcgaaggtct gagtcatcaa
    25861 ctgtttaagc agtggctgtg ccatttcagg ttgtcctata taactgaaca agtaaggata
    25921 gtgaaaactt ggctggtttg aaatagctaa ttgtccaaat tctatcgcgg ccatctcact
    25981 catttcgtgg atctcaaaac catatcctcc tgggtcaaac gttgggggct gattgcacag
    26041 ctcaatgagt ttttctttat atctctcact gccaccatat tttccgatca gtcctgaaaa
    26101 atcgtgataa acagcaaagc tgctttgcca agcacttcct tctgcataat cttgtcccca
    26161 acgattagct gaaaatggtt ctctgaactt gccatcttta tcttttgaac gcataaatcc
    26221 tgactcatag tcaaagatat tttgatagtt caatgattgc tgctgatatt tcttcgtcat
    26281 ttcctcttct tctagtacct tggcaacttg gctaatacaa aaatcgctat aagcatagtc
    26341 taatgtgtga ttgactgatt cgtggtagtg attaggaaca tagccatatg tcaagtaatc
    26401 ttctgttcct tggcgtccat aatttggatg tttgctttga accgtagcac ctttttccat
    26461 tgccgttaga aattcaggca tcaagtctcg tcggatatct tttacagcag catctgcaat
    26521 gaccgcatcg atcaatgtcc caggcataag tccacgttca tcaggagaca gccattttgg
    26581 caaatagcca ctttctctat agctgttcaa aaaaccttcc agcatttctt cgtatttttc
    26641 tacagcaatc aaagaataaa gaggataaac cgttttatac gtatcccaaa acccattatt
    26701 tgtatagaga acaccaggtt ttatagatcg actagtcgta tcatagtgga ttttttgccc
    26761 actaggatca atttcataaa atgtttgtgg aaataaaaac acccgatata aattatgata
    26821 aaacgtccgc aattgttgtt gatcatggtg tgtgagctgt atacgatcaa aataggactg
    26881 ccattgttct tgactgtgag caaggtaagt ctctttgctt tggttttgtt ctagttccag
    26941 gttataagct gcttgttccg agctgataaa agatgtccca aatcggatag tttgtttttt
    27001 tgtctgttca aaatacaacg ggatcgagtc gctatttttg ttggaaacag ctaactcatc
    27061 aattgttaaa ggatattcaa atctcaatat aaaataaaaa gaaaaatcag gatcttctga
    27121 accagcaaaa tgtgtgattt ttccttccac tgtgtaagga tctataacaa agagctgatg
    27181 gcgtcccgga aaggataaaa gtaaaccgct gtcatttttg ctgtaatcaa tagataagat
    27241 tccgccatac atgctaggta tcaatgtaga acagatgcca taacgtaatt gacagatcga
    27301 cagatgagtc gggcaaaata tactttcttc tgggcggtaa gaactttgag catggaaaag
    27361 ggtgttctcc tgtagttttc cattgatcgg cgtaaacagc atatgactga aatcacccat
    27421 ccaaggactt ggttgatggg taagacggta gccttgaaaa acgcggtcat caggatgaaa
    27481 ccaccagctg ccgttttgat ccgtagtttg cggtgcaaaa taattcatcc caaaaggaac
    27541 gccagtataa ggctggcagt tcccattaga gaaatttggc tgattcgctg ttgcatgtct
    27601 agtatctatt tgaccgatat tcattttatt tctcctttgt tgattgtctt ttgatgaatg
    27661 aaactggaaa tttactatcg gatggtcgat cttttgtctc taaccaagtg accaaagcat
    27721 ctcccgcaag cttacctaac tgttcaaaat cttgtgcgat ggttgtcagt ggcgggtcaa
    27781 caagagctgc tgcttggata tcatcaaatc cgataattga aaaatcttca gggatatgat
    27841 aaccatgctg tctcaagaga ttcatggtct ggatagcgac aacatcgttt tcacaaacaa
    27901 aagcagttac ttcatactta gtgatataag aaagcaattc atctaatgtc gcgcttttgt
    27961 catccagtga agtgtggaaa gtcaaaccag ctttgtccaa tgcttcaagg taaccaaggt
    28021 aacgctgtcg aacggattgt ggatgagtct gcgtaccaaa caaataagcg atttttgaat
    28081 gtccctgttc gatcaaataa gaggcagcaa gcgcaccgcc ttggaaatta tcggaaagaa
    28141 cggtaggaaa aggtaactca tagatttttt tatctaatat gacgacaggg aattctttca
    28201 atgaaagctc aaaaagtaag tcaagatgtt gttctgtatc aagagcataa taaatcaatc
    28261 cgtcaaattc attgattagc tcttttgctg ttttattttg tagaaagtca agcgtcgtca
    28321 tcatgacatc aatctgttta tcttgcatca cagggttcaa tcccttgcta aaatcaccaa
    28381 cagacaaatc agcgacatat ggaagaagaa acagaatccg tcgtgttttt gtaggagaag
    28441 caagaatctg ttccggagaa gcggatttaa cgaaactgcc tttcccgcga acgcgataaa
    28501 taagtcctaa ttgctctaat tcagtcaatg cacgtttgga agtgattctg cttactttat
    28561 aggtacgtga aagttctttt tcagttggta gttggctgtc tacaggtaat tcaccagtta
    28621 aaatagccgt ttgcaaatca ttaaatattg tctggtacaa tggttgagtc atataaacac
    28681 ctcttaatga tatatcatat tcaaataata ccataatgga tgaaaaagta aataaaaaga
    28741 tattttttga aagcgtagac ataattggct gtatgaagta taatctttaa agatatatca
    28801 aatagaaaga aggttttatt agtggtttat aaagatatac caaaatcggt gcagaaattt
    28861 atggaagata tcacacaaaa atgtggggat atccatgcgg actgggcaaa gaattttaca
    28921 gcagcatttg ccaatactct gttgacaaca gtcaaaaagc aaaatgacgg aaccaccttt
    28981 ttattgacgg gagacattcc agcgatgtgg ttaagagact ctactgcaca agttcgtcct
    29041 tatttagtga ttgcaaaaga agatgaggaa ttagctgaca tgatcagcgg gttagtcaaa
    29101 aagcaatttt tttatatcaa tatcgatcca tatgcgaatg ccttcaacga agaagctaac
    29161 ggtgctggcc accaaacaga tcatacaaaa atgaacgatt ggatctggga aagaaaatat
    29221 gaaattgatt ctttatgcta cccaatacag ttggcctatc ttctttacaa aaatacagga
    29281 aaaactgatc aattcaatca atcttttatt gaaggtatca aaaaaatctt ggacgtgttc
    29341 aaaacagagc aagatcatga acactctcct tatacatttg tacgagacac gacaagaaaa
    29401 gaagatacgc tgatcaatga cgggcgaggt gcaaaaacag cttttacagg aatgacatgg
    29461 tctggtttcc gtcctagcga tgatgcttgc cgttatggat atctggtacc atccaatatg
    29521 tttgcggtgg tcgtgttagg ttatgtggaa gaaatttttc aaacgatact cgatgataaa
    29581 gaaattgtac agcaaacaca gaaattgaag aaagagatcc aagaggggat tcagaatcac
    29641 ggacgaacgg ttaatcaaaa agggcaaacg atttatgcat atgaagtaga tggaatggga
    29701 aacgccacag tcatggatga cagtaatgtg cccaatctga tcgccgcacc ttacttggga
    29761 tattgttctg aacaagagga agattatctg caaacaagaa gaacattgtt gagtaaagaa
    29821 aatccatatt tctatgaagg aaagtacgca aaaggaattg gatcctccca tacgccagaa
    29881 aaatacgttt ggccgattgc attagctatg gaaggaatga caacgtcaga caaatctgaa
    29941 aaagagcgga tattgaattt acttgtagca actgacgcag gaacacacct tatgcacgaa
    30001 ggattcgatg tgaacaatcc tgaaaattat acgagagaat ggttttcttg ggcaaatatg
    30061 atgttttgcg aattagtcat ggattacttt gatattagag tagaaaagta ggaggtatcg
    30121 taaatgaaaa agaaagtata tatcatttct catagccact gggatcgtga atggtacatg
    30181 gcttacgaac agcatcacat gcgactgatt gaattgatag acgatctact agagctattt
    30241 gaagttgatc cgtccttcaa cagttttcat ttggatgggc aaacgattat tttagatgac
    30301 tatcttcaag taaggccaga aaaaagacag gcaatccaac aagcaatcaa tgaaggaaaa
    30361 ttgagaattg gaccatttta tatcttgcaa gatgattttc ttatcagctc ggaatcgaac
    30421 gtgagaaata tgctgattgg catggaagaa agccgtaaat ggggaacccc tgtcatgtta
    30481 ggctattttc ccgatacatt tggaaatatg ggacagacgc ctcaattaat gaaacaagct
    30541 ggtatttcag cagcagcgtt tggccgtggt gtaaaaccaa ttggctttga taatcaagtt
    30601 ttggaagcag aaaactattc ttcccaatac tcagagatgt ggtggaaagg ccctgatcaa
    30661 acagcaatct ttggtctgct atttgccaat tggtatagca acgggaatga gattccagta
    30721 gaaaaagaag cagctcttgc cttttggaag caaaaattag cggatgcaga acaatatgcc
    30781 tctacgaacc acttattgat gatgaatggt gttgatcatc aaccggtaca aaaagatata
    30841 tcaaaagcaa tccatctcgc aaatgaactg tttccagatt acgagtttat tcattctaat
    30901 tttactgact atctggaagc agttcaaaaa gatgtgccag aagacttagg ctcagtagaa
    30961 ggagaattga caagtcaaga aacggatggc tggtatactt tagccaatac agcatctgct
    31021 cgggtttact taaaacaatg gaataccaag gtccagcgac agctagaaaa tattacagaa
    31081 ccacttgcga cgatggctta tgaagttagt ggaaactatc cccatgatca attagattat
    31141 gcttggaaga cattaatgca aaatcatcct cacgacagta tttgcggttg ttcagtcgat
    31201 tctgtccatc gggaaatgat acctcgtttt gaaaaagcag atgaagttgg aaagtatctt
    31261 gcacaagatt ccttagaaca gctgaccgca gcaatcgata cgaccggatt cccaaaagac
    31321 agctttccat ttgtcattgt aaatactgca ggaatggata aaactggtga agcagaaata
    31381 accatcgaat tagaaagaaa aagattcgct gaaggaatac ctgaacaact atatcaagag
    31441 ttagaaaatt tgcctaaacg taagtatcat gtagaaacaa aaagtggggc cactatacca
    31501 gctattttat ctgaagaaac tgtccaattt ggttatgatt tgccgaaaga ccgtttccgt
    31561 gtgccatata tggcaagaat gatcaaagtg actttaccat tagaaaacat gccagcattt
    31621 tcttgggaaa cattcgcgct tgtagaaggg gaagcgaaag cagaagaaaa ggaaacaatg
    31681 attcatcaga gtggacgaat catcgaaaac ggccctctac atttgacgat tgaaaaaaat
    31741 ggtaccatta caatggaaga tcgcaaaaac aaacgaaagt tgaataatct gcatattttt
    31801 gaagatatcg gcgatatagg aaatgagtat atctttaaac aacctttctg tgatcagcca
    31861 atcctatcca gtaacaaaga aaactctgaa gtaaaagtat tagtagatac accggaaata
    31921 gcaaaaataa gtattctaca ggaaatggaa atccctgttt ctgcagacga gcgtttagaa
    31981 aaagaacagc agatggttgt ggaatttcgt tatcgaaaag cagaacgatc caaagaaaaa
    32041 cgaatccttc aaataaaaac aatcatgaca atcagaaaag atagtaaaaa aatcgacttt
    32101 gaaacgactc tcgacaatca gatgaaagat catcgcttac gtgtcttgtt tcctacaaag
    32161 ttacatgtgg aacatcacga agcagatagt atttttgaag tggtgaagcg cccaaaccat
    32221 gtgtctaaaa gttgggaaaa ccccacgaat ccgcaacatc agcaagcatt tgtcaatatt
    32281 catgatgaag agtacggtgt aacagtcgga aactttggtt tgaatgaata tgaagtaaca
    32341 gaagacggac aaatcgcagt gacgctactt cgcagtgtgg gagaactagg cgattgggga
    32401 tattttccaa caccagaagc acagtgtcta ggtgagcatc ggttcaatta cagcattgaa
    32461 ctacatggac cagaagaaaa attctctact tatttgcatg catacgcagc acagattcct
    32521 ttttccactc aacaaatcaa acatcatgaa ggaacattga tttcaaaaca acaatattta
    32581 acgataaaaa gtgaaacgtt tgcaattaca gctttgaaac gcagcaagtt tagtgataaa
    32641 gtagtagtaa gaggtttcaa tatgtctagt catttagaaa aattggaaat aactaaagac
    32701 aatggaaaaa ctgtcatatt gaatctgctt gaagaaccaa cgaagcaagc ggttgtccca
    32761 ataatccaac cttatgagat tcggacaatc ggattcgagg aggaaaacga atgtatggcg
    32821 gaatagaagc aggcggaaca aaatttgtgt gtgcggtttc agatggtgga gaaattttag
    32881 aaaaattaag tatccctacc acaacaccag aagaaacatt ggatcaggta tttgaatttt
    32941 ttgatcgtta tgaattgaag ggaatcggga ttggctcatt cggcccgatt ggtatcgaca
    33001 aaacaaaaga taattacgga tacgttttgg caactcccaa aaaagggtgg gcacaattcg
    33061 attttcttgg cacaatcaag gaacgctaca atattcctgc tgcttggaca acggatgtca
    33121 atgctgctgc atatggggaa ttgaagcaag gagcagcaca aggaaaaaat agttgtattt
    33181 accttaccgt gggaacagga atcggtgcag gtgtcgtgat caatgaagaa attttttcag
    33241 ggatcgcaca tcctgaaatg ggacatattt gggtgaaacg ccatccagat gaccattatg
    33301 aaggtacttg tccttatcat aaagattgtt tagaaggatt agcagcagga ccgtctattg
    33361 aggcacgcac agggatcaaa gggcaagatc tgccgcaaga tcatcctgtt tgggctattc
    33421 aggcttatta tattgctcaa gcactgatca actatacgtt gacgttagca cctgaaaaga
    33481 ttattttagg cggtggggta atgaatcagg atcatctcct tcaaaaaatc cgtcagcaat
    33541 ttgttgaatt gatgggtggt tatatggaaa cacctcaagt aagtgaatat atcgttcgtt
    33601 ggggactgcc gaatgaaagt gggattatcg gtagtttgct gttagcagaa cacgaacatc
    33661 aaaagtaagc agacaaatta cacaaaaaga acagagtctt atcattatct gctgataaga
    33721 ctctgttctt ttttatttct attcccataa tccaggtaaa gctttaccga ctattttttc
    33781 ttgagtaaat ggatggatca tgtgcagttc taatgcatgg agcatcagtc gttcagctgt
    33841 tctattttgg tagagagggt ctccaacgat cggatgtccc ttatgagcta aatgaactct
    33901 gatctggtgt gtacgtccgg tttccaaaac acaatagact tgtgtttgtt cagaaagtac
    33961 tttgttgaca gtgacatgtg tcaaggcgct ctgaccattt ttatcatcta cgaccctttt
    34021 tctccgatca tggcgatctc gtccaatttt attggtgatg gtcatatctt tggcaatttc
    34081 tccccatacg atggcttgat atctccgata gatcattttt tgttccaaca ttcttccaag
    34141 tatcggcaag acaaatggat tttttgcaaa taaaacggca ccgcttgttt ctttgtccaa
    34201 gcggtggaca acgtaaggtc gttgctgttt agtgtcgaga taagcagcta aatgattgag
    34261 taaggtactg ttttctgtcg gttcatttgg atgagtcttc attcctgcag gcttatccac
    34321 tataatcaaa tgctcatctt catataaaac acggatgtgc ttcgcatctc ctaattgaac
    34381 ttcttgataa ttataatctg tttcttcgaa taacaatgtg atctggtcat tgccctttac
    34441 ttcatgatgg aacaaagcag gttgttgatt gatccaaacg tttttacgta tacgcaaaaa
    34501 gtgtcgaacc tttctaggaa ctaaccattc cttttcaagc aagtcgcgaa ttgttgtttc
    34561 aggatggttc ataggtaaag tgatcgtgat tttcacaagt gtactccttt cagtgcaatt
    34621 gcagactaat tgtaccattt ttaaaacata tttaagaaaa aacttacatt tttctgttca
    34681 ttcttatgtt actctacttg cactaacgta aaaaaacata ttgaactaca acttaagcag
    34741 gaagaaaaca ctctttcact atggtaaaat aagacgattg aatcaaatag acaggagaag
    34801 aagcaaatgg attttcaaag cattttagaa aagataaaag ctgctttgat cagtttttgg
    34861 cgttggatca gaccatatct cggacaattc catcgatggc gaaagcgcat ctggaaaaaa
    34921 tatcatgtga acaaattgat tttgctgatt ggtttgatct gtgtcttagt gacaagtatc
    34981 tacctcttta ttttagcaaa acaagcaaat gtcgaaacgt taaaatcagg attaagccaa
    35041 tcgacagtca tttatgataa gaaaaacgaa gaagctggca cactgtacgc ccaaaaagga
    35101 acatatgtag aattaaacgc gatttctccc tatatccaag atgcagtaat ctcgacagaa
    35161 gataggaatt tttacgagca tcacgggttt gatatcaaag ggattgcccg tgcagcagtc
    35221 cgaatggtct tgtctggcgg aacatcaggg ggcggaggaa gtacgatcac tcagcagctt
    35281 gccaaaaatg cgtatctgac attagaccaa acgtttgatc gaaaagcgaa ggaattattt
    35341 ttggcaatcg agatcgaaaa gaagtacagc aaagaagaaa tcttgaccat gtatcttaac
    35401 aatgcctatt ttggcaacgg ggtatggggt gtccaagacg cttccagaaa gtattttgga
    35461 gtagatgcaa cgaatgttac gctgtctgag gctgctactt tggcaggaat gctgaaaggt
    35521 cctggtattt ataatccgat cgatcaccca aaaaatgcga atgatcggaa aaacacagta
    35581 ctaagtgtaa tggaagaaaa cggaaaaatc acaaaagaac aagaacaagc agaaagccag
    35641 acagatatca gcgcgtatct taatgacaca tacgataatt cagattctgg ttatcgttat
    35701 ccttattatt ttgatgcagt gatcgatgaa gcagttaacg agtatggtct tgatgaagaa
    35761 gatgtcatga ataaaggcta taaaatctat acagctttag atcaggatta tcaaaagcaa
    35821 ttagaagcta cttatcaaaa cagtgcatta ttcccagcaa atgcttcaga tggtgccatg
    35881 gtccaatctg cttcagtagc tttagatccg aatacaggtg gtgtacaagc actagtagga
    35941 cgaagaggag atcatgtctt tagaggattc agctttgcta cacagatgcg tcgttcacca
    36001 ggatcaacga tcaaaccatt aagcgtatat acaccagcac tggaagctgg atacaagcct
    36061 tccagtatct tgaaagacga accgcaaagt tattacgatg cgcataattt tgacgggaca
    36121 taccaaggag aagtaccgat gtatcaagct gttgcacaaa gtttgaattt gcctgctgta
    36181 tggcttttgc atgagatcgg tttacaaaaa ggatatgata aagcagaaga attcggcctg
    36241 cctttagcaa aatctgataa gtattatggc ttagcccttg gtggactgga aaaaggagta
    36301 tctccattga tcatggcggg tgcttacagt gcatttgcca atgatggtca gatgtatacg
    36361 ccgcatctta tcacgaaaat cgttgattcg actggagcgg tgatcgtcga taacacaaac
    36421 aaaaaaccaa aacaggtcat atcaaaggaa acagccgatg aaatgaccag catgcttctt
    36481 ggtacatttt caaatggaac agcggctgct gcgaatcctg caggttatac gatagcaggg
    36541 aaaacaggaa ccacagaaac taatttcgat gccacaaaag caaatgacca atggatgatc
    36601 ggttacacgc cagacgtagt gatttcgaca tggatgggtt acgaagaatc cagcaagctg
    36661 cactatttgg aagggacaag tggaaatgtt gtcgggaaag tcttcaaatc agcggcagaa
    36721 ggcatccttc cgtatacttc aaaaacagga tttagtgtag cagatgccta tgccacagga
    36781 ggacaagtgg tagctgcaga tcaagttgga aacacaggaa attctaatgg agagtctgga
    36841 aattggcaag ataacttgaa tcaatatggt gaacaagcaa aagaagggct gaaaaacttc
    36901 ggcgatatgg tgaaagaagg cgttcaggga attggtgaag cggctaaaga cctctggcgg
    36961 aaatatcaag gagagtagcc cctttgaaaa cgaataaccc catgttacaa tagagagtaa
    37021 ctactaaaag aaaagagggc ataacatgtc aaacatctac gatagcgcga atcaaatcga
    37081 acgtgagatc cgtgaattac cagaattcaa agcgttgaaa gaagcgtacg agaaagtaca
    37141 agcaaacgaa gaagcacaca aactgttcaa agacttccaa gcgatgcaaa tcgaattaca
    37201 agaaaaacaa atgagtggac aagagttcag cgacgaagat gcaatgaaag cacaagaaat
    37261 ggctgtgaaa atccaatcag aagaagtaat caatgacttg atgcaaaaag agcaaggctt
    37321 cagtacaatc atcaatgact tgaaccgtat catcatgaca ccagttcgcg acctttacag
    37381 cgaataaaac tattttatga aacaacagag ggtgtgaaag agacgttcgg ccttgagcga
    37441 tgacaaaatt gcttcttatt gccgataggc tgtctcttga acaccattta ccaagaggtc
    37501 gtgagcctac cgtttaactc tgagttacaa ggaacaattt caaaaaacag cttctcatgt
    37561 ttgatgacta ttgtgacttg tgaccgagga gttggcagga gaacaccgtt tattcagaaa
    37621 taggagctac gacaaaagtc ttgtcatagc tcctattttt tgtcattcat ttttcaagcg
    37681 gtagaaatag cctttttgag ctaacagttt ttcgtaagta cctatttcaa cgatttttcc
    37741 ttcatcgatc acaacgattt tatctgcctc tttgatcgtt gaaagccgat gagccacaat
    37801 aaaagtagtg ggtgtttctg tcaggtaatt cagggcttgc tggatttttt tctctgactg
    37861 gttatctaaa gctgaggttg cttcatctag caaaatgatt tttggctgac gtatcaacgc
    37921 tcgtgcaatg gagatacatt gcttttggcc accagacagc ttgttgccat gttcgcctac
    37981 taaggtatcc agaccatcag gaagctggtc aatcagtgaa gaaagttgcg cagcttcgat
    38041 gacttcatct aaacgttctt ttgatacgtt tttcaagcca taagtgatgt tctctttgat
    38101 cgaagcggag aacaacaaag tggtttgagg aacaacggct aaataatttc gcacacttcg
    38161 catatccagt tcatccaaag gctgatcatc tagaaaaatt tttcctgaag tcggctggat
    38221 aaatccaatc aataagttaa ggagtgtaga ttttccagag ccagatggcc caacaaaagc
    38281 aattgtttct ccagctttca cctctagaga aaaatgatcg ataatcggtt tgagtgagtc
    38341 cggataaaga aaagaaacat cctcaaaacg aaaatttcca tccaaatgtt ggattttttt
    38401 cttaccgtga taagtttcta catcgccagc aatcaacagt tcgccaatag aagtgataga
    38461 ttccatgcct tttgaaatcg taggaatcag cgtgatcatt ccagaaacgg aattgacgat
    38521 cgtcgtgaaa tacgtctgat acatcacgac atcgccgggt tggatgcgcc cttgtaaagc
    38581 taatatccca gtgaaaatca aacaaatcag ctgaaatatc tgaaaactag cccagctgac
    38641 tgatccgaaa acagattgca ggatatctaa gtggaatcct ttttcataga tttgagtaaa
    38701 atgcttattt aagcgagttg tttcgtgttt ttctaaccca tgagctcgag caatcggtgc
    38761 taattcgatc acttcgatca cttttgcaga agtctcttcc atttcttgcc tgaagtctga
    38821 gttggactgt ctgatccttt tacggaaaaa gagagtaacg aatgcggcga gaggaacagt
    38881 caataagaaa aacatgaaaa caacaggact tttataaagc gtgataccta aagctacgaa
    38941 taaattgaca gcgatattta atccgctgac aaatacttga gaggacaacg tttggatagc
    39001 ttctacatca cggatgatct ttgactggat tcgtccagat tgcaattctt tttgataagg
    39061 aatagatagt tcttggattt tcttgataag ggtcgctctt agtccagctt ctacttgacg
    39121 gatcgaagtg ctatggtatt ttacatgaaa ataattcgtc aatatatttt gaccaatcag
    39181 acaagccatc aaaaaagcgt tgaaataaat ggcctgcaac tgagaagaat ctttcgatga
    39241 agctgcattg attacattgg caatagcgat cggcattaac caagatggaa gatgtttgat
    39301 aataaaaaag agtcctgaca tggcaaaacg ccaataattt ccctgataaa gatgcagcaa
    39361 tgtctgaaaa gcatttgcat gatatttttg ataacttttc tgtattcgtt cctccataat
    39421 cttctcctta acaatcttgg taaataaact gcaagtgttc tattgcttca ggaacataaa
    39481 tctcaggagt agcttcaaga gtggcgtaca ttagaggacg ctcatatttg atataatgca
    39541 gtaccgggcg gaaatctaaa cagcccttgc caacaggtac catgcggatc tttccatttt
    39601 ccatgatata gtctttgaga tgaagcgctg taatataacc atgtaaattg tccaaggcac
    39661 ctcggatcac tttttcctga tcagcatgat tttctttatg catcaaattc gcacaatcta
    39721 aaatgatttt taaatttggt gaagctactt catcgattaa acgtttagct aactgatagt
    39781 tatagattgg atgattgatc cctgcttcaa cagcaacagt tacgccaaaa ttttctgcat
    39841 gagcgactaa ttcaatgata gaatttctgg cgatctgata agcttcttca gtaaaatttt
    39901 cttttgtgta accgacagag acactaccgg tttccgtagc aatcatcttg gcttgatagg
    39961 cagagcacag agcaatttgc tgtttgaaag ttgccaatgc ttgtgcacga acttcttgat
    40021 ttttgctgga taggttgata tagcatccta aaatggaaag ttcgatattg tttttttgga
    40081 agtattcacc aaaataggca gcactcccaa gtgtcatgtc agttaattta agttgttcag
    40141 gaaatgcttt ttgtggtgca aattggatat gggacaagtg aagctgtgat actttttgaa
    40201 cgatttcttc acgatttcga acatctaaat cgtgggcacg gatgcctaaa tttaatggaa
    40261 acatctcaaa ctcctttttg taaataatta gcaagaggca gacgggattc ttttatcaat
    40321 tcaacaatta ttcgggcaac tttatcagcg ccttctggac taaagtgggt attgtctttt
    40381 agtccttctg ggtaattttt acaagtatca ggcataagat gcaggtagaa tttttttgtc
    40441 tcttcttttg agaaggtctg gaacaattct tgtgatcttg agaacacatc taaaagagga
    40501 atatggtgtt tctcagcaaa tgcacgcatc gcttttggat agtctccaag tatatctgga
    40561 ttcaatgttc cgttttctaa gtaatctcgt cgcgtcacag atgtcagtaa gaccatttgg
    40621 acgttcgctt tttttgcgca ttcaaaaaat atctgcagat tttttaaata gctaccgaag
    40681 ggaagcgtac ctctttcttc ttggattttc tggtcgttat gtccaaattg tacaaataag
    40741 aaatcattcg gctgaaactg tgaagctaga cgggctaacc ttccttcctg gataaatgat
    40801 ttggtgctgc gtccattctt tgcttggtta ttgatctgga tctcgggtga aaaataagca
    40861 gagatttttt ctccccaacc tgtctcggga cgcttgtttt catctttttc tgccgcagta
    40921 gaatcaccgg caatatggat agtaggcatg ttatctcttc tttcataaag gtttataggc
    40981 aatgaagcag aacagcaaga gaagaagacg gagcaa
//
LOCUS       DACDMM010000006        40398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDMM010000006 DACDMM010000000
VERSION     DACDMM010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.006"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1414)
                     /locus_tag="IWD16_000237"
     CDS             complement(<1..1414)
                     /locus_tag="IWD16_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9087324.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDI"
     gene            complement(1404..2699)
                     /locus_tag="IWD16_000238"
     CDS             complement(1404..2699)
                     /locus_tag="IWD16_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9087325.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(3031..3708)
                     /locus_tag="IWD16_000239"
     CDS             complement(3031..3708)
                     /locus_tag="IWD16_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9087326.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(3738..4829)
                     /locus_tag="IWD16_000240"
     CDS             complement(3738..4829)
                     /locus_tag="IWD16_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP9087327.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(4930..5364)
                     /locus_tag="IWD16_000241"
     CDS             complement(4930..5364)
                     /locus_tag="IWD16_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9087328.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(5948..7405)
                     /gene="ascB"
                     /locus_tag="IWD16_000242"
     CDS             complement(5948..7405)
                     /gene="ascB"
                     /locus_tag="IWD16_000242"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9087329.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(7402..9297)
                     /locus_tag="IWD16_000243"
     CDS             complement(7402..9297)
                     /locus_tag="IWD16_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9087330.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(9355..11130)
                     /locus_tag="IWD16_000244"
     CDS             complement(9355..11130)
                     /locus_tag="IWD16_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9087331.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(11180..12676)
                     /locus_tag="IWD16_000245"
     CDS             complement(11180..12676)
                     /locus_tag="IWD16_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9087332.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(12870..14492)
                     /locus_tag="IWD16_000246"
     CDS             complement(12870..14492)
                     /locus_tag="IWD16_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9087333.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(14941..16254)
                     /locus_tag="IWD16_000247"
     CDS             complement(14941..16254)
                     /locus_tag="IWD16_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9087334.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(16499..16723)
                     /locus_tag="IWD16_000248"
     CDS             complement(16499..16723)
                     /locus_tag="IWD16_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087335.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(16743..17111)
                     /locus_tag="IWD16_000249"
     CDS             complement(16743..17111)
                     /locus_tag="IWD16_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9087336.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(17309..18019)
                     /locus_tag="IWD16_000250"
     CDS             complement(17309..18019)
                     /locus_tag="IWD16_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087337.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(18037..18534)
                     /locus_tag="IWD16_000251"
     CDS             complement(18037..18534)
                     /locus_tag="IWD16_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9087338.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(18571..19023)
                     /locus_tag="IWD16_000252"
     CDS             complement(18571..19023)
                     /locus_tag="IWD16_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9087339.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(19158..19763)
                     /locus_tag="IWD16_000253"
     CDS             complement(19158..19763)
                     /locus_tag="IWD16_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9087340.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(20213..20833)
                     /locus_tag="IWD16_000254"
     CDS             complement(20213..20833)
                     /locus_tag="IWD16_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9087341.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(20835..21704)
                     /locus_tag="IWD16_000255"
     CDS             complement(20835..21704)
                     /locus_tag="IWD16_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP9087342.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(21727..22134)
                     /locus_tag="IWD16_000256"
     CDS             complement(21727..22134)
                     /locus_tag="IWD16_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9087343.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(22153..23442)
                     /locus_tag="IWD16_000257"
     CDS             complement(22153..23442)
                     /locus_tag="IWD16_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9087344.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(23469..23771)
                     /locus_tag="IWD16_000258"
     CDS             complement(23469..23771)
                     /locus_tag="IWD16_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9087345.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(23796..25889)
                     /locus_tag="IWD16_000259"
     CDS             complement(23796..25889)
                     /locus_tag="IWD16_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9087346.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(26378..27142)
                     /locus_tag="IWD16_000260"
     CDS             complement(26378..27142)
                     /locus_tag="IWD16_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP9087347.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(27253..28122)
                     /gene="mdcB"
                     /locus_tag="IWD16_000261"
     CDS             complement(27253..28122)
                     /gene="mdcB"
                     /locus_tag="IWD16_000261"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP9087348.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(28147..29223)
                     /locus_tag="IWD16_000262"
     CDS             complement(28147..29223)
                     /locus_tag="IWD16_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9087349.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(29247..29840)
                     /locus_tag="IWD16_000263"
     CDS             complement(29247..29840)
                     /locus_tag="IWD16_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP9087350.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(29844..31520)
                     /gene="mdcD"
                     /locus_tag="IWD16_000264"
     CDS             complement(29844..31520)
                     /gene="mdcD"
                     /locus_tag="IWD16_000264"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP9087351.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(31510..31809)
                     /locus_tag="IWD16_000265"
     CDS             complement(31510..31809)
                     /locus_tag="IWD16_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP9087352.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(31911..33557)
                     /gene="mdcA"
                     /locus_tag="IWD16_000266"
     CDS             complement(31911..33557)
                     /gene="mdcA"
                     /locus_tag="IWD16_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP9087353.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(33564..34475)
                     /locus_tag="IWD16_000267"
     CDS             complement(33564..34475)
                     /locus_tag="IWD16_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9087354.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            34595..35452
                     /locus_tag="IWD16_000268"
     CDS             34595..35452
                     /locus_tag="IWD16_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9087355.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(35639..36001)
                     /locus_tag="IWD16_000269"
     CDS             complement(35639..36001)
                     /locus_tag="IWD16_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087356.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            36347..37786
                     /locus_tag="IWD16_000270"
     CDS             36347..37786
                     /locus_tag="IWD16_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9087357.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(37793..40345)
                     /locus_tag="IWD16_000271"
     CDS             complement(37793..40345)
                     /locus_tag="IWD16_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP9087358.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
BASE COUNT        11542 a         8602 c         6770 g        13484 t
ORIGIN      
        1 taatgtcgta aggtgacgca aattctgaca gataatattc acactgtttt ttgtttctct
       61 ggcaaaatgt gatcaccaaa tcatattctt taccttcttc ataacttcca atctcaatac
      121 cgccgattcc cctcagttct ctgatcagat attgttgaaa agcaatacga taaatcggca
      181 gactttctaa atcgattcca atcactactg gttttgcaat ataaaaatcg atcatcaaaa
      241 gaacattggc atactggatc atcaggtaac taagctcact atcttcagct cttttcctcg
      301 gcaattgctg caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag gaatgcccga
      361 gcattttctg ctgctgttcc aaaagccgat cacgctcata gacttcgatc tttccaacaa
      421 agaaaaacca ttcattgttg atctgggaaa tttggtagcc tactgccttt tcttctttga
      481 ttttcaaacg acgaggacga taatgccata acatcgtttc ccgaatgtaa gtatctaaaa
      541 cagcggttgg taactttttg ctccgtgtca aatcatactg ataaaatatt tcttcgtcta
      601 ctatcgaaaa agaaacaaaa aagctgtaaa acatcattgt ttcgtataca ttcagttctg
      661 ctgctgttcg actaagatgc atcacgatga ttggatcgat tgcttgataa agccggtccg
      721 actgatagag taatttcttt tctttcaaag ttgcgtacgt acttttttca ttcaatagtc
      781 tttttttcgt aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc gtaaacgttg
      841 tttctaatac acggtccaat cctagaatga acggtctttt ttctggtgta ttttgcaaaa
      901 aaagcggtcg ctgatcttcc gggatatatt ggaatagttc ataataaaaa tatcgtatct
      961 gtagctcttc tccgtataat tgaccgtttt tgatttgtaa tccaaattcc tctaagagct
     1021 ggttcaattc tttgatcttt cgaaaaacag aagactcact gatattaaac gctgtcgcta
     1081 atcgaacgat ggatacttgc tgatgttcta ataaatagac gagcatctta aacttcaagc
     1141 tttcctgcaa taaaaacaat agaatttttt ccaaacttac atcttcgtcc aacacaagca
     1201 gcgcctgata ctcatttcgc tgtacttcta cttttttttc catcattcgg ccaatcaatt
     1261 caatatcttc caaatattga tcaaacgagg cttttgacag ttggacatgc tctctcaaac
     1321 cattgataga ggctgttccc ccttgcagaa caaggtttcg caaaatcagg atctgtaact
     1381 gctcttttct ttctaataat gcttcaatct tcataggcag tattcgccac ctttgaccac
     1441 accatatctg gcacacggta ttctggctgg aaaaatagct catgttcttc taactgttca
     1501 aacgtccatc cttcttctac tgcaattgtc agttggtcaa gaaggtggaa catctctttt
     1561 ttgctttgga gctgtgctcc aagaatcttt ttttcagaat caaatagaaa tttgagttta
     1621 actttgcttt gatttattgc tgtaagcttg tagtctacgt atcctgtttc aattttttgg
     1681 ggataaaaaa tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata gccaaaccac
     1741 tgattgccga ctggacgata ggttccctga tcctttgtct gtgagccact aattgtcttg
     1801 ctgacatttc gtgctgtacg gacagcgtta tttactagag aaacatacat tttttcattt
     1861 gttggacgaa aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt ttgagacgtt
     1921 tgcaagtagt catctaccca gatcgttcca tcatcattca atgttaattg ttcttcccac
     1981 atctgattgt cggggcgaga atgattagcc agcaaaacaa gatcatagtt ctcctttctt
     2041 ttttccgtct ctacaatagc ggtttcattt tcggtcaaag acacaacttg ttcttctaaa
     2101 aaaagtgaaa gcccttgatt tttcatttcc tttactaacg gttctgtcat ttcaggatct
     2161 agataacgaa aaagaattga ctttcggctt tcgtaaagat ggatttgttt ttttcttgat
     2221 gccaaccctt cagctatttc gattcctgct tgtcctgcac caataattgc aattttcttt
     2281 gcattcatga tcttttgctg aagagccgtt acgttttgta tctttttaac tgggtgaata
     2341 agcgttgatt ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc tagaatcaaa
     2401 cgatcaaaat ccaatttttt tcctcctgct aaaacgactt ttttttcttt caatcctatt
     2461 actgtctttt ctgtgtaaac atggattcga tagcggtcag tcagttcttc tttcgtgatc
     2521 caagaaagtt gtttttcact ggtaaccttt ccttttaata tcaaattgaa cccgctggga
     2581 atccatccta acttcgattg cttttcaatc aaatatattt cacttgttgg catcaattgg
     2641 cgaagataaa tagccgcatg aatccctgca aacgacccgc cgacaattac aatgttcatc
     2701 tgcattcctc tttcttataa ggtgttctac tgctaacttc tcaatcagaa aacacagtat
     2761 tcataactcg ctttcaacct tatgatacat agcgatgcag cagaatcaac cctctccatc
     2821 ataccgtgtt ctaggtctgc ttctcaataa tgtactagtg gacctaattg aagagtatca
     2881 tttctgaaga gatgtttcaa aaaatgtact cttataaaag aaaaaaactc gccttttacg
     2941 tacctctgtc caaataggaa acagaaaaga acgaaaagac gagttttagc gtgtgtaatg
     3001 taatagttag aatagacaca tcatttattt ttaattcttt aatgcttcaa caactgcttc
     3061 gcctaattga tgagcagatt gaggattttg ccctgtaaga agccgcccat caatttgaac
     3121 cacttctgaa aaagctgctc cagactgata gtttgctcct ttttctttca aagcatcttc
     3181 tgctaagaat ggtacttcat tcgttgtacc atttgcttcc tcttcttcat tactgaaccc
     3241 agtcactgta cgtccagcta aaatagattt tccagactca tctgtaactg gcaacaatcc
     3301 tacaacaccg tgacaaacag ctgagaccag cccttcattt cgatagattt gtgaagcgac
     3361 tttcccaaga tctattgctt ctggaaaatc ccacattgtt ccgtggccac cagcataata
     3421 aaccgctgca taatcatttg catccacatc atcaggacgt aaggtatttc ccaaggcccg
     3481 atttctgaaa tcttcatctt cataataggc ccaatcgatt tcatccatat tcaaaacact
     3541 ttgcggttct aaaggtacat atccgccttt aggactgaca aaatctgcat cgatcccatt
     3601 ttttactaat acatcataaa aatgagtcag ttcacttaac cacaaacctg taggacgctc
     3661 gatcttttcg tacttttcta catttgttaa tacgatcaat actttcattt tcccatcctc
     3721 tttctataaa aattgcgtta ttgataacgt gctttcaaga aattatataa tgggcttggc
     3781 aaataaagct ctttatatgc ttcttcaaaa ggtttcgtta caaattcatc ctcacggttt
     3841 tccaataatt tctctgccca gtcaggatcg acgatcattt gctgtccaat tgctgcaagt
     3901 tctgcatatt tcaatacctg ctctacatct tcgcgattgc ggatcccacc gacaccaact
     3961 aaagggatct ttccttgaat cgtgttatgg accacttcaa gaatcgattt atcgttgtat
     4021 ttatcagaac gagctattcg atcatacata tttaatgaaa catgcagata atctagtttt
     4081 gactcacgta atcgttcaag aagccagatc gtgtcttcaa aacggatacc tggtgtttca
     4141 tattcttctg gtgagaaacg ataccctacg atgaatggtt ttttagcata ttgttcaacc
     4201 gctgagaaaa ccgttgttaa taattcttca ataaaacgat aacgattttc caagctaccg
     4261 ccccattgat cctctcgtct attcgaatgc ggagagaaaa attgttggat caaatacgta
     4321 tttgctccat gcaactcgat tccatcaaat cctgattcaa tcgctctacg tgtggctttg
     4381 ccaaatgctt ggattgtttc aacgatttct tcttcactca ttgctcgtgg tgtttccgca
     4441 tcaggacgtt ttgctgcaac tgcacttgca gatacaggct gttctccaga taaagttgct
     4501 cgacttgtca ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc ttctccttgg
     4561 attgctttcg ctagctcact taatcgagga atcatttcat catgagcgat gctgagttca
     4621 cctggccagc cttttccgat atcttggaca tttgcagcac cagtaataac agcaccaaga
     4681 cctttagaac gttgtttata ataagtgatc tcatcttcgg tgatcactcc attgaaaaag
     4741 ctgagttgtg tcgtcatagg cgacagcatc aatcggttct tcaattgaac tcctgaaggt
     4801 aaagtaattg attgatcaaa ttgtgacatt gtcattcctc ccataattcg ctctgattaa
     4861 cctatcgaga atcatctatc attaaaccac tcgattagtt gtacatgcaa ctaatttgct
     4921 taaaaaattt tatttttttt gtaacttatc tgttgcatca ttcattgtct gggctaattc
     4981 tttacttgct tcatttccca aaactgcttt gtatgactga tgaagttctt cccagctacc
     5041 atcgacttcc ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg acaagcgacc
     5101 ttcgctaact gttttgacgt atcctttata ttctaatttt tcaacaaaac gagcaatcgt
     5161 tgatggggcg gaatagagca tttgtgcaat atctttttgt ggtattccat ctttttcttt
     5221 taatatcaat aaaataaaac cataagttgg tgccagacct gtccttctaa acgcttcatc
     5281 agctaattta ttcaacatgc gatctagctt tttcacgcta aagaaaagac attcatctaa
     5341 cgctttttca attggctttt ccatttcatc ccctccattg gcgtacatat catagtatga
     5401 tttttatttt ttgtcaaaaa acacggaata atgtccgcct cctctgtcta gatttgaaaa
     5461 aaagaagtag ccatcttcaa agaatttcgg cttgttttcg acaaaactcc ttctggaaca
     5521 ccgtgaatca gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa aattttgtga
     5581 aaatggcttt ccacattttt gcataaattt ggcttaatac cgaaaaactg gcttttgaac
     5641 accgtttata tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag aacagccaaa
     5701 caaaaatagt tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc agctgattgt
     5761 acaccgttta ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa ggagcaattt
     5821 caaaaaacag cttctcatgt tttatgacta tggtgacttg tgatcgagga gttggcagga
     5881 gaatactgtt tattcgtaaa gaaggggctg tgacaagact tttgtcacag tcccttattt
     5941 ccttttatta taggatttcg ccatttgatt ggatgacttg tctgtaccaa tcaaatgatt
     6001 ttttctttaa ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca tagatatatc
     6061 cataacgttt tgacatttgt gaagtagaag cgctgacgat atcaatgcag ccccacgacg
     6121 tataaccaaa taaatctact ccatctttga tagcttcctt catctgctca atatgctgtt
     6181 tcaaatagtc aatgcggtaa tcgtcttgaa cggttccatc ttcttctact ttgtctatag
     6241 ctccgagtcc gttttcagct atgaacagtg gtttttgata cctatcatat aattgattca
     6301 aagcaattct caaaccctta ggatcaatct gccagcccca ttcacttgat ggaagatacg
     6361 gatttttaat tcctccagtt attaagtttc cttggacccc ttcccaattt tctggatgag
     6421 cactgactgc actagacatg tagtagctaa aagaaacaaa atcaacaggg taggctgcta
     6481 agatttcttc atcacctact tctattttta agttgacacc tttttcttca aacattcgtt
     6541 gcgtatacgc tggataatat cctcttgctt gaacatcact aaagaacaaa gaagttcttt
     6601 gctgctcaat tgttttttga atatcatccg gatcacaaga atacggaaca agaagctgat
     6661 agctgatcat acaaccgact tgtgaacctt tgataatttc atgacaagct tttgtagcta
     6721 aagcacttgc tataaactga tggtgtgcac cttggtattt cgcttgttcc aaatggggat
     6781 ggttttcttc aattactcct acgctaacaa aagggtgatg ttttacacag ttgatctcat
     6841 tgaaagtcat ccaatatttt actttatttt tatagcgttt gaatactgtt tcagcgaaac
     6901 gaacgtaaaa ctgaataagc tttcgattag gccatccacc atattctgta accaaatata
     6961 acggcatttc ataatgggaa agagaaacga ctggctcaat cccatatttt agacattcgt
     7021 caaacacttg atcataaaaa aataatcccg cttcattggg tgtttcttca tcaccatgag
     7081 gaaaaattct tgtccatgcg atagacatcc gatagcattt aaaacccatt tcagcaaata
     7141 aacggatgtc ttctttaaag tgatgataga aatcatttcc gtgtctttta ggataataat
     7201 acttgctgtt gggatcctgc gccgctttta cttgctgatg cgtcatcata tgccattgat
     7261 ctgtccattt ttctttaggt aaatcgctgt cgaatgtata cacatcagac acagataatc
     7321 ctttttcatc tacatcccat gcaccttcac attgattggc cgctgtcgct ccgccccata
     7381 aaaatccttc tggaaatttt ttcatatcga tccccctaaa cttgctagtt ttcttgtttg
     7441 attatttttc ttatgactag taatgatatt tttgaatcta ctactatttg taacaataac
     7501 cattgtggtc ggatcatatc cctctttgat taattcttgc aaatctactt ccatcaataa
     7561 atcgccttta cgtacttttt ttccatcaga tactgcactt tgaaagtatt tgccatttaa
     7621 attcactgta tcaataccga tatggatcag tatctctgct ccttcttcac tgactacccc
     7681 aattgcatgt ttcgttggaa aaactgttga aacttccccg tttacaggac tgtagatttt
     7741 tccgtccgtt ggaatgatcg caaacccttc tcctaaagat ccactagcaa atgttggatc
     7801 gtttacttca gataaatcaa ttatttcacc tgctgcagga gtacttaaca ctatatcctt
     7861 tgaggattct tcttcgattt catccttgta gccgaaaaga aaagttagca tacaggaact
     7921 gatcatcgct aagaaataac tagcaactag ccaaacaaat ccttcaccaa tataaatcgg
     7981 gaaagacaaa atacttcttg tagcaactgc caaagcaccg gctcctgcgg caccagctac
     8041 tccaccaaat atagctccac atatacaagc aatgtaaaat ggtcgtttat atttaagagt
     8101 cacaccataa acagcaggtt cagtgattcc aaataatgct gtgataaatg cagataaaga
     8161 taaacttttt actttcttat ttttcgtttt caaaaatacc ccaagtactg ctcctgcttg
     8221 cgcagctacg gcagtcatac atgctggacc tagcgtatca cgaccgtaga aagaaagatt
     8281 gttggtcatt actggtacaa gtccccaatg taacccaaaa atcactaaca cttgccaaaa
     8341 tccgccgata acagcaccag ataaaacagg gctaaagtta taaacagcta aatatccagc
     8401 tgcaatcagc tgacttgcat aatctgcaat tggacctaca actaataaag taattgggac
     8461 catcacgatg atacaaatga atggtgtcaa taaatttcga atactttcgt gcaagctttt
     8521 gcgaatctta ggctcaatat aagaaagaac ccatacagct aaaattgctg gaataaccgt
     8581 acttgtatac cttgctagaa tcacgggcat ccctagaaat cgcaaggtta tgctatcact
     8641 atatgcactg acaatcgtag gataaacgag agctgctgca ataaccatag cgatgaaacg
     8701 atctgtattg aacttttttg cagcagtata tgccaaaaat ataggcaaga aatagaaaaa
     8761 tgcatcagct gctgcattca aaatgatata agttcctgat tcagtagtta ataaattcaa
     8821 actcgtacat aaggcgagta ctcctttgat caaaccactt cctgccaaga cgcctaaagt
     8881 cggggcaaaa acagcggcga tcatgtctat aaaagagttt aaaatgcctt ttttctcagt
     8941 tcctctcttt ccactagatg catcatcttc tagtttcatt tgtttggata atacgtcata
     9001 cacttcattt actgcatttc ctactacgac ttgaaacatt cctccagctt cgactacagt
     9061 gatcacacct tctagttgct ctaccttctc cttatctgcc ttcttattat cttttagggt
     9121 aaagcgtaac cttgttgcac aatggtaaac attgctgaca ttttcttcgc ctccgacaaa
     9181 tcggacaata tctttcgcta attgtgaata gtccattttt acttccccct tgttaattaa
     9241 tgccgtagca atccttctta caagataagt atagttctta gaaaacggtt tctccattca
     9301 attagtaccc cttataatag cgaacgaaac tgtacaatag tgtgtatggt ttacttagcc
     9361 gagcaaatca ctcactgctt gaaagaaaac aagtatatcg tttctcttat agtcaaagga
     9421 gacaaaaaaa acacgcttga tcttcctttg ctgatgtata agtggtatct caagctgata
     9481 ctctgtgatt tttgtttcta cttggctttt gataagacag cacaacaaag tctcggcatt
     9541 taacggaagc ataactaggt cagaactagg agagattagt tgctgaatgg aatctatctt
     9601 tttatggatt tttatttgat tttcttcttt gtagtaatga tcaagcagtt ttgttttctt
     9661 tttttcttca actatggtca gcttttcacg caaaatctga taaatctccc aagattcaaa
     9721 ctcatctaga acactaggta gataaatcaa atcatcccac tcaaaaataa gctcttgctc
     9781 agtagtcaaa attgcatcat attccgttat tttgtttgat tgatttttga aaagatacac
     9841 tggttctatc cggcattctt tgatccacat gttcatactt tgttgaatct gtttttttag
     9901 tgtattcaaa atactaattg gctggttact aacaatcagt atctcccctt gttttcgaaa
     9961 aggattcaac gcttcggcaa gatacaaaac aaaataagcg ctttcagcta aattgggacg
    10021 taattgaaaa tgtattggga agaaacgccg aattaaataa atttctaaag gataacggga
    10081 gatgatcttt tgtacaaaat gattcatggt attatgtccg gcatccatcc tcgttttcat
    10141 ttgtttgata tgggttagca aaaattctgt ttcatggaac aataacgaaa caggaagatc
    10201 taaaaaacga ataatcgttt gctcaaatgc tcttagattc ttctctattt ctttgttttt
    10261 ttcatcatta atcatcaaat aatttaactc taatagtcga ttttcaattg cttgtttttc
    10321 ttgattggaa aaagaatagg gtagttctga tgataacttt tttgtgaggg tctctaccat
    10381 cggaataaat aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc gaagaatggt
    10441 caaaacgaga taccgagaaa atcgtatgaa gtcctctcct gaaacaatat aattatatga
    10501 tataagaacc tcttttaatg tcttttggac agtcggcatg atattggaaa aaattgtact
    10561 gatttgttga ggcaaccttg cgtgttggat caccgtttct gttccaacaa gagataagaa
    10621 aattcttttt gaattttctt cagcaatgat ttttagtcct tttgtcgacg aaacttccag
    10681 ttctaattta ggattgcgct cgatccaacg caaaatcgtt tttatttgta atgatacggc
    10741 tgtttttgaa agaaagaaaa tgtctgctat cttttgcata ctgacatacc ctttttgaaa
    10801 caataagtac gtcacaatag ttgaagctcg gaaatccatg ctggaataga ttttatgttt
    10861 cgtcacctct tgtatgattt gctgtttagt ttcagattca attgattcta aaaaataacc
    10921 tgaacgttta tttgactgga taatgatttt cttctcgtag atagcaagca tatttttgat
    10981 ttcagtttgg atcgttttct tgttggtacc taaagcttca gctaattctt cgcttgttat
    11041 ccaatgattc ttatttctta atactacaag aatttggagt tgtcgtttat ttaagtccat
    11101 ttgcttacac ctcctctttg ttataaacat acaggaaaaa cctctcaaat gacaatagtc
    11161 acttgcagag gtttaaaaat catttgaaga aagatctata ttcaaggttt ttaccattag
    11221 actggataat ttgtttataa tattcaaaac tatctttttt caatctccta aagcttccgc
    11281 tcccatcatt ttgtctatcc acataaataa agccatatcg cttcctcatt tcccctgttc
    11341 ctgccgatac gatatcgatt ggtccccacc ataagtaacc aatgatttgg caaccatctt
    11401 ttattgcctc agcaatttcc tgaagatgta tttttagata ctcacaacgt tttggatcgt
    11461 ggattcgatt caattcatct ggtgcttcat ctagtccaat gccattttca actatgaata
    11521 atggtaattc ataacggtct gtcaattcat tcatgacata acgaaaccct agaggatcaa
    11581 ttggccaact ccaaggttcg ggagattttt ccttcaaaaa gggattatcg aaaccatttg
    11641 ctccgccagt atctacgcgc atttctgctt cttttttata gacagcgcta cgataataac
    11701 taaatgcaat ataatctgcc gtgttagctt gcaagcactg ttcgtctcca tctaacatta
    11761 ctggtgcaca gtcttcttcc ttccatgtcc tgtaaatata agctggataa tgccctcggc
    11821 acatgacatc ggagaaaaac catgaattgc gtttaaattg cagtgaacca aatacattct
    11881 ctggatcaca gtcatatgca taagtcgcta atgcagaaaa gctacacata atacccatct
    11941 tagcatttgg atcgattttc tttaataatt gagtagcacg ggcgttcgct agaaacatat
    12001 gatgacaggc ttgataaatc actttttttg ttagtgtagc aatgggttca agtttatctc
    12061 tagcagggaa tgtaaccaat ccagcagctg cataaggcat acgaaaagca ttattgatct
    12121 cattaaaggt catccaatat ttcactttcc cttggtagcg ttcaaagacg gtctgcgcat
    12181 aaaattcaaa aaagtcgatt aattttcgat tttgccaccc cccatactct cctaccaatg
    12241 ctaagggggt ttcataatga ctaagagtaa ttactggctc gatatcatat cgatttaatg
    12301 tattgattag tctgtcataa aatacgagac cagcctcatt tggcttctgt tcgtctcctc
    12361 ttggaaatat tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct aactccttca
    12421 gtaaacgaat atcctcttca aatcgatgat aaaaatcaat cgcttcatga gaaagataat
    12481 ggagtgattc gtctaaagca atctcccaaa tctttttttc ttcattccac tgaatactag
    12541 gatgctggtt catgatcccg ataagcgtat ctgtcacatt aggtaatttg ccgtcttcca
    12601 accatgcacc ttcacattga ttagcagcta ctgctccgcc ccataaaaaa ttttctggaa
    12661 atatttcctg atccataact atctctcctc gctatcaaca atttcttact tgaagtatag
    12721 ctcgattcat caaaacaaga tattctaact atttcggcag aagagaagaa aaaaattgaa
    12781 tgctactctt catttttaat atttatatat aacaaataca taagacgatc cccgaaagaa
    12841 aacactcgtt aaccatctaa gcaaacgatt tattttactt ccaacacaaa tgcttcataa
    12901 ggtcgtaaat caactttgcc gtctggtaac tgtttcagag gataatttga cacaataacc
    12961 cttaaagctt cacgtggaac agtgaacgtc tgcttttcac tggacatatt acaaataacg
    13021 agccattgct ggtcttcata tatgcgagaa taggcaaaga catgctcagg cgtcttttcc
    13081 actaattcat agtctcccca gacgatgatg tcatgttttc tacgcaaatc gatcaatttc
    13141 ttgtacatat aaaaaacaga atcttgatct gctaacgctt cttctgcatt gatttcttta
    13201 taatttggat tgactcgaag ccatggaatt ccagtagtaa acccagcatg ttcacgggcg
    13261 ttccattgca taggcgtcct agcattgtct cttcctttcg cattgattga ttccaagatt
    13321 tcctcttttg taaatccaga agaaatcctt tcgttataca tattgattgt ctcaatatct
    13381 tttgcttccg cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc tccttgatag
    13441 atataagggg tgcctttcat catatggagc aagatagcaa acatcttggc actttctttt
    13501 cgatacacat cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg attattccaa
    13561 aataaactat tccatccttc atcacctaaa gatgtttgcc atttggataa tacttttttc
    13621 agttcagaga tactcaaggg tttcaaatcc catttgtctt tgccttcttg ctgatctaat
    13681 cctatgtgtt cgaattggaa gaccatggat aattcatttc ttttaggatt ggaataaagt
    13741 ttagcgattt cggtagttgc tccccatgtt tcgccaacag tcatcagatt tttgtcacca
    13801 aaagttgctt gattcatttc ctgaagatac tcatgcagtt taggtccatt acttgtgatt
    13861 tctttatccg gttctttgcc aatcatatcg atcacgtcca tacgaaagcc cccaatacct
    13921 ttatcgatcc agaaattcat catttgataa atctcttggc gaacctgttt atttttccaa
    13981 ttcaaatcag gttgacgctt gctatacaag tgcagaaaat attgccccgt tttttcatcc
    14041 aactgccaag cagaaccact gaatattgag cgcaatctat taggctcttt tccattgacc
    14101 ggatcacgcc aaatataata gtcccgataa ggattttctt tatttttacg ggcctcaatg
    14161 aaccacggat gttcatcaga agtatgattc accaccagat ccatgatgat acggatattt
    14221 cttttctttg cttcttcaat cagttcttcc atttcttcca tcgtaccgaa ttctgccatg
    14281 attgcttgat aattactgat atcataccca ttgtcatcat tcggagactc gtatactgga
    14341 gaaagccaaa ttgcatcaat acctaaatac gccagataat ccaacctgga aataatcccc
    14401 cgcaaatctc caataccatc ttgatttgaa tcttgaaaac ttcttggata gatttgataa
    14461 acaactgaat ggtgccacca tttttgttcc atgattttcc tcctataaat ggatgtttct
    14521 attttttgct taatcattta ttttagtctc tctcactaaa aattgtaacg ctttcaaata
    14581 gctattgcac cttgttatcg gtaacagaat aaaacaaaaa aacagctgag gaaattccct
    14641 caactgtttt ttgaaaggat tgatttatgc agggaagaat gctgatttca caacttcttt
    14701 ataaacggtg ttcactcagc tgtcccttgg tattagctca aaaaacggca aaatatgagg
    14761 aactattttt tcgttttttt ctctaatact caggccagaa cactgatttc acaacctctt
    14821 tataaacggt gttcactcag ctgtccctcg gtattagctc aaaaaacggc aaaatataag
    14881 aaactatttt ttcgtttttt tctctaatac tcaggccaga acgctgattt cacaacctct
    14941 ttatttcaca ttcaatatcc acatgaatcc cacaaataca aagaacagac catacatgat
    15001 tggagatact tcttttcctc ttttagctgc taacatggta attggataga agataaaacc
    15061 taatgcaatc ccatctgaaa tactataagt caaaggcatg ccgatcaaga tcaagaatga
    15121 agggatcgca atctctaagt ttccccattc gatttgtcgc aaagattgtg ccatcaatac
    15181 tcctaccata atcaatgctg gtgcagttac ttgtgaagta acaactgata gtaatggaga
    15241 aaagaacaag cctaacaaga agaaaatccc tgtagtaata gctgtgatac ctgaacgtcc
    15301 tccaacggcg ataccagctg atgattctac aaatgcacca acaggagaag taccaaataa
    15361 ggaacccgca agcatagctg ttgaatcaga agctaaagct ttccctacac gaggcatctt
    15421 gttgtctttc atgaaacctg cttggtttgc taatccaact aaagttcctg ctgtatcaaa
    15481 gaaagtcaca agcaagaatg tcagcactac gacccacatt tgaagagaat tgatgtcccc
    15541 tacatgtttt aatgcaacta aaaatgttgg tttcaaactt ggtgctgcgg caacaatttc
    15601 agaaggcatt ggaatcaaac ctgtgaccag acctaaaatc gttgtagctg ctatcccgat
    15661 gaagatgcca cctggtacac gccgaacaag caagatagca gttacgacta atccaaaaat
    15721 cgtcaaccaa gtagaaccaa cgttgagtga tcctaatcct actaatgtag attcattcga
    15781 aacgatgatc ccaccttcgc tcagaccaag gaaagcgata aatagaccga taccgccaga
    15841 aatcgcatat ttcaaatctg ctgggatcgc atcaatgatc atttcccgca gcttgaagat
    15901 tgtgatcaaa ataaaaatca aagaagcaac gaatacgccg gctaatgctg tttcccatgg
    15961 aatccccatt cctaaacaaa cagaataagc aaaaaaagca ttgatcccaa gagcaggtgc
    16021 cgttccaatt ggataacggg ctaatacccc cattaaaaca cagccgatcg cactggctaa
    16081 tgcagtcgca gtaaacactg ctccttcatc cattccagaa gcacctaata cagttggatt
    16141 tacaaataag atataagcca ttgagataaa tgttgtaaat cctgccagta cctcacgttt
    16201 taccgtggtg ttcaattttt ctatttcgaa gtaggaaagt attttttcct tcatcttcca
    16261 tccccctaaa tccgaacatt ataaataaag tccgttaaaa tttcgattta ttaataaaac
    16321 aacgtgtttt atcatatact aaaacaaaac ataagtaaac aaaaaattcg gattttaagc
    16381 aaaatccgaa cgttccatct gattaaatcc ctttatctca aagttcatga acattcactt
    16441 tggattacaa gaatttcttt attgtgttaa taaagtttct tcattctagt ctcaaaaatc
    16501 aatagtgtga tttgatttta ccatttcgtt tctgttcttc tttgatgaat tcttttgtat
    16561 cgacaggatt cagattattt agtccgccaa aacctgtatc gaccattttt ccccagcctt
    16621 ttgtcttctt ttttcctgat ttgagattct ttgctcgaag cttctcacta tgttttgctc
    16681 gccgatactt caaataacct tctaatcttc ccattagatc cactgttttt cctccttttt
    16741 attcaatcag acgactcagc ttttcctata acaaaataat tgccttcagg gtcagaaaaa
    16801 ttgaaagtca agatgccatt gttatctgtg atttctcctg gttcttccat gttctcgtag
    16861 atagtttcaa attcatccgt aaaaaatact aaagaaggcg gtggtcctaa tacctctggt
    16921 gaaaacttct gcacaaatac ttttgggaat acgcctaatt ctaattcttt tgagatgctg
    16981 agtacaatac tcttaaattc ttctggcaat tcgattgttt cagaaatttc tgcaccaatt
    17041 ttttctacaa agaatcggca aatcatctct acgtcattga cataaagcat cacacgtgtt
    17101 ttttgtatca tcatttgacc cttctttcta tttgattttc tttcctgtct ataaactgat
    17161 tccattatat cacgaaagcg caaacacctt gtgtttgata ctattcaaga aaaataaagc
    17221 aatagtttct aggattgctt tcctccacaa acttttttta caaaaaaaaa aaacggcaat
    17281 gaatgccgct cttcttattt cgattctttt ataattctgc ttctgattca tctttggtta
    17341 gtgtgtgtct aacactagtt acgccaaatg tttgatcgat tttttccttc aacgcttcga
    17401 ttgcctgtgc gccacgtgcc gcttgttcag cattgatgga ttcaatgacc agcaccgctg
    17461 cttgtgtttc ttccgtcagc attgttcttt gggcttcacg ccagttgaga cagcgacaga
    17521 tcgctccttg ttcatcatag tagatgattt cttccggaag tgccggagca tcggaatctg
    17581 cacctaaagg caggaatgat tcgccacctt gggcttttcc taaatgcaga tttcctgcga
    17641 tcttttcgat atcttctccg ccgcaaggta cgccaaaaga caaggaaata ctattataga
    17701 tatctacaag cggattgatt gggaaaaatt cccgttcttg atttgcacgt ttcagtaaag
    17761 cttcaataga agaacgtgca ccttttttag tcttgaatgt ggtaaatgcc tgacgccact
    17821 gagcaatgac ttcgttttgg ctgaatgttt cttctgtaag gtaatttctt gcttcttttg
    17881 ctgcctcatt caataattga tgatacgttt cttcactttc ttttgttacg tggttatcga
    17941 taccatttac ggataagatt tcgatggttg cttcaggaaa tacttcccag aaattggtat
    18001 ccactattac ttttttcatc tgttcacacc tcttgattag aataattgct tgatgaatag
    18061 atagtctgtt tggggatcat ctcccatata aaaaacatgt tcgctgtatt tgataaatcc
    18121 ttttttacga taaaaagcga gcgcaacatc gttgtgttcc caaacaccga gccaaatggc
    18181 tgttttttgc ttttcttttg ctttttcaat agctgtatct agtaaaaatg taccgatacc
    18241 ttgatgttta aatttttttc gtacatagat tcgctcgatt tctaaaaaat gatcagccat
    18301 ttcctcagat tgactcgttc ctgtatttag tttcaggtaa cccgcaggct cttgatcgat
    18361 actcactagg aaaaaatcgg tttctttatc ttttagctct tctgtaagtt tttctagtgc
    18421 ataagcttgt gtcaagtaat cagctaaatg ctccggtgaa ttatcttttc caaatgtatc
    18481 tgtaaatgtt tcgatactaa tttgttgtag taagtctaca tctgaaggcg tcatttttcg
    18541 aatttcgtat ttattcacgg cttcttcctt tcaatagatc cttttattgc cttttttgac
    18601 aaaattccaa tcttcgctaa cattcttgca tactttattt aaaagatatt ccaattgttt
    18661 tgcttcttcg tcagaaagac catttaatgc gactgcattg gagtagttgt tttctcgaat
    18721 gatgaaagga tagatttctg ctcctttttt agtcggatag agtttcttga tttttttatt
    18781 ttctttatct tccaaacgtt cgatcatccc attggattct aatttcttga ttgctcgggc
    18841 agtcgttgta cgatctactt tgatcatttc tgctagtttt tcttggatga ttccaggatt
    18901 ttcgcaaata cgtactaaat aaagatactg tcctcttgtt aaatcgactt ctttgaattc
    18961 gatattgcta atagaatcta gcgctcgggc aatcattccg atgtctcgaa ggatttcagc
    19021 catttcatcc cacctttatt ttgtttgtaa gactagttta cctctttttg ttgcaaatgc
    19081 aactattttc aataaaaaaa tcaagttctt ttttaataac tagtaaagta tattcttttc
    19141 ttcttttacg aaaaagctca atcacaatag atctggtttt tgttttctaa aataagtaac
    19201 tgtttcttcg ttgttcgctt ttctgatagc tttggcaact cttctatact aaaaaatccc
    19261 atgttggaag tttcattatt ctcaataaac ttggcctctt caccgtggat cgcacaagca
    19321 aaaacaagtt tataatactg aaaaagctgt ggaatatctt tttgtttatt cgtatcaaaa
    19381 actgctcgca actgagtagt ttctactgtt aatcctgttt cttcatagac ttctttacgc
    19441 acattttctt caggagacaa tcctatttca gcaaatccac caggaagtga ccattctttc
    19501 gtccgtttat cttcgattag taggaccttg ccatcttttt tgatataggc acgaacatct
    19561 acttttggtg ttggatgtcc ttctccaatc gttgtgagcg cttttatttc ttcgaaagtt
    19621 tcttttccta tttcactgat cagttccaat gaaatcgtcc ggatctcctc aaaacgctct
    19681 ttatcaaata catcttttcc ataatataga ccagcttgag aaagtgcgag tagtcttttg
    19741 tattgagcca acttcttgtt cattctcttc ccttcttttt tagcaaatgt atgactatgt
    19801 aatcaagtat agaagactgg tttaattacg gcaaataaat ataatgaaat ctgcaataaa
    19861 taaaagaatg ttaaaaaact cgagaacaaa agacattcga cttctctggg gctgtgaata
    19921 aatggcgttc aaaagcttgc atctgcggta ataagcccca ccaaacgaaa aatatgacga
    19981 gctattcttg tctggctgtt cttattaatt gaggcaggac agctttttca caacctcccc
    20041 actaaacggt gttctcctac caactcctcg gtcacaagtc acaatattca tcaaacatga
    20101 gaagctgttt ttttgaaatt gctccttgtg actcggagct gaacgacagg ctcacgacct
    20161 ctcggtaaac agcgttcaaa agccaatttt tcgatattaa gccatttctt tgttacttat
    20221 tgtcattgat ttctttttct aatttatcca atagtggagt gtaatccact tgagtatagt
    20281 ctgttacatc aaattcatat agtgttccta actggaattt gtcatatttt cggtcggcga
    20341 tgatcttttt gaatgcttct ttcgtaatat gattggtccc tttcgttcga tcttccaata
    20401 cgtcaccata gtgataatga tccatgatat agctcaaatg acgatctgat tcacgatcac
    20461 gtttgtaccg acgttgacat aacacttcaa agtctgctgt taggcgaatc gtgatcacct
    20521 gatatccgta tctatctgct aattcttgga gtcgtgcttt ttgcttatca ctaaaaggat
    20581 actcggacac aatcactttc tttcctgctt ccatatacaa atcaagtgct ttgtaataga
    20641 aaggataaac tttcgtcctt tcttgttccg cccgttcttc caaattgtta aacccgatgg
    20701 attctgcata gtattccttg atttcatcga gcgtgatggc atacatatct ggaaatcgtt
    20761 cccgtagttt attcataaga tatgtttttc ctgtaccagg actaccagcc agaagaatga
    20821 aatacttttt catctcattt atttcctaag ttaattggac tttccaagtt cataaacagt
    20881 ccaatcttat gcttgacaat ttcttttact gcttcatttg ctggaatcac attatggcga
    20941 agagacttgt tgatttctgt ttcactaaag gtttctttag ctactttcgt ccagttgact
    21001 aaaagctccg ttccaacatt gaatttgcgg atcccgttat tgatcaattc aggataatct
    21061 tcttctttca cacctgttcc tccatggata actaggggga catcaactac tgcatcgatt
    21121 tctttcagca agggaatgtt tacttctgtt tttgatttga attgtccatg attcgtaccg
    21181 atagcaatag ctaatgcatc cacgcctgta gcttctacaa atttcactgc gtcttccggt
    21241 cttgtatata ccttgtcatt ttcagcgaca tggattcctt cttctgtacc gccgattgtt
    21301 ccaagttctc cttctacgga aacaccgtgc gcatgggcat attctactac tgcttttgtc
    21361 ttcaggatat tttctttgaa aggcagatgg gacccatcat acatgacaga agtatatccg
    21421 ctgtcgattg cttctttgat atcatcaaag tctcgggcgt ggtctaagtg caagactgca
    21481 tcaaccattt cttcttctgc aatagctgtc attacattga ctaacacatg catgccgata
    21541 tatttagcgg tatcgacact tgtttgaata atgatcggcg aacccatttc ttttgctgcg
    21601 cctagcatag caggaagcat ttctaggtta tgcgtattaa aagctccaac tgtaaaattc
    21661 aactcttctg ctgtttttgt tacttctttc aatgttgtat acatctttta ttgccacctt
    21721 atctttttat ttgtttcctt tgatcgttac ttgttttgca atttgtcgca tctcatccat
    21781 tttgcccatg tagtaatcga tcccttgatc atcaccagca cgagcagcat ctgcacgctt
    21841 ctggttatag aggctcatca gttttttata cccatctccg atcgtctgtt tattttccaa
    21901 actcttttct aaatattgga tggcttgatc gttcacatac aattcagaaa atttaagtcc
    21961 aacttgttca taaagtttcg ctaatttatg cttatcttgg gtagtggaaa tctcttgttt
    22021 caattgtgta atctctgatt gaatcttttc ggtcgcttct ggcgataggg ctttttcttc
    22081 ttttgccaga acttttttcg gttctttttc ttttttctta aaaaatccaa acatcatttt
    22141 ccctactttc aattaaaatg ctttcaaaac tgggcttaag atccaggcat ataagagtgc
    22201 tgcggctact gtatctacat cggttgctgt agcattgatg aatcccatcg aattaaagat
    22261 cgttactagc aaagcgggca ataatgtaat aaagaaacca tgagcaatac cgccaatgat
    22321 cgctcctcgg cgtccgccaa ctgcgttgcc aaatatccct gctgtccctc cggcaaagaa
    22381 attggttagc ataccaggta aaatcatcgc taagccgaac atgggcaaag taaacatggc
    22441 aataatcgta ccaatcgttg tcgtaatgaa acctaaaatg acagcatttg ggctataagg
    22501 aaagaaaact ggacaatcta aggcagggat cgcatcagga actaatttca tagctatccc
    22561 tcgaaatgcc ggcacgattt cacctaacag tagacgtaca ccagctagca atacatacac
    22621 acccactacg aattggatgg cttgtaaaaa ggcaaacatg atatagtttt gatctcctga
    22681 taattcagaa gcgaatggtt cgcctgcaaa taaaacagtg atgatatata gcgggaccat
    22741 cacgaccatg acagacaaat aagtatcttg cagaaattcg aaatgtgtcg gcaacttgat
    22801 atcttcgatc gactttttat tttcgccttt ctcaccaaaa agcttagcaa caccggcttc
    22861 aaataagtac ccgatcgtac aaaaatgacc taaagcaata tcatttgatc cagtgatttt
    22921 acgaatgatc ggttgcgcta ctgccggcat agcaatggcg aaacaagctc cgataaaacc
    22981 accgaccaag atcaatacga tccctcgcag accggcaaaa tagccaaaga ctgtcgtcat
    23041 cgttgccatc cataaaattg cttgaccagt taaaaaaatg tacttccatt ttgtgaaacg
    23101 agccaaaata atattgaata caaaaatggc taagaacgtc agtgcaatat ctctacctaa
    23161 acccaaatca ttcattgctt gaccattgat agattcaata gaagggatga tcccttgcat
    23221 atgaaatcct tcagtaaaga ttttcccgaa ataagttaaa cttccaacga tgatgctcga
    23281 accggcactc aacacttgaa atcctaggag tgtctttaac gtgccagaga tgacttgtcc
    23341 tgctgatttc ttctgcagta gtagacccaa catagcaatc aatgcaatgg tgatcgatgc
    23401 ctgagtcaaa atattttcga tgataaaatt gataacgccc atgtttttac cctcttttct
    23461 ttttgtattt agataactcc cttatcttgc agaactgggg tgattttttc ttcaatttca
    23521 ttttttgaaa caatattttt caaatagatc atcgccgttt taggatcaac tgcgaacttt
    23581 tcgaactgcg aacgaaaatt ttctgctgtg atgatcacat ctggtttcat ggaaactgcg
    23641 gaagaaatat cacaatggtc cagtttggct tctactccta atttgttcaa aacatcctca
    23701 gcagacatct gtgctgcaaa actacttcct aaacctgcac cgcatacgaa taaaatgttc
    23761 aatttgttca tctttgttct cccccgtatc atttattatt tctcaactac tgaaaaaagc
    23821 agttgtttga aatcttcgat atttgtgcat tcaatcaatc gatccaactt ctcttcgttg
    23881 ttgatcaatt caattagttc tttcatgatg ttcagatgcg agtaggagtc tactgctgcc
    23941 aaacaaaaaa taatctttac tggatccatt tcaggattac cgaaatcgat tggttgctca
    24001 atcgtagcta cgctgactcc taatttattg accccatctt ctggtcgggc atgggctagt
    24061 gccatgtgtt tgccaatcac gatatacggt ccgtaatcat tcactgcacg aatcatcgct
    24121 tcgacatagc gctcttcgac aatgttttct ttaaccaatg gtctagcagc tagttcaatc
    24181 gattcctccc aagtagtggc tttttctttg atcatgatat tgctgtccgt caaaatatct
    24241 tttaacatag gctggatctc ccttttgttg atttctaaat tgtttgtttc gaataatttt
    24301 tctaacttgc catagatgat tcgatctact cggccaccac tttctttcat gacatctatt
    24361 atttgagtga ataactccgt cgattcatga ctgttaggct ctaaccgctg tagttcttca
    24421 tttcttttca aaaagtcttc tataaccaag atattcgtct cagttaaaat cggatcgaca
    24481 actaaaagcg gcttatttcg ataattcaat tgaaaggtgc tgaaaaccag attgacatcc
    24541 aatttatcta ctaattcgat ttcttttgaa cttaaaactg cagtaatctc gatctgagga
    24601 aacttttctt tcagattctc tgctaacaga tttccagtag ccatcccatg gttacagaca
    24661 acgactgatt tataaacata attcaagtct cgcttgatta cactggcgat cgtcgaaaag
    24721 tggatgacaa gaaaagctat ctcgtcctcc agtatcttgc caccaattac ttcttcgatc
    24781 gtaggaacaa aatgagtgac tgcgcggtag attgggaaat aatttgttcg gatgttttct
    24841 ttcaatggat tcatgacttg gatatgattc ttgattctta caatcagtgc agccatgtgc
    24901 ttatagagat tttcgaataa agcttcttcc tttagatgga aaggtacgtg tgtctcgttc
    24961 tctacaaatt ggatcaattg gatcgttagt aattgagctt gtacccactc gattgaattg
    25021 gagatatctc tagcattgaa cgtctcaata gtgaaacgaa gataatttct ttctacttgc
    25081 gggacattgt ccaattgaaa ttcctcgatc atgtggtcaa taaatgcaga aaagctactg
    25141 tcttttattt ctgtattttt ccaattaatg gcagagataa attcatgttt cctgattcgc
    25201 tggatccaga tcaatgtaaa aagcaggatt tgttttttat aaatatcatc ttcttgtttc
    25261 aacaacttat tggaatagat ctcctgtatt tttttgattt ccaccaaagg aaaataatgg
    25321 agaaagattt tttgagccgg tgttaattgt gcatcttgat tcataaagaa gtcggtcatt
    25381 cccagattct gattgataaa atcatagatc atcgtacgga tagatcgttc tgaaccttta
    25441 taaaccagtc cttgctttcc ataactgacg atctctatcc cataactgac cagtgttgct
    25501 cgtaaccgtc tcatatcttc atctaatgta cttttagaga tttgaaattc ttcttctttt
    25561 ttgtttagat ggatcgggat tcggctgtaa gcaacggcta aaactagatc aaaaatacgt
    25621 tcgcgccgat tcaaaaagcc gtctttcaca tcttttattt cagtttctaa tcgagcttgc
    25681 ttttcctttt ccagcttcaa caaaaaaact ttgttgcgaa ttgtacggat ctccggaagc
    25741 tttttactgc tcaaccagat attcgcttca agaacttcct gacggatcgt ccgctgactg
    25801 acattgaact cacttgctag atgttttgct tttactggat attgggctgt catgattctg
    25861 tgaagtattt caattgttct tgttttcata gaaatgatct ccttctgatt tcaagtatat
    25921 ggttccgctt acataataaa aagtagatat ttctgccact tgaagtggaa agaaagtaag
    25981 caaagcaaac aaaaaataag ccatatgctt tttcatctcc tcaacgaaaa agacatacgc
    26041 ctattattac aaaaattttg cccatctata caaaaaagag gttgtgaaaa agctgtcctg
    26101 catcaagtaa taagaacaac taaactaaaa tagtgcttcc tatttttcgt ttggttgttc
    26161 ttattatcgc agatgcaagc ttttaaacac catttagtgg agaggttgtg acagagatgt
    26221 tcagccttga gcgataagaa gcgattttga aaaatcgctt cccatatttt acaaaatcgt
    26281 gccttattgc cgataggcta tctctggcac actgtttatt cagaaaaagg agctgcgaca
    26341 aggcttttgt cacaactccg ttcataaata tttcctgtca atccagcata atcggctgaa
    26401 taaaatcggc ttgccatctt ttttctttct cagaaaaatc aaaataccat atctgagaaa
    26461 aatactgtcg ttgtgtctta cttcctgaga tcaatcgccc atcttttcga tcgactgaat
    26521 aatcacagca tctgaaatca attcggatgg caaaactatt ttcgtggatc tgtcgataat
    26581 ttcctacttc ctgaatagtt atttttttag tatgatttct tacaccgtct gctttgtttt
    26641 tttgcagaac tttctgatgt tcggcaaaca gcttgtctgt ataacagctt ctggcttttt
    26701 caggagtttc ttcttcccaa gcggattgga tcttcatgaa tatcttttgg atttccagaa
    26761 actgtgcttt cttttcttta gccgttcctg acatgcggga caatatctcc gctttcagcc
    26821 ttttcttttg tcttttattc ttgacgatag aaaaagcaga aacccctcct actgacaaaa
    26881 cagctaatgc aagaaatccc gtttgctcat aactagaagg acctgtacct ggaccataat
    26941 acccataaga atctctgccg taagaacgac tgccaaaata actgccccca tatgaacgac
    27001 caccaaatga acctctgccc gaacttcgac cgctagatgt acctccacca tgaccacccg
    27061 cgacagcgaa tccttgggtc ggcagagcaa acaaacatag gcccactaac aaaatgattg
    27121 ctaaataatt gatttttttc attgtcttcc tcgttatctt ttattacaaa aaactatttt
    27181 tatgatagcc ttgctaaaag gaagagtcaa taaccagatg cataaactgt ttttttagca
    27241 attttgtctt atttagtcat tgagtaacat cggacaaatg tatgtgtcgg cagtccataa
    27301 ttcctctatc ttcagcatga acaagcttgc agacaagaga tctgcgcttc ctccaggaga
    27361 gatttggttt tcctgacaaa agtgcaacag tgctgtaaat ggacgatttg gtaaagcact
    27421 ttgactagca gcatgagcaa gtttttgcat ttccactaac gttttctgat cactgcgatg
    27481 caatacgcat gtgtcatcta gcgaactcat caaaactaac aacatatgca tttgtttctg
    27541 tacatatgaa tcattatcta gctccttagc tgcttggatt gcaagcctga tatgcggata
    27601 ccctaatatc gcttcttcat atgcaccatt tacgccatat ttttgcttca ccctatgacc
    27661 atgagtggtt tttttatgac gatattttgt atcaggaaaa gcagcaagct tgctgacatc
    27721 atcaagtatt ttcgataaag aaaaaggctg ttccatactg attttttgtg caatgacact
    27781 gatcaatagc cccattgccc aaatcgctcc tttatgggta ttgatgccat ttgtagcaat
    27841 cagcattgcc ttttccgctt ttcgcccaat ctcagcaatt ctttctctta actcctgatc
    27901 aatagcatga tgccatgcta tttgagccat ttcttcaaaa aaaggagtta gcgtcttagc
    27961 agacttaaga aataaagcat agtccatatc ggaatgtgca cccgcatcca gtgcatccac
    28021 taatccaggt tttggactta atgttacttc tgcgatcaat gcttcctcta ccaacgcagc
    28081 gagttgctga gtcactgctt tttttcgttc gtcatttatc atgacactca ctccttcttc
    28141 ctactattac ttgctgaaac cttttttgat agctacccaa atgatatgaa gtaaccagct
    28201 taaagcaaat gcacaacttg ccgtcattag ataagctaca agaatcatga ctcctgaagg
    28261 taactgcagc caactatctt taaacaataa taacagtact tggaaaacaa atacattggc
    28321 taaatacgta cgataagcat aaacagacaa ccatttgaac aatggcaatt ttggcgaatc
    28381 gtttacaatc attttggaag cgcctatgaa gacagcaaag atgatgacta aactgtataa
    28441 actttggatc gtattcaagt aaggtgcatt cgcaaagctc aagtctcctg gatgtgcgag
    28501 caatgaacgg acagaaaaga acgcgataac taaggcaaca ggtaagaaaa gataacgtac
    28561 tctgtccaaa tattgataga ctttttcgtg ataaacaaat gcggctaccc cataaatcat
    28621 gaaactagca acaaaccgat ctaataagta ccagctgtca tgataaggcc cagtcaatac
    28681 ttgtgtttga tagaacagat accatgcgac atataaaatc gttgaaccaa tcaatactgg
    28741 ccatacatat tttttattag caaatacttt gtaaccgagc caaacaaaaa acggcatgaa
    28801 caattggatc tgcagcatca taacggtata ccataaatga ggcgctccat cacctgtgac
    28861 atatttcagt aaaaagcttg cagtatccgt atacggagtt tgttgttgga ctgctggaaa
    28921 tagccaaaga taagccgtcg tccatagaag atacggaacg actagttcaa aaaacttttc
    28981 tttcaaaaat tcccaataag acaaatgctg acttgttttt accatattat aaacaattgc
    29041 aaaaataaac attggtgctg tgtatttaac gatgacgtaa aaggtaccaa gaaaagctgt
    29101 atttgttgcg ctgtggctgt ttgccaaagc aaagctcaat atcgtttgca tcataacagc
    29161 cgttacagct gatactttct ggaagtcact tacgttgatt ttgatttttt tgttttcaaa
    29221 catctctttc tctctctttt tctttattac caaagttctt tggttaattt aggtccttcg
    29281 actgttttca atagaatttt tttctctggc tctctcaaat actcttttag tgaaaaaccg
    29341 ccttgccgag tcactacttg ggtatcgaat tgctcaaaaa aagcaggatg cgattgaatt
    29401 acctgaattg gcaattcaat cggtgaattt gcgtacagca gcaaatcaaa atcactggtt
    29461 tcttttactg ccggaatccc agtcgataat tcaaatccta agctgccgcc gattccccat
    29521 ttacatcctg ataatatgtt tctagcagca tgaaattgct gataaacagg ataatccatg
    29581 attttctctt gtctatacga ttcttgttcg atgatgtccc acggatgaat gacttgtttg
    29641 acggcatgtt ttgagatttc tgttgaccat ctctgattgc gggcaggtcc acgtaaacca
    29701 atcggtagta tttctgcgtg atccgtcgac gcacgacgaa ctgttccgaa catcttttca
    29761 gttagaacga cccattcagg caaggtgaac ccttctattt catgtttatc aaaaaggaca
    29821 atatcatgtg cttgaatcat tttttatgca tcccattctt cgtccatttt ggcacggact
    29881 ttatttgttt cgacacgtcc gaatgttttg gctgtttcgt ttgtgtatcg atttgtcagc
    29941 atcgtgtcat ttgtattgcg gacatcttga atggcttcat tgattgcttc gatgactttg
    30001 ctgacatttt gttctgaagc agcttggtcg gtgatatctt ctaggaaatg atagatcgct
    30061 cctagttttg tataattatt gatatcgtaa gcgatagctg gaactttttc agcagctgct
    30121 tcgatttcag caaccgaacg ttttgtcaca cgtgcagcac tttctttaga catcgcttgg
    30181 acggtaattt ctttattgtt taaagcaatc aagcggcttg attgtaatcc atgtgctaaa
    30241 aatgcacctg aaatagcatt tcctgcaatc aatccaataa ctggatgtcc agcttgacgg
    30301 agttttgcat aagcagcagc actattagcc aaagccacat ggataccgat caattcttcg
    30361 ttatagccgt atgcttggct tggtacatcg atcacaacga tgattggccg tttcactttt
    30421 ttgtctttat cttcttcgta aacgtgatcc aaaatttgtc ccatgcggaa accttcaact
    30481 aaaccaactt caccgtttcg tacacggtac atcgggtttt taggatcagg tacgattgat
    30541 gtcacgatgg cttcttctat gaatttactt gatttaccat aatacacagt actaatagaa
    30601 gaagttggat ttttcatgcc agttagtgcc gcaaaccaat cgtaaccaac acttgctgca
    30661 gctggttcat ttccttctgc tacttctggt aagtcaattg attttgcttg atggttagca
    30721 attgcttcat tgtaatcgtc aatggccatt ggttttgaaa gatctaaaga atcaagtaat
    30781 gataaataaa atgctgagct ttcactgcga tgcgtgtctt ttcgttcgtc cattgctgcg
    30841 atgacagcag ctttgattgc ttctacacta tcagtcacta attcatcaat gatttttgat
    30901 tctacccgtt gacgagatcc taatgtattc caaatcaagt ctttgtcact agaatcaaat
    30961 tctcgaacgc ctgcttcttg ttcaataact tctggtccat tcaaaccgac tctcgctttt
    31021 tttgttgcga ttaaataact cattaatgct gaagtaatcg acataccgcc aaatgatcct
    31081 acacgtcctg gaatcaaacc gatgactggt acatattgtt ttagagcaat gattacattt
    31141 ccgatttctg aaatagataa cagtccgtag tttgcttctt gcaaacgaac accacctgta
    31201 tctagtacaa tgactggaaa gatctcattt ccttttttat tttcttctaa cacatgttca
    31261 agtgcagcaa cgatttttgc accggaaact tcaccgatac cgccaccttg gaattttcct
    31321 tccatagcga ttactactgc ttgtttgcca ttgatcgtgc cacggctgac aacgatcccg
    31381 tcatcacttt cagggacgat tccttgtgct ggtaaacttg gcgcgatgat ttgatcgaat
    31441 ggatcaatca attcacgtcc attgtcaagt agagcgttca ctcgttctct ggcgtttaat
    31501 tctacaaaac tatttttcat ctgaaagttc ctccattgct tgcgtcaatc gtaggtacac
    31561 aactcctggt gtagcaccga agtcattgat cacgatatct gctgaaattg gataacgatc
    31621 aaaaaatctc gttaatacat tgttccatac ttcgccgaag ccatcgcttc ctgtacaaat
    31681 ttttacagta gattctgttt tttctgttgg aaacatcaag atttccaaat ctcctgaacc
    31741 gaccactcct acatggattt tcttattgat cggctgtttc gttggatatt tataagctaa
    31801 ttgttccatg gatctctccc ctttttgtaa aacagagcat acacgttctg cttattcgtc
    31861 tttcttcttc catctgggta aatggcgaca aaaggttttt ctcgccaatt ttatgaccaa
    31921 gttctgaatt tcattggtgg ttcatacagg ccatctgacc attcgatcaa atcatcgatg
    31981 gtttgtgcag ctaataatga acgtttcgct tcactacgtt tgatgcctag atcttctggt
    32041 aatttcacga tgccggctgc acgcaagcta tctagtgatt ctttcgtgct agtcattcca
    32101 agaggtgtaa caccgccaat tgctgcaatt gctgcttgtc gttcttcttt tgtttttgct
    32161 ttgtacaaat aagcaattcc ttcttctgtc acgatatgtg tagtatcttc gctataaatc
    32221 atcactggtg cattggctaa gcctgcttgt ttttgaacag ctagtgcatc taattcttcg
    32281 acaaagactg gttttttatt tgaaccatat gtttcaacca tttgaacaac taatttttgg
    32341 cctttcccta atggatcatc attgtcgcga agtgattgcc atgctggtgt agaatgacga
    32401 cgaccgccag gattatgtcc catgttaggc gcaccgccaa aaccagataa acgtcctttt
    32461 gttacggtag aagagtttcc ttcgtaatct aattgcaagg ttgaaccgat aaacatatcg
    32521 atggcatatt gtcctgcagc ttgtgataat gttcggttac tgcgcatcgt gccgtcttta
    32581 ccgacaaaaa agacatctgg gcgagcagca atatattttt ccataccaac ttcgccacca
    32641 aaactatgga tgctttcaac ccaacctgat tcgatggctg gaatcatcgt tggatgtggg
    32701 ttcaataccc agttcttcgc gattttccct tttaacccta gtgattcacc ataagttggc
    32761 agtaacaatt cgatagctgc tgtattgaat ccaataccgt gattcaaaga ttgtacgccg
    32821 tgttctgcgt agattccttt gattgccatc attgccatta gaatttgcaa ttctgtgatg
    32881 ttttgaggat ctcgtgtgaa taaagcttct aattgataag gtttatctgc tttcacgaca
    32941 acgtctaccc agtcaccagg gatatctaca cgtggtaaag aatcgacaac ttcgttgact
    33001 tgaacgatca cgataccatc tttgaaagct gcagcttcga tgattgttgg tgtttcttcc
    33061 gtattgaaac ctgtgtacaa gttgccgtct ttatctgctt tatctgcagc tactaaaaca
    33121 acatttggaa tcaaatcgat gaataagcgt ccataaagtt ccaaataagt atggatatca
    33181 cctaatttca ttgtgccgtc ttcaatcatt tgggcgacac gcaagctttg tgcaccagca
    33241 taagagaaat cgatcttgct tgcgattcct ttttcaaaaa tattcaaatg ttctggtcgt
    33301 gagatactag acatgatcaa atgcagatca ttcacttttt ctgcatctac tttttcaaga
    33361 ctttctgaaa gaaaacttgc ttgtttttga ttgtcgcctt ctaatacaac tttgtcgccg
    33421 ggagcaatca gtttttccat tacttctacg attttttcag tttctacata cttgccattg
    33481 agcaagttgc tgattttttc caatcgctgc gctttttttt cgcgtttaga agcccagttg
    33541 cggtttggtt tttccataat tagtcatctc catttttgat atagaaacgt ggcgtcatca
    33601 ataccatgtt ggtctagatt gatcgtacgg atccctttat ctacaccaaa ttttgtatga
    33661 atcagctttg tcaaagtatt gccgggggga aattcaatcg tcagatccat tcctaattct
    33721 tttgccaagt ccatcatttg gttccactgc acagggaaag ctaggttttc caacaaatct
    33781 ttacgaatct cttctgcgtc ctgagtggct cttccatccg tattttttat atacaaacat
    33841 ctagcatcag atagatggac agactgcata tacggcttga atttcttgac cgtatcattc
    33901 atcaagagac agtgagatgg cacagggact ttcagcagct tcgctgtttg cgcttggtgc
    33961 cgtttggctt ctgccaacgt aacggataaa ccttcgatac tcccagataa cgtatgttgc
    34021 atcggacaat tttcatttga cagataaact ggatggtcag gatcaaatgt ttttgctaca
    34081 atttcctcag cttcgcttct cgtcacacct actactacgc ccattgcata accgcttgga
    34141 taagcttctt tcattaagac tgcgcggtga tagactagtt ccgctgcatt ctcaaatctc
    34201 aaagatcgac aagcaacagc tgcacaaaaa gcaccaatcg aatgtcctgc tgcaatatca
    34261 ggctgaatac cgtttttttc catttcatct aaataaaata ctgcttttgt cagtaatgct
    34321 agctggataa aaaccgtatc ttgatagttt tcttctttat caactaaccg ataccccgtc
    34381 acattttcta aacgttccat gtacatttca gggatatccg ctaacatccc taacttctga
    34441 ctgccttgtc caggaaataa gaaacctgta ctcatgagcc tcgcctcctc agcacttact
    34501 ttttccactg ctatcttagt caatttctgc tataataaaa aatgccaaaa aaagaaaaaa
    34561 ccataacaaa aaggtatgga tgggatggat aattatgaaa ttgcttcatc tgcagtattt
    34621 taaaaaagta ttcgaaacaa aaaatgttac ccaagcagcc aaagaattat ttatttctca
    34681 acctgccttg agccgtgcga tcaaacattt ggaaaacgaa ttaggtgtcc ctttgtttta
    34741 ccatagcggc agaaacattg agacgaccgt ttatgcagaa gaattttatc catatgcagt
    34801 caagacatta gacacattag aagaaggaat caaattattg tcttcggtca atgagaagat
    34861 cgttacttcc gtcatattgt atttagaggt agcctctgtt tctattccga acttggtccg
    34921 aatttttatg gaaaagcatc ctgatatcca attaacgatc atgcaacatc atttaccaga
    34981 tgatattacc gatcctgtac tgtacatcac ttcggagatg aagcctggca tgaccaatat
    35041 tcctatcatg aaagaacctg tttatgtcgc cattcctaag aaacatcctc ttgctaaaaa
    35101 agaacatctg caattagaag atatccaacg cacccccatc ttgatgctgt caaagagcaa
    35161 cgccttccgt catacactgg atgcagcact tgaagcgaaa agtatcgaat tgacgatcag
    35221 ttcgacgaca gatgatcctg cgacattacg ttctattgtc aagcaaggat tagctgttag
    35281 ctttttccca aaagtttctt ggtcttacga taaaaatgat cctttcgtct tgcgggaaat
    35341 caaagatttg cccttgacac gaacaattta tttatcttct agcttcacag aagatcatcc
    35401 tttgatcaaa atgattgcaa aaacgatgcg ggaattttat ttaggaaaat aaaaaaaaaa
    35461 gagctgggac aaaattcttg tcgcagctct tttttcgaac aaacggtgtt ccagaagtaa
    35521 tttcttagaa aataagccga aatttcccaa aaatttgaga agcaattttc gtaaattcct
    35581 tcttatttct tgaagctaaa catttctgtc acaacctctt ctctattgtc ctagacattc
    35641 attgcgttga tttaaaactt ccatcaatca aatctgctag ttcatctagc ttttctattt
    35701 tacgtagatc aatagagatc caatgttctt tattcatgtg ataggctgga aaatacccct
    35761 cttttcctcg caaaattccg ccaagttcag gatcgacctt caaatcaaga atattgatga
    35821 tcctatggtc acttgcatca tcatagattt tttcttctgg aacagacatc aggaccccaa
    35881 accatttttt gcttttttga tgtctgaata cgcaataatc aggaaatttt ttaaaaatat
    35941 attctggaga caaattgtat gtttcattga tgtgatcgat tagttctttt ttcgttagca
    36001 tctgcttcca tctctttctt attgattcgc tttattcaaa gataccacgt aagaatagaa
    36061 aaatgaatac aagtagttgc taatcaaata cttcgatatt tttctttttt atcaactctt
    36121 aaatgttcaa tccgcctgtg ttggtcttgc taccatctac tattaaaatg tttattattg
    36181 tttgcccacc tattttttga ttttagtggt atcatttttc aagttcatga tgaaattctt
    36241 atcatcagta aatgctccag aaaaataaac aatgattgaa aaatcagttg aaacttgacc
    36301 accgtttatt ttctccgtat tcttagaata gaagggagtc tgattgatgt attcactaat
    36361 gaaaaaaatc attaccgaaa aagatattcg acgtcatgtt tctttagtag aacaattgct
    36421 gaatcataca aagataaccg taaatgaatt agctgaaatc atagggacaa ccgaacgaac
    36481 gattttttct gaccttcaat ctattcgctc tcagctgccc gaaggctggg acatcatcag
    36541 cgatcaagca ggaatcagtt tgcaaaacca gcaaaattta ttaacaaatg acctttggga
    36601 gatttttttc aaacaatcag tcagtgtcga actgctgaaa aatctgcttt ttacaaaaaa
    36661 agttgctgtc cctgattttc tagccgacca tgggctttct tatgggacat tgaaaagaca
    36721 tgtgactaaa atcaatcagc gtttagcaag ttacgacttg cagatcgatt tgacgaaata
    36781 tacagcttgt attcttggaa aagagcgtgc gattcgtact ttttatcatc gattgctgat
    36841 tcctttcact cataacaact attttttcga ggattattcg atccatgaat cccattattt
    36901 tcaattttta agaaacctca gtcaaactga actagcggtt gaaacagaag agattttcgg
    36961 aacatgttgg ttctttatca atacgatccg tataaaagcg aattgccgat tagattcttc
    37021 tatccatacc aacagcacac tctcctccct gtatgactct gcattgaaaa aattgtattt
    37081 aacggaaggt atttatctta aagacactga gctttctttt gcttctttct gctttctgga
    37141 aagttggaat tacaacaata actatggaca agaaaccgct cgctgcctgc atcattctcc
    37201 atttttagag gtcctcgaaa cgtttgtaga ggaactagct tcagagctgt ctcttgatca
    37261 actaaaaaaa acatctctga ctgataactt ggctctcctc atattaaaat atcatgaatc
    37321 gccgatattg attgaagcat tggaccgtca ataccatcgt tttttagaaa catatgatca
    37381 gcaatatgca cacttgtatc aacagaaaga gaatttactg aacgatctaa aaaaagaaat
    37441 agaaatcaat gaaccaaatt actttctcca actcttgtct ctactgatcc aacaaacgat
    37501 tttttctatc aaaccgaatt tgttcaatgt ctattttatt ttccaaggag aaccttcatg
    37561 gaaggcgttc ttgcagcagg aattaaaaga ttacttagga aaacgtgtct gcttcttacc
    37621 tatcgaacta accgatcttt ccactatttc ttttgaaaaa acagatatct tgatttccaa
    37681 tttccctcta gattctctag atatccctat tgtatacatt tcttctattc caacgaaaaa
    37741 tgagctcaat cgtttgactg aactcacttt caaaactttt ttatagtaac ggctagcgac
    37801 tgtattctct agctgttttt tcttttttgc gaaacagttt gttcatgatc cctttttcta
    37861 tttctacaac taagaataga agaatcacat ttgctccaat caataactgt tgtgtcatcg
    37921 ttaaacttac tgtaccgatc agctgctggc cgactggtag aaaaataacc atcccttgca
    37981 ataaagcaag gatacctaat gaaagaaaaa gcgctttgtt ttgcaaaaga ccttggttca
    38041 atgacggctt ggacaactct ctgcaattga tcatgtaaac agcttgagct aagacgatat
    38101 tttgcagaag aatggtttgc tgtaagcttt gcccttcaaa ctgcatagcc aataaatagg
    38161 caggaaccat gatcagtagg gagacataga tgatccgaaa caaatgataa ccagatagga
    38221 ttccttgctt tggatctctt ggtgggcgct tcatgatttc tggatctggc gactcgaacc
    38281 ctagtgcata agataaagtg attgtcgtta ccatattgac ccataaaatc tgtactgggg
    38341 acaaaggtag cgggcgattc agcataagtg cccaaatcaa gatcaatcct tgagctaatg
    38401 cagttggcaa gaagaaattg atggtttttt gcaaattatc caaaatccgc cggccctctt
    38461 ttacggcttt tgcaatcgta tggaaattat catctcctaa aaccatatca gctgcctgtt
    38521 tgcttacttc actacctttg attcccatag caattcctac atcggatctt ttcaaagcag
    38581 gtgcatcatt tacgccgtct ccggtcattc cgacgatttc tccatttttc tgcaaagctt
    38641 taacaattcg cagtttatgt tctggtgtcg ttcgagcaaa gacatcgact attggcactt
    38701 gctgaatcaa ctcttcatca gacataagat ccaaatcgat cccttctaaa actttggcag
    38761 tatgttttaa gccaatttgt tccgcaatcg cttttgctgt gtctttgtga tcccctgtaa
    38821 tcattttcac tgagatccct gcttgttggc attcctttac agctttgact gcactttctt
    38881 ttggcggatc gatcaatccg gcgatccctg caaaagttaa ttgggttaaa gtctcatgcg
    38941 tgagttcctg tttcgaatct acttttttat aagcaaatcc taaaacacgc tgaccttttc
    39001 tcgcaaattc tgcagctgtc tgactccatg cttcttgttc tgcagaagaa agagttgatt
    39061 tagcaaataa tacttctggt gcacctttga cgtaaatcac cgctcctttt tcctccgcat
    39121 gcatcgttgc catatatttg tattcagaac tgaacggaat ctttgcttct atttttttca
    39181 aaggtagatt ggctgtttct gtgtgtcgaa tcagcgcaac ttctgtcgga ttgccactta
    39241 aattttctgt tttctgttct tcttttgttt ttaattcctg acagtttttc ataatggaca
    39301 acacgcaggc ttcttctttt gcagctacat ccacaactgt catttcattt tttgtcaatg
    39361 ttcctgtctt atctgaacag atcactgtca tagaacctaa tgtttctacc gaaggaagtc
    39421 ctttgatgat cgccttttct tttgccattt catgtactcc aacggataga atcatggtca
    39481 aaacagctgg caatccttcg ggtaccattg acaccaccaa agcaatcgta gccgaaagaa
    39541 ggatatgcca atccatacca tatcgaaatg tcgtaaaaat aactaagaat aggctcaaac
    39601 aaagaatgcc ttgaaaaatt tgtttgttta atcggttgat ttttttaatc agaggcgtcg
    39661 tttgttgttc gaccgattgc aatgcttgat tgattttacc gatttctgtc gtgtttcctg
    39721 tttctacaac gacaccgact gcagaaccag attgtacaag cgttcctgaa aaagccatat
    39781 ttttctggtc gccgatcgat tcttcttctg tcaaaacggc atgacttttt tcagatgctt
    39841 ctgattctcc cgtcaaaatc gattcttcca ctaataaatc atgtgcttcg attaatcgga
    39901 tatctgctgg aacgacatct ccggcacgta agacaatgac atctcctttt accagttctt
    39961 tcgcagggat tttttcttgc atcccgtttg atagtactat cgcttcttgt cccatcagct
    40021 gcttcaaacc attcaatgat tcttccgctt ttctttcttg ccagtaacta acgagtccat
    40081 tgacgatcac tacgaataaa ataatacttc cttcaatata ttcactagaa gcaaatttca
    40141 aaaaagaggc aatgatcaac acgatcatca gcagatcagt aaaatgcttt gctatttttt
    40201 tccatcgttt tgtctgctgc cttgctttta tttcattata tccttgatca gtcaggcgtt
    40261 tctgtcgttc ttcttgcgac aagccttctg ttgatgtttg atagtttttc aatacatctt
    40321 ctaccgtcaa tctgtaccag ttcatcctat cccctcctgt ttaattgata ctgttattat
    40381 acgcagggaa gttttttc
//
LOCUS       DACDMM010000007        40144 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDMM010000007 DACDMM010000000
VERSION     DACDMM010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.007"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..560
                     /locus_tag="IWD16_000272"
     CDS             <1..560
                     /locus_tag="IWD16_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9087359.1"
                     /translation="KNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTKH
                     IFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAH
                     KVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSEV
                     GYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            760..1074
                     /locus_tag="IWD16_000273"
     CDS             760..1074
                     /locus_tag="IWD16_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9087360.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1095..1472
                     /locus_tag="IWD16_000274"
     CDS             1095..1472
                     /locus_tag="IWD16_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9087361.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1482..2255
                     /locus_tag="IWD16_000275"
     CDS             1482..2255
                     /locus_tag="IWD16_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087362.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2277..3680
                     /locus_tag="IWD16_000276"
     CDS             2277..3680
                     /locus_tag="IWD16_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9087363.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3862..5046
                     /locus_tag="IWD16_000277"
     CDS             3862..5046
                     /locus_tag="IWD16_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9087364.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5043..5333
                     /locus_tag="IWD16_000278"
     CDS             5043..5333
                     /locus_tag="IWD16_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087365.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5330..5551
                     /locus_tag="IWD16_000279"
     CDS             5330..5551
                     /locus_tag="IWD16_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087366.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5593..6372
                     /locus_tag="IWD16_000280"
     CDS             5593..6372
                     /locus_tag="IWD16_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP9087367.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6439..6933
                     /locus_tag="IWD16_000281"
     CDS             6439..6933
                     /locus_tag="IWD16_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9087368.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6989..7627
                     /locus_tag="IWD16_000282"
     CDS             6989..7627
                     /locus_tag="IWD16_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087369.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7699..8094
                     /locus_tag="IWD16_000283"
     CDS             7699..8094
                     /locus_tag="IWD16_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9087370.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8078..10531
                     /locus_tag="IWD16_000284"
     CDS             8078..10531
                     /locus_tag="IWD16_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9087371.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10528..12555
                     /locus_tag="IWD16_000285"
     CDS             10528..12555
                     /locus_tag="IWD16_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087372.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12552..13574
                     /locus_tag="IWD16_000286"
     CDS             12552..13574
                     /locus_tag="IWD16_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9087373.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13591..14520
                     /locus_tag="IWD16_000287"
     CDS             13591..14520
                     /locus_tag="IWD16_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9087374.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14765..14881
                     /locus_tag="IWD16_000288"
     CDS             14765..14881
                     /locus_tag="IWD16_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9087375.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14897..15700
                     /locus_tag="IWD16_000289"
     CDS             14897..15700
                     /locus_tag="IWD16_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9087376.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15875..16171
                     /locus_tag="IWD16_000290"
     CDS             15875..16171
                     /locus_tag="IWD16_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087377.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16178..18130
                     /locus_tag="IWD16_000291"
     CDS             16178..18130
                     /locus_tag="IWD16_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9087378.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18202..18699)
                     /gene="dfrG"
                     /locus_tag="IWD16_000292"
     CDS             complement(18202..18699)
                     /gene="dfrG"
                     /locus_tag="IWD16_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9087379.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            19061..20101
                     /locus_tag="IWD16_000293"
     CDS             19061..20101
                     /locus_tag="IWD16_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9087380.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            20199..20387
                     /locus_tag="IWD16_000294"
     CDS             20199..20387
                     /locus_tag="IWD16_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9087381.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20445..20798)
                     /locus_tag="IWD16_000295"
     CDS             complement(20445..20798)
                     /locus_tag="IWD16_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9087382.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21329..21751
                     /locus_tag="IWD16_000296"
     CDS             21329..21751
                     /locus_tag="IWD16_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9087383.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21748..21978
                     /locus_tag="IWD16_000297"
     CDS             21748..21978
                     /locus_tag="IWD16_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9087384.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22476..22676
                     /locus_tag="IWD16_000298"
     CDS             22476..22676
                     /locus_tag="IWD16_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9087385.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22703..23896
                     /locus_tag="IWD16_000299"
     CDS             22703..23896
                     /locus_tag="IWD16_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9087386.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23964..25529)
                     /gene="guaA"
                     /locus_tag="IWD16_000300"
     CDS             complement(23964..25529)
                     /gene="guaA"
                     /locus_tag="IWD16_000300"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9087387.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25588..26511)
                     /locus_tag="IWD16_000301"
     CDS             complement(25588..26511)
                     /locus_tag="IWD16_000301"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP9087388.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26707..27312
                     /locus_tag="IWD16_000302"
     CDS             26707..27312
                     /locus_tag="IWD16_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9087389.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27620..28636
                     /locus_tag="IWD16_000303"
     CDS             27620..28636
                     /locus_tag="IWD16_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9087390.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28777..30078
                     /locus_tag="IWD16_000304"
     CDS             28777..30078
                     /locus_tag="IWD16_000304"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP9087391.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30124..30786
                     /gene="deoC"
                     /locus_tag="IWD16_000305"
     CDS             30124..30786
                     /gene="deoC"
                     /locus_tag="IWD16_000305"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP9087392.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30807..31205
                     /locus_tag="IWD16_000306"
     CDS             30807..31205
                     /locus_tag="IWD16_000306"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP9087393.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31271..32353
                     /locus_tag="IWD16_000307"
     CDS             31271..32353
                     /locus_tag="IWD16_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9087394.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32565..34127
                     /locus_tag="IWD16_000308"
     CDS             32565..34127
                     /locus_tag="IWD16_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9087395.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34120..35253
                     /locus_tag="IWD16_000309"
     CDS             34120..35253
                     /locus_tag="IWD16_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9087396.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35253..36209
                     /locus_tag="IWD16_000310"
     CDS             35253..36209
                     /locus_tag="IWD16_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9087397.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36308..37474
                     /gene="deoB"
                     /locus_tag="IWD16_000311"
     CDS             36308..37474
                     /gene="deoB"
                     /locus_tag="IWD16_000311"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP9087398.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37498..38316
                     /locus_tag="IWD16_000312"
     CDS             37498..38316
                     /locus_tag="IWD16_000312"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9087399.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38333..39040
                     /gene="deoD"
                     /locus_tag="IWD16_000313"
     CDS             38333..39040
                     /gene="deoD"
                     /locus_tag="IWD16_000313"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9087400.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(39338..40024)
                     /gene="gpmA"
                     /locus_tag="IWD16_000314"
     CDS             complement(39338..40024)
                     /gene="gpmA"
                     /locus_tag="IWD16_000314"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9087401.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
BASE COUNT        13246 a         6938 c         8200 g        11760 t
ORIGIN      
        1 taaaaaatga atttggaata gaagttaaag attattttat tgacttagat ttattacagc
       61 taataaacag tgtgaaaaga atgtatagtg gagacgacaa cggtgttgat ttatacaaat
      121 ttgttaccaa acatatattt gttaatctta atattgacga gaaaaattac ccgtctattt
      181 ttaagcacta tctatttttt attaacgaag tagaagatcg gataaaaaat aattcattat
      241 ctagtagtgt atctagtttt aagaagatgt ttaaatctcg agatggtatc acacttacta
      301 cagcacataa agttaaagga aatgagtatg atactgttat cgctattaat cttctaaacg
      361 ggaaaattcc tcattggaat gaaattttta atgtttctga taaaggaatt agttcagcga
      421 gaaaactact atacgtaatt tgttcaagag caaaaaataa tttatttctt ttctcagagg
      481 tgggttattt tacgagtaaa ggagacttgc tttatcccac tcaattatta gataaaatag
      541 aatttgatta taatatttaa aatacctagg tagtcgttgt tttaaatata gtaggaatcg
      601 gacgctcagt acggagcgtc tattttttta cccaattttg aaaggaagtg aacttatgaa
      661 aataaaaaat caagaatcaa aaggtcgctc tccccccttt aaaaccatca cacaaataag
      721 attcattcaa acataaaaac agaaaggata ggtaaaaata tggaacttaa atatgtcatt
      781 cccaatatgg aaaaaacatt tggacaatta gaatatgctg gtgaagggaa cattgaacaa
      841 cgtcgaataa atggcagacc aaccattctc tctcgtagtt acaatttata ctcaacgatt
      901 caacgagcag atgatattgt tgtcattctt ccctccgaag ctggcgagaa acattttgaa
      961 tccgacgaaa aagtcaaact catcaaccca catattaccg cagaaggtta taaaattggc
     1021 aatcgaggat ttaccaatta cattatgcac gcagacgata tggtgaaagc ataagaaagg
     1081 aaaggtgaaa aaatatgaga ttagcagaag gtattgtgat tgaaaaagac cagacatttg
     1141 gcacattgaa attttccgcc ttgcgtcgtg aggttcgtat ccagaatgaa gatggtacgg
     1201 tttccgaaga aattaaagaa cgtacctatg acttaaaatc aaaaggacaa ggacgcatga
     1261 tacaagtcag tatccccgct tctgttcctt tgaaagaatt tgactacaac gcagaagtgg
     1321 aattaatcaa tcctgtggca gataccgtag cgacggctac ttttcgaggg gcagacgttg
     1381 attggtacgt gaaagcagac gatcttgtat taaaaggaca gacaaaacct catcagaaat
     1441 ataaaaaaga agcgtccgaa agtgaaaaat aggtgttgct tatgacaaac acaccattga
     1501 ttttgaaaga aattccgaag gatgaagcca tttcttttat tcggcaatac cattattcaa
     1561 agatactccc tagattgtgt aaatactttc tagggatttt ttcagaagaa aaattattag
     1621 gcgttgtgga attaggctgg ggtacgcaac ccctacaaac catacgcaaa ctctttcccg
     1681 acagttccct tcaaaccaca gattatttag aaattggaaa gatgtgtttt cttccagaaa
     1741 tgaatcaaac caattatttt ggcagtcaag ctctttcagc tctaattaag tggttgaaag
     1801 aacatacgga ctgtcatttt ttatatacac tggcagatgg aattgaggga aaatgtggct
     1861 atgtctatca agcgtccaac ttcttttact gcgggtattt caagacaagt gtctatcgtg
     1921 ataagcagtc atgggaaaag atacaccctc gaagtgctcg ccttttatta gaggaaaatg
     1981 ctcgctttga acaggtagag aaaaagcatt ggctttccca ggcattttgc gaatacaaag
     2041 ggattgagaa aatcaatgga cgcatgtttc gctatctcta tccgttgaca aaagaagcga
     2101 aaaaactgtt aggacacact ctctatcgac gtcattatta tccgaaagaa aaaaatttac
     2161 gctttgaaaa gcgaatcgct tatcagaaat atgaagccat ttctcaacca acttttgata
     2221 aacaggctcg tatctacaat acacaattat tttgataggt ggaaaggagg atttttatgc
     2281 cattgcttca tgacagagga gagcgaatca aagccaacga taagcacttg gtctatcatt
     2341 ttgtactaat ttcattgctg gtagtttttc tagtcgtcct actccctttt cattggagag
     2401 cgttacaatc ggtgaactgg caagcagttc agttagatac atttttctat caacttcata
     2461 cgccatactt ttggagcagt attctcactg ctctttttgt atgcggactt atcagctggc
     2521 tttattatca ctatcgtata gattctataa aacagttaga gcaccgtcaa aagttagctc
     2581 gtatgatttt agaaaatcat tggtatgaag ccgaagaaac tcaatcagag ggattcttta
     2641 aagatttacc cagcaagaaa accaaacaac gaatccgcca ttttcctaaa atatattatc
     2701 gaatgaaaga tggactaatc catattcatg tagaaatcac cttagggaaa tatcaagatc
     2761 agcttttgca cctagaaaac aaactagaaa caggattgta ttgtgaatta gtggaaaaaa
     2821 cgttgaaaga ttcttatgta gagtatgtcc tactttatga tactattggc aatcgaattg
     2881 gcattgaaga cgtggtagct caagatggac gtgtcaaatt gatggaatcg gtctattggg
     2941 agttcgattc tctccctcac atgttaattg ctgggggcac tggtagtgga aaatcctatt
     3001 ttattctcac attgattgaa tctctcctgc atacaaatgc aaagctttat attcttgacc
     3061 ctaaaaatgc tgatttggca gacttagggg cggtcatgaa taatgtctat taccgtaaag
     3121 aagatatgtt ggcatgtatc aatcaatttt acgaagatat gattgatcgc agtgaaacca
     3181 tgaaacaaca ccctaactat aaaacaggag aaaattatgc ttatcttggt ttgtctgcca
     3241 attttcttat ttttgatgaa tatgtggcaa tgatggatat gctaggtcgt gaaagtacca
     3301 ccgttattca taaactcaaa caaattgtaa tgttaggacg acaagcaggc ttctttttaa
     3361 ttcttgcctg tcaacgtcca gacgcaaaat attttagtga tgggattcgg gacaacttta
     3421 acttccgtgt ggctttgggt cgtatgtctg aaatgggctt tggtatgatg ttcggaagcg
     3481 atacacaaaa acaatttttc ttaaaaccca tcaagggaag aggttacgtg gatacaggaa
     3541 aaagtgtgat tagtgagttt tatacacccc ttgtaccaaa aaggtacgat ttcttaggag
     3601 aaataggaaa agtcattcaa aaaaagcaat ctgagcctgt accgcacgaa gtgaaaggta
     3661 caggttcaga cttgctttga ttggtgtggc gttagccacg ccaatccttt agccccctct
     3721 atctaacagg ggggcacaaa tacaactgaa aacaggcaat tctcatcaac caatgttgat
     3781 ggtacaaagg attgcggaaa atatgaaagg tgtaaactca agggtcatag tttacatctt
     3841 ttttagattg gaggtttgcg aatggaaaac gaaaaaattt ggattaagga attgaaagaa
     3901 aagcgactgg cttatggcgt ttctcaaaat aaactggcgg ttgcttctca tattaccaga
     3961 ccttacctat cggatattga aacaggcaag gcagtcccta ctcaacaggt gaaagaagat
     4021 ttactgaacg ctttggaacg cttcaatccc gataacccat tggagatgtt attcgactat
     4081 gtacggattc gattccctac caatgatgtg gctcaaatta tcggtgaggt tttacgtttg
     4141 aatatggatt atatgctaca cgaagacttt ggctattatt cttatcccga acactatcgt
     4201 ttcggtgata ttgttgtcct agtctctcat gatgtaagta aaggtgtttt attagaactg
     4261 aaaggaaaag gctgtcggca atttgaaaac tttctattgg ctcaacatcg gagctggtat
     4321 gatttctttc aagattgcat ggaacataaa ggtattttca aacgcttgga tttagctatc
     4381 aacgataaaa cagggatttt gaatattcca gatttggcaa agaaatgtaa acaggaagaa
     4441 tgcatttccg tctttcgaag ctttaaaaat tatcgcagtg gtgaattggt tcaccgtgat
     4501 gaaaaaccat acatgggaaa cacactttat attggctcat taaaaagtga agtctatttc
     4561 tgtatatatg agaaagatta tgagcaatac gtgaaaaatg atgttccctt agaagacgca
     4621 gaagtaaaga atcggtttga aattcggtta aaaaatgacc gagccaccca agccatgaaa
     4681 gatttattgg cacatcaaca ggcggaaaaa acagcctttg aaattatcaa tcgctatatc
     4741 cgctttgcag ataaagacga cactaaacgt cggagtgatt ggaaaactaa tgaacgttgg
     4801 gaatggttta tcggtaaaaa tcgtggagca ttacgcctaa ccacacagcc agagccttat
     4861 tcttttgaac gaacattgaa ctggttacat catcaagtcg cccctacatt aaaaatcgca
     4921 agcattttag atgttctcaa cggtacaacc attatctcca ccatgattca ggaagccaaa
     4981 ctgacagaaa aacatgagaa attaattgaa caacaacatc tagccatgga ggatttgatt
     5041 acatgagcct tttaacaaaa gaattaaaga agttagggtt tcagtgtggc attgagtttc
     5101 aagcgtatat acaaaataca gggaaatata cgtctctcat tattgaggga aaacggcaag
     5161 ctggtgatac catctatacc tatgattttt acaaggtgag attttataat aattatacca
     5221 atcgagtaac cgtctatgga gagcatttaa ccccttttca attattaagg cgtgtaaaaa
     5281 gctatatcta ttatcgagaa aaatatctta aggaaaggag gacaatcaca tgaattttgg
     5341 gcagaatttg tacaactggt tcttggataa tgcacagtca ttggtattgt tagcgattgt
     5401 tgtcattgga ctgtttttag gttttaaacg agaattttca aaactcattg gcttcttgat
     5461 tattgctcta atcgccgtcg gacttgtgtt caatgcagct ggcgtgaaag atgtgttatt
     5521 aaacttattt aaccgaatca ttggtgctta acagatattt ttcatgataa aataggaagt
     5581 atatattttg ttatgaaagg atttatgaat atgtttacaa atgatcaatt taaatctctt
     5641 ttaattaaaa cattagaaag aaagagctct tcaaattatt atgaaggagg taacgagatt
     5701 gtttctaaaa tgaaaatatc agaggtaact ttagatgaga ctgacgattt tacttattat
     5761 aaaggatata aaaagggctg gaatacgctt tgcacatatc ttaaaataag agttcctttt
     5821 gatgatttag acttttttga aagtcataaa gatttgatta cccagacagc aagctccata
     5881 tatgataaac aaggtgataa tgtattagtg gatactattt tagtaccact tccagagaat
     5941 tacgaagtaa ttaactttag tcagttgaaa attagtgatg tggtttcaca agctattgaa
     6001 gacgctgagt cttttatgag taatggtgaa tatcaacgtg cctttgacag agttcacact
     6061 gcttttcatg gatatttaat tgaaatacta aaaaaatatg aaattacagt tcctagagac
     6121 gaaaatctat ctaaattata tagtaggata caacaattaa ttgaaaaaga aattcaacct
     6181 actgaacttg ctgatatagt gaagacaacg ataaggtcat caaatggaat gattagctct
     6241 cttaatgaag caagaaatag gcattcactt gcacacccta atacaaatat tattggaaag
     6301 agagaagcaa aactaataat aggtatttca agtacagtta cagattatat tagcggatac
     6361 cttgataagt agcgtcatta actgattgct acttttttta tgactatttt tataaaaagg
     6421 atgtgataag aattggaaat gaaagtctat gtggcaaatt taggacggta taatgaaggt
     6481 gaattagtcg gagcatggtt tactccacca attgatgagg aagaaatggc agaaagaatc
     6541 ggtttaaatg aggactacga agaatatgca atacatgatt ttgagcttcc ttttgatgtg
     6601 gacgaataca cgccgattag tgaaatcaac cgtctatgtg aagccattca agaaatcgaa
     6661 ggtacaccta tttataatga actcaaagaa attcaaggca tgtggtttag tagcttggaa
     6721 gaattactgg aaaacaaaga ggatattcat tgttatagtg attgtgattc gatggaagat
     6781 gtcgcccgtt attacgtgga agaaacagga cagcttggtg aagtcccttc caatttacaa
     6841 aattatattg actaccaatc tttgggacgt gatatggaaa tagagggtaa ttacctagtt
     6901 acctctcatg gggtatttga atattgtcag taagtttaac aatccgtgtt ataattaaat
     6961 taataaatag cacggagagg tataaattat ggtaaatgta tttgaaaatc ttaaactatt
     7021 aaaactagac atgaaagata aaggctgggt gatagattcc ttttactttc gttacaaaca
     7081 gcaaaattat attgtacttg ttaagctatt tgaaaaagag gaaaaagttc cagaatatgc
     7141 tctgttaaaa ctagaatttt taaaggaaaa cgatttttca gatatgctag ctgtctatgc
     7201 taattcggta aaattatata cagacacgaa aacaataaga gaatattttg gtatcgaata
     7261 cagtagtaac ttgggagatg ttttatttca atttagccaa actcttgctc gatttattcc
     7321 gacagaagtt agtgagaaga aaaatgaaga ccaaaaagaa gccatgtgtt tctcattaag
     7381 tcaaagtgat tcagaagatc ccagaaaaaa atattgtttt tcagttagaa gaaatccttt
     7441 aaagggaaat ggtgagctgg gaaaacgaag tccttttaat gacaataaaa cgagattgta
     7501 tagacccagc ttatatgaac gattagggtc agataccaat ttaagtttca gatattcaat
     7561 gaatccagat gatgaagaaa ctgatgaagg tattattgca aaatggacaa aaaataaaat
     7621 tgaatagtta aagcattgaa agagcaatca cctattttga ttgttctttt tctattttta
     7681 cggaaaggac gtgaagacat gaaaaaaata agaagttaca ccagtatttg gtcagtagaa
     7741 aaagtgattt atgcgattaa tgatttacaa ctccctttcc caattacctt tacgcaaatg
     7801 acatggttta tcgtttcttt gtttgtcgtc atactctttg gcagtttacc accactttct
     7861 ctgattgacg gagcactact caaatactta gggattccca ttgttctcac tgggttcatg
     7921 tgccaaaaga cctttgacgg aaagaaaccc tatggatttc taaagtctat cattacctat
     7981 gctctacgcc ctaaagtaac ctacgctggc aaacaaatac gattaaaaaa agaaacaatg
     8041 aatgaatcga ttacgattgt aaggagtgaa caactagatg gcatatccga ttaaatatat
     8101 ggaagataat ctcgtattta atcatgatgg cgaagtattc gcttattatg aattaattcc
     8161 ctataattac agttttttaa gttccgatga aaaaattcaa gttcatgata atttctgtca
     8221 attgattgcc caaaatcgtg atggcaagat tcatgctctg caactagcaa ccgaatcaag
     8281 tattcgagcc acacaggaac aatcgaaaaa agaaatcact ggacgattaa aagagatagc
     8341 ttacgaaaaa atagatgaac agaccgaagc gttaatttct atgattggtg acaatcaagt
     8401 agactatcgc ttttttattg gttttaaact ctacctcaat gaacaggaag tatccgtaaa
     8461 aaatgttggg aaagaaattc aaaaagcatt tgcggaattc tttcatggtg tgaatcacaa
     8521 attaatgggc gattttgttt ctatgcctat ggcggaaatc gaacgctttt ctaagatgga
     8581 acagttgtta gaaagtaaat tatccagacg ctttcagatt cgtaaattgg agaaagatga
     8641 ttttggttat attattgaac atctttacgg gcaaacaggt gtcccttttg atgactacga
     8701 ctaccattta ccgaaacgat acgaagataa aaaagtgttg gtaaagaaat acgatattct
     8761 caaacccact cgctgtttaa tcgaagaaaa tcaacgctat ttacggattg aacaggaaga
     8821 taccactact tatgtggcgt attttacaat taatagtatt gttggtgaat tggatttccc
     8881 taatagtgaa attttttatt accagcaaca gcagtttact ttccccgttg ataccagtat
     8941 gaatgttgaa attgtcgcca ataaaaaagc cttatctacc gttcgcaata aaaagaaaga
     9001 actgaaagat ttagatagcc acgcatggga aagcgacaac gaaaccagta ctaatgtggt
     9061 ggacgcttta gaatctgtcg atgaattaga aagtaattta gaccaaacca aagaatctat
     9121 gtacaaacta agctatgtgg tacgggtatc agcaaagaat ttggacgagc tgaaacaacg
     9181 ttgtgatgaa gtaaaagatt tttatgatga tctgtctatc aaattagtac gaccttttgg
     9241 cgatatgcta ggcttgcata atgaatttat tccctcaagc aagcgatata tcaatgacta
     9301 tattcaatat gtcacgtcgg attttttggc tggtcttggt ttcggtgcta cacaaatgat
     9361 tggtgaaaat tctggtattt acgtcggtta caatctcgat acaggcagaa atatttatct
     9421 ccaacctagc cttgcaagtc aaggaattaa aggctctgta acaaatgcat tagcgtccgc
     9481 ttttattggt tcattaggtg gcggaaaatc attctctaac aatatgctgg tctattattc
     9541 agtactcttt ggcggacagg caaccattgt tgacccaaaa gctgaacgtg gtgaatggaa
     9601 agaaacctta cctgatattg cccatgaaat caatattgtt aatctcacga gtgagaacga
     9661 caataaaggg ttacttgacc cttatgtgat tttaaaactc ccgaaagatt cagaaagcct
     9721 tgccattgat attctcacgt tcttaacagg tatttccagc cgagatagcg agaagtttcc
     9781 tgtcctgcgt aaagctattc ggagtgtaac tcaaagcaaa caacgagggt tgttattggt
     9841 gattgatgaa ttgcgaaaag aagatacgcc catcagcaat agtattgctg accatatcga
     9901 aagctttgtc gattatgatt ttgcccatct gttattcagt gatggcagtg tcacccaatc
     9961 cattagcttg gaaaaacaac tcaatattat tcaagtggca gatttggtct tacccgattc
    10021 caatagctct tttgaagaat acactaccat ggaattatta agtgtggcaa tgctgattgt
    10081 cattagtacc tttgctttag actttatcca ctctgaccgt tccattttta aaattgttga
    10141 ccttgatgag gcttggagtt ttttacaagt ggcacaagga aaagctctat ctatgcgatt
    10201 ggttcgagca ggacgtgcca tgaatgcagg ggtctatttc gttactcaaa acgcggacga
    10261 tttactagat gaaaagatga agaataatat tggcttgaaa tttgcctttc gttccactga
    10321 cctagtggaa atcaaaaaga ccttagaatt ttttggtgtg gacaaggaag atgaaaacaa
    10381 tcagaaacgc ttacgtgaat tagaaaatgg acaatgttta gtttcagaca tctatggtcg
    10441 tgtaggggtt atgcagtttc acccgatttt tgaagagttg cttcatgcct ttgataccag
    10501 accgcctgtg cgaaatgagg tggaagaatg acacaaaaac aaaagaaatt tcttcactat
    10561 agttggataa cgctcctttg tgtgggcgtt attttacttt tattaggcac gctgggcaat
    10621 gcagtacaag cgacaggctt agttgatgaa accattgata caagcaatga atactccaag
    10681 tatggtttga atcaatatca gcttgattat tatgtggaca atagctgggg ctggcttcca
    10741 tggaattgga gtgatggcat aggacaatcg gtgatgtatg gattgtatgc cattactaat
    10801 tttatttgga caatttctct ttatctatcg aacgccacag gttatttggt gaaagaagcc
    10861 tatgccttag atttcattag tcaaacatcc caagcaattg gcgaaaacat gcagacgatt
    10921 gcaggcatta caccgaatgg cttttccagt tcagggtttt atgtcggttt cctccttctt
    10981 tttattctca ttgttggaat ctatgttgcc tatacaggat tggttaagcg agaaacgacg
    11041 aaagtgatac gagctgtttt aaactttgtc gtggtgttta ttctatcggc ttcctttatt
    11101 gcgtatgcac ccgattatat tgcgaaaatc aatgattttt ccaaagatgt cagtaccgcc
    11161 agtctgaata ttgggacaaa aattgttctt cctaacacgg atagtcaagg gaaagacagt
    11221 gtggacatga tacgtgacag cttattctct gttcaagtaa agcaaccatg gctattgctt
    11281 caatatggct ccactgatgt agaagcgtta ggggaagaac gtgtggaaag tttattgtct
    11341 accagtccag atacaaacaa tggtgaagat agagaaaatg tagtgattga agaaattgaa
    11401 gatcatgaca atacctattt aaccctaccg aagacgattt ccagattagg aacagtcttc
    11461 tttttgtttg tattcaatat tggtatctcg atttttgttt tccttctaac aggaatcatg
    11521 attttttcgc aagtgctatt tattattttc gctatgtttt tgccaatcag tttcctatta
    11581 agtatgttgc cgagttttga tggcatgggc aggcatgcca ttaccaagtt atttaatgtg
    11641 attatgacaa gggctggtat taccttgatt attaccgttg cctttagtat ttccactatg
    11701 ctttattcgc tgtcagcaag ttctcccttt ttcatgatta tgtttttaca aattgtgacc
    11761 tttgcaggga tttattttaa actgggcgat ttaatgagcc tattcacttt acaaagcaac
    11821 gactctcaaa acatgggcag acaagtgttt cgcagacctc gtcaaatgat gaatcgacaa
    11881 accagaagat ttcaacgcag tatgagtaag atttttcgtg gaaaagcagg gtcaacgaag
    11941 caaaagacaa tgacaacaaa gcagaatcaa accaataaag ctaaccatac tcgtcaaaat
    12001 gaacggacta caccaaagaa agaaacagca ctttccaatg tgaaaaagca acaaggcggt
    12061 atcgggcaac gattaggtga aaagacagcc catgtattgg acacgaaaaa tcgtctagtc
    12121 aataaaagaa agcaggcaaa acaacagata caggcgacac caacccatgt caaatatgcc
    12181 cttcatcaag gaaaagagaa tgtcactcat aatgtaacag actttaaaca atctatggca
    12241 gataccaaag ctcaacgcaa acaacagcgt atgaaccagc aggagcaaaa acgaaagaac
    12301 attgctcaaa agcgtttgga gctggataaa gcaaaagaaa aaagacgaaa agaaaaccca
    12361 tcatctcctg ttcagaataa ccagcagcat gttcgcaagc gtcagcaaac cgtcacgcct
    12421 gttttgtcac aaaacactaa acgtgaaaat catcagcgac cattgaaaaa agagcaaaaa
    12481 caaaagacac caccgaagca attcaatcag aggaagctaa ataaaagaac ggataagcga
    12541 ggacaacaaa catgaagcta aaaataacag tgatttgtag tgtggtcttc tctctcttct
    12601 tttttctctt attattccta gtcattttct ttgcagatga tacggacagt ggcgaaaaca
    12661 acaaggattc ttctattcca caaggcggtg tgaccgtttc accagaagtg ttggcacacc
    12721 gtccgttgat tgaaaaatat ggtaaggaat atgggattga agactatgtt tcttatatct
    12781 tagccattat gcaagtggaa tcgggcggta cagcagaaga tgttatgcaa agttccgaat
    12841 ctttgggttt accgcccaac agtttgagta ccgaagagtc catcaaacaa ggggttaaat
    12901 atttcagtga attattaacc agtgcagaac aacaaggagt ggatatagat tctgttatcc
    12961 aatcttataa ttatggcggt ggctttttaa attatgtgag gagtcatggg aaaaaatata
    13021 cctatgaatt agccgaacag ttttctaaag agaagtcggg tggtcaaaaa gcagactatc
    13081 ccaatcctat agccatacct gtgaatggtg gttggcgata taactatgga aatcagtttt
    13141 atgttcagtt ggtttcacaa tacctaaccg acacttcccc aacagaattt gatgatgaaa
    13201 ccgtgcaagt cattatggac gaagccttga aatacgaagg gtttccctat gtctttggag
    13261 gggcgtcgcc taccacctct tttgattgta gtggcttaat acaatgggtg tatgacaaag
    13321 ctggcatttc tctccctcgt gtcgcccaag atcagtatga cgctacgcaa gaaatctcta
    13381 tggaagaagc ccaagcaggc gaccttatct tctttcattc aacctataat gcaggcacat
    13441 atgtgactca tgtggcgata tatttagagg gaaatcgctt ttatcatgca ggcgacccaa
    13501 tcggttatgg cgacttaagc agtcgttatt ggcaagacca tctgattggg gctagacgtg
    13561 ttattcataa ctaagaaagg aatgaatcaa atgattataa aaatcgagcg aaaagaaaag
    13621 aaggagaaac caaagaaatc taaaaaaccg agcaagcaaa gaaaagttcc gatgattaaa
    13681 gttggcacgc ataagaaatt aacctttgtt ttatgggttt tattgattgg tagtgtcgga
    13741 tttggaatct ataaaaattt tactgccatt gacactcata ctgtccgtga aacagaaatc
    13801 attaaacagc agattgtcga taccaaccaa gtagaaagtt ttgtgaaatc ctttgcgaaa
    13861 gactactttt cgtggcaaca atcccaagaa gccattgata aacgaaatga aaaattgacc
    13921 cattatttga cggaagaatt gcaggtatta aatgaagaaa tgattcgtaa ggatattcct
    13981 actagttcta gtgtgaacga tatacaggtg tggcaggttt ctcaagtgaa tgagaacacc
    14041 tttgaggtac tgttttctgt tgaacaagtt attaccgaag acaaagacaa ggaaactata
    14101 tcgtctagct ttcatgtggt tgtccatata gatgaatcag ataatatggt tattatcaaa
    14161 aatccgacaa tgagtaagaa acctcaaaaa tcggactacc aacccaaaca gctagaaagt
    14221 gaccatacag tagatacaga aacgatggac gaaatcacta gctttttaga aacgttcttc
    14281 cagctctatc ctaccgccac agaaaaagaa ttgacttact atgtaagtaa tcatgtgtta
    14341 cctatgatta acaaggaata tgttttcgag gaattggtga atcctatctt taccagaaaa
    14401 gataaccaag taatcgtgaa tgtcgctgtg aaatatttag atcaagaaac aaaggcaacc
    14461 caaatctcgc aatttgagtt gatattagaa aaacaagata attggaagat tgtaaaataa
    14521 caaatattgg tacatgatta cagatacttt gtaatcatgt actctttttg ataaaaaatt
    14581 ggagattcct ttacaaatat gctcttacgt gctattattt aagtgactat ttaaaaggag
    14641 ttaataaata tgcggcaagg tattcttaaa taaactgtca atttgatagc gggaacaaat
    14701 aattagatgt ccttttttag gagggcttag ttttttgtac ccagtttaag aataccttta
    14761 tcatgtgatt ctaaagtatc cagagaatat ctgtatgctt tgtataccta tggttatgca
    14821 taaaaatccc agtgataaaa gtatttatca ctgggatttt tatgcccttt tgggtttttg
    14881 aatggaggaa aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcagg
    14941 aaaaactacc ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag
    15001 cgtggacaaa ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac
    15061 aattcagaca ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac
    15121 gccaggacat atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc
    15181 aattctactg atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc
    15241 acttaggaaa atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat
    15301 tgatttatca acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa
    15361 acagaaggta gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg
    15421 ggatacggta atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt
    15481 agaagcattg gaactcgaac aagaggaaag cataagattt cagaattgtt ctctgttccc
    15541 tctttatcat ggaagtgcaa aaagtaatat agggattgat aaccttatag aagttattac
    15601 taataaattt tattcatcaa cacatcgagg tccgtctgaa ctttgcggaa atgttttcaa
    15661 aattgaatat acaaaaaaaa gacaacgtct tgcatattaa gtagttcagt tttggagtac
    15721 aaaaagtgaa aatgtaaacc taaatgagta tcaaattgca actgaatttg atactcgttt
    15781 tttattgtta aatcaacgtt tgttgttaat ttacaccaca aaatgtaagt taaaatgtat
    15841 ataaaaatgc gaacgtgaaa taattattgt aggaatgcaa attggtgtgt taaaatggtt
    15901 acaatttact aagcaaaaag gggtgcattt tcctatgcaa tttcttgagc caaaaaataa
    15961 gaacgctaag tctgtggatt gggaaatatc agagcaagta agggtgatag ttaagcaata
    16021 cgctgaatat gcagagcgta ctgaaagcga agcagtggat gaattcttat tgaacatttt
    16081 agacgataag aagtttattg aatggattgc caacaagcga agtaataagc ggattgttga
    16141 aaaaatgggt atcaaagata gagtgggttg atagacaatg accaagctta aaagattagc
    16201 cacgaaggaa gatgaaattg tagatgttaa aattccgatt tcaaacgaag aattaaaaga
    16261 tagggctaaa caatatgatt tattaactcc aaaacgattt gcaacaaggt ataacaaaat
    16321 gttgttcttg ccaacgtctt ttaaatggaa cggttctgaa tatcccattc aatataatta
    16381 ctgcatcaat ccgttttgtt gtaattttgg gaaagaacag cacaaattca aagatgtaaa
    16441 agggaaacca agtcgatata aaatgactgg tagttccaaa gataaaggtc ataaaggtat
    16501 gtactgcaat gacaatccta ttggcagagg tgtatcgcaa aactgtacag taaccccttt
    16561 atctaattgg tctgttgttg aagaaattaa acgccttata gaaatcaata gcatccaaga
    16621 tgttgagcca gattatcaat ttcataaaga aggctgctcg gaagaagaat caaccccatt
    16681 caatgagccg aagcaatttt acaaaagggg gaaaagtagg ggcaaatctc aacgctatca
    16741 atgtaaggcg tgtaagaagt tcactaatgt tctaccaaaa agagaagaaa ccactactta
    16801 tcaccaacaa aagaacacca ttcttccaat gttcgctaag atggttgttg gaagggtttc
    16861 agtcagtcgt acttgtgata ttttaggaat tggagttgga acctactatc ataagctgga
    16921 gtggctgtat aggcgttgtt tggagttttt agaacgatat gagacacaac cccttcaaac
    16981 aaagaaattt aacgagatgt ggcttaatac ggacaagatg cactactacc taaataacgt
    17041 ccgtaagaaa ggacaaggtt caaagaaata tacggggttt gaagacctta atatgcaaac
    17101 gtatatagtt gtatctgctg aggtcttatc aagatatgtg ttccgttctg atgttgccta
    17161 tgattggaat atttcaatgg atgaattaaa tgaagataca agaaaattca aagaagacca
    17221 tctcaatact tttagtcgta aaaatgatag gttagattgg tcttattatc cacaagaacc
    17281 atccgctaac gatagcgaaa ataggaatgc ttatcttcac gaattaggga aaattactaa
    17341 taggagtaga tttgtcgatg gtttaaatgt tgatgctcct tatactacaa ctgctcacta
    17401 ttggttaatt aaacaaatgg ttaatgccga cgaatggcga atgattagtg atgatgactt
    17461 ctctattaga aacgcctttt atagagtatt cacgaaagaa ttaaggttga gcgatgcaca
    17521 tcattttatt tgccaagtta acaagacgaa aagcagaaaa caatgcttaa aagaattcgg
    17581 acaagcaaaa gcagaactgc tcgattgggg agatatcaga ggattcaaaa caaagtttct
    17641 acggactttg gcatctcatt atctaactga attactaaca agtcatcaat tccacgaaga
    17701 ggctattagt aaagatggag aaagatatcg caaatatgcc gataacccta tcaaacatcc
    17761 attagccact aaggataaag gattttattc agttgattgt agaacggatt tatctgctct
    17821 ggaaccaaac gaaatcgcaa agatgctatt aaatgttaat gaccattcga ccaatagctt
    17881 tattcaacag ataagacgtt atatatcatc tcttgaaaga ccattaacaa cagctcgtgg
    17941 tgataagaag agctatattt acgctaactt caatccgaag tatgctcaat ttgcgattac
    18001 aattctaaga acttactaca acttttgtcg cccttttaaa tcagcggata aaaaggtttt
    18061 gacacctgct caaagattag ggattacaga taaacaattt gattggaaag atataattta
    18121 tttcaaatga caaaaagagc ttggagtaat cccaagctct tgtgctttat ctttactgtt
    18181 cttcttcaat taacgcaccc gttaactcaa taagtttttt ctttcatata catgaaaata
    18241 gtagttatac ggatttttat cattctttat ccctttttgg gcaaatacct cattccattc
    18301 ctcataattc acttctggaa aaaaagtatc tccttcgaat tcatgatgta tttttgtgat
    18361 gtacattttc tcaacataag ggaaaaacaa attataaatc tgttctcctc cgaaaataaa
    18421 aatttcttct tcgtttttac ataactcaaa aacatcttct attgaatgaa caatttcaca
    18481 accattaaag gtaaacccct tatctctcgt cagaataata tttcttctgt caggtaaggc
    18541 tcttccgatt gattcaaggt tcttcctacc taatattatc ggatgtccct ttgtagtatt
    18601 tttaacatat tcccagtcct tgggaatcct ccaaggaatg tcattctctt tgccaatcac
    18661 tctattctta tccatcgcag caatcaaaga aactttcatt tataacacag cctttttaac
    18721 taatttacca tctaatttct tatttctatc aaaaaacctt ttctcattat cctgcccgtt
    18781 agttgaataa gcaatttttt ctataagtta tattatcata ataccacaaa atcacaaatt
    18841 ttcattcaat tatcattttc aattgcattt tataaatcat tttgtatgta atttggtatg
    18901 taagttaaaa tgaaaaaagt agtccatttt gaagtacatt ttgtcatttt gtactccaaa
    18961 actgaactac ttacttgcat atatacgcct ttatagtgga gtactacatt tacgagattc
    19021 ggttagagta tcagaaaaag aaaaaataaa agttacagaa atgtatactt caataaatgg
    19081 tgaattatgt aagattgata gagcttattc tggagaaatt gttattttgc aaaatgagtt
    19141 tttgaagtta aatagtgttc ttggagatac aaaactattg ccacagagaa aaaagattga
    19201 aaatccgcac cctctactac aaacaactgt tgaaccgagt aaacctgaac agagagaaat
    19261 gttgcttgat gcccttttgg aaatctcaga tagtgatccg cttctacgat attacgtgga
    19321 ttctacgaca catgaaatta tactttcttt cttagggaaa gtacaaatgg aagtgattag
    19381 tgcactgttg caagaaaagt atcatgtgga gatagaacta aaagagccta cagtcattta
    19441 tatggagaga ccgttaaaaa atgcagaata taccattcac atcgaagtgc cgccaaatcc
    19501 tttctgggct tccattggtt tatctgtatc accgcttccg ttgggaagtg gaatgcagta
    19561 tgagagctcg gtttctcttg gatacttaaa tcaatcattt caaaatgcag ttatggaagg
    19621 gatacgctat ggttgcgaac aaggattata tggttggaat gtgacggatt gtaaaatctg
    19681 ttttaagtac ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac
    19741 tcctattgta ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct
    19801 tagttttaaa gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa
    19861 atattgtgca aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga
    19921 aattcctgct cgatgtattc aagattatcg caatgattta acttttttta caaatgggct
    19981 tagtgtttgt ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca
    20041 gacccgtcgt ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta
    20101 gtgcgtttta tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta
    20161 atatagattt gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat
    20221 tgtgtcctat ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa
    20281 atttcccttt atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga
    20341 atataataac aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa
    20401 actctcatct tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt
    20461 cttgcttcat tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag
    20521 ctatccatgt atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt
    20581 aagaaaaatt catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta
    20641 cttgggtgtt gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt
    20701 gttcccactt gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca
    20761 acggctctaa aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt
    20821 tactgttcct atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc
    20881 ctatagaaca ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct
    20941 gattttattt acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa
    21001 ttggcgactt aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct
    21061 taattaagtt cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg
    21121 tggttttgca gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt
    21181 ttatgtgctt ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac
    21241 aaatcccccg ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc
    21301 ctttcacgac agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga
    21361 aaatcagttt gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa
    21421 acatctagac agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt
    21481 caactctttt tctactgttg atacgtacgt cactgatttc caactattta aattatttgg
    21541 attaagaatc aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa
    21601 aaaacggaac attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga
    21661 gttactaaat ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat
    21721 taaagaattc atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc
    21781 ctcattgtaa aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat
    21841 cgagggtata cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat
    21901 atggttattg atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta
    21961 tcgttcgaaa ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt
    22021 cccgatttgg gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata
    22081 ggctaacaag ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa
    22141 gaaaagagct gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag
    22201 taattcgaaa gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata
    22261 ctcccgtata gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg
    22321 agaaccatgc agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata
    22381 ttcttctctt gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg
    22441 agttttccta gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt
    22501 tagttgagct aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat
    22561 tgatgattac aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg
    22621 aagctgttga agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt
    22681 tgcacagtaa ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac
    22741 ctattatttt cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg
    22801 tagtggattc actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa
    22861 accagaagaa ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat
    22921 tttccttcca tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac
    22981 tattaagaag catttctctt attttgataa aatggctgtt tccgatattg agcctattga
    23041 tgtgcaaaat tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt
    23101 tcaaggacta ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa
    23161 tccatctaag attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa
    23221 agaagaattt gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt
    23281 atttatctct ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat
    23341 tcaatgggaa gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta
    23401 caagaatcaa tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat
    23461 tgtattagat aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct
    23521 aattcagaca gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc
    23581 atatgctatt gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt
    23641 acggcattct catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga
    23701 tcgtctagga cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa
    23761 cagtaatttc gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca
    23821 aaattatgat acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat
    23881 gaaaaaagtg caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa
    23941 gaggatttct agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg
    24001 atgtcataca cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt
    24061 tgcaagactt cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca
    24121 gcacggatac cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg
    24181 atccctggaa gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt
    24241 tcttcacgca agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg
    24301 ccaagaacac gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca
    24361 ggcatgccta actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt
    24421 tcgatcaatt caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc
    24481 gtctgtgctg tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg
    24541 aaagagacgc cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat
    24601 tcattcccaa tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag
    24661 aagcgatctc ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc
    24721 attacttgtt ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat
    24781 tggtcaccga tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa
    24841 agaccaagca agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg
    24901 ataaagttat ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc
    24961 agcaattcga ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa
    25021 ttacgtgttg tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca
    25081 aagccttcag gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt
    25141 ggtgtttctt taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat
    25201 tcgcggtttt tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca
    25261 tagcaaatcc ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca
    25321 ttctcatcat atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct
    25381 tttacttctt ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga
    25441 atacgtcttg tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca
    25501 acgttcgtca tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc
    25561 tcaaaatagt caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat
    25621 atgattttca gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc
    25681 ctgtaaattt ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc
    25741 acgatctccg atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag
    25801 taacgcacca aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata
    25861 gacagaaaaa tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt
    25921 attgatcccc tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat
    25981 gatatcatac acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac
    26041 acggttcaag aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc
    26101 catgattcca cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag
    26161 ctgaacgttt cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt
    26221 actttttcca acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta
    26281 tttatgaaga aataaacctt tactcaagaa caaagattca aattctttca tataaagata
    26341 aatcagatga gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt
    26401 caaacttttg atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa
    26461 ttctcgccat tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc
    26521 ctgcgttctc ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta
    26581 agagagattg ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct
    26641 tgacttgcgc acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg
    26701 gaagacatga caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa
    26761 tgggcttttg agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca
    26821 cgtgaaacag tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta
    26881 cctgatgaag gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg
    26941 gcatttgcga cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta
    27001 gcgcaaggaa acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc
    27061 tatgaagcag ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct
    27121 ggaaaaaaag ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt
    27181 acactgacaa ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa
    27241 gaaacatttg gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt
    27301 gtgaaagaat aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat
    27361 gtacgggtac gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt
    27421 ttcttcaaaa agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat
    27481 acagaacttg tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca
    27541 tactttcctt ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag
    27601 atagaaggga gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt
    27661 atcagtaaca actgtctcac agattttgaa taacaaagga agcagattta gtgataagac
    27721 aagaaaaaaa gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc
    27781 aaatgtgatc aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt
    27841 cttcttctcc aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat
    27901 catgttatgc aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc
    27961 ccaccgctca gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc
    28021 attagacagc ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg
    28081 tcgggataac ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct
    28141 gtttattgaa aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca
    28201 attagaagaa cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa
    28261 tgggaaattt gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa
    28321 attactcaaa cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg
    28381 atgctatcag gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg
    28441 attcgatgga ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc
    28501 aagttttgat attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca
    28561 aagaaaagtt ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt
    28621 attgacaaag ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta
    28681 gtgctttttt tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa
    28741 tgtataatac cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa
    28801 aaaaagaggg atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat
    28861 acgaatggaa agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa
    28921 gacatgacaa atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc
    28981 atcgatttga gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt
    29041 gacacgacca cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa
    29101 atgtctggac gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc
    29161 ggatttcaga tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta
    29221 gcggtaattg gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt
    29281 gacgtcacag cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa
    29341 atcgcggcag gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg
    29401 aagaacatcg aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct
    29461 aaccgagaaa cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga
    29521 aatagtctag aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta
    29581 gtagaaatgt gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca
    29641 accgatgaag caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa
    29701 ttcaaagaaa tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga
    29761 attttgactg cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa
    29821 atcgtggcaa atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa
    29881 gaagaagata ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg
    29941 acaaaaggtg agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt
    30001 gaattattat ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat
    30061 gacatcatca cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa
    30121 gaaatggaac taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa
    30181 gctgtgatgc aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat
    30241 ccagcatggg ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca
    30301 gtaatcgggt ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat
    30361 gccatcaata acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca
    30421 aaggatgatc aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt
    30481 gcgcttgtca aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct
    30541 tgcgaaatag caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca
    30601 ggcggagcaa aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt
    30661 gtcaaagctt ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt
    30721 gctacaagaa tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc
    30781 tattaatttt ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag
    30841 ctattgaagc gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc
    30901 tcgtaacgga atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat
    30961 taacgaattg tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat
    31021 ttacgcatct agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc
    31081 gacaagtaat ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg
    31141 gtgtgacgaa ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc
    31201 tataactcat gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg
    31261 ctttacagaa atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg
    31321 gttattttta ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc
    31381 atcagacaca acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt
    31441 caaccagtca gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg
    31501 aaatgatggc ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga
    31561 ccaagcattg aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc
    31621 gatccaagaa caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat
    31681 cgatggatta gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc
    31741 aggtgttgca gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa
    31801 aggtgaagta atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa
    31861 agaattagga aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga
    31921 taaaggacgt tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc
    31981 ttcaggcggt acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga
    32041 caaaaaagtt tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt
    32101 aaacggcgaa aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt
    32161 agaagatttg gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta
    32221 tggattgaaa gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa
    32281 agcagttgat gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc
    32341 tgaagaaaac taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt
    32401 ttgattagaa caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga
    32461 acaactccct gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag
    32521 tcaatcgttg ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata
    32581 ttgtcataga aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca
    32641 tcaatttaca agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca
    32701 aatctacatt gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga
    32761 tgcgtgggaa agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga
    32821 tggtccatca gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg
    32881 gaagcgagcc tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa
    32941 aagtttctga acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag
    33001 ttggaatgga acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga
    33061 tttttgatga gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca
    33121 tgaaggaatt agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa
    33181 tcaaagcagt agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca
    33241 acgttaaaga cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat
    33301 tcaaaacgat gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag
    33361 tggtgaaaga aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg
    33421 gcgaagtact tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt
    33481 tgactggttt gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta
    33541 ataaaaaacc ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata
    33601 aatacgggtt agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc
    33661 aaaaacctta cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag
    33721 aattgatcga agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat
    33781 caggcggaaa tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt
    33841 tgattgttgc aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac
    33901 gtctgatcga acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag
    33961 aaattttaaa tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg
    34021 ttgatccgaa agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag
    34081 aagaagcacg aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa
    34141 ttccgtaatt tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata
    34201 ttgatggctg cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc
    34261 cttggttcgc aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca
    34321 gcattaggtt tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa
    34381 gcactttgcg ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa
    34441 gttgttttgc ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct
    34501 gttccaggtt tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg
    34561 aactatatct tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat
    34621 cgctcaagtt tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg
    34681 ttaacacaag cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg
    34741 cttattttcg tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt
    34801 ggattgaatc cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg
    34861 tcgatggtca tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg
    34921 tatcaaaact tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt
    34981 tccttgcttg gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt
    35041 ttgaagatcg gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat
    35101 gtatcgattg ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg
    35161 gctaaagtct tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca
    35221 acgaatcaaa accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga
    35281 tccttacttc tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt
    35341 tttctgaacg aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt
    35401 ttagttccat cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga
    35461 tcgctatttt agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa
    35521 cagtcagttt gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg
    35581 ctttaggtgt tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc
    35641 agaatctggg gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt
    35701 tctttaaaaa tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg
    35761 tgatcatctt caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag
    35821 ctgccgacac attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg
    35881 gcttgttagg cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag
    35941 ctggtacgat cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga
    36001 atcctttagg ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca
    36061 ttggcgcaca attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat
    36121 atttgctgac aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa
    36181 acgggaagaa ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg
    36241 gaagtgtttt tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg
    36301 aataatcatg tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc
    36361 accagatgcc gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga
    36421 agcaggattg aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga
    36481 aggtgtggaa tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt
    36541 agggaaagat acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt
    36601 tcgtgtgttt cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg
    36661 ccgtaaagtc gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga
    36721 acaccaaatg aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat
    36781 cgcggcacat gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg
    36841 tgatataaca aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg
    36901 tgaaccagga gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg
    36961 acacacagtc cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat
    37021 caacgatatt ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga
    37081 tggcgttgac cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa
    37141 tttagtagat tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc
    37201 gattgaagaa tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt
    37261 gctaatgatt acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg
    37321 agaatatgta ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca
    37381 aggatattat tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga
    37441 aaatggcgaa agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg
    37501 accaagttaa gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa
    37561 gaaattgatt ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa
    37621 gatgctgtaa tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg
    37681 catgcaggaa agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg
    37741 agattccact actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg
    37801 gccggactag gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca
    37861 tttgtaccag gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct
    37921 ttgatcggtc cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat
    37981 acgcctgact atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa
    38041 gaaggcgtat atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg
    38101 tctcggacaa taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca
    38161 gctcacagcg gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg
    38221 caagcgaatc tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt
    38281 aaagcattgg ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt
    38341 gcatattgaa gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt
    38401 gcgagctaaa tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg
    38461 cggtatgtta ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg
    38521 aatgggaatg ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa
    38581 gaaattgatt cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt
    38641 agtactagca caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt
    38701 tcattttcct ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga
    38761 aaagggctat acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga
    38821 tttgacagag acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc
    38881 ggctctttat tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag
    38941 cgaccatttg atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga
    39001 aatgatcgaa gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc
    39061 cctgcattaa gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct
    39121 ggacttatta ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc
    39181 cgtttaactc tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata
    39241 ttgtgacttg tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt
    39301 gacaaatcgt gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa
    39361 ctgttaagtc atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca
    39421 tgatgtcttc gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat
    39481 gtgcagctac taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga
    39541 atggcaaagc gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt
    39601 ctaacattgc gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg
    39661 gaggaagagt atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag
    39721 ctgtttcttt tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg
    39781 atttgatttg tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga
    39841 ttgcacgagt taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt
    39901 tgcgtccgcc ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac
    39961 cagtaaattg gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg
    40021 gcatgtttag ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta
    40081 ttgtacaatt ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac
    40141 aagt
//
LOCUS       DACDMM010000008        38925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDMM010000008 DACDMM010000000
VERSION     DACDMM010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.008"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(10..1764)
                     /gene="ade"
                     /locus_tag="IWD16_000315"
     CDS             complement(10..1764)
                     /gene="ade"
                     /locus_tag="IWD16_000315"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP9087402.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            1896..2825
                     /gene="cpaF"
                     /locus_tag="IWD16_000316"
     CDS             1896..2825
                     /gene="cpaF"
                     /locus_tag="IWD16_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9087403.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            2782..3831
                     /locus_tag="IWD16_000317"
     CDS             2782..3831
                     /locus_tag="IWD16_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP9087404.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            3828..4106
                     /locus_tag="IWD16_000318"
     CDS             3828..4106
                     /locus_tag="IWD16_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP9087405.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            4081..4551
                     /locus_tag="IWD16_000319"
     CDS             4081..4551
                     /locus_tag="IWD16_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP9087406.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            4568..4870
                     /locus_tag="IWD16_000320"
     CDS             4568..4870
                     /locus_tag="IWD16_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087407.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            4887..5258
                     /locus_tag="IWD16_000321"
     CDS             4887..5258
                     /locus_tag="IWD16_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP9087408.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            5230..5589
                     /locus_tag="IWD16_000322"
     CDS             5230..5589
                     /locus_tag="IWD16_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087409.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            5761..6768
                     /locus_tag="IWD16_000323"
     CDS             5761..6768
                     /locus_tag="IWD16_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9087410.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            6793..7977
                     /locus_tag="IWD16_000324"
     CDS             6793..7977
                     /locus_tag="IWD16_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP9087411.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(8414..9997)
                     /locus_tag="IWD16_000325"
     CDS             complement(8414..9997)
                     /locus_tag="IWD16_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9087412.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(10575..10718)
                     /locus_tag="IWD16_000326"
     CDS             complement(10575..10718)
                     /locus_tag="IWD16_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP9087413.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(10940..11419)
                     /locus_tag="IWD16_000327"
     CDS             complement(10940..11419)
                     /locus_tag="IWD16_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9087414.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(11492..11776)
                     /locus_tag="IWD16_000328"
     CDS             complement(11492..11776)
                     /locus_tag="IWD16_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087415.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(11854..12048)
                     /gene="ssrS"
                     /locus_tag="IWD16_000329"
     ncRNA           complement(11854..12048)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWD16_000329"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(12120..13406)
                     /locus_tag="IWD16_000330"
     CDS             complement(12120..13406)
                     /locus_tag="IWD16_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP9087416.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            13544..14215
                     /locus_tag="IWD16_000331"
     CDS             13544..14215
                     /locus_tag="IWD16_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP9087417.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            14181..14669
                     /locus_tag="IWD16_000332"
     CDS             14181..14669
                     /locus_tag="IWD16_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP9087418.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            14682..15629
                     /gene="prmA"
                     /locus_tag="IWD16_000333"
     CDS             14682..15629
                     /gene="prmA"
                     /locus_tag="IWD16_000333"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP9087419.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            15630..16394
                     /locus_tag="IWD16_000334"
     CDS             15630..16394
                     /locus_tag="IWD16_000334"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP9087420.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            16627..18840
                     /locus_tag="IWD16_000335"
     CDS             16627..18840
                     /locus_tag="IWD16_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP9087421.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            18851..19297
                     /locus_tag="IWD16_000336"
     CDS             18851..19297
                     /locus_tag="IWD16_000336"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP9087422.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            19468..20727
                     /locus_tag="IWD16_000337"
     CDS             19468..20727
                     /locus_tag="IWD16_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP9087423.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(20816..21763)
                     /locus_tag="IWD16_000338"
     CDS             complement(20816..21763)
                     /locus_tag="IWD16_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9087424.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            22157..23449
                     /locus_tag="IWD16_000339"
     CDS             22157..23449
                     /locus_tag="IWD16_000339"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP9087425.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            23464..25233
                     /gene="aspS"
                     /locus_tag="IWD16_000340"
     CDS             23464..25233
                     /gene="aspS"
                     /locus_tag="IWD16_000340"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP9087426.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            25342..26217
                     /locus_tag="IWD16_000341"
     CDS             25342..26217
                     /locus_tag="IWD16_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9087427.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            26483..28366
                     /locus_tag="IWD16_000342"
     CDS             26483..28366
                     /locus_tag="IWD16_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9087428.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(28441..28725)
                     /locus_tag="IWD16_000343"
     CDS             complement(28441..28725)
                     /locus_tag="IWD16_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9087429.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(28715..29449)
                     /locus_tag="IWD16_000344"
     CDS             complement(28715..29449)
                     /locus_tag="IWD16_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP9087430.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            29578..30201
                     /locus_tag="IWD16_000345"
     CDS             29578..30201
                     /locus_tag="IWD16_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP9087431.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            30393..31514
                     /locus_tag="IWD16_000346"
     CDS             30393..31514
                     /locus_tag="IWD16_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP9087432.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            31521..34649
                     /locus_tag="IWD16_000347"
     CDS             31521..34649
                     /locus_tag="IWD16_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP9087433.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            34668..37862
                     /locus_tag="IWD16_000348"
     CDS             34668..37862
                     /locus_tag="IWD16_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9087434.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(38008..38715)
                     /locus_tag="IWD16_000349"
     CDS             complement(38008..38715)
                     /locus_tag="IWD16_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9087435.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        12615 a         6979 c         8227 g        11104 t
ORIGIN      
        1 ttatttgatt taaaatgtat ttatttcatc ggtttttcca caaggtggaa tcaattggaa
       61 gcgagaaaat gaaaataatc ctttgtcggt gatcttcaat tcgggtatga cagataatgt
      121 taagaaagaa agtgtcaaaa aaggatcaaa ggttttagct gctcctagtt gatgtgcttt
      181 agttattaat tcattcaact gttcatagac gatcgaaaaa ggttcctgtg tcatgattcc
      241 gcctatagga agagaaacac aagctatttc ctgctgtttg ttgacaacgg tcatcccgcc
      301 acctttttga atcaaaagat tagcagcata cagcatatcc tcgtcgtttg tcccaacgac
      361 aactagatta tgcgagtcat gggcaatcgt tgtcgctatc gctccttgtt tcagctgaaa
      421 tcctttcact atgcctaatc caatgtttcc cgtttgatgg tgacgctcga tcaccgcaat
      481 ctttaattgg tctttttcga ttgaaggaat gaatcgttcg ccttgtcggt ccactttttc
      541 taccaaatct ttagtcacta gactattcgg gataatttcg atcacatggg cttgtgaaga
      601 agaaagaagc agctctaacg aatgtggatt taatggttga gcgtttagtt ttggcagaca
      661 acacgtatat gaatttgaat gatcagcaaa taaatggtct tggagttgcc cagattgaac
      721 aactaatgtt cccttataga aaacttcatg aattggcagc ttggtaagat cgtctgttaa
      781 gaagaaatca gcttgatacc caggggcaat cgcacctagt gttttcaaat caaaacattc
      841 tgctgcattc aatgttgcca tctgaatggc ttgtaatgga tcgattccaa gtgcaatagc
      901 ttgacggacg atatgatcga ttgatccttc ttttactaaa tcattgatca gtttatcatc
      961 ggtgacaaag aagcatcttc ttgaattttc aggtgtgatc gccggtagta gcgatgccag
     1021 atcttttgca acggtccctt ctcgaaccat taaatacata ccaagatcca aacgttcttt
     1081 agcttctttt gcagttgtag cttcatgatc cgtccgaata cctgctgcag gataaacatt
     1141 taactcttcc atgccgatac cagctgcatg cccgtcaatt tttttcttat gctggtgcat
     1201 caattggatc ttttctagga tgtctgtttc attatttgct actgcttgat agttcatgac
     1261 ttcagctaag cctaaaactt cgggtttttg taaaaaaggg gctaattttt ttgcatttaa
     1321 atttgcacca ttggtctcaa aaggggttac aggtacacaa gaaggtagca taaaaaagat
     1381 attcatgagt gtttgtcttg catcttctat cataaactca agacctgtac tcccagcaat
     1441 gtttcctatt tcatggggat ctgtcactac agtagtaact ccatgaagca aggatacttt
     1501 tgaaaattcg cttggtgata aaagtgagct ttcgatatgc acatggccat cgatgaatcc
     1561 tggcactaga tattgcccat tagcattatg tacgcttttt ccttcgtatt caccgattcc
     1621 tacgatcatt ccttcgcaga ttgctacatc tttttccatg atttctttcg taaagacgtt
     1681 gacgatctta gcatgcttga tcactaggtc agcaggcttt tttttacttg ctacttcgat
     1741 atatttcctt aatgttgttt ccaatggagt gcctcccaag ataaattttt ataagtataa
     1801 cataaattcg gcttttgcca atattttttt gtaaattcat tttatttgaa acaaaaagat
     1861 caaaaagacg tatgtttttt gtggagggga gaataatgga aataaaacga ttatcggatc
     1921 aaatgataag tgaaagtttt gccaaacatg cccaagacgt atatatattg ccagcttcag
     1981 aagtatttta catctatttt cgctatggaa aaaaacgaac attctggaag aaagtctctt
     2041 cggcagatgg acagcaactg atctcaagat ttaagtattt gggacagatg gatatcggag
     2101 aaaaaagaaa agctcagtta ggtgcattta cttattcttt agaaaaacag gaagttcgtc
     2161 tgcggttgtc ttctgtagga gattaccttc aacgagagag tttagtgatt cggctattat
     2221 acggtacttt tgaaacaccc catcgttgct ttagttctcg agattttgca actattttac
     2281 agaaaaccgt cagacgtgga ttataccttt tcagcgggac agtcggatca ggcaagactt
     2341 ctctaatgta tcggttggct ttggaagaaa aattgcaagt gattactatc gaagatcctg
     2401 tagagataga agaaccgttt tttctgcagc tacagatcaa tccgaaaata gcacagactt
     2461 atgatacact tttgaaactc gcattacggc atcgtccaga cttgctaatc attggcgaga
     2521 taagagatga atcaactgca catgctgcgg taagagcagc actaacaggg caccgggttt
     2581 ttgcaaccgt acatgcacgc aatcttgaag gtacattggt cagaatccat gaactgacaa
     2641 agaaaaaaga agagctatct gaatgtttag ctggtgtcat ttatcaagaa cttttggaag
     2701 atgccaatca ccatccttct gttttatgga gttatcaatt tatggaggaa gaatcagtaa
     2761 aaaaatcatg ggaggaaagt tatgaagaag ctcaggaaaa tatttggcag tcagaactat
     2821 tctaaaggat taacaagaag ccagcaaaac cagtttgcac aaaccatggg agaactgctt
     2881 ttatctggtt ttagtctgca agaatccctt tatttattac tcacgatccg ttcgtttcca
     2941 cctgttattt tggcaaatat ccaacatcag ctaaaggaag ggcaagcgtt tagtgaggta
     3001 ctttcgcaaa taggatatgg agcagagcaa ctggtacaga tcgagttagc tgaacgtcat
     3061 gggaatctgc cgacaacttt gttggaaatt gccaaacagt ttcggcttgt tgaaggattt
     3121 cgcaatgatt tgaaaaagct gcttgcatat ccgatgctat tgctcacctt tttgctaggt
     3181 atcttgttta gtatgcggtt gttcgttttg cctcagctta ttggttcagg aatggtcatg
     3241 caagagcatt ggggtattca gctgatacaa gtctttcatt ggtatgttct actattcatc
     3301 ctagcggtta ctctattggc tttactagta aggtatcgtc tgatgaagtt gtctcctgtt
     3361 gatcagagtg aatggataag cagacaatta ttgattggac cgatatattc cttgtatcaa
     3421 tcggcttatt tgtcactaga gattggaaag ctttttcatg aaggtttcga attaaggcaa
     3481 atcatagtct gcttaaagga aacgaaacaa gaaagtctga ttcagcatct tgcctctaag
     3541 ttgattcaag gattggaggc aggtatgtca ttggctgagc agttccagaa ttatcgtttt
     3601 ctatcagaag aattcagtaa aattgtttta cagggagagg caaagggaag tttaggtaaa
     3661 gaactgattt tttacagcga atggacaaga aaacagcttt tcaaacgtct tcatcactgg
     3721 atgcatttta ttcagccaat catttttttg ggcgtagcgg ctttgatttt attagtttat
     3781 gccgctgttt tattacctgt ttacggaagc atagaggagg ttttgccatg aaaaagagaa
     3841 aaatatctta ttatagcggt tttacattga tcgaaatgct aatcgtctta ctgatcatca
     3901 gtgtattagt gttgttgttc gtccctaatt tgtcgcgtta taggaatcat gtagaccaag
     3961 aaagtcgtga agcgatcatc cagttggtag acacacaaaa agagctctat gcgcttcaaa
     4021 ataatggaag agttcctact gtggaggaat tgttgaatga aggctatatc aaacgagaac
     4081 atgcagaaat ctatcagcgg ccttgaaggt aaggttttag catcaggata tacgttgttt
     4141 gaatcactct tactgatttt attacttaca acgatccttt cttttccagt tctcgcattt
     4201 tctgcatgga agaatgaatt ggcagtctat caatttttcg gccaatttga aaaacgaatc
     4261 tattctacgc aaaaaatcgc tattgtgaat cagatccaaa caggctttta ttggaatagt
     4321 gaagagaatc aaatcatttt tcatgttccg aatccagata aaataaattg ggaagtatta
     4381 gatattcctg atgagattac cttgaaacgc catgcttcta ttacttttgc tgctagaacc
     4441 gggaatgaaa gttcactaaa agcttatcaa ttttattggg aagaaaaaga gcggacgatt
     4501 acgtatcaat tccagatagg gagtggtcgc tatacaaaaa gaattgaata aaacaggatt
     4561 tgttttgttg gagtccctta cagcgcttat gatcgcttct gtctgtgtct acttagtgag
     4621 ctttggccag ctgtttttgc ttcaggcaga aaagaaaagt ggggaggaac tgctatttat
     4681 tcgaatgatt tatgaagata tcaagacgca gcggatgtat cacaaaagac cagagcgaag
     4741 ggaagaaatg atcccatacg atatccaaat tgtcgataat aatggagtaa tcgaaaaagt
     4801 gtttgtcaat atggagaaag aagcgatcag tattgaaaaa acaagtaatt ccagcattta
     4861 cgctgattga atgtctaatt ggactgatga tattgagcag tctattgctc acattcaatc
     4921 ttttgattca gcaaaccatc caagtaagtg aatttttgca gcgtaaagat caaaaagagt
     4981 ggctaatatt tttagcacaa ttagaagaag aactcaagag cagtcacaat gtctctgtga
     5041 aaaatcagca actgcactac acgatcgagg ataaacagta tatcatcgaa agatatcagg
     5101 caatgatacg taaaagaaga acttccggcg gacatcaacc tatgttaaca agtatcactg
     5161 agcttcaatt attggaaaaa gaacacacaa tttcttttta tgtacacttt gaaaatggag
     5221 aagatggata tgccatatgg acaaaaaacg atcagtagaa aatgttataa gggctctatt
     5281 ttattttctg tcttgctttt atttctttta tgtacgtttt tattgatggc agcattagaa
     5341 agctaccgtt tgtcaatgga tcttaatata cgaatcaaaa attattatat agcaaaaata
     5401 atgaagcaga tggctttatc ggaacagtct acgcttgccg aaagttcgga aggagttttt
     5461 tactacacaa ctggatctgt aacatatcag tgggtacaac agtccctatt cttagaggtg
     5521 gaagtctcac cgtttatctt tcggttcaca gaagaagtaa aaaatgatac agatactagt
     5581 acagaatgat ggaatgttcg tgaaaacgcc ctcttttctg ttataacact gtttttaata
     5641 aaaaaatgtt tgccgtttca caaataaaaa acagtgaaaa cgattgaaat tttgtcaatt
     5701 gttcgataga attaaacagg tgaaaaaatt gttataatat atctagaaat gaggtgcacc
     5761 atgttaccag aaaaaatgga acaagggttt aatcaaaacc tggaagctat tcagctttta
     5821 cagaatgcat tgggaacttc ctttttagaa gcctatgtcg aaaatgcaga aaatttaata
     5881 gatgattatc aagtacgtgt ggtggatgga gtgccgacaa aagagacaac gcaacggatc
     5941 acagctcttt atgaagagtt gaagaaactt tctttcgagc cagaagaatg gcgcagactg
     6001 tctcaattac ttttattgaa gggcagccag acagaacatt tgcaggcgaa ccatcaactc
     6061 actcctgata gtatcggttt tttatttgtt tttcttattg aacaactata cacaaataaa
     6121 aaagcaccgg ttaagattct ggatattgcg gcaggaatgg gcaacttgtt attaacagtt
     6181 cttttaaacc tatcgaatgc ggggtaccaa acagaaggaa ttggtgtgga tattgatgat
     6241 acattgttgg ctgtcgcagc ttctacaagt gatttgacac aagcaaatgt tcaatacttt
     6301 catcaagacg ggttgcagga attattaata gatcctgtcg attttgcaat cagcgatttg
     6361 ccaatcggtt attatccgaa tgacgaaaaa gcaaaagagt ttttaacaag taccgaagaa
     6421 ggccatagtt atgcacatca tttattgcta gagcaatcaa tgaaatatgt gaagccggat
     6481 ggttttggtt tgtttttgat gccgagtgga tttttagaaa cggatcaaag tgaagaaatc
     6541 aaaaaatggt ttaaagaaga aggttatctt caaggaatga ttcaattgcc agatgaattg
     6601 ttccgtaata aacaatcgca aaaaagtatt ttgatcttac agaaaaaagg accacaagca
     6661 caacaagtaa aagaagtctt gcttgtgaaa cttgcttcat taaaagaacc agagaaggtg
     6721 acggaatttt tcaacgagtt taaaaactgg aaatcatctg ctctgtaatc attcaacgag
     6781 aaggagagtc atatgtctaa aacaattgca atcaatgctg ggagttcaag tttgaaatgg
     6841 caattatatc aaatgccaga agaaaccgta gttgcgaaag ggatcgtcga acgtatcggt
     6901 ttacaagatt ctatttttac catcaagtac ggcgataatc aaaaattcga acaaatcatg
     6961 gatatcgaaa accacgaagt agcagtaaaa atgctgttag atcagttgat tgaactgcaa
     7021 attttaagct cttatgatga aatcaccgga gtagggcacc gtgttgttca cggaggagaa
     7081 acttatgggg attcagtagt gatcgatgag aaagtaatgg accgcattgc tgaattagca
     7141 gaatttgctc ctttgcataa tcctgcaaac ttaatgggga tcaaagcgtt taaaaagatt
     7201 ttacctgaca ttttgagtgt ggctgtattc gatacatctt tccactcaac aatgccaaag
     7261 caaaattatt tatatagctt accggtgtct tattatgaag atttcaaagt aagaaaatat
     7321 ggcttccatg gcacaagtca tcgttatgtg tcggaacgtg cggcaaaatt attgaacaaa
     7381 ccgatcgaag aactaaaaat catcacttgc cacttaggaa atggtgtatc gatcacagca
     7441 gtcgatggag gtaaatccat ggatacttct atgggattca caccattagc tggggtgaca
     7501 atgggtacac gttcaggtga tatcgatcca gctgttctcc catatttaat ggaaaaatta
     7561 gagattgata ttgatgaaat gatcaatgtt ttgaacaaga aatctggtct gctaggccta
     7621 acaggattgt ctagtgatat gcgtgatctt gaaaagaact atgaaaaaga acatattcgt
     7681 ttagcttatg acattttcgt ggaccgcatc cgaaaataca tcggcggtta tgtgacaacg
     7741 atgaatggtg ttgatgcaat tgtctttact gctggtatcg gcgagaatga cggacatgta
     7801 cgtagtgaaa tcatcaaagg tatgtcttgg tttggttgtg aaatcgatcc agcgttgaat
     7861 gaattgcgtg gtgaagaagt agatatctct acaaaagaat caaaagtaag agtattagtg
     7921 atcccaacag atgaggaact aatgattgcc cgtgatgtgg agcgtctgcg cggctaagcc
     7981 aaagcattcg ttaggataaa agatgaaacg catgcaagag ggaattcttg catgtgtttt
     8041 ttttgcggag aagaccgcaa aatggtaatt taacttcaat ttcccttctt ttttacagga
     8101 tcttcttagg gaattttgct tggaaaagtg gtcaaaaggg aaaagactgg cattttattt
     8161 attgattttc aggaaaatcc caatatttta ttttcattca tctttataag ggacattaca
     8221 tatgtaaaaa gaaaaaacat ttttagatca gcctcgtggg ctgtcgactt tattcttcgc
     8281 tttatgttta gctagaaaac cgatccgaaa cattcattga tcgaataata aaaacttgat
     8341 tttccacctg tactttgcct gaatcgtgaa agcacaggta tgaaaatcaa gttttttgtt
     8401 gcgaaaaaac gtcctagtca atcagttggc cgagcggctg gcggctgatc tgctcctgat
     8461 caataacgag tgcttcttgg aagtgttgct gcaaaaagta gcggtcatgg ctgactgcta
     8521 ggagcgttcc ttgatagtct tgcagcattt cttcaatttc ttctcgagca tagctgtcga
     8581 ggtgattggt tggttcatcc aaaatcagga cgttgatttt ttctaaaaag agtttcagca
     8641 acgccaaacg gattttttca ccaccggata aatctttgat ccgtttaccc acatcgcttt
     8701 gataaaagcc acttttggct aattgccgtc gagcgacttc ttcgtttcca gtcaatgtga
     8761 tggcgtaagc cagcaaacgg ctatcgggat cggcaaactg cagctgctgc ggcaaataac
     8821 cgatttgcaa gctactacct aatttgatgg tgccaccgta aggcttttcc tcaccgagta
     8881 ataatttgag taaggttgat tttcctgcac cattgtcgcc gatgatggct aaatgatctc
     8941 cacgatagac tgcaaaactg ctgtctttga atagtacttg atcagcgtac tgctggtgca
     9001 agttttgaat gatgaaaact tctttgccag attttccacc attagacaag ctttgcagcc
     9061 gatttttcgg gggaggtggc ggtttgacga gtgtcagttt cgccaggcgg cgttccagtt
     9121 ctttggcttt tttgaagaag gactcattgt ctccttcatg tgcccactga cgaaaacgac
     9181 gaatcatgac tttcaaccgc tggacttctt tttgctgcag ttcgtaattt tttgtcagct
     9241 cagccaagcg agcttctttc aagactcgaa aacggctgta gtttccggga tactcaatca
     9301 atgcaccttc ttccagttcc catgtttttg tggtgacttg atcgagaaat tgccgatcat
     9361 gagaaacgac cagaaccgat ttttttgtat tctgtaaata gttttccaac cagcgaatac
     9421 tgttaagatc taaatgattg gtcggttcat cgagtaaaag taaatcggca tcggctgcta
     9481 aaattttggc taaagcgact ttaactcgtt cgcctccgct aagttctgtt aaccgtgctt
     9541 cctgacagtg tggaatccct aagccttgca agatcgctgc aatccggtct tctaatacgt
     9601 aaccgcctag ttcttcaaag cgatgctgca gctcaccgta acgggccaga cacttctcta
     9661 agtttgtatc tggttctgtc atcaatgctt cgtagcgact catttgctgt ttgaggtcct
     9721 gtatctcagc ggcagaatgg agcaagtagt ggttgacggt cgcttcggaa actgtaagtt
     9781 cttgttcgac cgtcccgatc tttaagcctt ttttgcgact gataacaccg ctgtcgacac
     9841 cttctctgcc ggtcaagatt tgtaaaaagg ttgatttgcc agttccgttg atatcgacta
     9901 aggcaatttt ttcaccagct tcaatttgac aggataaatc ctcaaataat ggattcccgc
     9961 cgattgtttt tttaatagaa attgcttgta tgatcatgat ctctctcctt gagaccactc
    10021 aaaaaggcca tggttatccc tggcctgtgt tatttaaact cagattatca tgagaaaaat
    10081 accttgagat ggccattgat tttacgtgaa ttcaactgtt ttggacaaac ttgtcccgtc
    10141 aacagcaact gtaaaaccaa ttgcatggct aaatgcgata tggtatcgtt ggtaattttc
    10201 attacttgtt attttagtca tgcgcatcat agtattttcc ctccttcgtg tagttgtctt
    10261 ttccagtgta cctgaaagtg tcagaaagtt caactagcgc tgacatttac ttaatattta
    10321 tcgatgtgta attctgtcag agagcttttt ttatctgact gagggtaatg tctcgtggac
    10381 cacatcattg tattctgagc taaaccagcg ttcaggcgaa atcatatggt gtttgataaa
    10441 aaactttgtc tacatgactt ccttttgcca tttgcttaat accgttgcat taaacagctc
    10501 ttctctttgg aaatctcacc aacatcgcgt tcaggagtat cgttgtggtt gcaatggcaa
    10561 gcggtagcct ttcatcaatt cttgaatgat ttaccgaatg tagcacttgc cttctcgcgg
    10621 tacccgaacc cgaaataaaa ttttagtttg gcgataaaat cggtatccgc catcgatact
    10681 tcatattttt tcgtatcaga ttcatcggaa acttccacat tgacgaacca ataagctttg
    10741 gcatgcttag gtcgttttaa accacaccag ggcgactaat cgccctggtg ttcacttttt
    10801 ttattttact tgaactcatt gactgttttc cctttatttt aaacattata aagaccgatg
    10861 agtgcttgcc gcgtcttttg tttttatcac ggttttttgt gcaaaaaaac ctgccgatag
    10921 ttggcaggtc gtctacgtgt taagctttgg tgtggtcttt agctggcaat tggttttcta
    10981 aatcagttcg gacaacaagc acatcgcaag ctgcgtttct aataacatat tcagaaacag
    11041 aaccaataaa taaacgttct acagcattca atcctgtggc tcctaacata atcaaatcta
    11101 cttgattatc ttctgggatt tctctggcaa taatttgttt tggtgagcca tattctacga
    11161 cagaggttac attattcaag ccagctttct tcgcattgct ttcataatct gccaatgttt
    11221 gctttgccat ttcagtagct tgttctgcaa gcattccatc aaaggatgaa acggtttgga
    11281 aagaacgtgt gtcaatgaca tgtgctaata gaagttctcc gttatttcgg atagctacat
    11341 tgaccgcctt tttaaaagct aattcagctt catcagaacc atctacggca accatgattt
    11401 ttttgtattg ttgtaacata agcattcact cctcaagtca atcgtattgt actcttttat
    11461 tgtagaacac tttagggaaa aagagaatta tttactgctt tggttcagca attttttttg
    11521 caaatcggat actgaaggtg gcagaaacaa taattacgat cagtaaaaag accaaggcaa
    11581 taaagcgttg attgaaaaaa gctgttaaaa tacccattac gcctaaaaca gcatatgcag
    11641 caccgtaaaa ttgtaggaat ccttgatttt tatcattttt gttgatcaat acaaaaaaga
    11701 tatcctgttt attaaataaa taatagctaa cgaataataa tactaaagtg gcgataatca
    11761 ttaaaatcct aatcatgaac aaatcctcca aaataagcaa caaatagaaa aagcaatttg
    11821 ccaagagcaa attgctttat tctttaaaag atggtaatct ccgttaatgc cgttgtttgt
    11881 cctgtagtat tgatcccgcg aattcaagca ggtgggtccc atggtcgtag cttcgaacta
    11941 ccaaatgata aggcatgtta ccagcagtga cacaagacgg gtcccaagat atcaataaaa
    12001 atgttcggtc aacacacaaa gaggacaatt cgaacatcac agatttccca cctctatagt
    12061 agcatatgaa tttaaaaaga gcaatgagag agctcgcaga aacagaaccg ttttcttgtc
    12121 tagttttttg gaggatgctt tttccactct tttatacggt aatactgttg acccagagcc
    12181 tgttcatatt tccctgtatc ttttggctga taatactgag cattcttcag tttatctggt
    12241 aaatattgct gattgaccca agcctgatca aaatgatgag gatactgata acccacgcca
    12301 cggttgagtg atttcgcacc tttgtaatgg ctatctctca aatgatcagg aacatctcca
    12361 gctttgccct ctcgaatatc agcaagtgct gcatctaatg ccatgtaggc agaattggat
    12421 tttggcgaca agcaaagatc gacgacgacg tctgctagtg ggatacgtgc ctcaggcaat
    12481 cccaattttt cagcagctaa tacagcgttc actgtacgag ccgcagcagc aggattacct
    12541 aaacctatat cttcataacc gataaccatc agacgccggc aaatacttgc cagatcacct
    12601 gcctcaacta aacgggcgag ataatgaagt gcagcatcca catcacttcc acgaatcgat
    12661 ttttgaaaag ccgaaataac gtcataatga gcatctccat ttttgtcatg ggtaagagct
    12721 tttctttgga cacattcttc aatgatcgac agtgtaagat gaatccttcc ttctttatct
    12781 gaaggagtcg atagtgtagc cagttccagc ccattcaatg cactccgcaa atcgccgttc
    12841 gttgcacgcg aaaggtgaag aagagcatct tcgtctaact ggattgcttg ttgtccaagg
    12901 ccgcgttctt tatcccttaa agcatgttcg actgccaact ggatgtcttg ttctgttaat
    12961 ggtttgactt caaagatctg tgtacggctg cggatcgctg gattgatggt aatataagga
    13021 ttctcagtag tcgctccgat caagatgatt cggccacttt ctaaatgagg aagcagaaaa
    13081 tcctgttttg ttttatctaa tcggtgtact tcatccaata agaggatcac tgtaccactc
    13141 atttttgctt cttccgctac aacttgtaga tccttttttg aatctgtagc ggcgtttagc
    13201 atgcgaaatg cataattagt agagccggcg atcgcacttg cgatactcgt ttttccagta
    13261 ccaggaggtc catatagaat catggaagag agcatgcgag catcgaccat gcggcgtatg
    13321 atttttcctt ccccgactag atgctgctga ccaacgactt catcgatcgt tcgaggtctc
    13381 atgcgataag ccaatggctg ttgcatagga ttttcctcct tcttgttttt acgtccctta
    13441 attatatcat atttagcaag atgcctagaa agaacagcct tctgttgttt tgttttcata
    13501 gcttatccaa ctaatattgt gtatgataaa gtaggtgaga aaaatgacgg ataaacaaaa
    13561 gatagatgaa tttttttgga ataaaacgac gccagaagtg gcacgggatt tattggggat
    13621 gtatttggag catgacacac cagaaggaag attagctgga tatattgtag acgcggaagc
    13681 atacctaggt ccagaagacg aggccgccca tagctatggt ttaagaagaa caccgcgtgt
    13741 gcgggcaatg tatgaaaaac caggtacgat ctacctttac acgatgcata ctcatcggat
    13801 tttgaacatc attactcagc cagaagggat tcctcaagga gtaatgatac gtgcaatcga
    13861 acccgccgca atgattgatc aaatgagtaa aaatcgtggc ggaaaaactg gtccagatat
    13921 cagcaacggt ccgggaaaat tagtggaagc attagcaatt cctcaagaac tttatggtca
    13981 gtctatagca gatagttcgt tacgcttagt ttttgagaaa aaaaagacac caaaaaagat
    14041 caatgctttg cctagaatcg gtatcccaaa caaaggtgta tggacagaaa agccgttgcg
    14101 ttttgttgtg tcgggaaatc cttatatctc tttacaaaga aaaaaccaaa tcgagaaaga
    14161 ttggggttgg aggaaagaaa atgaaaaaga aggatctatt aacatattta gatgaaataa
    14221 ttgaaaaaca agtgacagac tatgatgtag ctttagattg ggatacaaaa aatcatacga
    14281 tcgagatcgt tgttcgcttg tttgcagaaa ataaagcaca atttgagatt gatgatgcag
    14341 aagggatcgt ttcgcaagaa ccaattatcg aatttgaaga cggcgtactt ctttttaatc
    14401 ctcagaaatc tgtttttgat gaacgcaatt atctagcagt aatcccttat gaaggaaaaa
    14461 aagggttggc aaaatcagta gctgattctt tagtggaata tctaaatgaa gtattagctc
    14521 aagggcaaag cgatttgctt gattttttgg atgaagacga tgacgaagct gtattcgagc
    14581 ttcactggga taatcaacaa ctagcagaat tagtagaagt aaaacagcaa aatgaagcag
    14641 atacttattt accgtaccca agttattaag gagtgacaga tatgaaatgg aatgaagtaa
    14701 aagtagaaac tgaaagtgaa gcagtcgaag cagttgctaa tattttgatg gaggcagggg
    14761 cgagtggtgt cgcgattgaa gattctttag atgttgaaca ttttcagtcg gatcaatatg
    14821 gtgagatttt agataaaaat gattttacat cgctgaaaga aggcgcacta gtcatggctt
    14881 attttccgga aacgactttc ttaccggaaa tcttgccttt tatcaaagat cgtatcacac
    14941 aattacctga gtttggtcta gctattggaa aaaataaagt aacagttagt gaagtagagg
    15001 aaagcaactg ggcaacagcc tggaagaaat actatcatcc ggtgcgtatt acgcgaatgc
    15061 tgacggttgc gccaagctgg gaaaactacc agacagaaga tcctctagaa aaaattatca
    15121 cactagatcc tggtatggca tttggcacag gcactcatcc cacgacttcc ttgacattga
    15181 cagcactaga aacagtcttg cgtggtggtg aaacattgct agatgtggga actgggtcgg
    15241 gcgtgctaag tattgcagca aaatatcttg gtgcaaaaga tgtctatgcc tttgatttag
    15301 acgaagtagc cgttcgttcg gcgaaagaaa atatggatat gaatgaaata gccaaagatg
    15361 tccatgtatc agctaacgat cttttgacag gaatcgagat tgaaagtgat gtaatcgttg
    15421 cgaacatttt agcagatatc atcttattga tgatcccaga tgcttggcgt ttactgaagc
    15481 aaacaggcac gctgatcgtt tcaggaatca ttgaagagaa gaagcaaatg gttctggatg
    15541 ccatgttaga acaagggttt atcgtggatc aaattttcca gcaaaaagac tggtatgcaa
    15601 ttattttgaa aaaaccagag gtggaataga tgcagcggta ttttttaaaa gaatcatatg
    15661 aatcaaaaga ctattttgtt ttagaagggg aaaactatca ccacattgta cgggttatga
    15721 ggatggaacc cgaggatgaa gtttttttgg cattcaatga tcaaatagcc attgttgcag
    15781 aaatcacgga gattactgaa gaggctgttt atctgaaaga aattaaaaaa gaagaaaatc
    15841 aaaaggaact gccaagttcg attaccatag cgagtggcta tcctaaaggg gaaaagctgg
    15901 attggatcgt tcagaaatcc actgaattag gagcgcaagc ttttcttggt ttcccggcat
    15961 cagcctctgt cgttaaatgg gatgcaaaaa aacgtatgaa acgacaacaa cggcttgaaa
    16021 agattgctca agaggcagcg gaacaatccc atcggcagat cgtgccatca gtagagttat
    16081 tggaaacgaa acaagattta tttgagcggc tagatgatta tgacgtggtc ttggttgctt
    16141 atgaggaatc agcaaagcaa ggcgaaaaat caaatgcagc caaaattctt agtgaaacaa
    16201 aaccagatag tcgaatctta gctgtctttg gaccagaagg tggattatcc cgcgatgaag
    16261 tcgattattt tgtagaacac aaagggatac tttgtggatt aggaccaaga atccttcgta
    16321 cagaaacagc acctctttat ttattaagtg ctgctagcta tcaatgggaa ttaatgaatc
    16381 cttctttcag ctaacgttct tcattaataa aagagtatcg acagaaagaa ttgaaaaaat
    16441 agtcaacaat ttgtataata gaaacaatta tttatacaat ggatacgaaa aatcgtttga
    16501 aaaagctttt aaaaagcgaa catttccgta aaaaaacgta taactgcacg gattttggga
    16561 caattatcaa acagatattt gtcccgattc ccgttttacc gaaaaaagaa agggggatcg
    16621 attcctgtga caaaagaaga aattttaacg ggacccggtg tcataaagct cgttagtcaa
    16681 tacatgggtc ccgaacatgt agcatttgtt gagaaagcat gcgaatacgc gactgctgca
    16741 catgacggac agtttagaaa atcaggcgaa ccctacatta ttcatcctat ccaagtcgca
    16801 ggtatattag cagatttaaa aatggatccc catacagtgg ctacaggctt cttacatgat
    16861 gttgttgaag atacagaaat cactttagaa gatctgagag aagaatttgg cgatgacgtt
    16921 gctatgttag tggacggcgt aaccaaatta gggaaaataa aatataaatc ccacgaagag
    16981 cagctagcag aaaaccaccg aaagatgctg ctagcaatgg ctcaagattt acgagttatc
    17041 atggtcaaat tagctgatag actacataac atgcgtacgt tgaagcacct gcgagaagat
    17101 aagcaaagaa ggatcgctca ggaaacttta gaaatctatg cgcctcttgc acatcgtcta
    17161 gggatcagcc ggatcaaatg ggaattagaa gatacagcac ttcgttatct aaatccaaaa
    17221 cagtattacc gtatcgtcca cttgatgcag acgaagagag aagaacgtga aaaatacgta
    17281 agtggtactg ttgaagatat tcgaatagcg acagaagagc tggggatttt tgcagaaatc
    17341 tatggacggc caaaacacat ttattcaatc tatcgtaaaa tgaaagatca gaaaaagcag
    17401 ttcaacgaaa tttatgactt gctagctatt cgagtgatcg tagattcaat caaggattgt
    17461 tatgctgtat taggagcaat ccacacaaaa tggaaaccaa tgcccggcag atttaaggat
    17521 tatattgcca tgccaaaagc taatatgtac caatctttgc atactaccgt gatcggtccg
    17581 gcaggaaatc cggtagaaat tcaaatccga acacaggaaa tgcatgaaat cgctgaattc
    17641 ggggttgctg cacactgggc ctataaagaa ggaaaaaatg aaaaagtaga accagatggt
    17701 atgacgaaac aattaagctg gttccatgag atactcgaac ttcaagacga aagctatgat
    17761 gcttctgaat ttatggaagg cgtaaaagga gatatcttta gtgataaagt ctacgtcttc
    17821 acaccaaaag gagacgttac tgagttacca aaaggatccg gaccattgga ctttgcatac
    17881 agtatccata cagatatcgg taacaaaacc actggtgcaa aagtaaatgg caaaatggtg
    17941 cagcttgatt acaaattgaa aaacggagat atcattgaga tcatgacttc tccaaattca
    18001 tttggcccaa gtcgcgactg gttgaaatta gttgctacta gcaaagcaag aaataagatc
    18061 aaacgtttct tcaaagccca agatcgagaa gaaaatgtga tcaaaggcca cgaatccgtg
    18121 gtcaaatgta ttacagatct aggatttacg cctaaagata ttttgacgaa gaacaaactg
    18181 caagaagcac tcgatcgttt taattatcaa acagaagatg atctctatgc agctgtaggg
    18241 tatggagaag ttagcccctt gacgatggcc aatcgtctga ctgaaaaaga acgtaaagaa
    18301 caaaaaatcg agcagcaaaa gcaagaagca gaagaaatca tgaatcagcc gaaaaaagaa
    18361 cctgacaaaa tgaaagtacg tcatgaaggt ggcgttgtca ttcaaggggt agaaaactta
    18421 ctaattcgta tcagtcgctg ctgtaatccg attcctggtg atgatatcgt tggttatatc
    18481 actaaaggca gagggatatc cattcatcgt cgagattgtc cgaatgttca gcctgacaaa
    18541 ccaaatgtag cagaacgttt gattgaagtc gaatgggaag atacatcgaa tacacgaaaa
    18601 gagtatgatg cagatttgga aatttacggc tataatcgtt caggcttatt gaatgatgta
    18661 cttcaaacag tcaatgcgct aacgaaaaat ctcaacagcg ttgaagcacg gacgaataaa
    18721 gataaaatgg cgacgatcca tttgacggtt ggtatccaga atttatccca cctaaagagt
    18781 atcgtggata aaatcaaagc agtacctgat gtctacagtg tacgccggac gaatggatag
    18841 gaggacagat atgagagcag ttgttcaacg ggtaagtaaa gctgctgtgt cagtcgatca
    18901 gcaagaaatt ggaaaaattg gctacggcct acttgtttta ttaggcattc atgaaaccga
    18961 tacgcaaaaa gatgtggact atctggtaaa aaaaattgca caaatgcgta tttttgaaga
    19021 tgaacaagga aaaatgaata ttagtgtaga agatgtggga ggacaaattt taagtatctc
    19081 tcaattcaca ctgtttgcgg atacgaaaaa aggaaatcga ccaagctttg tggaagccgc
    19141 acgaccagaa acagctattc ctttatacga atcattcaat gaaggaatca gaagtcgcgg
    19201 catcacagtc gctaccggac aattcggagc agatatgtcg gtttctctcg tcaatgatgg
    19261 acctgtaacg atcattattg attctaaaaa caaataaagt aatctggttt tcactagtaa
    19321 aaactatgaa acacgtcgct taagttagac tgataaactc taacttaagt ggcttttttg
    19381 tctgcaaaaa gcgcttcttt tgtcagcagt cggaaaaaaa gttaagctta aattatacaa
    19441 gttatttttt ataggaggtt gctgaagatg aaagaagtac cagtagtaga tatcaaaatc
    19501 acagatttaa aaaaattgta tcaaaaagga tacaacatgt tggaggagtt acgccatgaa
    19561 gcggacgctc ccgtcgtcaa agcaaagatt ttcaataaag aagctatcac gatctacgga
    19621 tcatcagcag caaaagtatt ttatgatcca aggaatttca aacgaaaagg tgctatgcca
    19681 aaattagtat tgaagacact ttttggtcaa ggtggagtac agactttgga tggagctgct
    19741 catcaccatc ggaaaaatat ttttatggat ctaatgacac cggaaagaat ggaagattat
    19801 catcgtatct tagataagaa tctgacacaa gcacttgaag cacagcatgg tcaatttgaa
    19861 ctatttgatt taagtaagat ggttttcttt acatctattt gcgagtgggc aggtatcaat
    19921 ttatcagcga tttctaaaga tgaggtcgaa aaattagcag aatatcaaat ctctatgatc
    19981 agcggaacgt ttacttctcc gattgatcac ataaaaggtg tcgagaatcg caaaaaatca
    20041 gaaaaatggg cgcaaggctt gattgaagaa gctcgacaaa atcctgtggc tggaaaagaa
    20101 aatgtggcat tatatgcttt tgccaatgcc acagatttag atggtcagct tttacctctg
    20161 gaagtggcag ctgttgaatt attgaacatc atccgtccaa cggttgctct aactgtgtgg
    20221 gcggcattga tgggacatgc acttttcagt agaccagatt tgtatcagca attaaaaaat
    20281 gacttttcga cgttacaaga tccgtttatt caagaaatga gacgctatta tcctttcttc
    20341 ccaatgctac ctgcgatttc gctgaaagaa gtagaagtag atggttatcg tataccagaa
    20401 ggcagttggg ttattttaga tttatatgga acagaccatg atgaacgaac agttgaagcg
    20461 ccagattcct ttatgatcaa acggtatgta gggaaagcaa aagatatttc ctataaagaa
    20521 gaatatgaga tgattgcaca aggcggggga aattttcgac aaatgcatcg ctgtgcagga
    20581 gaatggatca ctcttcatag tctacgtgtg ttttccgatc agctagtaaa taagtttgag
    20641 ttctctgtac ctgaacaaga ttggacgatt ccattcaatc agtttccgac ttatccgaat
    20701 agccgagcgc tactttacaa gaattagatt agcataaact tatcgaaaaa aaggaagatc
    20761 aaacagtgct gatcttcctt ttttcatatt actataaatt gataaggtca attgatcaaa
    20821 atccacgaaa tgcttcatca atggtttgat aatcttcatt ggataaatgc aaatctaaag
    20881 cttggacatt gtcagatact tgctctggac gcttagctcc aggtataact acagacactc
    20941 gtggattctt catgtaccaa gctaaaacaa gttgcgcagg agtggcttgg tatcttttag
    21001 caatggggcg aagtacatcg acagcggtta aaatttctct gaaacgttct ccttggaaat
    21061 ctggatttct ttttcgagga tctccttcac caaattgttt ttcctcacca agttcatatt
    21121 ttcctgtcag caaaccggaa gcaagaggaa aatagggaac aaaactgatt ttgttttttt
    21181 caagataagg gaataattct ttttcagcct gccgatggat caaactgtat ttatcttcta
    21241 ctacatcgac atatccatct gcattcgctt ctttcaattg ttctaacgtg aagtttgaca
    21301 caccgactgc tcggattttt cctgcttctt ttaattcgtg caatgtagca actgattcat
    21361 tttttggtgt gctttcatcc ggaaagtgga tataaaaaat gtcgatgtaa tcagtttgca
    21421 gacgttttag tgcatcttcc accgattgtt tgagaaactc aggagaatta tcaaatgttc
    21481 gccctttatt tggtacatga gcagcttttg tagcgatgac gacacgtgag cgatcatatt
    21541 ctttttcctt cagtacctcg ccaatcagtt cttcggaacg gccgtttccg taagcataag
    21601 cggtatcgat caaattgatc ccgctattca aagctgtacg aactacttgt tttcctgtct
    21661 catcttccaa accgtcaaaa agattatgtc ctccaacggc atttgctcct aatccaagct
    21721 gttctgcgta gacttgtgaa tgtccgattc ttatttctgc catagctgat tcctcctttt
    21781 tgtctagtct aaaacaaaat accaaaaagc tgaactaaaa cgtttttgtg aggggaaata
    21841 gtcagatatc tcttgacttt gagactggtt tttagtaggt tataagtaat atcgaatgtc
    21901 aatgaaagag cgagtaagtt tttgaaactt tacaagagag gatcatcgag gctgaaaatg
    21961 atctaaagtt tcattagcct aaagacactc tggagatgga cgagtgaaaa gccgtcccgc
    22021 gtacgagcgt tattcgtaag aggaaagaga aatctttcaa aattaggtgg taccgtgtat
    22081 gaatttcatt cacccttgtt catgatgaac aagggtgatt tttttattta catagctaag
    22141 aaagggagag aaaactatga gtttccaaag accaaaagga acaaatgata ttttgccagg
    22201 cgaatcagaa aaatggcagt ttgtcgaaga aacagcacgt ttattattca gagattatca
    22261 gtataatgaa atgcgtacac cgatttttga acactatgaa gtgatatccc gaagtgtagg
    22321 ggatacaacg gatatcgttt caaaagagat gtatgatttt tatgacaaag gggaacgcca
    22381 tgtgacgctt cgaccagaag gtacagcacc aatcgttcgt tcttttgttg agcataaatt
    22441 gttcggccca gagtatgcga aaccgttcaa aactttttat atgggaccaa tgtttcgcta
    22501 cgaacgccct caaaaaggac ggttacgtca attccatcaa atcggagtag aagcatttgg
    22561 atcagaaaac cctgcaactg atgtggagag catggtgatg gcacttgatt tcttcaaaca
    22621 gttggggatc acgcaaatcc ggctagtcat caattcatta ggtgataaag aaacgcgtca
    22681 agcctatcgt caagcattga tcgactatct tttacctttt gaatcagagc tgagtgaaga
    22741 ctctaaacgc cgccttcatg aaaatccatt acgcgtattg gacagtaaag acaaacgaga
    22801 tcaaaaattt gtggcagaag ctccatctat cttagattat ctaagcgagc ctgctcaaaa
    22861 acattttgat accgttgttt ccatgctgga tgcattggct gttccttatg aaatcgacag
    22921 taatatggtt cgcggactag attattacac acatacgatt tttgaaatca tgagtgacgc
    22981 accaaaaatg ggggcacaag caacgatttg cgcaggcgga cgttatgaca atctagtgga
    23041 agaactaggt ggtccttcaa cacctggctt cggatttgca atgggtatcg agcgattact
    23101 cttgactatg gaggcagagg aagtagttat ccctgcattt aatgagttgg atgcttacgt
    23161 ggtcgcactc ggagacgaaa caaatatcga agcattgaaa gtggtccaag cgattcgaaa
    23221 ctttggtttt tcagctgacc gcgacttcat gaaccgtaaa gcaaaagcgc aatttaagac
    23281 agcagataaa gcgaatgcca aattagtatt ggtgatcggt gaagatgagc ttgctagtca
    23341 aacgattaat gtcaaagcaa tggcaagccg aaaagaaaaa agttttccat tagaagagat
    23401 atatgaacat tttgatgaag tctatgatga aatgacttta ttcgagtagg aggaaaaaaa
    23461 taaatggcac aaagaacgat ctattgcgga ttagtttccg aacaacaatt agaacaaaca
    23521 gttacattaa aaggatgggt acaaaaaaga cgagaccttg ggggagtaat ctttatcgac
    23581 ctacgtgatc gtgaaggaat cgttcaaatc gtctttaatc ctgaaatcaa caaagaagca
    23641 tgggaaatcg cggataaatg tcgcagtgaa tacgtgatcg aagtgactgg ggaagttaga
    23701 agacgcggag aaaatgcagt aaatcctaaa atggccactg gtgaattcga agtgattgca
    23761 agtgaaatcg tgattttgaa tcaagcaaaa accccgccat ttcaaattga agatgatcaa
    23821 gcaatcagtg acgaaatacg tttgaaatat cgttatttgg atctgcgtcg tccaaaaatg
    23881 actaaaaaca tcaaattaag aaatgcgaca acaaaagcga ttcgtcatta tttagatgat
    23941 aatgactttc tcgatatcga aacaccttac ctagggaaat ctacaccaga aggcgcaaga
    24001 gactacctag ttccttcacg tgtccatgct ggacatttct atgcgttgcc gcaatctcct
    24061 caattgttta aacaattgct gatgaatgct ggattcgacc gttattatca aatcgttcgt
    24121 tgtttccgcg atgaagattt acgtggagac cgtcagcctg aatttacgca agtagatatt
    24181 gaaacaacgt tcttaacagc agaagaaatc caaacttaca cagagggatt gattgcgaag
    24241 gtaatgaaag aagttcgcgg tatcgaagtc acattgccat ttcctcgtat gacctatgat
    24301 gaagcaatgg ctcgctacgg cagcgacaaa ccggatacac gttttgatat ggaattgatc
    24361 gatctaagcg atacagtcaa agaagtagaa ttcaaagtct tccaaatggc tcttgaaaac
    24421 ggcggtgtcg tgaaagcatt gaatgctaaa ggcgctgcag atcgctactc acgaaaagac
    24481 atggatcagt taggtcaata tgtaggacaa ttcggagcta aggggcttgc atggctgaaa
    24541 gtagaagaag acggattgaa aggtccaatt gctaaattca tgggagaagc aacagaagca
    24601 atcattaaag caacagatgc aaaaccaggt gacttgctga tgtttggcgc agataaaagt
    24661 gaaatcgtcg ctgctgcatt aggtgctatc cggacacgtt taggaaaaga attaggacta
    24721 attgatgaat cgaaattcaa tttcttatgg gtaactgatt ggccacaatt tgaattttca
    24781 gaagaagaag gtcgatatgt ctctgctcac catccattca cgatgccaaa agaagaagat
    24841 atcccgttat tggctgaaga ccctgcaaaa gtctatgcag aagcttatga tatcgtcttg
    24901 aacggttatg aattaggcgg aggttctatc cgtattcata ctcgtgagct tcaagaaaaa
    24961 gtactagaaa ctctaggctt tacaaaagaa caaatggaag aacaatttgg tttcttgtta
    25021 acagctttag attacggatt ccctccacat ggcggtattg cattaggatt ggaccgtttt
    25081 gcaatgttat tagccggaga agataatatt cgtgaagtca ttgcctttcc taaaaacgga
    25141 aaagcagcag atgtgatgag tgatgcacca agtatggttt cgcctttaca attgttcgaa
    25201 ttgaatattg atgtaacgaa tatagaagaa tagccgataa aaaggtaaaa aggatttgtc
    25261 gaatacgctt cttttgacaa ccttgattta attgaaaaac actttataat gaagccataa
    25321 catataaagc ggggcttttc catgaaaaaa acattcgaaa attgggctta tcatgattac
    25381 acgaccaagc tttcggtatc tatcgtttat gcgttgatcg catcagtcgc attgaatttc
    25441 ttttatcagc caggacatat ttattcaagt ggtattactg gtctagctca gattttgacg
    25501 actttgtcaa aatcactgat aggattcgaa gtaccggtat caacaacttt gtatttactg
    25561 aatatacctt tgtttgtatt ggcgtggtta aaaatcggga agaagtttac aattttcact
    25621 tttatcacag tcactttgac ctccctttgt atccatattt taccggaaac agtcctatct
    25681 accgatccaa tcatctgtgc gttgtttggg ggggctgtga tgggactagg tattgggcta
    25741 gcgttgagaa acggcctatc atcgggcggt ctagatatcg tgagtatcac gattcgtaaa
    25801 aaaacaggaa agtcgattgg ttctatttcg atctacttca atgcattgat cgtttttgtt
    25861 gccggaattc tatttggctg gcaatatatg ttttacagta cgttgtctat tttcgtcagt
    25921 ggaaaagtaa ctgatgtcgt ttatactaaa caaaagaaga tgcaggtaat gatcgtaacg
    25981 aaacatccag aaaaagtagt aaatgaaatc caaaactgta tgcgtagagg gatcacgatc
    26041 atcaatgaag cagaaggtgc ttataagcat gataagcaga cagtattgat gacgatcgtt
    26101 acacgatacg aactgcctct acttagagaa gcaatgaaag cttctgatcc acaagctttt
    26161 gtaagtattg cagaaaacgt ccagatactt ggtcgtttct atgaggatgg catttagatc
    26221 gagtttgaca aaaattcatg aaatactgct gtagacagac gttcgtttgt ctgcagtttt
    26281 tttctaccat agtagaatca gcaaagaaaa actgcttttt tatttgaaat tttcttaaag
    26341 ttgaaaaaaa gcatgtttat ttttttaaat ttatgggatg atagaaaaaa atagtagaga
    26401 aaggacacga ttctacttgc tattttcact taatacccgt atattacgaa tgaaaaaagt
    26461 ttatgaaaag ttggaatatt gaatggaaaa acaaaaaagg ttaaaaaaca gccgctacat
    26521 cactgggttt gatgggctcc gttcattagc tgtggtgggt gttatcttct atcaccttct
    26581 gccaactagt atgaaaggcg gctatttggg cgttccgatt tttttcgtcg tatctggtta
    26641 tttgatcacg gatctgctgc ggcaggaatg ggaacaaaat ggaaaaatca atatttggca
    26701 attttatgtc agaagaatga agagactcta tccggcactt gtttttatgc ttgtgacagc
    26761 ttcggcttat atcactttgt ttcagcgagg gctgttaaac aatcttcgag gagttgtggt
    26821 cagcagctta ctgtatgtca acaactggtg gcaaatcaat aacggtttat cttatttcga
    26881 tcgatttgct aatgagtcac cgtttacgca tatctggtct ttagcagtag aaggtcaaaa
    26941 ctatttggtt tggccgatct tgtttgtatt gttgatggta tttgttcgta aaagaaaatg
    27001 gatcttttat actgtgttag gtacatcctt cctctccgcc atcttgatga tgatcttgta
    27061 tacaccagga ggcgatccaa cccgtgttta ttacggtacc gacactcgtc tgttttctat
    27121 atggttggga agtgcgcttg cgtttgtttg gccaagtacg cgtttaaaga aaaacatccc
    27181 gaaacaagcg aaaaatattc tgaatatcgc tggaggaatc tcgcttttgg cactgattct
    27241 ttgcttcttc tttttagatg atcattacaa tttcatctat tacggaggaa tgctcctagt
    27301 gagtctgtta tgcgtagtgc tagttgcagt gacagcacac cccggagcaa gtttgaatcg
    27361 atggctgaca aatccagtat ttacttggat tggcaaacga agctatggca tttatctgta
    27421 tcagtttcca gttatgattt tttatgaagc aaaggtgact gatattgccg atcatgtttt
    27481 gttgcatact ttagtggaaa tcacattgat tctaatcatc agtgaacttt cttatcgata
    27541 tgtggagcgt ccgctagcac gttttgatta tggtcgtaca ttccaagtga tgaaaggatg
    27601 gttcagcaaa ccgatcatca gtaagagcaa accatgggtt gttccaggca cattacttgt
    27661 agcaattgca ctcgttggtt tcgttacggc accgaaaaac gctgtaacag ccgatcaaaa
    27721 acaactgcag gcaaaaatcg ctgaaagtaa aaaattagca gaagaaacca gaaacaattc
    27781 agaaaacacc actagttcgg ttgaccaagc gatccttgat aagtacgagt tgtccaaggc
    27841 acaagggaat aaggcacaaa aattagaact aacggcattt ggtgattcag ttatgttgga
    27901 tgctgcaacg gatctacaag aactttatcc aaaagtagtg gtagatggag atgtcggacg
    27961 acaattatat gccagtgtac cgtacattga aaaattaaag gaacaagatt tgttgaaaga
    28021 cactgttctg attggactag gaacgaatgg ctcattctcg gaagctcagt ttgatagtgt
    28081 gatggatgca atcggtgatc gaaaagtata ctgggtcaat gtccgagtac cgacgaagcg
    28141 ctggcaaaat gacgtgaatg cgatgcttga acagatgacg aaaaaatacg acaatatgac
    28201 gctgattgat tggtacagct acagtaatga ccatagtgac tggttttatg atgatcaagt
    28261 ccatccaaat cctgaaggga tgacacatta cattcactta gtcagcgaag ccttgctagg
    28321 aggaagttca agtacgaaca gcagtgagac aaacgtgaca gaatgatagc tacaaaaaac
    28381 ttcaggcaaa aacatttgtt tttgcctgaa gtttttttat atttaccgtg tttttaacat
    28441 tcatatagat tcaggaagca aaacggttga gtgtgtctgt aaataacgtt ctttttgttt
    28501 tcgggtgagt tttttgaatg cagcttctgc ttttgttgct tcgcttcttg aagaaaatac
    28561 ttctgcatgg atcatcttaa caggtcttct tttttgaaga cgagtatatt tagcaccagt
    28621 accattgtta tgttcctcta atcgtctaga aagatcggtt gtatatccac cataaaatgt
    28681 ctgatcttta cagaataata cgtaaaaata atgttcattt gccatagagc atctcacgaa
    28741 tctctgcagt gtattctcca tccatattgt aagtgatcaa tggtggagcg actttgaatc
    28801 cgtctaattt gccttctttg attccttcaa ctaacaaaat atttgcctct tttccaggtt
    28861 ttgggtaaac aaattggatc cttttaggtg caattcgata gtcttccatg acctgaatga
    28921 tttctaagaa ccgatcagga cgatggacca tagtaaagcg tccatttgtt tttagtagct
    28981 tacttgaaat atcgatcact tcttctaacg tcgtatgaat ctcatgacgg gcgattgcca
    29041 ggtgtttatt agggttcgtt ttatttgttg gcagcccttt gaaataaggt ggattacaga
    29101 cgactaagtc acaagaatta tgtcgaacga cagataagct gtctttcaga tcgatggtgt
    29161 ggacagtcat ttgttcattt aaatggttaa gcgcgatact acgtcttgcc atgtctgcta
    29221 gacgaggttg aagttcgata gcgtcgatag ccgcctgggt tcgtttactt aaaaaaagtc
    29281 caactgcgcc atttcctgca caaaggtcaa caatcttccc ttttttaggt acacgggaaa
    29341 agtgagcaag cagcacagca tctaaagaaa aagaaaacac tttggaactt tggataatct
    29401 tgatatcttc tgcgtataat tggtcgattc gttcgtcttc gtgaagcatg gattccctcc
    29461 aaaatgaaaa aaatagtcgt ctgtattata ctatttttat taaaaagaac caaggtggct
    29521 tgtcaaacct ctatttttta tgcatactat gaccataaga agggaaagga agagactatg
    29581 ttttatcgat ttatgcgcgg ggtagttcga gtactattag ccttagttaa tggaaatgcc
    29641 cgctatgaga ataaagaagt tttaccgcaa gatgagaatt acatactagt tgctccccat
    29701 cgtacttggt gggacccatt atatctggcg gtagctgcag cacccaagga attcagcttt
    29761 atggcaaaag aagaactgtt caaaaatcca gttctgcgat ttatattaac tcgatccaat
    29821 gcttttccag ttaaaagaga aaatccagga ccaagcgtca ttaaaacacc tgtaaagatt
    29881 ttgaaatcga caaatttgag tttgatcatg tttcccagtg ggacaagaca tgctacagaa
    29941 cttaaaggcg gaatggcatt gatttccaga atgtctaaag cgaaaatcgt gccagctgta
    30001 tatcaaggtc cgttgacact tggggatctg ttcaagcgtc agagagtgac agtgcgtttc
    30061 ggcgaaccaa tcgacttatc agatatcaag aaaacggata aagaaggtat ggcggagatc
    30121 gaacgacgaa cacaagcagc ttttgacaaa ttggacaaag aagtgaaccc tgatttcaaa
    30181 tatgaagtca aaaaaaaata aaatgataag tttatgcgtg ggcggtcttt tgaatctact
    30241 gtcctgttca gttcagactt tttcaatgaa aacgagggag agacgtcaag tctgccctct
    30301 ttttgcgttc caatatgtaa aatagaagac aggtgtcatg taatggatca aagtgttata
    30361 ataattagaa ggacattttt tgaggtgatt gaatgcgttt tttacataca gccgattggc
    30421 atatcggaaa aaaactacaa ggttatgact tgattgaaga ccagcgtcat gtactatctt
    30481 ctatactaga tatcgcgaaa gaagaaaaag tagaggcaat cgtgattgcc ggagacttat
    30541 atgataggag tgtcccagct gttgatgccg tagaattata caatgaattg atggtcgatt
    30601 ggaatttaaa ggaaaagatc ccgatttttg ctatatcagg aaatcacgat agcagtacga
    30661 gactttcagc gggtacgcca tggttcagtt attcgaacta ttttttgcga acacagctag
    30721 cagaagcatt tgaaccagtc gagtttaaaa acgtacaatt tttctttctt ccttactttg
    30781 aaccaattgc agcgagatta tattttgaag atgacaatat ccgaacgatc aagcaagcgg
    30841 tagccaaggt gatagataaa atgcaggagc tatttgattc agaaaaaaaa caagtattga
    30901 ttagccattt ttttgtaatg ggaagtgtaa aatctgattc ggaaacgaaa atcgaagtag
    30961 gcgggttgga ttcgatccct ggtgaactac ttacaccgtt tgattatgta gcactaggac
    31021 atttgcataa tcaagctgct ttgagacagg ataatgctcg ctatagcggt tccccattga
    31081 aattctcttt atctgagatc aaccaaaaaa aaggcgtatg gatcgttgac ctgacaacag
    31141 gcctggagtt gaattttagg gagatcgaac ccctgcatga tattgaagaa ataacagcga
    31201 gtttcaaaga attgctggat cctgcctttt atcagtccgt caatcgggag aattacatct
    31261 atatctatct cactgataga acgatcatcc cgaatatgat gaaccagctt cggcaaatat
    31321 atcctcgtat tttggggatt gaacgggcaa acggtcgagc agaacaaaca aagcgcaaac
    31381 gaaaagtact agaaatgaaa gatcctcaaa aattagcaga agcatttttt agtgaaatga
    31441 cggaagagca gctaactgca aaccaagaaa aatggctcgg agaaacgttg caagaaatca
    31501 tggaaatgga atgatcaaga atgaaaccga aaaaattaca acttaaaaat tttggtcctt
    31561 ttatcaatga gaccgtagat ttttcacgtt tgacagaagc accgcttttt ttgatcagcg
    31621 ggaagacagg agctggcaaa acgacgattt ttgatgggat caccttcgca ctttttggtg
    31681 aaacttccgg aagacttcgc tctggaaaag agatgcgttc tttgtttgct acaccagacg
    31741 aagaaacaag cgtgactttt tcttttgaac atcagcagat gagctatgaa atcgaacgaa
    31801 aaccagaaca agttttagca aaacgaaaag gaaacggaac aaaaaaacag gcagccaaag
    31861 taacgttgac catttttgat gggaaaggaa aagaaataaa acaaatcaca aaacgtacag
    31921 aagcagatca gttgatcaga gaactgatga atctcgatgc gaaacagttt tctcaaatcg
    31981 ttttactacc ccaaggagaa ttccgtaatt ttttggtttc atcaagcagt gataaagaga
    32041 ctgttttacg tcatttattc ggtactcaat ttttccaaca gttcaatgag cgattaaaag
    32101 aaaaagcaaa acaacagcaa caaaaattgg atcatttaga gcaagagctg catcttctgc
    32161 aaaaaaggtt tgttttagtc aaagaagagg aaccagcagc agggtttgaa gccgttttag
    32221 cccaatggac aaaccatcag gcaatactcg aaaaacaaat cacggaagaa aaagaagctc
    32281 taacggaact tcaggaaaaa caaaagcaac tagaaaattc ctactatcgt tttgaaagct
    32341 tgaaaaaaag ttatgaagaa aaaacgatat taataaacaa gcaaaaggaa ttagctgctc
    32401 aaaaagacga aatagaggaa aagaaacgat ggattgggta ttatgaattt acgcaacgat
    32461 tagtcgaacc aatccagcgc ctacaagaaa taaaagttga aaaaatagaa ctgtcacaaa
    32521 aaagtagtga gcaaacaaag aaactagcga tgactacgca gaattatgaa gagtggcaaa
    32581 aaaatgaagc aaaccgcgta cgccgacaaa caaaaatcaa agaagctgct caaaaactgc
    32641 aaaacaaaca gatgatggtg ccaattgtcg aatccttacg tagtaaaaaa caagacgcag
    32701 aacaattaac aatttcattg gataaagaag aattgaatgc cgagaagctg tatcaaaatc
    32761 tcgctatcta tcaggaaaga actcgaattc ttcaagaaaa tttactggat caggaaaaac
    32821 tgcaagaaga tcgtcttgct tttggacagt taaaatatct agatgaaaag cagcaagaaa
    32881 tagaaaaaca aaaagcaaaa gacactgggt cgctcgcaca gataagagaa gaactcaaaa
    32941 aagcaactga agaaataaca aaaaatagtc agctattgaa agaacagaca gaacaagcag
    33001 aacagctgaa aagccaatgg gcaaagcagc aaattgctcg tcttcagctt tttttgcgtc
    33061 caggggagcc atgtcttgtt tgtggatcaa cggaacaccc catacaacaa ttaaaacacg
    33121 aacagccaaa taaggaagat atcttaaaag tagaacaaca actggcagaa gctgagtctg
    33181 ctgtagaaag aactcagcaa atcattgcca aaagtgaagc acatgaaaat aatcttcaac
    33241 aacaattatc agatttagaa caaaaaatca tggcaacgaa tatacaattg gctgaacaag
    33301 aagaacactt gcaacaggct ttgaaaaagg catttcctga gataacagaa caaaaaacaa
    33361 aaacaactct tgtagtaatc gaacaatctt tagatagacg aaaaagcaag atggccaatg
    33421 aaagaataga gctggaagaa ataagacaac aagcagaaaa gctcgaacaa caattagctg
    33481 tctacaaaca gcagcagaca gaaaaaaata atcagctcca gcaactgcaa ggagaaatca
    33541 aaagtttaga acaacaattg caagatacgg atagtaactg tttagaagag gagatacgac
    33601 gacttgaaaa acagttagaa gaagactcga atcaactagc tgttgagcag aaagcaggag
    33661 tgcaattgtt gcaagatctt catacgcttc ggacacagct tgatcttcaa catgaacaaa
    33721 tagaaaaaat agatgaaaaa atagaaaagg aacaagaaag aatagctaaa aagttagaaa
    33781 gccagtcgta tttttcaaca attgaagaaa tctgtgcatt tgcacaacag caagcacaat
    33841 ataaagaaaa ccaacgtaca gttacttctt atcatgaaga gcaattagtg acagctgacc
    33901 ggctgcgtca attagaaaat atccagctaa ctgagacatt cccagataca gaagagatca
    33961 gccaacagtt agaagaaata agacgtatga tcgaaataaa gcaagaaaag atatacggtt
    34021 tacaagaaca aaaaattagt aatcaaaaaa tctacgatga atgtctttcg atttatcaca
    34081 caagccagac gaaattagat gagcttacgc aattacagca gctttcgcaa acattcaacg
    34141 gagaaaatcc taagaagact agtttggaac ggtatgtgct tcaggtttat cttcaagaag
    34201 tgttacaagt agcaaatgat catctccaac gtttaacgaa aaatcgttat caatttgagc
    34261 tagcggatac cattggcagt tatcgaggga aaaccggatt agaaatcaat atctatgatg
    34321 atcaggcagg tatgtctcga agtgcgcata ccctgtctgg cggtgagagt tttattgcag
    34381 cactttcttt agcattagct cttgccgatg tgatccagac acaagctggt ggtgtagcaa
    34441 tcgatgcatt gtttattgat gaaggatttg ggtcattaga tgaagaagct ttggaaatgg
    34501 caatggaagc tttagaaacg atcgaaagtg aaggaagaat gatcggcatc attagtcatg
    34561 taagagaatt gaaagaacga gtattacaac aaataagaat tgatacagaa ggtagcggac
    34621 aaagcaaaat acgctatcaa cttggataaa aagggagatt ggtaactatg aaatggagta
    34681 ttccagaaaa agtcattgca cgtggacgtc aatacttgca agaaggccga gtgttatcgg
    34741 ttgtccctga ccaagaagaa aacgtgtggc atgcagaagt cttgggcagt gaattgtata
    34801 tggttgattt ggatggtaca gcaaaagaga tagattactg ccaatgtccc tattgggagg
    34861 aacataaata ttgtaagcat acagttgctg tggaacttta tttacggtcg aaaaatctat
    34921 ctcgtatcat gaaaaaagac cagccaatgc tagaaccggt aaaagcaaca agtgagggag
    34981 aaatcattgc aaaaggtttt tctcgtctaa aaacacaaaa caatcctcaa gcggtgcaac
    35041 ctttgattat tgaataccaa gtagaaacga ttgagactaa tcagtatcac ccagagcttt
    35101 ctattttggc actttacttg cgtatcggtt cattggcatc accgaaaaaa acatatatcg
    35161 taaaaaacat ctatgatttt ttacaagctt acacagaaaa agaaacttac actgttaata
    35221 aacagtacca gtttcgtcta gataaacggg cgtttcaaaa agaagatcaa aaaatactca
    35281 cgcatcttgc aggaagtgct cagacgcatc aattattagg tacgaacggc ttacaagtca
    35341 aaggaaaact agataaaaga tatctgcttc tcccagtaga acaagcccgc ttcctgttag
    35401 agcagatgaa tcagactaca cgtttgcatc tgcagataaa tgaacaaaac gatcaaggaa
    35461 tacaattttc tgatcaaaac aaacctttat cattcaaagt ggaaaaattt ggagaagatt
    35521 atttgctgca agcattgaat gattttgatt ttttctttat gcattatcag tggggagtta
    35581 tccaagggac attttattct ctgacgaaat atcaacaaac ggtttatcaa acttggaaac
    35641 aattgattcg ccgattggag aaaccagaag tagtgttcaa gaaaaaagaa ttaccgtctt
    35701 tgtttaaaga gattatccct ctgctgtcag agatcggcga cgtagctatt gcccctgcag
    35761 tagaagagga agtagcagat atccccgtcg aatttatttt ctttttccga aaagcaaaag
    35821 gaaaaatcca agctcgagtc gatttcgtct atggtgaagt catctattcg actgacccga
    35881 agcatgaggt ctcgacagaa caggcttttc aggtgttacg tgacacagta gaagagaaac
    35941 gagtacttga tctattcaga atgtatcgct accagaaaaa tgaaactggt tatgaacgtc
    36001 cattgccaag cggggaagaa ctatatgcct ttttccgcac agaactatct gtcttccgtc
    36061 agtttggtga agtcagactt ggaagaaaat tgcgagagtt gtatttagat gcgcataaac
    36121 atcaaccgaa aatcgaagta accgagaccg gttcatggct cgatattcga ttcgatgtga
    36181 cagggataga ggagcaagaa atcgatcatg tcctgcaaag cttgctgaga aatgatgctt
    36241 tttatacatt agaaaatggt caggttcttt cttttgattc agaagaattt caacaaacaa
    36301 gccaagtgtt gcaacaatta cgggaatcga tacgtacaga agaaggaacg atccatgtac
    36361 ctaaaaatca aggattgatc attcaaaatc aattggaaaa aagcaatgct acttttagtg
    36421 aatcgttcca aacgatggta caagatctca ttcatccaga aagataccag gcacagcttc
    36481 ccaaaggatt gaatgcaacg atgagagatt atcaaaagca aggttttcga tggttgaaga
    36541 tgttaggtca ttatcagttt ggcggtattt tagccgatga aatgggactt gggaaaacac
    36601 tacagacaat tgccttttta ctttcggaaa aagaagaacg gaaaagcttc tctgctttga
    36661 tcgtagcgcc tgcgagctta atttacaact ggcaggcaga ggtgagaaaa ttcgcccctt
    36721 ctttaagtat ccaagtaatc aatgggaata aaaaagaacg tgaagaatta ctggctaaag
    36781 acacagatat ccgagtgact tcttatgcca gcttaagaca ggatctagct aattatcagt
    36841 cgcaaaaaat tgattatttg attcttgatg aggcacaaat ggtcaaaaat agcagtacga
    36901 aaactgcaca agcattgaga gaattagctg tacctcagcg gtttgcactg agtgggacac
    36961 caatcgagaa taatttagaa gaattgtggt cattgtttgc aacgattatg ccaggatttt
    37021 tcccgactaa aacaaaattc agggaacttg ccgcagaaga aattgcgcaa atgattcgac
    37081 cgtttatttt acggcgagat aaacaaactg tgttgaaaga cttgccggaa aaaacagaga
    37141 tgaatctata ttctgcactt acagaagaac aaaaaactgt ttatttagct tacttgcgtc
    37201 aaatgcgtga agaaatcaca tcaatggatt ctgaagcatt caagaaaaac cggatcggga
    37261 tacttgctgg tctgacaaga ctgcgacaaa tttgctgcga tcctcgtctg tttattgaag
    37321 attaccaagg cggttccggc aagctcgaac aagtaaaaga tctactggta gctgccaaag
    37381 agaacaaacg aagagttttg ctgttttctc aatttacagg gatgctgact atcttgcaag
    37441 aagagttagc cgagctaggg atctcgacat tttatttaag aggcagtacg aaaccacaag
    37501 atcggttaag tatggtagac gcatttaatg ctggcgaaaa agatgttttt ctgatttcct
    37561 taaaggctgg gggaactgga ttgaatctca cgggagcaga tacggtcatc ttgtatgacc
    37621 tttggtggaa tcctgccatt gaagaacaag ctgctggacg tgcacatcgg atcggacaaa
    37681 aaaatgtggt agaagtgtgg cgtatgatct ctgaaggaac gattgaggaa cgcatggatt
    37741 cactccagca agaaaaacgc gaactgttcc aaaaagtgat ccaaggtaat gaagaacagt
    37801 tgacgaaact gactgaagaa gacatccgca tgattttaag tgtaggagaa atcgaggagt
    37861 aagagactcg aaaaagcgtt cagtaatggg taagaaagcc tttctaaaga agcgattgtt
    37921 tatttgattt aaaaaatagc aaggaggctg ggattccatt ttatcccagc ttccttgcta
    37981 tcattttttt atttctattt ttaaaggtta tacaaagatt gtcttcattg tctctaacag
    38041 ctgatcaaaa gaagaaatct cgatatcgca aattggagtc tgttcaattt tgcgttctcg
    38101 atggttgaac caaagcgctt gccagttagc actttttgca cctagaacat cattgtcgta
    38161 attatcgcca acgtataagg tctcttctgg aagcatatga aattctttct cagccagctg
    38221 aaaaatctct ttttctggtt tttggaaacc tgttgcttga gaaatgagca tattgcggga
    38281 aggaatccaa ttgttcaatt gaagttgctt cagttttttt gtctgatggt cagtcggccc
    38341 atttgtgata attcctaatg ggacattctt ttccttcaag aaatcgaggg tttttttgac
    38401 ctcctcatgc aagcagatat tatcaagttc ttcctcgtaa attttttgaa ataagaggcc
    38461 atcttcttcc gtaatatgag ggtagtctaa atcttttagt gattggctga tccgatgtgc
    38521 tcgcatatat tctagtgtcc aatctcctgc cattactttt gggaagtttt catcactatg
    38581 atggcggaac cggatatata gtggatgcat atctgcatct gaaacaagcg gaacgactct
    38641 tttgaccgcg tttcgaaatg gctgctgctg atcatacatc gtatcatcaa catcaaacac
    38701 aactgattta atcaaaattt tacacgcctt tcggttttca ttgatttttt cacaaatcct
    38761 tgactattgt aacagaaaaa attgtctaaa aaagctgata aataaaggtt tttcttacta
    38821 tatttctatt tccttgacag aataaggttt ttatatttca gtttttttca ctaaaaatcg
    38881 tgaaacacgt caataagttt gatgaacaaa ttaatcatta tggta
//
LOCUS       DACDMM010000009        35180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDMM010000009 DACDMM010000000
VERSION     DACDMM010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.009"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(87..572)
                     /locus_tag="IWD16_000350"
     CDS             complement(87..572)
                     /locus_tag="IWD16_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9087436.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1052..1693
                     /locus_tag="IWD16_000351"
     CDS             1052..1693
                     /locus_tag="IWD16_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9087437.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1712..2347
                     /locus_tag="IWD16_000352"
     CDS             1712..2347
                     /locus_tag="IWD16_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9087438.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2365..3180
                     /locus_tag="IWD16_000353"
     CDS             2365..3180
                     /locus_tag="IWD16_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9087439.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3499..4935
                     /locus_tag="IWD16_000354"
     CDS             3499..4935
                     /locus_tag="IWD16_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9087440.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5124..7571
                     /locus_tag="IWD16_000355"
     CDS             5124..7571
                     /locus_tag="IWD16_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9087441.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7801..8868
                     /gene="rlmN"
                     /locus_tag="IWD16_000356"
     CDS             7801..8868
                     /gene="rlmN"
                     /locus_tag="IWD16_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9087442.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9061..10164)
                     /locus_tag="IWD16_000357"
     CDS             complement(9061..10164)
                     /locus_tag="IWD16_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9087443.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10717..11265
                     /gene="aac(6')"
                     /locus_tag="IWD16_000358"
     CDS             10717..11265
                     /gene="aac(6')"
                     /locus_tag="IWD16_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9087444.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            11811..13472
                     /locus_tag="IWD16_000359"
     CDS             11811..13472
                     /locus_tag="IWD16_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9087445.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(13542..13889)
                     /locus_tag="IWD16_000360"
     CDS             complement(13542..13889)
                     /locus_tag="IWD16_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP9087446.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            13973..14914
                     /locus_tag="IWD16_000361"
     CDS             13973..14914
                     /locus_tag="IWD16_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9087447.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            14915..15958
                     /locus_tag="IWD16_000362"
     CDS             14915..15958
                     /locus_tag="IWD16_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9087448.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            15964..17034
                     /locus_tag="IWD16_000363"
     CDS             15964..17034
                     /locus_tag="IWD16_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9087449.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            17024..17977
                     /locus_tag="IWD16_000364"
     CDS             17024..17977
                     /locus_tag="IWD16_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9087450.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            17989..18999
                     /locus_tag="IWD16_000365"
     CDS             17989..18999
                     /locus_tag="IWD16_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9087451.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(19284..20105)
                     /locus_tag="IWD16_000366"
     CDS             complement(19284..20105)
                     /locus_tag="IWD16_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9087452.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(20202..20480)
                     /locus_tag="IWD16_000367"
     CDS             complement(20202..20480)
                     /locus_tag="IWD16_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087453.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            20748..22757
                     /gene="metG"
                     /locus_tag="IWD16_000368"
     CDS             20748..22757
                     /gene="metG"
                     /locus_tag="IWD16_000368"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP9087454.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(23236..23823)
                     /locus_tag="IWD16_000369"
     CDS             complement(23236..23823)
                     /locus_tag="IWD16_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087455.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            23998..25320
                     /locus_tag="IWD16_000370"
     CDS             23998..25320
                     /locus_tag="IWD16_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9087456.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            25475..26251
                     /locus_tag="IWD16_000371"
     CDS             25475..26251
                     /locus_tag="IWD16_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9087457.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            26244..26813
                     /gene="rnmV"
                     /locus_tag="IWD16_000372"
     CDS             26244..26813
                     /gene="rnmV"
                     /locus_tag="IWD16_000372"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP9087458.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            26806..27690
                     /gene="rsmA"
                     /locus_tag="IWD16_000373"
     CDS             26806..27690
                     /gene="rsmA"
                     /locus_tag="IWD16_000373"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP9087459.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            28141..28770
                     /locus_tag="IWD16_000374"
     CDS             28141..28770
                     /locus_tag="IWD16_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087460.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(28894..30003)
                     /gene="ugpC"
                     /locus_tag="IWD16_000375"
     CDS             complement(28894..30003)
                     /gene="ugpC"
                     /locus_tag="IWD16_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP9087461.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            30212..30634
                     /gene="mgsA"
                     /locus_tag="IWD16_000376"
     CDS             30212..30634
                     /gene="mgsA"
                     /locus_tag="IWD16_000376"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP9087462.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            30957..31493
                     /locus_tag="IWD16_000377"
     CDS             30957..31493
                     /locus_tag="IWD16_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9087463.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(31533..33296)
                     /locus_tag="IWD16_000378"
     CDS             complement(31533..33296)
                     /locus_tag="IWD16_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9087464.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(33299..35014)
                     /locus_tag="IWD16_000379"
     CDS             complement(33299..35014)
                     /locus_tag="IWD16_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9087465.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT        11456 a         6269 c         7290 g        10165 t
ORIGIN      
        1 tgattggcca aaagaatttc tgatatattt tgtgctaaat aaagaaaaag acgcttgaat
       61 tgggaaataa gaaaactact ctatcattac aaattttcat aaactggcag atgttctaag
      121 taaacaagta tcttttcaaa gtcggaaaaa tccataatca acgtagtggt attgatattt
      181 ggatgaaagc ctacggtatc ttgacgatca atagcagcat cgatcacgac ttggattttt
      241 ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac ctgctggaac gcctaggagg
      301 tcaggaagct gttcttcaga aacaaaagat aagcgctttt cttttaattg ttcagctagt
      361 tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa gatagatttg ttttcctttt
      421 cttgatttca ataatagatt ttttacttgc ggaccaggta gggaaaaagg gacatttttg
      481 actgacgtaa tcgcaggatg atcgactttt tgaaaagaga tacccagttt ttttagtagt
      541 tcataagctt cttgttcggt tgcacgactc atcgcgcatc accctccttt ttttattagt
      601 gtatcataaa aaagagttga caaatgaaaa ataatcatgt aaattacacg taaagttaaa
      661 acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt tagagagtct ctggttggtg
      721 gaaagaggca gcagttaacc gtgaaacaca tcttggagtc gattttctga agcgtatagt
      781 aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct agatgaccag ataaggctgg
      841 cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt gattaacgtc ctttgatctt
      901 cggatcaaag gactttttgt tttcatctga acggcaacga ctgtgtgagg ttttttctgc
      961 aaggaaaaaa taaacagagg tggaaccacg tataatacgt cctcttggca tttgccaaga
     1021 ggattttttt attttaaagg aggaaaagaa gatggagtat ttgattgaag ttatgccgca
     1081 attattagta ggagcactca caacattgaa attattttta tttaccttga ttggctcatt
     1141 accattaggg attgttttag cattaggatt gtctttgcat tttaagccgc tacgctactt
     1201 gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg ttgttgcaac tgatttttat
     1261 tttttatggg ttgccgacga ttgggatcgt ttttgatcgt ttcgaagcag cactcattgc
     1321 ctttatctta aactatgcag cttattttgc agagatcttt cgtggaggga tacaatcgat
     1381 tcctcaaggt caatatgaag cagcgcaagt tttgcaatta agtccctttc aaacggtgac
     1441 gaaaatcgtc atcccacaag tgacaaagat tgttttacct tctgtaggga atgaagttat
     1501 caacttggtc aaagattctt ccttgatcta tatattaggt ttaggtgacg taatgcgtgc
     1561 tgggaaaatc gcgatggaac gcgacgcgac tttactgcct ttgctaggcg tggcaatcat
     1621 ctatcttaca ttgactggta ttctaacaat cataatgaaa caagttgaag gaaaattaaa
     1681 ttattaccgc taactgatag gaggaagaaa tatgattgaa ttgaagaaag tgaacaaagc
     1741 atttggtgaa aaaaaaataa tcaaagattt gaatctaatc attccagatg ggaaaacatt
     1801 agctatcgta ggtccttccg gtggcggtaa aaccacgctt ttacgtatct tggctggctt
     1861 agaaagttct gacagcggag aattttattt agacggtgta ccgttcaacc cattcgagct
     1921 taaaatgaaa gaacaagttg taggagtcgt attccaagac ttccagctat ttccacatct
     1981 gaatattttt gaaaacatta ccttagcacc aaaaatggta ttgaaacaga caaaagaaga
     2041 atacaccaaa aaagcagaag aattagcggc ttcattaggg attagtgatt tattgaatat
     2101 ctatccttat cagctttctg gcggacaaaa acagcgggta gcgattgcca gagcattggc
     2161 tatgaatcca cgggtattgg cttatgatga accaacaagt gctttagatc ctgaattacg
     2221 ccagcaagtc gagcgtttga tcctatcatt gaaagaagat ggggtaaccc aaattattgt
     2281 cacccatgat ttagtatttg cagaaaatgt cggtgatcaa ttactaaaag tttccccaat
     2341 tcattaagaa aggagaagat agagatgaaa aaaaccagtt ttctattcgc tttgatcgca
     2401 ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt cagcagataa ttgggcgact
     2461 atcaacgaag agaaaaaaat cgttataggt ttagacgata catttgttcc tatgggattt
     2521 agagataaag acgggaagct cacaggattt gatattgatt tagccaaagc agtctttgaa
     2581 caatatggga ttacggtaga ctttcagccc attgactggt caatgaagga atttgaactg
     2641 aacaatggaa cgatcgattt gatatggaac ggctattcca aaacgccggc aagagaaaag
     2701 aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag tgctgatcac acctaaagaa
     2761 tcaggaatca caaactttgg acaaatgaat aataagagat taggtgcgca aaatgggtca
     2821 tctggttatg atgtatttac gaaacaacca gaagtattaa aagagatagt caaaaaccaa
     2881 gatgctgttt tgtatgatag ttttaatgaa gcattgatcg atctgaaaag cagacgggtc
     2941 gatggtctgc tgatggataa agtatacgca gactattatt tgaaacaaag aaacgaacta
     3001 gaagacttca acataacaaa aggttcttat gagagtgagg attttgcagt tggtgcacgc
     3061 aagtcagata gtacattagt gaaaaaaatc aatgaagccc tagatcaact agagaaaaca
     3121 ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag atgttacgcc gagaaattaa
     3181 caaatagggg actgtgacaa aagacgtgtc acagtccctt tttccgaata aacagtgttc
     3241 tcctgccaac tccttgtgac tcggagctga atggcaggct cacaacctct tggtaaacgg
     3301 tgttcaaaag ccagtttttc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     3361 ttcacaaaat ttttcttact actcgaaact aagcgacttt ttcacaacct cctttacata
     3421 attttatttt gttgtggaag gggtttcgtg ttattctaaa agaggaacga ttgaaggtta
     3481 taggaaagga agagatgaat ggaacacaaa gaacttacct catttccaaa agattttctg
     3541 tggggctctg cttctgcagc gtatcaagta gaaggagctt ggcaagaaga tgggaaagga
     3601 gaatcagtct gggatcgatt tgtacggata cctggaaaga catttaaagg aacgaacggt
     3661 gatttggcag tagaccacta tcatcgttat aaagaagata ttgctttaat gaaagaacaa
     3721 ggtctgaaag cttaccgctt ctctgtagct tggacacgga tttttcctaa tggacgagga
     3781 gaagtcaatc aagctgggct cgctttttat gagagattga tcgacgaatt gattgaaaac
     3841 gatattgaac ctatgttgac tttatatcat tgggatcttc cacaagcatt gcaagatgaa
     3901 tataatggtt gggaatcacg tcagatcatt gaagatttca ctcattatgc agaaacatta
     3961 tttgaggcat tccgcgggaa agtccattac tggattagtt tgaatgaaca aaatatcttc
     4021 acttcattag gttatttgtt agcagcacat ccgccaggtg tgacagaccc gaaacgaatg
     4081 tatgaagtta accatattgc taatttagca aatgcatctg tcatcaataa atttcacgag
     4141 ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata cgccaaatta tccaattgac
     4201 agtaatcctg aaaatgttct ggctgctgaa aatgcagaag acctgatggc caattattgg
     4261 atggatgtct atatgtgggg aaaatatccg attgctgcca tgaggttctt agaagaaaaa
     4321 ggatgggcac caacgattga agctggcgat gcagaattgc tagaatctgc taaaccagat
     4381 tttcttggga tcaattacta tcaaacggcg acgaatgcat tcaatccatt aaacggagtt
     4441 ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat cagaagaaac tggagttcct
     4501 ggaatgtata aaaaagttga gaatccattt gtcgaacgaa caaattggga ctgggagatt
     4561 gacccagaag gattaaggat cggactgcgt cgtatcacaa gccgttaccg catgccggta
     4621 ctgattacgg aaaatggctt aggagagtat gacaagttga cagaagataa acaaatccat
     4681 gacgactatc gaatcaatta cttacaaagc cacgtcaaag ccatcaaaga agcaatttcg
     4741 gatggagcag aagtcctagg ctattgcacc tggtcttata cagatttgct tagctggctg
     4801 aacggttatc aaaagcgata tggatttgta tatgttgatc aagacgaaac acaagaagga
     4861 tctttggaac gatataagaa agacagttat tactggtacc aaaaactgat tgaagaaaat
     4921 ggagagaacc tttagttcac atacttaaaa caagaaggta cggctggata tggccgtgcc
     4981 ttttcttgat aaaatcattt agaaattaag aaaaactgta aaataagctg aaaaatgaaa
     5041 gaaagcgtca gttctttcaa acaaaacttt agttccctta cttttttttg tataatgaaa
     5101 cttgatgaaa tgagggataa taaatggcac aaaagaaacg gcctactaaa aaaaggaaaa
     5161 agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca aatgctgacg attggtgttg
     5221 ggtttgcctt gattttattt gctgtcttcg gctttttaaa attaggtttt ttaggaatat
     5281 tgattgcaaa cggctttcgg atcattgcag gaaatactta ccaaatcctc tgtcttttat
     5341 tagccgtatt aggattttgg attgtcatta agaatacaga gttttcaatt ggtaaaaacc
     5401 gacgctggtt tggcggcata ctcttttatt tcggtatctt attattgtta catgcccatt
     5461 tatttggcaa actccatact ggggaaccaa atatcatggg gacgacatgg gatttgttag
     5521 ccagtgatat aaaacaaagt caagtagaca ataacgttgg tggtgggatg attggagcaa
     5581 tcctttatca ttttacttac ttcttgattg cacagcctgg aagttattta gcagcagtgc
     5641 tattactggc aggaggagca ttcctgttca gtaatctgga aggctatcag ctgttaaatg
     5701 gtatccaatc aattggagaa cgagtacagg aattattgga aggtgatcct gccaagcaag
     5761 ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg cgcagaagcg aaagaagcaa
     5821 ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc agaatatgag aagaaaaaag
     5881 cggtgagcca gcaaagggaa aatactgctg atgaatggca ggaaccagaa aatacagagc
     5941 cggagcagct aagttttgtt ccaatcgaca gttttcaaga aaatatccat cctgcgaatc
     6001 tagagaaacc agtaccagat acaccaaaac agaccaatac agcagaaggt ttttcagatg
     6061 agttgccaga ggatgacggt acctcattag aatttgagat tgaagcagaa caggaaaatc
     6121 aagactatga attaccgaca gttgatttgc ttgattctat accaactgtg gatcaaagtg
     6181 atgagtataa aaaaattgaa aaaaatatcg gcgtattaga acaaactttc caaagtttcg
     6241 gtgtcgatgc caaggtcgtc aaagccagct taggaccatc cgtcacaaaa ttcgaagtcc
     6301 agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt aacagatgat atcgcgcttg
     6361 ctttagcggc gaaagatgtc cggatggaag caccgattcc aggtaaatca ctgattggta
     6421 ttgaagtacc aaatggaaag atcagtatgg tttcctttag agaaatcatc gaagcacaac
     6481 caaaccaccc agataaatta ctagaagtac ctttaggacg tgatgtttct ggacgggtac
     6541 aaacggctga tctttcgaag atgcctcact tactcgtggc tggttcgact ggtagtggga
     6601 agtcagtagc aattaatggg attatcacaa gtatattaat gcgtgctaag ccccatgaag
     6661 taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa tatgtacaac ggcatacctc
     6721 atctgttgac gccggtcgtg acgaacccta gaaaagcagc gcaagcctta caaaaagtag
     6781 tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac cggagtaaga aatatttctg
     6841 gttacaatga atttgttcaa cagaaaaact tggaaaacgg aacgaaacat ccaaccttac
     6901 cattcatcgt agtgatcgta gatgaattag ctgatttgat gatggtagcc agcaatgaag
     6961 ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc agcggggatt catatgatcc
     7021 tagctactca acgtccaagt gtggatgtta tcacaggaat catcaaagca aatgtacctt
     7081 ccagaatggc atttgcggta tcaagcggta cagattctcg gacgatcatt gattctaatg
     7141 gggcagaaaa gctcttaggg cgaggagata tgctattctt gccaatgggt gagaataaac
     7201 cgatccgtgt gcaaggagca ttcatttctg accacgaagt agaacgggtc gttcaatttg
     7261 ttacagatca gcaagaagct cattatgaag agaaaatgat gccgacagat gaagttgaaa
     7321 cgacaggtgc tccagaacag ccacaagatg agctgtttga agaagcaaaa gcacttgtcg
     7381 ttgaaatgca aacggcaagt atttcccttc tccaaagaag atttcggatc ggttacaacc
     7441 gagcagcacg tttagtagat gaactggaag ctcatggagt tgttggcccg tcagaaggaa
     7501 gcaagcctcg gaaagtattc attgagcagg aagaaacatc agaaacgttg ccggacgata
     7561 atgacatgta aaacttgtgt gatgaagggt tgaaaaacga ataaccaata aaaagacaag
     7621 gcatgatgat accaaaagaa aaaatggttt ttttccatct tttctaatac ggtatgatgt
     7681 cttgtctttt tagtttttgt gagattaaag ttctattcga tttatttgtg aagtattgca
     7741 ttaaggaaat gaatcatgta tagtatgtag cgaaactttg agaaaaagag ggaaaatact
     7801 ttggaaaaac agtccattta tggcttgacg aatgaagaat taatcaattg gtttatagaa
     7861 aacggcgaga aaaaattccg agctgcccaa gtttgggaat ggttgtatca aaaacgtgta
     7921 agtaatttca cagaaatgac gaatctatcg aaacaattga tcgaaaaatt atcggagcat
     7981 tttatcatca atcctctaaa acagatggtc gtccaagaag cttcggatgg tacagtaaaa
     8041 tatttgtttg aactccctga taaaaacatg attgagactg tactgatgag acaagaatat
     8101 ggcttgtcag tttgtgtgac gacacaagta ggatgtaata tcggctgtac gttttgtgca
     8161 agcgggctgc tcaaaaagaa ccgtgatctg actgctggag aaattgttgc acagatcatg
     8221 atggtccaac attattttga tgagcgcaat cttggcgaac gcgtttcaca tgtcgttgta
     8281 atggggatcg gtgagccatt tgataattac gacaatgtaa tggacttttt gcatatcatc
     8341 aatgatgcca aaggattggc aattggtgct cggcacatta cagtttccac tagtggatta
     8401 gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag tcaatctggc tatttccttg
     8461 cacgcgccga ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag tttcccaatt
     8521 gagaaattga tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg aatcacgttt
     8581 gaatatatca tgttgaatca agtaaatgat agaccagagc atgcgcaaca attagcagat
     8641 ctattaaaag ataagaaaaa attagcatat gtcaacttga ttccatataa tccagtcagt
     8701 gaacatgatc aatattctcg cagtccaaaa gctgatgtat tgagatttta tgatgtattg
     8761 aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg gtacagatat tgatgcagct
     8821 tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc gtatcctact
     8881 cgttttttca attaccataa aaggagcagt tttcttaatt aaggaactgt tcaacttaaa
     8941 taagagaaaa gattctatag cgaaagctat aaagcttttt ttgacaagaa aaaccctcat
     9001 caagcaaaga aatgtcctca atttgatgac agcctgcttg atgggggttt tagcaattat
     9061 ttaactgatt ttcttatatc cttgctggct ttcttgtgta gcatgataag tttctacgtc
     9121 aagaatggcc tgtttaaatt tttctcgggt gatttcataa ggcacatgtt taatatcatt
     9181 tgttgccatg gctttatcta agattgcttc taaatcggtc gtgacattga gattcatgtc
     9241 ggttaacgat actggaagtt ggttcttttt cataaacaca tagattttat caaacaaatc
     9301 gaactgctga tccatcatca ataaggtcag tacaccataa ctgacagatg ctccgtgaag
     9361 gtatccctcc attttttcta gaacagtcgt accgtaatag aatgagtgtg ccatagcaga
     9421 attatagtta ttgttaacta aaacagaaac caatccggta gtaacaatga tatccgaaac
     9481 gacttgttca attgcatatg agggacgatc gtcggcacta tctttcaatg cttgcgtacc
     9541 ataagtcaag agacgatcaa tgcacatttt tccgatttgg attcctaatc tatccgtgta
     9601 gcctagatca tcgccacgag cagaaaaagt agattcgact tctttggata aagcatcgcc
     9661 aatacctgcc cataagtatt tatcaggtgc ttcggcaata attgctgtat caatgaaggt
     9721 gtgtacaggt ggttttgaag gaaagaagag tccgtccatt acgccttctt cgttatagat
     9781 gacacatacc gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg
     9841 tttagctagt ttttcaccaa tgacttttac agtatcgcaa gcacggccac caccgactgc
     9901 aaacaggacg tctgcttttt ggacactttc atgctggatc aactgttcaa tatttccata
     9961 agttgaatta cccccgtacc agagactggc gacaacctct atttcctgtg tcaaagctac
    10021 ttttattttg ttttctgcct ttgaaagtgc agtcttccct ccaatcagca ctacttttgt
    10081 cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa tggtataatt
    10141 tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa acgggttaaa
    10201 attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga acctaagaca
    10261 ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa
    10321 cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcctc ttagtatttc
    10381 taccgattcc ctttgctttt ttgtaattat ctaaaaattc taaaaattaa gattattata
    10441 tgaaaacagg ttttatttgt caattcataa ttggtttttt ttaaagcaaa agataatcta
    10501 tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga tctttcctat
    10561 tttgaataaa gtgtttctgt ataatttttt tattttataa gtaagataaa taatgaaaga
    10621 atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt ttttcaaaag
    10681 gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga atttgaccgt
    10741 aataatccag tattgaaaga tcagctttct gatttactga gactgacttg gccggaagaa
    10801 tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg aatcgcggta
    10861 gcagcggtag accaagatga gttagtagga tttattggtg caatccctca atacggtatc
    10921 acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa ccaaataggt
    10981 actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat cacgatttat
    11041 ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct gtatgagcat
    11101 acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga attctatgaa
    11161 aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga caaaccggat
    11221 atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa agaggttgtg
    11281 acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt cttgaatttt
    11341 tgtgaaattc caacttattt ccgaagaagc tacttttgga acaccgtgta ccagaggttg
    11401 tgaaaaagtc gcttagtttc gagtagtaag aaaaattttg tgaaaatggc tttctacatt
    11461 tttgcataaa tttggcttaa taccgagaaa ctggcttttg aacaccgttt attcagataa
    11521 gggactgtga caagactttt gtcacagttt ctttttgttt attctttttt atctttgtat
    11581 ggcaataata gacaatataa cgaaaattat ttaatggctt ttggaagacc atggaaaacc
    11641 ttgtacaaag ctattttttc tgaaaaattc cttgaaacct cacgaaaaaa tcattatttt
    11701 atgaaaaaga caaaaaaaag tgttgatttt ttaataatta ttcattaaac tcttaaacaa
    11761 cttcagaaaa tattaattat tctgaaaatt ccaataaagg ggtacgtaaa atgaaaaaga
    11821 aatttacatt tggtttggtt gcggtatgcg gccttgtact tgctggatgt tatggcggca
    11881 gttcagccac aagcagtagt tcagcaaaaa gcggttcagc agatggagga ggcgtattta
    11941 acttagtcgt gcctcaagaa atgcctacag ctgacttatc agttgcaaca gatacaatca
    12001 gttttacagc attgaataac gtttatgaag gaatttaccg tcttgatgaa gacagcaaac
    12061 cacagccggc aggagctagc gagttggcag aagtcagtga agacggatta acttacaaaa
    12121 tcaaattacg agaagacgca aaatggtcaa atggtgatcc tgttacagca gcggattacg
    12181 tttatggctg gcaacgaaca gtagacccag ctacagcttc tgaatatgct tacctgtatg
    12241 cgccagttga aaatgcagaa gacattacag ctggaaagaa agataaatca gaactaggga
    12301 tcaaagcagt tggtgactat gaattagaaa tcaaattgac gaaacaaaca ccatatttcc
    12361 aatatctgct tgctttccca tcatttttcc cacaaaaaca atccgtagtg gaagaaaatg
    12421 gggatgccta tgcatctgca agtgataaag ctgtttataa tggaccattc acattagaag
    12481 attttgatgg accaggaaca gatacagaat ggacttataa gaaaaatgat cattactggg
    12541 acaaagatac agtaaaactt tctgaaatca aagtcagtgt agtaaaagaa tcttctactg
    12601 cattgaactt attcaaagat ggccaagcag atgacgtgat cttgtctggt gagttagcac
    12661 agcaaaacgc aaacgatcca gcttatactt ctgttaaaga agcgcggaca agctatatcg
    12721 aattcaatca acgtgaaaag aattcaccat tcaacaatgt gaacctaaga aaagctattt
    12781 cttattctat cgatcgtgaa gctttagtaa aacaagtact aggtgacgga tcagttgtct
    12841 ctacaggatt gatcccatca gatatggtaa aaagtccaac aacgaacgaa gactttgcaa
    12901 aagaagctgg aaaattagtt tcttatgaca aagataaagc aaaagaatat tgggaaaaag
    12961 cgaaaaaaga attgggcgtt gattctttag aatttgattt gatggcttct gatgatgatt
    13021 catcgaaaaa agtaatcgaa tatgttcaaa actcaatcca agaaaacttg gatggcgtaa
    13081 cagtcaaacc aacacctgtt ccattctctg tacgtcttga ccgctcaact tcgggtgatt
    13141 ttgacgcagt actaggcgga tgggctgctg actatgcaga cccaagcagc tttactgatt
    13201 tgtttgtcac aggtaattca tacaaccgtg gtcgctggag caatgcagaa tatgacgaag
    13261 cagttaaagc agcttcatca acagacgctg gcgatgaaca agcccgttgg gatgatttcc
    13321 aaaaagcaat caagatcatc tctgatgata tgggtgtcgt tccggtgtac caaaaagcag
    13381 aaggtcacct agtgaatgaa aaaatcaaag gtatcgttca ccatgcggca ggcgcttcat
    13441 gggattacaa atggacatac gttgaagaat aatcgttaat accgtgagca tgagaaaaaa
    13501 cgaggctgcg gcaaatgtcg caacctcgtt ctcatattca cttatcctaa caaagaaaaa
    13561 agcatgaaaa tagcagaaag agcaattagt attccggcaa tgatcagcca aaagctgtac
    13621 ataccgtagc tggcagacga gagcgaggaa aattctggtt ttttctttcg gttccttgtt
    13681 cgtgcaagat ggaccgatcg ctgaaaatga tcgaagaaac ccgaagaaaa gacccataaa
    13741 aaaccgccga taatcagaaa cggcaatgac cataagaata gatcattaga taattgaatg
    13801 agtgtaaagg aatggttgat gagatttttt attaaaataa ccagaatcag tagaaaagct
    13861 actgcgtagg ggatagattt ttttttcatt tgattgctcc tttatttttg gttcattatc
    13921 taataatgta gcgaaacatc tgtcagaagt caaaagctag ggggaaacat tagtgaatag
    13981 ttatatcaaa tatgtattaa aacgtgtctt tttcatgatc atcacgcttt ggttaattgc
    14041 aacaatcacc tttttcttga tgcaattgct gccgggcacg ccatatacga atcaggaacg
    14101 tttgagtcct gaaacaatcg agatgctaaa taaacaagtc ggtttggata aaccagttat
    14161 cgtacaatat gggatttatc tttctaattt gcttcaagga gattttggaa tttccttcca
    14221 atttaaaaac cagccagtcg cacatttgtt agctggaaga atcggaccat ccttgcagtt
    14281 agggttacag gcaattatct ttggaacagt cctagggacg attttaggaa cgatctctgc
    14341 catgaagcaa aatacatggg cagatacatc ttctacactc gtagcgatat taggtcgttc
    14401 cattccaaac tttgtatttg ctgttttatt acaatatatc tttgcaatca aattacaagt
    14461 tttgccaatc gcaaaatggg atggctttgt gtacacaatc ttaccaacaa ttgcgttggc
    14521 catgtcgccg cttgctgact ctgctcggtt tatccgtacg gaaatggtgg aagtactgca
    14581 tagtgattat gtggaattag caaaagcaaa aggattgagc cgttgggaaa tcgcctttaa
    14641 acatggtctt cgaaatagtt tgattccatt gatgacttta ttaggaccgt tagctgtagc
    14701 attgatgacc ggatcactgg tggtagaaaa tatctttgct attcctggta tcggggaaca
    14761 attcgttaag tcaatcacaa caaatgatta tcctacgatc atggctgtaa cgatccttta
    14821 ctcatttatg ctgatttttg ttattttgat cgtggatcta ttgtacggtc tagttgatcc
    14881 aagaattcgt gtttcagaag ggagccgagg ataaatggaa atgataccaa agcgttatga
    14941 aacagttgca gatatccctg cggatgaatt tcttccatta caaaaaaata ccgaagaaga
    15001 acgtgaacag attactgcac cttctttgaa ttttatccaa gattcatggc gccgtttaaa
    15061 gaaaaacaaa gctgccgtta tttcaatggt tttactattg gtcatcattt tgatctctgt
    15121 gatcacaatc tttgtttctc ctcatgatcc aactgcccaa aacgtggctt atatcaattt
    15181 gcctcctcgt attccgggaa tcaatatcga tggattgaat ggaaaagcaa tggttggtgg
    15241 tgaacttgtt gataaatatg ctcaagcaaa cgtcccttca aacgtaaatt tctttttagg
    15301 aacagacggt ttgggacgtg atgtattaag ccgcttattt atgggaatga gaatctcgtt
    15361 gctgatcgca tttattgctg cattactaga tgtaacgatt ggtgtagctt acgggttggt
    15421 ttctggtttg ttaggcggac gtgtcgataa cgtgatgcaa cgttttttgg aaatcttatc
    15481 aggaattcca aacctcgttg tcatgatttt gatgttggtc gtttttgaac caggaatttt
    15541 ttcaatcgtg gctgctatgg ccatcacaaa ctggatacca atggcacgta tcgttcgtgc
    15601 acaaacaatg aaattaaaag atcaagaata tgttttagct ggattgacat taggtgaatc
    15661 aagaatgaaa attgcattca aacatatttt gccgaatatc tctagtgtca ttattattca
    15721 aatgatgttt agtattccat ctgctatttt cttcgaagcc tttttaagct ttatcggttt
    15781 aggtcttacg cctccgtcag cttcattagg aacgatgttg agcgaagggt acaagacatt
    15841 cctgtatctg ccttatctat tatggattcc ggcagtaact ttatcggtta tcatgattgg
    15901 ctttaactta cttgctgatg gattgcgtga tgcctttgat cctaaaatga aagagtgaag
    15961 acgatgacga aaattttaga agtgaaagac ttagaaatct cttttgatac gtatgctgga
    16021 aaggtacgag cgattcgcgg tgtcaatttt catttgaata aaggagaaac attggcgatc
    16081 gttggggaat ctggaagtgg aaaatcagtg acgaccagaa gcatcatgcg cttgctgtca
    16141 agcaacgcca atatagatgc tggtcaaatc ctctttaaag gtaacgatat cgttggaaaa
    16201 tcagaaagag agatgcaaaa aatccgtggt aaagaaatag cgatgatttt ccaagatcca
    16261 atgacctctt tagatccgac gatgcccatt gggaagcaag tcgctgagtc ccttagaaag
    16321 cataataaag tttcaaaaaa agagggacaa aaagctgcat tagaattgct taaattagtt
    16381 ggaatacccg aagctgagaa acgtatcaac agttatccgc atcagttctc aggaggtcag
    16441 cgtcaacgga tcgtgatcgc gattgcactg gtatgttatc cggaaatcct gattgctgat
    16501 gaacctacaa cagcgcttga tgtgactatt caagcacaga ttttggagct gctgaaagaa
    16561 attcagacaa aaatcgatac atcaattatc tttatcactc atgaccttgg tgtagtagcg
    16621 aatgtcgctg atcgtgtcgc tgtcatgtat ggaggaagaa tcgttgaagt tggaacatcg
    16681 gaagaaattt tctacaaccc gcaacatccc tatacttggg gactgcttgg ttcaatgccg
    16741 acattagata gtgcgaacga tcgattgtat gctattcctg gttctccacc ggatttattg
    16801 aacccgccaa aaggagacgc attctatcca agaaatgaat tcgcgatgaa aatcgatgca
    16861 gaacaagaac caccattttt tgaactatca aaaacacaca aggcagcaac ttggctactt
    16921 gctccgcagg cacccaaagt gacaccgcca gaagaaatcc aacgtcgctg ggctatcttt
    16981 aaagagaaac aaaaacatac ttacggtcaa ggaggaattg ctgatgccaa ataaactact
    17041 tgaagttaaa ggattgaaac agtatttcaa tgtaggaaaa aagaatgaag tacatgctgt
    17101 aaatgacatc agctttcaca tttatgaagg agaaacattc ggtttagttg gagaatctgg
    17161 aagcggaaaa tccactactg gccgtacgat cattcgcttg aatgaaccta ctgatggtga
    17221 gatattattc gacggtcaag atataacgaa gatcaaagat aaaaaaggtt tgacgaaatt
    17281 ccggcatgat gttcagatga tctttcaaga tccatacgct tcgttgaatc ctcgtatgaa
    17341 agtcagagat atcattgccg aaggaattga tgttaatgga ctagctaaat catctgaaga
    17401 gcgtgcaaaa aaagtcgatg atctattgag aactgttgga ttgaatccaa gccacggtac
    17461 tcgttatcct catgaatttt caggtgggca acgccagcgt attggtattg cacgagcatt
    17521 ggcagtaaaa ccgcgtttta tcatttgtga tgaaccaatt tctgcactag atgtgtcgat
    17581 ccaagcccaa gtggtcaatt tgttgcaaga tctccaaaaa gaacatcaat tgacctatct
    17641 atttattgca catgatttgt cgatggttaa acatattagt gaccgaatcg gtgtaatgca
    17701 taacggcttg ttattagaga tgggaacaag tgatgaaatc tataatcacg gtgttcatcc
    17761 ttatacagaa agtttgttat ctgctattcc tctaccagat ccagaccatg agagaaaacg
    17821 cagaagaatc aaatatcaac ctgaaccaga tgacggacaa gtacgtcaac ttcgtgaaat
    17881 cgcaccagaa cattttgtct atgcaactga gcaggaagtc ccatattacg caaaaaaact
    17941 aaaacgacaa aaggaatctc ttctggttgc taactaggag ggacatagat gctaaaagct
    18001 tttaaattcc gtatgtatcc gacagaagaa caaaaacagc aattgattcg tacttttgga
    18061 tgtgctcgat ttacctataa ccatttgctg aaaaagcggc agaaaagttg gcaacaaaca
    18121 ggagtagcca atttttcgct cacacccgct acattaaaaa aagaataccc attcttaaaa
    18181 gaagtagaca gtcttgcttt agcaaatgca cagcttaatt tggatcgggc ttttcgcaat
    18241 tatttcaagg gacgtgccag cttcccaaag cttaaaacga aaaaaagtat gtggcaatct
    18301 tatacgacca acaaccaaac ccgtacgatc tatctggaaa atggctactt aaagttacca
    18361 aaacaaaaag aactaatcaa gataaatcag catcggccag ttgaaggatc gattcgctcc
    18421 gctacgattt ctgctcgata taatgaagaa ttttatgtcg cactactttg tgatgtttcg
    18481 ccagtaaaaa aagaatcttt agctaaatgg atcgggatcg cctatcatcc gaagacattg
    18541 atccaaacat ctcgacctct agaagtaact ttaccaaaat ttcaccagac agaagaaaag
    18601 ctgcaacatg cacagcggaa attgaatgtg aaagtccgct ccgctcacca tagaaagatc
    18661 aggttagatc aggcaagcaa ttatcagaaa caaaagagaa aagtaatgga tctttatctg
    18721 aaacaaaaga accagcgaga agattatctt gaacaattat ctgggaaatt ggtcaaacaa
    18781 tatgactact tatttgtaga atcttttcca aaagaagagg cacatgctga tttttctatt
    18841 cacgactggc ataaattaat aacaaaattg cgttataagt cccaatggta taataaaaaa
    18901 tttcttttga tcaataccga cggagcagaa gaaagcaatt ccgtaagaaa gagccaagta
    18961 gtagaaaaaa tgggcagaca ttcggttatc aagggataaa aaatgaatga aactgatagg
    19021 agcgttaggg ttagccttgt taataaaaag tcttaatgac ttttgtttct aggaattttt
    19081 cttttgaaga aaagctcact aattatgaca aaccaataaa acaatagcag aaaaagttcg
    19141 tacgagcttt ttctgctatt gttaattttt tattcagaaa gtttacgaat acatcaaaat
    19201 tctgaaacaa aaaacgtaac tcacaaagag ttgtttttgc gttttttctt gaatgattag
    19261 gtctgaatct tttgctcaca accttattca tggtgccccg gcttgagctg atagattttt
    19321 gaaggacggc cgattcctac aggacgttca ccgatctcat cgaaatattg gagcatagct
    19381 ttcttaaaat tggaatgatc gatcgtttta taatcgatcc ctaagaattt agcaaatact
    19441 tttcgagctt ccgtaatcgt gaaatcttta cctaaaactt gtagaacttg cggctcgtgt
    19501 tccattttat tgactacacg gttgaatgct ttcaagatga tttgactatg gtcaaaagcc
    19561 aaagtatctt tgccagaaga ctctccagtg actaaatcaa gcgagatagc cacttctccg
    19621 ctagataaat ggatcttgtt actatgacgt tctagtgtga accagcgtac ttccttggca
    19681 tcatctccgg cgattagcgg ttcttctcca ataaaggcta agtagctgac tgtgacgacc
    19741 cagcctcgcg gatctcgatt aggcgtactg aatgtatgca gttgctcaat attttctttt
    19801 gaaataacca caccagtttc ttcctttgtt tctcttaaga cgctttctcc agttgactca
    19861 tccttttgga caaagccacc aggcaatgcc catgaatttc gataaggatg cccttttctt
    19921 tggatcaaca aaactttcag ctgatcttct tctttgttat agcacatcag tacgatatcg
    19981 acagtcaaag agggcttgtc atattctgga cgttcctgtt tttgatacca gttaagaaat
    20041 tcttcttcac ttgctttatg ttcataatat aatttttctt cttctttaga agcaaaacgg
    20101 ttcatctgac cacctccatt gacagtaaaa gtacttttta ccttattgta gcagactctg
    20161 aaaaaaaacc aaggatttac ttgaaaaatc taggttatca tttaaatatg aaaaataaat
    20221 ggaagtatga gattgaataa aaagaaaagc agataaaacg gcaatacttt ataccgctct
    20281 tcacgaatgg caaagaagcc acaaataacg atatatacgt caatgataaa acgcaaaatc
    20341 caagctgctg tcataaaact gttcaaatgc ccatggaggt taaaataacc atacatacga
    20401 atataaaaat agctgaatat taaatgtgct aatgaaagaa aagcaatact aagtgtaaaa
    20461 aaaagatgtt tcttctgcat gattgttcca gtcccttact aagatagata gtctttatta
    20521 taccacgtgt gaggggagaa ataaaaagcc gttttttagt agatatgaaa caaaacaaaa
    20581 acccgcattt attcaaccta ttcatccttt taaaggatag attgaacgta gatagaagtc
    20641 gtttatccag tctttttcat tttgaaaaaa agctaacagc atgctttttt tatggtatac
    20701 tagcaaggaa acgaattcgc atcatgcgat aaaggaggaa gagccccgtg gcagaaaagg
    20761 aaacttttta tatcactacg ccaatctatt atccaagcgg caaacttcat atcggcaact
    20821 cgtatactac gattgcatgt gacgcaattg ctcgttataa acggttgatg ggctttgatg
    20881 tgttttatct cactggagtg gatgaacatg gtcaaaaaat cgaaacaaaa gcagaagaac
    20941 taggtgtaaa accgcaagaa tatgtagata aaatggcggc tgatgtcaag aaattatgga
    21001 aaacgcttga tatcagttat gataaattca ttcgcacgac agatgattac cataaagcag
    21061 ctgttcaaaa aatatttgat cgtttattag aacagggaga tatttactta ggtgaatatg
    21121 aaggctggta ctctgtttct gacgaagaat tctttacaga aacacaactt gcagaagtgt
    21181 acaaagatga cgaaggtaac gtgatcggtg gtaaagcgcc aagtggacac gaagtagaat
    21241 tggtaaaaga agaatcatac ttcttccgca tgagcaaata cgcagaccgt ctattggaat
    21301 actatgaaga acatcccgaa ttcatccagc cagaatctcg taaaaacgaa atgatcaaca
    21361 actttatcaa accaggatta gaggatcttg cagtttcacg aacaactttt acttggggag
    21421 taccagtcaa gagtgatccg aaacatgtgg tgtatgtatg gatcgatgca ttatccaatt
    21481 atattactgc actaggatat ggctccgaag atgaaagtct attcgaaaaa tactggcctg
    21541 cagatgttca gatggtcggt aaagaaatcg ttcgctttca tacgatctat tggccaatca
    21601 tgctgatggc ccttgatttg cctctaccta aaaaagtatt tgggcatggc tggctattga
    21661 tgaaagacgg aaaaatgtct aaatcaaaag gaaatgtcgt ttatcctgaa atgcttgtgg
    21721 aacgttatgg tttagatgca ctccgttatt atttgcttcg tgctgttccg tttgggtcag
    21781 atggtgtctt tacgccagaa gattttgttt cccgtgtcaa ttatgatctg gcaaatgatc
    21841 tagggaactt attgaatcga acgattgcta tgatcaacaa atattgtgat ggactcgttc
    21901 ctgactatgc ctcacttgtc actccatttg atagtgaact gtctacgacg gctgctaacg
    21961 ttgtcggacg ttaccatgat gcaatggaaa aaatggaatt caatacagca ttggctgaaa
    22021 tctgggtgct gatttctcgt gcaaacaaat acattgatga aacagaacca tgggtattag
    22081 caaaagacga agaaaagaaa gctgaattga atagtgtcat gatccattta gctgaatctt
    22141 tgcgtatcgc agcgatatta cttcagccaa tcatgacaga aacgccagag aagatcttca
    22201 atcaactagg attggactct gaaacaatga atcttgaagg attacacttt ggcgaatttc
    22261 cttcaggaac aaaagtagtt gcaaaaggta caccaatctt cccacgttta gatatggaag
    22321 aagaagttgc ttttattcaa gaaaaaatgt ctgaaggtac tcaaacaaac gaagatacag
    22381 tcaaatggga tccagaagaa acagaacttg tttcaacgaa agaaaaacaa atcaaatttg
    22441 atgtatttga aaaagtagaa ctaaaagtag cagaagtcat caactgtcaa aaagtagaag
    22501 gggcagataa gttgctgcaa ttccgactag atgctggaga cagccaagac cgtcaaatct
    22561 tgtcaggaat cgctgaattt tatcctgatc caagcgaatt gatcgggaaa aaagtcgtca
    22621 ttgtagcaaa tctgaaacca cgaaagatgc gtggacaaat cagtcaagga atgatccttt
    22681 ctgcagaagc accagatggc tctttacaag taatcgaagc accaaaatcc atgccaaatg
    22741 gctcagaaat cgcatagaaa ttcaaagggg gtatgacact agttgtgtca tacccccttt
    22801 ttccgaaatt tgagaagcaa ttttcgtaaa ttccttctta ttacttgaag ccaatcatat
    22861 aatacagaaa cagagaacta tccaaaataa atcctagaaa aagaagtacg agcttcgctg
    22921 atttttgtta atttagaata atactaatag gaatcgtaaa aacagaaaaa aagcataatg
    22981 ataaagggtc gggcagattt taattgattc atctggtact cttttttatt gtccttacat
    23041 tcataaagtt tgaatgcaat gactttatta catctatatt tatatctaac agtagcgttg
    23101 atgtgttaat tataagttct atcactttat gttcaattag tttgtgctct tatatgtgtg
    23161 attgaaaata tagataaaaa aacaatcatc ggctttgact tcgatgattg ttttctttat
    23221 tttgtaaaaa tcctatcact tgattttatg gtaacctttt atttttgtct tcaaacggct
    23281 tggtaagaga gcagtaagca gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg
    23341 tccgatacca taatgttgat agagctttgt caacatcacg tgtaacaaga tcaaccctaa
    23401 aagcaaggag ccagctataa accaatgatt atcccaatgc atgacttcac taagtgccat
    23461 cactccaaaa ggatagaaat aaaaatagat cccgactaag gtcatccgcc agttctttgt
    23521 aaatgttccg atattcaata aaaagaaaat cgctgaaagc aaaataccga taggcggaaa
    23581 aagcccaacc aaaaaggaat acaacatccc ccacaagatc atcacaggac ctttgatcaa
    23641 tgcaacgatt gcgatgaaca aagtcagcaa tattagtttt tgtgataacg ttgcaacagt
    23701 cattaatcca aataaaatta atgtgaacca atgccaatgg gactgctgtt gttctttacg
    23761 ggaaaaatcg acccggttgg ataaataatc tgctaatttc ggataagaac gcttaaaatc
    23821 cataaactgt tcctccttcg tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa
    23881 tacatccgtc ttaagaggga gtttttcact gaaagtggag aaaaaagaat ggtaaattaa
    23941 aatattctca gttatactga aaattatacc aaatttcaaa gaagaaagag gatgggaatg
    24001 aaaggcaaac taaaacgaga aatcaatctg tttggcgcat tagctaccgt tatgggaacc
    24061 gtgatcggtg caggcgtctt cttcaaaact gctgctgtta ctgctagtac tcagtctaca
    24121 agtttgacat tgctagcttg gcttttgggt ggtttcttaa cgatctgtgc tggattgaca
    24181 gtggctgaat tagctacagc aatcccggaa actggtggag ctgtaaaata tattgaagct
    24241 gcgtatggaa aattgcccag ctttttatta ggatgggcgc aaagcttgat ttactttcct
    24301 gctaatattg ctgcattatc gattatcttt gcgacacaat tgacgaactt attacaatta
    24361 tctaccgatt atcttttgtt gattgcagtg atcaccgctg tttctgtgac agggttgaat
    24421 ttgttaggga caaaagtcgg tgcttctgta caatcagtga cgttgattgt taaattaata
    24481 ccaattgctg tgattgtcat atggggatta ttgaccccag gacaaggaac tgtccagtta
    24541 tttccaatcg aagctggaaa agatgttact tttgtcgaag gattgagcag tgcgttgtta
    24601 gcaacgttgt ttgcttatga cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa
    24661 cggccggaaa aagatttgcc taaagcaatt attcttggtt tgagttttgt aacagtcgtt
    24721 tatctattga tcaattttgt ttttttgaaa actttgccga ttgatcatct tgccggtaat
    24781 ttgaatgcag cttctgaagc atcagacgtc atttttggag gaatcggtgg aaagctggtc
    24841 actattggga tcttgatctc ggtatacggt gcattgaacg gttatacact aactgggatc
    24901 cgcgtgcctt atgcgatggc tttagaagac gagctacctt tcagtaaaca gctgacaaag
    24961 ctttcaaaga agtttacggt tccttatgtt cctgcggttt tccagttagc agtagcttgt
    25021 attatgatga gccttggttc tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg
    25081 ctattcagtt tactgatttt tatcggtgtc tttattttaa gaaaaaaagc gccggaattg
    25141 ccacgtccat ataaagtacc actttatcca atcgttccga ttattgcgat tctcggtgct
    25201 attttcattt taggtatgac gatgatgacg caaacgaaat tagcattgat cggtataggt
    25261 gttaccttga taggtatccc agtctattat caaaaaaaga aaaaacgctc agaagagtaa
    25321 aatatttcaa caacagacag aaaaagtacg aactaggtcc tctagatctt cgtgcttttt
    25381 ctatttcaat aaaacaagtt cttctatttt gatttttgct acttctgcaa gtagtgctta
    25441 tgtgctaaag taagaatgaa aaggagagat aaacatgata tttgattctc atacccattt
    25501 aaatgctgaa cagttcaatg aagatattcc agaaacgatt cagcgagcac aagaaatggg
    25561 ggtaacgaaa atggcggtcg tgggcttcga tacgccaacg attgaaaagt ctttgcaatt
    25621 aagccatgac tatccaaata tatacagtat tatcggctgg catccgacag aggctggcag
    25681 ttatacgaaa gaaatcgaaa ataaattaca agaacaattg actttaccaa aggttgtagc
    25741 attaggggaa attggcttgg attatcattg gatggaagac ccgaaagaag tccaagataa
    25801 agtatttcgc agacagattg ctatcgcaaa agaaatgaac ctaccaatca gtattcatac
    25861 tagagaagca cttgaagaca cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg
    25921 tggaatcatg catagtttca gtggtgatta tgaatgggca aaacgatttc ttgacttagg
    25981 gatgcatatt tcatttagcg gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc
    26041 tgcagcaaat gtaccaatcg aacgattact tgtggaaaca gatgcccctt atctggcacc
    26101 agttcctttt agaggaaagc gaaatgaacc aggctatact cgttatacag tagaaaagat
    26161 tgctgaactt cggaatcttc cgacagaaga agtggctgcc caaacatgga caaatgcgca
    26221 ccgattattt ggtctaagcg aacatggata agatgaagat ccaagagatt atagtcgttg
    26281 aaggaaaaga cgatacaaga aggctgcggg aagtagttga tgcagatacg atagaaacaa
    26341 tagggtctgc aatcaatgaa gagatcctga tgcaaattga acatgcccaa gagactcgtg
    26401 gagtgatcgt ttttaccgat cctgattttt caggagaaaa aataagaaaa atcattatgg
    26461 aagctgttcc tcaggcaaaa catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa
    26521 agggaagcct tggtgtcgag catgcaagtg atgctgctat tttagaagca ttgaaaaagg
    26581 tcgtgacgcc agacatttcc caaacggctg tttctgaaat cactagacaa gaccttttag
    26641 actatggttt aatagctggg gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt
    26701 tgcgcatcgg ttacacgaac ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta
    26761 cgccgaaaga actcgcccaa gcaatgaaaa tagtggagga acaacatgag tgaatatcgt
    26821 gaaattgcga caccctctag aacaaaagag attttattga aacatggttt ttcatttaaa
    26881 aaaagtttag gacaaaattt tctgactgaa ccaaatattt taaggaaaat cgtagaaact
    26941 gcgcaaatag atgaacatac gaacgtgatc gaagttggac caggaatcgg tgcattgacg
    27001 gaacaattag ccaagcatgc aaaacaagtc gtagcatttg agatcgatga tcgtttgatc
    27061 cctgtattgg ctgatacgat gcaaccctat gacaatgtga ctattgtaca tcaagatatt
    27121 ttaaagaccg atttgtcaac agctgttaga gaaacatttc atgaggagct tccgttaaaa
    27181 gtcgtagcga atttacctta ctatattacg acaccgatca tgatgcattt tcttgaatca
    27241 gatttggttg tggatgaact agtcgtcatg atgcaaaaag aagtagctga tcgtatctct
    27301 gctgaaccag gaacgaaagc ttatggttca ttatcgattg ctgttcaata ttatatggaa
    27361 gccagtttag cattcatcgt accaaaaaca gtatttgtcc ctcagccaaa tgtagattca
    27421 gcgattttga aactgacaag aagagatata ccggcagtcg aagtgacaga cgaaaaagca
    27481 tttttccgat tgacaaaagc ggcatttcag caaagaagaa aaacgctttg gaataacttg
    27541 cagcattcgt atggcaaaga tgaccagaca aaagcatggt tagcaaaaag cttggaaaca
    27601 gctggaatcg atccaaaacg tcgtggggag actttgtcat tacaagaatt cgccgcatta
    27661 agcaatgcga tgtcagaaaa tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa
    27721 agctgtcctg catcaagtaa taagaacagc caaacaaaaa tagttcctca tgtttttcgt
    27781 ttgtttgggc ttgttaccgc agatgcaagc ttttgaacac cgtttaagaa agaggttgtg
    27841 ggcctgccgt ttaactctaa gtcacaagga acaatttcaa aaaacagctt ctcatgtttt
    27901 tgagttagtt ggtcttatta cttgatgcaa gcttttgacc actattatgc cgaacaagga
    27961 gatgtgacac aaattttgtc acatctccta tttttgtttc ttgatcaaaa aattcttata
    28021 cttgatactc acactcattt tgtatggatt tagaaaagaa tatgtgtctt gacagttatt
    28081 agtaagtgag ttatactaag tcgacattta tgtgtcaaga catattagga gagagaaaaa
    28141 atgagaaaaa attcagttcg aaaactaacg atttctgctt tattgattgg gatgggggtc
    28201 attatcccaa tggtcatgcc taaaatcatg attccaccag cttcctttac tttggcgtct
    28261 catgtgccat tatttattgc gatgtttttt acaccaggag ttgcagtggc tgttgcgcta
    28321 ggtacgacat ttggtttctt tttaacaacg ccggttatca tcgcattgcg agcattgtca
    28381 catcttgtgt ttgcgttgat tggggcttgg tacttgcaaa agcatccgag tatcgtttta
    28441 aaaaatggac agtttacttt tgctaattgg cgtttccaag ggttcaattt tgtgatcggt
    28501 gtgattcatt ctttagcaga aatgctagta gtcagcatct tttactttat tggtaatatg
    28561 cctgaaacat attattctca aggatatttt tatacagtat tcatcttgat ggggattggt
    28621 gggcttatcc acagtttagt tgattataat attgcttatt ttatagcagg aacactcagc
    28681 aaatattttg atatccccgt atttacggca gcaaaaaaag aaaatcggaa agagtcgatt
    28741 caagggtacg cgaagaaaac gacatattag acatgatctc tcaaaaaaaa aactctcatc
    28801 tagtaatgga taataaatcc tctaagatga gagttttttg tcctaaaaag agctgcacca
    28861 acatcttctg gcacagctct aacagggggc aatttatgga agtttgatga cttgttctgt
    28921 ttctttatca aagaagtggc ctttgctgat gttgaaggca aggtcgacta tttcgcctgg
    28981 tttatggaaa tctcttgcat ctacttttga gatgaactca gtagaaccag tttttgtgta
    29041 aagcatcgtt tcagctccaa gtaattcgga aacgacaact tctgatttca ctacggcttc
    29101 aggtgaagct tcaagggcta cttgttcact gtggatgtct tctggacgaa taccgaagat
    29161 aacttctttt ccttcataac ctttttcaac taatactttg ttttttcctt caggaatacg
    29221 aagcttaagc ccatgtccat ctgtgataac accatttttc aatgtcacat taaagaagtt
    29281 catggcagga gatccaataa agccggcaac gaacatattg acgggtgtat cataaacttc
    29341 tttcggtgta ccgatttgtt ggataaatcc gtctttcatg ataacgattc ggtcagccat
    29401 agtcattgct tctgtctgat cgtgagtaac atagatggtc gttgtttcaa gacgctggtg
    29461 aagtttggca atctcagcac gcatggctac acgtaattta gcatctaaat ttgataatgg
    29521 ctcatccatt aagaagactt ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg
    29581 gcgttgtcca ccagataaag cagctggttt tcgtttcaag tattctgtca gacctaagat
    29641 ctctgctgca ttgtccacac gttttttgat gtctgcttta tcatatttac gtaatttcaa
    29701 gccaaaagcc atattatcaa aaacagtcat atgaggatac aatgcgtagt tttggaaaac
    29761 catggcaata tcacggtctt taggtgccac gtcattcatg acttttccgc cgatatttag
    29821 ttctccttca gagatatctt ccagtcccgc gatcattcgc aaagtagttg actttccaca
    29881 accggaagga ccaacaaaaa cgataaattc acggtctttg attgatagat taaaatcagt
    29941 tacagaataa ttttctgcat tgtcatattt tttatatacg tttttcagag ccatttctac
    30001 cataatacgt tccacacttt cttttttagt tttattaact atttaaagta taaatgaaag
    30061 cgttaactaa cttcaatgtc atgttgaaca agaatttttt cttttttttg tcaatgtgct
    30121 agttttaagc tttaataaat atccataaga aataaaaact gatacactgg agtaaataag
    30181 tatgttaaca atatcaaaag gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg
    30241 aaaaaaacct atgatggtga aattagcaac agcgtataaa acaatattgg aaaaacatga
    30301 actttttgct accgggacga ctgggcagag gatcacggaa gcaaccggct tgccgatcca
    30361 tcggtttaaa tccggaccgt taggcgggga tcagcaaatt ggagccatga tttcggagga
    30421 tcagttagat ttggtgattt ttcttcgaga cccgctggct gcacagcccc atgaaccaga
    30481 tgtaacagca cttatccgtt tatctgatgt ctatgagatt ccgttagcta cgaatatcgg
    30541 aacagctgag gtgctactaa gaggactgga agccggcttc ctagattttc gaagaatcgt
    30601 ccatgaaata gacaagcatc cactttcatt ttgatggata agcgtatgga aaaataacgt
    30661 ttaatgttcg cctatatgta gctataaaag ctaaacaaac tgataatatg cgatataata
    30721 cgaacgtatt ggataatata aacaataata ttagtttttc gtatttgcag taaatgtgaa
    30781 aatatgctat agtttctgtg ggaaaagatc ccaaaaataa tacagagtag aaaataaagc
    30841 gttaagtgct aagaggatgg gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt
    30901 tattttcgca ttcgctgcat gatacgatgt agcagctctt aaaggagatg ctaaaaatga
    30961 agaaacaccg gttagacgca cgtatgattg ctattatggg cttattgatt gcattgatgg
    31021 tcactttgtc ccgactggta gcaatcgaga ccccatttat taaaatcagt gtcacattta
    31081 ttccacaagt gatcatgggc attctttttg gtcctttttg gtcagggata ggcgcagttt
    31141 tagctgattt agtaggaatg gccctcttct cgaaatctgc attctttatt ggatttacat
    31201 tgaatgcatt tattgaagga gcaatctacg gattcttttt ctatcgaaaa gagatcactt
    31261 ggaaaaatgc cattctggct acgcttagtg tcacgctgat catcaactta tttttgacac
    31321 cattatggct tgcgttgatg taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc
    31381 gcctgttaaa aacagtaata tggttaccga ttcaatcaat tgccatttat tacgtaggac
    31441 gtagtattcc ttacaaaaaa attttaagaa gtttagcgat ccacgctaaa taaaaaacaa
    31501 gcagtcttcc catcccagaa gactgcttgt ttttattgtg ctgtgaattg tgcttgatag
    31561 agccgtcggt aataaccatc tttttgaagc agctcatcat gggtgcccat ttcgacaata
    31621 gctccctgat ccatcactaa tatctggtcc gcatttttga ttgtggacag gcgatgggca
    31681 atcacaaaac tggtttttcc ttccatcatt gctaagaagg cttgttggat ttttttctct
    31741 gtcaatgtat cgactgagct ggtagcttca tctaagatca acataggggg attactgatc
    31801 atcgttcgag caatcgtcaa cagttggcgt tgtccatctg atatctttaa cccttgaccg
    31861 ccaatcttag tatctaattt ttccggcaaa cgttctacaa attcatacat ataagaggct
    31921 tttagggcat cattgatttc tgcgtcactt gcttccggat tcccaaaacg aagattttct
    31981 cgcaatgtac tgtcaaatag ccaagtatct tgtaacacca ttccaaaact tttacgtaat
    32041 gtatcacgtt ggatagagcg gatgtcatgg tcatcgactg taattgttcc ttttgttgtg
    32101 tcataaaaac gcatcagcaa attaacaagt gtggattttc cggcccctgt ttttcctact
    32161 atagcaatgg tttgacctgg ataagcagta aaattgaaat cttcgatcaa acgctgcttg
    32221 gggtcataag aaaaagaaac atgatcaaaa gagacttttc ctttgatatt tttgggttcc
    32281 aaggcatagg catctggcaa atcaggcttt tcttctggct gatcgatcaa atcaaatgct
    32341 cgttctagac cagaaaaagc ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat
    32401 ggtttagaaa actgacttga gtagatcgta aagctggaaa tcacaccaac agtgattgac
    32461 gaaccactct tcaatgccaa taggccgcca actaaaccaa tcgataaata agcaaggtga
    32521 tctacaaaac gagaagacgg gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt
    32581 tcatatagat cagcatttag cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa
    32641 gctttcacga ttttctggtt accgattcgt tcggtcacaa agcccgagat atccccaata
    32701 atcctttgct gtctatcgaa atttgtttgt gaagcacgag caacgatcca actcacaagg
    32761 aaaataattg gtgtagaaca aagaacgacc aatgtcagaa gtggacttag acgcatcata
    32821 aaaataaacg aaagaatgat gattgccact ccagaaaata attgattgaa ggccgcagaa
    32881 attgcaatgg aaatattgtc catatcattt gtaaatcgac tgacgatatt tccgtgggag
    32941 ttttgatcat aataacgaag tggcaatcga ttcagatgag caaatgcatc ttttcttaat
    33001 tttgcaacgg ataagtaagc taaccgattg cttaacactt ggatgagcca ttgactgact
    33061 acgttgatta aaacgatacc accgaataga ataagtaaat ggaacagtcg attgaagcgg
    33121 acctgtcctt ctccaatcat ttgatcgata ctttgaccaa tttgtaacgt catcacaact
    33181 gttgcaatcc cattgatgat tcctagtatc acagctagcc aaatgtcttt tgcataggca
    33241 gtaaggtaag gaatgaatcg gcggagcgtc tgcaaggata atttttttac tttcataact
    33301 attcttcctc ctgtgatgcg acgatttcct gatattctgc tgattgagca agtaattctt
    33361 catgggtacc ctgagcagca atcttacctt gatccatcac taagatatga tgtgcttctt
    33421 gaatagagcg gacacgttgg gagataataa tcactgtact gccaagataa gtctgtagcg
    33481 ccttacgcaa atccaagtct gtttgataat ccaacgcact taaagagtca tctaaaatca
    33541 gtaatgctgg cttggcaatc aaagctcttg caatcgtcag tcgctgtttt tgtccgccag
    33601 agaaattttt cccattttcc atgacttttg tatctaaacc atttggaagc tgacggacaa
    33661 attcttctgc ctgagcgatt ttcagagctt cccaacattc tttttctgtg gcgtctttct
    33721 taccccattg aaggttttca cgaatagtcc cggtaaatag tacagaagtt tggggaacaa
    33781 gagcaatttg ctgacggaga tgatccagct gccaagaacg aacatccatt ccgtccatta
    33841 taactgtacc ggaactgaca tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc
    33901 cgcttccggt tggaccaatg attccaagga ctgtatttcg cttcagctga aatgtaatat
    33961 tttggagaga aagaccagaa tcaggagtat aacggaagtc aacatggtca aaagcgataa
    34021 ttgcttcaga agaaaaatcg ggagtagtac tgacctcagc ttcaagaatc gaattttctg
    34081 tgtctagcac ctctttcacc cgatttcctg aagcttcggc acgtgtgaag atcacgacga
    34141 ggtttgaaac aacgatcagt gccaaaagca tttgattcat ataattgatc aaagcaagaa
    34201 cttgtccttg ttgcaagcta ccaatattta ctttgatccc gccaactgtc agcaaacaga
    34261 tgatcccgac attcatgatc aaggttgtag caggtgataa tagggcagag atattggaaa
    34321 cacgtaaata attgttcgct agatcatttg tgcttttatc gaacttctct gtttcttttt
    34381 ttgtctgcgc aaatgcgcgg atgacacgaa caccgctaag attctgacta acgtgttcat
    34441 tgagctgatc tagtttttct tgcacttttt ggtagagagg aacggttttt ttgatgataa
    34501 aaaataaaat gattgagaaa ataggcaata atgctaagaa aaacagacct acttcccagt
    34561 cgatcacaaa agccatgacc actgacccga tacttaaaaa cggtgctctg atcaataaac
    34621 gaatgaccat tgctaaagct agctgaagct gattgatgtc gtttgtcatt cgggtgatca
    34681 aggtatctgt tccaaaatga ttcaactcgt tgtgagagaa agtattgatt tttttgatta
    34741 gctgattacg taactctgta ccaaatcctt gtgaagctac tgaagcatag tattggcaaa
    34801 tgatagcaca aatcagacca atcacagaca tagcgaccat ccataagccc atttcaatga
    34861 tttttcctcg gtcattcatt tttagtccat tatcgatcaa ggaagccatc attaaaggca
    34921 ggacaagttc gaagacagct tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac
    34981 gatagtcttt tgcataacgt aataactgaa tcatatgatt cctcctatta tttatttgtc
    35041 cacaattgaa aaaacaagta ctaccacatt gtagcttagg aaatttagaa tgaaaagaga
    35101 acaagccttt tcataaatga aagttttatt atgaagaagc tttagaaatt gaatagacta
    35161 ttttttttaa aaaaaagaaa
//
LOCUS       DACDMM010000010        34702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDMM010000010 DACDMM010000000
VERSION     DACDMM010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.010"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            193..1899
                     /locus_tag="IWD16_000380"
     CDS             193..1899
                     /locus_tag="IWD16_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP9087466.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2057..2959)
                     /locus_tag="IWD16_000381"
     CDS             complement(2057..2959)
                     /locus_tag="IWD16_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9087467.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2956..3660)
                     /locus_tag="IWD16_000382"
     CDS             complement(2956..3660)
                     /locus_tag="IWD16_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP9087468.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3691..4332)
                     /locus_tag="IWD16_000383"
     CDS             complement(3691..4332)
                     /locus_tag="IWD16_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9087469.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4370..4639)
                     /locus_tag="IWD16_000384"
     CDS             complement(4370..4639)
                     /locus_tag="IWD16_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087470.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            complement(4612..5301)
                     /locus_tag="IWD16_000385"
     CDS             complement(4612..5301)
                     /locus_tag="IWD16_000385"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP9087471.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            complement(5308..6024)
                     /gene="pyrF"
                     /locus_tag="IWD16_000386"
     CDS             complement(5308..6024)
                     /gene="pyrF"
                     /locus_tag="IWD16_000386"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP9087472.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(6021..6953)
                     /locus_tag="IWD16_000387"
     CDS             complement(6021..6953)
                     /locus_tag="IWD16_000387"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP9087473.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6950..7726)
                     /locus_tag="IWD16_000388"
     CDS             complement(6950..7726)
                     /locus_tag="IWD16_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP9087474.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7780..10965)
                     /gene="carB"
                     /locus_tag="IWD16_000389"
     CDS             complement(7780..10965)
                     /gene="carB"
                     /locus_tag="IWD16_000389"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP9087475.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10970..12046)
                     /gene="carA"
                     /locus_tag="IWD16_000390"
     CDS             complement(10970..12046)
                     /gene="carA"
                     /locus_tag="IWD16_000390"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP9087476.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12051..13331)
                     /locus_tag="IWD16_000391"
     CDS             complement(12051..13331)
                     /locus_tag="IWD16_000391"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP9087477.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13350..14276)
                     /locus_tag="IWD16_000392"
     CDS             complement(13350..14276)
                     /locus_tag="IWD16_000392"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP9087478.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14289..15572)
                     /locus_tag="IWD16_000393"
     CDS             complement(14289..15572)
                     /locus_tag="IWD16_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP9087479.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15586..16125)
                     /gene="pyrR"
                     /locus_tag="IWD16_000394"
     CDS             complement(15586..16125)
                     /gene="pyrR"
                     /locus_tag="IWD16_000394"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP9087480.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16360..17271)
                     /locus_tag="IWD16_000395"
     CDS             complement(16360..17271)
                     /locus_tag="IWD16_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9087481.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17268..17732)
                     /locus_tag="IWD16_000396"
     CDS             complement(17268..17732)
                     /locus_tag="IWD16_000396"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP9087482.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17737..18435)
                     /locus_tag="IWD16_000397"
     CDS             complement(17737..18435)
                     /locus_tag="IWD16_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9087483.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18422..18808)
                     /locus_tag="IWD16_000398"
     CDS             complement(18422..18808)
                     /locus_tag="IWD16_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087484.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18955..20625)
                     /locus_tag="IWD16_000399"
     CDS             complement(18955..20625)
                     /locus_tag="IWD16_000399"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP9087485.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20808..21041
                     /locus_tag="IWD16_000400"
     CDS             20808..21041
                     /locus_tag="IWD16_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP9087486.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21140..21571)
                     /locus_tag="IWD16_000401"
     CDS             complement(21140..21571)
                     /locus_tag="IWD16_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP9087487.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21650..22063
                     /locus_tag="IWD16_000402"
     CDS             21650..22063
                     /locus_tag="IWD16_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP9087488.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22101..23249)
                     /locus_tag="IWD16_000403"
     CDS             complement(22101..23249)
                     /locus_tag="IWD16_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9087489.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23371..23967)
                     /locus_tag="IWD16_000404"
     CDS             complement(23371..23967)
                     /locus_tag="IWD16_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9087490.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24167..26809)
                     /gene="alaS"
                     /locus_tag="IWD16_000405"
     CDS             complement(24167..26809)
                     /gene="alaS"
                     /locus_tag="IWD16_000405"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP9087491.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27197..27628
                     /locus_tag="IWD16_000406"
     CDS             27197..27628
                     /locus_tag="IWD16_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9087492.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27673..29022)
                     /locus_tag="IWD16_000407"
     CDS             complement(27673..29022)
                     /locus_tag="IWD16_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9087493.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29092..30255)
                     /locus_tag="IWD16_000408"
     CDS             complement(29092..30255)
                     /locus_tag="IWD16_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087494.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30384..30707
                     /locus_tag="IWD16_000409"
     CDS             30384..30707
                     /locus_tag="IWD16_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087495.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30890..32239)
                     /locus_tag="IWD16_000410"
     CDS             complement(30890..32239)
                     /locus_tag="IWD16_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9087496.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32308..33261)
                     /locus_tag="IWD16_000411"
     CDS             complement(32308..33261)
                     /locus_tag="IWD16_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP9087497.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33363..34553)
                     /locus_tag="IWD16_000412"
     CDS             complement(33363..34553)
                     /locus_tag="IWD16_000412"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP9087498.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9856 a         7599 c         6093 g        11154 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgatacc taaaaatttg gatttcaacg
       61 atagaaacac tgttttttat gtaattagtc caagattttt ctagactttt gctttactat
      121 tcggccaaat tggtttttct tctttttttc gttataatga atatacttat gaaaagaaat
      181 gaggaatgaa ccatgtcctt tgatggtgta tttacccacg cgatggtcaa cgaattgcgt
      241 gaaacgttac tatctggccg gatctcgaaa atccatcagc cttatgaaaa tgaaatcgtg
      301 ctggtgattc gttcacgcgg caaaaaccat cgattattat tatctgcgca cccaagttat
      361 gctcgtgtac agattaccca aatcgattat cagaatcctg ataatccgcc taacttcgtg
      421 atgatgcttc gaaaatattt ggatggcgct attttagaag acatcgaaca aatcgaaaat
      481 gatcgagtga tccatttcca ctttgctaaa cgaaacgagt tgggtgatct acaaaatatc
      541 atattgatcg ttgaattaat gggacgtcat agtacgatcg tcttagttaa tcgagaaact
      601 ggaaaaatac tggatgcgat caaacacatt ggaagctctc aaaacactta tcgttctttg
      661 cttcctggtg tagaatatgt tgctccgcca aaacaagagg tcttgaaccc atttagctcc
      721 gagaaagaaa aaatctttca gcggctttcc caaactgaac tagatccaaa agcaatccaa
      781 cgtcagtttc agggaatcgg tttcgatatc gctcaagagc tgaccaaaag attactggaa
      841 cgtcctaatg aaaagatggt tgtatgggac gaatttttct cagccatcag ccatcagcct
      901 attcctactt tttacgaaac ggaaaataaa gattttttca caccgattgc ttatcaggta
      961 ttgtccgaac aggcatctgc tgtaacagct tatccaacac tcagtcaatt attagatagc
     1021 tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac aaggcggaga actgattcga
     1081 aaaatcgaaa atgaactaaa acgaaataaa aataaattga aaaaacgtga acaaacactc
     1141 aaagaatccg aaaacgctga gaattaccgt agaaatggcg aactacttac tacttttctg
     1201 acacaagtac cacgcggcgc aaaagaagtt gttttaccta attattatga agaagatcgg
     1261 ccaatcaaaa tagcactaga tccggcattg acaccaaatc aaaatgcaca gaaatatttc
     1321 catcgatacc aaaagctgaa gaacgctgtt aagctgattg gcgaacaaat ccaagaagcc
     1381 aaagatgaaa tccaatatct tgaatctgtc ttatctcaac tggaaattgc cggcccaatg
     1441 gatatcgaag caattaaaga agaattgact tcagaaggat acctgaaaaa gaagaactta
     1501 aaaaaacaaa aacgtaagaa accatctcag cctgatcaat acttttcttc tgacggaacg
     1561 ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc tgacaatgaa gacagctaaa
     1621 aaaaccgact attggcttca tgcaaaaaat atacccggtt cgcacgtcat cattaaaagt
     1681 gacaagccct cagatgaaac aattacagaa gctgccgagt tagctgccta tttttcaaaa
     1741 taccgatact ctgcccaagt gccggttgat cttgtacagg taaagcatat ccgaaaacca
     1801 aatggcgcca agcctggtta tgttatttat gaaaaccaaa agacgattat cgtgacacca
     1861 gaagaagaaa aaattacagc tatgaaacaa aacaggtaat caatgagtgt ttctagaatc
     1921 atatttgcgt actttcccac aacagcaatt agttataaat tgtttgctac aaaaagagga
     1981 gttgtgacaa aattctgtca caacccctct tttttgatca acggtgttca ataaacagcg
     2041 ttctcatctg cttttcttag ctctgctggt ccagtaaccg agtaagctct ttgataaatc
     2101 tttgagcgat ttcactgagc ggacgtttcg ccgaataaat gatataaaat tcatttagtt
     2161 cgccttctgc caaagggatt aaccgcaagt gctcgcgaat ccctccaatc acaataccag
     2221 atcctgaagc gtaagcatca gtttcaagaa cgatattcat caaagtacct cgatcactcg
     2281 tataaatcac tgtttcttga tcagggatct cgataaggtc ttcataaaaa tacgggaaat
     2341 tatttccatc ctgtgtaaaa cgaacttgag gataagataa caattcttct ttggtgatct
     2401 ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat atgcgttttg aattttccaa
     2461 tgctgcggcc acttagctct ccttgttgta aatatcttct gataccagct tgattatgct
     2521 cattcatata aataatgccg atctcactgt gctgctcttt gacttcttcg atcactctcg
     2581 ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc tttgaattcc tcgagtagct
     2641 gtgcggtcaa aggacctaag aaatcataat gctgggcagc gatcgaaaaa ttgtctctct
     2701 tttcactcgt tagatagtga gtttctaatg tatcaagctg gaccaatatt ttctcggcac
     2761 gatttaagaa gtcttgccct tcttctgtta aatatgcccc tcgattcgtt cgagtaaaca
     2821 gcgtgatccc taactcggtt tctaattctt tgataccagc tgaaagactt ggctgggaga
     2881 caaacatttt ctttgccgct tctctgaaac taccattgtt tgctacagcg accacgtatt
     2941 tcatctgctg aatattcatt gctcaaactc ctcgaaataa taaatttctc ttccattcaa
     3001 gggctggatt ggtcggttgt tcggtcgagc atactgacca tcgaagggga gaagaaactc
     3061 ttggttcagt tttcctacat gatccattac aaaccattgg attggcccgc tggtaggcgg
     3121 agttgtcaat gagccaatat aatggaaatg gcttttttgc tccggaaaga acacatccgg
     3181 atttacccaa tgctcatgtt tttctaagtt ccaactttcc ccattttctt gattatacag
     3241 ccaaccttcc tgtaccatta gctggtacat gacacccaca actagattct cttttttgct
     3301 gttcatatga acaaaatgaa attcaatatc ttcctgtttt ccgttgatga tatgttcaga
     3361 aggcaagtgg aaatgaatat ctgtcaaata atagcgttct ttcttgaact ctacataact
     3421 gagttggtca aaaggtacta aatgaatcgt gtttttgaat tccttgtcag taaaccgttg
     3481 cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg gtttcttccc ccttggtcaa
     3541 atgaataggc gactgcaatg gaaattccgc acctctcgca aaccaatcac ataaactcgc
     3601 ccaattttcc ggacctttgt ctccagaata gccccattcg acatccatat tctttttcaa
     3661 ctatacttcc ccctgcatct catatcattt ttatttttct tctttcttta caatcgttga
     3721 gacggtttct aaaaacgcct cttttccata aaattcctgg ttcaatagtt ccggaaatat
     3781 ctctttaaag aaatactgaa cagaaggccg atgttcaaag cttttaatcg tagtcactgc
     3841 ctcaatgaac atctccttat aaactttttc cgggttattt ttgatgattt catcaaaaga
     3901 ttcataaaga aggtcaacct tatctgaaat ggctaatagt tctccttcta cggtttcatc
     3961 ttttccttcg aacaaccgac ggcgataaac ttcttggaac tcagctggaa tctcttggtt
     4021 gatgaattcg tccgtcatct tttcttcaac tgtttgcagc atccctctta actctttgct
     4081 tgcgtatttc accggcgttt tgatatcacc aataaaacgc tctgtgtagt catgattcaa
     4141 tgatttttca tagagcagtt tccaatcgat cggttgtcca ttcgcttctt caatgtctcc
     4201 tagaacttgc gcaatagatg ctacacgata cgaatgtgct gcaactgtat gctctgtata
     4261 tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg ttcaatccta aaataaattc
     4321 attcaacccc ataaaagtta cctccctttg agatttcttt ttccgaatat catatcatta
     4381 aataactgat attcgcaaca tttccgctct ttaccatttt tgaaacagct cccttaccaa
     4441 aaaatagaga aaatctatat ataatagaaa aaagagaaga gattattctc ttctttctgc
     4501 taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa ctgatcacga tagaagtttc
     4561 ttttcatacc tgatgaaaag atgcttctcg caatcagttt ttggattttt gctattccaa
     4621 ttgatgattc tggagacaca aatcggattt ctctatcttc atcaagcgcg cttcaatctg
     4681 ccaattttca ggatcttttt tccattcttt tagcagagtt aattcttgct catcgatata
     4741 acgatcttct aaagctgctt caatcaatac agaataattg gtcaacgtca taagagggat
     4801 ttcggctttt tcgaaatttg cttttccttt tggcaactca tacgtaaaga tagccgcaac
     4861 accaaggata tccgcacctt ctctttttgc tgcttcagct gcttccaaga cacttccgcc
     4921 tgtagaaatc aaatcctcga tcacaaccat cttctgacct tcaaatatac gtccttcgat
     4981 ttggttacct ttcccatgat ctttcgcctt acttcgaata tagaccattg gcaaatccag
     5041 tatttccgct acccatgcgg catgagggat tcctgctgtt gcagttccag caatcacttg
     5101 tacttcaggg taagcttctt tgattttgct tgctaatcct tttgcgatct cttttcggac
     5161 ttttggatag ctcatcgtga ttcggttatc acagtaaatt gggcttttga ttccacttgc
     5221 ccatgtaaaa ggattacttg gatttaaaaa tactgcttca atttctaata agtcttttgc
     5281 gatactttgt tcaacattca ttttaggtta gctccattct gtttgaatcg tttgatatgc
     5341 tttataagga ttagttgact gagtgattgg tcgacctata acgataaaag ttgagccgat
     5401 cttttttgcc tgagaaggtg tgactactct tgtctgatct cctgcgactg ttccttctgg
     5461 tcttataccc ggagttaagc aaacaaattc gtctcctgcc atttctttga tcgcttctac
     5521 ttcccaagca gaagaaacca caccatctaa tcctgctttt ttggcacagc tagcataatg
     5581 gatcacactt ttttctaaag aaacttcgat tagttgatcg gcatgcattt cttcttcact
     5641 tgtagaagta agctgagtga tagctagaag tagcggacgt tttccacctt ctggagtccc
     5701 ctcttctaat cctcgcattg ccgcctccat catacgaata ccgccagcgg catgaacaca
     5761 ggtgagatca acaccaagct ttgccaatcc ccgcattgct ttttcgaccg tattcggaat
     5821 atcgtgaagt ttcagatcta aaaatacatt atgaccagcc tcttttagat aacgtacgat
     5881 ttcaggtcct tcttggtaga acaattccat tccgattttc acaaacaatt tttcttcttt
     5941 cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt gaaaaatcta atgcaataat
     6001 tggtcgttgg tctagttgat tcatcgttca cgttcctctc tcacttcttt gattaatctt
     6061 tccagcgatt cgatgcctag ttcgctcatt cgcacaggga gctctttaat tagtttagga
     6121 cagatatacg gatcggtgaa attcgcggtc cctatagcta cagcacttgc tccagccata
     6181 aacatctcaa gaacatcatc cactgtctgc acgccgccca tcccaatgat tggaaggctg
     6241 gatatacttg ctacttggtg aatcaaacga atagcgacag gcttgattgc tggtccggag
     6301 agtccacctg tttgattggc taaaatcggt tttcttgtct taaggtcaat ccgcatacca
     6361 agaagagtat tgatcatcgt aaacccatct gcccctccag cttcgattgc ctgagcgatt
     6421 ggaacgatgt ctgttacatt tggggacaat ttgacataga caggaacaga agaaactttc
     6481 tttactgctt gtgttaattg aaaagcaaca tcagaatctg ttccaaaagc aattccgcca
     6541 tgtttgacat ttgggcaaga aatgttcagc tcgattgctt tcacatttgg tgcttcgcca
     6601 attttgctac aaacttctac atagtcttct tcacaagcac cagcaacatt tgcgatgatg
     6661 ggtaactcat tgtactcctc taattctgga agaagtttat gcataacaac gtctagacca
     6721 ggattttgca aaccaattgc attcaacatg ccagatggtg tttccgccac tcttggcgtg
     6781 ggattgccaa agcgggcatt tggagtcgtt gccttgacca tgatcgatcc aagctgattc
     6841 aaatcatagt attttccgta ttctttgcca aaaccgaaac agccgcttgc cggcatgata
     6901 ggatttttta attctaggcc aggaagtttg accgctaaag gattctcatt catataatta
     6961 cctctcctat tttaaaaatc ggtccttcgt cacaaacttt cacacttttt gttcctgttt
     7021 catcttccgg cgtatgacaa acgcaagcat agcaagcccc tatcccacat gccatgcggg
     7081 attctaatga taactggaca ttttgatgcg aagaaaacag ctgttccact gtcttcaata
     7141 acccgtttga accacatgca aaaacagctg taggttccgt cttcgttcct aacaataaat
     7201 taccgacatt tccgtgcata ccgtatgttc catcgtcagt agcgattctt gtttctccta
     7261 gacgattaaa ttctttttca taataaacga cttcatgtgt ggcaaaacct aaaaaatgcg
     7321 ttggcttaat tcctttttcg actaactgtt ttgataactc atatagagga gggacaccga
     7381 ttcctccacc gattataaat acttcgtctc ctgtatgaag attggacaaa tcaaaaccat
     7441 tgcctaaagg acccatgaca tctaaatggt ctccggcaga aagagtagag aagactctag
     7501 tcccctcacc ttccacccga taaatgatac ggcaagtatt tgtttcgtgg tcgatatgat
     7561 tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg cacgagaata tgcaagaact
     7621 gtcctggttg ctgcatttct tttactagat cacctttcaa tgtcatttca aaaatctttg
     7681 gtgccagctg tttttgctga acgatttcca tcatttcttg tttcatctgt cccctcctgc
     7741 aacggttttt cgtctttttg tcttaacatg tgctcgccgt taaatagggt tagttgaaaa
     7801 agcacgggat tccatcactc gaacgatcgc atctgctgtg tctaaagaag taaacaatgg
     7861 aataccatgt tcaactgctt ctcttcggat gatgaaaccg tctttagaag catttccccg
     7921 atctttatca atcgtattga caaccacttg tgttttacca tctcttatta agtcgactac
     7981 atttttttct gctgcttcct cagaaatttt cgctactgtt tcaacgacca gaccattttt
     8041 ttcgaaataa gcagctgtat gtttcgttcc tatcagacta tagccaattt ctgtaaaacg
     8101 tttagctaat tctagtgctt cttcttttgt ttcgtccgca atcgtaaata atacggctcc
     8161 atattcaggc aagtgtaatc cagaagcttc gaatgccttg tacaaagctt tgtctaattg
     8221 tctgtcagat cccattactt ctccagtcga tttcatttca ggccctaagt aagtatcaac
     8281 tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca tgaacttctt ttgtttctgg
     8341 ataaagtccg ttttcataac caagtgtctt tagtttctct cccaaaatag ctttcgtcgc
     8401 aatttgtgcc attggaattc ctgtcacctt acttaagaat ggaacagtcc gacttgctcg
     8461 aggattgact tcgatcacat aaacctcatt ttcatggatg acaaactgga tattcatcat
     8521 cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa tcttcgatcg ttttcttgat
     8581 cgattcagat aatgtttgag gtggatacac agccattgaa tcccctgaat ggacaccagc
     8641 acgctcaata tgctccatga tccctgggat gagtactgtt tcaccgtcac aaatcgcatc
     8701 cacttcgcat tcaccaccaa gcaagtaacg gtccactagt actggatgtt ctggagatgc
     8761 tttcacagca tgttccatgt aatcttctaa atctcgctgg ttttcaacga tttccatcgc
     8821 acgtccgcct aatacatagc ttggacgaac aagaactgga taaccaattc tcgaagcgat
     8881 aaccacggct tcttctttac ttgtagcagt atcccctaaa ggttgaggaa tatccaactc
     8941 tttcagcgct tgctcgaata aatcacgatt ttccgcacga tccaagtctt cgatcgttgt
     9001 ccctaagatt tttacaccag cagcagtcaa aggttcggct aagttgattg ctgtctgacc
     9061 accaaattga acgattactc ctatcggctg ttctagctcg atcacattca tcacatcttc
     9121 gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa aagtcagtcg aaactgtttc
     9181 aggattgctg ttcatgatga ttgcttcgta tcctgcctgc tggattgctt tgacagaatg
     9241 cacagtcgca tagtcaaatt caaccccttg tccgatacgg ataggaccag atcccagtac
     9301 tagaattgag ggcttttctg atcgtgaact ttcattttca tattcatagg aactataaaa
     9361 ataaggtgtt tttgattcga attccgctgc acaagtgtca accatcttat atacaggaat
     9421 caaatcattt tcttttctca tctggcgaac ggcttcttcg tttatttccc acagctccgc
     9481 cacttttttg tctgtaaaac cgttttgttt cgctgttttc aatatcttgt catccaacgg
     9541 ctgctgtttc aactgctgtt cgatttctac gatatgcaac agtttatcta aaaagaataa
     9601 atcgattttt gtcaaatccg ctaattcttc tatggtgtac cctcttctaa tagcttcagc
     9661 tacataaaac aggcgatcat cttgtgctcg aaccactttt tggattaggt catcgtccgt
     9721 gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg atttctaaag aacgcacagc
     9781 tttcaataaa gactcttcga tattccgacc gatagccatc acttcaccag ttgctttcat
     9841 ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca aacggccagc gaggaatttt
     9901 agctacaaca taatctaatg caggttcaaa ttctgcaaaa gtcgttcctg taacaggatt
     9961 tttcatttcg tctaatgtta atccaagcgc tattttagca gctagcttag cgatcggata
    10021 gccagtcgcc ttacttgcta acgcagatga acgagaaaca cgcggattga cttcaatcac
    10081 ataataatta aaactatgtg gatcaagagc gagttggaca ttacagcctc cctcgatctt
    10141 aagtgctcga ataatcgaaa gagaagcatc acgaagcatt tgatattcat ggtcagacaa
    10201 tgtttgacta ggggcaaata cgatagaatc acctgtatgg atacccaccg ggtcaaaatt
    10261 ttccatattg cagacaacga ttgcattatc agccgaatct ctcatcactt catattcaat
    10321 ttctttaaaa cctgcaatac ttcgttcaat caaacattgg gtagcggggg aaagacttaa
    10381 tccattttct gatacgatac gtaattcttc ttcattttca cacatacctc caccagttcc
    10441 gcctaaagta aaggctggac gaacgatgac aggatagccg attttatttg caaaatctac
    10501 agcttcttct actgtattga cgatttcaga ctcggggatt ggctgattca attctttcat
    10561 caactgtttg aatagatcac gatcttctgc ttgatcgatc gcagaaagct ttgttcctag
    10621 aagttctatg ttcaattctt ctaaaatacc tgatgctgcc agctccattg ccatattgag
    10681 gcctgtctgt ccgcctaaag ttggaaggag agcatctgga cgctcttttc gaagaatcct
    10741 tgatacaaat tcaagtgtga tcggttcgat atatacttga tctgccactt ctttatccgt
    10801 catgatcgtc gcaggattag aatttactaa aaccacttca tatccttctt cttttaaggc
    10861 aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct tgaccaatga tgatcggacc
    10921 agatccaatc accataattt tcttgatatc tgtacgtttc ggcatctgtt tactgctcct
    10981 ttcctgcgtc catcatttcc ataaattcat caaataaatg tagtgcatca tgcggaccag
    11041 gggcagcatc tggatgaaac tgcacactga aagcgggata atctctgtgt ttgactcctt
    11101 ctactgttcc gtcatttact tctacatgag ttacgatgag tttttctgga tcaatcgtgg
    11161 cttcatcgac tgcatagcca tgattttgag aagtaaaatc aattcttccg gtcgctactt
    11221 ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat tttataggta tctgcaccgt
    11281 tggctaaagc aaataactgg tgaccgagac aaataccaaa aatcggtact tttccttgga
    11341 tttttttgat catttcgatt gcttctggca catcttttgg atcaccaggt ccattcgtca
    11401 gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc atctgtgtta tagggaagta
    11461 ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat actatgtttc aagccgaagt
    11521 caatgacaat gactttccga cctgttcctg gacttggata aggtttgtta gttgagactt
    11581 gctgcacttg attggttgct agaacggttg ctttcagctg atcaaatgca tgttcgaagg
    11641 aatctccagc atcaactata ctagctttca tcgttccgac ttcacggatt ttgcgagtaa
    11701 gggcgcgcgt atcgatacct gatattccag gaattccttt ttgtttcaaa aatgaatcta
    11761 gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg ttcttttact actactcctt
    11821 tgcatgttgg agaaatcgat tcataatcat ctctatttac cccataatta cccaccatag
    11881 gataggtaaa tgtaatgatt tgaccgttaa aactttgatc agtgatagtt tcttgataac
    11941 cagtcatact tgttgtaaaa acgatttctc cgaagacact agcatctgca ccaaaagcct
    12001 ttccttcgaa aactgttcca tcttctaata taagcaaacg ttccatactt ttattctccc
    12061 ttctgccaca caagttttcc gttgacaaaa gtcatttgtg tttcgccttt tactttccag
    12121 ccgataaaag gtgtattctc cccttttgat aaaaagtctt ctttatcgat cgtgcaagtt
    12181 tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa ccgttagcgt tcctgcattc
    12241 aaaccaaaaa tctcagccgg tttaactgcc aaccaattga tcacttgttc caacgtgaat
    12301 cgaccagttt ctacaaaatg agtatagatc agttgaaacg ctgtttcgct tcctacgata
    12361 ccaaatggtg atttcatgaa actttggctt ttttcttcta atccatgggg ggcatggtct
    12421 gtagcgatgc aatcgatcgt accgtcaagc aagccttcga tcaaagcttc tctgtcctcc
    12481 cgtcctctta gcggcgggtt cattttccaa aagccgaaat cttcgggaat atcttcgtcg
    12541 atcaaaataa gatgatgggg tgaaacttct gctgttacgt ggattcctgc tttttttgcg
    12601 tctctgatta cacggacact ttctttagta gaaacatggc aaacatggta atgaacacca
    12661 gttgcttcag ctagcaacag atctctagca atttgagaag actcggttac gcttaagatc
    12721 ccgggaagac caagttcttc tgccttcttg ccagcgtgca taacgccgcc aaataataaa
    12781 gattcatctt ctgtatgggc tactagggct ttattgtttt ttgcagcttc tttcatagcc
    12841 agatacatcg tcccagctgt ttggacacct acaccgtcat ttgtgaaagc aaaagcacct
    12901 tcttcaatca gtgcttcttg atcaactaat ttctcacttc gcagattttc tgtgattggc
    12961 gcatactgca ataccttcac aactgcatct tttctaatga tgtcatatac ttgtctcagc
    13021 ttttctgccg tatcaggtac aggatttaaa ttcggcatcg cgcatacagt agtgaaaccg
    13081 ccacgagcag ctgagcgagt cccagcttcg atcgtctcct tgtatgtaaa tcctggctct
    13141 ctcaaatgaa catggacgtc cactaaacca ggtgtgatca attgtccctt tgcatcaaac
    13201 acttcatcaa attcggcttc tgagaatcct gtaccgattg ctttgattcg cccatcttct
    13261 atccaaattt ctgctggtgt cgtcatattt tttcgtgtat tgatctgacc gtttttaatg
    13321 agtgttttca ttttttcatc ctcctataat tacgcttttc catttagcac tgcttctaag
    13381 attgccattc tgacgaatac tccattgctc atttgctgaa cgatccgcga ctgtaatcct
    13441 tccactaatg aatcagctaa ttctacatca cggttaactg gtgctgggtg cataatgatt
    13501 gcatgttttt gcaacttctt cgcacgctca actgttaacc catgttcctg atggtagcct
    13561 tcttttgaga aactttcttt tccatcatgg cgttcgtgtt gcacccgaag catcatcatg
    13621 acatcaactt gatctagtag ttcatccaat ggcatataat gtccgtacac ttcgaattca
    13681 tcgtcgtacc attctctagg acctgagaag aatacttgtg cgcctagacg ttttaacatc
    13741 tgcatattgg atttagctac gcgagagtga gtgatatcac ctacgatggc aactttcaga
    13801 ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat caagcaagca ttgggttgga
    13861 tgctgtccgc ttccgtctcc tccattgata atcgaacact ggattgtttt actttgtatc
    13921 agttcgtcat aatagttctc atcaccatga cggataaccg ctacgtctac gcctaatgct
    13981 gacatcgtca gtactgtatc gtatagtgtt tccccttttt ggacagagct tgtgcttgct
    14041 tcaaattcaa tcacttctag ccctagtttt ttctccgcta catcgaagct tttatgtgtg
    14101 cgagtactat tttcgaaaaa gaggtttgta gcaaagtact ggcgcttttg cggagtccaa
    14161 ttagcacctt gcttgaattc ctgccctctg cggatcaagc ccataacttc ttgatcagta
    14221 agtgcctcaa cggtcaataa atgctttaag ctgatacgtt ctgatttgat gatcataatg
    14281 tttcctcctt agttttcttc gcttcgtgaa gtttctggta agataagatt taaaatgatg
    14341 ccaagtacag ttgctaatgc cattgccgac aatgtaaatg tgcctacttc aaagacgagt
    14401 ccgccaattc ctactactaa gataactgaa gcaatcaaca gatttttctt tttatcaaaa
    14461 tcgattttgt tctcgattaa aatcttcatt ccacttgcgg caattactcc aaataagata
    14521 aaactgattc ctgagatgac tggtccaggg atacttaaaa tcaaagcgct taattttcct
    14581 acgaatccta aaatgactgc aaatactgca gctccaccga tcacaaacac gctatggaca
    14641 cgtgtgatag ccagaacacc aatgttttca ccgtaacttg ttacaggagg acctccgaca
    14701 aaggctgcca cgatttgtgc taatccatca cctgtcaacg tcttatgaag acccggttct
    14761 tcaaagaaat tacgtttagt tagtttattc aataccataa gatggccgat atgctctgtc
    14821 atcgtcacaa aagcgatggg tgccattgtt gtgattgctc caagatatag ctttggttca
    14881 tattgtacaa agggaatctc aaagttagga acagcaaacc aggcagcgtc tacgattggc
    14941 ttagtatcca cgattccaaa cagcaaggct acgatgtatc cgctaataat acctagtaaa
    15001 atcggaatca atccgagaaa acctcttagc cacatgttga agaaaattgt taatcctaat
    15061 gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc cctgattata catagcattt
    15121 gttgctgcat ttgccgcaag tcccaacccg ataaccatga tgactggacc gacgacgatt
    15181 ggaggcaaga ttttatccag ccatgctgaa ccgatctttt tgatgatcag cgcaacaatc
    15241 aaatagacta atccagttgt gactgctcct tgtgcaatcg ctggataacc atctgttttc
    15301 atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc ctaagtacgc aggtatcttt
    15361 cctttagtag taaataagta caccaatgta cctagtcctg aactgactag cgcaatacct
    15421 ggatcgatac ctactaagat ggggactaac acggttgctc cgaacatcgt gaacaagtgt
    15481 tgtaaactta atcctaccca atgtcctggg gttgggcgat cgtggatatc taacaccacg
    15541 tcatcattat gaaatccttt tttctccgac atgtttctct ctcctctaat cttcctcttt
    15601 caaaatcatg atgcgatctt gtccatcaag ctcttgcatc tctactaaaa tctcttccgc
    15661 ccgagatgtt ggaatgttct tcccaacata atctgcacgg attggcaatt ctcgatgccc
    15721 acgatcgacc aggactgcta atgaaatttt tcttggtctg ccaaaatcca ttactgcgtc
    15781 cattgctgct cgaatcgttc gtcctgtata caaaacatca tcaatcaaaa taacttcttt
    15841 tccttctaag gaaactggga tgtcagaaga atgaagttcc ggttcttctg gattttcttt
    15901 tttgtcatct cggtataaag tgatatccag ttctcctaca ggaatatcga tatcttctag
    15961 ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt cctcgtgttt tgatacctac
    16021 caatacgata tcttggatac tatggtttct ctcgataatc tcataagtaa ttcgagtcaa
    16081 tgctcgcttc atggttacct gatctactac ttcttttgct tgcattttca ttccccctac
    16141 ttttttgcat aaaaaaactc ctgccctaag aggacaggag gaatgtttag actgagcgta
    16201 tactgataca ctgatagcta tactatgtca atgagcaatc tacttatggc gcaccatacg
    16261 tatccttgct cccacccttc ttagcctcac gggactaaac ttaaaggaat tctattaagt
    16321 tttatgcttt cgttgttaat tactataccg ttatctaaat taaaatgcaa tgttttttct
    16381 taatttttct aaagtttttt caaaaacttc tggaagaggt gcttcaaaga tcatcttttc
    16441 gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg agaaactgac cattgccttt
    16501 caaagttttc ttcggtccgt atactggatc tccagctaac ggataaccga tatacttcat
    16561 atgcacacgg atctggtgtg ttcttccggt ttctagtttt aattcaacca gagtaaatcc
    16621 ttcgaaacgt tccaatacag taaaatgtgt gacagccgac ttcccatctt ctcttatcgc
    16681 ctgcattttc cggttttcct tcgaacggcc gattggcgca ttgatggttc ctttttcgtg
    16741 aggaatctct ccatggacta aagcaacata tttgcgcaaa gaggtcttat cttttaattg
    16801 ctgagctaat gattcgtgtg cctggtcatt tttggcaacc attagcagtc cggaagtatc
    16861 tttgtcgatt cggtgaacaa tacctggacg aataacgccg ttgatcgtag aaagatcctt
    16921 gatatggtac atcagcgcat tgaccattgt gccatgcgta tgacctgctg aaggatgaac
    16981 gaccattcct tgaggtttgt ttatcaccgc tacatcttca tcttcgtaaa cgatcgtcaa
    17041 cgggatattt tctgggacca catctaatgt ttctggttcg gggatcaaga cagtaatctc
    17101 gtctccttgt tttactttgt aatttgcttt gactggctgt ccattgacag aaaccgctcc
    17161 atttttcagc cacagctgaa tttgcgaacg actgtaatcg gtgaataaat tagtcaatac
    17221 tttatcgatc ctaccagatt catggtgaat cgtcgtttta agttctgtca tttttttctt
    17281 tgatcctttt catctaaaat aatataaatt aacatacaaa tcactccaca tacaagtgtc
    17341 acatctgcta cgttaaaaat cggaaagctc ataaaatctg tctggaacat atctactaca
    17401 tagccaagac gaatacggtc gataaaattg ccaatcgcac cagctagaat caaactcaat
    17461 ccaatcatga accagcgatt acctttttca cgatttttaa tcatcaatgt gagaatcaca
    17521 acgactgcaa tgagtgtcac tatagtaaag aaccacatct tcccttctag catactccat
    17581 gcggctccag tgtttcgaat atgattcagt gacaaaatac ctggtataag atttttcgtc
    17641 tctccaagcg ctagattatc agtgatccac catttgacca actgatccac tcctactagc
    17701 aaagcgctga atatccaata aataattaac aaaaaatcaa tccttttcta tttgtttata
    17761 aatatcacga ggtcgtacga tcccgagcag attaccatcg ggattcccat ctttagtgat
    17821 aagtacggct tccaaagtcc cttgctcacg aaacatcgct tcaacctcgt aaatatgtgt
    17881 tttagctgga acaaaatgat aatttgtgta ctttccgttt tggatgatca aatccaacgc
    17941 tcgtttattt gttaaatcaa ccggaccttt ttggctttct tttgctagcc aaaacccgat
    18001 attacgtaaa gtaatcaatg ctacaaattt tcctttattg tataacggaa attgtgaata
    18061 ccttttttct gctactatct ttaacagttc aagtaacgga agatcttgtt caaaacctgt
    18121 tacacgtttt gcaaatcgag gcagcacggt ttcaggctga atcagttctt gttcgatcag
    18181 ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg acaaaatcga ctgcgatccg
    18241 atcatggacg atcgcattcc ttaactgagc aagctgtaaa agatcatctt cgtattttct
    18301 gatcattaat tgttttcttt gagctaggcg tctgacaagt tcggtaaatc ccatatttcg
    18361 tgcatttccc atctcgtctc tcatccattt ttcgattcga ttgaaactga ttaaaaaaag
    18421 atcagagttt gccataattt tcctccttga tcaaatatgc ccggtttttt atatagaaaa
    18481 agaacgcaat caacacaata actgcaaaaa aactgaaaaa taaattttgt aggaaaacgg
    18541 cccccaaccc agttgctaga aataaaaacg gatagatttg aagaatcaag taattcattg
    18601 tgatcacata agcaggcttt gcttctttca ctgtataaac ggggcttttt agccaataaa
    18661 aggtgacctg catcgtaaag ggcacttcca gatcaagcgt tatcttttgt tcatgactga
    18721 gaacactgcg tttttcattg ttgattctta gctgaatcga gctacccata ccgtaaaaac
    18781 cggtttttct tataaatgtc gttttcatca taccctccta ttatgcttta gtatagctca
    18841 aaatacaata aagaaaaaga atgatcctag tgtaaacaaa attaattccc tatcgtctaa
    18901 aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta gattataatc aagcttaaaa
    18961 caatcccaac acttctccgt catttgtcac gtccatattc aatgcagcag gttttttagg
    19021 caaaccaggc atcgtcataa catctccagt caaggctact aaaaatcctg cccctaattt
    19081 tgggatgatt tcgcggatcg tgatggtgaa atcttccggt gcacctagca gtgaaggatc
    19141 atcagaaaat gaatactgtg tttttgccat acaaatcggc agtttgtccc atccattctt
    19201 cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa acaacaccat ttcctccgta
    19261 aattttcttg acgattgttt ctgtcttttc ttgtatcgtt tcttctaggc ggtataatgg
    19321 cttgtaatta gcttcttttc tatcaatcaa tcgtactaca gcttttgcta aatcaacgcc
    19381 gccttctgct ccatctgccc atacactcgc tctcttggca agaatcccct gattttcaca
    19441 caagtgctcc aacagtgtga gttcattgtc tgtatctgtt acgaattcat tgattgcgac
    19501 gatcacaggt agctgatatt gttccatatt acgtatatgg cgctttaaat ttgcaaaacc
    19561 tactttcaaa gcatcaagat tctcattttt aagttcatct tttttcattc cgccatgcat
    19621 tttcaatgca cggacagtgg cgacaatcac cacagcatcc ggcgcttttt tcaagttagg
    19681 tactttgata tctaaaaatt tctctccgcc aagatctgca ccaaatcctg cctctgtgac
    19741 aacgtaatct gctaatctca atgctgtctt ggtagctaaa atactattgc agccatgagc
    19801 gatattggca aacggtcccc cgtggacaaa agctggtgtt ccgtaaattg tttgtacaag
    19861 attcggcttg attgcatctt tcagcagtaa tgccagagcg ccttctgctt tcaagtctcc
    19921 agcagtcaca ggctgacggt caaatgtata gccgacaacg atccttgcaa gacgagcctt
    19981 taaatcttca aggtctgccg caagacacaa gatagccatg atttcgctgg caactgtgat
    20041 atcaaaccca tcttctcttg gaacaccttg gattggccca cccaagccaa caattacttt
    20101 acgcagttca cgatcattga gatcgactac ccgtttccaa atcactcggc gggcatcgat
    20161 gttcaattca tttccttgat gaatatgatt atctaaaaga gcagataatg cattattagc
    20221 tgttgtgata gcatgcatgt caccagtaaa atgcaagttg atatcttcca taggcaatac
    20281 ctgtgcatat cctccgcctg cagcaccacc tttgattccc atcacaggac caagtgaggg
    20341 ttctcgcaat gcaataacag attttttatt gattttattg agcgcgtctc ctaaccctac
    20401 agtgatcgtt gactttcctt cgccagctgg tgttggattg atggaagtaa ctaagatcaa
    20461 atgcccctca gcgttatcct ttgatcgatt gattccagaa aaattgattt tcgccttata
    20521 tttcccatac agttccaaat cgtcttcctt aagatctaat ttactagcca cttcaacaat
    20581 cggttctagc tctgtttctt gtgaaatttg cagatcattc ttcatttgat tacccctttt
    20641 cacgaacttt attcaacaaa atatcttctt tgttaagata tgccaattat accttttttt
    20701 attcagaaaa aaaacataaa attacaggtt tttaatttat ttgacgtact tataaatact
    20761 ctttataatg aaggctatga tcagacttta gaaaggtcgt gagtgctttg accacaggaa
    20821 ttgtaaaatg gtttgacaac aaaaaaggat acggctttat cagttatgat gacacagaag
    20881 aaatttttgt acattttact gcgattgaag aagagggatt caaaactttg gaagaaaatc
    20941 aagtggtcga atttgagatc atcgaaggga atcgaggaac acaagctgcc catgtcaaga
    21001 aaggcaacgt tcagactacc gatactagta tgcatgaata gataaacaaa tagagtgaga
    21061 cgctaaacgg gatgaaccgt acgtctcact cttttctttt cctgtaaaaa ttcgtttttt
    21121 taggttctga tttttaaaat tagctttttc tttttttcgt ataaggataa ggaattgatt
    21181 ctgatttggg tccgtttttt tcaaaatgat caaaaaggac tacttgatct ttttttcgca
    21241 tcatgaaaac agatggggct ttctcatctt gcaacatcat ttgcacccca gtattagttg
    21301 gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc tttccaaaaa gcagcataac
    21361 gtataatcac agagtttgat tttaaaataa aagaacgccg caaacctaga aatagaaaaa
    21421 caagaaaaat acctaaaaca aaattgcttt tccaataagg acctgtgttt tctaaagaaa
    21481 gaatcagact aaaaaacaga atgatcaagg tcaatgacca atagatgatc gtatgtgcca
    21541 gttcaggctg ccatcgaata ttttttttca ttctctaacc tctcaccgat tcagtaatga
    21601 tataatctta ccataacatt agtttgaaca aaaaggagaa tctcttttta tgttaaaagt
    21661 atacattgat gcctctacaa aaggcaaccc tggtcctagt ggaggtggaa tcttgattgt
    21721 ctatcaaggt aaacaagaac agctatctat cccgctttct atagggacaa accatcaagc
    21781 agagttcgaa gtctttttaa aaactttaga atttctaaag aaaaacgact tacagaatga
    21841 aacgattttc tgtttttcag acagcaaaac actagtttct acggtcgaca aaaacgcaac
    21901 gaagaacgag ctcttccttc cctaccttca aagaatccaa gaacttctat ccgaattttc
    21961 tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa ggagcagata atcttgcacg
    22021 tcaggctctc cataaacaat taaaagaaaa aaagactctt taaattaaaa actttaagga
    22081 gtctttttat agtttctcat ttaatataaa agataattgt gatcaaacgt aatttccaat
    22141 tggtaatctc cgattttttc ttctatttga ctacgtaaga ttctttctag ttctgtatca
    22201 acaaactgga atttctcagg gaccactaca tcaaattgca gcgtttttcc ttgatgaatt
    22261 actctaaaat catgaatatt aagccctgta cctgtttcaa gaagaatttg ctttaactgc
    22321 cgcaagatat tatagtattt ttcgttatca acaggcattg gatccaagtg acagacaagt
    22381 tccacatcca aagacttttt aaagtctttt tctatcagat caataatctt atgagccaca
    22441 ttcaaattca ttgattcatc tacttcgatg tggactgatg caaagcgttt attgggtcca
    22501 tagttatgca ccaataaatc atgatagcca agtatatcat cgcaagcatt taaatgctct
    22561 tccatttctt caatctctgc tggcgttggg cgacttccta atagttcgta tacaaaattt
    22621 cgaagcatcg tcaatccact ataaaggata tataatgcta aagcaaaacc tatatatcca
    22681 tcaatacgcc atccagttac ccattcgatt gctgctgaaa gcaatacagc cagtgtagta
    22741 aagacatcat tataactatc tgttgctgta gctctcaatg tttcagaatc aatggattgc
    22801 gcaataacct gatacatttt gccttgtgca aatttcaaaa agatggaaat gattaggaca
    22861 aaaaagacga tgggtgttaa agagacattt tctggatgga tgattttttc agcagaagac
    22921 tctaaaaatt gaaatcctac ataagtaatg atgatcgaaa caaataaccc actgatatat
    22981 tcgaagcgtt cgtgtccata aggatgttcc tgatctgctg gctttgcagc aattttaaat
    23041 ccgatcaaag tcaatatgga tgaagcagta tctgatagac tgttcactgc atcagccata
    23101 atggagacac ttcctgaaaa aagcccgatc attagcttag agacaaacaa gatcagattc
    23161 gtaaccaatc ctaagatacc cgctagtact cctttttttg tccgctgttg cggtatttgt
    23221 ttcttttttt cttcttgttt cttagccaag ttttttaccc cttctagttt gcttgttgct
    23281 caggtaaaaa gatggagagg acgattgttt tcctctcctc tcttcctttt tctatctatc
    23341 gtgcgtccta ttactattaa aagatggtca ttattctggt ttcaacggtg ttgctgcaaa
    23401 taacgctttt gctagtaatg gtgttatgat tcccgcacct attgcttctg gtatcccatt
    23461 gattgctgca acccctgcta aggttttaaa taaaagagca gagtctacac cataggcatc
    23521 tgccgttgca cctgtataaa ataatcccat gaatcctaac actaagactg tattgaccaa
    23581 tgaacccagt acccctgcaa aggctgagct gatcgttatt gatttatttt tttgataaat
    23641 ccagtggaaa accaagcctg ctactaagcc aacaagtact ctgggaacta ccgagataat
    23701 cggattcgtg aacactaatg tatcaaatgg cgttgttggc atggcatagg cacgaacaat
    23761 tgtagcgatt ccccaaaaca agccaatcac catgccatct tttgggccta atactactgc
    23821 agcaataatt actgtgatat ggataattgt taaactaatg aatcctaatg gaataaaccc
    23881 cagccaagga accatcgctt gtacgatgat gattgctaac aagatcgcac gtaagactag
    23941 tcgaaaggtc ttatttctac tattcatttt ttcacccctt tttatttata ctaatcgtaa
    24001 aaatttctgt cactttcgtc aactaaagac catccgaatc cgaccatttg attagatgcg
    24061 ccatccaatt gaaaaaagta tgagggcaag acttgtttgt cttcgccccc ataatcatgt
    24121 ttcgttcaat gtttgaactt ttgtttttct gtcaaacgta aacgccctat tgttgcgcaa
    24181 cccattcttt cgcagcttta agtgcatctg gaataccagc aggatttttc ccgccagctt
    24241 gtgccatgtc tggacgtcca ccaccgccac cgcctacttt cggtgcgatt gctttaatca
    24301 agtcgccagc ttttagtcca gcttcatttg attctttcgt cattgccgct agcagactga
    24361 cttttccttc ttgttcgttt gctaaaacaa gaatatctga aatatttttt tgtttccatt
    24421 ggtcagctaa ttgacgcagc tgattcatat ctttcacttt tacttgtgct gcaatgattg
    24481 tttttccatt gatcgtttct ggatctttga atacatcacc agcttgttga ttcgctaact
    24541 tgctagctaa ttgttcattt tccttttgta gctctcttac ttgctgttgc aattgttccg
    24601 cttttgaaac aacttctttc aattgaggtg atttaacaat tgttgctact tgttttagtt
    24661 gtttttcttc ttcttcaagt agttcgtacg cttctttact tgttacagcc tcgattcgtc
    24721 gcacgcctgc accaattcct gattcagaaa cgattttgaa gattccgatg ttttctgtat
    24781 ttgctacatg tgtgcctccg cacagttcga ttgaccagcc gccaatattt actactcgaa
    24841 cgtcatgtcc gtatttttca ccaaacaaag ccattgcacc catgtttttg gctgtatcga
    24901 tatctgtttc aattgtttca actgggatag cttcccagat tttttcgttg acgattcttt
    24961 ccatttcagc taattcatct gaagtgattt gaccaaagtg ggtaaagtcg aatcgtaaat
    25021 gtccaggagc gactaaagaa ccagcttgat ttgcatgctc gccaagtact tctttcaatg
    25081 ccttgtgcag tagatgggtt gctgtatggt ttttgatgat ccgattgcgc atcgattgat
    25141 cgatcaatag atgataggtt tgtccttcac tcattggtgc ttttacttct acggtgtgca
    25201 taaattgacc attaggcgct ttttgaacat ctgtaacaac tgctacgatt tcgcctactt
    25261 cattcatgat ccatccttga tccgctactt gcccacccat ttctgcatag aaaggtgttt
    25321 gatcaaagat catttgagct tcaccttctg cgacttcatt caaaagagac tcacctttaa
    25381 cgatgaccaa taattgacta tctgattctt gttctgtgta tccaatgaat ttactttcta
    25441 ctttgatatc tgtcaataaa gcagattgta cccccattga tttttcagta ctgcgggcag
    25501 agcgagcacg ttcgcgttgt gcatccattt ctttttcaaa accagcatga tcaactttca
    25561 gtccttcatc ttctgccacc tcttccgtta attctaccgg gaagccatat gtgtcgtata
    25621 gtttgaagat gtcttttcct gctagagtcg tcccgttttc gttttttact tcttggatca
    25681 cttgatttaa gatttccaaa ccttcattga ttgtttcatt gaaacgttct tcttctgtac
    25741 ggataacttt ttcaataaat tctttttgtt gaagtacttc aggataataa cttaccatga
    25801 tttccccaac aacagggact aaacgatata agaatgcttg ttcaatacct aattttttcc
    25861 catgcatgac agcacgacga agcaaacggc gtaagacata ccctctccct tcattcgatg
    25921 gcaatgcacc gtcgccaata gcaaatgaaa gggcgcgaat atgatctgcg attactttgt
    25981 aagaaatatc tgttactggg ttttcaccat attttacttg tttgcttagc ttttctacgg
    26041 cgtggataat aggcataaac aagtctgtct caaagttcgt tgacgcatct tgaacgattg
    26101 agaccatgcg ttccaatccc atccccgtat caatgttttt atgaggaagt ggttcaaatg
    26161 tgttatcagg cttatggttg aattctgaaa atactaagtt ccagatttct aaataacgtt
    26221 cattttctcc tcctggatag ttttcaggat cgtcttctgc taaatcgtta taagcttctc
    26281 cacggtcata gaaaatttcc gtatccggac cacatgggcc tgcaccgata tcccagaagt
    26341 tgtcttctac atcaataatg tgatctagtg gcaggccaac ttcttcatgc cagatgcgtt
    26401 ttgcttctgt atcttttgga taaactgtta cataaagttt ttctggatcg aatgccatcc
    26461 attttggatc agtcaaaaat tcccatgccc agtggatggc ttcatttttg aaatagtcac
    26521 caatcgaaaa attacccagc atctcaaaca tcgtatggtg ccgtgctgtt ttaccgacat
    26581 tttcgatatc atttgttcga atagattttt gggcatttgt gattcttgga ttttctggca
    26641 caactgaacc atcaaaatat tttttcaatg ttgcaacacc agagttgatc cacaataagg
    26701 ttggatcgtt cacggggatc aaagacgcac taggttcaat cgtatgtcct ttagattgga
    26761 agaaatcaag aaacatttgt cgaacttgtg cactacttaa ttgtttcatg tcattacctg
    26821 ctttctataa attaaataca aaaaaacacc atcacagcca aggacgaaaa ctcgcggtac
    26881 caccttgatt gcaatgatgt gtaatcatta cctcttaaac tcgataacgc tgagctgcgt
    26941 cggtatttct accaaaatag aaaaagggag caatttgatt agtgactatt cttttttcag
    27001 ccaagaaaga attttctgtg agtcttaaaa aacaaatcat atccttttcg tacttaatta
    27061 gtatacgcat tctattgatc actgtcaata tctcaatttt tattttcatt tccttttggt
    27121 catactccac gatttttggt acgataagaa taaaggaatc aatacatctc tgctattcat
    27181 gacaaggagg aatatgatga aaaaaatagt tggagcggat gaatggatac gcgcagctag
    27241 ctgctttttg agatatcaag tttttgtatt agaacaaggg attcctgcag aactagaatt
    27301 cgacggaaaa gatatcccgc aacaaaccta ttatctattt cttgatcaaa aaagaccagt
    27361 agctactttg cgttatgaaa agaaaacacc tacttgcttc aagccggaac gattttgcgt
    27421 agcaaaagaa taccgaggaa aagggttagg cggtcagttg cttgaagctg ctgaaaaacg
    27481 ggcggtttca gaaggttgtc agactagtta tgtagtagct gaaatgacgg ctgtagattt
    27541 ttatgaaaaa caagggtatc atacttgcac agaacctttt attgaagatg aagtactttg
    27601 tatcggaatg aaaaaaagtc tagcttaaaa acaagagatt gtgacacgcc ttttgtcaca
    27661 acctcttgtt ttttatttat gtgaattttt cttattttta cgagcagtac ggctttgctg
    27721 tctgcgttcg attttgcgtt cttttttatt tttttctgca attgcccaat cgattttttt
    27781 cttataacca ggcttgatct ttttcttttt cttcttcaca agaccaatca gcgtgggatc
    27841 taatacatct tttgattttt ctcgtttggc acgtctatta cgatcgtatg ttttgacgat
    27901 ttccccgttt ttgatttctt ttggttcaaa tgagacgccc aatttttcta tcgcatcaat
    27961 ggcttgctca tcatttggcg aatataacgt aatggccgtt ccttttaagc cattacgtcc
    28021 agtacgtccg acacgatgga taaagaagtc tagatcttct ggaatttctg cattaataac
    28081 atgagagaca ccttcgatat cgatccctct agcagctaaa tcagtcgcta caacatattg
    28141 atactccaga ttctgtactt gtcgcatcac tcgttttctc tctcttgggg tgatgtctcc
    28201 atgaatcttt gctactttca agccttggtt tttcaagtaa tcggcaattt catccacatg
    28261 ctgttttgta ttggcaaata cgattgctaa ataaggatga ccaattgtca gcagttgata
    28321 aatcatttga ttctcatctt taccttttgt tgaaatcagc caattttcaa tgtcttcaga
    28381 aataactgct tttggtttga tttcttcaat gattggattt tccatgtatt ttcgtaagaa
    28441 tggacgaagt ttttctggaa tagtggcaga aaaaactaaa aactgcagct tttctggtaa
    28501 tcgacttgca atttggtcta cttcatttaa aaaacccata tccaatgtca tatccgcttc
    28561 atcgacaaca aatgcaaatg cggtgtgaat cttcagcgct tgttcattca tcatgtctaa
    28621 tattctacca ggcgtgccta ctacaacctg tggctgttgg tgatgcagac gatttaattg
    28681 acgctgtttg tctgtaccgc cgacaaaatt cgagacacgg atctccggtg tagaaaattt
    28741 agcgatttgt gtagctgctt catagatctg tgcagctagt tccctacttg gagctgtaat
    28801 aacaacttgt acttcctctt tttctgcatt gattttttca aacaaaggca acaaaaacgt
    28861 atgtgttttt cctgtaccag tttgagattg cccaatgata ttttttcctt ttgcaattaa
    28921 aggaatcaaa cgttcctgga cctctgtggg ctgtttgaag ccctttgcgg ttaacgcttc
    28981 attgataaac ggtttgaagc ggaatatatc aaaattattc attcgtgcac ctcttctctt
    29041 taaattatca cgactttggc attataacac agacaaagcg tttttgctca gctaatttcg
    29101 agaatcatca tcctttgttt ggccaatcgt gctaacagta tatgtgtgac tagctttttc
    29161 ataaaacaga atccgttttt ttctcaaaaa tgatagcaag atatttaaaa taaaaatacc
    29221 ttgtagtaac aagacaccga gagaaatgaa tactgcactc atctgcaaca ccgtattcgt
    29281 tgtcgaattc atgatttgct gaagcaactg ccccaaccag cttaaaccat aaaaaagaaa
    29341 gtacagatac ccgtagcgga tcatcaatgc ttttaaagaa attcgttcgc ggtcttcttc
    29401 caccaaacgg atacgcacaa tttttttgcc aagtgtccat ccattggtta taaacggtac
    29461 aatcataaag tataccccta cttctaataa aagccaccag atatgagaag taactgcccc
    29521 tgaatcgaac gcaattttaa agaaaatcgc cgctaccaaa ttaataagag ataaaaatgt
    29581 ccagtcgatc aaataagcga ccaagcgtcg agtcaatgag acagtctttc ctttttcata
    29641 agaaagttca tccattcgtc ttcttgtagg aagcataaat gtaaataacg gcgtaacgac
    29701 aaaaccaatg attccaccca atgtatttgt cactaaatca ttcacatcaa ataagcggta
    29761 tggtcttggg tagataaaat aaagtccact tagctgcgtc aattcaaaaa atagagaaag
    29821 gcagaaacta gcaatcatcg ttttcataaa agaaagccgg aaataatatc ttaaataaat
    29881 gccaaatggt atgagtaata aaacattaaa agctggttcc aaaaatacac tctgcttcat
    29941 tgccggcagg taggtactag gttcagacaa gaccaatacc gtgttgtctc tgaaatgttg
    30001 ccatgaagca aataaatgca gctccatctt tggccctgtc atttgggcaa ccgtctcctt
    30061 tgccggaagt ggtaagatca ccaaaaaata cgcacacaat aaataaaaaa cgaaagaata
    30121 caaaataact gcccttgtta ctagaaatgt tccatacttt cgatattcat aaataaaaaa
    30181 aacagatgag atcgctaagg ctaaaaatgg aaaaacaatc agcgcaaaac gaatcggttc
    30241 gatataactt gccatatgac tcctccttga ttctttgtat ctctcttatc ttagcagtcc
    30301 tttgctgttt caagcaagta acgaggctat tatttctcat ttctttcaaa gtaaagtaag
    30361 ataaacacaa agggggtgtc gagatgctga atatgtactt tgtttttggc gtacctattt
    30421 ttttactgtt cctttatgct acgatcgctt atgtaagaaa aagaactaca attcattatt
    30481 taggatttat tctcctgatt atttcgggtt tcatgctagt cttcaatctt cagacttggc
    30541 agcaagcact tcttgaaatg gataaaatga ctccacatgc tttgtctaaa gtgcttggct
    30601 accctgtcta tctgatttgg cttcccattt tcatttctgg ttgcttggtt ttattaaata
    30661 tttatcgcgg cgtacgccga atagttcagt tacgcaaatc aaagtaaaag acagaaatcg
    30721 gttgagcccg atttctgtct tttactttgc tatttcatcg tagacaaaac gacatttacg
    30781 tgatcaaaat taacgagtac ttttgacatt tcttccacgt actcctcatt taaataaaat
    30841 attttgtttg gcagagaagt gcagttctaa taaaattcga atgttttagc tatctacgtt
    30901 ttaaatagag ataaatcggg ataccagcaa gtgtcaatac tatccccatc gaagctaaaa
    30961 aggtctgagt gataagcgtg cttgatacga taaaagtccc tccaatgatt gctactagtg
    31021 gaataaacgg atacattgga actttgtatg gtcgaaacaa atcaggttct tttttacgaa
    31081 gaagaatgac accaacaaag accatcgtat aaaaaatcca aatgacaaag atcagcatat
    31141 ccgttagtgt atcaaaacca ccgatcatca tcatgccaat agcgatgact agctctaaga
    31201 tacctgcggc tacaggaact ttagttttat catgaagttt agcaaagagc ttgctaaaag
    31261 gcaggttgtt ttcttttgcc ataacataag ggagacgcat tccagtaagg gtatagccat
    31321 tgatggttcc ataaacagaa atcaagatac caactgtgac cagtcttcca ccgaagccgc
    31381 caaaaatcat ttttgcaacg tcacttgccg cattgctgtt tcctgcgact ccgtcgatag
    31441 atgcggttct taagaacacc gcatttacta ataaataaac gatcatgatt ccgatgattc
    31501 ctaaagaaat tgcttttggc aaatctttcg ccggcttttt caattctccc gaaatatttc
    31561 ctacgtgtat ccaaccgtcg tatgcgaaca tcgttgctag caagcccgct cccaaggctg
    31621 agaaaaatga aagattttct cctgcttgga tcggaaacag ttggaagtct actccttctt
    31681 gtcggaacaa accgaaaatg acgatcacaa acaaaggaat aagcttgcag acaagcgtaa
    31741 tcgactgaaa agcccctcca gctttagaac ctaaaaaatt gatcaacagg atcgatacag
    31801 cagctgtaac agctacggga acaatcattg actgggaaag accaaataga ttgacgaact
    31861 gtgtaccgaa aatgatcgac aaggctgcta catttgctgg aaaataaatc acgacttgcg
    31921 cccagcccaa taaaaaagct gctgtatttc cgtaaatccg ttcgatatat ttaatcatcc
    31981 ctcctgtttc tggaatagca gctgccagtt cagctcctgt tagtcctgca cagacagaaa
    32041 tcataccacc gagaaaccaa gaaaacatat gcaaactggc ggatcctgta acttctgcta
    32101 cgcttgcggc tttaaaaaat acccctgcac cgatcactgt gcccataaca gtcgataaag
    32161 cagtaaaaaa tcccatcgta cgttttaatt cattttctat ttttacttct tttactggcg
    32221 tggcttgata ttcttccatc ccttgtcccc tttcatacca tagccaatcc caaaagtata
    32281 aaaaactttt tggatgtggt tcttgtttta taccgtctct accaattttt ctttcagttt
    32341 ttccaacata tcctttgtca tcacatctaa gtcgtatttc ggcgaaaagc cccattcctc
    32401 ttgcgcacaa gaaacatcta gtgaatttgg ccaagattct gctattgact gacgtacagg
    32461 atccacatca taatccattt caaaatctgg tatgattttt tgaatagatg ctttgatctc
    32521 ttccgggtca aaagacatcg ccgatatatt gaaggcattt cgatgaacca atcgatcagg
    32581 atctgcattc atgagtttta taatggcatc gatcgcatct ggcatataca tcatatccat
    32641 tgatgtacct tgatcaatga aactagtgta ctttccttct ttgattgctg aataataaat
    32701 atcgactgcg tagtctgtcg taccgcctcc tggcaaggtt gcataagaaa tcaatccagg
    32761 gaaacggaca ccacgagtat ctacgccata tttcacataa taataatcac atagtagttc
    32821 tccagctacc ttcgttaccc catacatggt tgtaggacgc tgaagagtat cttgtggggt
    32881 atgatctttt ggagtatttg gaccaaacgc gccgattgaa cttggcgtaa aaaatttcag
    32941 ctggaattct ctagcgactt ccaatgcatt gaccagccca gtcacgttca aattccaggc
    33001 taattgtggt tttgcttctg caacagctga caaaagagca gccaaatgaa tcaatgtgtc
    33061 cacttgatat tgttctgcta tgctgcgcat tttttcgtaa tccattacat ctaggatctc
    33121 aaagatatcg tttttctcaa tgacttcagg catgcgtata tctgtcgcaa tgacattttc
    33181 actgcctagt tcttgacgta gccgtgtgat caactctgaa ccaatttgtc ctaaacatcc
    33241 tgtcaccaat acttttttca tgatgatcct ccctgatctt ttttactact atcttcagat
    33301 gattaacaat atatgcgtga acatgttctc taattttaaa tagacgaaaa aactaaattt
    33361 agttaaataa tttccatttc ttttcctatt ttttgataaa ctgctactgc ttcatcaagc
    33421 atttccctcg tatgttccgc agtcggcata tttctgatcc tgcctgtccc aagaggcaca
    33481 gttggataaa cgattggttt agcataaatc ccatgttcga tcagcttctt agagaaagtc
    33541 tgagtcactt tttcgtctcc caaaatgact ggagtaatag gtgtttcaga cataccgatg
    33601 ttatagccta cttttttcaa ttcttcttta aaataattag cattttccca aactttttcg
    33661 acaagttccg gatgttcttg catcaaagta atcgaagcca atgcagctgc agctgcacct
    33721 ggagttagcg aagtagaaaa taggaatggc cgacttctag ctttcaacca gtcgattagt
    33781 gttttcgatc cagctacata acccccaaca acacctatag ctttcgataa cgtgcccatt
    33841 tgcatatcga ttttgtcgct taatccaaaa tgcttgacgg ttcctgcacc ttttccagtt
    33901 acgcctgagc cgtgcgcatc atcgacgtag gtgatcagtc catatttttc aacaatcgga
    33961 ataatttccg gcaatcgtgc aatgtctcca tccatggaga aaacaccatc tgtgatatac
    34021 atgatttttt tatatttctt actttctatc gcttcctttg cttttttctc aaggtctttc
    34081 atatcttgat gtttaattcg gatgattttt gctccggata agcggcaacc gtcaataata
    34141 gatgcatggt ttaattcatc cgataagata gcatcttctt ttgtcattac tgcagagata
    34201 gcgcccatat tgcagttgaa tccagattga aaggcgattg ctgcttctgt ccctttgaat
    34261 tctgcgattt tttcttctaa ttgctgatgg attttcagcg aaccattgat cgttcggact
    34321 gctcctgctc ctacaccata agtttcggtt gcttcaatac atgctttttt caattcttcg
    34381 cgattggcaa atccaagata attgttggat gctagattga tcatcttttt tccgtctacg
    34441 atgatacaag caccattttc actttctagt atgtcgatcg tattgtataa ccccttttct
    34501 ttcagttgat ccaattcttt ctctaaatac attgttagtt ctttggaaga cattgtctcc
    34561 acctccatat tcatctgtct tgcagaacat accaaacata tatcgtgtca ttcatctaca
    34621 aagcaagtat gctatatttt tgataacgct tacactgatt atgagaatta tttaaccaca
    34681 ttttttattt acagtatctt tt
//
LOCUS       DACDMM010000011        33267 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDMM010000011 DACDMM010000000
VERSION     DACDMM010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.011"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            165..1067
                     /locus_tag="IWD16_000413"
     CDS             165..1067
                     /locus_tag="IWD16_000413"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP9087499.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            1280..3055
                     /locus_tag="IWD16_000414"
     CDS             1280..3055
                     /locus_tag="IWD16_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP9087500.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(3121..4707)
                     /locus_tag="IWD16_000415"
     CDS             complement(3121..4707)
                     /locus_tag="IWD16_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP9087501.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            4958..5182
                     /locus_tag="IWD16_000416"
     CDS             4958..5182
                     /locus_tag="IWD16_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP9087502.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            5324..7075
                     /locus_tag="IWD16_000417"
     CDS             5324..7075
                     /locus_tag="IWD16_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9087503.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            7075..8859
                     /locus_tag="IWD16_000418"
     CDS             7075..8859
                     /locus_tag="IWD16_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9087504.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            9185..10036
                     /locus_tag="IWD16_000419"
     CDS             9185..10036
                     /locus_tag="IWD16_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9087505.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            10090..11841
                     /locus_tag="IWD16_000420"
     CDS             10090..11841
                     /locus_tag="IWD16_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9087506.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            11845..12015
                     /locus_tag="IWD16_000421"
     CDS             11845..12015
                     /locus_tag="IWD16_000421"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087507.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(12055..12957)
                     /locus_tag="IWD16_000422"
     CDS             complement(12055..12957)
                     /locus_tag="IWD16_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9087508.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            13188..14591
                     /locus_tag="IWD16_000423"
     CDS             13188..14591
                     /locus_tag="IWD16_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9087509.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            14591..16024
                     /gene="ascB"
                     /locus_tag="IWD16_000424"
     CDS             14591..16024
                     /gene="ascB"
                     /locus_tag="IWD16_000424"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9087510.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            16169..17095
                     /gene="trxB"
                     /locus_tag="IWD16_000425"
     CDS             16169..17095
                     /gene="trxB"
                     /locus_tag="IWD16_000425"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP9087511.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            17309..19036
                     /locus_tag="IWD16_000426"
     CDS             17309..19036
                     /locus_tag="IWD16_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9087512.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            19108..19401
                     /locus_tag="IWD16_000427"
     CDS             19108..19401
                     /locus_tag="IWD16_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9087513.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(19493..20353)
                     /locus_tag="IWD16_000428"
     CDS             complement(19493..20353)
                     /locus_tag="IWD16_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP9087514.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            20445..20873
                     /locus_tag="IWD16_000429"
     CDS             20445..20873
                     /locus_tag="IWD16_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9087515.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            21035..21211
                     /locus_tag="IWD16_000430"
     CDS             21035..21211
                     /locus_tag="IWD16_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP9087516.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            21238..21684
                     /locus_tag="IWD16_000431"
     CDS             21238..21684
                     /locus_tag="IWD16_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP9087517.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(21838..23217)
                     /gene="brnQ"
                     /locus_tag="IWD16_000432"
     CDS             complement(21838..23217)
                     /gene="brnQ"
                     /locus_tag="IWD16_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9087518.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            23923..24894
                     /locus_tag="IWD16_000433"
     CDS             23923..24894
                     /locus_tag="IWD16_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP9087519.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            24918..27110
                     /locus_tag="IWD16_000434"
     CDS             24918..27110
                     /locus_tag="IWD16_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP9087520.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            27127..27603
                     /gene="ybeY"
                     /locus_tag="IWD16_000435"
     CDS             27127..27603
                     /gene="ybeY"
                     /locus_tag="IWD16_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP9087521.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            27581..27982
                     /locus_tag="IWD16_000436"
     CDS             27581..27982
                     /locus_tag="IWD16_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP9087522.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            27997..28896
                     /locus_tag="IWD16_000437"
     CDS             27997..28896
                     /locus_tag="IWD16_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP9087523.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            29039..29851
                     /gene="recO"
                     /locus_tag="IWD16_000438"
     CDS             29039..29851
                     /gene="recO"
                     /locus_tag="IWD16_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP9087524.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            30235..31152
                     /gene="glyQ"
                     /locus_tag="IWD16_000439"
     CDS             30235..31152
                     /gene="glyQ"
                     /locus_tag="IWD16_000439"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP9087525.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            31154..33229
                     /locus_tag="IWD16_000440"
     CDS             31154..33229
                     /locus_tag="IWD16_000440"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP9087526.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        10952 a         5899 c         7091 g         9325 t
ORIGIN      
        1 gaaaatgatc tttattataa aagagttctt aagcttttct ttagagaaaa tttcagttac
       61 tatggagaac agggcaaaga aaaatcaatc attttctttg tatatcatct taccaatata
      121 taggaattgt tagtaacaat tgcctttcaa ggaggaatcc atccatgtta ttaggctcac
      181 acgtcagcat gagcggaaag aaaatgctgt taggagcagc agaagaagca gccagttatg
      241 gatcttcgac ttttatgatc tatacaggcg cccctcaaaa cactagaaga aaagccatcg
      301 aagagatgaa tattgatgcc ggaaaagcgt acatggcaga aaaaggtcta acaaatatcg
      361 tcgttcatgc cccttatatc attaatttgg ggaatacgat caaaccagaa atgtttcctt
      421 ttgccgtcca atttctgaga gaagagattg ttcgtgcgga agctttaggc gctactcaaa
      481 ttacacttca cccaggtgct catgtagggg caggaacaga agccggaatt gctagaatta
      541 ttgaaggctt gaatgaagtt ttgacaaaag aacagacggc acaaatcgct ttggagacaa
      601 tggcaggtaa aggtacggaa atcggtcgaa catttgagga attagcgcaa atcatcgatg
      661 gagtaaaatt gaatgaaaaa ttatccgtta cttttgacac ctgtcatacg aatgatgccg
      721 gttataatat taaagaagac ttttcaggtg taatggaaga gtttgataag atcatcgggt
      781 tagatcggct taaagtactc catatcaatg attctaaaaa tccgcaaggt tcgcataaag
      841 accggcacgc caatattggt tttggaacaa tcggattcga cacgctgaat aagatcgtcc
      901 atgatccaat gtttgacaat atttcgaaaa tcttggaaac tccctatgta ggcaaagata
      961 aaaagaaagc ttacgcacca tatggaaaag aaatcgccat gttcaaagcg caaacattcg
     1021 atccagaagt gtttcctgag ttattagcag aagaagtgat actctaagat tttctatctg
     1081 acagcagcaa aaaaggacca ggcacattgc cttgtccttt tttgtttaag aaacaaagga
     1141 gtactttttg cagtcttttt catcatgcat cttctccccc ctctttttaa atttagacaa
     1201 acaaggtata ctggtagaaa agtaaaaaaa cgaaaagttt cacaagcaag tggcaaaacg
     1261 tgattggaag gacgaaaaga tgctgaacac tttaaaaccc ttgatcaacc aactaaaaaa
     1321 gaaacataca tactttattc ttggcatagc cctattattt acaattattc gtttgtattt
     1381 atatagtaaa gcaatctatg gaacagacat gtcggcaacc tatgatgacc agctgctgat
     1441 tcgctatgct caaaatattt tggacggtaa atggctaggg gagtatacga ctacaacact
     1501 ttccaaagga atctcctaca gcttattttt agtacttgca cataaactgc atctttctta
     1561 tgcagttttg ttcggcatgt tcagtatttt tgccagtata ttggttgcac tcgcgtttaa
     1621 accgattgtg aagaacaagt atcttctttt aagtatctat ttttattttt tgttttctcc
     1681 gattagtttt acacatgaat attccactcg aacatatcgt aatgccctcg tgattccggc
     1741 tgttgagatc attatcgctt gtttattagc tatttattac cgcaaatacg gttctagcag
     1801 aaaattgctg ccatggtttt tagggctttc tgtcgctttt cctttctttt ggttcattcg
     1861 cgaagattct atctggcttc ttccttttac tgtagtagct ttgggtatca gtttcgtcca
     1921 gatcttgcta gggcaagact tttctttcaa agaagtaaag aagttaaaaa aacgactctt
     1981 tcttctctcg aaacgggaat ggctgaaact ttcattgttt ttacttccta tagttggctt
     2041 ttccatcgtc tatcaatgga tcgaatataa aaatgagaca acatacggta ttgcaaccat
     2101 caatgaccgt tccagtggag aatttggacg tttgatgaaa caactgatcc gaatagatga
     2161 tgaaacgaac ttgaataaag aaaacagtga gatatgggtc tcacatgctg cactaaataa
     2221 agcattagcc gcatctccta cattccatac tgtttctacc cgtattgacg aattatatac
     2281 gactcacgcg tggactcaag gtggaaaagt aaaagaatta cccggagata tcatcttttg
     2341 ggcattgcga gatgtggtaa gcgaatctgg ttattaccaa aacaatgcaa aagaaaccaa
     2401 tgatttttgg aaaaaagtca accaagaatt agaagaagcc tatcacaagg gaacaatcaa
     2461 aaagaaaaaa gagttctatc tgatgggatc aggagatgga aagacgatga gtgatctgcc
     2521 aatcgtattc gatttccttc aatcaggata cgcttatgca tggcgatata aagatttcca
     2581 tcaaggagga aattatagtt tcggaacgca agaacaagtc aaacaggctc aagaattatt
     2641 aaaccttccg ctaatggaga attggatcga tagtaatgaa ccgagaacac ctaatttcca
     2701 attaacaaaa tcagcaaaag tcgcgaatgt catcataaaa atttaccaag ttctggctat
     2761 tcccgtgctg attgcttcaa tagcagggat attacttatg atactaggaa ccgtatttgc
     2821 aaaagctgat cgtgcctatt atagttcctc tttgattgtg gtcaccggat taatactgac
     2881 acaattggtt tttcttttcg gtgtctcatg gttttgttct tatgcgccag aaaaaaagga
     2941 ctatttttta agtgtctaca ctggtgcagg cgtgccatta acgcaattca tcgtagtaac
     3001 aagcatgcta gttttcagta aattaaatct gaaaaaagct ttatcttcta aataaaaaga
     3061 cccttggtag ctagattttt aacctaacta tcaagggtct ttgtgtggaa aaatcagttt
     3121 ttattcgatt tcttctaccg tttcctgcaa aagctgttca tattttttgt ttcctttata
     3181 taaatcacca atcgtccatg ccaacaggag aaggacaaga acaatgatta cataagcgat
     3241 gtgatcagcc aatgctagtt gagcggaagt ggcttgctcg ccgaagaaac tttcgatctg
     3301 tcctggtaat cctcgtaagt tcaagtaagt tagactaatc acagagaacc agccaagaag
     3361 ttttacgatg aatgaatttt taaatcggct acccatctcc gcttggctgt tggtcatcat
     3421 cagtaaagga atcatcgaaa acggcaacgc aaaagccaag aacacctggg attcattcat
     3481 taagttattc aaagcggcat gttcttcgat cgttcctttt gtacttgtaa acaaaacaca
     3541 aatcaataca ggaatcacag aaagcaatcg tgtgaccagt cttcttagcc aaatcgggat
     3601 acgcatatgg atgaatcctt ccatgatgac ttgaccggtc aacgtaccag taatagtaga
     3661 gttctggcca gaagcaagaa gtgctacagc aaataaagtg gacaagatac cagattttgc
     3721 tacagcaatc aaaataccat tgctcatagc tgatggatca gataaagctt catacagacc
     3781 aaaaaaggaa ggatcttgga cagaaccggt tttgaagaca gcgacaccca tgatcaatag
     3841 cagtgcattg actagaaaag ccattgacaa ctggatatta gaatcccaag tagaaaaacg
     3901 tacagcttgt gctacatctt cttcgttttg gtgatcgatc ttccgagttt gtgaaactgc
     3961 tgaatgaaga tataagttat gcggcatgac tgttgcacca ataatcccta aagctccagt
     4021 cagcggagtt tcgccgccga tgaccggtga gtctgagaat gtatgactgt tagggataaa
     4081 gccagcaaat aaactgtgcc aatctgggtt agacaagcca acttgataga caaaaacgaa
     4141 taaaatgacg aaaattaagc agacaaccag tgcttcgatc ttacgaaacc cgacttttgt
     4201 cagtagtaag agaacgaaaa catctaatac agtaataaag acggcaatca ccaatggaat
     4261 atcaaaaagt aagtagaggg cgattgctgc accaatgact tctgcaatat ctgttgccat
     4321 aatggctaat tcagtcaaga tccataaaat gacacctaat gtcttacttg tgcgtgctcg
     4381 gattgcctgt gccaaatcca tttggctcac gattcctaat tttgctgcca tatactgtaa
     4441 aagcatagca atcagactag acatcaaaat aactgacatc aataaatatt ggaaattttg
     4501 tcctccggtg atcgaagtag accagttacc cgggtccata tacccaactg ctactaacgc
     4561 accaggtcca gagtaagcaa gaagtgtttt aaaaaaactt ttcccctttg gtacatcaac
     4621 tgttccattt acttcagcta aagataaacc atttgtatgt tcaataagtc gatgcttatg
     4681 aaattgttta ttggattgat tattcaagaa aaaaccacct ttcctattta tctacgatta
     4741 cattctacac ctcaaaaaga aaaaattaaa gaaaaagatt cggtacgcct aatttttttt
     4801 catggcgcgt cttgctagat gacgaaagaa attttgttct cctctaaaaa aaccactatt
     4861 ttttgcatga aactaaagaa tacgcctttc ttttttcaat aaactttagt aaaataagtc
     4921 attgagagca attcggaaat agaaaggaga gatttatatg ttatcaacag gtattgtcgt
     4981 acttattgct attattgctt tgctaatcgg cgcagtaggt ggctttttcc tagcgcgtaa
     5041 atatatgaaa gactatttag aaaaaaatcc tcccgttaat gaggacatgt tgcgatcaat
     5101 gatgatgtca atggggcaaa aaccatctga gaagaaaatt cgccagatga tgcagcaaat
     5161 gaagaaccaa gggaaaaaat aggttctgac ccaacgactt tttacgggct gattttttca
     5221 gtccgttttt tatttcgtaa tttgttagac tattcagatt attcagcaaa aaagacaagc
     5281 acagacaatc ctaggctctt gataaagaca aaggaggagt aaaatgtcga tttttagaaa
     5341 attaggctgg ttttttcgtc aggaaaagaa acattattta attggggttt tctcgctatt
     5401 cgcagtggcc ttggtacagt tagttcctcc aaaagtgatt gggatcatca ttgatgagat
     5461 cgccgataaa aatttatcaa taaagatcat tttcgggtgg atcttcctat tgattatcgc
     5521 tgctttttta caatatcttt ttcgttatat ctggcggacg aatatttggg gaagtgcagc
     5581 tcgattggag aaagaacttc ggcagcagtt attcaaacat tttacagaaa tggacaatct
     5641 cttttaccaa aaatatcgga ctggggattt gatggctcac gcaacgaatg atttgaatgc
     5701 gatccagaat gtagcaggag ctggtatctt gacctttgca gattcagtga ttacgggcgg
     5761 aacaacgatc attgcgatga ttttgtttat tgattggcgc ttaacactga ttgcgctgat
     5821 tcctctgcct ttgctggccg tcacttcacg agtgctgggg tcaaaactcc atgatgcttt
     5881 tcgtgattcg caagaagctt tttctagtat caatgataag acacaagaaa gtatcactgg
     5941 gatcaaagtc attaaaacat ttggacaaga aaaagaagat ctagcggatt ttgaagaaaa
     6001 gattgaagat gcaattatta aaaacaaacg agtcaatttt ttagactcct tatttgatcc
     6061 attcattacc ttgattatcg gtctgtctta tgttgcaaca attattttag gtggaaagta
     6121 tgtcttggaa ggcacgatct cgatcgggca gatggtctca tttattagtt atatcggtat
     6181 gcttgtttgg ccgatgtttg cgattggtcg attgttcaac gtattggaaa gaggaaatgc
     6241 ttcttatgat cgcgtcaatg aacttttgca tgaaaagacg catatcgtgg aacaactggg
     6301 tgcagtgcag accccggcaa aaggcgagct gaatgtagct atttcctctt ttaattatcc
     6361 gaaagaagaa tcaccagcat tagaacaaat caagtttgcg atccaagaag gagaaacatt
     6421 aggtattgtt gggaaaactg gtgcgggaaa aacgacgatc atcaaattac tgatgcgtga
     6481 atacgatgat tatcaaggga aaatccaatt tggcggacgt tcgatcaaag aatattcatt
     6541 gaatgcttta ttaggatcaa tcggctatgt gcctcaggat cacttcttgt tttcgatgac
     6601 cattcgagat aatatccgtt tttctaatcc aaaactttca gaagaagaag tagagcatgc
     6661 ggcttcttta gcatttatca acaatgaaat caaggaatta ccaaaaggtt atgatacctt
     6721 agttggagaa cgtggagtct ctttatctgg cggacagaaa caaaggattt cgattgctcg
     6781 agcattgatc aaaaatcctg aattattaat attagatgat gcactctctg cagtagatgc
     6841 caaaacagaa gaagctatct tgcataattt aaaagaaata agaaaagaga aaacaactat
     6901 cattgcagct caccgtttaa gtagcgtgat gcatgcaaaa gaaatcattg ttgtcgatca
     6961 agggaaaatc attgaacgag gaacccatca tgagctgctt gcactaggcg gctggtatga
     7021 acaaatgtgg gagaaacagc aattagaagc gaagatagaa ggaggcgagg cctgatggat
     7081 gaaaattatc aatcggaatg gacaaaatcg attccactta aagaacagaa aaagatcatc
     7141 aaacgactga tgaagttcgc caaacctttt agaagaacgt tcatcgttgc tatcttattt
     7201 gcgtttgctt tgtctgtgat caatgtccta ttgccaagga tcatccaaac ttttatggat
     7261 gatcatctgg ctaaacagtc ggctacgaca caagttattt tgttttttgc tggtatgtac
     7321 ttatttggag tcatagtaaa aagtatcatt tggtttttcc aatggtattt gtattccatg
     7381 gcctcattga agacctatca gtatattagg gtaaaattat ttgaaaagct gcatacacta
     7441 ggtatgcgtt attttgatca gacaccggca ggatcaaccg tttctcgagt gacgaatgac
     7501 acagaaacgc tatttgaatt ttggtacgtc tttttgatgg tattgactgg gatctttgca
     7561 gtcgtttctt cattccttgc catgtttcaa atcaatggag aaattgcctt gtggactttg
     7621 gtgtttttgc cgattttagg aatcgtcatc tggtattacc agaaattcag ttctcgtatt
     7681 taccgaaata tgcgggaaaa attaagtcag ctgaacacga aattgaatga atcgatttcc
     7741 ggcatgcgta ttatccagca gtttcgccaa gaagctcgtt tagctaaaga atttgaacaa
     7801 gtgaacaatg agtatttaga cacgcgttat gcgatgatca aaacgaattc gattctgtta
     7861 agtccgatca tcaattttct atatgcattg gcgattgcgt tggtgttgac tttgtttggg
     7921 atcgatgcgt tgcattctcc cgtagaagtt ggactgatct atgcatttac gacctatgtc
     7981 cagggatttt ttaatccaat gtctcaaatg atggactttt tgagcatctt cacagatggg
     8041 atggttgctg gtagtcgtat cttgaagatt atggatacgg aagaatatgc accacaacaa
     8101 aaccctggag cagatcaaat gattacttca ggtaagattg agtttcgcaa tgtaagcttt
     8161 tcttatgatg gagagcacaa tgtattaaac aatatctctt ttgttgctaa tccgggagag
     8221 accgttgcat tagtgggaca caccggaagc ggaaaaagct cgatcatcaa cgtattgatg
     8281 cggttttacg aattttatga aggtcagata ttgattgatg atcatgacat tcgagaatat
     8341 ccaatggaag agttacgtaa gaaaatgggg ctggtcttgc aagatgcttt cttgttttat
     8401 ggggatatcg caggaaatat tcggatgatg aatccagcta tcacagacga acagatacgc
     8461 catgcggctg agtttgttca agcaaacaaa ttcatcgatg aattaccaaa aggatatcac
     8521 tcaaaagtga tcgaacatgg tgccagctat tctagtggac aacgacagct gatcactttt
     8581 gcgcgaacaa tcgtgactga tcctaaaatt ctagtattgg atgaagcaac agcaaatatc
     8641 gatactgaaa cagaaggatt gatccaagaa ggattggcaa aaatgcgtca aggacggaca
     8701 acgattgcga ttgcccatcg gctgtcaacg attcgagatg cagaattgat tttagtttta
     8761 gacaaaggtc atatagtaga acgcgggaat catgaaacac ttctagcgca aaatggtttg
     8821 tatgcagata tgtacaaact gcaaagcagt ggtatataaa agatgttgtg acagtagaat
     8881 tttgacctaa gcactagagt gaaaataaaa catagcttct catatttttc atctgtgaga
     8941 gttagtgtcg tagtcaccag atttaaaaaa agattcgaca gctagtcaga agtgcctaat
     9001 agccactaag aaagcgttga caaaagcatt tgctttcgat aatataaaga aaacgataca
     9061 acttggatag tgattgcctg atgcaagcta aacctgatga gtctcccatg gtttcatgga
     9121 cggctttttg ggtttttctt tttgttttgg atacagccct atacatcatg gaaaggagca
     9181 aaacatgctg attgagaaag tactgaataa caacgtcgta ataaccaggg atgaaagaaa
     9241 tcaggaaatg atcgtgatgg gaaaaggttt ggcattcaaa aagaaagtgg gtgacccgat
     9301 ttttgactca tgcatcgaca aaaaatttca gttgacaaat gacgggcttt cacaacagtt
     9361 ccaagaattg gcacaagcga ttcctcttga gtacttagaa atttcttgtg aaatcattga
     9421 atatacaaaa gaggctctac aagtccagtt gaatgattct gtgtatattg cattgacgga
     9481 tcatttgtat acggctatcg aacgtcacaa aatgggaata tttgtaccaa atgtattgct
     9541 gtatgatatc aaacaactat ttcctaaaga gtatgccatc gggaaaaaaa cagtgaagaa
     9601 aatctcggat aaataccaca tcaagttatc agaagatgaa gccggattta tcgcattgca
     9661 tttagtcaat gcacaaggca ttcaaagcca gcaagacaat acctacctgt ttactataac
     9721 aatacaggat attctaaata tcgtccgcta ttactataag attacatttg atgaagattc
     9781 agtccatttt tttcgattcg tcacacactt acgttttttt ctgacacgcg tcctagatgg
     9841 agaaaaatca gaagtgggag aagtttctga ggaattgttt caggttattc aaaaaaaata
     9901 taaggatacg tttggttgcg ttgagaaaat agcagacttt ttagccgaga cgttcaacta
     9961 ccagatgtcc acggacgaaa aaatgtattt aacgatccat actgcgcgtt tagtgcatga
    10021 gaatcgttgt aaataaaaaa aggaggataa attaacaaga acgtatgaaa taagggtagt
    10081 gacattcagt tggctaaacc ttccactaat aaattggagg gaatagaaat gggaaaatac
    10141 caagaattag cgagaaaaat cgtagaaaat gtcggcggga aggaaaatat caatggtttg
    10201 acacattgta tcacaaggct tcgtttcaaa ttaaaaaacg aagaaaaagc aaatgatgag
    10261 atcttgaaaa atatggacgg tattgtaacg gtaatgcgag cgggagggca gtatcaagtt
    10321 gtcattggga atcatgtgcc agtcgtgttt gaagaggtta taaaagcagg aaaccttact
    10381 tttgatgagg ctgtttcaac agaaaaaatg agaccgtttg atatgcttat tgatggaata
    10441 tcgggctgtt tccagccatt ccttgctatc ctagctgctg gcgggatgat tcgtggactg
    10501 acagcattcc ttgtcttctt aggcgcgttt gaccgcggtt ctggcacatt tgttatgttt
    10561 gataatatcg gtgattctgt attccaattt atgcctgtta ttatcggttt aacagctgcc
    10621 cgtaaattta aagtaaatga attcgtaggg atgttgcttg gcgcagcatt aatgaatcct
    10681 agcttgtcgc tggaagcttt gagtggtgca gcagaagcac cactaacgac catattttct
    10741 ggcactattt ttgaagcacc tatttatcaa acagtattcg gcattccctg gattgcgcgt
    10801 aactacgctt cttctgtcat tcccattatc tttattgtgt tgttagctag ccaaattcag
    10861 aaaccaatta aaaagttagt acctgagatg atcgcgaatt tcttcgttcc attcttcacc
    10921 gttcttatta caatgccttt aggtttttta ctcgttggac cagtattcac attcgcaact
    10981 gatattttaa tggctggatt tgaaacactt ttagcacttt cgccagttat ttatggagca
    11041 atcgtaggct tcttctggca aattctggtg atgtttggtc tccattgggc aatcgtacca
    11101 atgggattga tgcaattttc tgtgaatggt tggcaaaata ttatgacacc tgttgcagta
    11161 gtgtcttttg gacaaactgc agcacttacc gcactctact ttaagcttag aaatccaaaa
    11221 gacaaagcaa ttgcaatacc agccattgtt tctggtattg tggggattac agagccagct
    11281 atttatgggt ttacattacc acgtaaaaaa atatttattt ttacctgtgt cggtggtgcg
    11341 ttagggggcg cgtatagtgg gttaatgaat ttaacttctt ggaatcaagg tggactaggc
    11401 atctttacaa ttcccaacta tattagacca gatggtgatt taacagatgt gattaatgta
    11461 ttgattggga tcgccattgc gatggtggta tcctttacgc tgactttttt cttttggaag
    11521 gatgaagcag gtgaaacaga tgatatacag aaaaaatcag gtaaagaaat tgtaaaaaca
    11581 ccgatacaag gacaaatcgc tccactaaat gcagcaaagg atgcagcttt tgcacaagga
    11641 acactaggtc gaggaatctt gatctaccct gaaaaaggag aagtacgtgc cccttttgac
    11701 ggaacagtca tgaccctgtt cccgacgaaa caggcaattg gaatggtttc tgaaacaggg
    11761 ctggaattgc tgattcatgt cggattggat actgtgcagt tggaagggaa atattttgaa
    11821 agcttagtgc agcaaggata aagagtgaaa caaggcgatc tgttgctacg cttcgacaaa
    11881 aagaaaatcg aaaaggaagg atacagttta gaaacacctg tgatcgtcac caattacacg
    11941 gattatttgg atattttgga aaaccaaggg gagacggtcg ggccagacga tgcacttatc
    12001 accgcactga cctgaacggt taggtgaaga atggactagg cttaactatt ctttttattc
    12061 cacttcttta ttttcaaaat tgaaaggctc tttcatgatg gctgagctga tagggcgttt
    12121 atcgaatttt tgaccgacag aaattagatg gactaaattc tgttgataat tttctgcaaa
    12181 aacacaacta tataacacgt ctagaagata acaaattgat gtattgatcg taaaattacc
    12241 aatttttgaa tacagtcgct cgcgtgtcgt aagtcgcaaa gcggctgtac tcattgcaga
    12301 tagcgttgaa tcaccgatac tggtcaaagc taaaattgga acatgatttt gtttcaatat
    12361 ttctgcggta cgaaggatca cattgttttc acccgtataa gaaatcagaa tcccgcacat
    12421 atcactcgtg caattgtacg cttcatagaa tgattcacca aaagtggtga caatcgtgac
    12481 atccattttg atgcgcttca ttttcagcat gaaatcttga gaaatcaaag tattcgagtt
    12541 actggcgaaa atcacgattt tttttgcttg aagcattagt tgtttggctt tttgcagttg
    12601 gtcatgatga atgagagaaa gtgtatcatg aatcgtcatc tgttctaaag aagccatttt
    12661 atttgcgatc gtcataatac cgtctgtggt ggaaaaagga agattcgcat ctacgtcttg
    12721 aaaattccct tcaagatagg ctgtttcagc taaaaaagca ttacgaaaac tgacccagcc
    12781 atcaaaaccc attttttttg ctacacggat gagtgtagag gggtggacat aacatcgctc
    12841 agcaatcgct tggatggtca tttcgccaag tgctggagaa tcgcttaaga gaaactggat
    12901 cacttcattt tctgaaggag aaaaggagat ttctttgatt ttttcacgta ataacatgta
    12961 gataccttct tcttattttt ttacgtaaat tataactttt ttgaaaatga tttgtaaacg
    13021 gatttatact atataactta aaatagacat tttttgacaa gatatttaca aaaatccact
    13081 agaaaacagt aaattttgta aatgttactg gaaagaaaca gatttctatt gtttgttttg
    13141 tttacaaaaa agataatgaa agtgtaaaaa agtaaaggag tgagaacatg gcaaaaggaa
    13201 tcaaaatcgc tacgattggt ggaggcagca gttacacccc cgagttgatg gaaggattta
    13261 tcaaaagata tgaggaatta ccgatacgag aaatctggct cgtagatgta gaagaaggaa
    13321 aagaaaagct tgagatcgtt agtcagatgt ctcagcgtat gtgggatgct tctccgtatg
    13381 aagtcaagat ctatgcaact ctggatcggg aagaagcatt gaaagatgcg gattttgtta
    13441 cgactcagtt tcgtgtcggt caattagatg cacgaatcaa agacgagcgg atccctagtt
    13501 attatgggat gattgggcaa gaaacaaatg gtgctggcgg gatattcaaa gcttttcgta
    13561 ccgttccagt cgttttggca atcgttgagg acatgaagcg tttatgtcca aatgcttggc
    13621 tgatcaattt cgcgaaccca agtgggatgg tgacagaagc agttatcaga tatggcaaat
    13681 gggataaagt gattggatta tgcaatgtgc cagtgatggc gatgatgaca gaaccagcaa
    13741 tgcttggcaa gaaaaaagaa gagctgatct acaagtttgc cggtctaaat catttccatt
    13801 ggcacaaagt agcagataat aaaggaaatg accgcacggc agaattgatt gatcgtttat
    13861 acgatgaaga gaatggatta ccgaaaaata tctttgatat cccgtttttc aaagaacaat
    13921 taaaacaaat gaacatgatc ccttgtggct accaccgcta ttactatcgt gaagaagaaa
    13981 tgctggaaca tgctttagaa gaatatcaaa cgattggtac aagagcgcag caagtgaaag
    14041 aaacagaagc agagctattt gaattataca aagatccaga attagaccat aaacctgaac
    14101 aattgcaaaa acgaggtggc gcttattatt cagacgctgc ctgtgaaacg attgcttcaa
    14161 tctatggaaa taaagaaaca cagatcgtgg tatctacaaa gaatgatgga gctgttcctg
    14221 acctgccggc agattgtgta gtagaagtca ctgcctatgt tggcgcacaa ggagcgagaa
    14281 atgtagcgtt tggttctctt cctgctgctg aaagaggctg gttgcaagta atgaagaata
    14341 tggaattgct gacgatcgaa gcagctgtga ctggtgatta tgggacagct ttacaggcgt
    14401 ttacgatcaa tccacttata cgatcaggaa agacagcaaa acggatcatg gatgagttgt
    14461 ttattgcaca ccagcagtat ttaccgcaat tcaaggagac aatcagccga ttaaaagaag
    14521 aaggaatcga agttcaagat gaactggcaa gagaattatc aggtaataca catataatgg
    14581 aggaagtata atgggattca gaaaagattt tttatggggc ggtgctacag cagccaatca
    14641 atgtgagggc ggttatgatg aaggtggacg cggactagca aacgtagatg ttgtgccaat
    14701 cggaaaagac cgttttcctg ttatcaccgg aaagaaaaaa atgtttgaat ttgatgagga
    14761 tcatttttat ccagcaaaaa ttgcaatcga tatgtatcat cgttataaag aagatattgc
    14821 tttgtttgca gaaatggggt tcaagactta tcgaatgtcc atcgcctgga gtcgtatttt
    14881 tccaatgggg gatgagaaag agccgaatga agctggcttg aaattttatg aggatgcctt
    14941 taaagaatgc caaaagcacg gaattgagcc acttgtgacg attacgcatt ttgattgtcc
    15001 aatgcatttg atcaaagaat atggtgcgtg gcgttccaga gaactcgttg gtttttatga
    15061 aaatctgtgt cgagtcatct tcaatcgcta taaaggactt gtcaaatatt ggctgacatt
    15121 caatgagatc aatatgatcc ttcatgcacc attcatgggc gcagggctgt attttgaaaa
    15181 aggagaaaat gaagaacaaa tcaaatatca agcggcacat catgagttgg ttgcaagtgc
    15241 catcgcaacg aagattgctc atgaagtaga tccggagaac aaagtaggct gtatgctggc
    15301 agcaggagca tattatccat acacttgcaa tcctgaagat gtttgggctt ctagaaaatc
    15361 agatcgagaa aattatttct tcattgatgt ccagtcacga ggagaatatc ccgcttatgc
    15421 attgaaagaa atggagcgaa atggcattca gatcgaaatg gaaccagggg atgccgcatt
    15481 gctgaaagaa catactgttg attttatctc tttttcttat tatgcttccc gagtttcgac
    15541 taccgacaac gaactcttgg aacagactgc aggaaatatc tttgcttctg tcaaaaatcc
    15601 atatctgcaa gcaagtgaat gggggtggca aattgatcca ttaggcttga gaataacgat
    15661 gaatgatatt tatgatcgtt atcaaaaacc attgtttatt gtagaaaacg gcttaggtgc
    15721 agtcgatacg ccagatgaaa atggttatgt ggaagatgat taccgaatcg attacctggc
    15781 agcgcatatc caggcgatga aggatgcagt agaactagac ggagtcgatc tattaggcta
    15841 tacgacttgg ggatgtattg atttagtatc agctggtacc ggtgagatga agaaaaggta
    15901 cggattcatt tatgtcgatc gtgataacca aggaaatggc acattagccc gttcaaagaa
    15961 aaaatcattc aactggtata aaaaagtaat tgattcaaat ggagaaaatc tttcgaacga
    16021 ttgatcaatg atttataaaa gtctattgtt tttcagcgga tttttcgagt ataataaagc
    16081 tattcgagaa acgcaagcag aacaatagac tttttttcgc attggctgta aagtatgctt
    16141 acttgattaa gtaaaggaga atctacatat gtatgatgta atcgttattg gcgcaggtcc
    16201 tgctgggatg actgctgctt tatatgcttc acgttcaaac ttatccgtat tgatgatcga
    16261 acaaggtgcc cctggcggcc aaatgaacaa tacggcagaa atcgaaaatt atcctggatt
    16321 cgattcgatc atgggccccg atctagctta taagatgtat gaaagtgtct caagatttgg
    16381 gacagaaaat gcttatggaa tcgttcaaga tatcaaggat cacggcagcc ataaagaagt
    16441 catctgtgaa gataaaagtt acgaaggaaa aacagtcatt atcgcaacag gatgtatcca
    16501 ccgtaaatta ggcgtacctg gcgaggaaga atttgctgga cgaggcgttt cttactgtgc
    16561 agtctgtgat ggtgcgtttt tccgtaacaa acgtctagta gtcatcgggg gtggggactc
    16621 agcagtagaa gaagccatct atttgacaca atttgcttct gaagtagtga tcgtccaccg
    16681 tagagatgaa ttacgtgcac aaaagatcat ccaagaccgt gcatttgcaa atgaaaaaat
    16741 ttcctttatg tgggatacag tcgttgacga aatcgttgga aacgaaatgg tagtgacagg
    16801 tgtgaaagca cgtaatgtca aaaccggcaa tgaatttgat ttagcagcag acggagtatt
    16861 catctatgtt ggactggatc cattaactgc accttttaga gaagcaggtt tgacgaacga
    16921 agctggctgg attccaacag acaacgaaat gaaaacgaat atgccaggcg tttttgccat
    16981 cggagatgtt cgtgaaaaag atttgcgaca aatcactaca gctgttggtg acggcggtat
    17041 tgctggacaa caagtgtata aatacttgga gtctttagaa gaaacaacag cataagttta
    17101 aaaagagtag aagaacgggt tcaattgttc ttttactctt ttttttcgcg tgaaatttac
    17161 tgaatctgat ttatgcgaac acgtccaaca ccttttgata aaagaatcct ttcttgattt
    17221 aagcgttttt tggtcctcat actttcatat ctaataagtg aatgctatac ttattaagaa
    17281 ccatatttca gaaagaggtg ttattttcat gtcttgggaa caagtgtatg aacaatgggt
    17341 acatgaagaa aatttagaag aaaacttaaa aaaacaatta aatgatctgg gtgaagatcc
    17401 tgaaaaactt gaagatgctt tttatgcacc gttggaattc ggtactgcag gtatgcgggg
    17461 aatcttagga cctgggatca atcgaatgaa catctatacg gtaagacaag caacagaagg
    17521 actagcacgt ttcatggata cacaaggtga agaaacaaaa cgccgaggtg tcgctatcgc
    17581 ttatgactca cgccatcagt caccagaatt tgcaatggaa gcagccaaaa cactagcaca
    17641 tcacaatatc ccgtcttatg tattcgaaag tctgcgtcca acaccagagc tttcttttgc
    17701 agttcgtcat ttgaagacgt ttgcgggaat catggtaacc gcatctcaca acccagcggc
    17761 ttacaatgga tataaagtct acggagaaga tggcggacaa atgcctcctg cagacgcaga
    17821 tgcattgacg aaatatgtcc gtgaagtcag caatccgcta aaagtcgaag tattatctga
    17881 cgaagaagct aaacatagcg ggttgatcac gatcatcgga gaagaagtgg acgctgctta
    17941 tctagaagaa atcaaagcag tgacgattga tcgtgaattg gttgaaacaa tggggaaaga
    18001 tttgaaactt gtgtacacgc cacttcatgg aacagggaaa atgttgggcg aacgtgcgtt
    18061 gaaacaagct ggattcgaac aattcgtact agttcccgaa caagctgtgg cagatccaga
    18121 tttcactacg gtcaaatcgc caaatccaga agaacattca gcttttgaat atgctatacg
    18181 acttggagaa aaagaaggag ctgacttgct gattgcaaca gatcctgatg cagaccgttt
    18241 aggcgcagcg gttcgtttgc cagatggttc ttatgaagta ttgactggga accaaatcgg
    18301 agcattgttt gctcaatata ttttggaagc acatcaatca agaggcactt taccagaaaa
    18361 cgcagcggta ttgaaatcaa tcgtttccag cgaattgcct acagcaattg cggaacacta
    18421 cggtgctacc atgttcaatg tgttgacagg gtttaaattc attgctgaaa aaatccaaca
    18481 atttgaagaa gaccattcac atacttttat gtttggattc gaagaaagtt atggttattt
    18541 agttaaacct tttgttcgcg ataaagacgc gatccaagca ttgcttttat tagcagaagt
    18601 agcagcttac tataaaaaac aaggcaaaac gttgtatgac ggcttgcaaa cgatttttga
    18661 aacctatggc tatttcgcag aaaaaacgat ttctgtgaca atgagcggac aagaaggttc
    18721 tgcgaaaatc tctgctttga tggaaaaatt ccgtaaagaa gcaccgacag aatttgccgg
    18781 agcaaaagtt attcaaacag aagactttaa agaattgacg agaacagacc gtgaaggaaa
    18841 cgtagaaaaa ctaacaacac cgccatccga cgtattgaag tatacattgg aagaaaatgg
    18901 ctggatcgct atccgccctt caggaactga gccgaaaatc aagttttata ttggtgtaaa
    18961 aggaagctct acagaagacg cagccgaaaa agtagcctct tatgaagcga ttatcaaaaa
    19021 gatgacagaa gaataaaaaa cttgagactg atggaaaaag tggtaaactt tagaagtcag
    19081 tcttttttca agttggaggt gaatggattg ccagacacgc aaaaatcaaa agtagagaaa
    19141 gtcagtcaat tattcaaaat gttgagtgat cccactcgtc ttaaaattct gctatatctg
    19201 aaagacgggg aacaaaatgt gacagcaatc acccaagcag tggaaatgga gcagtctgct
    19261 gtatcgcatc aactacgttt attaagagaa aatcatgtcg tcaagtctca tcgcgaggga
    19321 aaagcgattt tgtatagtct tgatgattct catgtactgg atattttgaa ccagacactc
    19381 aaacatgtgg aacatcaata acgataggtg aaaatagata aacactggag tgtgacaacg
    19441 aagaaatttc gttgtcacac tccagtgttt tttaagaaaa agacaaaaat gattaatcgc
    19501 ttttttctgt tgtttcagtt ccataataac tatgatcttc taggtctaaa gcattcaaaa
    19561 taagcgaagc cgtttcttca atagataaag atgccacatt gatcactaca catcctagtt
    19621 tttcataaag atcattcgca aaatcgagtt cagctctaat tttttcgata tcggaataag
    19681 cagtatcagg gtttaatccg taagagcgca tccgttcttt acggatatta tttaaaatat
    19741 ctgggttatt cgttagtccc acgatttttt taggatcgat ttcccacagc tgtttgggaa
    19801 tatgtgcttg gggaatcaaa ggtaaattag caacttttaa atttttattt gctaaaaaca
    19861 agctcaaagg cgttttggaa gttcgggata cgcctagcaa aacgacatct gcttctaaaa
    19921 aaccacgagg atctttccca tcatcgtatt ttacagcaaa ttccattgct ttgatccgtt
    19981 tgaaatagtt ctcattcaat aaatggaggg caccaggttg ccgaattggc ttgataccag
    20041 ttaaacgttc aatttctgat atcggtggcg tcatcacatc gaaactaaat aattgatgtt
    20101 cttcacaaaa agaacgtgtt atctcgatca gttcttgatt gattaatgta tgcaggacaa
    20161 gtgctttttc ttctaatgca tcttgcaatg cctgccttaa tgttttttca tctgtcacga
    20221 acgttctgcg gaataggttg aactcaacgg taggatactg agccatagta gcttgcgcta
    20281 gttttgtcgc agtttctcca gcagagtcgg agatgacaaa aaaagtaacg atttctgtag
    20341 aatcggaaga catggataaa acctcacttg catttttttt atgtttatta taccatactc
    20401 tagaatgcgt acttcgtaat cattttgatt tggaggaata agtcatgaaa aaacagacat
    20461 tgatcgaaca atcattagaa acattgaaaa ataacggttt taaatatact aaaaaaagag
    20521 aagcattgtt aacttattta gtgaagcgta accgctacgt atctgctaaa gaagtttttg
    20581 actatatgaa tcaggaattc aaaggggtaa gttatgatac gatttaccgg aatctccatg
    20641 attttgcaga gatcgattta ctggaagaaa ccgatttaaa tggtgaaatg aagtttcgtt
    20701 ttcattgctg tcagggggaa atcggccatc accatcattt tatctgtaca gtgtgcggaa
    20761 caacgaaaga aatccatatg tgtccaatga attatttcga agaacagcta ggtggatgca
    20821 cgatcgaagg gcatagattc gagattttcg gaagatgtga aaattgtcaa taaggattta
    20881 aataaaataa catttgataa tttaaaaaaa aagcaagatt tccttgacgg tcaaaacagc
    20941 attagatata atatttacag gacagtcttg ttttttgaga tatatttcaa taacaattac
    21001 ggttactttt taagctcgga gggagggaat taacatgtca aaaacagtcg ttcgcaaaaa
    21061 cgaatcactt gacgatgctc ttcgtcgctt caaacgttcc gtgtcaaaag cgggtacttt
    21121 acaagaatct cgtaaacgtg aattctacga aaaaccaagt gtaagacgca agaaaaaatc
    21181 tgaagcagct agaaaacgca aaaaattcta gttctgtgat gaattggagt tggtagtgtg
    21241 tcactactaa gtagattaaa cgatgacatg aaagcagcga tgaaagcgaa agataaggaa
    21301 tctttacaag tcatccgaat gatcaagtca tctatacaaa acgagcagat taaagaaggt
    21361 cacgacttga cagaagaaga agaactaacg gtgttgtctc gcgaaatgaa acagcgacgt
    21421 gattctttgc atgagtttga agaagcagga cgtgacgatt tagctgaaaa agtaaaaagt
    21481 gaaattgtaa tagtcgaaaa atatatgccg gaacaacttt ccgacgaaga aattcgacaa
    21541 cttgttcaag aagcaatcac tcaaactggt gcttcatcaa tgaaagaatt tggcaaagtt
    21601 atgggtgcaa ttatgcctaa agtcaaagga aaagcagatg gaaaccaagt gaatgcgatt
    21661 gtgaaagaac tattacaatc ttaattttat catttaaact gcggacagtg attgtccgca
    21721 gttttttgtg ttcgtaaaaa aagaactaaa aaacagctag aagaaagaat aagattcttt
    21781 cttctagctg tttttttcct gtttaatatc tcttatgtta aattgtgttt gatttggtta
    21841 ttcttctggt agttcatttg cttcttgttt ataccctgat aggcgatagg cgtaataaaa
    21901 gagtaagctg ccagcgtaac caagtaacgt tgggaccatc cagccaagtc ctaatgcagc
    21961 aaaaggaacg tgatgatgat aaaatcctag taagcttgct acgacagttt gctgactcac
    22021 taatgcaggc gaatttgcca acatatccaa tacggctgga acggcagaaa agaacatcgt
    22081 tacttgatag acaaatggac tcttttggaa gaattttgct gttaaggaaa gcaagattaa
    22141 cgcaagtgac aatggataca aaagcatcaa tactggtaaa gaccattgga tgatattatc
    22201 caatccggca ttcgcaacga caaaagagac aaaagtcgtt aaccgcagcc atgtcatata
    22261 actgacttta gggaacagtt tatggaaatc ttgtgcaaaa gaaacaacga gtcccatagc
    22321 ggtagtgaag acacctaatg tgaccattac ccctaagaag ctagcaccga agttacctaa
    22381 ataaaaatta gtgatctgag aaagtgcagt tccgccattt tctgataaag gcatcttatt
    22441 caaactcatc gcaccgagaa ggaccagacc aaaataaatc atgacttcta acgcaacgct
    22501 caggctacca gcttttgctg ttaattctgt gactttttta tctttaaaac caagatcacg
    22561 aactgcatgg acaaaagtga cacttaaagc aagtaaagct actgcatcaa ccgtgttgta
    22621 tccttctaaa acgccatttg tcaaagcgtt cgtctgataa gaatctgcag cagattggtt
    22681 tagattcccc atgggttgga ataaagaaac gacaaaaatg atagctaata atgcaagaaa
    22741 caaggagttt aaatactttc cgacatattt gaccaaacgt gttggatgta cagtaaggaa
    22801 ataagcaagt aaaaagaaaa gagcagagaa taaaaacata cctaatgaat catatttttc
    22861 aggcaaaaag ggtttagcag ccatttcaaa agcagttgct gctgtccgcg gtgtaccaaa
    22921 gaatggtccg atcgttaaat gaacaaggat caagaacaag gcagcatacc attttccgac
    22981 aggtttagct aagtcataca gaccatcact ttttgtcact actacagcta agatagctaa
    23041 taatgggaaa agagcgcctg agatggcaaa tcctacacca gcgctgatcc agtttgatcc
    23101 agccatttga cctaagtgga caggaaagat cagattacct gacccgaaga acatcccgaa
    23161 gataagtgaa ctgaccatca ataattgctt ccatgaataa gtttttgttt cttccataaa
    23221 tttttcctcc aataattatc attttgagta acaaaataaa cgttgcagaa actagaaatg
    23281 ccactaatat cagattccat gaaaaccacc accttaaaat ataaaaaaaa gcccttttcc
    23341 acttgggaaa agggctctag attctagaac ggtaccacct tttttcgctt aaaaaagctt
    23401 cttcattcac ggacgctttt cgataaagtt atccgctagc ctgataatgg aggcagccac
    23461 aatgatctac gttttcgaca ttgactcgga aatgatgttc gtctattcga tttattgtca
    23521 tttctcactt acatgactca ctgggaataa atcgctgaat agcctactgt tttcctcgac
    23581 gctttgctgt atatttattt gttgacgtta taatacacct gttttttttg aaatgcaaga
    23641 atatttcaaa tattttgttt tctttttttg aaacaagaaa aatacccaga gaatacttgc
    23701 taagttagac ggaatacttg tcctctatct tatgcatagc ttacatgatc agcttttttt
    23761 attcaaacga gacctttaga gaatggaagc agctattttt taacgtttct tggtacttat
    23821 ggttaagcaa ctcttttaga accttttctt atttgctata tagtttcttc tgagtatggt
    23881 aaaataggaa agaatgaatc gaaaaaaagg ggatgtctat ttttgacgga agagtctagt
    23941 tctttagaga ttagattgac ggctgctgat gatgtcagca tgcttttagg tgcacacgac
    24001 aagcatataa aactgattga agaaacaaca gaaacaacca tccatacacg tggagaaatc
    24061 attcagatca ttggcgaaca atcccagatt tccttggctg cctcagtcat tcggacattg
    24121 caggaattga tcaaacgagg aatccatgta agtacaccag atgtcgtgac ggcactgaaa
    24181 atggggcaaa aaggcacatt agattatttt gttgaaatgt atgaggaaga gatcgtcaaa
    24241 gaccgtaatg gaaaaccaat acgggtaaaa aacagcggcc aaaagaaata tgtcgaatcg
    24301 gtagccaaac atgatatcgt atttggcgta ggaccagccg ggacaggaaa aacttttctt
    24361 gcagtagtat tagcaatcgc tgccttgaaa aaaggtgaag tgcaaaaaat cattttgacg
    24421 cggcctgctg tggaagcagg ggaaaatcta ggctttcttc ccggggatct aaaagaaaaa
    24481 gtagatcctt atctgcgtcc tgtttatgat gctttatacc aaatttttgg attagatcat
    24541 acgaaccgat tgatggaacg aggcgtgatc gaaattgctc ctttagctta catgcggggg
    24601 cggacattgg atgatgcatt cgttattttg gatgaagcac agaacacgac agttgctcag
    24661 atgaaaatgt tcctgacacg tcttggttat caatcaaaaa tgattgtcaa tggagatacg
    24721 agtcagattg atctgcctcg agggacaaca agtggtcttg ttcatgcaga acgaacatta
    24781 aagcaaatca agaaaatcga ttttgttaat tttgaagcaa gtgacgttgt tcggcaccct
    24841 gtagttgccg aaatcatcaa agcttatgaa aaggcagatc tccatcaaga ataagctttg
    24901 cttaggagga taagcacatg ttgaataaaa cactgcagaa actacaagaa aaacttggaa
    24961 aggcagcatt gcctttcctg ctgttcttgt tttttatcat tatctgcggc gttacattta
    25021 gtagtgtcaa acaaaaggcc aatgatttca gagaaggcca agtagcagaa gaaagtatcc
    25081 gtgcgaacaa aacgatcgag aacacggaag aaaccgaaca aaaaagaaaa ctggcagcag
    25141 aagcagtcac tccagaatac acttaccaaa aagatcttgc agatgaccag aacaatcgga
    25201 tcaagcaact atttgaattg atcgataaaa cgaatgatgc catcaacaaa tcttatgacg
    25261 aaaagattga taaggcaaag gaagatgaaa atatcccaaa gcctactaca gaagagcgga
    25321 tcgccgcgtt gaagaaaaat tttgaaaatg tgaatgcaga aaatgttgcg ttttatcaaa
    25381 aactaccgaa caatttttat tcgacgatat ttgaaatgac tgaaactgag atccatactg
    25441 tgagggatga gagtctgaag ctcattgacg aacagatgag caaacaagtt cgtgaaagtg
    25501 aattggaagc ttttaaacaa gaagcagaag agcaaatcca atatttgaat gtcacaccag
    25561 cacaacaaca aatgattcgc tacttagtgg atcaaggtat cgtagtgaat gacgtattga
    25621 atgagaaaaa gacagaagaa ttgaaacaat ccgcacgaga agctgtacaa ccagtgatga
    25681 tcttccaagg agaaatcatt gttcgtgaag gtaatcaaat cgatgcttca gctatgaaaa
    25741 aattggaatt actaggactg actaatcaaa caacttcgat ttttccgcta attgccatgg
    25801 ttttagctgt tttgcttcaa atcggcgtac tgatttataa ttcacttcaa ttccaaaatg
    25861 caggagaaag aacaaaatac gtgcttttct atgtgacagc tatgtcagtc agcatagtgc
    25921 tgatgaaatt tttccaatta ttccaaacgg aacaagctac ttatatcccg ctgttttatc
    25981 ccgctgcttt tgcaccactt gttttaagct ttttcttgaa ccgaagagca gggatcatgg
    26041 ctggtatttt ccaagcagta tctgcacttt tcatttttta tgattcgatt ggcacgaact
    26101 ttctaacgat tatcttgatg tcttatgttt tctctgggct catggcaacg attgtcagac
    26161 gcaaacggat ctcagaacaa ggattatctg ctgccatgtg ggtcattatt tttcctgtga
    26221 tgatggatgt cattttgatc gtctatcaag gaatgagctt tggtgatgca acgacttggt
    26281 taatgcttgt ctgcgggttc actggtgcac tcttctcatt cttgctgacg atcggtctac
    26341 acccatatat tgaattgatg gtcaatgatg acagtgtaat tgtattaaat gaactcagca
    26401 atccaaatca tcctttattg aaacagttgc tggaagaagc gccaggtact tatcatcata
    26461 gtatgatggt agctaatcta agtgctaacg ctgtagcaga aatcggcgga cgttctttgc
    26521 taactcgagt agcttgctat tatcatgata tcggcaagat caaacatgct agctttttcg
    26581 ttgagaactt gccttcagga gcggaaaacc cgcacaattt cttattgcca gaagacagta
    26641 aacatattat ctttggacat gtgacagatg gagccaaaat attggaagat tataatatgc
    26701 cgcaaatggt gatcgatatt tgcaaacagc atcatggaac cacgctaatg cgttatttct
    26761 acatcaaagc gaaagaacgc aatccggaag taacagaaga gcaattccgt tatcctggac
    26821 caaaaccgca aagtcgtgaa gcagggatcg tcaatattgc tgacagttgt gaggctgcag
    26881 tacgggcaat ggatcatccg acgagcgaaa aaatcgaaag ttttgtccat aatcttatcg
    26941 aagagcgtat ttccgatggt caattagacg atagtggact gaccttgaaa gaaatccgaa
    27001 aagtggaaaa atcattgatc agcggtttaa gtagcacctt ccattctcgg atcaaatatc
    27061 ctaaaatgaa aacagaagtt gagaagataa aggaagaaca agaagaatag aaagaagcga
    27121 tgtataatgg atattacctt tatggatgaa acaaaccaag tctcagaaga gaaaatgaaa
    27181 gaaatcgatg acttgctgca atttgcagca tcttatttaa agctgccaga ggaaacagaa
    27241 atgtctgtta ctttcatgga caatgcggca atccaagtaa tcaatcgtga ttaccgtgga
    27301 aaagatgcac cgacagacgt catcagtttt gctctggaag aagaaggcga agacgaaatc
    27361 ccagtgattt ttgaagatgg agagaatcca ttgcctcgtg tactaggaga catcatgatt
    27421 tctattgaac gtgctgctga acaagcagaa gcttacggcc attcatatga tcgggaactt
    27481 ggctttttag cggtacatgg attcttgcat atcaacgggt atgaccatat gacaccagaa
    27541 gatgagaaag aaatgtttgg tttacagaaa gagatcttgg atgcctatgg actcaaaaga
    27601 taaaaacgta gagaaaaaca aacatttcct gatgtctgtg gaatttgctg tccaaggagt
    27661 caaaaccgtt tttgaggaag aacgcaatat gaggaaacat gttgcgttcg gtatccttgc
    27721 tttatttatg ggcgtgattt tccagcttga tcggatagag tggttatggc tgcttttagc
    27781 tgtttttctc gtttggatag tagagatcat caatactgtt tttgaaaatg tagtggacat
    27841 gtttacggat tttcattttc atccgattgg gaaaaaaatc aaagatatgg cagctggagc
    27901 agttttagtg accgcttgtt ttgctgtcat cattgggttg attttatttg tccctaaaat
    27961 atatcatttg ttgtttaatt aaaaaggaga acgtatatgc cagaacataa atcagggttt
    28021 gtcgcaatcg tcggccgccc gaatgtagga aaatccacat tattgaaccg aatcgtgggt
    28081 caaaaaattg ccattatgag tgacaaggca cagacaacta gaaataaaat ccaaggagta
    28141 tatactacac cagaagctca gctgattttt atcgatacac caggaatcca taaaccaaag
    28201 catcgtctag gagattttat ggtagaagca gcttacagtg cgttgaaaga agtggatgca
    28261 gtcttattta tgatcagtgc tgatcaaaaa cgcggccggg gagacgattt tattattgaa
    28321 cgtctaaaaa acgtgcattc tccagtttat ctgattatca ataaaatcga taagatccat
    28381 ccagacgagt tgctgggaat catcgaagat tattcgacgc agatgccttt tgcacaagtg
    28441 attccgatct ctgctacaga aggaaacaat gtcgaacgct taatggatgt attagtcagt
    28501 gaaatgccag agggcccaca atattttcca gatgatcaag tgactgatca tcctgaatac
    28561 tttatcgttt ctgaattgat ccgtgagaaa gtcttgtttt taacacgaga cgaagtgcct
    28621 cattctgtcg cagtcgttgt tgattcgatg aagcgaaacg agaatgataa aatccatatc
    28681 caggccacga tcatcgtcga aagagatagc caaaaaggaa tcattatcgg caaaggcgga
    28741 aaaatgctga agcaagtagg aacgaaagca agaaaggata tcgaaaatct tttaggagac
    28801 aaagtatttc tagaactttg ggtcaaagtc caaaaagact ggcgagacaa aaaagtctat
    28861 ctgcaagact ttggctaccg aaaagacgaa tactgatatt ctgaacttct tataggttaa
    28921 gtatagaaat aaactggata aaaatccgca cgttgagaaa aacggcggaa gatcgaaaca
    28981 taaataatct ggatcggcag aatctgcttt tccagattta ttttttagga gggagaatat
    29041 gagccagctt gcagaatcga aaggcttgat tttatctagc cgtaattaca aagaaaaaga
    29101 taaattagtc aaaatcttta cggaatcttc tggcaaaatg atgttttata tcaaaggaat
    29161 ccatcggcaa aatcagccat tagctccggc tttgctcccc tttaccgaag cagtctatat
    29221 cggtaatttt cgaagtgaag gtttgtcgtt tttaagcagt gtcaaagacg tccatgcgtt
    29281 tcggaacatc caagaagata tctttttgag cggttatggt acctatttgc taaatctagt
    29341 agatgcagca atcgaagatc atcaatatga cccgcattta ttccaattta cgcaacaagc
    29401 tctgcagcgg atggatcaag gagatgatgc agaaatcatc acgaatatct ttgaagttca
    29461 aatactgcaa cgcttcggaa tcgcgccaat ttggacgcac tgtgtcgtat gcggggaaac
    29521 aaaaggaaaa tttgactatt cttccaaatc tggcggtgtg atatgcgaga aacactggcc
    29581 aatggacgaa caccgttatc atgcagatcc tcgcgctgtt tatttcgttc gaatgttttc
    29641 ggctatttct tatgataaga tcagtggaat ccacttgaaa gaagaaacga agcaggcaat
    29701 ccgtgacctc attgaccagt tgtatgatga atatgtcggt attcatctaa aaagcaaaaa
    29761 gttcattgat caaatgaagt cttgggcaca cgtattaaag acgccagaga aaaatatgcc
    29821 tgaaaaagag atacctgaaa aagaagaata aatgaaagat acaacaaaaa aatcaaaact
    29881 atttgacaaa tacagggact tccgtctatt attaaccgta gattaaatag atcgttcact
    29941 gagaggatct attatataaa caagaaaact gcaaaatgaa tacgtacatt gaccaagatc
    30001 agtaaaaaac acgtggttta gcgagttcgg gagagtggaa gccggaccca aacgattttt
    30061 gaagaggcac ttgcgagtac gataaatgaa gctgccgaat ctttattcgg aagtagggtg
    30121 gaaccgcgaa attattcgtc cctatgtctg taatcagaca tagggacttt ttgtcgtttt
    30181 ctttcctgta ttcttattga cagcaacgct taaaaattga aaggggcaaa acagatgagt
    30241 aaaaaattga ccgtccaaga tatgatttta acattacaaa aattttggtc atcaaacggc
    30301 tgcatgctga tgcaagctta tgacacagaa aaaggagcag gtacgatgag cccatataca
    30361 ttcttacggg caatcggacc ggaaccatgg aatgctgctt atgtagagcc atcccgccga
    30421 ccagctgacg ggcgttatgg agaaaaccca aaccgtttgt atcagcatca tcaatttcaa
    30481 gtcgtaatga aaccttcacc tgaaaatatc caagagcttt atttagaaag cttgcgtctt
    30541 ttaggaattg atccattaga acacgatatt cgttttgttg aagacaactg ggagaaccca
    30601 tcgatggggt gtgccggtgt gggttgggaa gtatggcttg atgggatgga gatcacacaa
    30661 ttcacgtact tccagcaagt cggaggacta ccttgtaaac cagttacttc tgagatcact
    30721 tatggactag aacgtcttgc ttcatatatt caagaagttg aaagtgtcta tgatctggaa
    30781 tgggctgatg gtgtgaaata tggcgagatc ttcaaacaac cagaatatga acattccaaa
    30841 tattcttttg aagtaagtga ccaagacttg ctactaagca actttgaccg ttttgaaaaa
    30901 gaagctaaac gctgtatcga tgaaaatctt gttcatccag cgtatgatta tattttgaaa
    30961 tgcagccata cattcaattt attagatgct cgcggtgcag tatccgtaac agaacgagca
    31021 ggttatcttt cccgtatccg caacatggct cgtgctgtag ccaaaatctt tgtggcagaa
    31081 cgcgaaaaat taggattccc tttattgaat aaaaacacag caaatccaga aaacatgaag
    31141 gaggctgaat aagatggcaa aagacttatt acttgaaatc gggctggaag aaatgcctgc
    31201 tcatgttgtc acaccaagca tgaaacaatt agaagaaaaa acagcaaaat ttttagatga
    31261 aaaccaattg acttatgata cgatcgaaac gttttctaca ccgcgccgct tagcaatcaa
    31321 agtaacaaac attccagaac gtcaagcaga cagtgaagaa gaagtcaaag gaccagccaa
    31381 aaaaattgct ttggacgcag atggaaactg gacgaaagca gctgaaggat ttgtccgcgg
    31441 acaaggtctg acgacagatg ctatcacttt tagagaatta aaaggcgtgg aatatgtcta
    31501 tgtaacgaaa tacactcacg gcaaagaagc gaaagaagta ttgacaggtt tagcagatgt
    31561 cattaaaagc ctcactttcc cagtaacgat gcattgggca gaatacgatt ttgaatatat
    31621 ccgccctatt cactggatcg tggcattgtt agatgacgaa gtaatcccgt ttgagatctt
    31681 agatgtcaaa acaggcagag cctctcgagg acatcgtttc ttaggcgaag atgtgacatt
    31741 tgcgcatgca aaagaatatg aagaaaaatt gaatgaacaa ttcgttattg cttcaccgca
    31801 aaaaagacaa gaaatgatcg ttacacaagc aaatgaatta gccgaaaaaa atcactgggt
    31861 tattgatcta gacgaagatt tactagaaga agtcaacaat ttagtcgaat atccaactgt
    31921 cttttctggc gacttcgaag aaaaatatct ttctgttcct gaagaagtac tagtcacttc
    31981 tatgaaagaa catcaacgtt atttcgaggt acggaacgat caaggtatgc tgcttcctca
    32041 ttttatttct gctcgtaatg gtgatcgggt aaaattggaa aatgtggttc gaggcaatca
    32101 aaaagtattg acggctcgtt tagaagatgc ggaattcttt tataatgaag acaagaaagc
    32161 ttcaatcgac caatacgttg aaaaactaaa aactgttact ttccatgaaa aaatcggtag
    32221 tatctatgag aaaatgcagc gggtacaagt cattgcccgt atcattggcg aacaagtagg
    32281 actatctgag gaagaattga tggatctgca acgagctgct gaaatctata aatttgatct
    32341 agtaaccaac atggtagggg aatttcctga actacaaggg atcatgggcg aaaaatatgc
    32401 tctgctgaaa ggcgagaaac ctgctgtagc aacagcgatt cgtgagcatt acatgcctat
    32461 atccagtgaa ggagagctac ctcagtctac agtaggttct gttttagcga ttgcagataa
    32521 attagatagc attttttctt tctttgcagt tgggatgatt ccaagcgggt caaacgatcc
    32581 ttatgcctta cgtcgccaag catacggtgt cgttcggatc atcgaatcga aaaaatgggc
    32641 ctttcctttg tctgttctgc aagaaacgat tagtgatgtt atcagtaaag atacggatcg
    32701 tttcggcatt ggtttatcag aagggcaaca acaggtgatc gactttatca aaggccgtct
    32761 gcgccaatta ttgacaacga agaatattcg tcatgatgtc atcgaagctg ttttgaatgc
    32821 agaacaaaaa gatctgacaa aagtatttgc agcagcacaa ttgttcaagc agcatcttgc
    32881 agatgaagac ttcaaaccat cgatggaagc tttgacacgt gtcgtgaact tagcgaaaaa
    32941 agcggagctt gaaaaacaat cagaagtaga ccctgaactg ttcgaaaatg aagcggagaa
    33001 agaactgcac aaagcagtag aggcaatgaa aaaaagcttt gctgaccaaa cgatcaacga
    33061 aaattatgat cagcttgtaa gccttcgccc actgatcgaa aattattttg atcagacgat
    33121 ggtcatggca gaagaaccag cagtacgtaa gaaccgatta acgcaattaa gtcaaattgc
    33181 tgccatggct ttgtcgctag caagtttaga tcaaatcatc acaaaataat ttctaatatg
    33241 taaataaacc agagaatagg cacaaaa
//
LOCUS       DACDMM010000012        32867 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDMM010000012 DACDMM010000000
VERSION     DACDMM010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.012"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(179..1510)
                     /locus_tag="IWD16_000441"
     CDS             complement(179..1510)
                     /locus_tag="IWD16_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9087527.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(1659..2372)
                     /locus_tag="IWD16_000442"
     CDS             complement(1659..2372)
                     /locus_tag="IWD16_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9087528.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            2547..3971
                     /locus_tag="IWD16_000443"
     CDS             2547..3971
                     /locus_tag="IWD16_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9087529.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            4270..4977
                     /locus_tag="IWD16_000444"
     CDS             4270..4977
                     /locus_tag="IWD16_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP9087530.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            4964..5287
                     /locus_tag="IWD16_000445"
     CDS             4964..5287
                     /locus_tag="IWD16_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9087531.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(5376..6413)
                     /locus_tag="IWD16_000446"
     CDS             complement(5376..6413)
                     /locus_tag="IWD16_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9087532.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(6734..7717)
                     /locus_tag="IWD16_000447"
     CDS             complement(6734..7717)
                     /locus_tag="IWD16_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP9087533.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(8132..8902)
                     /locus_tag="IWD16_000448"
     CDS             complement(8132..8902)
                     /locus_tag="IWD16_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9087534.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(8895..9830)
                     /locus_tag="IWD16_000449"
     CDS             complement(8895..9830)
                     /locus_tag="IWD16_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9087535.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(10146..11795)
                     /locus_tag="IWD16_000450"
     CDS             complement(10146..11795)
                     /locus_tag="IWD16_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9087536.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(11912..14515)
                     /locus_tag="IWD16_000451"
     CDS             complement(11912..14515)
                     /locus_tag="IWD16_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9087537.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(14527..15576)
                     /locus_tag="IWD16_000452"
     CDS             complement(14527..15576)
                     /locus_tag="IWD16_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP9087538.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(15602..15916)
                     /locus_tag="IWD16_000453"
     CDS             complement(15602..15916)
                     /locus_tag="IWD16_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP9087539.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(15932..16378)
                     /locus_tag="IWD16_000454"
     CDS             complement(15932..16378)
                     /locus_tag="IWD16_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9087540.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(16379..18355)
                     /locus_tag="IWD16_000455"
     CDS             complement(16379..18355)
                     /locus_tag="IWD16_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP9087541.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(18513..19409)
                     /locus_tag="IWD16_000456"
     CDS             complement(18513..19409)
                     /locus_tag="IWD16_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9087542.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(19402..20082)
                     /locus_tag="IWD16_000457"
     CDS             complement(19402..20082)
                     /locus_tag="IWD16_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9087543.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(20083..20865)
                     /locus_tag="IWD16_000458"
     CDS             complement(20083..20865)
                     /locus_tag="IWD16_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9087544.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(20858..21775)
                     /locus_tag="IWD16_000459"
     CDS             complement(20858..21775)
                     /locus_tag="IWD16_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9087545.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(21939..22262)
                     /locus_tag="IWD16_000460"
     CDS             complement(21939..22262)
                     /locus_tag="IWD16_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9087546.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            23050..26277
                     /gene="fss3"
                     /locus_tag="IWD16_000461"
     CDS             23050..26277
                     /gene="fss3"
                     /locus_tag="IWD16_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP9087547.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            26390..26704
                     /locus_tag="IWD16_000462"
     CDS             26390..26704
                     /locus_tag="IWD16_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9087548.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            26717..27091
                     /locus_tag="IWD16_000463"
     CDS             26717..27091
                     /locus_tag="IWD16_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9087549.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            27101..27436
                     /locus_tag="IWD16_000464"
     CDS             27101..27436
                     /locus_tag="IWD16_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9087550.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            27519..28859
                     /locus_tag="IWD16_000465"
     CDS             27519..28859
                     /locus_tag="IWD16_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9087551.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            28937..29611
                     /locus_tag="IWD16_000466"
     CDS             28937..29611
                     /locus_tag="IWD16_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9087552.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            29844..30278
                     /gene="dcm"
                     /locus_tag="IWD16_000467"
     CDS             29844..30278
                     /gene="dcm"
                     /locus_tag="IWD16_000467"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9087553.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            30279..30986
                     /locus_tag="IWD16_000468"
     CDS             30279..30986
                     /locus_tag="IWD16_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9087554.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            30976..31266
                     /locus_tag="IWD16_000469"
     CDS             30976..31266
                     /locus_tag="IWD16_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087555.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            31525..32709
                     /locus_tag="IWD16_000470"
     CDS             31525..32709
                     /locus_tag="IWD16_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9087556.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            32724..32843
                     /locus_tag="IWD16_000471"
     CDS             32724..32843
                     /locus_tag="IWD16_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9087557.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT        10171 a         6341 c         6020 g        10335 t
ORIGIN      
        1 ctaaatagac gtatatacac agataggttt gtcttgtgaa aaataatgat tatccttacc
       61 aaaaaatgtt tgtttttcta catgttcgta cttatcaaaa aatatgatcc ttattgaaat
      121 tactggctat ttatattcca acgacttctt gtgttaattt ttgttatctc atagtaaatt
      181 atgtttcatt ttcacttaat ttagttgtca cgtgttcgtc aattcgtaaa aacggtaaat
      241 atataaacac acttgcggct ataattaata tttgtaaaac agctgctcgc cagtctccgg
      301 ctgttgctaa aaatcccgat ataactggtg gcgtagtcca aggaatttgc actactacat
      361 gattaattaa tcccacagca gttgcaaaat atgctactgt caaagaaaaa atcggtgcaa
      421 gcacaaaggg aataatcaga ctgatattaa atacaatagg caatccaaaa ataataggtt
      481 cgcttatatt gaatatagcg ggagttaccg ataatttagc aaagtcctta taatcttttc
      541 tacgactgaa gataaatatt gcaatcaata aagcaatcgt atttcccgat ccacccatta
      601 ccgcaaaggt atctttaaag gccatattga taatatgtgg aatttgctct ttgtttgcat
      661 aagctgccat attttcttgc atattctgaa tcaaaaacgg atctaacaat gaacctgata
      721 ttactgcttg atggatacca aatccaaaaa atagattagc aatagatgta attaacaaaa
      781 atcctggtaa accagttgta attttactta atggctctgt aactatcgtg gtaattagct
      841 tatttatatc catattaaac aattgaatga ttataaatga caataaagca aacgaggtaa
      901 tcgtaataat gattgggatt aatacattaa aagactttaa aacagcggga ggaatatttc
      961 cggatagctg aatctgtagt ttcttattat tagaaagttt aataaataat tctgtagccg
     1021 ctaatccaac aaatatacct acaaacattc ctgaagctcc tgtataggat ataggtatca
     1081 cacccgtcac ttcaacagct ttatccgcat tttcaggagt aaacatcatc tgcataggtg
     1141 ttaatacaac aaatgtcgaa atagcaacta ataccgctgc aatatgattt gtataatttc
     1201 tgcttgcaca catatgataa gatactgctg ctacaactaa taatgtagtt atgcttaatg
     1261 taccattggc aattgaagta ccaatttgtt gtaacgtggt caatgtacta gtatcgacaa
     1321 tttcccccat gaatcctgtt ggttctaaga tcactgaatt aataagtgtc acaaatcctg
     1381 ctaacatcat aaatggcata aatgtagcaa acgcatctcg caaagttctt aaatggatct
     1441 gattccccaa cttagtcgca actataccaa atttttcaaa tacaatgtcc ttcctgctac
     1501 ttttgcccat attttttctc ctttatgctt attgtaatcg attacttttt ccacccaaat
     1561 aatacatcac ttatcaaaaa aagtaaatac ttttaattaa atatatatat cttttttagt
     1621 ataaaaaaat aagctctgaa aaaataattt tcccgagctt atttttcaac aaaaacagct
     1681 tcaaaaacaa agtcttgata taaatgtctc gataccgaat actcgaaagg tttaccagac
     1741 gatagagcaa caactttgtc tatctcaatc ataaattcac ctggatctat ttttaatatt
     1801 tttgcgtcaa attcattcgc agtatctcct ttgatacgta tgtgcgaagt ctttatttcc
     1861 agacccagtt catttgtgat ataagcatat acagatttct ttaaatgttt tggttccaag
     1921 cctggtatga ctgctaatgg catgaatgtt tgctcaatcg agtatggttg acaatcaata
     1981 aaacggcttc gtacaatatt atatacatag tcttttacac tgatacttaa tttatcagct
     2041 acttttttcg atggaagttc aattgagaaa gtatggattt ctgaatctac gtgttcttca
     2101 ccaaccacgc taaacaaacc ttcgtttgga ccaagaactc gtgagttatc aattggtgtt
     2161 ctgacgactt cagtcccata cccacttcta cttcgcaaaa ctccttcgga aacaagtaag
     2221 tctacgcctt tctttactgt taacttgctt gtatgataaa taactgccaa ttcatctcct
     2281 gtaggaagtt tctcaccaat tttatactct ccatttacaa tttttttctt tatatctaag
     2341 taaatttcca tatacttcgc aatttttttc aatggtgacc ctccaatcaa aaacatatct
     2401 tcatctatac tttaacataa ataaaccgat aaaatccaca tcaaaattat acacacaata
     2461 aaaaagatat atatatttaa acaaaactat ttactttaag ttgtaagcgg tgttatcatt
     2521 aagtggaaac aggaggtaaa aaaattatga caactatgaa aaagctaact tttccagatg
     2581 atttttggtg gggttccgct tggtcagctg agcaagcaga aggaaaagga gaaacaggaa
     2641 aagcagagac tatctgggat cgctggttta aagaacaacc ttaccggttt tatcaacgaa
     2701 tcgattcctc tattacaacc gatcacatcc atcaatataa agaagatata gaactaatga
     2761 aaaaaactgg gcataactcc tttcgcgttt ctatttcttg ggcacgaatg tttccaaatg
     2821 gcggtaccgg aaaagtaaat cccaaagcca tttctttcta tcgaaaatta ttttccgaaa
     2881 tgaataataa cggaatcaaa gtatttgcca acttatatca ttttgacatg cctgctactc
     2941 ttcaagataa aggaggatgg gaatcacgtg aagtagttga tgcttatgtt cattacgctg
     3001 aaacttgttt taaagaattt ggtgacttag tttgtcattg gtttaccttc aatgaaccac
     3061 ttgggcctat tttaggtact tatcttgaag attttcatta tccaaacctc attgacttta
     3121 aacgcggagc gcaagcagca ttcttcacta tacttgcaca cgcaaaagca atacaagtat
     3181 ttaaagaact agctcttcct agtaaaatag gcgttattct aaatttgagt cctacttttc
     3241 ctcgtagtca aaatcctgct gatgtgaaag cagcagaaat cgctgattta ttttatacgc
     3301 ggagcttttt agatcctatg gttaaaggga atttttctaa aaagcttatc ctgttattac
     3361 aagaatataa tcagcttcct gaagattata cgaaagaaga tcttgtatgt atctacgaaa
     3421 ataccgctca aattcttgga ctaaactatt acgagccacg tcgcgtcaaa tctcgttctt
     3481 ctatggtgga tacatctagt ccatttttac cagagtggtt tttcgaacca catatcatgc
     3541 ctggacgacg aatgaacgaa taccggggat gggaaattta cgaaaaagga atttatgatt
     3601 tgtgcatgga tatcaagaac cattatggaa acatcgaagc atttatctcg gagaacggaa
     3661 tgggcgttgc ggatgaagaa cgtttcatgg atgatacagg gcaaataatt gatgattatc
     3721 ggattgagta tatcaaagat catcttgctt acttatggaa ggcaataaat gaaggatgta
     3781 acattaaggg gtatcatctt tggacattta ttgattgctg gtcatggatc aacgcctata
     3841 aaaaccgata tgggctggtt tcactcgacc ttactactca aaaaagaaca attaaaaaaa
     3901 gtggcgaatt ttacaagcaa ttaagtgata caaatggatt tagatacgat gtagacttgt
     3961 tagttgaata atattctaaa attccacttg aagtgaacac attagaaaac aagaataatg
     4021 agaaatacca ataacaaata ctttttcttc atacttcatt tatacttgtc agtataagag
     4081 actgtgacaa atgtagtgat cctcagaagt taggtctgga aatctaactt gagaagattt
     4141 ttttaaacta cgtccagtct tttttatttg tttaaaattg aagttgacaa gtcttcatta
     4201 aaaatttaga atattcacaa gtttgacgac aattatgaaa atttatgtcg tgctaaagga
     4261 gttttgttat tggatgaaaa gttagatata aaaactgcga tcaaagacac gttacctacc
     4321 gtttttggtt atatcggtat tggacttgca tttggtatcg ttgggaaagc tgccgggttt
     4381 catccgctgg tcgtcacgtt gatgtccctg ctgatctatg ctggttctgc ccaatttatc
     4441 acggtcagca tgcttgctag ccatagtcca ctgctctcca tcgttctctc gacctttcta
     4501 gtcaattccc gaatgattct gatgagtatg acgattgctc cttatttcaa gaaaaatcgg
     4561 ctgcttcaaa atctgctgat tggcacattg ctgacagatg aaagtttcgc actgggaatg
     4621 aataaattga actatactgg acaaaagtta aactttcgtt ggatgaatac agctaattgg
     4681 atttcttatt tgacgtgggt gggttcttct cttgttggcg cacttttagg gaattttatt
     4741 actgatccga aaaagtttgg tttggatttc gccatcgtcg ctatgtttat cgggttactt
     4801 tatcttcaag tcatttctga tagaaacacc tctaaacgat tacagttgat cctgattggt
     4861 ctcactttga ttctggtata tgtcggactg atttttatcc caagtaactt agtgatttta
     4921 gttgtaacac tgattgggtg cggattgggg gtgtggatca aacatgcctt cttttgagtt
     4981 tacattgctg actattttag gttgtacttt agcaacttgg ctttctagga tcctaccatt
     5041 tgtcttactg aaaaaattcg atttgccgca accgttgctt gagtatttaa gttttgtccc
     5101 gatcgtcatt atgtctgctt tatggttcag cagtctattt acccaaaata tcggacatct
     5161 gccgcagatc aatctcgaaa atgctttggc ttcagttcct acacttttag ctgctattct
     5221 ttcaaaaagc ttattggtga tcgtcttggt aggaattctt tccctttcgt tgattcgctt
     5281 attataaata aaaaaacaag agcttatggc aaattactgt catgcgctct tgtttttatc
     5341 aaagtcaaat aagaaaaaaa tcggcgtatt cttgcttatc cgttgaacgg accactaaca
     5401 acgacttttc ctaatatatg tcctgtttct acgtctttgg ttgcttgttt caagttcttc
     5461 gcattcatcc catctgaata gaccttgctc aatgtactgc ggataatccc ttcatttgct
     5521 aaagcagcaa tgtgcgctaa agcttctcct tgcgtttcga tttcatagtt atagtctgtt
     5581 ttagcgaaca tatactccca gtcgaagctg atagattggt ttttccaaac acctatatct
     5641 aacggctctt tgatccctac gatcgttccc acatgtccaa atggtcggac aaggtgtttg
     5701 atttgatcaa agtagcgggt aatatcgaat aatacagcga catactctac ttgatcgatt
     5761 cctaattcac gaaaactttt acttaagtcg tgatgataat cgattggata gtctacacct
     5821 gtttttttca gccattcaaa attcttcgga cttgcagtcg cgtagacttc taggccagcc
     5881 caatgcgcta gttgattcaa aatcgaaccg acgcccccag ctccgttgat cactaagatt
     5941 tttttcccgc ggttggcatt ttcttctgga gtcagaccaa atttttcaaa taacaattca
     6001 taagcggtca atgaggtcaa cggcaaggct gcggcttctt catctgaaag attttttggt
     6061 gcaagagcga caatacgttc gtcaaccagt tggtattctt gattgcttcc tgctcttgtc
     6121 gttgtacctg cgtaaaaaac gcgatcgccg acagaaaatt tttgcacttc atcacctaca
     6181 gcaacaactt tcccgactcc atcaaaccct aagatacgta aagcatttct tatcccgtta
     6241 tcttggcgaa gtttcgtatc gacaggatta acagatactg cgttgacttt taccagaaga
     6301 tcccgtgaac tgggtacagg tatctgttct ttttcatcaa taaagctttt tggatcatcg
     6361 atcgacaacc cttcataaaa accgactacc ttcattaatt ccttttctga catacgaatt
     6421 cctccttagc ttcatcaagt tggagcatcc atacagtttt catttcaata tatatagtat
     6481 gctatttgtt ccacttttat tcatcataat tattctctta cttaaagtaa actatttgca
     6541 cttttttggt taaaaaagag gttgcgacaa gacttttgtc acaacctctt ttaaacggtg
     6601 ttcaatcaac tgaccctcgg tattcgctca aaaaacgaca aaatatgagg agctattttt
     6661 gtttggctgt tcttattact tgatgcagga cagctttttc acgacctcta attcacggtg
     6721 ttcaagcaag aaatcattgt tcactgaaaa gcgttggatg ttctttttca aaggtgtcta
     6781 agatctctcg tacttcatca gtagatttcg tattcatcaa ctgaactcgc aagtcatttg
     6841 ctcctggaaa tcctttgata tagattttaa agaaacgatg tagtcctgtg atggaacgcg
     6901 ggatgatttc tgaataatga tcttgcagat ctagttgcag acgaagtagt ccgatcagtt
     6961 ctttttctgt atgttccctc ggttcttttt caaatgcata aggattcttg aaaatccccc
     7021 ggccaatcat gatgccatct acgccatatt tttcggccaa ttcttctcct gtctggcgat
     7081 ctgggatatc tccgttgatc gtgatcagtg tttgcggagc gatttggtca cgtaaagcaa
     7141 tgattttagg gatgacttcc caatgtgcat ctactttact catctcttta cgcgtacgca
     7201 aatggacaga aagattggct atatcttgat gaagtaggtg actgatccat tcttccatct
     7261 cagacatttc cgtatagccg atacgcgtct tgacgctgac aggcaaaccg ccagctttag
     7321 ccgcttcaat caattgtgcc gctacttctg ggcgaaggat caagccactg ccttttccac
     7381 gttctgctac attagggact gggcagccca tgttgatatc gattccttgg aagcccattt
     7441 ctgccatagc aatgctcatt tctctaaaaa attctggctt gtctccccaa atatgggcaa
     7501 ccatcggctg ttcatcttct gtaaaggtca atcttcctcg tacactgtct tttccatctg
     7561 gatgacagaa actgtctgag tttgtaaact cagtaaagaa gacatccggt gctgctgcat
     7621 gctttacgac atggcgaaat acaacatctg ttacatcttc cattggtgct aaaacaaaaa
     7681 aaggctttgg cagctctttc caaaaattag tagtcattta tttgttactc tccattctat
     7741 caagcagtga agtcgcaatc tttctcaaaa acattgtcat tatactagga aacaaaaaaa
     7801 gaagcaaagt gaacagctta aaagatgttt ctgattgagc aaaaaaagac tgtgacaaaa
     7861 atcttatcac aattcctttt ccggaataaa tggtgttcaa aagcttgcat ctgcggtaat
     7921 aagtccaacc aaacgaaaaa taggaggagc tatttttgtt tggctgttct tattacttga
     7981 tgcagaacag ctttttcaca acctctccac taaacggtgt tcaatcagct gaccctcggt
     8041 attagctcaa caaacgacaa aatatgagga actattttcc cgtttgtttc tctaatactc
     8101 aggtcaaaac gctgatttca caacctcttt tctaacttct caatcctctt cctttattga
     8161 tcaaatacca agtaacggcg tacaaaacaa cgatgaatag cacaagtacg atcatcgatg
     8221 ttgctactgg cacatcgatt gttccaagga atccgtaacg gaaaccggag atcatataaa
     8281 cgatcggatt gactttcgaa acagcttgcc aaaatggcgg caacatagag atggcataga
     8341 aaacgccacc tagataagtt aacggctgca agacaaatgt aggcacgatc gatacatcgt
     8401 cataagactg agcaaaaatc ccgttgagca gtcctgctaa cgaaaacaag attgccgtca
     8461 tcaataatgt aatgatgacc atgaaccatg aatacacatg aagtggtaca aagaacaagg
     8521 aaatgatcgt tacgagagag ccgacgagaa tactgcgacc cactccgcca atcacaaagc
     8581 cccaaatgat cacatgcgta ggaacaggcg ctaccaagat ttcttcgatg tttttctgga
     8641 atttttgaga gaagaaggac gaagaaacgt tggcatacga actagtgata gcagacatca
     8701 taatcaaacc aggcacgatg aattccatat atgaaaaacc acccatgtca ccgatccgac
     8761 caccgatcat ttttccgaaa ataacaaagt acaaggatgt cgtgatcaca ggcggaacca
     8821 gtgtttggac ccagatacgc aaatagcggt tcgtttcctt gatagctaaa ctttttaatg
     8881 cagtaaaata aagattaaac atttttctcc tccgtatgat tattttcttc tgtgattttc
     8941 aaaaacagtt cttctaaacg attcgactta ttcctcatag aaagaacttt gattccttgt
     9001 cttgtcagct gatcgaagat tccgttcacc ccttgatttc gttcaacttc gacagcgatt
     9061 gtctgttcat cttcaaatgt acaagcgtat ccttcaatca caggtttggt ctcatatggc
     9121 gctaaatcaa agataaatgt ttcaaattgc agtttggata gaagctcttt catactagta
     9181 ttctcgatca gttcacctga ttggatgatc ccgatgttgc gacaaagcat ttcagcttct
     9241 tctaaataat gcgtcgtcaa aatgatcgtt gtaccatttt cgttcaattc tcggagaaac
     9301 tcccacatct cgcgtctcaa ttcaatatcg actcctgctg ttggttcatc caagatcagc
     9361 aaacgtggtt catgcatcaa tgccctagca atcatcagtc ttcgcttcat tcccccggaa
     9421 agcatacgtg cacggacatt tctcttttcc caaagattgg actgcttcaa atatttttcg
     9481 ctgcgtttca tcgcttcttt gcgagagaca ccgtagtagc ctgcttgatt cacgacgatt
     9541 tgctggaccg tttcaaaggg attgaaattg aactcttgcg ggactaatcc aatctgttgc
     9601 tttgctcgga ctagatccgt atccagatca taaccgaaga cattcacttt tccagatgtt
     9661 ttattgacta aagaagtaat gattccgatc gttgtagact tcccagctcc atttggtcct
     9721 aatagcgcat agaaatctcc ttctttcacg gaaagatcga ttcctcgcaa tgcttcgacc
     9781 ccacttccat aaaccttttt caaattattt atttcaagcg catttgccat ttatttccta
     9841 tctccttata gacatgattc tttattgtat catggaatac agcagatgaa aaaacaactt
     9901 ggttccagcg aaaaatcagg ttgctgggct caggaaaatc tcctaatgaa agacatcaat
     9961 cggacgaagt cagacaacct tttcagaaaa aacaaatcga agataaagga ttactattca
    10021 tatgtgcaac aaaaggagcg tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa
    10081 cacgtataaa cggcggaagc aaaagccaag atttttgatc caataaatac tttcgatcac
    10141 ttcgcctatc ccaatacatg ccactgccgc ttttcatcaa ataacgcggc ttccgttttt
    10201 gcggtttcta ctaaagtctc tgggtaatcc aaaactttca gcaaggcaat cgcatttcgg
    10261 gtccttgaag gtccttcttt taatttgaaa tcaaaactga ttccttttcc ttctgttact
    10321 tgttcttcaa agtggacatt ctcacaataa ttctttaaaa tctctgtcaa ttcgatatca
    10381 tgtgtggcga caaatgctaa agatggatac tccgcaagcc accgaacgac tgaagcggaa
    10441 gaagcaatcc gttcgatcgt atttgtccct tttaaaatct catctacgaa gcagtagcag
    10501 cgttctttgg aagccacttg gtccaacaaa cgtttgatcg atttgatttc tgaaacaaaa
    10561 taactgtcgc cttcaaataa atcatcttcg attgccatgg aagtcaacac atgtccaggt
    10621 tgcagggtaa actgttctgc aatcgctgtt tggatcgtct gtgctagaat acagctgatg
    10681 gctatacttt tgacatacgt agattttcct gatgcattgg aacccgtgac taatgtattc
    10741 ttctgccagt tgaccggatt cactaccgct tcttttaaca atggatgata gatatctttc
    10801 gctgtcacac cgccctcagt aaactctggt cggcaagtga tgggcatata agtacggtaa
    10861 ttcaatattg cagcggctac ttctaattct cccagcaatt cccacagctt gatggcttca
    10921 gttgaatgct tttctaattt agtgagaacg aaatgatagg agataaatgg cagcatgaac
    10981 atgatgttga catattcaaa aatcaactct gcttcactcc cactcttcat acgaaatgaa
    11041 aaacctaatt tagggatgtc tcttaatggt ttagtttgct gtttcagctc ttcttgaagt
    11101 ggtgtcggca gttttgctag ctgattggcg gctgaaatcg ttgaaaccaa ataactcatg
    11161 ctattcagct ctgtttctag tgtttgtttc ttcactagat aatagaccgt attgaatact
    11221 aacccaccga ttgccaaata gattcctagt acttgtccta aaagaagcaa acccaaaccc
    11281 accagcggaa gcaagccaag aaaaataaat aaacctacgc gtccaatctt tttacttttt
    11341 ccatccgcta gatattgttt tgcaaaatta tgatcttttt ttcctaaacg agcgaattga
    11401 tattggattt tttctctcag ttgaggattt tcctgataaa aagcaatcag tttttctaac
    11461 tgctgatctt caccaaaatc aaagctgcgt aaacgttgat acaacgcttc tgaacctaca
    11521 ctagaatagg tactgttgat tccttcgaat acttcgaaca tatccaggtc ataccaagta
    11581 agatcatcga tttcgctatc ccatgaacgg aacttcttct ccgtgagcca tgcttccttc
    11641 aagctttctt ctttatcaaa tctcggttga taaggaattt tttcccattt gctacgaact
    11701 gtttccttta atttcaaacg tgaaaaaatg tctaaagcaa ctaataaaat aacagctgca
    11761 ataataatta aaacgataaa ttctctttga cccatatccc tctccgttcc tttatttgtt
    11821 agctttagta tagcactgtt gcgctatttg aaaagagggt aaaaaaacac ctttcaaaat
    11881 aaaaggtgtt tttttcttgt atattacata tttatctctc atttatcaaa attgacaaat
    11941 aatcataggg aggaattgtt gttaattctt gtttattctc cagtgcattg acaactgtcc
    12001 caagggaaag gatagcctca ggtactattt tttcttgttc tgttggatta gctaagcgaa
    12061 gaacaaatcc ttgttcatga aaataagaag gataaatcga tgatacaatt aagtcttcag
    12121 gtaattcaat caatgaaaat ttacgaggaa catcaagttc attttgtaat ggccaaatct
    12181 tgttatctaa cctgtgaata aatacattca gtttctgctt ttgataagaa atagattgtt
    12241 ctaaccgttt ttctgcctgt tttgccactt tgaactcatt aaatcttcct tcttctacat
    12301 taaatccaaa agtgacttta tactcaccta tctcttgagc tagcggtgtt tccatcatga
    12361 catgcccttc atttgtcgta tctcctgaag ctcgcccagg tcgccaagca agattaggtt
    12421 ttccaagttc ccctgttgtt gaaaataagg ttatgattaa ttgattttct atacgttcat
    12481 actcttttaa accatctgca taaaacgtaa aataatgatc attttctgcc acagagacac
    12541 tcttatcgaa tatttctaaa ttaacaggtt tctctaccat agtttctgac caattcggag
    12601 gaatgacatc aggggtattt tcaataaatc cgttttgaat ttgtgcaatt gatttttcag
    12661 atttatctaa aacattaatt gctaagcgca tacgatgcga gtaaacagta ttatgaattt
    12721 tgagtgtgcc ttcaacgatt tgactttctc ccagactgaa cgatatttca taagatattt
    12781 cttccatttc gggtttttca gataaacgat cttttaaatc tttaggaagt tgagcttttc
    12841 cgtaaacaac taatgtttct tgtaaagaat ctttatatac tttagctgtt tctaatttta
    12901 gagatagttc tgtatctcct tctaatggtg aataatcata cgtatcgcca tcatttgctg
    12961 aatcgatcaa atgaacgaaa tcaagatatt gtctatctcc aactatcaaa ttcaattgcc
    13021 catctttaaa agaaagttta tatttattgt tacttatctc agttttgtct atctcgattg
    13081 ctgtttctaa tttttcatta tttttctcaa attcaacaac agtgtatcct agtgctggca
    13141 catttacatt tatttttatg tcaagttcat aataagctgg ttcatcaaaa tattcgaaac
    13201 ctgtagcaat catccgataa gcatttttcc gttctggaaa ataaacttct ttttcaatga
    13261 tattataggg actacctttg aagtgtattt tctttgaatc agctactaca tgcacgattt
    13321 tcgatccact ataattctca gtcgttgtgt tgaaaacaag gacttcattc gattttagct
    13381 ttaatgcatc agccatccgt tttacaataa ggttttcgat tccgtcagct atctcattag
    13441 cttctttgat tcgatggaaa atatcttccg caacattatc tgaaacacag ccaccaatac
    13501 tatcatgagc ctggttttgc aaaatctttt tccataaccg acgaagtagg cctttactga
    13561 tatggatacc atttctttct gcaatcagca tgagcggctc aactcgtttt agaatttttt
    13621 gttccaattt aaaattttct attttcattt ttgttcttac tgagccaata gaacgatgaa
    13681 cacgagagta aaccggctga cgaagttcac ctttatattc aggtaattct tttttgtttc
    13741 tgataacttc tacataagaa ggataatctg aaataatatt gtggaattta ctgtatttat
    13801 tcaacttctc tacgacttga tcaaaattta aaatcattga cttttgatct acacccgaag
    13861 gtactaaaat atctttgttt tttccttgca tatgaagcat ttcaactgct tcatccaact
    13921 tttcttttgc aaattcagga atatctttaa ttgcgtctaa ggtcatcatt ccagtcatat
    13981 aaccaaatgg aaaattcatg gcataaacaa atttatctcc caatcctttc cagataaaat
    14041 aaggagatgg aaccatcgtt tcaaagttta atcctcgcca acacatgaaa ttatcgattt
    14101 ctacttgatg caaaagtgta ggcaaattcg cattgaaccc aaacgtgtca ggcaaatacc
    14161 ctaccatcat tggatctcca tatttaagga tcgtatcact gattccaatc attaagtttc
    14221 ttaaaattga ttcagcatcc actaataagg catctgtttg tgcataccac ggtcccacga
    14281 atagttgtcc tgttttcatc agtcgttgga taaccttaag cttttgcgga ttgatctcca
    14341 aatactcatc gacgattgaa gtttgtccat ctaaacaaaa gtttacttta gggttccttt
    14401 ccaattcatc tagaacttca gtaaagacct gatcagacaa tacgatggca tcttcatttg
    14461 taaaatacca ttctctgtcc cagtgcgtgt gttgaattat atgtgcattt accatatcag
    14521 attctcctac tttccttctt ctactgaatt tacttgttgt tcttttttat acttgatgga
    14581 ttttagtgca ttgaccatta acgctgtaac cactccccca ataacaattg atactaaata
    14641 taaaggtata ttcgtaacaa aaggaattgc cataaaggtt gaaagcattg tttcttgagt
    14701 aacaccaaaa aatccattta gaccacatgc tactgcagtg cccaccacga tgcttggtat
    14761 tactcgtaaa ggatctttca cggcaaacgg gatcgtccct tcagtaatcc ccacaaggcc
    14821 cataatcata gctgtctttg cattatctcg ttccgattca tcataaagtt ttttgtttaa
    14881 aattgttgct aaaaacattc caaatggcgg aatagcaatt cctaataaca tggctgtact
    14941 aggtccaaag cttccgcttg aaaaagaagc aaccccaaaa gcatatgcga ttttattgat
    15001 aggtcctccc atatcaaacg ccatcattcc tcctaaaata gccgacatca aagcagcatt
    15061 tgctcctgac atgccttcta gccaattagt cagtcctgtt gtcattgctt gaataggcgt
    15121 agctagtaca taccataata tgattccaac aactaatgta gtaacaaaag gaatcagcat
    15181 aattgatttt agactaataa cttgcatcgg tacttttact tttttcaatg cattagctaa
    15241 atagccacaa gctaatccag caaccattcc tccaataaat cccataccaa gattttgaca
    15301 catcatccca gtgataaaag ctggtgctag accgggacgg tcagcaattg aatatgcgat
    15361 ataggctgaa atcatcggta cgataaactg taatcccgtc tgaccaatca ttcgcaggac
    15421 tccccagaat gtatccgtct ggtcccaaac acctgtgcca ccaaaaatga ctgcaataga
    15481 agttagtaac cctgctgcta cgacaagcgg gatcatatag gaaaccccac tttggaaatg
    15541 atttagaatt tctcttcctt gtttcttgaa ttccattagt aactccccca cgatatattt
    15601 tttatttgtt aattaatgca attgcttcat caatcgtctc ttcaccatct tttactacct
    15661 ctgaggtccc tactgttaaa atcggtttac cgtcaaagcg acctcgttct gaaacatttg
    15721 tatctactgc aaatatgaca acatctgcct cggaaacatc cgaaattgaa agtttgtttt
    15781 ctatccccat agccccctgt gtttctactt taatatctaa ccctttcttc ttcgcaaact
    15841 tttctaaatt agcttttgcc atataagtat gggcaatccc tgcgatacat gctgttactg
    15901 ctactatttt tgtcatatga aattcctcct tttaaaatga tagagctttt accaattctt
    15961 ttttatttct cgaatctttt attcttttaa caaactcgtc atccatcaat cttccagata
    16021 attctgctaa aatacgtaaa tgattagtac ctttatcagt gtcttttatt gctaacaaaa
    16081 agacgagatc aactggttga tcatctaatg cgccccattc aacagaccgc tttaacttgg
    16141 ctacagccac tgttgattca attactgcta ggcttttgcc atgaggaatg gcgatatttt
    16201 tcccaattcc tgtggtcata tggttttctc tgtcaaatac gtctttttga aattgtttgt
    16261 ggtcagacac aaacccatta gtctctaaca atttcgataa tttttcgact acactttttt
    16321 tatcagatgc atttaaatct atcactatat tatttatatt gattttttca ctgatcattt
    16381 ataagccttc tttcaatata taaactaatt tttcaaacga cgaactcgtt tttaaatttg
    16441 ctaataactc atgattcgaa gcgatttcat tgaatgtttc ataaatttta ttcaattcag
    16501 gttttacttt ttcattaagt gcaaaaaaga aaacagaatg aacaagtgtc ccatcccaat
    16561 caattccttc tggattaacg tagacacaga ttactggctt ttttataaaa tctacttcga
    16621 tatgcggcat agcaaaatca ttcatcgctg ttgaagatag tttttctctt ttgattgctc
    16681 cctctagtat cccttctttc gcgtaatttt cttttaccaa ttttttggta attatattta
    16741 aaacgttcac ttgattatcc tttatttggt gggtaaaaaa gcaaaatcgt ttatctaata
    16801 aatttagaaa agcatcttct gtttgttcat ttaactgtag atactgtttt acttttctga
    16861 catctgcccc taacatcaaa ggactaacaa caataacagg aaaatttgta ttttcaatcg
    16921 gaatcgtgct tataacaagt tcatcagtga cttttctatc ttgtagttca ttgatgctta
    16981 gtacatcttc tatctcaacc atatctgcaa aaccgttatt tattctttgc tctaataatt
    17041 ttgatgttcc atatccgcta ctacaaacta atatcacttt cgttttgtca ggtttgtaac
    17101 gatccaaaag tgattgaata tggagtgtta cgtaagcgat ttcatcttca ttaaaatgta
    17161 tacagaatct ctcttcgatt tcctctaata aatctacact tatcataaat gaccttttaa
    17221 aagaatattt gattttgtcc gtataagggt tatagatatt tactcccaat ttgagtcttt
    17281 taactgctgc attgagatgt aatactagat tattgatcaa ttcttcatca ggataaaatt
    17341 catttaattt aattcttact aactcttgta gctcaacacc catatctaaa taggaaagct
    17401 tcgtttgatc taagtcgtga ttcatagcat ttttttcaat cgaagtaatg tgaatgtcaa
    17461 cataagcttg ttcatctatt ggcacaataa ttgaaaatgc tttttcaatc attttgatta
    17521 aaataagtgt attctttgag atttcttctt cagaattatt actacgtgat aagaaattgc
    17581 ttgtttcaat cctttctatc gcaatagcaa ggtggatagc taaagtttga aattcataat
    17641 ctgtaaattt caattgtgtc ttattcatgt atcgattaag caattcggct acttttttca
    17701 atatgtcctt atttaagaaa ttattcgctt caccaatgaa attgatattc aagtctaaga
    17761 gatctgtttt actgttagat gctgtaaagc caccccaata atagttcaca acttctgaca
    17821 tcaaccttct tttacttttt tcatcagctt tgatgcgcac gccatttcga tctgaaacta
    17881 aggacatccc atgcttgttt agtctttttc taacatcttt cattgtgctt tctaccgtcg
    17941 acctgctcac gtataattcg tcagcaagat tttgaatctt tagagaatca ttagtattca
    18001 ataatttagc aaacacataa gttagacggt cctccggaga gtctgcgata ttgattttat
    18061 gactggataa taaatttcga agtgtttctt tttttccaat aagcgaaacg ccttttttag
    18121 gttgaaccaa catttgtaca tctggcattt tttggattat ttctttcaaa attaaaatat
    18181 ctttgctaat tgttttcgta gatacatata attgttctga taattctttg taagttgtgt
    18241 gctcattttc aatcaaaaag tatagtattt tcatttgccg ttcgctaaat tcgatgccca
    18301 ttatcttcac ctcctgaata cagcttaagt gtacaattat tatctctatt ttcatacaga
    18361 agaaagttct tgattaatgt agaagtttct aatattcttg attatttcaa atcaataaaa
    18421 aagcctgcaa tatttatatc acaggctttt ttctctatta aatatatata agataataca
    18481 tttttctact agttctcact aatcttgtat aattaaaccc tccgcaactt gacggagatg
    18541 gagaagatgc ccccgtctaa tctagcggtc atctctcctc ccattccttc caccaatttt
    18601 tttgacaggt aaagcccgat tccgctagat tccgtatttg tgcggctggt attttcggta
    18661 taaaaacgct ggaagacttt ttcaacagag gtttgcgttc ctggtttgat aggattggcc
    18721 actgttaatc gaatgaatgg atcctctttt gaaaatgtca cctttgcttg tccatctgca
    18781 taacgcaata aattctgtat gatattttgc agcactcgca gcaacatgag cggatctgct
    18841 aatacagttg tatttatcga gtgttcagcg aacgctacct cgatattttt ctcagaaaat
    18901 tgttcataaa aagagaaaaa tacttcttcc acctctttag aaaaagaaag cgtatggaga
    18961 tccatttctt tttgcttcgt ctctattcga gcaagatcat aaaactgatc cgtcatactg
    19021 agtaatcttg caacagattg tttgatgatc gttaaatact tctcccgttt ctccgtatcc
    19081 tctacctgtt ccagtaattg tacataaccg ttaatagcag tcaaaggagt tctgagatca
    19141 tgagaaaggc cgacgatcga tagtctgatg ttttcttcca tttcttgaaa tgcttgtcct
    19201 ttttgttcat tttcatccaa ccatgtattc agtgcatcta ctaggtccat tagattcttt
    19261 tctcgaaatt cgactgaaag acgaccacca tattcttgtc gttcagaaag agtggcgatt
    19321 ttcttagtta atctcttgat ttccctttgc aggaagaaaa taaaacttcc cataaagata
    19381 acaacgagaa gcaacagcca tttaatcaaa tctaaacccc actccccaca ccgtatgaat
    19441 atacacagca tgtgcctttg ctagcttccc tcgaagattg gaaatatgca tattcactgt
    19501 tttctcacta tcaaaatatg gttcttgcca aactgtttca taaagatttg ctcgcgaaaa
    19561 tacttttttc ggatgttcta aaaaaagctt cagcagatcg aattctcttc ctgtcaaatg
    19621 gacagccaca ccattcacat ttacatccct cgactctttg tccagtaaga tttccttgta
    19681 atagatgcgt tgcgacagat cttttacttg gacagaagtc tgatgacgaa gttggatgcc
    19741 gattcgtaac aaaagttctt cgatatcaaa tggtttcgtc acatagtcat ccgctcctgc
    19801 tgccaacaag gaaactttat ctctttgatc gctttttgct gaaagaacga tgatcggaac
    19861 attcgatgtc tgtcggattt ctgctaacaa tgcttcacct gaaagccctg gaagcatgag
    19921 atctagaagg atcaaatcga caggatatgt gcttaacaat aattttcctt ccgtacctga
    19981 ataagcatca agaagtttgt atctctgttc aagaacctcc tccagtaagg aatgaacagc
    20041 ttcatcgtct tcgataatca aaatagtttc cagcaggacc acctacttaa tgtcagattt
    20101 ttggaaaata aacattccta gagctgatga taccacgatt gttactagac tgaccagaat
    20161 cattttcggt gcttgcgaat aagttgcttg ttgcgcaaag tccgtataca tgagccacgt
    20221 ttccgtaaac cactccgtta gaaattccaa gcctttgatt tttgcgaaaa caccgggaat
    20281 gaaggccgtt cccatgatat aaagaaaaga aagaagcaaa ctgacactgg tacttctagt
    20341 gatgaatgtc aaagccactg gaacagataa tgtccctaat gtcagcagaa gcaatacagg
    20401 aaatactttt agaacataat ttccatagtt tttgattccc tcaacttcac caaatccgaa
    20461 agcaagtgtg taagcaagca tggctagtcc cgtgatcaaa accacgccaa gtgttgtgat
    20521 caaaagcgta acgagaagtt tcgacacata ataagtcatt cgattccgtc ctaaagataa
    20581 cgtattgcga atcgtaccgt tttgaaattc ttccccaata taaaagcacg caaatgctgc
    20641 gaaagccaga agaatccatg cagccatttt ttctaccatt aaaatccctg ttgcaccgtt
    20701 gatgactgta tccgttgctt ggtttaattc attgccgtat tttgtatgca tataccattc
    20761 gttccaagaa taaaaaagcg gaagtacgac catcagtcct agtgagaccc acattttacg
    20821 gctcttccat acttttattt tttcagcagt taatagatta aacatgtgga ttgcctccag
    20881 ttaattgtaa gaaataatct tccagttttt gcccaactag gtgaatacca tccacaggaa
    20941 cttttgcttc taccaatgct tgattgatgg ctgctacttg gtcaattcct tcaaaaagct
    21001 ggataacacg gctagattgt acaaaataat ccctataacc aagagagtcg agaaccgtta
    21061 ctgcttgttc cgtcgccgaa gtgtcaattt ttataaattg acgagattct tgtgccaatt
    21121 cttctttcga taattctttg atcaagtgtc cttcatgcaa aataccatag tgagtggcga
    21181 tctgtgacaa ttcatctagc aagtggctag aaagcaagac ggtgatgccc cgttctttta
    21241 ccaaacgttg gatgatttct cgcatttctg ccatccctgc tggatccatc ccattcgtcg
    21301 gttcatccaa aatcaaaaat tctggattcg tgatcagtgc atttgcaata gctaaacgct
    21361 ggcgcatacc taaagagaaa ttctttgctt tctttttccc tgtattttcc aatcgcatca
    21421 ttttcagcag gtcttcgatt tctctatccg acacaccgcc gttcgctgct tggatacgta
    21481 ggttatctct tgcagttaat tctggataaa gagcaggggt ttcaatcgat tgacccatac
    21541 gtcgtcttgc tgcctgcaaa tccgtcgttc caaataattc gatcgaacct tcatcaatcg
    21601 agatcaaacc cataatagtt ctcataaagg tagacttacc cgctccattt tccccaatca
    21661 atccatagat cattccacgt ttgatttcga ttgatacttt atctaatgct ttggcagcac
    21721 cgtatttttt ggttatacca gttgctttca agaccgtttc atttccaaaa tccatactta
    21781 tccttctttc ttattgatga gtgaagtata gttgacgggt ataaagaaag cagccagaaa
    21841 tgtataaaga aagtgtaaat tttccaattg aatgtcaaaa aaaacaatcg gacaatacgt
    21901 gtctttccta ttatcaggca gtttgttata cgcttgtatc agtttcctga ttctttcttt
    21961 tcgaccaaag tcgtttctct gtattcctct tcactgaaaa tttttgcttc tttcagatca
    22021 agcttgagta attttaccag accggttata aggataacta tagcaagact ccctcccaga
    22081 aaaattagac tgtctcgcag acgtggttgc gttgtcttcc aatcaaagag cgcaccaaac
    22141 agtgctaatc caagtggact ggccaccatg gtaatcgttg ttatcgttga cataagtcgt
    22201 ccttgtatat gcgttggaac ttctcgctga agccagacca tataaaaagc aaaagaaaac
    22261 attaatcctt aaaaacggac ttatgaaaaa taatacatgc cgtttgggat ttacaattag
    22321 ctaccaaata agaggttatg acaaaagact tttcacaatc ccttcttccg aataaacggt
    22381 gttctcctgc caactcctcg gtcacaagtc acaatattta tcaaacatga gaagcaattt
    22441 ttgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc agaagcggat
    22501 aaataatatt gtttgatgat actttatctt tttcccgttt gtttatagat cgaatgatat
    22561 tatagaccaa gaatcctacc tattaacata gctaaaaaca caaaaaaggc actttccaaa
    22621 ttgtggaaag tgccagaaaa gttaggatta aggtaactgc gctcagcatt gcttcgccca
    22681 gtacgattct cgtaactcgt gaactcgaac gataatcgca attaacgttt tgagaactgt
    22741 gaagctttac gggctttctt aagacctggt tttttcttcg tatcattgaa taagtttgaa
    22801 aaaccttgat ttataaaggt tttcccgtaa atatatttta ttatatttga ttaaatttga
    22861 aatgtttgtg ttctaaaatg tgttctaatt taaaggcagt atatttcgta ttttagatta
    22921 aatttttagg ctcattcatt ctcaaatcca ttctattcag tcaataaaaa ttttaaaaag
    22981 actttataaa caaagatgtt caagcgagca tcttattttt ttgtgcttaa tttgaaagga
    23041 gaacatttca tgtataaaaa acaaaaaaag aaattatgtg tattaactat tttgagtttg
    23101 gctgttcaag caattgttgg agcaattgct cctacgatta cttttgctga tgaaattacc
    23161 catccacaaa cggtaaccgt agaactagat ttagcacatc aatatgctgt tgaaggaact
    23221 tttagtgatg gtcgtccaat gtcagaagtc actgttccgc attatgcagt gtacaacggc
    23281 tttaaacaag atattttctg tattgaacca ggagtcccga tttacaatga gttcactcct
    23341 ggctatgaga aaaacccgtt accagatatg tccgataaag ccaaattagt ttccgtttta
    23401 tggaaaaaag taggtacaga tgcagatact catattgtgg cacagaagat gatctggcaa
    23461 gaagttaatg ggtataccct tcactcaata aaacgatcag atggtagtgc cgtaaatatc
    23521 gcagccattg aggcaaaaat aaatcaagcc attgacgatt atcagaagaa accaaccttc
    23581 cataatagta cggctaaaac agtattaggg caatcgacta ctgtgacgga tacgaataat
    23641 ctgaatttgt cagagttcga tgaggttgtg gaaaatgccg caaacattga ttatcgtgtg
    23701 aatggtaatc agttgataat caccccaaat gccaattcaa aagaaagtgg tgtgctgacg
    23761 cttaagaaat ctgctggtac cggaacacct gtcgcctata aaaaggctgg ccaacagact
    23821 ttaatggccg gggcgattga taaacctaat acctacacaa tcaaaattga tgtggaaacg
    23881 gagggttctt taaagatcaa aaaagtcgat aaagaatcgg gtgatgttgt accaggaacg
    23941 gttttccatt tagactttgg aaaaacttta cctgcaaaag acgtgactac tgacaaagaa
    24001 ggcattgcta cattggatga gattcctcat ggtacaaaag taaccattac cgaaaaatca
    24061 gtaccggcgc cttatacgat tgataccacc cccatgacta ccaccattaa agcaggcgaa
    24121 accatttacg tgacttcaaa aaacgcacgg gaaaaagggc aaatcattct ggataaaagc
    24181 ggagttgaaa cagggagtga tctttggaat gacaattatt ccttagctgg taatacgttt
    24241 gccattcgca aagacagtcc aaccggtgaa atcgttcaag aaatgaccac ggatgaaaac
    24301 ggtcatgcgg aaacaccaaa agagattgct aatgcgttgg aattgggaac ctattatgtg
    24361 accgaaacta aagctagtca tgggttcgtg aatacgttca aacctgtaaa agtcgaattg
    24421 aagtatgcca atcaaaccgt agcccctgtt accagtaacg taaaagggca aaatcaagaa
    24481 gtcactggcg aaaccacttt gacgaaagaa gacaaagata ctggggataa agcacaaggt
    24541 aaggcagtct ttgagggtgc tgaatacacc ttgtttactg ctaaggatgg aaaagctgtt
    24601 aaatggagtg aggcttttaa accagagtta gcgaagggaa cgaaaggttc tgatgaaacg
    24661 gtcactttga ctttagacga aaagaaccaa gttgccgtta aacatttagc tatcaacgag
    24721 tattattggc aagaaacaaa agcgcccgaa ggatatagcc ttgatgaaac aaagtatcct
    24781 gtatccatca aaaaggtgga tgatagcgaa aaaaatgccg tgattacccg aaatgttacg
    24841 gcaaaagaac aagtgattcg ctttggcttt gatttcttta aatttgctgg atcagctgcg
    24901 ggcactaccg aaactggctt caatgatctg acctttaaag tgtcgccatt ggaagggacc
    24961 aatgaaatca caggtgccga agatgaagcg accacagctt ataacgagca attagggttt
    25021 gatggttatg gcaagtttga aaatcttcct tatggggatt atttacttga agaagtagaa
    25081 gccccagaag gttttcaaaa gattacgcca ttagaaatcc gttctacttt taaggaaaac
    25141 aaagaagact ttgtgaagag tgagtatgtc tttaccatta ccgaacaaga ccaaaaacaa
    25201 ccaattaaaa cggtgaccgt tccttacgag aagctgacga acaaagcatt ttctgttagt
    25261 ttgaaccgtt taatgctcta tgatttgcct gaggaagaag atagtttaac ttctcttgcg
    25321 acttggaaag acggaaacaa agaattaact tctctggatt ctaccgagct agttgataaa
    25381 ttgagttaca atctacatga aatcaaagaa gactggtatg tggtagcgca agccattgat
    25441 gtggatgcga caaaagctgc ccaagaaaaa gatgaaaaag ccaaaccagt ggtgattgca
    25501 gaaacaagcg caacgttggc gaacaaagag aaaacaggaa cttggaaaat tcagcataaa
    25561 ttaaccgccg aacaagtttt aaataaaact attgtcttat tcaactatgt gtatgaaaat
    25621 aaggaagctt ttgaagcagg cgataagcca gtagcgaagg atgttagctt gaataaccaa
    25681 gcccaaactg tcagttgtac agtggaacac catgtttcta tccaaacaaa ggcccaccta
    25741 gaaaacggtt ctcagacctt tactcatggt gatgtggtgg atatgtttga tgatgtgtcg
    25801 attactcatg atgtactgga tggctcaaaa gaagctttcg aaacaattct gtatgcttta
    25861 ttaccggatg gtacaaacaa agaaatttgg aaatctggca agattgatta tgaagtaaat
    25921 gataaagaat tcaccaaaac tgtgcttgcg aaaaaagtag ataccggaaa atatccagaa
    25981 ggaaccaagt ttacttttgc ggaaatcaat tacgataaag atgggaatat caatggaaaa
    26041 cacaatgaag atttgaaaga aaaatctcaa accttaacac caaaagaagt tccaaccata
    26101 ctaagtacgc cgaaacaacc ggaaacacca actgttccaa gtggctctca agaatctagt
    26161 cccacagtga agaccttccc tcaaaccggg gagaaaaatt ccaatgtttt actgttcatt
    26221 ggttttactt tgatttttgc gacggcgggc tattatttct ggaatcgccg gaactaaggt
    26281 gatgagatga tacaagtaaa tcctaagaac caacgtgcgc gaccgcctcc taaaaaagta
    26341 gtcgcgctat ttttgtatcc aaaactaaca atgaaaagag gaaaagaaga tggaattgaa
    26401 atttgtcgtg ccagatatgg ctgaaacctt tggaaagtta agttatgccg gtgaaggaga
    26461 aattataaca gaaggatatg gacggaatac tacggtgatt ggtcgcagtt atcatttgta
    26521 ttccagtaag caacgggccg atgatattga ggtggtagta gctgcggaag ccggtgaaaa
    26581 ggattttgat caagaccaac cgctaaaagc cgtgaatccc catttggttg ccaaaggcta
    26641 tgagattgag aatcgtgggt ttactgatta cgtgttgtat gttgatgatt tagtgaaagc
    26701 ataggaggaa aagaagatga gattaacaga aggtattgtc gtagattcag gtttaacatt
    26761 tgggaagttg cgtttttccg cattacgccg agaagtacgg aagcaaaatg aggatggaac
    26821 aattggtaat gaggtaaaag aacgaaccta taatttaaaa tcctctgcac aagggcgaat
    26881 gattcaagtt agtatcccag ctaatgtacc cttacgcgag tttgcttatg atgcagaggt
    26941 agagttggtc aatccaatcg tggataccgt tgccaattat gtttttcgag aaggaactac
    27001 cgttaattgg ttcattaagg cggatgattt agtcttgaag cggcagccga atcaaggaaa
    27061 tcctacgaac caaaatgaaa gaaagaaata ggtgaagatt atggaaatta atgttgaaca
    27121 tattttagat tgtctcgatc aatatggaaa aggcgagctg actgaggaac aactcgttaa
    27181 agcattaacc tctgatgaaa aaatgtttct gattatacat caaggtttac ttggagaaaa
    27241 tagcgacagt gaagaagact ctgatgtact agactggtta ggagaagaag agtctttctt
    27301 tatagttgtc gaagtaaatg aaaacttatg tcggcaggca gaatccattt tggaagaaat
    27361 tggtgtggag atgccggatg ctattgaagg ctttttaaat caattggttg aaacgaaaca
    27421 actcccctcc tcgtagcagt aaatgactag cataactttt tgaaattgcc taatgaaatc
    27481 ctattgggct ttttcgattg gaggtggaaa ccatcaaaat gtacaaagga catcgcatac
    27541 gcgcaggaga tcagcacttg gtttatcact ttgttcttgg ctggctactc gctctgttca
    27601 tcggttggat gagcgtcttt tattttcaag agtttagaca atttgatatt tctaagctat
    27661 cactttctac tattgaaatc gtttggtcta taaaagattt agtatgtctg cttggaagtc
    27721 tcgctttttc aggagctatg atattgcttt atattcactt ttttcttgat cattggcgaa
    27781 gcttgtggca tcgacaaaag ctagctcgga tgattctaga gaaccattgg tatgaagtga
    27841 aacaaactca aagtgaaggc tttttcaaag atttgaatag tagccgaacc agagagacta
    27901 tcagttactt ccccaaaatc tgttatcgaa tgaaagatgg attactgtct attcgcgttc
    27961 aaatttcact tgggaaatac caagatcagc ttttaaaatt ggaaaagaag ttagaaagcg
    28021 gactgtattg tgagttagta gaaaaggaac tcaaagactc ctatgtggag tatactttac
    28081 tgtatgatat gatcgccaat cgaattggga tagacgaagt agtggcagaa aatgggactc
    28141 tgcgattgat gaaaaatcaa gtatgggctt atgattcttt accccatatg ttaatcgctg
    28201 gtggcacggg tggagggaag acctatttcc tcctcaccat tatcgaagca ctattgaagt
    28261 cagatgcaga attgtttatc ctcgatccca aaaatgcaga tttagctgat ttaggtacgg
    28321 tgatgcctca tgtttattca caaaaggaag aaatttctgc ttgtgtggaa gatttctatg
    28381 aacgtatgat ggctcgtagc aaggcaatga aagaaatgtc taactacaaa acaggagaga
    28441 attatgccta tcttggactt ccacccaact ttttaatctt tgatgaatac gtggcttata
    28501 tggagatgtt aacgacgaaa gaaagtgcgg tgattttgaa taagctcaaa caaatcgtga
    28561 tgttgggtcg tcaatctggt ttctttctga ttttggcttg ccaaaggccg gatgcgaaat
    28621 atttaggaga cggaatccgc gatcaattca actttcgcgt ggctttgggt cgtatgagtg
    28681 aactcggata ttccatgatg tttggggagg tcgaaaaaaa cttctttatg aagcacatca
    28741 aaggtcgagg ttatgtggat acaggtggca gtgtgattag tgagttttat acgccacttg
    28801 taccaaaagg gtatgatttc ttagattcaa ttagtaaatt agaaaagatg aatgattgag
    28861 tgatcttcaa tactgacttt atacttcaag taacgtataa tacttttata tagtaggaag
    28921 aaaggggata attaatatgg aaagtaatga ttcttattta gagtatgcaa aagcaaataa
    28981 ggataagttt atttcttata ttatcaaagg gaaatccccc gaagttggac gagacgcagt
    29041 ttttatggca gggagtcctg gggctggaaa atcagaggtt gccattggat tagctgagaa
    29101 ttatgataac catgtgatta ttgatgcgga ttactttcgc acccagtttc ctgattataa
    29161 tggaaaaaac tctagtctct ttcaaaaagc atcttcttgg ttggtagagc aggcatttag
    29221 atatgtctta gatcatggct attcttttat tctggatgca acttttgcaa tattaagtgc
    29281 tgaaaaaaat atctcgcggg cgttgaaaaa acattttcga gtgacgattt tctatgttta
    29341 tcaagaccct aaaatcgctt ggaaatttac acaagaacgt gaaattgcag agggacgtca
    29401 tgtgccgaaa aaggcattta ttaacgcttt tttcaaatca agagagaaca ttgcaaaagt
    29461 aaagagtaaa catccagatg tattgcttca tatcataata aaagattata cgaataatat
    29521 ttccgaagtt cattatgcta cggataatct agaattattt ttaccactag ggtatagtag
    29581 gaaagatttg gaggttcaat tacatgactg agaaagaaca agtaacgaaa attgtaaaga
    29641 aatacaataa gagcattgcg gatttatcgg aaaatgctac tgcaaaagag ttcaaaacgg
    29701 tgattaaata tgtagccgat caggccaatg aaaaacaacg gaaattagtt ggattaaata
    29761 aaaaatagta ggacaaatta ataatgtatg agccaagagc aaaatgttct tggctttttt
    29821 ctttgcaatg aatgaggtaa tgtatgagat ttttagattt attcgccggt attggtggct
    29881 ttcgtttagg tatggaacag gcgagccatc attgtatcgg tttttgtgaa attgataaat
    29941 ttgctagacg tagttataaa gcaattcatg atacaagtaa ggaggtggaa atgcatgaca
    30001 tcacaagtgt atcagatgag tttattcaat ccctcggacc agtggacatc ctttgcggcg
    30061 gatttccgtg ccaagctttt tcaattgcgg gaaagcggca aagattttcc gatactcgag
    30121 gtactttatt ctttgaaatc gctcggttca ccactcttct ccgacctaag cttttattcc
    30181 ttgagaacgt ccgaggattg ctcaatcacg aaggaggggc tacgttcgag acgatcctcc
    30241 gaacgctgga tggattgggg tatgacgtgg agtggtaggt gcttaactca aaggcctaca
    30301 ttccccaaag ccgggagcgg atattcctta tcggacattc tcgagacgca tgtaccgaac
    30361 aagtatttcc tatcattgga ccgtcttcaa catctgatca aaacatcaga aatttggtaa
    30421 atatcaatcc ttccaatcga ggaatgggtg gacaagtcta tggttcagat ggcgtagcgc
    30481 caaccttaac aggtgatgaa ggaatcaaaa tcgcactgcc agcaagggat ggaatctctg
    30541 tagcaggact attaccggga aagtttgaac aaggtaatcg agtttatgaa gttactggaa
    30601 ccgcccccac cttgtcaacg aaacaaggtg gtacaaaaat catgattcga aaaaatgaaa
    30661 cagaatatca agaggtgaag ccagaagaca gtgtgaatct ggcttttcct aattccacaa
    30721 gtcgccgagg tcgtctggga aaacagagtg ttcatacctt actaacagga gaccaacaag
    30781 cgatcgtcac ggatcagtat caaattcgga agctaacacc tagagagtgt tggcgacttc
    30841 agggctttcc cgattgggct tttgatcgag attctcaagt caattcagat agtcagttgt
    30901 ataagcaagc cggaaactct gtaacagttc cggtcatttt tgatattgcc agaagattaa
    30961 aagagttgga tgtacatggt ttatgatgag ttgattggag aaatttattg ggtgattgga
    31021 aagattcagt cagacccaga attagaagaa caattgcgac ggttaaaatt tgatattcgc
    31081 aagaatggtg tcaaagtcgc tggcgatcca tatttaatga atgaggagac ggatgctcga
    31141 attgaaataa atcaagtaat tgcagaattt gagcggattg cggaccaagc aaaagaacca
    31201 gatattcgtc aatacttgtt agaaatgaaa gcagagttgg aagtaaatgg tattacagat
    31261 gagtaaccta aaccaacaca actgactgtt aggcgctgac cgcggaggat tcgcgaagca
    31321 gaaatcctcc gtggtgctgt gcctgttcat ggacggcgtt agccgaccat gatgtctcac
    31381 ccccgtatct aacagggggg tacaaaaaca caaaaaataa gtaagcaacc attaagaatg
    31441 cttgttgtat caaagatagt aagtaatttg tataggtgtt aactcatttg aaaaagttaa
    31501 caccttgaat ttggaggtga gaacttggca caacggaatt tagattatcg gctattaaaa
    31561 gaccgacgca acgaatacgg cgtttcacaa aataaattgg ctacggcttg tggacttagt
    31621 cggccgtact taaatcagat agaaaatggt ggcgtaaccg catctataaa aaccatgagg
    31681 aaaatttttg accaactgga aagctttaat cccgatttgc ctttgacgtt actgtttgac
    31741 tatgtaagga ttcgctttcc cacaacggat gcacgaaaaa ttattcaaga aattctccat
    31801 ttgaaatttg agtatatgct ccatgaagat tatgcctttt actcctatca agaacagtat
    31861 gtcatgggtg atattgtggt gatgttgtct catgaagaag ataaaggtgt gcttttagaa
    31921 ttaaaggggc gaggttgccg acaatttgaa acttttttac tcgctcaaaa gcgtagctgg
    31981 tacgactttt tcgaggattg tctaaaggcc ggtggcgtga tgaaacgctt ggacttggca
    32041 atcaatgatc gagtggggct attggatata cctgagctaa cgaaaaaatg tcagaaggaa
    32101 gaatgtatct ccttgtttcg tacctttaaa agttatcgtt ccggggaact tttaaaggct
    32161 gatgaaaagg acggcatggg caatacctta tatatcggca gtctcaagag tgaggtttat
    32221 ttctgtttgt atgaaaaaga ctatgaacaa tatatcaagt tagggattcc attagaccaa
    32281 actgagacga aaaatcgttt tgagattcga ctgaaaaatg accgtgcttt tcatgcgatt
    32341 caagatttgt taaaaggtcg tagcattgag agcaccacgt tttccattat caatcgttac
    32401 ttgcgatttg cggataaagt agaaggtaaa aggcgaacca actggccctt gaatgaacaa
    32461 tgggggcgtt ttattggacg gaatcggaaa gagattcaat taacttccga gccaaaaccg
    32521 tataccattg aacgaacctt gaattggcta ggccgacaag tcgcccctac gtggaaaatg
    32581 gctaaggagt tggatcgatt gaatcggaca acctatatcc aggatatggt acagaatgca
    32641 cgcttatcag accgacataa aaagattttg gagcaacaaa gtatggcaat tgaaaatttg
    32701 attatatgaa aagaggaatc gtcatgggaa ttgtaaaaga tatttttttg gtatttggtg
    32761 gcagtatgct tggtgttact gtgatgtgct tgatgaatgc aagtgcagca gcggatcgag
    32821 caatggaaaa gaaaggaaag tgacaagatg aatttcggac aaaattt
//
LOCUS       DACDMM010000013        32274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDMM010000013 DACDMM010000000
VERSION     DACDMM010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.013"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(302..967)
                     /locus_tag="IWD16_000472"
     CDS             complement(302..967)
                     /locus_tag="IWD16_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9087558.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            1093..3129
                     /locus_tag="IWD16_000473"
     CDS             1093..3129
                     /locus_tag="IWD16_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9087559.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            3133..3771
                     /locus_tag="IWD16_000474"
     CDS             3133..3771
                     /locus_tag="IWD16_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9087560.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            3799..4227
                     /locus_tag="IWD16_000475"
     CDS             3799..4227
                     /locus_tag="IWD16_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087561.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            4684..5595
                     /locus_tag="IWD16_000476"
     CDS             4684..5595
                     /locus_tag="IWD16_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9087562.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            5812..6249
                     /locus_tag="IWD16_000477"
     CDS             5812..6249
                     /locus_tag="IWD16_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087563.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            6344..7228
                     /locus_tag="IWD16_000478"
     CDS             6344..7228
                     /locus_tag="IWD16_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9087564.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            7452..10619
                     /locus_tag="IWD16_000479"
     CDS             7452..10619
                     /locus_tag="IWD16_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087565.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            10639..11184
                     /locus_tag="IWD16_000480"
     CDS             10639..11184
                     /locus_tag="IWD16_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP9087566.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            11212..11667
                     /locus_tag="IWD16_000481"
     CDS             11212..11667
                     /locus_tag="IWD16_000481"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP9087567.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            11664..12422
                     /locus_tag="IWD16_000482"
     CDS             11664..12422
                     /locus_tag="IWD16_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9087568.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(12508..13248)
                     /locus_tag="IWD16_000483"
     CDS             complement(12508..13248)
                     /locus_tag="IWD16_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9087569.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            13309..14562
                     /locus_tag="IWD16_000484"
     CDS             13309..14562
                     /locus_tag="IWD16_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9087570.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            14884..15111
                     /locus_tag="IWD16_000485"
     CDS             14884..15111
                     /locus_tag="IWD16_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9087571.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            15234..15560
                     /locus_tag="IWD16_000486"
     CDS             15234..15560
                     /locus_tag="IWD16_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9087572.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            15628..16341
                     /locus_tag="IWD16_000487"
     CDS             15628..16341
                     /locus_tag="IWD16_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9087573.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            16651..17502
                     /locus_tag="IWD16_000488"
     CDS             16651..17502
                     /locus_tag="IWD16_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9087574.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            17623..19488
                     /locus_tag="IWD16_000489"
     CDS             17623..19488
                     /locus_tag="IWD16_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9087575.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            19536..20945
                     /locus_tag="IWD16_000490"
     CDS             19536..20945
                     /locus_tag="IWD16_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP9087576.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            21053..21754
                     /locus_tag="IWD16_000491"
     CDS             21053..21754
                     /locus_tag="IWD16_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9087577.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            21913..22392
                     /locus_tag="IWD16_000492"
                     /pseudo
     CDS             21913..22392
                     /locus_tag="IWD16_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            23240..23815
                     /locus_tag="IWD16_000493"
     CDS             23240..23815
                     /locus_tag="IWD16_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP9087578.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            23815..24255
                     /locus_tag="IWD16_000494"
     CDS             23815..24255
                     /locus_tag="IWD16_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9087579.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            24271..24972
                     /locus_tag="IWD16_000495"
     CDS             24271..24972
                     /locus_tag="IWD16_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9087580.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(25460..25885)
                     /locus_tag="IWD16_000496"
     CDS             complement(25460..25885)
                     /locus_tag="IWD16_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087581.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            26259..27230
                     /locus_tag="IWD16_000497"
     CDS             26259..27230
                     /locus_tag="IWD16_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9087582.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            27262..28659
                     /locus_tag="IWD16_000498"
     CDS             27262..28659
                     /locus_tag="IWD16_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9087583.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            28818..29441
                     /locus_tag="IWD16_000499"
     CDS             28818..29441
                     /locus_tag="IWD16_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087584.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            30190..>32274
                     /locus_tag="IWD16_000500"
     CDS             30190..>32274
                     /locus_tag="IWD16_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9087585.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKN"
BASE COUNT        10897 a         5300 c         6677 g         9400 t
ORIGIN      
        1 gatctaaatt atgtgcagct tgactacgta ccaatctata ttacttacta gattggtcgt
       61 taatctattc ggatcgaatg agcaaaatat ttttgtgaat gtccatcctt gattatgatt
      121 ccaaaaagaa tcattgggag gctgtggcgt aaatattata ctataagcca gaaataccta
      181 tcattctgaa cttttttatt taggaacttt catctcaagg ctttttcaat taatttgtac
      241 ccctgttttt tggataagat catgtgcatt gattttttct atctctacgt tcatctccta
      301 cttaatgcct atctggctcc tgagggcttc tttttaaggc gtcgtcatag tcttggctga
      361 ggatttcttg gtaatctgag ggaatggctt cccaagtgag caggcgaacg ttcatgacgt
      421 gttttccttt gtgtttgatt acagcatggt ttttctcttt ttctacctct ccagcgtgaa
      481 aagacagcca tgtaccatgg ccaaagatat cattacatac ccaacgttcg atggaattgt
      541 ccacgtatcc tacagggagt gtggtatagc cgatttgtgg tttcaaaaaa ggattgaatc
      601 gatcgatcgt ttcatttttg tattgataat tgataagaaa aagatagacc aaacaaaaga
      661 cgagcagaaa gaaagatagt gtctttttga gtgagaaggg ttgttttgat gaataagatt
      721 tcttgttaac aagaggttca tctggcaatg ttggttctat tgattcggta gctgattcaa
      781 tagaaggaaa atcaatcggc agacctaaag tatgatgtaa aagtgacagc atttcaaggt
      841 ctggcattgt acgagaattt tcccaactag aaatcgtttt atcggaaaca ccaatttttt
      901 cgcctaattc tttttgcgta agcttacaat ttctgcggta agttcgtatt ttttctccgg
      961 tgttcatctt cttctccttt cgattctaca gtatatagat ttattctaca tgacctatac
     1021 acttgataga tacgtttttt tgatgaaaaa cggtatgatt ttatcaaaga atcatttaag
     1081 taagggagag tggtgataat caaaaaaaag ttcacagttt tactcacaat catagtgacc
     1141 ggtatcatga gtggttttgc cttggttagt ataaataaac aggtactcta tcggacaaat
     1201 gaatttttcc atcaaacaga tacttatact ccgattcgat tgccacaaca ttttattggg
     1261 caagaagaat atgagcagct aatcatggta tttgaaaatg tctcatcaga aactgagctt
     1321 tcttatatga aaaaaacagt aaatgaatcc aatgaagtag atacatcttg gaacttcaag
     1381 atacagccaa agcttgaaat gacattgtat tatcaagggg atgtcaaatc ttctcacgag
     1441 aaggtaccct attctaatac ctcctttcgc gtaacgtcca ttgaagaatc gatcaaaata
     1501 aaagattttg aaggtgaatt tttcttgatg acgagagaaa aagaagccta tgaacggttc
     1561 atttcactat tcactgatcg attcaatcaa gcatttcaca ctgattatgc cactaatatt
     1621 tttgaagact ttgaaaatag ctattcgtat tcggttattt caaatacaga cgtatataat
     1681 tatcgagtgt atttaactat tggattgatt cttttattcc ttttgttatc atcctttttc
     1741 ttattgatga ataaagaaat cagtttattg ttgaataatg acttttcatt acatcaagta
     1801 ttgtggtgtt tattggggaa aaagaaaatc attattacat tcataattgc aggaatcatt
     1861 ggtggtttta tttttatgta ttataaggag caatttcttt atttgttcca gatgtacgtt
     1921 ttcttgttca gcggaatata tggtatctct ttgatttctc ttttattagt tgaaaaaatc
     1981 gcttcgttaa gagaagcgtt caactatatc caatacattc tttttacatt tggattgttg
     2041 ttttctcttc ttttgaccgt tgccaatgtc gaattagcaa ctatcgtttt ttcttcttca
     2101 cagttagctg tttcccatga accaccaaaa gaaatcgctg gtgaggacta tcatgtattt
     2161 tatccagtaa ccatcgggaa aaaccacgtg gattttattt attccaatcg ttttgcatca
     2221 gcagcagatc aggaattata tgaaactttg aataaaaacg gaagtatatt agtcaatgta
     2281 tcagattact tgaatgaaga aaatgaacag tttggcagag ggataaaaat aaatctgaat
     2341 tatcttaaga aatttccttt gattgatgtc tctggaaggt tgatccaaat aaccgaaaaa
     2401 gaaacgcacc ctgttatatt gatccctgaa cgatatagac atacagattt aaaaaatgac
     2461 ctggcgcaaa taacagaata ttatcaagaa atcagtgaaa aagaaccgaa atttctattt
     2521 ataaaaaaca atcaacccat ttatacattt attccttcta tcccctggat cgaacattat
     2581 ccggttgttg aaatactgac attgaaaaac agtatgtatt tggaaagaaa tattctcagc
     2641 ggagaaatct atccaccgtt aaagatcaag ataggctctt tgtcgaaaga aaggttattt
     2701 gagctgattg aagaaagtca gttacgtgat aacttgcagt tatcatttcc gtatactcag
     2761 actgaagaga tagctgtcaa agtgttaagc agatcatttc attacttgat acagatattt
     2821 ttactcactt tcttttcttt cttcttgttg agctatgtca tgttatgcat atattttgtc
     2881 tacaattgtc ggaaaatcgc tatctttcgt tcaagtggtt attcgctctt tgaaacgtat
     2941 aaagatttct tcatgatgaa cctcatcaaa tggggaacga caagtgtaat cttccttttt
     3001 cttatagaaa gagagcctaa atatcttttt aatatttttt tctttagctt cattgatttt
     3061 attctttcag ttgtttttat tttgtctaca gaaaagaaaa gtcagttatt gctgatgaat
     3121 ggaggttaac aattggagcc gttgatagtg ataaaaggtg cgaaaaaaat cattcgaaac
     3181 aaaacgattc ttcatttgga tcgtttcact gcggatgctg gggatcttgt gactgttgtt
     3241 ggagaaagtg gagcaggtaa aagtacgtta ttgaatatat taggattgaa tgatcagttc
     3301 agttcaggac aattctattt gttcgatcaa gagacgaaac aattaactgg gagagagaag
     3361 cgcttgataa aaagaaatac catatctttt ctttttcaag attttgggtt gattgaatat
     3421 gaaacaatcg attttaattt agatattgga ttgaagtttt cgaatttaaa tgtttcagat
     3481 aagcgaacag caaagtatga ggcattgaaa aaagtaaata taaacaagaa gctaagtacg
     3541 cctatctaca cattatctgg tggtgagaag caaagaatcg cctgtgcgag agtcttattg
     3601 aagaatagca aaatcatttt agccgatgaa ccgactgggt cattggataa aagaaataag
     3661 gataaagtag ttgaaatctt atcgcaacaa gcttcccaag gtacgttagt tatcgtagtc
     3721 acgcatgatc catatctgac caaaatagga aacaagaaga tcatgcttta aaaatcaaaa
     3781 aggaaaggat gtcgttttat gaaaaaagca gttattattt gtgcactagg gataggtttt
     3841 gttggaggaa acgctatgtc atctgaagtg gaagcaaatt cagttcacgg tgaaatctat
     3901 tacaaatctt taaagccagg agagattcat tttgatgcta aaggtaatat tattaagtca
     3961 aatttaccag tagaccggga aatttctaga acagcaatta atcatagctc tggaagatgg
     4021 gtttattata gtgatacaac ttgtacgaga aaaacaggtc attctaacca ctatagtcgt
     4081 gtctataatc gtcacggtgc aaaagcgaaa gttggaaact ctattcgatc agcaaatgca
     4141 acaaaaggag tatgggccta tgcaagtgcg acaggaaata gaactgacac attctcatgt
     4201 tggtacaatc caaccggttg gtattaagta tccaaaagtt tatataaaat agaaaaaaca
     4261 tataatgaaa cagcgttaaa agttattttt ggtagtttta atatattcat ttttagttgt
     4321 ataaaaatat atcataataa aaacaacaaa aaaatgtctg gaaaatcgaa cataaaagtg
     4381 tctgatttga atatcccagc agcggaagaa gaaaaacaaa atcgataaca tttgttctaa
     4441 gaattagtta cgttaaatac ggaacgcaag atagacttga aaaacggtaa caaaagaagt
     4501 agtatattaa ggtgtgatgt gctacttgac atttattctg ctgagcgaac tttctggatt
     4561 gtgtagcctt atatctattc aggacgtatc gagcagatta gtaaaggtgt tcttgatgat
     4621 tttcatcttg aacacctttg tttttagcaa ttcgcaaaaa acgaaaagta ggagaattga
     4681 taaatgaaaa aaagattttt agcacttgcc atcgtactag gcactggctt attgtcaggc
     4741 tgtacgaatg ccggtgaaaa aacagccgtt agttataaag gcggtacaat cagcgaacaa
     4801 gaagtaatgg atagcttgaa aaaaatgcaa ggagcagatt ccgctgtgca acagttgatc
     4861 gtttatcaag tatttgaaga taaatatgga gatgacgtat caaccaaaga aatcgattct
     4921 caatatgatc aaacaaagaa acaattgggt gattcatttg attcacaatt gaaatctgca
     4981 ggttacacag aacaaacatt caaagacagt atcaaacaaa gtctagcctt tcaagaaggt
     5041 ctaaagaaac atatcaaatt gaccgatgaa gatttaaaaa cggcttggga atctttccat
     5101 ccagaagtgg aagctcaaat tatccaagta gcaagtgaag atgatgcaaa agatgtgaag
     5161 aaagctgctg ataagggaga cgatttctct aaattagcca aagacaagtc aactgatact
     5221 acgacaaaag aagatggcgg aaaagtgaaa tttgattcca ctactacaac ggttccggca
     5281 gaagtcaaag aagcagcctt taagttgaaa gatggtcaag tttctgatgt gatcacttct
     5341 accaatgctt caacttatac tacggaatat tatgtggtta aaatggtgaa aaaccaaaac
     5401 aaaggcaacg acatggataa atacaaaaaa gaattgaaag aaattgcaac agacacaaaa
     5461 ttaagtgaca gtaccttcca gaataaagta attggcgaag tattgaaaga cgctaatgta
     5521 aaaatcaaag acaaagactt tgaaaatgtt ttgagtacat tcacttcaga ttctagtaca
     5581 gcaagtagta aataagcggt ataaataaaa aatatgaact atttaaatca ataaaactgt
     5641 tcatctaaat ctttttatta gaactaaatc aacagtagtt taattactac ttgatttagt
     5701 tctttttatt ttaaaaattt tttttactta gaatccagcg accctagata tttttctgta
     5761 taatcttaaa taaagaaaac aatatcaaaa aatgcacgta gaagtagata aatggagtgg
     5821 ttttgcaaat ttcttttatt tctagaagtt attcacgtat acggatttgt ttttctcaca
     5881 aataaaaagg caggaagtac aatggatcat ttgtatataa aatatattca tcaattttcc
     5941 gttttggtgt taggcacact aattatagcg gggattgtcg catttattat tcaaggccgt
     6001 gacatattaa cagtacatca agagtttttc agttatgcat ttggtattcc tctgatcgct
     6061 ataaggtctg ataacaaaaa gtggaagaaa cgctattaca ttctcttgct atttttgtgt
     6121 ttttatctgc cacctaagat ttttacttta ttaggtatac aatgcctatc attttctttt
     6181 atttcctata tcattgtttt aattttcctc agtttcatag tatggatgtt ttatcttgtc
     6241 aaaaaataaa tgaatgtttc atgaacagta cttcatcaca gcctaatttt tttctttatg
     6301 ctatcgttat attaaagaaa tcaatcagaa gggaaagaaa gtgatgaaaa aagttttatt
     6361 agtggtaaac cccagttcag gcggtgaaca agcaaaggaa tttgagcaat tagcgatagc
     6421 aaaattggaa tcggtgtttg atgaagtggt cgtcttgcat acaaaaaaag caggggatgc
     6481 aaaaaacttt actcgcgaag cggctacgga agggtatcat agtgtgtttg tcatgggcgg
     6541 agatggaaca gtcaacgaag gaatcagtgg aatcgctgaa caagaacatc ggccaaattt
     6601 cggatttttc ccattgggta cggtgaatga cttggctcga gcattaggga taccgctaga
     6661 acctgaggaa gcgatcaatc atttttcgat tgaatcagtc aagccattgg atattggaaa
     6721 aataaatgac gactatttta tgaatgtcgt cgcaattggc tctatccctg aatcaatcaa
     6781 tgatgtagat gcggagaaga agacgaaatt tggaaaacta gcttatttca tgtcaggaat
     6841 caaacagtta gcttccacac aatcctactc gtttcacgta gaagttgatg gaaagaagga
     6901 agaaattgag agcagtacct tgctgatagg actaactaac tcagtcggcg gttttgaaac
     6961 attacttcca aatgcaaaag tggatgatgg taagctccat ttggtttatt taaaagattc
     7021 ttctttgatc gatacgctta aaacattgcc agacctatta aaaggagtag atgaatctac
     7081 gaaaaatcta gcctacctta cttgtgagca attagttgtg tcattagctg atgaggaaga
     7141 attgacgacg aatgttgatg gagatgaagg ggataaatta cctgtgacaa tcaaagtatt
     7201 gccctcacat ttacaggttt attgttaaaa tggaggttgt gaccgaggag ttggcgggag
     7261 aacaccgctt gttcgggata agggactgtg acaagacttt tgtcacagcc ttttttttgt
     7321 acaaaaaaga aatttacacc taatagaaaa atttttccat aaaaatacat attttaaata
     7381 tgaaagtaca attggaaata gattaaaacg ataaaataaa attgtcaaaa cgtcagagag
     7441 gatggtggag aatgaatcaa tgtgtaaaaa agctgattgc gtcgattttg tttgtcttcc
     7501 ctctcttgat ggtgacatcc gtttttgccg atgtagagaa aacaaacgaa tcaacgcaat
     7561 cagagaatca aagttttgac aagactactt tttcagaaga atcgattact tctgaaacta
     7621 cagaatctca aagtgaggct tctactaata aaaggcaaga agaaaataag acaagtgaga
     7681 cctcagaagt agctgaacaa acgtctgata gcagtgaagc ggcactggca agagctgttt
     7741 cagcggatgg gtacagcgaa gctgccacaa cagaagaact ggcgcagctt ctcgcagacg
     7801 aatcagtaac taagatccga ctgattcagc ctttgacttt agacagggaa ttagagatca
     7861 aacgagatat cgttattgat tttggtggat tcgctcacaa tttcggcaca caccatattt
     7921 atatcaatga gatacctcag tcgatcgagt tccagaattt taaaggaaca gctgcacatc
     7981 caggtggatt gacaccaaat atcgatggaa atgccattat cattgcttat atgaccgatt
     8041 tttggggaag cagatatcat tttacgggag aaataaaatt taccgggaca cttgatttgt
     8101 atgatgggtc caaattagga ctgatttacg ctcctcgtgc aactgtcaca ttagatggtg
     8161 tttccggtgt gttggatgtc caaccagtga aggaaggact gaatgccgct ccgggtaaag
     8221 cctatttttg tagaagctat caattgaatg tgatcaatgg tagtcaactt tatggaccgt
     8281 atctagggaa attctacggt tttctagatt caggagatga gactggtagt ggaaaaacag
     8341 cgccaggaat ccatatctta agcggttcaa aggtctctct tgattatgat cgggcagatg
     8401 gcgttagcga tgaaggagag gcagtcgata cgctacctgg taatgtcatg tttgaagtca
     8461 gtggttcggg aagcgaattt tcggtaaata caaagatcaa tattaccaat gacaacaatc
     8521 gcggaatcat ccaaatgaga ggttcaggat cgaaagtcac tgtatcgaat ggtgggaaaa
     8581 tcaccatcaa tacagcaact acgggcggat ttcgcttgca aggagattct agtcaaatcc
     8641 atgtatcaag tggtggacaa atctcggtca aaatggctgg agatgacgat caacccggga
     8701 acaatgggat gcgttttgta ggaaaaggcc tttctttgat cgtagaagga gccggatcag
     8761 ttatcgacat cgataagcag agtgggcgtt ccagcgcagt tcgttttgag aatggtacgc
     8821 aaaagctgac agtaaccaat ggaggctcat tgagggtaag aaatacaggt gatggcaaat
     8881 cttacgtgaa cagactcaat caaggaaatc aagcgatcca attttatgac gcttctggtt
     8941 ttctagaaag tccaggttca gctgatttta cagtcactgg agaaaaatca aatatcgata
     9001 tacgcgcaga ttccggagca actgtagata caactggaga tattgattta aattttacgg
     9061 caggtgaaaa aacttattta gtcatggtcg ggaaaacagg ggaagataag accggcatca
     9121 tcagtgggga tgtgttgaat gtggacttag aatctcctac ctattttgat ttccaaaaca
     9181 aacggattgg aagcgagacc aatggcatag gcggctgggt attccaagga aatggagatt
     9241 cttctttaaa gatcgcccat tcagaaatcg ctttatggcg aaaaacaggc gcaacattcg
     9301 ataactttga aaaagacccg gattattttt ctgatatgac agacttatcg atcaatggga
     9361 ccgacttagg caatttcgta tcaagcccta gtgcaggttt aacaaatgaa tttgctcaga
     9421 caggtgccgg aatgaaaaat tataatcgaa tcagtgccaa taatcagctc ccgatgattg
     9481 aatccttaag agtgccaacg aatgcagata aaaaaatcta tgggcatgtc gttgttcccg
     9541 aaggtgtcga ggcaaagcca agagatgctt ggacaggcga agtagaagtg actttacgta
     9601 ttacttacgc agatacgacg aaatcatctg tagaaattac tgctctcacg caaggacaaa
     9661 cagatgaaaa tgaaggggca ggatataacc catggggaga aggcgaacaa ggcggcttgt
     9721 tcgaagttca agtacccaat gatgagagat taaaaacgga ggatcaagtg caagttgttg
     9781 ctgctaaaaa agtaagaggt ggccaagaag tgacagaact ggactctgcc aaaacgacag
     9841 tcgatgtgat tcctcctgaa cctgctaatt taagtaccac attgatcaat gcagcttctc
     9901 gcacgatcac aggtacaggt gaagcaggag ccttggtttc attgaaaaaa aatcaagctg
     9961 ttttgtcaga aacaactatt gatgatacag gggaatttgt tctttctatc ccagaaaatc
    10021 aactttttgc aggagatgta ttagaaattg tattaaacga ccaagcagga gaagctactg
    10081 ctaagggtgt gcagaacaag ccaagtacca acgatgaaat tgggaatcac aatccaagtg
    10141 atacgccagt tgtttatcat gatgccccgc cttttgcacc tgctacgaag atcgtagtag
    10201 aaggtggtct ctcatttctt gccccttcca aactcgattt tggtcagatc aaagcaactg
    10261 gtttgacaca agaagcattt ggccaaatgg atatacagga aaaattatgg atttatgatc
    10321 agcgagaaac gaaaaatccg tggatcttaa cgataaaatt atctcagcct ttcacaaatg
    10381 atgcaggatt ttctatgcag gatgtcctat atttcaagaa aggagaggag tatcaattag
    10441 tgaatgaaga agaactggaa attgtcagag gacaaaattc aacgaatgtg caagcagatt
    10501 attcagaata tttaaataac ggtcgggcgt ttaaacttgt cttaaccaaa gaaaatcaga
    10561 tagctggtaa ttactcagca gagataacat ggacattagc agatgcgccg acagaataaa
    10621 gatcaaggag gatgattgat gaatcgaata tggaataaaa gtcatcgaaa caactgctct
    10681 ttacttatga tccttctcct tggattcatc ctttggcaga cacctgtcca tgcctccgaa
    10741 ttcaattttg cggtagattt tgaattaccg gctaatcaga ttgataagaa caaaacatat
    10801 ataaatgtaa ggatggagcc tgggaaaaca caggaaatcc cctacacggt ctttaaccga
    10861 acagataaat cagtaacagt tgagacattg atccactccg ctacgacgaa ttcaaacggt
    10921 gtgatcgaat atggagaatc aaaaagtaaa tttgatgaaa cattaagtta tgatatgaaa
    10981 aacttagcaa aaacagtggg agaaataact gttcctcctc aaagtgcggt cactgagaca
    11041 ctttcactga aagcgccaga aaaagagctg gctggtgtgg tcgcaggagg gatcacgttt
    11101 caagaaaaaa aagagaacgt ttctaacaaa agtacagttg gtgtcgtcaa tcagttttct
    11161 caagtgaaag caatcttgat ttgaaatagc gaaaaagagt cttccccaga tttgaaatta
    11221 aaaaaagctt atgcggatca aagcgatgga agaaatgtga ttaaggcgaa tcttcaaaat
    11281 ccaaatgctt cttatatgtt cggcttgact acatcggttg aaatctacaa aaaagaaaca
    11341 aaagagcctt acctttcaat aaaaaaagaa aaaatagatg tagcacccaa tacaaatttt
    11401 gatttacttg tcccattaga aggaaaaaaa ttagctccgg gacattatca agcggttgtg
    11461 aagggtgctt ggaaagaaaa acgctggaat ctccataccg attttgaaat aactgatgat
    11521 gcagcggacc aattgaatga aaaggatatt gatttaaaaa atcagccaga aacatttgac
    11581 tggcgatggc tgatacttgt tggagtcatt cttattggct tatttgctgg attatttatt
    11641 ttaaaagaaa gaaggaaaaa gaaatgaaaa aaacaattgc attaagtacc gttctagctg
    11701 gaagtctatt attatgtaca caaagtgttt ttgctgagaa taagagctca gccactacag
    11761 aaggaacgat ttctgtgacc aaaccagatg ctgcaaatcc tgaagacgtt gttccagttc
    11821 ctcctgttga tccaaatgat cctgatactc cttttacacc tgacgaacca aatcagggaa
    11881 catccggatt actgacgatt gaccaagctc cagatcttga ttttggaaca atcaagttag
    11941 gtacagcaaa aacagtatat gctgcattac aaagcggaac ggacagtact ggagcacaaa
    12001 cacaagtaca gaatttcgta caagtaacgg ataaaagcgg aaattatgcc ggttggaaat
    12061 tatcagtcaa acgaacagaa tttattggta caacatctga tacgaataaa ctaactggtt
    12121 caaaggtgaa attcaaaaac gccgtgattc gaaatagcgc aactaatagc agtggaatgc
    12181 catctaccgt ttctggaact ggcgctgaag gaatcgaaat cccagcagat caggcagtag
    12241 atctagtaac tgcaggagca aatgaaggga ttggcacatg gatttattca ttaggagaga
    12301 atgtggaaca agggagacaa tcagtggaat tatttgttcc tgtatccaat tatgctgtag
    12361 acaactacgt ttcaacattc acatggtcgt taacagatgc tcctacagaa gaggaagcgt
    12421 aaaatcatat caacgatttc agggagtgga gccgattcaa ggcccactcc ctgaaatctg
    12481 ctatgcttac gtctcagctc ttattcatca actcgaagca gttttttgga agtcatggaa
    12541 acgttccttt gtcttttcaa agatctttcg accaacgagt aaggagtcac cgttgaaaaa
    12601 ttctaacaca taatgagcag aatcaatttt ctgcaattgc gataagttga tacagtaaga
    12661 ttttaaaagt aaaaagtaag catacttttc taactctgtc ttcaattgtt ttaaagaagt
    12721 attcactaat ttatcttctg tagttgtttt gaccataact tggtttttca aacctttgat
    12781 actttcaata tataaaattt cccgaggata caggacgaga cttccacctt tttgatcaat
    12841 aaagatttta tcgacattta cgatctgctg ttctttccaa ttgatctgtt ctagcacttg
    12901 acgaatttgt tcagataaat tcctgtcttt tctgatatat ccaatcggaa ggattttccg
    12961 attgatgatc tctactgcaa aattcgtata agcggtgaaa aaaacaatat tactatgtgc
    13021 atttttttta cgtatttgtt cggcgacatc aatacctgtg agtgattgct tcaagtcgat
    13081 atcgataaag aaaacatcgt gattatcaat agtgcccaac tggatcaact gattgatttg
    13141 agagatagaa gtgagctgca cgatattctc gatatgatga ttgaccaatt ctctgatgat
    13201 ccattcgctg tgaaactggt tatcttctat gataaaatac tttttcatat gcaggctcct
    13261 attaaaaaaa tatgtgtaca ttttgaaaca aaacaatgga ggaataagat ggtcgcaaca
    13321 gtgacacttt atttgacctt tactttgatt ttagatttag gtatctattt attcttttcg
    13381 aaacaactgc tagtaaaaaa cagctttggt tgtatagctt tgatggtggt gacactttta
    13441 cttcctatcc ctaaagtgag ctttccttac ctagttggct tattattgat gtttcttttt
    13501 tattttacta cgaaaaacct gtttatcagc tgtttgatca gtaatctttt ttttgctatc
    13561 actggactca gcttttttct ggtttttgat ctgatggatt atttaggaaa gagacatctt
    13621 ggaacagcaa gtttttttgc actacttatc gcatttattt tctatctatt agtaaaaagt
    13681 atagacaaac agacgagatt tctcaacttg ctttttactt atccaactga acactatttt
    13741 ggaggtattt gtattttatt aagctggggc gtgatttact tggtattggg aagtgttcac
    13801 ttccgctcat ctaattatct gcttctatct cttttgtcga tagtcatcag ttttttttct
    13861 ttgttttttt ccattatgtt gatcagtttc caagtaaaag aaagacaaca tcaggaatcg
    13921 cttcaactgt atcaaataaa tgaagaatat taccatcatc tagaggaatt caaacatgat
    13981 tataaaagtc tactgttttc attaaaaatt actcttcagg aaaataaaaa agaaacgttg
    14041 gaatttttag aaatattaga agatcattct gcagctattt ttgaaaaacc gcaaatacaa
    14101 cagctaacaa atattgtatc cccagctgtc aggggtgtct ttttaaaatt tctagaaaaa
    14161 gcagaatcgg agaaaatccc gatccaagta gttattcctg tcgaagtgaa aacaatcccg
    14221 attgatctgg ttgtttttat tcgctgttta tctattttta tttctaacgc attcgatcat
    14281 cgtgtagccg atcagagtcc gattacgatc aatctgctgg aagaaaaaga gggatcacgt
    14341 tttagtatag cgaacaaggc aaatccaacg aatttgacgc tatcggagat ggtcaaaaga
    14401 ggaaaaacct ctaaaaaaaa tggcgggtta ggtctataca gtgcgaaaaa aatgttggat
    14461 gtttacgaaa atgcagaact aaaaatcgaa ttttcaaaac agaatcacta tttttttgta
    14521 gaaatgtatc tggcatctag aaagagcaaa tcagcctact gacttactgt caaaggataa
    14581 gtttgaggaa agtctacacg gagatagaga caacaacaca gcatattcaa aagaaacaga
    14641 agcgtgatga ccgttgtcac ccttctgttt cttttgctat gtagttaaac aaagtagttg
    14701 acacttccgc tacttagtat tagtacgaaa gtatacttca tgatactaag tagataagaa
    14761 gtgaaaagcg gaacccacga tcgtcaaaat gctaactgct ttgcttacac cagataaagg
    14821 gaagagcaat atcgcttatt ttaggcgtgg agaagcaagc aaatccctac gatcacaaaa
    14881 aaaatgctga cccagcagtt aagagagtta gaacaagatg aaagtatcca tcgaaaagcc
    14941 tataaccaag tcccgcctaa agtagagtac tcgctcaccg aagaaggaaa atcattaaga
    15001 gagattctag tagcgttgtc tgtttgggaa gaagaacgta tcgaaaaaca aaaagcctca
    15061 agggaagaag acacgctact gcatacacat ggtggctacc taaattattg attttctatg
    15121 acatttattt taaataaaag aaaacaggag tttttttaaa agcatcatct attttttatg
    15181 taaaaaaatg gtatgattaa tcaagtaaga agtgattgga agaaggcaag caaatgaata
    15241 gtatggaaaa agtcaaccag tttagagatg aacgtaattg gcgacaattt cataacgaaa
    15301 aagatttggc tatctctatt tctttggaag catctgaatt gttagaactt tttcaatgga
    15361 aaacgccgga agaagtaaaa gaaacacaat tagaacgaat caaagaagaa ctggcagatg
    15421 tattgatcta ttcatacatg atggcagaca accttcacct ggatctagat gagatcattg
    15481 aagaaaaatt gatcaaaaat aatttgaaat atcctgtaga aaaaagcgca ggtgtacgaa
    15541 aaaaatacac agagttgtag aaatgtgtca ttcggctgtt ggaaagagca aaagaaaaac
    15601 agtaaaatga gtctggaggg agagaaaatg aaaaaaaaag gaaaagcaaa aagatggcta
    15661 gtcaatatcc tcttgttcct cttgttatta gtaggactag ctttgatttt caatgaacag
    15721 atcaaggatt attttgtcag agagacagga gataaatatg ctatagcgaa tgtaacaaaa
    15781 gaagatctga aaaagaataa cgataaagat gtcagttttg actttgatgc ggtagaacca
    15841 atgacgacag aaggggtcat gaggtcgcaa atgagaggaa cagatctacc agtgattgct
    15901 agtatcgcag tcccttcagt ttcaatcaat ttaccggttt tcaaaggctt ggataatacg
    15961 tcactacttt acggagcagg aacattatct ccagatcaag aaatgggaaa aggaaattat
    16021 gccttagcaa gccatcgtgc aacgaaccct gaattgctgt ttacaccgct agaaaattta
    16081 gagatgggtg ccaagatcta tttgacagat ttagaaaatg tgtatacata caaaacattt
    16141 tttaaagaaa aagttgctcc tacagatacc caattactga atgaagtcga gggaaaagaa
    16201 attgtcacac taattacttg tggcgacatg gacgccgtca cgcgactagt tgtacaagga
    16261 gaactggaaa gtgttacatc gataaaagat gcgacagatg atatgagatc tgctttcaat
    16321 ctagagacaa aaacatttta ggatactggg acataagtcg ttacagatta aaaacagcca
    16381 aaggatatga atcgataagg aaaaaccaat ttgctgaaaa aaagagaaac gcaaagaggt
    16441 gcatctctga tttggttgtt ttctagatag acttgtgttc cagttttttt attttttata
    16501 tgaaaaggct tgcataaata aaaattcgat ggtatgatga ttttgaataa gaaatggatt
    16561 gttactgtac ggcaggcaaa accagaattc agctaagaaa cagtaggcga attctaggtt
    16621 tttttatttt tcttgagagg gcgaagcaaa atgaaaattg ataaaatttt gaataacaat
    16681 gtcgtcattt cgaaaaatgg attcggggaa gaagtcgttt gtatgggaaa aggactagct
    16741 tttcaaaaaa agattgggga cgagatttcg ccagaagcgg tccaaaaaga atttgtttta
    16801 agagattctt ttgctaaaaa ccaattccag cagctgatgg ctgatattcc ggcagaggag
    16861 atcgaatggg tcaaacaagt agtcgaactg gctgaagata aactaaaagt tgaattttcg
    16921 cctaatattt atctgacatt gaccgatcat cttcattatg cgatcacacg agcaaaagaa
    16981 aatatccaac tgcctaatcc attattattt gaaacaaaaa aattttatcc gaaagaatat
    17041 caagcagcga aagaggctct tttgttcgtg aaacaaaaga cgggtgtgga actttctgag
    17101 gatgaagccg gttttattgc ctttcatttt gtgaatagcc aacaaggaaa cgaagacatg
    17161 caagtgacga tgaccgctac cacaatggta agagatatac tgagtatcat cagtaaattc
    17221 ttcggcatcg tgttcgatga agaatctttg aattatcagc gtatcattac tcatctgcag
    17281 ttttttgccc agcgttatct caaaggggaa ggatctgacg agcaagatga atttctctac
    17341 gcattagttc aaggaaagta tccaaaagcg tttcattgtg tcgaacggat caatgagtat
    17401 cttcgacaaa cgcaaaaaca ggaaatgggg atcgcagagc agatttattt aacgattcat
    17461 attcaaagag tagtaagtga gaaaaaaagc atccaatcat aaggattgtt actgtatggc
    17521 aggcaaaacc tggattgaaa aaataggttc acgaatgttt gtgactgttt ttttcaatct
    17581 gggtttttat tttttaaata aaaaagaaag aaggaaaaaa caatggataa tcaagcagtt
    17641 ggtcgtcgtg tatgggaagc ggtaggagga caaaagaatg tcaaaagttt agtacattgt
    17701 gctacgcggt tgcgattcag attaaaagat gaatctttag cagatacgca aaagttaaaa
    17761 gaagacccgg acgtgatcca agtcgtacaa agtggcggtc agtatcaagt agtgattggc
    17821 agtaatgtag cagatgttta tcaatcgatc gtggatgagg aaggtcttgc agtttcggag
    17881 gatggaaaag aagagtccaa aaatattttg aatcgtctga tcgatattat ctctagtata
    17941 tttacacctt ttctaggggc gatggcagcc gccggggtat tgaaaggatt cttatcattg
    18001 gcaacggtga tgggctggct gacagctgat agcggtactt atcaaatatt gtttgctgca
    18061 gcagatggtg tctttacctt cttacctgtc atgcttgctt ttacagcagc taaaaagttc
    18121 aaagccaacc agtttttagc agtagcaatt gctatggcac ttgtgtatcc agctatcaca
    18181 gcagttgctg gtgcgggaga agctatcagt ttctttggga tcccagtgat tctttcacca
    18241 tctggttata cttcttctgt cataccaatc attctagccg tatgggttca aagtaaatta
    18301 gagccattcg taaaaaaagt gattccgcaa tttctgcaaa tgattttagt tccgcttgtc
    18361 gttttagtag tgatggttcc attaacattt cttgctttag gaccaatcgg gacggttgcc
    18421 ggtaatgctt taggcggtct cttcaactcg atttatggat ttagtccaat cgtagcaggt
    18481 ctaatcatgg gaagtttatg gcaagtattc gtgatgttcg gcatgcattg gggattcgta
    18541 ccgatcatgt ttttgaatat cgaacaatat gggtttgatg tattgatgcc aatgcttctt
    18601 ccagccatct tggcacaagg tggggctgct ttagctgtag cattgcgaac aaaagatacg
    18661 aaacttcgag cattagggat ttcttctaca gtgacttcgt tatttggaat cactgaacca
    18721 actgtgtatg gtgtgacatt acctttgaag aaaccattca ttgccgcttg tatttctgga
    18781 ggtattggtg gagctattat cggattttcc ggagtaaaag ctttctccag cagtttggtc
    18841 agcttattga cgattccgac tttcattaat acggtggatg gtgtagagtc aaatgtgaca
    18901 gtagcagtaa tagctacagg aattgctttt gttcttgcat tcgtaggtac attgatttta
    18961 ggatttgacg aacaaacaca agacaatcaa ttagaaaaca aacatgcgaa tgcaggaaaa
    19021 ccgattactt cagcacggca taccttaaaa agtcctttaa cgggtaaagt actgccattg
    19081 agtgaagtac cagaccaagt tttttcttct ggcgtgatgg gaaaaggtat cgctattgat
    19141 ccagaagtag gagaactagt tgcacctgca gacggagaga tcacgactat ttttccaact
    19201 ggacatgctg tagggatcac aacgactgac ggagcggaga ttttgattca tatcgggatg
    19261 gatacagtag aattaaatgg gaatggcttt gagatcttag tcaaacaagg ggatctcgtc
    19321 aaagcaggag atttattgat tcgttttgac atcgaagcaa ttagagcagc tggctatagc
    19381 gtaatcactc cagttgtcat tacgaatact gatgcatttg ccgatatact tgaattagac
    19441 caaaaagaga ttattgcaaa cgaagatgtt ctggcaattg taaaataagg aggaaatcag
    19501 gatgcaatca cgtttttcag aaaaattttt atggggtgga gcaactgctg ccaaatcaat
    19561 tagaaggggc ttatgatcga gacggaaaag gactatccgt tgcagatgct atgcctggag
    19621 gtaagcagcg ctttgcgatt atcggcagtg aagaatttga ctggacgatt gatcaagaaa
    19681 aatatattta cccgaatcat cgaggaatcg atcattacga tcgtttcaaa gaagatcttg
    19741 ccttgtttgc ggaaatggga ttcaaatgtt atcgtttttc gattgcctgg acacgaattt
    19801 ttccaaatgg agacgaaggt actcctaacg aagcgggatt agaattttat gatcagctga
    19861 ttgatgagtg cttaaaatat gatattgaac ctgtgatcac gatttctcat tacgaaatgc
    19921 ctcttcattt ggcaaaagaa tacggtggct ggaaaaatcg aaaactgatc gacttctatg
    19981 aacgatttgc tcagacagta ttagagagat acagcagtaa agtcaaatat tggatgactt
    20041 tcaatgaaat caattcggcc tttcactttc cagctttaag tcaaggatta gtaaaaagca
    20101 atggagcagg agagtaccaa aatattttcc aagcatggca taatcaattc gttgcaagta
    20161 gcaaagccgt aaaaatcggc cacgaattgc gttcggatat ccaaatcggc tgcatgatta
    20221 tctatgcaac aacttacagc atcgatgcca atccagtcaa tcaggcagca acaatgatcc
    20281 aaaaccaaga attcaatttt ttctgcacgg atgtccaagt acgtggagaa taccctgcat
    20341 acacagcgcg tacccataaa aaatatggag tcgatccgga aaaattagag caaacggaaa
    20401 aagacttcca gttgctaaaa gaatatccag tggactacat cggatttagt tattatatgt
    20461 ctacggcaat caatgagact gatccacaag cagcaacttc agaagggaac ttgctgggag
    20521 gagtaaaaaa tccgtttctt gaggcaagtg aatggggctg gcagatcgat ccagaagggc
    20581 tgagaatcgc attgaatgaa ttatacaatc gttaccagaa accattattc attgttgaaa
    20641 acggtcttgg agccattgat catatggaag aggatggcac gattattgat gattatcgga
    20701 tcgattattt acgtcgtcat atcgaagcga tggctaatgc agtagcagac ggtgtagatt
    20761 tgatgggcta tacgccttgg ggatgtatcg atcttgtcag tgcatcaaca ggagaaatga
    20821 gtaaacgtta cggctttatc tatgtggatc tagacgatga aggaaatggg acactgaatc
    20881 gatcaaagaa aaaatctttt gattggtata aagaagtaat tgcctcaaat ggcgtaaact
    20941 tgtaattaaa gcagctgaat gaaaaagaac acacttctca catgtgattt gtgttctttt
    21001 tttatttttt atgtgctatt cttttagtaa tagtaaagca aagggtgacg ttatgaaaag
    21061 gacacgtgta ctatacttgg aagtcgcaga taaaattaaa gaagatattt tctcaggaaa
    21121 atatccagtc ggttcgatgt tgccgacaga aagtgaatta gaagaactgt ttcaagtcag
    21181 caaaatcact gtccgaaagg caatcgaact gctggcatca gatgaatatg tcgcaaagaa
    21241 aagtggtcgt gggacgacgg ttctaagcaa tcgtccttac aatcgattat ccaaagcggc
    21301 aagctttacg caaatcttgg agaactcccg atatgaagtc cggaaagaaa cgctggattt
    21361 gcaattggtc catataaaga aggaagatcc tttgttttcc tttttcggtg agcaagctgt
    21421 ttgttttaag cgcttatatt atttgaacaa tcagccttat atttattttg ttcattatct
    21481 tccacaagaa atgatgcaat tcagcagaga agaatttggc aaagaatctt tgtatcgttt
    21541 gctgacgaaa aaaggatata ctattgactc attttctgat gatttctcgg cagtattttt
    21601 aacagaagaa gaacaaggaa ttctgaaaac agacgcacag atcggcatca aacggatacg
    21661 gaaatcgtct acgcaaattg gggaaatgat tgaatattcg gaggcggtct accatacgga
    21721 gctgcatcct taccatattg attatgaaac ataaataaaa aaagaggttg tgaaaaagct
    21781 gccctgcatc aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcgtttgg
    21841 ttaggcttat taccgcagat acacggctgt ttctggatta ccatttattc tggataaggg
    21901 ggtgtggcac gattttcaga aggaatctat gaaccaatca ctgtattgat tttagcttcg
    21961 atggtaaccc cattcggctt gacgatcgct tatttcttag ggaaaatcat tcgtaaaaat
    22021 atactcaatc gtcaagaaat cgatacattg aagaccgctt ttcctatggg gatctgtcag
    22081 atcacagagg gatgtttccc tatcgtattg aacgacttgg cacgcaatgt catagcaacg
    22141 ggagtcggtg ggacagtagg tggcggatta agcatgtttt ggggcgcaga tagccgtatt
    22201 ccggcatcgg gaatgtttgc agtcgctacg atgaccagac catgggcatt cattggtgca
    22261 ttgttagctg gttcttttgt cacaggaata accattttat tattgaaaaa accagtagat
    22321 ccgaatgcag aaatcatcca agaagaaaaa gaggaagaag acatatcttg ggatgatttg
    22381 acaattagtt aggagaggca gaatggatta caagaaaaaa gtacaataga tgtaccttga
    22441 gacaggaatg gtatgaaaga atcagagtta gaaagaatcg aatatacaga ttttggttta
    22501 ggagcgatcg aaactgtcca gagcatcggc accctccttg aaatgacgat gaaggaaaaa
    22561 tgagaacctt tcgttgtcga aagggtctgg tataccttat gtcgaaggaa aggaaacaaa
    22621 aaaactttcc tgtactattc caaaaaggaa agaaagttat tatacagtaa aaaaagaaat
    22681 tattcttcat gctagagatc agtacacgtt cgagcccaat atcaaacact agttccaggc
    22741 aggcgcaaca ggtgcggtta tcactgaatt ttcttccagc agtgacgatt caagtgatat
    22801 ttttacaaat ccagctattt atcgttaaca aaaagatttt taggagaagc tgatgaacaa
    22861 aaatgatcaa attttgatcg tttagttcgt cggctttttt atttagagaa acatcattta
    22921 tcagtgaaac ataacacgag aacaaaatga ttttttcgtt aataaaagaa acaagtattt
    22981 gttgtttaaa ataatacacg ttattggcat ataggacgaa atcccagtat cttctataag
    23041 taagtcattt catatcgatg aaaacacgtt atttattttt gttttatcta taaaatggct
    23101 gaaaaatgat ttttttctaa atactatata tagtggcgtc tatcctatat accactaaaa
    23161 aactacaaaa tctagtcttt tattttaaaa aaacacgtgt agaatgaaga tagacttttg
    23221 agttagggag tgttttttta tgaaacttac tgttttttgc ggttcccgtt ttggtaataa
    23281 agaaagttac aaatttattg cgcaaacgtt aggaaaatac atggcacaag aagaaatcga
    23341 actggtttac ggtggttcat actccgggat tatgggagtg attagtaaaa cagtactgga
    23401 aaataacgga aaagtcacag gtatctatcc gaatgggctt tttgaaactg aattgcctgg
    23461 aaaagatgtt actacattta ttcctacaga aacgatcgat gaaagaaagg tattattatt
    23521 tgaaaaggga gatgctgtcc tcgttttccc tggaggtcta ggtacattgg aggaattctc
    23581 acaacttctt tcttggatag caatcggtct gacgcccgac aagccaatcg gaatattgaa
    23641 tattggcggt tattacagcg gcttgcaaca attattagaa acttttgcaa aagaggattt
    23701 tatggataaa aaatggttgg atcaagtaat cttttccaac aatccattgg aactggtcaa
    23761 tgaattacga gcagtatcta ttaaaaatca gttattattg aaggaggcaa aataatgagt
    23821 caaatatatt tagcaggacc atttttttca gaggagcaaa tcgatcgagt aagtcgtatc
    23881 gaaaaagcgt tggaggaaaa caaaacagtc acaagtttct actctccacg tcatcaccaa
    23941 gaaagcaatt atgaactatt cagcgctgga tgggcgcagg aagtatacga aaaagatatg
    24001 gaagaactga caaatgcaga atttgttgtt gctatccttg actttgagca tcaaacaatc
    24061 gatccaggaa cggcttacga attaggtgtg gcaactatgt tgaaaaaacc gatgatcatt
    24121 gtacaagaag agacggtacc tacgaacttg atgattacac aaagtctcca tacgtattta
    24181 aaatcagacc aagctgtacg tgaatatgat tttgaaacat tgcctgttga aacttacgta
    24241 ggcgagtact tatagaagag gaagggttgt atgtcagtga ttttgatttc aggaacgatt
    24301 ggtgcaggta agagcagttt gacggatatg cttgcaaaag agattgattc taaaccattt
    24361 tatgaaaatg tcgaagataa cgaagtattg ccgctctttt attcaaatcc tgagcagtac
    24421 gcttttttat tgcaaatctt ctttttgaac aagcgatttt tagcaatgaa aaatgcttta
    24481 gtgaacgatg acaatgtgct agaccgatcc atctatgaag atagcctgtt gttccatcta
    24541 aacgctgatt taggtcgggt aacggatatc gaagtacagc agtacgatag cttattagat
    24601 acgatgctaa acgagttaga tgatgttgct ccaaaaaaac ggcctgattt gatggtccat
    24661 atcaaagttt ctttggacac gatgctagaa cgtatcaaaa aacgtgggcg tgactatgaa
    24721 cagctagaaa gcgatgagac gttatatacc tattatgaaa cgttgaacac ccgctataat
    24781 caatggtatg aagattttga tgtatgtcct aaaatacaag tggatgggga taaattcgat
    24841 tttgtagaaa atccagaaga tgcaaaaaag gtgatccgtc agatccaaga aaaattagct
    24901 gaattggaag gaaagcctgc tggaaaatat tttacggata accagcatgc aaaaggtcaa
    24961 gaattaggct aaaaataaaa aaccaaacga cgggaaagaa aaattactac gtgatgtaga
    25021 ataagccttt ctatcgtttg gttattttat attcaaatat ttgaaataaa taaatcttta
    25081 ctctttgatt tgtaaactga aatgatccac tgtttgtttt actgatgaag cttgatcaaa
    25141 ggaagaagct gagtttgaag ttcataaaaa tcagccatag tgagttttgc ttggatttgt
    25201 ttatcttctt ctacttttta cataattttg aacaggataa gattcaaatg ttttaaatga
    25261 atctttgact agtttgtgac agttgtcgca tatgctcaag cttccttggt cattaaattc
    25321 tgtatggata tttttgttgt tattacatgt acaataatca ggactatcga tcatccattt
    25381 cgtctccttt acaatcatct tgcttcttta ctatagcaga aaagaagggg agaaacaaaa
    25441 gaagatccaa cagacggcac tatagactgt agatgttgcc atcctcatcc ttcaaacggt
    25501 aattaccgat ttttgtaaat atgactgttg tttctttttt attttctttt cctgaaataa
    25561 tatattcgtc atctttggta gtgatcttga tatctgtcag cactgttttt tcttcagatg
    25621 attcagaaga atcagttcct tgtgtctgat acgtgcgtgt ccattccatt tttttatctg
    25681 taaatgtcaa ctgtcccgcg gataaatcca tatctttacg tacttttagc gtatatacac
    25741 tgttggtggt atctgctttt tccgctttta agacagaaga atcggttgtt tcgtccgagg
    25801 attggtcaga gccgccaaac aaatcacagc cagtcaatgt caaaagtccc gcagacaaaa
    25861 taaatagtac gattaatttc ttcaaaatac ttcccccatt tgataggata aagaaattat
    25921 accattggaa tgtaaaactt caaacggatg gaagagaaaa acgcacaaaa cacccacaaa
    25981 tagagtaaat tttagaataa aattttaaaa aggcaaaaaa tccgccactt tcagaaagcg
    26041 ttttaaaaaa gtgttgcaat cttttaaaaa aatgctatat taaaaatgaa ccggttcaaa
    26101 tacttgttta agtataatta atagtgagag gaagttgctc tactgaagac tttagttata
    26161 tggatattga tgacaagcaa cactatgcaa agaatgcggt catcataatc gaacaacaag
    26221 aaagagaaat caaatgtaac tggcaggagt gagaatgtat ggcatcgatc cgtgatgtgg
    26281 ctaagatggc tggtgtttca gtgggaacag tttcccgtta cttgaatggt cagcaactga
    26341 aggaaaagaa catgaaaaaa atagcagaag cgatttcagc gttggactat aaagaaaaca
    26401 tcattgctaa agggttgaag aataatcgaa gcttctctat tggacttttg ataaacggga
    26461 tttccagccg ttttgggtcg gaagttgtct cagggatcga aagagtagct gaagaaaatg
    26521 ggtatagctt attattaagc gggtttgccg atcagccaga aaaaattgaa caaaaaattg
    26581 aatacctgat gaagcatgtg atcgatgggc tgattgtctt cttatccgaa gaagagtggt
    26641 ctggatttga aatgctcaca aaaatgagta ttccagttat cgcattgaat tgtccagaag
    26701 gaccagcagg tattgataca atattagtga atgatcgaga aagtgtagca aaagtgatcg
    26761 ctcatcatgg tgaacaagga cataaaaaaa tcggatttat tgctgccact caaacagact
    26821 acgtagcaag agaacgttta gcaggagcca aagaagcggt ccaaaatgat ccatcgatcc
    26881 aattagaggt ctttacagga gactattcta gaaaaagcgg ctattacggg gcaaaagcat
    26941 tattagaaaa aggggcaact gctatttttg tcagcaatta caatatgtcg attggtgcca
    27001 tcgaatgttt caatcaagaa ggtgtcagaa tcgggaagga tattgcgttc agtcactatg
    27061 attatttaga taaaaacgat ctgagtatcc ttccgaaaat cacgatcacg ccaccaacag
    27121 ataaaatcgg ggaacgagca gcagaacagc tgttaaaacg aatcaaaaaa gaagaggaag
    27181 aaaatgaaaa agtgattgtg atggaaaata tcatacatgg aatcaactaa aacgaactaa
    27241 aaaagaaaaa gaggtgcttt gatgaaaaaa tatcaatttc cagcaaattt ttggtggggt
    27301 tctgcagcaa gtggcccaca aacagaagga aggatttcag gcgatggaaa aggagagaac
    27361 atttttgatt attggtacaa aaaagaaccg caaaagttct ttgatcaagt aggacctgaa
    27421 aaaacctcgc aagtttatac gaagtatcaa gaggatgtac agttgatgaa acagactggg
    27481 cataattcat ttcgaacatc tatccaatgg agccgtttga tacctgatga atctggaaca
    27541 atcaacgaag aagccgttcg tttctatcat cgttattttg atgagttgat tgaaaacggt
    27601 atcgaaccct ttgtgaatct gtatcatttc gatatgccga tgtatctgca agaaaaaggc
    27661 ggatggttga accgagaaac ggttgaagcc tatgaaaaat atgccaaaag ttgttttgaa
    27721 ttgtttggtt cgaaagtaaa aaaatggttt actcataatg aaccaatcgt tccagtcgaa
    27781 gcaggttatt tatatcgatg gcattatcca gaagagtcag atatgaaaaa agcgatccaa
    27841 gttggctatc atgaagcgtt ggcttcagcg ttagccatca aatcttacca tgaaatggag
    27901 caaggtggtg ttattggtat catcttgaat ctgacaccaa gctatccaag agacgaatca
    27961 aaccctgaag atgttaaagc agcgcaaata gcagatgcgt tcttcaatcg ttcattcctt
    28021 gatccagctg taaaaggcga gtttccaagt gaattgattg agatagtaaa agaacttgat
    28081 ctcatgcctg tgatccatgc agaggatttg acggttatca aagaaaatac agtagatctt
    28141 ttaggtatca attattacca gccacgccgg gtaaaagcaa aagaaacacc aatcgatact
    28201 aaaaacggtc caatgcctga ggattatttc gacaattatg aaatgcctgg tcgtaaaatg
    28261 aatccttatc gcggatggga gatctatgaa caaggaatct atgatatttt gatcaatgta
    28321 cgagatcatt atggaaatat cgactgcttt atttctgaaa acggtatggg tgtagaaaac
    28381 gaagcacgat ttatcaaaga agatggcatg atccacgatg attatcggat cgaatttgtc
    28441 caatctcatc tgaaatatgt ccatcaagcc attcaagaag gtgccaattg tttaggctat
    28501 catatgtgga catgtatgga taactggtcg tggaccaatg catataagaa tcgatatggc
    28561 tttatttcag tagacttagc acaagatgga aaaagaacaa tcaaaaaatc gggtcattgg
    28621 ttcaaagacg tatcagataa aaacggattt gaggcttaag aaagaatagt tgagagcaaa
    28681 tgcgtgaaat tctccattct taccattttg tgaaaaagaa gttcaattat ttacgatctt
    28741 tttcaacttt tttgtctttc tatttcattt tttgtatata atagaatagt caaaattaaa
    28801 taggagtgga ggaaagcatg aggaaaaaaa gaccgtttct tctcttttgg ttattcggtt
    28861 ttttcttact gcttatttta tccgcagcat tttcaggaaa aatgacagca gatcttgctc
    28921 agaaagaaac agaaaaaatc gtagcaagca aagaatcagt agataatttc aaaaaagaat
    28981 tctcaactta ttggtgggcg gagtataaaa taactgccaa ttacgaaaaa ggcgaattgg
    29041 acatttattt gccaaaagga aaagaagaaa atcaatttat taatatcgat gacgtactta
    29101 gtgcgatttc tctcagacaa tatcaattta aagacaagca tttgaccgtt ttgcttttga
    29161 ctcatgatgg cgacaaagta gcccgtttga aaaatggaaa agtgatcacc gcttattctg
    29221 aaaaagtaga caaaaaagtg gtcgccgatc gtttagcgga atggaaaaag acaaatgaac
    29281 aagtcaagaa aacaactgtc tcttctcaaa aaaacgagtc gacaggaaca agtccttcag
    29341 attctggcag tggacaaacg ataacgggaa gtgaagaaaa tcaagcctca attattacga
    29401 atgagtcatc acaaggacaa tatcaatcct atggacaatg aaaaacagag gttgtgaaat
    29461 cagcgttttg acctgagtat tagagaaaca aacggcaaaa tagttcctca tattttgccg
    29521 ttttttgagc taataccgaa ggtcagctga ttgaacaccg tgtatcaaga ggtcttgagc
    29581 ctgccgttta actctgagcc acaaggagca atttcaaaaa atagctcctt atatttttcg
    29641 tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttacca agaggttgta
    29701 aaattagcgt cctgacataa tctaataccg agggtcagct gattgaacac cgtgtatcaa
    29761 gaggtcttga gcctgccgtt taactctgag ccacaaggaa caatttcaaa aaacagcttc
    29821 tcatgtttta taaatattgt gacttgtggc cgaggagttg gcaggagaac accgtttaag
    29881 gaagaagttg tgaaaaagct gtcctgcatc aagaaataag gagctgcgac aagaattttg
    29941 tcacagcctc tttttttgta cgaataccga gaaactggct tttgaatacc gtggagtgaa
    30001 gcggtcacca caagactttt atctcttcat tagtttaatc gcttacatcg cagcggtttt
    30061 atgctgtgaa aagtggacgg aataatcttt agaaagaata aaaaaatctt aagataacaa
    30121 cttttttagg aaattttcat ttttcaggag caaaataaga aaaaatgtga atagagagaa
    30181 gtgatatacg tgacacaact taaaaaattg tgccaaaatc gtttagcaat cgttttgaca
    30241 gctgttttct ttttctggct gaagacgatc attgcctact atgcagattt ttcattaggt
    30301 gtggaaggta cgatccagta ctttatcttg tggatcaacc caattgcgac taccttattg
    30361 ttttttggcc tttctttgta tattaaaaag ccaaagccag cgttagctat tcttttgatc
    30421 attgatattt taaatacgct tttgttgtac ttgaatatta ttttttatcg ggaattcaca
    30481 gatttcatta cagtcaaatc tgtattaggc ttttcgaaag tgtcgcaagg attgtcagga
    30541 agctcctttt ctttgatgaa gccccatgat gtcatctact ggctggatat cgctgtgttc
    30601 attgggctac tggtttggtt gaaggtcaag aaaataccta tcaaaagcaa ccctgtcggc
    30661 aaaccgatgg ctatcgctgt tacctgtctt tcagggctta ttttttcagg aaatctagcg
    30721 ctgagcgaag ccaaccgtcc acaattgctg cagcgaacat ttgaccgttc atatattgtc
    30781 aaatatctag gaatcgatgc gtatactatt tatgacggaa tcaaaacagg aatgactagc
    30841 agtgttcgcg cgcatgcaag cagtaatggg attgatgaag ttttagatta tacaaagaaa
    30901 cattatgcag agccaaatcc cgaaacattt gggatcgcta aagggaaaaa tgtgatcgtt
    30961 ctccatttag aaagtttcca gcagtttttg attaatatga aggtagacgg acaagaagtc
    31021 acaccgtttt taaatagtat tttccagaat caagcaacga tcagctttga caatttcttt
    31081 catgaagtag gacagggaaa gacaagtgat gctgaaaata tgttggaaac tggcacattt
    31141 ggtttacctc aaggctcatt attcacagaa cttgggtcag acaatgtctt tcaagccgca
    31201 ccagcgatcc ttggacaaaa gcaaggctat acaagcgctg ttttccatgg gaatgtagct
    31261 agtttttgga atagagatca tgtatataaa aacttaggtt atgacaattt ctttgatcga
    31321 tcttattttg atgaatccga cgaaacgtta ggttatggga tattagataa agatttattc
    31381 agagaatctg cccaatatct tgagcatcta caacagccgt tttacacgaa atttctttcc
    31441 gtaaccaacc acacgcctta ttatacggat gacaagaatt tcgactttcc atctttgaat
    31501 actggcaata gcacagtaga taattatgtt cgaacggcac attatctgga tcaatcattg
    31561 gaacaatttt ttacgtattt gaaaaaatct ggtatttacc aaaattcaat ctttgtgatt
    31621 tacggggatc attttgggat ctcgaataca gataataaag atttagcaag cgctcttggt
    31681 aaagatccag atacatggga tgaatttgac aacgcacaga tgcaacgagt gccattgatg
    31741 atccatatgc ctggttatac taaagggaca gtcaatcatg aatacggtgg agaaatcgat
    31801 gttttgccga cattactaca tctattggga atcgatgata aagattatct tcattttggt
    31861 acagacttat tatcttctca gcatgatcaa gtagttgctt ttcgaaatgg gaactttgtg
    31921 acacccaaat acaccgtcct aggaggaaaa gcttacaata atcagacagg agaaatcatc
    31981 gatacgaaag cagcaggaat cgatgaggaa atctccaaag atcaggaaaa agtcaagact
    32041 gcgttaagtc tttcagataa gctgaatcag gaaaatctct tgcggtttta tgtgccaaac
    32101 ggatttgaga cgattgatcc gaaaaaatat gactataaaa atgaagcgga acgtaatgaa
    32161 gcaatcgaaa agcaaaaagg cgaacattcg acaagtgtct tttcaaaaaa tggtaataaa
    32221 acaacaacca acttatatcc agtaacaaga acagacgttg aacagcaaaa aaat
//
LOCUS       DACDMM010000014        31847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDMM010000014 DACDMM010000000
VERSION     DACDMM010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.014"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(152..2305)
                     /locus_tag="IWD16_000501"
     CDS             complement(152..2305)
                     /locus_tag="IWD16_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP9087586.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2599..3447)
                     /locus_tag="IWD16_000502"
     CDS             complement(2599..3447)
                     /locus_tag="IWD16_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9087587.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3671..5611)
                     /gene="thrS"
                     /locus_tag="IWD16_000503"
     CDS             complement(3671..5611)
                     /gene="thrS"
                     /locus_tag="IWD16_000503"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP9087588.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5948..6127)
                     /locus_tag="IWD16_000504"
     CDS             complement(5948..6127)
                     /locus_tag="IWD16_000504"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP9087589.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6174..8001)
                     /gene="cadA"
                     /locus_tag="IWD16_000505"
                     /pseudo
     CDS             complement(6174..8001)
                     /gene="cadA"
                     /locus_tag="IWD16_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
     gene            8279..10558
                     /locus_tag="IWD16_000506"
     CDS             8279..10558
                     /locus_tag="IWD16_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9087590.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10651..11298)
                     /locus_tag="IWD16_000507"
     CDS             complement(10651..11298)
                     /locus_tag="IWD16_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087591.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11477..12196)
                     /locus_tag="IWD16_000508"
     CDS             complement(11477..12196)
                     /locus_tag="IWD16_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9087592.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12327..13478)
                     /locus_tag="IWD16_000509"
     CDS             complement(12327..13478)
                     /locus_tag="IWD16_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9087593.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13695..14435)
                     /locus_tag="IWD16_000510"
     CDS             complement(13695..14435)
                     /locus_tag="IWD16_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9087594.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14439..14762)
                     /gene="rsfS"
                     /locus_tag="IWD16_000511"
     CDS             complement(14439..14762)
                     /gene="rsfS"
                     /locus_tag="IWD16_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP9087595.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14819..15412)
                     /locus_tag="IWD16_000512"
     CDS             complement(14819..15412)
                     /locus_tag="IWD16_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9087596.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15396..16043)
                     /locus_tag="IWD16_000513"
     CDS             complement(15396..16043)
                     /locus_tag="IWD16_000513"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP9087597.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16056..16367)
                     /gene="yhbY"
                     /locus_tag="IWD16_000514"
     CDS             complement(16056..16367)
                     /gene="yhbY"
                     /locus_tag="IWD16_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP9087598.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16383..17492)
                     /gene="yqeH"
                     /locus_tag="IWD16_000515"
     CDS             complement(16383..17492)
                     /gene="yqeH"
                     /locus_tag="IWD16_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP9087599.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17489..18016)
                     /locus_tag="IWD16_000516"
     CDS             complement(17489..18016)
                     /locus_tag="IWD16_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP9087600.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18196..18981)
                     /locus_tag="IWD16_000517"
     CDS             complement(18196..18981)
                     /locus_tag="IWD16_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP9087601.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18974..19843)
                     /locus_tag="IWD16_000518"
     CDS             complement(18974..19843)
                     /locus_tag="IWD16_000518"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP9087602.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19844..21223)
                     /locus_tag="IWD16_000519"
     CDS             complement(19844..21223)
                     /locus_tag="IWD16_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP9087603.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21231..21650)
                     /gene="fabZ"
                     /locus_tag="IWD16_000520"
     CDS             complement(21231..21650)
                     /gene="fabZ"
                     /locus_tag="IWD16_000520"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9087604.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21675..22151)
                     /gene="accB"
                     /locus_tag="IWD16_000521"
     CDS             complement(21675..22151)
                     /gene="accB"
                     /locus_tag="IWD16_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP9087605.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22154..23389)
                     /gene="fabF"
                     /locus_tag="IWD16_000522"
     CDS             complement(22154..23389)
                     /gene="fabF"
                     /locus_tag="IWD16_000522"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP9087606.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23408..24145)
                     /gene="fabG"
                     /locus_tag="IWD16_000523"
     CDS             complement(23408..24145)
                     /gene="fabG"
                     /locus_tag="IWD16_000523"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP9087607.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24157..25074)
                     /gene="fabD"
                     /locus_tag="IWD16_000524"
     CDS             complement(24157..25074)
                     /gene="fabD"
                     /locus_tag="IWD16_000524"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9087608.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25181..25405)
                     /locus_tag="IWD16_000525"
     CDS             complement(25181..25405)
                     /locus_tag="IWD16_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9087609.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25458..26420)
                     /locus_tag="IWD16_000526"
     CDS             complement(25458..26420)
                     /locus_tag="IWD16_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP9087610.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26430..26870)
                     /locus_tag="IWD16_000527"
     CDS             complement(26430..26870)
                     /locus_tag="IWD16_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9087611.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27176..28141
                     /locus_tag="IWD16_000528"
     CDS             27176..28141
                     /locus_tag="IWD16_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9087612.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28198..28428)
                     /locus_tag="IWD16_000529"
     CDS             complement(28198..28428)
                     /locus_tag="IWD16_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP9087613.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28514..29401)
                     /locus_tag="IWD16_000530"
     CDS             complement(28514..29401)
                     /locus_tag="IWD16_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9087614.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29490..30713)
                     /locus_tag="IWD16_000531"
     CDS             complement(29490..30713)
                     /locus_tag="IWD16_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9087615.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30730..31809)
                     /locus_tag="IWD16_000532"
     CDS             complement(30730..31809)
                     /locus_tag="IWD16_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9087616.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
BASE COUNT         8794 a         6972 c         5489 g        10592 t
ORIGIN      
        1 caatttttgt cacaaccttt tttgccgagt aaacggtgtt caaaagccag tttctcggta
       61 tgaagtcaaa tttaagcaaa aatgtgagaa gctattttca caaaattttt cttcatactc
      121 gaaactaagc ggctttttca cgacctcttt tttattttgc catgaacata tcataatacg
      181 cgtccattat ttctttagca atcgtcaagt ttatgtggtc ttgttcgtct aataagttcg
      241 gcaagacaac actgatggca atttccggat tgtctgttgg tccgtatgcc acgatattac
      301 tattgactgt cgcaatatct ggatgacctt ccgcaaccgt ttctgcagtc cctgtttttg
      361 cggaaaggtc cattttcgca ctggctaatg gtttggctgt tgtgtaagga tcggtaccat
      421 ggacaacttg gtggaatcct tcacggagga tcgccatatt ttctgcagaa atattgaccg
      481 tattttctac tgttgtgcca atttctttct tcacttcacc caagtttcca ttttcgtcat
      541 tgccatagat cgctttgact aggtgcggct gtactcgttt cccgccatta gcgactgttg
      601 aagcatattg agctaattgg attggtgtat atgtgtcaaa ctgtccaaat gccaaatcgg
      661 taaagttacc tggagcatac cattctttgc cattatccgc attgaacttc ttcatataat
      721 caacagaacg tgagatccca ggagattcat tcggcaaatc gatcccagtc gttgtaccaa
      781 gaccaaactc tttgaaagag tctctcaatt gctgataagc tttagcttct tcatctaaac
      841 taggtagtcc cattccaggt gtatagtcta gtcctaacat ctttaacgcc actttgatca
      901 tatacgtatt agaagacaat tccgatgctt tgactgcatc aagagcaact tgtccgcttc
      961 gattaaagac agagcttttt tctgaagcac cttgcagacg aatcggctca tcaatcaaaa
     1021 cttgatttcc gctgatcgcg ttacttgccc agcctgctgt caaagtacca gccttgacta
     1081 cagaccctgg atcaaaggcg ctagtaatcg tgcctaaagc attctcggtg atttctttgg
     1141 aaccttgttc atgactgaaa ccagtcattg ctagcacttc tcctgtctga ggattcatag
     1201 ccactgcata agcaccaggc gaatactgtg ctttgccgtt gttgatcaat gtttgataat
     1261 tccttttcaa aatttcttct acttttgatt ggaattctgc attcatagat aacattaaat
     1321 tagaaccttt ttccccagag aagatttctt tttgattcga aacatttcct tcattatcaa
     1381 gggaaacttc atattgggtt ttggttcctt gcaggacatc ttcatattgt ttttccaaat
     1441 aactttgtcc tacacggtca ttccgagaat aaccttttgc taggtatttc tctgcttcat
     1501 ctgctggaag tccttgcttt tcagtcgtca cactgccaag aatacttttc agtgaacttg
     1561 ctgcattata ttctcgtgtc cagtctgtgc cagtcgaaac tcctggaagt tcagaagcgc
     1621 gttctgccac aacagctaac tcttgatcag taacaccaga gtttttgata tagacagtgt
     1681 ttaattcata tgcactattc atccgtttaa agatggtagc aatttttaat tgctcctcgt
     1741 ttaaattaat ctcatcagct gtgactttat tgaccatcgc actatattgt tcgctgttcc
     1801 ctaattgctt ttcttttgcc gaaagtcttt ttgtggcttc tttcaaatgt ttgggatcag
     1861 ccagccaaaa atctttttta tctctatctg tcagattttc atctaccgga acatcgatca
     1921 gcgagttcag cttcgttgcg atttcaagca aatctttagc cgtaacttgt gttcctctcg
     1981 tatatgtgat ggcctgattc gctttattcg taactaaagc ttttcctgtg gagtcgtaga
     2041 tcattccgcg aggagtactt tcttggaccg taatcgtaga agtagacttc actcgcgcct
     2101 ccatctcttc gccgttcacg atctgcaaat aacccaatct agcaatcaac gcaacaaata
     2161 acgtaaatat aatgaaaaaa agaaaattta agcgaaaagg aacatgggac cggcgaaacg
     2221 ttttgttctt aggttcttgt gcaccttttc ttattttttc taaccaagaa gtatttaata
     2281 tttttttcat aaaatctttc atcatttcct atccttccgc ctatttacat tcatgcttca
     2341 ttgtacccaa aaacttctta ataaaaaaga aaaaatgggc ggagcaagaa attctttacc
     2401 taatttcaag ataaaatcaa ctttttgttt cacagcaatc acttaaagtt acatatccaa
     2461 ctattttttt cattaaaaaa taaacttatt tttgaaaatt atgttttctt aagtcgtgaa
     2521 acactttgat ggatcattta tttgtggaaa actacgtttt gttattctaa aaaacttata
     2581 taaaaaatag taaggttttt atttcgaatc cataaaatat tcttggattg cttctcgtgt
     2641 ttttttcact ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc
     2701 gatcgtagca aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt
     2761 tggtctagat gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg
     2821 cgtcaacgca atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc
     2881 tgcacttcta cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag
     2941 ttgcgggagc aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca
     3001 tttttgcaga atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt
     3061 ctctgccgat tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac
     3121 tgtttctttt atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga
     3181 ctcatttgct tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat
     3241 cgtgattttg aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt
     3301 cggcgcactc aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc
     3361 aatattaccg ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat
     3421 actttcaata cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt
     3481 aagtatacca tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa
     3541 tccgcccaac agaatatctt atcccatcaa tcccacagat tctattgagc atctgtgtct
     3601 ttgaggaata cctgttatct attgttcgga ttgctttcta tctactactt ttttacagga
     3661 aatttaccta ttagttttct ctgctgtaat ttttgacttc tgcttccata gcatcaataa
     3721 agatattcaa atcttctaca gctgtttctt tacttccata acgacgtacg ttcactgttg
     3781 catcttccaa ttccttgtct ccaacaacta attggtaagg gatcttttgt gtttgagagg
     3841 cacgtatctt atagcccatt ttttcattac gatcatcaac ttctacacgc aaccccttca
     3901 tttgcaaacg ttctttgatt tcataagcat aatcgccatg tgcatcaaca gaaacaggga
     3961 tgattgttgc ttgtataggt gccaaccatg ttggaaaagc gcctttgtat acttctgtta
     4021 aataagcgac aaaacgttcc attgtagaaa caattccgcg gtggataact actggacgat
     4081 gcgtattttc cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc aataagaagt
     4141 ctaattgtat agttgataat gtttcttctg taccaagagc tgttttgact tgaacatcta
     4201 gttttggtcc gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat
     4261 ccatagcggc tttcagcatt gcttgggctt tttcccacat tgcgtcatca tcaaaatatt
     4321 tatctgtatt gtttggatcg cgatagctca agcggaaacg ataatctgta atattgaagt
     4381 cagcatatac tgccaccatc aattcaagcg tacgtttaaa ttcatctttg atttgatcag
     4441 gacgaacaaa tgtatgacca tcattcaacg tcatttctcg tacacgttgt aaacctgaca
     4501 aagcacctga tttttcatag cgatgcatca ttcctaattc agcgatacga attggtaatt
     4561 cacgataaga atggatcgtg tttttgtaga ccatcatgtg atgagggcag ttcattgggc
     4621 gaagaacaag catttcgcca tctcccatat ccattggcgg gaacatgtct tcttgataat
     4681 gatcccagtg tccagatgtt ttgtacaact caacatctcc cattattggt gtatatacat
     4741 gttggtaacc tagactaact tctttgtcta cgatataacg ttcgatcgtg cgtcggattg
     4801 ttgccccttt tggaagccag aatggcaaac cagaacctac ttctggggaa accatgaaca
     4861 agtcaagttc ttttcctagt ttacgatggt ctctttcttt tgcttcttca cggagacgga
     4921 tatattcttt caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt
     4981 gattgtttga gtttccacgc caataagcac ctgccactga taataattta aatacttgta
     5041 tacggccagt ggatggaaca tgtggtccac gacacaaatc gatgaattcg ccttgttgat
     5101 aagcagtaat cacttcttct tctggcaatt cactaatcag ttctactttg tacggatcac
     5161 ttgcaaaaat ttctaatgct tcctgttttg aaagaacacg gcgttcgatc ggcagatttt
     5221 ctttgacgat tttcatcatt tctgcttcga ttgccggcaa atcttctgca gttacttggt
     5281 tttgtccatt atctgtatcg tagtaaaacc ctgaatcgat agctggtcca acgccaaagt
     5341 ggatatttgg atagaggcga cgcatggctt gggccatcaa atgagctgct gaatgacgga
     5401 ctaagccaag tgcatcttca tggtctggtg tgatgatctc aatacttgca tcttcctcga
     5461 ttggacgagt caaatcaacg agttttccgt tcactttacc agctaaagct ttttttgcta
     5521 agctttttga aatgctttca gcaattgcca atgttgttgt tccagactca aattctttga
     5581 ctgcaccatc tggaaaagta atttttatca taatagtttc ctccttgaaa tgttttgatt
     5641 gatcagatca tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa gagatactag
     5701 gacgaaaagt tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta
     5761 acggtccgac cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc
     5821 ctggaaaagt ttcagcctag cttttcctct cttatcgaag actctcaaat tcagttggct
     5881 ttctcatcaa tcaattatgt gtttatttaa ccactaataa atagaaaaat caagtgggaa
     5941 actaaactta ttttttccat tccccattta caccgtatgt tccttttttc atctcattga
     6001 tgatgacatg gatgtgttct tttggtgcct gcgtgttttt atgaacagct tccgtgatat
     6061 ctttcatcat attttctaat tgttccggac tgcgaccttc tactaattcg acatgtacga
     6121 atggcataat gctctctcct ctttctgatc gttctttttt caactcgatt ctatcatact
     6181 atgtttgtct tgggtactga atcaggaaaa atggctggat ctttttttgc tctcaaggca
     6241 ctcaaaatag agaccgtatc cacgacttct tgcaaaacag ctccgaataa tgcggggatt
     6301 agtcccgtac ttgcaattaa catcaaaaaa atacaaataa agatgccaat cagtacagac
     6361 tgtttcgcta ttctcatcgt atctcttgaa atcactactg cttttgctac acgcaagaga
     6421 tcatctttca agatgaccgc atctgcactt tcactagcag ctgtagaacc atgagccccc
     6481 attgcaatac cgatatctgc agcagcaagt gctggtgcgt cattcacgcc gtctcctacc
     6541 atcgcaacag gtctttgatc tttagggata ctttctaaaa tggtcagttt atcttgaggc
     6601 aagctacggg catgtacaga atctaattgg actttttctg caatcgtttg agcaatggca
     6661 gatccatccc cggtgatcat catcaaattt ttcacatcaa gttttcttag ctccttgatt
     6721 gtctttgctg cttctgggcg aatctgatcc gaaaatagga tcgttcctgc aaaagatcga
     6781 tcaattgtta catagattgc tgtattggac gtttctattt cttcagctga aacaaaagag
     6841 gcacgtccaa tctttacttc atgattattg actactgctt ggattccttg accggtcact
     6901 tctttcagtt gactaacagg atacagttgg ctgtttttca cgttgttcac taacgaacga
     6961 gctaagatat ggctagattc ctgttctgca ctagctgcat acagaagtag ctcttctttt
     7021 tttattccat tgactggctg aatcgcctct acttccaata tccctttggt aagcgttcct
     7081 gttttatcaa aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt tccagttttt
     7141 acaacgatcc cgtttttact tgcttgaacg gctcattcct gcaactaaag caatgggcgc
     7201 agctagaatc agtggacaag gtgatgccac taccagtacc tcagcaaatc gtaaagggtc
     7261 ttttgagaac caccaagaaa taccaccgat caaataagca atcaacgtga aagggactgc
     7321 ataacgatca gctaaacgga caaatcgggc aggcttttct tttgattctt cgactagttt
     7381 aataatcatt tgatactggc tgtctgcggc acgtttttca gcgatcattt ggatcgatcc
     7441 gtctccattt aaagaccctg acataatctg atctccaggt ttcttggttt ccgggcggga
     7501 ctcacctgtc aaagaagatt catcgaccga agattctcct ttggtcactc gaccatctgc
     7561 gggtacaagt tcgccaggct tgactagtaa ctggtcatca atatttattt catcgattac
     7621 tatatcctgc aattttcctt ctttgaatcg atgggcaatt cttggggtgt tatctaacaa
     7681 agactgcagt tctttacccg ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt
     7741 aagcatgatc aatatcatca aactagccca gtattctttg acggctaatg ttgcaaggat
     7801 tgcggtaatc gctaatacat ctacgccata tcttcctgaa cgtaacgtct tgatcatttc
     7861 caaaaacatc aagaaggcga gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc
     7921 cggctggtga aaaacaaatt cacttaataa agctatccca ccaataagca aagtaagtaa
     7981 aaattttttg aaatgtgtca ttgttttttc tcctctttta tgtgacttag agaggttagt
     8041 aataaatttc cagctgcctc tttcatctta agcatagtcc ttgactagca ctttttaaaa
     8101 caataggcct taattgaaaa caactatcac tatcagcgtt tccaaaattc atcagttcta
     8161 caaacaaaaa tattcagcaa ggattacttt ttcgattcat cagttaaaaa gtaccttttt
     8221 tcgaaaaact atgctatact tttaattcgt tatttacggc ggaaggagca aacatgcgat
     8281 gaatgagaaa caactagaac aaacgcacgt ggatgaaacg atcgaactaa tccagatcga
     8341 acaaaatatg ctggatacaa aacaacaaga attgactcag gaaatgaatc attcgacaaa
     8401 agacacagct aatcacaaga ttcgagctgg ttccaatgaa gctttttatg aatctgctgt
     8461 tgaatatcgc caacatgagc aggaattgct tttgaaatac catactcttg aagcacagca
     8521 aaaacgcctg caaacgttgt ctgtcatgaa agatagtcct tattttgccc gtatcgactt
     8581 taaagaagac cgtgaaacag aaacattata tctaggtatc gcttctttaa gagatctggc
     8641 agaagaaaca atcgttattg actggcgcgc accaattgcc aatctttatt atgaaggtga
     8701 gattggtccc gcattttatg aaactgaaac agatagaata gatgtcgatc tgcagttgaa
     8761 acgacaattt aaaatccaag aaggacgtat tttatctatg gtagatacgt cggaagtaat
     8821 caatgatgac tttttgctag agatattgga tgaatcatca agcaaccata tgaaaaacat
     8881 cgtctctacg atacaaaaag cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat
     8941 gctgattgaa ggaatcgccg gaagtggaaa gacatccgct ttgcttcaac gtattgcttt
     9001 tctgttgtat cataatcgca agtggctcga ggctgaaaac gttcttctat tttcacccaa
     9061 ccatttgttt tctgattaca tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc
     9121 tacacagaca ttcaaaaatt atttggtgca gctgctgcca gagttttctt tactagatga
     9181 acagcaacaa gaaagtggtt ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag
     9241 cggattgacc ttagtcgatc agattgatcg ctatatccaa ggcatcacaa actacggtcc
     9301 cttgttccgt gatatgaaaa taaatggccg tacgatccta tcaaaggaat cgattcgcca
     9361 atggtataaa gaaacaaatg aacaattacc tttacatcat agactttctc tattacaaac
     9421 gaagctcctg aaaaaattag gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa
     9481 agatctggcg gaagaacagc tccaagaact atacgcaaca gatccaaatc ttgaatatac
     9541 agaacaaaag gagcgttcat tacgtaaaaa actggccaac caaatcgtac gtaaaagatt
     9601 ccgtcgaatc catcgaggga tcatgaatta tcaatttgtc aatataacga aacaatatct
     9661 ccactttcta caaactgtgc cactttctct tctaacacga cataaaatca gcgagaccag
     9721 gtggcaggaa catttgacaa ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga
     9781 ggatgctgta ctattctttt tactgatgcg ccgaacatat ccagtgacgg ttacacacaa
     9841 agctcgctat atctttattg atgaaatgca ggatttcgca ccatcacaag tggcattgct
     9901 gcaagccttg ttcccaaaag caaatttgac tttctgtgga gatctaaatc aaaatgtctt
     9961 tggaaatgaa acgatcgttg gatcattgga agaccttttc gtggaccgtg aagtcactcg
    10021 tttccaatta acgacaagct atcgctccac aaaagaaatc actgattttg ctaatcattt
    10081 tattgtgaaa gaacatcaag tagaaacaac agcaagaaaa ggagaccttc ctttgctcat
    10141 ccaaggaaca acagatgata tcaaattgaa ttggttagca gataccattc gaacgactca
    10201 gggaaaagcc cggtattggc gtacagcaat catagctaaa acacaagaag aatgcgaagc
    10261 tctttatgct caactgccag acgagttgaa acaacaagtc cagctgattt taaatgagag
    10321 tgactttatg aagcgttcca ttattttact tccagccttt ttagcaaaag gtctggagtt
    10381 tgaccgagtt ttcttgtggg atgtagatgc agaacatttt tcttctcagc aagacaaatt
    10441 gatcctttac accatgtgta cacgtgcgat gcatgaattg acccttttta cctccaataa
    10501 tgaaagtcct ttgttaaaca atatggatat ttcatcttat gagaaaaaag tactgtaagt
    10561 aaaaagacaa ctgctatcat taatgttagc agttgtcttt ttatttacaa taataattgc
    10621 agaaaaagta attcaatcat ttaaggtcat ttattctata tataaatcca aatctgtttc
    10681 aggatatttt aatttgacaa gcgaaataat cattttcagt tcttccatgt tttctggata
    10741 ttctgggtca aataccttgc tatgacggcc aatcccgcca ttgtcgatca atcggtaaat
    10801 tcgattccat tcaatgaatt ggtcaattga ttggaaattg ctgattggat agccgccaca
    10861 atccaaaatc tctgatacac gaaatgctag gtatgcacta tcgatcactt tatttttttt
    10921 cgtgtctatc ttctcatttc tttcgataag aattttttca ggacgatgtt tcaagctcca
    10981 ctctaatagt agaaagaaag cctccatccg atactcttct tcaaataacc gaaaggctga
    11041 atcagaacga cgaaaaagca gcggaatcag ataattttgt ctataaatca gcattggtgc
    11101 gcggtccacc atacactcta aataacggtt cagataccct gtaccgacat agtttcctaa
    11161 tcgatagcct tcaacgccga atcttgcgta tttttgcgct aattttgaac aatcagttac
    11221 gttcacagct aactcttcta ctttgcggta accgcttaag atacgttcaa aaggttctaa
    11281 agtgattttt gcttccatat tcttccccgt ccatcttcca catacttcat atcatttcac
    11341 atgatactta tgctctcatc ataccacagg ttgaattaga aaagaaaata aatcacacaa
    11401 aaaagcaaga caatctgtta gtgagattgt cttgcttttc gaaaaagact gaatactttt
    11461 cgttaaaact ggttgcttag tcttctaatg ccacattatg gaacacatgc tgtacatctt
    11521 ctagatcttc cagtgcatca atcatttttt caaatgtttc ttgatcttct ggagatagtg
    11581 ttacctcgtt ttgagggatc atttggatct cagcgattga aaaatcagtg attccttttt
    11641 cttttaatgc ttcttgtata ctatggaaat ccgctacttc accgtaaacg atgatttgtc
    11701 cttcttcttc cattacatcg cgaacatcga tgtctttttc cattaggtat tcaaggatct
    11761 cgtcagcgtc ttctcctgca aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg
    11821 ctccggaaac acccatgttc ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg
    11881 ttcgattcac attgttcgtc aatgtatcaa caatcaccat tgagccgctt ggtccaaatc
    11941 cttcatagcg caattcagag tatgtttcat ctcctgcacc ttttgctttt tcgattgctc
    12001 ggtcaataat atgttttggt acattataag ttttagcgcg atcaatgacg aaacgtagtt
    12061 tttggtttgc atgcggatca ggatcacctg attttgctgc cgcgtagatt tcaatcccaa
    12121 attttgcata aatccggcta ttattagcat ctttagcagt tttctttgct acgatatttg
    12181 cccatttacg tcccatattt tcacttcgct ttctgatttg atagtttcat aacgcaaaaa
    12241 cgtcctgttc cattatactt gtgaatcacg attctgtgaa gctttttttc tttcttcctt
    12301 cattcttctc atttttagtc aattatctat actcggattg ggatttgccc gaaattctgt
    12361 tccggaatat tgcctcccaa ttgatagatt tgatcacttc taactaacag ttccgcttct
    12421 tgtgcctctt tttttccgat tcgggaataa atagaaatct cttttttttc ttttaagaat
    12481 cttcttccag catcggtaaa tccaagtagt cgaagataat ttttattttg ttggacagga
    12541 atctcttgct ttttgatatt cagcaagaca taacaagata atcgttggat ccgtgtccat
    12601 gtatatcttt ttgtttttac ctttgacaca aaatcctcaa aactatcagc agttgcagct
    12661 gctgttttca tgcgcgattc tattccttcg gtcatctgat agatatctgt cagttccagc
    12721 aaagtagaag ataacaaccg ataacgcaaa tacggccaat aagattccca agtctgcagc
    12781 gaacaagttg aaagatcttc ttctgtctga ggcggcacaa gtccagctat tctttttcct
    12841 tcttctaacg catggcgaat ggctgttgca ctggctatcc tctgatctgt gatgattttt
    12901 tcatgatagc ctgcactttt acgtcggatc ggatgtaatt ccatagggtg gacatacttg
    12961 gcattttcct ttgcataact taatcccaag atatgatttg gcgaagaaaa atcaagcgta
    13021 atatcgggat acagactgcg aaaaacctca gtcatttttt gcggataact atacgtttga
    13081 tcagacagtt cttgaaatgc ctcttctatc ctatcttgat tctcttggac aaagcgtccg
    13141 aaactgctgt aatcaaacga agtctcttct tctgttccaa aacacaaact gtcacagtcc
    13201 agtgtttgca agatttttac tgcaccttta gcaaaatagt cagctgactg aaccgcccat
    13261 tcgacgggaa gctccactac taaatccaca ccattttgta aagcttcatt cgcgcgatgc
    13321 catttatcga tgatagccgg ttcgcctctt tgcaaaaaat tcccgctcat tacagcaatg
    13381 acgacctctg ctcctgttat ttttcgtgcc atttccacat gatagcgatg tccattgtga
    13441 aaaggattgt actctacgat aatcccgcag cttttcatgc gcatcctcct tcatagttat
    13501 cgataaaaaa gaaatagaga caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact
    13561 atataaacta ttctcgcaaa cacaatactt ctgcgacgaa aagctgcact gcaagagcaa
    13621 tgcctatatc gttcggatat ataatttatg ttgaagattt attcttttct tgttaactcg
    13681 agcaggcaaa accgctagtc tttgtatgct acaaagaacc agcgctttga tttctctgat
    13741 gctgcttcgt ctgtaaaatc agaaaatgct tcgactttcg aaaagccgct gttttccagc
    13801 attcttaaat aactttccaa tggataggta cgctcttgat gtagttcatc ttcacgaata
    13861 aatcgaccag acccttcttg ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt
    13921 ttctcgcttc ctggataact atcccacaaa aaagcaaaat tatccccttg ataatgatag
    13981 ctatattctg gaaaaacctc atctacttga taggtggagt gtacgtcaaa taaaaatcgg
    14041 ccattttctt ctaacgcttg atagacttca tcaaacactt gctgtacctc ttgtctattt
    14101 ttcatatagc acagcgaatc agaaaaacag gtaattgcct gatattgtcc gatttctgat
    14161 aaatcgagca tattgccttc cacaaattgg atcgatgcgt cttcttcata tgctcgctgg
    14221 ctggcaatca tcaacatctc ttctgaaagg tctaaccctg tcactttata tccatctttt
    14281 gcaaaggcaa ccgctaatgc tccagtaccg catgccagtt ccaaaatatt ttttgtgtct
    14341 tcttgtaaat gacgtttcga aaaagctaac cattgctgat aaagtgtttc gtccatcact
    14401 tcatcgtaaa tgaaagcaaa tgtttcataa gccatatgtt aatccaacca ttcactaaga
    14461 tcgactagcg gagcatctga ccatagtttt tccagattat agaaatttcg ttctggcgca
    14521 tggaaaacat gtacgatcac atctccaaga tcgatcaata cccattttcc gccttctttt
    14581 ccttcaatac gttttacttc atatttgttt tcttcttctt tatcaatgat ttcttctgta
    14641 attgcgttga tttgacgttc gctatttgct gaacagatca tgaaataatc tgccaacaag
    14701 gaaacatttc gaacgtctaa agcgataatg tcttctgccc gttttgaatc tgctgcttca
    14761 atcgcaattt ttaattgttc tttactatct atgatgattt cctcctaata tgagcagctt
    14821 atttttttgc tacccagtaa ttatatgttt cgattgtttt tggataaatc tgttgttctt
    14881 gcgcaagcaa atgggccaat gtatgttttg tttcaaatgc tacagcttcg tctagatcag
    14941 cccatgcaat ggctcgtgca tcttgtaccc caggaaaatc tcgacctggt tcaatatagt
    15001 ctgctacata aatgattttg tcaaggagac tcatcttagc agccccagtc gtatgcaccc
    15061 ggattgcatg aagaatctct gcatcggtga ttcctaattc ccgctctaca aacgaagcgc
    15121 caacaagacc atgccagatt gcattattat aattcaataa ctccggatca aaaccgtctc
    15181 ttacgattac cattttgaac tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg
    15241 cagcaatact agctttttca ggagaagcac cgtatttttt ggcaagagcc acggctgttt
    15301 cttctactcc aagcacatgc ttgaagcgtt gttcgctcat tctcatctgt attttttgca
    15361 tcaggttttc tctagaataa gcagtatatt cattactata gtccatattc atacaaccct
    15421 tcattttgaa tataatcaat tactttatct ggaacaaggt agcggataga acagccttct
    15481 ttgatctttt gacgtatttt cgtggaactg atgtcgatct caggtacatc tacccaaatt
    15541 acaggatatg gagtatccgt tgtgtatcct ggtcgacgta tacccacaaa gttgaccata
    15601 gaagtcagct catcgatttt ataccatttt ggcaaatatt ctaccatatc cccaccgata
    15661 ataaagtaat aatctgtatc cggattgttt tgcgtcaatt ccttcatagt atcataggtg
    15721 taacttttcc caccacgagc taattcaatc gtttcaatct gcaaaaaagg attgtcttcc
    15781 accgctaatt ccaacatgtt gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca
    15841 tgaggtggct gataagaagg catcaaaaat acgcggtcaa gacctaaatt ccggccagct
    15901 tgttcagcca tcactaaatg tgcaaggtga acgggattga atgttccgcc caagatccct
    15961 acttgctttt tcttctctaa gaattgaggc atttcttcag gaaataattg tgaacgaaca
    16021 aatgtcttag cttgcgtatt catcatggtc actccttata ttgcttttac ttcttttgat
    16081 agcctttgat acttttcttt tgtggaaggt ttgtataata caataacacg tccaatgatt
    16141 tggacgattt cgcaatctat ttctttttct aatacttgag ctgcttcttc tgcaatctca
    16201 tctgtatttt gaagtaacga tactttgatc agttctcttt tttctaaagc ttcttcaatt
    16261 tgaacgatca ttgctgaatt gatcccacct tttccgattt gaaaaatcgg ctgaagatga
    16321 tgcgctttgc tacgtaaaaa acgcttttgt tttcctctta aactcataat tttccttctt
    16381 ccttaaatca atgctttgcg tttcaataca tctacaccct ttggcgccca gcccgcaatt
    16441 actactggcg ctgttacagt gatccagcct agaccggcaa aaacgatgtc tgttttttct
    16501 ttcacagaaa actcaaaacg aacaagctcc ggaaatgcct cgacttcatc aggacgagga
    16561 ggctgtaata atccacctac atgtttttga taaaattcgt ctgctttttc cagtttcgtt
    16621 cgatgaatat ttacatcatt tgacacataa gcaataaatg aagcacgatt gccgctgata
    16681 aaatcaaatc ttgcaagtcc gcccaagaaa agtgtttgcc cttcattcag ctgataaact
    16741 ttaggtttga tttcttttaa cggtgcaatg atccgcaaat cttttttacc taagtagtgc
    16801 gccatttgat ggcgatggat aattcccggt gtatcaatca aaaaatgccc gtcatctaaa
    16861 ggaatctcga ttttatccaa ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt
    16921 actcccgctg tatttttgat gatttgattg ataagcgtcg attttccgac atttgttact
    16981 ccgacgatat agacatcccg tccatcgcgg tattcttcga ttttttctaa tagttccgtc
    17041 atctcatgag atttcttagc actagttaat aatacatcta ctggacgaat tcctgcttcg
    17101 taagcacgtt ccttcatcca ttgtgttaat ttagtttttt tcaatgattt aggtaatata
    17161 tccactttgt tcccaactaa aagtactgga ttgtcaccga caaaacggtg tagtcctggg
    17221 attagtgaac cattgaagtc aaaaatatcc accacattga cgatcaatgc atctttttgc
    17281 cctaattcat tcagtaagcg caaaaagtcg tcatccgtca atgagacatc ttgggtctca
    17341 ttataatggc gtaatctgaa acaacgctga caatagactt ccccggaagc taaacctttt
    17401 tccaaagccg tttgcggcgt gtatcctaat tcaccgggtt tgtttgtttg tataacggca
    17461 ccacagccga tacattgtaa ttcttctgtc attcgattcc acctcgccag cccatatcag
    17521 ggtgttttgc taatagatag gccataattc ttttttccca aaaacgatta aattgtgtct
    17581 tccaactatc tgttgcgata atgggtttta caaggatgct acgtatccct gcacgattgg
    17641 ctccacgtat atcagtcata atctgatcgc ctaccattac tatctcatca tctgctaaat
    17701 ccaattgttt ctttgctatg ttgatccctc ttgctaaagg tttcattgct cgtgcaacat
    17761 acaaaagatc aaatttttca atcgctcgcg cgacacgttt tgaattattg tttgaaacaa
    17821 caacaacagg gattcctgca tttttcattt ctaaaatcca agtaagcaac tcttctgttc
    17881 catctggatt attccatgca atcaacgtat tgtctaaatc ggtcaatact gctttgatgc
    17941 ctagattctt taattgggca ggtgtaatct gatagatggc ttctaccatc catgttggtt
    18001 tgtaatttga aaacatgctg tctcctcatc tgcaaaaaat gaaggagcac ataaagtact
    18061 cctcaacccc gtattatact gcatatcagc aaaaaaaact acctctatac tcgttcgtct
    18121 ttcagaaaaa gcatttcttt acaaaatgac caaaaataaa gattgatcac agaaaaattt
    18181 agaaacaaaa aacaactaat attggcggaa cctcttgtat cgtttttcca gtaatttatg
    18241 ctgatctaat cgaacaagtt ccgtcaatct ttcaatcaac gcttttttca taatgtgtgc
    18301 gactttatat cgtggcaact gctcgccgtt gaacgtttca ggaataactc gatcaacgat
    18361 tcctaactcg cgtagttctg gcgcagtaat tttcatcaac tcagctgctt ctgcggcgcg
    18421 actaccgtct ttccacaaaa tcgacgcaaa tccttctgga gaaagcacag cataaatcgt
    18481 ttcttccagc atccagactt catccgctaa acctagagca agggcaccgc cacttccacc
    18541 ttcaccaatg atgatggcga tgatcggtac ctttaaatca gacatttcga gcagatttct
    18601 ggcaatagcc tctccttcgc cgcgctcttc tgctcctaca ccgcaatatg cacctgctgt
    18661 attgatcaag gtaatcaccg gtctattgaa cttttccgct tgtttcatca gtcgtaaagc
    18721 ttttcgatag ccttcaggat ggggagaacc aaaatttctg cggatatttt cctgcagatc
    18781 ccgtcctttt tgaataccga tcactgttac cggtttgcca tctaatcgag cgattccacc
    18841 aacaatcgct tcgtcatctg caaataaacg atcaccatgg cattcctcga aagaatcgaa
    18901 gatctcttca aaaaaatcca agcttgtcca tcgttcttta gctcttgcta actgaacgac
    18961 atcatgtgct gtcttatcca cgatctatcc atcctttctg tgtatgcaat tcaagaagcg
    19021 tatggatttt ctgtttcaat tccatgcggg gaacaatctg atcgacaaaa ccatgagata
    19081 ataagaattc agccttttgg aaatcttctg gaagttcctg cttgatcgtc tgttcgatga
    19141 cgcgtcttcc agcaaaaccg atcaagcttt ggggttctgc tagaataata tcgccttcca
    19201 ttgcaaaact ggcagttact cctccggtcg tcgggtccgt taaaacagtt agataaaaaa
    19261 gaccttcatt gctatgccgt ttgacagcag cagagatttt tgccatctgc attagagaaa
    19321 agattccttc ttgcatccgt gcgccacctg aagctgtgaa caagacgact ggaagacttt
    19381 cctttgttgc tcgttcgaat aatctggtga tcttttctcc gacaatcgtt cccatacttc
    19441 ccatgataaa attaggatcc atcactccaa taatgaaggg aatcccctga atggttgctt
    19501 taccagtgag gactgcttca tgaagtcctg ttttatcttg cattttttta attttttccg
    19561 tatattcggg aaatgctagc ggatctttgg ttacaagatc cgtatcccat tcttcaaaac
    19621 ttttttcatc aataatcaaa gctagacgct gccatgcacc gattcgaaaa gcgtatccgc
    19681 aatgagggca tactttttct tcaccgatgt ctttcgtata aaggatatgt ttgcaatttg
    19741 gacactttgc ccacatgtta tcaggaacag atggcgagtt agcaggtttg ctctctctat
    19801 taggattgat ccgaatatat ttctttttct taaacagagc catctatgcg cctcctatct
    19861 ccgtttctgg tgtccaattc ggtaaaaatt cttcttgtaa aaatgctgtg ctgtaatcac
    19921 ctgctatgac tgcaggatga ctaatcaaat ccatctgaaa ttctgcgttc gtgatgattc
    19981 cttctgtgat aaattcactt aaggctcgct gcatcttcat taatgcttca aaacgcgtat
    20041 ttccatggac gattattttt gcaatcattg aatcataata tggcgggatc gtatatcccg
    20101 ggtaaactgc actgtctact cgcagtccca tcccacctgc tggaagcagt aagttttgga
    20161 tcttccctgg agaaggagca aaatggaaag caggattttc tgcattaatc cggcattcga
    20221 ttgcgtgtcc ttggaatact acttcttctt gacacacatt caaaggctca ccggctgcta
    20281 tctccacctg tttttttact aaatcgattc ctgtgaccat ttctgttact ggatgttcca
    20341 cttggattcg agtattcatt tccatgaaat aaaaatcgcc ttcttcatcc atcagaaact
    20401 cgatcgttcc ggcattttca taattgactg ctttagctgc tcgaacagca gcgtctccta
    20461 gtgcttctct ttttgtttgt gagatcacta cagaaggaga ttcttccagt actttttgat
    20521 tgttgcgttg taaagaacaa tctcgctcgc ctaaatgaat gacatgacca aatcgatcac
    20581 ctaagatctg aacttctata tggcgagcag gataaatgat tttttccaaa tacatatcat
    20641 catttccaaa tgccgctgct gcttcttgct gagctgaaga aaaatgctgt gggagctctt
    20701 cttttgattg aactttccga atgcctttcc cgccaccacc tgcagctgct ttcagcatca
    20761 ctggataacc aattctgtct gctatttcca atgcttcttc tactgtagat aatacaccag
    20821 tacttcctgg aatgactggg acatttgctt cttgcatcaa ttgacgcgca ttgatcttat
    20881 ttcccattgc atcgatcgtt gcacttttag gaccaataaa tgtgatattg cactcttcac
    20941 acatggaggc aaatcggcta ttttcagata aaaaaccaaa tcctggatga attgcttcgg
    21001 cttttgtcac gattgcagcg ctcagtactt cctgaacatt cagatatgaa tcaacggctt
    21061 ttgccgggcc aatgcagatt gcttcatctg ctaactgtgt atgaagtgct tcggcatctg
    21121 cttctgaata tatcgctacg gtttggatcc ctagttcgcg gcatgcacga atgatacgta
    21181 cagcgatttc accgcggtta gctatcaata ctttagaaaa catatttcac ctatccaatc
    21241 ataaaggtta attccgcttc ggctactttt ttaccgtcta ctttagcgat tcctttgcct
    21301 attccggctg aagctttcac tttcaatatc tcaacttcaa ggtataaggt atctcccggt
    21361 gtaactttct tacggaattt tgctttgtcc aatccgccga aataagccgt ctttcccttg
    21421 aattgttcaa gagaaagtaa tgcaactgct cctgcttgag ccattgcttc aacaatcaat
    21481 acccctggca taacaggttc ttctggaaaa tgtccttgaa aaaagggctc attgatcgtt
    21541 acatttttct tagcaacgat ccgttctcct tcaaccattt cttccactcg atcaatcaaa
    21601 agcattggat aacgatgtgg aatgatttct tttatttctt gtatgttcat tgttttccct
    21661 ctttcctgct ttgcttattt ttgctgaatc ctaaagagtg gctgtccata ttcgacaact
    21721 gactcgtttt ctacaaggat ttctgtgatc actccgtcgg cctcactagt tatctcattc
    21781 atcagtttca tcgcttcaat aatgcagata acttctcctt tggcaacttg atcacctact
    21841 tttttgaaag ctggtttgtc gggcgctggt tgcagataaa cgattcccac gattggcgaa
    21901 acaacctcga ttccatcaaa tactgccgat tctttttctt tcattatttc tggagcagtt
    21961 gttgcttgtt caggtatgac attgactggc tccgaaactg gcaacgtttc gtttgtttga
    22021 accatattca tgcctcgact tgttttattt ttattcatat acaattcaaa ttgaccttca
    22081 cgcaaatcga attccgttag cgaggattga tcaaattgac tgactaattc tctgatttca
    22141 ttgatatcca aaattattcc tcccagcgtt tcatgcacag aaccgcatta tgtccgccaa
    22201 accctaatga attactcatt gcataagtaa aacttgcagg tttgctttcg tttttgacaa
    22261 ctgtcaagtc aatctcagga tcttgttttt ctacattgat cgtaggtggg ataaattgat
    22321 gttctaatgc cagcaaagtt gccaaggctt cgatcccgcc ggctgctccc aataaatgtc
    22381 cagtcatcga tttcgtgctg ctgacgtaag tatctctgaa attttcacct aatgcgattt
    22441 ggattgcttt tgcttctgcc tggtcattgg ctggtgtact tgttccgtga gcattgatat
    22501 atccgacttg ttccggctgt acaccagctt cttccatcgc aagcttcatt gctttagatg
    22561 caccagaacc atctggagta ggagctgtca tatggtaagc gtcacagttt gcaccgtatc
    22621 caacgatttc tccatagatt ttggcaccgc gagcttgtgc atgttctaaa gattcaagaa
    22681 ctagaacgcc tgctccttca cccatcacaa aaccattccg attttcgtca aacggaatcg
    22741 aaccacgatt aggatcagtt gattctgaga gtgctgttaa agctgcaaat ccagcaatcc
    22801 ccatctcaca aatcgttgct tctgttcctc cagcaagaat gacatcagaa tagccatgtt
    22861 tgatgttacg gaatgcttcc ccaattgaat tcgttccgct agcacaagct gttacgatag
    22921 ctgtacaaat accttttgca cctacacgca aagcaatatt tccagctgcc atgttggcaa
    22981 tcgccattgg tacgaacatt ggagaaactc gttttgcacc tttttcatgt agacgaataa
    23041 cttggttttc gattgttgga agaccaccga tcccagatcc gatgatgacg ccaaaccggt
    23101 cttgattcat tttttcttta tctaaaccgc tcatctccat tgcttctaaa gcagcgtaga
    23161 ttccatagat cgaaaacata tccattcgtt tgctatcttt tttaataaaa tatttatcgt
    23221 aagggaaatc tttgacttca cccgctaagg taatccctgt ttctgaagca tcaaatttag
    23281 tgatcggtgc gatgccattc gtcccctctt ttaagctatt caaaaagctg tctgcgtcat
    23341 ttccaatagg tgaagtgact ccatagccag taattaccac tcgattcatt tgtttgctct
    23401 ccttttctta tccgtgcatg accaatccgc catcgacatt gatgacttgt ccggtgatat
    23461 atggattttg tgctaagaaa actgctgttt gtgcaatatc ttctacttgt ccgaaacgct
    23521 gcatcggaat ttgcttttcc gcttgttcct tgactttgtc tgctaaaact tccgtcatat
    23581 cagttgtgat gaatcctggc gcaatggcat tacatgtaat gcctctagtt gcagcttctc
    23641 gtgccacaga ttttgttaat cctactacgc cagctttact agctgcataa tttgcttgac
    23701 caatatttcc aatcaaccca gatacactag agagattaat gatggcccct tctcgctgct
    23761 tcatcatttt tttcaatacg tgctgcgtca tattgaaggt tccaatcaaa ttgatatcca
    23821 gacatttttt gaaatcttct gcatccattc gcatcacaag tttgtcattt gtgattccgg
    23881 cattgtttac taacacatgg atcgatccaa gcttttcttc tgcttctttg atcatttgtc
    23941 ccgctttctc ataatcagag atgtcaccgg aaactcctac gcattttaca ccgaatgctt
    24001 cgatttcttc tatcttttct tttggaatct ctccgcgacc attcaaaatg atatttgctc
    24061 cagcttttgc aaaagctaag gccatcgctt tgccgattcc tcttgtacta cctgtgataa
    24121 atacattttt tccttttaca tccataacgt tcctccttat agtattgcta ttgtttcatc
    24181 aaatgtctgc ttattctcaa cacgcagaac tggtattgtt ttatcgattt ttttcataaa
    24241 tccgcttaat acttttccgg ggccgacttc gatgacttgt tcgatgccga ttgtttttaa
    24301 cttatggata ctctcataga aacgaacagg ttgcatgact tgctgttcaa gtagtcccgc
    24361 gattgtttct ttcttcatta tttccgttgt tgtgttgctt atgataggaa aagaaggttc
    24421 agagaattga atctgactta ggtccttcgc cagttttttt gctgccggtt ctaaaatagc
    24481 tgtatgaaaa ggcccgctga catttagagg gatcattctt ttgaaccctt tttcttttaa
    24541 tagtaaaaca gcttcgtcta ctgctttttc ttcgccacca atcacgatct gctgaggtgt
    24601 attataattt gcaggagata cgatgccgta cttgctagct tcatgacagc tttcctcgat
    24661 cgtttcaatc ggcgcattca tcactgcaac cattttcccg cttccagctg gggcagcttc
    24721 tgtcatataa gcgcctcttt ttgctactaa cgcaactgct tcagtgaagg atagcgcatt
    24781 actagctact aatgccgaat attctcctaa actcagtcct aaagctgcat ctgggatgat
    24841 tccatgctcc ttcaataagc gataatacgc gatgctgact gttagaatag ctggctgtgt
    24901 atattgcgtc tgatccaaac gttcattttc tgtaaagcaa agttcagcca tatcgtagcc
    24961 gagaatctca cttgcttcat caaatgtctg ctttacaacg gcttcttcat ataaatcttt
    25021 tcccatacct tgatattgtg caccttggcc actaaataag aatgctgttt tcatgttttt
    25081 ctccaatata tccagaatat atgtttgatt cttcctattc aaaaaggccg agacattcct
    25141 atttcaggaa atgtcccagc ccccaaccta attttaatga ttatgcagtt tgtttttcta
    25201 cgtaagtcac taaatcttga accgttttga ttccgtcttc tgtttcgatt ttcacgtcaa
    25261 attcatcttc aatttcattg atgatttgga ataaatccaa gctgtcagct tctaagtctt
    25321 cttggatgtc agtagtcaat ttgatttctt cagtttcttt tcctaattct tctgcgatga
    25381 tttcttgaat tttttcgaat accataataa tatattcctc cgatattgtt tgctttttat
    25441 ttttataatt taacagtcta caactccatg agaatacttc cccatgttaa acctccgcca
    25501 tagcctgtaa atattacttt ctgctgtttt cctaaggtga tctttccact agaaactgct
    25561 tcatccaata agatagggat gcttgcagca gatgtattcc catatttatc catattggtc
    25621 aggaactttt cttgcggcat ctttactttt cgggcaattt tctcaataat tctgaggttt
    25681 gcctgatgca aaagcagata atcaactggt ttgtctttca tgacttcttg gatattgttt
    25741 gcaacgtctc gtacggcaaa atcaaaaata tctctgccag tcatttgcaa ataatacgaa
    25801 ccttctgaat cttctgaata gaacggattt ttattttgct ggtagccagc ggtcaatgag
    25861 ctacttctgg taccgtcagc tgctaatttt tctttcagca attttttctg tggattcgct
    25921 tctaagagaa tcccacctgc tccatcgcca aacaacacag cagtcgtacg atcagaccaa
    25981 tcgattagct tagatagtgt ttcaccacca atcaccagtc ccttagttga gccgcagcgg
    26041 atcatttttt cagccatgct taacgcataa acaaatcctg agcacgcagc actgatatca
    26101 aaagcgatag catgagttgc tcccaatttc ccttgtactt gtgctgctac agatggtgtc
    26161 gtgtaatcgg gcgacattgt tgctactaaa ataaaatcca aatctttagg gtttagattt
    26221 ttctgtttta tcaatttttc cgccactaaa atgcataaat cagaagtctc ctgatttgtt
    26281 acacatcttc gttcacgaat acccgtcctt gacgaaatcc attcatcaga ggtatccatc
    26341 atcttggaaa gatcattatt agtaataatc ttttcaggaa ggacgctggc tgttgcagta
    26401 atcattccaa attgttccat taaatatcct cacttatagt cttgcaagaa gtcgtgcaag
    26461 tttttcagtg catccagcaa ggcatgctgt tcctccgtag ccattccgtc taagatgttc
    26521 ttgaccattt cacgatgaaa atgctgatgg acccgaaata gtaactttcc ttttttcgtt
    26581 agtcccaact ttacaacgcg gcggtcatct tcactgcgga tacgttcgac atagcctttt
    26641 tttactaact tgttaatagc agtggtcaag gtcccaacag taattgatag ttctttggct
    26701 acttctgaag ttgttttttt cttgtacatc ccgattgcct cgatcgtgtg catctctgtg
    26761 attgacagat ctttgaattg agattttttc agctcagact cttcgattgt caaaatatca
    26821 ttgaagacac taacaagata atcatttact gtttttaaat ctggttccat gttccaccgt
    26881 ccactaatta gtttgattat caaatctttt gatgttcaaa ctatatctta acttttttca
    26941 aaatgcaacc ttaaaagatc atttttttac tatatgagct ttttttaaga ttttactcat
    27001 ttaaaacaac tgataaatca gcatttataa tttcttttat tagaaaaatc atctatctca
    27061 catattttga ttatcaaagt attttctgtg aacagaacta tagaattccc taaaacttgt
    27121 ccattatttc aaaatttgtt atagtctttt tattcaaagc gtaaaaggag atcctatgtt
    27181 aaaagaaaaa atgaaaactc tccccatgct gccgggtgtc tatcttatga aagaccgaga
    27241 tggtaaggtc atctatgttg gtaaagcaaa acggctcaaa aatagagtaa gcagctattt
    27301 tcatcagaat aaacagcatt caaaaaaagt attacgtatg attcatcata taacagattt
    27361 tgatttcgtc gtagtagaca cggaattaga tgctttattg ctcgagtgtc aattgatcca
    27421 acattaccgt cctttttata accgacaaat gaattatttc agtaattata attatgtaca
    27481 tattaccaat aagggatttg tattaacaga tactcctacg gctcgaacat acgggccttt
    27541 tcgtctctat aaaaaaatgc cgtctatttt gcgtataatg gaagaaactt atcaaatgcc
    27601 ttggttgtct gaaattagtt tgttagcttt gcgagtccag cttcctgacc tacaggaaat
    27661 gacttttgaa cagaaaaaaa aggaactcca agggctgttt caaggccgaa ataaaaagct
    27721 actgacttac ttgaaaaaaa gacaacaaca tttcatctat cagttgaatt tcgaaaaagc
    27781 cggtatgctt caaaaagata tcgaattagt tacttatttt attagacgga tccaggaaca
    27841 aaagcagttt ttaagaacac catcgctcac cttcagcatg ccattagcag cagatgagag
    27901 tcagaaaaag cactacctcg tttgttatgg tcaacttgca gaaacaatga ttacctctgg
    27961 tgacgtttct cctgacttct attatgaaaa gaaagaagct catttatctt tgaaacgtca
    28021 attgtcaaaa gaagaaatcg atcctgtaca aatcttgatc agttaccgca aaaaattaga
    28081 aaaagaacag tttgaaattg aactgttaaa caaaaaagaa gctgaaaagc aactaaatta
    28141 gttgcttctc ggcttctttt aggcatcttc aacttcaaat gtcaattatc ctaaaattta
    28201 ttggattaag tcatagattt ccatcgctac gatatcgata ttatcaaatt cgtattgctg
    28261 atgagtatct gtttcagtta attcaaatgt atcggtcgct ttatcataag ttactgtaca
    28321 cttctctacg ccttcttttt caaaacggcg aacttgcagt tcattatctg ctgactcgac
    28381 catagcctcc agtcgtttta tgattgcgac taattgtgaa tgtgtcatcg gaggtcctcc
    28441 ttttcttatt tctgttatat tgtatcaaaa gcatagccta cttgcattga ttttctctaa
    28501 aaaaactcgt tttttatgat tttttttctg gtatttttcc ttcaggccac caaagtttga
    28561 tcaaagcttg ggttccgtca ttctcaaaac ctttatctgc caactcttca tatagtttca
    28621 atgcttgggt agttgctggc aatacaagat ccattttttc tgcttcttct aatgcaatct
    28681 ttaaatcttt gataaaatgt ttgacaaaaa aaccaggtgt gtaatcttct tttaaaatac
    28741 gaggagaata attgcttaaa gaccagttag ctgcacttcc acctgaaagc gtctcaatca
    28801 ctttctttaa atcaagtccg tttttttgtg catagacaag catttccgtc attcctgtca
    28861 tcgtacctgc aatcatgatc tgattagcca tttttgtatg ctgacctttc ccagcagaac
    28921 catgcaatgt aaatgtcgta ccaaattctt tgaataaagg cagtacttga tcataaacgg
    28981 cttcttctcc accgaccatg atcgtcagtg taccattctt tgctcctaag tcaccaccgg
    29041 ataccggtgc atctaacgca tctgcgcctt tttcttttgc tgtttttgca attttttctg
    29101 ccaggcttgg tgtacttgta gtcaaatcaa ctaaaatatg tcctgagata tctgcagaaa
    29161 aaatccccga ctgtccatag tagatttctt ctacatcttt ggggtaacct accattgtaa
    29221 agagaatatt actttgttct gctaccgctt ttggtgtatc tgcccagata gctccttctg
    29281 ttactaaagg atcagctttt gcttttgtcc gattatagac agctacttcg tgtcctgctt
    29341 ctagtagatg gcgtgcgaca gcacttccca tgacgcctgt tccgataaaa ccaagtttca
    29401 ttttttcgac ctcctaaaaa tcaaaaatcc ttgcttctat gctagtatac gctagaaaca
    29461 aggatttttt cataaaaaat gtttgaattt tatcttttaa atgaatttaa ttttattttg
    29521 attcgttcaa tcgagtcttc ttcttctttt cttcgcatct cttcttcatg ataaacaacc
    29581 atgtcttgat aaaattcgat catagcttcg ccgaaatgtg tagatgaaat atcataaagt
    29641 ttagcttcta aaacttttgg atctttagga acttcactgg aaatataatc aataagtgct
    29701 ggtgcaaagt cttcgtcggt ttcaaaagtt ttccctaatg aaggatgatc gaacaatcgg
    29761 ttcaaataag catttccttc cacgacacaa ggtgttccgg ctgccatcgc ttctgtataa
    29821 gtaagccctt gtgtttctga agtagaagca ctgacaaaat aatctgccgc tttataataa
    29881 agtgctacat cattgttgtc cacttctcct gtgaactgca caaattcttc taactgcagt
    29941 tctgagatca attcctctaa ctcttctaca taaggtcctt ttcctacgat aactaatctg
    30001 gcgttaggat aagcggagat gacctgagga aacccattaa tcaaggcttg gatatttttc
    30061 tcataagaaa tcctgcttaa agaaagaatc atcaaatggt tttcctgaag acctagtgac
    30121 tcccttaatt tgctgatatc ctgctgagtg atatcagaac gtttgaattt ttcaatgtcg
    30181 ataccagtag gaatcacgcg aagcggtgct attacaccat agctgcgtaa tgtatcaata
    30241 acacgttcac ttggacaaac tacacctgtc gtatgatttg caaataatct agacaagtat
    30301 tttacatggg attgcctgat cacttttcct ttaccgatat aatgaaggta gtcttcatac
    30361 atcgtatggt aagtatggat cagaggtatt ttcaacttct tagctaccat cttcccaagt
    30421 atccctgctc cgaattctgt atgggtatga ataagatcta gttctagttc tttggcaacc
    30481 aaataagcat accacatgcc tctaacaaca actcttcgat ccttgaaaga aataaatggt
    30541 acacttggca ttcgaatcac atctttttcc aacttatccg cattaggatc ggtcgttgta
    30601 aaaatataga cttcatgccc atgtctttct aattcttgtt ttaatgtttt gatcgatgta
    30661 gcaactccgc tgacttgcgg aaagtaggta tcggtaaaaa agccgatttt catgtttttc
    30721 gcctccaaac tagtccttga cctgatttac tttttttaat ttgctagaga ctttttcatt
    30781 cagtacactt tcgtatacat ttttaagttc ttcaccgatt ttattgatac ttcgctcttt
    30841 tgctgtttga taaccggctt gtgtcgtact cggtagttct tgattggtaa tctgttggat
    30901 cagtcggata aattcttcgt ttgtcttccc catatagcag ttcttgctat cttctaacca
    30961 gccattatac actggaatat ctcgaacaag tacgttttgt ttgcttgcta aagcttctaa
    31021 aacaacgatc ccttctgtct cttcataaga agggaaaaag aatagatctg cacctgaata
    31081 cgcaccttct atgatctctc cacgaatgta accaggaaac aggacatttt caggatgatc
    31141 ttcttttaca attttccgga tgttccgagg aatcgagtac atgggaacat gaccaaacca
    31201 aataaatgtg tattcgggca tcttcttagc aatttctacg aaatcaatga ttcctttacg
    31261 ttcgaaaaac aaaccgacac aaatgattac tttttgatcc ggagacagtt tgaaatattc
    31321 tctgaacttt tgttctttca ttggatcagg gtgatatttc tctaagtcga tcccattgga
    31381 aatagcttga atcggcacac ggataccata gctttccaac aactgttttg aataaggtgt
    31441 tggcgtgatc agatagtccg ctttttggta caatcctacc agatattttt ttacgatagg
    31501 agaaagctgg ttcgatccga taaaggaatt tcggaaatct tcctctgtag aatgcgcatg
    31561 atagacaact ttttttcctt ctcttctggc tttattgacc atattatgac tattgattcc
    31621 gtatgtattg atgtgcagga tgtcataatc ttctttttca tctagtgtat aagagatacc
    31681 gacttccgtc agagcacgtt tttgatgatc aagcgcacgc ccgattccag attttgcaag
    31741 catcttttca ctttcaaaat ataataatac cttcacctta cgtccccctt cacttatata
    31801 gtataacata gcctacaaac tgtcactcaa acttaagccc tattttt
//
LOCUS       DACDMM010000015        31606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDMM010000015 DACDMM010000000
VERSION     DACDMM010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.015"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            155..1711
                     /gene="rny"
                     /locus_tag="IWD16_000533"
     CDS             155..1711
                     /gene="rny"
                     /locus_tag="IWD16_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP9087617.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1790..2596)
                     /gene="proC"
                     /locus_tag="IWD16_000534"
     CDS             complement(1790..2596)
                     /gene="proC"
                     /locus_tag="IWD16_000534"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP9087618.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            2737..5310
                     /gene="mprF"
                     /locus_tag="IWD16_000535"
     CDS             2737..5310
                     /gene="mprF"
                     /locus_tag="IWD16_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9087619.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            5464..6315
                     /locus_tag="IWD16_000536"
     CDS             5464..6315
                     /locus_tag="IWD16_000536"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP9087620.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            6560..7498
                     /locus_tag="IWD16_000537"
     CDS             6560..7498
                     /locus_tag="IWD16_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP9087621.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            7521..8201
                     /locus_tag="IWD16_000538"
     CDS             7521..8201
                     /locus_tag="IWD16_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9087622.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            8174..8998
                     /locus_tag="IWD16_000539"
     CDS             8174..8998
                     /locus_tag="IWD16_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9087623.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            9165..9983
                     /gene="purR"
                     /locus_tag="IWD16_000540"
     CDS             9165..9983
                     /gene="purR"
                     /locus_tag="IWD16_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP9087624.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            10486..11859
                     /gene="glmU"
                     /locus_tag="IWD16_000541"
     CDS             10486..11859
                     /gene="glmU"
                     /locus_tag="IWD16_000541"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP9087625.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            11953..12924
                     /locus_tag="IWD16_000542"
     CDS             11953..12924
                     /locus_tag="IWD16_000542"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9087626.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      12930..13032
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13098..13667
                     /gene="thiT"
                     /locus_tag="IWD16_000543"
     CDS             13098..13667
                     /gene="thiT"
                     /locus_tag="IWD16_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP9087627.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(13748..14521)
                     /locus_tag="IWD16_000544"
     CDS             complement(13748..14521)
                     /locus_tag="IWD16_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087628.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            14788..16488
                     /locus_tag="IWD16_000545"
     CDS             14788..16488
                     /locus_tag="IWD16_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP9087629.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            16458..16676
                     /locus_tag="IWD16_000546"
     CDS             16458..16676
                     /locus_tag="IWD16_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087630.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            16696..17586
                     /locus_tag="IWD16_000547"
     CDS             16696..17586
                     /locus_tag="IWD16_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP9087631.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            17604..17888
                     /locus_tag="IWD16_000548"
     CDS             17604..17888
                     /locus_tag="IWD16_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087632.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            18375..18989
                     /gene="gmk"
                     /locus_tag="IWD16_000549"
     CDS             18375..18989
                     /gene="gmk"
                     /locus_tag="IWD16_000549"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP9087633.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            18994..19299
                     /locus_tag="IWD16_000550"
     CDS             18994..19299
                     /locus_tag="IWD16_000550"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9087634.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            19552..21960
                     /gene="priA"
                     /locus_tag="IWD16_000551"
     CDS             19552..21960
                     /gene="priA"
                     /locus_tag="IWD16_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP9087635.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            21975..22466
                     /gene="def"
                     /locus_tag="IWD16_000552"
     CDS             21975..22466
                     /gene="def"
                     /locus_tag="IWD16_000552"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9087636.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            22459..23397
                     /locus_tag="IWD16_000553"
     CDS             22459..23397
                     /locus_tag="IWD16_000553"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP9087637.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            23397..24755
                     /gene="rsmB"
                     /locus_tag="IWD16_000554"
     CDS             23397..24755
                     /gene="rsmB"
                     /locus_tag="IWD16_000554"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP9087638.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            24768..25508
                     /locus_tag="IWD16_000555"
     CDS             24768..25508
                     /locus_tag="IWD16_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP9087639.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            25505..27574
                     /gene="pknB"
                     /locus_tag="IWD16_000556"
     CDS             25505..27574
                     /gene="pknB"
                     /locus_tag="IWD16_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP9087640.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            27739..28638
                     /gene="rsgA"
                     /locus_tag="IWD16_000557"
     CDS             27739..28638
                     /gene="rsgA"
                     /locus_tag="IWD16_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP9087641.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            28651..29301
                     /locus_tag="IWD16_000558"
     CDS             28651..29301
                     /locus_tag="IWD16_000558"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9087642.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            29302..29940
                     /locus_tag="IWD16_000559"
     CDS             29302..29940
                     /locus_tag="IWD16_000559"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP9087643.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            30095..30949
                     /gene="mreC"
                     /locus_tag="IWD16_000560"
     CDS             30095..30949
                     /gene="mreC"
                     /locus_tag="IWD16_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP9087644.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            30953..31462
                     /gene="mreD"
                     /locus_tag="IWD16_000561"
     CDS             30953..31462
                     /gene="mreD"
                     /locus_tag="IWD16_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP9087645.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
BASE COUNT        10337 a         5593 c         6882 g         8794 t
ORIGIN      
        1 aaagttgaca ctacggggta caaacattag aataaagttg tgcatgtttt ttgtacacat
       61 atatgcgaag atgcatattg acgagtttag aataatttat catagaagct gtatagatga
      121 taaaaatgaa aaagatagta tggaggtgga ttttatggta ttcaacattc tcctcgctat
      181 catcggttta attgtcggtc ttggtttagg gtttatggtc gcaaagtcac gtcacgagaa
      241 agaaatagct ggtgcacaat catcagctgc tggaatcatc gatagtgcaa aaaaagaagc
      301 agaaactcta aaaaaagaag cattgttaga agctaaggaa gaaaatcaga aataccgttc
      361 tgaagttgaa agtgagctga gagaaagtaa gttagaatta aaatctcaag agaatcgttt
      421 gattcaacgc gaacaaacgc tgaatcgtaa agatgactcg ttagaaaaac gcgagaactc
      481 acttgaaagt aaagaagaag cacttagttc aaaacagcaa ttaattgaag agcgagaaaa
      541 agacgttgaa aaaatgattg aagatcaaca aaaagaacta gaaaaaattg ctggtttatc
      601 aaaagaggac gcacgagatg tcatcatgaa atcgactgaa gaagagctga atcatgagtt
      661 aactctaatg gtaaaagaat ctgaacagcg tgccaaagaa gaagcagatc gcaaagcgaa
      721 aaacttatta tcattagcga ttcaacgctg cgcagcagat caagtttcag aaacgactgt
      781 ttcagtcgtt acccttccga atgatgaaat gaaaggtcgt atcattggac gagaaggtcg
      841 taatattcga acgcttgaga cgctgactgg tatcgattta atcatcgatg atacaccgga
      901 agcagttgtt ctttccggat tcgatccaat ccgaagagaa atcgcacgga tgaccttgga
      961 aaaactgata caagatggac gaatccatcc agcgcgtatc gaagaaatgg tcgagaaatc
     1021 tcgtaaagaa atggatgaac gtattcgcga atacggggaa gaggcagcgt ttgaagtcgg
     1081 tgctcataca cttcatccag atctaatcaa gatcatcgga cgtctgcgat tcagaactag
     1141 ttatggacaa aacgtcttga agcattcggt cgaggttgca aaattagctg gtatcttagc
     1201 agcagagctt ggcgaagata tccaattagc aaaacgtgcc gggttgctcc atgatattgg
     1261 gaaagcactt gaccatgaga ttgaaggatc tcacgttgag atcggtgcgg aattagctgc
     1321 caagtacaaa gaaaacccag tagtaatcaa cgcgatcgct tctcatcatg gagatgtgga
     1381 agctacttct gttatctctg tccttgtagc tgcagcagat gcgctctcag cagcaagacc
     1441 tggtgcgcga agtgaatcat tagaaaacta catccgtcgt ttaagaagtc tggaaaatat
     1501 ttcgaacagc ttcgccggtg tagaatcaag ttacgcagtt caagctggac gcgaagttcg
     1561 ggtaatggtc aagccagaag aaatctcgga tttagacgct gttcgtttag tacgtgatat
     1621 tcgtaaacga atcgaagaag aactggatta tcctggacac atcaaagtaa tcgttattcg
     1681 ggaaactcgt gcaactgatt atgctaaata ataaaaaata caaaagaaga gccgcggcaa
     1741 aagccacagc tcttcttttt ttgtaaggca cgaaaaaaca ttgctatcat tattttttat
     1801 tcaacatctc aagatctttc tcgatcgtcg tattgattcc tttgatgatt gctgggatga
     1861 atcctgcttc ttctaaggca actacacctg caacagttgt accgccagga gaagaaactt
     1921 ggtctacaag ttcccatgga tttttatcac ttgaaaggaa tttttgacaa gaacccataa
     1981 ttgcttttgc agcaatctca gtggctgtct gtttggacaa accattcagt acaccagtcc
     2041 gtcccatcgc atcgatcagc atgtaaacga atgcgggtga gctgccagca aggccaatga
     2101 atgtactgaa atctgattct gctaaaggat agacgctgcc gattgcttca aaaatcatcg
     2161 tagcttgctg aagctgttcc tccgtggcag cttcattttt gcaaatcgca gaaacgcctg
     2221 ctcctacgct gacattgaca ttcggcatca cacgtacgat ggaggcctga ggatcttcaa
     2281 gcgctgtttc taaatctatt aatgattttc ctgaagcaat agaaatgaat aagactttct
     2341 ggctatattc tcttagttct tccaatactt tttcaatgat ttttggtttc acggctaaaa
     2401 gaacgatatc tgattgtgat gcaacttcct gattactttt gcatgcatgg acgccagttt
     2461 cagcagcaaa tttttgtgca ttttcaaaat gtccactgga aacgaacacc tcttctttcg
     2521 agactgttcc tttttttaat aaaccatcaa taatagcttg tgccatattc cctgtgccga
     2581 taaatcctac tttcatgtta tcctctcctt tagcattagg atatctttct ttttctatca
     2641 tcgcaagcaa agaacaggtc taaagtggaa aaattaaaat aaataagata acattggggt
     2701 atgacacaaa agtatacaag ttaggaatgg gaaaatgtga aaaataaacg atacttgatg
     2761 ctgcaatgga taaaggaaca tagtctgatt ttaaaactga tttttttcgg ctccgtgtta
     2821 gtctttgtgg caaatcaagt cgcaaatatt gctaatggca tgagttggca agatatttgg
     2881 caaaggatgg gacaacaaag ccgcatgacg atcctgctca tgattctggc tggactgctg
     2941 ggtgtcactc cgatgttgtt gtatgattgg gtgacgatca gagtcttaga aaaacaagga
     3001 aaaccaaaaa tggaaagaaa agagtttctt cttgctgcgt gggtaacaaa cacgatcaac
     3061 aatctcgccg gttttggtgg tgtggtagga gcaagtctgc gggctagttt ttatggaaag
     3121 cagacaaata gaaaaatggt tttagccact gtatcaaaag tcgcactatt tatgctgact
     3181 ggtttgtctg tatggtcgtt tcttacattt attgatgtct tttttattca gtcagaaagt
     3241 atttttcgaa gctattggat ctggcttttc ggcggtagtt ttttagcacc agtcattttc
     3301 ttttttgctt atctaaagag aaagacattg ttcttcgagt tttatccaaa aggcttgctt
     3361 ggtctgctac tggcatcctt tggacaatgg acgggagccc ttgcggtatt tttgatcatc
     3421 ggcaagttga tgagaataga tatttcgatg ctttcgatct atcctatgtt catgattgct
     3481 acattgatag gaatgctgac gatggtccca ggtggaatgg ggacttttga cgttttgatg
     3541 atcttaggaa tgagtcaatt gtctgttcgg caagatgtag cagtagtttg gctattatat
     3601 tatcggttgt tttattatgt tctacctttt atgacgggac ttctattgtt catcacacat
     3661 acaggagtca aaatcaatcg atttttagat aacattccgc gactgttttt ccaaaaggtt
     3721 gcgcatatcg tgctcgtaag tgctgtctat tttgcgggga tcatgatggt acttctatca
     3781 acaatcacta atctgtctgc aatcagtcgg ctatttcaag tattattgcc gttctcattc
     3841 aattttttag atcagacagt gaatctattg gttggctttt tattactcgg ccttgctaga
     3901 gcactttcag taaaagtcaa aaaggcattt ctgcctacga ttgctttact atcttttggt
     3961 attttaaata caatcacacg tacgttatct tggcagttga tattggtata tgtcttgatt
     4021 ctgttagctg tgtggttatc cagaaaagaa ttttatcgaa caaaattcgt ttattcttgg
     4081 ggagctataa tctttgatgc tagcttgttc ggctttttgt ttatcgttta cgcaattgca
     4141 ggctatcaca gcggacgctt tttagtcggt cgaataacag gaaatcattt tattttgttt
     4201 ccttcagatg atgtgtggtt ctcagggctt attggtttgg gcatctcatt gctagcttta
     4261 gtggcgcttt atcaatattt agctgaagat actacgaagc ttggcagtga gtacgaaaaa
     4321 gaccgttttg accgtttatt ggaaacatac ggaggcacag ctacaagcca tcagcttcgc
     4381 ttgtcaggtt acagttattt ttattaccaa gaagacgacc aagatcaagt cgtttttggt
     4441 tatcagatca aaggaaacaa atgctttgtt ttaggagatc cagttggcaa tcctaaaatg
     4501 atgcgggcag caacgaatca gtttatgaaa caagcagatt gtttaggcta tcagcttgcg
     4561 ttttataaga tatcagaaag ctacgtagtg atgcttcatg aattgggttt tcacttcacg
     4621 aaagtaggtg aagcagggat tgttgatcta gcaacagaag acttgtctcg gctgagtcag
     4681 caacttgaat atcaacgttt gatgaaagaa ggataccatt ttacttggta cgatcaactg
     4741 ccggaagaat tacatcaagc tgtaaatgaa gtttcacaaa aatggcttca aggtgcacga
     4801 gaaaaatact tctctgtggg acgatttgat aaatcatatg tattatctag cggtgtcggt
     4861 attgtccgta aagatcaaaa agtcgttggc ttcatcaccg ctcagccgat gacgaagaag
     4921 caggcgggat atgatttgtt gcgggtctta cctaaagaat ctgaggaatt agctgactat
     4981 ctgttagcca atctgtttat tgtatacgag aaaatgggct actcggaagc caatctaggc
     5041 ttagctccat tagctaatgt tggagaaaca gatttttctt tctttcaaga aaaagtcatg
     5101 catatcgtat ataattatgg taattttttc tattccttcc agtctgctta tgaaaagaaa
     5161 ttaaaatatg tcgatcattg ggaaggacgt tattttgctt ataggaaagg aagtaatttc
     5221 ggatttgcta ccatgcagct actcagtctg ataggaaaag aaaaaggaaa tgttccgagt
     5281 ttggcagaag aagtgctcac agaattataa gaatcactag aacttgtttg gattatgaag
     5341 agaaaattct gtgaaagctg ttgaaacgat gacaaaatca accactaggc agacataagc
     5401 tatactttta tttttcccta tgatagaata cttgcgactt agacttttcg aatgggtgag
     5461 aggatggaat tgattgaaaa agcgccagcc aagataaatt taggactgga tgtattatat
     5521 agacggcaag atgggtatca tgagttggaa atggtgatgt cgagcgtaga cttggcagat
     5581 catctatttt ttgaagaaat aacagaagac aaaatcattg tagaaacaaa taaagcattt
     5641 ttaccggtag atgaacggaa caatgtttat caagctgctt tattgctaaa aaataaatac
     5701 aatatcaaaa ccggtgtcaa gatctttgtt aagaagaata ttccagtagc agctggatta
     5761 ggcggaggaa gtacagactg tgcagctgct ctaagaggga tcaaccaatt atggaatctg
     5821 ggctgttcgt tagaagaact ggcagaaatc ggtttggaag tcggtacaga tgttccttac
     5881 tgtgtctacg gaagtacggc atttgtcggt ggcaaagggg aagaaatcca agtattacct
     5941 tctatgccac agtgctgggt tgtgttggtc aaaccaagaa tgagtgtctc tacacgtaca
     6001 gtgtttagag aagtagatac gcaagccatc caacatcaag acatccacgg acttgctgaa
     6061 gccatccgag agcaagatta tcagcagatg atcaaaaaaa tgggaaacag tttagaagat
     6121 ataacgggaa agcggcatcc agtcatccat caaatcaaag agcgtatgat caagtacgga
     6181 gcagatgctg cattgatgag tggaagcgga ccaaccgttt ttgccttgtg tcaacattat
     6241 tcaagagcgc agagagtata taatgggtta aaaggttttt gtgaggaagt ttatttagta
     6301 cgaaccctgc aataaaatat aagaagtaaa ataggtgatg aaaaactgag gaatcctaaa
     6361 atggtattct tcagtttttt attgtagtga aatggctaga tatgagcagc aactcatcca
     6421 ttttacgatt taatcaagaa tatacatgct taaatcagaa attgatggat ttctattgac
     6481 tgtgtttttt tattttgata cattagttca cgttaataaa tcgtaaaatt ccgatttgtt
     6541 cattgaaagg agcatataaa tgaaaaaata tcttttgaca gctacggtaa tgataggtgc
     6601 tttactgttt gcagcatgtg gaaatacgaa caaggaagca gacaaaaaag aggatttgac
     6661 gatcgttacg actttttatc cgatgtatga ttttacaaaa gagatcgtag gggatgaagg
     6721 aaacgtgaaa cttttgatcc ctgctggaac ggagccgcat gatttcgaac caagtgcaaa
     6781 agaaagagca gaaatttccg atgcagatgt tttcgtgtat aatagttcag acatggaatt
     6841 ttttgtggat tcgttaaagg attcagtgga tagcaaacag accttgatga tcgaagcagc
     6901 taaaggaatc gaccgtttag aatcgcaaga agcagacgaa catgaagaat cagaagaagg
     6961 gcatggtcat tcacatgaat acgatccaca tgtttggctc gatccggtat tagctataaa
     7021 agaagtcaga acgattgctg aagaattagg cgaaaaatat ccagataaaa aggaaacttt
     7081 tacgaagaat gccgatgctt atatcaaaaa gcttgaagca ctggatcaaa aatactccga
     7141 tgagctaaaa gatgcaacaa accgcacatt tgtcactcag catgctgctt ttgcttattt
     7201 agcgaatcaa tacgacttaa aacaagtagc gatttcgggc gtttcaccag atcaggaacc
     7261 cacaccaagt cgcttagctg agctaaaaga atttgtgaag aaaaacaaca tcaaagtgat
     7321 ttattttgaa gaaaatgcct cttcgaaggt agctgagaca ttgtcgaatg aaacaggagt
     7381 gaagctagaa gtgttgaatc ctttagaaag cttgactaat gaacaaatca aagcaggcga
     7441 aaattatatt tctgtcatgg aaaaaaatct gaaagcttta aaagaaagta tcaattaagt
     7501 tttagaatgg aggttgtaag gtgcgttata tcgaaatatc tgatttgacc ttttactatg
     7561 atgatgaacc tgtactagaa gatgtctctt atcatgtaga tgcgggtgaa tttgtcattt
     7621 taactggaga aaatggagca gccaagtcaa ccttggtgaa agcatcgtta ggattactga
     7681 aaccttcaaa aggaatgata aagattgctg agaaaaatca agaaggaaaa aaattaagta
     7741 tcggttatat tcctcaacaa gttgcatctt tcaatgcagg ttttcctagt acggtgatcg
     7801 aattagtccg ttcaggaaga tatccaagag gccgctggtt caagcgtctc tctgaaaaag
     7861 atcatcaaca tgtacagcgt gctttagaag ctgtagggat gtgggatatg cgaaatcggc
     7921 gaataggtga attgtctggc ggacaaaagc agcgaatcag tttagcgcgt atttttgcta
     7981 ctgaccctga cttatttgtt cttgatgaac caacaacagg tatggatgaa gaatccagaa
     8041 atgaatttta tcgtcttctc cgacatagtg cgcatgtcca tgggaaatca gttctaatga
     8101 tcacccatga tcatgaagat atcaaacagt atgcggacag acagatccga ctggtgagaa
     8161 aggaggattc tcaatggcgt tgctttcata tgaattcatg agaagagcgt ttcttgccgc
     8221 tatcttcatt gccggaatcg ccccgatgct aggtgtgttt ttagtgatca gaagacaatc
     8281 gctgatggct gatacgcttt ctcatgtctc tttagcaggg gtagcgttag gattcttttt
     8341 gaaccttaat cctacactta cgaccatgct agtcgttgta ttagcagctg tgatattgga
     8401 gtatctgcga acgatctacc gaacctattc agaaatctcc attgccattt taatggctgg
     8461 tggtttggcg ctagcgttag tattgatgaa tttgagcgga gggaattcag caacaagtat
     8521 ccaatcctat ctgtttggtt cgatcgtgac cattacatgg gagcaagtca tctttttagg
     8581 aatcttattc gttgccatcg gtatattgtt ctttttattc aaacggccga tgtatgttct
     8641 gacttttgat gaagataccg cccacgtgga tgggctaccc gttcatttga tgtcgatgat
     8701 gttcaatgtg attactggtg tggctattgc tgtgatgatc ccgattgccg gtgcactttt
     8761 gatatccgca atcatggttc ttcccgcagc aatcggtatg cgattaggca aaagcttcaa
     8821 tgtcgtgatt gctatcagtg tttgtgtagg atttctaggg atgctgtcag gactaactag
     8881 ctcgttttat ctagatacac ctccaggagc aacaatcaca ttagtcttta ttgctttgtt
     8941 cctgattgta aatatcatca aacgaatatt ggttgccttc agaagaaaaa ataactaaat
     9001 tataaagaaa agaagcctta taaaaacgaa aaatttttta taggcttctt tttattttaa
     9061 taaataccga actattttct tattttttca taaaatgctc gaatttttga agaaaaactt
     9121 atataatgta aaacgataaa atttcttaaa aggagttgga ataggtgaaa gtacgtcgca
     9181 gtgaacgtct agttgacatg acacagtatt tattgaatca cccgcatgaa ttgatttcct
     9241 taacaagctt tgccgaacgt tatgaatctg ctaaatcttc tatcagtgaa gatttgggaa
     9301 tcatcaagaa aacatttaaa gaacgagggt atggaacctt ggaaacaatc ccgggtgctg
     9361 caggtggtgt attgttcatc cctgaaattt catatgaaga ggcgaaaaaa tatattgaat
     9421 ctctagcaga acgtctatca gaacaagacc gtctgcttcc aggaggatat gtttatctct
     9481 ctgatttgtt aggagaacct gaactgctgc gtcaagttgg aagaatcatt gcttctaaat
     9541 accttggtga aaaaatcgat gctgtaatga ctgtagccac aaaaggcgta cctattgccc
     9601 aagctgtgtc ctattatctg aatgcgccat tcgttattgt acgccgggac tcaaaaataa
     9661 cagaaggatc aacagtaagt gtcaactatg tgtctggatc ttctgaaaga attgaaaaga
     9721 tggaactgtc taagagaagt ttgaaacgtg gttcacgtgt ccttgtagtg gatgatttca
     9781 tgaaaggcgg cggaacagtc aacggaatga agagcttgat tgaagaattc gaatcagaac
     9841 ttgttggcat cactgtattt gccgaatcaa aattcaacgg tcatcgcgcc atcgatgatt
     9901 atacgtcact gctttatgtc aaagatgtag atactcaaac gaaaaatatt acggttgtgc
     9961 caggaaatta tttttcagaa taagaggttg tgaaaaagcc gcttagtttc gagtagtaag
    10021 aaaaattttg tgaaaatggc tttccacatt ttttcataaa tttggcttaa taccgagaaa
    10081 ctggcttttg aacaccgttt atctagaggt tgtgaaatca gcgtcctgtc ctgagtatta
    10141 gctcaataaa cggaaaaatt gctcctcata ttttgccgtt tattgagcta ataccgaagg
    10201 tcagctgatt gaaaaacgtt taatgaagag gttgtaagcc taccgtttaa ctctaagtca
    10261 caagaaacga tttcaaaaaa cagcttttca tgttttatga gtagtgtgac ttgtgaccga
    10321 ggagttggca ggagagcacc ggttaccaaa tagataggcc cgttatggtc tatctatttt
    10381 tttatccaga aaacgctggg ataaaagaaa atcacttgtt ttgttgaaaa aatgggtata
    10441 ctgggataaa ctgaaatcag caaactatat ttaggagtga cgcagttgga cgcacgttat
    10501 gcaattattt tggcggcagg gaaaggtacc cgcatgaagt caaaattata taaagtttta
    10561 catcccgttt ctggtaaacc tatggtagaa catatcatta atcgggtcag tgaaacgaaa
    10621 ccagatgaag tgattacgat cgttggacac ggagcagaac aagttaaagc acaactagga
    10681 gaacgcagta aatatgcgtt acaagcagaa caattgggaa caggacatgc tgttttgcaa
    10741 gctgcttctt tcttggaagg aaaaaaagga acaacgttag ttatcagtgg cgatacacca
    10801 ttattgacta ctgaaacgct gaacaatctt tttgaatatc accaaggaaa aaatgcaagt
    10861 gcaacgatct tgacggcaca agcagaaaac ccaacaggat atggacgaat catccgtgac
    10921 catattggga tcgtagaaaa aatcgtggaa caaaaagatg caacaccaga agaagctctt
    10981 gtacaagaaa tcaatacagg aacttactgt tttgacaacg aagctttgtt cgatgcgtta
    11041 agcaaagttg gaacaaacaa tgctcaaggt gaatactatc taacagatat catcgaaatc
    11101 ttaaaagaag aaggccatac agttgctgct tatcaaacgg atgattttga agaatcaatg
    11161 ggagtcaatg atcggatcgc gttagctaaa gcaaatgaga tcatgagaaa gcgaattaac
    11221 cagatgcata tggtcaatgg cgtaagcttt gtcgattcag caacgacata catcgatgca
    11281 ggcgtagaaa tcggtccaga cacattgatc gaagcaggtg tacaaatcca aggaaataca
    11341 gtcattggtt ctgattgtgt catcgggtca cattcaaaaa tcgtagacag taggattgag
    11401 gatcacgtcg tcattgaaaa ctcagtcatt gaaagcagcc atgtcaagaa acatgcagat
    11461 gtgggacctt atgcacattt acgaccaaaa gctgaaattg gcgaaaacgt ccatatcgga
    11521 aactttgtgg aagtgaaaaa tgctcaaatc ggcaaaggaa ctaaagtcgg tcacttgact
    11581 tatgttggtg atgctacgct gggagaagaa atcaatgtcg gatgtggtgt tgtcttcgtc
    11641 aactatgatg gaaagaacaa acatcatacg actgtcggcg accacagctt tattggttct
    11701 agcacgaata tcatcggacc tgtcgaggta gctaaaaaca gttcgattgc tgccgggtca
    11761 acaattacag acaatattcc agaatatgcg ctggcaattg cgagagcaag acaagtcaat
    11821 aaagaaggat atgcaaaaaa actcccttat ctaaactaag acttttgcaa aatcacttga
    11881 tttatttaaa aaatatgact actatttatt aggtagaatt ctagagttgt gaaaaagaaa
    11941 gtggaggtcc ccatgtcaaa acattacttt gacccaagat taaaaatttt tgcgttaaat
    12001 tccaatcgcc cattagcaga gaaaattgca gcagcagtcg gcgtcgagtt aggaaaatca
    12061 tctgttactc aatttagtga tggagaaatt caagtaaaca ttgaagaaag tattcgtgga
    12121 tcgcatgtct atgttattca atcaacgagc agcccagtca atgataactt gatggagttg
    12181 ctgatcatga ttgacgcact gaaacgagca agtgcaaaaa caatcaacgt agtcatgcct
    12241 tattatggct atgcgcgtca agaccgtaaa gcacgtgcac gggaaccaat cacagcgaaa
    12301 ttagtagcaa acatgattca aaaagctggt gcaacaagaa tgctgacttt ggaccttcat
    12361 gcagtccaaa tccaaggttt ctttgatatt ccagtcgatc atttgatggg tgcgccactt
    12421 attgccaatt atttcttaga aaaaggaatc aaaggcgatg atgttgtcgt tgtttcacca
    12481 gaccatggtg gtgtcacacg ggcacgtaaa ttagcggaat ttttgaaatc tccgattgcg
    12541 atcatagaca aacgccgtcc aaaagcaaat gttgcagaag taatgaatat cattggacaa
    12601 gtcaaaggca aaacttgtgt actgatcgat gatatgatcg atacagcagg tacgatcaca
    12661 ttggctgcca atgctttgaa agaagctggt gcaactagtg tgtatgcttc atgtacacat
    12721 ccagtattat ccggccctgc tttacaaaga atagaagatt cagctatcga gcgtctagtt
    12781 gttacagact cgatctatct tccagaagat cgtaaaatcg acaaaatcga tgaagtaagt
    12841 gttggcggac taatgggaga tgcaatcaaa cgtatccatg aaaacaaacc agttagtcca
    12901 ttgtttgaaa caaaaaataa ataataaata aactcacaaa ggggagtcca attgggctga
    12961 gattgaatct atttctaaac ccttcgtacc tgtatcggtt atgcgagcgt aggaattgtg
    13021 aataaacagc tgtttgacta ttttcgaaag ctgcttcctc ctttgtgact tttttattca
    13081 aaggaggaag tttttttatg ggaaaaaata gagtttgggt agaaggaacg attgttgcag
    13141 cgttagcgat ggtgttgtcg ttgattccga tccagatcgg cagcagtttt tctatttcat
    13201 tagggcagat tccattgact ctttttgcgt taagaagagg gacaaaggca gggatgctgg
    13261 caggtttcat ttggggcatc ctacattttc cgttgggaca agtttattat ttaagtgtcg
    13321 ttcaagtgtt gattgagtac ccgattgcct atacttttgc aggcgcagca gggctcatgg
    13381 cttcaaatgt ccaaaaaggc tatgtagacg ataatacgaa acaaattaga aaaagcatcg
    13441 tgtttggtgc attgattggc gcactttcca gatatttttg gcattttatt gcaggagtgg
    13501 ttttctgggg agcgtatgca ctatggggga tgaatccttg gctcttttct ttcgtaatga
    13561 atggtgccag cgggttgagt acagctattg taacgatcat cgttttatta gggatccaac
    13621 aaacgactcc ttcgattttt gtgccagata aacgacagga gctgtaaata aaaaagaagc
    13681 aagaaacttg agacagaatc atgtcctagt ttcttgtttc ctttttattt tttgataaga
    13741 aatagatcta cttcaatggc agataagaaa gatcggcatc ttctaccaat tgccagtgtc
    13801 gatcggtgac agagtagatt aaagtagcga tgatttcctc ttctcgatac cgggtgcctt
    13861 ttaatttcca aatataatct tcaatcacat agattgggat acgatattta ccatcgatca
    13921 aaccaacttg atcaaaagaa aaaatgatcc cttcctgcat caaattacgc atttctttca
    13981 ataattccac actgattttt tcaacaggtg tttctttttt gaaaaaagat ttttttacat
    14041 tgatcttatc tgacaagatc gaacttaatt tactagcaaa aatcctataa aattcatcta
    14101 aatagcgata ataaatccga ttcagctgat cattaagatc gaagtataca aaattattct
    14161 gtagtttata aaaaaacggc gaatgaagat gtgttttcat atgaccaaaa tataataatt
    14221 ctgaaatttc taaaggtgtc aattctttca gcatcgttaa atcagtaaag tcgatccact
    14281 tcaattcatc gaatccacgc ctgttacgag aagaggaaaa gaaacgctga atattttctg
    14341 taccacggat tactttcagc cctgtatggg attcatattc tccagcagaa gcagatggat
    14401 caagcaagag caagttttga ggaaaatgca cgatactgtt tgcaaagtca gtagcagtca
    14461 gcccttttgt taaaacagca ttacttgtgg tatcaatatg tacataaact gactcagtca
    14521 tcatgttcct cctcttattt cctcttaatc tcttttattt tacaacattt gcattaaaaa
    14581 agcttgtttc ttttatttta aaattccatt acaaaatcac gttttcattg aaaacactct
    14641 cattttcaat taatttttgt gatttttgta tttttttatt taaaggaggt tttatgaatt
    14701 gttttcttat ggaatctaca aagtcgaata cgagactcat gaccttttta tgacgtactg
    14761 gtatacttga agaaaaagga gagcgcgatg gagataaaaa aagtttactt ttcacagaaa
    14821 atgtcgctgt attcgcctat ccagcaagca aaacaacccc ttccattttt tcaaagtcat
    14881 caagaaagta gagacgcttt ttggcgttct gcaaaaaagc aatgtgcttt taaagcagta
    14941 tcgaaaaaat ataccgtcta ttttactatt gctaaaagta gaggagatgt tatttttaat
    15001 aatctgttga ttgaaggtga aacttttgaa tggaaaaaat tttttcggta tatggaagct
    15061 tgcgctcgtt tttttattaa agaaaactgt tgttttttat ttcaatctcc tctatcagaa
    15121 gaatggttgc gtatttttca taaaaacggc tatcaaggaa ccacacaatt aaataaaaag
    15181 ctggtctatc atacagcact tgttttaggt ggaggaggag cgcacggtgc ttatcagatt
    15241 ggtgtgtggc aggcgctgaa ggaacatgat atcaactttg agattatcac aggtacctcg
    15301 gtaggtgcgt tgaacggcgc attgatttta caaggagata tgaaaaaagc tgtgaattta
    15361 tggaaaaagc tgtcgacaag acaagtgctt gcgttaccag aaatggctgc agttgaagac
    15421 cttcgtgaac gcttctatcg agaaagacgc caaatgacga aaacagcact aattgaaggc
    15481 ggggtttctt ctgctccatt ggaaaagatg gtcaaagaca atttggattt gtccttactc
    15541 aaacataatc ccaaaatacg tttatatacg gtgtctacta aactgccaga actagcggaa
    15601 gtagtgactg atatccaaca gacaaaaaca gaagaaatac cagattggat actcgcctct
    15661 gcttcatttt atcccgctat ggcttatcga aagatcggaa aaaataaata tgcagacggt
    15721 gggtatcgca acaaaatacc tattgatatt gcgattaaca aaggagcaac agaggcattt
    15781 gtcgtagatg tgcaagggcc aggcccagcg aaaaaaattc gtatgccaga catttttatc
    15841 cattggaaat gtcaaacatt gtggacacta ggcagctttc tgctttttga tagccaaagg
    15901 aaccaattaa atttacaatt aggctatctg gaaatgaaaa aacgattagg ctattactat
    15961 gggaactggt atacatttca ttctgttaaa cctgcaagaa catactggcg aggatttttg
    16021 tcttacttaa caaaagaaat tcagttagaa ttatcttttt tcaaaagcat aaaattttgg
    16081 caaaaactgc gtaatttata taagaacagg gtcgtaccag agacatgcgg attggcgatg
    16141 ctagaacttt tagcaaaaaa acatttttta ctgcccaatg aaatctatca agtagacatg
    16201 atgattcaaa tgatctgcca acaagatata aaaagtatgc cggatgattt attggcacaa
    16261 attggtcaat taagtactga agaatggcga aggtatcgaa aataccagca aaaaatacaa
    16321 aatgagcgca cgaaaactgt ttattttgat tatctgctac aaggaaaacg caaggatcgt
    16381 ttgcagcatg aattgcttaa gcaaccagtt gacacattac tgatcctata tttatattat
    16441 ctcaaggagg aacaaccatg gcacaagaat tttcatatga aatcgtagaa gaaatcgcta
    16501 tattgtcaga aaacaacaag gggtggcgta aagaactgaa cctagtcagc tggaatggtc
    16561 gcccgccaaa attcgatctt cgagattggg cgccagacca tgaaaaaatg gggaaaggcc
    16621 tgacattgac gaacgaagag tttgaacaac tgcaaaaagc aatagaaaat atgtaatgag
    16681 gaaggaccct gtcgaatgaa aagtatgact ggttttggca aagccatccg cgaaacaaaa
    16741 gcgtatcagt tagatattga aataaaaagt gtcaaccagc gttttctaga tgttcaaata
    16801 cgaagtccta aaatcctgaa ctttgttgaa aacgattttc gtcagcttat taaacagcag
    16861 ttaagccgcg gtcgaatcga agtatttgtg aatctagctt atattggcaa aaacgaaaaa
    16921 cagatcgtca tcaactggga attgattgat tcacttatca ctcaattgac agacggaatt
    16981 caacaacgat acggtagtca aaatcaagtg tcaattagtc ggatggcgga acgcttcgtg
    17041 ctggacgaaa acttcgtaac ggtagaagaa aaaccggaag agagcatgga agaattggca
    17101 cagttagctc tagatacatt gaaaagcgca cttgattcta ttgaggcgag tcgagaaaaa
    17161 gaaggtcaag ctttagcttt agtgttgaaa aagaatactg aagaattcaa agaagtacta
    17221 aaaaaactcg atagttttgt agaaatctat gaaaaggaat ttcaaataag gtatcaaaaa
    17281 aagctggagg aatttcttgg agcgaaagtc gatgagcaac ggttgttgac ggagctagcc
    17341 atcttgttgg aacgcggaga tattcacgag gaactggatc gtctggcgat ccatgtacaa
    17401 aaactagatg aacttatcga agcacaatgt cctgtaggtc gggaattaga tttcctgatc
    17461 caagaaatga atcgggaaat caacacgatc ggttccaaat caaatgcaat cgaaattaaa
    17521 aatcaagtcg tccagtctaa gacgatctta gaaaaaatca gagaacaagt acaaaatatt
    17581 gaatgacgaa agaggtagag ataatgaaat attatgacgt aacgttccac gaattaagtg
    17641 gaaaaactgt ggttaaacgg gatatccctt ctgaaaaaaa cggctttgac gtatggaaag
    17701 atgcctgtgc agactataat gagaacgaac tgtttatttt gatcaacgac ggtgcttacg
    17761 tgacgatgaa tcggaaattt attgttcgaa ttgacacaga agaggtagaa gatccgacag
    17821 aaaaagcacg ttctcgaaaa gatgagatca tgggtgtagt gaatactttg tccaatatgg
    17881 gcttttagca aggatgctga acaaagaaat acaataaata aacagaattg gatttcttcg
    17941 cttagcttct ttgctctagg aatacattta aaggttgtga caaaattcgt gtcacaatct
    18001 ttttttattt tgaataagtg atgttcaaaa accattttct cggtattaag ccaaatttat
    18061 gcaaaaatgt gagaaactat tttcacaaaa tttttcttca tactcgaaac taatcggctt
    18121 cttcacgacc tcttatacac ggcgttcaaa agcttgcatc tgcgataata accccaacca
    18181 aacgaaaaac atgagaagct gtttatgttt ggctgttctt attacttgat gcaggacagc
    18241 ttttttacga cctcttggta cagggtagct tttttaccaa tttctagcta taattaaaga
    18301 taaccttgaa agttgcagaa aaaaagtgca aaataaacga taagtacaaa aagactagtt
    18361 gaaaggacgt cgtcatgtca gagcgtggat tattaatcgt gttgtctggt ccatccggtg
    18421 taggaaaagg cacagtacga aaagcaattt ttgagagtga ggataatgat tttcagtatt
    18481 cgatctcaat gactacccgt aaaatgcgtg aaggggaggt cgaaggcgtt gattatttct
    18541 tccgaagcaa agaagagttt gaatcaatga tcgaagctgg agagatgctg gaatacgcag
    18601 aatatgtcgg gaattattat ggaactcctt tgtcatatgt caacaagaca ttggatgaag
    18661 gaaaagatgt tttcttggag attgaagtcc aaggagcaaa acaagtcaaa gaaaaagtgc
    18721 ccgatggtgt attcatcttt ttgactccgc cagatttagc ggaattaaaa tcacggatcg
    18781 taggccgagg aacagatgca gatgaagtaa tcgaaaaacg aatgaaagtt gcaaaagaag
    18841 aaatcgaaat gatggctctt tacgactatg ctgttgtaaa tgatgaagtg ccgctagcag
    18901 ttgaacgaat caaaaacatt attgccagtg aacattttcg agtagatcgc gtaatcggta
    18961 aatatattaa aatgttaaag gagatgtagt cttatgatgt taaaaccttc aattgactca
    19021 ctattagatc gtgtaaattc aaaatattct ctagtgattt tagcaagtaa acgcgcgcat
    19081 gaattggatg caagtgcaca gcctactcta gattcttttg attcagtaaa aagcgtcggt
    19141 caagctttag aagaaatcga tgcaggaaat gtagtcaatg atccacatcc tgaattaaaa
    19201 cgtgaacgtt tgaagatgga agaagaggaa cgtcaagcac aaaaagagcg tgaacaacac
    19261 gaattggaat cacgtatccg tgaagagcaa aaaatgtaat aaagaaaaat agagcgaaca
    19321 tctaaaaaga aaagcaaacc cagctgtttc cttttcaaac attcgccact tattgtctgg
    19381 tagaaaaggc tgtgtctgaa ctttttgaca cagttctttt ttttcttatc tgctgttaat
    19441 tttttgttag gatttggcag aagataagac aaatccatcg atctctagta aaataggcaa
    19501 ggaacttttt gtcgcttgat aagaaaagac aaaaaaagga ggaaacgaac catgacatta
    19561 acagctgaag tcattgtgga cgtacctact atgcagaccg atcagccatt cacttatttg
    19621 gttccctctg aaatggaaac agctttgcaa gtcgggatgc gagtagaagt cccttttggt
    19681 aatggcaatc gtcatgtcca aggatttgtt atggctatcc aaaaatcaga agaatcagct
    19741 aatccatctt tgaaggcagt tattcgtttg ctagaccttg ctccggtggt caatgaagaa
    19801 ttactttcat tagctgatta tatgaaaaaa attacttacg cattcaaaat cacttgtcta
    19861 caaacgatgc ttccgagtgt gatgaaagca gaatatgata aattgatcta tcctttggca
    19921 gacacaccag aagttcaggt attgtttgat cagcgagaag tgatttcctg gaaggaagca
    19981 gaagaagaag gcagcctttc tcaattgatt cgctggcgcc aagagcaatt agtagatatc
    20041 aaatatgaag tccatacacg aaataaagta aaaacgattc gtttggtccg ttctttgtta
    20101 actgaaaagc aaatcgaaga agaatgggca aaattacggc aaaatgccaa gaaacaaaaa
    20161 gaacttcttt tgtgtttatc tgaactgagc caagaagaac caattgctta tttcaaaaat
    20221 aaaggaatca gtactgctgt cttgaatcaa ggaaaagaaa agggctggct ggagtttata
    20281 gaatcagaac gttatcgcga cccttataaa gaccgagtat ttgaccaaac gacggcattg
    20341 gaactgaatg cagaacaaaa aaatgctgtc gaacaaatca tcacagcagg tcagcagcaa
    20401 aaagatcagg tctttttgct ggagggaatc actggcagcg ggaaaacaga agtatatctt
    20461 caggcaatcg ctgatgtttt gtcagaaaat aaaacggcta ttatgttagt gcctgaaatc
    20521 gcattgactc cgcaaatggt cgagcgattc aaaggacgct tcggagaatc agttgcggtc
    20581 ttacatagcg gactttccca aggtgaaaaa tatgatgaat ggcgcaagat tgagcgggaa
    20641 gaagcgcaag tagtagtagg cgcacgatct gctatttttg ccccgctgaa aaatatcggg
    20701 ctgatcatta tcgatgaaga acatgaatca agttacaaac aagatgagac accgcgttat
    20761 catgcgagag atctagcaat ttggcgcagt aaatatcatc attgcccaat agttctagga
    20821 agcgcgacgc cctctttaga atcaagagca cgagctcaaa aaggcgttta tcagttattg
    20881 cagttaaacc accgagccaa agcagctgca cagcttccag ctattgagat cgtagatatg
    20941 cgtgaagagt ttcagaatca tcggacctct actttttctg ctaatttgca ggaaaaaata
    21001 caaaatcgat tagacaaaaa agaacaaact gtcctactgc tcaatcgtcg aggatattcg
    21061 tcttttgtga tgtgtaggga ctgtggattt gttcttccat gtcctaattg cgatatctct
    21121 cttacactgc atatggatac acgttcaatg cgctgccatt attgcggaca tgaagaacct
    21181 attcccaatc gctgtcctaa ttgtggcggc aataaaatcc gctactatgg aacaggtact
    21241 caaaaagtag aagaggaact ccgagaactt tatccgcaag ctaggatttt gcgtatggat
    21301 gtagatacca ctcgaagaaa aggggcacac gaacagattt tacaaaaatt cggtgcaaaa
    21361 gaagcagata tattattagg gacacaaatg attgctaaag gtttggactt tccagaagtg
    21421 acattagttg gggtgttgaa tgctgatacc tccctgaatt taccggattt tcgttctagt
    21481 gagcatacat tccaattgct tacccaagtt tcaggacgag cagggcgagc ggaaaaagca
    21541 ggcgaagtcg tgatccaaac attcaatcct caacattacg ccatagaatt ggctaaaaaa
    21601 caaaattatg agcagtttta tcagcaggaa atgcatgtac ggcatcgtgg aggctatcct
    21661 ccttattact ttactgtgaa aataaccgcc agccatcctg aagaacaagt agcggcaaag
    21721 aaaatctttc agattgccaa tcagctaaaa gaagtgttaa gccctcaaag cttgctatta
    21781 gggccaacgc ccagcatgat tttgagagtg aaaaaccgat attattatca actaatcatc
    21841 aaatataaac atgaaccgaa tcttcctcat gttttggatg aaattttgaa tggaagccag
    21901 aaagaacagc gacaaggtct ttttgtggca attgacaacg aaccattaaa ctttatttaa
    21961 gaaagggcca aattatgcgc tatcctatat tgatccaccc taatgataaa ttaaaaagaa
    22021 ctgctcagcc aatcgatgta atcacggacg agacaatcgc tttattagac aatttatatg
    22081 agacaatgat tgcgaatgac ggaataggga tcgctgctcc tcaagtagga caaaacaaga
    22141 gaattgctgt gatcgaagta gatgaaggcg agaaatttga actgattaac ccagaaatca
    22201 tcgaagcaaa aggtgaaagc cttgacgtag aaggttgtct cagtattccg catgtctatg
    22261 gaacggtcaa gcgagcagat gaagtgaccg tgcgttatta tgatcgagac ggagaagaga
    22321 tagaagtgac ggcttttggc tatttagccc gtgccttcca gcatgaaatc gatcacttag
    22381 acggtatctt gttcattgag aaaatgatac agcagatccc ggaagaagaa cttgaagaat
    22441 atatggagga acatcttgat gactaagcta gtatttatgg gcacacctgc tttttctgtg
    22501 ccgattcttg aagggctgat agaatcaggt tacgagatcc aggcagtggt aacacagccc
    22561 gaccgtccag ttgggcggaa acgagtaatt acgccgacac cggtaaaaaa agcggctctg
    22621 aagcacggta tccgtgtgct gcagccagag aaaatctccg gttcgccaga aatggaagaa
    22681 atcattgaat tggtgcctga tgtgatcgtc actgcggcat ttggccaatt cttgcctgaa
    22741 aagttattgc aagtaccaaa attaggggca atcaatgtcc atgcttcttt acttcctaaa
    22801 tatcggggag gtgcgcctgt tcattatgcc atcatgaatg gggagaaaga aactggtgta
    22861 acgatcatgg aaatgatcaa aaaaatggat gcaggcggaa tctatgcgca agaaagcata
    22921 ccaatcacta aacaagatga tgtagggacg atgtttgaaa agcttagcct tttaggaaga
    22981 aagctattgc tagaaacatt gcccaatatt ttggatggac aaaaaccagt acctcaagac
    23041 gagtcagaag caactttttc gccaaacatc acacgggaac aagaagcaat cgattggcat
    23101 aaaactgcag aagaaattga caaccaagta cgcggaatgc gtccttggcc aattgcattc
    23161 acgacctatg agggcatacg ctggaaatta ttatcggttg aagtattggc agaaactaca
    23221 gacaaagcac ctggtacgat catccggaaa gacaaaaaaa atctatggat tgcttgcggt
    23281 aaacagaccg tcttagccat caatgaatta cagccagctg gtaaaggaaa acaggccgta
    23341 catgatttcc ttaacggaag cggtcagcaa gttcagattg gtcagcaggt gaaataatga
    23401 aaaaaatccc taaaaaaatg aaaaaatcag tacgatatat ggcattactg acattagagc
    23461 gagtgaacaa gggtggcgcc tactcgaatc ttttgttgaa tgagatgatc aaacaaagcg
    23521 agctaaatca aaaagatgtg ggtttattta cagaattgat ctatggtacg attagccatc
    23581 agcgactatt ggaattttac gttgcaccat tgatcgctaa agcaaaaaaa gtggatgact
    23641 gggtcaagat gcttctttat ctttctgtat accaattaga atttttagat aaagtccctg
    23701 cacatgctat tttaaacgag gcggtcgaaa tcgcaaaagt aaaaggaaat cctgggacag
    23761 ggaaatttgt aaatggtgtc ttgaggaatt atcagcgtca aggagcacca gatctgcaag
    23821 gcattgctga tcctatcgaa agactttctg tcaaaatcag cctgcctcta tggatgacga
    23881 aacgactggt ggatcaaatc ggatacgaag aaactgaaaa gctcggttta tcgctatatc
    23941 agcctagcca tgctagtgcc cgagtagata cacgccgact ttccagagat caggccatca
    24001 ctgttttgca agaagaagag atcacagctg aaaaaagcca agtcagtcca tacggtatag
    24061 tagcgaaaaa agggcattta gcttctagct cattgttcca cgatggcgtc atgacgatcc
    24121 aagatgaaag ttcgatgctt gtagcgccgt ctatgcaaat cgagcctgaa catcatgtct
    24181 tagatgcctg tgcagcaccc ggaggtaaga cgacgcatat tgcttctttt ttagatccgt
    24241 catgcggtgg agaagtggtc gctttagatg tacacgaaca caaagtcaaa ctcattgaag
    24301 ataatgcagc acgtctaggt gtctcttctg ctgtctacgc tcaaaagatg gatgctagag
    24361 aagtcagtaa acagttcgaa gcagagcagt ttgatcggat tttagtagac gcgccttgtt
    24421 caggtcttgg gctgcttcgg cgaaaaccag atattcgtta taaaaaacag ccggatgaat
    24481 tagcgaattt accaaaaatc caattggaaa ttttagaaag ctgcgcacca actttaaagt
    24541 cttccggtat tctgacttat agcacttgta cgatcttaaa agaagaaaac caagacgttg
    24601 tttctgcttt cttggaaaga caccctgatt ttgagaggat tgatgttttg accaatccta
    24661 tgctacaatc atcaatcgaa gagaaaatgt tgactttgta tccgcatcag ttttacacag
    24721 atggattttt catttgttgt ttgcgcaaaa agtaatgagg tgaaaaaatg cagattgaat
    24781 atcaatcaga tgtaggaaga cgacgtaaca caaatcaaga ctatgccagt gtatttacca
    24841 gccgttcagg tctcaagctt gctgtattag cagacgggat gggtggtcat cgtgccggag
    24901 acattgccag tcagatggcg gtaacgaatc taggcgaagc ttgggaaaaa caagaagctg
    24961 ttgacgatga aaaaatcgcc caatggttca ttcaatcgat tcaagaagaa aatatgctga
    25021 tttatcagcg tggacaagaa cagccggaat ataacggtat gggaacaaca atcgtagcgg
    25081 ccgctctgtc agatgactgg ttcacgattg ccaatgttgg ggatagcaga gcttatttga
    25141 ttcgtgaaga taaaatcatg caattgacgg aggatcattc tttagtcaat gaactagtca
    25201 aatcaggaga aatcagcgaa gaaatggcag tgaaccatcc aaggaaaaat attttaacac
    25261 gttctgtcgg catgcctgga acagtcgaag tagatgtttc tactcatctt tggcaactaa
    25321 aagatcgact gctgttatgt tcagatggct tgacgaacat gctgtctgaa gaagaaatag
    25381 gaacgatcat caatcaagaa ggcccattgt ctcataaagt aactgagctg attgatcgtg
    25441 ccaatgaagc aggcggagcg gataatatca ccgttcttct tatcgaatat aaggaggaca
    25501 gtgtatgatc gagttgggca aaaaattaaa tggccgttat caaatcactg gaaatatcgg
    25561 tagcggtggt atggcaaatg tatttttggc gcacgattta atcttggatc gtgatgtagc
    25621 agttaaagtc ttacgatttg actttcaaaa tgaccagact gcgattcgac gatttcagag
    25681 agaagcttta gcagcaacag aactcgttca tccaaacatt gttagtgttt acgatgttgg
    25741 tgaagaagac aacatgcagt atctggtgat ggaatacgtc aaaggaatgg acttgaaacg
    25801 atatatccaa acacattatc ctgttcctta tgaaacagca gtaaatatta tgcagcagat
    25861 cctttctgct atttctttag ctcataacca tcaaatcatc caccgagatt tgaaacccca
    25921 aaacgtactg atcgataatg aaggtgttgt aaaaatcacg gatttcggga ttgctatcgc
    25981 tttatctgaa acatcgatca cgcagacgaa tacgatgcta ggatctgttc attatctgtc
    26041 ccctgagcag gccagaggaa gtatggcaac gaagcagtca gatatttatg ctttagggat
    26101 catcctttat gagatgttga cgggtagcgt accgtttgat ggtgaatctg ctgtaacaat
    26161 tgctttaaaa cattttcaag atgatcttcc atctataaaa gcacttgatc caaatgtgcc
    26221 acaagcactg gaaaacgtga ttttacgggc aacggctaaa gaacctgcag accgttataa
    26281 gtcagccgag gaaatgtctg atgatctttc tacagtatta tcaccagctc gtgcaaacga
    26341 agaaaaatgg cagcctcatg tgatggataa tgaaacaaaa gttatcacac cccttacgga
    26401 agatgcacca atgcctgatt cattcaaatc tatgcctctg ccaaaagaca agccagaaaa
    26461 cgaaacctta ccagaggagg aagaagttct gcctgatcct aaaaagaaag gacagaaaaa
    26521 atggtggctg atcctattag ctattcttgc tgttttagga atcggaacag ctgtattctt
    26581 tgcttctggc gggcgaggag aagttgttgt tcctgatgtt tctgatcttt ctgaagcttc
    26641 tgctagagaa acactcgcaa aagcgaaatt agaagtagca acaaaaacag aagaaattgc
    26701 agatgacaaa atcgaagaag gtaatgtagt gaagacagac ccggcagcgg gaacgacagt
    26761 caagcaaaaa cgcgaagtca ctctgtatat tagtagcggc acgaaaaaaa tcaaattaga
    26821 tgattacagt ggtaaaagtt atgaagatgc cgaagcaagt ttgaaagaat tagggttttc
    26881 tgctgacttg attcaaaaag aagaggaatt cagttcagag gtagaaagcg gaatgatcat
    26941 ttcgcaatct ccatcagaag atacagaagt cgatcctaaa acagacacga ttactttcac
    27001 agtcagcaaa ggaccagaaa cagtgacttt gagtagtcat gtaggagaaa actacgaaac
    27061 tgttcgtaat tggcttgtta cacaaggatt caatatcaat aatattagtg ctacttatga
    27121 ctatagcgac actgtaccat caggttcgat catcagtcag gatccagcct cgggtgaagt
    27181 tgtagcagaa gaaacttaca tcaattttgt cgtaagccaa ggaacagagc ctaaattgaa
    27241 tgatatctcc ggctatacaa aaagcgaagc acaaagttat ttagctagtg tcggtgcaga
    27301 atatatcggg catgagactt ccgaatattc aaacacggtg gataaagata aagtgattcg
    27361 tacagtacca ggggcaggaa cgacaattac caaaggaatg gttgtgaacg taatttactc
    27421 taaaggacca gatccagcgt cttctagttc tgaaaaagac tcttcagaca gttctagcag
    27481 ctcaagtagc tccagtagca caagcaactc ttcaaaagaa gaatcttcca gctccacaag
    27541 ttcatcagct agcacctcag aaagttcaaa ataagtgctg aataaaatat atcagaaggg
    27601 aatgcgacat tgatttgttg cattctcttt tttgtgtttc tatctctttt gatcaatttt
    27661 caagccatat actttgagaa gtttgcatgg aataacacct actttcctct tcgtatggta
    27721 aaataaacaa aaacggaggt ggcacttatc aaaggacaaa tcagaaaagc aatcagcggt
    27781 ttttactatg tatacgcaaa cggagagacc tatcaaacaa gaggaagagg aaatttccgg
    27841 aatcggaaaa tcacgccgct tgtcggagat caggtgatct ttgagagtga aaatcaaaca
    27901 gacggctatc ttctggaaat cttgccgaga aaaaatcaat tagttcgccc accagtcaca
    27961 aatgttgatc ttggaatcat tgtcacaagt atcgtagagc ctgcattttc ttataatcta
    28021 cttgaccgct ttctagttac cttggaatac gagtcgatcg aaccgattat ttttttgaca
    28081 aagaccgact tgtcagacga cccagaaaaa atcgaagaga tcaagcaaac ctatactaaa
    28141 ataggctatc cggtgattgt acccaatcat acgggtgata cgacagaact gatccgttac
    28201 ttcccagaac ggctatcggt ctttatgggt cagtctggag caggaaaatc gacgctactg
    28261 aatcaaatat caccggaact gaatttagca acaggagaga tttctgatgc acttggaaga
    28321 ggacggcata caacacgtca tgtggaactg attcctatct atggcgggtt agtagcagat
    28381 acaccaggat ttagctcgat tgactttttg acaattgaag caggagaact gccgagacaa
    28441 tttcctgaat ttgtagaagc ttctgtccat tgcaaattta gagagtgcat gcatgctaaa
    28501 gaacctggct gcgaagtaaa acatagagta gaaacaggtg gtatcgcaca gacacgttac
    28561 gacaattatt tgcaattctt acaagaaatc gaaaaaagaa aaccaatgta tgcaaaaaaa
    28621 gaaggtaaac gaaaatagga ggaaacaaga atgaagatag caccatccat tttgagtgca
    28681 gattttgcca atttacaaag agacattgag ttagtggaaa aaggaggcgc agattacatc
    28741 cacgtagatg taatggatgg ccgatttgtc ccaaatatca cattagggcc aaatattgtt
    28801 caggcaatcc gtcctgttac taaattaccg ttagacgtcc atctgatgat cgttgatcca
    28861 gaaaactaca ttccagcatt tgcaaaagct ggagcagata tcattactgt ccacgtcgaa
    28921 gcaactcctc atattcatcg ggcacttcaa atgacaaaaa gcttaggagt taaagctgga
    28981 gtagttatca atccaggaac accagtctcc atgatcaaac atgttttgcc tttggctgat
    29041 caagtactag tcatgactgt caatcctggg ttcggcggac aaagctttat tgaagaaaca
    29101 gtagaaaaaa ttacggaact cgctgatcta cgtagagaga acaattggac ttatgcaatc
    29161 gaagtagatg gcggaatcgt tccagaaaca gcaaaaatct gtaaagatgc aggggcggat
    29221 gtcttcgttg caggatcata tatctatgga gccgaaaatc cagtcggaca aatcgaacga
    29281 ttaaaagagg cattagacta aatgaaaatt ttattagctg ctggaggtaa tccgcaaaac
    29341 tggccgcagt ctctgctgtc caaatacgat gcctatgtcg ggatagaccg tggtgcgctg
    29401 tacctgcttc aagcaggcta cccagtagat ctggcagtgg gtgattttga ttcattgtcg
    29461 aacgaagagc agaaggaagt atttgcaaaa gccaaagagt atacccgttc ccctgcagaa
    29521 aaagacgata cagatacaca gttaggtatc ttaaaaacat ttgaacgtta cccgcaagcg
    29581 gaagtcactt tgattggagc tacgggcgga agattggatc atcttttagc caatctctgg
    29641 ttaggtatgg aacctcgctt caaacccttt gcccatcagc ttattatcag agattgtcaa
    29701 aattatttgt cttattattt gcctggagag catatagtaa gacagatacc agatatgaag
    29761 tatttagctt attgctgctt gactcccgtc tcgaatctta cattaaaagg cagcaagtat
    29821 gtattggaaa ataaagaagt agaatatccg acatcttatg caagtaacga atttctttca
    29881 gataccgcag aattttcttt ttcagatggt atgattgcag ttattcaaag caaagattga
    29941 tccataaaca aataaaaaaa tttgtctttc aggctaaaaa tgacagaatc tattatttgc
    30001 taaaaaaaag cttaagagga gcaacttacc ttttgtttca cggcatttag tgataaaatt
    30061 gaacacaaga gtctaattaa ttcgaggtgt ctttgtgaag aagttcaatc ctaataaaaa
    30121 tattattatt accttggtta ttgttatcat cgtggtgacg atactaagtg tgaccgctgc
    30181 gcagcgagcg aatgaaggaa agcccaattt cttccaatcc atcgttaatg acagtgttgc
    30241 aatggttgat aaaacaatct ctgccccagt acggtgggta cgaaatggag tagattctgt
    30301 ccataatctt ttttcaactt atgccgaaaa tgagcggcta aaagaaaaaa tcgatagcta
    30361 tgatgacgtt tcacagcaaa acaagaatta cgagagagaa atcgaagcgt tgaaaaaaga
    30421 acttgagttg aatcagacgc tgactagtta cgaaaaagta acggcaaatg tcattactcg
    30481 ttcgcctgac agttggcagg acatgctgat cgtagacaag ggttctaaag acggtatcga
    30541 agcaaatatg gcagtcatgt cgcaaaaagg attgattggc cgagtgatcg aggtcaatac
    30601 ggcttcgtct aaaatcgaat tactgtcatc ctctaatgaa agctccaatc attttccagt
    30661 acgggtatct tcggctaatg gcgaagcgtt tggtttgctt aaaaactatg atgaaaagct
    30721 ccatgcctta gtggtgaccc aattaactgg tgatacggat atcaaagaag gggatgttgt
    30781 ccagacatcc ggtcttggag ggaattctcc agctaacttg ccgatcggta cggttattaa
    30841 aacgaaacca gatagttatg ggctggatcg ggaagtttat gtgaaacctt atgcagaaat
    30901 gtatgacgtg tcagttgtga cgattgtcca acggctagcg gaggattagt ccatgttgaa
    30961 gaaagaaaca atgaagtact atctgccaat cgttttgttc tttttgatgt tgatagatgg
    31021 tcatttaaca agaatgctag gggagtggtc gaaaggcacc tatatgtcaa atgcccactt
    31081 tctgatattg gcattattat gttgcagtat ggcgtttgaa aaacgttatt tactgattac
    31141 cacgattgtt ctcggggcta tctatgacgc ttactatatt ggcgttatcg gtatctatgc
    31201 agtagctctc cctttaattg tatggttgat gtatgtaatg aaagacgtta tccatgtcaa
    31261 catctttact gaatttttca gtatgatcat ctttgtcacg ggttatgaat tgtttacgat
    31321 ggtggtccag ttgattttta aattagcagt agtaaataac acgtatttta ttacaaggtt
    31381 tttaggacct acactgctgt tgaacatgat tatatttgta ttattcattt ttccctttaa
    31441 gaaattattc agtgatgaat aatttctttt tttattttta ggtcgaaagc gaaaaagaat
    31501 cgttgttatc ttaagaaatg ctagacttaa cgcggctttt tcatgatcat tttctgaaac
    31561 gaacagtttt gtcattttta aaagttgttt tgaaacattt ttgtaa
//
LOCUS       DACDMM010000016        30902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDMM010000016 DACDMM010000000
VERSION     DACDMM010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.016"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            3..731
                     /locus_tag="IWD16_000562"
     CDS             3..731
                     /locus_tag="IWD16_000562"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP9087646.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            731..985
                     /gene="purS"
                     /locus_tag="IWD16_000563"
     CDS             731..985
                     /gene="purS"
                     /locus_tag="IWD16_000563"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP9087647.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            987..1658
                     /gene="purQ"
                     /locus_tag="IWD16_000564"
     CDS             987..1658
                     /gene="purQ"
                     /locus_tag="IWD16_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP9087648.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1659..3881
                     /gene="purL"
                     /locus_tag="IWD16_000565"
     CDS             1659..3881
                     /gene="purL"
                     /locus_tag="IWD16_000565"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP9087649.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3866..5305
                     /locus_tag="IWD16_000566"
     CDS             3866..5305
                     /locus_tag="IWD16_000566"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP9087650.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5337..6380
                     /locus_tag="IWD16_000567"
     CDS             5337..6380
                     /locus_tag="IWD16_000567"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP9087651.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            6377..6955
                     /gene="purN"
                     /locus_tag="IWD16_000568"
     CDS             6377..6955
                     /gene="purN"
                     /locus_tag="IWD16_000568"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP9087652.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            6952..8487
                     /gene="purH"
                     /locus_tag="IWD16_000569"
     CDS             6952..8487
                     /gene="purH"
                     /locus_tag="IWD16_000569"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP9087653.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8503..9777
                     /gene="purD"
                     /locus_tag="IWD16_000570"
     CDS             8503..9777
                     /gene="purD"
                     /locus_tag="IWD16_000570"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP9087654.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9899..10813
                     /locus_tag="IWD16_000571"
     CDS             9899..10813
                     /locus_tag="IWD16_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP9087655.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(10946..12511)
                     /locus_tag="IWD16_000572"
     CDS             complement(10946..12511)
                     /locus_tag="IWD16_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9087656.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12504..13955)
                     /locus_tag="IWD16_000573"
     CDS             complement(12504..13955)
                     /locus_tag="IWD16_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9087657.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(13969..14985)
                     /locus_tag="IWD16_000574"
     CDS             complement(13969..14985)
                     /locus_tag="IWD16_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9087658.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15205..16293
                     /locus_tag="IWD16_000575"
     CDS             15205..16293
                     /locus_tag="IWD16_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9087659.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16347..17873
                     /locus_tag="IWD16_000576"
     CDS             16347..17873
                     /locus_tag="IWD16_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP9087660.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17875..19080
                     /locus_tag="IWD16_000577"
     CDS             17875..19080
                     /locus_tag="IWD16_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9087661.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19337..20314
                     /gene="guaC"
                     /locus_tag="IWD16_000578"
     CDS             19337..20314
                     /gene="guaC"
                     /locus_tag="IWD16_000578"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP9087662.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20578..22002
                     /locus_tag="IWD16_000579"
     CDS             20578..22002
                     /locus_tag="IWD16_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9087663.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            22463..23572
                     /gene="pdhA"
                     /locus_tag="IWD16_000580"
     CDS             22463..23572
                     /gene="pdhA"
                     /locus_tag="IWD16_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP9087664.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23575..24552
                     /locus_tag="IWD16_000581"
     CDS             23575..24552
                     /locus_tag="IWD16_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP9087665.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            24577..26220
                     /locus_tag="IWD16_000582"
     CDS             24577..26220
                     /locus_tag="IWD16_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP9087666.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26228..27634
                     /gene="lpdA"
                     /locus_tag="IWD16_000583"
     CDS             26228..27634
                     /gene="lpdA"
                     /locus_tag="IWD16_000583"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP9087667.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            27796..28071
                     /locus_tag="IWD16_000584"
     CDS             27796..28071
                     /locus_tag="IWD16_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP9087668.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28077..28844
                     /locus_tag="IWD16_000585"
     CDS             28077..28844
                     /locus_tag="IWD16_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP9087669.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29012..30841
                     /gene="typA"
                     /locus_tag="IWD16_000586"
     CDS             29012..30841
                     /gene="typA"
                     /locus_tag="IWD16_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP9087670.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
BASE COUNT        10109 a         5316 c         6990 g         8487 t
ORIGIN      
        1 acatggagaa gaaagaactt ttgtatgaag gaaaagccaa aaaaatatat cttacttctg
       61 atgagaactt gatttggata gaatatttag atcaagcaac tgcgttgaat ggcgcgagga
      121 aagaccagat aagtggaaaa ggagaattga ataatcaaat cacaagtgca attttccgtt
      181 atctgactca aagaggaata aaaaatcatt ttattgaaca attgtctaaa accgaacaat
      241 tagcaaaaaa agtacaaatt atccctctgg aagtggttat ccgaaatatt gcagcaggaa
      301 gtttctcaaa acgtctaggt gttcccgaag gtaaagaatt catcaaacca atcattgaat
      361 tctactataa ggatgaccag ttggatgatc catttatcaa tgaagagcat atctatttct
      421 tacaacttgc aaaaccggaa gaaatcagtg agctgaaagc ccaagcgttg gctattaatg
      481 aaattttgac cgaattattt gctaaggtcg gtttggtttt agtagatttc aagctagaat
      541 ttggccggtc gaatggacaa attttgcttg cggatgaagt aacaccggat acttgccgat
      601 tatgggatga acgtacgaat gaacatatgg acaaagacgt ttaccgcaga gatctaggtg
      661 atttgatccc agtctatcaa gaagtatata accgtctatc ggaactaact gaagaaaagg
      721 agcgagtatg atgtatttcg taaaagtata tgtaacctat aaagattcag tgttagatcc
      781 tcaaggggag gcagtaaaaa aagcagtcca tcgtatggga tatgaaacaa tcgaagatat
      841 ccgaatagga aaatactttg agatacaact tgctgatgca gaagaaccaa tcgatacagt
      901 gattgaaacg atttgtgaca agctgcttgc aaatgtaaat atggaaacat accgatatga
      961 aatcaaaaaa atggaggaag tataacatga aatttgcggt catcgttttt ccaggttcaa
     1021 attgtgacgc agatatgtta tgggcagttc gcgagatcat gggcgctgat gccgaatttg
     1081 tacggcatga tgagtcctct ttagaaggat ttgatggggt tttactgccg ggaggattct
     1141 cctatggtga ctatttgaga tgcggtgcga ttgctcggtt ttctacaatc atggaagaag
     1201 tgattcgttt tgctgaagaa gggaaaccag tattcggtac atgcaatgga ttccagatat
     1261 tgacagaagc aggacttttg ccaggtgtac tgagacgaaa tgagtctttg cattttattt
     1321 gcaaaactgt accattgaaa gtcaacccca agacggcctt tacttcagag tatgaagccg
     1381 acgaaataat ctatcttcca gttgctcatg gggaaggaaa ttatttctgt gacgaagaaa
     1441 cacttcaaga actaaaaaaa aataatcaga ttgcctttac ttataatgac aacatcaacg
     1501 gcagcgtgga aaacattgca gggattacga acaaagcagg gaatgtgtta ggaatgatgc
     1561 ctcatccaga acgtgcagta gaagacttgc taggatcaga agatgggaaa cggttctttg
     1621 cttccatttt aaaaaacttc ggaaaggtga ccgtatagat gaaaacattg aaagaaccga
     1681 cagctgaaga gatcaagaaa aaaagaatct atgccgaatg gggattgact gatgaagaat
     1741 atcgaatgat cgaagaagat atcttaggaa gaatgcctaa ttatacggaa actggactgt
     1801 tttctgttat gtggagcgaa cattgttcct ataaaaattc taaaccggtt ctacgcaaat
     1861 ttcctactag tggacctcat gtgttgcaag gccctggaga aggcgcaggc atcgtcgaca
     1921 tcggtgatgg actagcagtt gtcttcaaag cagaaagtca caatcatcct tcagccgtgg
     1981 agccttatga aggagctgcg acaggagttg gcggaatcat cagagatatt ttcagcatgg
     2041 gggctcgtcc aattgcgttg ctagattcat tgaggttcgg cgaattagat aatccaagaa
     2101 cgaaatattt actagaagaa gtagttgcag gtatcggcgg atacggcaat tgtatcggca
     2161 taccgactgt aggcggcgaa attgcatttg atccttgtta tgaaggaaac ccattagtca
     2221 atgcaatgtg cgtaggtttg attgaacaca aacatatcca gaaagggcaa gccgctggcg
     2281 tcggtaattc gatcatgtat gtaggtgcaa aaacaggtcg cgacggtatc cacggagcaa
     2341 cttttgcctc agaagaattt aatcaagaag aagaacagca acgttctgct gtacaagtag
     2401 gcgatccttt tatggaaaag ctgctgcttg aagcgtgttt ggaattaatc ttgcatcatt
     2461 cggattctct aataggtata caggatatgg gagcagcagg acttgtttca tctagtgcgg
     2521 aaatggcttc aaaagctggc tcaggactta tattgacatt agatgaagtc ccacaaagag
     2581 aaactgggat gacaccttat gaaatgatgt tatcggaatc acaagaacgg atgttgattt
     2641 gtgttaaatc aggagaggaa gaaaaaatcc aagaactatt tcagaagtat gatctagatg
     2701 cagtcactat cggtaaagta acagacgacg ggcaatatcg gctatatcat caaggagaag
     2761 aagtagcaaa tctgccagtc gatgcattag ctgaagatgc accagtttat cataaagaaa
     2821 tgaaagaacc agctcgaatc gtagaatttc agcagttagc tccttatcag ccagttatcg
     2881 aagaacctgg acaagtattg cttgatctgc tgcagcaacc gacgattgct tcaaaacgtt
     2941 caatctacga aacttatgat tcacaagtac aaaccaatac agttgtccgt ccaggaagcg
     3001 atgctgctgt gatgagggtt agagggacga ataaagcgtt agctatgacc acagactgca
     3061 acgcgcgcta tctgtatttg gatccaaaaa tcggaggaca aatagcagtg gcagaagctg
     3121 ctagaaatat tattgctagc ggtggtaagc ccttggcgat caccgactgc ctgaactatg
     3181 gttctcctga taaaccagaa gttttttggg aattatcgac ttcagctgac ggaattgctg
     3241 ctgcgtgtga aacgttagga actcctgtta tttcaggaaa tgtctcttta tataatgaaa
     3301 cggatggtca agcaatctat ccaacaccaa tgattggtat ggtcggattg attgaggatc
     3361 ataaacatat cactactcag gaattcaaaa aatcagggga tctaatctat atattaggta
     3421 agacgtttgc agattttgat ggcagtgaat tacaaaagat gcagcttgga agaatcgaag
     3481 gtgtgatccg aaactttgat ctatccattg aaaagagaaa ccaagagtta gtattgacag
     3541 ccatccaaaa cggattgatt gaaagtgctc atgactgttc agaaggtggc ttggcaattg
     3601 cactggcaga atcagctttc aaacatcagc tagggatatc tgtacagttt gaattgtcct
     3661 ctgctcaatt atttgcagaa actcaatcaa gatttgtgtt gacagtcgca ccggaaaata
     3721 agacacgatt cgaagaaatg atgggagatg ctgctgtttt agcaggtaag gtgacggatg
     3781 aagcgataat cgaaatttca gcaactgacg gtcagatcaa aatagaaact gcagtagcaa
     3841 gaaagtgctg ggaggatgca atcgtatgtc ttatgaagtg aagagtttaa atgaagaatg
     3901 cgggatcttc ggtgtttggg gtcatccaga cgccgctagt gtggcatact tcgggctcca
     3961 tagtcttcag catcgaggac aagaaggtgc aggtatcgtg tccaatgatc gaggaaagct
     4021 taaggggcat cgagatttgg ggctgctttc tgaggtattc aaagatcaaa gaaaattgca
     4081 gcagttaaca ggagaagctg caatcggaca tgtacgttat gcaacagcag gcaatggaag
     4141 tgtggacaat attcagcctt ttctattcaa atttcatgat cagtcgatcg gcttagcgca
     4201 taatggtaac ttgatcaatg ctcgttccct tagaaaagag ttagaggaaa atggggcgat
     4261 cttccattct aactccgata cggagatatt gatgcatttg atccgacgaa gtgaagaaac
     4321 agatttttta aataaattaa aaagtgcttt gaatgaagtc aaaggtggct ttgcctattt
     4381 attaatgaca gaaacggcaa tgattgccgc tttggaccca aatggttttc gtccattagc
     4441 aatcggacaa atgaaaaacg gagcatacgt gattgcctcg gaaacttgtg cattagaagt
     4501 tattggggca cgttttataa gagatgtcca gcctggtgag atagtgatca tcaatgacga
     4561 aggaatccag attgattgct ttacgaagga cacgcagctt tctatttgtt cgatggaata
     4621 tatctatttt gccagaccag attcaaacat cgcagggatc aatgtgcata ctgcacggaa
     4681 aaatatggga cggaatttag cgaaagaatc gccagtaaaa gcagatatgg tcgttggcgt
     4741 acctaattct tctttgtcag ccgcaagcgg ttatgcggaa gctagtggaa tcccttatga
     4801 aatcggatta gtaaaaaatc aatacattgc ccgaacattt atccagccaa cgcctgagct
     4861 aagagagcaa ggtgttcgaa tgaaattatc tgctgtacga ggagtagtgg aaggtaaaaa
     4921 ggtgattatg gtcgatgatt caattgttcg gggtacgaca agcagacgga tcgtccatct
     4981 tttgaaagaa gcaggggcaa aagaagtcca tgtgaggatt gcttctcctc ctttgaaata
     5041 tccatgtttt tacgggattg atattcaaac gcgtaaagaa ctcattgcgg caaatcacac
     5101 gattgatgaa atcaaagaat gtatcggtgc agattcttta ggttttttaa gtgaagaggg
     5161 tctgatcgaa tcgatcggtc tggaaaaaga tgctccgtat tcaggattgt gtatggctta
     5221 tttcaacgga gattatccga cgccgttata tgactatgaa gaaaaatatc aggaatcact
     5281 aaaagaaaaa gtatcgttct tttaaaagca gaccaagaag ggagtatatg aattcaatgg
     5341 caaatgcata cgcaaaagct ggcgtagatg tcgaagctgg atatgaagta gtagaacgga
     5401 tcaagaagca tgtcaaaaaa acggaacgtt taggtatgat gggcgctata ggaggattcg
     5461 gcggttgttt tgatttatcg gtctttcaat taaaagagcc agtccttgtc tcggggaccg
     5521 atggcgtggg gacgaaatta gcagtggcaa tcgaagcaga caaacacgat acaatcggga
     5581 tcgactgcgt agcgatgtgt gtcaatgata tcttagcaca aggcgcagag ccgttatatt
     5641 tcttagacta cttagcaacg gggaaaaatg atccgttacg tttggaaaat gtagtaaaag
     5701 gtgtagcaga tggatgtgtt caaggaaata tggcgctgat tggcggagaa acagctgaaa
     5761 tgcctggtat gtactcaggt aatgattatg atctagccgg atttgctgta gggatagctg
     5821 aaaaatctca attattgaca ggagaaacga tcaacccagg agatgtgttg cttggaatag
     5881 catctagcgg gattcattca aatgggtact ctcttgttcg caaaattttc tttgagcagc
     5941 agcagttttc tttatcatca aaactgccag aattgaaaaa aacacttggc gaagaattgc
     6001 ttgagccaac gaaaatctat gtttcttcct tgcttccgct gatcaaagaa aagaaaataa
     6061 aaggagctgc acatatcact ggtggagggt ttgtagaaaa tatcccgcga atgttaccag
     6121 aatctatggc tgcacaaatc aaacttggct catggccgat tcttccaata ttcggctgtt
     6181 tacaaaaata tggtgaaatt cctgagatgg agatgtatga aatcttcaat atgggcatcg
     6241 gtatggtcct ggcagtcgaa gaatcgaaag taaaagaaat agtacacgaa attgagcaag
     6301 ctggcgaaaa agtttatgaa atcgggaaag tgatcgccaa agaaaaacat cgtgtggtgt
     6361 ttataggaga agaacgatga gaatcgctgt ttttgcttct ggaaacggaa gcaactttca
     6421 agcgctagcc gattatctat cgaaaaaagg attggaatct tcgattgact ggttgttttg
     6481 tgatcagcct gaagcatatg ttttgaaacg tgcaactgcg ttgagtgtac cggcagattg
     6541 tttttcacct aaagaatttg attcaaaaaa agagtatgaa gaagcaattt tgcataaatt
     6601 gaaagaaaaa aagatcgatt tgatcgtatt agctggatat atgcgaatca ttggaccggt
     6661 attgctgaaa aattatgata aaagaatcat caatatccat ccgtctcttt tacctgcctt
     6721 ccctggtttg catggcatac gtgatgcctt tgaagctgga gtaaaagaaa cgggtgtcac
     6781 aattcattat atcgaccaag gtgtagatac tggaccaatc atccggcagg aaaaagtgag
     6841 aattgaacag gaggacacgt tcgattcttt agaagaaaaa atccatagag tagagcatcg
     6901 aatctatcca gaagtgatta gtgaaattat tgaaaatgga ggctatctga aatgacaaga
     6961 gcattgatca gtgtatcaga taaaactggg attgtagcat tcgcccaagg tttgattgaa
     7021 caaggagtag aaatcatttc aacaggagga acaaagcatg cgttggataa agctgggatc
     7081 aaaacgcttt cgatcgaaga agtgacaggg tttcctgaaa tgatggatgg ccgtgtcaaa
     7141 acacttcatc ctaaaatcca tggtggtctt ttaggaagac gcgatctccc tagccatatg
     7201 gaggcaatgg aaacggaaaa tatccaacca atcgactttg tttgtgtcaa tttatatcct
     7261 ttcaaagaaa cgattatgaa accagatgtc gaaatagcag aagctattga aaacattgat
     7321 atcggtggtc ctagtatgct ccgtagtgca gcaaaaaacc atgaatttgt cacctctgtt
     7381 gttgatccat ctgactatga agaagtactt tcagaattac gtcagtatgg gcatacttca
     7441 ctttctttca gacaaaaact agcggcaaaa gtctttcgtc atactgcggc atatgatgca
     7501 ctgatcgctc aatacttaac ggattgggtg gaagaaaaag aaccggaaaa attgacactt
     7561 acgtatgaac gaaaacaaac gcttcgttac ggtgaaaata gccatcaagc cgctgccttt
     7621 tatcagtccg ctcttctgga aaaatattcg atcgcagcag caaaacaact ccatgggaaa
     7681 gaactttctt ataataatat ccgagatgca gatgctgcgc tacggatcat gaaggaatat
     7741 gacgagccaa cggtagtcgc cttgaaacac atgaatcctt gtggtattgg gacagcatct
     7801 acgattttag aagcgtggga agcagcgtat gaagcagatc ctgtttcaat ctttggtggg
     7861 atcatcgttt tgaatcgaga agtagatctg ccaacagcag aacagatgca taagctgttt
     7921 ctagaaatca tcattgcccc ttcttataca caagaagctt tggaattact gcaaaaaaag
     7981 aaaaacatcc gattactgac agtagacttt gcgcaggaag aaaatcaaaa tcgagaagaa
     8041 acggtctcag tcttaggtgg acttcttgta caagatcaag atctggcaat agaaaatgaa
     8101 gaagagtgga aagtcgtgac aaaacgtaag ccgactccag cagaagcaaa agcattagcg
     8161 tttgcttgga gagctgtcaa acatgtgaaa tctaatgcga tcgtactggc aaacgaacgt
     8221 cagacagtag gaatcggagc cgggcagatg aatcgagtcg gttcagtaaa aattgcgatt
     8281 gaacaagcgc aagagaaaat ggaaggtgca gttttagcta gcgatgctta ttttccaatg
     8341 gatgattcgg ttgaatatgc agccaaacat ggtataaaag caatcattca gccaggagga
     8401 agcatcaaag accaagattc tattgatatg gcgaataaat acgatctagc aatggtcttt
     8461 actgatgttc ggcattttag acattagaga ggagtcggat aaatgaacat tttggtgatc
     8521 ggtgcaggtg gtcgtgaaca tgcaataagt aaaaaacttt tagccagtcc gaaggtggat
     8581 acggtgtatt gtgcgccagg aaatccgggg atgacaaaag atggtatcaa ttgtgtcgct
     8641 atcagtgagc gagatcattc agcgttaatc cattttgtta agaaagaggc tattgattgg
     8701 agtatcgttg gtcctgaagt tcctttgtta aatgggatcg tcgatgattt tatggcggaa
     8761 ggattgaaaa tcttcggtcc gacaaaagca gcagctctga tcgaaggttc gaaaagtttt
     8821 gctaaacaat taatggatac ttatcaaatc ccgacagcta aatccagaag ttttgaatgt
     8881 tacgaaaaag cagcagcgta tatcaaagaa aatggtgcgc caatcgtagt gaaagcggac
     8941 ggcttagcag ctgggaaagg tgtagtcgtg gctaccacag tgaaggaagc gctggatgcg
     9001 gcagaacaaa tgttgaacca gcatcgattt ggggatagta gcaaaaaaat cgtcgtagaa
     9061 gagtttttgg caggagaaga attctccctt ttggcgtttg tccgtaatac caaggtgtat
     9121 ccgatggtca tttcgcaaga tcataagcgg gcatttgata acgataaagg gccaaataca
     9181 ggcggaatgg gtgcttattc accagtaccg caaatttcaa aaaagatgat tgaaaaagct
     9241 gtggaagaag ttttaaaacc agcggctttg ggaatgaaag aactaggacg ctctttcacg
     9301 ggtgttttat atgcgggttt gattgctacg aaggaaggac caaaagtgat cgaattcaat
     9361 gcgcgtttcg gtgatcctga aacgcaagtc gttctttctc ggttgaagag tgactttgcg
     9421 caagtaatcg atgatttatt agagaatcgt acagtagagt taaagtggca gcaagaagga
     9481 tacaatgtag gcgttgtggt tgcagctgcc ggttaccccg aagaatatga gacaggaatg
     9541 gttctaccag attttctgga agaagagctt tctgtttact atgctggagt ttcctcaaat
     9601 aacggcaaac tagttggagc aggaggacgt ctttatttag tagaagcata cggtgagacg
     9661 atcaaagaag cacagaaaaa aatttacacc gcgttaatgg ataaaacgac agatggaaca
     9721 ttttatcgta cagatattgg agaaaaagct gccaatagcg aactgatcgt gaagtaaatc
     9781 agctgcttcc atttataaaa aatacaacaa acataatcgg aagcgccgat tttagtgaag
     9841 ccttttcata aagagtgtgg tacacttttg ttgtgtatat aaataaggag gagttcagat
     9901 gaaaaaaatt caattaggta cttctgatca gaaagcagct gcagtgatct taggctgtat
     9961 gcgtatcaac aatgcggaag atccagtaaa agtgattgag acagcttatg aaaacggcat
    10021 tgattttttt gaccatgcag atatttatgg aaacggtgaa tgtgaagaaa tctttgcaga
    10081 tgctctagca aaaacatcaa tcaaacgaga agatcttttt attcaaacaa aatgtggaat
    10141 cgttccaggt gtgatgtttg atttttctaa aaaacatatc attgaatctg tagaaaaaag
    10201 tttgacccgt ctgaaaatgg attatgtgga tgctttactt cttcatcgtc cagatacatt
    10261 ggtagaacca gaggaagtag ctgaagcgtt tgatgaattg gaacggtcag gaaaagttcg
    10321 ttattttggc gtcagcaatc aaaaaccaat gcaaatcgaa ttgctgaaaa aatatgttcg
    10381 tcaaccatta gtagctaatc agctgcaatt tggcttgaaa catactggaa tgatcgatca
    10441 agggattcat gtcaatatga cagatgaagc aagtatcgat catgacggta gtgtacttga
    10501 ttattcccga ttgcatgata tgacgatcca agcatggtca ccataccagt atggattttt
    10561 tgaaggtgta tttatcggga acgaaaaatt ccctgaatta aaccaaacat tagaagaact
    10621 agcagctaaa tatcaaacga caccaactgg cttggcttct gcttggattt tacgccatcc
    10681 agccaatatg caagtgatcg ccggtacgat gaacgtaggg cgaatcaagg aaatcgctca
    10741 agcttcagaa attaatatgt ctcgggaaga ttggtacgaa ttgtaccgtg ctgctggaaa
    10801 cattttacct taaagattgt cataagcagg tcaaaagggc tataaattaa aaagacagaa
    10861 taacttgaaa gctgttttca gcttcgagat attctgtctt tttttattgc aaatggaaag
    10921 agtatcattg tacggatttc gtttattagt tatttttgtt tttaaggaaa agctgtgtcc
    10981 gatattcttt tggagaaatc ccttgttttt tcttaaataa ataactgaaa tagttagggt
    11041 cattataacc aatttcaaaa gcaatatctg aaatacgata gtctgattgc tgcaataatt
    11101 tttttgctaa ttcgattctt tgttctgtca gataatcgat gaatgtttgg ttaaacgatt
    11161 gagaaaaaat cgtactgaaa tgagagggac ttaatgcaac ttgttgagcg acccaagtta
    11221 aggacatatc tggatcggta aaattttctt tgataaaatc caatgcatgg ttcaaaattg
    11281 gctgatattt gttccttgaa ggctgtgagg gcagagcagt taagaaagaa atgaacatat
    11341 aaactgtcga acggtaaagc gagtcgtcgg cgctgattgc taagcgttga ctggtggatg
    11401 tgcaatgaac catttcttgc gggattttct tttctttcag gcagacatgc tggagtagct
    11461 tagtcaagac aaataaacga tataatcgtg ttcgagttgg tgtgccttgt ggtttcgtca
    11521 agtgttcgat gtacttagat acttcgtttt tcgataatga agacaaatca agtgagaggt
    11581 caaagtaaaa gacagaatca actggaagtt tcgcttcttg aatcaaatcc tcatagctga
    11641 taatttgttc tttaagggaa ctggcggttt caagtatcat ctgcgtgatc tcaaaagatg
    11701 caggtaattc gctgattctt tctgcgacag gaccaatggc gacagaaaaa ttattttttt
    11761 gtcccttttc taattcatgg accaacgttt gggctacctg ataactggtc tcgatcacat
    11821 tttcttcaag agaatcaact attaaaaatt tgatgaatcg agaggaaaca gcagagaaga
    11881 tcacttgttg attctcccta aataaaaact tcaaataatc actgagatga gcgtaatctt
    11941 caaagtctgt actgacttgg tttgctgcta ataatacggt catttttttc cctgctaaag
    12001 agcgattgaa agattcagca agtttcaatg tttcactgat cgtaagtgag cctttgaaca
    12061 aaccgttcaa gaaatgattt tttttcaatt cgaaaacaag atcagaagaa gtgtgcgttt
    12121 ctttttgctg caaatctaaa gcagttactg ctttttctat tgcttgtttc aattcgctac
    12181 ttttaatcgg tttaagcaga taatcatctg cctgtacctg gattgctcct ttcatataat
    12241 cgaaatcgtc gaatccacta ataaatatga gacgagtcca aggaagtagt ttccttgctt
    12301 cttttgaaaa agtcaacccg tccatgaaag gcatttggat atcagtcaga atgatgtcgg
    12361 gctgtaagtc gataattgaa gacagtgcca tctcaccatc tccagcttcg ccgacataag
    12421 tgattggata ataagaacca aggacagtta tttgttcccg caagttattg cgaatgaggt
    12481 attcgtcttc gatgataaaa actttataca tgtttgtttc ctccaattgt tttgggagat
    12541 agagggatca tcagtgtgac agttgttcct tctcggtatt tgctgtcaat cgtgataggt
    12601 ttttgatcat ttccgtaata aagaaggaga cgcttgttta cgttgtaaag tccgtagccg
    12661 atactgaaat ctgaatcgat ccctttggct aactctttac gtatttctgc cagtttttct
    12721 gcagggatgc caatcccgtt gtctataact ttgaattgga tcatctcatc aattttgcat
    12781 gcggtaatac gaataaatcc tttgtgacgg acgtacttcg ttccatggta tacagcattt
    12841 tcaatcaatg gctgcaagat catttttaaa atagaaacag gaaaaagtgt ttctggtata
    12901 tcgatcttat agtcaagcat atggccgtat cttattttta agatgactag ataatcattg
    12961 atatggctaa tttctttttc aagaggaatc cagtcttgtc ctttgcttaa agacgtacga
    13021 aaaaagccgg aaagtgcatg ggtcatagtc agagcttctt caaagcagtc ttgttcgatc
    13081 aaagaaataa tggcatccaa actgttataa ataaaatggg gagtaatctg agcttgcaat
    13141 acacgaactt cactttgtgc taaatggtat tgtttcaaag cgttctcccg taaagactgg
    13201 tttagatctt ccgccatttt attcatgcta gcggtaagtg tttgtaattc cagtgtttcc
    13261 ggcagggtta aacgaaagcc aaggttccct gaagaaagtt gttctgacat ctcgatcagc
    13321 tgattgatcg gttcttggac ctttctctct aaaaagcgac gattcttttt cgcatagaaa
    13381 aaaatggtac taagaactcc aagttccact attgttaata gaatgagcga gcggacaagt
    13441 gtcgtatttt ttttgcttgc taagtcgatc tccacacgaa caaaatcttg taagatatct
    13501 gacagcagtt gagtgacaga atctacctga ttgatgatct cttcattggt atccataaaa
    13561 tcttttgcca agatattatt gatcagctcg ttttgataat cttccagtgt atcgatcgta
    13621 cgtagcgata catctaacgt actaatttct ttaagtgtat ttgtattatt tttgatctct
    13681 cgaatatcat tcctaagttc ttctaggata ttttcctcat tgaactcatc tacggttact
    13741 tggccagaaa tcaagtccca catactttcc aaaacttttt gatcaacctt caaagcaatt
    13801 gcatctgctt tttggatatt tgcgatcgtc tgttgataaa tcaaaagctg ttgcgaatat
    13861 aacagtatgc tcagtagcaa agggataaca gcaaatagaa gcatggcttt gtttgcttgg
    13921 gcaaaaatct gttttatgct taaatttttt gtcatgatac ttctccagtc aataatttct
    13981 ggttgggatg ttagctatat tctgctggga aaagacagtc tcggaaataa aaatatcttt
    14041 aggtaatgtt tttcctgttt gaatatttcc aaaataacga gtaacggtat tggctacata
    14101 ccagccgaca tcaggtttac actcgactac acaattgacg attcctgagc gtagcttctc
    14161 gatcatatct tcttgtccat caatagttac aatgatcaaa tcttctccag gtttgattga
    14221 tgtttctttt atcgcagcta aagcaccgtg agtcatttca tcactatggc tgtataatac
    14281 atcgatttcg gataaatcat ttgtttctaa atatcgtttc atctggtctt ttcctttcat
    14341 ccgaataaaa tcaccgacaa ttcggtgaac aatagtcatc ggaggtttat ggtcgatggt
    14401 tccatctttt tttagggttg gtttacgatt gattgattcc aaaaatcctt cggagcgtag
    14461 cttggtcggt gtggagttct ctgaaccagc aagttctagg attctgattt cctgtttctt
    14521 tgaatttgtg aagtgattgc tgacaaataa acccgcccga tttccttctg ctttgaaact
    14581 ggggccaatg tgggtgacga aaagctcggg atcattcgtt tgaatatgtc tgtcgacaag
    14641 tatgacaggg atccctgctc gctttgcttc ttttaataca gagtcccacc cggattcttg
    14701 caaaggagta aaaatgatca tatccacttt ataggcaatg aacgtccgta tatcttggat
    14761 ttgccgttcc tgattcatgt agccatttcg gtagagaact tggtaatctt ctttttcaag
    14821 cgcagtgata attgattcag tatgcttttt ccgccagcta ctctccgttc ctgattgcga
    14881 aaaaccaacg actagtgggt caggtgcttc ctttgtatta ggtgtacacc ccatcatttg
    14941 gtaagcgatt acaacaaggg ctaaaaaaaa taaaacacgt ttcactttaa atcactccat
    15001 ctggatttgt tagtactatc ttatcagata aaagggaaaa tcaaagtcaa aaggaatcaa
    15061 tcgaaaaaaa tccaagaaac ttaaaaaaat ccaagacaaa aaaagtacac ctaaaaaatt
    15121 taaagtaaat cccataaaaa aatcaatgtt aacgctttct ttattagcta tactcggtgt
    15181 atcaattgaa atgggggata aaaaatgaaa aacaaattta gaaagattgt tgggactatc
    15241 gctattttga ccgcaagcat tctattaagt gcgtgtggca attcaggatc tgcagatgat
    15301 agtacggggt atgtaggaat cgctatgccg accaaatcag cagaaagatg gatcgctgat
    15361 gggaacaaca tggtctcgga attagaaaaa ctgggttata aaacagatct tcaatatgga
    15421 gaagataaag tagaaaatca agtagcacaa atagaaaata tgattacaaa aggggtagat
    15481 acacttgtga ttgcttctat cgatggttcg gctttaactg atgtgttagc taaagcaaaa
    15541 gaagcagata tcaaggtgat cgcctatgat cgtttactga tgaattcaga aaatgtcgat
    15601 tattacgcga cttttgataa ttttggagta ggtgtatcgc aagcagctta tattgaagag
    15661 cacttaggat tgaaagaagg aaaaggacca ttcacgattg aattatttgg tggctcgcct
    15721 gacgacaaca acgcactaat caattacaac ggagtgatgt ctgttttaca accatatatg
    15781 gacaacggtc agttggtcgt gccatctgga cagacgagtt tcagtcaaat cgctacgctt
    15841 cgctgggatg gttctacggc gcaagcaagg atggataatc tgttaagcgc aaattatact
    15901 gaccaaacat tggatgctgt tttgtcgcca tatgatccaa taagtttagg aatcatttcg
    15961 tcattaaaag gcgtggggta tgggagtgaa agtaaacctt tacctgtgat tactggacaa
    16021 gatgcgacag ttgctggcgt taaatcgatc attgccgggg aacaaacaca aacgatcttt
    16081 aaagacacac gtatcttagc aaaaaatacg attgaaatga tcaaagcaat cagtgatgga
    16141 gaagaggttc cggtcaatga tactgaaact tatgataatg gggtgaaaac tgtccctacg
    16201 tatttagcaa atacagtatc tgtcgataaa gataattatc aagcagagct gatcgacacc
    16261 gattattata aagaatccga tctgaaaaat taagaggaac caaacaagga actaaagaga
    16321 tctaagtcag agagaggata agaattatgg cggattgtat tttaaaaatg gcagatatca
    16381 ccaaaaaatt ttctggaatc cgagcgttag attgcgtgaa tctgatgatt gaacgtgggg
    16441 aaatccatgc attgtgcgga gaaaacggag cgggaaaatc aacattgatg aatgtcttga
    16501 gcggcttgta tccatatggc tcctatgacg gggaaatctt ttatgatgga aagctgtgtc
    16561 aatttaaaga tttgaaagat agtgaagaaa aaggcatcgt catcattcat caagaattag
    16621 cactaagtcc ttatctttca ataaaagaga atatttttct cggaaatgaa caagcagaaa
    16681 gaggcgtgat tgattgggaa ctgacagaaa agaaaacata tgaactgctg aataaagtcg
    16741 gtttgtctat cgacccgaat cttctagtat cccagattgg cgtagggcaa cagcaactag
    16801 ttgaaatagc taaagcatta tcaaaatccg tccgtctgtt gattttggat gagcctaccg
    16861 ctgcattgaa tgaagaagaa agcgcgaatt tgctgagatt gatccgagag ttgaaagagc
    16921 aaggtgtaac ctccatcatt atttcacata agctgaatga aatcgtggat atcgcagatc
    16981 gaatcaccat tttacgggat ggacagacga ttgagacact agaaaatgaa atgatcagcg
    17041 aagagcatat tattcgcggg atggtgggca gagatttagt tgatcgttat cctgaaagaa
    17101 aaccggatat tggagaagtt tattttaaag tagaagactg gacggtctat caccctcttg
    17161 atcacgagcg aatcataaac gaccacatca acttgcattt gaaaaaagga gagattgtag
    17221 gaatagctgg actgatggga gcgggcagaa ctgaattcgc tatgagtcta tttggacatt
    17281 cttatggaag caagatatcc ggacgtatat taaaggaagg ggcagagata tcagttaaga
    17341 ccgttccttc agccattgca aacggactcg cttatgtctc ggaggaccga aaatctttag
    17401 gactgaatct gctgatggat attcgggaaa acgtgacgct gagcagtttg gataaaataa
    17461 gtcaaaaagg agtattagat aaagaaaaag aagtaactga ggcagaaaat ttccggaaaa
    17521 agatgcgtat aaaaacaaac tccatccatc aaattgtcag tagtcttagt ggtgggaatc
    17581 aacaaaaagt cgttttatcc aagtggctga tgacacaacc cgatattctt tttttggatg
    17641 agccaacccg tgggatagat gttggggcaa aatatgagat ctacacgatt atcgaagaaa
    17701 tggcagcaaa tggcaaagct gtctgtctta tctcttctga acttcctgaa atattaggga
    17761 tgtgtgatcg aatctatacg atgaacgaag gacgtattac aggagaagtc tcgcgagaag
    17821 atgcagatca ggaagtcttg atgaaattaa tgacgaaaga agaggtgggt taagttggaa
    17881 caggcaaaaa atcttacgga taaaaaagag aaaaaactaa atttgaaaag tagagcagct
    17941 gatctttttg gtcgctacag catggtgatc attttagcgg gactacttat tgcgtttcaa
    18001 ttgatgacgg atggtatttt ctggcgtcca ttgaatataa ctaaccttgt tctgcaaaat
    18061 agccatatat tagtactagc tgcagggatg ttattagttg tgctcttagg tcatgtggat
    18121 ttgtcggtag ggtcagtgat ggcgtttgtt ggtgcaattg cgggcatgat gatggtcaat
    18181 cgccaaatga acccgtggat gacagttatc gcatgtcttg cagtaggcgg gctgatcggc
    18241 gcatggcaag gtttttgggt agcttatgca gggataccgg cgtttatcgt gactttagca
    18301 ggattgctga tgtttcgtgg actgacacaa gttgttctag gaggccaatc tctagcacct
    18361 tttccagctg ttttccaaaa aatgtctacg ggttatttac cagacttaag cgaaggaagc
    18421 agccttcact tcttgacatt gattttagga atgatcatca cgatcgtgtt agttgttggt
    18481 caatggcgaa caagaaatcg tcggaaaaat catttatttg aagtggaatc tccaacagct
    18541 ttcttggtaa aggcggtatt tacagcagtc gttgtactag gaataactta tatctttgcc
    18601 tcttatcaag ggtatccgat tattttgatc attcttggtt tgatcgttgc agcatatggg
    18661 tttttgacaa ataaaacagt agcaggtcga cagatttatg cgacgggcgg aaatcgaaaa
    18721 gcggctgaac tatcagggat caaaacaaag cgaatcacgt tctgggtatt cgtcaacatg
    18781 ggagtcatgg cagctttagc tggattagtt ttagctgctc gattgaatgc ggcaacacct
    18841 caagcaggta catcgatgga actggatgca atggcggcag tatattttgg tggtgcttcg
    18901 acttccggcg gcattggaac aatcatgggt gcgattgtcg gtggcttggt catgggtgta
    18961 ttgaacaatg gcatgtcgat tttaggcgtc ggggtagact ggcagcaggc aatcaaagga
    19021 ttgatcttgc ttttagccgt tgtgctagac ttgtataata agaaaaaacg aacaacgtag
    19081 gagaacgccg gttattcaga aaaaagactg tgacaagaca tttgtcacag tctttttttc
    19141 gctaaaagcg aacattcatt gtttcgtttg tatagactaa aaataatata tgttttttta
    19201 ccattcgtat aaattctctt tttgctcttg atttttttgc ttaattgagt taaattacat
    19261 agggcgctaa aaaaagcctt gattaaatta ataatgttcg tgttttgctg gatgtgaaca
    19321 tcaggaggat aatgtaatga aagtatttga ttacgaagat attcaattaa ttcctaataa
    19381 atgtatcgta aatagccgtt ctgaatgtga tacaacggtc acattaggta aacatacatt
    19441 caaaatgcca gtagtgcctg ccaacatgca gacgattatt gatgaaacaa tcgcagaatt
    19501 cttagcggaa aatggttatt tctatatcat gcatcggttt gatgaagcag ctcgaattcc
    19561 gtttatcaaa aaaatgaaaa agcgtggatt gattacctcg atcagtgttg gcgtcaagaa
    19621 agaggaatat tcctttatag agaagttggc agaagaatcg ctaaatcctg attatatcac
    19681 tatcgacatc gcgcatggac atgctaactc agttattgat atgattcagc atattaaaaa
    19741 atatttgcct gaaacttttg tgattgccgg taatgttggt acaccggaag ccgtccgtga
    19801 attggaaaat gcgggcgcag atgcaacaaa agtcgggatt ggtccaggaa aagtatgtat
    19861 tacaaaaatc aagacaggct ttgggactgg aggctggcaa ctggcagctc ttagatggtg
    19921 tgcgaaagca gctcgcaagc cgatcatcgc tgacggtggg atcagaacac atggcgatat
    19981 tgcaaaatct gtccgttttg gtgcgacaat ggtcatgatt ggttcattgt ttgccggtca
    20041 cgaggaatct cctggcgaaa caaaagtcga aaatggaatc gtgtataaag aatactttgg
    20101 ttctgcttca gaattccaaa aaggcgaaaa acgaaatgtt gaaggaaaga aaatctggat
    20161 ccaacacaaa ggatctttga aagatacact agtggaaatg caacaagacc ttcaatcatc
    20221 tatttcttat gctggaggaa gagatctgga agccattcga aaagttgact atgtcatcgt
    20281 gaaaaattcg atttttaatg gagacacaat ctaaaaaata aataaaatat taggatgttg
    20341 tgaaaaagtt gtcctgcatt aagtaataag aacagccaaa caaaaatagt tctttatatg
    20401 ttttgtttgg ctggaattat tatcgcagat acaagctttt gaacatcatt attcagaata
    20461 agggactgtg ataagatttt tgtcacagtc ccttatgttt tgcaaaaaaa gtttttcttt
    20521 tttctcgtaa attcggtaaa atcaaaagaa aaaatagaca atagaaaaga gaagcttatg
    20581 tgggaaaaag tagagaaaaa acaaggaccg atttatcgtc aaattatgga acagattatg
    20641 agagaaatcg aaaacggaag gatcaatcct ggaagtcaac ttgaatcaga acgaaaacta
    20701 gggcaacttt ttggtgtgaa tcgttccacc gttgtccatg cattggaaga gcttagggag
    20761 cttggggtac taacgagcaa acgtggcagc ggatggtttg ttaatcagac tgaatggggg
    20821 aaattcgctg ttccaagaat cgattggcgg caaatgatct ctcctcgtta tgagcagacg
    20881 gactggtatg agcaaaaaat aagagaagca aagcagatcc aaaaaacatc gtttcttgat
    20941 ttatacgcta gcgagatgcc agatgagctt ttgccgaatt ttgagtttcc ttcttattct
    21001 cttgaagaaa tcttagcgga agaaaaagaa atggatcttt taggctatac tccgttacgt
    21061 aacaaaatca aacggtattt ggaaagtaaa tttgaattgg cattttcccc caaccagctt
    21121 cttgtgactg gtggcggtca gcaagcaatc tttttgattt tacaaacgat tctttcagct
    21181 ggagagggga tagctgtaga gtcaccttct tttttttacc gattacctct tttcaaagct
    21241 tcaggaacgc gtttatttgg gataccgatg gatgaagaag ggatcgactt agaaaaactg
    21301 aaagaatcga tccataaaaa taaattgaaa gcagtgctta tcaacccgaa ttttcaaaac
    21361 cctacaggaa aagtgatgag tcaaaaacga cgagaaaagc tagtagaatt gtgtcgacag
    21421 tatcggcttc caattattga agacgatgta tttacggatc tttcatttct aaaccaagaa
    21481 gaagtccttc ctatacgaac gattgatccg gataatgtgt tatacgtcgg ttcgttatct
    21541 agggttttag gaaaaacaac gaaaatcggt tgggtgatag gacctgagac ttttatctcg
    21601 caactagctc aagcgcaaga aatgatggag ttttcgatga atatccttac acaaatcaca
    21661 gtggctaatg tatttaatga agcaattgat ttgaaaatgc aagatgtaag aaaaaagttg
    21721 aaagaaaaaa gccgactgat aagcgcttgg ggagattcac aatcgttttt caagcttcat
    21781 tcaattttag gtggttacta tgcctggatt acttgggatg ggaaaaaact agaccccgaa
    21841 ctagcagaaa aagtagtaga tatgggtctt ggaattgcac caagtctact ttttgggcaa
    21901 gatataaatg gaatcagaat caattacagt aggctggatg aaaaccaaat accgttgttt
    21961 tattcaaaga tgaaaattct agaaaagtgg ttaacagaat aatttcacaa agtaaataga
    22021 ttaaagaata taacagcttg gatttaaatt tcaagctgtt tctttttttt cggaaaaaga
    22081 atacgatata aagaaactaa gaaaaaacca ttatttgtac gtccgttcac gatgtgtaaa
    22141 taaaataaat gtttgcaaca aaactgtata acaccaacgt ttttaaatag aaacagatgg
    22201 ttgtaagttt gattagaaaa tagaaaaaat gcagatgaaa aataaaacgc tttccttatt
    22261 gtgattttat tcactaatgc tgttaatatg cggattggca taatttcctt tcatcgtgaa
    22321 aatttgtgct tttagcttgt atttctcata aatccatgat aatttattaa tgaagacggt
    22381 taaatagtga tacagtttta ttcactgttt caatcgcaga atgaagagat tttcttatag
    22441 atgagagagg aatgtgtgac aaatggcaaa caaaaaagca ccgatcgact tccaagcact
    22501 gctagatgaa gtcaatgcag atttcccagt atatcaagca cttgatcaag atggaaacgt
    22561 agttaacaaa gatttagtac ctgatttatc cgatgatgaa ctcgttgaat taatgactca
    22621 tatggtatgg tctcgtgtat tagaccaacg ttcaacagcg ctgaaccgcc aaggacgcct
    22681 aggctttttt gcaccaactg ctggacaaga ggcaagtcag ctggctagtg cttacgcatt
    22741 tgataaagaa gatgttcttc ttccaggtta tcgggatgta cctcaactag taaaacatgg
    22801 tttaccttta tctcaagctt ttttatggtc tcgaggacat gctgcaggta attcttatcc
    22861 ggaagaattg aaagctctac caccacaaat tatcatcggt gcacaatatg tacaagcagc
    22921 cggtgttgca ttaggtttga agaaacgaaa caagaaaaat gtcgtcttca cttatactgg
    22981 tgatggcggt tcatcacaag gtgattttta cgaagcaatc aatttcgctg gcgcttacca
    23041 tgcaaatgcc gtattttata tccaaaacaa cgggtatgcg atctctacac ctcgtgaaaa
    23101 acaaactgca gcaaaaacac ttgcacaaaa agctgtcgca gctgggattc ctggtattca
    23161 agtcgatgga atggatccgc ttgcagtata tactgtatct aaaatggcac gggaatgggc
    23221 gatcagtgga aatggaccag tcctaatcga aacactgact tatcgttatg gaccacatac
    23281 actatctggt gacgatccaa cccgctaccg tacaaaagac acagatgacg attggcagaa
    23341 aaaagatcca ttgatccgct tccgtaaata tttgacggaa aaaggacttt ggtctgaaga
    23401 aaaagaagaa caagtcatcg aagcaactaa agaagaaatc aaagcagcta ttgctgaagc
    23461 cgacaaagta ccaaaacaaa aggtttcaga tttcttgaaa aacatgttcg aagtatctcc
    23521 acaatcaatc aaagaacaaa ttgcaattta tgaagcgaag gagtcgaaat aatcatggca
    23581 caaaaaacaa tgatccaagc aatcacagat gccttagcac ttgaactaga aaatgacgag
    23641 aatgtcgttg tttttggtga agatgttggg aaaaacggag gagttttccg ggcaactgaa
    23701 ggattgcagg aaaagttcgg agaagaccga gtattcgata ctcctttagc tgaatctgga
    23761 atcgcaggat tgagtttcgg tcttgcgtta gaaggatttc gtccagttcc tgaaatccaa
    23821 ttctttggtt ttattttcga agcaatggac gaagtagtag ctcaaatggc tcgtacaaga
    23881 tatcgaatga gcggcactag aaatctaccc atcacaattc gttcgccatt tggtggcggt
    23941 gttcatacac cagaattgca ttcggataat ttagaaggtt tgatcgctca gtctcctggt
    24001 attcgagtag tgattccttc caacccttat gatgcaaaag gactattgat ttcttctatt
    24061 agaagtaacg atccggttgt tttcctagaa cacatgaaac tatatcgctc attccgagaa
    24121 gaagtgccgg atgaagctta cgaagttcct ttggataaag cagcagttac tcgtgaggga
    24181 acagatgtat ctattatcac atacggagca atggtgcgtg aagcaatcaa agcagcagac
    24241 aatcttgcaa aagaaaatat atctgttgaa atcattgatc ttcgtacagt tgcgccatta
    24301 gatgtagaaa cgatcattca atccgtggaa aaaactggtc gcgtagttgt tgtccaagaa
    24361 gcacaacgac aagcgggcgt tgccgcgcaa gtagtttctg agatctcaga acgcgctatc
    24421 ctttcattag aagccccaat cggacgagtt tctgcacctg atacagtctt cccatttgga
    24481 caagcagaaa atgtctggtt gccaaatgca aaagatatcg aagacaaagt aaaagaaatc
    24541 gcggaatttt aagaaaagaa gggacgaaac aaagtcatgg cttatcaatt taaattgccg
    24601 gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat ggttcgtcaa accaggagat
    24661 acgatcaatg aagatgatac attactagaa gttcaaaatg acaaatcagt agaagaaatc
    24721 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
    24781 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
    24841 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
    24901 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agttaccgga tatcggtgaa
    24961 ggaatcgcag aaggtgaaat cgtcaaatgg ttcgtcaaac caggagatac gatcaatgaa
    25021 gatgatacat tactagaagt tcaaaatgac aaatcagtag aagaaattcc gtcacctgtc
    25081 acagggacag ttaaaaacgt gattgttcca gaaggcactg tagcaaatgt tggggatgta
    25141 ttagtagaaa tcgatgctcc tggacacaat agtgcaccgt caactagtgc gccatcagct
    25201 gaagcaccaa aagaaaaagt ggaaacatca ggttcagcaa gtgtcgttga agctgccgat
    25261 ccaaataaac gtgtattggc tatgccatct gttcgtcaat ttgcccgtga aaaagatgta
    25321 gatatctcac aagtaactgc aactggaaaa ggtggcagag taacaaaaga ggacatcgaa
    25381 aacttcttgg caggtggtcc aagcagtgca ccagcgaaat cagaagcgcc agaagcggca
    25441 gcaccgaaag aagcagctcc tgcagcagaa agcaaaccag ctgcaccagc aaaaccattc
    25501 aagtccaact taggagactt ggaagaacgt gtagcaatga cgccaacacg taaagctatc
    25561 gctaaagcaa tggtcaacag taagcataca gctccacacg tgacattgca tgatgaagta
    25621 gaagtgtcta aactatggga taatcggaaa cgatttaaag aagttgctgc tgcaaatggt
    25681 acgaaactta ctttcttgcc atatgtcgtg aaagctttaa cagcgactgt taagaaatac
    25741 ccagtattga atgcatctat cgacgatgca aaccaagaaa tcgtttataa acattactac
    25801 aatatcggta ttgcaactga tacggatcac ggactatatg taccaaatgt gaaagatgca
    25861 gaccgcaaag gaatgtttgc tattgcagac gaaatcaatg agaaagcaaa attagctcat
    25921 gacggcaaac tatctgcaga agatatgcgc aatggaacaa ttacgatcag caatatcgga
    25981 tctgtaggtg gcggttggtt tacaccagtc atcaactatc cagaagtagc tattttaggt
    26041 gtaggaacga ttgcacaaca accaatcgta aatgctgaag gcgaaatcgt tgtcggtcgt
    26101 gtgatgaaac tgtcattaag tttcgatcac cgtatcgtag atggagcaac tgcgcaacaa
    26161 gcaatgaata atatcaaacg tcttttagct gatccagagc tattaatgat ggaaggatga
    26221 taaacaaatg gtagttggag attttgctgt agaattagat acagttgtaa ttggtgcggg
    26281 acctggtggc tatgtagctg ccatccgcgc tgctgaaatg ggacaaaaag tagcaatcat
    26341 tgaaagagag tatattggcg gtgtttgttt gaatgtcggc tgtattcctt caaaagcact
    26401 gatagctgca ggacaccatt atcaagagtc actagattct gaaatgtttg gagtaactag
    26461 tgaaaacgtc aaactagatt ttgcaaaaac acaagaatgg aaagaaaaca aagttgtcca
    26521 tacgttgact tctggtgttg gtttcctatt aaaaaaacat aaggttgaaa caatcgaagg
    26581 agaagcgttc ttcgtagatg atcacacact acgcgtcatt catccagatt cagctcaaac
    26641 ttattcgttc aataacgcaa tcatcgcaac aggttcccgt ccaatcgaga ttccaggatt
    26701 taaattcgga ggacgtgtgt tagattctac tggcggatta gcattgaaag aagtacctaa
    26761 gaaatttgtg atcatcggtg gcggtgtgat tggtgccgaa ttgggcggag cgtacgcaaa
    26821 cttaggagca gaagtaacga ttcttgaagg ctcaccacaa attttaccaa cttacgaaaa
    26881 agatctagta aaattagtag aagatgattt caagaaaaaa ggcgtaacag tcgtaacgaa
    26941 tgcaatggcg aaagaagcag tcgataacgg tgacagcgtg acagtgaaat atgcagtgga
    27001 cgggaaagaa gaatccgtta ctgctgatta tgtgatggtc actgtaggtc gtcgtccgaa
    27061 tacagatgat atgggcttag aacaagcggg tgtcgaagta ggagaacgag gattgatcac
    27121 ggtggataaa caaggacgta cgaacgtacc taacatctat gccatcggtg atatcgttcc
    27181 aggggcggca ttagcacaca aagccagtta tgaagcaaaa atcgctgctg aagcaatctc
    27241 tggcaaaaaa gtagctgtag attataaagc aatgccggca gtagccttca ctgatcctga
    27301 attggcttct gtaggtatga cgatcaaaga agcaaaagat gctggtatcg aagcaaaagc
    27361 atataaattc ccattttcag ggaacggtcg ggcattatcg cttggtaaaa cagaaggatt
    27421 catccgtcta gttacgacga tcgaagataa cgtgcttatt ggcgcgcaaa tcggtggagt
    27481 cggcgcaagt gatatggtct ctgaattagc tttagcaatc gaatcaggaa tgaatgctga
    27541 agatatcgcc ttgacgattc atcctcatcc atctcttggc gaaatcgtca tggatgcttc
    27601 ggaattagca ttaggtttac caatccatat ttgatttgaa taatacaggc gaaacgcaac
    27661 tgaaaaatca gttgcgtttt gttttttaga attatcaata atattattat gtaaacttaa
    27721 aaatatgaca acttcttggt ttattaccac ttcctatgct acactgaaga ggaatatgaa
    27781 ataggagagt tgaagatgca agaatatcag tatccacttg atttagattg gacaacagac
    27841 gaaatggtca ttgtaatgaa gatgtgggaa gatctggaac gtgcgaatga aaaagggata
    27901 aacaatgaag cttttttgaa gacttatcag cagtttaaaa ctgttgtcaa atccattggt
    27961 gaggaaaaac gattgggacg ggaatttgaa aaagcttccg gttattcttt atatcgtaca
    28021 gtgcagcaag caaaaaaaaa tccaaacaaa actttgagaa tgaacgggtg agataaatgg
    28081 aacagatgat tgatcatatc aagtcctggc ttgaagaagc tggtgagaaa atcaaatctt
    28141 ctcaacaaac attaacaatc tcgcaaaaat caaatcgaac ggatctagta acaaatgtcg
    28201 atcgaaacat ccagaatttt ctaatcgaaa aaatccattc ctttgatcca gaggcgaaaa
    28261 tattggcaga agaagaaggg tgtaatcaat tatcggatct cgatggaaga gtctttatca
    28321 tcgatcctat tgatggcacc ttgaattttg tgattgaagg ggaaaatttt tgtattatgc
    28381 tcgctgttta tgaatccggc gttggaaagc ttggtttcat ttatgacgtg atgagaaatg
    28441 aacttttttg gggtggaaaa ggaataggcg tgtataaaaa tgatgagcca ttgcctagac
    28501 cagcaaataa atccttatcg gaaggcttac ttggtgtgaa tagttatctt ttcggtcata
    28561 ataaatatgg aatccgagaa attgcggaaa gcagcatggg gacacgtatg tacggatgcg
    28621 ctggtcttga actagtggca atcttgaaag gaaaccacat tgcttatatt tcaaacctta
    28681 gcccttggga ttatgcagcg ggaaatgtct tattggaaga gtttggtatg gtaacaagcg
    28741 gtctttttgg tactcctttg acatttgaaa aaagggaata ctatttagca gggacttctt
    28801 gtgcatatgc agaaatcgca gaccttttga aaaaagaagg ctgaaaaaaa ggtagaaatc
    28861 attcacaaaa atgataaaaa cgagcaaaag attaattttc tcgaaaaaat gaaaacattt
    28921 tactatattt catgaaaaaa gtctgctata attagcagtc gagtaaaatt tgccctgtca
    28981 taaagaacca ataaaaaagg agttagttac attgaattat agaaacgata tccgtaacgt
    29041 cgcaattatt gcccacgttg accacggaaa aacaacatta gttgatgaac tattaaaaca
    29101 atcagataca ttagacgcac atacgcaatt gcaagaacgt gcgatggact caaatgcgct
    29161 agaaaaagaa cgtggtatca cgattttagc caaaaacaca gctgtagact ataaaggtat
    29221 tcgcgtaaac atcatggata ctccaggaca cgcggacttc ggtggcgaag tagaacggat
    29281 catgaaaatg gttgacggcg ttgtgttggt cgtagatgcg tatgaaggaa caatgcctca
    29341 aacacgtttt gtattgaaaa aagcactgga acaacatatc acaccaatcg ttgtcgtaaa
    29401 caagattgac aaaccttctg ctcgtcctga gcatgtcgta gatgaagttc ttgaactatt
    29461 catcgaatta ggagcagatg atgatcagtt agactttcct gtgatttatg catctgcact
    29521 aaatggtacg tcaagcttgt cagatgatcc agcagatcaa gaaccaacca tggcacctat
    29581 ttttgataca attattgaaa aaatccctgc tcctgtcgat aacagcgacg aaccattaca
    29641 attccaagtt tcattacttg actacaacga ttatgtaggt cgtatcggaa tcggacgtgt
    29701 atttagagga acaatcaaag taggagacca agtagcatta attaaattag acggaacagt
    29761 taagaaattc cgtgtaacga aattatttgg tttctttgga ctaaaacgtc tagagattca
    29821 agaagcaaaa gctggagact tgattgctgt ctcaggtatg gaagatatct ttgtcggtga
    29881 aacagttact cctgtggatc atcaagatgc gttacctatc cttcacattg acgaaccaac
    29941 attgcaaatg actttcttag ttaataattc accatttgcc ggtcgagaag gtaaattcgt
    30001 aaccgctcgt aagattgaag aacgtttgat ggcagaactt caaacagatg tatccttacg
    30061 agttgaacca acaaactctc cagatgcatg gacagtttct ggtcgtggag aattgcattt
    30121 gtctatcttg attgaaaaca tgcgccgtga gggatacgaa ttgcaagtat ctcgtccaga
    30181 agttattgaa aaagaaatcg acggtgtaaa atgcgaaccg tttgaacgcg tgcaaatcga
    30241 tacaccagaa gaatatatgg gtagtgttat cgagtccttg agcttacgta aaggtgaaat
    30301 gcaagatatg gtccatacag gaaacggaca aatccgtttg acattcttaa cacctgcccg
    30361 cggtttgatt ggttattcaa ctgaattcct ttcaatgaca cgtggttacg gaatcatgaa
    30421 ccatactttt gaccaatatc tgccaatgct tcctggtcaa attggcggac gtcaccaagg
    30481 ggcgcttgtt tcgatcgata caggaaaagc aacgacttat tctatcatga gtatcgaaga
    30541 acgcggtaca gtcttcgtag aaccaggtac agaagtatat gaaggaatga ttatcgggga
    30601 aaacagccgt gataatgatt tgactgtcaa catcacgaaa gcaaaacaaa tgacaaacgt
    30661 acggtctgct actaaagacc aaacatctgt catcaaaaaa ccaaaacaat tgacgttgga
    30721 agaatctttg gaattcttga atgatgatga atattgcgaa gtaacaccag aaagcattcg
    30781 cttgagaaaa caaattttag agaaaaatgc tcgtgaaaaa gcatctaaaa agaaaaaata
    30841 atctgtaaaa ggatgaagag agaaaccagc ttgttataaa aaatagctgg tttctttttt
    30901 tg
//
LOCUS       DACDMM010000017        30717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDMM010000017 DACDMM010000000
VERSION     DACDMM010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.017"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            137..1423
                     /gene="efmA"
                     /locus_tag="IWD16_000587"
     CDS             137..1423
                     /gene="efmA"
                     /locus_tag="IWD16_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP9087671.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1561..2160)
                     /locus_tag="IWD16_000588"
     CDS             complement(1561..2160)
                     /locus_tag="IWD16_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP9087672.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2564..3685
                     /gene="mnmA"
                     /locus_tag="IWD16_000589"
     CDS             2564..3685
                     /gene="mnmA"
                     /locus_tag="IWD16_000589"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP9087673.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3931..5352
                     /gene="arcD"
                     /locus_tag="IWD16_000590"
     CDS             3931..5352
                     /gene="arcD"
                     /locus_tag="IWD16_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP9087674.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5366..6484
                     /locus_tag="IWD16_000591"
     CDS             5366..6484
                     /locus_tag="IWD16_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP9087675.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6544..7836)
                     /locus_tag="IWD16_000592"
     CDS             complement(6544..7836)
                     /locus_tag="IWD16_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9087676.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7986..8546
                     /locus_tag="IWD16_000593"
     CDS             7986..8546
                     /locus_tag="IWD16_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9087677.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8675..9052
                     /locus_tag="IWD16_000594"
     CDS             8675..9052
                     /locus_tag="IWD16_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP9087678.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8985..11057
                     /locus_tag="IWD16_000595"
     CDS             8985..11057
                     /locus_tag="IWD16_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9087679.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11268..12215)
                     /locus_tag="IWD16_000596"
     CDS             complement(11268..12215)
                     /locus_tag="IWD16_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9087680.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12461..12925
                     /locus_tag="IWD16_000597"
     CDS             12461..12925
                     /locus_tag="IWD16_000597"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP9087681.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(12987..14030)
                     /locus_tag="IWD16_000598"
     CDS             complement(12987..14030)
                     /locus_tag="IWD16_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP9087682.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(14407..15372)
                     /locus_tag="IWD16_000599"
     CDS             complement(14407..15372)
                     /locus_tag="IWD16_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP9087683.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPCLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            15606..16451
                     /locus_tag="IWD16_000600"
     CDS             15606..16451
                     /locus_tag="IWD16_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9087684.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            16642..17829
                     /locus_tag="IWD16_000601"
     CDS             16642..17829
                     /locus_tag="IWD16_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9087685.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            17835..18191
                     /locus_tag="IWD16_000602"
     CDS             17835..18191
                     /locus_tag="IWD16_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP9087686.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            18193..18531
                     /locus_tag="IWD16_000603"
     CDS             18193..18531
                     /locus_tag="IWD16_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP9087687.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            18926..19960
                     /locus_tag="IWD16_000604"
     CDS             18926..19960
                     /locus_tag="IWD16_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9087688.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            19953..20639
                     /locus_tag="IWD16_000605"
     CDS             19953..20639
                     /locus_tag="IWD16_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9087689.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            20663..21511
                     /locus_tag="IWD16_000606"
     CDS             20663..21511
                     /locus_tag="IWD16_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9087690.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            21708..22481
                     /gene="sufC"
                     /locus_tag="IWD16_000607"
     CDS             21708..22481
                     /gene="sufC"
                     /locus_tag="IWD16_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP9087691.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            22494..23780
                     /gene="sufD"
                     /locus_tag="IWD16_000608"
     CDS             22494..23780
                     /gene="sufD"
                     /locus_tag="IWD16_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP9087692.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            23777..25012
                     /locus_tag="IWD16_000609"
     CDS             23777..25012
                     /locus_tag="IWD16_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9087693.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            24999..25469
                     /locus_tag="IWD16_000610"
     CDS             24999..25469
                     /locus_tag="IWD16_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP9087694.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            25474..26865
                     /gene="sufB"
                     /locus_tag="IWD16_000611"
     CDS             25474..26865
                     /gene="sufB"
                     /locus_tag="IWD16_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP9087695.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(26960..28102)
                     /locus_tag="IWD16_000612"
     CDS             complement(26960..28102)
                     /locus_tag="IWD16_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9087696.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(28141..28875)
                     /locus_tag="IWD16_000613"
     CDS             complement(28141..28875)
                     /locus_tag="IWD16_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9087697.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            29107..29382
                     /locus_tag="IWD16_000614"
     CDS             29107..29382
                     /locus_tag="IWD16_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9087698.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            29480..>30717
                     /locus_tag="IWD16_000615"
     CDS             29480..>30717
                     /locus_tag="IWD16_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087699.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSS"
BASE COUNT        10020 a         5548 c         6457 g         8692 t
ORIGIN      
        1 tttattgttt ctaagtaaag aaatttaaat tgaaacgatt gttatttagg gaaaaaggta
       61 ttttttgaaa aaagaatccc gtataatgat tgttgtgcaa acgctataca aaatgaaaaa
      121 gaaaagaggg atgtttatgg aaaatgaaca gtcggtcgtt ttaacgaatt ggaagagaaa
      181 ttatttgttc tttttatcgg gacagttttt gtcaggaatc actagtatgg tcgtccaata
      241 tgcgatcatc tggtatttga caagagaaac cggttcggcg acaattttga gttttgctac
      301 tttactagga atgattccca tggttttact aagcccattt gtaggaccgc tagtggatcg
      361 ctgggataaa aaagctcttt tgattgtcac ggatattatt gtagcgattt tcgcgcttat
      421 tttagcagta gtcggaacga tttcagaatc cttcccgatt tggctagttt ttgtgtcttt
      481 gtttatgcgt tcggtcgcac aaacattcca aatgccaacg atccagtcga ttatgccgac
      541 aatcgttcca tcttctcaca taacaaggac gaatggacag ttaggaatgg tccaatcagc
      601 gaatttcatc attgcgccag ctctaggtgc ggctttgttt tcagtcgttc ctgtcaatta
      661 ccttatttta ttagatgtat tgggggcagt ttttggtgtc ggcttattga tttttgtgaa
      721 gatcccaaaa gtttctcctg aaatattgga agttccgctt actattttta aagatgcaaa
      781 atttgggctg cagcaattga tggataataa agggttatgg tacataacga tcaatggtgc
      841 atttgtgatg ctgttgttca tgccagcaat cagtctgtat ccattgatga cgctagatta
      901 ctttggtggt tccgttggac aggcgggagc agtggaagta gtctacgcag tcggaatgct
      961 cttaggcggt gcactgatca gttttatagg aacatggaaa gaccgaatga aacctattat
     1021 tatagcctac atcattatgg gcctgacgat cggtgcaagc ggattggttc caaacgatag
     1081 tcaagggttc ttgtactttc ttatcttgaa tgcaggagca ggttgtgcaa cgccatattt
     1141 caatacactg ctgatggcaa tgatccagca gagttatgaa tcgaatgttt taggtcgtgt
     1201 cttagggaac ttcaactctt tgatgaacct gacaggtcca attggattat tatttgcagg
     1261 accattagct gatcgtttag gggtagaaaa aatgttcttg ttttccggaa tcgggatttt
     1321 attatgcgga atcgttttat ttttaacgtc agctgcccga aattatgata aagaattaca
     1381 gaaaaaactg gtaaaagaac accatgagca aaaagacgaa tagtatggaa tggagttgtg
     1441 acagaagtct tgttacaact ccatttctga tcctaatgtt cctgtggcaa taagcctact
     1501 caaatgaaaa atatgagtaa ttatctttat ttgtttaata cttgatatag ggcagttttt
     1561 tcacgaccta tcgtacttcc tctttgcttc atagatttct ttgatttgtt gctcagataa
     1621 cggagtgcca aatggtagtt tttcctttaa gagttctctt tccggtgcgt ctacgcctac
     1681 attgacatcg ttctcgatag gtacgctgta atgatggata tgtccatgaa gattgcggat
     1741 agtgtcgtta ggcccgagca acatcggata atgagtcaag taatattgtt gatggtcgaa
     1801 tttaacgatg gcacctacat cataaaaata aaatttggga cttccgtcag taagcctagg
     1861 atcatttttt tctaaataac ggaaaaaagc gcgactgtca tggttccctt tgatcaagtg
     1921 gattgttcct tgcagttgag tgaccagttc caaaatttct gcatttgctt tttcatattg
     1981 cggatgcata gcaaggtcgc ctagatgata aacatggtcg gtttctttta ttactgcatt
     2041 ccagctagtg atcaactgtt ggttcatttc atccacattt gcaaagagcc gcggagcaaa
     2101 gtcgtttttt cctaataagc gttcatggaa aaagtgcata tcactgataa agtaattcat
     2161 gtatcttttg cctccctttt ttcttagagt aaacaaaatc acttatctga gcaactcata
     2221 acatctaaaa agaaaaggtt gtaaatgaga cgtgcagctt taagcgataa gaagcaattt
     2281 tgaaaaatag tttttcacaa tttataaaat tattccttat tgccgaaagg ctggcccttt
     2341 ttatgagtat gccaattttt tatatcgaat gctcaaaaga tgagtgattt tttggttgac
     2401 tatcatgttt gtgtactgaa taggtccagt agaacagtat ttttacgact tgataattaa
     2461 gggatgaaat tttcagaaat atgacgtata atgtaaaata ctgaaaagtt gcgaccttca
     2521 actcgtagat gtttcagaat ctaattgaaa tgatggtgat atcatgacag acaacagcaa
     2581 aacacgcgtt gtcgtaggga tgagcggcgg tgtcgactca tctgtaacag cacttttgtt
     2641 aaaggaacaa ggatacgacg tgatcggaat attcatgaaa aattgggatg acacggacga
     2701 aaacggtgta tgcacggcca cggaagatta taaggatgta gcgaaagtag ctgctcaaat
     2761 cgggattcct tattactctg tgaattttga aaaagaatat tgggatcgtg tatttgaata
     2821 tttcctagcc gaataccgcg caggacgtac acctaaccca gacgtgatgt gcaacaaaga
     2881 aatcaaattc aaagcattct tggattatgc aatggatctt ggcgccgatt atgtagctac
     2941 tggacattat gcgcaagtca ctcgcgatga aaacggcgtg gtccacatgc ttcgtggagt
     3001 agacaataat aaagatcaaa cttacttcct tagccaatta tcacaagaac agctttcaaa
     3061 aacgatgttc cctctaggcg gcatggaaaa atcagaagta cgggcaattg ctgaacgcgc
     3121 aggccttgcg actgctaaga aaaaagattc aacgggaatc tgctttattg gtgaaaagaa
     3181 cttcaaacaa tttttgagca actatctgcc agcaaaaaaa ggaaatatgg tcactcttga
     3241 tggtgaagtc aaaggacaac atgctggctt gatgtattat acgattggtc aacgtcaagg
     3301 actagggatc ggtggcggag gagattcgca agaaccctgg tttgttgtag gaaaagatct
     3361 agcgacaaac acattatatg tggggcaagg gttccatcat cctgcattgt atgccacaag
     3421 cttggatgca agtgaaatcc actttacgac gaatgaacca atgccaaaag aattcaagtg
     3481 tacagcgaaa ttccgttatc gtcagcaaga cgttcctgtg acagttcgct tattggatga
     3541 taatcgtgca gaagtcgtat tcgacgaacc tgtacgtgca atcactcctg gacaagcagt
     3601 tgtattttat gatggaatgg aatgtctggg tggtggtttg atcgaccacg catatcaaga
     3661 aacaaaagtt ttacaatacg tttaagcgat attaaatgaa tcaaaacagc tatggctaac
     3721 aaaagaaaca ccttttcttt tggatagcat agctgttttt ttgcaataaa attaattttt
     3781 attcatttta tacttgaaat acttgtgtaa aaagttcata atatccaata atatgaataa
     3841 agttgctttt tgttaagcaa gagcagttgc aaaatagaag ggaatctttt gcaagtgatt
     3901 tgttcaattt tataagtgag ggtgatggaa atggaaaaga aatcgacggg tattagcaaa
     3961 tggggactgg ttgctctggt tgtcagttcg tcaatcggaa gcggtgtgtt tgggatcaca
     4021 agtgatctgg ctagttctgc agctccaggg ccagcgatcc tttcatggat aatcgttggg
     4081 attggtattt tagctttggt attatcgctg aatcatttag gagaaaagcg tccagatcta
     4141 gatggtggta ttttcggtta cgcggaagct tcatttggta aattaggtgg cttcatcagt
     4201 ggttggggtt actggttgtc tgcgtggctg ggaaatgttg cttttgcaac gatgctgatg
     4261 agcgccatgg gagaattttt cccgatattc aaaggtggac aaaatgttcc ttctatttta
     4321 tttgcaagta tttttatttg gggtctgact ttattagtaa acaatggagt tgaaggagcg
     4381 agcgtcatca atacaatcgt gacgatctgt aaattagtgc cattattcct atttttaata
     4441 tttgtattta tcgcatttaa agtgaatata ttcactgctg atttttgggg taacgtatct
     4501 gataatctag tgaatggaac agggcagcaa ggaacgatct ggttgcagat taaaggttgt
     4561 ttgatggtta tgatgtgggt ctttgtaggg atcgaaggcg caagtgtttt agccaaccgc
     4621 gcgaagaaac gttcagaagc ccagcaagca tcgatcattg gtctgctttg tttattgttt
     4681 atctatatcc ttgcttcgat cttgccatac ggcgtcttat ctcaagaaga gttagcaact
     4741 atttcacaac ctgctatggc aaatatttta aaaggaatcg tgggaaattg gggagcagcg
     4801 ctgatcaata tcggcttgat tatttcgatc attggttctt ggttgtcttg gacgatgctc
     4861 ccagcagaaa caacgatgtt gatggctcga gacggtctgt tgccgaaagg ttggggaaaa
     4921 acgaataaaa aaaatgcccc aacttattcg ttagttgtta ctgcaatctt gactaatcta
     4981 ttcttattga ctttcttagt tactgattat gcctatcaat ttgcctattc tttatgtaca
     5041 gcggcaattt tgatttgtta tctacttgtc gggatctatc aagtccagtt ttctttaaaa
     5101 gaaggaatca aaaaacaagt agtgatcggt gggattgctg tagtttttga attgatgggc
     5161 atcattttag caggattttc ttatgtcctt ttatgtagta ttgcctatct tcctggtttt
     5221 tgcttctact ggatcgcttg tcgtgaaacc aaacatcaaa ttacgggaaa agaaaaagta
     5281 atgattgggc tgatttgtgt ggccgcactg ctttcaatcg ggttgcttgc tgtaggctgg
     5341 atcaagatat aagggggaaa gaaacatgaa aatcgcaaat ttcggtgtag aagaatggct
     5401 aaataaatgg gaaaagaaag cggtatatga tattgcacaa agctcgatcg aagcattgac
     5461 attagacgaa ctcgttggct tagatgggac tagcgtaagt aaatttttcg aaaaacgcaa
     5521 aaacgaacca ttggactatg gctggatcga aggatcagat gttttcaaac aagaagtcgc
     5581 ttctctttat caaaccgtcg atccggaaaa catcttacaa acgaatggag cgactggtgc
     5641 taatctttta gctctatacg cgttagtgga agcaggagac catgttgttt cgatgcttcc
     5701 tacttatcag caattatatg acatccccaa atctttaggt gccgctgttg actttgttca
     5761 tttgaaggaa gaagaagatt ggcagtttga tttagaacag ctagaagcct tagtcaaacc
     5821 tgaaacaaaa atcatctgtt tgaatagtgc aaacaatcct acaggcacat tgctagatcg
     5881 gaaaacttta gaaaaaatcg cagaaattgc tcgtacagta gatgcatatg tattgataga
     5941 tgaagtatat gcgcctttaa cggataaagg tgaattcttg tcgatcgtag atgtctatga
     6001 aaaaggtata gcaaccaatt ccctatccaa aacctactcc attccaggta ttcgtattgg
     6061 ctggacagct actggacctg agttggcaga agtttttcga aagtatcgag actacacgat
     6121 gatttgcggc ggagtcctgt cagatgatct tgctgtacat gcattgaaga ataaagaaaa
     6181 aatattggcg cgaaacaaaa agatcattac agagaacctt gtgattttaa aacaatgggt
     6241 agccaatgaa ccgaaagttg aacttgtcgc tcccaattac gtctctactt ctttcattaa
     6301 attagccatc gaagaagatg accagacatt ttgtatcaat ttattagaag aaacaggagt
     6361 actattagta ccaggaagtg cctttgattt accgaagcat gcccgactag gttactgttg
     6421 taaaaaagaa accttggaaa agggattatt cctcttatct gagttcttga aaaaacaggc
     6481 ctaaacaaaa acactgcaag gacagtctgt gacaactgtt caagcagtgt ttttcattta
     6541 cgcttattgt ctatgagaag gagagtgccc tgaagctatg gagactcctg aagctttttc
     6601 ttctgcagtt tcttgctgat ggatctcttg tgtattgtgt cgtacaagca agaagttgac
     6661 caaaacaaga agagaagtcg aaagaaagac gccacgataa ccaaatgcac tggaaacact
     6721 ggagccgatc ataggcccta ctacgttacc agttgcttgg aaagattggt tataactgaa
     6781 gatccgtcca gcagcttgat gaggcgaata ttttgtgatc aatgcttgga cagcagggag
     6841 cagacaagca tcagagatac caatcaaaaa acgtaaggca gctaattgcc aaacattcgt
     6901 gacgaatgcc attgggatat aaaccagaat cgcaaaaccc aaaccaatcg caagtattct
     6961 ttcactccca atcttgtcac ctaagcgacc aaatcttgga gcggctatca aagtggcaat
     7021 cccaggtatc gaagcaatga ttccgctgat caacgtaaca ttcccatgtc catgcagcaa
     7081 ttggcgaatg tacagactga tgattggact aatggaattg ttggatgctt ggatgatcat
     7141 cgttgtgacg aacataccga tgacgacgtg tggatatttc aagtctttga aaatctgttt
     7201 ggcagaaacc atgttttctt ttttcactgg gacaaatttt tcatggacga aaaacagact
     7261 caacaagaag acgaaaaata agatgatccc tgtaatgaaa aaggtagggc gatagccaaa
     7321 cgctgaagca gagatcccgc caagaagcgg tccaagtaat gttcctgtaa cagaaccagt
     7381 tgctaaggtt ccaagaactt gaccactttt ttcttttggc gtcccagttg cgacaagggc
     7441 agtggcatta ctgatataac cagaaaaaat cccttgaagc agacgcaatg cgactaattg
     7501 atacacacta gtcacgcaac ccatcaaaga aatgacgatt gccattccta atgaagcacg
     7561 caacagcatc aattttcttc ctttttggtc ggctaatttt ccccaccaag gagagataag
     7621 cgtagtcacc aagaaagtag atgaaaaagt caatccgctc caaaagttca attgtgacgt
     7681 actataatta cctaacgtat ctatgtaaag tgacataaaa ggcatgacta aactaaatcc
     7741 gataccagcc ataaatgttc caaaccataa gacaaacaag ttttgttccc aagtttctct
     7801 tttcctaaat aatttttcct ttattcttgc agccaattag actccttctt tctgaaaatt
     7861 ttcatctaca gctctttatt ataaactaca atatgtagtt tatcaatgaa agtctctttt
     7921 ttctctttct ttttcgtggt aaactgaaag agtgaaggat ttattttatg agaggaaaca
     7981 tggaaatgaa aaaaagaaat ctgtctcaag agaaaatcat cgactgcttt cgagaactag
     8041 cagaagagat ggatgttcag caaataacct ttcagcattt agccaaacgc ttggatatca
     8101 aggctccctc gctgtataac catttcaaaa atattcgtga tgtcaaaaca gcgctaacag
     8161 cgaaacttct gcaggaactt aatgaccagc tgcgacgggc tcttgtggga aaaagtggtc
     8221 aggaagcact gcagatctat gcagaaactt atcgatcctt tgctttttca aatcaagcag
     8281 tgtatgagtt actgatcagt gtaccgcata ccaatgaagc tgttttatta gatggaatcc
     8341 atgaaacgaa tcagatcatt ttgcagatat ttgaggcttt tcaattaacg aaagaagaaa
     8401 aagttcatcg aagcagggaa cttcgtagta tgatccacgg ttatctttcc ttacgttttt
     8461 taggatactt tacaaaagaa ccaacaatcg atccagaaga cagctatttt tggatgatca
     8521 ataactttat cgctactttg ccttaaacaa tcagattcat ttgtaacaat tttaagatgt
     8581 tcattaaatg acccttttat aagagaaaat tcttgcaact ttcttgaaaa gtgactaaga
     8641 tgagtctaga aaagctgaaa gaaggagcac gagtatgtat caagtaatta ccatgttcgg
     8701 ggacaatgag ccttggtggt tttttgaaga ctgggaagaa gatattgaag aagaagaaac
     8761 attcgagtct ttcgaagaag cacgacaagc gtacgaaaaa caatggaacg aaatccatca
     8821 aaattatgaa tatatcaatg caaaaagtaa ctttctcagc gccttttgga atgacggaga
     8881 tgagcgctgg tgtgaagagt gtgacgatga tttacagcaa tacaagggac ttgcgttgtt
     8941 gaaaaatcat cagcccatca cagtagaaag cagaaaggaa ttttatgaaa caactaattc
     9001 tagcggaaaa accaagcgtt gcgaaagacc taagcaaggt gcttggtgct aatcaaaagc
     9061 acaaaaatta ttatgaagga cccaaggtga tcgttacttg ggcccttggt catttattag
     9121 gattgaaaat gccggaagat ctgaataagg aatggcagag ctggcaaatg gacacgttgc
     9181 caatgatccc taaaaaacta ggaatcaaac cacttcctaa aacaggacat caattaaaag
     9241 caatcaagca gctggctcag cgaaaagatg tctcagaagc agtcattgcg acagatgccg
     9301 gaagagaagg ggaacttgtt gctcgctgga tcttagagta tgtgcatttc aataaaccag
     9361 tgaagcggtt atggatctct tctcaaacag ataaagcaat caaaactgga ttcaagcaac
     9421 taagacctgc gaaagaatat gatgcattgt atgaatctgc tcttgcccgg gcaaaagcgg
     9481 actggctggt aggattgaat gtgaccagag ccttaactgt gaagtacgcg gataatttat
     9541 cagcaggccg tgtgcagaca ccgacactag cattagtgag agaccaagaa aagaaaatcg
     9601 agcagttccg tccacaaacg tatttcacga tcacgttgca agctggaaaa gaacaagcca
     9661 aaatgcttca aaaaaatcaa tttgctttaa aaacacaaga agaagcacaa aaacttgttc
     9721 aattgatcag tacaagcaaa ggaactgtca cagaagtcca agaaaaatta aaaacggaat
     9781 ctgcgccatt gccttatgat ttaacagaga tccaaagaga agccaatcaa cgctatggct
     9841 attctgcaaa gaaaacatta agtcttgtac aaagtttgta tgagacccat aagatcgtta
     9901 cttatccgcg tacagacagc aaatatctga caaatgacat gaaaagtacg atgaaagaac
     9961 gattgcaagc agtcagcgac tttgctcctg aggtcaagca gtatttgaaa aacggagcca
    10021 tcgtccaaca gacaaaagta ttccaagaca gtaaagtgac cgatcaccat gcacttttgc
    10081 caactgaaaa cagagcacgt tatgaaaaat taagtaatga agagcgaaaa atctatcaaa
    10141 tgattgtctc tcgtttttta ggactgtttg ctcaacccca taaagtcagc caaacaaaag
    10201 ttacggttga atttggtaaa gaacgattca tttttcggca aaatcgtgtc gtccaagcag
    10261 gatggaaagg cgaaactgaa actgagacgg aaacagtcaa atgggaaaaa ggaatgagaa
    10321 tcaatcccga tttcgccatc aaaaaagaac tatctgcacc gcctaaacca ttgtcagaag
    10381 caagcttgct tggtcaaatg gaaaaataca gtctgggaac cccagctacg cgtgcagaga
    10441 ttatcgagaa actaatcaaa tctgaactga tggaacgtac ccctcaagga ctgcaagtat
    10501 ctcctaaagg taagcaatta ttagtattgg taaatccctc tttagtaaca ccagatttga
    10561 cagaaagctg ggaaaaagac cttgaagcta ttgctgcagg aaagaaacaa gcaggaacct
    10621 tcttgaatgg aatcgaaaaa gaaacaaaac gcttggtgaa tgagatcaaa tcaagcaaac
    10681 aagaatatca agatttctcg attacccaaa agaaatgccc agaatgtggg gccaatctgc
    10741 gcgagaaaaa cacacgtgac ggcaaaatct atgtctgcac caattcagat tgcaactacc
    10801 gcagaagaaa agatccaaaa gtctctaatc atcgatgccc gcaatgtcac cggaaaatgg
    10861 aaatcatcga aggtaaaaac ggtgcttatt tccgttgtaa atacgatgga acgacagaaa
    10921 aattgcttga taaaaaagag cagaagaaga aaatgaccaa acatgaagaa cgacgcttgc
    10981 tgaaaaaata tgcacaagca gaagaaccag aagaaagccc attagcagca gcactaaaag
    11041 cagcaatgaa gaagtaataa gatatgaaag aggttgtgat agaagtggtc agcttcaaga
    11101 aataagaagg aatttacgaa aattgcttct caaatttttg tgaaatttcg gcttatttcc
    11161 gaagaagcta cttctggaac accatttatt cggaaaaaag gactgtgaca agacttttgt
    11221 cacagtcctt tttcatttaa aaaaatgttt acttcatttg acgtatatta ttggtgaggc
    11281 ttcaattcat ttggcttcac tttttctgtt tctcttatga tataaacagg acgctgtttc
    11341 gtttctaaga agattttccc aatatatttc ccgatgattc caagacataa caattggatt
    11401 ccaccaatga acaggaagat cgagaccatg gaaggccatc ctgatgtagg atcaccaaac
    11461 aacaatgccc gcaaaataat gaacaaaatt gcaataccag aaccgacaca agacaaagcg
    11521 ccgataaatg aagcaatcgt taacggtgca tccgagaagt tcacgatacc atcaatcgag
    11581 tagttgaata atttccagaa ggaccaagat gtttcgccag ccacacgttc gcggttttca
    11641 taagaaatat attctgtttt aaatccgacc cagctaaata atccttttga aaatcggttg
    11701 tattctgtta attctaggac ggcatccacc atttgacgag tcatcaagcg gaaatcacga
    11761 gcgccatcga ccatttctgt atcgctgatt ttattgatca actggtagaa ttttttcgca
    11821 aagaaactgc gtatcgcagg ctcgccttca cgagtcgaac gccgggtgcc gacacaatcg
    11881 atatcagaag tagtgatccg gttgatcatc tcaggaagca gttcgggcgg atcttgcaga
    11941 tcaacatcca tcacggtcac taaatctcct gtagaagctt gcaatccagc gtacagagcc
    12001 gcttctttcc caaaatttct ggaaaatgag acaaagcgga cataaggata agctgaggct
    12061 agcttcttga ttgctgctaa cgtaccgtct tttgatccgt cgtcaataaa caaatattcg
    12121 aactcatggg cacctttgaa tttttcaacc tcatcaaaaa aaagaggaac agttttttct
    12181 tcattaaaac atgggacaac aattgatact ttcatcgttt ctatcctttc aatcgtatgt
    12241 ttatatatgg tttacattca aaatttaata aaccttaaat aacaccttta aaatttgatt
    12301 ttacgtaaaa gaacaggtat tctttttgta aactaacgta tttagtttac catgtttcct
    12361 cggtttatca aacttttaag aacatttttt attagttggc aagtagtttt ggatagagaa
    12421 aaaggaggaa gttccagtct attgactagg agcaaacaaa atgaaaatac acacacctat
    12481 cggcttttta tggttagatg cagatgaatc cggattgact gctgtatctt ttcagccaat
    12541 ttcagaaagc gaatcagcga atcagcgaat tttaacgcgc acacaagaac agtttgaaga
    12601 atattttgcg ggaaaaagaa aagaatttga tgttccttta tctataaaga gaggtacgcc
    12661 ttttcagcaa aatgtctgga aagctttgtc gaccattcct tatggagaaa tccgaacgta
    12721 taaagaagta gccgttgctg ttgatagtcc gaaagctgtg cgagcgattg ggcaggcaaa
    12781 tcgaaccaat ccattgccaa tcgttattcc ttgtcatcga gtgatcgggc aaaatggaaa
    12841 actgacagga tatatgggaa acgcaataga tggggttgaa atcaaacgcc aattgttgtt
    12901 gttagaagga tatctgcaaa attaaaaaca gttgtgattc ggacattgtt ccatgcgaga
    12961 atcacaactg ttttttcatg ttcattctaa actttgatca gccaaataaa tagtaaagcc
    13021 atcactgttt ggagaagccc gacagctaat ccataagata agaagcgtgt accttctttc
    13081 atgaattttg caaagtctaa acgcagacca attgccgcta aagcagtgat ctcaaaccag
    13141 ctgctgataa aatgtgccga ttggtcaata actacaggta aagaaaacaa gctgttgacc
    13201 aaacaaaaga tcacaaaccc taatacatac caaggaagcg gtaattttcg ttttttctgc
    13261 gtatcagttg gcgtttcaaa cagagctggt tgtttcttgt gcatgaattt ttcaaacaga
    13321 tagacaacaa caacgagcat cataatacgt agaatcttaa atagcatggc gaattcgaca
    13381 acagaattgt caagtaagct tgccccagcg actacttgac caacagattg gagtgtacca
    13441 cccaaaagtg cacttttttc aatgagattt ccaccaaaca actgtaatcc aagaaaaggg
    13501 agagtcaaca tcatcacagt acccagtaga ttgactagtg tgatgatctg tcctttttct
    13561 tcctctttcg catggatgga aggggcgatt gaagcaatag cagaagaacc acaaacagcg
    13621 tttcctccag ccatcatcaa agaagaagta tcgctgaatt gcagtttttt accaatcaag
    13681 taagcaccga tgatagtgac tgacatttgc agtaacacaa aaacaattcc ttttacacca
    13741 atttgagcaa tcgtttgagc tgtgacagtt gcacctagta aaaaaacaga gaattctaat
    13801 agtttgcttt cagcaaattt tgtgccgatt gctagaacag gctgtttaaa aaaagtattt
    13861 cctaataaaa tacccaataa aatagcaata gtcgctccac ctaactgtgg cagataaata
    13921 gcaagaagcc tgctgaaggc agcgatggca aaagaaagca gtaaaccggg taagatttcg
    13981 cggaccgctg aatcctttac agattttatt ggatgtgaca atgatttcat ttgatcggtt
    14041 cctttcgtca ataaatacta aaagcatact cgaacatagt agcactaaac cagtattttg
    14101 acaaaagaac aagaggtcgt gacaaaagtc ttgtacgacc tcttgtttcg aataaacggt
    14161 gttccagaag tagcttctac ggaaataagc cgaaatttca caaaaatttg agaagcaatt
    14221 ttcgtaaatt ccttcttatt tcttgaagct gaccacttct tttacgacct cttggtaaac
    14281 ggtgttcaaa agcttgcatc tgcggtaata agcccaacca gtacaaaaac atgaacagct
    14341 gtttttgttt ggccgttctt attacttgat gcagaacagc tttttcacaa cctattgaat
    14401 cgattattaa gcgatttttt tagaaacaaa ttttccatgt ttgctatgtt ctttaattaa
    14461 gaaacgtacg taatgagcaa gaacgtagaa gacaactaca cttgcaaaca gacattccgc
    14521 aacttttaag aaatccaata cgctactata gatacctaaa ctgttgaagt aatgaacagt
    14581 agtaaatata gctgttgcag agataacaag cgggaatgta aaggctgcat agcttggata
    14641 aaaacgaagc ttgaacaaac atggaagcat agacagcaca acgaaataca atacttgaga
    14701 taaagcgaac aatgctacga caagataaaa attaggttca gcaaagtttt taagataacc
    14761 tgttaggcaa agagaaccag gtgctgcaac gatcgtaatc agcggacgtg tcggatgagc
    14821 catatttctt acgaaaaaga cacgtttgat gatgatcggc aataagaacg catagcagat
    14881 caatgaaatc caaaagaaaa tttttccaat aagtgggaag aagttcccgg cagttacagt
    14941 gatgacccca aatccaacgt aaatgataaa ccagcttgga taaattgctt caattgaaag
    15001 atcagctttg ataacatggt gataattgaa ataaaagaca aggatcactt ggaagataac
    15061 agcgcacagc caaatgaatt tgacaattgg tgcaatactt tcatatgaaa caaaataagt
    15121 acaaataacc atgagtgaca tcgtaaaagt tggtgaaacg gaggcgatga ttggatcatg
    15181 caacgtttgt ttcgtgtgtt cgaataagat aaacaattta acaaaaatga atatcataag
    15241 aatcatagag ataccgccag ttagattgcc taaagcaaca tggtgatagt ctttaaacag
    15301 atttccaagg gaagctaacc caaggatcaa cccgcagatc gggatcggaa taattttcaa
    15361 aaagttcttc atatataata ttctcctttt tatttaatag attttagtac caagatgaac
    15421 gaatacattg taaaatgaat aggaatgtat gattacgagc aagcagacag ctgattgtct
    15481 tcatctaagt tactagttgg atcagcaact gttttgttgt acatttctat tatagatggt
    15541 tttttgtata tttaaatcaa atagttttta tagtaattat cataaaaagt tatggaagtg
    15601 gtgccatgtt taagttatta acgacttttc gagcagtata tgaaacaaga aatttttcca
    15661 gagcagcaga agtactgttt ttatctcaac cagcagtctc gaatcaaatc aaacagttgg
    15721 agcgggaatt gaatatcgat ctttttcaac gtaacggaag aaaagagatg cttccaacta
    15781 agcaagccga cttattatat gaacggacac tggttttgtt agaagaatgg gaagaaactc
    15841 agcaaaaatt gctcaatgaa caaaatgaag aagaagtatg tcggatcgtt gcttcccaca
    15901 cctttgctgt ctatcttttg cctcagttga tgaaacatct gatcaaaagg tttcctaaag
    15961 tgcattttga gatcgaaatc acgaattctc atgaatcatt agaacaagta gcgaaacatg
    16021 aatccgattt tggttttatc gaaaagccat tagagacttc cggcgtacaa agatactctt
    16081 tgatggaaga ccagctagtc atcgctgggg acccaaaaag caaattgtgg cttgtaagag
    16141 aagcaaactc tggtgttttt cattacacgc agcgttattt tgaagagatg aatatccaag
    16201 ataagaagat gatcgtgaaa agtaacgatg taattgttgc gttgctgaag gaaggaatcg
    16261 gtcgctcgat tttgtctaag cgctctgttc cagaaaatgt gccaatgact ccgttgaatt
    16321 tccatcgata tttttatttc atccaacgaa ccaatctggt ttctgatcag ctgctagaga
    16381 ttgccgaaga gatccttcga tacaatgaaa gcacagaaac ttctcacagc gaggagaatc
    16441 tcagctggta gataattgca tgtatcaaaa aaagttctat gatacactaa agataagcag
    16501 taggcttttg tactgcattt gctcaagcaa ctgcacaaac ggaaagttaa tttttcccaa
    16561 atgtctatgg aaagactttc cattatctaa ctggaccgct ataataatca gcagtatggt
    16621 ttttaagaaa agggatgaaa catggaaaaa aaattaaaag cttcgaacga taatcgtatt
    16681 gattatggcg tgattttacc agtctttctg ttatgtttga ttggtctgct ctcattatat
    16741 gtagcactta gccatgatcc gaacaatcca gcagtcttga aaggcgtagg gatgcaagtt
    16801 gtctggtatt tagttggagc ggcagcaatc gttgtcatca tgcatttgaa ttccaaatgg
    16861 atctggaaac tgactcctta cctttatggt ttggggttag tggtaatggg actcttgctc
    16921 aaattctatg atcagaattt agctgcttca acagggtcta aaaactggtt tcgattcggt
    16981 gcgtttactt tccagccagc agagttgatg aagattgcgt acatcttgat gatggcactg
    17041 atcgtaacta aacataatac gcaagtaaaa gaacggacga tgaaatcgga cttttggctg
    17101 attgggaagc tcttgatcgt gacaataccg gttttagcat tgatcatggc ccaagatgac
    17161 tttggaacga tgcttgtctt tcttgcgatc tttggcggga ttttcctgat gtctggaatc
    17221 tcttggcgca tcatcgcacc tgtagtaatc ttggcagtag tcgttggagc aggaacgatc
    17281 ttcttggtta cgactgaagg cggaagagac ttgctttata aagttggatt caaatcttat
    17341 cagtttgcac gtattgattc atggctggac cctttccatg atacgtcagg aatgagttat
    17401 cagccggccc aaggactttt ggcaattggt accggtggct tgtttggtaa aggattcaac
    17461 gtaagcaata tctatgtgcc tgttcgtgag tccgatatga tttttaccgt tatcggtgaa
    17521 aacttcggct ttatcggtgg tgcattcgtc attttcttgt attttatttt aatctatcgt
    17581 atgattcggg tgtgtttcga tacgaataat gaattttacg cctatatcgc aagcggtttg
    17641 atcatgatgc tgctgttcca tgtctttgag aatattggag caaatatcgg attacttcct
    17701 ttaacaggga tcccattgcc tttcattagt cagggaggat cctcgatttt agggaacatg
    17761 atcggtatcg ggttgatatt atcgatgaga taccaaaatg aagcacctgc aagatcttca
    17821 aggaggtaag aaaattgtac accttttact ggtacccgaa atgttcaaca tgcaaaaaag
    17881 ccaaagcgtg gttagaccag catggcgtta gttatcaaac cgttgatatg atcaaagaag
    17941 taccgaaggc ctcaacgatt gagacttggc tagaaacgag tcaagtacca atccgtcgcc
    18001 tgttcaacac aagcgggatg aaatatcggg aacttggact gaaagatcaa gtaggcgagt
    18061 ttacggcttc aaaagcgagc caagtcttat cgacagatgg tatgctgatc aagcgaccac
    18121 tgatcttacg agaggaccag cttgttgcaa ttggattcaa tgaaaaaatt tatgaaggag
    18181 aactgatata aaatggcagg caagcatttg aagaaaaagg acaacctttg gatcttattt
    18241 aatggaaaag aatactgtgt tggtttgaca aatgaagcgc aagaagaact tggagacatc
    18301 acgtttgcca atctgccaaa agcaggacaa tcgctaaaaa aagatgaacc tctgatcgaa
    18361 gttgaagcgg aaaaagcggt caatgaattt gccagcccgc taacgggtac agtttcttct
    18421 gttaatgata aaatcggtga agatatcaat atcttgaatg attcagatga aatgaatgcg
    18481 tggatcttaa gtttcaaaga tgttgatcca aaagaatttg acgaattata aaatcagaaa
    18541 atcacttgaa aaaagttgac actgattctt ccagttgcta taatcataag caatcctata
    18601 tgaaacgaga aagctttgaa aagaagagta catgtcacgg acgtttgaag agagccctgt
    18661 ttgctgaaaa tgggtaacga aaatgagatg gaagatggtc ttagagcaga gtaagcgagc
    18721 gtaagcaaac ttactacggg agtgcccgtt acagcgcgac agtatacgga aagaagctaa
    18781 gtccaagtac tggaagaggc tgttattgcg aagtaacggt aaatcaaggt ggtaacacgg
    18841 aagttcaagc tttcgtcctt ttgcttaagg caagagggcg aaggcttttt tgtttgaaaa
    18901 atcataagag aaagatgtga atggcatgtc attaatccaa ctaacgaatg tcaaaaagac
    18961 attcaatggg aaaaacggaa acatccaagc cgtcaatgac gtttcactaa acgttgaaaa
    19021 aggggatatt tatggaatcg tcggctattc tggcgcagga aaaagtacat tagtccgtct
    19081 cctgaatgga cttgagcttc ccacgagcgg cgaagtgatc gtaaacgatc aagatattac
    19141 aaaattaaaa aacaaagaat tacgtacatt ccgaaagaaa atcggtatga tcttccagca
    19201 tttcaacctg ttatggtcaa gaactgtact tgaaaatatc cagctgccgc tggaattagc
    19261 tggcgtacca aagagcaaac gtaaagaacg ggcagaagaa ctgttgcgtt tagtcgggtt
    19321 agaaggacgc ggacaagcat atccttcaca actttccggc ggacagaaac aacgcgtcgg
    19381 tatcgcacga gcattggcaa atgatcctga gatcctgctt tgtgatgagg ctactagcgc
    19441 attggacccg caaacaacag aagaagtatt agacttattg ttagcgatca ataagaaatt
    19501 gaatttgacc atcgtcttga tcacacatga aatgaatgtg atccgcaaaa tctgcaacaa
    19561 agtcgctgtg atggagttgg ggaaagtcgt agaagaagga gacgtattga ctgtcttcag
    19621 acaaccgaaa aaagaagtaa ccaaacgctt tgtacaacaa gacatcgaac cagaagctga
    19681 ttcgacagaa ctattagctg aactcatcaa agaaaatcca agcggccgtt tggcaacttt
    19741 gacctttagc gaaagaaatg ccaacgagcc agtcatttcc caagcgattc gacgctataa
    19801 catcgatgtc aacgttgtgt atggaaagat caaacaagtg aaagaaggtt cctttggttc
    19861 attgactgtc ttgatgactg gtgaaaccaa agaattagat caagcagaag tgttcatcaa
    19921 agaacaagga gtaggaattg aggtgatcca tcgtggataa aagcttttca gaaacatatc
    19981 tagattttaa acaaatcaac ccggaaacga tgaatcaggc tatccttgat acactatata
    20041 tgacattaat ctcaatggtc ttagtgacga tcatcggatt agttttggga ttgatccttt
    20101 actctattgg acgcaaaaac aaaccgtatg ctcgaatcat ttatggaatc gtatcgatca
    20161 tcagtaatat tttccggtca acgccattta tgatcctgat ggttttgatc attccattta
    20221 ctaaagcaat cgtaggcact atgctaggtg cgcaagcagc cattccagct cttgttttat
    20281 ccgcagcacc attttatgcc cgtctagtag agattgcttt tcgtgaagtc agccctggtg
    20341 tactggaagc agcagatgca atgggagcaa gttactggga aatcatctgg aaggtcttga
    20401 tacctgaaag tattcccgca ttgatttctg gattaacagt tacaacgatc tcgatgatcg
    20461 gatttactgc gatggccggc gcgatcggcg caggtggact tggaggtctt gcttggcagg
    20521 aaggctacca gcgtggtaat ctaacagtga cattcgttgc gactttaatt attttactaa
    20581 tcgtatttat cgttcaagga atcggtgatt tcttaactaa gaaaacagac aaaagataat
    20641 aggatatagg gggatgtagg atatgaaaaa gaaaattttt ggatttgcag cagcgctttt
    20701 gctgactgta ggattagcag catgcggtaa caacagcgac agcaaagatt cagcaaataa
    20761 agcagacgac acgacattga aagtcggcgc ttcaccgaca ccgcacgcag agattttgga
    20821 acatgtgaaa ccattattga aagatgaagg aatcgatctt gaaatcgtca aattcgatga
    20881 ctatgtatta ccgaaccaat ctttggaaga aggcgacatt gatgccaatt acttccagca
    20941 tattccttat ttgaacaaag aaatcaaaga aaaaggatat gactttgtca atgccggtgc
    21001 tgtccacatc gaaccaatgg gcttgtactc taaaaaagtc aaagatgttt ccgaattaaa
    21061 agatggtgca acaatcatta cttcaaattc tgaatcagac tggggacgca tcatcacaat
    21121 tctacaagat gctggcttag tcaaagtcaa agatggagta gatctggaaa cagcaacatt
    21181 cgatgatatt gaagaaaacc ctaagaactt gaagttcaac catacgatcg acccatcact
    21241 tttagcttct acgtaccaaa atgatgaagg tgacctagtt gcaatcaacg caaacttcgc
    21301 ttacggcgca gggttgaatc cattagaaga tgccgtattg cttgaaaagg acaactctcc
    21361 ttacgtgaat attattgcga ctcgtaaagg tgatgaagac agtgacaaaa tcaaaaaact
    21421 gattgaagtg cttcaccgca aagaagtcca agaatggatc acagacaaat ggggcggctc
    21481 tgttaaacca gtaaaggctg atgcaaaata aggtatggaa caaaaaagat gagaaataag
    21541 tctgtaatgg cttatttctc atctttttta tacctattct aatttagaaa tcaaaattgt
    21601 ttaataataa ataaaagata tttgatttta gtaaggaagg tcattgcgct tcattctcaa
    21661 ttagtctaaa atgagtgaga acgaaagaga atttgggggt ataatgcatg tctgtcttag
    21721 aaataaaaaa tctacatgtg tcgatcgagg aaaaagaaat ccttaaaggc gtaaacctaa
    21781 caatgaaaac cggagaaatc catgccatca tgggacctaa cggcacagga aaatctacct
    21841 tatctgccgc tatcatgggc aatccaaact atgaagtaac agaaggcgaa attttgtttg
    21901 acgggaaaaa tgttttagac ttggaagtag atgaacgtgc gcgacttggc ttatttttag
    21961 ctatgcaata tccaagtgag ataccaggta tcacgaatgc ggaattcatg cgtgcagcga
    22021 tcaatgcgaa acgtgatgaa aacgacaaaa tttctgttat ggcatttttg aaaaagttag
    22081 atgaaaaaat ggctttacta aatatgccag aagaaatggc tgaacgttat ctgaacgaag
    22141 gtttttctgg cggagaaaag aaacggaatg agatcctgca attgttgatg ttagaaccta
    22201 catttgcaat tttagacgaa atcgattcag gtctggatat cgatgctttg aaagtcgttg
    22261 ctaaaggagt aaatgaaatg cgcggagaca atttcggtgc attgatcatc acccactatc
    22321 aacgattatt gaactatatc acacctgatg tcgttcacat catgatggaa ggccgagtag
    22381 tcatgacagg gaatgctgac ctagctaaac gtctggaagc agaaggctac gctggtatca
    22441 gtaaagaatt aggaatcgac tataaagaag aagaagcgta agggggagca agaatgaaag
    22501 aaaaaaactg gctcgattat cttgacgcgg taaatgattt ttctttaagt aaaggcgaac
    22561 ctgattggat gagaaccttc cgtcaagacg cactagcgaa agcagatgaa ctgccattgc
    22621 cgcatatcga tcgggtgaaa ttccatcgct ggtcgttatt tgatgtgaaa gaaacacaaa
    22681 ccatcagtga aacaggcacg attccggcat ttgatgcaat gaaagacaat cctgtgcttg
    22741 tccaacaagg ttcatggacg atttttgaac aactgcctgt ggaattagca gaaaaaggag
    22801 tcatttttac cgacttgttc actgcaatga tcgaatatcc agaactagtc caagaatatt
    22861 atatgaaaaa agcagtaaac atgaacgaag accaattgac tgcactccat gtagctttta
    22921 tgaacagcgg tattttcctg tatgtgccta aaaatgtcgt gatcgatgaa ccactcgagt
    22981 ccttattcat tcaagatggc gcatccgatg agcacttctt caagcatgtg ttgatcgtgg
    23041 cagatgaaca tagtgaattt tcttatctgg aacgtttcca gacaaccaaa gaacaagtag
    23101 caaaatcatc aggtaatatc atcgtggaag tcatcgctaa agctggttca aaaatcaaat
    23161 attcagccgt agatcaatta ggagaaaata ttacttctta tatgaatcgc cgtgggcaca
    23221 ttttacgaga tgcttctgtc gactgggcaa ttggtgtcat gaatgacggt catgtgatcg
    23281 ctgactttga ctcagattta gcaggagaag gtgcacacgc agaagtcaaa atcgtggcaa
    23341 tcagctcggg cagacaaatc caaggaatcg atacacgagt aaccaataaa gcccctcata
    23401 cgatcgggca tattttgcaa cacggagtga tccgagaaaa aggaacattg acctttaatg
    23461 gtatcggcca catcttgaaa ggggcaaaag gtgcagatgc ccaacaagaa agtcgtgtct
    23521 tgatgctttc tgataaagca cgtggtgatg cgaatcctat tcttttgatc gatgaaaacg
    23581 aagtaactgc tggacatgct gccagtgtag gacgagttga tccagaagaa atgtactatc
    23641 tgatgagtag aggattgcat aaagaagaag cagagcgctt agtgattcga ggattcctcg
    23701 gttctgtctt aacagcaatc cctgtagaac aagttcggaa agaattagtc gaagtaatcg
    23761 aagggaagtt gaatggatga tcgatgctaa aaagctacgc aatgattttc ctatcttatt
    23821 tcaagacgtc aatgatgaac ctttagttta tctggataat gcagcgacga cccaaaagcc
    23881 aaaacaagta ctccaggcat tagcgtacta ttacgaacac gataacgcca atgttcatcg
    23941 cggtgtccat acgttagcag aacgcgcgac ccatgattat gaacaagcaa gagaaaaagt
    24001 acgggcgttc atccatgcaa aagaaacagc agaagtgctg tttactcgtg gtacgacgac
    24061 aagcttgaat tgggtagcca gaaccttcgg cgaccgctat atcaaagcag gagacgagat
    24121 agtgctctct tacatggaac atcattctaa tgttatcccg tggcagcagt tggcaaaacg
    24181 aacaggtgct gtcttgaaat atatcgaact aacagaagaa ggcttcctgg atatggagga
    24241 tgcagcgaaa aagattacgg acaaaaccaa aatcgtctcc attgctcatg tatccaatgt
    24301 cttaggtgtc ttcaatcctg ttcaagaatt agctgaactt gtccatgaaa acggcggggt
    24361 catcgtagta gatggtgctc aatctgcccc gcacatggcc attgatgtcc aagcactaga
    24421 ctgtgatttc tttgctttca gcggacacaa aatgtgcgga ccaacaggta ttggtgtttt
    24481 atatggtaaa cgaaaatggt tagaagaaat ggaacctgtt gaatttggtg gcgaaatgat
    24541 cgattttgtc gaattatacg acagtacatg gaaagaacta ccttggaaat tcgaagcagg
    24601 gacaccaaat atcgccggcg ctatcgcatt aggccatgca atcgattatt tgcaaaaact
    24661 cggtatggac aatatccatc gatatgaaga agaactagcc gcttatgtgt tgccaaaatt
    24721 acaagcaatt gatggattga ctgtttatgg tccacaagac ccgaattttc ggacaggagt
    24781 cgttgcattc aatttggatg ggttacatcc ccatgatgtc gcgacagctt tagacatgga
    24841 aggggtagcc gtcagagcag gtcatcattg tgcgcaaccg ctattgaagt accttgaagt
    24901 atcagcgact gctcgcgcga gcttttattt ctacaacaca acgcaagacg ccgaccgttt
    24961 agttgaagca atcatggcaa caaaggagtt tttccaacat gggactatct aaattagaca
    25021 atttataccg tcaagtgatc ttggatcatt cttcccatcc gcatcatcgt ggaagcttga
    25081 ctgactcgac aagcaagatc gaattgaaca accccacatg tggagatgtg atcgaattgc
    25141 aagtcgatgt aaaagacgga atcatcaaaa atatcgcctt tgacggcagt ggctgctcca
    25201 tcagtacggc aagtgcctcg atgatgacag acgccgtcat cggaaaatca atagaagaag
    25261 cagaacattt aactgtagac tttctacagc ttgtccaagg aaaagaagtc aaagatcccg
    25321 accaattagg cgatgctgct atgcttggtg gtgtagctaa atttcccgct cggatcaaat
    25381 gtgcgacact tgcctggaaa gcactaggaa aagcaatgga aaacgatggt accgcagctt
    25441 tccatcatta ccacaaggaa gaggagtgag aacatgggcg taccagaatt agaagaatat
    25501 aaattcgggt ttcacgatga tgtcgaaccg gttttcagta caggagaagg cttgacagaa
    25561 gaagtgatcc gtgagatgtc aagaatcaaa ggcgaaccag aatggatgct tgaattccgt
    25621 ctgaaatctc tagaaacatt taacaaaatg ccaatgcaaa catggggccc cgacctttca
    25681 gacatcgatt tcgatgccat caaatattac caaaaaccaa gtgacaaacc cgctcgtgac
    25741 tgggaagacg taccagaaaa aatcaaagat acatttgaaa agattggtat accagaagct
    25801 gaacgagctt atctagccgg tgcttccgca caatatgaat cagaagtcgt gtaccataat
    25861 atgaaagatg aattcgaaaa gttaggaatc gtctttactg acactgactc tgctttgaaa
    25921 gaataccctg aattattcaa agagtacttt tcaaaacttg taccgccaac cgacaacaaa
    25981 ttagcagcgc tgaattcagc cgtatggtca ggtgggacct ttatttacgt gccaaaaggc
    26041 gtcaaagtcg atgttccatt acaaacgtac ttccggatca atgcggaaaa cacggggcaa
    26101 ttcgaacgta cgctgatcat tgttgacgaa ggagcaagca tccactatgt cgaaggttgt
    26161 acggcaccta cttattcaag taacagcctg catgccgcca tcgtagaaat ctttacaaga
    26221 aaagatgcgt attgccgtta cacaacgatc caaaactggt cagacaatgt gtacaactta
    26281 gtaaccaaac gagcaaaagc atacgaaggc gcaaccgttg aatggatcga cggaaaccta
    26341 ggtgcaaaaa caacgatgaa atacccaagt gtttacttag atggaaaagg tgcacgaggc
    26401 accatgctct ccatcgcctt tgcaggagca aaccaaatcc aagatacggg tgcaaaaatg
    26461 atccacaatg caccaaatac atctagctct atcgtctcta aatcaatttc aaaagacggc
    26521 ggagaagtaa actatcgagg acaagtaacc tttgccaaaa acagtgcggg ttcgatctct
    26581 catatcgaat gtgacacgat catcatggac gaatggtcga aatccgatac gatcccattc
    26641 aatgagatcc acaacagcca agtctcttta gaacacgaag ccaaagtctc aaaaatctca
    26701 gaagaacaac tctactatct gatgagccga ggcctgaccg aatctgaagc aactgaaatg
    26761 atcgtcatgg gctttgttga accattcacc aaagaacttc caatggaata tgcagtcgaa
    26821 ctcaatcgat tgatcagtta tgagatggaa ggcagtgttg ggtaaacctg ttataggagc
    26881 tagaaccctt gatatataag ggttctagct tttttgtttt gcctgtatac tagaaatgac
    26941 cagattttga ccagctcttt tactgatgag ccatcatatc agcaaagtct tgacctgttt
    27001 tcttaggtgc attatttgta atatgtgcat agatgtctaa tgtcacttta ctattttcat
    27061 gccctaatcg ttgttgaact accttgacag attctcctgc ttctaataat aaactagcat
    27121 gtgtatgtct aaacccatga ggtgtaatac gaggcaagtt atattttttc aaaatatatc
    27181 ttaaccaatc attaacagtg tttggaatgt atagagtgtt tttaactgtt gtaaaaacat
    27241 gttgctcttt agagctagta ttgtatccaa gttttaaata ctcttctttt tgctgtaatt
    27301 tccagttgtt gaggatagaa agagtctcag tatctaaaga gataactcgt ctagaagctc
    27361 ttgttttcgg agattgaatt ataactttac caaattcatc tacagcaaga gttttattga
    27421 catgtagttc tttattaaac gtgtctatgt ctttccactg taaagcgagt acttcactct
    27481 ttctcatacc tgtgtatgcc aataatctga agaaggcgta ttgtttcatg ttaccaaaat
    27541 ctttaaaagc atccagtaaa ttattaagct cctcttttga gtaaaattta cgatttttct
    27601 catactcttt ttttcgtgga agaagtgttt tttccatagg attattgtca atatattgca
    27661 ttgcaacccc gtagcgtaaa atttgtgcag tctgttttct catataatcg taactttcat
    27721 atttactgtg ccattcatct accacttttt gacaatatgg gacagagata ttcgatagtt
    27781 taagcttacc aaatgctgga agtatttgat tatttgcgta tcttactgag attgcgattg
    27841 ttgaagcttt aacactggta cgctgatgag ttatccattc attatacagg tcttcaaatg
    27901 tcatatcagg tgctttcttt ttaacaccgt ataatatttg atgtctgtac tcgtcataat
    27961 ctttttgagc ttcttgtttg ctactaaaac ctcttctcgt agtatttatt ttttcaccag
    28021 ttcgtggatc ggtggcaatg tgtcctttat acatccacgc cacttgaccg tttttctttt
    28081 tgtactttga tatttttgcc attaaatcat ctcctcttag tttacaagga gtacttacca
    28141 ctagatggta tcacctccct tcattttttt cttagtgtat tttttcccaa ttgatgaaat
    28201 cgtttgttct agcttgttta tattatcagt aatttctctc acagcttctt cgggataaga
    28261 catgtctttc aattgaatag gtagccattc atctaccatt ctttctagta gttcttctat
    28321 actgctagtg aggaaaagaa tgttattgtc aaattcttct acaatagcat tgatgaataa
    28381 ttcttctatt tttaagatat tgaaaagact aattttttca tttttaactt tattacaagt
    28441 cttgttagca ataaattctt gcatctctgg ttctaatttg gaatatctat ccagccatgt
    28501 atctaaatta gagtgtactg gtgggaactg ttgacgaaga tgctggaaca ggtttttatc
    28561 tggaaatgtt ataccatcta gtacaatgct atctctataa agaataattc ccaaaatata
    28621 ttcttctaca ttgccacaaa ctaactcctc tatactcata tttcccaaat cagcaattcg
    28681 ggataatcgc tctggtattg gtaaataggt tcccttctcc cattcgtaga tagttttttt
    28741 gtcaattaat tgaccgttgt taatcataag accaaatgct ggttgtgtta attttttact
    28801 ctttcttata gcttttattt ttgttccgag tatctttttt cgttctaaat aatttattgg
    28861 tggacgtttt atcatattga ccacacctcc taaaattagt atacctcaaa aaaggggtgt
    28921 tgcaaataaa ttttacaaag ctgttgacac ccctttttta tcgaggtatt atgtgtatgt
    28981 cgatgaactg gtaatcattt tttgtttgat taatcggtta ataacaaaat acttttattt
    29041 tgagcaaaaa atacgaagat aattcttaga aaatttgttc aagcaatcac gaaaggaggg
    29101 ggaaatatgt ctctacaaat cattgatagc attgtttcta tacaagttga acaacaagtg
    29161 aaagagataa ttacagtgct aaagcctatg ctagaagaca ttatttctgg agtcactcta
    29221 gagtatatgg actatcagca ggcaagtgaa tatctaggtg tatcagtagg aacgattaga
    29281 aaatatgttt ctcaatacgg gttacctgtt ataaaaattg atacggtcat tagatttaaa
    29341 aaatctgata ttgatagttt catggaacgg tataaaggtt gagtaaacaa ataagactaa
    29401 aacatatagt aataaagagt ttgtttttat tatctgtatg ttttagtctt taacagaatt
    29461 ttataaggag gaaacacaaa tggaaacatc aatgattaat attccaattt atgtagatca
    29521 agaaaaatta ttagagaagc cagttacaaa aaaagaaaaa actgatgtaa gagataggtg
    29581 tttagataac tatagagaag ttacaagaga tgagtttgta gagatactaa attcatcaca
    29641 atcatttatt ccaagcaaga caaagaataa aggcaatgac aaggagagtt ttgtggaaac
    29701 cagagtaatt attctggatg tcgataacac tgttaaagat gagaagggga aggtagtaga
    29761 tttgagtaaa gatgatagta gatacttaag cgtagaaaag gttttatcta tcaatttagt
    29821 tcataattct gcgtttgcga tacaaaaaag tattcggtac tctgaaaatc tagaaaaata
    29881 caagattgtc tttctattaa aagaagcaat tactgactat ggtaaaatgg ttgctgttta
    29941 cgaatacttg aaaaagaaca tccccgggtg tgatgacaat gtaaatgctt cgaatagaat
    30001 gttttttgga ggatataagt cagatgcagt gataataatt aatgaagaca acatgttaga
    30061 tattactgag cttccgattg attttaccat gacatgttct aaaaattgtc aaagtgattc
    30121 agggagagag tatgaactag tcaatgaaac cgattttgtt aagttgataa aaaatgacga
    30181 caaagaagaa atgaaacagt ggtttaaaga ctgcctgttt gattcctccg acatgtcttt
    30241 taatgagatt tatgaaagat tgttaaccat tgatatgaat aaattactta agtcaagtaa
    30301 aaacttaaga tgtctgtttc acaacgatca taatccctct gctagtattt ttaccagtaa
    30361 aactgggcat tccatttttt attgtcattc cagtaattgt gatatcagtg aaaattttat
    30421 tggagttgtg atgctaattt taaagaaaga cagtcgggta gaaacattta attggctatt
    30481 agagaaccta gacttgtatc ctacacattt tcgaaaattg aaagaggaat caaaagtact
    30541 ttttgacact cttgaatcaa tgaaagacaa acttttcagg acaataagga attatctaga
    30601 ggaaatgcga cgtgtctatg atgtcttgct ttctgatgtt aatttttttg atgatagtaa
    30661 ggaaagtgtg acttgtattt tgtcggggga acaattagct aaaaaaatga gttctta
//
LOCUS       DACDMM010000018        29868 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDMM010000018 DACDMM010000000
VERSION     DACDMM010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.018"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(88..1137)
                     /gene="recA"
                     /locus_tag="IWD16_000616"
     CDS             complement(88..1137)
                     /gene="recA"
                     /locus_tag="IWD16_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP9087700.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1246..2487)
                     /locus_tag="IWD16_000617"
     CDS             complement(1246..2487)
                     /locus_tag="IWD16_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP9087701.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2727..3305)
                     /gene="pgsA"
                     /locus_tag="IWD16_000618"
     CDS             complement(2727..3305)
                     /gene="pgsA"
                     /locus_tag="IWD16_000618"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP9087702.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3514..4398)
                     /locus_tag="IWD16_000619"
     CDS             complement(3514..4398)
                     /locus_tag="IWD16_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9087703.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(5176..6462)
                     /locus_tag="IWD16_000620"
     CDS             complement(5176..6462)
                     /locus_tag="IWD16_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9087704.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6455..7723)
                     /locus_tag="IWD16_000621"
     CDS             complement(6455..7723)
                     /locus_tag="IWD16_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9087705.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            7975..8436
                     /gene="mscL"
                     /locus_tag="IWD16_000622"
     CDS             7975..8436
                     /gene="mscL"
                     /locus_tag="IWD16_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP9087706.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8496..8942)
                     /locus_tag="IWD16_000623"
     CDS             complement(8496..8942)
                     /locus_tag="IWD16_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9087707.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9035..9280)
                     /locus_tag="IWD16_000624"
     CDS             complement(9035..9280)
                     /locus_tag="IWD16_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087708.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(9396..9974)
                     /locus_tag="IWD16_000625"
     CDS             complement(9396..9974)
                     /locus_tag="IWD16_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9087709.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(10051..10581)
                     /locus_tag="IWD16_000626"
     CDS             complement(10051..10581)
                     /locus_tag="IWD16_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP9087710.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(10585..11445)
                     /gene="rlmB"
                     /locus_tag="IWD16_000627"
     CDS             complement(10585..11445)
                     /gene="rlmB"
                     /locus_tag="IWD16_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP9087711.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(11438..11833)
                     /locus_tag="IWD16_000628"
     CDS             complement(11438..11833)
                     /locus_tag="IWD16_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9087712.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(11830..13242)
                     /locus_tag="IWD16_000629"
     CDS             complement(11830..13242)
                     /locus_tag="IWD16_000629"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP9087713.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(13239..13775)
                     /gene="cysE"
                     /locus_tag="IWD16_000630"
     CDS             complement(13239..13775)
                     /gene="cysE"
                     /locus_tag="IWD16_000630"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP9087714.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(14097..15557)
                     /locus_tag="IWD16_000631"
     CDS             complement(14097..15557)
                     /locus_tag="IWD16_000631"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP9087715.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(15727..16884)
                     /locus_tag="IWD16_000632"
     CDS             complement(15727..16884)
                     /locus_tag="IWD16_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP9087716.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(16918..18288)
                     /gene="radA"
                     /locus_tag="IWD16_000633"
     CDS             complement(16918..18288)
                     /gene="radA"
                     /locus_tag="IWD16_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP9087717.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(18351..18878)
                     /locus_tag="IWD16_000634"
     CDS             complement(18351..18878)
                     /locus_tag="IWD16_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP9087718.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            19108..19914
                     /gene="proB"
                     /locus_tag="IWD16_000635"
     CDS             19108..19914
                     /gene="proB"
                     /locus_tag="IWD16_000635"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP9087719.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            19911..21158
                     /locus_tag="IWD16_000636"
     CDS             19911..21158
                     /locus_tag="IWD16_000636"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP9087720.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            21234..21578
                     /locus_tag="IWD16_000637"
     CDS             21234..21578
                     /locus_tag="IWD16_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087721.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(21695..22021)
                     /locus_tag="IWD16_000638"
     CDS             complement(21695..22021)
                     /locus_tag="IWD16_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087722.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(22636..23838)
                     /locus_tag="IWD16_000639"
     CDS             complement(22636..23838)
                     /locus_tag="IWD16_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9087723.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            23955..24470
                     /locus_tag="IWD16_000640"
     CDS             23955..24470
                     /locus_tag="IWD16_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9087724.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(24669..25115)
                     /locus_tag="IWD16_000641"
     CDS             complement(24669..25115)
                     /locus_tag="IWD16_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9087725.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(25162..26640)
                     /gene="msr"
                     /locus_tag="IWD16_000642"
     CDS             complement(25162..26640)
                     /gene="msr"
                     /locus_tag="IWD16_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9087726.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(26994..27623)
                     /locus_tag="IWD16_000643"
     CDS             complement(26994..27623)
                     /locus_tag="IWD16_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9087727.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(27607..28071)
                     /locus_tag="IWD16_000644"
     CDS             complement(27607..28071)
                     /locus_tag="IWD16_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9087728.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(28558..29826)
                     /locus_tag="IWD16_000645"
     CDS             complement(28558..29826)
                     /locus_tag="IWD16_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9087729.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
BASE COUNT         8353 a         6421 c         5366 g         9728 t
ORIGIN      
        1 actcacgatt tctttattca ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag
       61 accagccgtt tttgtttatc ataattttta ttcttctact aaaggcaatt cttcttgtcc
      121 tttttcatct tctgggacag cgacttcttc accaatacca taagctgctc taactagagc
      181 agaaacttct gccatcattt caggatgatt agccatatag attttagcgt tttcccgtcc
      241 ttgaccaatc cgatcttctt tataagaata ccaagcaccg cttttatcaa caatgtcttt
      301 ttctactgcc atgtctagaa gttctccttc ttgtgagatc cctaaaccat acatgacatc
      361 tacttcagcg attttgaaag gaggtgctac tttgtttttt acgactttga tctttgtacg
      421 gttaccaaca atatctgtac cttgtttcaa ttgttctgca cgacgtactt ccaaacggat
      481 cgttgcgtag aatttcaatg cgcgtcctcc aggagtgatt tctggattcc caaacatcac
      541 gcctactttt tcacggattt ggttgatgaa aatagcgatt gtttttgttt tgttgatcga
      601 accagagagt ttacgcaatg cttgagacat caaacgtgct tgtaacccga catgtgagtc
      661 acccatttcg ccgtcgattt ctgctcgtgg aactaaagca gcaactgaat cgactactac
      721 gatatctaca gccccacttg atactaaagc atcagcgatc tctagacctt gttctcctgt
      781 gtcaggctgt gaaagaagta gttcatcgat attcacacct aatttttgtg catattgcgg
      841 atctaacgca tgctcagcat caatgaaagc ggccgttccg ccattttttt gtacttctgc
      901 aatagcgtgt agtgcaactg ttgttttacc tgaactttca ggtccatata cttcgatgat
      961 acgtccacgg gggtaaccgc cgacacctaa agcgacatct aacgcaagag agccacttgg
     1021 aattgtagag atttgttggt cgattttttc gcccaatttc ataatagagc ctttaccata
     1081 gctcttttca atttttttca gtgcagcatc taaggctgct ttacgatcat ctgccaatac
     1141 gataatcctc cttatattta caaatcagag caacatttca ttatccttat catatctttt
     1201 ttagtgaaaa aaagcaagaa aaaaccgaac aaatattcgc atttatcatt ttcttaaaag
     1261 ctgttttctc aaaagatcaa atccttgcat aaccgcatta tatctgatag cagcacgatc
     1321 tctggcaaag tgatattttt ccgaaaccac gccgtcttta gatgaaatag cgatccatac
     1381 tgttcctgca gattgacctt ctaacgtgtc cggtccagcc actccggtga aagctacact
     1441 gtaatctgtt cttgctatct cacgtgcttg aatggccatc tgctccacac attctttgct
     1501 gactgttcca tattctgcta atagctccgg atcaatctcc agaaaaccag cttttgtttg
     1561 gagcgagtag gtaacgaatc cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa
     1621 tgcggcttgg aaggcgccgg ctgtcaaact ttccgctgct gttacagtct gcttgttttc
     1681 tttcagtagt tccactacga cttttgctag cgaattatca tcgccgtatc cataaaaata
     1741 ttcccctact cgttcttgga ttttttcttc taatgctaaa agagcttgat tccctgcctg
     1801 aacatcattt gttttcactg tcagtcgtaa tgtcacttca tttggtttgg catatggtgc
     1861 gatcgttgga ttgatctgtg tctcaatcag atcttttagc tcagtgacca gtcgcgattc
     1921 accaatccca taaaagcgca aaactttaga aattagtttt tcttcagaag gaaaacgttc
     1981 ctctagtaaa ggacgtactt gttccacaaa cattggtttc aactcattag gcggaccggg
     2041 aagaaggata tacgcatgtg tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg
     2101 atttggtaga ggaaccccgc cttcgataat ctgggactgt tgcagattgt ttttggtcat
     2161 ttttcgatgg gttgtttcaa aatatgccaa taattttttg tatccttccg tattttgtat
     2221 gagagactta ccgaggtgag ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc
     2281 taatccgcca cataagagta tgagttcact tcttgtttca gctagagcaa tcaactcttc
     2341 taaacgctgc tgattatctc ctacaactgt ttgataataa acttcgatac ctagatcagc
     2401 tagctgttct gacaagaaag tggcattcgt attcacgact tgtcctagta aaagttctgt
     2461 tccgacagct attatttctg ctttcattac ttcccgctcc cttatatagt atacgctgta
     2521 tttttatatt tttttattta aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc
     2581 tccttgttcc aaataaacag tgttctcctg ccaaaacctc ggtcataagt cacaatatac
     2641 ataaaacatg aaaagctgtt ttttgaaatt atcccttgtg actcagagtt agacggcagg
     2701 ctcacaacct ctccactaaa cgatacttac attgatcctt tgaacacacc gctgtttttg
     2761 atgaagtaat ccacacctga atagatagta aagatcagac aagcataaag catgatttga
     2821 tctactggaa taccgatcag gctgaatggg atattattga taaacagcaa gataatagca
     2881 accatttgtg tagccgtctt gacttttccc ggccaagctg cagccatgac ttctccatgt
     2941 tcgacaagta gtaaacgcaa gcctgtgacg gctaattcac gacagacaat gatcgctact
     3001 acccaaccag gggcctttcc ttggtcaacc aacatgataa aagcagtcat aactagcatt
     3061 ttatctgcca acgggtccgc aaacttgcca aaattagtga caaggcctcg cgcacgggca
     3121 atttttccat ctaaccagtc tgtgatacta gctaaagcaa aaataactgc acctactagc
     3181 tgtgtgacag ccagtgtggt gtctcctacg ttaaattgtc cccaatctaa aggtaaacct
     3241 acaaccaaaa tgaaaatcgg gatcatacaa attctaagta cagttaattt atttggtaaa
     3301 ttcacactat cgactccttt cttccctatt ctacgtgact atcagcgaag tttccaccga
     3361 tattatttac ttttccaaat gaatttattc aaaggagtat ttttattgaa attgatcttt
     3421 ttgaattaac gaagaaggac cgcgacacag atttgtcacg atccttcttt tttataccta
     3481 caagtaaaca gatgcgtaaa ctaatttata cgatcattct gcgtattgaa gagtaaggtt
     3541 cacatttttt ggactctgat tgttttcccc tggattaact ggtacggatt ctccattcgc
     3601 tttgatcgtt gcattgcttg ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg
     3661 taacacggta ctctgtgttt catttgctgc cagtgtccct tgataaacat aggcattgtc
     3721 tactagcaca cctaaccata cacgattgac agcagtaaat tcaagggtta caggcttggt
     3781 agcgtctgtg atatttactg cgatggcact gccagaatct tctcctgtag ctattgccat
     3841 tttcttttct tcacttgatt ttgttgttga ttctactgta gaagactctt tggtctgcgt
     3901 gctttgctct gaggaagagg actcctcgac tgatccttct actttaagcg aagaagctgt
     3961 gtctccgatt attggatcag cttgacgatc ttgccaagtc atatagccca cgacaacgac
     4021 aatcgccaaa gcaaccaatc ctaataaaat gacaggaaga cttgtccaaa agcgacttgg
     4081 atgcgattct tcctgatgca ttgcttttct agaagtactt actgtttcag gctgcggacg
     4141 ttcagggacc gatcctgtca atttgctttt cccatcaaaa acatcgacta aatggtctcc
     4201 atcttctcct acagcttcag cgtattgccg aataaatgcc cgcacgtaaa atttaccagg
     4261 caattggtca aaatcatcat tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat
     4321 ctgctgtaat tcatctaaag atattttttt attcaggcgt gcttgccgta atttttctcc
     4381 gatggtttca aaagccactc gttcatcttc tccagtctgt ttgattctcc gaagcatctg
     4441 ctgttgcaac gaaagacgtt atgtttgggt gctgtgacaa aagtcatgtc acagctcctt
     4501 ttttccgaat aaacattgtt ctcctgccaa ctcctcggtc acaagtcaca atatccaata
     4561 aatataagaa gctgtttttt gaacttgttc cttgtgactc ggagttaaac ggcaggctca
     4621 cgacctcttt cttaaacggt gttcaatcag ctgttcctcg gtattagctc aaaaaccggc
     4681 aaaatataag gagatatttt tccgtatgtt tctctaatac tcaggtcaaa acgctgattt
     4741 cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa
     4801 gttaaaaaca ttaagaagct gtttttactt ggctgttctt attacttgat gcagggcagc
     4861 tttttcacaa cctcttggta cacggtgttc cagaagtagc ttcttcggca ataagccgaa
     4921 atatcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
     4981 acttctgtca caacctcttt gattacttcg tttgatttta ttttaatata agttgattaa
     5041 aagagagcat gatttgtttt attttctcac taatttttcc aacacaacta agaataccat
     5101 aaaagctgat tttggaggag gttcctacta caagtctaaa acgaaattta agacttttcc
     5161 tcataaaata ctggtttatt ttggtctcat gtaaaactcg ctgaaggcct cttcacttat
     5221 aaatgcttcc ccagctgcca gtacgtcttt catttggatc gaatcgatga tttcaggaag
     5281 atcaaataaa gttcgatcgc caaataaact ttgcgtaaat tgattggcaa tatattcgat
     5341 cgaatttaat gattggaaat attgtccaag cattttcttc ttcagcaaat cgagatttgt
     5401 ttctgacact tccggatcat cagcgaatcc aagaatgatt tctttgactt tttctgctgc
     5461 tttctctggt tcgtccgtat ctccgctgaa gtccgcaaag tgaaattcac gatccaagct
     5521 gaattcaaaa ccaaagctgt catcgataat tccttgattg tacatcgcta gatagtttcg
     5581 agaagtattg cctaaaatca tttggaacaa cagattgatt gctgttttat agcgtaacag
     5641 ttctgttcct tcttgcggca atgtgtctaa tcctttaata ccaagtacaa atttatctcg
     5701 tgtaatggct gcttccaatg cactttgttt gatgatttct tttgtgtttt cagggaaata
     5761 acgtacaatt tcttgctttg gagggaaatt ttttgcttct tggttttccc gaataagttt
     5821 catcaatttc tcaggctcca tcttaccaac tacaaataaa accatattgc ttggctgata
     5881 aaacgtccga tagcacgtat acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt
     5941 gcccgcaata tcgatatgca atgggtgtgt aggatacaag ttatttagta tgccaaaaaa
     6001 catccgccag ttagggtcgt cttcatacat ttggatttcc tgaccgatga ttcctttttc
     6061 cttgtttaca gtttcttctg tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag
     6121 attcttttct acctgatccg tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa
     6181 agcattggcg gaagctcctt gtttcccaaa taattgaaag acatcaccat cttctttttc
     6241 aaacaactta tgttctaaaa aatgggcaat tccatctgga actttcacct tttctttttc
     6301 accataggga ataaattcgt tatcgatcga accataattc gtactgaaca aaccatatgt
     6361 cttgtgatag tcatttttag gcaacaaata cacggtcaaa ccgtttggca gtacttcatg
     6421 ataaagtgtt tcattgattt gctcatattc tgttttattc atttgcattt cctcctgcta
     6481 aaaagaagat tgcctgcaat tcgatttgct cagctacttg ttgtatctct ttgatcgtta
     6541 cgctctccat acgtttcaac cattcttcat cagataattt tgtttccggc agccagctgt
     6601 ccaaaaaaga agcttcgatt gccgcttgcg gagagtctag agaaagcaga tattggtttc
     6661 tcagcattgc ttttgtctga gacagttcca actctgttat ttcaccattt cgcaaacttt
     6721 ctaattgctc gtgaatcaga tgaagtactt gatttcggtt cttttcatcg ataccggttt
     6781 ggacactcat gaatcctcgg aacgtatcca cgctactaga ggcataataa gctagacttt
     6841 ctttctctct gacattcatg aaaagtttag agtgaggaaa accgccaaat aaaccgttaa
     6901 agaccattag cgcaaaacgt tctggttcat cgtaatacac atttgtctga taagctaggt
     6961 tcagctttgc ttgaacgatt ggctcacgta cttgtcgttc cttcacgata ttcacttgag
     7021 gttgggtata aaacagatct ggataaactg tttcccgcga ctcaaatggt aattgcccaa
     7081 ctgcttcagc tgctttgtcc ggatcaacat cgccgacaac aaaaatgtcg atttgatcat
     7141 ttgccatcat ttcttgatag taggacgcta gcgaactagc agtgatcttg tccaaatctt
     7201 cgactacgcc caaccctggg atcttttgtt ccgggctgtt ttcaaaataa agttcttgca
     7261 aggcatagga agcaaaagtt tgtttatctt ccttcaaact ttccaaatat agacgcagat
     7321 tgttcttttc caagtcaaac gtcaactgat caaactgatg atttttaata tttggataaa
     7381 ataaaacctc ttttaaaaaa tctaccgctt gagaaaaaag gtttggatca ttgacatatt
     7441 caccattcac aaaactgatc cctgcgttta gccaatgtaa atttcctttt tttctaactg
     7501 ataatccaaa gcttgcgcca taaagctctg caagttgcgc acttaacttt gtctgatccg
     7561 aataattcaa actatttgtt tccaacatgc tgctgagaag tgaacgtttc gtgatgactt
     7621 cttgttgcag acgcgcagta aaacggataa aaatccggat cgttttgtat tgttttgttg
     7681 gtaagacatg aagattcacg ccttctgcca attgatacac catatttata tgctcctttt
     7741 cacagttgat tgcccgctta ctcttttaat gggtcaacct gcctttttta cttattcctc
     7801 tgcttgaaat ttataagttc aggcaattcg ccgttttttc taaatgatgt tacactttat
     7861 agtatagcat agatgttttt tatcaaaaga aacagacggc taaaagcgta tattgttttt
     7921 ttatttttta tttttcgata tactaaagac aaataaaaga agggagggtc attcatgatc
     7981 aaagaattca aagagttcat catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt
     8041 attggtagtg cgtttacaag catcgtaaac caagtggtgc aaggcttgat cacaccacta
     8101 ataggttggt ttgtcgcaat gatcacaggt acttctgatt tagaaggagc actttcaatt
     8161 cttgactggt cgccgacaaa aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc
     8221 attacatttc tgatcactgg ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg
     8281 aagaatttac gctccaaaga agagcaagtg gaagtggaag aaactcctac tgcagaagat
     8341 tatcttagtg acatacgcga tcttcttgcc aaacaagtgg cagaagacaa tacacatcca
     8401 gtagagcaaa caaatctttc cgatacttct aaataataaa gaatctaaaa aaaaagaaag
     8461 ccgattttca gtgtcggctt tcttttttta gattcttaac ttgctacttg ttcctttttc
     8521 atgtcgatat gtggaatatc atcttccaaa tacacatctg agattgcctt gaatccaaac
     8581 gactcataaa aagcttgtag gtattcttgt gcgccgatca cgattggttt ttctggaaaa
     8641 tttttctgga tcatgttaat cgtttcttga acaatctttc ttcctaaacc atctttacgg
     8701 ttgtcctgat gaacaaggac acgtccaaaa tggatactct ccgcttcttc atatactcgc
     8761 gtgtaggcaa ggatcttccc atcagtatct tgaaaaaacg tatgccaagc ttctccatca
     8821 atgtcatcaa cttcttgata aggacactgt tgttccacaa caaaaactgc gattcgttct
     8881 ttgacgatcg cgtaaaactc tgctgttgac aattcttcaa atttctttac ctgatattcc
     8941 actaagcaaa cgccccctgc ttttttattc ttagcatacc gattcgcaca caaaaaagca
     9001 aacgaacctt acgaatctca ggctcgtttg ctttttaaac tgcttcagtt aaaaattcga
     9061 tctcaactgt ccgagtaagt acatctgaat agctgtaaga tactcgttca aaagaatttt
     9121 cttcaggatc caaatcaaca acgaatactg aaggataggt ttctgttaaa acgccgcggc
     9181 gttctgtttg acgctttctt cctgtctgag caaccaacat gattgggctg ccgatacgac
     9241 cttctaaatc tttcttgatt gaagctaaag ttgttggcat acagttcacc tctactatca
     9301 actataccac atatttttaa aattttcaag tttaccataa aaaaaaaaag aaatgcaata
     9361 aaaataaata agaagctcat taagattttt ctaaattatt cctcattata aaatttcaag
     9421 tgttttttta ttttgagttt tactctcccg taagcgctca ttacttgctt gtgatccatt
     9481 gctaaacatt cagccatgtc tacaaaatca acgccttgca tataataatg aaaaatttta
     9541 gcttctattt ttgaaaactg gatttcgcaa ttttcgattg cttcttttaa taaaagatgc
     9601 tgaattgcta aaggttcttg caatgtgatc tctgctaaaa agtctggtcc ttctgtctct
     9661 attttttggt cgattgagac ttcatctttt tgatcacgac gcttcagcgc gttctgcttc
     9721 ctcaacagag aatagatttg attgcttata acgcttttat aataacttcc aaagctaaca
     9781 cctttgcttt tttcgtaagt tctcaatgcc tgatataaag cgatccttgc ttcttgttcc
     9841 caatcctcag aaacataatt tcttaaataa taggtgtttt tcaaacgtag taaaagtgga
     9901 aaatattttt gatacagggc agcaaatgct tcttcgtcac cattacgtgc agcgcgcaat
     9961 aactcctcag acataaaata attcctccat aaattcgttt atggaggaat cttatcatca
    10021 atgattcatc gaactgatta gaacgatatg ttatttattt tttctcatta aatcgtccaa
    10081 tttttgggac aattttttta actgctcttc attccatgga gaattacgtc ggaaatcaat
    10141 aaaatgcata tctttatttt cttgggctat tgttttttcc gtttttttta tcgtcttata
    10201 taattcataa gcagaagttc gtaatgctcc ttgggaaaag atcatccact gttctgctaa
    10261 atcgcttgtc gccactgtca cttgtgtcaa acggtcattc agctgaccag cgattctctc
    10321 gatataactg tcagccgttt cgtcttcttt cgtaaaaaca acatccaact ggtatttctt
    10381 ataattttgt tggatgcccg gaactagttg ggcatcgaag acaacgatga cttccattcc
    10441 ttcatatttc gcataatttg ataaacgatg taaaagagct tctcttgcat ccgccatttt
    10501 gtcctgcttt ttaagctgga caagttccgg ccacgaacca atcatgttgt agccgtctac
    10561 aatcaacagc tgttttttca tggtctactc cccctgacgt tgggaaaaag cctgatacat
    10621 aagcaaacca gcagcaacac tggcatttaa gctctggacg tgcccggtca tcgggatcgt
    10681 cagcaattca tccatttctt ttttcaatcc tggactcatt cctttgcctt cattaccaat
    10741 gactaaagca attgatcctt tggcattcca tttttggaag tttgtgccgg tcatatctgt
    10801 gccaaatacc caaaatcctg tttctttgag ctgttgcaca gcttgtacca aattcgtcac
    10861 gcgtgcaaca gggacatgtt cgactgcccc agtggaagtc tttgttacga taggtgtgat
    10921 tccaactgct cggtgttttg gtatgatcac accatcgacg ccacttgcgt ctgctgtccg
    10981 taaaatcgac ccaaaattgt gtggatcttc gatgctatcc aaaatcaaaa agaaggggtc
    11041 ttcttttttc tcttttgttc gttcaatcag ttggtcaagg gataaatatt cataaggtgt
    11101 aatcgcaaga acgactcctt gatgcacgcc gtgatcgctc aaagtttcta atttttgttt
    11161 aggaacccat ttgactggca cagcttgttc acgtgcgatt tctttcagtt cttctacttt
    11221 ttctcctcgt gaatcttctt gaagaaagag tttattccct cgcccttgtt gtagcgcttc
    11281 tactgcggca tggtgcccaa agacgaaatt atcttctaat gcttcttcat tttgatcaga
    11341 aactgactga tttcgtccag tttgcttttt atttgaatat ctattttgtt ttttgtcttc
    11401 aaatccacgt ttcgattgat gtttgtttgt tctttcttta cccatttttt tcaccaactt
    11461 tctgaataca ccagcggatc aattcttcca tccgctcggt ttgttttgtc aaatgcagat
    11521 agcccatgag cgcttcgaac cctgttgcga cccgatacgt cgtaacatca gcattttttg
    11581 cgctcgtatg gcttttagca tttcttcctc tgcgataata aagttcttct tcttctgtca
    11641 aaagatgttc ttccagcatt tgctgaatca aaaaggcttg tgcattcgca gaaacataat
    11701 tcgttgccgc acgatgcagc ttattgggtt ttgtttctcc ttgatcaatc aggtggtcac
    11761 gaacatagat ttcgtaaatc gcatctccaa cataagccaa ggctaaaccg ttcaattgtg
    11821 tgtaatctct catgcttctc ttctccatcg ggttccttgc ggtgtatctt ctaataagat
    11881 ccctttttct ttcaacaaat cacgaatttc gtcgcttcgg acaaaatcac gattgcttct
    11941 tgcttgattt cgttcttcga tcaattgttc aatctcttca tctaataact cttctgctgt
    12001 aaataaaata ccgaagatcg aaagccattg cgagaataaa gcttctcctt gtgccaagac
    12061 agcggcagaa actttcggct ggtctagata agtgttcagc cacttagcaa gctcatagac
    12121 cactgtgatc ccatttgctg cattaaaatc atcgtccatt tcttcgatga atcgctgttc
    12181 taaattttct aacaaagcga ttttatccgc atcatctgcc aaattctctg aggcagtttc
    12241 ttggcggaat ttcaaattgt caaacgtatt cttcagtcgt tgtaaattgg tggctgcttc
    12301 tttcatcgtc gtttcactgt agcggatcgg acgacggtat tgtgtggtcg ccatgaagaa
    12361 acgcaagact tgtggatcaa cgtttttgat catttcatgt acagtgatga aattccctag
    12421 agatttactc attttttcat cgtcttcgcc aatcgttaca tagccattgt gcatccagta
    12481 attagcgaat tttttccctg ttttcgcttc actttgtgcg atttcatttt catgatgagg
    12541 aaattctaaa tcttgcccgc caccatggat atcgatcgtt tctcctaaat gttttgttgc
    12601 cataacggaa cattcaatat gccaaccggg acgtcctttg ccccatggtg aatcccaaga
    12661 aatctcgcca ggttttgctt gtttccataa agcgaaatct aatgggtctt ctttttgctg
    12721 ctgttcttcc cctgtacgct gacttgctcc gacttccagt tcatcgatgg attggtcact
    12781 taatttccca taattttcaa atttacgggt acggtaatac acatcacctg aagattcata
    12841 agcatagcct ttttcaacca acacttgaat aaatgctaaa atatctggca tatgatccat
    12901 gactcttgga tgcagtgtag caggttcgac attcaacgct ttcgtatctt cttcaaatgc
    12961 tcggatgaaa cgatcagcaa cttctggtgc tgtaatacct aattcgtttg ctgtacggat
    13021 gattttatcg tctacatccg tgaaattcga tacgaaatcg actttatagc ctcgatattc
    13081 aaaatagcgt cgaattgtat cgaaagcaat ggtgctacga gcatttccga tatggatata
    13141 attatacaca gtcggtccgc agacatacat accgacatgt cccttttgta tagggatgaa
    13201 ttcttctttt tttctggtca atgtattgta gatttttatc attctgatct cctcactttt
    13261 tctcctttga ttcgaacgac ttttgctggt actcctaccg ctgtagcgtc gtctggaaca
    13321 tctttcaaca caacggcacc tgcaccgatt ttggcgtttt ttccgatcgt taccggtcct
    13381 aatatctgcg cattcgcaga gagcatggct ccttgtttaa cagttgggtg tcttttaccc
    13441 gtttcttttc ctgtgccgcc taatgttacg ccatggaata aaatcacatc gtcctcgatt
    13501 tctgcggttt cgccaatgac gattcccatt ccatggtcga tgaacacgcc agttccaatc
    13561 gttgctcctg gatggatctc aacaccagtt aggaaacgcc agaattgagc attgatcttc
    13621 gccaataaaa agagccggtg ctgatataaa aaatgagaga aacgatgcca aaacaaggca
    13681 tgaaaaccag ggtatgtcaa aacgatctca gctgtcgaac gtgcagccgg atcgtttctc
    13741 tttgctgcat caatcgctcg ggtaaaccaa ctcatgactt tccactcctt tcgtaagaat
    13801 tttatgtagg aaatcatctt cttataaaga aaagaagcgc cttttaaata agtttcctta
    13861 tcaaaagacg ctttccgcgt ggttccactt ttcttcataa tcaatgatta tctctgacag
    13921 ctaacgcctg ttatacgttc ttggctactt tgtttcctat aaaacagttt ttcctgtttt
    13981 ctgtgtttcg ccaagaccac tcccagaggc atttcggcaa ccattcattg ataccttgca
    14041 ccacccggta tctctcttgt aatgatcagc tgcttacttt tttctgttca tcgtgtttag
    14101 ttcagtactt gatttaaatg tgctaatgat ttttcacgac caagcaattc gatcgtttca
    14161 ggcaattctg gaccatgcat ttgtcctgat acagctacac ggattggcat gaaaagattt
    14221 tttcctttta cgccagtttc tttttgaaca gctttgatgg cagctttgac agttggagca
    14281 tctactactt ccatttgttc tagctgtgct ttgaatgcgt ggagaacagt aggaactgtt
    14341 tcaccagcaa gtacttcttt tgctgcttca ttcaatacag gatgttcatt gaaaaatagc
    14401 gtagacagat caacgatttc tgcagcatag ctcatttgag gttgatacag gctaacgatc
    14461 ttcttcaccc attcgattgt cttttcatct gggttttctg ctacacgtcc atcagcgatt
    14521 agataaggca gacacatgtc agtaaatgca tctagatcca ttgctttgat gtaatggttg
    14581 tttacccatt gcagtttttt cgcatcgaaa gctgcgggtg atttacttag gcgttttgga
    14641 tcgaacattt tgatcaattc ttggtgttcg aagatttcgt cttcgcccac aggtgaccag
    14701 cccaataatg caataaagtt gaacattgca tctggtagat agcctaattc gcgatattgt
    14761 tcgataaatt gcaagattga ttcgtcacgt ttgcttagtt ttttacctgt ttcactgttg
    14821 atgatcaatg tcatatgtcc gaaggttggt ggtgtccagc caaatgcttc atagatcatc
    14881 agttgttttg gtgtattggc aatatggtca tctccacgta aaacgtgagt gattttcatc
    14941 aaatgatcat cgaccgcaac agcaaaatta taagttggca taccatcgcg tttttggatg
    15001 acaaagtcgc caccgatatt atcggattcg aacgaaatag cgcctttcac catatctgta
    15061 aatgcgtatt ctgtattgcg aggcacacgg aaacgtacaa cagattctag accttgtgct
    15121 tctttttctg cttgttcttc aggtgtcaag ttcgcacatg ttcctgcgta atgaggcatt
    15181 tctcctcttg cgcgttgtgc ttcccgttct gcttctaatt cttcttctgt gcaatagcat
    15241 ttgtatgctc gattgctagc aagcagctga tcaatcagcg gttgatagat ttctttacgt
    15301 tcagattgac ggtaagggcc gtattctcca ggattttcag gagattcatc ccaattcatt
    15361 cccaaccaag caaggttttc caactggctt ttttcgccat cttcgatatt acgtttttgg
    15421 tcagtatcct caatacgaat aataaagtct ccatcattgt ggcgggcaaa caaataattg
    15481 aataatgcag ttcttgcatt tccaatatgc aggtgcccag ttggacttgg tgcatagcgt
    15541 acgcgtactt tcgtcatttt cttcctcttc tttcaaacta gactttgact aacagcaaaa
    15601 attccgtcag ttacggcttc tcaaatatca tttatttaaa gccactcagc acaaatttta
    15661 ccttttctaa ggtaaatagt aaagccaact ggttgaattt ccttaccagt tggctgaatt
    15721 tcttttttat tttgattgtt tagtcttttt ctctgtctct tttttattgt cgattcctcg
    15781 tcctgcatgt gcaggtttag caaagatcat gcgtccagca gctgtttgaa gggcacttgt
    15841 tacaacgact tcgatacgtt cattcatgta atgctgtcca tcttctacga caaccatcgt
    15901 gccgtcatca aggtaggcta ctccttgttg gcgttctgtc ccggctttta ctactagaac
    15961 attcatcgtt tcaccaggga tgacgactgg ttttacggca tttgccaacg cattgatatt
    16021 aagaacaggt acattttgga attcagagac tttattcaag ttatagtcat tggttacaat
    16081 gacaccatct aaaagcttag ccaatttgat caatttgctg tctacttcac tgatatcttc
    16141 aaagtctcca tcgtacattt cgacagatat accctcttct ttttgcaagg catttaggat
    16201 atctaatccg cgacgaccac gcacacgttt caaactatcc cctgagtccg cgatgtattg
    16261 tagttcgtaa agaacaaaat tagggatcat caaggttcct tctaagaatc cagtctttgt
    16321 aatatcataa atccgaccgt caatgatcac gcttgtatcc aagattttat atttatggaa
    16381 attttctcct gattttcgtt ctaacagttg actctcaact tgactagctg tttcttgctc
    16441 gatttttttg cttcgtgaac caaaaacttt cttccattct tcgattcgtg tcgttcccat
    16501 tctcaatccc agatagccta agatgatcat cagtaaaatc ggcaatacac tattgacgaa
    16561 tggaatttcc atattgtaaa gaggggtcga gatcacaaca cctaaagtca atccgatgat
    16621 cgcaccgatc gcaccgaaga gaagataagt caagctgacc tcacttagtt tttgttctac
    16681 ttgtttgata atagctgaga tatgtttggc caacagcaaa gataataaaa agaaaataag
    16741 tgcgcctaac aggctgttgg taaatgtatt attcagccat ccgtttgact gttgaccgat
    16801 tgcctgccaa gctgagggta aaaatgaaat acccaagctt gcgccgacaa tgatcatcag
    16861 caaagtaatg acacgttttt gcatcgtttt tcctccgttt cgacaatcat ttattagtta
    16921 gcggaacaca cgcttcaacg tttcagccaa agttgctact ccaactatct cgattccttt
    16981 tggcgcttcc cagttaccta agttgttttt aggtaggtat acttttgtga agccgagttt
    17041 ttggacttct ttgactcgtt gttcaatatg actgacacgt ctgatttctc cagtcagtcc
    17101 aatttcaccg atgaaacatt ccgttggctg tgttcctttt tctttatagc ttgaagcaat
    17161 actgacagcg atagctaaat caatcgctgg ttcatttaat tttaccccac ctgcagcttt
    17221 taagtaagca tcttgattct gaagaagcaa cccagctctt ttttccagca ctgccatgat
    17281 cagagatacc cgattgaaat caagacctgt tgttgttcgt ttggcatttc cgaacatagt
    17341 aggtgtgact aatgcttgga tctctaccaa aattggacgt gtcccttcca ttgccacaac
    17401 gatagcagag ccagttgcac cgtctaggcg ttcttctaag aaaatctgtg aaggattagc
    17461 gacttcttcc aatccatgct catgcatttc aaaaatccca atctcattcg ttgagccaaa
    17521 tcggtttttt actgctcgca aaatccgaaa actatgatgt tgttcacctt caaaatataa
    17581 caccgtatcc accatatgtt ctaacattct tgggccggcg atcgagcctt ctttggtaac
    17641 atgaccgacg ataaaaatgg ctatcccatt tgttttagcg gttttgagta gttctgcagt
    17701 ggtttctctt acttggctga cacttcctgc cacacttgtg atgtctggct gcgtcatcgt
    17761 ttggatggaa tcaatgataa cataatcagg ggagatatgt tcgatggcac gactgatttc
    17821 gttcatatct gtttcagcgt atagataaaa ttctgtgttg atacttccga ggcgttccgc
    17881 gcgtaatttt atctgctctg cgctttcttc gccggataca tataagactt ttccgcctat
    17941 ggctgctaat tgctgagaaa cttgtaacag caaagtggat ttcccgatac ctggatcgcc
    18001 accaatcaag accatagacc ctggcaccac acctccgcct aacacacggt tcagttcttc
    18061 taattttgtt ttgatccgag gttctttttt agggacgacg tctgcgattt ttgttggttt
    18121 tgctttttct cctgtcaaac ttgtccgagt tctgcggtcc gttgtatctt gttcgatttc
    18181 ttcgaccatc gtattccatt ttccgcaatt tgggcagcgt cccaagaact taggcgacac
    18241 atacccgcaa ttttggcaaa caaattgtac ttttgatttt tttgccattg taccgcctcc
    18301 ttttatccta atcatgagac attaaatcaa gcttaagtat ctttaacgtt ttatttactg
    18361 gaagaaccaa atccgccttg acgttgagca gtcgtttgat cttgatctgc ttttaaaaac
    18421 ggcaaaaaga tcccttggcc gatacgctcg ccttttttga tcactacatc cttgaaacca
    18481 aagttggtga attggaacat gatatggcct tcatttgcct cattattgta ataatcactg
    18541 tcaatcacac ctactccgtt tgtcaacact aaaaaccgct tcaacggatt gcttgaacgg
    18601 ttagcaagct gcaaaaattc atcttctccc atgtaagctt tgattccagt aggcacgagg
    18661 gttggtttca attgtttttc attttctgat acatcttttt tcaatccatc tgcgactgtt
    18721 tccaacccct ttttccagcc tgctttccag atacttggaa tcacgacctc ttctgctgct
    18781 tcaaaatcat agccagcagc atgatgtgta gcgcgctgcg gtagattgat attttgatct
    18841 ttatatttag aaataacttc aaatcctctt tttctcaccg gttttcctcc ctaaataaac
    18901 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
    18961 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
    19021 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
    19081 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
    19141 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
    19201 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
    19261 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
    19321 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
    19381 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
    19441 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
    19501 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
    19561 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
    19621 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
    19681 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
    19741 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
    19801 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
    19861 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
    19921 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
    19981 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
    20041 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
    20101 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
    20161 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
    20221 gattgagaat cggcaaaaag cgcgtgccac taggcgtgat cggcatcatt tacgaatctc
    20281 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
    20341 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
    20401 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
    20461 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
    20521 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
    20581 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
    20641 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
    20701 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
    20761 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
    20821 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
    20881 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
    20941 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
    21001 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
    21061 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
    21121 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
    21181 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
    21241 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
    21301 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
    21361 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
    21421 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
    21481 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
    21541 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
    21601 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
    21661 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
    21721 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
    21781 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
    21841 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
    21901 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
    21961 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
    22021 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
    22081 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
    22141 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
    22201 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
    22261 ctcttggtaa acggcgttca atcagctgac cctcggtatt agctcaaaaa acggtaaaat
    22321 atgagaagct atttttccgt tttgtttctc taatacttag gataaaacgc tgattttaca
    22381 acctctgtta aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa
    22441 tatgaggaac tatttttccg tttgtttctc taatactcag gacaaaacgc tgattttaca
    22501 acctcctgat aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
    22561 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
    22621 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
    22681 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
    22741 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
    22801 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
    22861 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
    22921 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
    22981 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
    23041 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
    23101 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
    23161 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
    23221 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
    23281 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
    23341 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
    23401 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
    23461 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
    23521 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
    23581 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
    23641 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
    23701 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
    23761 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
    23821 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
    23881 tcaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
    23941 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
    24001 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
    24061 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
    24121 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
    24181 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
    24241 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
    24301 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
    24361 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
    24421 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
    24481 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
    24541 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
    24601 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
    24661 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
    24721 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
    24781 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
    24841 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
    24901 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
    24961 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
    25021 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
    25081 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
    25141 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
    25201 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
    25261 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
    25321 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
    25381 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
    25441 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
    25501 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
    25561 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
    25621 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
    25681 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
    25741 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
    25801 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
    25861 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
    25921 cagtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
    25981 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
    26041 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
    26101 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
    26161 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
    26221 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
    26281 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
    26341 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
    26401 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
    26461 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
    26521 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
    26581 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
    26641 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
    26701 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
    26761 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
    26821 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
    26881 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
    26941 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
    27001 aacatagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
    27061 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
    27121 aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact
    27181 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
    27241 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
    27301 ggcatcttcc ctgattcgta agcttggttg tagatttctt cgccatattc gtaacattgc
    27361 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
    27421 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
    27481 ttcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
    27541 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
    27601 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
    27661 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
    27721 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct tgattttctt
    27781 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
    27841 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
    27901 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
    27961 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
    28021 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
    28081 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
    28141 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
    28201 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
    28261 tattgattct tatcaaacta tagaagatag ctcttctgca gtagataatt ttacaccgcc
    28321 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
    28381 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
    28441 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
    28501 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
    28561 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
    28621 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
    28681 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
    28741 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
    28801 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
    28861 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
    28921 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
    28981 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
    29041 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
    29101 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    29161 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    29221 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    29281 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    29341 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    29401 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    29461 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    29521 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    29581 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    29641 aattgtattt tctgagacag tagctgactg caaagtactt tctgtatcgt tttcaattcc
    29701 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    29761 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    29821 tttcatcttt tttctccttt gaattatgaa acttttccta aataacag
//
LOCUS       DACDMM010000019        26540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDMM010000019 DACDMM010000000
VERSION     DACDMM010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.019"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            80..268
                     /locus_tag="IWD16_000646"
     CDS             80..268
                     /locus_tag="IWD16_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087730.1"
                     /translation="MDIYKKLASKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPAD
                     FYRKMQIINYQPLAETHA"
     gene            complement(912..1865)
                     /locus_tag="IWD16_000647"
     CDS             complement(912..1865)
                     /locus_tag="IWD16_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9087731.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1888..2643)
                     /locus_tag="IWD16_000648"
     CDS             complement(1888..2643)
                     /locus_tag="IWD16_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9087732.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2640..3599)
                     /locus_tag="IWD16_000649"
     CDS             complement(2640..3599)
                     /locus_tag="IWD16_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9087733.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3592..4542)
                     /locus_tag="IWD16_000650"
     CDS             complement(3592..4542)
                     /locus_tag="IWD16_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9087734.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4692..5144
                     /locus_tag="IWD16_000651"
     CDS             4692..5144
                     /locus_tag="IWD16_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP9087735.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(5233..7500)
                     /gene="gshAB"
                     /locus_tag="IWD16_000652"
     CDS             complement(5233..7500)
                     /gene="gshAB"
                     /locus_tag="IWD16_000652"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP9087736.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7687..8685)
                     /gene="ftsY"
                     /locus_tag="IWD16_000653"
     CDS             complement(7687..8685)
                     /gene="ftsY"
                     /locus_tag="IWD16_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP9087737.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8696..9505)
                     /locus_tag="IWD16_000654"
     CDS             complement(8696..9505)
                     /locus_tag="IWD16_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9087738.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9514..13095)
                     /gene="smc"
                     /locus_tag="IWD16_000655"
     CDS             complement(9514..13095)
                     /gene="smc"
                     /locus_tag="IWD16_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP9087739.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(13115..13801)
                     /locus_tag="IWD16_000656"
     CDS             complement(13115..13801)
                     /locus_tag="IWD16_000656"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9087740.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(14149..15930)
                     /locus_tag="IWD16_000657"
     CDS             complement(14149..15930)
                     /locus_tag="IWD16_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9087741.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15956..16870)
                     /locus_tag="IWD16_000658"
     CDS             complement(15956..16870)
                     /locus_tag="IWD16_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9087742.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16897..17859)
                     /locus_tag="IWD16_000659"
     CDS             complement(16897..17859)
                     /locus_tag="IWD16_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9087743.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17859..18803)
                     /locus_tag="IWD16_000660"
     CDS             complement(17859..18803)
                     /locus_tag="IWD16_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9087744.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18804..19808)
                     /locus_tag="IWD16_000661"
     CDS             complement(18804..19808)
                     /locus_tag="IWD16_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9087745.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(20076..20318)
                     /gene="acpP"
                     /locus_tag="IWD16_000662"
     CDS             complement(20076..20318)
                     /gene="acpP"
                     /locus_tag="IWD16_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9087746.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(20440..21441)
                     /gene="plsX"
                     /locus_tag="IWD16_000663"
     CDS             complement(20440..21441)
                     /gene="plsX"
                     /locus_tag="IWD16_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP9087747.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(21490..23526)
                     /gene="recG"
                     /locus_tag="IWD16_000664"
     CDS             complement(21490..23526)
                     /gene="recG"
                     /locus_tag="IWD16_000664"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP9087748.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(23643..25325)
                     /locus_tag="IWD16_000665"
     CDS             complement(23643..25325)
                     /locus_tag="IWD16_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP9087749.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(25377..25739)
                     /locus_tag="IWD16_000666"
     CDS             complement(25377..25739)
                     /locus_tag="IWD16_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9087750.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            26009..26197
                     /locus_tag="IWD16_000667"
     CDS             26009..26197
                     /locus_tag="IWD16_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP9087751.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7320 a         5746 c         4684 g         8790 t
ORIGIN      
        1 tatcgaagaa acacaaaata tcgagggttc ttatattgaa gtaaaaacac tctttgtcaa
       61 tgaagataaa accaaagcat tggatattta taaaaaatta gcttcaaaaa aaacgaatag
      121 ttttggattg atattaagcg agtataaaat caaagcagaa gagagttatt tctatcaatt
      181 attaaaacgg tggagtaagc ttcccgccga tttttatcga aaaatgcaga tcatcaacta
      241 tcaaccgctt gctgagacac atgcctaaat agaggttgtg aaatcagtgc tttgagctaa
      301 taccgaaggt cagctgattg aacattgttt agaagagatt gtgacaactg cgatttgctt
      361 tgagtaataa gaacgctgga cagaaaatag cttctcatat tttctagtct agtgggctta
      421 ttgccgagaa gcagcagatg gaacatcgtt tagaagagat tgtgacaact gcgatttgct
      481 ttgagtaata agaacgctgg acagaaaata gcttctcata ttttctagtc tagtggactt
      541 attgccgaga agcagcagat ggaacatcgt ttaaagaaga gatcggtaca aaataaaaat
      601 atctaaattt aagttttatc taagtttcgc tttgtacctt tgattcaaat caaaggaggc
      661 gatcttgatg ctgaaaaaat ggatcacact attttttgcc actccccatt ctagaatcaa
      721 cccgtatgat gtagataatg aattcacgtt gaaaaaagat ttctcaaata aataaaaacc
      781 atccgtaaaa tcatcaatac gattttacgg atggtttttg ataacttctt tgaggatagg
      841 ctcttgctat ttaagaatgt aagaaacatt attgttacat agtatgagag tttaatcctc
      901 tgttatttat gctatttaaa tgcttgattc acatcttcga tcatcagttt cattgattct
      961 aaaccgcccc cgcttaaata ccagacatct ggttcaaggg agatgatctg ctgattttta
     1021 ccagcattcg tttgtgctac cagttcattg gcagaaatat catctttgct gtcgtctcca
     1081 ccaatagctt ttgttctgtc tactacaaat aagatatccg gatttttttc cagcacatat
     1141 tcataagaaa cactttgacc gtgagtggat gcttcgattt gatcatctgc ttgttcaaag
     1201 ccaaataaat catggatgaa accgaatcta gaaccagatc cgtaggctga taattgccct
     1261 tcattcacaa gtgtgactag tgcttttttg tcagtagctt ctgctttctt tttcgtctga
     1321 tcgattgcac tagataattc ctctaattga gttttagctt cttcttgctt accaaagatc
     1381 tcccctaaag cagtgacatt ttgctggaaa gattcccatg gatttttcgc atctaaagaa
     1441 aggaaaatgg tcggagcaat ttgacttaga tctttttgaa aatctctttg tcggccagaa
     1501 atgatgatta gatctggctg aagctgatta attttttcta agtctggttc ttttattcca
     1561 cctgctgatt ctactttttt ataggatgaa agatactcag gaaggctgct tgtagctgct
     1621 ccaactacct tatctccaac accaagagca tccatcgtat ctaaagaacc attgtcgaat
     1681 acgacgactt tttctggatt atttggcacc tcgatttggt ctccattact atctgttatc
     1741 gtcaatgtag tttcagtttg tgtgctgctt ttttttgtgt ctgcctgact ggtgttgttg
     1801 ctatttccac atgctgctaa accaaatgat gctagaatca agatacctac tactaatttt
     1861 ttcatctctt ctctcctaat gttagtttta gaaataaaga cagaatctgc ggccttcaat
     1921 ctcacaaatc cgtatgtcca tttcgtataa ttcatccaat acatcttttt ttatcatttc
     1981 ttgggtcgtt ccttgcgtaa ataactgtcc atctttcatt gcaacaattt cgtctgcata
     2041 acttgcagca aagttgatat catgaagaac aataacaatt gttttcccga tttcttgtac
     2101 taaatttttt aaaatctgca tgatttgaac cgcaaaattc atatctagat tgttcaaagg
     2161 ttcatccagt aaaatataat ccgtgtcttg cgctagcacc attgcaatat acgcacgttg
     2221 gagttgaccg cctgaaagag tgtcaatcga ttgatccgct atttcttcta acccaaggtg
     2281 ttccagtgat tcctgaacga tttcctcgtc ttttgtcgtc aattttcctt tcgtataagg
     2341 aaatcgtcca aattctacta attctttcac ggtgatttgg acatttattt cattcgattg
     2401 tcgtaggatc gataattttt tggctaattc attggatttc caagccttta cttcgttatg
     2461 gtctaaatag atttcacctg tatcttttgg tatcaaacga ctgatcatcg acaacaccgt
     2521 tgactttcca gcaccatttg gaccgataaa agcagtcaat ttcttttcag gaatcgttaa
     2581 ctgtacatct ttaaccactg attttccatg gtacagttta ttgacctttt gtaaatcaat
     2641 cataggatac tcttcctttc gcgaatcaat tttgtgacaa aataaattcc tccgacaaat
     2701 tcaacgacga cactcactgt tgtattccag ccgaaaatct gctctactgc gagctgtcct
     2761 cctgtcagaa acagcatagc taccagaaag cttccgataa atagctgacc atgacgataa
     2821 gtagaaaaga cacgataact gatatttgcg acaataaacc ctaaaaaagt aactggaccc
     2881 acaagagccg ttgatgtccc tgtcaaaaga ctgacacaac ataaaatcaa aaattcaaaa
     2941 cgatctgtgt ctatcccgag gtttactgct tgttcatttc cgagatggaa aacgtctagc
     3001 acatgtcgat atcgaaataa caggtaccag cagccgataa tgatcaaaga agcaggcaaa
     3061 agatactgac tctggatatt gccaaagctt gcaaataatc tgccttgaag caaatcatat
     3121 tcattcggat ccatgatgac ttgtaaaaag gtactgacac taccaaaaag cgtacctaaa
     3181 atcatccctg acatcagtaa taaaaaaaga ttcgctctca tttttttcaa aagaaagaaa
     3241 aataacaata gactcagccc gaccatcagc ccaacgttca ataagaaaaa aactgtacct
     3301 gtctgctgaa gcatcgtcac tcctccgata aagaagaaaa ggaccgtttg gacaaggaca
     3361 tataacgact caagtcccaa tactgaagga gtcaaaaact ggttatgcgt aattgtctga
     3421 aagctgattg tggcactgct gacagctagc gagacaagga taaatgcaaa aattttctta
     3481 ctcctcaatg cacatgcaaa atgccagttt ccatatgtat ggatcgtcaa gtacattcct
     3541 gtaacaaata aaacaagaac aagcatacca ataaaaatag atggcttttt tttaagcata
     3601 tcgtcgtccc cttatcagaa gataaaggaa gatagcccca ccgatgattc caacgatcac
     3661 actgactggt atttcataag gaaaaatcaa cacgcgagcg acaagatcgc aaaatactaa
     3721 gaacaaacta cctgctaaag cagttacgcc aattgttttg tgcacttgat ctccatatat
     3781 tctagagaca agattcggaa tgatcactcc gataaaagga atgcttccta ccatgatcaa
     3841 catgactgca ctagccaatg atactaagcc aatgcctaaa aactgcatct tctggtaatc
     3901 tattcccaga tttactgcaa acgattcgcc cagaccaatg atcgtaaatc ggtaagcaaa
     3961 tagccaaacc aagagcaaca atggaacaac agcatacaat aactcataat ttcctcgtat
     4021 cacagtcgca aaattccctt gaagccatga agtcatattt tgtaccaact ggaattgata
     4081 agcaaaaaaa gtagcaactg aaccaatgat attccctaac atgactccaa tcaacggaag
     4141 catcaattga tttttctgga ggaacaatcg cgtaagggat aaaaaaagca atgttcctgc
     4201 aaaagcaaag caaaatgctg tcaaggatcg gacaatcaag gatgcaccag gtaggaaaat
     4261 catagccact aaaataccta aacgcgcact atccatcgtg cccgcagtcg ttggtgaaac
     4321 aaatttattt tgggtcaaat gttgcatgat caacccacaa atactactgg tcgctcccac
     4381 aaggatcaaa caaatcgttc gtggcaaacg agtcactagc aagatttgta agctttgccc
     4441 atctaactgg agcagttctg acaattgtac attcgatact ccgatgaaca atgaaatact
     4501 acttaaaata actaataatg ctactagtcg tccgtagcgc atccaatcca cctttctaat
     4561 tgagaatgat tttcattctt attttaagtg aatcgttgaa caaaagcaac cgctttttca
     4621 tatgttttag gtattattgg gaatatgcta taatcgtaag aaagacaaag agaagaagga
     4681 gggtattttt tatggcacaa gatgatgtaa aaagccattt ggataaggga ctttatggta
     4741 cacctttagt gaaccctgaa gaacagcata aatatttagg gacattcagg gaacgctgct
     4801 atcttagtat gactgttgct gaaatgatcc aaccaaaaaa caaagaaaac tttttaaaag
     4861 aactcaaaaa acatccagat gcttccatac tcttgaatgg ggctctaccc ctttctgtcc
     4921 aaaaccaata tatccagcta gcaacgaaaa acaatagccg tttcactgta gtcaacgatt
     4981 acgtcactgc aaatcctgag gaattcggat tgctcttaac agcaaaagaa gcagtaaatg
     5041 aacctgtgat tgatatcgaa aaaaaatacc cagaagaaaa ctcttcgcca gctgaaaaag
     5101 aaaaaccaaa aaagaaaaaa agcttctggg atcgtttatt ttaaaacgaa aaaacacata
     5161 aatttttaca tgcaaaaaaa gcttttgatc agattgacat caaataggac aatctgtcaa
     5221 aagctttttc ttttaaaata cttcaggata taacaatttc aatacattca tcgtcaatct
     5281 tctgcctttt cctgcaaaag gataaacatg catatgcatg gctggattga aatttgcttc
     5341 gatgatccca tatgcttttt tatcagttgt aggatcgatt tctttatctg gtataatcaa
     5401 atcgatacca ctaatcttag ctcctagtgc ttctaccgca gaaacggcga tttttttgta
     5461 agcttcagaa aattcatcag tcatatcgat cgaatcacca cctgtactga tatttgagtt
     5521 ctcccttaga taaacaattt gattggcttg gggaactgat tcgatcgtca acccttgttc
     5581 tttcaacatc aattgttcta attcacctaa ttggatcaat tctaaaggtg cacgatgatt
     5641 cgttccacgc aaagggtcac tatttttttc ttcaacaagt tctttcactg ttcggatact
     5701 gtcaccaata acattagcag ggactcgcaa catgatggct tggacttctc cgtcgatcac
     5761 aaagaaacga tattccgttc ccggcataaa ttcttctact aaaaccgaac tgtcttcacg
     5821 aaaagcaatg gctaaacctg cttgataatc ttccagacta gctccttcct tgaaaatcgt
     5881 gatacctaac ccgtaattgg ttgatttagg tttgatcaca aatgcctgct cggcaaatct
     5941 cggataagct tttaccgctt cttccatgga tgaaaattct gcgcctcctg gtacccggaa
     6001 cccagcttct tttaagactt ttttcgttac tgttttgttt tccatgataa gtgggacgat
     6061 atagctgtcc ttacttgtca tgtttgcatt tttcacatat tctacatggt cttggaattg
     6121 cagacgcaaa aattgatccg actcatctaa tactttgact tggacacctt tttggatggc
     6181 atcgaacata aatatctgag tagaaagttc catttcgcga aaaccggcca actgataggg
     6241 acgttcatgg ctttctttat aatattgatg tcccaatgcc acagcaactt cttgctgact
     6301 cgatagctgt gcttcttgat agagtttcgc tgctaacgtt tgttcaggat gatccatttg
     6361 agtcaatgct tcctctacta aataaagaga atccaaccaa tccaattcaa ccagcatttg
     6421 cttcattcct tcaagaattt ctctaccttc ttgatgaaat tctgtttgat ctgatggttg
     6481 ttctagtgct actttgttat ttcgagtcgt accttctgct acccattggt cacagtcttc
     6541 tttttcttct gtccataaca tgtacatcag gaataattgt aagaaccgaa cttgctctgg
     6601 attgataccc aatcttgcat aaggattcaa atcgatattc cgcaattcaa tataacgaac
     6661 acctgttgtt gctaaatctg cgactttctt tccgcctcgg aaacgaagcg gtgtataaaa
     6721 ttctttctcc tccgaaagtt tcccttcttc gatcattcgc tcgatatctg ctaaatactg
     6781 tttcatagaa gcataggaaa ctttcacatt tggatgattc gtatagccca gcgcactgtt
     6841 gcgaatactg cgaacaggtt cttgcggatg tgattcatct aaaaagtagt tcttctcact
     6901 cattggtgaa gcaccgaata aataagtgat catccagcga tagcgcataa agtttctcgc
     6961 tgttttcaaa taaagttctg ttttgaattc ggaaaaatct tgaaattctt cctgatggct
     7021 gaacaatgtt cttagcaact catcgccaaa ttcaaaattg aaatgaatac cgcttaccat
     7081 ctgttttcgc ttcccgtatt cttttgctaa ataacgtcga tacaagacat cttcgaaatt
     7141 ttttaatttt gcaataatga tctcttcgtc tttttccggt aaggcaggtg gcatacttaa
     7201 tggccagatc atctcttctt tcggtataga acgagcagtc acatcataaa cagcagcgag
     7261 atactgaaat aattcgggaa tagaatcggt aacaggggtg atcatctctg tttgtgtttc
     7321 gctaaaatct gtttggatat aggggtgata ggatcgatcg ccaaagattg ctggatgatc
     7381 ggtttttgct agatttcctg caagatcaac ccgctgtcct tcccgttcta taccaaatcg
     7441 agcttggtcg ataaaagggc gcgcattgac atgcaataat aattgcttaa aattcatcat
     7501 taagtgacct ccaattcata tgaatctaat tataatcaaa aagtctaaga aaaagaacga
     7561 aattgtttga gaaccgctta caaataagta taaaaatgaa ggcatcaaaa acacagctat
     7621 gttaaaaaca atcattcaaa caaggagctg caacttttgt cacagctcct gctttttaga
     7681 catgggttat tcttctttta gtaatccttt gaacaatcca acgacaaaat catttggatc
     7741 aaacggctcc aagtcatcga ttccttcacc aagaccgacc aatttcactg gtaaatgcaa
     7801 ttcattacgg atagcaagta cgataccgcc ctttgctgta ccatctaatt ttgtcaaaac
     7861 aagaccggtc acatctgttg tttctttgaa ttgttttgct tgaaccatcg cgttttgacc
     7921 agtggttgca tctaatacaa gaagtgtttc ttggggtgct tctggtaatt cccgctggat
     7981 tactcgtttt attttttcta attcgttcat caagttcact ttattttgca gtcggcctgc
     8041 agtatcaact agtaaaacat ctgcctgctc tgtctttgct ctttccaaag catcgaatac
     8101 gacagctgct ggatcgcctc ctgcattgcc acgaacaact tcaacaccag cgcgttcgcc
     8161 ccagacaact aactggtcga ttgctccagc gcggaaggta tctgctgctg ccatcaatac
     8221 ttttttacct tcgtttttga actgatgggc aagtttacca atacttgttg ttttccctac
     8281 accattaaca ccgacaaaca aaataactgt tagtccgtct tcttggatat ttagttcatt
     8341 tacttcattg attccttccg cttcatagag atccacaagt ttctcgatga tcgcattttg
     8401 gacttctgcc gattttttcg catttctgag tttcacttct tgacgtaatg cttcggtaat
     8461 tcgtatcgct gtttcaaaac ctacatctgc accgataagt gtttcttcta attcttcaaa
     8521 aaagtcttca tcgactgttc gaaaattggc aaacaactca ttcatccgtt caccaaatgt
     8581 tttacgcgtc ttttctaaac ccttgtcgta cttctcctgt acttcaactt cttctgtttt
     8641 ctcttcgcca gtaaaagcac gcttgatttt atcaaaaaat cccatttgac tccccctact
     8701 ctaatacaaa tttttggatc actttggcta cgccattttc ttcattgctg gctgtcacga
     8761 aatctgctgc ttctttgacg aaaggcacag cattctccat cgctacgcct agtccagcat
     8821 attggatcat cggtaaatca ttttcctcat caccgatcgc catgatttct tcttgtttca
     8881 aaccaagatc ttttgccaat aaagaaatac cataagcttt tgtaacacct tttggcataa
     8941 attccaaaag catacttctt gttttgatga tctcatacat ttcatggaat tctgctggta
     9001 tttctttgat tttaggatct aattcttctt ggtcataagc gattactgcc ttattaagca
     9061 caaattcatc tgtaatatcg cttatatccg ctggttggaa ttgcagcagc ttattcaaca
     9121 cattgtacaa ggattgcttc cttggggcac taggcagctg taaaaccaca ttgtcagaca
     9181 aaacatctag tggcagatcc aattgctgag ccagtttgta taagcgttgg atatctgata
     9241 cttccatcac tttcttttcc atgatttcac cagtatcatt tttttgtacc aaaccgccgt
     9301 tgaaagtgat gctgtagtca ccttcatcta ccagtcctaa ttcttgcaga taatgcgcca
     9361 tcgcagctaa ggggcgtcct gtacatagaa cgatcttgac cccttgctgt tttgcttgtg
     9421 ctaatgcttg tttattctca tctgaaatca gcttttgttc atttaataat gtaccgtcca
     9481 aatcgattgc tactaatttg atcattttcg ttcctacact ttcgtttcgt tgattttcgc
     9541 ctcttcattg atatcttcca tccgtacaga aacgattttt gatacaccgg attcctgcat
     9601 cgtcactccg tacagtacat ctgctgcgac catcgttcct ttgcggtgtg tgactacgat
     9661 aaattgtgtg tcatcttgga aatcgctcaa gtaacgacca aaacgtttca cgttggcttc
     9721 atctaacgct gcttctacct catctaagat acaaaacgga acaggacaga cacggataat
     9781 ggaaaagagc aacgcgatgg ctgtaagtgc tcgttctcct ccagaaagca aacttagact
     9841 ttgcagtttt ttgccaggag gttgtacttc aatctcaata cctgttttca gcaaatcagt
     9901 tggttccgtc agtactaatt cagctttacc gccgccaaac atatttggaa agacaatttt
     9961 gaattcttga cggatagctt caaatacttc tttgaatctt gtccgcactt catcatccat
    10021 ctcatccatc gtttcaaaca attggttttt cgctgctaac agatcatctc gctgtgttgc
    10081 taagaatgta tgtctttcac tgacttgttc gtattgttcg attgaattta agtttactgg
    10141 tcccagtttt tcaatctgtt cttttaagga tgaaaccttt gttctactgt cttcgatatc
    10201 ggttgtttct tgatagtctt gggaagcttt ctcaaatgtc agctgatatt ctgactgcag
    10261 ataggtcaaa tgattatcta aaatcatttc agctcggtct ttttggactt ctaatttgga
    10321 ttgttccgac agtcgtgctt tttgctcacg attacgctct gccaagactg cttcaagctt
    10381 atcgatctcc ttttgccttc tatctcgctg ctctttggct tttgccaact cggcttttaa
    10441 tgtttctcgc tgagcagata attcgttaat ttgtttttcc aaactttctt ctgtgacttc
    10501 atgatcagaa aagtctgctg tcaatgtcgc caactgcttt tctatcgctt cttgacgttc
    10561 ggttgcctct gctttttgga ctcgggcacc gcggagctgg atctgcagat gattaaactg
    10621 ctctttcaag acagcaagat ctgcttgttc ttgtgctttt tgactttgta cttgtgctct
    10681 tctggcttcc atctggtcgc tttcttgact taacgatttg atttcttcgt cgattttttg
    10741 tcgttgcgat tcaagatctg tttgtttgtc tttcaattcg ttcgtttgct tttcgtaatc
    10801 ttcgataaac tgctgcagtt cacgtgtctc aaaagttgag atttgctttt ctttttcaaa
    10861 acgttcaagt tcatttatga tgttttgtag ctgatttgtc gcttcttgtt cttcaaaacg
    10921 taattgttcg cctcgtgtac gcaaaacttc ttgttcttcg gctaaacgag ctgtttcttg
    10981 ttgcagctcc tgaacttttt tttcttgtgc ttggagttgt ttatctgctt cttcaaattc
    11041 actagtcaac tgtttcaatt cttgattttg tacaaacaaa ctgccttggt ttcctcgttt
    11101 attcgctcca cctgtcatgg atcctccggc attcatcaca tcaccttcta aggaaaccac
    11161 tcgatattga tagcggatcg tttgcgcgat tgcattggca ctagttaagt ctttggctaa
    11221 aaggatcgtt cctagcaggt tttgaaccac agtttggatt tgatcgggat aactgacttg
    11281 ttcactggca atccctataa acccttctac agctgctgct tgtgtcaata tatgagccgg
    11341 taattgtctt ggtttgattg tcgtcagtgg aaggaatgtt gctcttcctc ctctttgctg
    11401 tttcaaatac gtgatggctt gccgagcatc tttttcattt tcaacgatga catgctgggc
    11461 tgccccacct aaagctgttt cgatagctaa cgtgaaatct gcaggaacat cgatcagttc
    11521 agccactgct ccgacaatac ccgaaagctg ctgtttatgc tgcaagacca atcgaacccc
    11581 ttggtaaaaa ccaaaatagt tttcttggat ttcttgaagg cttttcttac gagcacgtag
    11641 ctgctggact tgattcatta attgatacat tttaggctgt tcttttgcta atgcttcttg
    11701 gaactttttc ccgttttgtt ggatcatttc taacttctct tttgtttcag ttaaagatga
    11761 ttttagctgc gcctgctttt gtgtcaattc ttctttttgt aagatcaatc ggtctactga
    11821 tgcttctact tccgattgct tggctaacgt ctgcttgctt tttgctgttt cttgtatata
    11881 ttgacgttct agatacttca gttcgtttcc tacagctgct tgttcttgca tcaaatcaac
    11941 atattgatct cttaattctg ccaataattc tttcgttgat ttttggtatt tttctaattc
    12001 ttgctgtgtt tttatgagat ttgcttcagc tttttggatc tctgtttctt tttctgctgc
    12061 tgctttcatc aagctttctt gaagcttttc aaaatgttta accttttttt gtgcttctgc
    12121 taatgaagtt tgatattctt gcgagctttt ttgtgtatgc tttgtccgct cctgcaaaac
    12181 atctttttgt ccttctgtct gcttcagttt ttccgaaaga tctaataata cttgctgatt
    12241 tttttcgatc agacgatcgg cttgcgcatt ttctttacgc tgcttcgcca aaatggattc
    12301 ttgttcttgg atcgactcag atagtttgcc taattctaga ttgaatttcg caagctgtgc
    12361 ttgtttgttg tcccaatctt tttttgctgt cttgatttca gcaaccatca aagagacatc
    12421 ggtttgagtc agtgtttctt ttagccgtaa aaactccttt gctgcctcac tctgggcggc
    12481 caaaggagtt aactgttctt ccaattcgtg gatgatgtct tggacgcggc tgaggttgtc
    12541 ttctgtttca aaaagcttct gttctgcttt ctttttccgc tgcttgtatt tcaagacacc
    12601 agctgcttct tcaaagatcc cacgacggtc ttccggttta ctactgaaaa tcgcttctac
    12661 ttttccttga gaaatgattg agaacgattc ttttcctaat ccagaatcta agaacaattc
    12721 ttggatatct tttaatctgc agctttgttt attgatgaaa aactcgcttt ccccagtccg
    12781 tcgataacgg cgggttacac tgatctcatt gtactccaat ggcagataat gatcactatt
    12841 gtctaaaatc actgtgactt ctgcaatatt cagctgctta cgggtatcgg aaccggcaaa
    12901 gatgatatcc ggcatttttc cgcctcgcag acttttagca gattgttctc ctaataccca
    12961 gcggatagcc tctgtaatat tgctttttcc gctgccattt ggtccaacca ctgctgtgac
    13021 gctattttca aaatcaatga ctgtcttgtc tgcaaatgat ttaaaaccgg ctatttcaat
    13081 tcgtttcaaa tacaaagctt tccctcactt acttttacag tgctgccaat gcattttccg
    13141 ccgcagcttg ttctgctaat tttttcgatt tcccttgtcc agtgccaatc aattgatcat
    13201 ctacataaac ttcgatccaa aatactcgtt catgagctgg accttcttca ttgatcaatc
    13261 ggtattcgat tgatacatca cctgattttt gcaagacttc ttgtagtttt gttttatgat
    13321 ccatctcatg tgaaaaagca cctgctttga ctttaggaaa aatcactttt tcgatgaatg
    13381 catgggtcgt gtcgaatccc tggtcaagat ataacgcgcc taaaaaagct tcgaataggt
    13441 cgcaaagcaa tgctggtcgc gtacgtcctc ctgaattctc ttctcccttg cccaacaaaa
    13501 catattgatc aaagtgacat tctttggcaa atttcgacaa gctgtcttcg cgaacgatcg
    13561 cagctcgtgt ttttgtcaat tttccttctg gcaaatgagg atataaacgg aataaatact
    13621 gagaaaccat cagttctaaa acggcatctc ctaaaaattc aagtcgctca ttatccgata
    13681 attgcagatt gcgatgctca ttcacatagg atgaatgggt aaaagcttgt tcaagcaaat
    13741 ttagatcgtg aaaaacgatg ttatagtttt cttttagttc ttttgtcagc tggttgtcca
    13801 ttttccacat tccttttatg gatatttttt cgatacttcc ttattatact ggttttttgt
    13861 caaatttgaa atggttcggt caaaaggtaa gtaaagcttt aataatccta gtgattgacg
    13921 agaagttgtg aaaaattcgt ttagtttcga gtatgaagaa aaaattttgt gaaaatggct
    13981 ttccatattt ttgcataaat ttgacttaat accgagaaac tggactttga acaccgttta
    14041 ttggagaagt catgaaatca gcgccttgac ctgaatatta gagaaataaa cagaaaaata
    14101 tcaaaaaacc gaaaaaggac atcgcccttt ttcggtcatt tttagtgatt atgaacgtga
    14161 atctgctgta acagaaacat ttgcccatgg gtttggtaca tcataagacc aatcccagtc
    14221 tttgacacgg tcgttgactg gcaatacttc attacggaac aacgttggaa tcgcaaatgc
    14281 ttcttcaaat gcatattgtt gccatttttg atatgcttct ttttggtact cttcatcaaa
    14341 tgctttgctt gaactgatat cgttgatcaa ttttgtattt tcttctgatt cataacgagt
    14401 gtagttgaac gcagcatttg gtccccatag tccacttgga tttggatcag ttcctgtatt
    14461 ccaagcagct tggtaaacat caatttcagg atcatcgttt tctaatttat catagaaaga
    14521 attgaattcg atcagacgac ctgttgtgta ttggacatcc aaaccaattt ctttccattg
    14581 ttggatatag taatctgcca atggttgcgc tgtttcccca ccggacatcg aagcaaagtt
    14641 gattgttagt ttatttccat ctttgtcttc tcttaaacca tcgtcatcca catctttgta
    14701 gccagcatca tctaatagtt ttttcgcttt gtctagatct tgtgtgaatc ctttcatctc
    14761 atcatcatgg tatgactgga atactggagg aatcaatgaa gtcgcattgc ttctcaaacc
    14821 tgcatagaaa cgttcaccta ctgcattatt atctaatgca tagcccattg cttgacgtaa
    14881 tgatttatca gccatttttg aatctggatt gtattccaca ctctttgtat ctgcattcca
    14941 tttacctaac ttgaatccta gataagtata agaagtttgt tcacggccta agtttgtata
    15001 gccagcaatt tctttatagg tatcgtaagt atctgttggc atttggaaaa tcatatcata
    15061 taatttagag ttcaatgctt cagttgcact tgccatcggt acagatttca tcgtgatctt
    15121 tttcaattta ggcgtaccat tccagtagtg ctcgtttggc aagtattcta ctgattctcc
    15181 tgcaacgata ttactcatgt aataagggcc aaaagtcact ggatttttac gtacttgatc
    15241 gcttgattcc atatctttga ttgctacacc ttctaacgta tgttttggtg ctgcatatga
    15301 ccagatacca ccacccgatt gaagcatgct tgggtttact tctttatatt cgatttcaac
    15361 tgttttgtcg tctatttttt tgattccgga gattgtgtcg gcacttccat catgatattc
    15421 ttccatacct acgatgttac ggaaagtgtc gtcataacgg ataccagtgt aatctttatt
    15481 cccaatgatt tcgtaaggga aaatcacatc gtccgccgtt acatcttcac catctgacca
    15541 tttgacgttg tcttttactt tgattgtcgc ttttttcgta tcttcatcta gatcaagcgt
    15601 tgcagctccg tcgtctgtga ttctgaaatc gtcatcgttt gcaaatagcg gttcatgaga
    15661 aggagccata aaagctgcat catatgaatc ttggtaaaac tcccactgga acaatccttt
    15721 aaactgactg tccgttgcga ccgcagcttc taattcgccg tcttcgattg catcatcatt
    15781 attttttgtt gcaattggaa aagttaattc ctctttgtta ttgccgttgc ctccagagtt
    15841 gtttccagag gtatctgtac ctccgccccc gcatgctgca agaacgacag cagacatcag
    15901 cgctgcaaat ccaaaaatcg ttttcttcat tcctatttcc cccttcgctt ctatactagc
    15961 ctaaccgttg gcgcgcatct gcagaacgct tgaatgcttg tccaacataa ttgatactca
    16021 acatcaaaac taaaataagg attgatgcgg gtaaccagat ccattgccga ttcactaaga
    16081 cttctccgtt acttgcataa ccgatcaatg tacctaaact aggtacattt gccggcaagc
    16141 cgaaacctaa gaaagtcaaa gttgtctcga taccgatgtt tgccgcaaag ttcatcgtca
    16201 aattagtaat gattaatgaa cttagattcg gcatgatttc gcggaacatg attttaaaat
    16261 cattggttcc cattgttttt gaggcactga cataatctct tcttgcttca gataatgtct
    16321 tgcttcggaa taatcgggct ttagctaccc aataaaatgc actcatgatg aatataaagg
    16381 accagacatt gtatctggaa ataatcgata cgaagacgat aatgatcagc atgatcggta
    16441 gaatcatgat aaaatcaacg atccgcatca agatgttgtc aatgatccct ccgtagtagc
    16501 cagagatgat ccctaatcca accccgatga tcgaagtaag gatcgtgatc gcaaatccga
    16561 tcgtaacaga gtttctagca cctatgatca attgtcctaa tacatcacgt ccgccttcat
    16621 ctgctcccag aatgaacttt tgtccttgtg tcgaaactgt tcctggtgca gcgtatttgt
    16681 cgaagatact aacatacatc actttgtctt gatcggtcag catcgcaccg ataaagatca
    16741 ctaataataa aaccactagc aaacctaatg aaaaaatagc aattttatct tttttaaatt
    16801 ctcgagcaat cattcgtatc cccataggag gaatactttc ttgaactgct gtattgtttt
    16861 tttcactcat cgatccattc tcctttctga ttggttttat tgaatccgaa tacgcgggtc
    16921 aacgatactc atgatgatat ctgaaagtaa tgtccccact agcgtcgcga ttcctaaaat
    16981 caatactaag gatgtgatca cggtatagtc ccgttggccg atggaatcaa taaatagttt
    17041 tccgactccc ggataaccaa aaatcttttc gataactaca gaaccagcaa tcaacgaagt
    17101 gatttcatac cccatttgag aagccatcgg caacgctgca tttcggaaaa tatgtcgact
    17161 gaatacttta ttcgtcggta cccctttgga acgggctgtt ctgacaaaat cgagcgattt
    17221 cgaatcgatt acttcacttc gcagatattg gattgtgacg gcagtcgcca aaaatgcttg
    17281 ggtaattgat ggtaacagaa tatatttcaa acggtctatc cattgagtga taaatccacc
    17341 ttgcaaacct agtgtttgtg atccacttgt tgggaaccag cctaaacgat aaccaaaaac
    17401 aaatagcatg atcaagccga agataaataa aggaacagca aaactaaaga agttgtagat
    17461 tacgacaatc ttatccaaga ttgaattttg ataccggcca gcaagcatcc ccagaggcac
    17521 agcaatcaaa taagtaagga caactgtcaa gatcgataga tataaagtgt tgatgatccg
    17581 ttcgccgatc aagtcagcaa cagagcgttt gtataagaaa ctttgaccaa aatcgccttg
    17641 caaagcattt ccgatccaac ggaagtactg gacataccat gggtcattca agccagctgc
    17701 ttgtcgcatc tgttcgatca ctgccggatc ctgatttgga ttgatcaaac cggtaaatgg
    17761 atcccccggc atcgcttggg ctaataagaa gatcagaaca cttaaaataa taacttgcgg
    17821 gatcatcaat aaaacacgtc gcagaatcgt tttccacatt agcttgcacc tccatctttc
    17881 aacgccactt tgtgtgtagt tgtgatcgta cgcaaatcgt agactcgacc agtcgcatca
    17941 tagtattctt tttggttttg gatatactct tgttctactc ttcgtcgatt ttctttgtgg
    18001 ctttccctat tttctacatc gatttgcgga atagctgaaa gcagacgttt cgtatagata
    18061 tgtcgcgggt cattataaat gtcttctctt gtaccgattt ctacaaatcg acctttgtac
    18121 ataattgcga tattgtcgca catgtgtttt actacaccta aatcatgtga aatgaataaa
    18181 tagctgaggc cgtattcttg ctggattcgt ttcatgaaat tcaatacttg cgcttgaaca
    18241 gatagatcca gagcagatac aggttcatcg gctacgatca gttttggatt ggtagcaaca
    18301 gcacgggcta ctcctagacg ttgacgttgt ccgcctgaaa attcatgagg atatttatat
    18361 agcgcatctt ccggcatccc aacgatatct agcagtcctt ttaccttttt cttttcttct
    18421 tgatcgctta accgttcaaa atttcggatg ggttcagcaa tgatatccaa cactcgcttt
    18481 ttaggattca aactggacat tgaatcttgg aagatcatct gcacatcctt gttataaccc
    18541 atttttttgc ggttgcttcg cttcgttaca tcttttcctt catacatgat ttgtccggct
    18601 gttacctttt ctaatcccac aacggctttt cccgtagtag attttccaga gccagactct
    18661 cccactaaac catatgtctt tcctttttcg ataataaaat ccacaccatc tacagcatat
    18721 acatgatccg ttactcgatt gaagaatcca ctgcgaatag ggtaatgaac tttcaggtct
    18781 ttgattgtga ttaattctgt catctacacc tctccttttt cgtctctaaa gtggaaatgc
    18841 ttatagcagg agcaacgtac caagtggttc ggtgcgactt catgaaggat aggctcttct
    18901 tcatgatcgc ttgctggtat ccatggaata cgtggtgcaa accgacaacc tttacgaggc
    18961 atgtttttca gagaaggaac gacaccttcg atcacatgga gttctgcatc atgatttcct
    19021 tcttgtggta tagagttcag taaagagcgt gtataaggat gcttaggatg atcaaacagt
    19081 tcttttacac ctgctacttc aacgaactgt ccgccataca tcactgccac tcgatcagcc
    19141 atttctgcta cgactcctaa gtcatgagtg atcaagatga tccctgtttt tgtttcttcc
    19201 tgaaggtctt tcagtaaatc aagaatctgt gcttggatgg ttacatctag ggctgtcgtc
    19261 ggttcatctg caatgacgat tggaggtttg caggcgattg cgattgcgat gatgacacgt
    19321 tgtctcatac cgcctgatag ttcatgggga tattggcgtc caactctcgc aggattagga
    19381 atccctactt gttctagcaa ttctagtgca cgagcttgac gctgttctgc attcatcttt
    19441 gtgtgatagg aaagtccttc tttgatctgg tcttcgattc tcatgagagg atttaatgca
    19501 gacaacggat cttggaaaat cattccgata tcatttccgc ggattttatt atagagcgtt
    19561 tcgttcaggt cgattaaatt cagatcgtta tataaaatct cacctgtaat ttttgtgtta
    19621 ttaggatcaa gtaatcccat aatcgtagta gccaaggtac ttttcccgca cccagattcc
    19681 ccaacgattg ctaaaatctc gtttttgtct aaagtaaatg aaacgtcatc tactgcatcg
    19741 taatagtcat ctttgagtcg gaatcctgta tgtaggttct gaacttctaa taattggtta
    19801 tcggacaatc gaatacccct ctctgcttcc taaatggaag tttttcttaa taaaaaagga
    19861 cattacacag aataaatcgt tttataacaa aggttattct tatctttgtg taatgaatta
    19921 ttaataatta tatagaaaac cgctttactt tgcaacataa tttctgttta tcgccagaat
    19981 tatcagaaaa atgactttta cgtgtcaaaa agattaaaaa aagaaggata aaaagctgca
    20041 tgcagctttt tatccttctt tgcttttatt tgaaattatt taagatggct gctgatataa
    20101 tctactgcgc caccgactgt ttcaatttgt tcagcgtctt catctgaaat ttccgtaccg
    20161 aattcatctt caagctctaa aacgaattcc ataatactaa ttgaatcagc ttttaaatca
    20221 tctttgatgt tcaattcatc cgtcacttta tcggtatcta tctcaaaatg atttgaaata
    20281 attttcgcta ctttgttaaa tacttcttcg cgagtcaact ttttcacctc caagaatcat
    20341 acacttgtca attacctaca ccatggctaa ttttactggc atttttcagg aatgtccacc
    20401 tttttttcaa tggaacggat cgcttcagat atccaaatac tattcttcgc ttttttcaaa
    20461 ttgttcgact aattgtccaa ctacatctgt ctcaagcatt gtgtggattt ggcggatcgt
    20521 atagcgaaca gcatcggggc ctgttgctcc atgagtttta atgactggtg ctttcaagcc
    20581 aaacaaaacg gcaccgccat gtttggaata atccatttct gctttcaaag aacgtaagcc
    20641 gtcttttaaa agcatagcgc ccatttttcc tttgatccct tcatttagaa tagcagattt
    20701 taatagattc atcatattca ttgctgttcc ttcaatagat ttcaaaacag cgttgcctgt
    20761 aaatccatct gtgaccacta catcagccac accattgagt agctcacgag cttctacgtt
    20821 tccaataaaa ttcaagcttg tttcattctg aaggagttca taggcttttt tcgtcacttc
    20881 actacctttt gttgcttctg ttccgttgtt taagagtgcg acacgaggac gtccgatacc
    20941 tcggacttta cgtgcataaa aagaacctaa aataccgtat tgcaacaaat gttctggttt
    21001 gttttctgcg ttagcgccaa gatccagcat atcgaaaccg gcaccttctt tccctactac
    21061 tggcaaggta gacatcaagc cagggcgttc gatatttttg atacgtccta cgatgaacaa
    21121 ccctgctgct agtagcgcgc ctgtattccc tgctgaaaat acagcatctg cttcaccgtt
    21181 tttcactgct tgtgcagcaa ggaccattga tgcagtcttt tttcgtcgaa ttgcttttac
    21241 tggctcatca tcactattga ttttttcgtc tgtatggata atcgtgatgt ttttttcatt
    21301 tgttaagtat ttcttgattt ctgcttcttt tccgtaaagt aagaattcta cttctggaaa
    21361 atcttgctgc gctaaagcca cgccttctac aattgcttga ggtgcgtgat ctccgcccat
    21421 tgcatctacg gcaattttca ttgtttccac tcctttttcc gtcatcagta taaagtatag
    21481 cacagtctgc taatcgaaat aacgattatc ctgattttct atttggaggc tttgcgctaa
    21541 aggctgatac tcaggaagca tctgccaatc ctttatttgc caaagggctg cagcctcttc
    21601 tctggctacc tccaaaacat ttccatccgt caccaagtca gcaatcgcaa attgaggcag
    21661 accagattga cggaagccaa atacttcacc aggaccacgt aattccagat ctttttcgct
    21721 tagtacaaaa ccattggtgg tctctgtcat gattttcatc cgttccacac ctagttcatt
    21781 tttaggatta gcaactaaga tacagtagga agcatcactt ccgcgtccaa cacgtccacg
    21841 aagttggtgc agctgtgcca atccaaatcg atccgcatcc atgatcagca tgacggtcgc
    21901 gttcggcacg tttaccccta cttcaattac agtagtcgaa actaatatct gcatctggtt
    21961 gtctttgaag gcttccatga ttacttcttt ttcctgattc ttcattttac catgtaacaa
    22021 gccaacttca tattttggtg caaatagttc acgaagtttt tcatagattt ccactgcatt
    22081 ttttacatct aatgcttctg attcttcaat cagcggacag atgacataca tttgatgtcc
    22141 tcttgccagt tcttttttcg tccaatccag tacactgtcc aattgaggtg ttcttaccca
    22201 gcgtgtttcg atcgggatac gtccagctgg aagttcatca atgatagata catccatctc
    22261 accatatgct gtaatagcta atgttctagg aatcggagtc gctgtcataa ataagacatc
    22321 tggtcgccag cctttttcac gaagtacttt ccgctggttc acgccaaaac ggtgctgctc
    22381 atccgtaata actagaccta aattctgaaa ttcgactcca tcttggatca atgcatgtgt
    22441 gccgatcacg acgttaattt ccccttgctc tagtttttca aggagctcac gtcgttcttt
    22501 tgctttcgtt gaccccgtaa tcaatgcgat ggttacttca ttgggatcaa acagctgttg
    22561 caagctttcc atatgttgtt gtgccaagat ttctgtaggc accatcaacg caccttggaa
    22621 acctgctgta acggtagcgt ataacgcaat cgcggcaacc actgtttttc cgctacctac
    22681 atctccctgc aaaagtcgct gcatatgctg cggactgcga agatcccgac agatttcatt
    22741 cgttacacgt ttttgtgcat ttgtcagttc aaaaggtagc ttttgagtaa attgtttcaa
    22801 tttttggaca tcataaagga tctccaaacc attcgtttct gctttttctg cttttttcaa
    22861 tccttgaaga cgcatttgaa ataaaaagaa ctcttcaaaa acgacacgtc gtttggcttg
    22921 atggttttct tcgtggtctt ttggaaaatg catacttctt acagcagaag ctcggtcaag
    22981 cagtcgatat ttctccacaa ggttgtttgg cagattttct tcaatcaatg tgccgtattc
    23041 ttgaaaccct tttttgatta gatcaactaa tgtcgtttga cgaattgatt tgttgacgtg
    23101 atatatcggc gaaaagtcat ccattgtttg agaacctaaa atcttcatcc cgttcaaggc
    23161 tttacgcttt gcatcccatt taccgtatac ggcgatttcc tccgataaaa tgactttttc
    23221 ttttagataa ggttgattaa aaaaagaaac gttgaacaca tcatgttctt gcatcatacg
    23281 aaattgcaat cggctttttt tataaccaaa tcgattcatc acaggaggcg agactacgat
    23341 tcctttcaaa gtaacctttt cttgatcctg gatctcgttt agatttcttt cctgaatatc
    23401 ttcgtagcga aaaggataat aagtcagtaa gtcttcaatc gtttggatgc ccaagctggc
    23461 aagactagct gctctttttt ctccgactcc tgccagcata gtgattggat catctaaaga
    23521 acgcataaat ttttcctccc tcctttaaca aaaaaaggac ttggtcagcg atcatacctg
    23581 atcactaacc gaaggccttt tatgaatgaa tactgtgtta gaaagatatt ctgacacaga
    23641 aattattcag cagaaaatag ataaggatag acaggctgat ctccttggtg gatctcgatt
    23701 tccaaatcag gatgtttttc tgttagtttc tcagcaaatt catttgcttg ttcaactgtt
    23761 ccttcatcac ctgccaagat cgttacgatc tctgtgtcct cattgatcat ttgttctagt
    23821 gtacttaacg ctgttgctgt gatatctggg ttagagatca ggatttttcc atcgatcatg
    23881 cccaaaaagt ctccttcttt gatctctaca ccgtcaattg aggtatctct gatcgcgtga
    23941 gtgacttgac cgctcacaac gctagacaac atttctgtca tcgtttcttt gttttcttct
    24001 aaagaagctt gatcattgaa tgaaagcata gctgtcaatc cttgggaaat cgttcttgaa
    24061 ggaacaactg ctactgggat atctgctact tctgctgctt gatcagctgc catgaagatg
    24121 ttcttattgt ttggcaagac aatcacttta tctgcgttta cttcttgaat cgctttaaca
    24181 atgtcttccg tacttggatt cattgtttgt ccaccactga taacataatt agcgcctaaa
    24241 ctttcaaaca gctcttggac gcctttacca gcagcgatcg ctacgatacc atacgggata
    24301 cgtggtattt cttttttcgc aaactcgttc acttcttcat cgtgttccaa aatcgtctca
    24361 tgctgcaagc gcatgttgtc gactttgatt ttgactaatg aaccgaattt ttgtccataa
    24421 ttcaagactt ctcctgggtt ttctgtatgc acgtggactt tgatgatctc gtcatcattg
    24481 accacaagta aagaatctcc tataccatct aaatagttac ggaattcttc gtaatcgaat
    24541 gtactatcga ctgtaggtcc ttctccgatt tttaccatga tttctgtaca gtaaccaaaa
    24601 tggatatctt ctgtcgccat ttgaccttgg acactgcgat gatgttctgc attgaccata
    24661 tcatccattt ctgctggaga aggttcatat gtttcatccg cttggaattc tccattcaat
    24721 gcacttaaaa agccctcata aacaaacagc agtccttgcc ctccactgtc tacgacaccg
    24781 acttctttta ggactggaag caagtctgga gttttagcta aagcacgctt tgctcctttt
    24841 acaacagctg tcattacttc aacgacatca tcagtacttt ttgctttctt ttctccagct
    24901 ttcgctgctt ctctagctac tgtcaaaatc gttccttcta ctggtttcat tactgcttta
    24961 tacgcagttt caactccatg agtgaaggct gcagcaagat cagaagcatt taatgagaca
    25021 acatctggaa tttgcttaga aaatccacga aacaattgag acaaaataac gccggaattt
    25081 ccgcgcgcac ccatcagcaa tccttttgaa agcattgctg ctaattctcc tactttttct
    25141 gaggttgaat ctactactgc cttagctcca ctagtcatag atagattcat atttgttccc
    25201 gtatcgccat ctggcacagg gaatacattt aatgaattga cgtattctgc atttacttgc
    25261 agacgattcg cacctgcctg gaccatttcc tggaactggc ttgcacttat ttctgttaca
    25321 ctcacctaga taatcctcct tcagatcgac tgctgagtta ccagccagtc tcctgcctag
    25381 tctggcagta cacgaacacc ttgtacaaag acattaactg aattggcagt tacgcctaat
    25441 aatgcctcta gattgtattt cactttttct tggacgttgc gggaaacttc agagattttc
    25501 gtaccatagc tgataatcgt ataaacatct acagcaatac cattatcttc ttggcggaca
    25561 actactcctc ttgagtaatt ttctttacgc aaaatctcag taatattatc tttaatttga
    25621 tttttgctgg ccattcctac aacaccgaac acatctgtcg cggcgccacc aacaactgtt
    25681 gcaattacat cattggtaat ttcaatggtt ccagctgacg tttgaatttt tacagccatg
    25741 aatgagcctc cttaacgggt aaaaaactcc catttttctt ctatcatatt ttatcatagc
    25801 gcaacttgaa ataaaaggca gttacccaaa gtactcaaaa accttgcaaa atttcaggta
    25861 taaccttgtt taatttacaa ttattcttct caattgtcaa ttgtatctat gaaaatgtac
    25921 ttgcaaattc ccattctata tgatatgtta gtctggtatg agctgaatta ttctattgct
    25981 ccaaatcaag gcaaaggagg aaattaccat ggcaaaagtt tgttacttta caggtcgcaa
    26041 gacaaaaagt ggaaacaacc gttcacacgc tatgaactca acaaaacgta ctgttaaacc
    26101 taacttacaa aaagttcgcg ttatggtcga cggaaaacct aaaaaagttt gggtgtcaac
    26161 tcgtgctttg aaatcaggaa aagttgaacg cgtttaattg aatgatactt ggaccgttca
    26221 gatagtgaac ggttttttat ttaataaaaa tttttaaggg tgggactttt gattcctacc
    26281 ctttttttgc gtttattttt gttatacgaa caaattatga agttatttat tctaaatttt
    26341 atttttttgt gaaaactaga ctctttttaa gcagagaaaa acaaggatat gataaactga
    26401 atacaacttc atagaaattt ggtggaaaat aaatatgaaa aaaaatttaa agtcattctt
    26461 tagagaaaac ggacgctacg cagcagctag tttcctgatt ccgtttttga ttttagcact
    26521 tgtttatcta agtatcggaa
//
LOCUS       DACDMM010000020        26489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDMM010000020 DACDMM010000000
VERSION     DACDMM010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.020"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            104..973
                     /locus_tag="IWD16_000668"
     CDS             104..973
                     /locus_tag="IWD16_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP9087752.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1138..2409
                     /locus_tag="IWD16_000669"
     CDS             1138..2409
                     /locus_tag="IWD16_000669"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9087753.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2406..3701
                     /locus_tag="IWD16_000670"
     CDS             2406..3701
                     /locus_tag="IWD16_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP9087754.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3800..4063
                     /locus_tag="IWD16_000671"
     CDS             3800..4063
                     /locus_tag="IWD16_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP9087755.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4508..4957
                     /locus_tag="IWD16_000672"
     CDS             4508..4957
                     /locus_tag="IWD16_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9087756.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            5014..6432
                     /locus_tag="IWD16_000673"
     CDS             5014..6432
                     /locus_tag="IWD16_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9087757.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6432..7292
                     /locus_tag="IWD16_000674"
     CDS             6432..7292
                     /locus_tag="IWD16_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP9087758.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7466..8239
                     /locus_tag="IWD16_000675"
     CDS             7466..8239
                     /locus_tag="IWD16_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9087759.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8386..8751
                     /locus_tag="IWD16_000676"
     CDS             8386..8751
                     /locus_tag="IWD16_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP9087760.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8899..10107)
                     /locus_tag="IWD16_000677"
     CDS             complement(8899..10107)
                     /locus_tag="IWD16_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9087761.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10126..10404)
                     /locus_tag="IWD16_000678"
     CDS             complement(10126..10404)
                     /locus_tag="IWD16_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087762.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10597..11253
                     /locus_tag="IWD16_000679"
     CDS             10597..11253
                     /locus_tag="IWD16_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9087763.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(11695..12492)
                     /locus_tag="IWD16_000680"
     CDS             complement(11695..12492)
                     /locus_tag="IWD16_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP9087764.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12489..13673)
                     /locus_tag="IWD16_000681"
     CDS             complement(12489..13673)
                     /locus_tag="IWD16_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9087765.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13698..14945)
                     /locus_tag="IWD16_000682"
     CDS             complement(13698..14945)
                     /locus_tag="IWD16_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP9087766.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15160..16905
                     /gene="dnaX"
                     /locus_tag="IWD16_000683"
     CDS             15160..16905
                     /gene="dnaX"
                     /locus_tag="IWD16_000683"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP9087767.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16925..17239
                     /locus_tag="IWD16_000684"
     CDS             16925..17239
                     /locus_tag="IWD16_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP9087768.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17264..17860
                     /gene="recR"
                     /locus_tag="IWD16_000685"
     CDS             17264..17860
                     /gene="recR"
                     /locus_tag="IWD16_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP9087769.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17924..18568
                     /locus_tag="IWD16_000686"
     CDS             17924..18568
                     /locus_tag="IWD16_000686"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP9087770.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18583..18912
                     /locus_tag="IWD16_000687"
     CDS             18583..18912
                     /locus_tag="IWD16_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087771.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18926..19864
                     /gene="holB"
                     /locus_tag="IWD16_000688"
     CDS             18926..19864
                     /gene="holB"
                     /locus_tag="IWD16_000688"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP9087772.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19900..20724
                     /locus_tag="IWD16_000689"
     CDS             19900..20724
                     /locus_tag="IWD16_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP9087773.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20717..21064
                     /locus_tag="IWD16_000690"
     CDS             20717..21064
                     /locus_tag="IWD16_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP9087774.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21133..22005
                     /gene="rsmI"
                     /locus_tag="IWD16_000691"
     CDS             21133..22005
                     /gene="rsmI"
                     /locus_tag="IWD16_000691"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP9087775.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22114..23235
                     /gene="dinB"
                     /locus_tag="IWD16_000692"
     CDS             22114..23235
                     /gene="dinB"
                     /locus_tag="IWD16_000692"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP9087776.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23289..23891)
                     /gene="nrdG"
                     /locus_tag="IWD16_000693"
     CDS             complement(23289..23891)
                     /gene="nrdG"
                     /locus_tag="IWD16_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP9087777.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24206..26395)
                     /gene="nrdD"
                     /locus_tag="IWD16_000694"
     CDS             complement(24206..26395)
                     /gene="nrdD"
                     /locus_tag="IWD16_000694"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP9087778.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT         8488 a         4828 c         5573 g         7600 t
ORIGIN      
        1 ttgattttag tagaaaacta ccgaaatgtt tggtaaaata gggtcgttgg ttaagaattg
       61 tcttaacaaa tttagtttta caaaacttag gaggaatttt attatgccag tagtatcagg
      121 agctgaattt ttaaaagctg ctcgtaaagg cggttacgct gtcggcggat tcaacacaaa
      181 caacttggaa tggactcaag caatccttga agcggctgaa gcaaaacaag caccagtact
      241 tatccaaaca tcaatgggtg cagctaaata catgggtggt tacaaaatct gtaaagattt
      301 aatcactgat ttagttgaag caatgaacat cactgttcca gttgctatcc acttagacca
      361 tggtgattac gaagctgctt tggaatgtat cgaattaggc tatacttcag taatgttcga
      421 tggttcacat ctaccatttg aagaaaactt gaaattagct aaagacgttg ttgaaaaagc
      481 acatgcaaaa ggtatctcag ttgaatgtga agttggttca atcggcggag aagaagacgg
      541 aatcatcggt aacggcgaat tagctgatat tgaagaatgc aaacaaatgg ttgctacagg
      601 tatcgactac ttagcttgcg gtatcggtaa catccatggt caatatccag aaaactggaa
      661 aggattagct tttgaccact tacaagcaat tgctgatgct gtaggtccag acatgccttt
      721 agtattacat ggcggatcag gtattcctca agatcaaatc gaaaaagcta tctcaatggg
      781 tatctcaaaa gtaaatgtta acactgaatt ccaattatca tttgctgcag ctactcgtaa
      841 atatatcgaa gctggtaaag ataaagaagg taaaggattc gaccctcgta aattattagc
      901 tccaggtaaa gcagcaatcg ttgaagatgc taaatctcat atcgactggt tcggttcagc
      961 taacaaagcg taagcatcca cataacagat acgatatata ggaatggaga acgacttttg
     1021 aagtcgctct ccattctttt ttacttaaaa cggcagacca tgctcgtttc ttttttttat
     1081 ctatgataaa atacaagcat atccatgaaa aaagattaat aaacaaggtg gaaacagatg
     1141 aaaaaaatcg taatcaaagg aaaccgacct ttagctggtg aagtaacgat cagcggtgca
     1201 aaaaatagtg cagtcgcatt gattccagct gcaatcttag ctgattcccc agtgatcttg
     1261 gaaggtgtgc cggacataca agatgttcat tcattaattg aaattttaga gatcatgggc
     1321 gcaactgtcc atttttccaa caacatacta gaaatcgatc ctcgagaagt cgtatcgatt
     1381 ccgatgccgc acggaaaaat caatagtttg cgtgcttctt attattttat gggaacactg
     1441 cttggtaaat ttggcgaagc ggtagtaggc ctgccaggtg gctgttattt aggccctaga
     1501 ccgattgacc tacacgtcaa aggatttgaa gctctaggtg ctgaagtgac aaatgaacat
     1561 ggtgccatgt atttacggac agaaaataaa ggattgcgtg gcaatcggat tttcatggac
     1621 gtggtttcag tcggagcaac gatcaatgtg atgttagctg ctgtaaaagc aaaaggacag
     1681 acagtaattg aaaacgctgc acgagaaccg gaaatcatag atgtggcaac tttactgaat
     1741 aatatggggg caaaagttcg aggagcgggt acagatgtga tccgtatcga aggagtcgaa
     1801 acattacatg gctgtcggca tttcatgatt cctgaccgca tcgaagcagg aacctatcta
     1861 gcattagcag ctgcagtagg aaatggtatc aaagtgaaaa atgtgatctt tgaacatttg
     1921 gaaagcttta tagcaaaatt gcaggaaatt ggtgtccata tgaaaatcag cgaagatgaa
     1981 atcgaagttt acccctcgaa agaattaaaa cccgcaaaca tcatgaccta tccttatcca
     2041 ggatttgcca cagatctgca gcagccactt actgcgttac tattgatgac tacaggcact
     2101 tcagaaatca tcgatacgat ctatgcgaaa cgtgtgaacc atgtgccgga actagcacga
     2161 atgggcgctg atattaccgt ggaaggaaat atgatcatcg tgaatggtcc aaataaactt
     2221 cacggcacag aagtcgtggc ttctgatctt cgggcaggtg cttgtttagt gattgctgga
     2281 ctattggcag aagggacaac gacgatttac aatgtagatt atatcttacg cggttatgat
     2341 catattatcg agaaactaac agccttaggg gcatcaattg aaatgataga ggaagtggga
     2401 gctgaatgag cgattatttg acaatggcag aactagagaa caaaacgtta aaagaagttt
     2461 atagttatgc caaagaattt aaaattcctt attacagtaa gatgaataaa aaggaattat
     2521 ctctagctgt gattcgtgca caagcagaga aacaaggatt ttattatatg gaaggaatat
     2581 tggatatcgt tggtcaagat ggttacggtt ttttacggcc aatcaattat ggaccaagtg
     2641 cagaagatat ctatatttct gcctcacaaa tcagacgatt cagtttgaga aatggggaca
     2701 aagtggcagg aaaagcccgt ccacctaaag aatcggaacg ttattatggt ttgatgcacg
     2761 tggaaagcgt taatggaaaa gatccggaag aagcaaaaga acgtccgcat tttccagcct
     2821 taacacctct ttatccgcaa agacagcttc aactggaaac aacaccgcaa agactatcta
     2881 cccgaatgat cgatatcttt tctccaatcg gttttggtca gcgtggattg atcgtcgcac
     2941 cgccaaaagc agggaaaact tctgtattaa aagaaatcgc aaatggtatc accgaaaact
     3001 atccggaagt agaattaatc gttctactga ttgatgaacg accggaagaa gtaacggatc
     3061 tagaacgaag cgtgaaaggc gacgtggtat cttctacatt tgatctgcag ccgcaaaacc
     3121 atactcgagt ttctgaatta gtattggaac gcgcaatgcg tttagtcgaa gataaacgag
     3181 atgtcgttat cttgatggat agtattacta gacttgctcg ggcctataac ttagtcgttc
     3241 caccaagcgg acgtacattg agtggcggga ttgacccagc cgcattgtat aaacctaaga
     3301 aatttttcgg ggcagcaaga aacatcgaag aaggtggcag tttgacgatt ttagcgaccg
     3361 ctttagtcga tacaggaagc cgtatggatg acgtgatcta tgaagaattc aaaggaactg
     3421 gaaatctaga attacaactt tcacgtgaat tatctgaacg tcggatcttc ccagccatcg
     3481 atatcaaaaa atcaggtacc cgaaaagaag aattattgat gccaagtgaa gaattagaag
     3541 aaatttggaa acttcgaaaa aatatgactg gagattcatt agaatatacg gaacaactga
     3601 tccgcttttt gcgtaaaaca aaaaacaatc agcaattttt caaagaattt caagatgtct
     3661 cctttggtaa aaaaaatccg accagaaatc tgaaaagata attgcaacct tgactgaaac
     3721 atgttaagat gttaatgttg taaatcgaca aatcaactct ggttcagcaa atggagcagg
     3781 gcaaaggagc gaaaaaatca tgaaagaaaa catccatcca gattaccacc cagtagtatt
     3841 catggattca acaactggtt tcaaattctt gtcaggttca acaaaaactt cacaagaaac
     3901 agttgaatgg gaagacggta atacataccc agtcatccgt gtcgaagtaa cttctgactc
     3961 acatccattc tacactggac gtcaaaaatt cacacaagca gacggacgtg tggaccgttt
     4021 caacaaaaaa tacggtctca aagacgcaaa cgctgcggaa taataaaaac gctgataact
     4081 caacgtttag cagactatct gatagggata gtctgttttt ttatgttttt tgccaattta
     4141 aaacttcgtt tgatgctgaa aaagtcgtag gatcataatg cacaaaacaa tgaaaaacta
     4201 gtatatagtt ttaattatcc acttgataaa aacaccttat attaacgaaa tgaagagggg
     4261 ttatttcgaa gtaaaaaacc atttacctaa aattagaagc taagctttcc tttcaaggaa
     4321 ggctttttgt tttgttatag acttgcgatt gtttgtgagc tagatcgttc attttaatga
     4381 cttatacacg cggatgatgg gtctttttcc tctgttcatt cataatattg aacggtacag
     4441 ctagcgatgc tatactgacc tcagttccaa gaaaacgtta acattttttg tgggggagag
     4501 gttttttatg aggtttgatc aattgatcgc agaagaactg attgttttcg atccatcgat
     4561 agattcaaaa ggatcattat ttgagcgaat aggggatgtg ttgaaaaaaa caaataaggt
     4621 aagaaatgtt caaaaagtcg ttaaggcact attcaaacga gaggaggaga tctccacagg
     4681 aatagaagat ggatttggga ttccccatgc aaaaagcaaa tatgtgacaa aaccagcact
     4741 gtgttttttt catacagcca aattaacgga ctatagcggg atagatgaaa tgccaattga
     4801 atatattttt gccatcgtag ttcctgaaaa atcagcagat atgcatctag agattctcag
     4861 cacactctcc agacgattga tgaatgcaga ttttagaaag ggaataaaag aagccactac
     4921 gccagcagat atcatcagac agattagtca agaatgaaaa tagtagagtc tttgtccgag
     4981 tatcgatagt gaagaaaaag gaggaaaata atcatgaaaa tcgttggtgt aactgcttgc
     5041 ccgactggaa ttgctcatac ttatatgtcg gcagaaaaat tagaagtaac agcaagaaat
     5101 ctaaaccatc aagcaaaatt cgaaacacaa ggtgtaaaaa cagaaaatca gttatctgaa
     5161 caagaaatca aagaggcaga tgcaatcatt cttgcagtag ataaagaaat cgaattggac
     5221 cgatttgccg ggaaaaaagt aaagcgtgtc tctacaagcc gagcaatcaa agaaccgcaa
     5281 gtagtaatag acgaagcatt gagagatatt ggcgtattcg ttgtgtctaa tgaaaaagaa
     5341 ccagctgcaa atgagaaaaa gccgacaatc tataatcatt tcatgaatgg tgtgaattac
     5401 atgcttccat ttgtgatcgc aggcggaatc atcatcgcac tgagttttgc ctttgggatt
     5461 acagcggctg atcccaactc agcagactat aatgtgttag cagctgcatt ttcaaggatc
     5521 ggaggagata cagcgtttgc catgatggtt cctgcgttag gcgcggggat tgctacatcg
     5581 attgctggaa aagcaggttt tgccccagga attgttgccg gattactggc ctctacaggt
     5641 ggttcaggtt ttcttggcgg aatgattggt ggcgtgctag cagggtatat ctgtgatttt
     5701 ctggcaaata aaattagcgt aaaaaaagaa ttttctgcta tttatcagct gattgtcgtc
     5761 ccctttttat cgatcttaat tattgggctt ttgatggtat ttgtgattga acaaccgatc
     5821 acatgggtgt tggatgcttt gacaaattgg ctgaatagct tgggaaatac atccgggcta
     5881 ttgttcggat tacttatcgg gatcatgatg gcagctgata tgggtgggcc gatcaataaa
     5941 tcgacttcga ctttctcgat cggtttgatg tcagctggag taaatgcacc gattgctgct
     6001 tgtatggctg caggaatgac acctccccta ggtattgctt tagccacttt attattcaaa
     6061 cataaattta ccaaagaaga acggacttca ggtcaatcat gctggatttt aggcgcttcg
     6121 tatattacag aaggtgctat accgtttgcg gtatctgacc cgatccgcgt gatacctagt
     6181 ttgatgacag gttcagcagt cgctgcaggg atttccatgg cagtaggagt tacttcaatg
     6241 gcacctcatg gagggatttg ggtgatgcta attccaaatg ttatgaacaa tttacctatg
     6301 tatgtcttgg caattgcatc aggtacagtc actacagcct ttatgcttgg actattgaaa
     6361 aaaacagtga aagagaaaaa acaagacaag tggacgaaaa atgaaaatct aacaaatgag
     6421 gtggaagcgt aatgttatat acaatgaaag agttattgaa agtggctaaa gagaatcaat
     6481 ttgcagtacc agccttcaat atttgcagct atgatatgat gaaagcaatc atcgaagaag
     6541 cagagagatt agaagcacca gtaatcttag aaattcatcc tgatgaaatc gcctatttaa
     6601 gtgatgagtt tgttgcggca gtaagagcct atgcttatac aagtaaactg ccgatcgtta
     6661 tccacttgga tcatggcggt acgatagaag atgtcttacg ggcgattcga aacggctatt
     6721 cttccgtgat gatcgatgcg tctttgaaat cattcgacga caatgtacag attacgaaaa
     6781 aagtggttgc gttagctcat gcggtaaatg tctcagtgga ggctgagctt ggaacgattg
     6841 ggaataatgg ttctgctgaa ggaggtgctg aaacaatcat ttatacagat cctgatcaag
     6901 cgaagatttt tgtgaaagaa acaggaatcg atacacttgc tgtagcaatt ggaacagcgc
     6961 atggcttgta tccgaaaaac agaactccaa aattaaatat cgacttactg aaagaattga
     7021 accaacaaat cgatcttcca tttgttttac atggcggttc aggtaatccg gacgcagaag
     7081 tcagcgaagc agtaaaatac ggagttgcca aagtgaattt gagctcagat cttaaaagcg
     7141 tctttttcaa tgctttgaga gaaatattga gtgaaaatcc atcgatgtac gagccgaatg
     7201 caatttatcc agcagctaat aaaaaggtac aagaagtggt caaacataaa atggaaattt
     7261 tgaacacagt aggcaaggct agtttatact aaaaataaaa atgtgtggtc aagagggtat
     7321 gacaatagtc ttgtcatacc ctcttaacca cacacgatgt tcagaagtag ttttttcgaa
     7381 aaatttcttc ttatttctcg aagccaatca cttctgtcac aacctctttt ttcctgttac
     7441 tatataaagt aaatggggtg attttatgtt gaatgaagtg gagaatagac agcaggagat
     7501 tgtgaattat ctaaaaaaca accaatttgc aagagtcacc gatttgatcg agttagtaaa
     7561 ttatagcgaa gccacgatta aacgtgacct tgtttttctg gaaaaaagtg gattgatccg
     7621 tagagttcgc ggtggcgcca tgcttattga caatcgaaaa attgacgtgc cttacctgat
     7681 gaagatcacc aaattagaag aagaaacaga aaaacgtaag attgcagata cagcttcttc
     7741 tttgattcga gatgatatgg tcttattctt ggattccagt acaacctctt tacacttagt
     7801 cagaaaccta gcaagatttg atggtttgca gatcattacg aatggcatcg tcacagctgc
     7861 tatgttgagt gaatatacaa cggcaaaagt atctgtattg ggaggggcaa ttgttccgaa
     7921 acgagcaaca atcaacggtt caaaagccta taacgatgca ttgtcatatt atgcggattt
     7981 agcgataatg agttgtcgcg ggctagattt cagccaagga gtaacagaaa ctcatgaagg
     8041 agaggcaatg gtcaaaaaag cttttagaaa acaatcagac cggcttgccg ttttagttac
     8101 acagcaaaaa attgcacata aattcatgca tcaagccatt gcttttcatg aaatcgatta
     8161 tctggtgaca agtaaagaac tgacaaaaga acagtatgcg aaagtaaagg aacaccatat
     8221 cgaatgtctg tacggataaa ttattacgtg aatgttctaa gtggcttcct catttttaga
     8281 taggacctta tctttgctcg cttctctcag ccgatggtat aataatgaaa aggttatctt
     8341 gttagataat cttacttttt tatccaggag aaaggagtgc agaaaatgaa acatgtgaag
     8401 gcgttggtag tgaaggcaat catgatctgg gcgattctgt gggtcgtgct gacaggttta
     8461 tacggtgtta gctttatgga ttcaacgatt gttggggtaa ttattgtagt catgatctat
     8521 gtgctagggg atctgttgat cttacgtaaa gtagggaaca tcgcagcgac gatcgcagat
     8581 gctggttcag ctgcagttat cttgtggctc tatctatatt ttatgaatga tacagtcgat
     8641 atttggatga aagtattgat tcctgctcta ttgatcggtg tggcagaatg gttcttccac
     8701 aagtggttgc taagtcaagg catcgtacca gatgaacgga aaatggaata aatcaacagt
     8761 taaactattc ttatcgataa atgaaagctt taaaaataaa tagatgttta attaagaata
     8821 atcataaact atttttactc aaaaaaccgc tcagattatt ttcagatctg aacggttttt
     8881 tttatctatt catcaagatc aaatttcatt tttcaatgat tcaatcaatt cgccctctgc
     8941 ttcatgacta tgttttcggc cattaacaag atagagtgct gtacatatca atggaatgac
     9001 tccggcaatc acatacaatc ctctaaatga agtaagagat tttagcatcc ccatcaaata
     9061 aggaccaacg cctaagccta ggtctaaacc aataaagtag gtagataaag caatactgat
     9121 tcgatgttcg cggacgattt ttaaacaaac agcctgtcca tttgacataa acgtaccata
     9181 acctaggccg actaatgctc cagagataag caatgtcaca ctattcgtcg ttacactcag
     9241 taaaaataat cctgctgtta agaataaata gctaggatac atcacgtatt gttctccttt
     9301 gacatcgaaa atccttccag tcaatggacg tgtaaatgtg atgaccaaag catatacgac
     9361 gaagaaaaat gacccagcag caactaagtg gattactttt gtataagaag ataaaaatga
     9421 taaaacactt gaataagaaa gacccatcaa aaaggcaatt cctgtaatga aaagtgcttt
     9481 cttttcaata aaactattga tattccatga gcgaagagat attttttgtt cttcagataa
     9541 tggaatattt tttacaggaa agacaaggca ggcaatcgta acaaagaaga ccagtacaat
     9601 tgaaaaccag ataatgaaac gaaaatcagt catattcagc aaaatcattc ctaaaaatgg
     9661 cccaacagcg gcagctaaac tagtacttaa tccataataa ttgatgcctt cccctttttt
     9721 agatgcaggg atatatgcag taacgatagc atttgtagct gtagaaactg taccgtagcc
     9781 gaatccattc aagaatcgaa ctaaatacaa tgccccgata gttggcgtat aaagatatgc
     9841 taatgttgaa aggagataga agatcgcgcc gcctcttaag gtaaacttcc gaccaaatag
     9901 ttctaactgt ttccccatga ataagcgtgc cagcaatgtt ccgatgatat agatcccaga
     9961 agcaaaacct gcttggctca agctggcgtg taaggtgtct tgcgcaatga ctgccatgat
    10021 gaccatcaac agataataaa tcaggtagac aataaaattg atcaatgtga ttccaataaa
    10081 atatttatta aataatttct tttccatgta tgttatacat tccccttaag ctgaatatgc
    10141 ttttaatttt tgtatttgaa cttctacatc gtgataattg gatggatagc gatcaacaag
    10201 ttggcgaata aataaatctc ttgtagacat aaacagattt aaaaattctt cgcattcgtt
    10261 tgttggattc tcatccactg ctttgatcaa ttcgatcgag ttttgatacg catcatttaa
    10321 aaagactaaa gaagctttca tatcaaatct tgtttgatat gctgtttcta atcgattaat
    10381 gattgtgttc atccagtaat tcatgttttt tccctctatt cttaaaaaat tttacttctg
    10441 cagtataacg aataaaaaaa aggaaaaaaa tcgcataagt gatggaaaaa ggtacttaac
    10501 aaatgttaat atcattagta aaataccttt ggactttaaa aagttttaaa gtactgtatg
    10561 atctttttag atgaaagttt ggatggggga gcatcattgg atagtagagt cttaaaggaa
    10621 tatttggata aaaatgattt tccgattata cgcaaaggat atcataaata tttgacattc
    10681 gaagggatgg aagattccca tacgtatatc ttgaaagagg gaacggtaaa agcaagtgtt
    10741 atttcaaatg atgggagaga atttaacctt cgctatatca aagatcttga aatcgtctct
    10801 cttttaaaag acgaatattc acaattcatc gattcgccgt ataatattcg agttgaatca
    10861 gaatatgcag agttttatcg aatcaatcgt gtgcgctttt ggcaaaatat caaccaagac
    10921 ccaaagctac aacactatgt aaaagagtat taccgctatc gtctgcttta ttcgatgaaa
    10981 aaaatgcagc aaatgctatt aaacggaaag ctgggagctg tttgtacgca actttacgaa
    11041 ttatatgatt tattcggtgt gcggacagaa gaagggatgc tgatcgattt ttcgatcacg
    11101 aatgaagaaa tcgccaaatt ttgtggcatc tcaactgcta gcagtgtaaa cagaatttta
    11161 aaacaactga aaaaagatgg tattatcacc gttaaagacc atcatatcgt tattttgaat
    11221 atggacttgt tagaagacaa tattatcttt tgatgaacaa agaggttgtg aaatcagcgt
    11281 tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt gccgtttttt
    11341 gagctaatac cgagggtcag ctgattgaac accgtttacc aagaggttgt gagcctgccg
    11401 tttaactctg agtcacaagg aacaatttca aaaaacagct tttcatgttt gatgaatatt
    11461 gtgacttgtg accgaggagt tggtaggaga acaccgttta ccaagaggtt gtgaaaaagc
    11521 tgttctgcat caagtaataa gaagagccaa gtaaaaacag tctcttatgt ttttaacttg
    11581 gctggactta tcaccgtaga tgtaagcttt tgaacgccgt ttatttgcaa caaaaggggc
    11641 tgtgacacga tttttgtcac agcccttttt ttgcgaacat tcatttagca atttttagaa
    11701 acttctgccg ccaccgccaa aagtcccgcc accgctagaa tgggtagtac ttccgccacc
    11761 gccgcttcca ggaggtgggc ttttggggat tcttcttgtc gtgacaaaag aatttgtcag
    11821 acgatcggtt ttttcagtta atttgatact ggatttttct cggaatggat atttgtagat
    11881 gcctgatttc aattgataac gcgaaacagt cacaaaataa aagacaagac taaggataag
    11941 agccaaaaca acagcaatga tgatttctac tgtcgtcaaa accttatagc gtgtaatttt
    12001 gccagtttct tcgtctacac gataatgtcc tcctggaact ccgtcttcca catatgttgc
    12061 tgatttagac aggtaagctt ttgcagcatt gtaataggca gcatttgtca tctgatcata
    12121 aacatcatct aagatcgaat cgattctgct atcagtcaaa taatcgatca tattaccaga
    12181 agtcgaaatg tagatttcac gctgtcccat atctaatagc aatactgagc cattttgatc
    12241 atttccgact gcgtctctta agtaattatc agcaaagtct cttggttctt ctgaattaga
    12301 tgtagtcgtt acgatcaaga ctcgtccttt gattttttca ttcagctttt ctgcctgttg
    12361 ttccaaagat tggatttgat ctgttgtgaa taacccagcc tcatcattta ccgtagctgt
    12421 atctgcatga gtagccaccg gccagccaac acttactagc aaaagaaaga ataaaaaaaa
    12481 ctttttcatc atatcaagta acctccgatc agaaagagga tcgctagccc agcgaaaatc
    12541 ccaagagcgg tcagtcccaa ttttttctgg ctgatcggca ggacaccgct gacctttcct
    12601 gtttgtccat tcattgcgta gtagtagact tttttacttg agtcattgct tcgataagta
    12661 acaagccata ccggcagtaa tacgtaatta tttacttcgt ttgtgattga agcagaagtt
    12721 cgcacaccgg tcaatgttgt atatccattt gcggtatctt ttaacagctt ctctgaatag
    12781 tcttttaatt cagattgaat ctgtgatttg attgcctcat attcgatatc tcttttttct
    12841 gcttggaacc cggctaaata ctggctttta aaatcaattg ctttatctaa cgggaaaggt
    12901 tgcacgcctt cgaccatctt ttgttgcgta ttttttgata aggcattttt tactagttct
    12961 ttaaaagaaa gcttgcctgc tcttgaaaca gcaaattgct tcgtttctgt gtattcgatg
    13021 tcacctatcc gccatatgcg gataactgta ccattcgcct gcatggcacc atctacttca
    13081 gcatctgtag cccaataagg gaaatacaca cctgtcaact tatcgatttg atccttattg
    13141 aaaaaagctt tgggaacaaa ccattttttc ttggtccaag cgagaaatct gttgattgct
    13201 tcttctttct cgacagcaaa aggaagtact ttatttggca agaattctcc gcttaaacgt
    13261 cccgatagta cgacgggatt atggcagtag taacaataag tcgcagcagt tgtcgcatca
    13321 gtcacgatct ctgcaccaca gttgggacaa agaaaaagat ctatcgctgc ggtattatct
    13381 gcagattcgt ccgctgtatc tgaaaaaccg cctgcctcgt caaccgcttt gcgctcggct
    13441 tcattcatat cggcaagctg ttcttgggct gtaaaagtaa aatcttcggt aggtgaaggt
    13501 tcttctttgc tttcttcgcc agccatacgc gcttcttttt gcttttgctc atattggctg
    13561 acttcttctt ctgtatagat attcaggcag tattcgcaat gaaatttttg gtctttggga
    13621 tcaaaagtca acggaccgcc gcaattagga catttatgtg taagaacatc catcagctgc
    13681 acacctttct aaataaatta attaacgggg attcctttaa acggtttacc acagtttgga
    13741 cagaatttag ggatgccgtt ggaaagatcc acgatctcat tgcattcact gcatcgcatt
    13801 tgtagcttag gctgaacgtt ttctacaggt ttggcagctc cgcagttgga acagaatttt
    13861 ccggaatttt ctgtcccgca aactggacat gtccaagtat ccgcatttcc ttgtgcagtt
    13921 tgttgatttt gtttttctgc ttgttgtttg atttgttctt ggttattttg agctgcttga
    13981 gaaagataag aaccatttgc attcattccc atacctacac ccataaagcc ggtcattgct
    14041 cctgcttcat tttttccggc tgcttccata ccacgtgcaa tggaaccttg cacgtatcct
    14101 tcacgtacgc taggatcacc tagcatcgcc ccttcgttac gcatattgat cagtttcaca
    14161 gaatcatcgg tgtaagaaat gctggctaca gccacagaaa caatctccat cccacgtaat
    14221 tcacgccaag aatcatccaa tgcagtatcc atgtatttgc ttaattccaa actcttagat
    14281 ggtacataag aaatccgttg accatctgca gacatctgat tgattgctga ttgtagcgct
    14341 gttaggaatt cagctaaata ctgctcattg atatcattga tttccacttg tgttttgttt
    14401 ttagggattg cgtttgtata aaataaaatc gggtcaacga catgaataga ataagtgcca
    14461 tgtgcccgta agaataattc tgcgttatag aaattgtcaa agtagttcaa cggcgcgctt
    14521 gtaccgaatt tgattccctt tatttcttgg agattgatat aaaaaacttg ttgtttctgc
    14581 ggtgtgacgc cgccaaattt gaatcggtca aaagtttcag caattgcttc tttcagtgtt
    14641 ccattgaaca tggaaggagc tgcgtcattt ttaatggtgt aataaccttc ctcagcggta
    14701 taatcgatga tttttccacc atcgaccaaa agcatcatca tgtttggata gacatggacg
    14761 actgttccgt ctgttaaaac atcttctgtt cctttacgat tggagccacg tttgtcatct
    14821 ttgcggactt tgacaccttt tgtcatgacc gttgtatcac tcatattgtc tggttcgatg
    14881 acttctagcc attgatctgc cagaccgccg ccaaccatac ttgttgctgc tttgataaga
    14941 cccataaaaa attcctcctc tgattcagct agtgccaaac aattattgtt tatttattat
    15001 accataggaa gatgtgaaac ttctcggact agagacggaa ttgaaatgga aaattgcgta
    15061 ttgtttaaat acctgaaaaa gccgagaagg atagtagttt ttttacctaa tatcacgtat
    15121 aataagagat agtgaatttt ttaaaaaagg gggatcctta tggcatatca agcactttat
    15181 cgagtctggc gttcgcagcg tttcgaagac atcgttggac aaaaagctgt gacacagaca
    15241 ctaaaaaatg ctattgtttc ccacaaaacc tcacatgcct acttgtttac aggacccaga
    15301 ggaaccggaa agacgagtgc agcaaagatt ttcgcaaaag caatcaactg tccgaacagt
    15361 aaagacggtg agccatgcaa cgaatgcgaa atgtgtcgat caattactgc tggtacgcaa
    15421 gaagatgtga tcgaaatcga tgctgccagc aataacggag tcgaagaaat ccgatttatc
    15481 cgtgatcggg caaattatgc gcctacaaag gcagcgtaca aagtgtacat tattgatgag
    15541 gtacatatgc tttcaacagg agctttcaat gccttgttga aaacattgga agagccaaaa
    15601 gaaagtgtca tattcatctt agcaacgacc gaacctcaca aaataccagc tacgatcatt
    15661 tcacggactc agcgatttga ttttaaacgg atcaatacgg cagatattgt cgaacatctt
    15721 gaatttatct tgaaagaatc gactatcagc tatgaaccag ctgcattgaa tgtgattgcc
    15781 cgttctgcag aaggcgggat gcgggatgca ttgagtattc ttgatcaggc aatctcgttt
    15841 agtgaaggaa caattacttt agatgatgcc atgcaagtga caggtagctt aactgacgaa
    15901 atgatggaca gttacttgag tgcttgcgtg aaaaaagaag taccagaagc attggaaaca
    15961 ttgaaccaaa ttctcgcagc cggcaaagaa tcacgtcgct ttctagagga tatgttacaa
    16021 tactgccgtg acttgctgat gtatcagcaa gcacctaacc tagttgagga acaatcgagt
    16081 acgatcagaa gtacatttac agaattggca caaactgttt cagcggatca gctgttttca
    16141 atgataaaga ttttgagcga tactcaaaat gaaattcgtt ttaccaatag tgccgctatt
    16201 tatttggaag tcgctacagt caaacttgca cagctaccgt ctgcttctgt tccttcagca
    16261 gatgtagcat ttgcagatga tcagatagta aaacagctac aacaagagat taaggaacta
    16321 agaaacgaaa tcaaggaact acgcagtaat acaggaattg agaatacaaa agtacagccg
    16381 gtacagcaaa caaatcataa aaaacagaca agtacctatc gtgtaccgac ggaacgagta
    16441 tataaagttc tgcgggaagc gacaagacag catctgaacc aagtaaaaga agtatgggaa
    16501 gacttgctga tgagtctatc tgtcactcag cgagcaatgc ttaaagcaag cgaaccagtt
    16561 gcagctggac ctacaggact agtgattgcg tttgattatg agattgtttg tcaaagagca
    16621 gcaaatgacg aagaattgca gctttcggta cacaacaact taagccgaat gatcaaagat
    16681 tatgcgcctg atcctgttta tattacaaga gaaagctggc cagaacttcg aagaaattat
    16741 ttgacgcagg cacaagaaga gggaacagaa aactcttttg cttctgaaga ctcagaagga
    16801 gaaatcgagt tgcttcctca agaacaagaa gtaccagaag aaacacttgt agtaaaagca
    16861 gaggaactgt ttggctccga agctgttaca gtggtagatg attaataaaa aggagtagat
    16921 aattatgatg cgtggaatgg gcaatatgca aggcatgatg aaacaagtac aaaaaatgca
    16981 aaaagaaatg gcagaagcac aagagctgct aaataaaaca gagtttacag gtaaagccac
    17041 caatgaattg gtcaaagcgg tttttacagg cgatcgtcgc atgaaagata tccaaatcaa
    17101 tgaagaagtc gttgatcctg atgatatcga gatgcttcag gatcttgtcg taatggcagt
    17161 caatgatgcg atcgcacaaa ttgatgtgga aactgagaaa aaattaggga aatacgcaaa
    17221 aggtttgcca ggattttagt tttaaactga aggagtaatg aatatgcaat atccagaacc
    17281 aattgcgaaa ctgatcgaca gctacatgaa acttccagga atcggtcaga aaacagcgac
    17341 aagactcgcc ttttatacga tcgatatgaa agatgaagta gtaaatgaat ttgctaaatc
    17401 tttgttaagt gtaaaacgcg atttgcattt ttgtagtatc tgcggaaaca tcacagaaga
    17461 agatccctgc gaaatatgta gaaatccttc acgtgatcga agcgttatcc tagtggtcga
    17521 agagccgaaa gatgtaatgg caatggaaaa aatgcgagaa tatcacggac tttatcatgt
    17581 tctccatggt gtgttatcac cgatggaagg gacaggtcca gaagacatca atatttcttc
    17641 tctgttgcag cggctgcatg atgcagatgt aaaagaagtg attatcgcaa caaatgcaac
    17701 aacagaggga gaagcaacag ctatgtatct ctcacgtctg atcaaacccg caggtatcaa
    17761 agtgactcgt ctagcacatg gtctgtctgt aggctctgat atcgagtatg cagatgaagt
    17821 tactttattg aaagctgtcg aaggaagacg tgagttataa atttctaatt aagtgttttt
    17881 tcagatacaa tataaaagca gcacaagaga tgggggcaac aatgtgaacg gactatttat
    17941 tacaatcgag gggcctgatg gtgcaggaaa aaccagcatc ttgaatgaac tttaccctct
    18001 tttgaaagaa gtagcaaaaa cagaaatcat tcaaacaaga gaaccaggag gcattccgat
    18061 tgctgaagag atccgagcag tgatccttga tccaaaaaat gatcggatgg atgaacgtac
    18121 ggaagcactg ctttatgcag cagcaagaag acaacatcta gtcgaaaagg ttctgcctgc
    18181 tctggcaaaa gggaaaatcg ttttatgcga tagatttgtc gacagttctc ttgcttatca
    18241 aggtgcaggc cgacgcatcg gcgttaccga gattgctcgg ttgaatgagt ttgctacaga
    18301 aggtactacg ccggatttta ccctttatct tgacgtagat tcagacaccg gattaagaag
    18361 aatcaagaaa aacagacaaa atcaaatcga ccgtttagat tcggaaggtt tggaattcca
    18421 ccagcgtgta cgtcatgctt acttaaagct ggcagaagag aatccggaaa gaatccacaa
    18481 agttgatgca agaaaaagct ttgaagaagt gctgcaaaca agttatcata caattattga
    18541 acagtatccg caattttttg aaaactagga aggtgaacgt ttatgaaaat tattttagcg
    18601 attatccaag acaaagacag caaccgttta tcaaatgaat tgattgatgc aaatatccgt
    18661 gccacaaaac tatcatcgac aggcggcttc cttaaggctg gaaacagtac gtttatcgtg
    18721 ggtattgaag atgaccgtgt agaagaagca ttggaaatca tcaaaaaaac gtgtgaatca
    18781 agaaagcaat ttgtatctac acctgttaca ctggatattt cgatggatgg gggcgtccct
    18841 tatccagtag aagtagaagt aggcggtgcc acagtcttcg tcttacctgt tgaaggattc
    18901 catcaatttt aggaggagct gagctatgaa tcaagaaata aacttggctc aaatgcaacc
    18961 gatcgtgtat cagcagcttc aacggagttt tgagcatggg cgtatcgctc atgcttatct
    19021 ttttgaaggg gagaaaggaa caggaaaaca tgaagtaggt atatggcttg cacagcacct
    19081 gttctgtacc caaatgaaag acaatctgcc ttgtggaaaa tgcaacaatt gtcagcggat
    19141 cgaaaagaaa gaacatccag atgttttggt tattgaaccg gaaggacaaa ccatcaaagt
    19201 cgatcagatc agacgtttgc agacggaatt cagtcgcagc ggttacgaat caagaaaaaa
    19261 agtttttttg atcaaagaag ctgaaaaaat gaatagcagt gcggcaaaca gtttgttgaa
    19321 attcttggaa gagccccctg gagatttttt agcggtgtta gaaacagatg cagtaggaag
    19381 aattctccct actattcaat ctcgttgtca agttctgcat tttcaagaac tttcgaaaga
    19441 agcactgatc catcgattgc aagaagagca gattccattg gaaaaagcaa aacttttggc
    19501 tttcttaaca aacagtttag gaaaagcagt tgaaatctct caagatgaat ggtttaatga
    19561 tgctaaggac ttgatttatc aatggttcgt ttacttgcaa aagcaagata ctcaagcctt
    19621 tatctatgtc caaaaaaaat tggtaaaatc atttaaagaa aaaacacaac agtttacagg
    19681 attatctgtc ttgatgtttt attatcagga agcacagcgt caagcagtag caaacggcga
    19741 atatggcaaa gtgaaacaga taaaccagat aattgaacgg atattgatcg cagagcagaa
    19801 actacgcagc aatgtcagtt ttcaggctgt tgcggaacag ttcgttttgc atacgatttt
    19861 ttagcttgcc gcaaaggatg atgatcaagg aggatggaaa tggtagaagt agtaggtgtc
    19921 cgatatagag atgcaggtca tatctattat tttgcaccag gagactctga atatgtttat
    19981 agtgacaagg tattagtcga atctcagcaa tccttacata ttgctactgt tgtccttcct
    20041 aaaaaagaga ttgatcccaa tgaccttcct gaagctatcc gtccgatctt gcataaagcg
    20101 tcacaagctg aattagaaaa agacaaaaaa aacaaaaaag atgcttatga tgcattgaaa
    20161 atcgcaaaag aaaaagtgca gttgcgtgag ttggaaatga aaatggtcaa agccgaatac
    20221 acatttgatc gaaataaatt gattttttct ttcacagcgg acgggcgaat tgactttaga
    20281 gaattggtaa aagatctggc cgctgtattt cgtactcgta ttgagcttcg acaaattggt
    20341 gtccgcgatg aagcaaagat aataggcgga attggtccat gtgggcgtcc attatgctgt
    20401 tcttcttttt taggcgattt cgtgcctgta tcaatcaaaa tggcaaaaga tcaaagttta
    20461 tctttgaatc caacaaaaat ttccggttta tgcggacgct tgatgtgctg tctgcaatat
    20521 gagagcgaag aatacgagac agcgaaaaaa gaactccctg actttggaaa agtaattgct
    20581 acacctgatg gtaaagggaa agtcgtaggg ttgaatctgc ttagccgtat cgttagtatt
    20641 cggctgactg gacgggaaaa tcctgtcgaa tatgaatggg atgaattgaa agaatttatc
    20701 caagtaggtg agatgaatgg ataagagatc attatacgac ggattgaatc aattagaatc
    20761 agagttgaga aatacattaa cagaacttgt tgaaatgaag catgctttgc atgagatcgt
    20821 agaaaaaaat acaactttgg agatggaaaa tcaacgtctg cgggaacacc tgcgggaatt
    20881 gaaccaagtt gcacaagctc cagctgaaaa tgtgaagcag gaactttcta agtctcgtat
    20941 gaatcttgaa aaaatttatg aagaaggatt ccatgtctgc tacgatttat atggatcaag
    21001 aagggacaat gacgagcctt gtgcgttttg tctagaagtg atctatcgcg aaagcggtcg
    21061 atgaccaata acaagaggag actggagcgg agaaatactg ctccagtctt tttattaagg
    21121 agattgtgac agatgtataa tcaaaaaagt ttcaagggaa atccggtata tggggattta
    21181 tatcttgtac cgacgccaat tggaaattta gaagatatga gctacagaag tgttcgtatg
    21241 ctgcaagaat ccgatcttat cgcaagtgag gatacgcgta atacacaaaa gttattgaat
    21301 cattttgaaa tccatacacc ccaaaaaagt ttacatgaac ataattataa agaacgtgtc
    21361 cctcaattga ttgctttgtt aaaagaagga aaaacgattg ctcaagtcag tgacgcagga
    21421 atgccttcca tcagcgatcc tggacatgag ctagttttag cctgcataaa agaagggatt
    21481 tctgtgatct ctatcccagg acctacagct gggatgacgg ctttgattgc ttcagggcta
    21541 gtgcctcaac catttttatt ttatggcttc cttccacgta agaaaaaaga acagaaagaa
    21601 acattgaaaa agctaaaaga acagacagtt acgctgattt tttatgagtc accttatcgg
    21661 atcactgcta cattgacgaa ttttttagaa atttttggaa atcgccaagt cgttttgtgt
    21721 cgggaattga caaaggtcca tgaagaatac cttagaggaa gtacggaaga gctactcgct
    21781 tatatcgaag aacatccagt aaagggtgaa tgttgtctgc ttgtagaagg gaatttgttc
    21841 gaaatgccag tgacggaagt tgatgaagag ttgggaacgt taaaggaaca agtagaagca
    21901 aagatcgctt caggcgaaaa accaaatgct gctatcaaag cagtagcgct gaaaaatggg
    21961 ctgaaaaaac aagaagtcta ccgtcagtac catgaattag actgagatag tataagttgt
    22021 ctataatgaa agactaatga ggaaatcagc tactaacttt tcaaatctct tgaacgcgaa
    22081 cgaatgttct tgtataatga ataagaggtg aggatgatgg agctgcaatt tcctttacat
    22141 aatgatacat caagaaaaat cattcatatt gatatggatg cttttttcgc ttcggttgaa
    22201 gaacgagatc atcctgagtt tcgcggacat ccattagtaa ttgccagaca ccccagtgac
    22261 actggtgggc gtggagtagt gactacagca aattatgaag caagaaaatt cggaattcat
    22321 tcagcaatga gtgcacaaaa agcgtatgag ctttgtccgc aagccatctt taagcctggc
    22381 gaccatcata aatacagcga tatttccaaa caagtacggg aagtattcta tcgttataca
    22441 gatattgtgg aaccagttag tatcgatgaa gcgtatcttg atgtgaccga aaataaaatc
    22501 aattgcaagt cagccatcaa aatcgcccga atgatccaag cagatatttg ggagagtgtc
    22561 cagctaacat gttctgcggg agtaagttac aataagtttt tggccaaatt agcatcagac
    22621 tatcaaaaac ctagaggaat cacggtagtt gcccctcaag acgctgtgcc atttttaaaa
    22681 gctttgccaa ttgaaaaatt tcatggggta gggaagaaaa ctgtacctag aatgcatgag
    22741 ctagggattt atacaggaaa agacctatat gaatgcacag aaatgatgtt gattcgaaat
    22801 tttggaaaaa tgggctattc tctttatcgt aaagtcagag gaatccatga ttctccagta
    22861 aacgttacaa gagagcgaaa atctgtggga aaagagcata cttatgggca gccgttgcaa
    22921 acggaagaag cagtgctgac tcagttgcgc caactagctg aaaaagtaga agaagcactg
    22981 agaagagtcc aaaaacatgg gaaaacagta gttttgaagg tcaggtacac ggactattct
    23041 actgtgacca aaagagtgac attgccagaa tatatctata aaaaagaagc actcttttat
    23101 caagcaagcc ttatatggga ggaaatcttg ggagtagaaa aaggaatccg attgttaggg
    23161 attactttga cgaatctgga cccgatgact tatgaaaaca tcgtccttcc attatgggaa
    23221 aatcaagaaa tataaaaaca gctttgagag ttgaaaccgg aaatccgttt tctctcccaa
    23281 agctgttttt atatcaagtc ttgctttttg atttgttcat aggtttctgt tgcatccgta
    23341 catttttccc aaatcacaac ttgattggat tctaaagatt tttggacgtc aatgatccgc
    23401 tggttgctac ttccgcgaaa ttgtagtttt aggtcacgtt tgcttaattc aaagcgtccg
    23461 tcaacaagga tatcgatttg cgaaaggagt tctaatttat cttctgaatc ttgtagcaat
    23521 tcatcaaagg catagcctgt ccatgaccag atatcttttg tcgtaccgaa ctcttttcgg
    23581 atacgtcgta cgacttgcag acaaacatct gtattcaaaa aaggttcgcc accaagtaaa
    23641 gttaatcctt gcacgtaatc gttgcgcaag tcagcgatga tttgttcttc taattcaggt
    23701 gtgaatggtt tgccatatcg gaaattttgt gcagctttgt tgaagcagcc ttcgcaggca
    23761 aataagcagc cactaacgta aagactgttg cggacacctt caccatcgac aaaattaaac
    23821 ggtttgtagt cagcaatata gttttggctg tagtcttttg ctaaccattc ttttggtttt
    23881 ggatttctca taaaaaaact tctttcattt aaggttcaaa gatatagcta ttgaagataa
    23941 aatctactcg accacataaa taagaacaaa ctgacctaaa tccattgttc aagaaagctt
    24001 gttcttattt tgatagaggg cgttcaaaga tacaaagcca tcgaaagagg gtggtgaaaa
    24061 aagacttgtc atagcctctt attctgaata aacggtgttc aaaagcttgc atctgcagta
    24121 ataagcccaa ctaaacgaaa aatatgagga actatttttg tttagctatt cttattactt
    24181 gatgcaggac agctttttca caacctcatt tcatgtgttt cacacgtgag gaaatttctt
    24241 tatggcggcc atgtaccatc ggacgagctt gcggatttcc taaatagcca caagtacgtt
    24301 tgactacatc acatgttttt ggatcatgat ttccacattc tggacattcg aaccctcgtt
    24361 ctgtcggttt gaaatcacct tcgaaaccac attgatagca atgatcgatt ggcgtattcg
    24421 taccaagata cccgattttg tcatatgcaa aatcccatac agcttctaaa gctttcggat
    24481 tttgttggag catagggtat tcacagtagt ggatgaatcc acctgaacaa tattttggat
    24541 aatccttctc aaattccaat ttttcaaatg gcgttgggtt tttacgcaca tcatagtgga
    24601 aactgtttgt ataatattct ttatctgtga tattttcgat gatgccgaat ttttctgtat
    24661 ccaaacggca gaaacggtca gttagacttt cacttggagt tgaatagaca ctgaaatgat
    24721 aaccatattc atccccccat gcatctgcat tagcttttaa ttctttgacg atatcaagtg
    24781 tgaaagcttt tgcttctggg tttgtttccc attcgccacc gaagaaagct gaagcaactt
    24841 catacaatcc aatgtagcct aacgatacag tagctcgacg gtttttgaat aattcattga
    24901 cagaatcggt acgtgaaaga cgtttcccga aagaaccata catatataaa attggcgcat
    24961 ttgctggaat ggcttcttta caacgttcta cacgatagag aagcgcatct ttcatgattt
    25021 ctaagcggtc agacagtaac tgccagaatt tattctgatc tccttttgat tcgatcgcga
    25081 ttcttggcaa attcaatgta acaacaccta aattcattcg tccgacattt acttcttgac
    25141 cattttcgtc tttccaccct tgcaagaatg aacgacagcc cataggtact ttgaaacttc
    25201 cagttaattc aacgatttta tcgtaattca gaatatcagg atacattctt ttcgttgcac
    25261 attccaatgc tagttgtttg atgtcgtaat taggatcttc agggtttaag ttcacacctt
    25321 ttttcaaagt gaagatcagt ttagggaaga tagctgtccg cttttcactc cctaatccat
    25381 tgattcgaat ctgtaaaatt gctttttgaa tttctctttc aaaccagttt tctcctagtc
    25441 caaatcctaa tgaagtgaaa ggagtttgtc catttgaagt aaataaggta ttgatttcat
    25501 attccaaact ttgcatagca tcgaagatat cttttttggt ttttgctttt gcatattctt
    25561 tttggcgttc tggcgaatcg atccattctt ccgcgtcttt tagatgtttt ttatagttca
    25621 attctgcaaa aggagcaagc aattcatctg ttcggtcggc agaacagcca ccatattgac
    25681 ttgacgctac attggcaata atctgtgaga tttgtgctgt ggcggtctgg atagattttg
    25741 gcgattcgac ttcggcattg ccgattttga acccattgtt cagcatgcct ttgaaatcaa
    25801 tcaagcagca attcgtcatc ggtgtataag gatgataatc caaatcatgg taatggatat
    25861 cgcctttttg atgcgcattt gctatatgtg gtggcaacat tttcagtccg attgattttc
    25921 caacgatccc ggctgtcaga tctcgctgtg tattgaaaac attactgtct ttatttgcgt
    25981 tttcgttcac gacagtttgg tccttgttga ttagcttttc aatcgaaaag ttgatatctg
    26041 ttgctttact gcgagcaaaa tctcgtttgg tacgataatt gatgtactct tctgctaatt
    26101 catattcgtt tttttctagt aatatatgtt ccacgatgtt ttgaatctca tagattttga
    26161 cattttcagc aaaccggctt ttgatttctt ggacaaccaa gtctgcaatc atttgaattt
    26221 tttcataagt aagtggtgat aatgagccgt gaattttctt ttctgctttg atcagtgctt
    26281 cattgatctt ttgttcatcg aaatcaacat ggcggccatc gcgtttgatg actttcatct
    26341 gggatgtctc tggctgctga gtaacgacag ttttttcttc ataatttact ttcattatct
    26401 tcaactcctg ctaaattgtt cttgtctatc ataaaacaaa atcggggaag gagcaactaa
    26461 atatcggcta tatatagtat tcaatgtgc
//
LOCUS       DACDMM010000021        24397 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDMM010000021 DACDMM010000000
VERSION     DACDMM010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.021"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            67..444
                     /locus_tag="IWD16_000695"
     CDS             67..444
                     /locus_tag="IWD16_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087779.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            complement(624..1919)
                     /gene="purB"
                     /locus_tag="IWD16_000696"
     CDS             complement(624..1919)
                     /gene="purB"
                     /locus_tag="IWD16_000696"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP9087780.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1932..3065)
                     /gene="purK"
                     /locus_tag="IWD16_000697"
     CDS             complement(1932..3065)
                     /gene="purK"
                     /locus_tag="IWD16_000697"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP9087781.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(3049..3537)
                     /gene="purE"
                     /locus_tag="IWD16_000698"
     CDS             complement(3049..3537)
                     /gene="purE"
                     /locus_tag="IWD16_000698"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP9087782.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3830..5137)
                     /locus_tag="IWD16_000699"
     CDS             complement(3830..5137)
                     /locus_tag="IWD16_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP9087783.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(5140..5721)
                     /locus_tag="IWD16_000700"
     CDS             complement(5140..5721)
                     /locus_tag="IWD16_000700"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP9087784.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5837..5934)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6293..7585)
                     /locus_tag="IWD16_000701"
     CDS             complement(6293..7585)
                     /locus_tag="IWD16_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9087785.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7582..8205)
                     /locus_tag="IWD16_000702"
     CDS             complement(7582..8205)
                     /locus_tag="IWD16_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087786.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(8208..9044)
                     /locus_tag="IWD16_000703"
     CDS             complement(8208..9044)
                     /locus_tag="IWD16_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9087787.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(9061..9948)
                     /locus_tag="IWD16_000704"
     CDS             complement(9061..9948)
                     /locus_tag="IWD16_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9087788.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9956..11191)
                     /locus_tag="IWD16_000705"
                     /pseudo
     CDS             complement(9956..11191)
                     /locus_tag="IWD16_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(11874..12557)
                     /locus_tag="IWD16_000706"
     CDS             complement(11874..12557)
                     /locus_tag="IWD16_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9087789.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12575..13192)
                     /locus_tag="IWD16_000707"
     CDS             complement(12575..13192)
                     /locus_tag="IWD16_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP9087790.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(13202..14026)
                     /locus_tag="IWD16_000708"
     CDS             complement(13202..14026)
                     /locus_tag="IWD16_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9087791.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(14019..14579)
                     /locus_tag="IWD16_000709"
     CDS             complement(14019..14579)
                     /locus_tag="IWD16_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087792.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14753..15709
                     /locus_tag="IWD16_000710"
     CDS             14753..15709
                     /locus_tag="IWD16_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9087793.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(16014..16970)
                     /locus_tag="IWD16_000711"
     CDS             complement(16014..16970)
                     /locus_tag="IWD16_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP9087794.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            17074..17676
                     /locus_tag="IWD16_000712"
     CDS             17074..17676
                     /locus_tag="IWD16_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9087795.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17764..19263)
                     /locus_tag="IWD16_000713"
     CDS             complement(17764..19263)
                     /locus_tag="IWD16_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP9087796.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(19279..19803)
                     /locus_tag="IWD16_000714"
     CDS             complement(19279..19803)
                     /locus_tag="IWD16_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087797.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            20101..20850
                     /locus_tag="IWD16_000715"
     CDS             20101..20850
                     /locus_tag="IWD16_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP9087798.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20841..21626
                     /locus_tag="IWD16_000716"
     CDS             20841..21626
                     /locus_tag="IWD16_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9087799.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21595..22311
                     /locus_tag="IWD16_000717"
     CDS             21595..22311
                     /locus_tag="IWD16_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9087800.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(22413..23432)
                     /locus_tag="IWD16_000718"
     CDS             complement(22413..23432)
                     /locus_tag="IWD16_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP9087801.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23451..24374)
                     /locus_tag="IWD16_000719"
     CDS             complement(23451..24374)
                     /locus_tag="IWD16_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP9087802.1"
                     /translation="MLSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYII
                     LILVSKPFFLSYKGDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRI
                     FLYSYGYIKMSGLKMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEIS
                     QVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVK
                     VVFVPALNIVILAVLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLAS
                     NASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT         7243 a         5234 c         4274 g         7646 t
ORIGIN      
        1 tacttaatga tggtatatta gaattaacta aaaatttgga taatgggttc gaggaggcac
       61 ttgccgatgg aagatgcaat aaaacaaggt cagctttttt tatcagaatt aacagctgaa
      121 gaacgttcct gtctatataa tgatttgctg tcgatcaaag acgtttattt atgcaataaa
      181 gatactagtt tttatacatt ccgaaaaatc cgccgagcaa ccaagcgctg tgctgaatgc
      241 cgtaaaagga cttttcttta tcagacagaa cacaaaggcg aactttcaat cagtcagatt
      301 catgaacaga agcgtttcaa aaatcattat cgtatccaga tcttattcaa tggacaagag
      361 atggaatacg cttttctcga aaacttgctg caacatctcc ataaccagca ttttagttat
      421 aatagccaag ccatttacgc ttaattcctc tattttttgt atgtaaagag gcacggtaaa
      481 gaggtagccg cttttaaaat caggttccta ttattgataa gaaaatataa taaactaaaa
      541 gaagcagaac cgctaacgga tctgcttctt ttttggttat ttttgctatt ctattttaat
      601 tgaattttgc aactcactct tctttattcc aaacctactc gtttgaatat cacgtcaacg
      661 tttttcaaat gatgatgata atcaaaggca tcgtctattt cttctttaga taataccgat
      721 gtgatggttg gatcttcttc taacagcggg cggaactgca cttggttgtc ccaagcgtac
      781 gcagtttttg gctgaacaag atcataagct tcttctcttg tcattccctt atcgatcaat
      841 ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg cagacatatt ccgcttcatg
      901 ttttcaggaa agaccgttag atttttaacg atattgccaa aacgatttaa catgtaattc
      961 aataaaatcg tggtatcagg caagatgatt cgttctgctg aagaatgaga aatatctctt
     1021 tcatgccata atgtgacatt ttcataggca gtgaccatgt gtccgcgcac cactctggct
     1081 agaccagcca tattttctga accgatcgga ttccttttgt gcggcatagc agaagaacct
     1141 ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt cggatttttg caatccgcgt
     1201 atctctgttg caaaacgttc aatacttgta gcaataagcg ccatcgctga gaaatattca
     1261 gcatgtagat cccgagggag aacttgagta gagatttctt gtggacggat ccccaattgt
     1321 ttacatacat actcttcaat aaatggcggg atatttgcga acgttcccac tgctcctgag
     1381 atttttcctg cttctactcc ttttgccgca tgttcaaatc gttcgatgtt ccgtttcatt
     1441 tctgaatacc atgtggcaag tttcaatcca aaagtagttg gttcagcatg gacaccgtgt
     1501 gtccgaccca tcatcactgt atatttgtgt tcttttgctt tttcgccaat gatttccgta
     1561 aaacgtttca agtcttcccg taaaatatca tttgcttgct tgatgagata gccataagca
     1621 gtatctacga catccgtact tgttagacca taatggaccc attttctttc ttctcccaat
     1681 gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc gtgttaattt ttcgatttcc
     1741 aagatccgat cgatatcaaa tgatgcatgc tcacgaattt ttttcacatc ttcttgcggg
     1801 atttcaccaa gttctgccca agcttcatct gctaaaatct cgacttcgag ccacgcacgg
     1861 tatttgttct cttccgtcca gatattcccc atttcagggc gtgtataacg ttctaacatg
     1921 ttttttctcc tctaatccca aatattcgtt tgtttgattt cttccagcgt ttgctggatg
     1981 tcttctgtca agatagtgat atgtcccatc ttgcgacctt tttttgcttg tttttttcca
     2041 tagaaatgga attgccagtc tggcttattc tcaatcaagc gatagctttc cagcagttgt
     2101 tctcccaaaa cattgaccat tactgcattg cttatcaaac gtactttttc tcctaatggc
     2161 caaccacaaa tacctcgaat atgggcatcg aactgactca tagaacaagc ttcaatagaa
     2221 taatgtccag aattgtgagg acgaggagct aattcattca catatagccc gcctgttttt
     2281 gttaaaaaca tttctacccc taaaacacct gaaaggctca ctgcttctgc aatcacacgg
     2341 gcaatacgct cagcctcctc gatcacatcc tgatcaatcg cggctggagc aatcgtttca
     2401 tgaagaatat tgttgtggtg gatgttttcc acgaccggaa aagtcgtgaa atctccttga
     2461 ccatttcctg ccaccatgat cgaaatctct ttttcaaacg ggatccatgc ttctaaaaca
     2521 caggttcctt ctcttaaaag gtccatggca ggagctagat ccgcccgact tttcaaaaca
     2581 tactgcccct tgccatcata gccgcctcga gtggttttca aaacacaagg atagccgatc
     2641 ccgtcaatcg catcttggat atccgtcgga ctgacgatcg ttgcataagg agcaatcacg
     2701 atgttattcg tttccaaaaa agatttttct aacaggcggt cttgcgtaat cgcaagcaag
     2761 tctgtccctt gaggaataaa tgacatgggt aaaatcgcat ttaacgcctc tacactgacg
     2821 ttttcgaatt cataggtaat gacatctgtt cttctggcaa gctctttcag cgcaaaggtg
     2881 tcatcataga cactttcaat gtgccaatct gcaacttgtg cggctggaca atcgctcaca
     2941 ggatccagca cacctacttt aaagcccatt tcttttgcac taatcgccat catacgacct
     3001 aattgaccgc caccgacgat cccgatcgtt gaaccaggca acaaaggttt aaccaagttg
     3061 atcactactt tctataacag ttttagccaa cattgaacgt ctttcagcca atttagcctc
     3121 caattctaaa tcatacatgg aaagcatctg tatcgcaagt agtccagcat taattgctcc
     3181 cgcttttccg attgctgtag tagctacagg tactcctcca ggcatttgga caatcgaaag
     3241 aagcgaatcc aaaccattta atgttcgtga ttggaccggt acaccgataa ccggcaaagt
     3301 tgtttttgat gcgaccatac ccggcagatg cgcggctccc cctgctcctg caataatgat
     3361 tttgattcct ttttttcgag cgttttgtgc atattcaaac atcaaatctg gggttcgatg
     3421 agcagaaacg accttttttt cataagacac accgaattca tccaatattt gacatgcttc
     3481 tttcattgtt tcccaatctg aagtactccc cataatcaca gagatttttg ctgtcattgt
     3541 ttttcctccc agtttttcta cattcatatt ttaacaatca ctttttttga tgtcaaagaa
     3601 atttcgaatg ttaacaaaga aaaacatttt attgttcgtg tttattaaag atgaaaaaaa
     3661 ggaatgtgac aaaagttagg tcacattcct ttttatggat aaacggtgtt caatcagctg
     3721 accctcggta ttagctcaaa caaacggcaa aatacgagga actatttttc cgtttgtttc
     3781 tctaacaatc aggtcaaaac gctgatttca caacctttct tttgctctat tacttcttga
     3841 aggcttcttc caatatttct tcatcttttt tcaatccatt aaagatcaaa ttcaatatga
     3901 tagccgtaat gctactcatt acgatgccat ttccagtgaa catttgcaat gtttgcggca
     3961 tttgactgaa taatgtcggc gtaacattga aacccagccc aaatccaaca gaaacagcaa
     4021 caatcaatag atttttatca ttgctgaaat caacttccaa cagcatacgg attccttgga
     4081 ctgccaccat cccgaacatg actaacattc ctccaccaag taccggctca gggatgattt
     4141 gtgctaacgc accgatttta gggaataatc ctaaaacaat caagaagaat gcagagaaat
     4201 agatgggttt tcttgttttg attcccgaaa gttgaaccaa tccgacattt tgagaaaagc
     4261 cagtataagg gaatgtattg aaaataccgc ctaagataac ggcaagtcct tctgcacgat
     4321 atcccttttt caattcttct tctccaacat ttttcccagt aatgtcacca agtgcaaaat
     4381 atacacctgt tgattctacc atgctgacca tggaaatgat gatcattagt aaaatcgacc
     4441 atacgtcgaa agttggttta ccaaaataaa agggttgtgg aacatggaac aacggcgcat
     4501 ctccaatcgc gctcagatca atcattccca tgaaagcagc taagatgctt cctccgacta
     4561 aaccaatcaa taccgcgatt gaacgaatga acccttttgc cagcatttgc acgcctacaa
     4621 ttaggagaat cgtcacaaat gcaagtagca gatttgtttt gtcaccaaaa tcagctgctg
     4681 ttgcattacc tccacccatt ttctcgattg ctacaggaat caaagtcaaa ccaataacgg
     4741 tgataaccgt tcctgttact ataggaggga atagtttttt aattttagaa aagacaccag
     4801 aaactaacac aacaaagatc ccagaagcga taatagatcc gtagatagct cctactcctt
     4861 cattggttcc gatcaagatc agtggagcta ctgcttggat cgcacatcct aaaacgacag
     4921 gcaagccaat tccgaaaaat cggttaactg tcaattgcaa taatgtcgct aatccacaca
     4981 taaagatatc aattgaaatc aaataagtca tctgttcttg attaaagcct agtcctgtac
     5041 cgatcaaaag aggcaccgct actgctcctg catacatcgc tagcagatgc tgcagcccta
     5101 aaaccgcagc ttttccattt tgtgtttctt tttccacgat tatgcgtcct cctcaagaaa
     5161 ctcaacttgt ccattcgtca aggaagcaat acgcgcaaga gaaacgactc tcaatcccat
     5221 ttcttcaagt aactgacgtc catcttggaa agacttttcg atgacgatcc cgattccttc
     5281 tacttgcgct ccagcttgct ggcataattc gatcaatcct tttgccgctt gtccgtttgc
     5341 caagaaatcg tcgataatca atactttgtc ttctgaagat aaaaatttac gagaaataga
     5401 aatcgtactt gtcacttgtt tcgtaaaaga ataaacagaa gctgtcaata attcttcatc
     5461 catcgtcaaa ctttttgctt ttctagcgaa gatcattgga acatctaatt ctcttgcagc
     5521 aaataaagct ggtgcgatcc cagaagcttc aatggtcaca actttcgtga ttccttgttc
     5581 tttaaatact tctgcaaacc gttttcccat ttgttccatc aatacagggt ctacttggtg
     5641 agtgacgaag ctgtcgactt ttaatacgcc ctcgcctaat atacgtccgt ctttttgaat
     5701 acgttcaact aattctttca ctttatttgc tcctttatca tttattaata ccgaaataac
     5761 cgcttttgtg caaaaaccta ctatgtttct gttaaagaaa aacaccctcc actttatcat
     5821 aagaaaagaa gggtgaaata gccaatcttt tacttatagt ccagcattta cggtactggc
     5881 tagaaactgt catccatatt atgacgatat ataagtaggt atgtaaaact aataatagtg
     5941 taaattcatt ttcttgcaac tgttttgcac gtgaaggcag tttgccatat ttgaataaaa
     6001 aatgttgttt caaatattga aaacagtcta acactacttt ctgaaactga caacgtggtt
     6061 tacttatttt tttgtaaaat ctaatgattt ttaatgcatt attccttttt gttcgggttt
     6121 aaatagttat ttttattttc tcctatctaa aaaagcagcc cacataaaaa gaaggcatga
     6181 caaaaagcat gtcatagccc ctttttccga ataaatagtg ttcaaaagac agcctatcgg
     6241 caataaggca agattttata aactacggga agtaatttcc gtaaatctat tcttattctt
     6301 ttaagctgac aagctcgtca atcactactg cttttcctga agcaatagaa tgatttgctg
     6361 cgattcctgt caaaatcgac aaaataccat ctacgtgagt tgctgcccga ttccacggat
     6421 cttcttcttt ttcttcacca aatagatcat ctaataaacg cggatctcct ccgccatgtc
     6481 cgcccgcttc ttgttcgacg attacctcat atggctcacc gaacatcgga tagacatgga
     6541 tggactgaag aacggctgct ccttcatcct ttttatctcc acctgcattg atgtaagact
     6601 tttcgatcac tttcatttcg atccgtcctt tgctgccatt gaaagaaaca ttgaatcctt
     6661 cccacggcat atacgcgttt agcgaatacg tcaaaatcgc accagtttca tatttgaccg
     6721 ttacgcctag tgtatcttca atagagatat catcggaaaa tacactgcgg tcacgaatat
     6781 atccactgtc tttttctgct tctaaataca tttctcgcaa ttcctcattg gctgacaggt
     6841 ctatagcaaa tggatcagct ttagctagtt cgcttccata gcatcgactg taaaatattt
     6901 ctactccgcg tttctccgca ttatgcatcc cataaaagtt taatccaccg aatgcaaata
     6961 ccttttgggg acggctgttc aaccaaaaat tcactagatc aaaatgatgg gtagatttat
     7021 gaacaagaag cccgccgcta ttgtgcttat ttcgatgcca tcttctgaaa taatctgccc
     7081 cgtgttgcgt atttaacagc cattcgaagt ggatagaaaa cacatcacca atcacatcac
     7141 tttctatcag ttcctttatc ttcgtgtgat acggtgcata acgataatta aaagtgactc
     7201 gcaggcttct tcccgtctgt ttttggaccg ttattatttc ttgagctttt tcctcatcga
     7261 tagtcagagg tttttcgcaa ataacatcat atcctttttt cattgcacga atgatataat
     7321 catgatgcgt tcgatctact gacgtaataa tgacttcagc cggccgtacg tcatccagta
     7381 gttgctcaaa ttgtgatggc atacatgtct ttactttttt ctgtccatac ttgttcacaa
     7441 gtatccgatt tgcataatcc atccttgttt gagatacatc acaaaaaccg actagttcac
     7501 aagtttgatg atacgtagag gcaattgcct cataaaaaaa acgcgctctc ccaccaagtc
     7561 cgacttgtac atattttttc ttcatttaaa gtcattctcc ttttctttta agcaaaatac
     7621 tgtgacaaat ggcagccctg gatacagtaa tgcagtgagt aaaaaatcag gctgtataca
     7681 ccagaacagt ccatacaaaa ggcataaata ccacagaaac cccttgacgt tttttgacaa
     7741 cagccagata ttccttttga tcaaataaac aagactatct gctttatctt ctttgcagat
     7801 atcaattagc gtactaaaaa aactatggga tacaaaaagc aacagacaca atagaaaagg
     7861 aatcagatac caagctccaa tctttcccac taataaatac caatcaactg caaaaataat
     7921 gacaaacagc tgaaacaacg taatgatgaa acacatccgt ttgattttcc ctttcctcca
     7981 acataccttt ttttctgcca agtaatggac agatagataa taagaaggat acatcatcaa
     8041 aaaataaatc agatagacaa cacgagcaga cagcactaat cctaaagtga acaaacatcc
     8101 tagaactaaa gcgaaaagag cattactgat aagactggtt aaaaaaatcc gacagacata
     8161 gtagttgagc ttgtaaagaa acatctcttc tattggcttt ttcatattca gcccttcaga
     8221 ccgcttgtac tgatcccctg gacaagatat ttattgaaga ataagaaaat cagaaaaacc
     8281 ggtagcagcg acaacgtaga catcgcaaac atactcgcgt aagtcgtact agatccagcg
     8341 tctgcaaata atttgatggc aatcgatacc gtgtaagatc tcggcttgtt taaataaatc
     8401 aatggtccca tgaagtcgtc ccatttccaa taaaactgga tgatgatcgt cgtgatgatc
     8461 gaaggtttca gcaaaggaaa aataattttg gtaaagacac tatacttgct gcatccgtca
     8521 ataatcgctg attcatctaa ctctttgggg atgctgacca tgaattgcat gatctggtaa
     8581 atgaaaaatg cttgtccaaa aaaatgtggt aaaatcagcg gcacatacgt cccaaccaaa
     8641 ttcagccgat tgtaaataat aaactgcgga atcaaaatca cttgtgcagg aatcatcatc
     8701 gttactaaca tgacgacgaa ccagaatctt tttcctttga aatcgattct cgataatgcg
     8761 taagcaatac aagtcgagct cagaactgtc cctatcgtcg aaactgcaga gatgacaaac
     8821 gaattcctaa agaaagtaaa aaatgaaatt ccagcaaacc ccgcccatcc ttttgcgtaa
     8881 ttttcccatt gaaaagtctc tggaatcaaa ttcagcgaac cactcataat ctcttgatcc
     8941 gttttaaaag aacctgcgat catccataag accggataaa tcatgactag acctaatagc
     9001 aaaataaaac cgtattgaaa ccatttcgtt actgtattat tcatttcttc ctcctctcat
     9061 tcacacaacg acccattttt ttgatgattt gaaaatcaga aaggtgacag ctcccataat
     9121 caacagcatg atccacgaaa tagcactagc ataccccata gaattaaaag aaaaagcttg
     9181 gttatacaca taaagagcat aaaagttcgt ggcatcattc ggaccacctt gggtaatgat
     9241 aaatgcctgc gtaaaagtca taaacgccgc gattgtctgc ataaccaagt tatacaaaat
     9301 gatcggcgaa agaagaggta acgtgatctt gtaaaacgtc tgccatttgt tcgctccatc
     9361 gatctctgcc gcttcataat acgagcctgg gatctctttg atacctgcag caaagatgat
     9421 cattgccgaa ccgaattgcc aagcagacat caaaacaaga gggatcatga ccagtttttg
     9481 atcgctaaac caggaaagcg caggtgcgcc taatcgttcc aggaaaatat tcactaaccc
     9541 gtttttagaa aacaactccc gccaaactag agcgatagaa atactgccac cgatcaaact
     9601 aggcacataa tacaaagaac gaaagaaagt cgttcctttg atattcctcg ttaaaagata
     9661 agcaatcaat agaccaaaga tcagtttcac aggtacagat gcaaatacat agagaaaagt
     9721 gactcggatg gagttcaaaa aacgggcatc actcagcatc tttatataat tatccaagcc
     9781 gatccatgag actggatttc ccaaacgata atcggtgaac gaataataaa gtgacaagat
     9841 catcggtatc actgttatca atagaaatcc taggatgaat ggagaagcaa atacgtaacc
     9901 gaccacattg tcttgatcca agaaccgttt tagtttttct tttttcatgc cttccttctg
     9961 ctgttacttc ttcataaatg actttttcaa ctaaaagatt gtattggtct tgaatttccg
    10021 gattcaaagc actatcaaag acattcactt cgccgttgtc catatcggaa atcatttcta
    10081 aataatcaaa tgtcgcttgc aaagttgcat catctgcacg agcgagctgt tctcgaatat
    10141 tcgacatgat tggtacacca cgttctcctt ttaaaatttc attggcttct tcactgtttt
    10201 gaaagaaatc aataaactta gcagcttctt ctttatgggc tgagcttttt gccatactca
    10261 gcatttgcga agaacgtaaa gggataccag attctccatc tgcttttctg cgaggtggat
    10321 taatcaactg aatctccata tcttcaggag cccctgacaa tatttctgtc aactggttac
    10381 ttgatagaaa caccattgcc gcttctctat ccctaaccgc ctgaaaagac agatccgtcg
    10441 tacttgcagc tccaccacct ggttctggat gggccccttt gtctaccaga tttttacgca
    10501 gctggaaaaa atctgttaga tagtctggat tttcaaatcc caagctgtta gatagatctt
    10561 cccgattata aaaattcaat cctttctcaa cttgtggtat ttgaataata cagccagcta
    10621 taaaatcttc gaatcttgaa atagcataga ttcctaattc ttcagtgatt tgatagctga
    10681 tttgttcaaa ttcatcccat gtccaatttg aagctggttc agggatattt aattgttcaa
    10741 atatattttt attgtaagca atcgcaaacg aattcacccc gttagacagt ccaactaatt
    10801 gcccgtcgta agtcgtcgtt gctaaatagg attcatctgc attagaaata tcaattatcc
    10861 cttgctccac atattcatct aatggttcaa tctgattgat gtattgagga aattgattgt
    10921 tcagttggaa aatgtcccag atatcatctg atgctgctag tgtacctaat tttgtgtaat
    10981 atccttcata atcataaaat tcataatcga tcgtcacatg gggatttttt tcttcataaa
    11041 gctcgattac ttttactgtt gcatcgtgtc ggacttgcga accccaccaa gccatgttca
    11101 gcgtgatttt tttgtcatct tcttgcccac aagccgtcaa gaagaataac ataacactta
    11161 aagctaagat gattctcatt cttttcttca ttctctgcac ctctacttcc attgttattt
    11221 ttcaatatac tgtattttta tacagaacat atttattaaa cgatcttttt caaggggaat
    11281 aaaaaccaag acttgcctta tcgttctgcc taggatgaac aactcaatac tttcaagaaa
    11341 aattagatag tgctgattct tctttcatcg ctaaaaacac ttcttgcttc aatgatggga
    11401 ggataattac gcaaagaaaa aaggatatca gacaactgcc caatatcctt gacatagcta
    11461 aataagcatg cttcttcgtg tgcgctcttc cctaaaaaac agaaaaataa ctagtaattc
    11521 tgtatgaaat taggtacaat atacacgtcg atgcacaagc attgactatg gcaacttgat
    11581 tggtcagttc ccgctgactt ttcaagcccc gacaatttag ctgctactaa attgtcgggt
    11641 gcctttttta gtttttattc gtttttcatt ctatagaaag cgcttcaata aatcaagaca
    11701 tttttttgtt agaatgctaa taataacaaa aggttttaac tcttcttatg tacattacag
    11761 gtaaaaaaac aaaaaaattt ctcttttttt tgaattctga acaaatagta ctggagtaca
    11821 aaagacgagg aagagccact atctgctctt cctcgtcttt tgattctcac ctgttattca
    11881 tgattctttt tctcagtttc ccgatatttt tccattttac gactaaaaag ctctccgcta
    11941 cttggtggcg tatatgtgat ggcatttgct cctgcttgaa tggtctcagc aatactttct
    12001 tccgttggac cacctgtcgc aatgatagga agttcaggat attttttacg aaaatggcgt
    12061 actgcttctg cagtttcttt acctgcactg atattgataa tcgaaatacc agctgctaac
    12121 ttttcatcta tttctgtata tttcgacgtt accgtactga taactggaat atcaactact
    12181 cgacaaactt cttttactgt ctctaaaggc gtaggaccgt tcaaaacgac ccctaacgaa
    12241 ccttgcgctt ctgcgaacat gctcatgtag gaagaacgag caccttgtgt caaaccacca
    12301 cctacaccgg agaaaactgg aatgtcagct gcctcaataa tgcttttagt aatcgctgga
    12361 tgaggtgtaa aaggatacac agcaattact gcattcgcat ttgtattacg tatgatcgca
    12421 atatccgtag taaagatgat cgaacggatc aattttccaa atatcttgat cccgcttgct
    12481 tctaaaatga tttccggtac tcttacgata tcttttctca attccgtcat gatttctggc
    12541 gtaaattgtt ctgccatcag ccacttctcc tttgctatct ccggtcatac tgattcaatt
    12601 gatacgtctc gatgacctgg atgatttttt gcgcataacc ggggtctgtc gcataacctg
    12661 catcttgcaa tgcttgtgcg gcttcctgat agttggtggc cgtaatcact ttttcataca
    12721 gcgcaggatt ccaatctact ccttggacga acagttgtgt atggtcatcc atggactgtt
    12781 cccatgaatc atacactttg aagtctcctt ggatagttat ccattcttcg ttaacaaatt
    12841 cctttgtttc caggcttact ttcttctgat ccccataagc cttgatcccg aaaagattat
    12901 tgtatttgct agccaaggta ctttttcccc aatttgattc taatatagct tgtcctaaaa
    12961 tgatactagg aagcactcca taaccatctt gtagttcttg ggcatgcgga acaagccgtt
    13021 caataaattg ctgatgggat tgcgcttcat tttcttgctg attatcagta aaaggagaag
    13081 acagattctt caaggaaaaa acaaaggcta agagtatcac tgctacaccg gcaaccactg
    13141 ctggccaacg gatattcttg cgcttttgct ttttccactt ttttttcgcc atgatcaatt
    13201 cctacttttc atttaattca ttcagatatt cttctagcgt caattggtgt ttggtgatat
    13261 actcagcgtt ttctttaccg acataacgta agtgccaagg ttcatatgtg atcttagtta
    13321 tatcttcgtg tccttttgga taacgaacga taaatccata ctgcgcagca ttatctgcga
    13381 tccatttagc tccaggctga tctccgtaac ttgcatccaa tacagtagaa ggataactgt
    13441 tgtaccagtt ttgatcgacg acatcgactg ccagacctgt gtggtgttcg ctgtatcctg
    13501 gctccgtaat cgtttcttta gtaatctttg tcgcctcctc ttctgataca ccttgacgac
    13561 tcatcacatc ttggacattt tgttcgaaaa cttgttgttg ggatgataca gaccggtaag
    13621 ctgagaccat cactaatgga aatccagctg cttcagcagc cttggcaaat gcttcatatt
    13681 ctggagcaat gcgtttgtcg atcaagtatc cgttacttaa tgatgccaat tgagtagact
    13741 catcaatttc tttttctaat ttgtgatctg gaccaaccaa aactagcttc caatcgtctg
    13801 ttttaacatc tggcaattgt ttggacgtac gatcttttgt ttctttgtcc tttaccttgc
    13861 tgctggttgt ttgactgttc ttttctttac tagcagttgt tttggtgctt gttcgttcga
    13921 ctgcttgatt gccggcttga acatgcgaat catcatcgct aaaaataaca tagcccattg
    13981 ctgcgatcat aatcacaact gctccaaatc ctaaaaattt attcacttga aaaatccttt
    14041 cgttgttctt cacgttcccc attcgcctca tgaacgtaag tttcttccag attttcattc
    14101 acccaatcga gaagttcgat tttttctcct tcgatctgtt tttgaacatc tgctggcaga
    14161 cgaaaattca aaatatcatc atagccccat tcatcatatt gaatacttac tttcaagtca
    14221 aggtatctac cagcctgctg attatttttc aaagggatca gctcgacttt tgctgctttg
    14281 ctgctaagcc atttttgcgc gatgcgtgtt ttgatttgct tgtactctgc aacggcaacg
    14341 tttcgttttt cgggaaaaat aatcgtttga cgcaaaatct tttgcgttag catccattca
    14401 taatcagtga aaagattttt tactttctgc atcgcttcag atggcagatt tttctcgcca
    14461 tctttccatt tttgccattg ttctggactg atcagcaagt attcatctaa aaaatttttt
    14521 tcagtttcaa actgttctaa taatacactc ataataagat caatatatac ttgatgcacg
    14581 tttatcccct ccttttaact attctactta agttttgatc aaaaagcacc tcttaaatcg
    14641 ctgctttcat tttttttaat gtttcaagtc ctctcctctt tattcttcgt gatttttgtt
    14701 ataataatag acagacaaga aactcaatcc aactatgaag gagcgttttc agatgaaaaa
    14761 atatcaatgg ggaatcgttg gtcttggtac catcgcacat gagtttgccg aaagcttcaa
    14821 tcaagagacg agcgaactaa cagcagttgc ttccagaact tcagaaaaag cggaaaactt
    14881 cgctcaccgt tacaatattc caaaagctta cggaagctat caagaaatgt tagacgatgc
    14941 tgaaatcgac atcgtgtaca ttgctgtacc aaatagacag catatcgacc atatcttggc
    15001 agcattggaa gcaggcaaac acgttctatg tgaaaaagcc atcacgatga ataaaaaaga
    15061 actggctgac gctatgcgct tagcagaaga aaagaatttg atcttagcag aagcgatgac
    15121 aatcttcaac atgccacttt atcagcagtt acgctcaata atggatacag gaaaactggg
    15181 ggctttaaaa atgatccaag cacctttcgg cagttacaaa gaaccagacc ctaaaaaccg
    15241 gttcttcaat ccagaattag ctggaggagc tctgttggat atcggtacgt atgctgtatc
    15301 tttcgctcgt ttctttttga gctctcaacc tgaagtagtt gcctctacaa tggttccttt
    15361 tgaaacaggc gtggacgaac aatccgtcac tatcttgcgt aacaaagaaa atgaacttgc
    15421 tgctgtcagc ttgacttttc aggcaaaaat gccaaaagtc ggtatcgttg cctttgagaa
    15481 cggttatgtg acgattgctg attacccgcg tgctgatcgt gcggagatct tatttacaga
    15541 tggtacaaag gaattcatcg aaagcgggaa tacatctgaa gcaatgaatt acgaaataaa
    15601 gaatatggta cacatgattg aaggtgactt gcccaatcgc tctctcttct tgacaaaaga
    15661 tgtcatcgaa atcttggatc aaatgcaagt tctatggaaa aatatgtaat tttaaaaggt
    15721 tgtgaaaaag ctgttctgca tcaagtaata agaacagcca aacaaaaata gctcctccta
    15781 tttttcgttt ggctggactt attaccgcag aggaaagctt ttgaacaccg tttaagaaag
    15841 aggttgtgag cctaccgttc agctccgagt cacaaggagc aatttcaaaa aacagcttct
    15901 catgtttgtt gaatattgtg acttgtgacc gaggagttga caggagaaca ccgtttaaag
    15961 aagaggttgt gaaatcagct ttttgacctg aatattagct gaattcacaa cctctatttt
    16021 tggatttttt gttgggagaa ttctgccttg atttcaataa aacttttaaa agactgcaaa
    16081 aactggaaca attctgctcg atcctcgaat tcagggcgac tagtcggcag aggcatttgc
    16141 cgatacaata cataaacttc ttcaatacgt gttaaaattt gtctgccatc atttgactct
    16201 gaaaatgttt ctgcagtata aatcaataaa ccatagatat gttttccata ataagctggt
    16261 tgctggattc tctctagatt ttccagcatg tctttaatca cattggcctg tgctcttctc
    16321 atagagaagt aagtttcgta gtatagcggc tgtctgagcc agtagttttc ttgatgttct
    16381 ctggcatctt cttggctttc gcgaatgaat gtcagtaaat gttcgcattt gatctgcaca
    16441 tcttctttat tttctgataa aagaaattct gccatttgtc tcaaaatatt tcgaaattct
    16501 ttttcgatct gtatttggtt ttccttcagc tgcttcgtac gatctggcat gtaaacattt
    16561 gcaagcaaag caaatactaa tccaatcgcc attaatcccg cttcattgcc gatcagctgc
    16621 caagacatgt tttcctccac taaataatga gtgaccaaaa ccgaatttac tactatccct
    16681 tcggttaatt tgaagcgaga ggcaagcggg atgaacagga ataaaaaaag actgaagcca
    16741 aaaatagtat acccaacagc aaaaaaactg aacagtgctg ttgccgtagc taatataaag
    16801 gctgcgattc gattcatgcc cgtacgcaat gtagagcgct tggtatttcc tacacttaat
    16861 aatgcaatga tccctgcagc tggccaatac aacagcgata agctacccgc tgtgaccatc
    16921 gaaaagaaga cactgatcac tgttttgact gttcgcattc ctatacgcat ttacttcccc
    16981 cttttttatt ttaattatac ttgaataatg aaaaactagc tatcagacaa aagtttaggt
    17041 atgatagtaa aaagaccaaa aaggagaatc tctatgcaat ttggccgttt tcgactaggt
    17101 atgcgtacga tcaaaagtgc cttagctgtt tttttatgta tcttgttttt tcatttgact
    17161 caacgaggaa tcccgatgat tgctgcatta tctgctgttt tttctttgcg tcaggacttg
    17221 actacaaccg tttcttttgg aaagtctagg atcatcggca atacattagg tggtacatta
    17281 gctatcattt atttttttgt caaaagctat tttcacaatg attttttggt agaattgatc
    17341 ttgcttccta ttctagttat tattgtgatc gtcgtgtcag atggtattaa caataattcc
    17401 ggtatcattt ccgcaatagc tacgttgttg ctgattgcgt tgagcatccc tcaaggcgaa
    17461 tccgctttgt atgctatcca acgtgtacta gatactttta tagggacatt cattgcaatc
    17521 ggtatcaatt tctttcttcg tcctcctgaa acagaaaaga aggaagaact atcagaagat
    17581 ttggtcgaac tccaaaagaa agaacgattg ctaagaacaa atttacaaga gatagaagct
    17641 aagatccaaa aacaaaagaa aaatagcgag gactaatttt cttttgcaat aaaataaaaa
    17701 aatggagaga agaactttaa aaagtcttcc tccatttttg attgaacggt gaagtgtact
    17761 actttattct tccttcatcg agttttgttc tgcctctttt ggcgcttcat acatatttac
    17821 ttggctagta gtcccacgtg tcgacaaatt ctctacgatt tccttcaagt caagtcctgt
    17881 tgtcgctttt agtgtttctt gagtagaaga aagcaaattt gtggcataat ttgtcactcg
    17941 gttcgctcca ctggtttctc ctgaactgct tcctgtatcg acaacagaga ttttttcgat
    18001 atttcctagc ggctgtgctg cttcgcgcat caattgcggt agcatatcga taaccatact
    18061 caagacagct gcttcgccat attccttgaa ggcattggcg atcttttgtt ttgcttcggc
    18121 ttctgctttt ccttttgcta gtgctgcttc ggcttctgct tgtccagcta aacgtgtctt
    18181 attggcttcc gcttctgcca gtgcttccac acggaatcgt tctgcttcgg cttcagtcac
    18241 ttctttgact ttttgagctt gtgcttcttg ttctttagcg taccgatcgg catctgcttt
    18301 tttcttcact tccgaatcat attgcttttc acgacgtgtg atttcttttt cttccagttc
    18361 gatttgtttt tggcgttcta cgattttaat ctgcatttcc tgttcgatta cttgttgttg
    18421 tgctcgggca ctttctaaat tgtaggcttg gtctgctttt gctttagcaa catcttgttc
    18481 ttgtttgtag gttgctaatt tcagttcttt ttcttttaat gattctgcga tctctgtttg
    18541 tcgctgtaat tcagctgctt gtgattcttt ttctgcttga gcttttttga tacgcgtttc
    18601 ttttagagct tccgcttctg ctatatctgc atctcttttg acttgggcga ttcttggttt
    18661 ccctaaagag tcaagataac cgtttttgtc tctgacctct ttgatagtga aagaaacgat
    18721 gatcaacccc atttttgcaa ggtctacaga agcgacttct tgtacgcttt gactgaactt
    18781 atcgcgattt tgatagattt cttctacagt catagaacca agaatcgagc gcaagtgtcc
    18841 ttctaatact tctcttgctt cattttccag ttcttctctt gtcttgccta aaaactgttc
    18901 tgctgctgta gcaatctctt ctacagatga accgattttg atgatcgaag taccatcaca
    18961 catcaccggt acgccttgtt cggtatacac ttcaggggtg gagacgtcca atttactcga
    19021 taatagactg atccgattcg aacgttgaaa aactggcaag acgaatgcgc cacctccacg
    19081 cacaattttg attttgttat tgctttcatc aatgtgcaca tttttgcttc ctaaataact
    19141 tccactgatg atcaacgctt catccggttt tgctgtctgg tacttcgcta caaagacgat
    19201 cagcaacatc agtaaaatca aagctgcgat tgcgatcgcg ataaaaacaa ctggtatttc
    19261 catttaacac acgctccttt aagcttttat catgctttca taaggcatga cataacagat
    19321 tctttccttg atctcaatga tcaatacttt cgttccccgc tcagctgtct gttcttgggg
    19381 gctgtaaaaa caagctggac gactaatgga accggtaaca cttttcaact gaatttctcc
    19441 catacctcgt acagggatcg gtgtgatcac aacagctact tgtccttcca gagttttttc
    19501 agaagacgac atcgttgatt cggcatgttt catcggcatc aatatataga aattaagcaa
    19561 aaataccaac actatcgata tgcctccact agcaataaat aaaaataaag atgattgatc
    19621 tgttaaacgc tcacctagaa aaccaaataa gcttgtgaaa gttaaccaag ggataataag
    19681 catcggatca agcgggccat caaaatcgaa tacatctcca aaaataatca ataggactgc
    19741 caatcctgca caaatcagca atgtatacca ataaatcgtc tcaagtgcga ttccccctat
    19801 catatacacg cctccttcca tttctacgtc tatcatacca atgtttgaaa attctgtata
    19861 gcttctttta ccgactcagt ggtgtttttt ataggaaggt tgtgtttatc gttgacaatt
    19921 tgttaaattt ttgttttcat gttaaaattt tcacaataaa ttctttcgag gtagcctatg
    19981 tttccttttt ttaagaaaaa accattaaag ctccagaaca cgatcatcct tgtcgtttgt
    20041 ttctccacat tcgtttcttt gattgtttct gccatattga tcaggaattt tattctttcg
    20101 atgaaccgat ctagtgtgtc agataaaatc tctagcattg ctaaaatcgt tgccaatgat
    20161 aaactcatcc aaaaaaagct gcagactggt gacccgacac atgcgattca aggaataaca
    20221 aatgaaatca tgattgcatc gaatttagat tttatcgttg tcatggaccc tgaaggcatt
    20281 cgatattctc acgcagaccc aagttacatc ggtcttcctt tctcaagtca tgaagatgtg
    20341 aaaaaagtga ttggaggaca agaatttttc tctgaaaaag aaggaatgtt agggttcggc
    20401 ttccgctatt tcgtccctat ttattatgat caagagatta ttggtgcagt ttgtgttggc
    20461 ttaacaaaca acacgattga caatcaagtc aaagatggac aaaaaatgat tgttttttca
    20521 ttgattattg gacttagcgt aggcatcata ggcgccgttt ttcttgccca aaaaatgaaa
    20581 aaagtactgc ttggacttga accacaagaa atagcagaac agttaagtga aaaaacgatt
    20641 atcgaaaatg aagtagatga aggaatctta gccatctctt ctgataaaac tatcctcttg
    20701 atcaatacac ctgcaaaaaa cattttctac caaacagagc agctattgga tttcgaagta
    20761 ggaaaaaaat cagtgataat ctatatgacg ttttgtttaa agagacgatt gagcatcaaa
    20821 cgaccgttcg gactcaatcc atgtatttaa atgggataga aactatttct agcgttacac
    20881 ctctttttag caatgaccgt tttttaggag cagtcgcaac tttacgaaat cgatcagaag
    20941 taatgaaatt aatggaggag cttgggggga cgaaacaata tataaactcc atgagagctc
    21001 aaacccatga attcatgaat aaacttcatg tcatttctgg tttgattgat ttgaagaagt
    21061 atgaagaagt cacccatttt atccagcagc taaatcaaaa ttataaaaaa gagattggcc
    21121 aagtctcaag tctgatcaaa gtacctgcaa ttgccggttt tttattaggc aaaataaacg
    21181 aagccaaaga acaatctgtt gaaatgctgt tagaaagcga ttcttttatc cctaaaatgg
    21241 agatggatga ctccatccat tcacttttgc aagtgttagg gaacttactt gataatgcaa
    21301 aagaagccgt gctacaaaaa catgaaagaa acggacaggt ccggattcat ttaaactatg
    21361 acacagaagc tgaagtattc atcgtgaccg tctgcgatgg cggtgtcggg atatcagaag
    21421 aaatctcaga aaaaatattt acgctaggtt tttcgacgaa gggaatcaat cgtgggtttg
    21481 gattgaatct catccattca atcgtcaaac gccacggtgg atttttagaa ttttccaaac
    21541 aaacaaatgg cggtacaatc gtctatctag aattaccaaa aaaggaagat caaaatgatg
    21601 acaaatgtcc tgataatcga agatgatcca atggttgcag aattgaatcg tacttttgtg
    21661 gaaagcatcc ctaatatgaa cgtaatcgaa agtttcagaa atgtagaaga aggaaaaaaa
    21721 tatttcagac aaaaaaacgc agatctgctg ctgcttgatg tatacatgcc tggcgaaagt
    21781 ggtatccatt ttttatcttg gcttagacag caaggaaatg atatcccagt gattttgatc
    21841 actgctgcag atgatatcga aaccataaag aatgtgatcc gctacggtgc cttagattat
    21901 ttgatcaaac cgttcacttt gcaacgtttt cagctggcga tcgaaaagta tcttgctttc
    21961 tctcagacaa tccaaacgac tgaaaaagcc gatcagaaat tgatcgatca agtattcaac
    22021 cacaccactc ccattcacga aataccatca ttcatcgatt tgcctaaagg tctgtcaaaa
    22081 ctgactttaa aaaaaataag tgcatttatt ctaaaagaag atactatttt ttcaacaaaa
    22141 gaactggctc aaaaaagctc actgtcacgt atatccgtca agaaatattt gaatttttta
    22201 gtagaacaag atatattgaa cgaaagaatc aaatatttgg aaatcggcca gccgctaacc
    22261 tgttatgaat tgacaaagaa tgggcggaaa aagctcacag attactgttg agtcatcgaa
    22321 ttatcccata caaaatagtc caagtataca gaagtaacaa ctgtttactt ggactatttt
    22381 taaacatttt attgacgaat atccatgttt tcttatctta attgacgggc tttaggaaga
    22441 gatacctcat ttttcgtttt tccagtttct attatttcac gaaagttctc gaaacttgtc
    22501 gaaatcatat ttgataatgc ctcatctgta tttgaaccta catgcggggt tacaagaact
    22561 ctagggtaca tttctactaa ttgctggata gcagggtctg gaatcgtctc ccatggcttg
    22621 aaggatcgga aaaaaacctc tgtttcattt gcaaaaacat cggttgcaaa accagctaaa
    22681 tgattgcttt tcaaagctct taaaatagct tcattatctt gaagctctcc acgggctgta
    22741 ttaatcaaga tgctaccttt tttcatttga gctaaaaatc cttcattgat catttgatcg
    22801 ttttcacctg ggatatgagg tacatgaata ctgacaatat cgcttttggc taataactct
    22861 tcaagcgagt ggaacgtcaa tactttttta gcagcttctg tctgatgaat atcgtagccg
    22921 atcactttgg ctcctagtcc cttaaacaaa acagcttccg tcaaccctat cctgcctgtt
    22981 cctatgatcc ctactgtgca atttctgatt tcacgactaa acatttgctc atccactcga
    23041 aaatctttga aagatgttcg gatcgtcgta tacgcagtat ttctcaaaag catcatggca
    23101 aaagtcaaag ataactctgc tatcgcattt ggcgaatacg atggtacacg ggctactgtc
    23161 ataccaaaat cagctgccgc ttgtagatcg atatgattga ctcccacagt ccgtgtaaaa
    23221 acataacgaa tcccatattc ctgcattttt gcaagattgg tctcatctgc tatacaattt
    23281 cccctcaaca atacagcatc atggtccttt gctgacacga tattgtcatg tgtcaacagt
    23341 tcttcataaa gactgagatc ataatggtaa atattcaatt tttcaaaaaa atctatctcg
    23401 ttcggacgaa caccgtaaca tgcgattctc atactttctt cctccatttc ttatgaaaat
    23461 aaacctatgc catgaatcat ttgtaataca acgatccata caggtgtcag taccacagca
    23521 aaaatagttg acagtaagga tgcattggag gctaaaagtg cctccctatc aaaactgata
    23581 gcataggctg ctgccactgt tgcagctggt gtagccatca tgataacgat tgttgccagt
    23641 gcttcaaaat tgactggtag aatattcgtt gccgtcaaaa ctgctagaat cacgatattc
    23701 aatgcaggta caaataccac tttgactaga ctgtaatacc atgaagtttt ttcagatgca
    23761 gcttttttga agcttacttc acctaatgtt gcgccgattg ctagccacgc caatggtgaa
    23821 cttaaaccag ctaaataatc catcggctga aatagccacg gtgcagtctg gtcaattcgc
    23881 aaaaacgcta cttggcttat ctctccttct gcacctgtaa ctgacacttg tggcagatac
    23941 ccttggaaca gccaaatgaa aaggccggca aaagtagcga taatgattgg attgacgaac
    24001 atcgctttga tatttttagc ttccatcttc aagccactca tttttatgta tccataagaa
    24061 tacaaaaaga ttcggtagcc aatattgaaa attgaggaat acatcacgcc gacaggacca
    24121 taaatcgcac tgacgatagg cgttccgaaa aaggtcgtag aaccaaagat cgtcaggaca
    24181 cgcaacgttg tttgcttatc ccctttatag cttaagaaaa aaggttttga aactagaata
    24241 agaataatat aaataactaa tccccaaatc aagacattga ttccttgcat cagtaattct
    24301 gtattgatat cttgcatgaa agctcgaaat gccagtgctg gcaaagccac tgttaagacg
    24361 actttcgata gaatttttcc aacagattca gaaaaaa
//
LOCUS       DACDMM010000022        24180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDMM010000022 DACDMM010000000
VERSION     DACDMM010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.022"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IWD16_000720"
     CDS             133..3660
                     /locus_tag="IWD16_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9087803.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IWD16_000721"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IWD16_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP9087804.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IWD16_000722"
     CDS             7448..7789
                     /locus_tag="IWD16_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9087805.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IWD16_000723"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IWD16_000723"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP9087806.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IWD16_000724"
     CDS             9146..11566
                     /locus_tag="IWD16_000724"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP9087807.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWD16_000725"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWD16_000725"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP9087808.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IWD16_000726"
     CDS             13000..13737
                     /locus_tag="IWD16_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9087809.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IWD16_000727"
     CDS             13751..14566
                     /locus_tag="IWD16_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9087810.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IWD16_000728"
     CDS             14579..15226
                     /locus_tag="IWD16_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9087811.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IWD16_000729"
     CDS             15242..15898
                     /locus_tag="IWD16_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9087812.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IWD16_000730"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IWD16_000730"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP9087813.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IWD16_000731"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IWD16_000731"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP9087814.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IWD16_000732"
     CDS             18240..18758
                     /locus_tag="IWD16_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9087815.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IWD16_000733"
     CDS             18865..19365
                     /locus_tag="IWD16_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9087816.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="IWD16_000734"
     CDS             complement(19645..20565)
                     /locus_tag="IWD16_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP9087817.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="IWD16_000735"
     CDS             20730..21743
                     /locus_tag="IWD16_000735"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP9087818.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IWD16_000736"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="IWD16_000736"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP9087819.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="IWD16_000737"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="IWD16_000737"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9087820.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="IWD16_000738"
     CDS             complement(23383..24144)
                     /locus_tag="IWD16_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9087821.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
BASE COUNT         7994 a         4223 c         5107 g         6856 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
//
LOCUS       DACDMM010000023        23874 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDMM010000023 DACDMM010000000
VERSION     DACDMM010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.023"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..488
                     /locus_tag="IWD16_000739"
     CDS             6..488
                     /locus_tag="IWD16_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP9087822.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            525..1535
                     /locus_tag="IWD16_000740"
     CDS             525..1535
                     /locus_tag="IWD16_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP9087823.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(1650..2363)
                     /locus_tag="IWD16_000741"
     CDS             complement(1650..2363)
                     /locus_tag="IWD16_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087824.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(2553..3143)
                     /locus_tag="IWD16_000742"
     CDS             complement(2553..3143)
                     /locus_tag="IWD16_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP9087825.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            3627..3854
                     /locus_tag="IWD16_000743"
     CDS             3627..3854
                     /locus_tag="IWD16_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP9087826.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            3870..5036
                     /locus_tag="IWD16_000744"
     CDS             3870..5036
                     /locus_tag="IWD16_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP9087827.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            5270..6313
                     /gene="hrcA"
                     /locus_tag="IWD16_000745"
     CDS             5270..6313
                     /gene="hrcA"
                     /locus_tag="IWD16_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP9087828.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            6407..6970
                     /gene="grpE"
                     /locus_tag="IWD16_000746"
     CDS             6407..6970
                     /gene="grpE"
                     /locus_tag="IWD16_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP9087829.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            7021..8850
                     /gene="dnaK"
                     /locus_tag="IWD16_000747"
     CDS             7021..8850
                     /gene="dnaK"
                     /locus_tag="IWD16_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP9087830.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            9001..10167
                     /gene="dnaJ"
                     /locus_tag="IWD16_000748"
     CDS             9001..10167
                     /gene="dnaJ"
                     /locus_tag="IWD16_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP9087831.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(10249..10914)
                     /locus_tag="IWD16_000749"
     CDS             complement(10249..10914)
                     /locus_tag="IWD16_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP9087832.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            10890..11063
                     /locus_tag="IWD16_000750"
     CDS             10890..11063
                     /locus_tag="IWD16_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9087833.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(11108..11845)
                     /locus_tag="IWD16_000751"
     CDS             complement(11108..11845)
                     /locus_tag="IWD16_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9087834.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(11847..13349)
                     /locus_tag="IWD16_000752"
     CDS             complement(11847..13349)
                     /locus_tag="IWD16_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9087835.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13608..15602
                     /gene="uvrB"
                     /locus_tag="IWD16_000753"
     CDS             13608..15602
                     /gene="uvrB"
                     /locus_tag="IWD16_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP9087836.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            15612..18428
                     /gene="uvrA"
                     /locus_tag="IWD16_000754"
     CDS             15612..18428
                     /gene="uvrA"
                     /locus_tag="IWD16_000754"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP9087837.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            18691..19956
                     /locus_tag="IWD16_000755"
     CDS             18691..19956
                     /locus_tag="IWD16_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP9087838.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            19960..20691
                     /locus_tag="IWD16_000756"
     CDS             19960..20691
                     /locus_tag="IWD16_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP9087839.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            20826..21710
                     /gene="rapZ"
                     /locus_tag="IWD16_000757"
     CDS             20826..21710
                     /gene="rapZ"
                     /locus_tag="IWD16_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP9087840.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            21707..22705
                     /locus_tag="IWD16_000758"
     CDS             21707..22705
                     /locus_tag="IWD16_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP9087841.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            22739..23674
                     /gene="whiA"
                     /locus_tag="IWD16_000759"
     CDS             22739..23674
                     /gene="whiA"
                     /locus_tag="IWD16_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP9087842.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
BASE COUNT         7890 a         4131 c         5125 g         6728 t
ORIGIN      
        1 taaaaatgat tgacttggca aagaaattct tacacgaaaa tatatatttt gagttgtatc
       61 atcatcgagc catttataca aatgaagatg ctttggtaat aaagaaagaa caaggattta
      121 caggtacaga gaccaaaagc ttattcttaa aagataaaaa agataataac tacgtatatc
      181 tgacttttac tacgaaacaa acggatttca aagcattagg gaagatcgtc ggtaaaaggc
      241 tttctattgt ttcatcagaa cgcatggaag aacaaacggg acaaaagcag ggcgctgttt
      301 ctccgtttgg ttatgaaaaa cagattccga ttattattga tgaagagctc ttatcaaatg
      361 aaaaacttgt ttttgcacca ggtagaccag atcaaacgat ggtcatccaa acggttgact
      421 taaacaaaat catccagcta cttgagatgc cgatattttt gatgccggca aacgaaaaaa
      481 acaattaacc aagtaaaata gtgcatactt ggaggtgaag caacatgata agttatccat
      541 tattagaaag aaatgcaacg attggggtta ctgcaccatc ctcaggcgta tcagaggaac
      601 accattcgtt actcgaatca gctttaaaga gaatggaaga aaaatctttc caagtagtta
      661 ctggaaagac gctttggacg caagataagg aaaaatcagg tccagcaaga aaacgagcag
      721 atgaattcaa tcaaatgatg caagatgaaa atatccaaat gatctttccg acttggggag
      781 gagaattatt gattgagatt ctcgattttg tcgattttga tgctctacaa ccaaaatgga
      841 tagtaggtta ttcagatatt agtacgttgc tacttgctgt cacattaaaa acagggatag
      901 ccactgcaca tggaactaac cttattgatt taagaggaga gtacttggat gaaacgacag
      961 caatgtggga aagtgtactg aaaacaagag aaggagaatc gattctacag caatcttctg
     1021 aaaagtatca aaaagtgtgg ccccatgagc attcatcatc ttctgttttc catctcactg
     1081 aaccaacaaa ttggcaatca gtcagcggag atgcagtaag gatgaaggga cgattattag
     1141 gtggttgtat tgatacgatc aggcatttaa tcggtactcc ttatggagat gtccaatctt
     1201 ttaaaagaaa gtataatgat ggaaagccga tcttgtggtt tctagaaaac tgtgagctat
     1261 cgtcagttga tttaagaaga tcacttgttc agatgaaact ggcaggctgg tttgatggca
     1321 gctcggggat catgttcggt agaagtgcgg cagaggattt gttagattat aaaatgaagg
     1381 atgtatacga agatctgtca aaggaattaa aactaccgat cttgtatgat attgattgtg
     1441 gtcatgtacc gccacaactg acattgatta atggtgccta tgcagaagta gcggtagaag
     1501 atagaaaagc gactgttttg caaacgttta tttaaaaaat tttttcctgc cttggagtct
     1561 atcaaagacc aataaatcta aatagaaaag aaagtgaaag aagctgacaa aatgctgtca
     1621 gcttcttttt gtataacata ttagaaagtc taattatcta taactgtcac atcgattaac
     1681 tgtttcacgt cattgggatt tcctgtagga atagctacta cagatgcatt atcaatgatt
     1741 tcttgagttg tttctctaat gaacgctgga taagatgttt gatctgcagg catttgccaa
     1801 tgtgaaggaa ctggataact agtgattgtc ccgtcatggt tacttgaata cactaaaact
     1861 ccctggtaac tgtatttccc agtgagcata accacatctt tcggatagac tgcactccct
     1921 gcatcataag gggcgatagg tgttcctgca ggataatgaa taacatgtaa ttcgaaatca
     1981 cccgatgtat ctctaaaagt attacctcta atagctagcc aaacacgtgc gtactcaatt
     2041 tgtagatcag aatatcctgc taacggatct attgactgag aagactgtga ggaggttgtg
     2101 tctattgttg aagaagaaat atttgtctca actgtactac tagatgtaga agaagtattg
     2161 ggctctgaga ctttactgct tgttgaagat gtattcgttt cagtagaagc tgatttctga
     2221 gtacttgatt tttcagctga actggtttgt ctgattgaac ttttgtctgt ggtcgcagat
     2281 ggatcactat tttctttact aggagacgta catcctacaa ggaataaaga gaccaacaat
     2341 aaaataaaac ctaacttttt catataaaat acctcattat ttttttaata ccagtatagc
     2401 ataatcctga atttttgaat ccgtaaaacc ttgtctatga atataaatag agtgtatctt
     2461 ctttgataaa tgtcagaaaa agtgtgacaa ataatcatca gtaaaattac ttgtcacact
     2521 attgataagg agtatttaat tgaagagaat gattaggaga aaggatctcc gtacttttta
     2581 tagtaatcat agaatccaag tttgtataga cgaatcgttt tttctcttat ttcttctgtt
     2641 tcaaaatgga ctgcttgaag aatcaagtct gtgaattcta taggagctgt tccattcgca
     2701 gtaatcaaat tacgatcttt cacagcttgt tgttctataa aatcttcttt attctgataa
     2761 ttttccgatt ctaaccaagg aaattgcgca tttccggtgt gtttgtaacc ttgcaataat
     2821 ccgctctgag ctaaataatc aactgcccca caaatagcag ctactggtac gtctgttttt
     2881 aacaatttat cgattatttg atataaatga tgatttttta tgtcccatga attgccaccg
     2941 atcataataa aaaggtcaac tttttcaggt atttgttcga ttaagtaatc gatttttgtt
     3001 tttaatcctc caatggaagt gacctctgat tggatagaag cagttttgtt ttgccatttc
     3061 tcattttggt ttagtgtgct agtaatataa gcaccttccc aatcggcata ttcctctaat
     3121 agaaacgtaa tcgcagtttt cattttcaag ctctccttta ctatctataa caaggacaac
     3181 agttttaacc tacagacatt cattaaaatt gggtagcgac agcgaaaaca acaattctta
     3241 agttttagac agagtataca gatagtcaac ttaattaaat acgaactaaa taaaaatgac
     3301 aggtaatcat ttttgtttac cagcattata acattttgca tacatggatg acaaaagaac
     3361 caattcccac ttgcttgagt acaatattaa aagaaactgg aaggcaaaaa gtaattatag
     3421 aaacgaaaaa taaatcatat cgggcactca gacatgaaaa caaccctaga gatatataac
     3481 taggtaacca gtttaatgaa agaaaaaatc atccaagaag tcgattcttg gattttttag
     3541 tggatttgtt cgccaaatac gtttagtatg cgtatatgag aggttgtaag caaagcgttg
     3601 tggtctgagc acttaaggag gaatctgtga aaatagcttt tcatattttt gcagattttg
     3661 acttaatgcc gaaggtcagc tttgcgatta ccgtttatcg agaggctgta aaagcaccgt
     3721 gtagctccga tgacaataag caagcatttt tagagctagg tttccttgat tctaaatatg
     3781 cggcttattt caatgagttg gtgcttgaac gcgattttaa gaaggtcgaa aacaggcacg
     3841 acttttcgac gtaaggagaa agaatttcaa tgatagaaac aataaatccc gcagaaaaca
     3901 aggtttctgc gtacattcat atcccatttt gcgagcatat ctgctattac tgtgatttta
     3961 ataaagtttt tttagaaggg cagccagtag atgagtatgt ggaaatgctg ctgaatgaaa
     4021 tgaaaataat gctagaaagt catccgacag cgaatttaga aacactttac gtagggggcg
     4081 ggacaccaac ttctttatct gctaaacaac ttgatcgact gttatcaggt gctagagaga
     4141 tcctgccttt tcaaaatggt aatgagttta ctgtagaagc gaatccaggt gatttgaccc
     4201 gtgaaaaact tcaagtaatg aaaaactatg gcgtcaatcg tttatccatg ggagtgcaga
     4261 catttgataa tcgcttattg aaaaaaattg gacgtaaaca caccgcagaa gatgtgtatc
     4321 aaactatgca atttttagaa gcagaaaatt ttaccaatgt aagcatcgat ttgatttatg
     4381 cattaccggg acaaacacta gaaggatatc tggatacttt ggaaaaagct ttggctttgg
     4441 atctgcctca ctattctttg tactcattga tcctcgaaaa caaaacaatg ttcatgaact
     4501 gggtacgaca aggaagactc gaactgcctg atcaggaaac ggaaaccaga atgtttgaag
     4561 agacgattga agcaatggaa aagcatggac gtcaccaata tgaaataagt aactttgggc
     4621 tctccggaca tgaatctcaa cataacctca tgtactggaa caatgaccac tattttgggt
     4681 ttggagctgg tgctagcggt tatcttggag atattcgtta ccgcaacaaa ggaccaatcc
     4741 aacactattt acgcccatta cgagcaaatg aacgcccggt attggaagaa gaaaaattga
     4801 cacgaaaaaa tcaaatagaa gaagaaatgt tcttaggact aagaaaaaaa acagggatta
     4861 ctaaaaaaca tttctatgat cgctatcaaa aaacaattga atcggtgtac ggtgatgtgc
     4921 taagggaatt agagcaagaa gggttacttg ttaatgaaga agaccgtgtt tatttgacac
     4981 caagagggac ctttttgggg aacaatgtgt ttgagcgctt tttgtttgaa aaatagttta
     5041 aagaatattt gttttttatc tgaatttaaa taaaaaaagt tcaaaaatac aataaaggac
     5101 tattgaaacg ttgatataac tggatttaca tcaattattt agaattagca ctcttgtgtt
     5161 tagagtgcta aaaaatggag tctttctctt gacaggagaa gggcttcttg ctatatttat
     5221 aagtggatta gcacttagaa tatatgagtg ctaatgagag gtgaggaaga tgttaacaaa
     5281 gcgacaaaac gacattttac gtctgatcat ccaacactat acgagtagtg gcgtgcccgt
     5341 tggttccaaa acattgatgt ctgagggagt caaggccagc tcagcaacga ttcgcaatga
     5401 tatgaaagca ttagaagatg aaggattgct gttgaagact cactcttctt ctgggcggat
     5461 tccatctgct ttaggctatc gttattacgt ggatcatttg ttaaaacctg ctcgcgtagc
     5521 agaagatgat ttgctgctga ttcgtcagtc tctaggcaga gaatttcatg aaatcaatga
     5581 gattattaag caatctgctg agatattatc tgagctgaca agctatacga ctttttcatt
     5641 gggacctgaa ataaaagacc gccgactgac tggctttcgt atcgtaccct tgaataatcg
     5701 acagattatt gctatcgtcg taacagatat ggggaatgta gaaagccaag tgttcactct
     5761 tcccgaatat atgggaagtc aggaactaga aaaaatggta cagattgtaa acgaccgtct
     5821 agtcggagat cctctgatga cggtgtacca taaacttcgt acggagattc cgatgattct
     5881 tcataaatat ttccaaacga cagaagggat gtctcagtta tttgacaccg ttcttggtca
     5941 tgtttttgaa gataaggttt atgtcagtgg acaaatgaat ctcttggatt atgagccccg
     6001 tcaagatctt gatcagttca aatcgatgtt ttcttttatg aaagactcag acggacttac
     6061 acaaatgatc actccgatgg atagcaatat tcatatcaag attggtgctg aattaggcaa
     6121 tgatttattg cagaatatga gtatgattca agcaagttac gagatagatg gtcatggaag
     6181 aggtacaata gccttgttag ggcctactag tatgccttat tctcggatgt tcggtctagt
     6241 cgatgtttat cgaagagaac tagcagctaa acttgctgat tactaccgtt cgcttgactt
     6301 gtcaaactcg taatgtgaat aacgaagcta tttgcagctg atcgttctct ctttcttagt
     6361 aagagcaatc aaacagctga ctgaataatg aaaggaagac tgtatcgtga aagaaaatca
     6421 agaagaacta gataaagaaa tgacagatgc tcagccagaa ccggaaatcg atgtcgaagc
     6481 tgcagaagat tcgggtattt cagaagctga agcggaagaa ttcgaaacag cgaaattaaa
     6541 agctgagtta gaagaaatgg aagataaata cttgcgggct cgtgctgaaa tcgcaaatat
     6601 ggcaaatcga ggaaaaaatg agcgtgagca attacaaaaa taccgttcac aagatttagc
     6661 caaaaaatta cttccatcaa ttgacaacct ggaacgtgca ttagccacag aagtgtcaga
     6721 tgatcaaggg gcaggtttga aaaaaggcgt ggaaatggtc ttggaaagtt tgcgtaacgc
     6781 actggaagaa gaaggaattg aaaaaatccc tgctaaagga gaagcctttg atcctaatct
     6841 tcatcaagca gtacaaactg ttccagcgac tgaagataca ccagccgata cgattgtaga
     6901 agtactacaa gaaggctaca aattacatga tcgcgtgtta agaccaacca tggtcattgt
     6961 tgcacaataa ttgtgcggat ataaaactta cactttcaat taaagaagga gacgttaatt
     7021 atgagtaaaa ttatcggtat tgacttaggt acgactaact ctgcagtagc agtattggaa
     7081 ggaaacgaag caaaaatcat cgctaaccca gaaggaaacc gtacaacacc ttctgttgta
     7141 tcattcaaaa atggagaaat ccaagtaggg gaagttgcaa aacgtcaagc tgtgacaaac
     7201 ccaaatacga tttcatcaat caaacgccat atcggtgaag caggttacaa agtagatgta
     7261 gaaggtaaat catatacacc acaagaaatc tcagcaatga tccttcaata tatcaaagga
     7321 ttcgcagaag actatttagg tgaaaaagtc gaaaaagcag ttatcactgt gccagcttac
     7381 tttaatgatg cacaacgtca agcaacaaaa gatgctggta agatcgctgg tctagaagta
     7441 gaacgtatcg tcaatgaacc aactgcagct gctcttgcct atggtttaga taaaacagat
     7501 cgcgacgaaa aaatcttagt attcgacctt ggtggcggta cttttgacgt atctatcctt
     7561 gaactaggag acggtgtctt tgatgtattg tcaacagctg gcgacaacca tctaggtggg
     7621 gacgactttg ataacaaaat catcgactac atggtagcag aattcaaaaa agaaaacagc
     7681 attgacttgt ctcaagacaa aatggcttta caacgcttga aagacgctgc tgaaaaagcg
     7741 aaaaaagatt tatctggcgt atcaagcaca caaatcagct tgccatttat cacagcagga
     7801 gaatctggac cattgcattt ggaaatgaca ttgacacgtg caaaatttga tgaattaaca
     7861 gctgacttag tagaacgtac aaaagtacca gtacgtcaag cgctaaaaga tgcaggttta
     7921 tcacaatcag aaatcgacga agttatttta gtcggtggat ctactcgtat ccctgcagtt
     7981 gtagaagcag tacgtaaaga aacaggaaaa gaaccaaaca aatcagttaa ccctgatgaa
     8041 gtagttgcta tgggtgctgc tatccaaggt ggcgtgatca ctggtgatgt gaaagatgtt
     8101 gttttacttg acgttacacc attgtcatta ggtatcgaaa caatgggtgg agtgttcact
     8161 aaattgattg accgcaatac aacgattcca acaagtaaat cacaagtatt ctcaactgcg
     8221 gcagataatc aaccagcagt agacatccat gtattacaag gtgaacgccc aatggctgct
     8281 gacaacaaaa ctctaggaag attccaattg acagatattc cagcagctcc tcgtggtatt
     8341 ccacaaatcg aagtaacatt cgatattgat aaaaacggga ttgtaaatgt atctgcgaaa
     8401 gatctaggta cacaaaaaga acaaaaaatc acgatcaaat cttcttcagg tctaacagac
     8461 gaagaaatcg aacgtatggt aaaagatgca gaagcaaatg cagaagcaga taaagctcgt
     8521 aaagaggaag ttgacctacg taatgatgta gatgcattgc tattctctgt ggacaaaaca
     8581 ttgaaagaat tagaaggcaa agtggacgaa gaagaagtga aaaaagcaga aacagctcgc
     8641 gatgaattga aagccgctgt tgaagcaaac aacatcgaag aaatgaaaac taaacgcgat
     8701 gcattgaacg aaatcgttca aaacttgact gttaaactat acgaacaagc ggcacaacaa
     8761 caagctcaag aaaacccaga agcagctcaa ggcggagcag atgatgtcgt tgatgctgat
     8821 tttgaagaag tcgatggcga cgataaataa tcagttcact gaatttcgag agaaggccaa
     8881 agttgctact ttggcttttt cttttgaaaa atagtttgaa gtatcggttt ttgtttcaaa
     8941 caaaatatga tatgataaca agcgacgtga aaatgatgag aaccaatagg aggaaagcct
     9001 atggcaacaa aacgtgatta ttatgaagtc ttagggctct caaaaggagc atcagaagat
     9061 gaaataaaga aagcttatcg taagctttcg aaaaaatacc atcctgacat caataaagaa
     9121 cctgatgcgg aagaaaaatt caaagaagta tcagaagcat atgagatttt aagtgacccg
     9181 caaaaacgcg ctgcatatga ccaatacggg catgcaggca cagatccaaa ttacggtgct
     9241 ggtggagctg gtggcggatt tggtggattc ggcggcttct ctggtggcgg cttcggtggt
     9301 tttgaagata tcttcgactc tttctttgga ggaggcggtc ggtcagtcga tccaaatgcg
     9361 cctcgtcaag gtgctgattt gcaatatacg gtcgacttgt cttttgaaga agcgattttt
     9421 ggtgtagaaa aagaaatcaa gtataatcgg gaagaaatct gtcatacttg tggtgggaac
     9481 ggtgcaaaac caggcacaca accaactact tgtcataaat gtcatggtgc aggtacgatt
     9541 aatgtagaac gccaaacacc acttggacga gtgatgagcc gtcaaacttg tgatgtttgt
     9601 catgggacag ggaaagaaat caaagaacct tgtccaacct gtcatggctc aggacatgag
     9661 aagaaagcac attcagttaa agtgaatgta ccagctggtg tagaagaagg acaacaaatg
     9721 cgtttagctg gtcaaggaga agctggagaa aacggcggac catttggtga tttgtatgtg
     9781 gtcttccgtg tagaagaaag tgatatcttt gatcgtgaag gttctgaaat ttactatgaa
     9841 ttgcctttaa gttttgtaca agcagctttg ggggatgaag tccaagtacc aactgtccat
     9901 ggagatgtga aattgaagat ccctgctgga acacagacag gtacgaactt ccgtttacgt
     9961 ggaaaaggcg ctccgaagtt acgtggtggt gcaacaggcg accaacatgt caaagtaaaa
    10021 ctgatcacac cgaaaaatct aaatgaacaa caaagagaag cattacgtgc attcgctgaa
    10081 gctggcggca tcaaagttga agaacagcaa gaagacggtt tcttcgataa gtttaaagat
    10141 gcttttggcg ggaaaaagcg gaagtagttt ttctagtaaa aacgctaagt cattaataat
    10201 taaaaaccag acgaacaatc atatgttcgc ctggtttttt tgtagttgtc atgtttgtac
    10261 caacaaaaaa gccagcttta gaatatgata atcgtcatag tttctagggg tataaccagt
    10321 gagagaatgg aacttgttca atcgatattg caaggtgttt ttatgaataa ataattcttc
    10381 tgagcaattt ttgatgcttc cgttattttt actgaagcat gccaaagtct tcttgaacaa
    10441 atgtacttct tcttctgtca ggcttttcaa aatacgtttt acatataatg ttcgttgatc
    10501 tgacgggatt gctggtaaaa cgatcccgag gtctaaatct tcaaaaaagc ggatctcgtt
    10561 cacagattga tggaaagcta tccattcgca aacttgtttg gcatgaagga aagattgcgg
    10621 tgtctcttgc tctgtataac aaagcgaccc aattccaaag tttagctgta actgatacat
    10681 tttattaaac tttgtctgga ttttatcaag caatgatagc aatgaggctc tggaaatccg
    10741 attgactagg ataatgattt gttgtctatg tacagtgagc aaagcctgtc cgtaataaga
    10801 gaagaccctt tcgagcaact ccaataaagg attgatccgt tcttgacaca attgctgatt
    10861 gtcagaaagt tgtcccacaa ctacataata tggagaagaa aaatcaagct caattaagtg
    10921 agggtggtat cgactatgta cttgatatca tcaatgaacc gatggtactt tcttatgcga
    10981 tggaacaggt agagcgattg cttgtttgtg caggaaaaca agcgatgcgg ctgatacgat
    11041 taggtgagaa aataagaaga taaaaataca cggtcccttc atggcgaccg tgcatttttg
    11101 gtttttatta aatatttaat actttattca agaaatcttt tgtccgttca ttttgcggat
    11161 tttcaaagat ttgttgaggc gttccatctt ctaaaaagtt tccgtcgtca atgaacatga
    11221 cgcgattggc cacttcttta gcgaacccca tttcgtgcgt aacgataacc atcgtcatac
    11281 cttgttctgc taactttttc atgacgttca agacatcacc gaccatttca ggatcgagtg
    11341 cagaagtagg ctcatcaaaa agcatgatgt cagggttcat cgctagagca cgagcaatag
    11401 ccacacgctg tttttgtcca ccagaaagca tttcaggata catatctttt ttatcttcta
    11461 gaccaactgt ctgcagtaat ttgattgctt ttttctctgc ttcttctcta gattctttct
    11521 tcaggtccat cggtgctaaa atgatgtttt ccaaaataga aagatgaggg aataggttaa
    11581 agtgttggaa taccattccg atatgttgtc tcaccaagtt gatatctgtg cttttatcca
    11641 tcaagtgggc gccgtcaata ataatttcac cgtcagaagg ttcttctaaa cgattcaagc
    11701 aacgcaagaa tgtacttttt cctgaacctg aagggccaat gatacagacg acatctcctt
    11761 cgttgatgga tacattgatg tcgtttaata ctgtgttatc gccgtatttt ttgactaaat
    11821 gctcaactaa gattttttct gccatcttac ttcaccttct tttctaaaac ttttgctaat
    11881 tttgtcaatg ctgtgatcaa aatcaagtac atgatcgcaa tgatcaagta aacatacgta
    11941 ctttgtgttg tacgtgcaac aatgatctta ccagtttgca gcaattcgac tacaccgatt
    12001 gcggaaatga tcgttgtgtc ttttaatgag ataacgaact gattgacgaa tgatgggatc
    12061 atgatgcgta tcgcttgcgg caaaatgatt ttttgcattg tccgattata agaaagacct
    12121 aaacttctgg aagcttccat ttgtccgaca ggaacggcgt tgatcccgcc tcgaacaatc
    12181 tctgcgatat aagcacttgc gttaagagtc aaggtgataa caccagcaag gaagtcagga
    12241 atcgtaaatc caacgatacc aggcaaccca aagaaaataa agaacgcaag aaccatcatt
    12301 ggaattcctc ggatgatatc gacataaata ctagcaattg tgcgaagagc gcgtattggt
    12361 gctacactga ataatccaaa aatcacaccg attactatag ccaaagcaaa ggagatcaaa
    12421 gttaggagaa ttgttctcca aagtcctttc aacaatgctt gatagttgtt ttggataagt
    12481 ccaagaaatg ttgattcatc agcagtggaa gactcactgg aagtttctga accatccgct
    12541 acatattctt taataatctt atcgtattcg cctgttcttt ttaattcttt caatccttca
    12601 ttgaacatat taagaagctc aggattttgt ccttttttaa cggcaaatgc atattttccg
    12661 ccagattctt ttttgatcgg tgtttccaat tcttgtcctt gtttgatcgc ataaccgatt
    12721 accggataat catccatcat tgcatcgatt gcgccaactt ttaacccgtc gtacaattga
    12781 tcagcagcat caaaatattt gatagaatag ccatattcag attcatggtc gcttaaaaat
    12841 tcagcacttt cggtaccaac ttttgctccg actgtttttc ctttcaaatc ttcataagac
    12901 ttgatcgaag tatctccttt tttgacagct aattggatcc cgctatcaaa gtaagaatca
    12961 gaaaaatcat agcttttctt tcgttcatct gttacagtca ttccggcaat cattccgtct
    13021 gcttgtcctg attccaaagc ttgcaaagca gcagagaaac cacggtagtc cattcggatg
    13081 ttaaatcctt gcagtttggc gatgttttcc atcaatttta catcgatgcc ttcgtactcg
    13141 ccctttgcgt tttgaaattc aaatggagca aaagcagagt cactggcaat tacgtatacg
    13201 tcttttttgg gctctatttt ttgcattgtc tcgcttgtgc ttgactctgc cgttgtagtg
    13261 ttgtcagctt gtgcaacatg accaaacgca aatgtcacaa gtcctgtcat taagaaaaca
    13321 aatgagtgaa ataaatgctt tcttttcata atcaacctcc taaatttcca atattttatt
    13381 attataggcg aaaagtgctg aatcagacaa gactgtaagc tcgaaaaaat gaaaaaaatg
    13441 cgagtttcta acattttatg ttataaaaca taacatgtat aataagataa aaaagtggcg
    13501 aaagtatgtt cggtgtggta taatttttta gtaaggaatg cctttgcttt ccttttcttt
    13561 ttattggtcg taaaatgaaa aggaatgaat gaaaaggagg acgaagaatg attgagagag
    13621 atacctcccg acactttgag cttgtctcga aataccaacc ggctggtgat cagcctgaag
    13681 cgattaacca attagtggat ggcgtagtcg gcgggaaaaa agcacaaatc ttattaggtg
    13741 cgactggaac agggaaaaca tatacgattt ccaatcttat tgagaaagtg aataaaccaa
    13801 cactgattat tgcccataac aaaacattgg ctggtcagct gtacggggaa ttcaaggaat
    13861 ttttccctaa caatgctgtt gaatattttg tttcttatta tgattattat cagcctgaag
    13921 cttatgtgcc ttcaagcgat acgtatatcg aaaaagattc aagtgtaaat gatgagatcg
    13981 acaaattacg acattcggct actagttcat tattagaaag aaatgatgtg atcgtcattg
    14041 catccgtttc atgtattttt ggattaggct cgccttttga gtaccaaaaa caagtagttt
    14101 cgattcgaca gggagcagaa ctagatcgca atcagttgat tcgtgactta gtcagtatcc
    14161 aattcgaaag aaatgatata gattttcagc gcggtcgttt tcgagtgcgc ggtgatgtcg
    14221 tggagatttt tccggcttca agagatgaac gtgccttacg tgtggagttt tttggagatg
    14281 aggtcgaacg tatccgagaa gtcaatgcac tgacaggtga agttttagga gaaacggaac
    14341 acgtagcaat cttcccggcg actcactttg tgacgaatga tgaacatatg gagcatgccg
    14401 tagcgaatat caaagcggaa ttagaacagc gtttaactgt cttgcgaaat gaaaacaaac
    14461 ttctggaagc tcaacgttta gaacagcgaa ccaattacga tattgaaatg atgttagaaa
    14521 tgggctacac gtcgggaatt gaaaattatt ctcggcatat ggacggacgt aaagaaggtg
    14581 aaccgcctta tacgctattg gatttctttc ctgaagattt tttgatcgtg gcggacgaat
    14641 cgcacgtaac catgcctcaa atcagaggaa tgtataatgg tgaccgagcg agaaaacaga
    14701 tgctagtaga ttacggtttc cgtttgccat ctgctttaga caatcggcca cttcggttag
    14761 aagagtttga aaaacatgtc aatcagatca tttatgtctc tgcgacgcca ggaccatatg
    14821 aacatgaaca aacagatacc gttatccaac aaatcatccg tccgacaggg ctgcttgacc
    14881 cagtgatcga agttcgtcca atcatgggac aaatcgatga tctagtaggc gaaataaatg
    14941 aacgagtcga aaaagaccag cgggtgtttg taacgacatt gacgaaaaaa atggcagaag
    15001 acctcacaga ttatttcaaa gaattaggga tcaaagtcaa atatttgcat agtgatatca
    15061 aaacgctgga acgaacagaa atcattcgag atcttcgatt aggtgaattt gatgtgttag
    15121 ttggaatcaa tttgcttcgt gaaggattag acgtacctga agtttccctt gttgctatct
    15181 tggatgcgga taaagaaggc tttttacgta gtgaacgttc cttagtccaa acgattggac
    15241 gagctgctcg gaatgaagaa ggtaaagtaa tcatgtacgc agacaaagta acagattcta
    15301 tgcgtctagc gatggatgaa acgtcaagac gtcgtacgat tcagcagaag tacaatgaag
    15361 aacatgggat cgttccaaaa acgatcatta aagaaatccg agatttgatt tctattacga
    15421 aagaatcgga agatgataca aaagaagcgg tacaagtctc gtacgaagaa atgacgaagg
    15481 aagaaaaaga cacgttattg atgaaattag aaaaagaaat gaaagatgcg gcaaaagctt
    15541 tggattttga aacagccgcg aatgtgcgag atatgatttt agaattaaaa gcttcaaagt
    15601 aggaggacaa catggctaac gataaaatta cgattcatgg tgcacgtgcc cataacttga
    15661 aaaatataga tgtaacgatt ccacgagata aattcgtggt cgttacaggt ttgtccggtt
    15721 ctggaaaaag ttccctagca tttgatacgc tgtacgctga agggcagcgg agatacgtag
    15781 agagcctttc tgcttatgct cgtcagtttt taggacaaat ggataagccg gacgtagata
    15841 gcatcgacgg attgagtccg gcgatttcta ttgatcagaa aacaacaagt aaaaatcctc
    15901 ggtcgacagt aggtactgtg acggaaatca atgactattt acgcttgttg tttgcacgtg
    15961 taggacatcc gatttgtccc aacgatcata tcgagattac cagtcaatca gtagatcaaa
    16021 tggcagatca agtaatggct ttgcctgagc gcacaaagat ccaagtgttg gcaccgatcg
    16081 tggtaaagaa aaaaggacaa cataaaaaaa tattcgaacg aatccaaaaa gagggatacg
    16141 tccgcgttcg agtcgatggt gagatctatg atttaagtga agtgcctgag ctggagaaaa
    16201 acaaaaaaca tgatatcgcg atcgtcatcg accggatcat tgtgaaagaa gggattcgtt
    16261 ctcgcctatt tgattcttta gaggcggccc tgcgattagc agacggctat gctctggttg
    16321 acgtgatcgg tgaagaagaa atgttgttta gcgaacacta tgcctgtcct tattgcggat
    16381 ttactgttgg cgaactagaa ccaagactgt tctcattcaa tgcgcctttt ggagcttgtc
    16441 cagactgtga tggtttaggt gtcaaattag aagtcgatca agatttagtg attccagata
    16501 gaacgaagac acttagtcaa ggtgctatcg ttccatggaa tccaatcagc tctcagtatt
    16561 atcctcaaat gttagcgcag gcttgtgaaa gtttcggtat cgatttagat acaccattcg
    16621 aagaattacc ggaagagcat caggagatca tcttgaatgg gacagatgat cttttccatt
    16681 tccattatga aaatgatttt ggtggcgttc gcgatgtaga aacgactttc gaaggggtat
    16741 tgaagaatat cgagcgtcgt tatcatgata cgaacagtga tttcacgcgt gagcaaatgc
    16801 gtctttatat gacagaatta acttgtcaga catgtaaagg ttatcgattg aatccccaag
    16861 ctctatctgt tcaaatcaat ggaacgaata tcgggcaaac aaatgagttg tctattcaaa
    16921 aagcagtaga tttcttctca aacttaacgt tatctgaaca agagaaagta attgccaaac
    16981 cgattttaaa agaagtaaat gacagacttt cattcttgga aaatgtggga ctagactatc
    17041 taactttgag tcgcgcttcc ggaacattat ctgggggaga agcgcagcga atcagattgg
    17101 ctacacaaat tggatcgaat ctttctggag ttttgtatat tttagatgaa ccatcgatcg
    17161 gattgcatca acgcgacaac gatcgactga ttggctcatt aaagaaaatg cgtgatcttg
    17221 gcaacaccct cgttgtagtc gaacatgatg aagatacgat gtgggcagca gattatttga
    17281 ttgacgtcgg acctggggcc ggagaacaag gcggacaaat cgtggctgca ggcactcctg
    17341 aagaagtagc taatgatgaa aattctttga cagggaaata tctttcaggg aaaaaatcaa
    17401 tccctgtacc aaaagaacgc cgtaaaggta atgggaaagc aatcaagatc actggtgctt
    17461 cagaaaacaa tctgaaaaat attagtgttg aatttccatt aggagaattt gtagcagtca
    17521 caggtgtgtc tggttctgga aagagtacat tggtcaatag tatcttaaag aaatcgttag
    17581 cgcaaaaatt aaataagaat tctgctaagc caggtaaatt caagacaatt tccggctacg
    17641 aaagtatcga aaagatcatc gatatcgatc aaagcccaat cggccggacg ccgagaagta
    17701 atccagcgac ttatacaagt gtatttgatg atatccgtgg gttatttgct caaacgaacg
    17761 aggcaaaaat gcggggttat aagaaagggc gttttagttt caacgtaaaa ggcggtcgtt
    17821 gtgaagcttg tcgcggggat ggaattatta agatcgaaat gcactttttg cctgatgtct
    17881 atgttccttg tgaagtatgt catggtaaac gatataattc tgaaacatta gaagtgcatt
    17941 acaaaggaaa aagcattgct gatattttgg aaatgacagt agaggatgct gtagaattct
    18001 tcaagcacat tccaaagatt catcgcaaac tgcaaacgat tgttgatgtt ggcttaggtt
    18061 atgtgacgat ggggcaacca gcaacgacat tgtccggtgg tgaggcacaa cggatgaaac
    18121 ttgccagtga attgcacaaa atctctaatg gaaagaattt ctatatacta gatgaaccaa
    18181 cgacaggact tcatagcgat gacatcgccc gcttgttgca tgtattacaa agattagtag
    18241 atgctggtaa cacagtttta gtgattgaac acaatctaga tgtaatcaaa acagcagatt
    18301 atatcattga tttaggacca gaaggtggag aaggtggagg aacgatcctt gcgactggaa
    18361 caccagaaga aatcattaac gtaaaagaaa gttatacagg tcactatttg aaaaaaataa
    18421 tggtataaag aggatagata gctgagatag ttaaaaaaac ataaaaaaat gaatttttgc
    18481 ttgttttatt aatatcagtg ttctagaatc attacatggt agttctttct acactgattg
    18541 gtatagataa aaacttccta taagttcaat ccgttcaaaa aagaattgag caatcggagg
    18601 actttccttt gattgctttt ttctttatgg tatgatattc cttgtgtagt tggaaaagaa
    18661 aaacaagaga ttatgagtaa gtgagagata atgatgaaca gtattgaaaa tgaagaattt
    18721 atccctatat tattaggaag cgacatgaac gtttatggta tggcacgttc ttttaatgaa
    18781 gcatacggca aaatctgcca agcatatgct tcggatcagt tagcccctac ccgctacagt
    18841 aaaattgtca atgtagaagt tattccagga tttgataaag accctgtatt tatcgagacg
    18901 atgcttcgtt tggcaaaaga aagatattct gataagagta aaaaatatct tttgattgct
    18961 tgtggtgatg gatatgcaga attgatctcc caacataaac aagagctgtc agaatatttt
    19021 atctgtccat atattgatta ttctttattt gaaaggctga tcaataaagt aagcttttat
    19081 gaagtttgtg aagaatatga tcttccgtat cctaaaacat tgatcgtcag agaagaaatg
    19141 ttagtaaatg gacatttgga acaggaattg ccttttgaat tcccagttgc tttgaaacct
    19201 gcaaacagtg tggagtatct ttctgtccaa tttgaaggaa gaaaaaaagc ttttatttta
    19261 gagactagag aagagttcga tttaatttta ggccggatat acgaagccgg ttataaaagt
    19321 gaaatgattg tacaagattt tatcccgggt gatgatagta atatgcgtgt gctaaatgcg
    19381 tatgtagatg aagatcatca agttcggatg atgtgccttg gtcatccact attagaagat
    19441 cctacaccag catcgattgg taattacgtt gtgatcatgc cagattataa cgaaaaaatc
    19501 tatcaaacca ttaaggcatt tttggaaaaa atcgaatata caggctttgc caatttcgat
    19561 atgaagtatg atcctagaga tggagaatat aaattatttg aaatcaattt gagacaaggt
    19621 cgtagcagtt tctttgtgac attgaacggc ttgaatctgg ctcgttttgt cacagaagat
    19681 cgagtgttca acaaaccctt tgttgaaacg acctatggaa cgaatcaatc agataaagca
    19741 agattatgga tgggtgtgcc caaaaaaatc tttttagaat atgctcggga aaatgaagac
    19801 aaaaaactag ctgagcaaat gatcaaagaa aatcgttacg gtactactgt attttatgaa
    19861 aaagaccgat caatcaagcg ctggttgttg atgaaatata tgttccataa ctatattcca
    19921 agattcaaaa agtattttca tgtgaaagaa ggctagttca tggagaattt tttcagtatt
    19981 ttaggcggaa tgggcacgat ggcgacagaa agttttgttc gcttgatcaa ccaccgaacg
    20041 aaagctacaa aagatcaaga atatttgaat tatgtcttat tcaatcatgc aacagttcct
    20101 gatcgtacag cttatatttt agatcgatca gaagaaaatc caatgccgtt tctgctggat
    20161 gatattgaga aacaaaattt attgcgacca aacttcattg tgttgacttg taacaccgca
    20221 cactactttt ttgaagagtt acaagcggca acggatattc ctatcttgca tatgccaaga
    20281 gaagcagcaa atgaacttgt ccgtcagcat acgacaggga gagtagcgat tctaggaaca
    20341 gaaggaagta tgaaagctgg aatctatgaa cgcgaagtca aaaatcttgg tttcgaaaca
    20401 gtgattcctg atactgcgct tcaagaaaaa atcaattacc tgatctatca tgagatcaag
    20461 gaatcggatt atttgaatca agagctctat tacgaaatct tagaggaagc ggtagagcgc
    20521 ctaaactgtg aaaaagtcat cctaggttgc acagaattat ctttgatgca tgaatttgct
    20581 gaagataatc attacccagt gatcgatgct caatctattt tagcggatcg aacgatcgaa
    20641 cgtgcattag ctgaaagaag cgaggcattg gatacagcat cggaaaagta aaaaaattgg
    20701 atttggggca aatatcctaa aagatatttg gtccaaatct ttttgtaaaa gaaaaagaaa
    20761 actggtaatg atacgcccgg gatgatataa ttttattatg cacaatgtga gaggggttcg
    20821 taaatatgac agataatttg caattagtca tcataacagg tatgagtggt gcagggaaaa
    20881 cagtagctat ccaaagtttt gaagatatgg gctatttttg tatcgataat atgccgcctc
    20941 gattgattcc aaaattctgg gaattgatca aagaatcagg taaagtcaca aaaatggctt
    21001 taggagtgga tttgcgttct cgttccttct ttgaagaaat ccaaaacatg ctgatcgaaa
    21061 tcgaaaatac gaacttcatc gacacttcta tcttattttt agatgcttca gacgaagaac
    21121 tagtggctcg ctacaaagaa acaagacgag cacatccaat ggcgatggac ggtttgatca
    21181 ctgaagggat acgtaaagaa cgggcgatac tagacgactt gaaagcccaa gcttccgtga
    21241 tcattgatac gacaacattg acaccacgac aattacgtga aaaaatcaat gagtcattca
    21301 aatcaaaggg agaccaagga tttcggatcg aatttgtatc atttggtttt aaatacgggt
    21361 tgccgatcga ttcggatatt gtgatggatg tgcgtttttt gcccaaccct cattatatcc
    21421 cggaactaag accattgacc ggaaaagatc agccagtcta tgattatgtg atgtcctttg
    21481 atgaaacgca atcattctac cataaatttt tggatcttct ggaaactgta ttgcctggat
    21541 acattaaaga aggaaaaagc agcttgacag ttgcaatcgg ctgtacaggc ggtcaacacc
    21601 gttctgtggc attgactgaa cgtttggcaa atgctttagg gaaaaactat aaagtcaatg
    21661 tgacacatcg ggacaaggat aaaaggaaag agacggtgaa ccggtcgtga aaatgaaaac
    21721 ctaccggata agaaaaccaa agatcgtagt ggtcggcggt ggcactggtc tgccagttat
    21781 cctgaaaagt ttacgaaatc aaggagcaga tatcacagca gtagtgactg ttgcagatga
    21841 tggtggaagc agcggtgcaa tcagagaatc gatcgctatg gctcctcctg gtgacttgag
    21901 aaatgtctta gtggcgttat cggatatgcc acaattctat gaagatattt tccaataccg
    21961 tttcaaaaag gaagatcagt ttttagccaa tcatacgatc ggtaatctga tcattgccgc
    22021 agtttccgag atgcggggaa gcacttatga ggcgattcaa ttattagcaa aaatgatgca
    22081 tgtagatgga catgtttatc cttcttcaga aactcctttg accttgcatg ctgtttttaa
    22141 agacggaacg actgcagtag gagaatctaa aatcgcgatc aaccgtaaaa ccattgaccg
    22201 tgtgtatgtc tgcaacacaa atgataaaag tgaagccaaa gcggctcgaa aagtcgtttc
    22261 agcaattatg gacgccgaca tggtcgtttt gggacccgga agtttattca ccagtatcct
    22321 tccaaattta gtgataccag aaatcggcga agcaatgctc aagacaactg ctgaaaccgt
    22381 ctatatctgt aatatcatga cccaaaaagg ggaaacggaa catttcacag atgccgatca
    22441 tatacgtgtt ttacacaagc atttgaatca gccttttatc gatacagtat tggtcaatac
    22501 ggaaaaagtg cctgaagatt atatggaccc agagatctat gatgagtatc tagtacaagt
    22561 cgcacacgac ttcaacggat tgagaaatga aggatgtcga gtgatttcca ccgacttttt
    22621 agaattgaaa gatggtggtg tcttccacga tggagacaaa gtagtagaag aattgttccg
    22681 tttagttttc ggttcaaaat attaagcaat ttattgttgg agaaaggagg agaataggat
    22741 gtcatttgct gcagatgtga agaaagaact aactggtttg gaagttcatc gagaacatgc
    22801 gaaagcggaa ttagctgctt tgctgcgaat gaacggctct ttaggtattg tgaaccagca
    22861 gtttattttg aacgtccaaa cagaaaatgc tgcaattgcc agacgtatct attctctttt
    22921 gaaagatcat tatcaggtac gaagtgagct actcgttcgt aaaaaaatga aattaaaaaa
    22981 gaacaatgtc tatatcgtcc ggttgaagca aggcacaaaa gatgtgttga tggatcttga
    23041 tatcatggat ggtatgatgt tcaatggaca cgtttcggac gagattatgg gaaattcgca
    23101 aaaaatgcgt tcttacttac gtggtgcttt tatggcctca ggttcagtca ataatccgga
    23161 aacgagtcgt tatcatttgg aaatattttc tatttatgaa cagcataacc aagacatttg
    23221 cgatatgtta aattattacg gtctacatgc tcgaacgctt gaacggcgca atggctatat
    23281 ttcgtattta aaaggagcag aaaaaattgc tgatttctta acattgattg gtgcaaccaa
    23341 ctcaatgctc cgatttgaag atgttcgaat cgtacgggat atgagaaatt cggtcaatcg
    23401 tctagtcaat tgtgagacag caaatctgaa caagacgatt gatgcggctt ctaaacaaat
    23461 cgaaaacatc gaattgatcg aagcaaaggt cggtctacat gcccttcctg aaaaacttca
    23521 ggaaatcgca gaacttcggc ttcaacatcc agaggtcagc ttgaaagaat taggggaaat
    23581 gattccctca ggtgcaatat caaaatcggg aattaatcac cgaattcgca aaataaatga
    23641 atttgcggaa aaactgcgtg aagaagcagt ttaatccaaa gaaaaaactg atacatgaag
    23701 caggagatgc gaatgtatca gtttttttta aaagctatac ttgcttttct tccgtcaaac
    23761 tataattcca actttactgg agtaacataa tgttgaaagg cttctattgt tttttaatac
    23821 aatcgtataa aaagcgggga gacaactgaa tgaagatttt agttttatac ataa
//
LOCUS       DACDMM010000024        23830 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDMM010000024 DACDMM010000000
VERSION     DACDMM010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.024"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(54..788)
                     /locus_tag="IWD16_000760"
     CDS             complement(54..788)
                     /locus_tag="IWD16_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9087843.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="IWD16_000761"
     CDS             1115..1525
                     /locus_tag="IWD16_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP9087844.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="IWD16_000762"
     CDS             1518..2219
                     /locus_tag="IWD16_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP9087845.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="IWD16_000763"
     CDS             2219..3805
                     /locus_tag="IWD16_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9087846.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="IWD16_000764"
     CDS             3824..4045
                     /locus_tag="IWD16_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP9087847.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="IWD16_000765"
     CDS             4042..4803
                     /locus_tag="IWD16_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP9087848.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="IWD16_000766"
     CDS             5183..5692
                     /locus_tag="IWD16_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9087849.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="IWD16_000767"
     CDS             complement(5767..6306)
                     /locus_tag="IWD16_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP9087850.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="IWD16_000768"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="IWD16_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP9087851.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7373..8170
                     /locus_tag="IWD16_000769"
     CDS             7373..8170
                     /locus_tag="IWD16_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP9087852.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8198..8833
                     /locus_tag="IWD16_000770"
     CDS             8198..8833
                     /locus_tag="IWD16_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP9087853.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="IWD16_000771"
     CDS             8853..9626
                     /locus_tag="IWD16_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9087854.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="IWD16_000772"
     CDS             10034..10831
                     /locus_tag="IWD16_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP9087855.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="IWD16_000773"
     CDS             10809..11222
                     /locus_tag="IWD16_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087856.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="IWD16_000774"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="IWD16_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9087857.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="IWD16_000775"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="IWD16_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP9087858.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="IWD16_000776"
     CDS             15999..16559
                     /locus_tag="IWD16_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP9087859.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="IWD16_000777"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="IWD16_000777"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP9087860.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="IWD16_000778"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="IWD16_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP9087861.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="IWD16_000779"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="IWD16_000779"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP9087862.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="IWD16_000780"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="IWD16_000780"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP9087863.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="IWD16_000781"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="IWD16_000781"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP9087864.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="IWD16_000782"
     CDS             21906..22484
                     /locus_tag="IWD16_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9087865.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="IWD16_000783"
     CDS             22536..23018
                     /locus_tag="IWD16_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP9087866.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="IWD16_000784"
     CDS             23135..23509
                     /locus_tag="IWD16_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087867.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4285 c         5114 g         6649 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatagaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt
//
LOCUS       DACDMM010000025        23213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDMM010000025 DACDMM010000000
VERSION     DACDMM010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.025"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            338..694
                     /locus_tag="IWD16_000785"
     CDS             338..694
                     /locus_tag="IWD16_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9087868.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            704..1603
                     /locus_tag="IWD16_000786"
     CDS             704..1603
                     /locus_tag="IWD16_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP9087869.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(2000..3079)
                     /locus_tag="IWD16_000787"
     CDS             complement(2000..3079)
                     /locus_tag="IWD16_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9087870.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            3311..4123
                     /locus_tag="IWD16_000788"
     CDS             3311..4123
                     /locus_tag="IWD16_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP9087871.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            4120..4920
                     /locus_tag="IWD16_000789"
     CDS             4120..4920
                     /locus_tag="IWD16_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP9087872.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            5081..6349
                     /gene="rseP"
                     /locus_tag="IWD16_000790"
     CDS             5081..6349
                     /gene="rseP"
                     /locus_tag="IWD16_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP9087873.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            6417..8126
                     /locus_tag="IWD16_000791"
     CDS             6417..8126
                     /locus_tag="IWD16_000791"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP9087874.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            8338..12696
                     /locus_tag="IWD16_000792"
     CDS             8338..12696
                     /locus_tag="IWD16_000792"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP9087875.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            12841..13314
                     /gene="rimP"
                     /locus_tag="IWD16_000793"
     CDS             12841..13314
                     /gene="rimP"
                     /locus_tag="IWD16_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP9087876.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            13337..14512
                     /gene="nusA"
                     /locus_tag="IWD16_000794"
     CDS             13337..14512
                     /gene="nusA"
                     /locus_tag="IWD16_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP9087877.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            14534..14827
                     /locus_tag="IWD16_000795"
     CDS             14534..14827
                     /locus_tag="IWD16_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP9087878.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            14824..15135
                     /locus_tag="IWD16_000796"
     CDS             14824..15135
                     /locus_tag="IWD16_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP9087879.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            15148..17454
                     /gene="infB"
                     /locus_tag="IWD16_000797"
     CDS             15148..17454
                     /gene="infB"
                     /locus_tag="IWD16_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP9087880.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            17481..17828
                     /gene="rbfA"
                     /locus_tag="IWD16_000798"
     CDS             17481..17828
                     /gene="rbfA"
                     /locus_tag="IWD16_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP9087881.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(17985..18158)
                     /locus_tag="IWD16_000799"
     CDS             complement(17985..18158)
                     /locus_tag="IWD16_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9087882.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            18606..20968
                     /locus_tag="IWD16_000800"
                     /pseudo
     CDS             18606..20968
                     /locus_tag="IWD16_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            21336..22259
                     /gene="truB"
                     /locus_tag="IWD16_000801"
     CDS             21336..22259
                     /gene="truB"
                     /locus_tag="IWD16_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP9087883.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            22263..23204
                     /gene="ribF"
                     /locus_tag="IWD16_000802"
     CDS             22263..23204
                     /gene="ribF"
                     /locus_tag="IWD16_000802"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP9087884.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
BASE COUNT         7749 a         4194 c         5117 g         6153 t
ORIGIN      
        1 agtcttttat ttgataagtt tactctaccg aagagttgtt aataatccgt gaacaaaaag
       61 taattctttt tccaatagtt gttaaaggtt ttttattttt tatagtattt tttgttattt
      121 aagaaaggaa taaggttaaa caaataacag gttgcttttt gggtcttttt ccgttcaaga
      181 aactgaacga agaaaaggga aagttgtctc ttttgtgata agagaaatca taataaacga
      241 atgacttact ttccaataaa ttgagaacag tcagaaccag acaaattagt tgcatctgat
      301 gaataaagtg attattctat gacatgaggt gatacagatg aaattagaag tgacaaaaga
      361 agcacaagaa gtattgaaga ataaattaga agcagacgat caacttttat tagatatcga
      421 aaacggtgac ggtccatttg cagaaagcca agtcagttgc caattggata cggcatttcg
      481 attgatcatt gtcaaaaaag atacacaaac agatctaagt ctttattctg tagtggctga
      541 tacaccagtt ggaccaatca aaatcaaaga cagtgcaata ctttacatgg atgatccatc
      601 cacacttgct ttagaaccaa cttataatag tttacaatta aaaggaccaa gcggcttgag
      661 aaaaggcaac ttgcaagttg ttcgaaaaga ctagagggat aaaatgggaa aattggcgat
      721 catcagcgac ctacatgtgg acatcaataa attcggcgaa tcagagctga tgcagctttg
      781 gcaagttttg caaaagcaca agatcactcg gcttcatcta gccggagata cggctaataa
      841 agtagaccgt tgcctagctg ttgtcgaatt tttcgtcaat aaaggattat ctacgacatt
      901 tcatttcggc aatcacgagt tggcagatgt gcccaatgaa cagatgatgg accattttcc
      961 agacccacat tttttaaatg aacggtatat tgctttaaat gagcagaccg ttcttttagg
     1021 gatgaatggc tggtacgact atcagttttc cgaattgaaa gattacgagg caatcgtacg
     1081 aatgaagcgt ttgtattggt atgatcggtt catccagcgt gggaaaacag atctcgaggt
     1141 caatgcagaa atgcttgcag ctgcaaaaaa tttgctagac cagctggaag caaaaaaact
     1201 tgaggtgatt ttatccactc atttcgttcc taaacgggca tttatcatgt atcaatctgc
     1261 tccttatgag cgttggaaca aattaaatgc atttttagga tcagaatcat tcggagaatt
     1321 acttgaccgt tattcaaata tcaaacaagt agtttttggc catacgcatc gtcgctttga
     1381 agataaatcc atcggtcaca cgatttacag ctgcagacct tttggttatt attatgaatg
     1441 gcagctgacc cgagattttg tcttgaaaga acagttgcta gacagctatg atcctatgaa
     1501 attacgtgtc ctcttgcgtg cccatcaccc agcttttcag gcctatcaaa cacaacattt
     1561 gcaagaggaa tttgaacaag cgatgacaat cgttccgtat tgagacagca ttagtaatgt
     1621 gtaaaagaaa ataccttgta aaacataagg ataagtaaga gcagtagatc catttatcgt
     1681 ggatatgctg ctttttttgc ggaaaataat cagcgaatgt ttatttgatc ggatgatttg
     1741 taactcgcag agtactagat gcaagcgtgt attctttttt tgaacgataa tcacaaaaaa
     1801 aaatataatc gtaaatttca agtttaagtt agccacccat ggtgacccgg ttgctcgtta
     1861 ccaccaaccg tctaattttt ctaatcgagt gggcccttga caatgagctg aggcatggta
     1921 gtctcccgac tggctggccc cggccagctt tccagacaca agccatgccg agggaggctc
     1981 gttgtcaagg gcccacgtgt caagccgtca accgttcctg cctaaatgcc tggacgaacg
     2041 tgtcatgcgg cgttgcatag cccaaaatct ttcgcgggta atcgttcatc caacgctgaa
     2101 tccgcaagat ttggttttcg ctcacgttgc cgattgggct gcccttgggg atgaaacggc
     2161 gaatcagctt gtgttggttt tcgctcgtcc cgcgctccca ggaagcatag gggtggctga
     2221 agtagatgtt tgccacatcc tgcaaggcat cgtgcagtcc cgcgaactcc gagccgttgt
     2281 ccgaggtaac cgtcttgaac agctggccga agtacgcacc agcccgttct ctcagctgtc
     2341 ggaccgcttg gttgaccgat tcggcatcct tcccggccag cttcagaatg acctcgaagc
     2401 gtgtctgccg ttccaccaac gtcagcagca cggcatccga cttggtcttg tttcctacga
     2461 ccgtgtcgac ttcccaatgg ccgaaggttt tccgggtacc aaccgcctcc gggcgctctt
     2521 caatggattt ccctagcacc cgtttattcg ggcggttgcc atgacgggac gatttcggtt
     2581 tgcgggacag tttttctagc aggtccatat tcttcgtttt catgatgcca cggtcgatcc
     2641 actgatagag ggtggtggtc ccagggataa tggccgggtc gaacaattcg tgttttctgg
     2701 caaagcccac caccacgtcc ggggaccatt tttccaacag catcatgtcg tccgcccatt
     2761 caatgaaggc gtccgtgtcg gcccataagg gccgtctgcc gcagttcaag cggttcttct
     2821 cgtaggccgc ctgcccggca tcggcgtcgt agacgtcatg ataatagtcg tagaccttcc
     2881 cgttctgatt ctggcggtta agctgagtaa cggtcccgcg tttgatttcg ttgtttatgg
     2941 tctgcggcac gcagcccagg acatccgcaa cctgacggtt ggaatagttt tcctttttca
     3001 ggatggcgat ttggcaacgt tctgagtagg ttaggtgttt tcccttgcgt gaaactgtgt
     3061 tatcatggtt ttgcgtcatg tgaattcatc cttgtctatt gagagttgtg gtaacttcaa
     3121 tataacatgg aattcacgtg gcgttttttt atttccaaat ggctaagtta attataaaat
     3181 ccgccaaatt aaaaaaagaa aaaattattc agtttttaaa taaaaaaaca atgatttcat
     3241 aggaaaaaca gttagatatt gctataatac ttaagatagt gggaatcttt taagtatagg
     3301 aggaaaaaga atgttacgtt tttttccgca aaaaaacaaa tatattcaag aaaaaagcga
     3361 atttcaattt aataccgatg gaccaattcc tcagcacatt gccatcatca tggacggcaa
     3421 tggtcgttgg gcgcaaaacc gtcgtttgcc acgagttgct ggccataaag aaggcatgga
     3481 aacagtaaag aaagtcacaa aaaaagcatc aaggcttgga gtgaaagtgt tgacgcttta
     3541 tgccttttcg actgaaaact ggaagcgacc aaaagatgaa gtaagttttc tcatgcaact
     3601 acctgtcgac ttctttgata cgttcgtacc agaattgatt aaagaaaatg tcaaagttca
     3661 tgtcatgggt tatgaaaacg tattgccaga acatacgcaa gatgctgtaa gacgtgcaat
     3721 tgagcaaaca aaaaacaata ctggaatggt attgaatttt gctttgaatt atggaagcag
     3781 agcagagatc gtgactgctg tccaagaaat tgccgaagaa gtagcaaaag gagaaataca
     3841 cgcagaagaa atcgatgatg aattgattgc taagcattta atgacaggtt tcttgcctaa
     3901 agagttacaa gatcctgagc tgatgatccg tacaagtgga gaagagcgaa ttagtaattt
     3961 tctgttatgg cagatcgcat atagtgagct gtattttaca aaagcgcttt ggcctgattt
     4021 tgatggtgcg catcttgaag aagcaatcgc ctcttatcaa aatcgagacc gtcgattcgg
     4081 cggtgtaaaa aaaacagaag atgagaaagg ggatcagtca tgaaacaacg tgtggtcaca
     4141 gcagccgtgg cgctagtctt gtttattccg attatatgga tcggaggcat agcggttgag
     4201 cttacagcag caatcttagc agtaatagga gtttacgagc tgtttcggat gaaaggattg
     4261 acactcttaa gttttgaagg agttttatcc gccgttggtg cagtgttact tgttttgcca
     4321 aaagaaagat ggtttttctt tcttccagag aatgtaagta catttatgtt attttatctt
     4381 atagttatga ttcttttagg aacatcagtt atttctaaaa atacgtatac gattgatgaa
     4441 gcaggttttc ctgtgcttgt cagcttgtat gtgggaatag gtttccagaa ttttgtcaat
     4501 gcacgaacag aaagcttggc tgttttgata tttggtctat ttattgtttg ggcaactgat
     4561 atcggagcat atatggttgg tcgacgttat ggtcagcata agttaagtcc tgatatttcg
     4621 cccaacaaaa cgattgaagg tgcgctcggt ggtattggta gtgctgttgt agtcgcattt
     4681 ctttatttat tgctataccc agcaaaagaa ctatttaatc atggtatgtt tgtcatgctt
     4741 ctgatgacga ttattttttc agcagtcgga caatttggtg atttggtcga atctgcaatc
     4801 aaacgtcatt atgatgtgaa agactcggga aatatcttac caggccatgg tggaatctta
     4861 gaccgtttcg acagtttgct gtttgtattt cctatcatgc atttatttgg cattatttag
     4921 taagtatacc tgcgtttctt tgtgaatttc ataaaagacg caggttttta tttttcatta
     4981 tattgatact attggtgtct ttggtatact atggtaaaat tatagtgttt tatcgttgtt
     5041 ttataaaaaa cacaaaaaac aagtaaagga gaaaaaagct atgaaaacga ttctgacatt
     5101 tatcatcgtt tttggtatat tagtgattgt tcatgagttt ggtcatttct tctttgcgaa
     5161 acgatcagga atcctcgttc gcgaatttgc tatcggtatg ggtccgaaaa tttatgggca
     5221 tcaagcaaaa gacgggacaa catatacgct tcgcctatta cctattggcg gttatgttcg
     5281 aatggcaggc aatggggatg atgaaacaga aatggcacca ggaatgcctc tttctctttt
     5341 actaaattct gacggcatcg tagagaagat aaatttaagt aagaaaatcc aattgacaaa
     5401 tgctatcccg atggaattga gccgttatga tttagaagat gagctgacta tcactggtta
     5461 tgtgaatgga gacgaaacag aagtagtaac ctaccctgtt gatcacgatg cgacgattat
     5521 tgaaaacgac ggaacagaaa tcagaattgc gccaaaagac gtacagttcc aatcggctaa
     5581 attgtggcag cgtatgctga ccaactttgc tggacccatg aataacttta ttttggcaat
     5641 cctgttattc atcattctgg catttatgca aggtggtgtt caagtgacga acactaatcg
     5701 cgtaggagag atcatgccta atggagctgc ggcagaagca ggcttaaaag aaaatgatga
     5761 ggtagtcagt gtggacggaa aagaaatcca ttcatggaat gatctgacaa cagtcattac
     5821 gaaaaatccg gataaaacat tagactttaa gatcgaacga gaaggtcagg tgcaatctgt
     5881 cgacgtgaca ccgaaatctg tagagtcaaa tggcgaaaaa gttggccaac ttggtataaa
     5941 agccccaatg aatacaggtt ttatggataa gatcattgga gggacgcgtc aagctttcag
     6001 cggttcattg gaaattttca aagccttagg atcactattt acaggtttca gtttagataa
     6061 attaggcgga cctgtcatga tgtaccaatt gtcttcagaa gcagcaaatc aaggtgtaac
     6121 aactgttatt agtttgatgg cgcttctttc aatgaatctc ggaatcgtca atctgcttcc
     6181 gattcctgcc ttagatggcg ggaaattagt cttgaatatt tttgaaggga ttcgcgggaa
     6241 accattaagt caagaaaaag aaggaatatt gacattagct ggctttggat ttttaatgtt
     6301 gttgatggtg ctagttacat ggaacgatat tcaacgattc tttttctaga aaaagttgac
     6361 aaatagggta taataacaaa cgataactat tttgaggaaa taaaggagta tatagaatga
     6421 gacaatcaaa aatgttgatt ccaacattac gcgaagttcc aaatgatgca gaagtattaa
     6481 gccatcaaat cttgctaaga gctggatata tccgccaagt atcagcgggt atttattcat
     6541 atctgccatt agcaaatcgt gtattggaaa aactgaaacg cattatgcgg gaagaatttg
     6601 aaaaaatcgg agcggtagaa atgctgatgc ctgccatctt gccagcagaa ctttgggaag
     6661 aatccgggcg ttacgaaaca tatggaccaa acttgtatcg tttcaaagac cgcaatgatc
     6721 gaaaaatgat ccttggacca actcacgaag aaacttttac tgatttgatg cgtaatgaaa
     6781 tcaattcata taagaaatta cctttaaatc tttatcaaat ccaacctaaa tatcgtgacg
     6841 aaaaacgacc tcgcttcggt ttgcttcgcg gaagagagtt tatcatgaaa gatgcgtatt
     6901 ctttccatgc aacggaagaa agtttggacg agacatataa agattacgaa aaagcctaca
     6961 ccgaagtatt caagcgttgt ggcttagaat ttcgctcgat tatcggagac ggtggcgcaa
     7021 tgggcgggaa agactcaaaa gaattcatgg cgatctcaga aatcggggag gatacgatct
     7081 gttattcgac tgaaagtgat tatgctgcaa acttggagat ggctacaagc tactatgtat
     7141 caaaaaaatc gcatgaaaca cagcttgaat tggagaaagt agctacacct aacgtaaaat
     7201 caattgacga agtagcagat ttcttttctg ttgaacctca aaaaatcatc aagtctgtat
     7261 tgttcattgc ggatgaacaa cctgtccttg ttttggtacg cggagaccat gaagtcaatg
     7321 acgtaaaact gaaaaacttt ttatctgctg actttttaga agaagcaaca gaagaagaag
     7381 cacaaaaata tctaggtgct gagtttggtt ctgtcggacc agtcggtgtt tcagatgacg
     7441 taaaagtata tgcagaccgt tatgtccaag atttagcgaa tgccattact ggagcgaacg
     7501 aaacaggatt ccactataaa aatgtgaatc cagaacgcga tttcactgta ctttcttatg
     7561 aagatcttcg ttttgtacaa gaaggagatc cttcaccaga caacaatggt gttcttgcct
     7621 ttactcgagg aatcgaaatc ggacatatct tcaaaatcgg cactcgttac agtgaatcta
     7681 tgggagcaac tgtactagac gaaaatggac gagaaaaatt tgtgattatg ggctgttatg
     7741 gtatcggtgt aagtcgttta ttatcagcaa tcgtagaaca acattcagat gaacaaggaa
     7801 tcaactggcc aaaaggaatt gcgccatttg atttgcatat cgtccaaatg aatctaaaag
     7861 acgaatacca aacaaacctg acgaatgaac tagaagaagc gatgacaaaa gctggctatg
     7921 aagtactggt tgatgacaga aatgaacgcg caggagtgaa attcgcggat tctgatttga
     7981 ttggctgtcc aatcagaatc actgtcggta aaaaagcagt ggatggaatc gtagaagtga
     8041 aaatcaagaa aactggcgaa atggttgaag tacgtaaaga agaattggtg aatactttac
     8101 caatcctctt gaaccaaggc gaataataag cgttaaaaca cagaaaataa aaatagctga
     8161 atcagctgtt tttgacaatc tgatgattcg cttttaaaat tggaactgcg atttgaatcg
     8221 cagttccaat tttttaatgt aaaactcaga aaaaacgagt atactaaaag gtactgaaaa
     8281 agaagaggat aagatgcgat caattgcatt gtttctcatc agaaagggcg tatatctttg
     8341 tcagaaaaac gagatttatt tgaaaaactg ttggaacaaa tccaactaga ggaagcagaa
     8401 aaaaatcatc cactgcttac tgctggtgaa atggatagtg tagtggtcca tcgtaaatcg
     8461 cgtttatggg aatttacgct gcatttttcc aaaattttgc ctattatgct atatcggtca
     8521 ttgactcagc atctgactat cgcttttaaa gatattgcac aagtcaaact gaatatctta
     8581 gcagatgacc agacattcga tgaacagctc ttgcaagatt actgggccca agctttggaa
     8641 aatcagcaat gcgatacacc actagcacag caagtattga aaacgcaggt acctgtgata
     8701 aaagatcgta aagtcatctt gccaatcgac agtacaggag ctatttctta tttgaagcaa
     8761 caatacttgc cattagtaga agagttgttc gtcagttacg gattcccaaa atttcgtatc
     8821 gaaccagaag tggatgagca gcaagcggaa cgagtattga aactttttga agaacgcaaa
     8881 caagaacagg cagaagcctt tatgaagcaa gcagctgaaa gtctagttgt ccacgaacaa
     8941 aagaaaaagg aaagaaaaga gcaatcgcca gctcttgaag gacccatcca catccagcta
     9001 ggacgaaata ttccagccga tgaaccaacg acaccaatga tcagtatcgt tgaagaagaa
     9061 cgaagagtca cacttgaggg gtacgtattc gacaaagaag tcagagaact acgttcaaaa
     9121 cgcaagatct tgactttgaa aatcacagat tatacttcat catttatcgt caaaaaattc
     9181 tcgaacggcg aaaaagatga acaagtattt gatgcgatca gtgtcggtag ctggttaaag
     9241 gtacgaggaa gtattcaaga agatacgttt gtccgtgatt tggtcatgaa tgcgcaagat
     9301 atcatagaag tcaaacatac acctcgcaaa gattatgctc ctgaagggga aaaacgagta
     9361 gagcttcatg ttcatagcaa tatgagtacg atggatgcaa caaacagcat ctctgatctt
     9421 gtggcacaag cagggaaatg gggacaccga gctatcgcca ttacagacca cggtggtgcg
     9481 caagcctttc cggaagcaca tagtgctgga aaaaaagcag gtgtgaaaat cttatacggc
     9541 gtggaagcga atgtcgtaga tgatggtgtc ccaattgctt ataatgatgc acatgaagca
     9601 ttgagtgaag ccacatatgt cgtatttgac gtggaaacga caggattgtc tgcagtatat
     9661 gacacgatca tcgagctagc cgcagtcaaa atgtataaag gaaatgtgat cgaaagcttt
     9721 gatgaattta tcgatccagg acatcctttg tcacgaacaa cggtagatct gactggtatc
     9781 acggatggaa tggttcgagg atcaaagtcg gaagaagaag tattgcggat gttccttgaa
     9841 ttttcaaaag atacaatttt ggtagcccac aacgcggctt ttgatatggg gttcttgaat
     9901 actagctacg caaggtatgg tattcctgaa gcggccaatc cagtcattga tacgttggaa
     9961 ttagctagat atctgtatcc tcaattcaaa cgtttcggat taggtgtgtt atctaaaaaa
    10021 tttggtgtca gcttggaaca gcatcaccga gcaatctatg atgctgaagc aactggtcac
    10081 ttagcatgga tctttgtgaa agaggcaatg gacaatcata acatgcttta tcatgatcag
    10141 ctaaatgagc atatcggaga aggagattct tacaaaaggg ctcgaccttt ccatgtaacg
    10201 attttagcaa aaaatcaggc aggactgaaa gatttgttca aactgatttc aatgtcgaat
    10261 gtcgaatact tcgaacgcgt cccacgaata ccacgctccc aattgaaaaa gatgcgtgaa
    10321 aatctattga tcggctcagc ttgtgataag ggagaaatat tcgaagccat gatgcaaaaa
    10381 ggggtagaag aggcaagaaa ccgagcaaaa ttctacgatt atattgaagt tatgccaaaa
    10441 gcggtctatg cgcctttgat cgagcaagaa ttggtaaaaa atgaacatga tttggaagaa
    10501 attatccaaa atttggtaga aatcggaaaa agtttggata aaatcgttgt tgcaacaggt
    10561 aatgtacatt atctgaacga agaagatgcc atttaccgaa aaattttgat caattctatg
    10621 ggcggagcga atccgttgaa tcgtcatagt ttgccggatg tccatttccg aacaaccgat
    10681 gagatgctaa ctgcatttca ctttttaggg gaagaaacag cgaaagagat cgtagtagaa
    10741 aatacgaata aaatcgcaga tatctgcgaa gaagtgattc cagtcaaaga tgaactttat
    10801 acaccgaaga ttccaggttc agaagatgaa atatcagaat tgagttacac aaaagcaaaa
    10861 caaatgtacg gagatccgtt acctgagatc attcaaaaac gattaaaaaa agagttgaat
    10921 tctattaatg gtaatggctt ctcggttatt tatctgatcg cccaaaaact agtgcataaa
    10981 agtaatgaag atggctatct agtaggttca cgtggctctg ttggctctag ctttgttgcg
    11041 acgatgacag gaatcactga agttaatccg ttagcccctc attattattg tccagagtgc
    11101 caatattccg agttttttga agatggtacg tatggttctg gatttgacat gccagaaaaa
    11161 caatgtccaa aatgtggtgc acgcttaaac aaagacggtc atgatattcc atttgagaca
    11221 tttttaggat tccatggaga caaagtgccc gatattgatt tgaacttctc aggtgattat
    11281 caagcagaag cccataacta tacgaaagta ttgtttggcg aagactatgt ttaccgtgcc
    11341 ggaacgattg gtacagtcgc cgacaaaaca gcatatggct atgtaaaagg atacgaaagg
    11401 gacaataatc tgcagtttcg aagtgcagaa gtcgacagat tagcaaaagg cgctactgga
    11461 gtcaaacgga caaccggcca gcatccaggg gggatcatcg ttattcccga ttatatggat
    11521 gtctatgatt ttacgccgat ccaatatcca gcagacgatc aaaattcgga atggaaaacg
    11581 actcattttg attttcactc gattcacgat aatgtattga aattagatat tctagggcat
    11641 gatgacccaa ctgttattcg gatgcttcaa gatttatcag ggatcgatcc acaaacgatc
    11701 cctacggatg acccggaagt gatgcggata tttgccggtc ctgaagtact tggagtaagt
    11761 caggaacaaa tttattccaa aacagggaca ttaggtattc cagaattcgg cactcgtttt
    11821 gttcgaggga tgctagaaga aacgcatcct acaacttttg cagaactact gcaaatttcc
    11881 ggactatctc atggtacaga cgtatggtta ggaaatgcgg aagaattgat tcgacgagga
    11941 gatgcgacac tggcggaagt aatcggctgt cgtgatgata ttatggttta tttgatccat
    12001 gctggattag atagcgggat ggcatttaaa atcatggaaa ctgttcgtaa aggacaatgg
    12061 aacaagatcc ctgatgaatt gcgagaaacc tatctatcgg cgatgaaaga aaataatgtg
    12121 cccgactggt atatcgattc ttgttcaaaa atcaaatata tgtttccgaa agcccatgct
    12181 gcagcttacg tattgatggc tcttcgtgtc gcttatttca aggtttattt ccctattttg
    12241 tattactgtg catacttttc tgtacgtgca gacgactttg atctagtctc catgtgcaaa
    12301 ggaaaagatg ctgtgaaaca agcaatgaaa gaaatcacgg acaaaggatt agatgcttcc
    12361 gtaaaagaaa agaatcaact gacagtgctg gagttagcca atgaaatgct ggaacgagga
    12421 ttcaaatttg gaatgattga tttgtataaa tcggatgccg ttaactttgt tattgaaggt
    12481 gatacgttga tcgcaccatt tagagctgtc ccaagtttgg gaaccaacgt ggcaaaacaa
    12541 atcgttgaag caagaaaaga cggtccattt ttatccaaag aagatcttgc cacccgcggt
    12601 aaagtttcaa aaacattgat cgaatatatg aacgacaatg gcgtattgaa agatctacca
    12661 gatgaaaatc aattatcgtt attcgatatg ctataagcat ctgttttcct gtctgcttgt
    12721 aattttaagc agagtatggt atagttctta taggtaatga tactaactgt gagtgagcgg
    12781 atttttcgct cactcttttt aatggaatta cgcacttaat tttttaagga ggcgaaactt
    12841 ttgagtagcg tcgttgaaac agtcacagaa atggtgacac cgattttaga tgaacaaaat
    12901 tttgaacttg tcgaagtcga atttgtaaaa gaaggaaaaa gctggttctt acgagtattt
    12961 attgacaaag aaggcggtat cgatatcgaa gaatgcgcct ttgtcagtga gaaactgagt
    13021 gaaaaacttg atgcagcaga tccagatccg attcctcaag cttatttcct ggaggtatcc
    13081 tccccgggag cagagcggcc attgaaaaaa gaaagcgact atgaaaaggc acttggtgat
    13141 tatgtccatg tctccttata ccaaccagta gatggcgaaa aacaatatga aggatttctt
    13201 caatcatttg atgctgaaca gcttacattg aaaatacgca taaaaacacg agaaaaagaa
    13261 ttgacttttg accgaaaaaa tattgcaaaa gctcgcttag ctatccaatt ttaatcacgg
    13321 agaggaatca gaaaaaatga gtaaagaaat gttaaatgcg ttagacgctt tagaagctga
    13381 aaaagggatc tcaaaagaaa tcgtgatcga tgctttagaa gcagcattgg tttctgcata
    13441 caaacgtcac tatggccaag cacaaaacgt cgaagtggaa tttgatcaga aaaaaggaaa
    13501 gatccatgtc tatgcggtaa aagaagtaac ggaagaagtg atggattcac agttggaagt
    13561 ctcattgaaa gatgcacttt taatcaatcc agcatatgag atcggtgata cgatccgttt
    13621 tgaagtaaca ccaaaagatt ttgggcggat cgctgcgcaa acggctaaac aagttatttt
    13681 acagcgtgta cgtgaagcag aaagaacgat tatttataac gaattcagtg cttatgaaaa
    13741 agacatcatg caaggaatcg tcgaacgcca ggataaacgt tatatttatg tcaatcttgg
    13801 taaaattgag gcagttcttt ctaaacaaga tcaaatgcca aatgaatttt atcaaccaca
    13861 tgatcgaatc aaagtttatg tttcccgagt agaaaatacg tctaaaggtc cgcaagtatt
    13921 tgttagccgc agccatccag acctattgcg ccgcttattt gagcaagaag taccagaagt
    13981 ctatgatgga atcgttgaaa ttgtcagcat cgcccgtgaa gctggcgatc gttcaaaagt
    14041 ggcagttcgc tcaaatgatc caaatatcga tgcagtcggt acatgtgtcg ggccaaaggg
    14101 acaacgagtt caagcaatcg tcaatgaatt aaaaggcgaa aacatggata tcgtcgaatg
    14161 gaatgaagat ccagctgtat tcattgcaaa tgcattgaat cctgcacaag tcatggatgt
    14221 gatcttcgat gagacaaatc caaaggcctg cacagttgtc gttccagact accaattatc
    14281 gctagcaatc ggtaaacgcg gacaaaacgc acgtttagct gctaaattga ctaaccataa
    14341 aatcgatatc aaatcagaat ctgacatgac ggaattttat gaaaatcaag aacaacaaaa
    14401 agaaacagaa gaactgcatg atgaagcaat cgttcagtca gatttaacag acgatgaata
    14461 tgaaacaatt gcattcaaca atgaaacggt cgaagaaaaa ccagaagctt aactttagta
    14521 aggaggcaaa gagatgaaac aaaggaaaat tcctttacgc aaatccgtgg tttcaggtga
    14581 aatgaaacct aaaaaagaga tggtccgaat cacccgttca aaagaagggg aagtatcgat
    14641 cgatccaact ggaaaaatgc ctggacgagg agcgtatgtc tcacttgagc cggaagaagt
    14701 acaacaagct tgggataaaa aaattctcga tcgagtactc gaaacaacat taacagatga
    14761 gttttatcaa gaacttcttg actatgtcac tcaccaaaaa gcacgtaaag agttatttgg
    14821 aaaatgaata cagttaatcg gcaaaaagcc atgaatctca tcggtctagc gatgcgcgcg
    14881 ggaaaactaa tcacgggtga agaactgacc attgctgaaa tccgcaaaca aaaagcaaaa
    14941 ttggtgtttg tggcaaccga tgcaagcgag aatacaagaa aaaagataaa agataagagt
    15001 tcatattatg aagttccttg ctttgagcta ttttctgaag cagaaatcac gcagatgatc
    15061 ggaaaacctc gcaaggtatt cggaataatg gatgccggat ttgcgaagaa gaccaaggag
    15121 ctaattgaag gttaggaagg tgattgcatg ggcaacaaaa gaatctatga attagcaaaa
    15181 gaactcaaaa aatctagtaa agacgtcgtg gaaaaagcac aacaacttgg catcgatgtg
    15241 aaaaaccata tgggtgcgat tactagcaat gatgagaaga aattgcaaca agcgttcaaa
    15301 aacccaagca cgaaacaaca agctgcacaa caagcaagca gaaaaccagc caatcaacag
    15361 ccgaacgatc aacgtaaaaa agaaacaacg aacaatcaac aaaaaaataa aaataaccgc
    15421 agttatcaag atcgcggtca agggagcggt caagtgaatc aaggaaaaaa accaacaaca
    15481 aaccaaaata atacgggaaa taccagcagc caaaatcgtc aaggaacgag ccaaggcggc
    15541 aatcaaaacc gtactaacaa taacaacaaa ccaaacaata acaatcgcgg caaatttaat
    15601 aataataacc gtaatcgtta taataaaaaa ggtaaaaaag gcaaacagca acaatcaact
    15661 aaaccagctg taccaccacg caaattccgt gaattgccag atgtattaga gtatactgaa
    15721 ggtatgaacg tagcagatat tgcgaaaaaa attcatcgtg agccagcaga aatcattaaa
    15781 aaattattta tgcttggtgt catggtcaat caaaaccaag cactggataa agatacgatt
    15841 gaactgttag cgacggacta tggcatggaa ccacaagaaa aagtccaagt tgacattgct
    15901 gatatcgata aattcttcga ggcagaagaa gtcgatccag ataaattagt ctcacgtccg
    15961 ccagtcgtaa cgattatggg acacgtagac catgggaaaa caactttatt ggatacatta
    16021 cgccattcac gtgtaacaag cggcgaagcc ggcggaatca cacagcatat cggtgcctac
    16081 cagatcgata ttgatggtaa accaatcact ttcttggata caccaggaca tgcggccttt
    16141 acaagtatgc gtgcacgtgg agcaagtatc acagatatca cgatcttagt tgttgctgca
    16201 gatgatggcg tcatgccaca aacagttgaa gcaatcaacc atgccaaagc tgcaggcgtt
    16261 ccaatcatcg tcgctgtaaa taaaattgat aaaccaggtg caaaccctca acatgttatg
    16321 caagaattaa gtgagtatga gttaattcct gaagcatggg gtggagaaac gatctttgtt
    16381 gagatctcag cgaaattcgg ccaaaacatc gaagaattgc tggaaatgat cctgttagtc
    16441 gcagaagtag aagatttaaa agcagatcca acacaacgcg caatcggaac agtgatcgaa
    16501 gcacgtcttg ataaaggtaa aggtcctgtg tcaaccttac ttgtccaaga aggaacactt
    16561 catgttggtg acccaatcgt tgtcggtaat acttacggac gtgtccgtgt aatgacaaac
    16621 gatcttggcc gtcgcgataa agaagcagga cctgcaacgc cagtagaaat cactggcttg
    16681 aatgatgtac ctcaagcagg tgaccgtttc gtagtcttcg aagacgaaaa gacagcacgt
    16741 gcagcgggtg aagaacgcgc gaaacgtgca ttattagaaa accgtgctgc cacaagccgt
    16801 gtgactttgg ataacttatt tgaaagcctg aaagaaggcg aattgaaaga agttaacgta
    16861 atcatcaaag cagatgtaca aggttcagca gaagcactgg ctgcatcatt gaagaagatt
    16921 gatgtggaag gtgtccgtgt aaaaatcgtc cattcagctg tcggagcaat caatgaaagt
    16981 gatgtaacct tagcagcagc cagcaacgct attatcatcg gtttcaacgt tcgtcctaca
    17041 ccgcaggcaa aagtgcaagc tgatactgaa gaagtcgata ttcgtcttca ccgtatcatc
    17101 tataaagcca tcgaagaaat cgaaactgcg atgaaaggga tgctagaccc tgaatttgaa
    17161 gaaaaaatca ccggtcaaat gatcgttcgt gaaaccttca aagtttctaa agttggtacg
    17221 attgcaggtg cctatgtaac agaaggatat attcgtagag atagcggtgt ccgtgtaatc
    17281 cgtgacggaa tcgtaatcta tgaaggccaa cttgcaagtc tgaaacgatt caaagatgac
    17341 gtgaaagaag tcaaaatggg ttatgaatgt ggtgcaatga ttgaaaaatt caatgacatc
    17401 aaagtagatg acgtaattga aggatttatc atggaagaag tcaaaaatga ctaaaaactg
    17461 ataaaaggag gccaatcatt atggctaact accgtgaccg ccgagtcggt caagaaatct
    17521 taaaagaagt caatgatatt ttgcgcaaaa aggtccgtga ccctcgtgtg gagaacgtaa
    17581 cgatcacaga tgtgaaagtt acgggggact tacagcaagc aacgatctat tacagcattt
    17641 tatctgactt agcatcagat aagaaaacag cacaagatgg attgaataaa gcatcaggat
    17701 tgattcgtcg agagttaggg cacaatctaa gtatttataa gacacctgaa ttgttctttg
    17761 aacttgatga atcagttgtc tatggcaacc atatcgatga attgcttcgc gacttgaaca
    17821 aagactaatc aaaaactccc gcaaatcctg tgaaatcagg ggttgcggga tttttttgtt
    17881 ttcattaaga cgtattacct aagagttcca tgactttagt cttgcggcgt gtcagctaac
    17941 caaaaaagag aaaaagaaaa gtaaaaaatt cgactttctt ttttctagta gtatataaat
    18001 ttttgcatat agataaagat caagataccg ataacggaag ctagattgta atctatttta
    18061 gtgacagtca ggtatagcca agtaactaga agatcagtgg aacctgcacc ataaaagttt
    18121 gagtttgtcg gtccgttgac agttaaaaga aaaatcacgt tgaagttatt gatatttccg
    18181 attacgccgc ttcgatttgg tcacgatcca aattttggat aatccctgta gaaattagca
    18241 tagtagctgg gataccaatt cacatattga caaagataac agagactttt acataaaacg
    18301 gatcaatcag gaaaggaatt gctcgatcca gccccagttt tccagcaatg tgttaactgg
    18361 cccagcgtca ttcagcaaat tatgcatgat gaggagcgaa atgaactgcg gaacagccat
    18421 tgtgataacg acaagttttt tggatcaatg taaaaaacgt cagcgaaagg aaaaatttcg
    18481 tggtttttta tacctgttca tttggctaga tataaaatcc aaacaaatga acagaagtgc
    18541 ttattaggtt tcaattggga gtatctgcgc tagaataaga gtaataatga aaaggagtgt
    18601 tatgtatgga ttattctagt aaagaatact ttgataaaat gacggcttgg tggcgtgctg
    18661 ctaattatct ttctgttggg caactttatc ttaaagataa ccccttactg agacgtacac
    18721 tgaaacccga agatgtcaaa aaacatccaa tcggtcattg gggaacgatt cctggccaaa
    18781 attttattta tgtccatttg aaccgagtca tcaataaata tgatttgaac atgttttata
    18841 ttgaagggcc aggacacggc ggacaagtca tggtatcgaa tgcttattta gatggaagtt
    18901 acacagagat ctatccagaa gtaacggaag atgagacagg aatgcaaaaa ttattcaaac
    18961 gattttcatt tcctggaggc attgcttctc atgcagcacc agaaacacca ggttctatcc
    19021 atgaaggcgg agagcttgga tactctcttt cacatgcagt cggagctgta ttagacaatc
    19081 ctgaagtaat ctctgctgtc gtgattggtg atggtgaagc agaaaccggt cctttagctg
    19141 gaagctggtt ctcaaatgtc tttatcaatc cagtgataga tggagctgtt ttaccgatcc
    19201 ttcatttaaa tggtgcaaaa atagccaacc caacgatctt ggcacgtaaa tcagatggag
    19261 aattagcaaa ttactttaac ggacttggct gggaaccttt ctttattgaa ggaaatgatt
    19321 cagaaaaatt aaatcctgtt atggctgaaa aaatggatca agcaatcgag aaaattaaat
    19381 ccatccaaaa agaagctcgc ctgaagacag caacagatgt cgtgatgcca aaatggccag
    19441 tattgatcgt acgcacacca aaaggatgga ccggtccaaa agaatgggac ggggaaccaa
    19501 ttgaaggaac cttccgagca caccaagtac cgattcctgt agatcaagaa catatggatc
    19561 atgcagatgc tttactccgt tggctgaaat cctatgaacc agaaaaatta tttgatgcac
    19621 aaggacgtat tttagaagaa atcagagaaa tcgcgcctac tggtgaccaa agaatggcaa
    19681 aaaatcctat caccaatggt ggaatcgatc caagaccgct tattatgcca gattggaaaa
    19741 aatatacttt gcaatttgaa aaaccaggct ccgttaaagc agaagatatg accgaacttg
    19801 gtaaattcgt tcgcgagatc attgagaaaa atccagaaaa tttccgtata tttggaccag
    19861 atgaaacaaa atcaaaccga ttgaatcaag ttttcaaaac gacaaatcgc caatggatgg
    19921 aaaaaatcga accagaaaat gatgaatggc tttcgccatc tggtcgcgtc atcgacagtc
    19981 aattatcgga acaccaagat gaagggttct tagaagggta cgttctgact ggacggcatg
    20041 gttttttgca agttacgaat cattcttgcg agtggttgac tctatgttga ctcagcactt
    20101 caaatggatg cggaaatcac atgacctttc ttggcgaaat gattatcctt cgctaaatct
    20161 cattgcaagc tcaacagtct tccagcaaga tcacaatgga tatagccacc aagatccagg
    20221 aatattgaca catttggcag aaaagaaggc ggagttcatc cgtgaatatc tgcctgcgga
    20281 tgcaaataca ttgctcgcag tcatggataa agcattccgc tcgtcagaaa aaatcaattt
    20341 gatcatctct tccaaacatc cgcgtgctca attttactca gcagaagaag cagccgtctt
    20401 agtgaatgaa ggcttgaaaa tcattgattg ggcttcgaca gcaaaagagg aggaacctga
    20461 attggtcatt gctgctgcag gaacggaatc taacttagaa gcattagcag ctgtaacttt
    20521 gctgctggaa gaattcccaa aactaaaaat tcggttcatc aatgtcgtag atttattgaa
    20581 attacgtcat ccaagtcaag atccgcgtgg gttgagcgat gaagaatttg atcaatattt
    20641 tacaaaagac aaaccgatct tatttgcttt ccacggttat gaaacattgg tccgcacgat
    20701 tttctttgat cgtcataacc atcatttaat gattcatggc tacaaagaaa atggagatat
    20761 cacgacacca tttgacatgc gtgtagtaaa tgaacttgat cgttatcatt tggcaaaaga
    20821 cgcagcttta aaaatcaaag gaagtcaagc agaagatttt gcgaaaaaaa tggatcagaa
    20881 attacaagaa catcaaaact atattcgtga aaatggtatt gatctgccag aagtattgga
    20941 ttggaaatgg aaaaatcttg accaataaaa tgataaaatc gatcccgcaa aatagaattt
    21001 tttacattga ttaagaaact gaaaaaataa ataaaaaaaa cggaaaaaag actggaagaa
    21061 tggccagtct tttttttcgt ttttgggaag tgaaaaccaa actatttacc tgtaagccac
    21121 ccataaatcc aatcaaatgg tgcaaagaaa aaatcttcca ttctattccc tcctttaaaa
    21181 aagcataaca tgatttctat ttacgtgagc atgaattttg ttacgttttt attgccgaaa
    21241 aataaaaacc gttcattatg atgtcaaatt cgtcagtaat tgatgcaatg tgttataata
    21301 attcagttaa atcaaaaatg aaatggagaa aagacatgga cgggttatta cctttatgga
    21361 aagaacgcgg aatgacaagt catgattgcg tattcaaatt aagaaaaatc ttacatacga
    21421 aaaaaatcgg acatgggggt acgcttgacc cagacgtaga cggtgtgttg cccatttgta
    21481 ttggaaaagc cacaaaagtt atcgaatatt tgacagattc tgggaaaatc tatgtaggcg
    21541 agataactct aggtttcagt acaacgacgg aagatcgatc cggagaacgg gtagaaacaa
    21601 aaccagtaga tatgtcttta tctgaaaaaa aaatagatga agtgatggct tcatttattg
    21661 gtgagatcac ccagatacca ccaatgtact cagcggtaaa agtcaacggt aagcggttat
    21721 atgaatatgc ccgcaataat gaaacagtgg agcgtcctgt tagaaaggct cagatttacc
    21781 gttttatccg gacaagtgaa ttgacaagag acgaagtgac aggaaccatt tcttggcgct
    21841 tcgaagtaga atgcgggaaa ggaacctatg tccgcacatt agctgtagat acaggcaaga
    21901 aacttggtta ccctgcgcac atgtctgatc tgacccgaac agcaagtgcc ggtatgtccc
    21961 agaaggatgc tctgacatta gaagaagtag ctcaagctgt gaaaaatggg acaatcgatg
    22021 attgtttgct gccaatagaa aaaggagtgg aacggttcaa acgagtagat attactgatg
    22081 aagtatggca gaaagtaaaa aacggtatga gacttgatta tactgtattt gcgctcccag
    22141 aaatgccgtc agaagaaatt gctttgtttt ataaaggaaa agttgtcagt atttatcagc
    22201 caaacccgaa agaaaaaaac aaattaaaac ctagtaaagt attgagaaat gaggaataac
    22261 gaatgaatat catcaagatc agacatcctt accaatcaga acaaattcca gaagacgatg
    22321 tcgttttagt gctgggattt ttcgacggcg tgcatatcgg acaccagaaa gtcataaaga
    22381 caggtatgga aattgcacaa aaagaaggat tgaaattagc attgatgact ttcaatcagc
    22441 atccatctat tgtctttaaa aagatcgatc ctagcagcgt gaaatatttg actagcctta
    22501 agcaaaaaga agaaaaaatg gccgctttgg gtatagatta tttatatgaa gttgatttta
    22561 cttcttcttt tgctcattta gcaccgcagg aatttgttga tcaatacatc gtagggttac
    22621 atgcaaaata tgctgtttcc ggttttgatt atacgtatgg accaaaagac attgcagatg
    22681 tcgctcattt tcctgaatat gccaaaggaa gattcaaaat cgtcacagtt tctaaagaag
    22741 aagaacaagg tgaaaaaatc agctctaccc gtattcgtgg attactagac agcggacaag
    22801 taaaagaagc agcaaaacta ttaggttgca catatgaggt agaaggggta gttgtccatg
    22861 gcgatgcacg aggcagacta ctaggattcc ctacagcaaa tgtcaaagta aagagcacgg
    22921 ttcatttgcc taaagaaggt gtttatgtgt cggaaataaa aattggggaa acatggtatc
    22981 cagccatggg ttcaattgga cataatgata cattcggtga aggacgggag ctgactgtag
    23041 agttgtatat tttggacttt aatcaagata tctatggtga acatgtcgcg gtacgctgga
    23101 atcaattttt aagagatcaa gtgaaatttt ctagtgtaga ggaattgatt gaacaactca
    23161 aggaggatga acaagcaaca gctgaatatt tttctcagca ataaaacatt atg
//
LOCUS       DACDMM010000026        23065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDMM010000026 DACDMM010000000
VERSION     DACDMM010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.026"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(111..566)
                     /locus_tag="IWD16_000803"
     CDS             complement(111..566)
                     /locus_tag="IWD16_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087885.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="IWD16_000804"
     CDS             complement(720..1928)
                     /locus_tag="IWD16_000804"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9087886.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="IWD16_000805"
     CDS             complement(2040..2372)
                     /locus_tag="IWD16_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9087887.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="IWD16_000806"
     CDS             2520..3395
                     /locus_tag="IWD16_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9087888.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="IWD16_000807"
     CDS             complement(3477..4058)
                     /locus_tag="IWD16_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9087889.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="IWD16_000808"
     CDS             complement(4064..5323)
                     /locus_tag="IWD16_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9087890.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="IWD16_000809"
     CDS             complement(5325..6371)
                     /locus_tag="IWD16_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087891.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="IWD16_000810"
     CDS             complement(6606..6881)
                     /locus_tag="IWD16_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP9087892.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /locus_tag="IWD16_000811"
     CDS             complement(7124..8434)
                     /locus_tag="IWD16_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP9087893.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="IWD16_000812"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="IWD16_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP9087894.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /locus_tag="IWD16_000813"
     CDS             complement(9980..10660)
                     /locus_tag="IWD16_000813"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP9087895.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="IWD16_000814"
     CDS             complement(10709..11344)
                     /locus_tag="IWD16_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9087896.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="IWD16_000815"
     CDS             complement(11410..12813)
                     /locus_tag="IWD16_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP9087897.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="IWD16_000816"
     CDS             complement(12800..13831)
                     /locus_tag="IWD16_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087898.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="IWD16_000817"
     CDS             13862..14083
                     /locus_tag="IWD16_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP9087899.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="IWD16_000818"
     CDS             complement(14248..14985)
                     /locus_tag="IWD16_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP9087900.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="IWD16_000819"
     CDS             complement(15138..16478)
                     /locus_tag="IWD16_000819"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP9087901.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="IWD16_000820"
     CDS             complement(16715..18238)
                     /locus_tag="IWD16_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP9087902.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18616..19227)
                     /locus_tag="IWD16_000821"
     CDS             complement(18616..19227)
                     /locus_tag="IWD16_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9087903.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19350..19475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19557..20273)
                     /locus_tag="IWD16_000822"
     CDS             complement(19557..20273)
                     /locus_tag="IWD16_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9087904.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="IWD16_000823"
     CDS             complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="IWD16_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP9087905.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20832..21629)
                     /locus_tag="IWD16_000824"
     CDS             complement(20832..21629)
                     /locus_tag="IWD16_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP9087906.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21599..21970)
                     /locus_tag="IWD16_000825"
     CDS             complement(21599..21970)
                     /locus_tag="IWD16_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9087907.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="IWD16_000826"
     CDS             complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="IWD16_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP9087908.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6468 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc ccttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aatcaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagt tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa
    18541 aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca agaaatttct
    18601 tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat gttgttttcg
    18661 acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca cgatgaatcc
    18721 tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag acatgcccag
    18781 catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt
    18841 tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta aaaatttgtt
    18901 tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg taaagatcgt
    18961 tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac ctgtagtcag
    19021 cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac cgaataagaa
    19081 catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa aagccggcat
    19141 gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac
    19201 cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc ctcttcagcg
    19261 aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa atagagttag
    19321 agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac tatactgtcg
    19381 gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga ctataaccac
    19441 cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg tttacatatt
    19501 cattatacat atctattcaa aaaacgcaac atctaacaat tataaaaagt aagtgttcat
    19561 tttgagtggt tcagcaaact gctgacttct tttttgttca gttcacggta atccccagga
    19621 cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt ttagtttttg cacaggaaga
    19681 ccaactgctt gaagcatatt tttcacttga tgattacgtc cttcgtggat cgtcaattcc
    19741 acgatactgt ggtttgtctt aggatccact gataaaattt ggaatttagc tggagatgtt
    19801 tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc
    19861 ccttttacct ttgcaacgta aactttatcc acttcatgtt ttggatgtgt caggcgctga
    19921 gcaaaatctc catcatttgt cagtagcaag atccccgaag tatcgtaatc cagtcgacct
    19981 acaggataga tacgttcttt taccatcggc aaaagatccg taacaacttt tcttcctttg
    20041 tcatctgcaa cagcagaaat aactccttta ggtttgtaca ataaatagta accgtattct
    20101 tcttgataaa ttgggacttc atccacttcc acagtgtcat gtttcccaac ttttatccct
    20161 aattccctta caacttgtcc gtttactttt acacgtcctg caaggatgaa ttcttctgct
    20221 tttcgacgag aagtgatgcc agcgtgggca attactttct gcaatctctc cattacaatt
    20281 attctccttt attcattcca tcaaagaata gatctggtga cacgtcttct tcaagtgaaa
    20341 tttccatttc ttgtgtatca ggcagttctt ctaatgattt caaaccaaaa taatctaaga
    20401 agtagccagt tgttccatat aatatcggcc gacctggtcc ttctactcta cctttttctt
    20461 caatcagttt atgggcagct agacgctgta ccgctcctga aacctgaact cctcgaatct
    20521 gctcgatttc agctcttgtg attggctgtt tataagcgat gattgctaag gtttcgacgg
    20581 cggcttgaga taatgtgttt gatgtccctt gcgcatatcg cttaagtaag ggagccagct
    20641 cttttttagt cgttaataca taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg
    20701 agttttgagt gtaagattct tttaattctt tcactaaact tgtgacttct gcttcgctga
    20761 gatttaacag tccgcttaat tcttcattac tgatcccttc ctcgccaaca ataaaaagaa
    20821 gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc ctactgctgg ataaagcata
    20881 attgattcat agttttcttt ttgttgcgca atgatctgcc cttttttcat caattctagc
    20941 aaagccataa aagtagtgac aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa
    21001 gtacagccat gcgcatttct ttttatacgc tcttcgataa ataccattcg ttcttcgatc
    21061 gttgtgtcat cgggcgtgat cgttttttcc actttttgag aaaatttctt acgttctaag
    21121 attttctgca tggcacggat caaatcgatt tttttgactt gtcctcgctt caacggcttt
    21181 tcagcatccg ctaacccatc gatgttcata ggttcttttg tgtaataaag acttcgttcc
    21241 tctgcttttt ctgtcagtct ttcagctgca tatttatatt tacggtattc taaaagctga
    21301 gtgaccaatt cttctcgagg atcttcttcc cataattctt catcgtctgt gccgaattct
    21361 tggacaggaa gcaatgtctt gcttttgatt gccatcaatg tagcagccat cactaagtat
    21421 tctcctgcaa cagctagctc gagtgttttc attgtatgga tataattcat atattgctcc
    21481 gtgatcgtag cgatcggtat atcatatata tcgatttcca tttgctgaat cagatgaagc
    21541 agcagatcga gaggaccctc gaatacatct aatttaatat tgatctccgt caactcattc
    21601 actctttctt ctttttcttt gctagccatt ttccaatgta aggctctgtg atttcagttg
    21661 cacggatgga gacatccggg tacttttcct gtaattcatc taacatttga aagcccatat
    21721 tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg
    21781 cccagtaaat accaataatc ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa
    21841 gttcataatc gtcttcagat tcgtacgtat caatctcctt caacaaagaa gttttacctg
    21901 tcagtttctt atggaaactc aatagtccca tagcaatttt acggttttct ttccggtaat
    21961 gtgttaacat cttctcacct tcctatgact aagcccttgg aaaatgctgc ttataaacat
    22021 ccgtcattct tttcttcgtg atatgtgtat aaatctgagt cgtagagata tccgcgtgac
    22081 ctaacaattc ctgtactgtc cgcaaatcag ctccattttc tagcagatgt gtggcaaagc
    22141 tatgccgcag cgtatgtggt gttacttctt tgtaaatccc agcctctcgt actaactgct
    22201 tcagattttt ccagattcct tgccgagaaa gacctttccc atgattattg acaaatacat
    22261 gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc gtctaagtag cgttccagcc
    22321 attggatcgc ataatcacct aaaggaatga tccgctcttt atctcctttt ccaactgtct
    22381 gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa tccaatcaat tcactgacgc
    22441 gcatcccagt agcatacatg acttccaaga ttgcacgatc tcttatccct aacgtttttg
    22501 tcgtatcagg tgcttcaatc agcttttcta cttctgtcag agtcaatgta cttggtaatt
    22561 tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc atgatcagtg atacgctctt
    22621 gccgtaaaaa ctgatggaat cttctgagac ttgagatcat ccgagtaaca gttgctgtcg
    22681 cttttttttc ttcatgcaac ttctccagaa aacggatgac taaaaagcga tcgacatcct
    22741 gccatgaagc aattttctgc tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat
    22801 agctttgtcg tgtattggcg gacaatcccc gttcaatatt caagtagtgg agataatcgt
    22861 tgatttgctc tttcataata atccccctca tgcctctcta tcatatcaga taccgcttca
    22921 ttctgatagt ctttttccat aatctgagca aagaaaaagc cgtctagaca ctcagacgga
    22981 ctggtttctt ttttcggctc tataatttat gggactgatg aatcagaatt gattcttcag
    23041 ccccattttc attttaggta ttaaa
//
LOCUS       DACDMM010000027        22960 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDMM010000027 DACDMM010000000
VERSION     DACDMM010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.027"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWD16_000827"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWD16_000827"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP9087909.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWD16_000828"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWD16_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP9087910.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWD16_000829"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWD16_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP9087911.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWD16_000830"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWD16_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP9087912.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWD16_000831"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWD16_000831"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP9087913.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWD16_000832"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWD16_000832"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP9087914.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IWD16_000833"
     CDS             complement(8053..8523)
                     /locus_tag="IWD16_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP9087915.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IWD16_000834"
     CDS             complement(8569..8994)
                     /locus_tag="IWD16_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP9087916.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IWD16_000835"
     CDS             complement(9061..9330)
                     /locus_tag="IWD16_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP9087917.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IWD16_000836"
     CDS             complement(9341..10936)
                     /locus_tag="IWD16_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9087918.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWD16_000837"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWD16_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP9087919.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IWD16_000838"
     CDS             complement(14489..15049)
                     /locus_tag="IWD16_000838"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP9087920.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IWD16_000839"
     CDS             15402..16376
                     /locus_tag="IWD16_000839"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9087921.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IWD16_000840"
     CDS             16546..17946
                     /locus_tag="IWD16_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP9087922.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IWD16_000841"
     CDS             complement(18024..18746)
                     /locus_tag="IWD16_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9087923.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IWD16_000842"
     CDS             complement(18825..19331)
                     /locus_tag="IWD16_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9087924.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IWD16_000843"
     CDS             19454..21253
                     /locus_tag="IWD16_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP9087925.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IWD16_000844"
     CDS             complement(21321..22895)
                     /locus_tag="IWD16_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087926.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6268 a         4997 c         4201 g         7494 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat
//
LOCUS       DACDMM010000028        22268 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDMM010000028 DACDMM010000000
VERSION     DACDMM010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.028"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(130..510)
                     /gene="yajC"
                     /locus_tag="IWD16_000845"
     CDS             complement(130..510)
                     /gene="yajC"
                     /locus_tag="IWD16_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP9087927.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(607..1752)
                     /gene="tgt"
                     /locus_tag="IWD16_000846"
     CDS             complement(607..1752)
                     /gene="tgt"
                     /locus_tag="IWD16_000846"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP9087928.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1970..2668
                     /locus_tag="IWD16_000847"
     CDS             1970..2668
                     /locus_tag="IWD16_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9087929.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2836..5475)
                     /locus_tag="IWD16_000848"
     CDS             complement(2836..5475)
                     /locus_tag="IWD16_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9087930.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6094..7125)
                     /gene="queA"
                     /locus_tag="IWD16_000849"
     CDS             complement(6094..7125)
                     /gene="queA"
                     /locus_tag="IWD16_000849"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP9087931.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7318..8493
                     /locus_tag="IWD16_000850"
     CDS             7318..8493
                     /locus_tag="IWD16_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9087932.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8497..9138
                     /locus_tag="IWD16_000851"
     CDS             8497..9138
                     /locus_tag="IWD16_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9087933.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9135..10052
                     /locus_tag="IWD16_000852"
     CDS             9135..10052
                     /locus_tag="IWD16_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9087934.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10049..10720
                     /locus_tag="IWD16_000853"
     CDS             10049..10720
                     /locus_tag="IWD16_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9087935.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11261..11749)
                     /locus_tag="IWD16_000854"
     CDS             complement(11261..11749)
                     /locus_tag="IWD16_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP9087936.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11950..12216)
                     /locus_tag="IWD16_000855"
     CDS             complement(11950..12216)
                     /locus_tag="IWD16_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9087937.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12216..12479)
                     /locus_tag="IWD16_000856"
     CDS             complement(12216..12479)
                     /locus_tag="IWD16_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9087938.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(12472..12744)
                     /locus_tag="IWD16_000857"
     CDS             complement(12472..12744)
                     /locus_tag="IWD16_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087939.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            12955..13140
                     /locus_tag="IWD16_000858"
     CDS             12955..13140
                     /locus_tag="IWD16_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9087940.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13282..15105)
                     /locus_tag="IWD16_000859"
     CDS             complement(13282..15105)
                     /locus_tag="IWD16_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP9087941.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15703..18408)
                     /locus_tag="IWD16_000860"
     CDS             complement(15703..18408)
                     /locus_tag="IWD16_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP9087942.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18714..19661)
                     /locus_tag="IWD16_000861"
     CDS             complement(18714..19661)
                     /locus_tag="IWD16_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP9087943.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19851..20120
                     /gene="rpsN"
                     /locus_tag="IWD16_000862"
     CDS             19851..20120
                     /gene="rpsN"
                     /locus_tag="IWD16_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9087944.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20296..20658)
                     /locus_tag="IWD16_000863"
     CDS             complement(20296..20658)
                     /locus_tag="IWD16_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9087945.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20669..21790)
                     /locus_tag="IWD16_000864"
     CDS             complement(20669..21790)
                     /locus_tag="IWD16_000864"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP9087946.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21806..22156)
                     /locus_tag="IWD16_000865"
     CDS             complement(21806..22156)
                     /locus_tag="IWD16_000865"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP9087947.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
BASE COUNT         6305 a         4765 c         4148 g         7050 t
ORIGIN      
        1 atttatatga cacacccgat tattcaatat tgagcaatat atcccatgct cttatctctt
       61 tcttttttga ataaaaaatg aggtaggaca tctttctgcc ctacctcgtc tttaaataag
      121 ctttgatact tatttttctt ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt
      181 ttttacttca actgtctcag ctggttcttc tgttgtttca acagtagtcg ttgcatcatt
      241 tgttacgaca gtccctggtt tgaccgtttt gattgctgca cgatcgtact caaggaagat
      301 cccttcgcag tctaaaacaa ctgtccgttt gtcattgtcg atttctgaaa caactccatg
      361 taatccgccg atcgttacga ctgaatcacc tggtttcatg ctatctaaca agttttgacg
      421 ttcttgctgt tgtttctttt gcgaacgtga cataaaaaac cacattgcta atagcgcgat
      481 aaacatcaca agcattggta atgttcccat ttttttcacc tcactaattc tgtatctaca
      541 aaaaacactt tagcagaaaa atgctgataa cactagtttt ttacttaatt tttaaaagat
      601 tttcctttag aagctttttg cgttttcttt attgaacccg tattcttcaa aaaaggcttc
      661 tctaaattca agcaaattat catccatgat tgcttgacgt acttgtttca ttagattcaa
      721 caagaagtat aaattgtggt aagaagtcaa acgaatccca aatgtttcat cacatttgat
      781 caaatggcgg atataggcac gtgtatagtt tttacatgta taacaatcac atttctcatc
      841 aattggacgg aaatcatggg catatttcgc atttttcact actagacggc cttgagaagt
      901 catacatgtg ccatttcgag cgattcgagt aggcaagaca cagtcaaaca tgtctactcc
      961 acggataaca ccatcgatca atgaatcagc agcaccgaca cccatcaagt aacgtggttt
     1021 gttttcgggg atcaacggtg tagtgaattc taatacacga ttcatttctg atttaggttc
     1081 cccgacagat aacccgccga ttgagtaacc agggaaatcc atcccgacta gatctttcgc
     1141 gctttgacga cgaagatctt caaaccctgc tccttggatg attccaaata atccttgacg
     1201 gtctggatta gcatgagctt tcaatcctcg ttctgcccat cgagacgtac gttctactga
     1261 ttttttcacg tagtcgtaac tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat
     1321 atcagagccc aatttatttt ggatgttgat tgctttttct ggtgataaaa acatacgcga
     1381 accgttcaag tgatttttga aatgcacgcc ttcttcttcg atattccgca tatcacttaa
     1441 tgaaaaaact tgaaagccgc ctgaatctgt taaaatcggt tgatcccagt tcataaattt
     1501 atgcagtccg ccagcttctt ctactaagtc ttcaccaggg cgtagccata aatgatatgt
     1561 attacttaaa ataacgccag cacccatttc tttcaactct tctggggaca ttgtttttac
     1621 tgtagctaat gttcctactg gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat
     1681 caattcccct aaacgcgcgc cagtatgttt ttctttcttg attaaacgat agcgaatggc
     1741 aggttgcgtc attcatttcc ctactttcta tataaaatat cttgacaact cccaccatca
     1801 taatcgtttc aagtgctttt tgcaagttcc ctcggcttcc ttctacttta tttaatattc
     1861 ttttttttat ccttttttta tgaataacaa aaaaaagaaa aacattatga cagaaatatt
     1921 ttttgataca tggtatattg gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc
     1981 tgcagaatta aaacgccaag caaaagagag tctaaaagga cgttggggac aagctgtttt
     2041 actaaatcta atccctacat tgattggtat tgctactatt cttcttatta tgattcctat
     2101 tatcttgttt gccaccacca tatatgatgg atcagcgagc gtctcagaag cgacaacttc
     2161 aggaggcggt agttcaggag gcggtggcgg aattatttcc agcatcatta gcgctttgtt
     2221 catgagcggt atttcttgga cttatcttga tattttacgt ggacaaaaag atcagatcga
     2281 accattcaaa gatgcatttc gtggattcaa aggtttgttt atcggcggag tgatcagctt
     2341 agctgtgctg atctcgatct ttacaacatt atggggtttg ctattagtta ttcctggaat
     2401 cattaaaagc tattcttatt ctcaaagcta ttttatttac tacgatatcg tccaagaaac
     2461 aggacaaaca cctaaagtac tggatacgat cactgcaagt cgaaaattga tggatggtta
     2521 taaaggacaa cttttctggt tagatctttc atttatcggc tggcatatct tagcattagc
     2581 aactgctggt attggttatc tatggctcag cccatacatt tctgcaacaa aagcagcatt
     2641 ttatgaacaa ttgccaaaag atatttaaaa gagagtgtga cttgtgaccg agggggtggc
     2701 aggagaacac cgtttatcga gaggcagtaa aagaaacatt cagcccatta gcaaaaagga
     2761 ggtgtgacaa cattcatgtc acacctcctt tttgtatagt tccagtaaaa agaaaatcaa
     2821 cttagaaaca gttaatcatt cctcttcttc tgcttccggt aatcgatgga acttgatcag
     2881 ttcattcacc gcgattggta cgatagataa aatccctacc aagaaccagt gattcaaact
     2941 gatgtgtact aggccgaata gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat
     3001 agctaatgcc agtaacgcca tttctaccaa agctttattg gtcgctaaat tgactttgaa
     3061 gatcgattgg ctgctgcgta cattgaacac atgcagaatc gatgaataag ctaagatcaa
     3121 gaatgcaaca gtttgaccga catgctgact tgcgtctacc catgggctga tagtatcgac
     3181 ataactacct aaataataac caaacaaggt tacgatggtg aagacccctg catttatggc
     3241 aattttttgc aataaacctc gagcaaagat cccttcattt ttcggaatgg gtggctgatc
     3301 catgatgccg tgttccgctg gctctcttcc taaagcaaat cctggaagtc catcagcaac
     3361 tacattgaca aacagtagtt gcacagctgt aaatggtgct ccccatccta acagcatcgc
     3421 aatcaaaaca ataaagatct ccgaaatatt acagcttagc aaaaagttga tagctttacg
     3481 aatattctga taaaccgaac gtccttgact gacagcgtct acgatcgtcg caaagttgtc
     3541 atccgtcaaa atcatatctg cggcactttg cgccacatct gttcctgtga tccccatggc
     3601 acacccaacg ttacttgctt tcaacgctgg tgcatcattc acaccatccc cagtcatcgc
     3661 tacgactgca cccgtacgtt gccaagattt gacgatccgg atcttgtctt ctggcgttac
     3721 ccgagcatag acagagagag ctttgacacg cgcatctaat tcttcgtctg ataattgatg
     3781 cagttctgat cctgaaagtg cttctgacgg atcattcaag atacctaatt ctttcgctat
     3841 ggcactggca gtcaccacat gatctccagt gatcatgact gttttgatgc cagccttttt
     3901 cgctcgcttg attgctcctt tactttctgg acgtggtgga tcgatcattc cgatcagacc
     3961 aatcaaacgt aagtcttttt ccagtgcttc agaagaaata tcttgcggtt cttcgtcata
     4021 agtagcataa ccaacagcaa tcactctcag tgcccgttta ccaaagcgat cattaacgat
     4081 cgccgcttgt tctacatcgc ctgaactaaa gcgcggagca agaacatcga atgctccttt
     4141 ggtgattgag atgtattttt cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga
     4201 atcaaaagga agctcgttta ctcgtggata tttttcttct aattctgcct ttgtatgata
     4261 attctcttca acagccctta cgatggctac ttctgttgga ttacctttga taacaaggtc
     4321 gtcatcttct tgctcgaccg tcacatctgt acatagtgca gccattttca atatttccat
     4381 cgcgctgttc gtcattgaat cttcgatatt cgttacttca tcttctttgc tccatacccg
     4441 gcggacgcgc atttcatttt gtgtcagcgt tcctgttttg tctgagcaga taacattagc
     4501 ggttcctagc gtttcaactg ctggcaactg tcggatgatc gcatgtttcc tcgccatctt
     4561 ctgcacaccg tatgcaagtg tcaacgtaac aataacagtc aaggtttccg gtacagctgc
     4621 aacagccaag gaaatcgaag tcatgaacat ctcaaggagt ggttctcctt gcagttcacc
     4681 aatcacaaat actaaagccg cagccgctaa agcgatcata ctgattcgtt tgcctagctg
     4741 attcaatcgg acctgtaaag gtgttttttg catagtattt tcgttcaaaa gatccgcgat
     4801 cttacccatt tctgtagcca tgccaatggc agtgacgatc gctttcccac gacctgcagc
     4861 aaccgtacat cctttaaata ccatattaag ttggtcacct aatgactcat cttcttcagc
     4921 aataaatgct gcatcttttt caactgcttc actttctcca gtcaacgccg attcttctac
     4981 acgtaattgt gatgcttcga tcaaacgggc atcggcagaa atagcatcac cggactctaa
     5041 aacaagaata tcacccacta ccagttgctc tgactcaaca gtggtcactt tgccgtctcg
     5101 tatgacagtt gccgtttgtt tattcatatc ttgcaaggat tccagtgctt tttctgcgtt
     5161 tccttcttgg acaatcgcca ataccgagtt gatcacaacg atagcgatga tcagcagtcc
     5221 ttcaaataaa tccccatgtt ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac
     5281 aagaaggatg atcgttgtaa aatcggacaa gcttcgaaag aattttttga tcatcgattc
     5341 tttcggcgcc tcatcgaatt tattcaatcc ctgctttttt cgccgttcat ccacttcttg
     5401 tgaagacaac ccagtatctg gatctgcttc tgttaccttc tttacttctt ctattgtctg
     5461 cttgtattct tccatcctat tcctccttta aaatcttcgt acaaaaaaag gtacttattt
     5521 tactataaca tccttttttt atttacttca aataaagggc ttatcgatta tttgttaaga
     5581 aagcgtaaaa tttaaagtgc ttatatttgc aaacgctacc aaaggatgct attatataga
     5641 tgtaaggaag gaggatacca tcaaatagac agaagaattt ctcgatagaa cagtgtaagc
     5701 aaacaaagtt gttagaagta caggtttcac ccagacatgc gtgtagagta caaacctata
     5761 aatagtgaat gcgtatggat gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat
     5821 acgtaacaat tgaataacgt aaacgtctga aaattgaata ggaacaagag atgtttcaaa
     5881 tgttgaagat gtaacgaaaa caaaattttt gtttaacatg aagaaattca acaccgtgct
     5941 ttgcatgtat gatggaaagt tacaatcatt tctgtttatt tgaatccttc cgaggtacaa
     6001 gaaagccgag cttatcgaga gctcggcttt ttctttgtca taaaatcttt gtaaagcttt
     6061 tttgttattc aaaactagcg gcttttcata accttatttc acgaacatcg catctccaaa
     6121 actaaagaaa cgatagcgct cttcaatcgc atgctggtaa gcagataaag tcaattctct
     6181 tcctgcaaag gcactaacta acatgactaa agtcgatttt ggcaaatgga aattagttga
     6241 gaaagcttct accactttga attcatagcc aggcgtaatg aaaatatcag tccatccgct
     6301 gtctgctttg atttctccat taaatttcgt tccaatcgtt tctaatgttc gaattgaagt
     6361 tgttccgaca gccacgattt tcccgccatt tttacgtact tggttcaatc gctcagccgc
     6421 ttcttccgtc aaacggtaga attcactatg catatggtgt tcttcaattt tatctacgct
     6481 gacaggtcgg aatgtaccta accctacatg tagtgtcaga tagactaatt caacaccctt
     6541 tgcttcgatc tcatcaagta attctttcgt aaaatgcaat ccagctgttg gcgctgcagc
     6601 agaaccattt tctttcgcat agacagtttg atagcgatct gggtcttcca gacgttcttt
     6661 gatatatgga ggcaatggca tttctcccaa tgattcaaga atctctagaa aaattccttc
     6721 atactcaaag tcaatgatcc gtccgccgtg ttccaattct tctttcacta ctgcttgcaa
     6781 tctgccatca ccaaaagaga ttcttgtccc aactttggca cgtttcgcag gtttgatcaa
     6841 tgtctcccac gtatctcctt gtgtattcgt tagaagaagg acttctaaat gcgcaccagt
     6901 atctggtttt tctccatata aacgagcagg cagaactcgt gtattattca taactagcgc
     6961 atcaccaggc tgtaactctt caatgatttc atggaaatgt ttatcttcca tctctccagt
     7021 tttccgatcc aaaacaagta aacgagaatg atcgcggtct tttaatggtg tttgtgcgat
     7081 taattcttcc ggcaagtcaa aatcaaaatc ttctgttgtt aacatatcgt cactcacttt
     7141 ctatcatgtg cttcatcatt ctatcatgtt tttcgttctg ttggcaaaat cagcactttt
     7201 tcacttctat tttttgtttt cctaaataaa aaaactaccc tatttgtatt ttttcattgt
     7261 tttaggcata ctagtaatag atagttcttc attgaaaata gaaaaagagg tggcaccatg
     7321 attgaatttc aaaatgtgtc aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc
     7381 aatctttcat ttgaaaaggg tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa
     7441 acgacgacga tgcggatgat caaccgaatg actgatccaa cacaagggaa aattttgatt
     7501 gatggaaaat ccatccaaga aattgatcca gtggaattgc gacgtcagat cggctatgtt
     7561 atccaaagta ttggattgat gcctcacatg acgatccgtg aaaatatcac tcttgtccaa
     7621 aaattgttga aagtcagtca agaagaacgg aataaaacag ctgaaaaaat gattgatctt
     7681 gtggaattgc cccgtgagat gttagatcgc tacccacatg aattatctgg tggtcagcag
     7741 cagcgtattg gagtcgttcg tgcccttgca gcgaatcaag atatcatctt gatggacgag
     7801 ccattcggtg cgcttgatcc aatcactcgt gattccttgc aagaccttgt caaagattta
     7861 caagagcgtt tgggaaaaac gatcgtattt gtaacccatg acatggatga agcgattaaa
     7921 ctagcaagcc gtatcgccat catgagtgaa ggccgtgtga tccaatttga tacaccagaa
     7981 aatattttac gtcatcctgc aaacgatttc gtagaagagt tgattggaga agatcgccta
     8041 ctacaagcca agccaaatgc gacaagagtc ggcgaagtta tgctaaatac agcaatcacg
     8101 attacaccgg aaaaatcatt acaagaagca atcaaactga tgagggaaaa acgtgtagat
     8161 acgttgttag ttaccgataa ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg
     8221 aataaaaaac gaggaaaagt atccagtgtc ggcgacattt tgaataaaca tgttttttac
     8281 gtaaaagaat ccgcctatct tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa
     8341 tacgtgccgg tcgttgatga acagaacaaa gtcgtaggta tcctgactcg tgcatcatta
     8401 gtggatatcg tctacgatgt gatttgggga gaagatcccg caactactga tgttgcagag
     8461 tcagctactc ctgaagaatc taaggaggtc tgacctatgc agcagttttt tcatcagcac
     8521 ggttcagaaa ttctctcaaa aggttttgaa catctttata tttcaggact tgctttgatc
     8581 attggtatca tcattgctgt accgcttggt gtcttactga cacgttttcc aaaagctgcc
     8641 acgattgtga ttggcttaac cagtgccttg cagacaatcc cttctcttgc tttattggca
     8701 ttgatgatcc cgctatttgg cgtaggtaaa ttacctgcga ttatcgcact ctttatttat
     8761 tcattattgc cgattttacg taatacgtac attgggatga aaaatgtcga ttggaattat
     8821 cgggatgtgg caaaaggcat ggggatgact gagttccaat ccattttttc cgttgaattg
     8881 cccattgcta tgccaacgat catggctggt attcgtttag ctgccgttta cgtgattgca
     8941 tgggctacac ttgcctctta tatcggcgct ggtggtttag gcgatttgat ttttagtgga
     9001 ctgaataatt accaaccaga tctcattttg gcaggtacga tccctgtaac aattttagca
     9061 ctattggctg attggcttct aggtctcttg gaacaccggc tgacaccgat cgcacttaga
     9121 gaggagaatg aagaatgaaa cgtatactaa aaatgaccat ggttcttttc tctcttctta
     9181 tacttgcgag ttgttccttc cctggtcttg ccagcaatac agatgaagac accatttcga
     9241 tcacaggtgg aatcacttca gaagcacaga tcttagcaag tctagttgcc ggaatggttg
     9301 aacattatac agataaaaat accgcaatca tcaacaatct ggctactaca actatcaacc
     9361 atcaagcgat gatgaacgga gatgcttcaa tttcagcggc tcgctacaca ggaacagatt
     9421 taacgactac attgaacctt cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga
     9481 aagatgaatt tgagaaaaga tacggtcaaa cgtggttccc gtcctatagt tttgaaaata
     9541 cgtatgtctt tcttgttcga aaagacaccg cacaaaaata ccatctatct aaagtcagcg
     9601 atctgaaaaa tgtagcggat gaattagtag ctggtgtaga tacttcttgg atcaatcgta
     9661 aaggagacgg ttatgacggc ttccaagaaa cttatggctt ctccttcaat tcgatattac
     9721 ctatgcagat tggacttgtt tatgacgcag tcgaagcagg aaaaatggat attgttcttg
     9781 gctactctac ggatggacgg atcgccagtt atgatctagt catgttggaa gatgatcgtc
     9841 aattcttccc accatatgat gctgctccag ttgtagacaa caagatatta gctgatacgc
     9901 ctcatttaaa agaagcctta aataagttag gtcataccat cagtacggaa aaaatgcagg
     9961 aattgaacta cgaagcagat aataatctag tggaaccatc agtagtagca caacgtttct
    10021 tacaagaaaa ccactatttt gaagggaagt gaagcaattg gataaaatga atgtctttca
    10081 acaatttttc tattatttcc aggaaaatgg ctcctatatc tttgcacaat ttatccgtca
    10141 cttcctgatt tctatctatg gtgtcctctt tgcagcagtt gtcgggattc cggttgggat
    10201 catgatttct aggagacgta aattagcaaa ctgggtgatt cgtttggcga atatcatcca
    10261 gacgatccct tctctagcca tgatctccat tttaatcatt ggattaggcc tgggagtcaa
    10321 tgtcgtgatt gttactgtat ttttgtattc cctcttaccg atcatcaaga atacgtatac
    10381 cgggatgatc caagtagaca aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc
    10441 tagacaacga ctattcatgg tagagttacc actctctgtt tctgttatca tggcaggcat
    10501 aagaaacgcg ttagttgttg ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg
    10561 tggtcttgga gatatcatta tccgaggaac caatgcaaca gagggcacat cgattatttt
    10621 agcaggtgca ttgccaactg cattaatggc gatcatcacg gactggcttt taggtatttt
    10681 ggaaagacgg ctagatccag ccagccgtac atcacgctga tgttattgta aaaaacaaaa
    10741 gcttctgcga ccatttgttg gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat
    10801 aatccactct gattcttccc tccttatggg taaagagttg cttttcttag ctatttatcc
    10861 attccgtata ctccaacttc atactgccaa cgggttcata tctgcatcca ccgactaagt
    10921 ttttttctta tccgctaagg cattttgcca atccattttc ccatcactcg tcatggaatt
    10981 cataccacat gatctctaaa tactcgtctg cttgttcttt gaattgggca tcaattctta
    11041 catcctttgg cgtggctttc atttaagcct tttccatcag tcctcgattg ttcgatgcct
    11101 tcagctgcat catcaaaact ggtgactgcc gtatcggtaa cttcgatcat cttttataca
    11161 atcgggaact tgccatcctc atccttggct aaaaacatat ctagccattc cagcttctcg
    11221 ccatgggagg tttgcggttc ctaaaaccgg tacacgattc ttatggactg catccgtcgc
    11281 aactagactt ggcgatacga tgatccttca ccagaagagc acccccgtaa accaattgat
    11341 cgatatattg ccaataagaa aagacattgg cgtaaaagat atcgatccct cgtgatgcat
    11401 tgccgttgat actgctattc atggccgaaa gcgccacgac tttaacttct tccgtttgag
    11461 tgacattcat gttcggcaat gtcgctttgg atacccgctc tgctaaagga acgacactgg
    11521 tattgtattt cgtatctgta tcttttccaa aagaccatgc caacgaatcc tctgggaacc
    11581 aataagacga ctccggttga ttgtccattg cttttttcaa catacttttg gctgtttctc
    11641 gataaacgac atttccaatt gtcttcgcct cactttggcg ataatcgatg gtcgttgcca
    11701 ctgccaacac taacaacgat gctccaattt tactgatttt ccttgtcaca cttcttcctc
    11761 cttgttttac ataaacccgt caacattcca ttcctaaact caaacaatga cccttcaaag
    11821 gtggagatat aggaacaaat taaagaaatc atgcaataaa tccaagaagg ttggttttag
    11881 aacaaaaaaa gagtctcctt tttggggaga ctctttgtta actatctaaa aaggttggct
    11941 cttctcactt cactcataat gtgtccaggc agaataaatt tctacaactt tattttcctc
    12001 atcaactttg taaaccactc gatgctgtct attcagcctg cgtgagtatc tgccttcatg
    12061 tgtaggtctc agcttttcaa aagactgcgt gggcatatat ggatcttcct tcaatgtctg
    12121 aatcacttct tcaaattgtt tctttaaatt cgtttgcttg atttttctta aatccacttt
    12181 cgcagagttt ttgattgcga ctgtatagtt actcatcaaa gagcttccca atcaatatca
    12241 tcgatattcg taaagctact gtcgtctttt tcgcggtctc gtactttatc taaagtacct
    12301 gtttgctcaa gaaaaagggt ttcttgaatc gctctccagt cttccagccc aatcaataca
    12361 gcattgtttc cactacgatc actaataatc tcgatttcag tatgattttc atttacctct
    12421 ttcaataact gataaaaatt tttacgagca gtgctaggat tcatcgttgt attagccatt
    12481 gtctctcctc ctttttctga tataattata gcgtacttga ttaagtacgt caaaaaaata
    12541 caataaagca cttaaattca gcaaactttt cctctttttt acatagagaa aaagacccct
    12601 ttttagggag gatctttgtc cacgctcact tcttactcct ttacgactag cggtagccaa
    12661 acgtaaagta attggtaaca acgtatcatc tttagcaaag gtcgaatcca aagcctgtga
    12721 gtctatgcaa caacaccaat acacgaaggt tcctcgttgc agtgtgactt gattgatatc
    12781 ttgaataacc tgaggatacg cacgaaagat ttgtaacggt tattcaaaac tgattttggc
    12841 cgtcaagtct tctactgcct caaaaatgat tgcctctttc gtctcctgtc attcatgttc
    12901 actatcgatt tgatacttgc ttagaccgtt gggtttgtga atcaccaatt aaaaatggca
    12961 ctgagttatg gtaacaaaaa atggtcactt gacgaggctg agattcctac ttatgaagac
    13021 acatggcact taagagtcga ggtaaaggaa gaagctgaaa atataggagg ctgtacatta
    13081 tttggcaaag gattcggaaa ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa
    13141 ataggaagaa taataacatt tcatacgctg ttcccatctt taatggctta tagagggcac
    13201 ctttagctga gaataagaca aaacagaccc tctagcttca attaagagct agagggtctg
    13261 tttttgattt taaaacttgg tttatttctt caaatggtag ctataggtgc tgacaacgat
    13321 attttcacgc caagacaatc gcaatcttaa ccgcaaactt gtttggttat ggagaatatt
    13381 ctgccagcgt ctgtttggta caaattgagg aatcaacaca gtagttgtat agttttgttt
    13441 cgctgcattt ttacttacga tatctacata acggataatt ggattttcaa tcgagcgata
    13501 agaagagtgg acgattgaaa aacggacatc tgggaaatgc tttttaaatt ctgcttcgat
    13561 ttctttttct ttttcgacat cttcgtctag cgaaacatgc atcgcaacga cataatcacc
    13621 gatcgaacgt gcgtaattca acgcaccgat atttacccgt gtcacattcc cgactaatac
    13681 aagaacggta ttcccgtcat aatcatgtag ttttgcatca ttttctaaac gaagctgttc
    13741 tgctactttt ttataatgat catggatctt ataaaaaatg aacataacga ttggcataat
    13801 gatgaagaaa ggccaaatgt cccctaaccg atagacaaac aaaatcgcaa tgattgcata
    13861 tgagatgaac gcacccgtaa tattggcaat cgatttgctt aaccatcttt ttccttcttt
    13921 tttccatttg acgaccatcc ctgtttgaga taaagcaaat ggaatgaaca cgccgataga
    13981 gtataacgga atcaagcgtt ctgttgagcc atggaaaata aacagcaata cgatcgaacc
    14041 tagcgctagt gtgataatcc cattggaata gcctagtcga tccccacgat cttgatacat
    14101 gtgaggcatg aatttatctt ttgccagatt gtatgcaaga actggaaaag cagaaaaacc
    14161 ggtgttagca gcaactgcta aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat
    14221 accatgtcca aagacagctt taccaatttg agacaaaaca gtgacttctt tttcaggaac
    14281 gatcccatac caatagttga taaatgtgat ccccacgaag aagaaaccta agatcaatgc
    14341 catcatcgtc aatgtagcag cggcattttt tgctcttggc ttcttaaaga atggtacagc
    14401 attactaatc gcctccaccc cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat
    14461 caaagcgata gaaacacccg gcacagccgc tcctggaagt gcagtagctt gcaatggcac
    14521 agcacctgtc acgatattat ataaaccaac aacgatcagt aacgagatca caaggataaa
    14581 tgtgtacacc ggaaataata ggaacgatgc agattccctc agcccacgaa gattcagcat
    14641 catcagtagc aaaacgattg tcacagaaat agcgacttga tgaccgtaga gtgctggcac
    14701 cgctgatgtg atcgcttcag cacctgctga gacagatact gctactgtca acatgtagtc
    14761 gatcaataaa gagccaccgg aaatcaagcc agcattcttt ccaagattct cactgctgac
    14821 gacatatgct cccccgccat gaggataagc atggatgatt tgacgatatg atagcgttaa
    14881 tgaaatcaat aaaataataa caaaagcggc aatgggtagc gaataccaga tggctgcagc
    14941 agacaaagcg actaaaacga caacgatttg ttccgtacca tatgcaatag atgataacgc
    15001 atcagacgac aataatgcca aagcagcaaa tcgggttaat ttgtgctcat cattttcagc
    15061 agattttaaa ggcttaccta ctaacaacct ttttaagtaa tccaatttct aaactccttt
    15121 acacacaatt ttacttcaag ttttatagtc gaaaatttat agtaaacaag atgttatagt
    15181 acgctcactg accttaaaag tcaataaact ttaagaaaat aattggaaaa aaacagaaaa
    15241 atatcaagcg ctttttcaaa cgctttaata cagcaaaaat ctaacatttt tttatgggct
    15301 ttttctttga attctataat aaaaggatag aacttgactt agaaaatcaa gttctatcct
    15361 ttttgatatc ttaacttttg gaagagagat gtaaggctgc tttctaccca tcattcttac
    15421 tactcgaagc atagcgcagt tgtcacaacc tcttgataaa cggtgtttaa aagcttgcat
    15481 ctgcggtaat aagcccaacc aaattaaaaa cataagaagc tgtttttatt tggtcgttct
    15541 tattacttga tgcagagcag cttttttaca acctcttggt acacggtgtt caatcagctg
    15601 accctcggta ttagctcaaa aaaacggcaa aatatgagga actatttttc catttttttc
    15661 tctaatactc aggtcaaaac actgatttca caacctcttt ttttaatgaa aaatatggct
    15721 ctcatgcgcc gcttctttga atttcgtcat tttttcttcg atgaacggat agattgccgt
    15781 tcctacgatt ccaaagacaa caaacaaacc gatcatgatc cagattgctt tcgtagcatt
    15841 tggccaataa attcctcctg cagattcccg taacaagttt accgcatgag taaatggcaa
    15901 gaacggattg atcatttgga agaatttccc tgagacttgg atcggatagt tcccgcctcc
    15961 gccagagatc gacagtacta agatgataat agcgatccct ttaccgacat tatcgaataa
    16021 tgcagccaag acatagacga tcatcatgaa cgctaaggcg atcaagacag caaatgacac
    16081 gctgtagacc ggattttgga catctactcc tagtaagaaa atattcccta atgtaacgat
    16141 caagctttgc gcaatcgcca ttgtcaaaaa tgtcagcata cgagcaacga attgttctcg
    16201 tttcgtaaaa ttgtttcggt ctttctttcc taaatagtat tctgtcgtag caacacttga
    16261 aagaagaaca gcgccgaccc acagacatag tgcagtataa aacggggtgc tagctgaacc
    16321 gttatttgcg attggataaa gcgcattggt ttgtagctca acaggttgtg taaagaagtc
    16381 actttctttt gttgcgtcta gtttcaataa tttgatgact tcgcctagat cgacttcttg
    16441 ttcgcctttt cggatcgcgt ctgctgcttt ttggatacca gctttgatat ttggccagtc
    16501 attgttgatc aagtccgcag cagcgttcac agctgtttcg acttctggca ttttttcatt
    16561 tactgttgtc aatgtaccgg tgatttcatt ttttagtgct ggccaatcat tttggataaa
    16621 atcagcggcc aatcctaatt tttgctgaat gatcggtaat tcattgttgt ataggtctgc
    16681 cccttcattg atgccattga cgatggtatc catatttcca ttcagcattg tattggcatc
    16741 atggatttct tgtccaattg caggcatttc tttttgatat ttttctaaaa ttgccactgc
    16801 attcgttaca gttgctttcg ttgagctgag aagcgaatca aagtcgatct ggtccgcttt
    16861 gctcagtgcg ccttgtgcag tcccaatcgt atcgatgact tggtctagta gagaagtgac
    16921 agtggaactg atttgacctg tattgatacc agcaatgatg ccattgatat tattaacaac
    16981 ggcatcaatt tgatctagca attcatcaat agccttcgtg tcttcatttt gaacagctgt
    17041 gttcaaagca gataggcggt tttgcagatt gcttagcaag ggttttaatt gatttagctg
    17101 atcaataatt ggctgtaatt tcgtatttcc agcacttgtt tgcaatttag tcaggaactc
    17161 gaccagttta tcgattgcat tttgttgttg agatattccg tcaataaagc cgttcaatac
    17221 attgctgatg tttttctttt catcatcagt cagcttacca tcttcaatag ctgttcttac
    17281 agtatctatt aacgaacctg tcgttgttgc cacttgtcca attgacttca atgtaacatc
    17341 tacactgttt gtgatgcttg gtatcgcatc tttcagctga gaggcacctg attttgtttg
    17401 gtttgctaga tcacttgcct gattttctaa atttttcaca tctggcaaga tatcttgtac
    17461 ttgttggatg atcgtcaagc cttgttttgc ttcatcgatt ccttggttca ttgtctcttg
    17521 gatcgaagca aaatcgccgt cgatctctgc tagttgctta ccggcatttt ggatctctgg
    17581 gattttttct tgcaaatcaa ggatgatttt tgcttgatct ttcaactcag gcattttgtc
    17641 attcaatgca acgacttttt cacccatctc atctacttta gggatgtaat ccacgaattc
    17701 atttgcttta ttcagttttt ccttgatttc aggtagttga ctttgtaatt cgaggacttg
    17761 ttttgtgtag ccgtcgatcg tgtccaaatt ttcatctgtt gataaaatca tatctttaac
    17821 tttattgata cttactaggt tcgtatcgat gtcgtaacct atctcattga atactttcaa
    17881 taaagtcgaa ctggctgttt tgataaattc atttgtgatt tgcgattgga tcgaacttgc
    17941 tcctttatcg gtgattttcg gtgcaatcgc attgattttt tcatttaccg tatattcgat
    18001 ttttggtttc ttgatatcac cactagtaaa actcagcaga tcttctgaga aatcctttgg
    18061 caagtaaata cctgcgtaat actttccgga acggactccg tcttctaatt gttcttttga
    18121 atcaacgaat tgccatccta attgtttatt tttatgaagc gattcgatga cttgctcccc
    18181 aatggccact tctttacctt gaaactctgc tggcttgtct gcactgtaaa cagcaattgg
    18241 cagctctcca gtatttccat atggatccca taatgctttg atgttaaacc acgcatatag
    18301 cgaagggatg atcatgatcg cgatgatcag aagtgttgca atcgggtttt tgaaaatccg
    18361 tttccagtca agcaaatata atcgaagcgt atttttgatt gactgcatag ttctccccct
    18421 tcttcttttt tcagctgaat tactttacca tgtatttcat acttttttca atcggctttt
    18481 ttcaaaaaag tcgaattttt ggacatatca cggcaattgt ctcattgtaa tctcttacat
    18541 attgagaaat aattaaggat tacagccttt actttatcat gaaataaaag ggaaagataa
    18601 ttttatgcca aagtttttat ctattatacg tatacgcaaa aaagctaaaa cattttttgt
    18661 ctttgcccct acaaaaatgt ttcagctttt ttcgtttttt attcctgtat ttcttatttt
    18721 tcgaatgaca ggatgatttt cccagcagga ggatgtcctt caactagctg atgtgcttgg
    18781 atgacatgct taagtgaagc agacagattc attgaaataa aaacatgaaa ggctcctttt
    18841 tggtaggctt tgattatgcc agcaaaggct tctgcatcta gatattcctt tcttggaaca
    18901 aacgattcat aaaagccttc ctttttttgc cgcaaatcta gatctgggag gtcatttaat
    18961 gcaacatacc tgccgcctgg tttcatgatc tggatgccgg tttctccttt gatacttcct
    19021 ttcgtcgcat cgattacaag gtctgcctga tcagcaaatt gcagacctgg atttgtttta
    19081 tcataagcag caaatgctga agctcctaat ttttttacct tttcctcatt tttactggat
    19141 gcactggtta agattcggat tcccttttca tggagaagct ggatcagact tgacccgacc
    19201 gcacctgatg ctcctagtat cattacagta tctgtcggct ggatttccgt caaatggttg
    19261 attagattat aagccgtaat tcctggcgtg accattccgg ctgcttcttc ccaactcatt
    19321 ttatctggaa tttttgctac ttttgctgat ggcaacacga cttcttctcc gtaagtcccg
    19381 ccgactgcat ggacagctac tcgatcacct acatgaacag ttgtgacatc tgaaccaatc
    19441 tctgtcacaa taccagcgcc atcattacct ggtacataag ggaatttcaa tgttcgtatc
    19501 tctttcattt ctcctaaacg caaggcaaca tcataaggat tcacactgaa tgcttttatt
    19561 tctacacgaa catgtccaga accgacttct ctgggatgag catcgatttc ttcaaaaaca
    19621 tcttgtgctt ctccgtaacc gttaattgca aatcttcgca tggtcaccat ctcccttttt
    19681 ctttaagttt acgtttatgc ctttattttg acaaaaaaaa tgtcttttcg ttttggcgca
    19741 tgattcaaaa caaaatagtt gtcttttttc gcgtaacgtg ttaaattatt tatctaaaag
    19801 cgtaaagatt acgatttacg ctaataaaaa ataattggag gaatttgttg atggctaaaa
    19861 aatcaaagat tgccaaagca aaaaaacagc aagaaacgat tgaacgctat gctgctttac
    19921 gccaggaatt aaaagcgcaa aaagattacg aaggcttgcg taaacttcct ttagatgctc
    19981 gtccagaacg attgaaaaat cgtgatttaa tcgatggacg ccctcgcgga tacatgcgta
    20041 aattcgggat gtcccgtata aaattccgtg aattagcaca taaaggtttg attccaggag
    20101 taaaaaaagc cagctggtaa tccaagtctg gctgtaatgt aaaggaggag ggcgcatgga
    20161 ccactccttg gatcatcaaa aaagatacgt tcgcttcttc tagcagaaat gctggtacta
    20221 gaagcagacg tatctttttt tacgcacagc tggtcctttt ttatatgtaa aggctgcatg
    20281 gttacttcgc tatatttatt tagtaacagg agtcagtgct tcgataccca cactaatttt
    20341 caatgctcgg tcaacttctg ccataatggg tcgatccaaa tggcagacct tttctttcaa
    20401 tcttgattta tcgatcgttc tgatctgctc caacaggata acggagtctc tttctattcc
    20461 tgttccagta gctttgatcc caatgtgagt cggaagctta ggtttagcca tttttgccgt
    20521 tatggccgca acaatgatcg tcgggctaaa atggttacct agattatttt gaataaccaa
    20581 aactgggcgt gttccgcctt gctccgatcc cacgactgga gaaagatcag caaaataaat
    20641 atctcctctt tttaccatag gatcctcctt attcttgata ttctcttggt atccgctgtc
    20701 ctaatccgca agccacttca tagtggatcg tacctaattg atctgctata tcctgcatag
    20761 tgatttcttt gtctgcattt ttgccgataa gcgtcacttt tgttccgaca ggaaattctt
    20821 gaggcaatcg gatcatcagt tgatccatac atacacgccc aatcgtttca cagtagttcc
    20881 cttctaccaa tagagaaaaa ccctgcatct ttcgtggcca gccatctgca taaccgatcg
    20941 gtatcgttcc gatccactct gcttcaggag tgatatatgt ttctccatag ccgattcctt
    21001 cacctgctgg gagttttttg acttgaatca attcagaaac cagctcaagt gctggctgca
    21061 atggataaac ttccggtagt gcgtgcccag agggattaag tccgtacatt gctactccat
    21121 aacgaatcat attgccgatt gtttcatcat gccataaagc cgttgcacta ttgctgacat
    21181 gtacatatcg aggaagttcg ggaagttttt tcagcacttc tataaagcgt tctttttgca
    21241 gattccaata tgtatcatct gcctgatctg ctgtagaaaa atgggtaaat aaaccttccc
    21301 attctaatac acgactttct ttgatgaatg cagctgcctc ttttacttct tcaggtgtac
    21361 aaaatccgat acgacccatc ccagtgtctg cttttagatg aatcgaaaga ggcgtctcta
    21421 gcttactttt ctctaaaaca gcagctgctg cttgcaacca ttcccgtgta cctgcagtta
    21481 ctgataaatc atgagcaaca gctacagtca gtgcgtcgta aggaaccata ttcaagataa
    21541 ggatgggctg agtaaatcca gcttctctca actcgactcc ttcatctaaa tttgaaacac
    21601 aaaagcccga agctccacct gcaactgctg cctcagctgt ttcaatagct ccatgaccgt
    21661 atccgttcgc ttttactact gcaaacaatt cttttccttg cggaagtctt gcaacttcat
    21721 ttgctacatt ttcagttata gcttttttat gtatcactgc tttcgttggg cggtgccaag
    21781 cgacaaccat cgtttttcct tctttctagt cttccaaaat aatttgtgca aaagctgttt
    21841 catttgtatg agtaatactg acccatacat tgccttcatg aggtgaacgc gtcacaacag
    21901 gctgaccatt ttcatttttt aaaatctcga catcttgaaa ggtaactttt ccgatcccag
    21961 ttccccaagc tttagagaaa gcctctttgc aagcgtaacg cccgcctaaa aattctactt
    22021 ggcgatgaaa aggcaaggac tggaatagtt tcatttcatc agaggtcaag attcgtgcaa
    22081 tgaattttgg tttttcttcg atcagccgag tgatcctagg aagttcgact gcatcgatcc
    22141 caattccttt tatcatatgc ttttctcttt tcaataaatt tagaacactc tcattctacc
    22201 aaattataac cattttgcca caaataagtc cacaaataac aaaaatgaaa gtttctctta
    22261 aaaaagag
//
LOCUS       DACDMM010000029        22214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDMM010000029 DACDMM010000000
VERSION     DACDMM010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.029"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            82..1980
                     /locus_tag="IWD16_000866"
     CDS             82..1980
                     /locus_tag="IWD16_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP9087948.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2279..2998)
                     /locus_tag="IWD16_000867"
     CDS             complement(2279..2998)
                     /locus_tag="IWD16_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP9087949.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            3335..3667
                     /locus_tag="IWD16_000868"
     CDS             3335..3667
                     /locus_tag="IWD16_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087950.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3654..5657
                     /locus_tag="IWD16_000869"
     CDS             3654..5657
                     /locus_tag="IWD16_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP9087951.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5676..6146
                     /locus_tag="IWD16_000870"
     CDS             5676..6146
                     /locus_tag="IWD16_000870"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP9087952.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6167..6751
                     /locus_tag="IWD16_000871"
     CDS             6167..6751
                     /locus_tag="IWD16_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP9087953.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6766..7752
                     /locus_tag="IWD16_000872"
     CDS             6766..7752
                     /locus_tag="IWD16_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP9087954.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7742..8053
                     /locus_tag="IWD16_000873"
     CDS             7742..8053
                     /locus_tag="IWD16_000873"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP9087955.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8075..9856
                     /locus_tag="IWD16_000874"
     CDS             8075..9856
                     /locus_tag="IWD16_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP9087956.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9849..11225
                     /locus_tag="IWD16_000875"
     CDS             9849..11225
                     /locus_tag="IWD16_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP9087957.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11232..11867
                     /locus_tag="IWD16_000876"
     CDS             11232..11867
                     /locus_tag="IWD16_000876"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP9087958.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11897..13252
                     /locus_tag="IWD16_000877"
     CDS             11897..13252
                     /locus_tag="IWD16_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP9087959.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13444..14838)
                     /locus_tag="IWD16_000878"
     CDS             complement(13444..14838)
                     /locus_tag="IWD16_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9087960.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14930..15901
                     /locus_tag="IWD16_000879"
     CDS             14930..15901
                     /locus_tag="IWD16_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9087961.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15898..16680
                     /locus_tag="IWD16_000880"
     CDS             15898..16680
                     /locus_tag="IWD16_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP9087962.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16849..17220)
                     /locus_tag="IWD16_000881"
     CDS             complement(16849..17220)
                     /locus_tag="IWD16_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9087963.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17354..17797
                     /locus_tag="IWD16_000882"
     CDS             17354..17797
                     /locus_tag="IWD16_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9087964.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17840..18649)
                     /gene="yidA"
                     /locus_tag="IWD16_000883"
     CDS             complement(17840..18649)
                     /gene="yidA"
                     /locus_tag="IWD16_000883"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP9087965.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18668..20035)
                     /locus_tag="IWD16_000884"
     CDS             complement(18668..20035)
                     /locus_tag="IWD16_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9087966.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(20019..20849)
                     /locus_tag="IWD16_000885"
     CDS             complement(20019..20849)
                     /locus_tag="IWD16_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP9087967.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            21012..21449
                     /locus_tag="IWD16_000886"
     CDS             21012..21449
                     /locus_tag="IWD16_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP9087968.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21525..22145
                     /locus_tag="IWD16_000887"
     CDS             21525..22145
                     /locus_tag="IWD16_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP9087969.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT         7128 a         3851 c         4786 g         6449 t
ORIGIN      
        1 tatattggat atatatattc attgaaaata gagcataaca actttataat gttgagtata
       61 ccaatttttg gaggcgtttt aatggaccgc tatttagatt tactttcaga gaagttccct
      121 acaacggaag atgtcatcac ggaaatcatc aatctcgaag caattttgca gcttccaaaa
      181 ggaacagagt tatttttgag cgatatacat ggcgaatttc ctgcttttga tcatattctg
      241 cgtatcggtt caggaaatct taaagaaaag atcaaagacc ttttctctga acaaatgacg
      301 gaaacggaga tcagccaatt cactattttt acggcttatc cagaatatgt gttgacgact
      361 gattggtaca aaaaacaaga taaatcccag ctgatccatc gtttgatcga tcttttacgg
      421 tttacgacag tcaaatacac tcgatcaaaa gttcgaaaag gattgccgaa agaatatagc
      481 tatatcattg aggaactctt gtatatcgat gatcgtatca acgggaaaaa agactatgta
      541 aaaaaaatca ttgaacaact tgtcttgatg aaggaagaag aaaaattttt aacaaaactt
      601 gcgcagacga tccaaaaatc tgtcatcgat catttgcata tcgtgggtga tatttttgat
      661 cgaggaactc aagcagccaa agtaatggat cgaatcatgg atttttcctc tgtagatatc
      721 cagtggggga atcatgatat tttgtggatt ggtgcgtatt gcggctctga ggcatgttta
      781 ttgacgcttt tacggattgc cgcacgctac aactatttat ttgaattaga gaaagaatat
      841 ggattaaatt tacgtccatt attttctttt gcccatcaaa cctatcagaa gaatccagta
      901 tttaccccta aaaacaggaa agatctatct gaaagagagc aagaagaatt agaaaagatc
      961 catcaagcac tttctatcct ccaattcaaa tcagaacatc agttgttgaa tcgacgacct
     1021 gaatttaaaa tggacgatcg taaattgttg gaaaaaatca actatgagga atcaacgatc
     1081 gttttagaag gtcagctcta tgagttgaaa gggacttgtt ttcaaacgat ccagccagat
     1141 gatcctaaaa aattcttgga agaggaacaa aaagtgatcg atagtttgat gtattcattc
     1201 cagcactcgt taagaatgca aaaacacatg acgtttttga tcgaaaaagg gggaatgtat
     1261 ctcacgaaca atcagcatgt actgttccat ggctgtatac ctgtagatga aaaggggcag
     1321 ctcttagcat ttgaattaga ccaatcttat cgcggaaaag aactgctggg attttttgaa
     1381 aaacagataa ctgaaagcgc aaaagatttg actgcaaatg aagatttggc gaccgatttg
     1441 atttggtatg cgtggagcgg gccattttcg cctttattcg gcaagagtaa aatggcaact
     1501 tttgagcgct attttctcac agagcctcat acgcacgtcg aaaaaagcaa tccctattat
     1561 cacttgcgag atgaagagtg gttcagtgaa aaaattttgg cagaattcga tgcaaaagaa
     1621 gaaggttcag caatcatcaa tggacacacg ccagtaaaag taaaaaaagg agagtcaccg
     1681 atcaaagcaa acggaaaagc atttgttatt gacggaggtc tatccaaagc ttatcaaaaa
     1741 acaaccggga ttgctggcta ctcattacta tgcaattctt acggtttcca aatcgtgaca
     1801 cattatccgt tcttgcctat tgaacaacaa tttgcaaaaa aatctgatca aacgaatgta
     1861 aaaaaagtga tcgagcagca gctcccacgc aaacttaatc ggaatacgac aaaaggaatg
     1921 gaattacagc aacagattac tcaactccaa aggctgttgg attatcgaaa ggacgattga
     1981 gaaaaatgct agccaacatt gctcaataga ctaaaaagcg actgcaacaa aaggcgtgtc
     2041 gcagtctcct tcggaattag ctcaaaaaac ggtaaaatat tagaagctat ttttctgttt
     2101 atttctctaa tactcaggtc agggcgctga tttcacgacc tcttccttaa acggtgttct
     2161 cctgccaact cctcggtcac aagtcacaat atccaacaaa catgagaagc tgttttttga
     2221 aattgctcct tgtgactcag agttgaacgg caggctcacg acctaatcag aaaaacgatt
     2281 aaaattcatt cacaaagaaa tcgaataaac gctgttcaga tgaatctaca cgatgagaaa
     2341 gttccggatg ccattggaca ccaaggattt tgtgcatatt atctttactt tggactgctt
     2401 caatgagtcc atcgttggaa aaggcgatgg cttttaacaa aggagaaaga tctttgactg
     2461 cctgatgatg ataggaattc acaaagtatg aatcagggag aaggtcactt aataaactgt
     2521 ccgaaacgat tttgacttcg tgagttgcaa actgcggggc agtgggctgt tgttcgtgtt
     2581 ttacggacca ttttggatat aatgaaagat cttgataaag tgttccctcc aacacgacat
     2641 tcaatagttg aagtcctcgg cagactgcaa aaatcggttt tctttgtttc aatgcttcca
     2701 agatcaatgc ctgttcgaat tggtctcgtt gaatatttgt ttccgtcagt tttggatgag
     2761 ggtcttccat gtaaagttgc ggactaacat cctgaccgcc tgcaagaatg agtttgtcga
     2821 ttttctcaat tagttgaggc gctgaatctg gagacatgac tggcagaata agaggtaatc
     2881 cttgagcatc taaaactgca tccacaaatc cctgcggtgt atagcttact tgatttcctt
     2941 ggaaagtatc tgtcgctcga atcagttgat tcccggcaat gccgatgatt ggtttcattt
     3001 ttattccccc taaagtagac taattgtaac aaggatttaa aaaaatttaa actatgtaag
     3061 acttgcccct atacttatct gttcttgctg tgttactatc tgtaaagaga attttcgtgt
     3121 gaaattcatg acgatagttg aaaatattgc ttaatttgtt ataataaaaa gtaagcagaa
     3181 ataaaaaggt tgggcgtaag cttcaaggat ctagttcttc aaggggtgag aaggacattt
     3241 tattgaaggg gcctgcctat actaagttat ctttagaggt acatactttc aatgaaaaaa
     3301 gaaagcatgt acctcttttt taaatcgaaa ggaagtgaag caagtgttag aagctttgga
     3361 acaaatccga aaagcagaag aagaaaatga gaagcgtgct cgaaccctac ggcagcagtt
     3421 gaagacctat gcagaagaaa aagagcaaga attggagata atccaaacga acagtcggca
     3481 acagctgaaa cagttggtag aagaaaaaga acaattcgaa aagcaagaat tagaaaaaga
     3541 aaaaatgaaa ttgaaagcag aagcagacaa ggtgcaagca actttgaaag accaatatca
     3601 aaagcatcag agtcaagcaa tcggtgcaat cattgaaagg gtgaaagaaa cttatggccg
     3661 tcactaagat ggaaaaagtg accctcattt cagataaaag aaatcaggag aggattttac
     3721 aagcagtaca aggaatccaa aatgttgaaa tccgggatct gtatcaaaat agaacaaaca
     3781 accagtgggt agaaaaatat tttccagata ccacgattat tgataaagaa gcaaaattaa
     3841 gcgctttagg caatcggctg aaagacatcc aagaagctat ccaattcata caacaccacg
     3901 gcgatagccg tcaaaagaaa ctccatttga aacgtcaaga actttcctta caagacttgg
     3961 aagcaaatta ttccgaagaa aatttcatga ataaattaga agaagtcctt gatttaaaag
     4021 aaaagtggga acagctaact gaaaaacgtg atcgtttaga agaagaggaa gaatggcttc
     4081 ttcattggca acagctggat attgctccaa gtatgtatgc aagtaagacg accatttttg
     4141 agatggggac tgtgagtgca gcaaattttg aaagtttcaa ggaagaacta agtaagcttg
     4201 atgacgttta tatggaagaa atagattata cgccaaaaca agtttatctt tcgtatgttg
     4261 ctttaaaaaa atcagttgct gctatagaag aaattgctgg gaaatacggg ttcaataaag
     4321 aaacgtatcc ttatgaacaa aatcctaaag aaaaattgca agaaaccaaa cagcaattat
     4381 ccattgttca cgagcagcaa aaaaagataa cgacagcgtt aggtcagtgc agcggctata
     4441 tccaagaatt tgaatgggta gaagaagtca cacttgccgt tgcagaacgg gaaaaagtaa
     4501 aagaacggtt tatccatgcc acttatctga tcgttttgca aggttgggta gatgcagaag
     4561 aaaaacagaa tttgatgcat gtgttaaacg aaactgtatc agaagaagat atatacgtta
     4621 gtttcgaaac accaaactca gaagagattc agcaggaagt ccctactaaa ttgaaaaacc
     4681 atccgcttat cgaacctttt gaattgctga ctgaaatgta cagtctgcca aaatatgaag
     4741 aagtagatcc aacgccttgg atgacaccat tttatcttgt cttcttcggg atgatggtcg
     4801 cagatgttgg ttacggattg ctgatgctca ttgggacaat actagctcaa aaactatttg
     4861 ttttaccccg cgggatgaaa cgtttcgtaa aattctttga gattctctcg gtaccatcga
     4921 tcatctgggg attgatctac agttcatttt tcggacaggc attaccaaaa gaactatttg
     4981 gtatccgctt gcctttccca atcctatcta cgacggatga cgtcaatacg attttgattt
     5041 tatccgtgat tttcggtctg atacagatct tggttggatt attcgtggca gcaaaggaaa
     5101 atatcaggag gaaagattac ttgagtgctg tcagtgatgg ttttgcctgg caagggatcc
     5161 tgattgggat cgttattgca ttgattggtg tcatgttgat aaaaaataca gcatttgttt
     5221 atcttggcgc ggggctatcg atcattgctg ctttatgtat cttaatcgtg ccgattatcc
     5281 aatcgccgtc taaagtcaag ggtgctgcca aaggagcgta taatctttac ggattgactg
     5341 gttatatcgg tgatttagtc agttatacac gtttgatggc actaggtatt tcaggcggaa
     5401 gtattggcgc ggcattcaat atgctagttg ctttcatgcc gccagccgct cgattcagtg
     5461 tagggatcct tttgatcatc gctttacacg cactgaatat gtttttgaca ctgttaagtg
     5521 cctatgtcca tggtgcacga ttgcagtatg tcgaattttt tggaaaattt tatacaggag
     5581 gcggacgtgc ctttgacccg ctgaaaacgg cggaaaaata cgtcaacatc aatcataaga
     5641 aaaaaaacaa taaataattg gaggattttt atacaatgat ggattactta attagtcaaa
     5701 atggtggaat ggtatttgca gtattagcga tggcaacagc gacgattttt tcagggattg
     5761 gttcagctaa aggtgttgga atgacaggtg aggcagcagc agcgttaaca acaagtcaac
     5821 ccgaaaaatt cggacaggct ttgattctgc aattgcttcc aggtacgcaa gggttgtatg
     5881 gattcgttat cgcctttttg atttttatca acttaggaaa tgatatgtca gttgtacaag
     5941 gattgaactt tctaggcgct tctctgccga ttgcttttac aggtttgttt tcaggtatcg
     6001 cccaagggaa agttgcagca gcaggtatcc aaattttagc gaaaaaacct gaacatgcta
     6061 caaaaggaat catttttgct gcgatggttg aaacatatgc gattttaggt ttcgttattt
     6121 ccttcctatt agtattgaat gcctaagcat aaaggaggaa aagcagatga acgccattga
     6181 aaaaatcatc tctcagatga atgaagcagc agagcaagaa agagcggcgt tagagcaaga
     6241 agagcgaatg aagatcgatc aaaactttga acaaaaacgt acgcaaatcg aaacagaaca
     6301 tcaaaaacaa aaagaaaaac aaatcgaatt acttgaaaaa aaataccgtc agcttaggaa
     6361 tcgccaacaa gtagaagtac gacaagaaaa tttgaatgcg aagcaggaat ttttacgacg
     6421 attgtttgca gatgcaatag ctgaaatgga gaattgggat gaatctgaac aaatccagtt
     6481 tatcaaaaat tcattgtatt cattaccttt gacaggcaaa gtaacattca ttgctggaga
     6541 aaaatcagca gcttatcttt ctcagacatt gctggatgaa tggaacaatg aacttccctt
     6601 tatgatggtt ttaagtgatg aaactgttgc tgatcaagcg ggttttctga taaacgatca
     6661 aggagttcag tataattttc ttttcagcag ccttgtacaa gatatccaag gaacgatgag
     6721 ttttgagatt gccaatcaat tattcgaata aggagggatc agtgtatgga ataccacgaa
     6781 ctaaatccct tgattcgcgg aagagagctt gaacttttgt ccaatgagac atttgaacgg
     6841 atgattcaaa caaattctgt cgaagcactt ggtgaaatat tgaaatccac gatttacagt
     6901 ccatatattt atgacggata tgaatatgac ttcgaacaga atcttttaaa ggagcagagc
     6961 cgtctatttc gctggttaaa ggaatcagca ccggagcctg aaatcgtttg gatctatacg
     7021 atgcgctata ctttccataa tctgaaggtt ttgacaaaag ccgagatgac aaatcaaaat
     7081 ctggaccatt tgtatatcga cgatggattt tattcattag aaaccttgaa ggatgccatt
     7141 catacgcaag tttcaaatga actccctgaa agcattatgg acagtatacg agaagtacgt
     7201 gattattttg aagagtctac cattcttcag ggaattgacg tgatttatga tcgacagttt
     7261 ttgacagagc agcgacggct tgccgataag ctaggatatc aagaattgct gacagaagtc
     7321 attgctttta ttgacttgac caatatcaca acgatggctc gaggaatatt gcagaaaaga
     7381 acatatgggt tcatgacgac ggtactttcc agctcgggaa gtatcgaaaa agaagtattt
     7441 ttgaactttg tagataaaga tatggaagat tacacgaatt ttctattaga gacagattat
     7501 gctgattttc tgcgccctgt catccgtgag ggaaatcttg atctcgtaaa gcttgaacag
     7561 ttgaaagatg attatctaag ttcattgtat caagaagcac aaacccaggc tttcggacca
     7621 ctgccactac tagccttttt gaacgcaaaa gaggtagaaa gcaaaaacct tcgtttactg
     7681 gttatcggca aaagaagcca tttcgaacca gaaacaatca gaaagcgggt gagacaagtc
     7741 tatgacgcat aagatcggtg tagttggcga caaagattct gttttgccat tcaaattgtt
     7801 tggctttgat gttcgttatg gtacaaccaa gcaaaccgtt cgtcaggcaa ttgaagaaat
     7861 ggcaaagaat gagtatggcg tgatttatgt cacagagcaa tgtgcagcat tgagcccaga
     7921 aacgatcgaa cgttacaagg aacaaatgat acctgctatc gtattgattc ccaatcatca
     7981 aggatcatta gggatagggc tggaagaaat tcaaaaaaac gtagaaaaag cagtaggtca
     8041 aaatatctta tagtatgaaa aggagataga aattttgcaa attggaaaaa tcataaaagt
     8101 ctccggacct cttgtaatgg ctgagaacat gtctgatgcc agtatccaag acatgtgtct
     8161 agtgggagat ctaggagtta tcggagaaat tatcgagatg cgtggcgatg tggcgtctat
     8221 tcaagtatat gaagagactt ctggaattgg tccaggcgaa ccggttcgct ctactggtga
     8281 agctttatct gttgaattag gaccagggat catttcacaa atgtttgacg gaatccaacg
     8341 gccattagat acctttatgg aaatcacaca aagtaatttc ttagggcgcg gagttcaatt
     8401 gcccgcattg gaccatgaga aaaaatggtg gttcgaacca acagtagaag ccggagaaac
     8461 ggtgagtgct ggagatgtta tcgggatcgt agaagaaaca aaagttataa aacataaaat
     8521 catggtgcca aatgggataa aaggaacgat caaaacaatc gaaaaaggtt cattcactat
     8581 tgatgaaaca gtatgtgtca ttgaaaccga aaatggtgac aaaaatttga cgatgcttca
     8641 aaaatggcca gtaagacgta gtcggccaat caaagaaaaa ttaaacccgg atgcacccat
     8701 gatcactgga cagcgagtga ttgatacatt cttcccagtg acaaaaggag gagcagcagc
     8761 tgtacctggt ccttttggtg cagggaaaac agtcgttcag catcaaattg caaaatgggc
     8821 cgatgtggac atggttgtct atgtgggatg tggcgagcgt ggaaatgaga tgacggatgt
     8881 tttaaatgaa ttccctgaat tgattgaccc taatacagga gaatcgttga tggaacgtac
     8941 ggtattgatt gctaatacat caaacatgcc agtagcagct cgagaagctt ctatctatac
     9001 gggaatcaca attgcggaat atttccgtga catgggttat gacgttgcta tcatggcaga
     9061 ctccacttca cgatgggcag aagcacttcg tgagatgagc ggacgtttgg aagaaatgcc
     9121 tggtgatgaa ggttatccag cttatcttgg ttcacggctt gcggaatact atgaacgatc
     9181 tggaagagtc attgcattag gatcagaagg tcgcgaagga agtataaccg caatcagtgc
     9241 tgtatccccg tctggagggg atatttcgga accagtcaca caaaatacat tgcgggtcgt
     9301 aaaagtattc tggggtcttg attccagttt agcccaaaaa agacattttc cttcgatcga
     9361 ttggatacaa agctattctc tttattcaac agaagtaggg aagtatatgg atcatatcct
     9421 tcaagctgac tgggcatcca tggtcaccga gggcatgcgt attttgcagg aagaacagca
     9481 gctgaatgaa atcgtgcgtt tggtagggat tgattccctt tcagataatg accgtctgac
     9541 attagaagtg gcaaaatcca ttcgtgaaga ttatttgcag caaaatgctt ttgatgatgt
     9601 cgatacattc acttcaagag agaaacaatt caaaatgttg aagatgatct tgacctttgg
     9661 tgaagaagga cgccgtgcgt tgtcgttagg tgcttattta agtgagatca tggacggtac
     9721 cgtatcgatt cgtgaaagga tcggacgaag caaatatatc ccggaaacag aattagaaaa
     9781 actagatcaa attattggtg aaatcaagca aacgatgcaa acgatcgttt cagaaggagg
     9841 catgagcaat gattaaagaa tatcgtacga tcggtgaagt cgtcggtcca ttgatggcgg
     9901 ttgaaaaagt tgctggtgtc aaatatgaag aattaatcga agtccgtatg caaaatggcg
     9961 agattcgtcg tggacaagta ttggaagtcc aagaagataa agcaatggtc caaatattcg
    10021 aaggaacaag tggtatcaat ttacgtgact cttctgtacg atttttaggc catcctttag
    10081 aactaggtgt atcggaagac atgatcggtc gagtattcga tggtttggga cgaccgaaag
    10141 ataacggacc agagatcctg cctgaaaagc atttggatat taacggagaa gtaatcaatc
    10201 cagtagctcg ggattatcca gatgagttta tccaaacagg tatctctgca atcgatcatt
    10261 tgaatacatt agttcgtggg caaaaattac cagtattttc tggctctggt cttcctcata
    10321 aagaattagc tgctcagatc gcgcgacaag caacggtact cgattcatca gatgatttcg
    10381 ccgtagtctt tgctgctatt ggaatcactt ttgaagaagc tgaatttttc atggaagatt
    10441 ttcgtcaaac gggcgcaatc gaccgttctg tcatgtttat gaatttagca aacgaccctg
    10501 caatcgaacg tatcgctact cctcgtatgg ctttgactgc tgcagaatat cttgcttatg
    10561 aaaagggaat gcatgttttg gttatcatga cagacatgac caactatgca gaggcattgc
    10621 gagagatttc tgcagcacgg cgagaagttc caggaagaag aggatatcct gggtatcttt
    10681 acacgaactt agctacttta ttcgaacgag caggaagaat ccgcggacta aaaggatcgg
    10741 ttactcaaat tccgattcta acaatgccag aagacgataa aactcatcca atccctgact
    10801 tgactggtta tattacagaa ggacagatca tcttgactag agagctttac aagagtggga
    10861 ttcagccacc aattgatgta ctcccgtctc tttcgcggtt aaaagataag ggaactggac
    10921 ccggaaagac aagagaagac catgcagcta cgatgaatca gctttttgca gcctatgcac
    10981 aaggaaaaca ggcaaaagaa ttagccgtag ttttaggtga atctgcttta tctgatgtag
    11041 acaagatcta tgcgaagttt gctgagcggt ttgaaaaaga atatgtcaat caaggattct
    11101 acacgaaccg ttcgattact gaaacacttg atctagggtg ggaactgcta tcgatgttgc
    11161 caagaaccga attaaaacga atcaaagacg acatgctgga caaatatctg ccagaaggga
    11221 agtgagttct gatggcacga ttgaatgtga atccgacccg catggaattg actcgattga
    11281 aaaaacagtt gacaactgcc acaagagggc ataaattatt aaaagataag caagatgaac
    11341 tgatgcgtca attcatcctg ttgattcgaa agaacaacca gctgcgtcaa gaaatggaag
    11401 agtctatgac agaagcaatg tctgattttg tattggcaaa tgcttcgatc acggaacctt
    11461 tcatcgaaga attatttgtt ttgccggcgg aaaacgttga tttgtctatc gtagacaaaa
    11521 atatcatgag tgtaaaagta cctgtgatga attttgaata tgatgaacag ctgacagaca
    11581 cacctttgga atacgggtat ttgaattcca gtgccgaact ggatcggtca atcgatcggt
    11641 ttactaccct gcttccaaaa cttttggaac tgacagaaat cgaaaaaact tgtcaactga
    11701 tggcgagtga aatcgaaaag acacgtcggc gagtcaatgc attagaatat atgacgatcc
    11761 ctcaattaga agaaacgatc tattatattc gtatgaagct ggaagaaaac gagcgcgcag
    11821 aagtaacaag gttgatcaaa gtaaaaaata tgggtaccac tgactaagtg gtatcttttt
    11881 ttaaaagagg aaaacgatga ttaaacaaag caaacaaata aagaaacgtt tgccggctgt
    11941 tcagcttctc gcattaggat tttttctcct gattttagtt ggcggcggcc tacttaccct
    12001 accatttttt agtaatagtg ggcatgggac aaattttatc gatgcgcttt tcactgcaac
    12061 ttctgctgtc tgtgtaacgg gattgacaac attgaataca gcagagcatt ggaacagtgc
    12121 tggtcaattt ttgatcatgc ttttgattga gatcggcggt cttggtttta tgatggttcc
    12181 gattctatgc tttgcattga taaagaagaa agtcagtctc agcacacgta ttatattaaa
    12241 agaagcgctg aatctagaag aagtatcagg tgtgatgaag ctcatgcttt acatcctaaa
    12301 attggccttt gttatccaat tgattggtac ggttgcttta tcttttgtat ttgtgcctga
    12361 atttggctgg ttgaaaggta tctggtacag tttgttccat gcaatctcta gtttttgtaa
    12421 tgcagggttt gatttgttag gagacagttt agccaatcac cagcaaaatg tttatttgat
    12481 catggtggta tctgctttga tcatttctgg tgggttaggc ttcatcgtat ggcgtgattt
    12541 actggaatat catcagaaaa agaagatgac gattcattca aaaatcgcat taaccgttac
    12601 aggtaccctt ttattaggcg gattcgttgt gttctttttc acagagcgca atggggcaac
    12661 gctagtggaa gggaatatgt ttcaacgatt ggcaaacacc ttttttatga gtgtgacacc
    12721 tagaacggct ggatattatt cagttgatta cctccatatg agccatgcag gtctgatttt
    12781 aacgatgttt ttgatgtaca tcggcggaac atctgggtcg acggccggcg gattgaaaac
    12841 aactacgtta ggtgtgttgc tgatccagat gcgttcgatg ttgaaaggac gtacgagagc
    12901 ggaagcattt ggccgtacca tccgacaagg cgcagtgttg cgggcactta ctttattttt
    12961 tgttacgttg acgttgtgtg tagtcgccat catgatttta tcggtgactg aaacgatacc
    13021 taaaacttct ggcatcgaat atattgcttt tgaggtcttc tctgcgtttg gtacagtagg
    13081 tcttacgatg ggattgacgc cagacttgac tgcaataggg aaattgatca ttatagcgct
    13141 tatgtacatt ggacgagttg gtgtattgac tgtggtcttc tctctgatga caaaagcaca
    13201 aagaacacaa tcaaaataca aatatccaga agaaagtgtc atgattggat aatttcatct
    13261 tctgacagaa acctagtgat ttagtaaaat aagaaaataa aataattttt cttgcttctt
    13321 ggatacttta agtgcaaaat gttcgtttat tttttgaggc actacccgtt gttgaacctc
    13381 gggtagtgcc tcttttttat tgaaaggaat caataagatt tgattccttt atttatcaat
    13441 aggttataat tcatattttt ttattttttc ttttacgata cttagtccag aagctaaaat
    13501 ttcaaggtta ttagaggata gggaaattcg aacgaatgca tctggttgcg cacgtgtagt
    13561 aaagcgtctg gaatgataaa gtcggatacc gttagcaaga aagtcattct caattatctt
    13621 agggtcaatc tcaggatcaa ctggaagaat tcgatagtaa gggacacgat tcgttggttt
    13681 ttctactttg aaaagagaat caaaaagctg attggctctt tttgtaagac ttaatttagt
    13741 ttctaagatc ctatttgctt catttgaatg gaggatttga gtgatgactt ccacatctaa
    13801 tccagaagtt ttcacattta tattaaaaat tgcttgttct atcttgcttt ggacatgtac
    13861 tggaactaca agataagcaa ctcgcaatcc agaacaaata aattttgtca ttccagctaa
    13921 gtagattgtt tggttaggta ataaattttg gaaaggagga atgatcgtat gttgatagta
    13981 agttgttaaa aatgaatgga tatcatcttc aataacccat agaccttctt tttcaatcac
    14041 ttcggctaat tgctctcgtc ttttgtgaga gatttgaaac cctataggat tattacaaga
    14101 aggcataaga aagacaccat gaatcttctt ttttctacat tcctgaatca atatatcagg
    14161 tcgcattcct tgctcgtcaa aatcaattgg aacgatctct aaatgatata actgagccaa
    14221 ttcaatgaaa ttagcgtatg tatatcggtc tgttgctatt ctatcacctg gagagaaaag
    14281 tgcagaaagt gtgatagcca atccattttg aacaccggaa acaatagata gagattctaa
    14341 ggacggaatg actccttgag tttttaacca atttgctgca gtagtcaatt gaaaatttgt
    14401 gcctttggga tcttggtaat taagcaaacc aacaagattt gggttattac tagttgattg
    14461 aataaacggg acaacgaatt tattacattc ttcaaaagaa ctaaccagac caaaatcaat
    14521 gatatgttcg gttacttcat tggtagagat ggtgatggac tcaaagacat tgggagaaat
    14581 aaatgtgcca cttccaatat tcgtatacag caaacctttc tcaattccat atttataagc
    14641 cttgccaacg gtagtaaaat ttaaatctaa ataatcagct agctctcttt gggagggtaa
    14701 ctgagtgttt ttctgtaatt tattctttaa aatatcttgt tctaattgtt gaatcaacga
    14761 tcgataataa ggacgcttca atttttgttt gtctggtcgc caagttaacg ggtaattgtc
    14821 ataagaatta ataggcattt tctccactcc ttaaaattgt ataccataca attttaccat
    14881 tgattgtatt gattttagaa gttaatcttt tgtgaggagg gaattgtcta tgaaaattat
    14941 taattttgag gaagtagtag cttcttttag ttttgaagaa gcgattcaag taatgaaaaa
    15001 atgttttttt gattatgaaa gtggtaaaat cagccaaaat cctcgattgg tggaaatttt
    15061 acctgacgga aaaggcaaaa atatgtttgc gatgatgcct gcttatttgg gggagaacag
    15121 atattttggc acaaaagtca ttaccgcttt tccggataat catcagtatc atttaccttc
    15181 tcatttagga gagattttct tgtttgatgc tcaaaacgga ctacccaaag cattgataaa
    15241 cgcaaatgca gtcacctgga ttcgaacagc tgctgtatct gcgctggcaa cagattactt
    15301 agcaaatccg catgcagaaa cacttgcttt aattggagct ggtcaacaag cattgtcgca
    15361 tttgactgcg atttctacca ttcgtcccat cactacagtg tttgtttacg atattttaga
    15421 agaaagacga aatactttta tcgctaaagc taaaaaacag tatcctaaaa taacgtttat
    15481 aaatgcaata tcagtagaag atgcagtacg tccagcggaa atcatttgta cgttgacctc
    15541 tagtaaagag gcatttcttc aaaaagagtg ggtaagggaa gacgcacata tcaatgctgt
    15601 gggcacattt actcctgata caagagagat aagtacatcg ttgatggaaa cttctcaaat
    15661 ctatgtagat gattactcag ctgccttaac agaaagcggg gacctgctta tagccatggc
    15721 agaaggagat ttagaggaat cttgtattgt tggtacatta ggcgaattag tttctgagaa
    15781 aaaaacattt tataaaggag aatcgaggcc gacgattttc gacgcagttg gtctagctat
    15841 tgaagatctt tgttgtgcag aatatattta taacaaatta gagaaagggg ctttaaaatg
    15901 aacttagatt attacgtagt tgatgcattt gcagatgagg tgttcaaagg aaatccagca
    15961 gcggtttatg tgctagaaga atggctgcca gaaggtacga tgcaaaaaat tgccattgaa
    16021 aacaatttat ctgaaacagc atttacagtg aagaaaaacc aagaatttga gcttagatgg
    16081 tttacgccag atcgagaaat cgatctttgt ggtcacgcga cattagcaac agcatttgtt
    16141 ttatttaact attacaaaat acctgatgaa acgattaaat tttctactca aagcggtaat
    16201 ctatttgtga caagaattaa agattattat tacatggatt ttcctagtat tatgccgaaa
    16261 aaagtgccga tattgtctga atatgaagaa gcgattgggg ctaagattaa agaagcctac
    16321 ttagcacgtg atttattttt cgtattagaa gatgaaaaaa cggtggctaa attgcagcca
    16381 gattttacag ctatcaaaaa ttttgaatta ggaataggag tcatcgtgac agcagaaagt
    16441 gtgcaaaaag actttgtttc tagaaccttc tttccaaaat taacaatcaa tgaagatcca
    16501 gtatgtggat cagcacattc taatcttatt ccttattggg cagaaagaat gaataaaaca
    16561 aaattgtcag cttatcaaat gtcatcaagg ggcggggatc tagaatgtga attaaaagaa
    16621 gatcgtgtga taataggagg aaaagcagtt ttgttttcta aaggtacctc gtttatttga
    16681 tttctttcaa atgaattaaa aataaattgg tggagagtgg caaccttata gctacaatgg
    16741 ttgttaaaaa aagacgcaaa ctttttcaat ttaatgaaaa gtttgcgtct cattttaatt
    16801 attggtgttt gttttttatt caatcaagag taaaatgccc catactaact atcctcggat
    16861 gacttcgatt ttataaccat cagggtcaat gacaaagtag taagaaggcg cagtgccagg
    16921 taatcctttt aagtcagtaa tctcaagacc ggcagcttta tgttgctcgt gtaatttttc
    16981 tagatcatca gctgctatag cgatatgacc atagccgtct cctagatcat acgcaccatg
    17041 atcatagttg taagtcaact ctaattcata gtcatcccca ggaagtgtca aataaacaag
    17101 tgtaaatttg ttttctggaa aatcacggcg gcggctttct tcaaaaccaa atgccttttg
    17161 ataaaattcg attgatgctt caagatcttt cactcgaaca catgtgtgag ccattttcat
    17221 agattcatct ctccttttcc ttttactcaa tcataacaaa aagaagtaag gaaatagttg
    17281 atttgtgctt gttcatgaga aatttctcat gtaaaatgga tagaaaataa aactgttaag
    17341 gtggaaacta ttcatggata aaccaatttt tggtgaaaaa aagcaatttg ctgattatca
    17401 aacccgttat gcagcatata ttgtcataga aagagatcag cagattgcag taatcgaggc
    17461 tccaaatggt gctttttttc ttcctggcgg ggaaattgaa ggagaagaga cgaaagaaga
    17521 agcgattgtc cgtgagctga tagaagaaat gggaattgtg gcagaaattt cttcttatct
    17581 gggagaagct gatgagtatt tttactcgaa ttatcgtgca acttattatt ataatccagg
    17641 atatttcttt gttgctgatt cttggaagcg tataggagaa ccaactgaaa agaccaataa
    17701 aatttggtgg gtgagccctc aagaagcgct tgtgaaattg aaacgtggta gccaccgatg
    17761 ggcagtcgag agatggatcg aaaaaaataa aaactgaaag aaagcgagta gatcagcttt
    17821 ctctcagttt ttttatttat tacagtacat attttttgat tgcttcagct acaccgtgct
    17881 cgtcgtttga tgaagtgatg acatttgctg cttctttgac aagagaaacg gcatttccca
    17941 ttgctactcc tagaccagca tattcaatca tggataagtc gttctcgtta tctccgatag
    18001 ccatgatttc atcttgatca ataccaaggt gctgtgcgag attcgcaact gccgcacctt
    18061 tacttgcttc tttatttagt acttcgaagt aatagggcgc acttttgacc gttgtgtatt
    18121 tatcacggaa agtttgcggt aaacgcgcaa ttgctgcatc taaaatttct ggttcgtcga
    18181 tcatcatcat tttgacgatg ttcatgtctg gtgtcatttc ttcaggtgtt cgatatttta
    18241 gcggcatctt gactaaactt gcttcgtgga tcgtataagc gctgatatct cgattaggcg
    18301 tgtagatcgc tttgtcatcg atcgaatgga gatgactgcc gacacggcgt gccatcactt
    18361 cgatctctag gaaatcttca tgtgtcagtc cgtgacgggc gatgatttta cctgatttcg
    18421 tttgttgtac gagtgccccg ttataagtaa taacgtaatc attatcttga aaaaggttca
    18481 actcgtttaa ctgttcctgg acacctggta agggacggcc agtgcataaa acgatcgaaa
    18541 caccttttgc aacagcagcg gcaatcgctt cttttacttc ttttgttact tctcttttct
    18601 catttaataa agtaccatcg atgtcgatag cgactagttt gatagacata atttcctcca
    18661 atatatttta atttatttca atcaatgcgc cattacggat atggctggca aattcttgat
    18721 aagtttcgtc aaataaatca taatgatcac gcaaggacgg atcaaccatt tcttttggaa
    18781 agaaaaatcg ctcatctcct tgttcttgtc ccgctaatgc agcaacaagc tgactaacct
    18841 gtgataattc tatcaaagtt ccgtcatttc gcaaaatctc gatttgtgtg cgatgtctgc
    18901 cttgttctgg gcgataaaaa tcataaggta gatcgtagct agaattcaca gcagtgtaat
    18961 aaacaggatt atatccgacc ttttctacta acaaagtgag ttcttggact aattttgaat
    19021 cccggttgcc agaaaaagta gcagatttca gtggtttacg attcaaaaaa cgtttagcta
    19081 aatcatttaa aataggatca gcagattcag accattgagt aaagtaagtg cccaagaccc
    19141 catcatcaag tttgaggtat tcttctaaag taaagcttcc gtcaagaaat ggcagaagta
    19201 attgagaatg gagttcaaaa ctgctggcat cttcttgata caactcgtgt gcgcgatgaa
    19261 gcagatgctc caaaattact tccataccac gggaaacagg atggaagtac acttgcacat
    19321 acatttgata tcggctaacg atataatctt ccactgcatg catgccactc atggaaaaag
    19381 cgatcccgcc ttcataagga cgaatcacac gtaatattcg ggtaagatca aatgttccat
    19441 attctgttcc agtgaaataa gcatcccgaa gcaaatagtc cattcgatct gcatcgatct
    19501 gactagaaat catttggaca acttgtgggt tggggtaagt tttttggatc acactagcta
    19561 ccttttcagg aaaaccttct tctactcggt taagaatctg atacacttct gtttctggag
    19621 aagtgatgat ctctacagtg atggcttcat gattcgtatg gaagatatgt tcaaatgtat
    19681 gagagtaagg accatgaccg acatcatgca acagtgccgc acataaagtc accagccgtt
    19741 cctgatcatc ccagcctccg ttgcctattt tttcttttga aaaatttcga gaaaagatat
    19801 cgcagattcg tcttgaaatc tcataaacgc ctaatgaatg agtaaaacgg ctatgttctg
    19861 ctccgtgaaa agtgaaagaa gaagtgccta attgtttaat gcgtcgtaga cgttggactt
    19921 ctttcgaatt gatcaaatct aagattactt gatgctggac atggatatag ttatggactg
    19981 gatcacgaaa aactttttcc ataggcagca tttgatattt ataaggggtt gtcataatgt
    20041 agctccttta tgttattacg ttgttccatt ctctggattt ggttgttcca ttgctccagc
    20101 tggatttctt tttttagcca gtcggtagaa tcctgtaaat cacttgcaga atacagcttt
    20161 tcaaaagctt ttgcaaatgc ttctttgact tcttttacaa aaaggggttg gtcaagcagt
    20221 tcttcaagtg ttgccattgt agcaggatcc actgcaggat agccatttgt accaaagtct
    20281 tcttttaaag cagattgata aaatcgcttc atgacttgcc cgcgtttttc ctgattaccg
    20341 ttgacactta agtacatcat aacagaaatc ccatttttga tccgacgctg agcgataccg
    20401 gcaaattttt tgccatggat gcttaaatca taagttccag gacaataaga atgggtgatt
    20461 tcgaatgctt gtatcggtgc ctttggaaaa gcgtatttag tcaataggag catttgctcg
    20521 taagcgtcat ctatggttat tttttttgtg tcactttggg ggaaaatcaa agaaatattc
    20581 aaaattcctt catctgatac gatccctaat ccacctgaat ttctgatcac tggagcgtaa
    20641 cctgcatctt tcagtgtttg gatcccgtca cttagaaaag gagttcgaac atccttcata
    20701 cctaagatga ctgtgcagtc atattgccag aaatgtaaaa agatttgctg gtgttcttta
    20761 gaatattccg ttaatatatc tgttaatgca aaaggggaaa agtcgttttt ttgataaatg
    20821 tgctgatcta gtcgaatcgc agggttcatt caaccggctc ctttgtataa attcatattt
    20881 tctacaatat tatactctaa aatcaaagag ttgttttgga aaaaaggttt tctgtaaaaa
    20941 tggacaagtt ggttgacaaa acaaaagaaa ctaggaacaa tgtagctatg agaagtaaag
    21001 gagaatgaat aatggaatta tcacaaggaa caccgatttc aatcaaattg cgtacaaaag
    21061 taaaacagca aggggaagtg caagattttt attttgattt gaaagggcaa atggtgacaa
    21121 ttggcgatac gctgtacata cgttataaag agattcagga ggagacgggc gatgagattc
    21181 ctgtaacgat caaattagtt ccagatggtc atgtacagct gatccgtgca ggagaaatgc
    21241 ggatgcgttt gaaatttggc tataaagaac ggttagaaac atcctatcga acaccatacg
    21301 gtatgatcca gattgctact tttacgaaag aactgcatgt tagtttgaaa gaccgtccca
    21361 caggagggaa ggttcgaatc gattatgatc tgttcatggg accggaaaaa atcggcgaat
    21421 attatttgac cttggatttt acagcgtaaa tataaaagaa aagtttgatt ttttgtgtct
    21481 gattttgtat gataaggagt agactgtgaa aggacgtgtc ctgattggaa attagtgtat
    21541 ttgaaggtgc aaacaaaagc gaattatcaa tgatcgaagt agcacacgcg atcttagaac
    21601 aacgtggcga tgtcatggat ttttctgatt tagctaacca gatccaaaac tatcttggga
    21661 aatcagacag tgaaattcgt gattcactcg cacaatttta tactgacttg aatattgacg
    21721 gcagctttat ctcattaggg gataatcgtt ggggcttgcg ttcatggtat gctatcgatt
    21781 cgatcgatga agaagtaaac catggaatgg acgaagagga tgaagatact ccacgtcgcc
    21841 gtaaacgcaa gaaagtcaac gcctttatca atgatgatga agacgcgatc gattacaatg
    21901 acgatgaccc agaagataca gatctgacag aagaagatga cgatgatctg tttgatgacg
    21961 atgatgatga agatgaagaa atcgctgctt ataattctga cttgcaagaa atcggagcag
    22021 atgacccgac agatgatgaa gaagacttgc caggaaatat cgaagaagat ttaagtatca
    22081 tagacgaaga cgatgatgat gaagattatg aagaggaaga attttctgac tctgaaaaag
    22141 aataaacatt attttcttac gagaggttgt gacagagacg atcagcctat cagaataagg
    22201 aggtgtgaca cgat
//
LOCUS       DACDMM010000030        21912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDMM010000030 DACDMM010000000
VERSION     DACDMM010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.030"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWD16_000888"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWD16_000888"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP9087970.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWD16_000889"
     CDS             complement(1172..1867)
                     /locus_tag="IWD16_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP9087971.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWD16_000890"
     CDS             complement(1880..2596)
                     /locus_tag="IWD16_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP9087972.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IWD16_000891"
     CDS             2926..3534
                     /locus_tag="IWD16_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP9087973.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IWD16_000892"
     CDS             3859..4182
                     /locus_tag="IWD16_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9087974.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IWD16_000893"
     CDS             4364..5734
                     /locus_tag="IWD16_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP9087975.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IWD16_000894"
     CDS             complement(5864..7165)
                     /locus_tag="IWD16_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9087976.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IWD16_000895"
     CDS             complement(7519..9510)
                     /locus_tag="IWD16_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP9087977.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IWD16_000896"
     CDS             complement(9654..9920)
                     /locus_tag="IWD16_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9087978.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWD16_000897"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWD16_000897"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP9087979.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWD16_000898"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWD16_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP9087980.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IWD16_000899"
     CDS             13264..13827
                     /locus_tag="IWD16_000899"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9087981.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="IWD16_000900"
     CDS             complement(14080..14601)
                     /locus_tag="IWD16_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP9087982.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="IWD16_000901"
     CDS             complement(14604..15446)
                     /locus_tag="IWD16_000901"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP9087983.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="IWD16_000902"
     CDS             complement(15439..16164)
                     /locus_tag="IWD16_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP9087984.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="IWD16_000903"
     CDS             16340..16636
                     /locus_tag="IWD16_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9087985.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="IWD16_000904"
     CDS             17204..18796
                     /locus_tag="IWD16_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9087986.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="IWD16_000905"
     CDS             18809..19549
                     /locus_tag="IWD16_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9087987.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="IWD16_000906"
     CDS             19717..21066
                     /locus_tag="IWD16_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9087988.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="IWD16_000907"
     CDS             complement(21194..21784)
                     /locus_tag="IWD16_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9087989.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DACDMM010000031        21515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDMM010000031 DACDMM010000000
VERSION     DACDMM010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.031"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            123..1079
                     /locus_tag="IWD16_000908"
     CDS             123..1079
                     /locus_tag="IWD16_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9087990.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1152..1268
                     /locus_tag="IWD16_000909"
     CDS             1152..1268
                     /locus_tag="IWD16_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP9087991.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1323..3005)
                     /gene="recN"
                     /locus_tag="IWD16_000910"
     CDS             complement(1323..3005)
                     /gene="recN"
                     /locus_tag="IWD16_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP9087992.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(3023..3472)
                     /locus_tag="IWD16_000911"
     CDS             complement(3023..3472)
                     /locus_tag="IWD16_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9087993.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(3579..4397)
                     /locus_tag="IWD16_000912"
     CDS             complement(3579..4397)
                     /locus_tag="IWD16_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP9087994.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4406..5296)
                     /locus_tag="IWD16_000913"
     CDS             complement(4406..5296)
                     /locus_tag="IWD16_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9087995.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5298..5522)
                     /locus_tag="IWD16_000914"
     CDS             complement(5298..5522)
                     /locus_tag="IWD16_000914"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP9087996.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(5527..6864)
                     /locus_tag="IWD16_000915"
     CDS             complement(5527..6864)
                     /locus_tag="IWD16_000915"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP9087997.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(6865..7719)
                     /locus_tag="IWD16_000916"
     CDS             complement(6865..7719)
                     /locus_tag="IWD16_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP9087998.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(7816..8265)
                     /gene="nusB"
                     /locus_tag="IWD16_000917"
     CDS             complement(7816..8265)
                     /gene="nusB"
                     /locus_tag="IWD16_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP9087999.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8258..8683)
                     /locus_tag="IWD16_000918"
     CDS             complement(8258..8683)
                     /locus_tag="IWD16_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9088000.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(8929..9993)
                     /locus_tag="IWD16_000919"
     CDS             complement(8929..9993)
                     /locus_tag="IWD16_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9088001.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10165..10410)
                     /locus_tag="IWD16_000920"
     CDS             complement(10165..10410)
                     /locus_tag="IWD16_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9088002.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(10549..10923)
                     /locus_tag="IWD16_000921"
     CDS             complement(10549..10923)
                     /locus_tag="IWD16_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP9088003.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(11006..11920)
                     /locus_tag="IWD16_000922"
     CDS             complement(11006..11920)
                     /locus_tag="IWD16_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP9088004.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(11941..12744)
                     /locus_tag="IWD16_000923"
     CDS             complement(11941..12744)
                     /locus_tag="IWD16_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP9088005.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(12777..13766)
                     /gene="manX"
                     /locus_tag="IWD16_000924"
     CDS             complement(12777..13766)
                     /gene="manX"
                     /locus_tag="IWD16_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP9088006.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(13869..14360)
                     /locus_tag="IWD16_000925"
     CDS             complement(13869..14360)
                     /locus_tag="IWD16_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP9088007.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(14551..17385)
                     /locus_tag="IWD16_000926"
     CDS             complement(14551..17385)
                     /locus_tag="IWD16_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9088008.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            complement(17792..18085)
                     /gene="rpmA"
                     /locus_tag="IWD16_000927"
     CDS             complement(17792..18085)
                     /gene="rpmA"
                     /locus_tag="IWD16_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP9088009.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(18098..18442)
                     /locus_tag="IWD16_000928"
     CDS             complement(18098..18442)
                     /locus_tag="IWD16_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP9088010.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(18469..18777)
                     /gene="rplU"
                     /locus_tag="IWD16_000929"
     CDS             complement(18469..18777)
                     /gene="rplU"
                     /locus_tag="IWD16_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP9088011.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            19059..19700
                     /locus_tag="IWD16_000930"
     CDS             19059..19700
                     /locus_tag="IWD16_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9088012.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(19762..21393)
                     /locus_tag="IWD16_000931"
     CDS             complement(19762..21393)
                     /locus_tag="IWD16_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP9088013.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT         6205 a         4760 c         3587 g         6963 t
ORIGIN      
        1 ttacacaact gtttcatttt atttaaattt atgcagttat attctatatc ttacaaacac
       61 attccatgta ttccaattcc ctatccgtta taataaaaga aacaggcaac aggaggaaaa
      121 tcatgatcaa atattttaca ttagataatg agaaactgcg taataccgga aaggaaactg
      181 accaaacgat ctggtattgt gtagaacgtc ctaatcaaga agagattcat cagctgacac
      241 atcaatttga aattccgtca gattacatca cttccatatt ggacgatgca gaaaattccc
      301 gtacagaaga attcaatcaa tcaaagttga caaagccagc gttattattg ttacaatatc
      361 cttttgctaa aacaagtcct agcggttatt ttcaagtgga tacatacccg ctctctatta
      421 ttttgacgcc caaaaagaag cttatcacaa ttacgaatca tgaaccactg tttttaaaga
      481 aagtctttag ccagaccttt ccaaaaagtg atatttcgcc taatctcaat atcttctttc
      541 aattcatttg gcaggtcaca ctctcttaca acagttattt agctacgctt caatctcaat
      601 gcgatacttt ggaagccaaa cttcaagtat ccactgaaaa caaacaactt tatcaaatca
      661 tggatatcca aaaaagcctc gtttatttca gagaagcgac ttctgctaat ttagatgcat
      721 tgaaagaact agcgcaaaat aaacttttcc aagaaaatca tgctttgaaa aatcatttac
      781 gtgatgtatt ggtggaaaca gagcaggcaa tgactacttc tcgcattcaa ttaaaactag
      841 tagaacaaat gaatcagact ttttcagcaa tcgtatcgaa taatctgaat aatattatga
      901 aaattttaac ttctttaacg attattttaa ctataccgac aatcgtcggc agtatctatg
      961 gaatgaacat taagttacct attgctgaaa gggatgatgc ctttatatgg ctagttctat
     1021 taacattatt tatttgtatc gtaactacct attatttaaa aaaagggaag tttttataaa
     1081 aaagccctct tgtggaaatg gaaaaaagat tgtacacttg aatcaactaa ttaatgacga
     1141 ggttgaaaac aatgcgtgaa aaaaaacaaa caagtacttt tgctaaagta acaaaatttg
     1201 tcatttggac aatgctgatt ttaacgattg gatctgttgt attgactgct gtaatgagtg
     1261 tcatgtaaaa ataaaatata gctatcagct gctgaaaagg acctgaacca ctgcgaggtg
     1321 gctcaggtcc tttttgcgag tttcaatagt tcttttgcat gttcgagtgt gagttttgta
     1381 atctcacttc cagccagcat acgtgcaatt tcttcttctc tttcttttgt tttcaactct
     1441 gaaacagaag tttgcgtacg tccatcaatc actgctttca caatataata ttgatagtcg
     1501 gcgactgcag caacttgagg cagatgagtg atgcacaaaa cttgagagta tttcgatatt
     1561 tgagaaattt tatctgcgat ggcctgtgca actcttccgc tcacacctgt atccacttca
     1621 tcaaaaataa tacttgtcac accttgctca gaagaaaaaa tagttttcaa agcaagcaac
     1681 atccgagaca gttcgccacc agaagcgact ttcaccaaag gcttcagtgg ctctcctgga
     1741 ttggtcgtaa tgtaaaattc gatttggtca aatccatcat gattcaattg tctattttct
     1801 tcttttaaga aacgtacctc aaactcagtg ttttccatat acagactttt caattcatgc
     1861 agaatggatt tttccaattc ttttgcgatt tttttacgcg cttcatgaag atctgcggct
     1921 tgttgctgaa gtagaagctc tttttgctct agttcctctt tcattttgtc caattgacct
     1981 tctgaaaaat cagaagaagc tagctcttcg gtgatttcgt catagtaagc caatattgac
     2041 tcaatggatt cgccgtattt acgtttcaac tgtcggatta attctaaacg ttgcgtaact
     2101 tcttctaaac gactctcatc taactccaat aaatcaatct gacgactcat atcgccgatt
     2161 gcatcttgca gcaaatagta agcactttgg atattttcat aaattgcttc atactccaca
     2221 tctagatggg cgatcccttg cagttctgaa acggccaagc caacaccatc tacactgttt
     2281 tgttcattat tcgtcagtgc tccgtaacct gcagctaatc catcagcaat tttttgataa
     2341 ttgctaagtt tttctctttc ttcttttaat ttctcttctt ctccgacttg caaatcagca
     2401 gcagctattt cttcttgctg gaaatggagc atatctattc tctgaacata ggatttttca
     2461 ttttgctgca cttttcgtac ccttttttcc agctcacgat aagagatata ttcttgctga
     2521 taactctttt tcttctgttg gaaagctaca tctccaaaac gatccaataa agcaagatgt
     2581 gattcaggct ggagcagttc ctgatgttcg ttttgtcctt ggatatctac taaataactg
     2641 ccgattcttc tcagattagc taatgtgata atatgaccat tcactcgaca aacatttttt
     2701 ccagttaagg acatatcccg tcgaacaatc agattgtcct catctgtttc gatccctaat
     2761 tcaaccatca gttcagagaa tccttcttgt tttggtagtt caaacaaccc ttcaagcaca
     2821 catttttctg ctccttgacg tatataatcg cttgaacctc ttcctcctgc taacagacct
     2881 aaagcatcga taataatgga tttacccgca ccagtctctc cggtcagcgc ggtcattcct
     2941 tcgtgaaaag aaagccgcaa ttccggaata atcgcaaagt tagtaatact gatttcttgt
     3001 agcatcacat tcgctcctca acttaaaggt aatgcaggaa gtctttcttc aataaaactg
     3061 cctggtcttc agttcgtgta atcatcaaaa cattatcgtc atcattgata atcgaaaata
     3121 attctttctt gtaacgttta tcaatcaaat ttgctgctgc cgaagcattt cccggcaatg
     3181 ttttcaaaat aacaaatttt tccatttggt caactgccac gaaagcgtct tttaatagtt
     3241 tttccaattt agtatttaca tcttcgtttg tttcagctgg tatgctataa cggtagccgc
     3301 catctgaagc cggtaccttg atcagtttca gttcctttat atctctggaa atcgtagctt
     3361 gagtaacaga tattcctttg cttttcagga tttcaacaaa ttcttcctgc tttcggatat
     3421 cttgctcact caacaatcga gtaatcaaac gatgccgatc tttttttctc attacgtctt
     3481 cgctccttag catattcttc ttaatcaatg atagcttatt tccttggtat aataaagaag
     3541 aatccgcata cttctcactt gattttcaca gttacaattt atttatggtc aaactgttcg
     3601 tgtgcatttg aaaccacttc tgccatatta atagctgaat tgattgttcc gctttcgggt
     3661 actttcctca agtgagccaa gaactcgata tttccttctc cccccgtgat cggagaataa
     3721 cttaattcca gcacatcata tccaccttgt gccgcaaatg acaaaatgtc ttctaaaact
     3781 ttttgatggg tctgagggtc acgaacaatt ccgtttttcc ctacagcttc tctgcctgct
     3841 tcgaattggg gtttgattaa agcaatcact tctccctctt cttttaaaat agcatgtaat
     3901 ggaggcagca tcagttttag tgaaataaaa gaaacatcga taacagcaac ttctggaatt
     3961 cctaaggtaa agtcttcggg ttttgcataa cgaaaattca ctctttccat tacgacgact
     4021 ctaggatctt gtcttatttt ccaagctagc tgattgtagc caacgtctaa agcataactc
     4081 atttttgcgc cattttgcaa agctgcatca gtaaatccgc cagtggatgc accaatatct
     4141 aacaatgttt ttccttcaac agatagatca aatgctttga gtgctttttc taattttaat
     4201 ccccctcgag aaacataagg aagtttttta cctttgactc ttaattcact actcactgga
     4261 attttttctc ccggtttatc aaaccgctcg tttttttcgt tgtaaatcaa accagccatc
     4321 actgaacgtt tggcttgttc tcttgtttca aataatcctt gtttgaccgc taatacatct
     4381 acgcgttctt tttccattct ttccctcatt tctccacatc aaattgcttg atcatctggc
     4441 gcatcagccc gccatcaaaa gaaaaaacgt cctcttctaa tttgtctatg atcttgttgg
     4501 cggataacag ctcaatctcc aacgcctcac gtgtcttttc taggcctaat aaacgcgggt
     4561 aagtattttt ttccatacgt tcatctttac cagctgtttt ccctaatgtt ttcgttgtac
     4621 caatcacatc gagtagatca tctcttatct gaaaagctaa acctaaatgt tcggctaatc
     4681 gttgaagtag caataagatt tcttcaggtt gcttcgctaa tatgccacca gccagcaagg
     4741 cataacgaat caaatgccct gtctttcttt catgaataaa tgctaattct tctaaagaaa
     4801 gctttttcga ttcgccctcg atatctgcag cttgcccagc aaccatacct tgtgatcccg
     4861 cacaaacagc taattgctga agcagcagaa gcttaggaga attacctaag tgtgccatac
     4921 tgattagctg gaatgctcct gtaagcaaac catctccggc caatatagct aatgcttcgc
     4981 cgaatacttt atgattggta ggttttcctc tacgaagatc atcattgtcc atcgcaggca
     5041 aatcatcgtg gatcaaggaa tacgtatgca ccatttctaa agcagctgct acttgataga
     5101 cgggaacatc aataggctca tcaaaggaag caacggccgt taaaagtaat aagggacgta
     5161 ttcttttccc gcctgctttc actgaataac tcatcgcatc atagagtcct ctatccgttg
     5221 tatgctcatc taaaaaagac aagatttctt tttctacagc aggtagatga atagctgaaa
     5281 agtcagttaa tctcatctca gctttcctcg tttccgtcaa aatcaacttc ttcatttgat
     5341 tctgtcatca ttttcgtcaa ggtctcttct gctttagcta atgtatcttg gcaatgtttg
     5401 cttaattcca ttcctcgttt aaacgaatct agagctgatt ctaatggaac gtctccttgt
     5461 tctaactgca tgacaatttt ttctaattct tgcagtgact cttcaaaagt tggattttcc
     5521 atatttttat tctcctttgt ctatctcttg tacttgtcct ttaacgattc catcttgata
     5581 atggatcgtc agcaaatccc cttgatgaat ctcggtgaca ctttttacta cttgatcatt
     5641 taacgtcgta tagctgtagc ctcttcccat gattttcaat ggactcaaca aatcaagaga
     5701 ttgaacagct tgagcaaaac gctgctgctg ttgtttcata tattgttgga ttttctcttc
     5761 taagcgtttt tctagataag cagtttcttg gcgagctgct ttaactcgat aaagcggagc
     5821 cacttgttcc aattggtggc ttaatgcgat cgtttgtttt tctttcgcat gataaatttg
     5881 ctgtgtagct tgatataatc gctgtttcaa ctggtctaat ttgatggtct gaccttcata
     5941 caatctttca ggctgccgaa atacatagga acgtcgagca cgctcgaacc gttcttgttt
     6001 gcgctggatc tggcgtacaa acgcctgctg caatctagct tgccgttctt gaatgcgcat
     6061 caactcttct gacaaaacag gaacagctaa ttctgcagct gctgttggag tggcagcccg
     6121 aacatctgct actaaatcag cgatcgtggt atctgtttca tgtcctacag atgaaatcac
     6181 cggcgtatta gcttgataaa tcgcccgagc aacccgctct tcattaaacg gccataaatc
     6241 ctcaatcgaa ccacccccac gaccgatgat cattgtatcg aaattgccca tctcttctac
     6301 tctctggata ttcctgacaa cattgtccgc tgcttggttt ccttgtacaa ctgttgggaa
     6361 caaaaccaat tggacaattg gatatcttct tttcaccgtc gtgatgatat cgcggataac
     6421 cgctccgctt ggactggtaa ggaccgcgat tcgctttggg aaacgtggca gcattttttt
     6481 aggcccttga aaaaggcctt ccttcatcag cttttctttt cgttcttcca attcttgata
     6541 taaagcacct attccatcag gctccatgtg ctctacatag atttgataag aacctcccgc
     6601 ttcatataat gaaatacgcc caacgacaag gactttcatt ccttcttttg gctggaatcg
     6661 taatttttgg aatgctcctt tgaacataat agcagatatt tttgcatggt catcttttaa
     6721 gctgaaatat tgatgggcat tggcacgtaa acggaaattc gatatttcgc ctgttaaata
     6781 tacgcgttct aaataaggat cggcatcaaa ttttcttttc aaatacttcg ttaatgtagt
     6841 gacagttaaa tattcttgtg ccatctattc acctgctcca aaagcttcgg cagattttac
     6901 ggtttgatac atcagcatcg tgatagtcat tggccctaca ccctttggca caggagtgat
     6961 ccagcctgca ataggctcca cttcatcaaa tttcacatca ccgatcagct taccgttagc
     7021 atcacgattc atccctacat ctatcactac agcacctggt tttacgaatt cttttgtgac
     7081 aaaatgtcca cgtcctattg ctacgactaa aatatctgct tcttgtgcca gttcagccag
     7141 attttcggtt ttagaatgag caacagtaac agttgcatct gccatcaaga gtagctgagc
     7201 catcggttta ccgacgatat tgcttcgtcc gataactact gctcgtttac ccgccggatc
     7261 gatagaataa gcttcaaaca ttttcatgat gccgtatgga gtacaaggaa tcttgtccgg
     7321 tttacccgca aggagtctgc ctaaattcat cggatgaaaa ccgtctacgt ctttttcagg
     7381 gcggatagct aacaggatct tttcttcatc tatatgcttt ggcaatggta attggaccaa
     7441 tataccatga aaactaggat cttgattgta tttttcaatt tctgcaagta gttcgtcttc
     7501 agaaatcgtc tcaggataac gctctaccaa tgaatggatt ccgatttctt ttgctacacg
     7561 ttctttattt cttacataga tttggctggc tggattttcc cctaccaata aaacgactaa
     7621 tccgggatgg ataccattat ctttcaattt ttggattttt tcagcaatct ctgcctgcat
     7681 tttttcagct aattcttttc cgttaatcaa ttctgccatc taaccactcc cagatatttt
     7741 ttgtgttttc attctaccat agaattttcc cgatttataa caggtttatg atgattcata
     7801 gaaaaaagct aggacttacg ccccagcttc taattctttc aatacattgg ataaaacacc
     7861 gttgacgaat ttacgtgaac ggtcatcact aaatgttttt gccaactcga ttgcttcatt
     7921 taaagcaaca gccgctggta catctgtcac ataagtcatt tcaaaaatcg cgatacgcaa
     7981 gatcaccaaa tccatttttg agatccgatt gatgtgccaa ttgtttttta aatgtttttc
     8041 aatcacttta tccaattcat ctttttttgc acagacaccg ccaactagcg tatcaagata
     8101 aacaggaaca aactctgatt catcttcgtt gatcatgtcg cggtggtcta attcgattgc
     8161 gttgaaaatc gcatcttctt ttgttaggtc tgcattgaag tctaatggaa ataaagcctg
     8221 caatgccatt tcccgaatct cgtgtcttga taattcttta ttcattctct tcctcttcgt
     8281 cttcaaaaag ttcttcgatt gttggttgtt ctaatttttc aggaacaaca gctactacat
     8341 ggatattgac ttcagataaa tcgatatccg tcatgaaaag gacttgctgg ctgacccgtt
     8401 cctgcatttc cattgctact ttaggaactg aaacaccata ttctagataa cagtaaagat
     8461 ctactttcaa gccatcttcc tcgtttgcta agtaaacacc ttttccgtga gctgcacgtc
     8521 ctaataattc agtgacattg ctggcaaaag ttccgcgcat tccgtatacg ccttctactt
     8581 tagaagctgc aatcccgata attacttcga tgacttcagg agcgatcacg atctctccta
     8641 aatcttggtt tgcgtttaaa atcaaatttt tttcatctgc catttattta ttcctccctt
     8701 tgcctaagca cgagatacat ccgaattctc tatgatgcgg catagtgatc acgtggtaag
     8761 aaaactgccg ccgatcagct gcaaactgct atgaattgga gaaaacaccc aactttatta
     8821 tctcttacag cttaaaagtt cttctatagt gtatcattaa atcgagtgat tttctataac
     8881 aaagtctgtg aacactgatt tttacgctta cttcttctat tgatgaaact ataagatgat
     8941 caattctttt ggtgaacgag tcaacacacg atttccatct tctgtgatca acaagtcatc
     9001 ctcaatacgt actccaccga ttcctggtaa gtagatccct ggttcatcag tgatcacatt
     9061 tcctggtaca aattgctttt ctgcacgaga agaaacgttt ggtccttcgt ggatctctaa
     9121 accgatccca tgtcctgtag aatgtccaaa tgcttccccg taaccatgtt ttgaaatata
     9181 atctctagca actgcatcta attggacacc agtcattcct ggttttgcag catcaataac
     9241 tgctaattga gcatctaaga ctatttgata aatttctttt aatttttccc caggatcacc
     9301 aatagcgaat gtacgtgtca tatcggaaac gtacccttcg taatagcaac caaagtccaa
     9361 tgtgatcaag tcaccttgtt cgatgatttt tttgcttgct acaccatgcg gcatagccga
     9421 acgcaatccg ctagcaacga tcgtatcaaa agaaacacct gaagcgccaa gagaacgcat
     9481 ataaaagtct agttgatttg cgacttcaat ctcagtcatg cccggttgga tcattttcag
     9541 gatgtgatca tatgccatat ccgcaatatg acatgctttt tcgataatag cgatttcttc
     9601 ttcatctttc acttctctga gttcttcgat catctgtgaa acaggtacta attccgcatc
     9661 gatgatttct tctaaaaccg aatattctaa aaatgagact gttgtttcct caaaagctaa
     9721 tgagtgcagt tcttctttgc gtactaagtt tgctacctca tcaaaaatag gtccgacatt
     9781 tttaataatt tcaaaccctt gagcctgcgc tgccgcctgc tcggtataac gaaaatcagt
     9841 aataaaaaat gctttttcca aagtaataac agctaatcct gtcgtacctg taaagttcgt
     9901 taagtaacga agattgtacg gactggtaac caaaaatgaa tccaaatttt cttcttgcat
     9961 ttttttgcgc aatttttcta cacgtagcat cataatgaaa ctccctcttt ctttgcacgc
    10021 ttttttccat tatagcaaac ttttcttcgt acacgacagt taaataaaaa tttccggaaa
    10081 acttcttttt attttaaata aataaagcca aaaaggagtc acagtctcgc tgcaactcct
    10141 tttagtttac tattttgtaa aaacttatga tcgttttcca aagaaaaatg agacaactgc
    10201 taccaaaatc actgcgccta aaatagatgg aataagtgcc atacctgcag cttttggtcc
    10261 ccattcgccg aaaatcgctt gtccaatact tgaaccgact agtccagcaa tgatattcgt
    10321 tacacagccc atcgaatttc ctttactggt gatcgcacct gcaattgctc cgatcacggc
    10381 tccaacaatc aatgtccata accaactcat aatataacct ccttctttgc taatcaattt
    10441 tagcatatgg gatttaaagg ggacaaataa ttggttttgc tatttcatat tttcttctgc
    10501 aaagtaaaaa aataaagaaa acctcctctt agaagttttc tttatttttt acttgcgttt
    10561 aaacatacta cgaataccac gtttcactac atcaaagaaa ctcaatgatt gtaccattct
    10621 atcttcatct acatattctc tgatcgcacg aagcactttt ttaggttctt tggaagaaaa
    10681 tgtgtatgta ccattttttt tcgtttgaat ggcatatcga ggaatccatt ttccctttag
    10741 catgactgat gctagtacat aatccacttc ttcccatgga atttggatat atttgcgtct
    10801 atctcgcgca tggtaaaact caaaaccatt atcacctatc atgatttgcc cgtaatctgt
    10861 cagacccgta aacgaagtag catcaattac caagtctact tttgtattga ttgattgaac
    10921 cattttctcc ctccagatat ttgtttatga acagtatact atataaaaaa agctcgggac
    10981 aacgcccgag ctatattttt atggattata aaagtccgat gacgtgtcca acaacaccga
    11041 ctacgaataa accaaggatg ataacgattg gagaaacctt tttcttaagc aaccacatac
    11101 ataacaatgt taatcctaat gcagctaaac caggaatcaa ttggtctaag ttgtcttgta
    11161 atgttgttac cttttcagga gaaagggcta agccattatt aacttgttca aatgcactct
    11221 tgattccttc accacctgaa ggcaatttat cccattcgat gtaagcacct ttatctaatt
    11281 ttacagttga tacaactggt aagaatttga ttgataccca tctttgtaca agggcagcta
    11341 atacgaacat ccctaaaatt gaagcacctt ttgttacgtc ttgtaacaat ccgccagaaa
    11401 ggtcatcagt aattttagaa ccagctttgt agccaaattc ttgtgtatac cacatgaatg
    11461 cccaacgaat aatgttccaa cctaagaaga agatgattgg tccaaggata tttcctccca
    11521 tagctaatga agcacctagt gcacctagca ttggacgtac tgtaaaccag aacactgggt
    11581 caccaacacc agctaaagga cccatcatac ctactttaac cccttgaatc gctacatcat
    11641 caacaggcgc gccattcgca cgttcttctt ccagagccaa tgtaacccct aaaattggag
    11701 aagcaatgta tgggtgtgta ttaaagaatt ccaagtgacg ttttaatgct gcagaacgtt
    11761 cttcttttgt tttgtataat tttttgattg ctggaatcat agaaaatgcc cagccaccgt
    11821 tttgcatacg ttcgtagttc catgaacctt gaatgaaggt agagcgccac gcaacagcta
    11881 aacgatcttt ttttgataat ttgatttttt cttctgccat tttattttcc tccttcaaga
    11941 ttaatagtca ttcaaaatgt cgcctaaagg gtcaccagag ttgcttccgc ctccattatt
    12001 tgatgaacca cccattttag aaaggttcaa gtaaataaga gctaatgcaa cacctaaagc
    12061 acctagagca atcaatgtta attgagaaat tgcagctaca acgaaaccaa taacgaagaa
    12121 tggccatact tcttttgtag ccatcatgtt gattaccaaa gcgtaaccta cagctacgac
    12181 cattccccca ccgatagcca taccatctgt taaccatgct ggcattgatt caaggaatga
    12241 ttgcacagtt tgtgctggga taaatagcaa cgctgctgct gggaccgcga tacgaacccc
    12301 ttgtaaacat acagcaatga tgtgccacat ttcaacctta cggatgtttc cttcttcagc
    12361 agccgcatcc atcaaatgaa cgattggaac agcgattgta cgtacgatca ttgttaagaa
    12421 caaccctgca acagccaaag gaacggcaat cgcgatcgca gaaggaacac ctttaacacc
    12481 ttgtccacct aaaactaaaa taattgctga tgctactgat gctaatgcag catctggtgc
    12541 tacagcggca ccgatatttg cccaacctaa agcgatcatc tgaagcgtac cgccaagtac
    12601 gataccagct tcgaggttac cggtaactaa accgatcaat gtacatgcca ctagtggttg
    12661 atgaaattga aattcatcaa gaataccttc cataccagct agaaaggcaa ctaatacgac
    12721 taaaattatt gaaataatag acatgataag cctcctataa attttaatat tatttattac
    12781 gcttttgcta attcagcttt tgcttttttc aaaatatcat ccatgttatc gcgtgaatca
    12841 ttaggtactt tacgtacatc gaatttcaca cctttttgtt caagcttctc aaacgcttca
    12901 acgtcttctt gtcccataga caatacttta cttacaacga ctttaccgac cgaatgcgcc
    12961 attgacccaa catttaattc tttgatgtct acgccacctt caacagctgt caatacgtct
    13021 tcaggatttt caaataaaag caatgctttg gtatttccaa aacgagggtc ttttgctact
    13081 tcgatcattt tgctaatagg aataacattt gcttttacac ctggaggagc agcttgttcg
    13141 attagtctct tacgcagatc atcctttgat actgcatctg aaacaacgat aatacggttt
    13201 ggttgcactg cttttgtcca agctgtcgca acttgtccat gtaacagacg tgagtcaaca
    13261 cgtgctaata catatttgat ttttccgtct ccgaccacag ttccttctgg aagtgctcct
    13321 ttaggttgtg cgtcttcaac tgctgctttc ggtgcttctg ccggttctaa ttcttccggt
    13381 tttactttta caccgtcttt tgctgtttca atgatgtgtg ctgcaatttc atgagcagat
    13441 tccattgaaa agcgtgatgc gtaggcttca atcaacattg gtaaattcaa accgctgacg
    13501 attgcccatt tatctttgtg ctcttcgaat aatgtgtttg attgattgaa cggtgttccg
    13561 ccccataaat caactaagaa taacacttcg tcttgattat caaaagaagc aaccgcttct
    13621 tccaatttgg ctctcaagtc atctggacct tcacttggtt tcaaaatcac ggctttgacg
    13681 ttttcttgtt cgccaaagat cattgaaccg gattgtaaga ttccttcagc aaattgtcca
    13741 tgacttgcta ggataatccc taccatattt atacctccta tttacttttg ttgtaacaaa
    13801 ttcaaaaata ttaataattg ctaaccatta caacagaatt atttgacctt ttccaacaac
    13861 cattcttgct acaccttttt gattttcgct agtagttcca tcaaaaggat tttcctgtca
    13921 gcaggtactt ttcgaatctc taactccact tcttgactgt tcaagtaacg aaaggaatca
    13981 atatcttgtt gatttactga aacaaaattg gtaatcattg atttgccttc agtaaaactc
    14041 ataccgccga tattcaacga cttcaactgt acaccgcagc ggacaaccgc ttcaacatct
    14101 tggggattgg tgaataaaag catcactttt gctcgtgata ataatccact atgatagatt
    14161 tgaatcattt tctgcacggt tattacattt gctgtgatcc ccggcggtgc agcttctttt
    14221 agtagaaact ttctcaattt atcttgagcg actacatcgc tgatcacaat gatacgttca
    14281 acacctgttt gcttcgacca aactgttgcg acttgcccgt ggattaaacg atcatctata
    14341 cgtgccaatc gaatatccat ctgcaaaact ccttcttcct cactacttca cagaaacccc
    14401 tatcatttct gtgtgaacaa ccacctccta gcacacacta ctataagcaa aaagcgtgcc
    14461 aactttttta cgatacacta attgaattta aaggattctt aaccaataca caaaaacgat
    14521 acactataaa agtgtatcgt ttttgtgtat tcatggcaca gtgtattctt ttttatctaa
    14581 ctgattatgg actagacgta gcagataata catttcttct atcggcactt ttacttttat
    14641 ctgatcttcc aacagaacaa ctgaggctgc caattggcta taaagaggat cattcgttgt
    14701 atttgtcaat tcgttttctt ccacagtcaa tggttcattc cgaataatac gttcgatcat
    14761 tcctgctgtg tgcatcacta ggttgatttt gaatccatat tcttcatcgc cgattcccgt
    14821 cgtctgagag atttgtgtag aaaattgcca cattggatcg attaacttac taccattaat
    14881 aaagatgaag ttatcagaga taaactcaac acacgtattt ttagctgact cttcatctag
    14941 ggagatgaat tcagtttcct ccaattctga ttccatcagc aactgatcca aaatgacttc
    15001 gatattttga tcaataaaac gttctaaagg aataaatggg gcttggatat tcaaatcagt
    15061 aatcccagta gtagctatga tctgatattc tttttggata actggcagtt cttctttcac
    15121 ttccacaatc gacaaagcta gaacttctaa ttctgtttct tgacgcttgt ttaccgctcg
    15181 ttcgatcaat tctttgattc tttgtgctgt accttcgcca gatgcgcaga tagccaaaac
    15241 agcttttttc ttatgaatag gttttgcttc ctgaacagat tctacagaag atatttctgc
    15301 gtaacctcga aaatttttca atgattcatg caacatatct agatctgtcc ctaatactga
    15361 gacctttcgt acagtttcca aaactagaga agttgttacc atatcaacag tacgtactgg
    15421 aattccagtg tctctttgaa tttgcgaatt aaacgacgca agggacccca tatctactaa
    15481 gagtatgacc ccgcttcctt catctacttc ttgtacattg cgttcaattc ggtccaatgc
    15541 tgttttcgga tccatatcca aaggcatgtc tactgctcgt acgttctctg catctaacaa
    15601 ctgttggaca acttggacca tactgcttgc tgtgctgttt ccgtgagctg caacgactac
    15661 accgattctg ccgcttgctt gatttgcccg taacgagacg agtagaacgg tcaagtaata
    15721 gtcttcactt tctggaatct ccacttgaaa atacgtttcg atataactac gcacttcttt
    15781 cgcaacagca tattcatctg gaaaatcaaa agccatctca cggatgttat cattcgtatg
    15841 ccgttcttct cctaagttga tttttttcaa aaaagaactg atatgcaagc tcatagcata
    15901 aataaagttt tgctgaaact catatggtaa agatcttttg accattacat aaatctgatt
    15961 ggtcacttct atgattttcg gatctacaaa ttctgctaat ttgctgtctg cattaaaaga
    16021 aaaaccatgg tctttataga aagacttcag atggatgttg atatctgtag aaatgaaatg
    16081 attgattgct tcttgatcca aaccatccga cttcagtaaa gctgctttat ctccgatgat
    16141 atcatataga ttataaggta actcatagga gtccgattgg attttcatga tcgtatcatt
    16201 tggtgctaca gttatctttg gctccaatat tttagaaagc tctgccattg cactgcggtt
    16261 gcttgctaat tgaatcaatc cgctacgaat cccttctgtc aaatcatcga ttgtgatcga
    16321 gatttcgggt gattgcatat gatttagaaa tcctcgtgca catactaact ggatatttga
    16381 cttcaattgt cctatatttc cataggtcac actgccgatc aatgcttttg ctacatcttc
    16441 tgtcaaagaa atctttcgct gcgtgcggtt tgcttcgtgg gcaatcatga tttttactaa
    16501 atcgactttc tcatttgcag gacgtttttc aaaagccgga agtttgatat tgattgggat
    16561 acgccgaaca aaagtttcta atagagaaga acctgggtct tctgtggtcg cacctacgat
    16621 ccgtacattt gcttcatggg atttcgttgt ttccccaaga cgactgtaag ttccatgatc
    16681 cataaagtaa aagatcattt cttgaccttc aggcggcaac cggtgaatct catccaaaaa
    16741 cagcataccg ccatccgctt gatcgataat ccccattttt tcttcatctg cgcctgtaaa
    16801 agcacctttt gcgtacccaa ataaatgact catcaaaagc tcagggttat tggcataatc
    16861 agcacaattg aaaacaatca gttccgtttc ctcatctatt acatgatgcg cttttgcaaa
    16921 atgatacatc gcgtgtgcaa aataagtctt tcctgatccg gtaggtcctg taatcaaaca
    16981 atttaatcct cttggcggat acaaaatcgc agcttttgcc tgttccactg aatttttcat
    17041 gctgccgtta gctccaatga tttttgcaaa gatatcctta gtatctattt ggagtgtatt
    17101 tttttcttca gagaccggcg tttctttgta actgactaca tgttttgtaa ctggctttac
    17161 gtgcgtaacg agtgtttgat atatgtaacg tactggtcgt ccgtctgtct ttatcagttt
    17221 tccttcgcgc actagctgat tcagatcttt actgctattt gttcgctgaa tccctaacac
    17281 ttcagcaatt tcctgtgttg ttactccttg atcatattct gcctttgtta aatgagcggt
    17341 tttttctttg acatacatat aaattcggtc aatgcgtttt gccaatggta tcgctccttt
    17401 tctatagact attgtactta ggcttcaaaa cataatacta tgtatttatt tctgtttcgt
    17461 caactattta cctgattttg ttttgttgat aacacgcgga tagatagaaa tgttcaattt
    17521 aaaagaacga aaaatcccga tacgattttt ataatggaaa caagaaaaag aacactgttt
    17581 accatataat tatttcactg atagcaaaca atatagcttc catcaattga aaacgatagt
    17641 tatcaaaaca tcctgattcc aaattggcaa aaaaatatta agtgtaaaaa gacaaagtaa
    17701 acttcgtagc ctccactaca acttttattt ttataaacaa aaaaggctgt gacaaaagtc
    17761 ttgtcacaac ctctttaatc ataagattca attaagcttc ttgagctact ggataaacag
    17821 acacttgttt tttgttacgg cctttacgtt cgaaacgtac tacgccatca acttttgcaa
    17881 ataaagtgtc gtctccaccg atacccacgt ttacacctgg atagattttt gtaccgcgtt
    17941 gacggtaaag aattgatcca cctgtaactg tttgtccatc agcacgttta gcacctagac
    18001 gttttgattc tgagtcacgg ccgttagatg tagaaccgcc accttttttg tgggcaaata
    18061 attgtagatt catagataac aacatggtct gcacctccta tttatcatta attgttttaa
    18121 tttggatata ttctgagttt tcagcttcta tcgattgtaa tcctaataaa agattttcta
    18181 atagaatctc agcaatattg ttttgttctt gtgtcattcc tgaagtgact tcggtataaa
    18241 gataaccgcc ttcttcttca ttcatttcaa caatcggctc aaaaccggct aatgaggcaa
    18301 tcccgttgac agtgctgatc gctaacgcag aaacacccgc acaaacgata tcacttccat
    18361 aaggaccagc atcagcatgc ccggtcaaag tgaatgaaac gatctgacct gcgtcatttc
    18421 gcttaaatgt tcctttgatc atatataggc cttctttctc aaacgctatt aagcgttgat
    18481 cgcgtcgata accacttttg tatatggttg acgatgacct tgtttacggt gtgaatgttt
    18541 tttaggtttg tatttgaaag taacgacttt cttttgtttg ccgtgttttt caacagtacc
    18601 ttcaacagtt gcacctgcaa cagttggagc gcctactttt gtagattctc cgcctactaa
    18661 gataacttcg tcaaaaacaa ctttttctcc tgcttcaacg tcaagttttt caacatagat
    18721 tgcttgtccc acttcaactt ttacttgttt accaccagtt ttaataattg cgtacatgct
    18781 ataaagcacc tccttctgat atacttagac tcgccatccg aagtgacagc atatgctgtg
    18841 cttattactt gttctgagcg gttgtagctg tggagtccac aattacaaca gtactagctt
    18901 accagaacag caaccgttag tcaactaaat ttaaaatcaa aaagtaaaac aaaaattcaa
    18961 gcaatacttc tgcttttcta tcctcacagt tctaccaaat aaaatagtcg cattgtgttt
    19021 cttttattct tataataaaa gaaaaaggag gagcataaat ggaataccca aaagaactga
    19081 tacaagaagc ttctttaaga atgaacggcc gtcctgtaga aggatttatc gctgggcaag
    19141 gacctcttca tcctaaactg atgctcgtgg gagaagcccc agggaaaaca gagattgata
    19201 cgcatgttcc ttttagcggt caagctggaa aagaattgat gcaggcactt tcttcaactg
    19261 gcttaacacg agaagaggtg tatatcacca gtgctgtaag gagccgcccg tatcgcgtga
    19321 ctcatcggat caataaacgg acacaacagt cagaaactgt ttatccaaat cggacaccta
    19381 ctcgttccga agtatttgcg catgcaccaa tattggatta cgaactgatg aaggtccagc
    19441 caaaattaat tgcgacactt ggaaacattg gtcttcaacg tttacttggc aaagaatata
    19501 cggttaccaa aaatcatggt caactctata cagggcctgt cttacaatta acagaacaaa
    19561 aggatgccta cgaatggtca gaaaaaaact atcgaattct tccacttttt catccagcag
    19621 ctattttcta caatcgctcc cttgtacctg ttatccatca agactggcaa gtactagctt
    19681 cacttttaca gcaatattaa aaagaacaga caagaccata aaatcttcag tttacagtct
    19741 tgtctgttct ttttcattgt tttaatggct aattgcagta tatttcgcat gtaaagctgt
    19801 caacgcatga gcaactgttt gatgttgatg cgtgaatgta tgttcccaca agtgagaaag
    19861 ttgtttttct acttctggct gcaaggaagt atagataatc gtttgtcctc tttgctctgc
    19921 tttttcttga aattcatcaa aaactgcacg tgccgtcaaa tcaatacttg gtacgcctcg
    19981 catggagaaa atgacaccat cgtgatgatt tagttcttcc aatttatttt tgagtttttc
    20041 tgaagacata aaaaacagtg gaccactaat atacactact gtccagttat tcagcttttc
    20101 cgtttctggc agccccattc tttgccaatc gatcgtttct aatgaaatcg tgatagcagc
    20161 gcttttgaca acaaaaaata cacagccgcc gatcaccccg atcacgatag caatacttaa
    20221 atcaaagata actgtacaaa tcatcgtaag gataaacaat agaactgctg accaatattt
    20281 attagtaaat agctccttga tcgtttccca ttcattcata cgccaagccg tcacaatcaa
    20341 aacacctgca agtgctggca ttggaatctg tgacatcact ggcgcaaaaa caagcatgga
    20401 gagcagtaag acaactgcat gtatcatacc agctaatcta gtttgtgcac cagatttgat
    20461 ggccacgctt gtacgggcga tggccgcagt agcaggaatc ccgccaaaga aaggaagcaa
    20521 cagatttcca attccttgcg ccattaattc ttgattgcta tctaagggct tatttgccat
    20581 tcggccagcc gaagcaccac atagcaagct ttcaatcatt cctaataaag caatgctaat
    20641 agctggaact gccacttgtt gcaaggcaga aaagctaaaa tctcccatat tcaaacgatt
    20701 actactgatc aatgtttgtg gaatcttacc cacagttgct actggtaaat ggaacaatag
    20761 cataactgct gttgccaaaa tgatagccag cagcgaacta ggcattcgtt tcgcccattt
    20821 ttttggatag ataaacatcc ctaaaatgac tagccctccc atgatcaaag aggctgtgtt
    20881 tatcggaaat cctatcgttt gataactact tagtttttca ataacgtttg taccttccga
    20941 gtgaacacca aataaattat cgatctgacc taatgcaata atgatagcaa tacctgaagt
    21001 aaaccccgta attacgggtg ctgggataaa agaagtcagt gttcctaatc ggaaaagtcc
    21061 tgctgcaagt aaaaatagac ccgctaaaaa agtagcaagc aatacacctt gcattccctt
    21121 tgttgccgca atagacatca agatagctgc catcgcacct gtcggacctg atatttgata
    21181 aaaaccgcct gataaaccgc caataacgat accggcaatg atagccgtaa tcaaacctgc
    21241 ggccgcatca gctccgcttg ataccccaaa agccaaggct aaaggcaatg ctacagcagc
    21301 tactgtgatc ccagccaaaa gatctttctg aaatcttgaa agatcataac ctgaaaattc
    21361 tttttttagt gaggaaacat aatttttgaa cacctatcta actccttttt catgaaattt
    21421 tctaaaatac cattcattag aatatcatat ttcatgagat aaagtaatat ttttcacaaa
    21481 gtttcctgtt agataaattc ttattttttc tatat
//
LOCUS       DACDMM010000032        21314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDMM010000032 DACDMM010000000
VERSION     DACDMM010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.032"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..684)
                     /locus_tag="IWD16_000932"
     CDS             complement(<1..684)
                     /locus_tag="IWD16_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9088014.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKK"
     gene            941..4330
                     /locus_tag="IWD16_000933"
     CDS             941..4330
                     /locus_tag="IWD16_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9088015.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            4333..5754
                     /locus_tag="IWD16_000934"
     CDS             4333..5754
                     /locus_tag="IWD16_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9088016.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            5751..7628
                     /locus_tag="IWD16_000935"
     CDS             5751..7628
                     /locus_tag="IWD16_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9088017.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            7720..8562
                     /locus_tag="IWD16_000936"
     CDS             7720..8562
                     /locus_tag="IWD16_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9088018.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(8714..9589)
                     /locus_tag="IWD16_000937"
     CDS             complement(8714..9589)
                     /locus_tag="IWD16_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9088019.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            9796..10827
                     /locus_tag="IWD16_000938"
     CDS             9796..10827
                     /locus_tag="IWD16_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9088020.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            10916..11221
                     /locus_tag="IWD16_000939"
     CDS             10916..11221
                     /locus_tag="IWD16_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP9088021.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            11400..13415
                     /locus_tag="IWD16_000940"
     CDS             11400..13415
                     /locus_tag="IWD16_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9088022.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            13417..13701
                     /locus_tag="IWD16_000941"
     CDS             13417..13701
                     /locus_tag="IWD16_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9088023.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            13727..15100
                     /locus_tag="IWD16_000942"
     CDS             13727..15100
                     /locus_tag="IWD16_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9088024.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(15183..17198)
                     /locus_tag="IWD16_000943"
     CDS             complement(15183..17198)
                     /locus_tag="IWD16_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP9088025.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(17220..18434)
                     /locus_tag="IWD16_000944"
     CDS             complement(17220..18434)
                     /locus_tag="IWD16_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9088026.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            18655..19599
                     /locus_tag="IWD16_000945"
     CDS             18655..19599
                     /locus_tag="IWD16_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9088027.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            19895..20839
                     /locus_tag="IWD16_000946"
     CDS             19895..20839
                     /locus_tag="IWD16_000946"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9088028.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         7272 a         3904 c         4008 g         6130 t
ORIGIN      
        1 ttttttatcg atatttatca actgtacata agagtctcca gctttccatt caaatggagt
       61 tccttgggat tctctcatga gatgcacaac aaaatacata ataaagaaag attgctcata
      121 tgggctttct tcctgaaaca aattcatctg acaattattc ttgaccataa tttctttgat
      181 cttctttttt tcgattggaa atggcaacgt gcgtcttccg tagtaactta aaaagaaaaa
      241 ggtaacaaag atacgcaact ggagctcact tttaaggcta gttaactgaa aaccttggtc
      301 aacgatcact tttaataaat agctttctaa aatttgattg cattgtttca acttacgtgt
      361 aaaagaagac cggctgatgg aaattttttc acaataatac tcaatcgttt tacccggatt
      421 gacaataatc tcatttaata aacgactgtt tgtagaactt tgcgacattt ccatcagaac
      481 atttaaataa gctggtacat tcaaatgttc aagacgatac cccaattttt gtgaagattg
      541 aatcgttaca tccgttcccc agcggatttt gatctcttcg atatacctta caaatgtaga
      601 ttctgaacta ccaattcgtt tggcaagttc ttttttagtc atccacttgt cagaaatgga
      661 cagaattcga atgatttttt ccatctttct atagtttcct tccaacaacc tgatcatatg
      721 tttttctccc tcaacccttt tctttcaatg aaacgctcat taatcgaagg aatcaacttc
      781 ataaatgaaa ccaattggta tgaaaattgg tgtgattccc tttagcgccc ttcgtataat
      841 aattatcata ttaaaataaa taaaattatg taacgcctgt ttattttaat aattttcact
      901 attcacgtca aaacagaaaa aaagaaaaag gaggggaaaa atgacgacca cagggaagaa
      961 actgaaagtt attttcatgc tgataatatt gagtttatca aactttgtgc cattatctgc
     1021 aatagcagac actacagatg atccaacagt tttagaaaca atttcagctg aagtcatttc
     1081 ggatcagtct ggaaaaaaag cactgaacat caagctaaat gcgaataaca ccagtgctga
     1141 aaagatagaa aaagaaattg gtctagtcga aaattactta agtgatgtgg aaagaaaaga
     1201 aggagatggc tatgcttatc aggtaaatag cgggaaaatt acgttggaaa tctcatcaaa
     1261 cactaaacaa actatcgatc tgagttttcc aatcgatcca gcactttacc acagccaggc
     1321 aaacaagctg atcgtcgata ataaagaata tgacattatt gatgagacag aaaataagaa
     1381 agatacagat gtgtcagtac caaagccaga cgaaatagaa gaagaatcat caaaagaaaa
     1441 cgaaaattct gtcagcccat ttacattgcc tacattatcc ttgccagctg tgagtgtgcc
     1501 atctaatcaa acgattccta cagaatatac aacagatgat cagggcactt atcctaaagc
     1561 cagttggcaa cctacaggaa atacaaatgt tcttgatcat caaggcaata aaaacggaac
     1621 aaatcaatgg gatggtataa attcttggaa tggagatcct aatgatcgga cccattcgta
     1681 tatcgaatat ggaggaaccg gtaatcaagc agactatgcg atacgaaagt atgcaaagga
     1741 aacaagtaca cccggattgt ttgatgttta tttgaatgct cgtggaaatg tacaaaaaga
     1801 tatcacgcct cttgatctcg tattggtcgt agactggtca ggaagtatga acgacaataa
     1861 tcggatcggt gaagtaaaga ttggtgtcga tcgttttgtc gatactttag cagatagcgg
     1921 tatcacagac aaaatcaata tgggatatgt cggctactca agcgaaggat atagctacag
     1981 taacggtgca gtacagatgg gttcatttga ttcagtgaaa aatcaagtaa aatccattac
     2041 accttcacgg acaaatggtg gtacttttac acaaaaagca ctaagagatg caggaagcat
     2101 gctatccgtt ccaaatggac ataaaaaagt gatcgttttg ctgacggatg gtgtaccaac
     2161 attttcctat aaagtacagc gggtacacgc acaatcaagc agcaattatt acggaactca
     2221 gttttctaat acgcaagatc ggccgggaaa tacttctcta atctcaagaa tctatgatgc
     2281 acctgaccaa aacaatctat ccagaagaat cgacagtacg tttatcgcaa ccatcggaga
     2341 agcgatggca ctcaaagaac gaggaatcga aatacatggt cttggcatcc aacttcaaag
     2401 cgatccggca gctggtctct caaaagcaga agtagagtct cgtatgcgac aaatggtttc
     2461 atcagatgaa aaaggcgatc tttactatga atcagctgat catgcaacag atatctctga
     2521 atacctagcc aaaaaagctg tacagatctc agcaactgta agcaatggac aaataaatga
     2581 tccaatcgca gaaccattca tttatcagcc tggtacactt tcagtcaaga gtgtggggac
     2641 aagtcctaca acggtcactc catctatttc catagaagga aataccatca agagcaatca
     2701 gatctattta ggaaaagacc aagaaatcca aatccattac caagtgagaa tccaaacaga
     2761 aaatgaggac ttccatccaa atttctggta tcaaatgaac ggcaggacaa ctttccagcc
     2821 aaacattgat accaatgaat tagctgaatt cggtatacca tctgctaaag ctcccggagt
     2881 cagtcttcac atcaaaaagt tatgggaaga atttgacaac aatctagctg atcgtccaga
     2941 tcaagttact tttgagattc aacgggaaca tacgacaaat gctgcagctt ggaaaaacgg
     3001 atatattcga atcattaaac cagctaaaga tacaacaaat acgtgggaac gtgcagacat
     3061 tgacaaatta tctgcaaata gcggagaaag ttatcaagag atattatcac tacctcaata
     3121 caataatcaa ggtcaagcat tcagttacca aacaatcaaa gaattacctg taccaggata
     3181 cgattctcaa caaatagatg caatgacatg gaaaaatact aaacaattca caccgttaaa
     3241 cttgaaaata acgaaaaatt cctctacagg tgaaaaggat cttattggcg ctgttttcaa
     3301 attaacagga gattctattg atactttact aacagatcat ggcgacggaa cctattctct
     3361 tccagaaaat gtcaaattgc aaaaagaaat gacctatacg ctgacagaaa caaaagctcc
     3421 agaagggcat ggattaagca aaaagactac ttgggaaatc aagatcgctt ctgatggtac
     3481 ggtaaccatt gatggaaaaa cagtcactac ttccgatgat acgatccagt tgactattga
     3541 aaatcctttt gttgaagttc ctgtagcagt acgtaagtat gcgatgcaag ggacggacaa
     3601 agagataaat cttaaaggag cagcattttc cctacagaaa aaagaagcaa atggtactta
     3661 tcagccaatt gacagccaaa caacgaatga aaaaggtctt gccagttttg attcactcac
     3721 acctggtaaa tatcgagtcg ttgaaacagc tggtcctgcc ggatatgata cttcgccggg
     3781 aaattatgaa ttccaaatcg ataaatatgg aaaaatcatt tacacgggaa aaaataccga
     3841 gatgacaaat aatgtatgga cgctcactca tcaaaatcga ctaaaagcgt ttgatctaac
     3901 ggtacacaaa aaagaagaca acggacagac attaaaagga gcaaaattca gactgcaggg
     3961 accagaaatg gacttagaat cgccaaaaga tggacaagaa acagatacct ttctattcga
     4021 aaatttaaaa cctggaactt atacgctgac cgaaactttt acaccagaag gataccaagg
     4081 tctaaaagag ccagttacta tagttataca cgaagatggg tcaattcaag tggatggaca
     4141 agatcatgaa tctgttctgt caccaggagc caaaaacaac cagatttctt tagacatcac
     4201 gaatcaggca aaagtaccat tacctgaaac gggaggaatt ggccgtttag gaatctatct
     4261 agtagggatg attggttgtg cgttttctat ttggtatctt tttttgaaaa aagaaagagg
     4321 gggcagctaa atatgaaaaa acttggttgg cttagtatgt gtctcttctt gttactattt
     4381 aaaccagctt ttactcaggt agcaacagaa acagaaacag aaatggttca gattacttta
     4441 cacaaattgc ttttcccaaa cgggcaactg ccgaaaaatc atccaaatga cggacaagaa
     4501 aaagctttat tacaaacgta tcgaggatta aatggtgtca cattccaagt ttatgatgtc
     4561 acagattctt tttaccatct acgggaaaag ggcaaaacgg tagaagaagc acaagcagag
     4621 atcgcaaaaa acggtgcgtc ttccggtatg tttaccgcag aagcaacaac tacaactctt
     4681 aacaacgaag atggtatcgc ttctttttct ctggccgcta aagatcaaga aaaaagagat
     4741 aaagcgtatc ttttcattga atccaaagta ccagaagtcg tcaaagaaaa ggcagagaat
     4801 atggtagttg ttcttcctgt acatggacaa aacaatcaaa aactttcaac tatccatttg
     4861 tatcctaaaa atgaagaaaa cgactaccct gatccacctt ttgagaaggt attagaagag
     4921 cctagaaatg attttacgat tggtgaaaaa atcacttatt ccttgcatac gacaattcct
     4981 gtaaatatcc ttgactatca aaagttcgaa ttgtcagata gtgcggatga agcattaacg
     5041 tttttaccta atagtttaac gatttcatcg aatggagaaa agctgacaga aggctttgtc
     5101 atacacaaga aacctcacgg atttgatgtt ttattttcga tcccttcgtt ggaaaaatat
     5161 gctggaaaaa aactgaccat ttcttatcag atgcagctaa gcagtacagc acaggcgaac
     5221 aaggaaatca acaacaacgg aacactggat tttggttttg gtgtcagtac aaagaaagtc
     5281 tctgtatata cagggagtaa gcaatttgtc aaaatcgaga caaataaacc agataaacga
     5341 ttagctggcg cagtattcct tattaaaaac aaagcaggaa attacctcca gcaaacagcc
     5401 aacggataca agtggacaaa gaacgaatca gatgcgcttc acctgatttc cgataaaaat
     5461 ggcgcttttt caatttccgg gttgaaaaca ggaagttatc gattaaaaga gatcgaagca
     5521 ccttctggtt atattttaag tgaaacagaa attccgttta ccatttcaac ttttctttct
     5581 gaggataaag aggcggacag tatattgaaa gtagtcaata aaaaagaaaa tagccgtcca
     5641 tttcttccaa aaacaaacga aacgaaaaat acacttttag gcgttgttgg tatggtattc
     5701 gcaagctttg caatctggtt gtttatcaaa aaaagaacag gagtgaaaaa atgaaaaatc
     5761 ataaaaaaat aaacgttatg ttaggagtcc ttttccttat tttaccatta ctcacaaaca
     5821 gcttcggcgc aaaaaaagtg tttgcagagg agacagcagc tcaagtcatc cttcataaaa
     5881 agaaaatgac tgatttaccc gatcctttaa tccaaaacag cgggaaagaa atgagcgaat
     5941 tcgatcaata ccaaggatta gccgatattt cattttcagt ttataacgtc actcaagaat
     6001 tttatgcgca acgagataaa ggagcgtccg tggatgcagc aaaacaagca gtccagtctt
     6061 tgactcctgg tacaccagtt gcttcaggaa cgacagatgc tgatggaaat gtcactttat
     6121 ctttacctaa aaaacaaaat gggaaagatg cagtctacac gatcaaagaa gaaccaaaag
     6181 acggagtgtc agctgccgca aacatggttt tagctttccc tgtatatgag atgatcaaac
     6241 aagcagatgg ctcttataaa tacgggacag aagaactaga tactatccat ctctacccta
     6301 aaaatacagt cggtaatgat ggaacgttga aagttacaaa aatcggtact gccgaaaacg
     6361 aagcactaaa tggagcagaa tttattattt ctaaagaaga aggaacacca agcgtcaaaa
     6421 aatacatcca aagtgtcaca gatggattgt acacttggac aactgatcaa accaaagcca
     6481 aacatttcat tactggtcat tcttatgaca tcggcaacaa tgactttgcc gaggcatcta
     6541 ttgaaaaagg ccagttgatc gttaatcatt tagaagttgg aaaatataat ttagaagaag
     6601 taaaagctcc tgataatgcg gaaatgattg aaaagcaaac aatcacgcct tttgagatcc
     6661 tggcaaatag ccaaacacca gtagaaaaga ccatcaaaaa tgatacgtct aaagttgata
     6721 aaacaacacc tcaattgaat ggaaaagatg tcgcaatcgg tgaaaaaatt caatatgaga
     6781 tttctgtcaa tatcccatta ggtatcgctg ataaagaagg aacgcaaaac aagtacacaa
     6841 cattcaaact tatcgatact catgacgctg ctttaacatt tgataatgat tcttcaggaa
     6901 cgtatgctta tgccttatat gatggaaata aagaaatcga cccagtaaat tattctgtca
     6961 ctgagcaaac agacggattc acggtttcag ttgatccgaa ttatattcct tcattaactc
     7021 ctggcggtac attgaaattc gtttactata tgcatttgaa cgaaaaagca gatccaacca
     7081 aaggattttc taaccaagca aatgtcgata acgggcatac aaatgatcaa acaccaccgt
     7141 cagtcgatgt cgttactggg ggcaaacgat ttgttaaagt agatggtgac gttacatcag
     7201 accaaacact tgctggagca gaattcgtcg ttcgtgatca agatagtgac acagcgaaat
     7261 atttatcgat cgacccatcc acaaaagccg tcagctgggt atcggcgaaa gaatcagcaa
     7321 cggtttttac aaccacaagt aacggtttaa tcgatgtgac aggtctaaaa tatggcacgt
     7381 actatctgga agaaacgaaa gcgccagaaa aatatgttcc attaacaaac cgtgtagcat
     7441 ttactatcga tgaacaatct tatgtaacag caggacagtt gatttctcct gaaaaaatac
     7501 caaataaaca caaaggtaca cttccttcaa caggcggtaa gggaatctat gtgtatatcg
     7561 gtgcaggagt agtccttcta ctgattgctg gactgtactt tgctagacgc aagcacagtc
     7621 agatttagtc tttttgcaag aattggaaaa aggagcagcg gattacgcaa atgctccttt
     7681 ttcttttatt atcaaacagt catcacaagg agaacacaaa tgaaaccaaa gaaaaggaaa
     7741 cttctagatc tgctgatgct tttcttgctt ctctcaggaa tagggatact agcttatcca
     7801 tttgttagcg acgcgctgaa taattatctg gatcagcaga tcatctctca ttatcagcaa
     7861 caagcggtca aagaaaatga agaagtcatg gctaaaatcc aagaaaacat gacaaagaaa
     7921 aaccagcaat tagcaaaaga aggtggaaat ccaggtgcag atccctttac taagaaaaaa
     7981 gaacctgcga aaaaggatag aacctatttt gagaaacata cgatcggtat tttgacgatc
     8041 cctgagatca atgtccattt gccaattttt gatgaaacga atactgtgct gctagaaaaa
     8101 ggtgcctcat tattggaagg aacgtcttat cctacggggg gagaaaatac acatgccgtt
     8161 atttccagcc atcgaggact gcctcaagca aagttgttca cagacttacc gaaactgact
     8221 ttaggtgatt ctttctatat cgaaatcaat ggacggacac tcgcttatca agtcgatcag
     8281 atcaagacgg tagaaccgac tgatacaaaa gatctgagaa tcgaaaaagg gcaggatttc
     8341 gtgacactcc taacttgtac accttatatg gtcaacagtc atcgcttact tgtcagaggg
     8401 caccgggttc cttatcatgc aaaagaagtt caaaaagaag tacagaaagt atccgagaac
     8461 aaacgatttt tcctgtatgg attgttagct gcatgtatac tgattttcct ccttcttata
     8521 tatctttttc gtcaatcgtt caaaataaga aacagaagtt agctattcct catttctata
     8581 atatatctat tcgaatcgtt ccgtaaacaa acaggctgag gtttgaaaaa gaaaacttca
     8641 gcctgttttt atgatgatat caaagagagc ctatcaaaca ctatctttct tattactaaa
     8701 tgaggatctt ttcttaagtt ttccgatatt ctttaagaag gcctttttgc atcaattctt
     8761 gtttgttttt caagttgcgg atattctctt caaaattttg agaatatatc actgagaata
     8821 acgtatttaa tacgtaatcg aaggcaattt gagaggagaa agtcgctact ttagcaaaat
     8881 catattcctc ctgcgatata acaagacaca tatcagcctg tttggccatt tctgaatgct
     8941 gatttccggt aatcagcaag gtaggggcct ctacatgctt caaataggtc attattcgtt
     9001 cataatgatg gatgcgaccg ctgtagctaa taaaaatggc gcaatcctcg ctagtcaaat
     9061 tggctgcatt ccaagaagaa tcactgtatt cttcagccat gatcaaaaac ttattcaact
     9121 tgaccatttt attctggaat ttccttgccg tgatttgaga atcacctttt gcaaaaagaa
     9181 agattcgctg tgccttgttc aatactttac cgatttgatc taaatcttca tgttcaatct
     9241 gcaattgtgc tttttttatt gactggatcg ataaatccgc cattttcttt gcaatttgct
     9301 gagtgctatc ttcacttgaa aaaggaaaat taggatcaat cgtttgattg aactgagcaa
     9361 tcaattgatg aacttcccgc cccagctcga aacggaattc tttaaatccg ctgaatccca
     9421 tttttttgca taaacgaatg atagctgaat gggaagtata cgtagctttt gctaattctt
     9481 caatcgtcat gtagactgca gcttcgatat tcctacggat gtaatcgaca atccgtttct
     9541 ctgtcgtggt aaacaactct tgttttgtta gtttttcttc aataagcatg atctgacctc
     9601 caaattctat cttactgata aacacttggt tccgctacta tctttttgat ttttatcctt
     9661 actttgaaca ttaatttcac caaaccgaat gataacaagt gtttttctga acttttttac
     9721 cagaatagct attataaata ctttctgcgc ttttctagga tatactgaaa acatcaataa
     9781 aaggaggcac cactcatgct tacaatcgct tatcttggca atggaaagag tacaaatcgc
     9841 tatcatttgc ctttttcaag aaaacttacg aacaaaatca atatcaaaaa aattttttcc
     9901 ccatctgaac cagtgtggaa aacattcgaa gacatcgaat atactgatca gctgtctgat
     9961 ctttgggatg atcctgagat ccaattagtt gtcatcacta cacctagtca atttcattat
    10021 gaatatggaa aaatggcact cgaacatggt aagaatgtac tagtagagaa accttttgca
    10081 gaaacgtcaa aggaagcaga agagttattt gcattagcaa aagaaaaagg tctatttatc
    10141 caatgttacc aaaacagacg gttcgattct gacttcttga cagtccaaaa agtaatcgaa
    10201 agcggaaaat taggggatat tttagaagta gaaatgcatt atgattattt cagaccggaa
    10261 gtaccagaaa atacgcatga attttctact gcgaacagct atttatatgg acatgcttgt
    10321 catacagtag atcaagttct ttcttatttc ggcgaaccag atgatatcag ctatgatgta
    10381 cgtcaacttt tagggaaagg acgaatgaat gattacttcg atctggattt tttctatgga
    10441 aatatgaaaa tttcgataaa atccagttat ttccgcatca aagaacgtcc aagtttcgtc
    10501 gtttacggaa aaaaagggat gttcgtgaaa caaacaaaag atcgacaaga agaacatctg
    10561 aaaatgtttt acatgcctga tcatgaagac tttggtatcg atttgcccga acattatggt
    10621 gtattgacct atatagatga acaagggagt tatcatgaag aaaaagtaat ttccgaggtt
    10681 ggtgattaca gccgtgtcta cgaagggatt tacgagacat tagttaatgg cgcagaaaaa
    10741 gtcatcaaag atgaagaaac aatcttacaa atgaaaattt tggaagaagg aatcaaaagc
    10801 attcaagaat cagaaggaaa aatatgacat atttgtaaga atcgggtttg ttttttgacg
    10861 cataaactgt cttattgtac gatgaaaaag tatagaacta ctaggaggaa ccaacatgac
    10921 aaattatgac ggtgaaaaac acaaacgatt aagaggttca atggccccta aaacagaaga
    10981 agaagtggct ttagcaggaa ctaattctgg tgaaacagaa gacaaagaag aatcattagc
    11041 tggcacaaac tctggtgaaa cagaaaataa agaaacagct ttatctggtt caaattctgt
    11101 agaagaggaa acaaagccta atgagcccaa tcactttgaa aaagatatcg aaaaagcggt
    11161 tgaagatgtc aaagaaaaat ttgaagaact gacctcaaaa aataaacatg atcatcattg
    11221 atcggacgaa atcaattcat aaaagcagga gacatttggt cacctgcttt ttttctgtat
    11281 ccattcgata gaaatcgttc ggtacagtcg taaaaaaaat ccaactgtaa gttgtaccaa
    11341 acacccctcg atttaaaagc gctaccatac tagaatataa gtaggaggtg aacagcgctg
    11401 tgaactacaa aattagttat ctcctgaaaa caccaagaga aatcagtcta gaaaaagcag
    11461 cagagatcat cgaagtaaat acaagcgagg cgatcaaaca attaactcct tttgccgaat
    11521 atgtggatga tacccgtatc cgtataaaga aattccgagc aatcgactgg gcagaaaaat
    11581 ttttggatat tactcgattt gaagctgtgt ataccgaaca agagcgaaga gatcttcttt
    11641 atttgatggt ttattcgact gcaacgtctg tttctgtttt tcattttcaa gattttcttc
    11701 aggtttctaa agggacagtc ctgtcagata tcaaaaagat aagaaatgaa ttagaagtgg
    11761 aagattgtca gctgtcgtat actaggaaaa ctggttttag gctgctaggg tcagaagcaa
    11821 tattaagaag agaagcaaaa aatcgcactg ctcgtttgat gcagtcactt gctggaagat
    11881 ttgggctcac gtatttaacc gcaacccttt ccattgatta ttacgccaaa gttagagatt
    11941 tgctagataa aacgatttca ctatcagatc ttcgattcgc accaaatcga atggatgaat
    12001 tagcttatta tttgagtttg acaaccattc gaattggaaa aacatccatg gaactggtaa
    12061 aagatgctga actgttggct tctcttgcag tcactgacat tagtcgacaa gtattgactg
    12121 gccttggcta ttccgaacat caaaattcgg agattatatt tttctctctt tgcttagcat
    12181 cgatcgttga aggagatgtc caggagcctg cacttgattt tttgctggaa tgttcttcag
    12241 aagtcatcca tcgcatggaa ttcctgatgg ccgtagaatt tgcttcgttt cgtgaattat
    12301 tgatgaatgt cttttatcac ttggtccctg cctattttcg tatttcctat tctttttatt
    12361 tacctaatgt gatgatcgat caaattaagc atcaatatgc ttccatctac gaaatgacaa
    12421 gaaaagcatt acttccgtta gaaagacgaa tagggaagag catccctgaa gaagagattg
    12481 gttttttcac tatactattt ggcggagaga tcagaaaagc ggacgcagaa gaaagaaata
    12541 gaaaaataag agccgtgatc gtctgtccaa gtggcattag ctcttcattg atcttaaaaa
    12601 gtgaacttca gcaattattc ccgatgatct tttttacaga gactaattca gctgaccgct
    12661 tacacgaggt tgacgaaact tcttacgata ttattttttc tactgtacct ctcacactta
    12721 ctgataagaa aaagtctctc tatgttctgc atccaatcat gactgacttg gaaaaaaatc
    12781 agctgatcaa tcaagtacaa agagattggc tgataccagg attttcaatg ccttctgccc
    12841 aagaattgtt gtcggcgcta ctgccttata tcgaattaaa agaaggcgta gacgaagaaa
    12901 agctctatca agtattaaat cgtaaaatga acaaactttt agaaaagaag gaggatcaac
    12961 gtcctatgct atctgaatta ttaacagaag aaatgatcca attatcggat caaccaaaaa
    13021 actggcaaga agcaatcaca tttgccgcac agccgctatt atcagaaaat aaaatagaag
    13081 aaagttatat caaagctatg atcgagcgcg tggagcagta tggtgcgttt atccatattg
    13141 gcgatcacat cgcattacct catgctcgtc cagaagatgg cgtaaacgaa gtagggatgt
    13201 ctttgctgaa acttcaacaa cctgtggatc tgcttgataa tccagcccat cccattagtt
    13261 tgttcatctg tttagctgct atcgataatg aggcacatct tcgggctttg gcaaatttaa
    13321 caaagctact ttctaataaa caaaatcttc aagatttatt acacgcatca acaaaagaag
    13381 aaatattagc gatcataaga aaaggagacg aatgatatga aaatttttgc agtatgtcaa
    13441 agtggattag gaacaagttt tatggtagag atgaacatca aacaagcact tgcagatctt
    13501 ggagcagatg tggacgagtt tgaagtaagt catacggatg ttggaagtgt atctagcgat
    13561 atggctgatt atttcttcat tgagcattct ttgaaagata cgctaacttc tatacctgat
    13621 gaaaagctcg tacctttgca atcgatcatt gacagcgaag aagtaaaaga aaaagtaacg
    13681 aagatcttgt caaaagaata aaaatgattc aagggaggaa ctatcaatgg ataaattgtt
    13741 gaatttgttt gtgtcaatcg tcagtcagcc ctccatcctc gtttctttga ttgcactgct
    13801 tggtctcgtt cttcagaaga agaaattttc agaagtgatc caaggaacta tcaaaacatt
    13861 tgtcggattc ctagtactga tcggcggtgc tggtttaatc agtaattcac taaccccttt
    13921 tgcttcgatg ttccaaacag ctctgcatac tcaaggagta gttcctagca atgaagcagt
    13981 agtggcgatt gctttgcaac aatacggtac acctactgct ttgatcatgc tagtagggat
    14041 gatcgtgaat attttacttg ctagattcac aagatataaa ttcatttttc ttacaggtca
    14101 agcaatgctt tatgtttctt gtctaacagc agtcatttta gtaagcgcgg ggtttactgc
    14161 aaatttgtgg atgatcttat taggcggggt cttcgaagga acactattaa ctattacgcc
    14221 tgctctttgt caaccgttta tgaagaacat cactggaaac aatcaagttg cgttagggca
    14281 taccggaaat atcggttatg cttctagtgg atggatcgga aagttttttg gaaacaaaga
    14341 ccgttctaca gaggacataa agattcctaa aagtttcagc tttttgcgag attctactgt
    14401 atcgatcatg ctcttgatgt cgatcgtata tgtcgcactt gctttgttag ctggtcctgg
    14461 atttgttgaa agagaactaa gcaatggaga caacgctgta atctttgcac tgatccaagc
    14521 tggaacattc acagctgggt ttgttatcgt ccttcaaggg gttcgaatgg tgttggcaga
    14581 aatcgttcca gcattccaag ggattgctaa aaaactcgta cccgattcta aaccagcatt
    14641 agatgttcct atcgtttttc catatgcacc taatgctgta ttaattggct tttttgttag
    14701 cttctttgta ggaacactat ccatgtttgc tatgataggc ttgcaaacta cagttatcat
    14761 tcctggggta gtaggacatt tcttctgcgg agctgcttct ggtgtcttcg gcaactcgac
    14821 tggcggtaga agaggggcgg tattaggttc tgctttcaat agcttattga ttagttggct
    14881 gccattattt atccttccag tattaggaga tcttaaaatc gcttcttcta catttgccga
    14941 tactgattat ctagtaccag gacttgtact tgggaaacta ggagaatacg gtcaaatagc
    15001 cttgacagtt ggaatcatcg ctttcttttt acttgtcgta ttatctagct ttctttttgc
    15061 cggaaaaaga acaaacattg tttctaaaga acagaattaa gcaaacttca aaataaaaat
    15121 tgcttggaca atatcagaag aagatttaaa gtcttctgat atgtcgcaag cagttttttg
    15181 ttttatgcat ttatttttga atagcttgtt aagacaagtg gctgttttgc tagaagagga
    15241 taacttttac cattcatttt cactgttccg cctgttctat cagaagtaat cacgatctta
    15301 tcttttgttt tttcaagcga aatctttcca tctttgatag gtagcgtaca attcagtgtt
    15361 tcaaatgaat ctagattagg ttgtatcata aattcttcat aacctggctt tgttggatac
    15421 aaaccgataa aatagcgtcc taaaagatag attgggcttg ctccccatgc atggcataag
    15481 ctttttccat aaggatctcc atacatactg tacttttcat ttcctgtttg agtagggtcg
    15541 tattcttccc agaaagttac cgcatcatga gataacatcc ctccccaata atcaagaatc
    15601 gtttggtaga cgatattcgt ctgtccgagt ttgcataaag catcttgttc atagaatttg
    15661 aaatatggag tagtaatctg tgtgatagtg tcattcaata agacattctt taagatcgat
    15721 tgctgttggt ctttgttagc aaaatcgaat aaaacagcga aaatattcgc atgacgcgtc
    15781 acatttcttc tcccagattc atagcaatcg ataaaagcgc caagttcttc atcccaaaag
    15841 tagtcaaata ggttttgttt caatagttga tatttttctt gatagagatg agtatgataa
    15901 ttttcgttct ctccatatgt ttcagctaag atatctccgc aagtgatcat ggaatgatag
    15961 gctttcagta ataagacttg ttctgcagca agcgttcctt ctttatccat ttcagaccaa
    16021 tcaataaata tccaatcttt tgctctccca taaataaacc ctaattcatt cgtttgctcc
    16081 atcaagtatc tcattaatga ttgcatctta gggtagacga tgcgtaaaaa ttcatagtct
    16141 ttcgtcatca tatagtggtt ttcgatacca atcaaccaca agatcgaata gtcaacgata
    16201 gtattcatat gctgcttgat ttcattttgt cctcttaatg ctaatatcgt tcgtttattg
    16261 atttcctcgt caaaaaaaag atattgattg atcatgaatg attgatacgc atctccagac
    16321 caaatccagc gatctcgttt gataccgtcg ataaaaaaca aatcactaca taacttgaag
    16381 gcttcttctg atactgacca gatctgattc agtaaagaag aatcgcttga aaaagcagct
    16441 tgtttcgaaa gagataaaaa ttcatgaaaa acttgaagtt gaaccattcc agtagaaacc
    16501 tctggtataa acaaatagcg aaaagcgcgt ttacgagctt tcatttttct tgtcattcct
    16561 atttgcttat agtagcaata atcgatatcc atagcttcta catcggactc tccataacat
    16621 agagtgatag attcgatatg gtcatccaga gatattagtt ctacacagcc attcactgct
    16681 cgcccaaaat cgaataagat tcctccgcca atctccgttt cagtgactgg atggacgcat
    16741 tctttttgat aatagatttg atttggatct tttgcgatat cgacataaag cggactccag
    16801 ccagctggat attcttcaat aaaattactt gctgtccagc caatatctga ttttacttcg
    16861 tctccctcga taaacatcgc tggtagccct gatgtatgtc ctacaaagat acgaattttt
    16921 gctttaccag ctggacaagt cagccatttt ccacatggat gtttttgtcc attaatttct
    16981 acatacccaa ctccttggat cgtcacttta aacatggaag gttgatcaag aaagtaatat
    17041 ctcttaaact tgacgttccg atggcaatca tccatgtacc agtacgctgg ccagtcaaaa
    17101 cctcgttctt cccgttggaa attttgtagc agtccatggc ggatctcaaa gtctccagga
    17161 taccaaagcc atctatcttt atgtttttca tttgtcattt tacacgacct ccatcaaatt
    17221 tattttgttg tcaattgttc ttttatttct tcatctactc ttatcactgt ctttctattc
    17281 gtcctgtgta ctactagaag gctaataagc gcgctaatca acagcaagat actgccattg
    17341 acgaaaacag caagtgtttt actcccaaga gaagcaaaag agagaaagag caaaccatat
    17401 ggtgcaatga aagtacctaa attagcgcca accaataaga ttgaagtatt gaaactctcc
    17461 gcgtacttgg gcgcttggat acttacttgg ttgaataagt aaggaatgaa taacgaaaaa
    17521 gaaattcctg ttaggatcgc accaaaccca atcacataca ggtttatcac agtggcaatc
    17581 aatacaaagc cgattcccat cagtagtaaa gaaagtgtaa gcatatagcg atgtgctttt
    17641 gtctgaagcc agccaaaaca aactccggca gccatacttg ccaattgcat gattgttaag
    17701 atattcgctg cattagtttc gtttcctaga gaataagtcg tcaggactgt ggctaattta
    17761 atcgatacag cattatatac cacgatgacc agcaagagaa aaataccata actaatcatt
    17821 ttaggattcg ttttgattac ttgtcttgtt ggggctgtgc catgcgattg aagttcagtt
    17881 gtttcaggtg taggaacaaa caaaacaaac aagacaagaa taggtaagct gatggcgtag
    17941 actaaaaagg tatattgcca accgaactgt actagctgtc ctgcaacaaa tgtcattaaa
    18001 gctgcaccaa atccttgaaa tgcactttga aaaccaatca tagaggctcg ttcatctccg
    18061 tgataaaagg cagcaatcat gctgactgcc aaagaattga tcaatccaaa gccaatacct
    18121 agtccagctc ttgaagctaa gacaatactg taactttgtg agaagagggg gaggatacca
    18181 caaatcgatg caatcactaa tccgatgatc acagtttgtt tttgtccgat tttcctagca
    18241 ataaaactac taatcaaaac aaaaatagtg accataaaag agggagcagt cgttaataat
    18301 tcaacagcag ataaagaacg cgtggcataa gtagctgaca tactgggaat tgcagacgtg
    18361 actgcaccag cagaagtcaa aataagagag actgacagca gagaaaattt catcatcttt
    18421 ttattcataa gcatgttcct ttcatttttg taagcgctta tcaaaaatat agtatgaagt
    18481 ggttattctc tcaatagcgg aatcacagga agttcatgtc ccaaccatag aaagtcttta
    18541 tgaaaccaaa aatagctttg catataatag cagtacagcg tgaactaata agcgaaagaa
    18601 accgcttaca aaaaggaggg agtcaattgg aactagctga attagacatt ttttttgcgt
    18661 aacgaagaat caatcgaaac tctgcagcgt gaacaaggaa tgaatatcaa tgatttaaac
    18721 ttagcttacc atcttcagac accgccaaaa atccaccgag gggatctatt tcaacaggga
    18781 aatattcata tcagtaaaca ccgtcgatat gcggaagttc ccgagcatac acatgatttt
    18841 gttgaaatca attatatgta tagcggtaca tgtttacaaa ttattaatgg agaaaggata
    18901 gagatggaag aaagtgacgt ggtgatcatt gataaagaag ctactcaaag aatcgaatac
    18961 acagggcaag aggatatttt aatcaacatc ttattaaaag aagaaacctt atctacagag
    19021 atattgaatc atttggcaag cacaaataat ctactttctt cttttttgat aaatgcatca
    19081 aaagaaaaag gggaacatac acaattcatt cattttcgcg gcggaaaaac aagtcgtttg
    19141 acgcatttga ttgaaaatat gctgttgatt tattttgata catacagtca taagatccgt
    19201 tcgttaaatc tctatacatc actattactg atcgaattga ctcatttatt agaacaacaa
    19261 agcttttcat cagcagatgg atgcgcagaa aatgagatgc ttagagtatt agattatatc
    19321 gataagcatt ttacttcttg tacgttaaaa gaaacagcag ctcattttgg ctacaatccg
    19381 gtctatctta gtaataaatt gaaaatgtat tttgatgctt cctttcaaga actggttcta
    19441 aagaaaaaat tcgactttgc gttggaactg attcaagaaa cgagtttttc gttggaagaa
    19501 atcgctaatc gtatcggata taaacaaacg acttctttat tcaaattatt caagaagtat
    19561 tcaacatgta ctcccaatga atggagaaat aaacattaag aagaaaatct ttcactaaag
    19621 catatgaagg aaaattgttt taggtgcgat tgctgtctta ttatatcgtt gcagcgaatt
    19681 tttttggatt tattttcagt tctaaacttt tggttgtatt atcaggggat ataggtgtaa
    19741 acatttgtca tttatctgtt atgcgtattc aggaattcgt ttccatcaaa aattataata
    19801 acaactatta taattaacat gttgataaaa tgaataaaca agactatact tttcatgaaa
    19861 gtaacaaata gttataaaaa ggagctgttt attcatgaaa aaaacaagtc gtaaagtagt
    19921 aatcgtcggt acaggttttg ttggaacaag tatcgcttat gcaatgatca accaaggaat
    19981 ctctaatgaa ttagtattga tcgatgtgaa tcaagaaaaa gcagaagggg aagcattgga
    20041 ccttttagat ggtatggctt ggggcgatga aaatgtggct gtatggtcag gtggctatga
    20101 agaatgtaaa gatgctgata tcgttgttat cacagctggt atcaaccaaa aacctggtca
    20161 atctcgttta gatttagtta aaacaaatgc gtcaatcatg cgccaaatcg taaaagaaat
    20221 catgggatca ggattcgacg gtattatcgt tgttgcttca aacccagtag atattttgac
    20281 atatatcgct tggaacgaat ctggtctgcc aacatcacgt gttatcggta ctggaacaac
    20341 attggataca actcgtttcc gtaaagaaat cgcattgaaa ttaaaagttg acccacgcag
    20401 cgtccacggc tatatcttag gtgaacatgg tgattcagaa gttgctgcat ggtcacatac
    20461 aactgtcggt ggtaaaccag tgtttgaaat tgtagaaaaa gaccatcgta ttgcccaaga
    20521 tgaattagat gtgattgctg ataaagtccg taatgctgct tatgagatca ttgatcgtaa
    20581 aaaagcaact tactatggta ttggtatgag tactgcacgt atcgtaaaag ctatcttgaa
    20641 taacgaacaa gctgtattgc ctgtatctgc ttatttaact ggtgaatacg acgaaaaaga
    20701 tatctttaca ggtgtgccat caatcgttga cgaaaatggt gttcgtgaag ttgttgaatt
    20761 atcaatcaac gaagaagaaa aagcaatgtt cagcaaatca acaagtgctt taagagaagt
    20821 attgaacact gtattataag aaacaatgaa tatgaactaa aaatcctaaa actgcttaac
    20881 agctgaaata gctgttaagc agttttttat tgatatctaa taggtaagca aataaacaga
    20941 atggttaaaa ttaatcggtc cgaaaatgta tccgtacata tgaacatatg aaatttaatc
    21001 tgttgattac aataagcgaa tcgttatcaa tgtttatctg tggttaatta aaaaatcctt
    21061 ccgcaacggc aaatacaatg attcatatct aattcgttca tatttgatta actgaataaa
    21121 gaaactggtt tttatcattc aattaaacga caaaaaaagt aagaaaaact gcaaaatttt
    21181 tttataattt tgcataagca gattttgatg gaaaaatgac gatttttgat aaaaaaccgt
    21241 tcgagagagg tttatcattt tttaaatcta ctttgtataa tatatattat gtaaactaaa
    21301 atatataaaa aatc
//
LOCUS       DACDMM010000033        21023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDMM010000033 DACDMM010000000
VERSION     DACDMM010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.033"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWD16_000947"
     CDS             complement(<1..132)
                     /locus_tag="IWD16_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9088029.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..1090
                     /gene="hslV"
                     /locus_tag="IWD16_000948"
     CDS             542..1090
                     /gene="hslV"
                     /locus_tag="IWD16_000948"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP9088030.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            1104..2504
                     /gene="hslU"
                     /locus_tag="IWD16_000949"
     CDS             1104..2504
                     /gene="hslU"
                     /locus_tag="IWD16_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP9088031.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            2522..3313
                     /gene="codY"
                     /locus_tag="IWD16_000950"
     CDS             2522..3313
                     /gene="codY"
                     /locus_tag="IWD16_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP9088032.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            3395..4270
                     /locus_tag="IWD16_000951"
     CDS             3395..4270
                     /locus_tag="IWD16_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9088033.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(4402..5040)
                     /gene="plsY"
                     /locus_tag="IWD16_000952"
     CDS             complement(4402..5040)
                     /gene="plsY"
                     /locus_tag="IWD16_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP9088034.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            5167..5601
                     /locus_tag="IWD16_000953"
     CDS             5167..5601
                     /locus_tag="IWD16_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP9088035.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5767..7812
                     /gene="parE"
                     /locus_tag="IWD16_000954"
     CDS             5767..7812
                     /gene="parE"
                     /locus_tag="IWD16_000954"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP9088036.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7824..10274
                     /gene="parC"
                     /locus_tag="IWD16_000955"
     CDS             7824..10274
                     /gene="parC"
                     /locus_tag="IWD16_000955"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP9088037.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10551..12782
                     /gene="pflB"
                     /locus_tag="IWD16_000956"
     CDS             10551..12782
                     /gene="pflB"
                     /locus_tag="IWD16_000956"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP9088038.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            13026..13787
                     /gene="pflA"
                     /locus_tag="IWD16_000957"
     CDS             13026..13787
                     /gene="pflA"
                     /locus_tag="IWD16_000957"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP9088039.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13872..14798
                     /locus_tag="IWD16_000958"
     CDS             13872..14798
                     /locus_tag="IWD16_000958"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP9088040.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14980..15873
                     /locus_tag="IWD16_000959"
     CDS             14980..15873
                     /locus_tag="IWD16_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP9088041.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            16007..16132
                     /locus_tag="IWD16_000960"
     CDS             16007..16132
                     /locus_tag="IWD16_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9088042.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(16266..16898)
                     /locus_tag="IWD16_000961"
     CDS             complement(16266..16898)
                     /locus_tag="IWD16_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088043.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(17046..17588)
                     /locus_tag="IWD16_000962"
     CDS             complement(17046..17588)
                     /locus_tag="IWD16_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP9088044.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17723..18112
                     /locus_tag="IWD16_000963"
     CDS             17723..18112
                     /locus_tag="IWD16_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088045.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            18116..18880
                     /locus_tag="IWD16_000964"
     CDS             18116..18880
                     /locus_tag="IWD16_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9088046.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            19009..19956
                     /locus_tag="IWD16_000965"
     CDS             19009..19956
                     /locus_tag="IWD16_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9088047.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            20086..20826
                     /locus_tag="IWD16_000966"
     CDS             20086..20826
                     /locus_tag="IWD16_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088048.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7389 a         3577 c         4414 g         5643 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaaacaaa
      541 catggtcgaa tcaccatttc attcaaccac tatctgtgcc gtcgaaaaag acgggaaatt
      601 tgctatggct ggagatggcc aagtaacaat gggtgaatct gttgtcatga aaggcactgc
      661 aaagaaagta cgacgtatct acaatggaga agttgttgta ggtttcgccg gaagtgttgc
      721 agatgcgttt actttagaag aaaaatttga aggcaaacta aacgaatata acgggaatct
      781 acaacgcgca gctgtcgaat tagctcaaga atggcggaca cagcaatcta tgcaaaaact
      841 tgaagcgatg ctgatcgtaa tgaatgataa agaaatgctt cttgtttcag gtacaggaga
      901 agttatcaca ccagatgatg gtatcttagc tatcggatca ggagggaatt ttgcgttggc
      961 tgcagcccga gcaatgaaaa actatgctca aaacgacatg agtgcgaaag aaattgcaga
     1021 aaatgcgtta aatattgccg cagatatctg tatctttact aatcataata ttatcgtaga
     1081 agaactgtaa gaggtgagta gatatgtatg aattaaaaca aacaccaaga caaatcgtaa
     1141 gtgaattaga tgaatatatc gtaggacagc acgcagcaaa aaaatctgtt gccgttgctt
     1201 tgcgtaaccg ctatcgccgt ttgcagttag atgaaaaaat gcaacaagat attacaccaa
     1261 aaaatatgct gatgatcgga cctacagggg tcggtaaaac agaaatcgca cgacgtttgg
     1321 caaaaatcgt caatgcacca tttgtcaaag tcgaagcaac aaaatttact gaagtcggtt
     1381 atgtcggaag agatgtagaa tcaatggttc gggatttagt agaaaatgcg atccaaatcg
     1441 tagaaaaaga gcagtacagc cgtgtataca caagtgctaa aaaaaatgca gaaaaacgtt
     1501 tggtaaaaat cttggtacct ggtatcaaaa aagaacagaa acaatccact aatcaatttg
     1561 aacaaatgat gaatcttttt aatgctagtc agcaaaatac cgctgaaccg caagaagaag
     1621 tgacagaaga tattcgaatc aatcgccgta cagtcttgga acaactacaa aagggtttac
     1681 tggatagtcg tgaagtaacc attgaagttg aagaccaaaa gaaacctgca ccaatgatga
     1741 acaatggatt ggagcaaatg gggatcgacc taaatgaaac tctaggtgct ttgacaccga
     1801 agaaaaaagt ggaacggacg gtaacagtca aagaagcttt agagttattg atcaaggaag
     1861 aatcagcaaa attagtcaag gatgctgata tccatagtga ggcaatcaaa ttagcggaag
     1921 aaagtggaat tatttttatc gatgaaatcg ataagatcac ttctaaatca cagcaatctg
     1981 gagaagtatc cagagaaggc gtccaaagag acatattgcc aattgtcgaa ggttcacaag
     2041 taaacacaaa atacgggatc atccaaacag atcacgtcct attcattgct tcaggagctt
     2101 tccatttgtc taaaccaagt gatctaattc cagaacttca aggacgcttc ccgatacggg
     2161 tagaattaga tgatttgaca gctgaagatt ttgtgaagat tctgaccgaa ccaaataatg
     2221 ctttagtaaa acaatacgtc gcgttgatcg gtacagaaaa tgtgtcagtt acgtttacaa
     2281 aagaagcaat tgaacggatc gctcaaatcg ctttcgatgt caaccgtgac acagataata
     2341 tcggcgcacg cagactgcat accattttgg aaaaactact agaagatcta ttatttgaag
     2401 caccagatat gcaaatggga gaaataacga tcacagaagc atatgtggat gacaaacttg
     2461 gctcaattgc ggcaaatgaa gatttaagtc gctatatttt ataaaaaatc ggaggacgac
     2521 aatgacagac ttgctaacga aaacccgcaa gataaacaca cttttgcagc aaaagaatac
     2581 ttttgacatg caagcagatt tgccttacga taaaatggct gtcacactag gtggtattct
     2641 tgacagcaac acctatatca taggaagtga cggagtatta ctaggttata atgaacgtca
     2701 cgatgtgaac aatgaacgaa tcaaaacaat gtttgttgag aaacaatttc ctgaatctta
     2761 tactgataca gtggcacagt tgatgaaaac tgaagctaat atcccaatta ctagtgatat
     2821 tactgcattt ccggtagagt caagagagaa atatccattt ggtctgacga ctgtcgttcc
     2881 gatctttgga gctggcgaac gtttgggtac gattatctta tctcgtatgg aacaatcgtt
     2941 tgcagatgac gatctagtct tagcagaata tggagctacg gttgtcggta tgcagatcct
     3001 ttatcagaag tctcgaagca tcgaagcaga tgtacggagt gcaacggctg tccaaatggc
     3061 aatcaacaca ttatcttata gcgaattgaa ggctgtacaa gctatctttg aagcattgga
     3121 tggagatgaa ggtcgtctga ctgcttctaa tattgccgat agtatcggca tcactcgttc
     3181 tgtgatcgtc aatgctttac gaaaattgga atcagctgga atcattgaat ctcgttcgtt
     3241 aggcatgaag ggaacttatt taaaagtatt gaattcccgt tttaaagacg agttagcaaa
     3301 acacagttat tagaagtaag gggactgggg cagatagaaa tgtttgttcc agccttccca
     3361 ttttaaaaag taagtcgaag ttggaggaat ggcaatgact gttattattc aaaatgacca
     3421 gctgatcgcc gaaatcgctg aacacggtgc ggaattgatc agtctgaaat caaaagaaca
     3481 tggtttagag tatatctggc aaggtgatcc agcgtattgg ggcagacatg caccggtttt
     3541 attccctttt gttggacgtt taaaagatga tcagtatgtt tatcaaggga aaacatatca
     3601 aatggggcaa cacggatttg ctcgggatat ggattttgaa gtcattgaac atggacaaga
     3661 aatggctagt ttcatcttaa ggagtacgcc ggagacatta gaaaagtatc catttgattt
     3721 tgaattagtg atcagctatg aattaggagg agacggcatc acggttcatt atcaagtggc
     3781 aaataccggt gaaaaagaaa tgttcttttc tatcggtgga catcctgctt tcaatgtacc
     3841 tcttgaagaa ggattgtcat ttgaagattt ctatctggca ttttctccat tgaaatcaag
     3901 attacgccta ccattgaaag gcccattcat cgatatggat caaaagacgt taggtcagac
     3961 aaatacgaat ctggctttga atcgtgaact ttttgagcaa gatgccttgg tttttgaaac
     4021 aaggggtcta aatgcgtacg ccattcgaag tgaaaaatct tcccatagcg taactttaag
     4081 ctataaagat atgccttatg tagggatctg gtctccttat ccaaaagaag cgcctttcgt
     4141 ttgtatcgaa ccgtggtgcg gaatcgctga cacggtgaac agtagtggaa acttgatgga
     4201 aaaagtaggg atcaatcaac taggtgccca tgaaatcttc aagacaaaat attccatcac
     4261 agtgaagtaa gaggttgtga ccgagaagtt agcagggaac accgtttacc aaggtgtgta
     4321 aaaaagctat cctgtaagta ataagggagt gtgacatcga tttgtcacac tcccgtttta
     4381 tctctttgga atgaaaaagt atcaggattt tttcttgctg ctattcaaac caaacgggat
     4441 tcgactttca gtatggtttc tgatacgctt gatattgtct ttatgccgat aaaagataaa
     4501 ggaagaaact aaaaaggcaa tgatcgtcag taaccaattg ggcttagcca aaatcacagg
     4561 agctacataa ggaaggacga tcgtagataa agtgatcaat actgcactga tcatactgct
     4621 caaactcacc atacttgtac aaaataaact aacgataaag atgatacaag aataaacaaa
     4681 aaatacagga ttaaatccta aaagcattcc tgcacttgtc gcaacagctt ttcctccttt
     4741 gaacccagca aaaatcggaa atgtatggcc aagaatggcg cataccccaa accaaagagg
     4801 gttaatccca gaaaggtgga agatcatcgg caaacaagta gccaatgtac cttttaaaat
     4861 atccataagc aggacaacaa tcccggctgt tttgcctaat acacgaaatg tattggtcgt
     4921 tccagtatta ccgctcccgt gttgtcggat atcttttttg aaaaacaatt tgccaatcca
     4981 gacaccggaa ggaatagaac ctaaaagata tgctaaaata agtaataaga tcaatttcat
     5041 tttttaccct ctttcaagtt gaaaactatc ctttatttta gcatgaatag gtaacaggaa
     5101 caattgaaaa aatgaaagat acaagtataa tagatggaat aaaaagaaaa gcaaggggtg
     5161 gcagaaatgt ctgtaaaaaa tccaacacaa gaaacgattt tcaactattt ggaagaagca
     5221 aaaaatattg ccgtcgtagg cttgagcaat aaaccagaac gaacaagtta catggttgca
     5281 caggtattgc aagacaatgg atataaaatc ataccagtaa atccattatt ggctggtgaa
     5341 gaaattctag gcgagaaagt ctatgcgaat ttgacagaaa tcgatgatcc aatcgatatc
     5401 gtagatgtat ttagacggag tgaattttta cctgaaatag caggtgattt tttgaaaact
     5461 gatgctaaag tgttctgggc acaattaggg attgaaaaca aagaagcagc tgagatgcta
     5521 agaaaagcag gacgtacaga tatcgtaatg gatcgttgta ttaagattga acttggtaag
     5581 ttgaaagaaa accaaaaata aaaatgacca caaagaagag atgatcgata gaggggaatg
     5641 tcgaaaaagc ggttaggaga taattaaaat tgatttctcg tctaccctct tttttttcac
     5701 gaccttttct agtatgataa tcgtggtgcg gttttgccgc aaagtattat tgaaggagcg
     5761 tttgccttgg caaaaaaagt aaacaacgaa tacaatgact cgtccatcca agtattggaa
     5821 ggattagagg cagttagaaa aagaccaggg atgtacattg gatccactga cagccgaggc
     5881 ttgcatcatt tagtctatga aatcgtcgat aatgcagttg acgaagcatt atcaggttat
     5941 ggcagtgaga ttgatgtgac catccatgaa gacaatagta taactgttgc agatagcgga
     6001 cgtgggatgc ctgtaggaat gcatgcttcc ggaataccaa cagttgaagt gatttttact
     6061 gtgctgcatg ctggcggaaa gtttggtcaa ggagggtaca aaacttctgg tggactgcac
     6121 ggtgtaggag ccagtgtggt aaatgcctta tcgaaatggc taattgtaac gatcgttaga
     6181 gatggcgtag agtatcagca gaaatttaaa aacggcggta aacctgacgg tactttgaaa
     6241 aagataggaa aaacaaaaaa agcaaacggg acgactgtcc attttttacc agatgataca
     6301 atattcagta caacgaaatt ttcatatgaa attttagccg aacggttaag agaatctgcc
     6361 tttcttctaa aaggcgttaa aatctctctt tctgatcttc gtggagaaga accagtcaag
     6421 gaaatttttc attacgaaga aggaatcaaa gaatttgttg attatctgaa tgaagaaaaa
     6481 gatacattga caccagttgt ttatttttca ggagaaaaag aaggtatcga agtagaggtc
     6541 gcttatcaat acaatgacgg ttattcagaa aatgttttat cttttgtcaa taacgttcgg
     6601 acaaaagatg ggggaaccca tgaagctggt atgaaagcgg cgatgacgaa atcttataat
     6661 gaatatgcga gaaaagttgg tttgctgaaa gaacgggaca agaatctgga aggtagtgat
     6721 ttcagagaag ggttagctgc tgtactttct atccgtgtac ctgaaaatct tctgcaattt
     6781 gaagggcaaa caaaagaaaa acttggtacg ccagttgctc gtaccgtagt agataacgtc
     6841 atcagtgaac aaatgggctt ttatttgcag gaaaatagcg aaatgagcca gatgttggtc
     6901 cgtaaagcaa tcaaagccag agaagcgaga gaagcagcac gtaaagccag agaagaaagc
     6961 cgaaatggga agaagcgtaa aaaaggcgaa tctcttcttt caggaaaatt gacacccgca
     7021 caatcccgaa accctaagaa aaatgaattg tatttagtcg aaggggattc tgctggcgga
     7081 tctgctaaac aaggaagaga cagaaagttc caagcaattc tacctttacg aggaaaagtc
     7141 atcaataccg aaaaagctaa aatgcaagat atcttgaaaa atgaagaaat caatacgatg
     7201 atctatacga ttggtgcagg agtcggtcct gagttttcta tcgaagactg taattacgac
     7261 aaagtaatca tcatgaccga tgcggataca gacggcgccc atattcaagt attactgctt
     7321 actttctttt atcgttatat gaaaccgctg attgaagctg ggaaagtata tatcgcgtta
     7381 ccgcctttat ataaagtatc caaaggacaa ggaaagaagc aagtcattga atatgcgtgg
     7441 acagatgacg aattggcagc aatgattaaa aaagtcggca aaggatatat gctgcagcga
     7501 tataaaggac ttggagagat gaatgcggaa caattgtggg aaacaacgat ggatccgact
     7561 tcccgtacgt tgatccgcgt ccgtatcgat gatgctgcgc aagctgaacg tcgagtaacc
     7621 acactgatgg gtgacaaagt agaacctcga agaaaatgga tcgaaaatca cgtgcaattt
     7681 acgctcgaag aagatggcag tatcttagat aaaaaagagg atactgaaat ctctccttca
     7741 gtttctaatg atttattaga tgaagaacga gcagataaaa atgaaaacaa tcagttattt
     7801 gaagtagaat aggaggtcac ctgatggaaa atcgacaaga agtacaagaa ttaactttag
     7861 aagaagtgat gggtgaccgc ttcggacgtt attccaaata tattatccaa gaacgtgcac
     7921 tgcctgatat ccgtgatgga ttgaagccag tgcaacgccg gatattattt gccatgaaca
     7981 aagatggcaa tacgtatgac aaaggattcc gtaaatcagc aaaatctgtc ggaaatatca
     8041 tggggaatta tcatcctcat ggtgacatca gtatttatga agcgatggtc cgcatgagcc
     8101 aagactggaa gttgcgggaa gtactgatcg aaatgcacgg gaataacggg agtatggacg
     8161 gtgatccacc tgctgcgatg cgttatactg aagccagatt atccaagtta agtggagaga
     8221 tgctagcaga tatcgagaaa gaaacagtcg atctcgtgtg gaattttgat gatacagaaa
     8281 aagaaccaac agtccttcca gctcgatacc caaaccttct cgtcaatgga tcgacaggta
     8341 tatctgctgg atatgcaaca gaaatcccga ctcataactt agctgaagtg atcgatggca
     8401 caatctatat gatcgatcat ccacaagcga gccttgaaaa attgatggaa tatattcctg
     8461 gacctgactt tccaacagga gggatcttgc aaggaaaaga tgaaatcaaa aaagcttatg
     8521 aaacaggacg aggaaaagtt attcttcgtt cgaaaacaaa gatagaggca atgaagggta
     8581 ataaacagca aatcgtgatc acagaaatcc catatgaagt aaataaagcg accttggtga
     8641 aaaaaatgga tgagatccgt ttgaataaga aaatcgatgg aatagctgaa gtacgagatg
     8701 aaagtgatag aacaggtctg caaattgtcg tcgaattgaa aaaagacgcc aatgctcaag
     8761 gaattttgaa ttatttgttc aaaaacactg agctacagat caattacaat ttcaatatgg
     8821 tcgctatcga ccatatgact ccgcatcaag ttggactgaa agacatttta agaagttata
     8881 tcgagcatcg aaagcaagtc ataacaaaac gtagtcaatt cgatttagcc aaagctcaaa
     8941 aaagacagca tatcgtagaa ggattgatga aagctttatc gattctggat gaagtaatcg
     9001 caacgattcg tgaaagtaaa gacaaaaaag acgccaaaaa gaatttagtc gacgtctttc
     9061 aatttacaga agaacaagca gaagcaattg tcacattgca attataccgt ttgacgaaca
     9121 cggatatcac agagcttcaa aaggaatccg aaagcttgat tgcgcaaatc acagagttga
     9181 ataaaatcct ttcaaatgac aaagagcttt tctctgttat gaaaaaagaa ttgcgagaag
     9241 tgaaaaaaaa ttattcttcc gctcgtctaa cgacaatcga agacgaaatc gaagaaatca
     9301 aaattgatac gcaagtctta gtggcacaag aagacgtaat tgtcagtgtg acgcgagaag
     9361 gctatgtgaa gcgaacaagc cttcgttctt atagtgcttc caaaccagaa gaaatcggga
     9421 tgcgagaagg cgactacttg ctatactcag gtgaattgag tacattggac catgttcttc
     9481 tgatcacaaa taaagcaaat gtgatctacc gccctgtcca tgaactaccc gatttgaaat
     9541 ggaaagatgc tggtgagcac atttctcaaa caatctccaa tctgtctgta gacgaatcga
     9601 ttttagctgt tttcccatat caaaaaattc aagcagagaa aacatttgta ttcatcagca
     9661 agaatggatt gatcaagcaa acacgaatga cagagttcga accatggcgc acctataaga
     9721 gccgtccgct cagctgtatg aagctaaagg cggaagaaga tgaactgatt gctgtgtatc
     9781 tgaaagaaga tcagccaggc ttagatgttt tcttagtaac gcatcaagga atgggtttgc
     9841 gctatccttt aacagatgtt cccgttgttg gagcaaaggc agctggagtg aagtctatga
     9901 atttgaaaga tggagatagg attatcaacg ggctgcttgt gttagcagaa ggtgatacgc
     9961 cgatcgtaat cgttactcag cgaggagctg ttaaacgcat gcttgctcaa gaaatcagcc
    10021 aaacttcacg tgcgaagcgg ggagtgacgg tcatgcgtga gctgaaaaaa aatccgcacc
    10081 gtattatcta tatgtcggaa ggaagatcaa aagaaataac cttgatgaat caaaaagggc
    10141 agcagttaac gattgaccca actgatttcc ctataggtga tcgaacatcg aatggatcat
    10201 ttgcgatgga tgagaaaaaa ggtggagaag tcatcgaagt tatcgatgct cctgagattt
    10261 tcatcgagtc gtaactgttg tagtatttta atgaagagac gccatctgta ttaatacagg
    10321 tggcgtctct ttttcattca tttttaataa atccttatac gtttgcaaaa aaagatgaaa
    10381 gtgttcacaa tgcttaaatg ttggtatgtc aacgttgatt taaatatatg taaataaaac
    10441 ataaaaattg ttaaataaaa aacaagaaaa cccttgcaaa gatagggtgc tgtgttatac
    10501 taatcacgta aaaactgtta cataaaacag aaatttagga ggtccataca atggagcaat
    10561 ggaatggatt taaaggtacc aaatggaaag aaggcgtaga cactagagac tttattcaaa
    10621 ataactacac tgaatatcgc ggtgatgaca gctttttgga aggcccaaca gaagcaacag
    10681 aaaaattatg ggatgaattg caaaagttaa atgatatcca attcgagcgt aacggtgtct
    10741 atgatatgga cacagaaatc gtatcaacta ttacttcaca cgatgcaggc tatttggata
    10801 aagatttgga aaaaatcgtc ggtctacaga cagacaaacc tttgaaacaa gcattcatgc
    10861 cgttcggcgg aatcaatatg gctaacaatg cgctaaaaag caatggctat acaccaaacg
    10921 atgaattgac acatattttc actgactggc gcaaaacaca taatcaaggt gtatttgacg
    10981 cttatactgc tgaaatgcgt gctgctcgta aaaacaaaat cattacaggg cttccagacg
    11041 cttacggtcg cggacgtatc atcggtgact atcgtcgtgt ggcattgtat ggaatcgact
    11101 atttgatgga acaaaagaaa aaagatcatg atatgactgg ttatcataca atgagtgaag
    11161 aagtgattcg tttacgtgaa gaaatcactg aacaatatcg tgcattagcg caaatgaaag
    11221 aaatggctgc gaaatatggc tatgatattt ctaaaccagc tgcaaatgca aaagaagctg
    11281 tccaatggct atacttcgga tatttagcag caatcaaatc acaaaatggt gctgcgatgt
    11341 caatcggacg tatctcagca ttccttgata tctatatcca acgtgatttg gataatggag
    11401 taatcacaga aaaagaagca caagaattga tcgaccactt agtaatgaaa ttacgtatgg
    11461 tcaaattcgc gcgtacacct gaatataacc aattattctc aggattccct atctgggcta
    11521 ctttatccat tgcaggtatg ggattagatg gtcgttcatt agttacaaag aatgatttcc
    11581 gtctgcttca tacattaaca aatatgggac cttcaccaga accaaacttg actgttttgt
    11641 attctgaaca ccttccagaa ggattcagaa catatgctag cagaatcgct atcgaaagtt
    11701 cttcgattca attcgaaaat gataatcagc ttcgtgaaca atggggttct gatgattgtg
    11761 cgattgcttg ctgtgtatca ggaacagttg tcggaaaaga catgcaattc tttggcgctc
    11821 gtgctaactt ggctaaaaca ctattatatg ccatcaatgg cggagtcgat gaagtgactg
    11881 gcgcccaagt tggaccagaa tatcgtccaa tcacatcaga aatactagac tttgaagaag
    11941 tgaaacataa cttcatgaat atgatggatt ggttagcaga actatatgtc aatacgttga
    12001 acatcattca ttatatgcat gataaatatt cttatgaagc agctcagctg gctttgatgg
    12061 attctgactt gcaacgcaca tttgctacag gtattgctgg gatctctcat gcggcagaca
    12121 gcttgtcagc gatcaaatat gcaaaagtaa aagcaatccg taatgaaaaa ggaatagcag
    12181 ttgattttga agtggatggc gacttcccta aatacggaaa tgatgatgac cgtgcagatg
    12241 aattagtttc atggatcttg gaatacttca tgggacaaat caaacgtcat aaaacttacc
    12301 gcaactcaaa accaacaact tcattattga cgattacttc aaatgttgtt tacggtaaaa
    12361 atacaggaaa tactcctgat ggccgtgaag ctggaaaacc acttgcgcca ggagcaaatc
    12421 caagttacaa tgcggaacaa agtgggttat tagctagcct gaactcaaca gctaaaatga
    12481 attacggctg tgcattggat ggaatttcaa atacccaaac aatcaatcct tcaggtcttg
    12541 gaaaagaaca agatacaaga atcgaaaact tgcgtaatgt cttagatgga tacttctcaa
    12601 aaggagctta tcatttgaat gtcaatgtat tcagtaatga tttattgcgt gatgcaatgg
    12661 aaaatccaat gaaatatccg aacttgacga tccgggtatc tggttatgca gttaaattcc
    12721 gtgacttgac aagagaacaa caattagatg tcttgatgag aacttcacac gacagaatgt
    12781 aaaaagttcg tgaaaaaagt ttttcgactt agcgtcgaaa ggacgttgaa tagccatcgc
    12841 atcaaaatcg aataatcagt atgcttattt gaaagaaacg atttacaggg gcaggacttg
    12901 tatgttccgt ccctgtaaat tttacacata acgaagcggc aattgcgaaa aattttgttc
    12961 agaaagcttc gcttcgacag ctaacttgta cttaaaatga aatcaaagaa aagatgtgaa
    13021 cttaaatgga agaaaaaaca atcggctatg ttcattctat cgaaactttc ggttcagtag
    13081 acggacctgg actgcgcttt gtcgtgttta tgcaaggatg ccgcatgcgt tgccaatttt
    13141 gtcacaatcc agatacttgg aatattggcg gagggaaaga atacacagca gatgaacttt
    13201 tagataaagc agaacgcttc agaccttact ggggagataa aggtggtatt actgttagtg
    13261 gtggagaacc tctattgcag atcgattttt tgatcgaact atttaaaaaa gcaaaagagc
    13321 ggaagatgca tactacattg gatacatgcg gaaaaccttt tacgtatgaa gaacctttct
    13381 tttcacgttt tcaagaattg atgaagtata cagatttact gttatttgac atcaaacaca
    13441 tcgataatga agagcataaa aaactaacgc attgggacaa tgaaaatatt ttagaaatgg
    13501 ctcaatattt gtcaaaaatc aacaaaccgg tttggattcg ccatgtacta gttcctgagc
    13561 gaagtgatta tgatgaatat ttgatccgac tagacaactt tattcaaacc ttgtcaaatg
    13621 tagacagagt agagattctt ccttatcata cgatgggaaa atacaagtgg gaaacacttg
    13681 gtttgaaata tccattagaa gggatcgaac cacctacaaa agaacgtgtc gaaaatgcta
    13741 aacgattgct tcatacagaa gagtatacaa aatatttaac gcgataggtc gcatcggaaa
    13801 gaagataagt attgcttctt tccgtttttt tgctattatt aaataggaga aatcacaaag
    13861 gagcgaatga catgtcaaaa attttagttt ttggtcacca aaatccagat acagatgcaa
    13921 tcggggcagc cattgcgttt tcacacttgc aaaatgaatt aggtaaagaa acagaagcag
    13981 ttgctctagg tacacctaat gaagaaacac aatacgcatt agaccacttt cagctatctg
    14041 caccacgagt agtagagtca gctaaagcag aaaatgcaga tgtcatgctt gtggatcata
    14101 atgaattcca acaaagtatt tcagatatca gcgaagtaaa tattttagct gtagtagacc
    14161 accatcgtat tgcaaacttt gaaacagcta atccattgta ctatcgtgca gaaccagttg
    14221 gatgtacaag tacgattatt ttaaaactat ataaagaaaa ccaagtaaca attcctaaag
    14281 aaatcgcagg aatcatgctc tcagctatca tttcagatac attgttgttc aaatcaccaa
    14341 catgtacgcc tgaagatgaa gcagctgcaa aagaacttgc agcaatcgct gaagtcgatc
    14401 taaacactta tggacttgaa atgttgaaag caggaacaaa tctaagtgat aaatcagctg
    14461 cggttcttct tgatttggat gcaaaaagtt tcccaatggg cagttccaac gttcgtatcg
    14521 ctcaagtcaa tacagtggac cttaatgaag tgatggatcg acaagcagag ctagaagcag
    14581 caatggaaga tgaaatgaaa gcaaatggct acgatctgtt cgtattagtt gtcacaaaca
    14641 tcttagacag tgattcagaa ttacttgttg ctggtaaagg aaaagaaaaa gtagaagaag
    14701 ctttccaaac aactttgacc aacaaccgtg ctttgcttaa aggtgtcgtt tcacgtaaaa
    14761 aacaagtcgt acctcaattg acggctgtat ttagctaatc aaatggtagt atgaagcatt
    14821 caaaatgaat ggataagcga ttgatataaa aagaaacata gaacatacta aaggccgaag
    14881 aaatggagga gaataacagt caattatgac tcttgttctt cctccttttt acgtaaaagc
    14941 cttttaagta aaataaaacg gatagaaaag agaacatata tgaactatta ttcaaaaaat
    15001 aaggaaaact tttatcaaaa actgaccgat gatccgcttt tttcttcatt gaccgattat
    15061 ctatatgaac atcgagaaca agaaacgatt ctaagagaat tgaaaaaaga attttcgcaa
    15121 aataaatttt cccattttct tgatttattg atcgatgcag gattaatcaa gagggaagaa
    15181 cgaagatatc atttgaactt tccaatattt gatcctaaag attacttgca acaagccact
    15241 tcggctgctg aaaccatagc tgaacaattg aagagactct ctgttgatga acaaaaatta
    15301 gcgatgggag aagttatttg ggcgtattgt ttcgaagatg aacggaaaga agcgtatttt
    15361 tatggagtaa gaaacagccg agagacagaa ttgctgagga caactgcagg aaatgagaag
    15421 taccgtttta tcacactttc ctctatagaa cacttcccgc ttactttggc taattacttt
    15481 tttgtacaga agaatcagct tcctgtaacg aaggcattca aagaattagc cgaacttatt
    15541 ggagacgtaa atgaatcgta cttttttgat caaatagaag ttatcgttga caggatacgt
    15601 aagaataaat ataaaaatcg ccggcctagt attttccatc agtctctact tgtgacgaat
    15661 actataaagg aagaagaatc attcacatta gaattaccta ttgtcgaaaa aaacaatttt
    15721 gagattgaat taccaacatt ggatccttca ttgacaatgg aggaaatggc ctttcttaaa
    15781 cgacaaatct tttctgagct gagcaagaaa tttataccgc atgcattttc ttatatcaaa
    15841 gaatatggaa cagttttagt gagtaaaacc taagagagca gtaagcaaaa attatactga
    15901 ctcttgcagg aggagccttt ctcttccttt atggttaaaa gaattatttg agtattttgc
    15961 ctatctacca cctattagat tcactacatg gtctggaaac aaaggaatgc aaaaaaatgg
    16021 tgagaaatgt ggaatgacaa aggaagtagt gataagaaaa gttagatttt taaataatca
    16081 atattatgat tcagtcaagt atggcatttt atgggaagaa ctagctgatt agattaggaa
    16141 gtccatttga taatccagaa tcactaagaa acgaatggcg agtcattaat aatttatcca
    16201 atcttgggtt attcttttag attgaagtgt ttaaaactaa aaaagcattt atgaagtcgt
    16261 aatagtcagg cttcaaaatg ctttttattt tgtttgtttt ttctttgcca cataaataca
    16321 cctagactga cgatgaagag catatccaac agatttcctg cgacttggat cgtcgaaggg
    16381 ttggtcagtg gcattaaagc aaggacaaaa aaggaaaaca agctgtaaat aaaacaggtg
    16441 acaggaacta atcgaaagaa gagcggacat ttcaattggc taaaaatgag tgaaaaaata
    16501 aaataagtaa aaatggataa aatcacgact aaaccattca tgataaagaa gaaaagcgta
    16561 ttgtgattaa acgaataaaa aagaattgct aaaaaagaaa acgttttaaa tgcaatcaat
    16621 acgcttgaag cttcgtttaa cgtaccagga tgtcgatgaa aaaaataggc accgaaataa
    16681 acagaaaaca gtaagccgca aagagaagac aaaatcaaaa aaaacggatt ttcttcggct
    16741 tctgtgatat cagaatgatc caaaagcaac cagcctaatg cactagaagg cgcatgtgag
    16801 gtcttaaggt atgaataaca tagaaaaaat aagatcgtca aagttgcaaa aaaagagcct
    16861 aaatgtaaaa aattagtggt tctacgttga atttccataa tatctccaaa ttaaattgat
    16921 aagtatataa agtataataa caaattgatg agtatatgtc gatgtttttt aatcaaaata
    16981 ataaataaaa actgtctaac atttaaaatt taatttagat attagacagt ttttaagttt
    17041 gtgagttatt ttccttgtcg ttttctcact tttttagtcc agaaaccacc tgaaatatat
    17101 tttggctcat aagaaatgat aaaagcacgc tcttcgattt ctttgatcag gcgatacagt
    17161 tcacgttcgc tttttctagg agaaagtatt tcgagaacta gccgctcacc ttccagcccc
    17221 attgcagaag tctgtgtcac accataaccg tgttctctca gtatacccgg caaactttta
    17281 tcttctgcgc tatttgctgg caaaatcgtt gtgaccatga tgtatcctaa tgccaatttg
    17341 tcttcaatca aaattccgac actgatacct actgcgtaac ctaacgcata gacaaataaa
    17401 ttcagtggat tatctaaacg atctaaaaca agggataagc cgacgatata aatcgtgatc
    17461 tcgaccatac tgacaagtgg agcaatcagt cgatagcctt tcattgtcaa catgaaacgt
    17521 aacgtgttca gagtgatata agcaaaatta atgaggaaga tcatccccaa catttttgga
    17581 tcaatcattt gtaaaactcc tttcatttat ataacaacaa tatcaaaaaa aatcgtaagt
    17641 gaagaaaaat tgaaacttta tacatacaaa aaaatgataa aatgattata atagaattat
    17701 aaagataggt taggtgatta aaatgaaaac ggtcaatcgt gtgttttgtg ccagccacgt
    17761 gctaagtctc cttctcagta tatacttagg tgtctattcg attcaacagc caattgaggt
    17821 agagaacatt gtttttcaga tgtcgctgac agatggttta attttaagcg tttttttctt
    17881 tttgatactg tttattggta atattttcgg tagcgttgcc agttttttga atttttctat
    17941 ttatccactc ctttcgctag cattaggagt ggtgggactt tgttctctgt gcttatttcc
    18001 cgagccattc agtcctgcat ttatattatt tggtacactg aacctgttcc agacaactgt
    18061 tggagcatgg ttgctatggc gatcaggaaa tttaatgaaa ataggtgagt aacaattgaa
    18121 tccaattata ttgaaacgtt ctgtccatca tattccagtg ttagaagttg ttctgaaaga
    18181 attaagagag gaacgtattc ccgttgtcat ctattatcac ggatggcaga caagtaagga
    18241 gttagttttg acgcaaggaa gaaaaattgc gcagcagggc attcgtgtga tcctgccaga
    18301 tgcgatgaat catggggagc gcaaacagcc ggtttcttct attccatcct atacattttg
    18361 ggatagtatc tacggaaatc tgtttgaatt tgacacactt atcaagcatt tagaaaaaag
    18421 aggtcttcta catgatcagc ttgcagttgg cggggtttcg atggggggaa tgacaacatg
    18481 cgccttgttg gctaaacatc ctcatatagt agcgggcgtg tgtttgatgg gttcccctgc
    18541 acctttggaa tatggaaaag aaatcgtaag gcgcgcatcg gaatataatt atgtcgtacc
    18601 aaaagactat tttgatttga tttcatgggt taagagctat gatttgtcat tgcagccgga
    18661 aaagttagct ggacgtcctc tgttcttttg gcatggacaa gctgacgaaa aaatccctta
    18721 tatacaaacg gaacgatttg tagaaaaaaa tcggcataag tcttacggaa aagaaattgt
    18781 ttttcgttcc tcaaaaacac agcgacatat ggtacaaata ccactaatgg aagaagcttc
    18841 cactttttta gcaaaaaatt tgataaaaaa gtataaataa ttgaaacgga aaatcactca
    18901 tgacgataga ggattttccg ttttttattt tgggctataa taaactctgt taggaaaatt
    18961 tggtataatt gatagtaaga atgacaacat gttagaaagg aagagagcat gcccacttta
    19021 gcagatgttg caaaacgtgc caatgtttca aaaatgacgg tctctcgagt catcaatcat
    19081 ccggaacagg tgactgacga attgaaagag ttagttttct cagcaatggc tgaactggat
    19141 taccgtccaa atattgctgc aaaagcactt gtcacgaatc ggtcgcagat catcaaatta
    19201 tttatactgg aagaaatcga cacaactgag ccctattaca tgaatttgct gatgggaatc
    19261 gctaaacgga tcggtaaagc gcattattct ctccaattag ttacaaatga tgccttcgac
    19321 gtcggttctt gtgatggata catcatcaca ggtgtcagaa aaaaagactt cgattggatc
    19381 gaccgtttgg aaaaaccagt tgtcttgttt ggggaaaatt ctgaaggata tgattatgta
    19441 gacagcgata atgaatatgg tacagcaaaa gcaacgcaat atgccttaga aagagattat
    19501 caaacgatta tttatatcgg tatcgaagtg gacgagcctt ttgaaatttc aagggaaact
    19561 ggttatttat ctgtgatgaa ggaagttgat cttcctagta aaatcttgcg gtttccaaat
    19621 cattcgacag aagtagaaag atatatgaca gaaaactggg cagattttac agataaaact
    19681 tgttttgtat gtagttcaga tcggctagca ctaggagcag taagagggat tataaatgct
    19741 ggaggacact tgcccgaaga atttggtgtg attggatttg atggggtatt tttagaccaa
    19801 atcagttcac ctaaattgac aacgatcaag caagatatta tacgtatggg tgaagtatgt
    19861 ggagaaatgt tgttaaaaaa aatagaagaa aaaggggcga gtcaaggtta tcgtcgtttt
    19921 tttcctgaac tagttatcag agagacaacg aattaagtct tattactata tatatattta
    19981 tgaaattcat cgctattttc ttaaaataat cagcgcattg ctactgaaaa caatgaatct
    20041 gtgctattat tgagaaaagt tccatttttg agaaggatgg tttgaatgaa agaaatcaga
    20101 cgaaaacatt tccgttttcc tgcgtacgat gatgagttag gcgtaaaatt aaccaacgac
    20161 aagaagcgag tattatttga cggacaagaa gatcttttga ctgaacagaa tcagtccccg
    20221 tttgaacaga tgaaagaatt tcatttcacg aacacgaaca ccgagaaaat ccgtcgcaag
    20281 cgtcgtgcaa caaatggatt gaagtcagct ccgcgtaagg aaaatttgga aaagcataaa
    20341 ggaaaattac cggattatac cggccattac caatcagaaa tcacatcgac aggacgaaaa
    20401 aaactttttg gtgattcctc tcgttctgtc agacaaagtg aaacaaggac agagtccaag
    20461 catactacac gaaacgcatc tgttgacacg aaggtgcggg agcgttctta ttttgttcct
    20521 aaatatgtac ccgcatcgat tatcccagac cctagagaaa gcaaaatttc tgaggatcat
    20581 ttaatcgaat cgatgaaaaa aacggatgac tcttatctgt tgttcgatac agaaccgacg
    20641 ccgtatcaag aaaaaagaaa tggtgagccg tctgtacgaa cattcaatcg aacaaaaacg
    20701 ttagaggaaa caaagcagtc agaagaaaaa aaatcgaaaa aaagccatgg tatcttagaa
    20761 cgctcattac aaggattgat cgatgatcaa ggaaactctt tgggagaaaa tagctatttt
    20821 aaataagtga tatacaaatt tatattcggc tacagttgga aaggaaacgc tgcggcgttt
    20881 ccttatttta tactctatcc gaaagtttat atcgtatcct caaatttttt tgagtttaag
    20941 cgaatttttt ctcccaataa gtaagaaaat aatggcaaaa taaaaaaaca aactattttc
    21001 gcagatcgtt tgcaaaaata gtt
//
LOCUS       DACDMM010000034        21018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDMM010000034 DACDMM010000000
VERSION     DACDMM010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.034"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            241..1413
                     /locus_tag="IWD16_000967"
     CDS             241..1413
                     /locus_tag="IWD16_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9088049.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            1400..2104
                     /locus_tag="IWD16_000968"
     CDS             1400..2104
                     /locus_tag="IWD16_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9088050.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            2109..3269
                     /locus_tag="IWD16_000969"
     CDS             2109..3269
                     /locus_tag="IWD16_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9088051.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            3345..3623
                     /locus_tag="IWD16_000970"
     CDS             3345..3623
                     /locus_tag="IWD16_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9088052.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            3604..3975
                     /locus_tag="IWD16_000971"
     CDS             3604..3975
                     /locus_tag="IWD16_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP9088053.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            3991..4395
                     /locus_tag="IWD16_000972"
     CDS             3991..4395
                     /locus_tag="IWD16_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088054.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            4392..4802
                     /locus_tag="IWD16_000973"
     CDS             4392..4802
                     /locus_tag="IWD16_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088055.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            4863..5450
                     /locus_tag="IWD16_000974"
     CDS             4863..5450
                     /locus_tag="IWD16_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9088056.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            5523..5990
                     /locus_tag="IWD16_000975"
     CDS             5523..5990
                     /locus_tag="IWD16_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAP9088057.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            6036..6344
                     /locus_tag="IWD16_000976"
     CDS             6036..6344
                     /locus_tag="IWD16_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088058.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            6539..13273
                     /locus_tag="IWD16_000977"
     CDS             6539..13273
                     /locus_tag="IWD16_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9088059.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            13277..14149
                     /locus_tag="IWD16_000978"
     CDS             13277..14149
                     /locus_tag="IWD16_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088060.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            14146..14773
                     /locus_tag="IWD16_000979"
                     /pseudo
     CDS             14146..14773
                     /locus_tag="IWD16_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14734..15195
                     /locus_tag="IWD16_000980"
     CDS             14734..15195
                     /locus_tag="IWD16_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088061.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            15199..16023
                     /locus_tag="IWD16_000981"
     CDS             15199..16023
                     /locus_tag="IWD16_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088062.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            16041..18083
                     /locus_tag="IWD16_000982"
     CDS             16041..18083
                     /locus_tag="IWD16_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAP9088063.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSIDFGGYD
                     YSGKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTY
                     TLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVN
                     YQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLG
                     ENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNF
                     GELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQIDWLKIEKGDTRTP
                     NISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSIEGLKNFEDGIQS
                     KGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGNDSTILT
                     IPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            18243..18689
                     /locus_tag="IWD16_000983"
     CDS             18243..18689
                     /locus_tag="IWD16_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088064.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            18691..18828
                     /locus_tag="IWD16_000984"
     CDS             18691..18828
                     /locus_tag="IWD16_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9088065.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            18865..19158
                     /locus_tag="IWD16_000985"
     CDS             18865..19158
                     /locus_tag="IWD16_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088066.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            19155..19379
                     /locus_tag="IWD16_000986"
     CDS             19155..19379
                     /locus_tag="IWD16_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9088067.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            19376..20395
                     /locus_tag="IWD16_000987"
     CDS             19376..20395
                     /locus_tag="IWD16_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9088068.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            20584..20657
                     /locus_tag="IWD16_000988"
     tRNA            20584..20657
                     /locus_tag="IWD16_000988"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20618..20620,aa:Ile,seq:cat)
BASE COUNT         7471 a         3311 c         4443 g         5793 t
ORIGIN      
        1 ttgattttga aactaatcaa cattttgaaa caagtatcaa tgaacagtgg tttctatcac
       61 tatcaactgt tgacagtgat aactctataa agcgacaaaa attatctgaa tatattaaag
      121 aattaggatc cgtagaatat aaaaaaatag ctaattttga aagtttatat aaactcttta
      181 atgaaactaa gctctagaaa tagggctttt ttatttttct ttgaaaggtg gtgaaaacat
      241 gtgagcttat ttgatatctt taagcagtca ataagaaatg aagagccttc tgagtggata
      301 ccggattttg tatatggaga ggaagtatct gctcgggcat acctcaaaat tatggcaaaa
      361 aatactgttt tagattttgt tgcaagaact atgtcaacgt tagaagttaa gtttaaaaat
      421 aaagatggga catcagactg ggattatatt ctaaatgttc gacctaattc tgatatgtca
      481 gcagctacat tttggcaaaa atttttttat cggctcttag atgacaacga agtgttagta
      541 atatttacag atgataatca attgctgatt gctgatgatt ttagtcgaac ggagtatgcc
      601 gtgtatgatg atgtttttac aaatgtgatg gtaaaaaatt ttgtctttga acgttcatgg
      661 aacatgtctg acgtcattta tatggaatat aataacgaag aattagaaag gtttacacga
      721 ggtttatttg aagattacgc cgcgttgttc ggacggattc tagaagttgc aatgcgtaac
      781 aaccaaattc gtgggtctgt atccattgat tcaacgggaa cggcgaatga tgaaaaagga
      841 aaagatggta agactagatc tcaaagatta caagaatata ttgataggat ttggaactct
      901 tttaaaacta actcagttgc aattgttccc aaagtgaaag gctttgagta tgaggaatac
      961 accaataaac aaggcgtatc gaatcaatcg ctagatgaac tgaataagat gaagtcgtct
     1021 ttaattgatg atgttgccaa cgccatagga gtacctacgg cgcttattta tggtgaaaaa
     1081 gctgaactgg attcaaatat taaagctttt cgtaaattgt gtattgctcc actagttaaa
     1141 aaacttcaag acgaattggt ggcaaaaata attagtcgtc aagagtacaa aaacggggaa
     1201 cgtatagaag ttaaaaaagt tctccaaaaa gatctattag agtatgctac tcaaattgat
     1261 aaaataacat ctggtggtgc gttttatatt gacgaagttc gtgatgaagt tgattatgat
     1321 gagttgccaa ataatgaagg aaaaattcgt attcgtacga aaaactatga agaaactgtg
     1381 aagggaggtg agaacgacaa tgccgaaagc taaaaaagta ccatttcagt ttactaacga
     1441 aattcagaat ggtaagcaca ttctcacctt gagtggaaat gtacagaaaa aatattggcg
     1501 tgatgatgat gtcataaacg ctaaagatat tcgtgactct ctagatactg taaccgatga
     1561 tattgtaatc aagttgaaca gcccaggcgg tgatgtgttt gaagggattg agatttacaa
     1621 ttatttaaaa gatcacccat caaatattac tgtagaagtt acagggcttg ccgcaagcgc
     1681 agcaacgttt attgtcgctg gtgccaatga tgtgattatg aatgttggga cttctttgat
     1741 gattcatgaa gcttctacat ttgcttgggg gaataaacag gacatccaaa aaacgttaaa
     1801 cgcattggaa acgatcgatg attctatctt agctatttat tcagataaga ctggccaatc
     1861 tgttgataag ttgcgtgaat ggatgaatga agaaaaatgg tttacagcag atgaagctgt
     1921 caaatatgga tttgccaatt ctgtgaagcg tgaagaacca caaaatgaac ctcaaaatat
     1981 tgcagcgatg attcaagatg ctgttgccgt tgcaatagcg aatttaaagg aacctgttac
     2041 taataaaatc gaacaagaat caaaacaaaa atcattaatc gcacgattgc gaaaaggaga
     2101 ataaagatat gttaaaaatt acagataaaa ctgcagatgc aaaaaaggca tttaatgcta
     2161 tttcttcgaa agaagatgca acacccgaac aaattaatgc tgctctagaa gaatatgtaa
     2221 ccgccattgc agaagatgcc ggcaaacagg tgcgtgctga atatgaagaa ttgaaaaatg
     2281 tgacggacaa ccgtgtatta gaggctcgtg gaattcctac actaacagca gaagaaacaa
     2341 agttttataa tgaagccgta aaatctggag gtttcgattc tgacgtagtt tggccagaaa
     2401 cgattttgga acgtgttttt gaaaatcttc aaaacgatca tcctatttta ggcctcatta
     2461 actttacacc aactgtaggt cgtgtcaaag taattagagc acgtcgtaaa ggtgttgctg
     2521 tttttggacc attgcataaa gaccttgaag gccaactaga tgctgaattt ggtgcaactg
     2581 aatttgttca acttgcgttg accgcatttt tcttaatttc taatgatacc ttagatttag
     2641 gaccacgatg gattgaccgt tttatcaatc tttctttaag tgaagcagta cgtgatgttt
     2701 gggctaaaaa aattattaca ggttctggta aagatgagcc aattggattg cttaaagatc
     2761 tagatggtgc ggtcacaagt ggtgtatatc cagataaagc tagcgcaggg acattaactt
     2821 ttgcgaaaga gaagattgtt cttgaacttg caggggtgat gaaaaaatta tctaaataca
     2881 catacaaagt agatggtgac gatgaaggtg aagtgaaata ccgttctgtt gctggacgag
     2941 tgtatctaat tgttaatcct gttgattact gggatatcgt agctagagtt acatttgcaa
     3001 atttaaacaa tgtgtatggg actacgatgc catttatcgc tgttgatcat atcatcgaat
     3061 ctgtagacgt accagaaagt aaatgtattg catacgttga taaagagtat gaagctactc
     3121 aatcaagagc tgaaaaagtc tatgtatata aagaaacttt tgcaatgaaa cgcgcaactt
     3181 tgtatgctgt cgacatgctt ggtaatggct atccaacaaa taatgatgca gcacaagtat
     3241 atgacttgga ctttacagcg ggggagtaat gtcccctgca attggtgaga taacaccgac
     3301 gactgatggg gcagtcatta atctttcgta aatggggtta gagtatgaac actgaagaat
     3361 ttataaaaga atataaggcc cgatttcgta ttttccatac ttccgaagat gaggatatcg
     3421 ggaaacagtt agagagcggg tttgccgata taaaatctct aatcggcagt tttgatcctt
     3481 ccacttatga gaaaggaaaa gaattagttt atgaacgtac tcggtatcta cggaacgaat
     3541 cactagagta cttttacgac aattttcaaa caatgattat ggatgcatca attgatctgg
     3601 caggtgctgg atatgccaat taaatcaaat tataagaagc ctaaaattgt agctggtgat
     3661 ctaaatacgc cagttacttt ttttgagttt caacctaata agggtccaga acctggtgat
     3721 gaagaaaaaa aggaactgta tttttgtaca gcactagttt acaacccatc aatgaaagat
     3781 cgtgacattc ttggcacaaa agaaggcgtt acgataaaaa tacgggatcc ccatcaatct
     3841 tatattccta ctaacaaaca taaagtaagg atattagatt atcgatatgg agataaagtt
     3901 tgggaaataa tcgatgtctc accggatttg gaagaaaatt caattgtaaa gatagtatta
     3961 gaattaatta cttaaatttg gtgataaatg atgagtaacg taaagtttga aggcgttcaa
     4021 gaaacaatcc aagctttaga aaaaaagtat ggagaaaaga aaactaagac attaacaaaa
     4081 aaagcaatca atgttggcgc tgaaaaagta gaaaaacagt tgcaaacgga catgaccgtt
     4141 tttaaagaca aaggttatac gatcgctgag gttgttcgca agaacgctac gtatagagat
     4201 tacaaggcag aagctgaaat cggttggaat ggtccacatc aacgatatag aatcattcat
     4261 ttgaatgaat ggggatatac acgaaagggc aaacaaatta gaccgagagg atttggtgta
     4321 ataacaaaat ctcttaagaa ttccgagctg ttgtactttg atgctgtagg agaggaggtt
     4381 aaaaagagtt tatgaaagat atgcttaata tcatttacga ggctctttgt ttgaatgagt
     4441 atattcattc aatgacctac aacgagaata ccgagcaata tcgtattaaa ttttatgagc
     4501 aacctgaaac cgccgataaa acaggtccaa tgatcacaat tcgtcctgta gatacaccta
     4561 acgaagcgta ccacggaagt aacaaagagc tttctgtgga gcatatgata cagattgacg
     4621 ttgaatcgaa atatagagca acatgcaagc agattcaata tgagataaaa aaagagatga
     4681 aaaaactagg ctttggccaa atttccggtc aagggttaga tgaatacttt actgaaacaa
     4741 accgctttgt agacgcgcgt cgttacgaag gaaatacaaa aatttacgat accgaatatt
     4801 agaaattaag acacgaaaat aacgtgtctt tttttattac ccaaaattag gaggaaaatt
     4861 ttatgacttt agtaggattc aaaaaaatga caatcggggt ctttgacagc acagggaaga
     4921 ttccagcagc aaatttatat gtaattgaag gtgaacaaaa caaaggggcg actgtatctg
     4981 ctgaaatcag tgggctatca aaagaaccat caaaagttta cggatcgaac atcgcttact
     5041 atgtctctca aaaaggaaca ggcgatgttt cagctacttt tggattgtta gatttaccaa
     5101 cagaagtaaa tgacaaaatt cttggttaca aaaccgatac aaacaaaatc agcttcttgg
     5161 gagaagatac tgagccacca tattgtgcaa ttttgatgga atcggaggat ttgaacggcg
     5221 atacagcaat gcttgcgatg ttcaaaggaa aattcagtcg tgaatctatt aatcttaaca
     5281 ctacgacaaa tgaagctttc gaaccagaag cagaagaata tgtattttca gcgattgcca
     5341 atgatgttga aggtgatgct aaaggccaaa cagtcgcaaa atatgttgga gacgaagaag
     5401 cttctattac tggtttgaaa gagatggtat ttcctgcagc gggggagtaa ctagccccgt
     5461 tgttggagca atcactccca ctattaatgg ggcaacagtt gaattaagtt aggaggataa
     5521 ctatgccaga tacatttaaa atctataaaa aaaatggtac tgaatttaca aaagtcgcag
     5581 agggtgaaag tccactttct attactggaa ttgcagcaaa tactcaagtt gctaaaggag
     5641 attatcaagc tacgcgtgtg gtcggaggtc aagaatcagt aaaagtagat attcctgcat
     5701 tcaccactct tccaatttca gttactggtg tgacgttgga taaaacgaca gctgaagttg
     5761 aacaaggcgg aaccttaaaa ttaactccta ctgtaacacc agcaaatgca acagacaaaa
     5821 caggaagctg gagcagttcg aatacagctg tagctacggt tactggaggc aacgttacag
     5881 taaaaacaga cgctgaagtt ggcggaacta cagaagtttc atttactact acggatggcg
     5941 gtaaagtagc aaaatgtact attacagtta ttgaaaaagc agcgggctag tcttcggact
     6001 agctttttta tttggaaaat aatggaggaa tctaaatggc aaaagtaaga attgaattga
     6061 aagaagaatc aggaaagaaa gtctatgaaa acatagacac aacagggaaa gattatcgaa
     6121 aagctttaga aacaatcaaa aagctaaatg aagacggagt aatgatttgg aatcgtttag
     6181 acatttattt ggattttgct gtaggtatct tcagggatag caaattgaca gctgaacaaa
     6241 ttcttgatgg cttaccatct gagaaggtaa tgagcacatt ggacgacatc ctaggagaag
     6301 taatggggat tgaaagtaat ccagacccag acgcaaaaaa gtaacacccg aagaagcaga
     6361 agaaatgtac ttaaacctat gcagagagtt agtgaaagcg ggatggtcac tatctgacat
     6421 agaagataat tcctttgaaa tgatgatgaa aattgcgtgc acttcgccta agaaagagaa
     6481 acaaaaagaa gttgatctta aagacttctt gaaatccatg tagagaggag gtaaacatat
     6541 ggcaaatgga aaacctttag gcaatatgat tatcaaacta gacttagaca gttcagcttt
     6601 ttcaaaaggt ctagccggag ctaaaaacgc tgttaaccat caaatgaaag caatgaaatc
     6661 acagatgcaa gtaatgaact cttcaggaaa ttctctcggt gctttacaag caaaatacaa
     6721 tggtttaggc ggagttctac aagcaaatga aaagcaagta gaattgctca ctaaggctta
     6781 taaggatagt tttgactcta acggaaacgc tactgcttcg actgctaaat acgctaatca
     6841 attgaatcaa gcaacagcta gatcagcaag ttacgaatct cagatgaaaa caactgttgg
     6901 acagattgct cggatgaaag tggaaacgga agggtttacg gggaaactaa aggctaattc
     6961 tgatgtattg attaattctg ggaaaaaaat tgaagccttt ggtaaaggag tttctgggat
     7021 aggatctgcc ctaactgttg gagtaacagc tccaatagtg gcaggcgcaa ctgctgttac
     7081 tagtgcagct atttcatggg aaagtgcttt tgcaggtgta aaaaagacaa atgatgaagt
     7141 tgtagactcg aatggaaacg tagtttattc ctatagcgac ttagagaaag gtctgcgtaa
     7201 tcttgccacc caattacctg gtagccatca agaaatagca aatgtagccg aagcagccgg
     7261 gcaattaggt ataaagactg aaaacgtagt tagcttcact aaaacgatga tagacatggg
     7321 tgaatcgact aatatgtcgg ctgaaactgc ggcaacttct ttagcacgat ttgctaatat
     7381 tacccaaatg tctcaaaaag actttgataa attaggatca gtcattgttg atctaggtaa
     7441 caattttgct acaaccgaat cagaaatcac tgaaatggga ttgcggttag cgggagctgg
     7501 taaacagatt ggaatgagcc aaggcgaaat tctaggattt gctactgctc tatcttctgt
     7561 tggtgtcgaa gctgaagcgg gtgggtctgc cttttcgaag gtcatgatcc agatgcaact
     7621 agctgtagaa aaaggatttg gcgcttttga tcagctgaaa cagatggcag aagaacaagg
     7681 tgttccgtgg gttaatttag taaatgctgt gagagacggt ggaaaatcgt taaaagcagt
     7741 atcagagcaa atgggattca cttcatctga tttaaagaaa atgtataaag aagcggacaa
     7801 ttcgaaaagt tcgcttgaaa attttgcgaa tgtggcaggt gttacaagtg aacaattcca
     7861 gaagttgttc aagagtgatc cttcagaagc aatcatcaag ttcattcaag ggctgaaaga
     7921 ttcagaaaaa catggtactt cagcaatcaa agttctggat gacatggata taaaagaagt
     7981 tcgactacgc gacagtttat tgagagctgc aaatgctagt gacatattca aaggagcaat
     8041 tgaaaaagga aacggcgcgt ggaaacaaaa tacagcttta acaaatgaag ctaataaaag
     8101 atacgaaaca actgaatcaa agctaaagat attgaaaaac gaagtgattg atacagcaat
     8161 cgatcttgga ggtccatttg tagacgcgtt gcgtgatggt ctaaatgtat cgaaaccatt
     8221 gataaaaaac ttagggaata tggcgaaagc tttctcagct atgagtgatg atcaacaaag
     8281 aaacattatc aaatgggttg gattagcagc agcagctggt ccagcgctga agcttttagg
     8341 aactggtact tcagtaatag gtaaagttac ttcaactact ggaagattga cgaaaagcct
     8401 tgttgattta gcagcgaaag cagcagaaaa gaaggctatg gatgcctttg catcgtcggt
     8461 aacaacagct ggtgctgcag ctgcaaagac agcaggagct ggaggtattg gcgggttagt
     8521 ccctatgatg ggaaaaacag ctagtgctgc aagtactgct gcgggcgcca gcggaatagg
     8581 cgcaatgaca acgtcactag gtttgttagg tcctgctcta cttggtattg ttggcgttgg
     8641 tggcgcactg gcagttggct atggagcatg gaaattattt ggtgaagaag cttggaattc
     8701 ctcgcaaaga gtaaagcaat ggggaacaga tgtcggtgag gagatagata gtactcttga
     8761 cggagtacag aagaatattg aaggtgcaaa tggtcaattc ggtcttctca aagagggttt
     8821 tactgttgat gatgcaaata atatggcggc taatttcgca accgctggaa catctttgga
     8881 aacatcttta acgggtagaa taaaggcatt agacgatgca ctaaaagggc tacctcaatc
     8941 agttcaggat gccatgaaag aagtagctga atcagaagaa gaaaccttag ggcaatctct
     9001 ttctaaaata aaagacaaca atgaagaaat aaaaaaaatc agagaaagag ccgcaaatca
     9061 gaacagagac ttgactgttt cagaactaca acagatacag gatctaatga aaggaacttc
     9121 tcaagcgtat gtggatacgc tcgatgtttc tgctaaagac agaaaaaaga tactatcagc
     9181 catgaatggt gatgttgcac aagctacaga agaagaagcg aagacgtggt tacaatcttt
     9241 agcgaaacaa aaagctgcaa ctaccgaaaa ttatagacaa atgcttgaag aaaaaaaggc
     9301 ggctttaaag gaaaagggag taagcaaaga attcattgat gcattagaag atgaaatgaa
     9361 tgattacatc aaaactacaa atcaaggatt cgatagccaa atagctgcta taacaaataa
     9421 atatccacaa ttgattaacg aagtgagttt atcaaatggt caattattta gcaaaacggc
     9481 agctgctatg gacaatactg gtcagtatac aaaagttttt ctcgaaagta atgaaaagat
     9541 tttagcccaa gcgaagaaaa catcggatga aatagcagat aatgccgaaa aaaatgcaga
     9601 acaacttggt atggtagctg atgaggctac gcatgctgga tcaacttgga atggcttagt
     9661 tttagacgaa aaaacaggaa aagtaaaaac taatgtacaa gaagtagtga atgaagcaac
     9721 gaaggatttc actaaatgga atgaattgaa acctgttgtt catgatgcga aactaacaac
     9781 gaatgcaaaa gatgtcattt ccatagcggc agtacaaaat ggctattggg atgatatgga
     9841 atggaaagaa aaagaagcat tattagaaga tgagtgttct aaaaatgtat acaaggcttt
     9901 agaaaatgct ggtaaatgga atgagttaga tattccttct aaagaagcaa tcctaacttc
     9961 aaatactcct gaagttatgg gtgaaactct catgaatttg ggattatggg atacttacaa
    10021 acttcaagtg aaagatttag atttgaatgc gtataaagta tacgatgcta tcgatggatc
    10081 agaagaaaaa ttaaatagtt gggctcaatt agataacccc acaaaagaat tgttattaga
    10141 taactcagat ttttctacaa aactttttaa ttccgaagaa atgtttaaac gttggaatca
    10201 gttatcatct aatgcaaagt ttctttcggc taataatact aaatttacag aggttctttt
    10261 atcatctgac agaatgtgga atcaatgggt aaaccttcct gataatgaga aaagattact
    10321 tgcagataac gaagatttag ctaccactgt tttatcttca gaagaaatat acggaagatg
    10381 gactcaatta ccagataatc aaaaatatat gttagctgac aatactgact tagcaaacaa
    10441 aatattcgct tcagaagaat catggaatgc atggaaagca attccagaca cagttaaaaa
    10501 cttgcttggt aataatattg atttactggc taaagtgaaa gacgggactg taagtgtaga
    10561 agaatataac aaagttatgc cacttcttaa aactctatat ggagataacg ttaatttaaa
    10621 agaggcaatc agtggggcta aaagctctat agagaattac aacaaaaata tttttcctag
    10681 tgataaaact gcagtaggac acgatgaggc ttcacaagct gctaaagatg catttgaagc
    10741 gttcaacatc ttccaaacaa aaataccaga taaaattact aaaactgtat cagctgactt
    10801 tataggtcct atgccgaaaa atgcgaaagg aacaaacttc catcctggcg gagcagctat
    10861 ggtaaacgat caaaaagggc ctacttatga agagcttata accttgccaa gcggagaaac
    10921 ttttatacct aaaggacgta atgtcgtttt agacttacca agaggttcga aagtactgaa
    10981 cgccactaaa actaaacgtc tagtgcctaa atatgctgat ggtataggaa acataacgac
    11041 ggtttcatct acgctaaact tagatgcttt aattttagct attaacgagc taacgacggt
    11101 attgagaact caactgatgg caggaacgac tgatgctaaa gatactaaac tatcagatga
    11161 aacaaaagtt gccgatccga ttattccgga cagtctttct gaaaaatcag atcagtatct
    11221 aggaattgga gctcaatggt taactaatct aatgaatggt tggaactcag cggttcctca
    11281 atatatgaat agtgaaatgg tttttattac aaactatctc aatgcgctaa aacttcaaaa
    11341 caatcctaat tatacgcaag gagcaacatg gaataagaac ttactgaatg gatggaatag
    11401 tttaactggg acctttattg ctacaatcaa ttcattttgt aatcaagcaa tggtcacgct
    11461 tagaaactac aacactccta tgtacaacaa tggccgaacg tggcagcaaa ataatcttaa
    11521 tggttggaat tcattgtatg gatcattcat agcccgtgta aatcagcttg gtaacgattc
    11581 gattaacaac cttcgttcta aaagtggtgg tttttacaat gcagggacat tcttgttgca
    11641 atctttaata aacggaatga attcgttagg taattctctt tctactacaa tgaacaatgt
    11701 cgctaataca atggttggtg gtataggcaa aggagttaat ggcgtcatat cgggagttaa
    11761 ctatgtgctt aaggaagtcg aatcaagtaa gaatatcgga aactggccaa ttccacaata
    11821 cgctaaggga actgatggac atcctggtgg acttgctatg attaatgatc aaaaaggcct
    11881 agttcacgaa gaatatgttc agatgccaga tggacgtagt tttattgcca aaggtagaga
    11941 tcttcttgtt aatttgccta aaggtgctca ggtcttaaat gcttctttaa caaagaaatt
    12001 aaaggaacgt ttaaatattc cacactacga aaaaggtatt ggcaatttag atattgttga
    12061 tttgttagat gatgaaaaaa gaatgttaga attcttaact agcaaagttg atttttcagg
    12121 cattaatgaa ccatggcttg atatgactaa atcaggaacg tcattaatgt ccaaagctgc
    12181 aaatacaatg cttcaatcaa aattaagtga attcttcact catggcaatt ttgatggtgc
    12241 agtaaatgcc aatggtgttt atcaatattt agttgatgtg gcacagaaag taatgggtaa
    12301 gttccccgga cttacagtaa cttctggata tcgagcagga gatgcctact atcacggaaa
    12361 acggcaagct atcgacttgg cttatccagg tatttctgga gatccgagat atacagcagc
    12421 agcaaattac gcttttgaaa aattcccttc aaaaattgcg tatgtcatta cgaatggacg
    12481 tgtacgtgac cgcatgggat tatctggaac aaggtcaagc ggacaatgga cgaattggcc
    12541 agatggcgat cacttcgatc atattcattt aaacggttcg atggggtctg gcgatatatt
    12601 caatggagga gttggaggaa gtggtgttgc acgctggcgt tcttatgtgt ctaaagcatt
    12661 gaaaatgaat ggattaccag ctacagcagc gtatataaat gcctggatgt cccaaatcca
    12721 aacggaatct ggtggtaatg agaaagcaat cggcggcaat gatgggctcg ccgaaggcaa
    12781 tgcaacagga ttacttcaaa cgaaacccgg aacttttgca gctaacgctt ttcctggtca
    12841 cggaaatatt atgaacggat ttgataacat gttagcagca atcaattatg ctaagaaacg
    12901 ttacggagtt gcaggtatgt tgcaagttat cgggaaagga catggatatg ccaatggcgg
    12961 cttgattacc aaagacggac tatatcgagc aggagaagga aataaaccag aaatggttat
    13021 tccattgaca agaaaaacaa gagctatcga acttatgggt caagcattag ccttcctttc
    13081 tggaagcaat aaaaaagaaa cgaaaagtga gattaattct gtagataata gacatatcct
    13141 acaagcgata gaaaaacagt ctcgtatgac aaatgatctg ctatcattat tactctcgtt
    13201 ttttaaaagt aacaatcgat cagataaaga actagcttta gatattcaga aaattttaat
    13261 caggaggaca tagtatttga ggacagtaat attaagaaat aaattgaatg aagaaattga
    13321 tttgtccaca gaagattatt ttgctactga aatgtcgaat ctaggttttg aagttacaaa
    13381 agagcatatt ggacaatggg ggagtttcag agaaatagga gagagtattg aattgtctga
    13441 atttcaatca tcagttatga tttctgttca cggatttaga gaacaacaat tatatggttc
    13501 attagttgaa tttctggcta atggaccttt tgaactagaa ttttcttttg attcagaaac
    13561 aatgatcagg aaatgtagtt tgaaatcatt atctaaaaca gaaattgatt cccaaagctc
    13621 attgctaaca gatactttga atctatattt tacttcaagc tggtattcta ttaagcgtga
    13681 aaaattaata cagaattcaa cttctctcaa tacacgtgga aaagtttact catttgttcg
    13741 accatatgtc tacaccaaaa atgtatggga aaagaaaggt gtatttaagt tcagtaataa
    13801 ttctgtatat ttgacaaata gtacagagcg aatgtctccg ttaaaaataa ggattattgg
    13861 agagtgctca aatccatatt gggaagttat ccaaaactct aaagtagtag cttcagatgg
    13921 ctattttatt aatatgaaag acacacaaat attagaagtg tctagtttgc tagaagacca
    13981 aacggcaatt ttaaaagatg taggtggagt tgaatcttct gtttatcaac aacaagattt
    14041 tactaaatca aacttcgtcc aagcgcctct aggagaattc agcgttgttt ttcatgttgg
    14101 acatgcaaat gtagagattg aactttatga ggagcgtgat ctattttgat tttagccatt
    14161 accctctttc accgagatat gaatttatat aacgaacgtt taatttcgac tgagttcgaa
    14221 tttggaacgg atgaaattgg agaagaagta agtaatttta ttacagataa atatgtccat
    14281 gtaaaatcag gagattttct actagcaaaa tttattccta gcgggaagat tgcttatttt
    14341 ggagtgatta gctcgcaaga agacaaaaaa ataaattgca aaggattact cagtttggcc
    14401 gatagcgaga taccgactgt aaaaatatca ggtgctaatt atgaagagca tattcgacga
    14461 ctgattgaat actacttatt gaacgatcca acaaaacaac taaaaaatat tttggaggta
    14521 aaaacagaaa gcgttacttc tcattcttat caagctgttg acacaaataa gagacgattg
    14581 aatgcatata taataaatgg gttcaaaaaa tataatgtta aatggtatgt caaagagatt
    14641 aaaggtaaaa aaatatatac tggtattcga gcagttgatg attcaattta tattaaagat
    14701 aattcatctg agtttaatga ctgggatgtt tttgtgcaat ctcctggacc agggaacgag
    14761 aacaaattat tgatcgtaga taaagcaatg aaagatatag aaaatccctt ggtattgtct
    14821 acgtggtatt tagatgacga aaataatctt acgcaagatt caacgaataa gaacattgca
    14881 ttgcctaccg ttaatttagt aaatatatat gatcaaactc aagaagatag accttcttat
    14941 gaagaggtag ctaaatcgga gcttaaaggc aacgcctatt cacatgaaat taaagttaat
    15001 gttgtgctaa atgctagaaa tatcaatgta gaaaatatcg aaacaggaat gttagcgact
    15061 attgtttaca aagagcagac ttacaaatct gttctaactg cctggcgaat atccagcagt
    15121 aaaaagaata tagaattgac atttggtaat gtccgaagtc gttttatgga ttattttgaa
    15181 gataatgggg gataaaaaat ggttagcaat gtggatggat atcaatttga aaacgtgaaa
    15241 gtaagcgcag aaaatgatgc tagactttat cacgttttat ataatcggaa aaatcaggtt
    15301 attgatggtt acgatcagtc tatgaattta tcttcaagcg gattaacagt aaaagttgct
    15361 gcaggagcag cgattattca aggtcgtatg gtcgttgttc gacaagaaga aagtataaca
    15421 gttccagcaa actcaagtgg ttatatagca ttaacagtgg atttgacaca agaagttata
    15481 cctggatcta ttcttccaga atcggaagaa tatgaatgga ctaataatca agtcaagcta
    15541 gaatttataa caaaagttat aaaaggtaat ttaaacaatg gtgataaggt ctataattta
    15601 ccactatgct cagttaattc tactggatca actgtttcaa tctcaaagat atcggatagt
    15661 tacgagctga ctctctctaa aggagaaatt ttgtggaggg ggactgcgtt aatgcatgat
    15721 actcaaactg tccaaccttc aaaaaaaatt tggcagacag tttcaggttt tttgttagta
    15781 tggctcccat acgaaaacgg gcaagcaatt gaggatagat atgtaactac gcctttttat
    15841 aaggagcgta taacttttac taatgtttta ggagaaattg tttcaggatt tgatgactat
    15901 cacaaaaagt ggtttagtaa acgaataaac tataattcga atactaatat atttacaggt
    15961 gcagcaagca atgcaagtgg agataacgca aatatggtgc ttaggtatat agtttctttt
    16021 tagttaggag gtatggaaga gtggcaaatt tagaaattaa attatctgca aataagagac
    16081 agccttatct acgtcaccgt gttgttggta gagttggtga tggggggctc acaacaatca
    16141 atgtacaact tcttgaggaa gatgaaatta caccttttgt aattaatcta aacggtactt
    16201 tgaaatttgt gggcgaagtt tcaaacggta actataccga gggagaacca gaaataatcg
    16261 attcgactaa tgggttaatt agttacacgt tcactaagtc aaatttcagc acgagtcatc
    16321 aattcaaaca agcatatttt gaatatgtag atcctaacgg caaaaaagta acttttcaga
    16381 acttcatcat agacgtgtta gaacgagtag atattaattc ggagcaagcg aaatactata
    16441 tttcttcatt ggaaaaatta cagagtgaaa tgcaaaccac tttcaatcag ttcattagtg
    16501 ataaacaggt ccaatacgat caaatctact cgaaatataa cgaattagta agattgataa
    16561 atgaatcaga taagcaagta aatgatcgta ttgataaggc aaataatcgt attgatcaaa
    16621 ccaatcagca aatcggcgat ctcggcaagc tgaaaaagat gtacagtaac agcattgatt
    16681 tcgggggata tgattatagc ggtaagccga atttaatgtc caaactaaaa tcgagcgatt
    16741 ttaacgttgg ttaccacggg tcactaactt cggataacga aaagctacat tttacttctg
    16801 atggtacagg aagcattatt atgtttacgc gtattaatac acctcagctt gctagtggga
    16861 aaacctatac tctgagtgcg aaagttcgat ttgatgaagg aactacagga gctattgata
    16921 aattacgttt ggtgtatcgt acatcaccag gagaaaagat attattggaa gcaaatagta
    16981 caaatattac aacagatgat gtagggaaag aaataacaat caaaggtaca gctaacgtta
    17041 attatcaaat cacaaattta gatcgatttt atatgagtat tagctttgtt gacagggata
    17101 aaataaatgg cggatttaag ttgtacgaca tcaaaatcga agaaggctca acagccacac
    17161 cgtaccagcc taacttacta gatgaccctt actgggtagg taaaacatcg ttgggtgaga
    17221 atattgcaaa taaaggtacg acgttcccaa ttaaatctag cgcgtacgaa atatataaag
    17281 gtaacacgga agaagagctt ataataggtc aaacgtatac tatcacgctt aaaggaacaa
    17341 aacccgcaag tcaaaccttt gtagcatata attattggaa cgttaatttt ggagaactaa
    17401 aaccagttga gggattgaca gacgtatggt ctctaacatt cacaccaacg aaacttgaac
    17461 cgggtttgcc taaagacctt cgcatttttc agttgcctaa agaaacggcc ggcgcatgcc
    17521 aaattgactg gctcaaaatt gaaaaaggcg acacccgaac cccgaatatt agtcagttta
    17581 aatactttgg tgaaggattg aaagatagca acaatccgaa cgactacagc tgggacatca
    17641 cgcctgaata tactgaaaaa ggcttgaatg atacggttag tttgactgaa ccgcaatcca
    17701 tcgaaggatt gaaaaatttc gaagatggta ttcaatcaaa aggaaaatct gtattgacat
    17761 cagacgacaa caaatatgaa gtcgtaacct taacagttac aaacgggaat accggatcag
    17821 caaagcttta tcgtgaagga aaaaccgtca ctatttattt ttttgcgttg aatgggaaaa
    17881 gcagtggtgg aaatgattca acgatactaa caattccaga aggctatcgg ccaccaatta
    17941 gttttgagca actggttggc tcgatagacc gttctacttt gaacagtgct cagttatcta
    18001 ttggtgcaga tggagccatt aaatggcgaa gaaactcaag ttatggatca gcttattcat
    18061 ttgttatcac ttattcaatt taagggagga aatctaatga aagtagttta caaatcaatc
    18121 aagccttacg gattcgagca aatcattgtg aatgacgaag aacatttacc ggaagaatgc
    18181 acagaagtcg aaccaccgat tccaaattgg aaaccgaaat tcaattactg ggagggaaat
    18241 aaatgaaaaa catttggaaa tatggccgta ctggcggaga gtatgcagga aaagtattgg
    18301 acgacatgct tgtatccgtt ccttacacag atcagccacc gctcgaaggg gttcgttcag
    18361 atggcgaacc gctaacgatt gctgatcaga tgtttgatcc taaattgaac caatggatta
    18421 ttttagcgaa cgcgttagat cacaacgatt taaacaatct caaagcgatg tacgaggctc
    18481 ttgaacatga aaacggcaac ctaaaacagc taaatgccaa actcatgcta agcgatgtag
    18541 cgattaaaca ggaaaatact gcattgaaag aaaaagctga cagtttagca caaatcaatt
    18601 caaaaatgat gcttgcttcg ttacaaaata gcaaagacat ttcagaaatt aaagagcaac
    18661 taaatccagc ttcaaaagga ggtgagtagt atgtttagtt ttagcgatgt gaaaatgatg
    18721 tatgattggg gctgttttac tgacgatcaa gttcgactat tcgttccact atgcattaca
    18781 gacgaagaag cagataaaat cattaataaa gataagagcg catcttaatt gatgcgtttt
    18841 tatttaaggt aaaggagttg tcacatgatt aatttagggg aatggggaat gatagcagga
    18901 tcaataaccg ctatcgtttc tttgatttta ttagtaatta gaccgattgc tgcatctttc
    18961 tcgaaaatta ctgagactct ttcaaaagta agccacaatt tagatttgct gactaaagat
    19021 ttagaatcga gcaaatcaga tcgattgatg attcatgaag aactaaagaa acacgatgaa
    19081 agattagata cacatacaga aaaattggtg gaacacacgc aacaaatcaa aactttgttt
    19141 agggagagaa gaaaatgaat aataaaacgt tcgaagtact aaaatggttc gcactggtaa
    19201 ttattcccgc actagctact ttcgtggggt tagttggtaa agcgctcaat tggcagtaca
    19261 cagatatctg tgttgtcatc attactggtt ttggcgcgtt tttagggagt gtgttgggtg
    19321 tatcaaatcg aacctacaaa atgttctcgg ctgaaagcga agaaggagga aacaaatgaa
    19381 aaagaaaatt actattactg cgatgagcct gttaacggct ctttttttat tgccaattaa
    19441 cggatttgcc tatactatca acaatgaatt taatttgggc gcaaatgaag gtagctcaca
    19501 agtagcaaat aatcagtata ttttactgca tgaaacggct aatgaaacag caacaggacg
    19561 caatgaagcg cagtatatgc aacgttcatg gactagcgct tatactgctt atattgtggg
    19621 agacggcgga attgtttacc aagtcggaca acctggttat gtacagtacg gtgctggttc
    19681 atatgctaat gccaatagtc ctgtgcagat tgagttacaa cacacacatg ataaagcaac
    19741 ttttgaaaaa aactataaag catacgttga attggctaga gattcagcaa tgaaatatgg
    19801 tattccatta acattagaca cgccttataa ccaaccagga atcaaatcac atttatgggt
    19861 aactcaaaac atttggggcg atcacactga tccgtatgga tacttatcag aaatgggtgt
    19921 aagtaaggaa aaactggctt acgatttagc tcatggattt acagacgaaa acccaacgac
    19981 ttctgagaac aagcctgtca ttgatccaac ccgagcaggt gcagcaaatc caactttatc
    20041 agatggaacg aaccattctc acattgatca gtttggagaa atcgaaaatg cgaacttaca
    20101 cgtcgctgga tggcacatcg ctaactataa gtacgagtat attttcatta tggactacaa
    20161 tactgggaaa gaattagctc gagtaagagc tgatggaatt tatagaccgg atgtaaatca
    20221 agcttataat acttctggaa atgttggcta tcatgtatct tttaacatgc gtaacttccc
    20281 taataagaaa gtctatgtca tgatgcgggc aacgaatgat ccagagggaa acactaaagg
    20341 cggtgcgcaa gatttccatg acaaacgttg gtatttaaat attcctaaac gataaaaata
    20401 gctcctcgtt gaggagcatt acataactat attgacaact ataaaaatca ttcgataaaa
    20461 tagtgatgtt atcgcatatc ttcactatca cccataatag tcacactcca agctatgcga
    20521 taacaggttt gttgccacac attctactgg ttgattgttt atggctttat gtggcaacaa
    20581 ccagtaccct tagctcagtt ggtcagagca gacggctcat aaccgtccgg tcgtaggttc
    20641 gagtcctaca gggtacatta acgtagccat ttgaatcgtt ctgtgttaga attttttgaa
    20701 gagtattata caagctaaag cttttcttca ttgccactca aatgagtggc ttttttatgt
    20761 atccttttat ggattaatga aaggatgttt cacatagtta tatttctgta tatttgaaaa
    20821 gttttacttt gatttttaaa tagaaagaca tttgggttat attgtgagat aataataaag
    20881 aagagtttaa agcgttcccc aaaaaccact gccccataag tgtgttacgc tttaaactct
    20941 tttatatttg aagccattaa aaagcatacc atatttttga aaaaaagtga gaaaaaaggc
    21001 ttataattgg agtggtag
//
LOCUS       DACDMM010000035        20907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDMM010000035 DACDMM010000000
VERSION     DACDMM010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.035"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IWD16_000989"
     CDS             591..1280
                     /locus_tag="IWD16_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9088069.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IWD16_000990"
     CDS             1267..2475
                     /locus_tag="IWD16_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9088070.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IWD16_000991"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IWD16_000991"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP9088071.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWD16_000992"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWD16_000992"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP9088072.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IWD16_000993"
     CDS             complement(6578..7273)
                     /locus_tag="IWD16_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP9088073.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWD16_000994"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWD16_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP9088074.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IWD16_000995"
     CDS             complement(8472..8663)
                     /locus_tag="IWD16_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP9088075.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IWD16_000996"
     CDS             complement(8684..9574)
                     /locus_tag="IWD16_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP9088076.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IWD16_000997"
     CDS             complement(9561..10328)
                     /locus_tag="IWD16_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9088077.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWD16_000998"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWD16_000998"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP9088078.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWD16_000999"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWD16_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP9088079.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWD16_001000"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWD16_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP9088080.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IWD16_001001"
     CDS             14944..15282
                     /locus_tag="IWD16_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088081.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IWD16_001002"
     CDS             complement(15450..16208)
                     /locus_tag="IWD16_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9088082.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IWD16_001003"
     CDS             complement(16226..17044)
                     /locus_tag="IWD16_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9088083.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWD16_001004"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWD16_001004"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP9088084.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWD16_001005"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWD16_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP9088085.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IWD16_001006"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IWD16_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP9088086.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IWD16_001007"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IWD16_001007"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP9088087.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5980 a         4257 c         3833 g         6837 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccct
//
LOCUS       DACDMM010000036        20675 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDMM010000036 DACDMM010000000
VERSION     DACDMM010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.036"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            235..543
                     /gene="rpsJ"
                     /locus_tag="IWD16_001008"
     CDS             235..543
                     /gene="rpsJ"
                     /locus_tag="IWD16_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP9088088.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            574..1203
                     /gene="rplC"
                     /locus_tag="IWD16_001009"
     CDS             574..1203
                     /gene="rplC"
                     /locus_tag="IWD16_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP9088089.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1231..1854
                     /gene="rplD"
                     /locus_tag="IWD16_001010"
     CDS             1231..1854
                     /gene="rplD"
                     /locus_tag="IWD16_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP9088090.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1854..2144
                     /gene="rplW"
                     /locus_tag="IWD16_001011"
     CDS             1854..2144
                     /gene="rplW"
                     /locus_tag="IWD16_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP9088091.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2183..3016
                     /gene="rplB"
                     /locus_tag="IWD16_001012"
     CDS             2183..3016
                     /gene="rplB"
                     /locus_tag="IWD16_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP9088092.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3059..3337
                     /gene="rpsS"
                     /locus_tag="IWD16_001013"
     CDS             3059..3337
                     /gene="rpsS"
                     /locus_tag="IWD16_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP9088093.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3360..3707
                     /gene="rplV"
                     /locus_tag="IWD16_001014"
     CDS             3360..3707
                     /gene="rplV"
                     /locus_tag="IWD16_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP9088094.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3721..4377
                     /gene="rpsC"
                     /locus_tag="IWD16_001015"
     CDS             3721..4377
                     /gene="rpsC"
                     /locus_tag="IWD16_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP9088095.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4380..4814
                     /gene="rplP"
                     /locus_tag="IWD16_001016"
     CDS             4380..4814
                     /gene="rplP"
                     /locus_tag="IWD16_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP9088096.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4804..4992
                     /gene="rpmC"
                     /locus_tag="IWD16_001017"
     CDS             4804..4992
                     /gene="rpmC"
                     /locus_tag="IWD16_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP9088097.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            5017..5283
                     /gene="rpsQ"
                     /locus_tag="IWD16_001018"
     CDS             5017..5283
                     /gene="rpsQ"
                     /locus_tag="IWD16_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP9088098.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5341..5709
                     /gene="rplN"
                     /locus_tag="IWD16_001019"
     CDS             5341..5709
                     /gene="rplN"
                     /locus_tag="IWD16_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP9088099.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5746..6054
                     /gene="rplX"
                     /locus_tag="IWD16_001020"
     CDS             5746..6054
                     /gene="rplX"
                     /locus_tag="IWD16_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP9088100.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6082..6621
                     /gene="rplE"
                     /locus_tag="IWD16_001021"
     CDS             6082..6621
                     /gene="rplE"
                     /locus_tag="IWD16_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP9088101.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6640..6825
                     /locus_tag="IWD16_001022"
     CDS             6640..6825
                     /locus_tag="IWD16_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP9088102.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6861..7259
                     /gene="rpsH"
                     /locus_tag="IWD16_001023"
     CDS             6861..7259
                     /gene="rpsH"
                     /locus_tag="IWD16_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP9088103.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7291..7827
                     /gene="rplF"
                     /locus_tag="IWD16_001024"
     CDS             7291..7827
                     /gene="rplF"
                     /locus_tag="IWD16_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP9088104.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            7988..8344
                     /gene="rplR"
                     /locus_tag="IWD16_001025"
     CDS             7988..8344
                     /gene="rplR"
                     /locus_tag="IWD16_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP9088105.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8365..8865
                     /gene="rpsE"
                     /locus_tag="IWD16_001026"
     CDS             8365..8865
                     /gene="rpsE"
                     /locus_tag="IWD16_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP9088106.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8880..9059
                     /gene="rpmD"
                     /locus_tag="IWD16_001027"
     CDS             8880..9059
                     /gene="rpmD"
                     /locus_tag="IWD16_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP9088107.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9103..9543
                     /gene="rplO"
                     /locus_tag="IWD16_001028"
     CDS             9103..9543
                     /gene="rplO"
                     /locus_tag="IWD16_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP9088108.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9543..10838
                     /gene="secY"
                     /locus_tag="IWD16_001029"
     CDS             9543..10838
                     /gene="secY"
                     /locus_tag="IWD16_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP9088109.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10900..11547
                     /locus_tag="IWD16_001030"
     CDS             10900..11547
                     /locus_tag="IWD16_001030"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP9088110.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11738..11956
                     /gene="infA"
                     /locus_tag="IWD16_001031"
     CDS             11738..11956
                     /gene="infA"
                     /locus_tag="IWD16_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP9088111.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            11989..12105
                     /gene="rpmJ"
                     /locus_tag="IWD16_001032"
     CDS             11989..12105
                     /gene="rpmJ"
                     /locus_tag="IWD16_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP9088112.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12123..12488
                     /gene="rpsM"
                     /locus_tag="IWD16_001033"
     CDS             12123..12488
                     /gene="rpsM"
                     /locus_tag="IWD16_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP9088113.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12516..12905
                     /gene="rpsK"
                     /locus_tag="IWD16_001034"
     CDS             12516..12905
                     /gene="rpsK"
                     /locus_tag="IWD16_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP9088114.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            12985..13923
                     /locus_tag="IWD16_001035"
     CDS             12985..13923
                     /locus_tag="IWD16_001035"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP9088115.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            13951..14331
                     /gene="rplQ"
                     /locus_tag="IWD16_001036"
     CDS             13951..14331
                     /gene="rplQ"
                     /locus_tag="IWD16_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP9088116.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14603..15442
                     /locus_tag="IWD16_001037"
     CDS             14603..15442
                     /locus_tag="IWD16_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9088117.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15418..16287
                     /locus_tag="IWD16_001038"
     CDS             15418..16287
                     /locus_tag="IWD16_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9088118.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16277..17074
                     /locus_tag="IWD16_001039"
     CDS             16277..17074
                     /locus_tag="IWD16_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9088119.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17277..18023
                     /gene="truA"
                     /locus_tag="IWD16_001040"
     CDS             17277..18023
                     /gene="truA"
                     /locus_tag="IWD16_001040"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9088120.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(18356..>20675)
                     /locus_tag="IWD16_001041"
     CDS             complement(18356..>20675)
                     /locus_tag="IWD16_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9088121.1"
                     /translation="VGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIMW
                     LDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLLT
                     NWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDLI
                     MKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASFY
                     LVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAAL
                     FAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMSA
                     EALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSAN
                     DSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYNI
                     SKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNLI
                     NTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQA
                     YLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGTK
                     EISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYDK
                     GWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFILY
                     VWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         6851 a         3785 c         4480 g         5559 t
ORIGIN      
        1 ctttatgaaa acaaaatgtt ttttacaaga aaaagccttg ctttgtgagt ttatatatat
       61 tataatgaca aaggtgcgtt tactttagtg aattgcacag acgtaatcag aatgatttcg
      121 tcggctaaga aacgagaggt tgcgacacgc ccggacgctt tgccacgaac gagcgtgacg
      181 gaaattttcg tggagctatg tctacttttc aaaataggcg aaggagggaa caagatggca
      241 aaacaaaaaa ttcgtatccg tttaaaagcg tatgaacacc gtattttaga tcaatcagcg
      301 gataaaatcg tggaaactgc aaaaagaact ggagctagcg tgtctggtcc aattccatta
      361 ccaactgaac gcagtcttta cacaatcatc cgtgcgactc ataaatacaa agattcacgc
      421 gaacaattcg aaatgcgtac acacaaacgt cttatcgaca ttgtgaaccc aacaccaaaa
      481 actgttgatg ctttgatgaa gctagactta ccatctggtg taaacatcga aatcaaatta
      541 taaaactaaa acaaaaataa tggaggtgta ctcatgacca aaggaatctt agggaaaaaa
      601 gtgggaatga cacaaatctt cactgaatct ggtgaattaa ttccagtaac tgttgtagaa
      661 gctactccaa acgtagtact acaagtgaaa acaatggaaa cggatggcta cgaagctatt
      721 caagttggtt accaagatat gcgtgaagtt ttatcaaaca aacctgcgaa aggtcatgtt
      781 gcaaaagcaa acacggctcc taagcgcttc attcgtgaat tcaaaaatgt tgagctagga
      841 gaatatgaag taggaaaaga aattacagta gatgttttcc aagcaggaga cattattgat
      901 gtaacaggta ctacgaaagg taaaggattc caaggggtta tcaaacgcca cggacaaagc
      961 cgcggaccaa tggctcacgg ttctcgttac catcgtcgtc ctgggtcaat gggtccagtt
     1021 gcacctaacc gtgtatttaa aaacaaacgt cttgctggcc gtatgggtgg caaccgcgta
     1081 actatccaaa acttggaagt tgtacgtgtc gatgcagaaa gaaatgttat cttgatcaaa
     1141 ggtaatgttc ctggagctaa aaaatcttta attactatca aatcagctgt gaaagctaaa
     1201 taattgtagg aagaaaggag gaactaagga atgccgaatg tagcattatt caaacaagat
     1261 ggaagccaaa acggtgaaat cacacttaac gaagaaatct tcggaattga acctaatgaa
     1321 agtgttgttt acgatgcaat cgtaatgcaa cgtgcgtcat taagacaagg aacacacgcg
     1381 gttaaaaacc gtagcgctgt tcgcggcggc ggacgtaaac catggcgtca aaaaggaaca
     1441 ggtcgtgcac gtcaaggatc tatccgctca ccacaatggc gtggaggtgg cgtagtcttt
     1501 ggaccaacac cacgttctta cagctacaaa cttcctaaaa aagttcgtcg tttagcaatg
     1561 aaatctgtat tgtcagaaaa agttgctgaa aacaacttgg tagcaatcga aggcttgaac
     1621 ttcgacgcac caaaaacaaa agaattcaaa caagttcttg ctaacttatc tatcgactct
     1681 aaagtattgg ttgttctaga aaaaggtaac gactttgctg ctttatcagc acgtaactta
     1741 ccaaacgttt ctgtagtaac ttctgataat gtgagtgtgc tagacgttgt atcaaataca
     1801 aaagtgttag caacacaaac tgctcttact caaattgagg aggtgcttgc ataatgaact
     1861 tactagacgt aatcaaacgc ccagtgatca ctgaaaaatc aatgcttgcc atggatgaca
     1921 agaaatacac ttttgaagtg gacactcgcg caaacaaaac tttagtaaaa caagcggtag
     1981 aagctgcttt tgacgttaaa gtcaaaaacg tgaacatcgt gaacgtacgt gcgaaattca
     2041 aacgtatggg caaatatgca ggatatacaa aaaaacgtcg caaagcgatc gttacattga
     2101 ctgaagattc aaaagaaatc caattattcg aagctgctga ataagcacta atagataact
     2161 cattaaatta ggagggaaaa acgtggcgat taaaaagtac aaacctacct caaatggtcg
     2221 tcgtaacatg acaggttctg attttgctga aatcacaaca tcaacacctg aaaaatcatt
     2281 gttacaacca ttgaaaaacc atgctggacg taacaacaac ggccgcatca ctgtacgtca
     2341 tcaaggtggc ggacacaaac gccaatatcg cgtgatcgac ttcaaacgta acaaagataa
     2401 cgttgttgca actgttcaaa cgatcgaata tgatccaaac cgttcagcta acatcgcgtt
     2461 agtacactat gaagatggag tgaaagctta catcttagca cctaaaggac tagaagtggg
     2521 tatgaaactt gtttcaggtc cagaagcaga tatcaaagtc ggaaatgctc ttccattaga
     2581 aaacattcca gttggtactg ttatccacaa cattgaaatg aaacctggta aaggtggaca
     2641 attgatccgt tctgctggta caagtgctca agtacttggt aaagaaggca aatacgtatt
     2701 gatccgcttg aactctggtg aagttcgtat gatcttagct gcatgccgtg ctacaatcgg
     2761 ttctgtaggt aacgaacaac acgaattaat caacatcggt aaagctggac gctctcgttg
     2821 gatgcgtaaa cgcccaactg tacgtggtag cgtaatgaac cctaatgatc acccacacgg
     2881 tggtggtgaa ggtaaagctc ctatcggacg taaagcacca gtttctcctt ggggtcaacc
     2941 agctcttggt tacaaaactc gtaacaaaaa agctaaatca gacaaactta ttgttcgtcg
     3001 tcgcaaaaat aaataattac tttgaccatg tgtcgcacat tttgagagga ggttcaccat
     3061 gggtcgtagt ttaaagaaag gaccttttgt cgatgagcat ttaatgaaaa aagttgaagc
     3121 tcaagcaggg gttgaaaaga aaaaagttat caaaacttgg tcacgccgtt caacagtttt
     3181 cccaagtttt gttggatata ccatcgcagt ttatgatgga cgtaaacatg taccagttta
     3241 catccaagaa gacatggtag gacacaaatt aggtgaattt gcacctacaa gaacttatcg
     3301 tggacatgct gctgacgata agaaaacgaa acgctaattt gaggggagga taaatccaaa
     3361 tggcagaaca aatcacatca gctaaagcaa ctgcaaaaac agttcgcact tcacctcgaa
     3421 aagcccgttt agtaattgac cttatcagag gtaaaagtgt cgcagatgca atttcaatct
     3481 tgaaattcac accaaacaaa tctgctggaa tcattgaaaa agtattgatg tcagcagttg
     3541 cgaatgcaga aaataacttt gacttagatg ttgaaaactt ggtagtaagc gaagcttttg
     3601 ttaacgaagg acctacaatg aaacgtttcc gtcctcgtgc aaaaggatca gcttctccaa
     3661 tcaacaaacg tacaagtcat atcacggtag tcgtatcaga aaaataagga gggataatca
     3721 gtgggtcaaa aagtacatcc aattggaatg cgtgtaggca tcatccgcga ctgggatgct
     3781 aaatggtatg ctgaaaaaga gtatgctgag ttcttacacg aagatttaag aatccgtaaa
     3841 tttatttcaa ctaaacttgc tgatgctgct gtatcaacag ttgaaatcga acgcgcaaca
     3901 ggtcgcgtaa acatttcaat tcacacagct aaaccaggta tggttattgg taaaggcggt
     3961 tctgaagtcg aaagcctaag aaaagaatta aacaaattaa ctggtaaaag aatccatatc
     4021 aatatcgtgg aaatcaaaaa accagatcta gatgcaaaat tagtaggtga aggaatcgca
     4081 cgtcaattag aaaaccgtgt tgctttccgt cgcgctcaaa aacaagccat ccaacgctca
     4141 atgcgtgctg gcgctcaagg gatcaaaact caagtatctg gtcgtctaaa cggagcagat
     4201 atcgctcgtt cagaaggata ctcagaagga actgttcctc ttcatacttt acgtgcggac
     4261 attgattacg catgggaaga agcagataca acatacggaa aactaggagt taaagtgtgg
     4321 atctatcgtg gagaaattct tccaactaaa aaaaacactg agaaaggagg gaaataatca
     4381 tgttagtacc taaacgtgta aaacaccgtc gtgaattccg cggaaaaatg cgcggggaag
     4441 ctaaaggcgg aaaagaagta gcattcggtg aatacggttt gcaagctgtt gattcacatt
     4501 ggatcacaaa ccgccaaatc gaagctgctc gtatcgcaat gactcgttac atgaaacgtg
     4561 gtgggaaagt atggattaaa attttccctc acaaatctta tactgccaaa gcaattgggg
     4621 tacgtatggg ttctggtaaa ggggcacctg aaggatgggt tgcaccagta aaacgtggta
     4681 aaatcatgtt tgaaatcgca ggcgttcctg aagaagtagc tcgtgaagcg ttacgtctag
     4741 cttctcacaa attaccaatg aaaactaaga tcgtaaaacg tgaggaaatg ggtggtgaat
     4801 cgaatgaagg ttaaagaaat cagagaatta accactgccg aaatgcttga tcaagaaaaa
     4861 caattaaaag aagaattgtt taatcttaga ttccaattag caacaggtca attagaaaac
     4921 actgcacgta ttaaagaagt acgtaaatcg attgcacgca tcaaaactgt gttgcgtgaa
     4981 caagctaagt aattatggaa ggaggccatt aagcgtatga ctgaaggaag aaatcaacgc
     5041 aaagtttatc aaggtcgcgt ggtttcagac aaaatggata aaacaattac cgttgctgtc
     5101 gaaacaaaga aaaaccaccc tatctatggc aaacgcatga actattctaa gaaatacaaa
     5161 gtgcatgatg aaaacaacac agcaaaagtt ggcgacatcg tgagaattat ggaaactcgt
     5221 ccattatcag ctacaaaacg tttccgtttg ttggaagtag tcgaagaagc agttattatc
     5281 taataacacg agattttcct atatagattg aatgcaaaat ctgaaaggag gatacacaac
     5341 gtgatccaag cagaaagtcg tttaaaagtc gctgataact caggcgcacg tgaaattttg
     5401 acgatcaaag tccttggtgg atctggtcgt aaaactgcta acatcggtga tgtgatcgtt
     5461 gctacggtta aacaagcaac gccaggtggg gttgtcaaaa aaggtgacgt cgttaaagcc
     5521 gttatcgttc gaacaaaatc aggagctcgt cgtacagacg gttcatatat taaatttgat
     5581 gaaaatgctg cagtgattat tcgtgatgat aaaagccctc gtggaacacg tatctttggg
     5641 ccagttgcac gtgaactacg tgaaaacaac ttcatgaaga tcgtttcact agcaccagaa
     5701 gttttataat cttgagacac ttatcaaagg aggtgcgaaa cagttatgtt tgttaaaaaa
     5761 ggcgataaag ttaaagttat cactggaaaa gacaaaaaca aagaaggcgt tgtactagca
     5821 gcgttcccga aaaaagataa agttattgtc gaaggtgtaa atgtcgttaa aaaacaccaa
     5881 aaaccttctc aagctgctcc tcaaggtgga attgttgaga tggaagcgcc aattcatgtt
     5941 tctaacgtga tggtggttga ctctactggt gtagctggcc gagttggcta caaagaagta
     6001 gacggcaaaa aagtccgtgt ttctaaaaaa accggtgaag ttttagataa ataaaagttt
     6061 aaggaaggag ggtcttactg aatgaaccgc ctaaaagaaa aatatcttaa agaagtaact
     6121 ccatcattga tggaaaaatt taactatagc tctgttatgc aaacacctaa agttgaaaaa
     6181 atcgtcatca acatgggtgt tggtgatgcg gtatcaaacg ctaaaaactt agataaagca
     6241 gttgaagaat taactttgat cactggtcaa aaaccaatga ttacaaaagc taaaaaatca
     6301 atcgctggat tccgtttacg tgaaggaatg ccaattggtg caaaagttac attgcgcgga
     6361 gaaagaatgt acgaattctt agacaaattg gtttctgttt cactaccacg tgtacgtgac
     6421 ttccatggtg taagtaaaaa agcctttgac ggtcgtggaa actatacttt aggtattaaa
     6481 gaacaattga tcttcccaga agtagattat gatttagtag ataaagtacg tggtatggac
     6541 atcgttattg taacaacagc aaactctgat gaagaatctc gtgagttgtt gacacaatta
     6601 ggcatgccat tccaaaaata attaaaggag gcgaactacg tggctaaaaa atcaatgatt
     6661 gctaaaaaca aacgtcctgc aaaattttca acacaagctt atactcgttg cgaacgttgc
     6721 ggacgtccac attcagttta tcgtaagttc catctttgcc gtatttgctt ccgcgaactt
     6781 gcctataaag gtcaaattcc cggcgtgaag aaagctagct ggtaaaaagt aaattcgcat
     6841 aagaaggagg taaaagatca atggtcatga cagatccaat tgcagatttt ctaactcgta
     6901 tccgtaacgc aaacatggtt aaacatgagt ctttagaagt acctgcatca aaaattaaac
     6961 gtgacatcgc tgaaatcttg aaacgtgaag gtttcgttcg tgatgttgaa tacatcgaag
     7021 atgacaaaca aggcgtgatc cgtgttttcc taaaatttgg taaaaacggc gaacgtgtta
     7081 tcacaaactt aaaacgtatc tctaaaccag gtttgcgtgc ttatgtcaaa gctgacgaag
     7141 taccaaaagt attgaacggt ttaggtatcg caatcatttc gacttctgaa ggtgttgtca
     7201 ctgataaaga agctcgtgaa aaaaatatcg gcggcgaagt tatcgcttac gtatggtaat
     7261 caagtagaat ctacaaggag gtgtctttaa gtgagccgta tcggaaataa agtcgttgtt
     7321 atccctgaag gagtaacagt tactcaagac ggaaacaacg ttacagttaa aggaccaaaa
     7381 ggtgaattga ctcgtacttt ttcagcagat atcaaaatga atatcgaagg aaacgaagta
     7441 actttcactc gtccaaacga cagtaaagaa atgaaaacaa ttcatggaac aactcgtgcg
     7501 aacttcaaca acatggttgt aggtgtcagc gaaggtttcc aaaaagcgtt agaactaatc
     7561 ggggttgggt accgtgcaca attacaagga tcaaaacttg tattgaacgt tggttactca
     7621 catccagtag aaatcacacc accagctgga gtaactgttg aagtaccagc taacacacaa
     7681 gtaattgtta aaggtgcgaa caaagaagaa gttggcgaat tagctgcaaa cattcgtgga
     7741 actcgtcctc cagaacctta taaaggtaaa ggtattcgtt atgttggcga atatgtacgc
     7801 cgtaaagaag gtaaaactgg taaataatcg tttagaaaaa ttgcatagaa gccagcagca
     7861 ggaaaaaggc atttggctga agtttacctt caaaaaaata catttttact tgtgccgcag
     7921 cttctatttt tcgaaacgta tttactgctt gaaatagtag tacaataaaa ataaagaggt
     7981 gacaattgtg attacaaaac cagataagaa caaaacacgt caaaagagac atcgtcgtgt
     8041 acgtaacaat atctctggta ctgctgagcg cccacgcttg aacgtttttc gttctaacaa
     8101 aaacatctac gctcaaatta ttgatgacgt agcgggtgta acgctagcaa gtgcctctac
     8161 cttggataaa gaaatttcag gtggaacaaa aacagaagca gcacaagctg tcggtaaact
     8221 agttgctgaa cgtgcagctg aaaaaggcat taaagtagta gtctttgacc gtggtggata
     8281 cctttaccat ggccgtgtgc aagctttagc tgaagctgct cgcgaaaatg gactagaatt
     8341 ttagaagaag gaggaatacc attcatggtt tatatcgatc caaaacattt ggaattagaa
     8401 gaccgcgttg ttgcaattaa ccgtgtaaca aaagttgtta aaggtggacg tcgtctacgt
     8461 tttgctgctt tagttgttgt cggtgacaaa aacggacacg tagggtttgg tacaggtaaa
     8521 gctcaagaag ttcctgaagc aatccgtaaa gcaattgaag atgcgaagaa aaacttagtt
     8581 gaagtaccta tggttggttc aacaatccca cacgaagtaa tcggtgcttt tagtggtggt
     8641 agaatcctaa tgaaacctgc tgtagaaggt tctggggttg ctgctggtgg accagttcgt
     8701 gccgtattag aattagctgg ggtagcagat atcacttcta aatctttagg ctcaaataca
     8761 ccaatcaacg ttgttcgcgc aacagttgaa ggtttgaaac aattgaaacg tgcagaagaa
     8821 gttgcagaac ttcgcggcaa atcagttgaa gaattaatcg gataaggagg acaacaataa
     8881 tggctgaatt aaaagttact ttaaaacgca gtattatcgg acgtcctcaa aaccaacgcg
     8941 atacagttaa agcgttaggt cttacaaaga ttaacagttc agtggttaag ccttctaatg
     9001 aagcaattaa aggcatgatc aatactgttt ctcacctagt ggacgttgaa gaaatttaat
     9061 ttagaaaaat cataactaga acattaagga ggtgccaaac ctatgaaact tcatgaatta
     9121 aaacctgctg aaggttcacg ccaagtacgt aaccgtgttg gtcgtggtac ttcatctggt
     9181 aacggaaaaa ctgccggacg cggacaaaaa ggacaaaaag ctcgttcagg cggcggtgtg
     9241 cgtttaggat ttgaaggggg acaaacacca ttgttccgtc gtttaccaaa acgaggattc
     9301 acaaacgtga accgtaaaga ctatgcagtc attaatcttg atgtattgaa ccgctttgaa
     9361 gacggagctg aagtaactcc tgttgcttta gttgaagctg gaatcgtgaa aaacgaaaaa
     9421 gctggaatca aagtattagc taatggggaa ctaaacaaga aattaactgt gaaagcagca
     9481 aaattctcta aagcagctca agaagcaatt gaagcggctg gtggatcaat cgaggtgatc
     9541 taatgtttag actattaaaa gatgctttta aagtaaaaga cattagatcg aaaatcctat
     9601 ttactgtatt tgtgttgttt gtatttcgcc taggtgcaca tattactgta ccaggcgtaa
     9661 atgctgcttc attgcaaaac ctttcttctt tgccattttt caacatgttg aacttggtaa
     9721 gtggtagtgc gatgcaaaac ttttccattt tctcaatggg ggtttcacca tacattactg
     9781 cttctatcgt cattcagctt cttcaaatgg acatcgtgcc tcgatttgtt gaatggtcga
     9841 aacaagggga agttggtcgt aaaaaactga atcaagcgac tcgatatctg acattagtat
     9901 tggcatttat ccaatctgtc ggtatcactg ctggattcaa tcaatacgct caattaggtt
     9961 tcgtgaataa tcctaacgta gcaacttacg taacgatcgg tttgattcta acagccggta
    10021 cgatgtttgt tacttggcta ggagaacaga tcacagaaaa aggtatcgga aatggcgtat
    10081 caatgatcat ttttgcaggt atcatttcta gattgcctga aagtatcaga gaaatttatg
    10141 aagattactt tataaatatt gatccttcag acatatggaa atctgctatt tttgtggcca
    10201 ttttactagt agcggtcttg ttgatcgtga cattcgtaac atttttccaa caagctgaac
    10261 gcaagatccc gatccaatat acgaaacgcg tagctggcgc gcctacaagc agctatctgc
    10321 cgttgaaagt aaatgcagct ggagttattc cagttatctt tgctagttcg tttattgcta
    10381 cgccaaatgc tgttttacaa gcattgagcg gtagattcgg ttctgaaaac tggtacaaag
    10441 tgatgatgga gatctttaac tataatacag cacctggtgc tacgatttat acgattctga
    10501 tcgttgcctt cactttcttc tacgcctttg ttcaagtaaa ccctgagaaa ttagcggaaa
    10561 acttgcaaaa gcaaggaagt tatataccaa gcgtacgccc tggtaagggg actgaagagt
    10621 atgtctcaag attactgatg cgtttaagta cagttggtgc agtcttcctt ggtttagtgg
    10681 cattgcttcc aatcattgct caaatggttt gggatcttcc acaatcaatc ggattaggcg
    10741 gtacaagctt actgatcgtt atcggtgtag ctttagaaac tgcgaaacag ttagaaggct
    10801 taatgctgaa acgtaaatat gttggattca ttgagtaaga aagcgtgttg aggtttcccc
    10861 tcaacacacg ttctcacaca tttattagga gggaaaacaa tgaacctcat tttaatgggg
    10921 ctgccaggcg caggtaaagg aacacaagct gaaaaaatca ttgatgctta tggtattccg
    10981 cacatctcaa caggagatat gttccgcgca gcgatgcaaa acgaaacagc tcttggcctg
    11041 gaagcaaagt cttatatgga taaaggtgca ttagttcctg atgaagtaac aaatggaatc
    11101 gtgaaagagc gactagctga accagataca gagaaaggct tcttattaga tggctttcca
    11161 cgtacgctag atcaagcaaa agctctggac gcaatgctaa aagatttgaa taaaaaaatt
    11221 gatgctgtca tcgatatcca tgttggtgaa gaaatcttgg tcgaacgtct agccggccga
    11281 tttatctgca gcaattgtgg agcaacttac cataaaattt tcaaccctac aaaagttgaa
    11341 gacacatgtg atcgttgcgg tgggcatgaa ttctatcaaa gagaagatga taaacctgag
    11401 acggttaaaa atcgtctggc tatcaacatc aaaaatagtg aaccaatctt ggcttattat
    11461 aaagaacaag gtttgctaaa cactattgat ggtgctcgtg aaatcgatgc tgtatttgca
    11521 gatgtacaaa aaatcattga aaaatagcac gattgcccgt agccgacttg cattatgcag
    11581 ttactcttgt caagttcaat aaattatgat agaatatgtc agtccgactt ctagagaatc
    11641 ctctagatga tccattttga gaattttgag gtgggaactg cagtttccgc cattttatcg
    11701 tgtgaaaatg cgaaacaagt acaaggaggt actgtgcgtg gcaaaagaag atatgattga
    11761 agtcgaaggt acagtcgtcg aaactttgcc gaatgcaatg tttaaagttg aactagaaaa
    11821 cggacaccaa gttcttgcta ctgtttcagg taaaattcgt atgcattaca tccgtatctt
    11881 acctggtgac aaagtaacgg ttgaattgtc tccatacgat cttaaccgtg gccgtattac
    11941 ttatcgcttt aaataattgt actccgtaaa actacaggag gtattatcat gaaagtaaga
    12001 ccatcagtaa aaccaatgtg cgaacattgt aaagtaattc gtcgtaaagg acgcgttatg
    12061 gtgatttgcc cagcaaatcc aaaacataaa caacgtcaag gataattgga ggtgctataa
    12121 atatggctcg tattgcagga gtagatattc ctcgtgataa acgtgtagta gtttcactta
    12181 cttatatcta tggtattggt aacactactg ctaaaaagat cttagctgat gctggtgtat
    12241 ctgaagatgt tcgcgttcgt gaattaacga atgaacaaac agatgctatc cgtgcagaag
    12301 tagataaatt aaaagttgaa ggggatcttc gtcgtgaagt gaacttgaac atcaaacgtt
    12361 tgatggaaat cggttcttac cgtggaatcc gtcatcgccg tggattgcct gttcgtggac
    12421 aaaacacgaa aaacaatgca cgtactcgta aaggcccagc tcgcgctatc gctggcaaga
    12481 aaaaataatt cttaagtgaa ggaggttaaa acttcatggc agcaaaaaaa gtgaatcgta
    12541 aacgccgtgt gaagaagaat atagaagcag gtattgcaca tatccattct acattcaata
    12601 acactatcat catgatcact gatactcacg gaaatgcatt agcgtggtca tcagctggtt
    12661 cattaggttt caaaggaagc aaaaaatcaa ctccttttgc cgctcaaatg gcagcagaaa
    12721 ctgctacaaa agcagcaatg gaacacggat taaagactgt tgaagtaaca gttaaaggac
    12781 ctggttctgg acgtgaagca gcaattcgtt cattacaagc tacaggttta gaagtgactg
    12841 caattcgtga cgtgactcca gttcctcata atggtgcccg ccctccaaaa cgccgtcgtg
    12901 tttaatgagt gccgctcatt ttgagtttta tcagtaacgt cattgaacaa gacacttttc
    12961 gttttgaaag gggtaaagat aagaatgatt gagttcgaaa aaccaagaat cgcaaaaatt
    13021 gatgaagaaa aagattatgg caagttcatc gttgaacctc ttgaaagagg gtatgggact
    13081 actttaggga attccctacg tcgtatttta ttatcttctt tgccaggtgc tgccatcact
    13141 aatattcaaa tcgatggtgt actgcatgaa ttctctacca tcaaaggtgt tagagaagac
    13201 gtcactcaaa tcatcttgaa cattaaaggc ttagcgctga aaatgtatgg ccaagaagaa
    13261 aaaacccttg aaatcgatat taccggacct gctacagtaa ctgctggcga tattatcgtt
    13321 gatagtgatg tagaaattct taacaaagat atgtacatct gtagtgtctc tgaaggagct
    13381 acgttccatg ctcgcttaac agtgaaacct ggacgtggtt atgttcaagc agatgaaaat
    13441 aaaaaagaag atatgccaat tggcgttctt ccagtcgatt caatctacac acctgttcgt
    13501 cgtgtgaact atcaagttga gaacacacgt gttggacacc gtgaagattt tgataaattg
    13561 acgatggaga tttggacaga tggttcaatt gaaccgttgg aagcaatgag tttgtctgcg
    13621 aaaatcatga ctgaacattt ggatattttt gtgaacttaa ctgatgaagc aaaaaatgct
    13681 gaaatcatgg tcgaaaaaga agaaacacaa aaagaaaaaa tgttggaaat gacgatcgaa
    13741 gagttagact tgtctgttcg ctcttacaat tgtttgaaac gtgcaggaat caatactgta
    13801 caagaactta caaataaatc tgagcctgaa atgatcaaag tccgtaactt aggacgtaaa
    13861 tcattagaag aagttaaagc aaaattgcac gacttaggtt taggtttacg taaagaagat
    13921 taaaaccttt tacgaaggag ggaaaccaac gtgagttatc gtaaactagg acgcacatct
    13981 agccaacgta aagcgatgtt gcgtgattta acaactgatt tattaatcaa cgaacgtatt
    14041 gtgactactg aagctcgtgc gaaagaagtt cgctcaactg cagaaaaaat gatcacttta
    14101 ggtaaacgcg gagatttgca tgcacgtcgt caagctgcgg ctttcgtacg taatgaagtt
    14161 gccagcgtgc gtgaagaaaa tgaggacatc gtcattgaat cagctttaca aaaactgttc
    14221 aacgatattg cacctcgtta cgcagaacgt caaggcggat acacacgcat tttgaagaca
    14281 gaaccaagac gcggtgatgg tgcaccaatg gttgtcttag aacttgtcta aaatcacact
    14341 aataacatca ctttatagat gattctttta gagtgttatg atgttggaag atatttttcc
    14401 gagtctagct cagcgctgct cctcaagcga atatcgcttg gggagtaagc actcatcata
    14461 aagtgttttt ttataccttc aattacctgt catgacagat atgtttgaag gcataaaaaa
    14521 acattttaag cgagaaataa ttgaaaattt tgtctttttt tgatagtgtt agtaggaact
    14581 ttaggatgaa gaaggagtaa tcatggagcc aattattgaa ttagaaaaga taaattataa
    14641 ataccagcca gatgatcttc gccccgcatt gaaagatgtc tcctttacaa ttgataaagg
    14701 tgagtggatc gctattattg gacacaatgg ttcaggaaaa tcgacacttg ccaaaacgat
    14761 caatggattg cttctgccag aatcagggat cgtgaaagtg ggaaatcaaa tattggatga
    14821 agaaaatatt tggacgatcc gacagatggt tggaatgatt tttcaaaatc cagacaatca
    14881 gtttgttggt tcgacagtag aagatgacgt tgcttttggt cttgaaaatc aagggattcc
    14941 cagagaagaa atgctagtgc gtgtgaaaga tgcacttgag aaagttagaa tggctgagtt
    15001 tgcttcacgc gagcctgctc gtttatcagg agggcaaaaa caacgtgttg ccattgccgg
    15061 cgtggttgcc ttacgacctg atatcattat tttagatgaa gcaaccagta tgctagatcc
    15121 agaaggtcgt gaagaagtga tctctacgat caagaagatc aaagaagaaa gtcaattgac
    15181 cgttatatct atcactcatg atattgatga agcagctaat gccaatcgga tactagtgat
    15241 gagacaaggc gaacttgtcc gtgaaggtac accaaaagaa attttttctg ctggtcctga
    15301 actgatcgac ttaggtctag atctaccatt tcctgaaaaa ctgaagagcg cactgaaaga
    15361 acgtggggtt gatgtgccca gtgaatatat gacagaagaa aggatggtgg attggttatg
    15421 gacatccgtt ttgaacaagt agacttcact tatcagccga atacaccatt tgaacaaaga
    15481 gccttatttg acatcaatat gacgatcaaa gaaaacagtt atactgcatt agtagggcat
    15541 accggaagcg ggaaatcaac cttacttcag catttgaatg cgcttgttaa gccaacaagc
    15601 ggtacagtcc atattggaga acgagatatt cagccggata cggataataa gaatttaaag
    15661 cccatccgaa aaaaagttgg catcgtcttt cagtttccag aagcacagct attcgaagaa
    15721 acggtagcaa aagacattgc tttcggtcct aaaaactttg gtgtcagcga agaagaagca
    15781 ctagtcctag caaaagaaac attagaacaa gttgggctgg atgaaagcta tttggaacgt
    15841 tcgccatttg aactttcagg agggcaaatg cgtagagtag caatcgctgg tgtgcttgcc
    15901 atgaggccgg aagtacttgt attggatgaa ccaacggcag gacttgatcc ccagggcaga
    15961 aaagaaatga tggagatgtt ttggcgtctt cataaggaac agcagatcac tattgtatta
    16021 gttacgcatt taatggatga tgtagccaat tttgcagatt atgtctatgt gttggaaaag
    16081 ggacgaatcg ttaatagcgg ggagccgcaa gaggtctttc aaaacattga atggctaaaa
    16141 gaaaaacaac taggcgtacc tactgccacc gagtttgcag aagaattaat ggctaaaggg
    16201 atgaactttg ctaccttgcc tttgacagct gaagaattag cagacgctat cgtccattat
    16261 gctggaggaa atgcccatga tgaataaatt gatatttggt cgttatatgc ctggagattc
    16321 cttgatccat cgattggatc cccgagctaa attattagcc agtttttatt ttatcggaat
    16381 cattttttta gcaaataatt gggaaacgta ccttttgttg gcatttttca cattactttc
    16441 tgtcttcttg tcaaaagtag atttgaaatt ttttatccga ggcattcgcc cattgatctg
    16501 gctaatcctc ttcactgtag ctttgcaggt cttgtttaca agtggcggag aaacttattg
    16561 gagttggggt gttttccata tcactgaatt tggtgtgatg aacggcctct ttattttctg
    16621 tcgattcgtg ttgatcattt ttatgtcgac tctactcacg cttactacac cgcctttgga
    16681 attatcggat gcgattgagt acttgcttcg tccattgaaa gccgtacggt tcccagtcca
    16741 cgaaatttca ttgatgctgt cgattgctct tcgttttgtg cctacattga tggacgaaac
    16801 agaaaagatc atgaacgctc agcgcgctcg cggtgttgat tttggtgaag gaaacctagt
    16861 tcagaaaatg aaagcagtca ttcctttgct gattccttta tttgtcagca gcttcaaccg
    16921 tgcggaagat cttgcgactg ctatggaagc aagaggctac aaaggtgggg aaggaagaac
    16981 gaaatatcgt gtattgcatt ggcataatag agacacgatg gttatcattg cttttgtatt
    17041 attaactgtg ctactgatct ttttaagagg ataaacgttc agtgggccgc tatctattct
    17101 tttcttgctg atctgtttgc aagaaaggat gcaagcagtg tatgacaaga aatattaggg
    17161 tctgtggcag actaaggtca caggcctctt tcttttatct gaaaagaaag aacgaaaaaa
    17221 ttttatagaa aaatcattat tccagataac cttttaggaa caaaggagag aaaaacatgg
    17281 tacgttacaa agcaatcatt gcgtacgacg gaacaaattt taatggattc caaaaacagc
    17341 caaatgggcg tactgttcaa gaagaagtag aaaaaacatt acaaaaaatg gcaaatggaa
    17401 aagaaatcac ggtctttggt tcgggaagga cagatgccgg agtacatgcg atcgggcaag
    17461 taatccattt tgattatcca gaggagcgtc cgttagaacg aatgcgtttc gcgctagata
    17521 cccagtcacc agaggatatt gctgtaagac aagtagaaat tgtttcagaa gacttccacg
    17581 caagatattt ggtcaaagaa aagacgtatc aatttcgagt ggatataggg aaaccacgca
    17641 gtccttttcg acgccattat gcaagttatt ttccttaccc acttgatttg gaaaaaattc
    17701 agcgggcgct tcctgattta ttagggactc acgatttcac ctctttttgt gcatcaggaa
    17761 gctcgataga ggataaaata cgtacgatct acgaggctaa gatggaggta aatgaaacaa
    17821 aagacgaatt gttgtttaca tttaggggaa atggcttctt gtataagatg atacggatct
    17881 tagtaggaac gcttttgaaa attggcaatg gccgattgcc tgaagatagt atcccagata
    17941 ttattgcgaa aaaagatcgt aatgcagcag gacctacagc acaccctgaa ggactttatt
    18001 tgtatgaagt gaaatatgaa taagaggtta tgatagaagt ggtcagcttc aagaaataag
    18061 aaggtattta cgaaaattac ttctcaaaat tttgcaaaat ttcggcttat tttcgaagaa
    18121 gctacttctg gaacaccgtt taagaaagag gtcgtgagcc tgtcatttaa ctctgagtca
    18181 caaagagcaa tttcaaaaaa cagcttctca tctttgatga atatcgtgac ttgtggacga
    18241 ggcgttgata agtgaatcat ctttttgtta cctatttttt ctgtaaccaa aaaacgatgg
    18301 aacgttgttt tgattgccct tggtgcggac agtctgaaca ggttccatcg tttctttatt
    18361 ttctacgtct tcttctgtgt gtgtttcgtg gttgttcttc tattgctgga tcaatcgtgc
    18421 cataaaaggg gcgattgcgg tcaaggtacc agacataaag aataaacaat gcaatacata
    18481 ggacaaaaag gattgcacct atcgtaaatc cttcaggcaa gtaactgaaa gtgacagtat
    18541 gtttacctgc tggaactttt atcatcaaga atgcgtcttt gaaattgctg accttgactg
    18601 gtttcccatc aattttagct ttccatcctt tgtcataagg aatagttgtc accaacatgg
    18661 aatcttcagc ggtatttaca gtcccagaag cttttctgcc agtggcttcc atttcgacac
    18721 ctttttcttt gatctgattc attgcggctt gataggctac aacatctaga ccaacgactt
    18781 taggctccat gaaacttatt tctttcgtcc cataaaaact tgcagtaaat gtcactgttg
    18841 tctcttgagg ataatagcca agatcatagt attgaccaga aatgttgatt tgcgtttttc
    18901 gcgatgtacc attgacagtg atcgtcgcac tagaactttc taattgcgca aaattggttg
    18961 ggaacaaact taagtaagct tgtgtatttg caggcacatg gacttcccaa gtgagctctt
    19021 ttggctggtt atctgctgtt tctgagtaag tgacgccagt cgttgtcttt gtgatcgtcg
    19081 tattcaactg attcttcaaa gtaggtgtat agaatgtaaa atactgttca tttagaccag
    19141 acaatgtatt gatcaagttt gtctgactgg tcaaattatc tgtcactggc tgctcaacat
    19201 cattaacgga caacggtgca gtaaatgcta gtggcaatgc attgacattc tgatacagtg
    19261 tatagtcgcc gctttgacca gcttggcgga agccatattt actgaacgtg ctttcgtttt
    19321 tagaaatgtt atatttgatt cctgtaaacc catccatcag caaagtatta ttgttataac
    19381 ggatattcaa atttgtccct cttgaacgga aacctaattt atccaggtaa gaggaagaat
    19441 tacgatttct tatagaagaa aacaaactga taccactgta accatagttg atcgaatcgt
    19501 ttgcagatac tggatccaga ttttccaagc ggtaaaacga gtcggaatct ttttttgttt
    19561 catttacaag agctttcaag tcaggataag gatcagtata taaactgcgg gaagcataat
    19621 tccaatcatc aagaatgcca tggatcatac tgttcgtatt gaccagtgct tcagcagaca
    19681 tgatcaataa taacaatata ctcagatgat tgaatgaaag ttttttatgc tgataaaatc
    19741 ccacagcaaa agcataaatc cctaagaaaa ccactgtcaa tacaaaggaa gtcatcgtca
    19801 cataggtgta gcttccttca ggtttgatgc cccatgccac cgcaaagaga gcagctacga
    19861 ccaaaatgat tcctaacaac agcccaccat cgtcttgttc tatcttttcc catccttttc
    19921 ctgcaagcag gagcactaag aaagagaaca gataggcata acggaataaa aacatattcg
    19981 gtgcatgcat accatgccag aacaaattca atggaacaag ataaaaactt gcgataagta
    20041 tggcgaaaag actgccgtac aaaaatttat ttttcaacgg tactttccga ctggcaaaat
    20101 aaaacagaca gaggactaat ggtagcagac ctgcgtaaac gaacggaatc gatccgtatt
    20161 ttgtcgtgtc ataaacacca atcatatttt tcatgattag atccagataa gaagtcgctt
    20221 ctgttttcaa cgtagtgatc tgactcagtg cctcgccatt tgaccgaaga tccagcactg
    20281 ctggcaatac aatgatcatt gaagcgccgc ctgccagtaa agaagtgata ccataaggaa
    20341 caatcgtttt tttatattct gaccagtttg tcaatagacg ggcgataaaa tataagaaag
    20401 aaaatacgcc aatcataaaa cccatataaa aactggataa aaacaagagt aaataactga
    20461 taaataaaag tttcggccgt ttaagatcca taacacgatg aatccccaaa atcactaatg
    20521 gaagataaac aaatgcatcc agccacatga tgatctccga atgtgcagtg ataaatgaga
    20581 tcaatgcgta actgacgcta agtgcaacat gcccccagcg aggaatcttg aacgtacgtg
    20641 caagaaacca aaaagccaat ccggcacagc cgacc
//
LOCUS       DACDMM010000037        20658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDMM010000037 DACDMM010000000
VERSION     DACDMM010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.037"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            179..2107
                     /locus_tag="IWD16_001042"
     CDS             179..2107
                     /locus_tag="IWD16_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9088122.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            2245..2700
                     /locus_tag="IWD16_001043"
     CDS             2245..2700
                     /locus_tag="IWD16_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP9088123.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            3170..3439
                     /locus_tag="IWD16_001044"
     CDS             3170..3439
                     /locus_tag="IWD16_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088124.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            3839..4093
                     /locus_tag="IWD16_001045"
     CDS             3839..4093
                     /locus_tag="IWD16_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP9088125.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(4182..5228)
                     /gene="holA"
                     /locus_tag="IWD16_001046"
     CDS             complement(4182..5228)
                     /gene="holA"
                     /locus_tag="IWD16_001046"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP9088126.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(5266..7539)
                     /locus_tag="IWD16_001047"
     CDS             complement(5266..7539)
                     /locus_tag="IWD16_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP9088127.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(7532..8071)
                     /locus_tag="IWD16_001048"
     CDS             complement(7532..8071)
                     /locus_tag="IWD16_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP9088128.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(8207..8425)
                     /locus_tag="IWD16_001049"
     CDS             complement(8207..8425)
                     /locus_tag="IWD16_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP9088129.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(8422..8667)
                     /locus_tag="IWD16_001050"
     CDS             complement(8422..8667)
                     /locus_tag="IWD16_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9088130.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(8783..9511)
                     /locus_tag="IWD16_001051"
     CDS             complement(8783..9511)
                     /locus_tag="IWD16_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP9088131.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            complement(9670..10713)
                     /locus_tag="IWD16_001052"
     CDS             complement(9670..10713)
                     /locus_tag="IWD16_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9088132.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            complement(10703..11194)
                     /gene="coaD"
                     /locus_tag="IWD16_001053"
     CDS             complement(10703..11194)
                     /gene="coaD"
                     /locus_tag="IWD16_001053"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP9088133.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            complement(11191..11751)
                     /gene="rsmD"
                     /locus_tag="IWD16_001054"
     CDS             complement(11191..11751)
                     /gene="rsmD"
                     /locus_tag="IWD16_001054"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP9088134.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            complement(11870..12208)
                     /locus_tag="IWD16_001055"
     CDS             complement(11870..12208)
                     /locus_tag="IWD16_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP9088135.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            complement(12192..12629)
                     /locus_tag="IWD16_001056"
     CDS             complement(12192..12629)
                     /locus_tag="IWD16_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9088136.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            complement(12619..13743)
                     /locus_tag="IWD16_001057"
     CDS             complement(12619..13743)
                     /locus_tag="IWD16_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088137.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            complement(13764..17192)
                     /locus_tag="IWD16_001058"
     CDS             complement(13764..17192)
                     /locus_tag="IWD16_001058"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP9088138.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            complement(17218..18381)
                     /locus_tag="IWD16_001059"
     CDS             complement(17218..18381)
                     /locus_tag="IWD16_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9088139.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            complement(18384..18686)
                     /locus_tag="IWD16_001060"
     CDS             complement(18384..18686)
                     /locus_tag="IWD16_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP9088140.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            complement(18800..20572)
                     /gene="recQ"
                     /locus_tag="IWD16_001061"
     CDS             complement(18800..20572)
                     /gene="recQ"
                     /locus_tag="IWD16_001061"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP9088141.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
BASE COUNT         5977 a         4288 c         3558 g         6835 t
ORIGIN      
        1 tcttatttga gaaatgttct ggttttttat tttctttaat aataatcatt ataaataata
       61 aaaacaacat caagcttaat caccaaatga taaccattat cgttctcatt tagctgttat
      121 tattatcaat tacattttta ttatgctatc cttgttttga caaggaggtc taataacgat
      181 gactaatcag aaaaaagctt ttgcagcaac aatcggctgc cttttattta tgatcttcgg
      241 ttttatcttc gaccgattct ctcttaccat caccccactt ttttatatca gtgccattat
      301 catcggtgga tataaacaaa catcggaagg cttgaaagaa ttgtggaatg atcggacact
      361 gaatgtggat ttattgatgg ctttagctgc tattggtgcc tgtttgatcg gtaattactt
      421 tgaaggagca atgttgacat tcatcttttg tttaagcggt gcattagagg aatacacaac
      481 taataaaagt caaaaagaaa ttactgcttt gatgaatctt caaccacaaa aagcccaatt
      541 attaaaagaa aatggacaaa tgcaagaagt agatgtttct caactaaagg tggataactt
      601 ggtatttgtc gctaaaggag cagctgttcc gatagatggc tttttagaaa gcacacaggc
      661 aacgatcgat gagtctgcat taagcggtga atctgtgcct gtagaaaaat atttaggcga
      721 tgagatgtat gcaggaacgc tgaatcaagg aaatcccatc acgattcgag taacaaaaac
      781 gagttccgaa actgtctttg ctaagattat ccaattagta gaagaagcgc agaatacgcc
      841 aactcaaaca gcttcattta tcgaacgaat cgaaaataat tatgtaaaat tgattttgct
      901 tgctgttcct ctaatgatcc ttctccccca ctttttccta ggttggagtt gggatgaaag
      961 cttttatcga ggaatggtat tattagttgt ggcttctcct tgtgcgcttg tcgcatctgc
     1021 tacaccagct tctctagctg ctctttcaaa ttctgcaaaa aatgggattt taatcaaagg
     1081 cggtatccat ttagaaaagc tttctgaatt aaaagcgatt gcctttgata aaacaggaac
     1141 gctcactaaa ggaaaaccag ttgtaactga cagctttttc tttgatgaaa aggatttggc
     1201 acaacaactt cttgttgcta tggaacaaaa aacgacgcat ccacttgctc atgcgatttt
     1261 gcaatatttc gattgcacaa tacctgaaga aatccaatac ttaccaatag aagaaattac
     1321 tggttttgga ttacaaacta cctatttagg tgcgcaatgg aaagttggca agcacgcata
     1381 tgatcctgag acgatgataa tcagcaaaga gattgcggaa atgatcgaac gtttggaaaa
     1441 tcaaggcaaa accgtgattt atctttctaa agatcagcag ttaattgctg ttttaggtct
     1501 gctagatatt ccaaaagcaa atacccaaca agtaatttcc tactttaaat cccaaaacat
     1561 ccatacaagt atgataaccg gcgaccattc tggcacagca aaggccattg ctgaacaagt
     1621 agggattgaa cattactatg caagctgtac tccggaagaa aaaacacaac tagtcaaaaa
     1681 agagaatgaa ctttaccaag taaatgcgat ggttggtgat ggagtcaatg atgcgcctgc
     1741 tttagcagcc gcttctttag gtgtcgctat ggggcaaggg acagatgccg ctatggatat
     1801 cgcagacatc gtcttaatga aaagcgactt aaataaattt gtctacagtc atcggctatc
     1861 tttaaaaatg cggcggatca tcaaacaaaa tattattttt tcgattcttg ttattctctt
     1921 gttgattgct tctaacttcc tgcaattttt aaatctacct cttggagttg taggacacga
     1981 aggaagcaca atattggtta ttctgaatgg cttgcgttta ctaagacctt tacctgatct
     2041 ttctgattct tcaaaaaaat gtaagtcttg tccactatac aaagcgcggc tgtcttctgt
     2101 caaataagca aagtgttttt ttatgatttt gtttatttta tgaaaaaaac agcttatgaa
     2161 taggtcttga aggatgaatt cctacttttg taactttcta aaaacatgtt atgattgttt
     2221 cgaagaaagt gaggaatata tttcatggaa agttggcttt ttttaggaat tattttactt
     2281 gtagcaattt tagctaaaaa tcaaagtctg atgatagcgg cagcttttgt tttgattatg
     2341 aagctcatac cagcctcccg ttcctttttc ccttggcttc aaggcaaagg aatcaatatt
     2401 ggggtgacga tcatcacagc ggctattcta atccctatcg caacaggaga aatcggtcta
     2461 cgtgatctat tagaagcctt taaatcccct attggctggg tagctgtttt ttgcggtggg
     2521 ctagtggctg tcttatcttc caagggtgta ggattgattg cttctgatcc acaaatcact
     2581 gttgcacttg ttttcggcac gattttaggt gttgtcttct taagaggaat cgccgccgga
     2641 ccggtcattg cttctggtat cacttactgt atcttacaag tatttgcttt aggaaaatag
     2701 aacaaatttt gtctatttta ggagattctt aacaccccca atttactgat ccgtgatata
     2761 ctgacaacga ggtgatacaa tgttcgaaaa attaaagtat ttcataggaa caatcggctg
     2821 gtttcttcta gcggtacttt tgattctggg cttagcttat ctattatttc ctgatttgat
     2881 acaaaatgtg tttgatactg ctttttgatt gacctattaa aacgatatag gggatgctcc
     2941 tgcgatgcag gggctttttt taatcaatgt tttgtaataa tttaggccgt gccaatgaga
     3001 cttcctatag aattcgatgc tcttttttct ttttggctct tctgctcttt tttgttatac
     3061 tagattacag tggacaaaag aaagaatgat cgcatccact tcaacgaata tcaaaatgga
     3121 agaattagtg cctgttttca ggttactttg aaaagaaagg aagacgcttg tggaaacttt
     3181 tgaaggtctt ttaattgata cgtacgtttt acaagaacag ccaatgatgc tgactgggat
     3241 caacttgcag cttgaggacc gtgtgatcaa tggcctgctt aaagatcgct atttggcaaa
     3301 atatatctta cgtttgcccc taaataaata tgtgctggaa gtaagtggca aatggaaccg
     3361 tcggaaccag ttggtcataa aaaaaatgtt gataaaaaat atcgatgaat acattaaaat
     3421 aatcggtaca ccagaatagc ctttactgat caaaggatac tggttataaa ataactgcag
     3481 tttcttaata gaaaaacaaa gaacaacagg gaatcgtatc ccttttcaaa aaaaactttg
     3541 caaacggttc tctttgttat ataataactt agtaccaaac attcccattc aaactttgtt
     3601 taaatagata aataaaaatc tagtttggtt tgacgctatt gcctcccaca atagcgtctt
     3661 tttgattttt ttcagcttgt actatccgtt tttactgaaa aaaaatccta ataaaagact
     3721 gttaaggaaa aaaacttgtc atgttcatat tgcaaagcac gctgccttta ggtataataa
     3781 tgaatgttga attatgcggt aacacgcgta agaacttaag ttatcggagg tgaaactaat
     3841 gccaaacatt gaatctgcaa tcaaacgtgt tcgtacaagc gaaaacgcta atgtgaaaaa
     3901 ttcatctcaa acaagcgcta tgcgtactgc aatcaaaaaa tttgaagatg ctgtagcttc
     3961 aggtgctgac aacgtagatg cgttatataa agaagctgtt aaagctattg atatggctga
     4021 atcaaaaggt ctaatccaca aaaacaaagc taaccgcgac aaatctcgtt taagcaaaaa
     4081 aatcgctaaa taaaagaatc ggcttcggcc gattttttta tttgtcaaaa aaaggacaaa
     4141 aacaaacgac cttcccgcat gctttgccct cgtctttctt cttatcgatt tcttaacaac
     4201 tgccctgtgc gcaaaacgaa tagttgaaaa cttaattctt tttcgatttg tcccgttttg
     4261 atttgataat ccatctcaac taattgatca aacaactgta ccagcaaatg ttcatcgaat
     4321 ttttttactt cctgcatggc aagttttaca cgatatggat ggattttcaa tgtttcagca
     4381 atatttgcct gctgataacc aatctttaca agaaattttg tctgtaaaaa taaacgtaat
     4441 tgagaaagca aaatcgcatt aattttgatt gtttcttccc cttgcataac taaatcttcg
     4501 tataaacgca aagcttgttc cgtcttccct tttaaaatat attgggtcat gtcaaaaata
     4561 ttatgttcta acgttttagg aactaattct tctacttctc ttaccgtgat ttgctgttga
     4621 ttttgtccat aaaggaccaa tttctgtaac tctctcatca ttttggataa atctaagtcg
     4681 gtcaacctca agaataaatc aattgcactt cgttccattt tgattgagga attctctaag
     4741 gtattcatca gatattgacg tacttctttt tcattcaact gttggacatt gatgataatt
     4801 gctgtttttt tcaattgttt cgttactttt ttccgttcat ccaacttttc atatggcgca
     4861 aagaatacta atacagtcga ttctaatggt tctttcaaat aagccgtcaa taaatcagta
     4921 ttttgttcga ttccattgtt tactttttca cctgttaaaa aataaggatt ctctacgaat
     4981 actaaccgtt gatctccgaa aaacggcaac gtctctgctt cagctactac ctcatctaaa
     5041 cttgcatttt ccatgtcaaa acttaaaaaa tttaagtcat cttcgccctc taattggatt
     5101 tttttgacaa tttctgtccg aacttgatct tggagaaact tttctgttcc taaaatcaag
     5161 taacaagatg caagtttttc tttacgtatt ttttgtaact ctgcttgtat actcaaatta
     5221 ctcaccactt tcaaaaatta tcctaccatg agcagatgag aattactagt ctatcattgt
     5281 ttccactagc ccatgaggat gaaaaactgg atgccagcga taggtgatca ttccttgttg
     5341 atccgttcgc catgttttta tctgattttc ttctagtgtc tccaatactt cgctgtgcgg
     5401 gtgaccataa cgattattta cgccgcagga tataatccca tgttttgctt caatatggga
     5461 aagaaaatct gtgttacttg acgtacggct accgtgatga cctaatttga ctacatcggc
     5521 tttcagatta ggataaatca aaagcaattg tttttccatt gcttgattca aatctcctaa
     5581 aaataaaaag cgcctattgt tatattccat ccatagacca atcgaatctt catttcttcc
     5641 ctctcccttt tcaaaaggag tcaatacata aagatcataa taaccgctga tcctgtcccc
     5701 atgtttgatt tcaatgatct cagtaccatt tttttgagct tcagataaat gcctttgtaa
     5761 tggctcattt cgtccagcgc cccaaccgat ataaattttt ttgatagtaa aatgctgaga
     5821 taacgtgggt aattctccca catgatccgt atcgtcgtgt gtcaagatga gtttatcgat
     5881 tacacttatc ccctgacctt ttagatatgg aacgatattt ctttcagcag gaggacgtat
     5941 cataccttgc gcccactttt ctctttcaaa gtgtaactga cctccagtat cgatcaaaat
     6001 cgtttctttt cgaaaaggac tttgcaaaaa gatactgtct ccttgaccga catcgataaa
     6061 agcgatcgtt gaagaaaagc gtaaaaatgg caaagtcaaa aaaataaaag aaaaaagacc
     6121 aacataaaac aagtgtttca caggttgttc tttcaaccta tccatacaaa tcagtaatcc
     6181 gaaaatatac atcccagata gcacaagggg caaacgtcct ataatgagag acgaccctga
     6241 agaaaaatcg attattcgct caaataagat taatccctct tctatcagca aaagtaaagt
     6301 ttccggcagc cagcgattga agagtgtgag aaataacaca ccaggaagaa aaagaaactt
     6361 gaataccggt gcaatcagta atgtaaaaag actacctaaa ataggccact cataaaaatg
     6421 ccatacaatc aacggcatcg tgatcatagt aaacaggacg tgagaggccg tttttttcaa
     6481 ccaatctcca tcaaaagaaa tcaggataaa caaaaaactc atgaaaaaac taagctgtcc
     6541 acccgcatga aaaagaaatc gaggatcaaa tagtaaaaga agccaaaaga caactgcaaa
     6601 taagtccatg gtggaataat gcttattcag caatttgact agcaatcgca ataaaaacag
     6661 caaactagca cgcaaaacag aaatagaact tcctgcaata gaaacataac agatagttag
     6721 tagactcaac ggaataatac tgacgtgtag tggccatccg atacgtcgaa ataaataata
     6781 cagccaccct aaataaaatt ggatgtgtag acccgataga ctgaacagat gcagcaaacc
     6841 actctcccta aaaatttctt ctggttctct gaatcctgca tctttatagc cccataataa
     6901 agaattaata tatacacttg ttttttgcgg aaattcagat tggacaaagg agataaaaga
     6961 tcctcttttt ctctgaagcc agtttcgcca gtgattttgc atggtgatct gttcaatctt
     7021 ttgaatttgg aaccgtccag aaattctatg gttcctgaag taagcttctt ggtcaaaatt
     7081 ataaagattc cgcttttttg ctggagagat gaactcgcct tcagcagtca attctattcc
     7141 ctttcctcgg aagttaagcc atgttttcaa ttctgcttct gtgtttactt gataagaaag
     7201 ctggagcttt tgtttattct ctgataacct tccttccatt gtcaaaaaat taccatttgc
     7261 acgaatcgta tctgtaaata cagtaatttg cgcagatgtt gttttttcct ctaaaaatga
     7321 atactcttgt agcctataag aaatggatac cgttatgcta cacaaaatac acaagtacgc
     7381 gataaaacgt ccttctttta gcgaaaacca taaaaaaatt ccggcaagaa gcaaacgata
     7441 accccaattg tcagtgtaat acaacaaaca gataatcatc gacgtaattg ccggaaaaat
     7501 caaccgattt cgaacatgcc tttctaaaac gctagacatg ttttcctgtt aattcatttt
     7561 gaagttttga aaaatgctgt gagtctaaag caacttggtg tacagataca cctaattgtt
     7621 cgatcaacgc ttgggcataa ggatcatttc gatagtcatg gagataattg atccctttca
     7681 caccagcttg caacaaagcc tttgtacagg ccaaacatgg aaaatgtgtg acatagattt
     7741 ctgaaccttc cgtcgggata ccaagttttg cacactgtag gagtgcattc atctctgcat
     7801 ggatcgttcg caagcagtgt ccatccacga cgtaacaccc ttcatctatg cagtggacat
     7861 ctccactgac tgctccatta tagccgccag caatgatcct tttttctcga acgagtgtcg
     7921 ctcctacttc taaacgtgta cacgtactac gtaaagataa cagtactgcc tgagccataa
     7981 agtactggtc ccaaggaatg cgttcattat gtgacttatc agtaactttt tctttattcg
     8041 tatcgcatgc ttttgaagat ttttctaaca tgttttatat tcctccgttt tttcaagttg
     8101 attaaaaagt actcattgct taaacaaaat attctttaaa tataacgaat catcctgtct
     8161 gacaaaagat cctacccttc actgccgaaa tgaagtccgt catccattag cccattataa
     8221 aaattcttcg aatccatttg ttcaagaaat tccgtataaa aatcgatact tcacaatcat
     8281 ggcgttttgc tacttctgtg agaacagaga tatattcttt gatcaacttg ttattccgat
     8341 atttttgttt tgtctcatcg actgctggtg gcgtgataaa gaaagcatga tcagaactat
     8401 agtctttttt cagctgaact atcatccact gcagattttt tggtacgttt ctttagaaag
     8461 ttgtttatgc tctgctagat cattcgtccc aatgagaata aatactttat ctgctgtata
     8521 gctaagtaca tctttggcaa agcgttctcg tagagcttga ctgttattgc cgctgacagc
     8581 cgtattcata atatgaatcc cagatatttt attttggaga tagacattca gcattggttt
     8641 ttttaatgtt tctttgcgaa caatcaagct atttcctatc caaaaatact acctcctcgt
     8701 cctgccaagc tcttagaatt ggattattct tagtatccat tagcgtccat ttattgctcg
     8761 tgccagtcga ttttgaatca ttctatttaa ttgtaacgaa gttttttagt ttttcaactg
     8821 tcttttcacc gataccagaa atctcctgca ggtcttccac tttctggaag gatccgtttt
     8881 cttctctaaa tcgaatgatt tcttgcgctt tgactgatcc gatcccagga atctgctgaa
     8941 gttctgatgg agtggctgta ttgatattta ttttgggagg ctcggaatct gctgtaggct
     9001 gttttccttc cgaggatgcc aactgatcaa gatgttctgg cagctcttca cctacagcag
     9061 ggatatatag gacttgctga tcttctatct tcgcagaaaa gttgattcga tcgctatccg
     9121 cttcttctaa taatccacct gcacgcttta gtacatcata gacccgttct tctgaagaaa
     9181 aggaatagat accaggctcc ttcacacttc ctttgatatc ggtgtacatg acctttggac
     9241 gagcatcctc ttctacatcg tttccttcag ctgatggatc ttctccactg gaactaaacg
     9301 aagaaagtat ttcttttgca gcagtctctt ctgtttctct ataaaatagc aaatttgata
     9361 ataaaaaacc acttgcgata caaaagataa gaactatcat agacatccaa atgatcaatt
     9421 tgggtgaagc tagtattttc ttgctgattt tttctatcat tgttcttttt tcaaagaatg
     9481 accagtaaaa atcattcatt actcctttca tttaaaattg aagaaacagt gagctctggc
     9541 aaaatcgtcc cttcaacatc taattacgtc aattttttct tttgttttta tttttatcgt
     9601 gaagattttt gacgaaaaag aggggttgtg acagaactcg tgtcacaacc cctctttaat
     9661 tatttagttt catttcattt tctctagata gtcaagcgct tcttgtactg tttttactgg
     9721 tacgattttc attgaggaat tcaatttttt agcggcagct tttgcttctt cgtagttggt
     9781 ttttatttca ggatattctt tcttaacatc gtctgaaatc gtatcatctg gtgcaaagaa
     9841 aatctctacg ccagctttat ccgcacttgc cactttttta tcgattcctc cgattcggcc
     9901 aacttctcct tcactgttga tcgttccagt tcctgcaatt tgcttaccat gtcgcaagtt
     9961 tttaccagtg acttgttcat agatttctaa agtgaacatc aatcccgctg atggcccacc
    10021 gatagagcca gaattaatac gaacatctgt atctgattcg atctcggtat ggtcagtaag
    10081 cgtgatacct attcctgcct ttttatctgt tggtaactcg atcagatcac cagaagctgt
    10141 tttttcttca ccattatgca aataactgat ttcgattgtt tgtccaactt tttggctttt
    10201 tacataatcc ataaatgctt ctgtactttg aaagcttttc ccgtctactt ttgttactgt
    10261 atctccaact gaaagcttgc caaagaaatt cgatgttttc tctacactca tgacatatac
    10321 gcctttgaat tccattttat aaggcttgtc tgcaagtttt aacgcttgtt cgattgctgc
    10381 attttgggaa gaatccatat aatactgttg catttgatta tattcttcat ttgtcgcccc
    10441 accagtcaat tcttcttcac tgatcaattc ttcaaaaggg gtcattttag cttttaaagc
    10501 taagaaacct gtagcttgcc tgataccaac agaggtaaga gagaaagacc cagaaccttt
    10561 atcttcctta tcattgaccg taattagttc tttcagatcg atcgtggctc ccggtgattc
    10621 tacataatac ggcaagggaa ctactacaaa tccaatgatc aacattccaa atatgataat
    10681 cagtactgtt ttcaaccatt gtttagcttt catttgattc acgctttcca gctaaagctt
    10741 cgttgatcac tggcggtaag tagctgctca catctccgcc aaagtgcagt acttctttta
    10801 aaatactgga actcacgtga ctatattctg gttttgctaa taagaagaca gtttccaatt
    10861 catcatatag atgatgattc atctgagcaa tctctcgttc atattcaaag tctttgatac
    10921 ttcgtatgcc tctgatcaac gcatctgcac caatctcttt tgctgtttct accgtcaatt
    10981 gattttcttg atgcatgact tttacattag gaagatgaga aacagctttt gtgatcaatg
    11041 tcattctttc ttctggggga aacaaacttt tctttgaagt attgataaaa acaccaatta
    11101 ctacctcatc gaataattta gccgcacgtt ccaccgtatc taagtgacct tttgtaaaag
    11161 gatcaaaact tcctgggaat aatgcacgtt tcattgattt ccctcttttc tatagatagt
    11221 tacggccgtt atgccataaa tctgttttct tatttgatgg aatccagcca cagtttctgg
    11281 caattcgact gttttatccg tttcgcatac gataactgcg gttttagaaa aaagtcccag
    11341 ttcttccatt ttatggattt gatcgattat tttttgtttt gcatatggcg gatcaagaaa
    11401 caagtagtcg aattgttgtt tctccgctgc tagccgttcg attgctttgt ccgcatcaag
    11461 ttttattgtg gtaaatttct ctggttcttt cgtaatttcg atgttttctt tgatgatttt
    11521 tagcgcttgg taatttttgt ctacgcatac cgcatgactg cagcctctag atactgcttc
    11581 gatagctaaa ccaccgcttc ctgcaaacag atccaatact gtctctccat caaaatatgg
    11641 tccaatcata ttgaatatgg attcttttac tttatctgta gttggtctgg tgttcgcccc
    11701 tgctaaactt tttaaacggc gcccgccata atttcctgat attatccgca tctactcact
    11761 ccttagaaat ggtactggta ctagtatacc aaaaaaatac ttggacaatg atcaaaaatc
    11821 gatcgttatc caagttattt tttatttaaa aactgctcat aagagatttt taaagatgtt
    11881 cagctaatcg gatttctgta tttagctctt caatttcgaa accttcgtct tttaatactg
    11941 ttttgtttcc tattttttcg gcaaaattca tttcgatatc cggtcggtaa gaacgttcta
    12001 cgtttcgaac aaaatgtaat ttacggattc gttcttctgt ttcttccaat gtctcttcat
    12061 ttaagtaaag cacgacatat ttcattcttc ttgagacata atgcaacaag ccgaactttt
    12121 tcagattctt taattgtttt aagctgtaga cccatacgat cagcccgcgt cgttttgtta
    12181 tttcaaattc actattgtct aattgcatga caattacctc cgcatccaaa gtgttttttc
    12241 gtttcaaaaa acgggctacc tgcatctact ttgatttcat tagaaacttc ttcggcaagt
    12301 acttggacaa tttgatccaa cagactttgc agctgtgttt cactatatcg gaaagctgca
    12361 acatgctcat ccatatcaag ctgtcttttt gcttttcgaa cagctcgttt tttttcacga
    12421 taatcaggtg catactcacc atatgccgct acattttcaa atgcttcttt cttagacaag
    12481 aacgcttctt tcaactggca aacttttgca gattcttcca ttgactgttt atgttcaaga
    12541 taagaagcca tcgttttact cttttttatc atttcagcga cttggaggca ttgatcctcc
    12601 agttgaaaca gctcttcgtt aacgatcact gctatcacta ctttctccgt tttcttcttg
    12661 caataagact aacatatttt ctttagaaaa agcaatgcct acagaatccg ttcccgggtc
    12721 catgaaacgg tcatggttgt aaggatcgct gtaccaatat aaaaacgaaa aagtagcgtc
    12781 aatcgccgga tgaataaata gcccactagt attttctaat ttttgtttac tgattaactt
    12841 ctccaattca tcattcgtca gtgagaattt cactgtcgtt tgagcactgc ctagcgattt
    12901 ttgccaatca gccatacgtt cctctgacaa tatttcctca ggccttgtca aaaaatcatg
    12961 tagtagcagc tgactgtggt tttgaaaggc aagtgtttga ttgtatatgg gaagttcgtc
    13021 ttctcttcta agcatattta ataaagtcgt actttttcgt tctttaacgc gattgattga
    13081 ttcccaatca gccgatttat ctgcttgata atgtggaagg ccttcaccaa acacttgata
    13141 tggcaatagt ttcaaaagac tatctttatc taaatacaca actgaaaata ggccgtttgt
    13201 tgtttgatca aaaaataaaa tagcaaaagt gtcgttatca aaagcaatta gcggacgata
    13261 attcatatcc tcttccatta gttcgattcc aacttctgta tcatggtact caaaagtgaa
    13321 attagtatag atagtagtga ttttcgtaag atcttgcatc gtcatcccta aataaaacgg
    13381 cgcgatatca gcaggccctg tttgcagtac tttaacagtt gtgactctgc tgtcttggat
    13441 gttcgcttca aatgtgagat gactagcttt attttcataa tatcttgttt caaaaccaaa
    13501 tccgctagtt aatcgatcat aggcttggcc aaaatccttt tccagctgat cgatcggttt
    13561 tccgatataa gtagaaaaac cagttgcttt aagctcctgg tatttccacg ttgtcatttg
    13621 gtttgatatt tgttgatcag agttttcctt cacttcaagt tttggcggaa acaagactgg
    13681 ttccatgtag aatgccacaa taactaatag cagaatcagc caaaacaaac ccagtctttt
    13741 catgtttccc cacctttcgc ttattatttc tcagtcactt caatcaaaag gtcaccagac
    13801 gaaatagtgt ctcctgaaga cacatataca tgtgcgactt ctccgtcaaa acgtgcttcg
    13861 atggccgttt ccattttcat cgcttcagta atcatcaacg tatcaccttt tttaacgtta
    13921 tcgccttttt tcactaatac atccaaaaca gaaccagaca tcgttgctcc aatttgttct
    13981 cggtttgtcg gttcagcttt caatcgggtt tggaccgtac tgatgatcga attatctttg
    14041 atagtgattt cacgtctttg tccattcaaa ttgaagaaca gtacgcgatt cccttcgata
    14101 tcgggttctc cgatttcatc taaacgaatg ataagcgttt ttccgcgttc gatttgtaca
    14161 ttgattgttt cacccaaacg tataccttgg aaaaatgttg gtgtgtccag taatgtaaca
    14221 tcgccaaatt gattatatgc tttttgatag tccaaaaaga cttgcggata catcaaatag
    14281 cttaatactt cgtcttgttt tggttcatag ccaatttttt ctgccaattc tttttttact
    14341 tcgttgaagt cgactgattc agccaaaaga cctggacgtt cattcaatgc aggtctgcct
    14401 ttaagaataa tcttttgcaa ttttttagga aatccgccaa cgggttgccc tagttctcct
    14461 tggaagaaag tcacaacaga ttcagggaaa cttagtgttt cgcctttttc ataaatatct
    14521 tcttctgtaa gatcattttg gaccataaat agtgccatat ctccaactac tttagatgat
    14581 ggagttactt taacgatatc gccaaacatc aagttgacag tatgatacat ttgtttgatt
    14641 tcatcccagc gatgacctaa tccgacagct tttgcctgtt gctgtagatt cgaatactgt
    14701 cctcctggca tttcatgcat atatacttct gtttcaggag cattcaagcc gttttcaaac
    14761 ggtttgtaat acatgcgcac atcttcccaa taatggttta atttctgtgc attttcaatc
    14821 gtgatttctg gcagccgtgg accattgacc agtgcataat acaagctgct catgctaggc
    14881 tgactagtat tcccactcat tgcactcatt gccacgtcaa cgatgtctac tcctgctttt
    14941 gaagcagcag aataagtgat gattccattc ccgctggtat catgagtatg cagatggatt
    15001 ggcagatctg tcgtttcttt caattcacta atcaaacgat aagctgcctg cggtttaagc
    15061 agcccagcca tatctttgat tgcaatgata tgcgcaccca gttgttctag ttcctttgcc
    15121 atgtctttat aatactgaac attgtatttt gcccgacttg gatcgttgat atcgccagta
    15181 tagcaaatag ctgcctctgc gattttacca gtatcccgta cagcttggat acttttttcc
    15241 atttgtggag tccagttcaa actatcaaag attcgaaata catcgattcc ttgcgctgct
    15301 gcttctttga caaattccac caatacattg tcaggataat tagaataccc aacggcattt
    15361 gaacctctga aaagcatttg cagcaacgta ttaggcatta aagaacgaat ttttctcaac
    15421 cgttgccaag gatcttcatt caaaaagcgg taagccacat caaatgttgc tcccccccac
    15481 atctcacttg aaaagagttc aggaagacct tctccagtca aacgtgcaat ctgcttgaaa
    15541 tcctttgtcc gaacacgagt tgctagcaga ctttgatggg cgtctctaaa tgtcgtatcc
    15601 gtcaacaaca aattttcctg tcctttgatc cattcgacca aagcatcggc acccttcgca
    15661 tctaatacat tcttagccgt tacgtaatca gttcttgtga ttagatcttt aggcatacgt
    15721 ggttcttcat aaaatggttt ttcgccactt tcgatacctg gaaaaccatt cacagtaatt
    15781 tcaccgatat atttcatcgt tttgtttccg cggtctcgta gtcgaggaaa ttcaaataat
    15841 gttgctgtac tatcaatgaa ggttgttttt gcatttcctg attggaattc aggatgtgtg
    15901 atgacattga gcataaatgg aatgtttgtt ttcactccac ggattctaaa ttctttcaag
    15961 caacgttcca ttttctgaat ggctgtttca aaggtagcac catgcgtaca tacttttact
    16021 agcaatgaat caaaatacgg tgtcactaca tagcccgcgt aagcatttcc gacatccaga
    16081 cgtacgccaa aaccaccagg tgaacgatac gtatcgattt tccctgtgtc tggtaaaaag
    16141 ttattcaacg ggtcttctgt agtgattcgg cattgaatgg ctgaaccatt taatttaatt
    16201 ccttcttgct gaggcaatcc gatttcttta tgcagatcca aaccttgagc aatcaacagt
    16261 tgcgttgtta cgatatcaat atctgtaatc atttctgtga tcgtatgttc cacttggacg
    16321 cgagggttga cttcgataaa gtaaaaatca tctccttcaa caagaaattc tactgtacct
    16381 gcgttgacat acccgacata tttcatcaat tggacagctg cctggcagat tttttgacgt
    16441 tgctgttcat tcatagagac acaaggcgct acttctacta ctttttggtg acggcgctga
    16501 acagaacaat ctctttcaaa aagatggatg acgtttccat gcgtatcccc gagaatctgt
    16561 acttcgatat gcttaggatt agcgatgtat ttttcaacat acacttcatc actaccaaag
    16621 gctgcttttg cttcgctttt cgcacgttcg tagccctctc ttgcactctt ttcatcgtga
    16681 gctacacgca tgccgcgtcc gccgccgcct agagctgcct tgatcataat cggataaccg
    16741 tattgtttcg caaactccaa ggcatcgtct atagaagcga ttggtccatc tgtgcctgga
    16801 atcgaagcaa tccccgcttc gatagctgca gcttttgctt tgattttatc tccaaaaata
    16861 tctaaatgat gcagtttggg tccaacaaaa atgatgcctt cttcttcgca ccgttgcgca
    16921 aaattcaagt tttccgacaa aagaccgtaa cccggatgaa tagcgtcagc tccgcattct
    16981 ttcgcgatag aaatgatccc ttcaatatct agatacgcat caatcggttt ttttccttgt
    17041 cctactaaat atgcttcatc cgctttgaaa cggtgtacgg aatattcatc ttcttctgca
    17101 tagattccaa cagttttaat tcctaattct gtacaagcac gaaatacacg tacagcgatt
    17161 tcacccctgt tagcaactaa aacttttttc atcgaaccac tcctacatct ttttttatta
    17221 taaacctaag ctcttgcgtt tttcatccgc actgatgttg aggacaaagc cgacacaaat
    17281 cgaaagcatc agcaaactgg agcctccttg acttaaaaat gggaacgtga tccctgtcaa
    17341 aggaataacg ccagtaattc ctccaagatt gacaaatact tggatcaaaa acattgcgcc
    17401 gatcccaatg cacaaaagcg aattgaacgg atcttttgaa cggataccta caaggacaat
    17461 gcgcacaatc ataaagaata gcaacgctaa tatcaaaatc cccataatca ctcccagttc
    17521 ttccactacg attgcataaa taaaatcggt ttgtgcttct tgtaaaaatc cttttttctg
    17581 gatactgtta cctaatcccc tgccaaatag tcctccgttg aacatggcat agtagccgtt
    17641 gactagttga tgccctttat ttaattcgtc tgagaaagga ttttggtaaa tagcaaatcg
    17701 attatagata tactgtaaac gtccgggtaa gatctttccg tttgtgatat tgatcagcca
    17761 gatcgtaaaa gtacttagac aaaagcctcc tactcctaca atcaaagtat acacataatt
    17821 gactccgctg gctaaaagca gaaccaaaat aatcaaaaag ataactgccg cattaccaaa
    17881 atcgggaaga atagcgacta atgcagtaag tccgataacc attgcaagtg gacgtttcac
    17941 agtcccaaga aaatcttttt gtacactgtt ttgtctttta gataaagtca gagacaggta
    18001 ccagatagaa atgattttta ggtattcggc aggttggatc gagaatcctg caatttgcag
    18061 ccaacctttc gccccattga tctccttacc aaaaaacaca acaatcagca gcaaaatcgt
    18121 caaaactgcg atggctgcca tgatcaaacg ctggtttttc aaaacatctg ttttcatttt
    18181 ataaagtagt gcgatagcaa tcaagctaag cacccaaaaa atactctggt taatgactga
    18241 tgcggacgga ttttgtccgt tttctaataa tagatacgac gtagaactat ataccatgat
    18301 caatcccaat acacataaaa ttaaataggg aatcaagatg ctatagtcta atagatgcct
    18361 ttttttcact ttcttaggca agtctattcc tccttgccaa cttctttttt atcataaatt
    18421 tcttggtaaa cctcactata taactgattc aattctcgtt ctagctcact taacatcagc
    18481 tggccctcgt tctttccgat tactcctata cggacagcaa aaccgacttg ccgggaaaag
    18541 ccgtacatct gtgtatctac cacttcttcg aaggctttgc attgtgagat acagagactg
    18601 ttgttttgat ttcggattaa tcgtttgatc cgttctgctt catcatttaa tacttggatc
    18661 gcaagttttt tcgacaaaga atccatctgt gcttacctcc cattctaatt ccttaattat
    18721 aacatatttg attttcataa ggagcaaaaa aaacagattt aaccagttgt ttttattcat
    18781 ttttacgtat tcgttctttc tacttatcca gaacgttaga gtccgcattt tttatccgag
    18841 ataaaaaggc ttcgccatat ttatcaagct tgctttggcc aactcctttt atctgaagca
    18901 tctccaatct gcttgatgga cgtattcggc tcatttcttt caatgtggaa tctgaaaata
    18961 cgacatacgg gggaacgcct gcttctgatg ctagatccgt tcgcagttcc cgcagcactt
    19021 caaatagtgt atcggtttct tcatccgata gctgctggac tttcttgggt tcctttcgat
    19081 aaacagattc ttgtcctttc aatacttgta tccctctttc tgttactttc aaaacaggat
    19141 actcaccatt gatggtcaga agatagccat tcgatatcaa atattcaatt aactgcatcg
    19201 tttctttttg cgattggttt ttcatcagtc cataggtaga taaatgtcca aaacctaaag
    19261 cttgcagctt ttgttctttc gatcctgcta agactttcat cagcatttgt ttaccatagt
    19321 tctcacccat acgttttaag caagaaagaa ccatctgtgc ttctgtcgtt acttctacta
    19381 tttctctgtc atctaaacag ttaccacaac ggttgcacgg actgcttgtc tcgccaaaat
    19441 agtttaggat gtattgctga agacaggact caatatacac gtattctgtc atcgctttta
    19501 atttttcata ttctttttgc ttttgccctt cttctctttg ggactgctgg ataaaaaact
    19561 tttggacttg gatatcttga ggagagaaaa gcaggatggc ttcactggaa agaccatctc
    19621 ttcccgcccg accagcttcc tgatagtatg cttctagaga gccaggaatt tgataatgga
    19681 taacaaaacg gacattgctt ttatttatac ccataccaaa tgcatttgtc gctacaatca
    19741 gctgcaagcg atcgtataaa aaggcttctt gcatttctgt tcgctcattc tcatttagac
    19801 caccatgata tctacccacg gaaaaatcga attttttaag caaatgatag agacggtcta
    19861 cttcttttct cgttgctgca taaatgatac cagattgatt tttgttgatc tttaaatatt
    19921 cgattagata ttgttctttt ttttgatctt taattacttg aaatcgtaga ttttctcgtt
    19981 caaaaccagt ctgaatatgg ttgttttctg gaatttttaa aagtcttttt atgtcagcag
    20041 ctacttgcac agtcgctgta gctgtcaaag caacaattac aggacgattt ggcagttgat
    20101 ctagctggtt cgccatctgt aaataacttg ggcggaaatc atgcccccac tgagagatac
    20161 aatgtgcctc gtcaatagcg atcatacgga cgttccatcg cttcattgct tggataaaat
    20221 ccggcatgat aaaacgttca ggagcgatat aaagtaaacg gtactgctgt ctatcgattt
    20281 cttggaagcg tctagctgtt tcgtaaccat ctaacgagct attgataaaa gtggcgggta
    20341 tacctaattg attcgccgcg tccacctgat ccttcatcaa agagattaaa ggggacacaa
    20401 ccacagtaag cccatctaat agtaaagctg gaagctggta acagattgat ttcccacttc
    20461 cggtaggcat gattcctaat acattttcct gatcaatgac tttctggata atttcttttt
    20521 gaccaggtct aaactcatca taaccaaagt attgttttaa taagtcgttc atcttcccgc
    20581 tcctttctct tgccatttta cttcgatttt cttcgaagga caagttgatc tcctttcgtg
    20641 aaacattatc ttattatg
//
LOCUS       DACDMM010000038        20504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDMM010000038 DACDMM010000000
VERSION     DACDMM010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.038"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            13..222
                     /gene="yaaA"
                     /locus_tag="IWD16_001062"
     CDS             13..222
                     /gene="yaaA"
                     /locus_tag="IWD16_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP9088142.1"
                     /translation="MTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGEPENRRGRKLYP
                     GMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            209..1333
                     /gene="recF"
                     /locus_tag="IWD16_001063"
     CDS             209..1333
                     /gene="recF"
                     /locus_tag="IWD16_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP9088143.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1330..3276
                     /gene="gyrB"
                     /locus_tag="IWD16_001064"
     CDS             1330..3276
                     /gene="gyrB"
                     /locus_tag="IWD16_001064"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP9088144.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3296..5767
                     /gene="gyrA"
                     /locus_tag="IWD16_001065"
     CDS             3296..5767
                     /gene="gyrA"
                     /locus_tag="IWD16_001065"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP9088145.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6003..6302
                     /locus_tag="IWD16_001066"
     CDS             6003..6302
                     /locus_tag="IWD16_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP9088146.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6352..6864
                     /gene="ssb"
                     /locus_tag="IWD16_001067"
     CDS             6352..6864
                     /gene="ssb"
                     /locus_tag="IWD16_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9088147.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6890..7126
                     /gene="rpsR"
                     /locus_tag="IWD16_001068"
     CDS             6890..7126
                     /gene="rpsR"
                     /locus_tag="IWD16_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP9088148.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7269..9242
                     /locus_tag="IWD16_001069"
     CDS             7269..9242
                     /locus_tag="IWD16_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP9088149.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9248..9700
                     /gene="rplI"
                     /locus_tag="IWD16_001070"
     CDS             9248..9700
                     /gene="rplI"
                     /locus_tag="IWD16_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP9088150.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            9974..11341
                     /gene="dnaB"
                     /locus_tag="IWD16_001071"
     CDS             9974..11341
                     /gene="dnaB"
                     /locus_tag="IWD16_001071"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP9088151.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12121..13413
                     /locus_tag="IWD16_001072"
     CDS             12121..13413
                     /locus_tag="IWD16_001072"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP9088152.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            14149..15012
                     /locus_tag="IWD16_001073"
     CDS             14149..15012
                     /locus_tag="IWD16_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9088153.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(15141..16763)
                     /locus_tag="IWD16_001074"
     CDS             complement(15141..16763)
                     /locus_tag="IWD16_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9088154.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            17012..17533
                     /locus_tag="IWD16_001075"
     CDS             17012..17533
                     /locus_tag="IWD16_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088155.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(17719..19449)
                     /gene="spxB"
                     /locus_tag="IWD16_001076"
     CDS             complement(17719..19449)
                     /gene="spxB"
                     /locus_tag="IWD16_001076"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP9088156.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            19614..20432
                     /locus_tag="IWD16_001077"
     CDS             19614..20432
                     /locus_tag="IWD16_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP9088157.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
BASE COUNT         6547 a         3753 c         4366 g         5838 t
ORIGIN      
        1 aatacggaat tcatgacgtt gggccaaatg ttgaaagaag tcacagttat cggtagtggc
       61 ggacaagcaa aatggtattt ggcagaaaac actgtgttag tcgatggcga accagaaaat
      121 cgtcgtggac gcaaacttta tcccggcatg atggttgaag ttccagaagt aggaactttt
      181 tttatggtaa aaaaaggaaa cgacgataat gaggctgaat aagctgtatt taaaaaatta
      241 tcgaaattac gaagagctga acatcgaatt ttccaagaac ctcgtcatct ttttaggaga
      301 aaatgcgcaa ggaaaaacga atcttttgga aagtatctac gtattagcta tgacgcgaag
      361 tcatcggaca agtagtgagc aggaattgat tggctggact gacgatcagg cgatgattca
      421 aggagagatc acaaaaggtt catccactct tccattagaa atcttattat ctaaaaaagg
      481 ccgaaaaacc aagatcaacc atatcgaaca gaaaaagtta agtacttacg tgggacaaat
      541 gaatgtcatt ttatttgcac cagaagatct ttctttagta aaaggcagcc cgcagaccag
      601 gcggaagttt ttagatatgg aattaggcca gatcaatcca atctatcttt atgatcttgt
      661 ccaataccaa gcaattttga aacaaagaaa ccagtacttg aagcaattga atgaaaaaaa
      721 acaaactgat ttgctttatt tggatatttt gtccgaacaa ctagcagcgt ttggcagcaa
      781 agtattgaaa gcaagagcac agttcgtcaa gcgattggag ttttgggcta attctttgca
      841 tcagcaaatc acccatcaaa aagagcagct ggaaatcgag tatctcaccg cagtcgattc
      901 tttagaaaca catacgcaag agcaaatcca agaacaattt ctcgcacttt tgaaccaaaa
      961 taaaaagaaa gacctttttc ggggaacaac gacagttggt cctcatcgtg acgatctttc
     1021 cttttttatt aatcagaaaa atgtgcagac ttatggatcg caaggacagc aaagaacaac
     1081 tgcactaagt gtcaaattag cagaaatcga tttgattaaa gaagagacag gggaatatcc
     1141 aatccttttg ttagatgatg tgatgagcga gctggatgat aatcgtcaac tccatctgct
     1201 agaaacaatc gaaggaaaag ttcaaacgtt cttaacaaca acgacacttg accatgtaaa
     1261 aaataaaatg actgttgaac ctgagatttt ttatgttcaa caagggaaac taaaaggaga
     1321 agaaactgaa tgacagaaga aagaagttta gtagaacgcg ctaaagagta tgatgccagt
     1381 cagattcaag tacttgaagg acttgaagcc gttcgtaaac gacctggcat gtatatcggt
     1441 tcgactagtt ctgagggtct gcatcacctt gtttgggaaa ttgtcgataa ttcgatcgat
     1501 gaagtattag caggttttgc aacgaaaatc catgtgatta ttgaaaaaga caacagtatc
     1561 accgttattg acgacggtcg ggggatacct gtagatatcc aggcaaaaac tggacgtcct
     1621 gcagttgaaa cggtctttac tgttttacat gctggaggaa aattcggcgg tggtgggtac
     1681 aaagtttctg gtggacttca cggagtagga tcttctgttg ttaacgcgct ttctacacag
     1741 cttgatgtca aagtatacaa agaaggaaat gtgtactatc aagaatatcg tcgtggagct
     1801 gtagtggacg acttaaagat tatcgaacaa acggatcgcc atggaacaac ggttcacttt
     1861 acgccagacc ctgaaatttt tacagagaca actgagtttg atttttcaaa attggctacc
     1921 cggatccgtg aacttgcttt cttgaaccgc gggatgcgta tatcgattga agacaaacga
     1981 gaagaagaac cagtcatcaa tgaataccat tatgacggcg ggattaaaag ttatgtcgaa
     2041 tacttgaatg caaacaaaac cgtgattttt ccagaacctg tatatttgga aggtgaacag
     2101 caagatatcg cagttgaagt ctctatgcaa tatacagacg gctaccattc caatatcatg
     2161 agttttgcca acaatatcca tacgtatgaa ggcggaacac atgaatcagg attcaagact
     2221 gctttaactc gtgtgataaa tgattatgcc cgaaaacaaa agctgatgaa agaaaatgac
     2281 gacaacttga ccggtgaaga cgtgcgagaa ggattgacag ctgttatttc tatcaagcat
     2341 cctgaccctc aattcgaagg acaaacaaaa acaaaattgg gcaattccga agtacggaca
     2401 gtcactgatc ggttatttag cgaacatttc atgaaattct tgctggaaaa cccaagtgtg
     2461 ggccgtcaaa ttgttgaaaa aggattacta gcttccagag ctcgtctagc agcgaaacgt
     2521 gcgcgtgaag taacgagacg taaaggtgct ttagagatta gtaatctgcc aggaaaatta
     2581 gcggactgct ctagcaacga tcctgaaaaa tgcgaattgt tcatcgtcga aggagattcg
     2641 gccggcggat cagctaagca aggtcgtaat cgagagtttc aagccatctt gcctattcgt
     2701 gggaaaatct tgaatgttga aaaagcaagc atggacaaga tcttggcaaa tgaagagatc
     2761 cggtctttat ttactgctat gggtacaggg tttggagaag attttgatgt atcgaaagct
     2821 cgctaccaca aattagttat catgactgat gccgatgtcg acggcgcgca tatccgtaca
     2881 ttgctgctga ctctttttta tcgttacatg cgtcctattg ttgaagctgg atacgtctat
     2941 attgctcagc cacctttata cggggtaaaa caagggaaga atattacgta tgtacaacca
     3001 ggaaaaaatg cagaagaaga gttgaaacaa gtcgttgctt cacttcctgc ctcaccaaaa
     3061 ccaagcgttc agcgttataa aggacttgga gaaatggatg atcatcaact atgggaaaca
     3121 actatggacc caagcaatcg gatgatggcc cgcgtcagtg tggacgatgc tattgcagca
     3181 gatcagatat ttgaaatgtt gatgggggat cgagtagaac cccgtcgtgc attcatcgag
     3241 gaaaatgcac actatgtgaa gaacttagat atttaatcat gaaggaggaa aaagaatgag
     3301 tgaagaaatc agagaaaaca tccatgatgt caatctaacc agcgaaatga aagactcctt
     3361 catcgattat gcgatgagtg ttattgtcgc acgtgcactt cctgatgtca gagatggtct
     3421 gaaacccgtt catcgccgta ttttatacgg catgaacgaa ttaggtgtaa caccggataa
     3481 agcccataaa aaatctgcca gaatcgtcgg agatgtcatg ggtaaatacc atcctcatgg
     3541 agactatgcg atctatgagt caatggtacg gatggcacag ccattcagct accgttatat
     3601 gctagtggat ggtcatggaa actttggttc tgttgacggt gacggtgccg ccgctatgcg
     3661 ttataccgaa gcacggatga gcaaaatcgc tacagaaatg ctgcgtgata tcaataagaa
     3721 cactgttgat ttccaaagca actatgatga tacagaaaaa gaaccagttg tactacctgc
     3781 tcgcttcccg aatcttttag tgaacggaac aacggggatc gccgtaggta tggccacgaa
     3841 tatcccacct cataacttgt ctgaggtagt agcggctatc gatttgctga tggataatcc
     3901 tgatgtgacg accaatgaat tgatggaagt attacctgga ccagattttc caacaggtgg
     3961 tttggttatg ggaaaatcag gaatccgaag agcgtatgaa acaggtaagg gttctattac
     4021 cgtacgtgca aaagttgaga ttactgagat gccaaatggt aaagaacgga tcatcgtgac
     4081 agaattgcct tatatggtca ataaagcaaa attgatcgaa cggatttctg aactgcacag
     4141 agacaagcgt atcgaaggga tcacagattt gcgggacgaa tcttcccgtg aaggtatgcg
     4201 gatcgtcatt gacatccgtc gtgacgccag cgcatctgtt atcttgaata acttatataa
     4261 attgacatcc ttgcaaaatt catttggatt caatatgttg gcgatcgaaa aaggtgtgcc
     4321 gaaagttttg agcttgaaac aaattcttga aaactatatc gaacatcagc gcgaagtcat
     4381 cacaagacgg acagtctttg aaaaagaaaa agcagaagca agggcacaca ttttagaagg
     4441 tttgcgtatt gctttggatc atatcgatga aatcattgcg attatccgcg ggtctaaatc
     4501 agatgacgaa gcaaaagcga cgatgatcga acggttcgat ttatccgatc gccagtctca
     4561 agctatttta gatatgcgtt tacgccgatt gaccggtttg gaacgggaaa aaatcgaaaa
     4621 tgaataccaa gaactcttaa aactgattga agatttaact gacattctgg caagaccaga
     4681 acgtgttaca gaaatcatca agaatgaatt agcagacttg aatcaacgat ttggtgataa
     4741 acgacgcaca gaattactag tcggtgaggt cttaagtctg gaagatgaag atttaatcga
     4801 agaagaagaa atcgttatta cattgacaaa caatggttac atcaaacgtt tggcaaataa
     4861 tgaattcaga gctcaacgaa ggggtggacg tggtgtccaa ggaatgggta tccacaatga
     4921 cgacttcgtc aaaaatctgg tttcttgttc tacccatgat acattgttat tctttacgaa
     4981 taacggaaaa gtgtatcggg caaaaggata cgaaattcca gaatacgggc gcacagccaa
     5041 aggaatacca attatcaatt tactaggtat cgattcgagt gaaagcatcc aggcaatcat
     5101 tgcggtgacg ggagaagcag aagaaggcca ctatctcttc tttacgacgc gtcaaggtac
     5161 agtaaaacgt acttctgtca ctgcttttgc taacatccga agtaatggtc tgatcgcaat
     5221 tggtttgaaa gaagacgatg agctcgtcaa tgtgctactg acagatggtc aatctaatat
     5281 cattattgga acacataacg gctactctgt cactttcaaa gaagaagcag tccgcgatat
     5341 gggacgtaca gcttccgggg ttagaggaat acgcctgcgt gaggaagatt atgtcgttgg
     5401 ggcagcctta atgaaggaag atcaagaagt attagtgatc acagaaaaag gttacggaaa
     5461 acggacaaaa gtatcggaat atccagtgaa aggccgcgga ggtaaaggga tcaaaacggc
     5521 gaacatcact gaaaaaaatg gtccactagc tggattgacc actgtttctg gagatgaaga
     5581 catcatgctg attacagata aaggtgtcat tatccgtttc aacgtatcga ctgtttctca
     5641 aactggacgt tcaacattag gcgtacgttt gatgaagatg gaagctgata cgaaagttgt
     5701 cacgatggca gcagtcgaac cagaagctgc agaacaaaca gttgaagaac aaggatcaga
     5761 agaatagcca tcaagactaa ttgtgtaagc tagcaagaat gaccgattaa aaaatctggt
     5821 tattcctgct ggcttttttg attagttgaa tttctcttaa aatattcgaa actgttcatt
     5881 ctgtattgct tttgaccaaa atagagtgta taatcagagc ttgtgagtag tctaattaat
     5941 tagataactc tccttgctct taggaaccta agagccgaaa gtccacaggg aggtgaatat
     6001 caatggaaaa cacgaaatat gaaatcatgt acattattcg tccaaacatt gatgaagaag
     6061 caaaaactgc tttaatcgaa cgtttcgatg caatcttgaa agataacgga gcagaagtta
     6121 tcgaatcaaa agattgggaa aaacgccgtt tcgcttatga aatgaacggt taccgtgaag
     6181 gtatctatca catcgttaaa gtatcttctc catcatcagc aaatgctgtc aatgaatttg
     6241 accgtcttgc taaaatcaac gacgatatca ttcgtcacat gattgttaaa gaagaagctt
     6301 aattgttgtt tcatgtgaaa caatagaaaa cgatgagagg agatgactat tttgattaat
     6361 aatgttgtat tagttggacg tctgacgaaa gatccagatt tgcgttacac agcaagtggt
     6421 acggcagtcg caacatttac tttggctgtg aatcgtaatt ttacaaacca aagcggcaat
     6481 cgcgaggctg attttatcaa ctgtgtgatt tggcgcaaat cagcagaaac tttagcaaac
     6541 tatgctcgta aaggaacact tttaggtgtt actggacgta tccaaactcg ttcttatgat
     6601 aatcagcaag gacaacgtgt atacgtgaca gaagtagtag ctgacaactt ccaattgctg
     6661 gaaagtcgtt ctgcatctga aaatcgccaa caaagcggcg gattccaaag ttctggtcaa
     6721 tcagctggcg gatttggcgg aaacaataac tctaatcaaa catctcaatc atccaacggt
     6781 atgcctgatt tcgaccgtga tacttctgac ccattcggct caagttcaac gatcgacatt
     6841 tcagacgatg atttaccatt ctaaacgaat ttgataggag ggaaatagaa tggcacaaca
     6901 aagaagaggc ggacgtaaac gtcgtaaagt agactacatt gctgctaacc atattgaata
     6961 tatcgattat aaagatatcg aattactaaa aagatttatt tcagaacgcg gtaaaatctt
     7021 gccacgtcgt gtaactggta caggcgctaa aaaccaacgt aaaattactg ttgcaatcaa
     7081 acgcgctcgt atcatgggct tgctaccttt cgttagcgaa gactaataaa tcctaggaat
     7141 tgaatgactg atccttttga tcagctattc aattcctttt tttctgtaaa aataacctct
     7201 attcgtttag tcgctcgaat gacgaaagtt atggtaaaat caaattagtc atatatggga
     7261 ggaaaattat ggaaaataaa aacaatcatt tatccccgaa acctctcttc cttatcctgt
     7321 ttatattaat cgaagtattg gtagtcttaa ttttaccatt tcgttttgtt agcgcttcgg
     7381 tcattctatt gatgaattta tggggtgttc gctattggtt gaaaatgaaa cgttggctta
     7441 ttttaaataa acgtgaaaaa atccagcagg cttcgactgt agctgaaacc aatctagctt
     7501 atgtctcaga agtcatgcct gtaggagtga ttgcttatca gccatcaagc caaaaaatcg
     7561 aatggctcaa tccattagca ttgaaaatca aagatcaaag tggattaaca gaggaagaat
     7621 tgctagatac tttttttact gcaaaagaaa aaaagaaaaa ccaagtctat ctagcagata
     7681 aaacatttca gtttgatatg gatacagtaa agggtgttgt ttattttaat gatattaccc
     7741 aaaaccagtt gttaaaactc catcagacga acagtcagcc ggtgattggt attttatcta
     7801 ttgataacta tgcagatgcc attgataaat tggatgacaa agagatttct tatttgaact
     7861 ctttcgtcac tacactagtt tctgactgga tgaatgaata ccatgtcttt ttcaaaagaa
     7921 tcaacgcaga acggtttttc ttcgtagcac agatgatcga tctgaaaaag atgatggata
     7981 aaaaatttga tttgctggat cgctttcgag cagaagctga aaaacagagc tcgcctatca
     8041 cccttagtat ggggattgct tatggctcaa gaaccctcga tgaaatcggt ggagaagctc
     8101 agaataattt agatgttgct ttagtccgtg gaggcgacca agttgtcgtg aaagaggtaa
     8161 aagaaggagc aaaacctgtt tattatgggg ggaaatctgc taagaatgtc aaacgcacgc
     8221 gtgttcgttc tcgagcgatg agtaccgctt tgcgtcggat ttttgatgaa acaggcgatg
     8281 tgtttatcat ggggcacaag tttccggata tggatgcttt aggctctgct tttggtgtca
     8341 gttgtctggc acgcttcaac cagaaaaaag cttacatcgt tatcaacgag aacgagatca
     8401 ttccagatgt agaacggtgt cttgaagaaa tccataagca tcctgaatta gaaatgcaac
     8461 taatctctcc agaacgtgcc atagagctga aaaaagaaga ggacctcctc gtgatggtgg
     8521 attaccataa accttctcta tcgatctctc agggcctata tgagcaattt gataagattg
     8581 tgatcattga tcaccatcgt cgtggggaag agtttccaag taaacctctg ctgacttata
     8641 tcgaatcttc agcttcttca gcttcagagc tagtttctga gctgatccaa taccaaagca
     8701 gcaaaacaaa tcgcttagat cgttttgagg ccacattatt gcttgcaggg atggttgtag
     8761 atacaaatag tttcaatgtc cgtacaactg cacgaacttt tgacgttgcc agctatttaa
     8821 ggtcttgtgg agcagacgca tccttagtac aatatttatt gagctcggat ttagcgacat
     8881 acttggagat cagcaatttg attgctggaa gcgactatgt gacttctgat attgttgtgg
     8941 cggcagggac agacgaaaaa gagtataata gtgttatagc agcaaaaaca gcagatacat
     9001 tgctttcgat gatcaatatc aatgcagctt tcgtcattac aaagcggaca gacggcttga
     9061 ttgggatcag tgccagaagt accggcacga tcaacgtcca aatcatcatg gaatccttag
     9121 gtggcggagg acacttcaca aacgcagcta ctcaactgga aaatacgaca gttgaggaaa
     9181 caaaacagaa gctatttgct gcaatcaaag aaaatatgaa tcaaatatat gataaggagt
     9241 aatccaaatg aaagtaattt ttttacaaga tgtaaaagga aaaggtaaaa aaggggacat
     9301 caaagaagtt ccagcaggtt atgcacaaaa ttatctaatc aaaaataact tggcaaaaga
     9361 agcaacgaaa agcagtatca gtcagttagc aggacaaaag aaagcacaag aaaaacacga
     9421 agctgaaatc ttggaagaag ctaaaaagtt aaaagaattc ttggaaaaag aagatactgt
     9481 agtcgaattg aaggcgaaag caggggaaga cagccgtttg tttggttcga tcccttcaaa
     9541 acaaattgct gaagggttaa acaaacaata caaaatcaaa ttagataaac gaaaaatcga
     9601 attagaaaat ccgatccgtt cactaggcta cacaaacgta cctgttaagc ttcatcatga
     9661 ggtgacggct acgatcaaag ttcatgtcgt acaagaataa caaatcttga tagcgatgtt
     9721 cagctggtaa gtataaccag aaggaaaaaa gacaaaccga aatttatctg agaaaacagc
     9781 tgcagcaatg aaagcaaaag ctgcaaatcg atttcagatt gatttcggtt tcttttttgg
     9841 gaacagcaaa atagcataaa gaaaacgagt ataataaaag agaactattt ttttattaca
     9901 ctttcggtac gaatgatgac ttttctctca ttttcttgta aaatgaagca attgaagaca
     9961 aggagcgata cgtatgaatg aagtgtggca ggatcgaatc ccaccgcaaa atattgaagc
    10021 tgaacaagcg gttttaggct ctgtttttct tgacgcagaa acgatcattg atgccatgga
    10081 gtacatagag ccaaaagact tttatcgtcg tggccatcaa attatttttg agacaatgat
    10141 cgagttaaat gatcgtaatg aagcgattga cgtggttact gtcaaagacc ggctggaaca
    10201 agccaatttg ttagaagatg ccggtggttt aagttatctc tcagacttgg cattagcagt
    10261 tccgacagca gcaaatatcg tctactatgc gaaaatcgtt gaagaaaaat cattgctgcg
    10321 tacattgatc cagacagcaa caggaatcgt tacgaaagga ttcgagcaag gagaagaagt
    10381 tcaaacaatt ttagatgatg ccgaacgcag tatcttggag gtttcggaaa aaagaaatcg
    10441 aagcggcttt ttatctattg ctgaagtact gaactccacg attgaaaata tcgatcgact
    10501 agctcaaaac aatgaagaaa tcacaggtct accaactggc tatcaaggtc tggataaaat
    10561 gacagcaggt ttccagcccg aagaactaat cattcttgct gcacgtccag cggtaggtaa
    10621 aacagcgttt gcattgaata ttgctcaaaa tgtaggaaca aaaacagatc gttctgttgc
    10681 gatatttagt ttggaaatgg gggcagaatc tcttgtaaat cggatgcttt gtgcagaagg
    10741 ttcgattgag gccagccatt tacgtacagg tcagctttcg gaagaagaat ggtcgaatct
    10801 gatcgttgcg atggggagtc tttctcgagc aaatattttt atcgatgata caccagggat
    10861 caaaatctct gaaatccgtg cgaaatgtcg gaaactagcg caagaaaaag gcaatctagg
    10921 cttgatattg atcgattatt tgcagttgat tgaaggtaac ggtaaagaga accgtcaaca
    10981 agaagtatca gatatctctc gccaactgaa aaaactggcg aaagagctga aagtacctgt
    11041 cattgctctt tcacagttat ctcgtggagt agaacagcga caagacaaac gaccagtgct
    11101 aagtgatatt cgtgaatccg gatcaattga acaagatgcg gacatcgtag ccttcttgta
    11161 tcgagatgat tattatgaaa gagaaggcgg agatgacgat gatggaaacg agccaaaaaa
    11221 tgacaacgtc atcgaagtca tcatcgagaa gaaccgaagc ggtgcacgag gaacagttga
    11281 actactgttt atcaaagaat acaataagtt ttcgtcgctt tcgccaagag aagaatttta
    11341 aagaagaggt tgtgagcctt gagcaatccc gagagctctc gtaagaagat gcaaggagta
    11401 accgcaggaa cgactagagt acttaattta aacagatctc caaagggatt gtgacaaaag
    11461 tcttgtcaca gtcccttttt ccgaataaac ggtgttcaat cagctgaccc ttggtattag
    11521 ctcagcaaac gtcaaaatat gaggaactat tttgacgttt gtttctctaa tactcaggtc
    11581 aaaacgctga tcttacgacc tcttttcctt aaacggtgtt ctcctgccaa ctcctcggtc
    11641 acaagtcaca atatcatcaa acatgagaag cagttttttg aaattgctcc ttgtgactca
    11701 gagttaaacg gcaggctcac aacctctttc ttaaacggtg ttcaaaagct tgcatctgcg
    11761 gtaatacgcc caatcaaacg aaaaatatga gaagctattt ttgtttggct gttcttatta
    11821 cttgatgcag gacagctttt tcacaacctc tgattcacgg tgttccaaaa gtagcttctt
    11881 cgaaaaataa gtcgaaattt cacaaaaatt tgagaagcaa ttttcgtaca ttccttctta
    11941 tttcttgaag ctgaccactt ctgttacaac ctctttttta ataaccattc taaaaagata
    12001 aagttcgttt tttaagcgtt tttataaaat aaaaataaaa atcattcgat tttatttgaa
    12061 atatgtatct attattgata caatggttca ggattaaata atatagaacg aggtgttcac
    12121 atgtcatctg tagtagtagt aggtacgcaa tggggcgatg aagggaaagg aaaaatcaca
    12181 gactttctca gtgaaaatgc tgaagtgatt gcacggtacc aaggtggcga caatgcaggg
    12241 cacacaatta agtttaacgg tgtgacttat aaactgcatt tgattccttc tgggattttt
    12301 tataaagaca agatcagtgt gatcggaaac ggcgttgtag tcaatcctaa atcattggtt
    12361 aaagaattag cttatttaca tgaaaaaggt gtagaaacaa gtaatttgcg tatttctgac
    12421 cgcgcgcacg taatcttgcc ttatcatatc aaattggacc aattacaaga agatgctaag
    12481 ggcgaaaata agattggtac aacgattaaa ggaatcggac ctgcctatat ggataaagca
    12541 gctcgtgtag gtatccgtat tgcagactta ttagataaag aaatttttga agaacgtctt
    12601 cgtttgaatc tagatgacaa aaaccgtcaa tttgtaaaaa tgtttgattc agaaccactt
    12661 gattttgacg atatttttga agaatattat gaatacggac aacaaatcaa acaatacgtc
    12721 acagatactt ccgttatctt gaatgatgct ttggatgcag gcaaacgcgt gttgtttgaa
    12781 ggcgcacaag gtgtaatgct ggatatcgac cagggaactt atccttttgt tacttcttct
    12841 aacccagtag ccggtggggt aacaatcgga agcggtgtag ggccttcaaa aatcaataaa
    12901 gttgtcggcg tctgcaaagc ctatacgtca cgtgtcggtg atggaccatt tccaactgaa
    12961 ttatttgatg aaacaggaga acaaatccgc gaagtaggac gagaatacgg tacgacaact
    13021 ggtcgtccgc gtcgtgtagg ctggtttgat tctgtagtta tgcgccattc taaacgagtt
    13081 tcaggtatta ccaacctatc tttaaactcg atcgatgtat taagtggatt ggaaacagta
    13141 aaaatttgta cggcttatga attagatggc gaactaatct accactatcc agcaagcttg
    13201 aaagagttga accgctgcaa accaatctat gaagaattgc caggctggtc agaagacatt
    13261 acaggatgca aaacacttgc agaactgcca gaaaacgcac gtaactatgt acgtcgtatc
    13321 tcagagcttg ttggtgtacg tatctcaaca ttttctgtcg gacctgatag aacacaaaca
    13381 aacatcttag aaagcgtctg ggcgcaaatc taaggataaa aacgacgact gaatcaggac
    13441 gttcttacca gagcgagtcg aagtgtttat ctatacgata ataataactt gaacactgtt
    13501 tattcagaat aagggactgt gacaaaaacc gtgtcacagt ccctttttcc gaacaaacgg
    13561 tgttcaaaag cttgcatcta cggcactaag cccaatcaga tgaaaaatat ggagtgctat
    13621 ttttatctga ttgttcttac tgcttgatgc agagcagctt tttcacaacc tctccactaa
    13681 acggtgttct cctgccaact cctcggtcac aagtcacaat attcaacaaa catgagaagc
    13741 tgttttttga aattgttcct tgtgactcgg agctaaacgg caggctcaca acctcttggt
    13801 aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa atatgaggaa
    13861 ctattttttc cgtttttttc tctaatactc aggtcaaaac gctgatttca caacctctga
    13921 ttcacggtgt tccagaagta gcttctacgg aaataagccg aaatttcaca aaaatttgag
    13981 aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctct
    14041 tattttgatt agaacttgcg aaaataaacc ggttaaagtg tgtctagcgc aagagatttg
    14101 ctataataga cgataagggt aaaatgtaac gagaaggggt tgaaaaatgt gtatcaaatt
    14161 gtaacagatt cttgttgtga tctgccatac catgtattag caaaagaaaa agttgatttt
    14221 ttatcaatga atctccaatt gaatgatcaa gagttagtag atgacctcgg aaagacattc
    14281 aattatgacc gctacatgaa tcagctgaaa aatggtgcga tgcctacgac atcacaaatc
    14341 aatgttggcc gatatttaga atttttccgt tcttatgcgg aaaaacagat tcctgtttta
    14401 tatgtggctt tttccagtgg attaagcggc tcttacagta gtgcgcttca agcggttgaa
    14461 ttgctgaagg aagaatatga agaccctgag atctatgttt ttgatacaaa agctgcaagt
    14521 attggggaag ggcttttagt attggaagct gtaagattga aaaaagaagg taaaagcatc
    14581 gaagaaaccc tagagtggct ggaaagtaat tatttgaaag tccattcatg ggtaacagta
    14641 gacgatctga aatatctaga acgaggcggt aggatttcca aagcaagtgc tactcttggc
    14701 agtctattga gtgtaaagcc tattattacg gtggataaag aaggaaggct gacgaatgtt
    14761 gacaaagttc gaggaagaaa caagtcgatc cagacaatcg tcaatgagac ggtaaaaaca
    14821 atcgttgcac cacttgatca aatcgtttat gtggcttacg ctggggatct agagagcgcg
    14881 gagaaggcaa aacggctttt ggaagagcag ataaagatga aagatgtgaa gctttatccc
    14941 ttaggaccaa cgatcgccag tcacacaggc tatggctgta tcgcgatttt ttcgatggga
    15001 atcagtcgat aaaagcagta actcattctg taaaaaagcc cagaaattga ttgtaacaaa
    15061 aacagcgact cttaaaagat agatgagcat cctatctctt agaggtcgct gtttttttat
    15121 tttttaagct aaataggcca ttatgcccat aactcgttca attgtttttg aatatctgca
    15181 ttttccaaaa attcatcata tgtcgtatca gctcgatcga tcacaccttt atccgaaacg
    15241 gcaatgatgc ggtttgccaa tgtttggata aattgatggt catgtgacgc aaataagatt
    15301 gagccagtaa atgccatcaa gccgtcattc aatgctgtga ttgattctaa gtctaaatgg
    15361 ttcgttggat catctaatac aagtacgtta gctttcgaaa gcatcatttt tgaaagcatg
    15421 caacggactt tctctccacc agataaaaca ttcactggtt tcaatacttc ttcaccagaa
    15481 aacaacattc ttcctaagaa gcttcttaag aaggtgttat catcttcttc tttacttgca
    15541 tattgacgca accagtctaa aatcgttaga tcagtatcga attctttcgt tgtatccttt
    15601 ggtaaataag aattacttgt tgtcacaccc caacgtactg ttccagtatc tggcgtaatt
    15661 tcacccatga taactttgaa tagcgttgtt gttgttatat cattttctgc gacgaaagcg
    15721 accttgtctt ctttgctcaa attaaaagat atatcatcta aaattttctt tccatcaatc
    15781 gttacactta cattttctac ctgcaataag tcgtttccaa tctcacgttc aggtttgaat
    15841 ccaacgaatg gataacgtct agaagaaggt tggatatcgt ctaaagtaat tttttcaagc
    15901 atttttttac gtgaagttgc ttgttttgat tttgatgcat tcgcactaaa gcgggcaata
    15961 aattcttgga gttctttgat ttgttcttct tttttggcat ttgcgtttgc ttgaagttta
    16021 gccgctagtt gactagattc caaccaaaaa tcataatttc ctacataaag tttgatttta
    16081 ccaaaatcta gatccgccat atgtgtacat actttgttca aaaagtgacg gtcatgggat
    16141 acaacgatca cagtattttc aaaattgatc aaaaattctt ccagccaatt gatcgattgg
    16201 atatccagac cgtttgttgg ttcgtctagc aaaagaacat caggtttacc aaatagtgtt
    16261 tgtgctaata acacttttac cttttgtcca ccagttaatt cactcatttg ctgatggtgc
    16321 aattcttcag ggatgttcag accttgcagt aaaacagctg cttctggttc agcttcccat
    16381 ccattaagct cagcaaattc tccttctaat tcagctgctc gaataccatc ttcgtcagtg
    16441 aaatcctctt tcatgtaaat cgcatctttt tctttcagca cttcatacag acgcttgtgt
    16501 cccatgatga ctgtttcaag tacagtatag tcttcatagt cgtaatggtt ctgtttcaat
    16561 acggccatcc gttcatctgg tcccatagta acggaacctg tggaaggctg cagatcccct
    16621 gataacactt taaggaacgt tgatttacct gcaccatttg caccaatcaa gccatagcag
    16681 tttccggggg taaatttaat attcacatca tcaaatagtt tgcggtctga aaagtgcaaa
    16741 ctcacatcat ttacagtaat caattttttt cctcactttt atcattatta gttttgacaa
    16801 gtcttataga attatatagg ggaaaggtaa aagattcaag gcaatcgaac attctttgtg
    16861 tttcacgtga aacagaatga aaaaaataaa aataaaagcg gagcgaaaat agctactata
    16921 ggatttatta gtttgcaaca gcataatttt tgacgttatt tataagaggg agtactataa
    16981 aactgtaaaa gaaatgaaag aaggattgaa aatggatgaa aaaagaacca tcgactgggg
    17041 ctcattgatt ttgggaattt tatttgtact tgtctcattg ttatcatttc aagaccctgt
    17101 cggaaattta gttgcaattg tagtagtctt tgcgattttt gcctttatta aagggatatt
    17161 tgaactgttt gtacggaaca gattaaaaga gctaacagga tataaaggaa agatgccctt
    17221 aataatcggg atcatcgata ttctagttgg cgtctttttc ttattcaata ttggtgcagg
    17281 agtcgcagca ttgccttttg tttttgcagt atggtttatt gctgattcag ttttagcgtt
    17341 atttactgct gacttggcaa gaggagtaag tgaaggatat tattggttta cgatcatcgt
    17401 caatatttta ggaattttat taggaatttt cttattgttt aatccaattt cttctgcgtt
    17461 gacattaagc tttttagttg gattctattt tatgttattt ggtatcacac atatcgtcta
    17521 tgcatttaga taagtaaaat caaacaaata aaaaagccgg cttccgatta cagttttact
    17581 gttgtaattg gaagccggct tttttatcat ggacaaatat gcacgaggtg atttttaggt
    17641 gtcgagacaa atttatgcaa aaatgtgaga agctatatac acaaatttta tcttaatact
    17701 cgaagcatag cgcagttgtc acaacctctt ttcatataat tcattcaata ctggcaaatc
    17761 atggacttca tatttttctt tgaatgcttg gatttcttct tcagaatatt ttcttggatc
    17821 taaatgcaga tcttctactg gcaatgggcg cttgttattg atcttcacat cgattactac
    17881 aggacgatct gattttgcag ctgcatcaaa ggcaaatcga agttgcgagt attctgtgac
    17941 tgtaaagcta tttgccccga gcgcttctcc aactttaccg aaatctgcat cttctaaaaa
    18001 tacaccaaat ttcttttgtt ccgtatcttc ttgttctgct tcgatgaaac caaaagaatc
    18061 attcgaaaag acaacattaa taattggaag ctgatatttg acttgtgtaa tgatatcttg
    18121 catgaccata gcaaacccgc catcaccact taaggtaaat acttgttttt ctgggtagct
    18181 tagttgtgcg gcaattcctc ctggcacacc attacccata gttgcgaacc atccagatgt
    18241 cgtatgtttt tgtttaccgt tcatattcaa taaacgaata gaatggatag tcgtgttacc
    18301 gacatctgtg acgaaaatcg catcgtcttc agcaatacga ttgatttctt tatagacagc
    18361 ctctggacga atcggttctt cctctcgatc ttcaaaagat ttcaaccaat ttacccattt
    18421 ttctttattt tcttgatttg ctaggtacca gctatcagca gggcgagcat cccctaactc
    18481 gacaagtctt tgcaaagctg tgtttgcatc accgaggatc gacaaagatg tttcatggcg
    18541 tcgtccaaat tttgacgcat cgatatcgat ttggataaat tcagcagttg gattaaaaaa
    18601 tgcacgtcca aatgggaagt cgctaccgac aaataagatc agatcggctt ctgccaatgc
    18661 ttcatttccg ggtttagtag cgacacgtcc agcaaaacca agaaagtttt cataatcatc
    18721 aggaataatt ccttttgcta gaacggaagc gacaacagga actgaaaaat gttctgagaa
    18781 ttctttgatt tgcggaaatc cgtttcgcgt tccttgacca acataaagaa ctggtttttt
    18841 tgctttttca agataaggca aagcagctag caaatctttt tcttctggta atatcacact
    18901 tgttttgtga gtgtgagcgg tggagaaagg ttgttcgtca atttcttcaa aaccaagatc
    18961 aacagggata gtcacgatag ccacgccttt atgctcatag gcggctttaa tggcttcatc
    19021 tactacatgc ggaagacttt gtggtgtcat gactgtacga ttgtatacgc taacatctgc
    19081 gaaaatagga ttttcattta gttcctggaa agagttatag ttcatggatg tagaagcaac
    19141 ttgtcctaag aacgctacta gaggcacatg gtccatctgt gcatcataca aaccattgat
    19201 caaatgtgtc gcaccaggtc ctgcagagcc gaagacagcg ccgactttcc ccgttaattt
    19261 tgcatctgca gcggctgcga gtgcgccgac ttcttcatga cgaacttgaa tgtagttgat
    19321 ttcggttttt tcttgatata gcgcatccat aattgaattg aaagatcctc cgggaatgcc
    19381 gtaaatatgg tcgatacccc agctttcaag aacttttacc attgcgattc ctgcattgat
    19441 ttttgacatg gtgtttcact cctttagcaa tgatgataca taaatttttt gtatctagag
    19501 tatagaactt tttatcgaaa atcccaacta attcgtcttt tcacaaaaat gtagtcatgt
    19561 ttttgggaat atgctagagt agtacataga tagattcaag agaggggaat ggcatggttg
    19621 aatggctatt gagacttgaa ccttggcagc aagcacttgc tgggacagct tttacttatt
    19681 ttatgacagc tcttggtgcc gggttggttt ttttctttaa agaaatcaaa aaagaagtgc
    19741 taaacttgat gttaggattt gcttctggtg tcatgattgc tgcaagtttt tggtctttgt
    19801 tagatccggc tattacaaaa gcagaagaaa atggagatat tgcttggcta gttgtaagta
    19861 ttgggtttgg tttaggcggt gtgtttttat atatggcaga taaaacactg ccccatatgc
    19921 attttggacc acaacatgaa gcagaaggat tgcctaccca tttaaaacgg acgatcttgc
    19981 ttgttttttc tattacattg cataacattc ctgaaggttt agccgtgggg gtagcattcg
    20041 gtgcagcagc aactgcggat aatccgacag ccgctatatt agccgctgtt tcggtggcgc
    20101 taggtattgg tatccaaaac ttcccagaag gagcagctgt atcaatcccg ttgcgacaag
    20161 aaggtctgag tcgtaaaaag gcctttgttt acggacaagc ttctggaatc gttgaaccaa
    20221 ttgctggagt gattggagcg ctactagtaa cgaaagtgga gctgctgctt ccttatgcac
    20281 ttgcttttgc cgcaggagca atgatttatg tcgtggtgga agaattgatc cctgaagcac
    20341 agcagacatt gtccagcaaa cgtcattttg cagtttttgg cgtcatgagt ggatttatca
    20401 ttatgatgat tttggatgtg gcattaggat aaataaaaaa aatatatatg atatatcaaa
    20461 tagggccgcg aacttatatc gcgaccctat ttttttagta agtt
//
LOCUS       DACDMM010000039        20311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDMM010000039 DACDMM010000000
VERSION     DACDMM010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.039"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(24..551)
                     /locus_tag="IWD16_001078"
     CDS             complement(24..551)
                     /locus_tag="IWD16_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9088158.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(618..2120)
                     /locus_tag="IWD16_001079"
     CDS             complement(618..2120)
                     /locus_tag="IWD16_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9088159.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2378..3499)
                     /locus_tag="IWD16_001080"
     CDS             complement(2378..3499)
                     /locus_tag="IWD16_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9088160.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3625..4566
                     /locus_tag="IWD16_001081"
     CDS             3625..4566
                     /locus_tag="IWD16_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9088161.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4716..5612)
                     /locus_tag="IWD16_001082"
     CDS             complement(4716..5612)
                     /locus_tag="IWD16_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9088162.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5881..7221
                     /locus_tag="IWD16_001083"
     CDS             5881..7221
                     /locus_tag="IWD16_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9088163.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7439..7882
                     /locus_tag="IWD16_001084"
     CDS             7439..7882
                     /locus_tag="IWD16_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP9088164.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8386..9804)
                     /gene="nhaC"
                     /locus_tag="IWD16_001085"
     CDS             complement(8386..9804)
                     /gene="nhaC"
                     /locus_tag="IWD16_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9088165.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10207..11430)
                     /locus_tag="IWD16_001086"
     CDS             complement(10207..11430)
                     /locus_tag="IWD16_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP9088166.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11461..12300)
                     /locus_tag="IWD16_001087"
     CDS             complement(11461..12300)
                     /locus_tag="IWD16_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9088167.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12317..12997)
                     /locus_tag="IWD16_001088"
     CDS             complement(12317..12997)
                     /locus_tag="IWD16_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9088168.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(12999..14072)
                     /locus_tag="IWD16_001089"
     CDS             complement(12999..14072)
                     /locus_tag="IWD16_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9088169.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(14719..15390)
                     /locus_tag="IWD16_001090"
     CDS             complement(14719..15390)
                     /locus_tag="IWD16_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9088170.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(15477..18155)
                     /gene="mgtA"
                     /locus_tag="IWD16_001091"
     CDS             complement(15477..18155)
                     /gene="mgtA"
                     /locus_tag="IWD16_001091"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP9088171.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            18814..19251
                     /gene="ndk"
                     /locus_tag="IWD16_001092"
     CDS             18814..19251
                     /gene="ndk"
                     /locus_tag="IWD16_001092"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP9088172.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19273..20139)
                     /locus_tag="IWD16_001093"
     CDS             complement(19273..20139)
                     /locus_tag="IWD16_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9088173.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
BASE COUNT         5824 a         4235 c         3585 g         6667 t
ORIGIN      
        1 atttaaataa cacaaaaaca taattactca taagaaacca attctgtatt atctaccatg
       61 aatgtaaaga acccctcatt aggcatcgga tgaaaataag cattgatcac tcttatcaag
      121 gcattatggc aaactaacag atagacattt tcttcgtctt cgatgcattc tttcaataaa
      181 ggaacgatcc gtgcatacac atctaagact gactccccat ttgggaatcg tacagcgaac
      241 gctaaacgtg ctttttgaaa gttctcatct ttgcttggca gtccatcata atccccaaaa
      301 tccatttcta ccaatcgttc atcaacggtc agcggcaagc tgagacgatc agcaactgct
      361 tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga ttttcgtgat tggaacaggc
      421 aaatctacta ccttttctgc tagtttctgt gcctgcatct gtcctttttc agtcagcggc
      481 acatctgccc gtccgcaaac taatccttgt gcgttccaag ttgtttctcc gtgtctcgtt
      541 acataaagca tgtgattctc tccttcgttg gcaaagttcc ccttaaccat accttgttgt
      601 tcttgagatt acaagggtta gattctctca agagaaataa tctcagtaga tactttatcc
      661 aggaaggttt gatcatgttc cactagtaac atggcaggtt tcacgttcaa gatcagttgt
      721 tctaattgtt cttgattgaa gacatccaaa taattcaatg gttcatccca tgtatatagt
      781 tcagctggct gtgataaaga tttagccaat tccacttttt tccgttggcc catactcatc
      841 tgctcgatct tgttatgaaa aacatctctt tccatcccaa gctttcggag gttgttcaaa
      901 aatgcttggt agtctacttg gtttttctct gcaaattccg ccaacgttcc tcgattgtct
      961 tcataatttt ggcttgcata actaatgctc agatgttttg gctggtattt ttctcctata
     1021 accttgccgt tgaatgcccc cagaagataa tggataatgg atgactttcc tgcaccgtta
     1081 ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa aatgaattgg ctcgaataac
     1141 agattttcat accctaattg aagatcttct acgcttaaaa gtcgcttatg gtgagacgct
     1201 tggctattca tcgtcaaaga atcgatatat tcgatatctt ttaatagttt ttctttctcg
     1261 ctgatctggg tctccatccg attcacgatt gctttggatc gtttcatcgt ccgtgcagca
     1321 tcagcaccca ctgctccttt attcactcgt ctagattcag tatcgatgaa tccgacttgt
     1381 ttctttgttt tatctccttc tcgggaacga gaccattcgg ctttttcagc tgctgttttc
     1441 tttagcctac tgacttcttt tttcaatttt tcattttgag ccatttcaaa ttcatcacga
     1501 agttttttct gttcttcata gatagagaaa ttcccttgat aaagttccag ttgacttttt
     1561 tcgattgcta aaacatggtc cactacttca tcgataaatc cccggtcatg gctgaccacg
     1621 atgaagcctt gttttttctt tttcaaataa gccgctactt gttttctacc agagatatcc
     1681 aaatgattcg ttggttcatc gattaacggg aaatgagtgt catccacaaa taaaagtgcc
     1741 agtaggactt ttgtcttctc tccccccgat agtgtgctga attctctcca taagatttca
     1801 ggatctgttt gcatcaattg gagctctctt tcgatttccc atatctcaaa atccgtaata
     1861 tcatttaaca cgtaataggt taaacgttct ttatcttttg tctgttgcgg gaaataggca
     1921 aattcttgct gatggattac ttgcccttga taaggtagtt tgttttgtag aatattcagt
     1981 aaagttgtct ttcctcgacc gtttcgtccg ataagtccta gtttccattg tgtgtcaaaa
     2041 tttagatttg cttgttcaaa taataatgtg ccttggctgt cgtagccgaa tgtcagattt
     2101 tttatttcga ttttagacat tttccatcac ctcgtaaaaa aagaccagtc ctattttcag
     2161 aactggctaa agaggatgca agggttggat acacacaaaa agacccatac atcgaaaaaa
     2221 ccaagccaat cccgaaaatc tgcacactgc ttccttgtct ccacaaggta cagtctgaat
     2281 gcagctttca tcaatcgact taatttttca atgcacggat ccctcacttt gtcctatatt
     2341 tgaaattacg atagcataaa gaaaatgatt ttgtcaacta cttcgggagg atggtgaaaa
     2401 atctgctaag tgtctgtcct aagatttttg ttttttctaa tgtttgcctg tgttccattg
     2461 cttcttctaa acctattgga cccaaatgaa tacttaagac aattgggaga aaatcagaaa
     2521 gtccttctag ctcttggtct ctacttccgc aaatagcgat tattggtttt cgatattttt
     2581 ttgcaagcac tgctacaccg tatggaactt ttccgtggat cgtttgctga tcgatccgcc
     2641 cttctcctgt aatgatgaca tctgcttgtt cgatcttttt ttccaatcca gtgatttggc
     2701 tgactaaatc gaaaccagaa accatcttcg ctcccaacaa aaaaagggct ccaccaatac
     2761 cgcctgctgc tccacttcct ggaatatcat ctaaatccac ctcccagaaa tcttttattt
     2821 gctgagcaat ctttcctgct tgtttatcta agaaagctag cgtcgtatct attcctcctt
     2881 tttgtcttcc aaattgccaa gcagctcctt gttgacctgt atatggattg gtaacatctg
     2941 cagcgataat caattttact ttagaaacaa gaacgtttct tcgatcaaaa tgaagttttt
     3001 tagtagataa taaaggattt ccttctgtgt acccttctaa agaagcaccc aaggattgga
     3061 gtaatcctag accaccatcg ctacacccgc ttcctcctaa agtgacaata atttgttcta
     3121 cttcttgaag cagtgcatct ttaataagtt ctcctaaggc ataactggaa gcaagtcgga
     3181 tagtttgatc cgttggttta tcaagtagat ctaaccccaa tgcttgtgca gattcgatga
     3241 ctgcggtgtt ttttccttgc caagttgtca ataaatagct gatttttcgc tgacggaata
     3301 agagatccat cccatcaatg gtacgccatg ttcccctgag cgcgtaatag attgcttctg
     3361 cagtattttc tccgccatct gcaatcggca cattgatttt tcttccttta cgatcttccc
     3421 agccatttag aactgcctga tttagttcaa atgaagaagc acttccttta aatgaatcaa
     3481 ttgccgccaa aatatccatc tgtatccctc actttcttct taagtaagac actttttttc
     3541 atttttttca aacaaaacaa tgctattttc ttccattttt ctatatacta aaagggaact
     3601 cattgaataa aggagagaaa aaacatgaaa atcgcaatag ctggggccgg agcaatgggc
     3661 agccgaatcg gattgatgct acatcaaagc ggaaatgagg tcctcttgat cgatcgttgg
     3721 cctgctcaca ttgaagccat tcgtacaaat gggttgattg ctgactttaa cggtaaagaa
     3781 gtggtcgcaa aactgccaat ctattcacca gaagaaatca tcgaaagcaa cgaacacgtg
     3841 gacttgatcg tagcattaac aaaagccaat caattagatg atatgttttg ttcgatccaa
     3901 tccattatta ctgacaatac ttatgttttg tgtttactaa atggattagg tcatgaagat
     3961 gtactggaaa aatatgtgcc aaagaaaaat atcttatttg ggatcacgat gtggacagct
     4021 ggtctagctg gtcccggaaa agtcacattg ttaggtgatg gagaaatcga actagagaac
     4081 ttggaaccag aaggcgaagc atttacaaaa aaagttgtag aagtctttca agaagcaaat
     4141 ttaaatccga tctatagcca taacgttcgt tattccattt ggcgaaaagc ttgtgtaaat
     4201 ggaacattga atggtttatg cacgattttg gattgtaata ttgctgaact aggcgcgcaa
     4261 aaagcggcag agagcatggt tcgaacaatc gtatctgaat ttgcttctat cgcagcaaaa
     4321 gaaggaatca tactagatca agaagaagtg taccaacata ttgcttctac ttatgatcct
     4381 gacaacatcg ggttgcatta cccatctatg taccaagacc tgatcaaaaa ccatcgtttg
     4441 actgaaatcg actacatcaa tggtgccatt tggcgaaaag gacaaaaata tgatattgct
     4501 acaccatatt gtgccttttt gacccaatta gtccatgcaa aagaaggaat ccttggcgca
     4561 gaataaaagg ctctctgatg agaatcagct gaatcagcag ctggttctct ttttttaagc
     4621 ccaataaaag atcaaaaaga aaacacaact ctcatgatgc cataagaatt gtgcttgttt
     4681 gtttcagtac ataatctctt caaaatgtag gttttttatt tgttttcaac agaggagagg
     4741 tcatattcgt attgctcata aatagtggaa tcgttagtga taaatcgttt tgcccaattt
     4801 tctgcatcat aaaattccaa tagctctgat aggattggct taaatggtgt atgcagaaat
     4861 tccggtaatt catctaaatc acgttctcga agcaaaaaaa tattttcagg acgataaaaa
     4921 tgataatgag caatcgcttg attatccgtc actagtttct tttgaaaata catggattca
     4981 aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca gttccagaat cccgcgcgat
     5041 tcccctgttt ttttcaggta ctcttcataa gtaagatatc caggttgttc attccctcga
     5101 tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta taaattcctg cttatagctt
     5161 tctgctcgtt cttttctccc attattcgtt gcaccaaaaa atagatccgt ctttggttcg
     5221 ctagaagaaa aattcatatg tttcgaataa aatgaactat tatgtttcag cccaagttct
     5281 tttgcttcca aaaaatcaaa atgataaatc tcatcgattg ccggttcatc ttgtgcctgc
     5341 ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa aaaataacac aatttttgca
     5401 ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa atccataatc taaaaaaacg
     5461 atcagctgat aatttgccaa ctgttgtcta gctgatttaa tgaattccga ctgtagtaaa
     5521 tcttttccgg accaaacatc taaagagcgg atggaaggca aaaaataaag tgatttattt
     5581 tttgcaacta gcagtacgtc atttttgttc atcttttccg cctcactttc tctgtcactt
     5641 tttcctatga tagcctgttt aaatagtaaa aagccagtaa aacgtttcct atatttgtat
     5701 gaaaatttaa catttgtatg aaaatatgat gaaataaatc attcttctat taaaattcgc
     5761 ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg ttatcattag tcattgttaa
     5821 atattgctat ttattcttaa tataaatttt gattgtgaat ttaaaagaaa gaggtcattt
     5881 atggaagata aaaaattgaa aaaacaactt agctccagac atatcaccat gttagcttta
     5941 ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg aagcgattgg gatcgccggc
     6001 ccttctgtat tgattgcttt tttgattggt gggctgattt tatatatcgt gatgaaaggt
     6061 ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg gattatctgg tttgatacgc
     6121 ccttatctag gtgctcattc tgctgatttt actgattggg tctactggtc tatgtggatt
     6181 atcaatatca tcgctgaagc tgttgcagct gcttcctttt tgcagttgtg gttcccgcac
     6241 attccagcct gggtatttgt ttttatgttg gctgttttaa ctacgatcat caatctttat
     6301 tcggtacggt tattcgcaga aacagaatat tggctggctt ttgctaaaat cagtgtcatt
     6361 atcttactga tcatttttgc tacttatctt gtcggtcagc aaatgttggg cacaggcgtt
     6421 ttccctaccc ttcagcaatt gactgaccat ggtgggttta ctcctcatgg tatgaaggga
     6481 atcatcagtt ctttgcttgt agttatctat tcttacggcg gttctgaact gattgctatc
     6541 acggtaagcg aagcagatga tcctaaacgt gcgattccta aagcaatcaa aggtgtcatg
     6601 ggaagaatca tttcctttta catcattcct ttgtttttgc ttttgattct ttttccttgg
     6661 aatactttag caggcacgaa tgtcagtcct tttgtgatgg tatttgaaaa aatgaatatc
     6721 ccatttgcag cggatatcgt caattttgtg attgtccttg ctttgttctc atctatcaat
     6781 tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt tgaaggatcg aaaaggttcc
     6841 tctagaaaac tagctgtttt aaacaagcat caagtaccgc aacgtgctgt tttattttgt
     6901 accagtgttc tttatcttgg tgtagctctt tcttattttg tcggtgacaa attatttggc
     6961 tatctggcag gttccctttc gtatacagta ttattgatct ggatcattat cagtgcggca
     7021 tcctttgtac tggcactaag aaaaggaaca ttatgggata aaggaatcag cttttttgca
     7081 ttagctgtct taggattgat tttccttggt atcctgttca cgaactccat tggggtaact
     7141 gtattgactg gcctgcttta cctgttcatt tatttcagtt atcaaaaaaa gaatgatgcg
     7201 ttcgtattga cgaatgaatg aaatcgatag aaaattttat tttatttatg tggaatagat
     7261 ggagctattg aagaaaggag ttgtgacaaa agtcttgttg caactccttt ttccgaataa
     7321 acagtctcat gacctcttgg taaacggtgt tcaaaaaccc tctgtgcaaa ataattgatt
     7381 gttctccatg ctcatgacat gatagaagaa aggaatgaaa aaaaggagtt tgttttaaat
     7441 gaaaaagtat tggaagtttt tgttgatgat cggtgtttca acggtagtta tgtttggtat
     7501 gatgtacttg aatgtctatg cgctcgatca tatcttcttc agtcgtacac gagtattcat
     7561 ggcgttgatg atgggtgcag tcatggcagt catcatgctg ttgttcatgt ggaaaatgta
     7621 tgaaaacaaa aagataaatg ttatgatctt agccgctagt cttcttgttt ttgccggttc
     7681 cttgtttatg gtacgaagcc aaactactgt caacgacaca gcttggatga aggccatgat
     7741 tcctcatcat tctatcgcta tcctggcaag taaacgagct gatttaaaag atcctgaagt
     7801 aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa gagatcgaca aaatgaaaaa
     7861 aatgatcaaa gaaatggaat gaccattatc cgtccaaata aaaaaagagg atgttccaga
     7921 agttatcagc ttcaagaaat aagaagaagc tacttctgga acgccgtgaa taaggggttg
     7981 tgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagc ttctcctatt
     8041 tttcgtttgg ttaggcttat taccgcagat gcaagctttt gaacaccgtt tattaagagg
     8101 ttgtaaaatc agcgttctga cctgagtatt agagagacaa atggaaaaat agttcctcat
     8161 attttgccat tttttgagct aataccgagg gtcagctgat tgaacaccgt ttatcgagag
     8221 gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat tttgtgaaat ggctttccac
     8281 atttttgtat aaatttggct taataccgag aaactggctt ttgaataccg tttattcgta
     8341 aaagggattg tgacaaaagt cttgtcacaa tcccttttta tgtttttatt gttctaattg
     8401 cgctgtcttg cttactgctt cttgcggttt cacaaatttt tttgagattc cccaacctgt
     8461 gaaggcactg accatcgaaa acacaggaga aagcaaactg aaaaatacga atggcaagta
     8521 agtgagtgta ggaataccaa atgtacttgc tacgaaactt ccggcaattc cccaaggcac
     8581 taagtagttg attactgttc caccatcttc tagcgttcta cctaatacag ttggatcaaa
     8641 tcctgctttt tcataagttt ctttaaatgc attaccaggc agaataacag ataggaattg
     8701 ttcaccaata aaaatattta ctccgatact tgtcatcaat gttgtaaaaa tcaaatttgg
     8761 tgtgttcgtc atctttcttg atactcgttc catcacggtt gagatcaagc caaattcgat
     8821 caacaatcca cctaatgaca aagttagaat aatcaatgac acagtaggca tcatactttc
     8881 gattcctccg cggctaagca gtgaatcgat ttgttggttt ccagtatttg aaacaaaccc
     8941 tgattcgatg accccagcca acttcgttaa ggaagtttgt gggttttgaa taaaaatcat
     9001 tcctactgct acagcgatat tcaacaaaat cgttaaaatt gccggcacct ttttccatgc
     9061 acacagtaac attaaaaata aaggaatcag tgaccagatt gaaatagtaa agtttgcttc
     9121 tagaacatca gtcacttcac gaatagatga aagacttgcg gctttacttg tgtgtcctaa
     9181 aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca gttgaccaca tcatatgctt
     9241 gatgtgtttg aacaaatcgg catcaacaat agctgctgct agatttgttg attcggataa
     9301 tggagacatc ttatctccaa aaacggcacc tgaaacaata gctccaacaa ccagcgctgg
     9361 atttaagcct agtgttgtcc caataccgaa aaatgcaatc ccaatcgttg acataacagt
     9421 aaaggcactc cctacagcgc tacctacgat tgcacacaca acaaatacag aagggacaaa
     9481 ccactggaca ctgatcaatt tgaacccaat gaccattaga gttggaatga tccctgcttg
     9541 tatccataca gcaatcaatg aaccgactaa aataaaaata aagattggga tgattcctgg
     9601 tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc gggtatcttt ttatcactgc
     9661 atacaacatg accattccga tcgctgctaa gatcgtgata ttaggtgaga cctttaatcc
     9721 gacaacgcca atcgcaatgc ctaaaacaat aaccattaat acaagtgttg cttctaaaaa
     9781 acttggtgtt cctttttttc ccatgataat ctctcctttt ttagttgttc ttatgttcta
     9841 aaaaagttaa gatatcggat tactccgccg caattcatcc gcgattcctc ctttataaaa
     9901 ataaaaagac ctgttttaaa ctaaggacga atgactccgc ggtaccacct taattaaaaa
     9961 cagatctgtt tttcactttc aaacttttat tcaaatgtat ggactttcca tgtaattcgt
    10021 caattaggct tgatcggttc acagcgacca ccgactttct gaacaaaaca cctaaccgct
    10081 actgcgtgaa aagttgactt gtacgaagtt taccttccat aagatcattt gtcaatgaaa
    10141 aagaaaatat tttttcattt ttctgaaaat tatttttata actttcacac acaagtttat
    10201 tttttactag aaagtagcgc cctcttgttt ttttcttgct actatttctt gatgttcttg
    10261 cttgattctt tctaatagct ttggctcttt caacagatca agagctgtat tggcgagtag
    10321 ttctgctgca atcgcaatag aagcaaggcc tttgtcactt ttagcagcag ctttgaattc
    10381 ttctgtgtgt ccggcaatat atgtctcaga gattgctacc gtaggctgga ttgttggtat
    10441 gacttggctg acatttccta catcagaaga accgagactt gttctttgtt ccgtttctat
    10501 ttcttcatct ggaacacctg cttcttttgc atgctggaag aataagtcat caaaagaagg
    10561 agtaacgatg acatcatcaa ctgcattttg gaacaatcca aactcaaaag tcgtgcccgt
    10621 acttatcgca gcacctcgta cgatattttc cactttttca tatactttat ctaatgtttg
    10681 ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa tctggaatga cgttggcagc
    10741 tactcctccg tctgtaatga tcccatggat attgacatct tttggcaatt gcaagcgtag
    10801 agcgttgatt ccattaaaca cttggatcaa tgcttccaaa gcatttactc ctttttcagg
    10861 tgctgctgct gcatgggaag cgacaccgta aaatttgatg tcgactggat cattcgctaa
    10921 agattctgtc gttttcccat aacgaaaagc tggatggacg cataacgcta catcgacatc
    10981 ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat ccgttctcgc ccccttcttc
    11041 ccctggggtg ccatatacac ggatttctcc accagtttca tcaatgaatt gcttcactat
    11101 gctggcggct aatacggagt aagtaccaaa caaattatgc ccgcatgcat gaccaatacc
    11161 tggtaaagca tcgaattctg ccaaaaaggc aattgtggga ccgggtttat tggaaatata
    11221 acgtgcatca aaggctgttc ggtgtccagc tacttctttt tctacccaaa acctttcttt
    11281 ttctaattgt ttaatcaacg ctgctgagga ataaaactca tagttactta cttcaggatg
    11341 ttcgtggata tctagagcaa gtgcttgata ttctggtaaa cgatattgga ttagatcctt
    11401 caattggttt gttgagattg ttgttgtcat aaaaaattcc tcctatgcta gtcatttttc
    11461 ttattttctc ccgaatgttt cccatgcagg cacgcttgat ccttttgatg ttttctcgat
    11521 tactttgact gtgtcttctg tttgataagc gtctactatt ttttgatacg ttttattgtt
    11581 ttcatcttct tttcttgcaa cgatgatatt cacataagga cgtgcatggt catccactgg
    11641 ttctaagaag atggcatcag aagcaggatt aaatccagca tctacagcta caccgctatt
    11701 gatcactgaa atgtctacat ctgacaaagc acgtgctgtt tgggaagcat ctaattcaga
    11761 aatgtctaga ttttttctat tctcggtaat atcagaaaca gtgggcgctt gtccttttgc
    11821 cttatccact ttgatcaatc cagcagtttg caataacaga agagctctgc cgccatttgt
    11881 aacatcatta gggattgcta cttttgctcc gtctttgata tcagctacat ccttcaaagt
    11941 gttagaataa attcccagcg gtgcattgac agtatttccg atcgatacta aatcagttcc
    12001 gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg aaagaattca aatcaatcga
    12061 tccatctgct aaagccacat ttggttgatt gtaatctgta aatttcacgt attccaaatt
    12121 aatgttctcc tttttcaaac ggtctttcac gttgtcccat acatctgtat cttctccaat
    12181 aatccctagt tttacgatag tttctttttt gtcagttgta tctgcttttc cttggctaca
    12241 ccctgccaac caaataattg ctacaaatgt aatgactaca aaacgaataa tttttttcat
    12301 tttttctcct ccactttcaa tgtgtaattt ttttaatcag ttgattactg ataaattgac
    12361 ttaaaaaaac taagattaga atcaataatg tagccacaat cgttacatct gtttggaatc
    12421 gattataccc tctcgatata gcaagattcc ctaatccgcc tgcacctact gctccagcca
    12481 tggtagtcag tccgatcacg ttgatgatag tcaacgccga gacccgaatg ataccaggca
    12541 aaccttctaa caaatacaca cgtaaaatga taccgattgg actggttccc atcgcttctg
    12601 ccgcttcgat caccccagga tcaacttcta gcagtgcgtt ctcgatttga cgagcaaaaa
    12661 acggaacaac tccaaaaatc aatggcacta aagctgcttt cgcaccaatc gtcgttccaa
    12721 ctaagaaacg agtagtcaat gctaaaagag aaagcaagat aatgaaaggg atggaacgaa
    12781 tgatattgat cagcttatca agtatttcaa atagcagacg gttagctagt aatccattag
    12841 ggcgtgtcac tactaacgtc acacctaaga tgattccaat catcccgcca atgaatgctg
    12901 tccaaaaaga catatatagt gtttgcttcg tcgcttcgac aaattcatcc ggaatcaaat
    12961 aaacattggg caaatatgtt ttaaagaaat cagtcatttt atcttcctcc ttcgatcact
    13021 ttgatacgac tatctaaatc gtttttttgt aaaatattta tttttacacc agatttgatc
    13081 aaataatcca atgctttttt ccgctgtttg gtttctccag ctaaagtaac gatcaaactc
    13141 cctaacggcg tttcttgtaa taactctaca ttgccatata aaatattcgc tgttacttga
    13201 tatttgctgt acagatgagc gattagaggt tcgttcgtct gatttccaat ataacttaat
    13261 tccaccagcc atttattggc gatctgatcg gcaaaagcag aatgggcaat gatcgtttct
    13321 aatgcttggt caagatgagt agctgttcga ataaaatctt tggtcaattc ttcttcagga
    13381 ttgctgaaga tctggatgct gctacctttt tcaacgattc ttccatcttc cataaccgct
    13441 accttgttgc agatttcttt gacaacttgc atctcatgag tgatcaggac gattgttaaa
    13501 cctaactggc gattcaattt ttttaacaat gcaaggatct gaagggtcgt tttcggatca
    13561 agtgcactgg ttgcttcatc acataataat accttaggtt cattcgccaa agctcgtgcg
    13621 attgctactc gttgtttttg tccccccgac agttgtttag gataagcaga tgctttttct
    13681 tccaaaccga caagttccag tagttcattc actttttgac gacgttcctg tttcgatttt
    13741 ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag ttcggctttc cattagattg
    13801 aaatgctgaa agatcatacc gatggccttt ctttcttttc gcaattcttt gggagagagc
    13861 tgactcagat tcgtctgatt gacaatgact tctcctgctg ttggtttctg cagcaaattc
    13921 atcacacgaa ctaacgtact tttccctgca cctgagtaac cgacgattcc ataaacatcg
    13981 cctttttcaa tcgttaaatc tacagaatct actgcctgta tctctttttc tttttgtttg
    14041 aaacatacag aaacatctcg caattcaatc attaaaattc ctccttatct aaaaaaagcg
    14101 tcctcaagaa aatgaaaatg tcattttctt gaggacgctt ttttgcgtgt taccacctca
    14161 tgttcaagct gtgttcacac acaactcctc ttcaagtacg atcatactct agcaagatat
    14221 cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg aattgctcga tggccatttt
    14281 ccaatagtcg ttccttgctt cttctcagct accgaagctc tctgtcaaag gttcttctat
    14341 cgtacttacc atttcaatgc aaataatatt caaaaaaaaa gtccttttac taacataaac
    14401 tatgtcagta aaaggacgac gtatcgcggt accacctttt ttcggaagtt tccttccctc
    14461 aagcccaaca tctcaaaaag agagattggc gttcgtttta acggtcgaac agttccgaat
    14521 ttgcctactt atcgacaaat tattctccaa ggccattttc agcttttctt acttgactct
    14581 tctcaccagc cagagcctct ctgcaaagtt tccaaactta ctttcctttt caacgaaatc
    14641 gatattttgt tgttgggatt aatctacatg aggaaaactg aaaagtcaag tacgaaattc
    14701 aaattatttt ttctatattt atttttctgt ttttttattt aacatttcaa taacagttcg
    14761 aagatctttt ccttccgcaa gaagtttcaa tttttccttt tcttcgacgg tataatttcg
    14821 gacaaaactg gaatcaggta tcaaaacaac gcctaatcct gctcgtgaag cagctgttgc
    14881 tcctactaaa gaatcttcca ctgcaaaggc tgaactagct tctaatcctg ttcgttccaa
    14941 agctgtcaga taaggaatcg gtgaaggctt gttctccgac acttcgtctc cgaatgtaaa
    15001 cgtgtccaag taatcagcta atttaaagtg atccaagata tctgttgctc ttttcgtaac
    15061 agtggtcgta gcaatgccta gtttcatatg acgttctttt aagtctgcca atatttcttt
    15121 tgcatatggt tttaagtgga tggctccgct tgtcagctgt tgataaaaat attcttcacg
    15181 cttcgctcgg acttcctgta ctttttcagg gctacctgct gctttcacta aaatatcaaa
    15241 actttgtttc cagttttttc cgccccaagc gtcaacgatt tcttttggga tatggatatc
    15301 aaattgctga aatccccaaa gccagccatc acgatacgtt ttttctgtgt caactaacaa
    15361 accatctaaa tcaaatattg cacctttcat ctatacatta atcctttcca aaacaataca
    15421 catagtttag cacgactggt ccaaaaagaa cagaagcaaa gaaccagcct tctcgtttat
    15481 aagagttctt ggaatcgacg gatatagatt ttcttcacag ctgtcacaag cgccagataa
    15541 gcgatgactg tagcaaataa ccataaccag taattgccag gaagtggcat caagcctaat
    15601 tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa taattcctaa tgtagtgata
    15661 acaaacatgg caaatgttgc gttgctttgg ataaatggta ctttcggtgt acgcagtgca
    15721 tggagcacta aggtttgtgt ccatagtgat tcgacaaacc atcctgcatg gaataatgcg
    15781 atgaaaatca ctcgctgtga actatccaat gtatggaaac ttccgcccac tacagcagga
    15841 caaataacaa aatacatcaa caagtaagta gtaatatcaa atatcgaact tgttggacca
    15901 aagtaaacca tgaattcccc aatactagat gcattccatt tttttggctc gtccaaatat
    15961 tctttatcca tccgatccca tggcaaggaa atgcaggaaa catcatagat cagattcaaa
    16021 aacaaaagtt gtaaaggcag cattggtaaa aatggcagga acgtacttgc aatcagcacg
    16081 gaaaacatat tgccaaaatt agaacttgct gtagccttga tatatttcat gatattccca
    16141 aaagtttctc gtccagaaag aaggcctctt tccaaaatcg tcaaatcttt ttctaaaagg
    16201 atcacgtctg cagattcttt cgcaatatcc acagctgtat ctacagaaat accgatatca
    16261 gccactttca ttgcaggagc gtcattgata ccatctccta aaaatccgac agtatgacca
    16321 tttttacgta aagctgtcgt caaacgtgct ttttgctgag gattcaattt tacgaatact
    16381 tcatggtctt cagctacttg tgctaattca tgatcactca tttccgagat tttctcaccg
    16441 gtaaccaatt cattgatctc tagacctact tgacggcaca ctgaacgtgt aactaattcg
    16501 ttgtccccag tcagaacttt tacagctaca ccatgttctt tcagagcttt caacgcttgt
    16561 ttcgttgttt cttttggtgg atcaaggaaa gcaagataac caatcaaaac catgtctgac
    16621 tcatctttga ctgaaaattc tccaacgacg cttggattgg tcttttgtgc gacaccaatc
    16681 actcgtaagc catcttcatt aagtgactgt acttttgcaa gtacggcttc ttttcgttct
    16741 tttgttaatg gtgtaacagt accgccaaga tcaataaatt tagaaatgtt cagcatttct
    16801 tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg gatcagcaac cacaacactc
    16861 atacgacgac gttcaaaatc aaaaggaatt tcatccactt tttgataatt gatcttatct
    16921 gtatctaaag tttctgttgc ttcatcaatg atagccacat ctaacaaatt tttcagccct
    16981 gtttgatagt agctatttag atacgcatgg cgcaaaacac gatcatcttc atttccttca
    17041 caatccatat gatattcaag aatgatttta tcttgtgtca atgtccccgt tttatccgta
    17101 cataacacat cgattgcacc aaagttctgg atggcattca aatttttgat cactgttcct
    17161 tttttggcca tcacacttgc tcctttgacc aaattcgtcg taacgatcat tggaagcatt
    17221 tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa aagcttctag ccaatcccct
    17281 tttgtaagcc cattgatgac gataactgct ggagccatca atgacataaa tttgatcagt
    17341 agcatggatg ttttatggat gcctaattca aagcttgatt gaattggttt ttcagatagc
    17401 gtttctgcta catgaccaaa tagtgtttca tttccaacag aaacaatgat tccttccgca
    17461 cttccactga tgacattact tcccataaag acaagatttt cgtaacttgt ttcgctttgc
    17521 tgatcgtctt cacatacttt cgcatgcttt tctactggat agctttcccc tgtcaatgct
    17581 gcttgggaaa tgaataaatc ctttgcggac aataaacgca tatccgctgg tatcatatcc
    17641 cctgctgaaa gccgaacaag atctccacag acgatttctt ccataggaat ctctgtgtac
    17701 tttccattac gttttacagc tgccgttact ttcacaagtg atttcagttt ttcagccgct
    17761 tgattggaac gtacggactg aatcaacgtc attgtcccgc tgacgataac catagtgaag
    17821 ataatggcta caccgaaaag atctttatct ccaggagctg ctaatacata ttccgtgatg
    17881 aatgaaataa ttcctaatgc gatcaacact aatgtaaatg gcgtgatgta tgctttcaat
    17941 atttctacaa tgaatggtgt ttttttgcca tatgaaatcg cattttcacc gtaattttct
    18001 cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg atgcatggaa tagttcaaat
    18061 aattcttgtt tcgtttttcc agcaaaagct gcatagattt tttgtgtatc tttctttttt
    18121 tccatctcaa tctctccttt gtgtccaaaa gacagctgtt ctcgctgctt ttggtcttta
    18181 gctattcatc ggatcgatag agaaaaaact gaaaggggct gagaacagat agataatagc
    18241 cctggccttt cctctagatc cttgtttttc gaatgactgt catccatttg ttctcatctc
    18301 ctttactaag taattttaat tcatgacttc caatcatttt tcgaatagtt atttttccaa
    18361 ttagaccacc tcctttttat ttttcaccga tggggatttg cggcaacaaa aaaacctctc
    18421 gccaaatact cagcgaaagg tttgaaaaag catattaaaa aaagcttctc agatctcatc
    18481 gttgagtttt gacactgtac aacgtaaaaa gcagatacct gcttttagct accttatgaa
    18541 aagccttatc tcgacaattc ctgttctacc cattggcgtc tctcgacgtt tttgggcagt
    18601 agcctatgtt tgtataggag cctcacctaa caaatcttat tcggttttcc ttcgttaata
    18661 taacaactaa aaaagaggat gtcaaatcaa ttttccatgt ttttcatact ttttttaaaa
    18721 tacacacgtt atattcctgt ctcacgcggt tttatttgtc tcattttcca cctcccgcta
    18781 taatagaaat atagtaggaa acgaggtgct gttatggaaa gaacacttgt tattatcaag
    18841 ccagacggcg tgagacgcca tttagtcgga agcatcatcc aacgttttga agcaaaggga
    18901 ctggccattg ctgaaatgaa gtttgaaact atgactcctg aattagcaaa agaacattat
    18961 cagcacttga ctgagcgcag tttttttgat gaactgatcg attacatgac ttccggtcct
    19021 gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata tcgtccgtaa gatggttgga
    19081 gcaacaaaag ctgccgatgc tgttccaggt acgatccgag gagactacgc gctgccgggg
    19141 actgaaaaca tcattcacgc ttctgattcc cgtgatgctg cggtaaaaga gatcgcccgc
    19201 tttttcccgc cattcgatat aaacaaaccg tctgaaaaag cggcgaagtg atacaaattc
    19261 taaactgctg gtctagttgc tgggttcagc aatggccagc agttttttct ctaaggacct
    19321 ccgttgattc tttcctaaca tatattcgag cactgtgaag aattcttggt agtctttcgt
    19381 cttttcttcc cctaaagcag cagaaatcaa tctttgataa taatctgcca tcaatgtttc
    19441 atagatcact gtacttcttt ctttatgtgc taagctccgt ttcatatctt cattcacact
    19501 acgtgccttt ttcaaccgac cattttctat ataaaacgaa gtcaggttca ttaaaaaagt
    19561 aaagagatac tgctcatgat tgttcaactg tgctaatcgt cgcattcttt ttttgatttt
    19621 caaataattg ttctcaatga accgatcact gaatatccaa ggaaaatcag ctagaataga
    19681 tagttcaaat tgaccatagt ctggtatctg catcaagtat tcttcaatga ttcggcattc
    19741 ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt ttttcgacca tgtgaagatt
    19801 caaaaccaaa taatacagat cttctgtttt cttaaaatgc ctacggttat tgctgatctc
    19861 tttataagga tcaaagctgt cttcttgtac ccgatctagc attcgtcttt ttttctttaa
    19921 ctttattggt tcatccagct ggtttcgcat ccattcatat tctgcaagtg tcatgttcat
    19981 tcgctctaaa aatgatctca atgttacaaa tgacattttt ttcactgaat ttgtctcaat
    20041 cccgactaga gaagtcttaa aacagattcc ttccgctaat tgggactgtg tcaacttctt
    20101 gttgactcga agtttcttga tcaattcacc atcatacata atagttctcc tttttaattt
    20161 aaatttttcc acattcaata aatagatacg ttaaaaagga aaaatacacg aaaaaaataa
    20221 aaaaatgttt ttttcataaa aaataccaac cttcaagcat tagaatgttg tctaactact
    20281 gaaggttggt ttgattgttt attgattatt t
//
LOCUS       DACDMM010000040        19520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDMM010000040 DACDMM010000000
VERSION     DACDMM010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.040"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(24..203)
                     /locus_tag="IWD16_001094"
                     /pseudo
     CDS             complement(24..203)
                     /locus_tag="IWD16_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(407..766)
                     /locus_tag="IWD16_001095"
     CDS             complement(407..766)
                     /locus_tag="IWD16_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP9088174.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="IWD16_001096"
     CDS             complement(796..1137)
                     /locus_tag="IWD16_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088175.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="IWD16_001097"
     CDS             complement(1134..1808)
                     /locus_tag="IWD16_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9088176.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="IWD16_001098"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="IWD16_001098"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP9088177.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="IWD16_001099"
     CDS             complement(4322..5512)
                     /locus_tag="IWD16_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9088178.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="IWD16_001100"
     CDS             complement(5533..6060)
                     /locus_tag="IWD16_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9088179.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="IWD16_001101"
     CDS             complement(6107..8896)
                     /locus_tag="IWD16_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9088180.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="IWD16_001102"
     CDS             complement(9043..9243)
                     /locus_tag="IWD16_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9088181.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="IWD16_001103"
     CDS             complement(9696..10016)
                     /locus_tag="IWD16_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088182.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="IWD16_001104"
     CDS             complement(10519..11637)
                     /locus_tag="IWD16_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088183.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="IWD16_001105"
     CDS             12108..13415
                     /locus_tag="IWD16_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9088184.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="IWD16_001106"
     CDS             13536..14735
                     /locus_tag="IWD16_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9088185.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="IWD16_001107"
     CDS             complement(14762..16030)
                     /locus_tag="IWD16_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP9088186.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="IWD16_001108"
     CDS             complement(16200..16841)
                     /locus_tag="IWD16_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP9088187.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="IWD16_001109"
     CDS             complement(17250..17753)
                     /locus_tag="IWD16_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9088188.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="IWD16_001110"
     CDS             complement(17876..18640)
                     /locus_tag="IWD16_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP9088189.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="IWD16_001111"
     CDS             18748..19023
                     /locus_tag="IWD16_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP9088190.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
BASE COUNT         5598 a         4146 c         3464 g         6312 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgtcatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga
//
LOCUS       DACDMM010000041        19200 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDMM010000041 DACDMM010000000
VERSION     DACDMM010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.041"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            100..2541
                     /locus_tag="IWD16_001112"
     CDS             100..2541
                     /locus_tag="IWD16_001112"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP9088191.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2871..3575)
                     /locus_tag="IWD16_001113"
     CDS             complement(2871..3575)
                     /locus_tag="IWD16_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088192.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3762..4007)
                     /locus_tag="IWD16_001114"
     CDS             complement(3762..4007)
                     /locus_tag="IWD16_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088193.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4024..4875)
                     /locus_tag="IWD16_001115"
     CDS             complement(4024..4875)
                     /locus_tag="IWD16_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9088194.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5041..6045
                     /locus_tag="IWD16_001116"
     CDS             5041..6045
                     /locus_tag="IWD16_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP9088195.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6286..6642)
                     /locus_tag="IWD16_001117"
     CDS             complement(6286..6642)
                     /locus_tag="IWD16_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9088196.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7010..8197
                     /gene="tuf"
                     /locus_tag="IWD16_001118"
     CDS             7010..8197
                     /gene="tuf"
                     /locus_tag="IWD16_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9088197.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8385..10976)
                     /gene="mprF"
                     /locus_tag="IWD16_001119"
     CDS             complement(8385..10976)
                     /gene="mprF"
                     /locus_tag="IWD16_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9088198.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..11721)
                     /locus_tag="IWD16_001120"
     CDS             complement(11002..11721)
                     /locus_tag="IWD16_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9088199.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12041..12562
                     /locus_tag="IWD16_001121"
     CDS             12041..12562
                     /locus_tag="IWD16_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP9088200.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12621..13481
                     /locus_tag="IWD16_001122"
     CDS             12621..13481
                     /locus_tag="IWD16_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP9088201.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13588..15981
                     /locus_tag="IWD16_001123"
     CDS             13588..15981
                     /locus_tag="IWD16_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9088202.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16163..18004
                     /locus_tag="IWD16_001124"
     CDS             16163..18004
                     /locus_tag="IWD16_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9088203.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18028..19137
                     /gene="rpoD"
                     /locus_tag="IWD16_001125"
     CDS             18028..19137
                     /gene="rpoD"
                     /locus_tag="IWD16_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP9088204.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
BASE COUNT         6228 a         3392 c         4028 g         5552 t
ORIGIN      
        1 taaggattgt taaaatttaa tcttagaaaa aaataactgg aacaagagct atttgttttt
       61 acagcaaaga atggtatatt tttgtaggag gtggcttact tgaaagaagt cgttatgata
      121 gatgctgcaa gaacgccgat aggaaagtat cgcggaagtt taagtccatt tactgcagtt
      181 gaattaggca cacttgtgac taaaggatta ttagataaaa caaaactgaa aaaagataaa
      241 atcgatcaag taatatttgg aaatgtcctg caagctggta atggtcaaaa tgttgccaga
      301 cagattgctt tgaacagcgg tctccctgta gatgtcccag caatgacgat caatgaagtt
      361 tgcggatctg gtatgaaagc agtgatctta gcacgccagc tgatccaatt aggtgaagca
      421 gaacttgtga tagccggcgg aacagagagt atgtctcaag cacctatgct gaaacagtat
      481 cagtcagaaa caaatgaata tggtgaacca atttccagta tggtcaacga cggattgact
      541 gacgcatttt caaatgcaca tatgggatta accgcagaga atgttgcaac acaattttct
      601 gtgagcagag aagagcagga tcgctatgcc ttgtcgtccc agttgaaagc agcacatgct
      661 gtcgaagccg gtgtattttc tgaggagatc atcccagtca agatttctga tgaagacgtg
      721 ttatctgagg atgaagcagt tcgtggaaat agtacattgg aaaaactggg cacgttacgt
      781 acagtattct cagaagaagg aactgtaaca gcaggaaatg cttccccgtt gaatgacggt
      841 gcctctgtgg tgatccttgc atccaaagaa tacgcagaaa ataataatct gccttattta
      901 gcaaccatca aagaagtagc ggaggtcggt attgacccaa gtatcatggg aatcgctcca
      961 atcaaagcca ttcaaaaatt gacagataga tcaggaatga atttatcaac aatcgatctt
     1021 tttgagatca atgaagcatt tgcagcttct tctatcgtag ttagccaaga actgcagtta
     1081 gatgaagaaa aggtcaatat atatggagga gcaatcgcat taggccatcc aattggtgca
     1141 agcggtgcac gtatcttgac tacattagct tacggattgc ttcgtgaaca gaagagatac
     1201 ggtattgctt cattatgtat tggtggaggt ttaggcttgg cagtcttgtt agaagcgaat
     1261 atggagcaaa cacataagga tgtgcaaaaa aaaaagtttt atcagcttac gccatctgaa
     1321 cggcgcagtc aattaataga aaaaaatgta ttgacacaag aaactgcgtt gatttttcag
     1381 gaacaaacac tttctgaaga gctctccgat cacatgatcg aaaaccaagt gagtgaagtg
     1441 gaaatcccaa tggggatcgc gcagaatttt caaatcaatg gcaagaaaaa atggattccg
     1501 atggcaacag aggaaccatc tgtcattgca gctgcaagta atggtgcaaa aatctgtgga
     1561 aatatctgcg cagaaacacc tcagcggctg atgcgagggc aaatcgttct atcaggaaaa
     1621 tcggagtatc aagcagtgat caatgcagtt aatcatcgaa aagaagagct gattctttgt
     1681 gcaaatgaaa gttatccttc aatcgtgaaa cgcgggggcg gtgttcaaga tatctctact
     1741 cgggaattca tggggagttt ccatgcatac ttatctattg attttttagt ggatgtaaaa
     1801 gatgcaatgg gcgctaacat gatcaactcg attcttgaga gtgtggcaaa caagttacgg
     1861 gagtggttcc ctgaagaaga gattctattc agtattttaa gtaattttgc tacagaatca
     1921 ttggcttctg cctgttgtga aatcccattc gaacgcttag ggagaaacaa agaaatcggt
     1981 gaacaaattg ccaaaaaaat ccaacaggca ggggaatatg cgaagcttga cccttaccgt
     2041 gcagcaacgc acaacaaagg gatcatgaat ggtatagaag ctgtagtggc agctacagga
     2101 aatgatacac gtgcggttag cgcttcgatc cacgcctacg cggcaagaaa tggattatac
     2161 caaggattaa cagactggca gatcaaaggg gataaactag ttggaaaact aacggtgcct
     2221 ttggctgttg ccacagtagg cggagcttcc aatatcctgc caaaagcaaa agcatcttta
     2281 gctatgttag atattgattc agccaaggaa ctagcacaag taattgctgc agtagggtta
     2341 gctcaaaacc tggctgcttt gcgtgcatta gttacagaag gaatccaaaa aggccacatg
     2401 ggacttcaag ctcgttcatt agcgatctca attggggcta ttggtgagga aatcgagcaa
     2461 gttgcgaaaa aattacgtga agcagaaaag atgaaccagc agacagctat acaaatattg
     2521 gaaaaaatcc gggaaaaata aaaaagcaat atctttagaa gaataaagca agatttacag
     2581 ttattttatt tactgcagat cttgcttttt tctctatcag aaaatatgtt tattattaga
     2641 attttttata gttaaggtta ggcatcttaa gaaatcaggc aactccaaga tatcttcagg
     2701 tagaagagaa gtcgcctaac attgtaggat cataggtgat ttgttggaca ggtcaagctg
     2761 atatttgagc ctgatgttag aaggaatttc tttttacttg ctgtagaaat tattatatga
     2821 ctaaaaaggc tgggatttta aatcccagcc ttttatgaca aatagaattt ttatcgatca
     2881 gtaatcgctg tcgctgcaaa ttgttcgatc aaaggaatca aagtcaattt gactttgtat
     2941 ttccgttcga gtgctttttc gataacgaac tcattttcag tagtcataga aatagttgct
     3001 aatttacgag gttcatcagc atttttttca ttaataacta cttcatgttc attttgttct
     3061 gtgtaaaatt taggatactc agcaacggct tttgaaaaat cagcaaaact taaagcggca
     3121 ttcgtatcag ttgagcgtgt attttcagtt gtgtccgctt ttggtttttc cgttttttct
     3181 tctggcacac tgactgtttc ttctgtaact ggtgtcgcaa cagcttgcgc agttggttca
     3241 tcgtttttca atcgttgatc attccagtaa gacacgattt caataagaag attgtcacgg
     3301 aatcgttcga aaatcccgtt ttctcctgaa cgaattctct ttaatacagt attgaagttg
     3361 tcatcgattg gcacgttgat ccgtgtatct tcatttgttt tcccagctaa tccgatcgtt
     3421 aagatttgac ggtttttggg atcaagcatg acttgtaacg gcaaaccata acgtcgcaag
     3481 ctctcttctt ctagagaacg gaataattgt cctcttacac catccaatga aggacggaat
     3541 tgatttaata atttttgttt ctgttttttc ttcatatgtt ctctccttcg ctcataccct
     3601 ttcgattata agaaaaaaac agccaaatga aaaggagtaa tgcaaaaaaa cttgcgtata
     3661 cgcaaattac aacagaaaaa tttgcgtatt ttcccgtttt ttgaaagaaa aataaaaaat
     3721 ggaacggatt atgtatatat acataatccg ttccattctt tctatttttt cggtatcaag
     3781 gcaaaaacag gtagttcagg tgaataaatg acttcatgtt cgtgccaggg tttgaccatt
     3841 agcgaatgat cgaaccgttt tttcagttct tcgatgattt gatcatgtgt ccagtttctt
     3901 gcaggaaaat gttggatttt ctctgcatcg atcaaaaaga caaaatcgga atgaaaagtc
     3961 tgggtaactt cttccgagag agaattcaag cgttcgattg gatccataaa atcacctttc
     4021 gacttaaaat tgggtagtat ctgggtcttt tgctttgccg actacaccat ctaactgatc
     4081 gatttgtttc atttcttctt ctgttaattc aaaatcaaat aattcagtat tttctttgat
     4141 tctgcttggt gtcactgact taggaagagg aaggaattcg tgttgcagcg accaacgcaa
     4201 agcaacttgt gcaatcgtct tgtcatgtgc ttcagcgatt tgtttcattt caggcacatc
     4261 gaagatttta ccagtgccta atggactata tgcttcgagg atcatatcat gtttttttgc
     4321 atactcaact acagttgtct gtaattcgcc tggtgcaagg aaaacttgat tgaccatcgg
     4381 cataattttt gctgtttttg ctagtgtatc tagatggtgg ggtaagaaat tgctcacacc
     4441 gattgctttg attttacctg cttcatacag ttcttcaaat gctcgccatg tatctgcatt
     4501 tgcttgttcc caattatcac gaaaagcaac agggtttggc cagtggatca aaaacagatc
     4561 taaataatct gtttggagct tcttcaatga ttcttcaaaa gaagacatca caagttcata
     4621 gctgtggttt gcattccata attttgttgt caagaatatc tcttctctag gaattccact
     4681 ttccttaatc gcttggccga cgctttcttc attcttgtat ccttgggcag tatcgatatg
     4741 gcgataacct gcagccagcg cttctttaac agaggaaaca gcgacatccc catcaggtgt
     4801 ttgccatgtg ccgaatccta ctaccgggat ttcgtaacca tttgatagac gataagtgct
     4861 tgttaatgaa ttcatacttt cagctccttt tttattcgtt caactataag tgtagcgcat
     4921 ttttcgaaaa aaagaatcca ttttgtttct actcttgaaa tttttgttta aaagaaaatg
     4981 attttgattc tggtattttt gatttttttt ccgtaaacta agacagaagg gaatgagaaa
     5041 atggatatct atttaaaaaa agcaattctg catattattg acagagaaac tggttctcca
     5101 gtcttttcac aaaaagagct ggatctgaca aaagaatata ttcgtgattt tcttcaaaag
     5161 aagatccaaa aaatctcttc tgctcaaaca aaaactggac aactagcaga agaaagcatg
     5221 atcgttgaat cacttaaaat cgctcaagaa aattttgtgg aagcaagtga agcaatcgtt
     5281 cagcggtggt tcgatatcta ccaagaaagt gaagaagcac ctagtgcaga tgtatttgtt
     5341 gtcttgtatg aattagatac agtgatgtac ttatctttat tgaaagtcaa ttatcgtgag
     5401 gcttacactc actttgttga agcagacgaa gcagggatcg acaaccgctt gattttgaat
     5461 cgagcaattc taggggcgaa aacacaaaaa gcagatgaag cattcgctgt gaatttgact
     5521 gattggacct acgaattaat tgaaaaaaaa tatgagtttt ctggcaaaaa agagctttat
     5581 ttttctggaa gagtgatcga aagtgttcct gcaccttctt tagaagaaaa tgtaaaagtg
     5641 atccaaaaag tagccaagca tcttggaaaa aaatttgaga cagaagaatt tgatatcatg
     5701 gctgatttga aagaagctgt ctatgatacg atagaagaaa aaggtcggtt ggatcatgag
     5761 atgattgcgg aaaaagtctt caaggaaaac atcacggcaa aaatggcttt taaagaagaa
     5821 gtacaagagc aaggatttgt cccagaagca cctcctgtaa aagaagtaca agaaatttct
     5881 gagaaaaaat ttggaaaaca aaaattcaag ctatcaaacg ggatcgaact gatcgtacca
     5941 gtagatgtat atcgcaaccc agatttgatt gaatttgtga ataatccgga tggtacgata
     6001 tccgtgatga tcaaaaatgt ggatgaagta ttgaatcgtt tataaaaatg actagaatat
     6061 taggtgttca agtaccagtg atcagttaaa tgaattgtgt caagagaagg atcggaaaag
     6121 acgaatataa caaaaaaaga gcgtgagaca aaagtaaaga acacttttgt ctcacgctct
     6181 aaacacgtat aaacggcgga agcagaagca actccttctt atttcttgga gttaaacgct
     6241 tctgtcccag cctcttttta tctaataaaa ttttaagacc atttactaat tatcgatttt
     6301 ttctgccgca tttttaggca cttcagaagc ctttatttct tcccagctta atacaccttt
     6361 acgaggattg actttgagtt taagatacgc attcatcttt aatggatgtt ctctttcttc
     6421 gcgaagcgtc acctgttttt tttcaccatc tttattataa gccgcttgct tgtaggtata
     6481 agtcgtgaat ttctctccgt tatctgcttg ggtagctgac atttcgccat tcgtcgtgat
     6541 ttttgtataa taagcttcgc cgccgtaaaa ataatgataa gctgcaaaac ttccgccgag
     6601 aaatatgcct aaaacgacaa gtacgccaat taattttttc atgtacttca cctctttcct
     6661 tattattaac gaaatagaag atttcgtcta ttgaaagttg ttggcaacag ctgaatttta
     6721 aacttttcct cattttgttc ttattgcttc aaaagaaagt taaggactat cttgaagttt
     6781 ttctcaaaaa acacattttt ttatttataa aaggtacttt tttcgcgtat aagacagacg
     6841 agtgctctta gttgactttg accaatattg acgtataata agcttagttc gaatcttaca
     6901 aaaagtaagt cggacgtatt ctttttgatt tagtcaaaaa aagactataa taaaaaggtg
     6961 agagctgaaa tttttcagtg atctatatta tttaggaggt ttgtaaatta tggcaaaaga
     7021 acattatgac agaagtaaac cacacgttaa cattggtacg attggacacg ttgaccatgg
     7081 gaaaactact ttaactgctg cgatcactac agtattaggc aaaaaaggcc tagcaaatcc
     7141 tcaagactat gctagtattg atgcagctcc agaagaacgt gaacgtggga ttacgatcaa
     7201 taccgcacac gtagaatatg aaacagaaaa acgccactac gcgcatattg atgcaccagg
     7261 acacgcggac tatgtgaaaa acatgattac aggggcagca caaatggatg gtgcaatctt
     7321 agttgtttct gcaactgacg gtccgatgcc tcaaacacgt gaacacattt tattgtcacg
     7381 ccaagttggt gtaaaatacc tgattgtttt cttgaacaaa gttgatttag tcgatgatga
     7441 agaattgatc gatttggtag aaatggaagt tcgcgagtta ttgagcgaat atggtttccc
     7501 aggcgatgac actcctgtga tcaaaggttc cgcattaaaa gcattgcaag gcgatccaga
     7561 tgctgaagct gctattatgg aattgatgga tacagtagat gaatatatcc caacaccaga
     7621 acgtgataca gataaaccat tactattgcc agtggaagac gtcttctcaa ttacaggtcg
     7681 aggaactgtt gcctcaggtc gtattgatcg tggtgctgtt cgtgtcggtg atgaggtaga
     7741 gatcgtaggg atcaaacctg aaacacaaaa agcagttgta acaggtgtag aaatgttccg
     7801 taaaacgtta gattacgggg aagctgggga taacgtaggc gtgttgttac gggggatcca
     7861 acgtgacgat atcgaacgtg gacaagtact tgctaaacca ggttccatta ctccacatac
     7921 aaaattcaaa gcagaagtgt acgtgttgac aaaagaagaa ggtggacgtc atactccatt
     7981 cttcaacaac tatcgtccac agttctactt ccgcacaact gatgttacag gaacaatcac
     8041 attgccagaa gatacagaaa tggtcatgcc tggtgacaac gtgacgattg atgttgactt
     8101 gatccatcca atcgctgttg aaaacggaac gacattctca attcgtgaag gcggacggac
     8161 agtaggttca ggtatcgtta cagagatcga agcataagaa acgcgactcg aatattttgg
     8221 taatgaaaaa tgtttaaaat aaaagagcgg tccaaacagg atttctgttt ggaccgctct
     8281 ttgttcttca attgtataag aagagattgt agaagcatga actttcgttt tacttcagtc
     8341 tcttttattc atcatgagag tgttcatcta tcggtatgca gtttttaata ctttcttcgt
     8401 atccattttt tcaaaccact tattctacct tgtgtctgct caactggcgc attatcaata
     8461 atcagcaaag caatcataac atatgcaatc caactatctc tagaatatag agtataacgt
     8521 gagacccagt ttgtagcgta cttgctttta tattctctta atccatggaa agaataaaaa
     8581 tgagaaccaa actcgtaaac caagtatgca atcctttcct gtaaaaaact ctttctggaa
     8641 gttccgacat ttgaaagagg ggccatccct aaattgaacc attggatatg ttcttctttc
     8701 atataggcaa acaaatgcaa gaacaagaaa tccatactgc cagaaggtgc ttttttagaa
     8761 tcatatcgca tcagatcaat cgttcctact ttattgttcg tgtaagtagg catgatcgtt
     8821 gcaaaagcga caatttcttc ttgggtattt ttgacaactg caataggcgc tctttgtaga
     8881 taatcttcag agaaaaagcc taaagaaaac cctttttcct ttcgtgaacc caaccaatta
     8941 tcagagatat ttttgaaagt actcatttgc tcagtagaaa atggtggttg taagacatca
     9001 aatgtaaatc cttctcgttc gatccgattc aagacagcac gagtgccctt catttttttc
     9061 cctgaagttg taaaactatt caggtctaca taagcttctt cccccatctt aatgaaatcg
     9121 tagccgaatt catgcaaaat cataacaatc tcttcgcttg tttcgtagaa aactggtaaa
     9181 tagcatagtc gatctgtttc ttcgataaaa gcttcgattg cttctggaaa atcttctttc
     9241 ttaccagaag gatcacccat tacaatacat ttattattga agcaagcgaa ttggagaaga
     9301 acagttggtt caccgtcttt ttcataggca aacatccgct tatcttttaa gaagatcaat
     9361 tgactatcgg agtttcctcc ataagttgta aggatcttca gggccttttc ttcattaaag
     9421 gcttcgccaa tctgtttctt ttctccttga aggaaatgaa caaatagaac gatcatgaaa
     9481 ctaacagcga tgatggccaa caatcccgaa aaccaaatct tttcagaagg aaataagaaa
     9541 aaggaaataa aatggtgacg ccgatgaggg aaatccggca gattgtatac cccaataacg
     9601 atgtaaagca aggacagtgc accaataatg attccatcga tcgtacgcca ctcccatgaa
     9661 tagatcaatt gttcccgaaa taattcgttt ttagaagcaa gaatgatcag aagcagaata
     9721 gacaagaaaa ccaccggtgt gaaactaaaa tcacttaata aaacataaag taaagctaga
     9781 acaatcaaga agattgtagg caaatatgct cgtttcacac gtgctgcgat ccctctgccc
     9841 ataatgatta aaagaaatcc caatagtata ctaggaaatt ggataataaa atgaaatttc
     9901 aatgggttca aactatgaag ccagcgaaac tctgtaaaag cttgcgggat agtggcagaa
     9961 agtactagca tgattccaga aaagtataat aagacaacca cgattttatg cgcaatttct
    10021 gttgctaatt gtttagggat accagaataa cgctgatcaa aagtggaacc gatatttttg
    10081 aagaaaaaga cgattccaat caaaaacgga atgatgtagt agaacaggcg atagagcaag
    10141 atccacacaa cgactatttc tcttggcaca ccaatagcgg acaacccgat aatcatcatt
    10201 acatcgaaac tcccaatttc tcctggaatc atagacacga ttcctatcac tgaagcagca
    10261 atgaataatg gaattgtttg ccacagatca atccggattt ccattaaata accaacagaa
    10321 aggaaactaa acaggacacc ggtccattcc aacaatgaaa ctagcaatag ctgtccttga
    10381 gttttaagtg aggtaccacc aaggaatcct ttctttttga aagtagtaaa taaaaagacg
    10441 acaggaaaat atagaccgcc gccgatcaac catatccaat attgcttcaa aaaaggagca
    10501 actggcgtga agactacaag taaaaaagaa agcagactgt aaatagaaag tccagccatc
    10561 aaaaacaaga aaactttcga taatgcttgg atcacgtttc tgctctcttt cttttgtcca
    10621 tatagttcag agcgaagccc cacactaaca aacccgccga aaccggcaat attgttgatc
    10681 gtattgatca gccagcttgt ctcaaacagg taacgaggat tctgcttttg ttcaagcatc
    10741 cgattcaaaa taatatcata accaaccata ggtaacacag agataagacc aatcaatagc
    10801 attagtccgc ttttccatac aggtatagtg gcaaacgttt ctcctaattg ctggatagat
    10861 agagttttac cgatggacat caattcaccg acaataatga caatgacaga aatcaaaaag
    10921 attgttttga acagtcctag gtggtttttc agccattgta tggtattttt taacaaataa
    10981 atcaccttct attcctaata gttatgtttc agaaagaaat tgaagaactt ctcgattgaa
    11041 gcgttcagga tcttttctag ctaattcatg tccttgttct ttcacaagca caaaggaagc
    11101 ttttggtata gttttagcta tatacaaaga atgttttagc ttgattacat ctttttttcc
    11161 cacgatgatc aaagtggggg agttgatttt ttttaaatca ttttctgtaa gcccgatgtc
    11221 atgcagcaaa agtttgataa ctaaaagatt tttgcgaaga cttgggcgaa ataaagagag
    11281 taaccatacc catgcataat gaatattact gatgactctg gcagaaaaac gaacgccttt
    11341 aacaagcgtg tttccagaat ttaaaatcaa tcgatgaact tttttaggga agctgctggc
    11401 aaataccagt gctagatttg ctccatcact aaatccaaga aaatctgcct gatcgatttt
    11461 ttccagcaac atgatcgtat tcagatcctc ggccattaac tgaaaattca acatagatgc
    11521 ttcgtttgtc gaacgaccat gtcctcggct atctactaga tacacggtat agtagcgttc
    11581 gagtacaggg acctgttctg aaaaaaagcg gccgctgccg tcattgccat gcagtaaaaa
    11641 gagagggaag ccttgaccgc tcttttcata gtaaatttcg ctcccatctg gcatttttgc
    11701 atatttcttt tcttttttca cataatcacc tactgtgtaa agtataacga ttatatcagt
    11761 cattgggcaa ttgtttttac tgaaaaacaa cgatcatttt cgtaagatgt caaaaaatac
    11821 aggaaaattc attttttctt aaggcgtaca cagaaatcaa ggaagaaata gccaagaaga
    11881 taaagaaata gtaagataaa aaaagatgtc aaaataacta ggtgatgaaa tgaagcgaaa
    11941 acgattatct attatccgta ttctttttta catgatcatt ctgacggtta ttggtgcggt
    12001 gttgttttta tgaggaagaa cttatcgttc ggtaaaacga gtggaaagct atcaaataga
    12061 aataacaaca gctgcaaaaa attatcatat ggaaaactac gagaatttga tcaaggcgat
    12121 aatattgacc gaatcaaaag gaaaagggac agatctcatg cagagctcgg aaagtctgca
    12181 tggagaagct gagatcattg ataatccagc tgacagtatt gatcaaggtg tcaaatattt
    12241 ggcggaaatg atagtagaag caaagaaaca aggatgcgat ttatggacag ctgtccaagc
    12301 atataatttt gggaaagatt atattgccta tgtaaaaaat catggcggag aaaacacagt
    12361 agaattagca gaagcgtatt ctaaagatgt gctgagccca cttttaggaa atgaggaagg
    12421 gaaacaatac cgatattggc ggatccagtc cttgctttac aatggcggct atctctatca
    12481 taatggaggt aacatgtttt atcctgatat tgtcaaaatg aatgaaaaaa taatagagac
    12541 ctatgaaaaa gtctttaagt aaaatagcag gaaatgttca atcatgaata aaattaaatg
    12601 aaagaaggag tgcgcaaata gtggaaaaaa ggcaaaaaat ggaaaatcaa gaagtggggt
    12661 acacaaccaa agactggatg ttacggtttg tgaagggaat gtttatcggt tcaggattta
    12721 tcttgccagg agtcagcggt ggtgcattag cagctgtctt tggcatgtat gagcggctga
    12781 tttctttttt agcccacctt acaaaaaatt tcaaagaaaa tgttttgttt tttctgcctg
    12841 ttggtctagg cggagtgaca ggcatctttc tactttcttt tttagtcagt tttttattag
    12901 gtgcgtatga aacaacgata ttatggtttt ttgtcggatg tatcgctgga actgttcctg
    12961 cgttatggaa agaagcaggg aaaaaaggaa gagatacttc tgatgtgatc atcatggttg
    13021 ttacttttgt tctggcttat ttgtttttat tatacggcgc tcaattattt gacgggcaag
    13081 tcgatcagaa ttttggaaca tggctgattg ccggcggatt gattggtttg ggtatgatcg
    13141 tcccaggatt gagcccttct aattttttgg tgtatatggg attgtataaa gcgatggctg
    13201 acggatttaa agaagtacga cttgaagtga ttgtgccgat cggaattggt gggattcttt
    13261 gtgttcttgg attgtcaaaa atcatggatt acattttctc aaaggcatat agcaagctat
    13321 tccatttcat tttaggtgta gtttttgctt caacgattat gatcattcct accgattacg
    13381 cgggattggg agttgctggg gtactagctt gtcctgttct ttttatagcc ggtacagcac
    13441 taggttggtg gatgagccgt ttggaggaac aatataagta aagatagatt gataataaaa
    13501 acagcacacg ggtgattcaa acaaaaaggt tggcatcttt tttgtaaaat cattcgtgtg
    13561 tttttctata aattttataa aaacaagttg tttttaatct tggaacacgc tagacttaaa
    13621 cacgtgatta tttttgaaag ttggcggaaa atggagaaaa agaaaatgaa tggacttaca
    13681 cagcaagagg tagttcagaa aatggcttct ggccagcaaa atgactacca ggaagatgca
    13741 tcaaaaagta caaaacaaat attcagcgat aatatattga cgttattcaa ttttctgaac
    13801 tttggtattg ggatttgtct gttatttgta ggtgcgtatt ccaacatggc gtatttagca
    13861 attattgtcg tcaatatcgt tattggaatt tatcaagaaa tacatgcaag aaatcttgta
    13921 cggaaattat cgattgtttc ccaatcagaa gtactagtga tccgggacgg taaagaacaa
    13981 tcgatttcta ctaaagagct ggtattagga gatttggtaa aaatcggagc aggagaacag
    14041 ataccagccg atttacaggt tatatctgga cgggcagaag caaatgaagc tcttttgaca
    14101 ggcgaatctg atttgattgt caaagaaact ggagctgaat tgctttctgg aagctttttg
    14161 acaagcggtt ctgtcatggc tgaagttatc cgagtaggtg cagataatta tgctgtccgc
    14221 ttgactagcg aggcaaagac tcacaaagct attcgttctg aacttgtaga ttcgattcgc
    14281 aaagtatcca aattcactag tttcgtgatt attcctttgg ggatcatact atttttggaa
    14341 gcactgttca tccgcgatgc ttctatcaaa atttctgtta ttgcatctgc agcagctttg
    14401 ttaggcatgc ttccaaaggg tttggtattg ctaataagta ttgcattaac gacaggggta
    14461 accaaactgg caaaaaaacg aatccttgtt caagacatgt attcaattga aacgctggcc
    14521 catgtcgata cattatgctt ggataaaaca gggaccatta ctgaagggaa aatgagtatc
    14581 cagcgggtag aagcactaca tccgctgtac gagaaagcca tcccagaaat tatggggacg
    14641 tatctggcag aaagcagtga taacaacatc actatgcggg ctatgagaga ttattttgag
    14701 ctttctaatc ggtatgatgt gacaaacaaa gtcgcatttt cttctgatcg caaatggggc
    14761 gcgatggaat ttccagaact gggagtcgtg tacgtaggag cgcctgaacg tttggtggaa
    14821 gaagatcggc ttccggacag tctaaaaaca gcccaagcga atggctatcg cgtacttatg
    14881 ttggggatcg ctccaaatca gcaattgaac gaagaaaaac cagcatttgt tgagccggtt
    14941 gctctgtttg aaatcgatga tccaatccga aaaaacgctc aagaaacact agcttatctt
    15001 catgaggaag gtgtggatct gaaagtgatt tctggagaca atccttttac tgtctccaat
    15061 atcgctagac gtgcaggatt accgaattat gatgcgtatg tcgatctttc tcaaattact
    15121 gaagaaatgg aagtgcgaga agctgctcat cgctatacgg tttttggacg agtgagtccg
    15181 cagcaaaaga aattgctggt aaatgaattg aaagaaagcg gcagaacggt tgcaatgaca
    15241 ggcgacggtg tgaatgatgt tttggcgtta agagaagcag attgcagcat tgctatggca
    15301 gaaggtgatg gagcaacacg tcagattgcg aatctggtat tgttggactc tgattttact
    15361 acattgccag aagtcctgtt tgaaggacgc cgagtggtca acaatgtgac gaaagtctca
    15421 ggcatctttt ttatcaaaac gatttattca tttattttat cgattatatg tgccgttaca
    15481 gcaattggat ttccgtttat ccctatccaa atcacgctga ttgatttagc gattgaaggg
    15541 tatccttcct tctttttatc ttttgaacaa gataaaagaa aaatcactta ccgttatttg
    15601 cctacagcac ttattaatgc tttaccaaat gccttgcttg tagtagtgaa tatcattgcg
    15661 gtgtatctac ttggaaaaac attcgcatgg ggaaatgctg aaactactac tttgatgtat
    15721 tacttgctga ttgggatcag ttgcatggca gttgtaaaag cttgttatcc cttcaaccca
    15781 ttgcgcatct tcctagcagt aacgacgatc gtagggatat atgtagctgc aatgctgttc
    15841 catggaattt tggaagtaaa cttagggttg ggagagagcg taggatactt ctttgtattc
    15901 atgctcgtaa atattgttct tcgagtattg tactggaagg ctgatattca aaatcagtta
    15961 ctgaaaagag tagcaaattg atgcttaatg agatagaaat gtgttatact tgaaaaagca
    16021 atgaaatatg atacaatcaa acatgtataa ttgtgtcctt taaaatcgca ctgatttcag
    16081 ttagtgcgat tttttaccgg ttattctcct gaaatatctt ctgttaaacc caaaagatat
    16141 caatagagca gggggtgaaa aaatggctca acggattcct caagaggtta tcgaaacagt
    16201 acgcagtcaa acgaatatcg ttgaggtgat tggacaatat gtccagttga aaaagtctgg
    16261 taaaaattac ttggggctgt gtccttttca tgaagaacgt acaccgtctt tttctgttgc
    16321 agaagacaag cagatttttc attgctttgg ttgtggtaaa ggcggaaacg tgtttacttt
    16381 tcttcaagaa ctagaaggtc taagttttcc agaagcagtg gtaaaagtgg cagacttcga
    16441 acagataccg ttagaagatc aatggcgtca gcgtactata gctgtccaaa atcctgaatc
    16501 tgctacgggg aaactgattg ctgctcatga aaaagcagcg gaaatctatc atcacatgct
    16561 attgcataca aaagctggac agccagcttt agaatatttg caaagtcgtg ggttgtcttt
    16621 agaattaatt gaagaattca acataggttt tgcacctaat gagcgttctt ttcttcagca
    16681 agtattcaag aatgaatcaa tttcaaatga attgttagaa cgctctggat tgtttgtcac
    16741 gcatgatgat ggccgtatgt ctgatcgttt ttatcaaaga gtgatgtttc cacttcgtaa
    16801 ttctcaaggc cagacgatcg gtttttcagg acgctggctt gcacctgaaa aagggcagga
    16861 aaaagatcag cccaaatact tgaatagtcc agaaacagaa ctgttcaata aaagacaagt
    16921 tttatttaat cttgataaag caagaagcga tatccggaaa aacggagaaa ttttgttgtt
    16981 cgaagggttt atggatgtga ttgctgcatg gcaggctggt atcaaaactg gattagcttc
    17041 tatggggaca agtctaacag ctcaacagat ccaacaaatg gaaaagctga cgaaagaatt
    17101 ggtcttttgc tatgacggag acaacgcagg ttttgaagca accaatcgag ggattgaatt
    17161 gcttcggcaa aacagtcgat tagcactttc tgtcgtagtc gttccagaaa agcttgatcc
    17221 tgatgaatat ctccgtaagt atggagaagc atctttccaa gaattagtgg ctcatgggcg
    17281 cgaaacagtc tttactttta aatcacgtta tttaaaacaa gggaaaaatt tagataatga
    17341 aaaagacaag atcgaatacc tcgaagaatt actagttgaa cttagccgag ttgtttcacc
    17401 aatcgaacaa gatatgtatc tatctcaact gtcaacagag tttcagatcg gcagagagac
    17461 gatacaaaaa caactgcgcg atcttcgaag gacaaatcag caaaatcgtc aagaacctca
    17521 ggaaagtcaa cacactcagg ttacgagaca agaaactgca cggaaaaaga gacctttgac
    17581 gcaagtagaa aaggcagagc agattctatt atatcgtgca tttaaagaag tttctgtccg
    17641 taatatttta aaagaacgag aatttcagtt tattcatgat gcatatgcag aagtttattt
    17701 tttattcgat gcatatgtct cacaacatta tgaattcaac cttgcagagt ttttagactt
    17761 tttgcaagat gaaaatttaa ggcggttggt ggtggaaatc gaatatttac cggtggcaga
    17821 agaaagttcg ccacgagaaa tccaagactt gctagatgtg atcagcaaat ctggcttagc
    17881 tgatgaaata acattaaaaa aacaaatgca gcaagaagcc agacgaactg gaaacaaaca
    17941 gcttgagctt gaattgacga ttgaaatcat caatttaacg aaacagttaa aacaagccaa
    18001 atagctactc aaggggggcc ttcttttatg gcaaatggaa cagataaaaa aacgtatgaa
    18061 gcagcagtag cagctttcat caaagaaaac aaacccaaac gacaagttgt atatgatgat
    18121 ttatccaatc agcttgctac accattcact ttgaatgcag atgaaatgga caaattgatc
    18181 caaaagattg aagacgcagg tatcagtgtg gtggatgaaa atggtgatcc atcaattcac
    18241 agcttgaaga gtgctgagaa aaaagctgaa caagcaaaaa cagaagatct ttctgcgcca
    18301 accggtgtaa aaatcaatga cccggttcgt atgtatctga aagaaattgg ccgtgtctcc
    18361 cttttaactg cggaagaaga agtagcgtta gcattgaaaa tcgaagaagg cgaccaagaa
    18421 gcgaaacagc gtctggcaga agcaaacctt cgtttagtag taagtatcgc taaacgttat
    18481 gtaggacgag gcatgcagtt ccttgacttg atccaagaag gaaacatggg actgatgaag
    18541 gcagttgaaa aatttgacta ccgcaaagga tttaagtttt caacctatgc tacatggtgg
    18601 attcgtcaag ccatcactcg agcaatcgct gaccaagcac gtacgatccg tattccagtt
    18661 catatggttg aaacaattaa taaattgata cgtatccaac gtcaattgct gcaagacctt
    18721 ggaagagaac ctacgccaga agaaatcggt gcggaaatgg atcttccaac agaaaaagtc
    18781 cgagaaatct tgaaaatcgc gcaagaacct gtttcccttg aaacgccaat cggtgaagaa
    18841 gatgactctc acttaggtga tttcatagaa gaccaggaag caacaagtcc agcagaacac
    18901 gcagcttatg agttactaaa agaacaattg gaagatgtac tagatacatt gactgatcgg
    18961 gaagagaatg tccttcgctt acgttttggt ttagatgatg gccggacgag aacattggaa
    19021 gaagtaggga aagtatttgg tgttactcgg gaacgtattc gtcagatcga ggctaaagca
    19081 ttaagaaaat tgcgccatcc ttctcgttca aaacaattaa aagatttctt agaataagcg
    19141 tggactattg accagaagag aaatcagtaa aactgtttgg tttattttaa aagaaaaaaa
//
LOCUS       DACDMM010000042        19120 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDMM010000042 DACDMM010000000
VERSION     DACDMM010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.042"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..703)
                     /locus_tag="IWD16_001126"
     CDS             complement(<1..703)
                     /locus_tag="IWD16_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9088205.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="IWD16_001127"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="IWD16_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="IWD16_001128"
     CDS             1758..2837
                     /locus_tag="IWD16_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9088206.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="IWD16_001129"
     CDS             2869..3462
                     /locus_tag="IWD16_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9088207.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="IWD16_001130"
     CDS             complement(3572..3799)
                     /locus_tag="IWD16_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9088208.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="IWD16_001131"
     CDS             complement(3902..6490)
                     /locus_tag="IWD16_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP9088209.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="IWD16_001132"
     CDS             complement(6510..7169)
                     /locus_tag="IWD16_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9088210.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IWD16_001133"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IWD16_001133"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP9088211.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="IWD16_001134"
     CDS             complement(7942..8808)
                     /locus_tag="IWD16_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9088212.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="IWD16_001135"
     CDS             8917..9558
                     /locus_tag="IWD16_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP9088213.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IWD16_001136"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IWD16_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP9088214.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="IWD16_001137"
     CDS             complement(10999..11898)
                     /locus_tag="IWD16_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9088215.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="IWD16_001138"
     CDS             complement(11900..12697)
                     /locus_tag="IWD16_001138"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP9088216.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="IWD16_001139"
     CDS             complement(12675..13379)
                     /locus_tag="IWD16_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP9088217.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="IWD16_001140"
     CDS             13510..14085
                     /locus_tag="IWD16_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP9088218.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="IWD16_001141"
     CDS             14203..14880
                     /locus_tag="IWD16_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9088219.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IWD16_001142"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IWD16_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP9088220.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="IWD16_001143"
     CDS             complement(16943..17941)
                     /locus_tag="IWD16_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP9088221.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="IWD16_001144"
     CDS             complement(18167..18820)
                     /locus_tag="IWD16_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP9088222.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5522 a         4136 c         3323 g         6139 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt
//
LOCUS       DACDMM010000043        19018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDMM010000043 DACDMM010000000
VERSION     DACDMM010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.043"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWD16_001145"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWD16_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP9088223.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWD16_001146"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWD16_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP9088224.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWD16_001147"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWD16_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP9088225.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            1642..2274
                     /locus_tag="IWD16_001148"
     CDS             1642..2274
                     /locus_tag="IWD16_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9088226.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="IWD16_001149"
     CDS             complement(2276..3181)
                     /locus_tag="IWD16_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9088227.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3919)
                     /locus_tag="IWD16_001150"
     CDS             complement(3266..3919)
                     /locus_tag="IWD16_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9088228.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="IWD16_001151"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="IWD16_001151"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP9088229.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="IWD16_001152"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="IWD16_001152"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP9088230.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="IWD16_001153"
     CDS             6168..7253
                     /locus_tag="IWD16_001153"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP9088231.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="IWD16_001154"
     CDS             7250..8314
                     /locus_tag="IWD16_001154"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP9088232.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="IWD16_001155"
     CDS             complement(8563..9366)
                     /locus_tag="IWD16_001155"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP9088233.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWD16_001156"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWD16_001156"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP9088234.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="IWD16_001157"
     CDS             complement(10244..11251)
                     /locus_tag="IWD16_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9088235.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="IWD16_001158"
     CDS             complement(11258..11902)
                     /locus_tag="IWD16_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9088236.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="IWD16_001159"
     CDS             complement(12002..12760)
                     /locus_tag="IWD16_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP9088237.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWD16_001160"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWD16_001160"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP9088238.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IWD16_001161"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IWD16_001161"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP9088239.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IWD16_001162"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IWD16_001162"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP9088240.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IWD16_001163"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IWD16_001163"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP9088241.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IWD16_001164"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IWD16_001164"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP9088242.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..>19018
                     /locus_tag="IWD16_001165"
     CDS             18158..>19018
                     /locus_tag="IWD16_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9088243.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRK"
BASE COUNT         5338 a         4157 c         3497 g         6026 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaa
//
LOCUS       DACDMM010000044        18818 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDMM010000044 DACDMM010000000
VERSION     DACDMM010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.044"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(123..1631)
                     /locus_tag="IWD16_001166"
     CDS             complement(123..1631)
                     /locus_tag="IWD16_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP9088244.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1790..1981
                     /locus_tag="IWD16_001167"
     CDS             1790..1981
                     /locus_tag="IWD16_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088245.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2184..3101
                     /locus_tag="IWD16_001168"
     CDS             2184..3101
                     /locus_tag="IWD16_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP9088246.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3094..4167
                     /locus_tag="IWD16_001169"
     CDS             3094..4167
                     /locus_tag="IWD16_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088247.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4315..4833
                     /locus_tag="IWD16_001170"
     CDS             4315..4833
                     /locus_tag="IWD16_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088248.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4814..5206
                     /locus_tag="IWD16_001171"
     CDS             4814..5206
                     /locus_tag="IWD16_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9088249.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5282..6217
                     /locus_tag="IWD16_001172"
     CDS             5282..6217
                     /locus_tag="IWD16_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP9088250.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6350..7129
                     /locus_tag="IWD16_001173"
     CDS             6350..7129
                     /locus_tag="IWD16_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP9088251.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7122..7439
                     /locus_tag="IWD16_001174"
     CDS             7122..7439
                     /locus_tag="IWD16_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9088252.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7683..9293
                     /locus_tag="IWD16_001175"
     CDS             7683..9293
                     /locus_tag="IWD16_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9088253.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9450..10298
                     /locus_tag="IWD16_001176"
     CDS             9450..10298
                     /locus_tag="IWD16_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9088254.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            10529..11008
                     /locus_tag="IWD16_001177"
     CDS             10529..11008
                     /locus_tag="IWD16_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088255.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            11041..13269
                     /locus_tag="IWD16_001178"
     CDS             11041..13269
                     /locus_tag="IWD16_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9088256.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            13398..15041
                     /locus_tag="IWD16_001179"
     CDS             13398..15041
                     /locus_tag="IWD16_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9088257.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(15114..15659)
                     /locus_tag="IWD16_001180"
     CDS             complement(15114..15659)
                     /locus_tag="IWD16_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP9088258.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(15682..16167)
                     /locus_tag="IWD16_001181"
     CDS             complement(15682..16167)
                     /locus_tag="IWD16_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9088259.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(16157..16969)
                     /locus_tag="IWD16_001182"
     CDS             complement(16157..16969)
                     /locus_tag="IWD16_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP9088260.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(17108..18220)
                     /locus_tag="IWD16_001183"
                     /pseudo
     CDS             complement(17108..18220)
                     /locus_tag="IWD16_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
     gene            complement(18241..>18818)
                     /locus_tag="IWD16_001184"
     CDS             complement(18241..>18818)
                     /locus_tag="IWD16_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9088261.1"
                     /translation="LTKGELVGIIDSFPSQLCKPVRNRQAKLLNQIAKVGYNSTKKSY
                     FYGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEK
                     LALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLE
                     SRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         6106 a         3419 c         3745 g         5548 t
ORIGIN      
        1 agaaaataaa gcgaacaaaa taaaaggttt aaaaagtgta acgtcttagt aaagtctata
       61 aaaaaacgaa actatcgatt aagctaatag tttcgttttc aacatttata agtaaacttt
      121 ttctattggt aatatagatt ttcggaaggg tagacaggtg cgcaggtaat atcgattcct
      181 agttttttca atgtttgttc atcattttta ttgaccatca cagtagagtg ggcttgtgct
      241 ccctcaagtt cagctagttt atcataagca acttgagcga ttggattcgt tactgcactg
      301 attgataaag cgatcaagac ttcattgatg cttagtgcat ccaaagagga atgaagtgtc
      361 tttgatttca atccttgtat tgtttcaaga actaatggtg acaataaagg aatgtcatct
      421 gcgatatggg ctaaatgttt tactgcattt aagataactg ccgctgacgc atccatcaat
      481 gaacttgtac gcccggtaat gatgacttca tctggtaatt cgattgcaat cacagcagaa
      541 ggatcttttg taggactttg cgcttgaatc ttatctgcat agtcatgggc agggactacc
      601 acgtttcggt cttcaggacg taaaccgact tcttccataa tcagtttgat tcgattaact
      661 gcttcttcgt cgatcaatcc ttttttgaag tcgcattctg tttggaaata acgacggatg
      721 atttcttgtt tagaggcttc ttggacaacg gtatcatcca cgatcccaaa tcctacacga
      781 ttcacaccca tgtcagtagg agattgataa acagattctt taccagtaat tttttcaata
      841 atacgtttga ttacaggaaa tgtctcaaca tcccgattat agttcacagc tactttattg
      901 taggcatcaa agtggaagga atcgatcata ttgacatctt ttagatcgac tgtggctgct
      961 tcataagcaa tgttcagcgg atgtttcaac ggaacattcc atactgggaa agtttcgaac
     1021 ttggagtaac cagctgcatg tcctttttgg ctttcatgat acagttggtt caagcaagta
     1081 gcgagctttc cacttcctgg gccaggagct gtcactacaa cgattggttt agttgtttca
     1141 atatactcat tttttccaaa accatcttct gagacgattt tttctacgtt gactggatag
     1201 tcttcgattt ctgagtgttt atagaccttg atgtttcgtc tttctagttt attgataaag
     1261 actttcgtgg caggctgtcc actatagcga gtgataacga cactgttgat taataagcca
     1321 taaccacgta attcatcgat taaccgcaag atatccatat cataagtaat cccataatca
     1381 ccacggatct tattacgttc aatatctcca gcatagacgc aaatcagtat ttcagcttga
     1441 tctttcaatt tttgcagcaa tttgattttc gcatcttctt caaatcctgg aagtacacgt
     1501 ttcgcatgct tgtcgtcaac taacttccca ccaaattcta aatataattt gtcatagtgg
     1561 ttcactcgtt ccaaaatata cgctgactgc tcttcaatat atttttctga atcaaaacca
     1621 atttttttca ctaggttccc tccttaagta ctaaagcatt ttttcatttt tttgaggatt
     1681 cgtcaactgg aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa accaacttgt
     1741 ctagatagtt attcattggt atgatagttt ttgataagga aggtgctaca tgaaaaaaag
     1801 acagaaaaag aaaaacgcat ataaacacta tatccgttcg atttttaccg gttatgaaag
     1861 aatgcttgaa gatcccgaat tggaacaatt gacgtttact tatctaaacg aagaaacgca
     1921 gctaactcgt gatgagcatc agcgcattca tttcacaaca agagatctgc ctagtaaata
     1981 aaaaataaga taaaggtcat cggattcgat tctgtcctat cagatggaaa gactagttga
     2041 tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt tatggagcat tgcccgctct
     2101 tttttataac aattttttac agaaaatggt actattgaag tattaataag tgaaaaacag
     2161 atgagtaaga ggaggcaagt agtatgaaag agccagatac aaatgcaaat tatgagccag
     2221 acgaactaga ggagacgaac gcggatcagg atacaggaca agaatcgtct tctttttacg
     2281 atatcaaaga aaaagcaagc atgaatcttt cccaattatt agaagactta caggcttttg
     2341 aaaaagctgt ccgcgaagaa gatatccctg agatctaccg tttatacaat ggccgattga
     2401 atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg ctgatgcgta
     2461 agatccatga tcgtttttta caggaatttc cttttatgga acaagtagaa aacatctcgc
     2521 caacgatgag ctattacaaa atcggcactt attaccatga tcggcccacg gtcggtattg
     2581 atgcaagtct gccagaaatt tttgttttgc ctaagatcga tgaagagtgg gagaaatatt
     2641 cccaaccgga gtcaacggac tatgacaaaa aaatcaatga gctagacgca aaggtgatca
     2701 ctgctcagac agaaattgaa cgtttagatg aacaaataaa agaaatcaat cgacaaatga
     2761 ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa gaaattcaag
     2821 aattagagaa gaagaagcaa gtactatcta atgaaaaatt aggctggctt ccttatattg
     2881 agacgcctga aacaatccag cagcaaaaag aagcattgaa gcaagaagca agagcagacc
     2941 agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat cagacgctat ttcggtagca
     3001 aagagggatt cggtcaagca atccatgaat tcttgatgaa ttatcttggc aatgagaatc
     3061 ctaataatca atctaatgaa ggaggttccg agtatgaatg aacacgatga agcagaatgg
     3121 acaaaaaatg aagaacaaac agaagatcat gaagaaacga acacttcatt ggaagaacaa
     3181 acaagtaatc ctctggatga cacagaagaa atccctatgc tagaggaaac aaaaatacac
     3241 gaaaaagaaa aagtaactcc gacgaatacg aatactgcag aaaacaccca tgcttacgaa
     3301 ataactgaaa atcccgggta tttagaagga aaagagcaag atcatcaaga tatcgtcagt
     3361 ttgattgaaa cagcggaagc agaattagcg gatttacgta tgagaaaaga tttgatcgaa
     3421 tcagattttc cacaattatt agaatcatat gatcgtttta tattgaatga agaaccaatc
     3481 tccacgatcg gaaagacaag cctgatcgaa tacttcacgt atgaattact gaaaccgttg
     3541 aatgatattg gtctggaacg gtcaaattca gcttatgaca catggaaaac aagacatttt
     3601 tcgatcaatt atacgttgaa tggacaaaac gaattagtct tttcctttgt attaccgacc
     3661 atcgatcaac gataccaagt ggaatcgatg agtctcctaa acgtatcacc agagacaatg
     3721 gaaatcaatg tccaagatga ccgggtgctt tctttgatcc gacactggtc agtggatcga
     3781 gtattctctg cagggcagat cacgatattc aatcacaaat tgaatcaatt attagcccat
     3841 gcaagaacac ttggttttac cgttaatcag acattactag acaatacgaa gccacttcgt
     3901 ttgaatcttc agagcgaatt tgaattgacg aatgaagtat tggatgatat ttttatcgtg
     3961 gcaatgaaaa atccatccta cgatatggaa aagatcaacg aaactactta tcaagtacga
     4021 ttagataaag gacagtcttt aacgataaca acaaacgaaa aagatcaaac agtcttgtcg
     4081 atctcatctg gagactaccc acgatctgtc attgatttct ttatcaatta cgaattcttg
     4141 gtcccattaa tggtgagaaa gtcctgattg gataagttgg attaccagat gtcattcaga
     4201 taattctgtt ccaaaggact tgcggcagtt atccagaata attgtcacaa gtcttttctg
     4261 tatgtactaa atggacgtga ataacttgct gatgaaggag ggatttcatt aggaatgtcc
     4321 acaaataaaa tagcacagct tagcttattg tctgctgctt gtgtagcagg taggatcgct
     4381 tttcaattta ttccgaattt ccagccaatg actgctattt ttttatttat agtactttac
     4441 ttaagcttaa aagatgcgtt aattgtcatg agtctttcta ttgcgatcag cagtttttat
     4501 tttggtgttg gaccatgggt catcggccaa tggatcagct atactgttgt tctgagtttg
     4561 ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc tatggttaaa gggagtctgt
     4621 ttttttctgg cgggcatggt ttacggaata ggtatgaccg tttttgatac gctgttatat
     4681 cgtttgcctc aaccttggat ctattacctt caaggggtgt cgtttgattt gatgcacagt
     4741 ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat
     4801 tcaggaggaa aaaatgaaaa aaataattta tagtttgctt gcaatgagtg tcagtatact
     4861 cattttgaca agttgcagta caacctatac acaatcggct tccaaccaaa aggaggaagt
     4921 aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa
     4981 agaagtgttc ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa attttgaagt
     5041 caaagaagat aacggcttca tcacttcgct tgaagggcac tcacaggatg agaaaaatca
     5101 actctattgg atgtttactg tggacgggaa aagtgctact actggtgcca aagagatcaa
     5161 attgaatgat ggacaatcgg tcattttcaa tttgtctaaa ctataagttt cataaaaaac
     5221 tgttaaaggg cgaatattgc cataattcag tgagttcagt tgatgaaaga aggaaaataa
     5281 catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag gggctaatat
     5341 tctagttgct atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa
     5401 cgaaagcatc cacagtatcg ttgattgcag caatcagatt ttgctgttat tcggtgataa
     5461 acaagccaat aaaggacaga gcgagttgca tcaatttggt gaaggacgag caaaatactt
     5521 tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg gggtgatgga
     5581 agcttttgaa aagttgacgc atccatccca tgaagtgggt aatccgatca ttgttatagc
     5641 aatactgtta tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat
     5701 caaagaattg aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag
     5761 tgaaatactg attattttta cggaagactt gtgtgctgta atcggtttgg tcttggcttt
     5821 agcaggaaca atattgactt atgcaaccgg gattgctgca tttgatgcga tcagcggatt
     5881 gctgatcggt ttcttgctga tgggagccgc cattttccta gcaaaagaat tttatagtct
     5941 gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa atcaagttag cttttgaacg
     6001 tcctgagatc agtcggctga tcgatataaa aacagttcat ttaagtccaa cggagatttt
     6061 agttgcagca aaagcagata tcgttggtaa caaagagaat gaagcttact cggtcatcaa
     6121 tgatattgaa aaaaatatgc gtcagtctct tcctgacaag aagatgtata tatacattga
     6181 aacagataaa tatgacccaa attattctcg taaataaaac gtttattcat gaattgaagc
     6241 aaaaatgaga aaaatataat cttgttttta aataaaacgg ctacaattta gcgaatttat
     6301 gttacaatga gtccgctgtt tatttgttgt aattaaagga gatattttaa tgaacaaaaa
     6361 gaaaagtgcg ccatggcttc aagctttgca tgcagtgatg ccactttgta taagctatat
     6421 accagtagga ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc
     6481 aggactgtta tcgattctgg tcttttccgg tggcgcgcag tttttagttg catctatgct
     6541 tacgacgcaa gcatcatttg caacgacatt attgatggtt ttctttttag aattacgcta
     6601 tacattatta ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc
     6661 tgtcttttct caatcactga atgatgaaaa ctatgcggtg aactacttaa agttttcaac
     6721 tgacccttcc tggaataaaa gaaaggcttt atatgtcaat tggttctcaa tgactgcatg
     6781 ggccggtagc aatatgatcg gtaatttttt cggctcggtg attcgagtag atgctgacct
     6841 tgtccatttt gctttgacgg ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc
     6901 actactgatc tttacaggtt tattctcagg cgtattaggg gtcgtattca tggtattatt
     6961 ccaaaatact ttcggattga ttgcagcaac agtcattgct tcatttgccg gatttatgct
     7021 ggagaaggca ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa
     7081 tgagccgctc ttcaacttcc cggaacagga ggccaatcac catgactaaa ctatatttgc
     7141 tacttttagt tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt
     7201 attttactca tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat gtaccggtgg
     7261 cattatttgc ggcgttagct tttaaagatg ttttcattac tcatgaacat ctggatattg
     7321 cttggaatat caaaatagct gcaatgattc tagtagcagg cgtggcttac aaaacacgtt
     7381 cgatggcgtt gtcagttttg acaggacttg cgtctgtctt tttgctgtct atgctttaaa
     7441 ctgttttatt ttattaaaaa cagagattgc gacataagtt gttcaaactg atctatccga
     7501 actgcatttg caacagtcaa ttctggatgc aagtttattc gtagggataa aaaagattag
     7561 tgttggctta tgatgcaagc tcttttttat tttaggaatt agagagggat ttgtccttct
     7621 atgatttttt gctataatag ggaagatgtt tattttatga aagaatagtt gagggaattt
     7681 aaatgaataa aaaattgatg caaggaacat tttggctaac ctttgctaat cttctatgta
     7741 aagttttagg ggtagtctat ttgattccct ggctaagtat gatggggaac aatcaagatg
     7801 gtatgcttgc tacgacattg tataatgttg gctatcttcc ttatggattg ttcctgatgc
     7861 ttggtacagt cggatttcca aatgcgattg caaaaaaagt agctgtagcc acaaaagaag
     7921 gagataacgc tgcttgtcgg ctgattttta gaagtacgat caatatcatg tttgtcatcg
     7981 gtatcgcctc tgctgcactg atgtatttat ttgctccttt actagccgga atcagtccga
     8041 ttgctaacgt taataatggg atactagcta tcagaagttt atgcccatct ttgattgcta
     8101 tcccaattct tagtgcaatg agaggatatt tccaaggaaa aaatgatctg cggccttatg
     8161 gaacttcttt gattatcgaa caggctgtgc gagtgatcgt tatcttggct ggtacttatt
     8221 acttgcgcgt attgactgac ggaaccatat tagaagctgt attgatcagt acagtcgctt
     8281 ctttcttcgg tgggttagct gcgattatcc atatgtatat cgtcggacgc agaaaagact
     8341 tcttcgaact taaagatttt ttgatttcca gccgttattt cgaaagagaa aaccgatctg
     8401 cctctgtctc tatcattcgg gaaacactac cgtttatttt tgtcggctca gtcattacta
     8461 tcgttcaaat gatcgaccaa gtaacgatga aaccactgct tcactttttc cgtccagaga
     8521 tcgcagatca gcaactggaa ttcttattca gccgtgcttc tgtcaatcca aataaattga
     8581 cattgatcct gatctccatg gtaggaacag tagctataag cagcttgcca atattaagca
     8641 cgatgaagaa aaaagatcgg atacaaatcg aaaaaacagt aggagatagt ttttcgattg
     8701 cgttattgat tcttcttcct tcattgactg gaatgtcttt attagctgga ccactataca
     8761 cattgttttt tggctatgat cctgaaagtg tcggatattt ccaaatggct ttgctagctt
     8821 ctttgttttt ctctttattc acgatcctat ctacgatgct tcaatctttg aatcaccatc
     8881 gttttgcgat ccgattaact gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg
     8941 gattaggatt agttggcgga tacgggatga gcttatcaaa tggcttagca ttcggtattg
     9001 tctttgttcg cggctatcac tttatgtgca aagaataccg aatagcacct ttagcaaaga
     9061 tcagcaattt tttcttaaaa actttccgct ccaccctgat tatgcttgca gtatgttttg
     9121 ccgttttctt tttgctgaat cagcgattgt caatggaatc aaaaagctat gcaatgattt
     9181 attgtgtggc tgtaggaagt attggcggct tagtattttt attttctcaa ttcggaaaaa
     9241 atgggtttcg aatgttgaaa aatgttaaac atcatagcag aagcaaagaa taggttaact
     9301 aatcgttttt tgctgtcact tgaaaaacgg caactcgtat gattacgctt gtcgtttttc
     9361 tattttccaa ttttagtttc atatctaatc ttacttatga tatagttata aggtctggaa
     9421 aaattctttg aaacgaggaa attaaacaca tgaaacttgc agtagttaca gatagttcgg
     9481 catatctgcc ggaacgtatc aagaaccatc ccgacttatt cgttattcca atccctttga
     9541 ttatggatgg gaaaatttat aacgaaggaa tcgatatcga agcggatgaa tattatggac
     9601 ttttaaaaaa cagtaaagat tttcctacta cttcacaacc tgtcgtaggg gaagttttgg
     9661 gtttatatga ggaattgaaa gagaaagggt atgacacgat tttaagtatc catctttctt
     9721 cagggatttc tggctttgtc aacacattat ttgcgatgaa agatgacatc agcggagtaa
     9781 cggtcattcc ttatgactct aagatcacta gtatgccgat gggacacatg gtagaagctg
     9841 ccttagactt aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa
     9901 tccgagataa tacctacgct tatctgattg tagatgattt gaacaattta gtacgaggag
     9961 gacgtttgac aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga
    10021 cattttctga agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg
    10081 ctcgagctga agaaattatt ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt
    10141 acgtgataca tgcaaataat ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa
    10201 aatatccaaa tgcagaaatc gaaattggtc atttcggacc agtcattggt acacatttag
    10261 gtgaaaaagc catcggcatt gccatttcag cgcaataatt ccttttattc aaaaaacata
    10321 ccaaaaatat tccaacatca tcttggactt tctacatgtt actaatgagc gtgtaaaaga
    10381 acgagatggt gtttttattt ctatcaatga agctcttttt ctttgcaatc ctcacttcaa
    10441 tcctctataa tgtcgcatat agtttagtaa atgagtaaac tactaattta cttatttact
    10501 aaaaataaaa ggatgaggag ttaaaataat ggaaatacca acaaatttaa aacataaacc
    10561 agtaatcgtt gtagaagact atgataaagt tgatggaaga aatgcattaa atacggatgc
    10621 aaaagggctt tcactgggcc ttgctcagtg gaatgatcgt ggaaaagtcg atatctcagg
    10681 caaggtatgg cgacatactg gagaaaaatg gtcacgacag tcggaagaat taccaatcac
    10741 acgtatttta gatttagcga tcttagctgc acaaggaagt ttatacttcc aagatgctta
    10801 tcgttatgaa aaattttatg atccagaaaa tccagtcgta gacatcattg gattacaagg
    10861 gaaccgaatg acggtagaag tagataccaa aaatccaaag atcgatgaag acatcatttt
    10921 gtattacgat acactacaaa aagacggaga actgattggg gaacgattcc gtattttaaa
    10981 acgtctttta gatgagctag gatattaata gacagagatg atcaaaaggg gaagagaagc
    11041 atggagaatt tctcactaac agaaaaccga accttttcac ggctagaaaa gcaaatgatc
    11101 tggaagaaac cgaaaagcca tcggacaagt gcagaagaat ggcggatcgc ctccgaaatc
    11161 aaagggaatt ggcaagatcc tgaaatgaaa atcagtaacg tacttttgga aggagatgcc
    11221 gggtcgggga agacacaatt agccaaagca ctatctgctg atctgcaact tccctataca
    11281 aaagtgacct gttttgcaga tatggacaaa tcagatgttt tcggagcctt attacctgta
    11341 gtagattcta ataaggaaca agaccaagaa ttactagaag ctatttacca aacagataca
    11401 cttgaagctg tactgacact gatccagaac cattacggca ttgatccttt ttctgctaaa
    11461 gaaaaattag cagagctagt tcaaaggatc gaagctgatt atccagatgc aatccattac
    11521 agctattacc cttctgaaat cgttcgagcg attgaaaaag gatatttatt agagattcaa
    11581 gagccgactg tcatccgaga tgcctctgtt cttgtggcac ttaactcagc gctcgaacca
    11641 aatgggatgt tgaatttacc gacaggaatc attcgtcgcc atccagattg cgtcattgtg
    11701 atcactacaa atcgaaatta ccaaggaaat cgcccattaa atgaatcttt aagagatcgg
    11761 atgcaacatg cggaaaaaat ggatcttcct gccttagaag tcatggcaga acgtgcgatg
    11821 gcgaaaacta tgatccaaaa acaagaactt ctcttaaaga tggcagagat tatccgtttg
    11881 ttggatgaaa ctgcaaaagc taacgccatc aaaggtgtag caggaatgcg ttcttatttt
    11941 tattgggcaa atacctataa gcaaggacaa gatttactcc agtcgattta tccaaaagtt
    12001 ttgtataaat tatccactga ttcagatgaa ttggcaattc ttgaacaagc tttgtctgac
    12061 agtggattgc tactggaact tcaggacata ttgagaaaag aaaaatgggg tcatcctcaa
    12121 tctgagcata caaaaggaag aaccattagc gccgaagaag caaatgaacg aaacatcact
    12181 gaaaatgctg aagaaattct gcgcttagat gaaaagaaat cgttgtctgc tgaagaacca
    12241 aatgagggag aagagaaaca agaagaaaca atagaaaaaa acgagattcc tcctcaaatg
    12301 caaaaagaac gcagtagcga tatcgaagga aaaaatcaaa aagaacaaca aacaggagat
    12361 gattctcaag aaatgtctgc ttctgacatg gaagcacatg ataaggaaat caagaagcaa
    12421 ctgaataaag aagcgcgaca aatcatgaag gaaacgattc atgaaaaaga aggtctcatc
    12481 gttcaccgac cagctttcca aggaaaaacg ccggaagcac gtgagctaca catgcaagta
    12541 atgccaatag tagaatcact tagccggcaa attttagagt tactggataa tgaacaaaca
    12601 gagacgtacc aaaaaggaaa atacgaaggt cagcggttca atgcaagcaa agtagcatat
    12661 ggtgatttga gaaattttga caaaaagaac ccgccacatg agcaaccttc tttagcggta
    12721 gctgtacgga tcgatgaatc tgggtcaatg atacgggatg atcggatcga agcggcaaaa
    12781 aaagcagcga ttgccattgc cgaattttct aagaaagtcg atatcccatt gttgatttac
    12841 ggagatacag cagaccgttc gtcaagagaa aagacttctt tgttttctta taaagaattt
    12901 gaagacggat ttcaatggtt aaatgaaaaa ttagtcacta tgaaaccaag gcaaaacaat
    12961 cgtgatggtg ctgcacttcg tttagcagca gaaaaattaa acagacaatc cgcaacaacc
    13021 aaactgttgc ttaatattag tgacggtcag ccaaaagcgt tgccggatta tacaggcaaa
    13081 aaagcaaaag aagatatcca gtctgttttg aaagaatatg aacgtcaagg aatcctattt
    13141 gtctccgcag ctattggcca agacaaagaa gaaatcaagt cgatctacgg tgaaagtcgt
    13201 tttgtagata ttactgactt gaatgaattt cccaagcaaa tgattcaact gattgcccgc
    13261 tatctatgat aaaataaagc tcaacgaacg cacttagcag attcgttgag cttttcgttt
    13321 ctagttaccg gtaacaagga tacttctaaa atagaatcgg ttataatcaa gagggaccgt
    13381 taaaggagtg aaacgatatg atacaagaac aaaaatggtg gaaagaagca gtaggctatc
    13441 aaatctatcc tgctagtttc aaagatagca atggcgatgg tcaaggagac atcaatggga
    13501 tccgtgaaaa acttaattat ttgaaagatc ttggtatcgg ctttatctgg atcaatccga
    13561 tctatcaatc tccatttgtc gataatggtt atgacgtaag tgactatcaa gatatagaag
    13621 agagattcgg aacgatggaa gattttgatt tattgctaaa agaagcacat gaattaggga
    13681 tcaaagtgat tatggatctt gtcatcaacc atagttcgga tcagcatcaa tggtttgaag
    13741 aatcgaaaaa atctaaagaa agcccgtatc gcgattatta catttgggtc gacggggtag
    13801 atggcaaaga accgaataat tggacatcga ttttcggcgg ttctgcttgg gagtattctc
    13861 atgaaactgg acagtattat ctgcatgttt ttgcaaaaga acaaccagac ttaaattggg
    13921 aaagcgagaa attgaaagaa gaactattca atatgatccg atggtggttg gataaaggga
    13981 tagatggatt tcgtttagat gcgatttctc atgtaaaaaa agatgaatat tcagtaaaag
    14041 ccacagagaa ccctttctca ccctttcaaa atgtctcagg aatcgaagaa catttgaccg
    14101 atttgaaaca tgtttttgaa gagtacgata tcatgactgt cggagaagca agtggtgtca
    14161 ctgcagaaga aggaccacaa tgggtaggga aagacggtta tttcgatatg atattcgaat
    14221 ttgatcatat ccatatctgg cagcaagaaa aagaagggca attagatgtc cttaaattga
    14281 aacttgcatt aagcgcttgg caaacttcat tggatggtat cggatggaac gctttatata
    14341 tggaaaacca tgatgttcct cgtgccgttt cagtgtttgg agatactcgt cccgattttt
    14401 gggctatgtc tgcaaaagca atcgctatga tgtatttctt tttgcaaggt acgccattta
    14461 tttatcaagg acaggaaatc ggaatgacga atatgccctt tgaatcgatc gatcaagttg
    14521 atgcagtaga ttcgaaaaga ctttataaaa ggctgcttgc ggaaggtaag acaagagaag
    14581 aagcattgga tatcattcgt gaaacaacga gagacaacag caggacaccc atgcaatgga
    14641 cttcggaaca atatgctggt ttttctacac atgaaccttg gctgatcact aatccaaata
    14701 ccaaaacgat caatgtagaa caacaagaat atgaaccaga gtcggtcctg caattctata
    14761 agaacatgat ccgcattaga caaaccaata aaggtctgat atacggttct tataaagaat
    14821 atcttcatga acatccacaa ctttatgtct atgaaagata tttagaagat gaacaatatc
    14881 ttattatggt aaacttaaca gaatcacttg ctgactatga acttccaaaa gaagcagatc
    14941 agtcctggac attactcttg tcaaattcct ctagcggaga atttgaagct aaaggcatac
    15001 tagcacctta tgaagctcgg ttatataaaa caaacaagta aaacttcctc gttaaagttc
    15061 attagttgga ctttgcgagg aagtttttca gattgctctg tcaattactc atttcaataa
    15121 ttcgctctag gaccaccgat atatttagga cgagagcgaa gggctgtcac gtgagcttgt
    15181 ccaagggttt tcaaacgagg tctgacaggt acttggacat gtttgatatg cataccgatt
    15241 gatgtgtcac cgatatcgat accggcttgt ccaactacgt gttccacttc gacaggatca
    15301 ttgaactggt caaaagcagc aacagaacaa gcacctccag catgtagtgc aggaacaaca
    15361 gagacaattt cgaaattttt tgcttctgcc actgttcgtt ctactactaa tgctcggttc
    15421 aaatgttcac aaccttggac agctagcgca atttctttag gatccaacaa ctcttttaac
    15481 gtacggatga tccattgacc aacttccgca ctggaatttt ttccaatatg tcctccaacg
    15541 acttcgcttg tgctgcatcc taaaacaaaa agatcacctt ttttcaagtg tgcctcagcc
    15601 aatatgtcat taactatttc agtcaattgg tcttttaatt ctttttctga caacttcatt
    15661 ttctaaaacc ttctttttca attaatattg cttccgtgct tgaatacgtt gaagacttgc
    15721 agttatagga aaggcaagtc ccattccaat cagattttgg aagatgtttc ctggtataga
    15781 agccactcca gccggccaat catataaaat ccaaccggcg ataccatagc cgatgatcat
    15841 aactaagctt cctaataaaa gccctaatcc ccacatacga gtagaagatt tgctgaaaga
    15901 accagcaaca tatccttgta acccgtggat cactaatgaa aaaactgccc actgcggata
    15961 tcctgaaatc aaatcgatca atagaccact ggaagcacca acaactaatc cacccgttgg
    16021 accataaaga aaagcagcgg tatagatccc ggcctcgcat aatgtcacaa atccattcgt
    16081 agccggtaca gggatcaaaa ttgttaatga aagggcaact gtcaacgcaa tcagcatagc
    16141 atagatcgtt aattgcttag ttttcatttc tcattcctcc tttttgaata ccgccagacc
    16201 agacacttcc ggtatgatcg ttcatcagca cgccattaga aatacaatgg gcaacaaatt
    16261 ttttactttc tgctactgct ttggtaaaat ctgcccctaa cacaagattc gctgttattg
    16321 cagaagaaaa ggcacaaccg gccccgttta ctgttgtagt gtcgattttt ttatgagaaa
    16381 atacctcata atgatcaatt gaatagagaa gatccacagc ttcatctcca gccatccttg
    16441 tgccgccttt gatcacaatg ttttttgttc ctgaagcagt gagttcttta gcgagatgga
    16501 tcatttctgt taacgaagag ggaagagctt gaccagaaag taaagcagct tctgccaaat
    16561 taggcgtaat catcgtaaca agtggaaaaa gttcttcgat gagctgcgaa atatacaatt
    16621 gattgatcac agcttcagtt tctttgaatg ccaaaacagg gtctaaaatg attgggatat
    16681 tttgttttgt ttttatgaag tctcgaacaa ccatgattgc ttcacttgaa ttcaataacc
    16741 caattttgat accatctaat gaagtcattt tatcaatcga aactaactgt cgtttaagca
    16801 attccaaaga tatagattcc agcataaatc gctcattaat atctagtgcc cctatagaag
    16861 tcaaggcgct tagtccgaat agaccaaaat tctcaaaagt ttttaaatct gtttggattc
    16921 ctccaccagc aaatggatca gaaccgccaa ttgtcaagat tcttttcata acattttcct
    16981 ccatgaataa gagtataacg aaattttcaa aaaagaaaaa cagccaattg gcatattttt
    17041 atctcatcca atttaaaaaa acaaaaagaa aagaaagacc aaaatggatc tttctttcct
    17101 taaatagtta ggacttttgt gattttaatt ctttctcgat ttgaagccgt tgcactttca
    17161 ttgtagctgt tcgcgggatt gcatcaaaat cacggatgat agggacgttc aagcgaggaa
    17221 gttcatggac ttgctcccac catcgattcc aatccatttc tttatcagga gctaaagcaa
    17281 taatcggttg tggagaatga tttttatccc ttacaatcac tacttctgcc aaaaaatcta
    17341 acgaatctaa taaaaagtct tcaatagcta atgtactatt gatcgtttcg atcaaatcca
    17401 cttgccgatc tttcaaaaat aggtggccat gcttatccat aaaaccgtaa tctccgctat
    17461 cccaccaatc gccataaaca ttttcctgga accgtgcatc ttccttgtaa taagtcaaag
    17521 cccgtccttt agacagtaat tgtatatgac catctgtgtt ttcaggtagg acattcccaa
    17581 ctgaatctgt aatccgagcg cttgtcatgt cttccagtcc aacgcccata tctcttgcat
    17641 cacttgtttt caaactctct agtgtatgag ccttaagaat cattggtccg cattcacttt
    17701 ggccatatac ttgaagaaat accgcatcct gtgccatcga cgttcgcaaa aatgtcttca
    17761 ttgtctggtt gtttatagca tcaaaagtgg agtgatagta tcggataccc gaaaaagctt
    17821 ctggatgctc cttagcagta aaacgccact gaacaaagtt attcggatgg gtctctagtg
    17881 caattggccg gtgagcttcc aacatatgaa caacttttga actttgtgcg tttgctaaag
    17941 gcatcatagg aaatcccatg gacatcaaag aagagatacc aatattgaat ctggaatgca
    18001 ctggagagat gtgaaaagcg attaatttct tttccgaaat ttttgtgaaa actgtttttt
    18061 gccatttcgt ccgccatccc atagaatgag cagaatgaca aataagttta gggataccag
    18121 ttgtaccaga agtatgggtc atataagaaa tttcattttt gtctaaaaat gattgagaaa
    18181 cagacaaagc aggttgttcc attaaataaa gaataggaat acccgtggtg ctagttaatt
    18241 ttaatatcct aaagaatatc tcaaagttcc ttcaaaacgt cgttgtgctc gacttaatag
    18301 aacactgtaa gccagtaata tgctttcaaa tctactctct agccctttaa cagaacgtga
    18361 gttgaaattt tgacaaccta atttttctaa ggaagaaaaa acagtctcaa ctgtttttct
    18421 ttgttttcca agtaagctgt ggtctagttt ttctgattta tccatgtttt tacgtggcgg
    18481 aacggatata gtaatgccct taagggcaag cttttcatgg attttatggc taatatatcc
    18541 tttgtcccct aggatattgg gaagattcgc ttcttccgat aaagtctcta acactttcac
    18601 gtcatggaca cctggattcg tgattgagta agtaatcgga aagccagtct tagtaacgat
    18661 catatgaatt ttaagaccat aaaaataaga tttttttgtt gagttatagc caactttggc
    18721 tatttgattc aatagttttg cttgtctatt tctaactggt ttacataatt gactaggaaa
    18781 actatctata attccgacta gttcaccttt ggtgagct
//
LOCUS       DACDMM010000045        18610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDMM010000045 DACDMM010000000
VERSION     DACDMM010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.045"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            8..232
                     /gene="nrdH"
                     /locus_tag="IWD16_001185"
     CDS             8..232
                     /gene="nrdH"
                     /locus_tag="IWD16_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP9088262.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            391..2550
                     /gene="nrdE"
                     /locus_tag="IWD16_001186"
     CDS             391..2550
                     /gene="nrdE"
                     /locus_tag="IWD16_001186"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP9088263.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            2600..3565
                     /gene="nrdF"
                     /locus_tag="IWD16_001187"
     CDS             2600..3565
                     /gene="nrdF"
                     /locus_tag="IWD16_001187"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP9088264.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(3654..4793)
                     /locus_tag="IWD16_001188"
     CDS             complement(3654..4793)
                     /locus_tag="IWD16_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9088265.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(5102..5815)
                     /locus_tag="IWD16_001189"
     CDS             complement(5102..5815)
                     /locus_tag="IWD16_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP9088266.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(6069..6770)
                     /gene="nagB"
                     /locus_tag="IWD16_001190"
     CDS             complement(6069..6770)
                     /gene="nagB"
                     /locus_tag="IWD16_001190"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP9088267.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            7004..8536
                     /locus_tag="IWD16_001191"
     CDS             7004..8536
                     /locus_tag="IWD16_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9088268.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(8727..9929)
                     /locus_tag="IWD16_001192"
     CDS             complement(8727..9929)
                     /locus_tag="IWD16_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9088269.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(10011..10700)
                     /locus_tag="IWD16_001193"
     CDS             complement(10011..10700)
                     /locus_tag="IWD16_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9088270.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(10806..11174)
                     /gene="rplL"
                     /locus_tag="IWD16_001194"
     CDS             complement(10806..11174)
                     /gene="rplL"
                     /locus_tag="IWD16_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP9088271.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(11227..11730)
                     /locus_tag="IWD16_001195"
     CDS             complement(11227..11730)
                     /locus_tag="IWD16_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP9088272.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(12140..12829)
                     /gene="rplA"
                     /locus_tag="IWD16_001196"
     CDS             complement(12140..12829)
                     /gene="rplA"
                     /locus_tag="IWD16_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP9088273.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(12933..13355)
                     /gene="rplK"
                     /locus_tag="IWD16_001197"
     CDS             complement(12933..13355)
                     /gene="rplK"
                     /locus_tag="IWD16_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP9088274.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            13698..14366
                     /gene="sdaAB"
                     /locus_tag="IWD16_001198"
     CDS             13698..14366
                     /gene="sdaAB"
                     /locus_tag="IWD16_001198"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP9088275.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            14379..15251
                     /gene="sdaAA"
                     /locus_tag="IWD16_001199"
     CDS             14379..15251
                     /gene="sdaAA"
                     /locus_tag="IWD16_001199"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP9088276.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(15342..16361)
                     /locus_tag="IWD16_001200"
     CDS             complement(15342..16361)
                     /locus_tag="IWD16_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9088277.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(16534..17421)
                     /locus_tag="IWD16_001201"
     CDS             complement(16534..17421)
                     /locus_tag="IWD16_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9088278.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(17498..18043)
                     /gene="nusG"
                     /locus_tag="IWD16_001202"
     CDS             complement(17498..18043)
                     /gene="nusG"
                     /locus_tag="IWD16_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP9088279.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(18144..18314)
                     /gene="secE"
                     /locus_tag="IWD16_001203"
     CDS             complement(18144..18314)
                     /gene="secE"
                     /locus_tag="IWD16_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP9088280.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(18338..18490)
                     /gene="rpmG"
                     /locus_tag="IWD16_001204"
     CDS             complement(18338..18490)
                     /gene="rpmG"
                     /locus_tag="IWD16_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9088281.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
BASE COUNT         5708 a         3790 c         3346 g         5766 t
ORIGIN      
        1 actcaccatg aacgtaaaaa tcttttctaa aaataattgt atgcaatgta agatggtcaa
       61 acggttttta acagaaaatc aaattgcttt tgaagaagtc aatatcgatg aacagcctga
      121 agcagtagac tggttgaaag cacaaggatt ccaaagtgtt cctgtcatta cttctgatgc
      181 agaaacagtt atcggattcc gtcctgatca gttgcgtaaa cttgccagct aacatgacta
      241 aaagaatcaa aacgtgatac atggatttga agggcttgag acagcaccag tttttgtcgc
      301 aaacttccaa atcctttttt aaagcttaaa aacaatgcaa tgatttgcaa tcaagcatca
      361 tcaaatacga aatgaaggtt gggcgtacgt atgagtctta aaaatttaca agatgtcagt
      421 tatttcaagc tgaataatga aatcaatcgg ccagtcaatg gtcaaatccc tttgaataag
      481 gataaagaag ctttagttgc cttttttgag gaaaatgtca aaccaaatac gatgactttt
      541 ctatctatta tggaaaaaat caattacttg atcgatcaag aatatataga agcagaattc
      601 attcaattgt attcaccatc atttattgaa aagctatatc aatttttgga cgagcaacat
      661 ttttctttca aatcttttat ggctgcttac aaattttatt ctcaatatgc gttgaaaacc
      721 aacgacggtt ccgcttatct ggaaacttat gaagatcgag tagccttcaa tgcgctttac
      781 tttgcgaacg gggatgaaga attagcactt gctcttgcag acgaaatgat ccatcaacga
      841 tatcagccag ctacaccttc tttcttgaat gctggacgta aacgtcgggg cgaattggtg
      901 tcttgtttct tgactcaagt cactgacgat atgaactcta tcgggcgctc aatcaactct
      961 gctttgcaat tgtcccgtat tggtggcggc gtagggatta ctttatcaaa tcttcgtgaa
     1021 gccggcgctc ctatcaaagg atatgagggt gctgcaagtg gtgtcgttcc agtcatgaaa
     1081 ttattcgaag atagtttcag ctattctaat caattaggtc agcgtcaagg agctggcgtg
     1141 gtttatttga acgttttcca tccagacatt attgctttcc tttcagcaaa aaaagaaaat
     1201 gcagatgaaa agattcgcgt aaaaactctt tctcttggcg tgatcgtacc tgataaattt
     1261 tatgaattga cacgtaaaaa cgaagatatg tatttattta gtccatatag tgtcgaaaaa
     1321 gaatatggtg tcccattctc ttatgtaaat attacagctg agtatgatca tctagttgca
     1381 aatccaaaca ttcgcaaaca aaaaatcaaa gcgcgtgatc ttgaaaatga aatctcaaaa
     1441 ttacaacaag aatctggcta tccttacgta atcaatattg atacagcgaa ccgcgagaat
     1501 ccaatcgatg gcaagatcat catgagtaat ttatgttcag agattttaca agttcaaacg
     1561 ccttctgtga ttaacggcaa acaggaatat gaagtattag gaacggatat ctcatgtaac
     1621 ttaggctcga caaatatcgt gaatttgatg caaagcccta actttggtca atctgtgcgt
     1681 acgatgactc gtgcattgac atttattaca gatgcatctg atatcgatgt cgtaccaccg
     1741 atccaaaacg gaaataagct gaatcatacg attggattag gtgccatggg cttacacact
     1801 tatcttgcaa aagaacaaat ggaatatggc tcagaggatt cattagactt tacaaatatt
     1861 tacttcatgc tgttgaatta ttggacactt gttgaaagca acaatatcgc tcgtgaaaga
     1921 aaacaagttt tccataattt tgaaaaatca gcttatgctg acggcactta tttcgaaaaa
     1981 tacgtaactg gagaattcca gccaaaatca gaaaaagtca aagcattgtt tgaaggcatt
     2041 tttatcccaa ctgctgaaga ttggaatgct ttgaaacaag cagtaatgaa agatggactg
     2101 tatcatcaaa atcgcttggc agtagcacca aatggctcaa tttcatatat caacgatacg
     2161 agtgcaagta ttcatcctat cactcgcatg atcgaagaac gccaagagaa gaaaatcggt
     2221 aaaatttatt acccagcgcc ttatctatcc aatgagacga ttccgtatta cacttctgct
     2281 tatgatatgg acatgcgtaa agtaatcgat gtttatgcaa tggcacaaca acacgtcgat
     2341 caaggcatga gtttgacttt attcatgcgt tctgaaatcc cagaaggcat ttatgaatgg
     2401 aagacaacaa cgaagcaaac gacacgtgat ttgaatattt taagacatta cgctttccac
     2461 aaaggaatca aatccattta ttacgtacgt acctttactg atgatgcaga agaaatcgga
     2521 agcaatcaat gtgaaagctg cgtgatctaa tatcatagat agattcgctt attcatattt
     2581 taattaaagg agcattctca tgtcagaaac atattatgaa gcaataaact ggaatgaaat
     2641 tgaagatatc attgataaat ccacttggga gaagctgaca gagcaatttt ggctagatac
     2701 acgtatccca ctttcaaatg acctagatga ttggcgtaca ttgtctcaat tggaaaaaga
     2761 tacagtagga catgtatttg gcggtttgac actgctagat actgtccaat ctgaaagtgg
     2821 catggatcag ttgcgtaaag atgtccgcac tccccatgaa gaagccgttt tgaacaatat
     2881 tcaattcatg gaatccgttc atgccaaaag ttattcttct atttttagta cactgaatac
     2941 gaaaaaagaa atcgaagaaa tttttgactg gacaaatacg aatccttatc tgcaaaaaaa
     3001 agctgagcgt atcaatgaaa tctacaaaca cggtacaccg ctagaaaaga aaattgccag
     3061 cgtcttttta gaaacattct tgttctattc aggtttttat actcctcttt attatttagg
     3121 aaacaacaaa ttggctaacg tagcagagat catcaaacta atcatccgag atgaatctgt
     3181 acatggaacc tacatcggtt acaaattcca attaggattc aacgagttac ctgaagcaga
     3241 acaagaaacg ttaaaagact ggatgtacaa tcttctttat gagctttacg aaaacgaaga
     3301 acgttataca gaagaattat acgatccaat cggctggaca gaagaagtca agacattcct
     3361 acgctataat gccaacaaag cattgatgaa cctagggatg gatccactct ttccagatac
     3421 tgccaatgat gtcaacccaa tcgtaatgaa cgggatttca acaggcacaa gcaaccatga
     3481 cttcttctca caagtcggaa acggctattt gttaggtcat gtagaagcga tgaaagacga
     3541 cgattatttg attggattgg aataaggatt tattttaatt caaaaatttt tcgaaaaata
     3601 aaaagagttt agcgtacaga tattttaaat gtacactgaa ctctttttta tgcttattca
     3661 cttgtttctt ccagcacttt tggactgtct aacttttccg aaacatgttc cattcttctc
     3721 aataaataga ttttccggat atttaatatt atcgtcttaa tcactgcata gacaggtatg
     3781 caaatcagca tgcctaatat tcctgcgaca cttcctgctc caatcaatac caagatgatc
     3841 gtcaaaggat ggatattcat tgatttccca aataacaacg gcttgatcaa atttccatct
     3901 aattgttgga tcactaaaat cgacaacgcc atatacaaag cttgaaatgg agagataaac
     3961 aaaccgacaa tgattgctgg taccgctcca ataaaagggc cgacatacgg gataatattc
     4021 gtgattccgc aaatgaccgc aagcaacaag ctataaggct gatcgaatat tctcattgcc
     4081 aaaaagctca ttacaccaat aatcaaagca tctaagatcg tactgctgat ataggcagat
     4141 aatgtttcgt ttaattcttt taccgtttgg cgtaactctt tacgaatcgc tttcggaaag
     4201 aagtgagaca acgcatcaat aaatttgtgc ccatccttga acataaatag caaaataaat
     4261 ggaacggtaa ataacaaaat aaaaaattgc atcataaatg aaatgatttt tgagacactt
     4321 gcagataaac tgactaatat gatattgaac aaattactaa gagataaatt tgctgatgct
     4381 aattgttctt ccaaattgaa atttttaaac tcttcccgct gcgccaattc atttaaccat
     4441 ttgcttgttt catctgcata cattggcaag ctctgggtca attgaattgt ctgttccatt
     4501 aactgaggca ctacattcat gacagttaaa acaactaaaa tgattacgac caaaaacgtc
     4561 aataaaaaac cgaacactct cgggatccga tgcttttcga gaaaaacaac gattggatcg
     4621 aacatataat agataaaacc agcaacaagc aatggtaaaa aaactgccga aagcattttc
     4681 aatattggac taaaaatatt cggcatttgt aaaataaagt acacgccgat gatcgtgagt
     4741 actagctcca ctgtccagaa aaataatttt gattgtttaa aacgtgattg catacatctc
     4801 ctccaaggtc ctttgatact tctattctat catttaacaa agagttacgc gctgagattt
     4861 tcatttgctt aaaaaaaaca aaaaaaataa acatatttta atgagtagac gagtacttaa
     4921 acaaaaacac ggtttggagt agtgacaaaa attatactgc agtccctttt tccaaataaa
     4981 cggtgttcaa aagcttgcat cttcgtaata agcccagcca aacgaaaaat ataaggaact
     5041 atttttattt ggctgggctt attacttgat gtaggacatc tattttacaa cctcagatca
     5101 tttattgcgc aatactgact aaatgcaact ttgtgatatt tttgtgtacg gacaactcct
     5161 cgatcacatc ggcataagtg agcccttttg gaagatcaat cgtatagata tttttgtaca
     5221 ctcggtagtc atcaacgaat tttacgtcaa aattcacatc ttcgattacg atatttttct
     5281 cttcaaaata atcggtaaga aaatctttgg tttcttttct atggataaat tggatttcta
     5341 actttttagt cgtaggtaca tggatcacgc gtttcactac tgtgagtgca atagcaatcc
     5401 cgataaaact gatgatcgca atggaatagt aacccatgcc gatggcgatc cctaatccag
     5461 caattgccca taaagaagcg gcagtcgtaa gtcccgtcac cgattgtttc gtgacgatga
     5521 tcgttcccgc gcctaagaaa ccgacaccac tcaccacttg agcaatcagc cttgcttcat
     5581 ctgaacgaat cgttccagca agctgtggat actctaaagc atctcttaat gcaccactag
     5641 cgatctcaac ttggatcaat gcaatgatac atgctcccat acaaactaaa atatgcgtac
     5701 gcatacctgc tggacgattt ttatattgtc gttcaaaacc gattgctccg ccaaatagca
     5761 tagcgatccc taaacgtaaa ataatctctg gaattgtcaa taccatatca ttcatctaaa
     5821 caccctccag tccaatatat catgtctgaa aggagcagac aaaaaaagtg caatttttac
     5881 ctcttacgaa aaaaggactg tgacaaaatt tttgtcatag tcctttttat gaataaacgg
     5941 tgttcaatca gctgaccctc ggtattactc aacaaacggc aaaatatggg gagctatttt
     6001 tcctttgtct tctctaatac tcaggtcaaa acgctgattt cacaacctct ttagcctact
     6061 attgaaaatt acaacttact tgctgcttct tcatcgatga tcacaacaac attgtcgtga
     6121 ttttgcaata cacttgctgg caaatgattc gttacaggtc cttcaatcat ccctttgatt
     6181 gcatcagctt tgctttcgcc aaatgccatc aagacgattt ctttactttt cataattgaa
     6241 ccgattccca ttgaaacagc ttttgttggt acatcttctg ctttatcgaa gaatcgtttg
     6301 tttgcattga ttgttgattc tgttaagtca actactgaag tcgttccgtc aaatgaagca
     6361 ccaggttcat tgaaaccgat atgtccattt tgcccgattc ctaagatttg gatatcgatt
     6421 ggatgtgcat cgatgatttg atcatacttt ttgcatgctg cttccagatc ttctgctttt
     6481 ccatctggta caaacgtttc tttgaatggt tttttatcaa ataattggac atccatgaaa
     6541 tgacggtagc tttgttcatc tgaaccgcct agtccaacgt actcatctaa gtttacagaa
     6601 gtcatttcac tgaaatccaa atcgcttgaa gtcatttctt tatataaagt aactggtgtg
     6661 cttcctgttg ccaatcctaa tacttttgca cctttgttca taccttcttt gatgatttca
     6721 aatgcttttt ttccgccttc ttcagcgttt tttacacgaa tgatttccat agtgattgct
     6781 cctttatatt ttggtatagc ccaattatag cattcttttt ttctttttac aataattagt
     6841 atagaccaaa ttgcattttt ttcattttct tgacaataaa taggaatccg tgttaggttt
     6901 cataagatgg ttcaatcaaa caatccatgt tgcatgaaaa ccaagcaagt gattcactgt
     6961 ggacgcttta tgtctgcagt ttttttatga aaaaggagtt attatgagta tactgacatt
     7021 aaacaacatt acccagcaat ttggcgacaa aatcctctat gaaggtgtcg atctgcaatt
     7081 aaatgctggc gaacatcttg ggctgattgg acaaaacgga gccggtaaaa gtacattgat
     7141 taaaatcatc actggtgaga tcttaccaga tgacggacag atccattggc aaaaaaatat
     7201 tcacaaaggt tacctcgatc aatatgtgga agtcgatgaa acattaacaa tcgaagaatt
     7261 tctaaaaact gcgtacgcaa aagaatacga aaaggaagaa aaaattgctt ttctttacca
     7321 aacatatagc gagaccttcg atgattcctt gctggaaaaa gctgggaaac tccaaacgga
     7381 attagaccaa ggagtgtttt atcaaatcga caccttagtt gctgagatga gcagcgggtt
     7441 ggggatcgat gtcttaggtt tagattcact gttgaaaaat ctaagtggtg gacaacgttc
     7501 aaaggtgatt ttagcaaaac tacttttaga aaacccagat gttctgatct tagatgaacc
     7561 aaccaatcac ttagatgatc agcatatcca atggctgacg cagtttcttc aggattttga
     7621 cggtgcgtat atcgtgattt cccatgacca agaatttctt gatcagatca ccacccatat
     7681 cgcagacatc gagtttggaa agattactaa gtatactggc catctaaaac aagcattgaa
     7741 acaaaaggaa caaaatcgcg aaagctattt gcgccaatat catgctcaac aaaaacaaat
     7801 cgaaaagacc gaagcttata ttcgaaaata caaagccggt tcccgatcaa cgatggcaaa
     7861 aagccgacaa aaacaattag ataaaatcga acggctgacg cctccagctt cagcaagtaa
     7921 agctgtcttt gattttccat tctcgcctat tgtcacgaca ttagcagtgg aaacaactga
     7981 tttagtcatc ggatatgatc gaccgctact tgcacccatc aatttgacca tccgctttgg
     8041 tgaaaatatt gccattcgag gcttcaacgg gattggaaaa tctactttat taaaaacact
     8101 gatcggtgag attgaaaaaa taagtggcga tttccacttc ccagataaca caaaaatcaa
     8161 ttacttttca caagatctgt actgggaaaa tccattagaa acaccactac aatatttaag
     8221 cagtcaattt ccaaaagcga ctatcaaaga attacgtcgc caactagcta aagcagggtt
     8281 agttaatcaa ttagcaagtg aacctctcac tacattaagt ggtggggaac aaactaaggt
     8341 gaaacttgca cagttgacaa tgaaccaagg taatttactg attctcgatg aaccgacgaa
     8401 ccatatcgat caagaaacga aagaaagctt acaagacggg atacagaaat ttccaggaac
     8461 agtaatcatc gtctctcatg aacaagaatt ttatcaagat cttgtgcaac gagtcattga
     8521 gattgaacca aaataaacgt ttgattgaat agttgaaaaa aaggagctgt gacaaaagtt
     8581 ttgtcacaac tccttcttct gaataaatgg tggccaatta gctgaccttc ggtattagct
     8641 caaaaaacgg caaaatatga ggagctattt ttatttggct attcttatta cttgatgcag
     8701 aacagctttt cacaacctct tttcgtctat tttttatttt cgagatttct ctttccttta
     8761 aaaacaactg tttgaaagaa gaacgcacat ccttgaaaaa caagcccgct aaccgttaca
     8821 cctaaccagc catactgctg ccacattgtc gtccctagca atgaaccagc cgcacctcca
     8881 ataaagtaca taaacataaa tacagtatta tttcggttgc ttgctgcttc acctagccct
     8941 tgtactcgtg tttgattggc gacttgacca aattgattcc cgacgtctaa taaaataata
     9001 gctaaaagca aaatggccaa atggcttccg ccaaccaata aagcaataaa gcttattgtt
     9061 tgcataacaa gtcccatata cacgattttt ctttcagaat aagaatctgc cagtcgtccc
     9121 acgataggtg cagcaactgc tccagacaaa ccaaaaatcg ctaatatccc tgcttcgaat
     9181 gttccccaat tgtaggcggg actgctgata taaaaaatca acgtagacca aaagatcgaa
     9241 aatgttccaa acatgaaaaa tccactgact gccgcttctc ttaatagacg ttgagaagca
     9301 actaaatgcg gcaaactttt aagtgaatca aggtatgaaa cagctgtact actttgctgg
     9361 acagaacgcg gcattttcca gtaaagcaga agtgtaagaa ttccgactgc acataaagca
     9421 aataaataga tcatccgcca agaaaaaaga ctggcaacaa taccagatac cgtccgagaa
     9481 agcaaaatcc ccacgagtaa accgctaagc aatgtaccca tgaccttccc tcttgctttt
     9541 ggtcccgcta gtaccgcacc gtaaggaatg atgatttgcg gcacgatcga aagtaatcct
     9601 attaaaaaag aagcgcatgc aaataacgta aacgaaggcg caaaaaaagc ggccaacaga
     9661 gagacggcag ataacgctgc cattcgaatg atcaattttg gacgatccac cacgtcccct
     9721 aaaggaacta aaaacagtaa ccctaacgca tagcctaatt gggtcaacat cgtcaatatc
     9781 ccgactgcac ttgtgctaac agataaacta gctgctactt ttgttccaat cggctggatg
     9841 taatacatat tggcaacaac aacgccacaa gtgattgata atagtaaaat aagcgaagtt
     9901 gttaaaggtt ttttttcatt actcttcatt gatttccccc atttcctatc ttagtttaca
     9961 acttatggaa aagattggaa atagaaaaat acaactattc gccacactta ttatttgtgc
    10021 ttttgttttc cttgtttttt cttgcgatct ttataaatga agacaactaa ccctactagc
    10081 acagtaacac ttgcaaccaa cgtgactgca gttttgttat atccgtgaaa cagagcttct
    10141 ccaccacacg catacaacac agacgaaggg atgactccaa tcacgatcga caatatgatt
    10201 tgctgtagag gcaatttcat cgcatctgca gcaaaattga ccatagaaga tggaataacc
    10261 ggtatcatat agcctaacat tacacctatt tttggatgtt tcatttgtcg gattgcttgc
    10321 acccaatgat tcgtttcggt tttcttatct aaaaatttta agcgatgcat aaggaaaatt
    10381 gcagtgatgt taccaagagc attacccaaa atgttgatga tcgcacctgt cagtggtcca
    10441 aagctcaaac cagcaatgac tccaactact gaagtaggaa tacctggaac agcacacatc
    10501 accgctgtca atacgatcaa gagtatcgat gcaaaagtcc catgcgaacg aatatgtgcg
    10561 agtagctctg ccttactcgc tctcggattt aaaaataatt ggatgtctgt ctgatattct
    10621 aaatacaacc gatagcccac taccagtatc agaatgattc ccaacgagat caatatcaat
    10681 cggattattt tctttttcat atttcctcct gaaaaaaatc tatattcatt aaataatagt
    10741 ttaacataaa aacaaaaaag caccgaaatc caacatagga tctcagtgct tttaaatagt
    10801 aaaaattatt taacagttac agatgcgcca acttcttcaa gtttagcttt caattcttca
    10861 gcttcttctt tagatacgcc ttctttaact ggtgcaggag cgccatcaac tacagcttta
    10921 gcttctttca agcctaaacc agttgcttca cgaactgctt tgataacttt aactttttgg
    10981 tctccagctg cagttaattc tacagtgaat tctgtttgtt cttcagcagc agcagcgcca
    11041 ccagcagcag ctacagctac aggagcagca gcagatacgc caaattcttc ttcaatagct
    11101 ttaactaggt cgttaagttc taggatagtt gcttctttta actcagcaac gatgttttca
    11161 atgttcaatg ccattataat ttcctccgtt taattgttct tttttattta atgctttatg
    11221 cggcaattaa gctgcatctc catcttcttt gtctgccact gctttgacag cgtaagccac
    11281 gttgcggact ggcgcttgta gtacagatag caacatagat agaagtcctt cgcgatttgg
    11341 caattttgcc aatgctgtga tttcttccaa tgaagagact ttaccttcga tgataccgcc
    11401 tttgatttct aatgcttttg cttctttagc aaattcgtcc atgattttcg ctggtgcgac
    11461 tacatcttcg ttactgaatg caacagctgt aggtccagtg aatacttcgt ccatgccttc
    11521 aaggccagct tttttagctg cacgtgaaag aatagaattt ttgataactt tcatttctac
    11581 gttagcatca cgaagttgtt tacgtaaacg tgttacttca tcaactgtta atccgcggta
    11641 gtcaacgact actactgatg cagctgctgt aaatttttcg cttacttcat caacgattgc
    11701 tgcttttttt gcgataattg cttcactcat ttttgagttt cacctcctga attttgatgg
    11761 aagagttttt tgcactgaaa aggcaagtca cttttatagt tactttgctc aaccagtatt
    11821 attcatcacc tattcacttc gttcatagtg attctactcg ctttgctcga aaggaaactc
    11881 ctttcgcttt gctcaaccag tattgttcat catctcttca cttcgttcat agtgattcac
    11941 aataaaaaac tctatgccac cgcagacata gagggactat tgaatatatt caataaacgt
    12001 cctcggtagg aaattaaggc ttgatgccac ctactgtctt cggtacaagt taattattaa
    12061 cctcaactag cttagcatgc taaactagtt gagtcaagaa ttatttttca gttggatcaa
    12121 tcaacattct aatatttaat tagaaagaag cttggtcaac atggatacca ggtccaaatg
    12181 ttgttgtgat cgttacattt ttcatgtatt gacctttagc tgctgatggt ttagctttta
    12241 gcaatacatc atggattgct gcaaagtttt caactaattt ttcatcgtca aatgaaactt
    12301 ttccgattgg tacatggata tttccagctt tgtccacacg gtaagttact ttaccagctt
    12361 tcacttcgtt tactgctttt gttacgtcca ttgtaactgt accagtttta gggtttggca
    12421 ttaagccttt tggtcctaat actcgtccta gacgacctac agtagccatc atatcaggtg
    12481 ttgcaacaac tacatcaaag tcaaaccatc cgttttggat tttttgaacc atgtcgtcgt
    12541 cacctacgaa atctgcacca gctgcttcag cttcttttgc tttgtctcct ttagcaaaaa
    12601 ctaaaacagt ttgtgtttta ccagttccgt ttggtaatac aacagcacca cggatttgtt
    12661 ggtctgcttt tttagggtct acgttcaaac ggtatgcaac ttcaactgtt gcatcaaatt
    12721 ttgcaatgtt tgtttctttt gctaaagcta ctgcttcggc aacaggataa actttacttg
    12781 aatcaacttt ttttaatgca tcttgcattt ttttgctctt tttagccatt ttggttcctc
    12841 cttgattgtg gttataacgg ttgtacctcc cacgtgttct atatctttca atatagagcc
    12901 aactgagaag ctgctgtctt agggtgtata gattattcta cagtgatccc catgcttcgt
    12961 gcagttcctt caaccatgcg cattgctgct tcaacgttag ctgcgtttag gtcttccatt
    13021 tttaattcag cgatttctct tacttgatcg ctagaaactt tcgcaacttt atttttgttt
    13081 ggttcgcctg aacctttttc gattttagct gcttttttca atagaacagc agctggcggt
    13141 gtttttgtaa cgaatgtgaa tgaacggtct tcgtatacag aaataacaac cggaatgatc
    13201 aaacctgctt gatcagctgt acgagcgttg aattcttttg tgaatcccat gatattgata
    13261 cccgcttgac ctagtgcagg acctactggt ggagctggag ttgctttacc tgcaggaatt
    13321 tgcaatttaa cgattttttc tactttcttt gccacgagac atacctcctt aagtccgtga
    13381 tgtggttaat tggagatgca tatttctcct cccacgtttt atctgatctg aaaaaatcca
    13441 gacaaataat gtgcaaaaca atgcacactg gtacagtata gcaaatttat ttgttttttc
    13501 aagatttttt tctgctattt ttacattttg cttgaataaa cgctaaattt attctaaaaa
    13561 aacaacaatt tttcgttctc atttcttctt ttatgttaca cttgcgctag catggttcaa
    13621 cagatgaaag caaacaataa cacggttttt tagacccttg tacaaagatt cctacataac
    13681 aaaatgaggt ggagaaaatg aaagatttaa ggtatagaag tgtctttgat attattggac
    13741 ctgtgatgat tggtccaagt agctctcaca ctgccggagc tgctagaatc gggaaaatca
    13801 tacgtagtat atttggcgaa cagcctgatt ctgtagatat ttatctatat gaatcatttg
    13861 caaaaaccta tcgggggcac ggaacagata ttgcattagt tggcggcctg ttagggatgg
    13921 aacccgatga tgaacgttta gctgattctt tgaaattggc atatgaagcc ggtatggaag
    13981 tcttgtttgt tcctaagagt gaaaaagccg aacatccaaa ctctgtaaag atgcttctaa
    14041 aaaaaggaac acgaaagctt tcagtcactg gcatttcgat cggcggtggc aatatccaga
    14101 tttctgaatt gaatggattc aaaatttctt taagcatggg tactcctacc ttgatcgttg
    14161 tccaccaaga tgttccaggt atgattgcta aagtgactag tatactttct gagaccaata
    14221 cgaatatcgg cactatgaca gtgacacggg aatcaaaagg tgaaaatgcc attatgataa
    14281 ttgaagtaga tgatcctcaa gtagaagaaa cggtaaaaag actcaaacag ctgccaaata
    14341 tcgacagcgt aaattatttc gattaaggag ctaaaaaaat gtttttatcg attgaagaat
    14401 tagtagatca agcaaaagct tatccaagtg tagcagaatt gatgattgca gttgaaatgg
    14461 aaatgagtgg ccgttcccgt agtcacatca tcgagatcat ggaaaaaaac ctctcagtaa
    14521 tggaacaatc aatcaacgag ggaaaagaag gtgttacctc cgtcacagga attaccggtg
    14581 gtgatgcaaa aaaattagat gattatttaa atgaggcaga ctttttaagt ggagaaacaa
    14641 tcttaaaagc tgtacgcaat gctgtagctg ttaatgaagt caatgcaaaa atggggttga
    14701 tttgtgctac accgactgcc ggaagcgcgg gtgttgttcc tggtgtgctg atggctgtcc
    14761 gagatcggct tgatttgaca catgaacagc aattggaatt cttatttacc gctggcgctt
    14821 tcgggttagt catcgcaaac aacgcttcta tcagtggtgc agaaggggga tgtcaagcag
    14881 aagttggttc tgcaagtgca atggcagcag cagctttggt ttgtgcaaaa aatgggactc
    14941 ctcatcaagc agcacaagca gtagccatta cattgaaaaa tatgatggga ctgatttgcg
    15001 atcccgttgc aggcttagtt gaagtcccgt gtgtcaaacg taatgcttta ggctcttctc
    15061 aagcttttat ctctgctgat atggcattag cgggtatcga aagtgtcatt ccgccggatg
    15121 aagtcgttgc tgcaatgtac caagtcggac gtcaaatgcc gtcgattttc aaagaaacag
    15181 cagaaggtgg cttagctgtt acgccaactg ctaaacgact tgcggaagag attttaaata
    15241 agagggcgtg ataaagctcc ttaatttcga gtattaagag attagttatc ttcataacaa
    15301 aaaacgaaga gaggactaat tgcccttctc ttcgtttttt attaatccat caatttcgta
    15361 ttttttactt catcgatcgt ttttgttcct gcaagttgca tcgtgatcga caattctttg
    15421 ttcaggtgtt caaaaacaga tgttactcct tctgccccac ctagattcaa cccataaatc
    15481 actggtcggc caatcgcaac taagtcagca ccacttgcca gagctttgaa gacatgctct
    15541 cctcgacgga caccagagtc gaaaatcact ggtacacgtt tgttgactac ttcggcaatt
    15601 tttggcaata cttcaaagga cgcaggacct ccatctagtt gacgtccacc atgattggat
    15661 acccagatac catctgcccc tgcagagata gcgattactg catcttctgg cgattgtatt
    15721 ccttttacga taactggaag atgggtgatt tctttgattg ttttgatgtc atctggtgtc
    15781 aagccttgtt ttgcagctgc ataaatttct gcgattcctt ttccttcgcc attaccactt
    15841 gcactttgtt cgctatacgc agctaagtta ggcattggta atggaaattg gaattgattg
    15901 atgacgtctt cttcacggta accaccgagc gttgaatctg ccgttaaaat gatagcttta
    15961 gcccctgctt ctactgcttt gttcaaaatg aactcattga atctgtcgtc tttactcata
    16021 tataattgga aaaactgcgg tgcgcctggc gctgcatcgg ctgcgtcttt gattgtcgta
    16081 tttgcataag tgctgatgga aaagattgat ccagcagcag ctactccttt agctgtatca
    16141 gcttcccctt tttcatgtgc caatccttgg gccgctgatg gcgcttggat aatcggtgta
    16201 tctaattcaa ttccgaaaac agatgtatgc aaatcggctg agtcgattcc gcgtaatatc
    16261 cgtggcatga ttttctttgt attaaaagaa gtcgtatttt ctttcatcgt ccactcatct
    16321 tcagaaccac cgcgaatata gccaaaagct cctgcttcca tgcgctcttt tacacgacct
    16381 tctagttagg caatgtttac aatatcgatt ggatgttctt cattacttgt atgataagtc
    16441 atagtagatc cttctttcat cttcttgatt acatataaat catactaaac ttacaaaaag
    16501 taagcaaaca tttactctca gatactttta tcgctattct ttccaaataa atcgacttac
    16561 aagctgatct acttcttggt tttcatgaag catactatga gaggcatttt cacctgtcac
    16621 tactttttcc tctatttcat ttccgcgttg cttcaacaac gcatagaccg ctaatgcact
    16681 gtttaacggt actgttccat cactcaagtc tgtttcatct agttgaccag caatcaagaa
    16741 aaatcgagtg gttataggaa tattaccgat catttgctgg taatcttgat agcggttaga
    16801 aacgacagca ggcccctttg ctaactcatc tgtcagagac tgcgcactat catcgacaaa
    16861 atcattaaaa ggcgcgccaa ttgctgttaa tgtctgaact tgtggcaatg acgtatcctg
    16921 tccatacgtt cctaaatagc gtagtgcact aactcctccc atggaatgcc ccactatgtt
    16981 gactttatct atgccgtagt tttctttcag atacaaaaga caagttttga tccattctgc
    17041 ttgattccac tcgttatttt tattatcttc aaacaatact tggaccatcg gattgtctgc
    17101 ttttccagac aaatctcctg ttgcttgtat ttgtccatct gttcctactg ttaaaaccaa
    17161 ttcttttact gcttgctgat ttttttccaa acgccgcaac atccctttaa aggaattttc
    17221 tgtcccgctg tagccgtgga aaaaaagtgt tggaatagtg gttttttcta tcactttact
    17281 ggattctgcc gttttttctt tatcagaaac ttcagtagta tttatcttcg tcgtctggca
    17341 accacttaac gcaaacaaac tgataaggac taaagctgct gcagttgcac gtttgcataa
    17401 atttttgatt ggttctgtca tacacctctt cctttccata tagaaaaaag aaagacttga
    17461 aaggccaatc atatgatcga catttcaagt ctctttatta atcgatttta tctacttgat
    17521 cgaaatcaag ttctgtactg gtttcacgtc caaacatatc gatgttgact ttcaattttt
    17581 ctttttctcc gtcaacttct gttacttgtc cttctaatcc agcaaatgcg ccttcgatga
    17641 ttttcacgac ttcgccaact tctacttcta gatcattttg tcgcaggctc ataccgatag
    17701 aacgcaagat atgattgact tcttctggaa gtaatggtgc tggtttgctt ccggctccgt
    17761 gtgaaccaac gaatcctgtc acaccaggtg tgttgcggac aacataccat gaagcgtctg
    17821 tcatgatcat ttcaacaagt acgtatcctg ggaatgtttt atgaacgatt tctttttctt
    17881 tcccattttt gacttcagtt tctttttctt caggaacaac gacacggaaa ataaagtcgg
    17941 ccattttcat actttgcgca cgagattcga tatttgcttt tactttgttt tcatatcctg
    18001 aatatgtatg caagacatac cagttttttt caaaagattc cattgaaggc tcctcatttc
    18061 attgtaaaat aaaaaaacct cagtacaccc gaagtttttc tttcgcttca ttatagcact
    18121 aaaagtacgt tgtggctagc gagttatttt aaaatccagc caaataagct ttggatggct
    18181 gtatccatta cgtaaaacat caatccgaaa ataatagaag tttcgatgac tactaacgta
    18241 tctttacgga gttgttttct gcttggccaa gttacttgtt tgatttcatc ttttacgctt
    18301 tttagaaatt tcatgagaca acctcccttg ctactgttta tttcgtttct ttatgtaatg
    18361 tatattgatt acaatattta caaaacttat tgatttccaa acgttcgccg ctttttcctt
    18421 cactgactga ttttgtgtag ttgcgagacc cacaaacgga acaagcgagt gctgcttttt
    18481 tctttgccat ttacttctgc ccccttgctg agaatcagac ttatacttca taacagtatc
    18541 acgtaacttt ttacttgtca atttgtgaaa taaactatct attgttttgg ataaaattat
    18601 gatatgatta
//
LOCUS       DACDMM010000046        18250 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDMM010000046 DACDMM010000000
VERSION     DACDMM010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.046"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            79..1764
                     /locus_tag="IWD16_001205"
     CDS             79..1764
                     /locus_tag="IWD16_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP9088282.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            1778..3061
                     /locus_tag="IWD16_001206"
     CDS             1778..3061
                     /locus_tag="IWD16_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9088283.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            3131..4000
                     /locus_tag="IWD16_001207"
     CDS             3131..4000
                     /locus_tag="IWD16_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9088284.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            3997..4833
                     /locus_tag="IWD16_001208"
     CDS             3997..4833
                     /locus_tag="IWD16_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9088285.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            4859..5926
                     /locus_tag="IWD16_001209"
     CDS             4859..5926
                     /locus_tag="IWD16_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP9088286.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            5923..6750
                     /locus_tag="IWD16_001210"
     CDS             5923..6750
                     /locus_tag="IWD16_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9088287.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            6795..7820
                     /locus_tag="IWD16_001211"
     CDS             6795..7820
                     /locus_tag="IWD16_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9088288.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            7817..10000
                     /locus_tag="IWD16_001212"
     CDS             7817..10000
                     /locus_tag="IWD16_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9088289.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            10000..11130
                     /locus_tag="IWD16_001213"
     CDS             10000..11130
                     /locus_tag="IWD16_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9088290.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            11127..11804
                     /gene="pgmB"
                     /locus_tag="IWD16_001214"
     CDS             11127..11804
                     /gene="pgmB"
                     /locus_tag="IWD16_001214"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9088291.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            11888..12415
                     /gene="msrA"
                     /locus_tag="IWD16_001215"
     CDS             11888..12415
                     /gene="msrA"
                     /locus_tag="IWD16_001215"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9088292.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12616..13302)
                     /locus_tag="IWD16_001216"
     CDS             complement(12616..13302)
                     /locus_tag="IWD16_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP9088293.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            13382..14920
                     /locus_tag="IWD16_001217"
     CDS             13382..14920
                     /locus_tag="IWD16_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP9088294.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15040..15507
                     /locus_tag="IWD16_001218"
     CDS             15040..15507
                     /locus_tag="IWD16_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9088295.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            16026..16469
                     /gene="rplM"
                     /locus_tag="IWD16_001219"
     CDS             16026..16469
                     /gene="rplM"
                     /locus_tag="IWD16_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP9088296.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            16485..16877
                     /gene="rpsI"
                     /locus_tag="IWD16_001220"
     CDS             16485..16877
                     /gene="rpsI"
                     /locus_tag="IWD16_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9088297.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16965..18189)
                     /locus_tag="IWD16_001221"
                     /pseudo
     CDS             complement(16965..18189)
                     /locus_tag="IWD16_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         5909 a         3333 c         3874 g         5134 t
ORIGIN      
        1 aaatgctatt gacagtaagc gttttccata atatgatcaa atcgttggga acgctcccaa
       61 aaaagaaagg ggaatccgat gtatacaaaa cttttacaag cgatttatcg cgactcagat
      121 ttgtctgaga aaaccgcctc tatgattgaa caaaaagtca gtgtttatca gcagaagaaa
      181 ggccaggcag gcaaactgca ttgggacgaa gaagatattt tcttgattac ttatggggat
      241 caattttttg aagagcagca accaactttg cgtacattta agaaatttta tcaaaagtat
      301 ttggcagata catttagagt tgtacatttc ttgccatttt tcccatatac atctgatgat
      361 ggtttctccg ttgtaaatta tcaggcaatc aacgaccgtt tgggcacatg ggaagatata
      421 gaagaaatga gaagcgaaac acggttaatg ttcgactttg tctgcaatca tatgtctgcc
      481 gaaagtactt ggtttcagga atatttaaac ttgaatgata cgtacaaaga tttttttatt
      541 gatttgccac tggatacaga tacaagcagc gtgactagac caaggaccag cccgttactt
      601 acaacgttca ttgacaaaaa cgggaaagaa aaacacgtat ggacgacttt tagcgaagat
      661 caagtagact tgaattttaa aaatccagaa gttttattga aaatgatcga tattcttttg
      721 ttttacttag aaaaaggcgc ttcatttatt cgcttggacg ctgtgggatt tttatggaaa
      781 gaactaggaa cttcttgtat acacctgcca gaaacgcata aaatcatcca attattccgt
      841 gcaatcgctg atgaagcagc tccaggaacg gtattgatca ctgagacaaa tgtgccacac
      901 aaagataata tcagttattt tgggaatggc gagaatgaag cacagatggt ctatcagttt
      961 tcgctaccac ctttgaccct tcaagcgatt cgttcgggta atacaagtaa gttgaaagaa
     1021 tggttaaaaa ccatcgattt cgcatcgaaa aaaacaactt tttttaattt tttagcctcc
     1081 catgacggta tcggattgaa tccggtacga ggtattctgc cggaaaaaac gatccttgaa
     1141 ttagtggaac agctgaagaa agagggtgcc cgagtaaact acaagaaaaa cccagatgga
     1201 acgaccagtc catatgagat aaacgctacg tatctagatg cgttaagttc tccaacagat
     1261 acgacggaat ggagaagcaa acgcttttta gctgctcatt catttttggt aacgttacca
     1321 ggcgtgccgg caatttatat tcaaagtgtg ctgggaagtc gaaatgatta caaaggcgta
     1381 gaaaaaacag gagagaatcg aaaaatcaat cggcaaaaat ttttgttagc agatattgag
     1441 aaagagttgc agacagatcc attaagaaca gcagtatatg gtgaattgac ccagatgcta
     1501 tctattcgaa aaaaagaaaa agccttccat ccgaatagtt tgatttactt actggactca
     1561 gcagattctt ttatcagttt tattcgcgaa gcgaaggaag agaaagtctt agttatccat
     1621 aatttttcca atcaatcagc aggttatctt ttaccaaaag gagattacct agacctgttt
     1681 tctggacaaa ttatccattg cagtcaagaa cgaaagttga tattccagcc gtatgaatat
     1741 atgtggctga aaataataaa ataagaggtg gaaaaaaatg aaaaaaatta ttgttactac
     1801 ctgtctgcta gctactggat ttcttcttac tgcttgtggt gaaagtcaaa gctctaacga
     1861 atcacaacag acaacgattc aattcatgca ctcttctgtc gaacaggaac gattaagtgt
     1921 gatcaatgaa ctggttgctg attttgaaaa agaaaatcca gacatcaaaa tcaagcaagt
     1981 gccggtagaa gaatcagcgt tcaatacaaa aatcgttacg ttagctcgtt ccggtaaatt
     2041 accagaagta atggaagtca gtcaggattt tgctaaagtg atggataaag atgaactgat
     2101 tgatcagacg gcagtccaaa acgttatcga ggaagctgga gaagataatt attatgacgg
     2161 agccaaaaac cttgttcgtt cagaagatgg aaaaagctat attgctgcac caattagcgg
     2221 ctgggttcaa ggtatttggt atgacaaaga aaaattatca gaagctggtt tttcagaacc
     2281 agaaaattgg gatgatattt taaaaattgc tcaacatttc acagataaag agaataaaca
     2341 atatggaatt gctatgccta ctgctgaagg aacaatgagt gagcaagcgt tctctcaatt
     2401 tgctttgtca aatggagcaa atgttttgga tgatgaaggc gaggtaacga tcgatacgga
     2461 aaagatgaga gaagcattgt cgttttatca aaacttatcc caatacacga tgccaggctc
     2521 taatgatgtg acagaaatca aagatgcttt catgaatgga acagcaccaa tggcaatcta
     2581 ttcaacttac attttgcctt ctgtttatga agcggggaac tcagaaaata tcggttttgc
     2641 tatcccaaca caaaaagatc aagctgtcta tggcacagta tctggattga cgatttcttc
     2701 tggtctagac gagaaacaaa aagaggctgc tgaagcattc acagctttct tatctcaacc
     2761 gaaaaacatg gaaaagtggg tcttgatgtc tcctggtggt gctcaaccag tgaataaaca
     2821 agtagtcgaa ctggatggtt acaaagaaaa tgaagtcatc aagtcttttg gcgagctgcc
     2881 atctgagatt gctagtgctt ttgatgaagt acaagtcttt ggactagtcg gggaaaagaa
     2941 tttcacgaaa atgggagata tcacaagctc aggtgtgatc ggaaaagcag tgaaccaagt
     3001 aactgtcgga aacgaagatg tcgatcaagc tttagctgac gcacaaaaaa atacgaaata
     3061 acaagaagtt ctgccaatgg tgttcgaaaa aagtagatag ctattggcag attatcaggg
     3121 ggaggtttca atgaatcgtc caaggaaaca ttctgactta aaattagcca tgggactttt
     3181 agcaccaagc atgcttttat tagctgtttt agtgctgatt ccaatgcttt ccaatattca
     3241 gatcagtttt ttcgatatgc cgctgaatcc taactcagac gccgttttta tcggattaag
     3301 caattatctt gaaattttaa gtgatagtga attttatcgt tctttaggtt taacagttgc
     3361 ttacacattc ttagttgtag caggcagtac agctttagga ttagccgtag ctattttgct
     3421 taaccgcaag ttcagattta ggaaaacggt gcgttcactg attttattaa gttatgttac
     3481 gccatctatt tctttgattt ttgcttggaa atatatgttc aataatagtt atggaatcgt
     3541 caatgatctg ttagtagata ggttgcagct tttcaacgaa gcaccgctgt ggttcgacca
     3601 gccagttgct agcttcttta tggtcgtttt gtttgctatc tggagatact atccgtatgc
     3661 gtttatttca tttttggcta ttttgcaaac agtcgacaaa acgctttatg aagcagctga
     3721 aatggacggt gcaaatgctt ggcagaagtt caagatcgtg acactgccgg caatcaaacc
     3781 agtactagca actgtgattg ttttacgatc tatctggatg ttctatatgt ttacagatgt
     3841 ctatctactg acgaataaag tcaatatctt aggcgtttat ttgtatcaga cagcctttgc
     3901 gttcaatgat cttggaaaag cagcagctat atcgattcta ttgttcatca ttattgcagc
     3961 ggtcgtcttg ctctcacgaa agaaggtgga cttgaaatga aaaataaaaa gagcaggaaa
     4021 atcttatttt acgcgacgat tttgttgcta ttagctgtca atttgtttcc atttttgatc
     4081 atgctgatga catcattcaa ggattcacaa gaagcaattg caacgacaca aactattttt
     4141 ccgaaagaat ggacttttga acattatcag gatattttca atgctgagat ttttccattc
     4201 atcatgtatt tcaaaaatag tttagtgatc tccttgatag cctccgtaac tgcagtagtg
     4261 attgggatca gcggggcata cgctttgtca aaactacgct tcaaagctcg ctcgaaaatc
     4321 aatgctagct tttacaccgt ttatatgttt tccggtattt tgatcgttgt tccattattt
     4381 aaaatcattt ccggtctagg tttgtatgat accaatacag cactgattat cacgatggtc
     4441 gttcaaacac ttcctactgc gatattcatg ctaaaaagtt attttgacac gattccgact
     4501 gatattgaag aagcagcaat gatcgatggt cttagccgga tccagatcat tattcggatc
     4561 attataccat tggctatttc tggaatcatt tctgtcttcg tttattgctt tatggtttcg
     4621 tggaatgatt atttatttgc ttcgattttc ctgtcgacct ccgaaaaatt tactttacca
     4681 atcggtttga acacattatt cagtacacca gactacattt ggggacgaat gatggccgct
     4741 tcattggtga ccgctttacc agtcatcatt atgtatgcgt tatctgaacg atttattaaa
     4801 ggtgatttag tcgaaggcgg cgtaaaagga taaaaagaaa agaaagagga aagaaaagat
     4861 gaaaaaatta gttgcattag aaccaagaat agccggattc aaagattacg aagaccgtga
     4921 gatcgaaaag aacgaagtac gtatccgagt aattttcgct actccaaaac caggaacaga
     4981 aattgtcgac tttagaggat taagtccatt tatcgatgaa gaattttcag aagaatggcg
     5041 cctattcttg ccaagagaag aaggaagcca acgcggtgtg gtatttggcg aatttcaatt
     5101 aggaaatatg attgtcggta cgatcgaaga aacgggcagt ttggtgacag agtatcaaaa
     5161 aggagagtta gtctgcactt atggacccat tcgtgaaacg ctgattacaa acggtgttga
     5221 taattataaa ctacgtaaac ttgatcggtt ggatcaatgg aaaaatgcag tatgttacga
     5281 tcctgctcaa ttcgctttag gtggtatccg ggaagcacat gttcgagcag gtgatgctgt
     5341 agcaatcttc ggtttaggcg ctatcgggca aattgcgatt caattagcta aaaaagcagg
     5401 tgctactacc atcgttggga tcgatcccat agcgcagcgt agagaattag cagaaaaaca
     5461 cggtgccgat tacacgctag atcctatgaa agcagatgtc ggttttcgtc taaaggaatt
     5521 gacagataaa cgtggagtag attcagttat tgaaacgagt ggcagtgctg ccgcattaca
     5581 agcttcttta atagggatcg gctatggcgg gatcatttct tatgttgcct ttgctaaacc
     5641 ttttccagct gggtttaatc ttggtcgcga agcacatttc aataatgcaa aaatcgtctt
     5701 tgctcgagca tctagtgaac caaacccaga ttacccaaga tggaaccgta aacgaatcga
     5761 ggacacatgt tgggaattat tgatgaacgg ttacttggat tgtgaagaaa tcattgatcc
     5821 agttgtgcca tttgcagaaa gcgcagaagc ttacatgcgt tatgtagacc agcacccaga
     5881 agaaagtatc aaaatgggcg ttcaagtccg aggaggagaa caatgaaatt cggtacgcaa
     5941 gatcaagcat ttttcccaag agacatttta gaaaaattta gatttatcaa agactgtgga
     6001 tttgaagaat acgaaatcga cggtaagttg ttaatagaaa atatcgacac agtcaaacag
     6061 gcaatcaaag aaacagggat cccagtagtg acagcttgta acggctacga tgggtggatc
     6121 ggtgacttta tcgaagaacg gcgtcttaat ggtgtacgtc aaattgctga gattttgcgt
     6181 gcactaagcg aggttggagg caaaggaatc gttatcccag cagcttgggg aatgttcact
     6241 tatcgattac cgccgatggt ttcaccccga agcaaagaag gagactataa agcagtcagc
     6301 gaatcacttc gttatttaga taaagtagcc gaagaaacag gtacggttat ttatttggag
     6361 cctttgaacc gataccaaga ccatatgatc aatttgttag cggatgcgcg tcattacatt
     6421 gactctaatc aattgaaaca tgtgcagatc atcgctgatt tttatcatat gaatattgaa
     6481 gaagatgata tttcaaaagc attgactacg caaaaagagt atgtgggtca tatccattta
     6541 gcagataatc atcgctacca accaggatct ggcgcaattg attttactcg ccattttcaa
     6601 acgctacttc aaaatgagta tgaaggttat atggtatatg aatgtcgttt gcgaggagaa
     6661 aatgaagtga aaatttatca ggaatcattg caatacttga aaaactgtct ggaaacagcg
     6721 cgtcatttaa atgatttaga gaaagaatag agaaagaata acaataagta aataagcaaa
     6781 ggagagagaa ataaatgaag aaagtagcaa tcataggcgc tggacaagta gcagaaaaag
     6841 tacatgctgc ttattatcaa aaaagatcag atgtcttaga attggccgct gtggttgatc
     6901 cagtcagcca gcgagcagaa gacttttgcc gacgtacagg attttctcat gcgtatacat
     6961 cagttacaca aatgctgaat gagatttctc ccgatatcgt gagtgtctgt acacccaatc
     7021 gctttcactt cgaacatgta ttggctgccc tcgaagctaa tgcttcagtg atgtgcgaga
     7081 aaccaccagc tatgacagct gatcaagcaa gaaagatggc agagacagcc gataaaaacg
     7141 ggcagatttt ggcttatgga ttccagcatc gtttttctga cgaagcaaca atgatcaaga
     7201 atcatcttga tgttctcggg gatatttatt acgtagaagc gaatgcgctt cgtcggtctg
     7261 gtgttccagg ctggggaacg tttatcgaca aagaacttca aggaggcggg cctttgatcg
     7321 attatggcat tcacatgctt gatacagcga tgtatattac tggatttcct aaagtaaaaa
     7381 gcgttcgggc gtacagcttc caaaaaattg gtacgaaaaa aaattcaggg aaattcggga
     7441 aatgggatcc aagcaaattc acagtagaag atgcaatttt tggcacactg gaattagaaa
     7501 acggcaagat catccaactg aatacttcgt ttgctttgaa tatcagagaa gacaaactga
     7561 atgtggcttt ttgtggcgat caggcaggtg ctaatctgta tccaggcgaa ctattcgaag
     7621 atgtagatgg aaagctggtt tccttacaaa agaccacgcc agttaatgac ccacatgaac
     7681 taagtatggc tcattttgtg aatcgggcgc tcggtgacca gactcagatg attgcagatg
     7741 gctggcaagg atatgtcatc cagaatctgg tggaaaattt atatcgttcg gcagaaacag
     7801 gagaagttat taccttatga aagtagaaga aaaacagttt gatttagagt atatcaataa
     7861 gtatgcgagt cagatgacca ttggaaatgg attttttggt atcagaggaa gccaggagga
     7921 agactatcgg aatcaagtaa ggggaatgtt tgcagctggt gtatataacc gacctttagg
     7981 agcagaatca gccgagttag tgaatctccc agatgtcatc agatgggaaa taaaaatcga
     8041 cggtgaacat ttttctcttg agacgatgaa agttcttgca tacgagcggg cactagattt
     8101 agaaatggga gagcttaaac gctatgcgct cattgaaact gctcaaggaa aaaaagtgga
     8161 agtcattact tgtcgtgttg ctgatcaaaa taatctccat caggcagccc tgaaagtggt
     8221 catcaagccg ttgaccgaag actgccaagt agatgtgaaa acgggtatcg atggacagca
     8281 aaccaatttt ggtacacagc agttggttga aaaagaactg cgagtatttg gtgacgattt
     8341 gatcagtgca tcctaccaga caacagaaag taaaattgat attgggattg cagctaaatt
     8401 caataaagca ggtgtattcc aagcaaaaaa tcgccggatc accgcttcct tttcagaaaa
     8461 gatcagaaaa aatacgtcct ttacgctgga gaagatcatc gtgctctcta cctctcttca
     8521 gcaaacagac ccgtttaaga aaagtattgc ccaagcccag aaaagcgtta gttatacaga
     8581 aattttagag cagtcgatgt cttattggac agatttttgg catcagcatc ggataacgat
     8641 ccaaggcgat tcttctttcg atcagttagc gatggacttc gctgcttacc atttacagat
     8701 tatgactccc cgcaatgatc caagtgttag tgtaggggcg aagggactaa ctggcgaagg
     8761 ttataaaggg catgttttct gggatacgga aatttttatc ctgcctttct ttttatatca
     8821 gtttcctgaa gtagcaaaga atttgctgct gtaccgttat gatcgattag aaggcgcacg
     8881 gcaaaaagca agagattatc actatcaagg tgcacttttc ccatgggaaa gcgcacacac
     8941 tggtcaggaa gagacaccca aatttgctgc attgaatatc aaaaccggaa caagacaaga
     9001 agttgcttca gctgccgcag aacatcatat cagtgcagac cttgcttatg ccgttcaaga
     9061 gtatgtccaa gcaacagagg atcaatcgtt tttagaaaca aagggaaaaa cgctcattca
     9121 tgaaacagca gctttttggc tgagcagagc tgtaaaagtc gatgatcatt tagagataca
     9181 cagagtcatt ggtccagatg aatacacaga atatatcgac aataatgcat atacgaacta
     9241 tctggcgcat tataatgtgg ccttagctgt taaatatcaa gctggcgatc cgcttttttt
     9301 gaaagaatgt caaaattttt tagataagtt atatttgcca gtgcctaaca aagaacagct
     9361 gattccgcaa gatgacacgt atctcagtaa gccagttatt gatttgacca cttacaaaga
     9421 acgtcctggc agccagtcta ttttacttga ttatgcccgt agcgaagtaa atgaactcca
     9481 agtcagcaag caagcagatc ttgtcatgct gttatactta ttacctgagt atttttctca
     9541 accaatcgtt tctaagaatt tgcattacta tgaaaacaaa acaatccacg actcatcgtt
     9601 gagtaaagcg attcacgcca tagaggcttg ccgtatccaa gaaatggaat gggcgtatca
     9661 tttattccaa gaagcgtgcc tgattgattt aggaccagat ccgcattcat cagatgacgg
     9721 aattcatgct gcttcattag gagcactatg gctagcagtt atatttggtt tttcaggtat
     9781 tacagtaacg aatggaactc tttctatttc tccaagactt ccttcagcat gggaaaaaat
     9841 ttcttttcct tttatttgga aaaatcacag gttgctattt gttttgacaa aaaaacaaat
     9901 agtaataaaa aaagagacga aagaaacatt acctattttt gtcaatggag accaatacca
     9961 gctggaaaaa gaactgatct tggaacggag gggaaaataa tgcgtattct gattgcgcca
    10021 gattctttca aagaaagtct gacagcctta gaggcagcac aagccattca atcaggtttt
    10081 caagaagttt ttgttcaggc tgaatatgat ctgctaccta taggcgatgg aggagaagga
    10141 acggtttcat cattagcaga cagtttaagg ttgaagcggc aaacagcagt cgttactaat
    10201 gcatttggca aaaacaaaga aatccagtac gcttcagacg gtcaaactgc gatttttgaa
    10261 atggcagaaa tcgtcggatt gaaagatgtc ccactaaacc aacaacagcc acttaggtta
    10321 tccacgcgag gagtaggaga actgatacga caattgttgc aacaaggtgt tcgacacatc
    10381 atcatcggcg ttggcggaag tagtaccaac gatggcggca tcggcatggc agaaggtcta
    10441 ggttatgctt tttacgatca agataatcaa aaagtagaag cactgggaga aaatctaggt
    10501 aaaattgcta cagttgataa aacgaacgtg cctattgagc tgattaaagc caaaatcact
    10561 gtcattactg atgttactaa tcttttatgc ggaaaagaag gcgcttcttt tgttttcgga
    10621 ccacaaaaag gcttgaaaga aaatgaatta gcagtggctg accaagccat gcatgatttt
    10681 tatatgagat ttgcccctca agtaatcgag gcatcgggca gtagtgctgg tggtggaatg
    10741 ggtgcaggac tgttagcatt tgcttcaggg aaaatagtct ctggcatcga ctacgtattg
    10801 gatttactgg agttcgataa acgggtacag caagcagacc tcgtaatcgt aggcgaagga
    10861 cgtctagatg gacaaagtct aaaaggaaaa gcgccagttg ggattgctag aagagtacca
    10921 gagggcgttc ctgtgatagc tatttgcgga agtgttggta aaggaagcga aaaagcagca
    10981 gatgttggca tttcggccgt ttttccgatc cttcctgcag ctgtgccttt acaagaagct
    11041 ttacagcaag ccactgataa tcttcatcgc acagctaaaa acatcgcatc tttacttcaa
    11101 gtcgttcatt ttcaaggagg taatcgatga aaatcaaagc agcactattc gatttagatg
    11161 gcgttcttgt agacacagct cgttatcatt acgaagcgtg gctggtttta gctaaccagc
    11221 tgtccattcc attcactgag aaagaaaacg agcaactgaa aggaatatcc agaaccgagt
    11281 cactagagcg attgctgtca tttggaaaaa tggaacagaa attctcagaa aaagagaaat
    11341 cagcctttgc tgagcaaaaa aacaatctat atcttcaagc gattcaaaaa atggatgaaa
    11401 catccgtttt gcctggtgca atagctgttc ttgagtactt gaaaaaaaca aacatcaaga
    11461 tcggattggg atcagcaagc aaaaatgcac gcctcatttt agaaaaaacg aatttgacaa
    11521 gttattttga tgtccttatt gacggcacgc aagtatcaaa agcaaaacct gatccagaag
    11581 tttttctcaa aggagcacag caattgaatg taccgccaaa tgcatgcttg gtcattgaag
    11641 attcagaagc tggttgtcaa gcagctttgg ctggtaacat gcatgtattg ggaataggtg
    11701 aaaacataaa tcttccttct gctgaatatg tgattcctga tttaaccgtt tttgatcaag
    11761 tacgttcgtt ttggcattta tcagaagccg tatcacgtat ataatagttt ccgttttttc
    11821 tgtcacaagc aaaacgttat cctccttttg gaaatcaagg tatgatgaga aaaaaggggg
    11881 attttatatg acaaaaaaag cagttttcgc aggcggatgc ttttggtgta tggtcaaacc
    11941 atttgaagag cagccaggga tcatttctgt gacatccggt tatactggcg ggcatgtacc
    12001 aaatccaact tatgaacaag taacaacagg cacaacaggg catacagaag cagtagagat
    12061 cgaatatgat ccagaagtta tttcttatga tcagttggtg gaaatctatt ggcagcaaac
    12121 cgatccgact gacgcattag gacagttcgc tgatcgcggt gattcttatc gtccagtgat
    12181 ctactataat gatgaagaag aacgaaatat tgcagagcgt tcaaaacaag cattgcaaga
    12241 cagcgggcga ttcgataagc caatcgtgac aaaaatcgaa ccaagaagcg aattttatcc
    12301 tgcagaagat taccatcagg attattacaa gaaaaacaag ctccattaca atctttatcg
    12361 agaaggttct ggacgtgcag gtttccttcg tcgacattgg ggcgtcaaga aataagaagc
    12421 cgaaaaagct acttatggaa caccgtatat cagaggttgt aaaaccagca ttttgagcta
    12481 atactgaggg tcagctgatt gaacaccatt tagtggagag gttgtgaacc tgtcgcctag
    12541 ctccaagtca caaggatttg gtaagagaac actatatatc agaggttgtg acaagtcgtt
    12601 tgtcacaacc ttttttcaat acctaagatg aaggacaatc agcaacgcac tgctcaaaaa
    12661 aggaatgaaa ggcagctttt ccaacttttt tttatgacag ataaagtaaa cagaaaaaac
    12721 ggatattcca aacagactag caagaaacaa gatctgaagc aactcttcta gagacaagcc
    12781 accactccaa gaaagcatca acagagtatc gccagctccg agataggatt gaccatagcg
    12841 aaggcacaca ccgcctacga taaacgttaa cagcacactt tgccattgga agacgccggc
    12901 ctgcaaccaa aaaatccata aaattgccca agaacagaat agcgtgtgag aatggaccat
    12961 gaagtaaaat aaatccatca acgaaaatag caaagccgaa agcatccata tgaacgtcga
    13021 aaaatttctt gcggaagaaa aagtaaagaa ccaggcaaaa agaccaccac ataaggcttc
    13081 tgccaacaaa tagtagaagg gcaaccgaca cctgcaatag cggcagcgaa atcgttggac
    13141 gatgatcgag agaatcggaa tcaattcata ccaagaaagc gaacgacagc atttgacaca
    13201 atgagatcta ggataaataa tagagtgacc taacggaatc cgctgggcgg ttaaacataa
    13261 aaatgatccg atacagcaac caatgataaa ataaagtaac acgccatctc ccccttttca
    13321 aaaccaagat acgcaagctt aaactaaatt tttgtacata cataaaattt gaggtgaaaa
    13381 aatgaacgtt tcaatcggat tagatatcgg aaccacacaa accaaagcag ttgctttcac
    13441 agatgaagcc caagaagcag catccgctta ttttcgctac cctttgatcc aagaaacaca
    13501 agggatggca gaacaagatc aggaattgat ctttcaaggt gtctgcaccg ttataaaaga
    13561 agtaattaaa cagttgcctt cagacacaat cattcaattt gtctccttct cctctgcgat
    13621 gcatggcctg atattgttgg atgaagagaa aacaccgtta tctcgaatga tcacttgggc
    13681 agataaccga gcagcggacg aagcagaaaa actgaaatat accgtgcttg gcaaagaatt
    13741 ttatcggcag actgggacgc cgattcatcc aatgacacca ttgaacaaaa tcaagtggct
    13801 gcaaaacagc caacctgaat tattcaaaaa aactgcttat tttgtcggta tcaaagacta
    13861 tatcttttat agattgtttg gcgaactgat cagcgactac ggaacagctt caggaactgg
    13921 atattttgat atccatgaat tccaatgggc agaagaagtg cttgactact tgaacattcg
    13981 aatagagcaa ttgccgcaag tctttccttc cacctatcaa gtaacaggtc taccatcaaa
    14041 aacagcagca gaactaggtt tgccaaatga tatatcattt gttttgggag gggcagacgg
    14101 gccgctttct aatctagggt taggtgcttt tggtgatacg atcgccgctt tgaccgtagg
    14161 aacgagcgga gcacttcgtt ttattactac tcagcctcag ttccatccag aaatggagac
    14221 gttttgttat gttctggatc agaaacattg ggtagtggga ggagcgacta gcaatggtgc
    14281 tggcatcttt gattgggcag ggcatacatt tttacaggaa gtcgttcaat ctgctaaaga
    14341 aaaaggagaa aatccatatg atgcactgct ttcggctatc gcttttgtgc cagcgggtgc
    14401 caatggtctg atttttcatc cctatttact tggtgagcga gcaccgcttt gggacgcaga
    14461 tgcgtttggc agttttatcg gtttacagcg gcaacataca gaaaaagaaa tgatgaaggc
    14521 gattctagaa gggatctgtc tcaaccttta tcggatttta aaaggagttt gccaggaaaa
    14581 tcagctcaca gagatccgtg ctacaggcgg gtttgctcaa tctccgattt ttcgtcagat
    14641 gatggctgat gtactgggtt acccgcttgt tttccctact gtcacagaag catccgcatt
    14701 aggagctgtg attttaggct ggagaagcat cggaaagatc caaagtatca gtgaagcaca
    14761 ggaattgata gaattgaaag agcaagttgg agtacaagaa actgatcata aaaaatatca
    14821 gaaaatctat ccattatttg tttcgacaca aaagcattta gcacagactt atcagcaatt
    14881 tgctcaactg cgagaagaat tagctgattc tgaaaaatga ttggctttac ggataaaaaa
    14941 gcgaactgaa aatcgttctc aggtaaatga ttttgttgta cttttcattg aaagtctact
    15001 attattttaa gtgtaagaaa atgttaggag gcaagaaaga tgaaattcca acaaaccaaa
    15061 gaagtattaa atcaattagt tgcagatcta agccaaatgt ctgttgttgt tcaccaaaca
    15121 cattggtaca tgagaggacc aggtttcctt acattgcacc caatgatgga tgagttcatg
    15181 gatgatttga acgctcagtt agatgaagta tccgaacgct tgatcacatt agacggttca
    15241 ccttattcta cattgcgtga atttgcagat aacacaaaaa tccctgatga aattggccgc
    15301 tgggatcgta ctattccaga acgtttggaa acacttgttg caggctatcg ttatctagca
    15361 gatctttacc aaaaaggaat cgaagtttct ggagaagaag gcgacgatcc aacacaagat
    15421 atctttatcg aattcaaaac agccacagaa aaacgtatct ggatgatcca agctcatttg
    15481 ggcaaagcgc cagagatcga cgcgtaaaaa ctgttgttga acttgaccga aaaagaggtt
    15541 gtgaaatcag cgttttgacc tgagtattag agaaacaaac gtcaaaatag ttcctcatat
    15601 tttgccgttt tttgagctaa taccgagggt cagctgattg aacaccgttt accaagaggt
    15661 tgtaagcctg ccgttcagct ccgagtcaca agaaacaatt ttaaaaaaca gcttcttatg
    15721 ttttatggat attgtgactt gtgaccgagg agttggcagg agaacaccgt ttattcaaaa
    15781 taaggggttg tgacaagact tttgtcacag cctctttttg tttttcattt tgacaggaaa
    15841 cgaccctttt atataaaaga aagaaaagaa gtaaagtttc atataaattt ttcaatcaag
    15901 cagaaaaacg ttgacattga ctagattagc gggtaatatg ttaaatggta ttgtttgccc
    15961 cacaaagagc cccggaaact cgaagtgtat gtcaaacatc agaacttaaa ttggaggaaa
    16021 ataacgtgcg tacaacatat atggccaaac caggcgaagt agaacgtaaa tggtatgtag
    16081 tagacgcaac tgatgttcca ttgggacgtc tttcaacagt tgttgcatct gtactacgtg
    16141 gtaaaaataa accaactttc acacctcatt tggatactgg cgatttcgtc atcgtcatca
    16201 atgcagacaa agtgaaatta acaggtaaaa aagcgactga caaaatttat taccgtcaca
    16261 gcaactaccc aggaggattg aaatcaatca ctgctggcga attgcgtgct aaaaattctc
    16321 gtcgtttgat cgaaacttct gtaaaaggta tgcttcctaa aaacacttta ggccgcaaac
    16381 aatttacaaa attgaacgta tacggtggag cagaacatcc acatgcagca caacaaccag
    16441 aagttttaga catcacaaac ttaatctaat aggagggaat ttcattggca caagttcaat
    16501 atatcggcac aggccgtcgt aaaaacgcag ttgctcgcgt acgtctagta ccaggaactg
    16561 gcaaaattac tgtaaataaa aaagatgttg aagaatacat tccacatgct gacttgcgtg
    16621 aagtaatcaa tcaacctttc gctgttactg aaacaaaagg cgcatatgac gtatttgtta
    16681 acgtaaacgg cggaggatac gctggacaat caggagctat ccgtcatggt atcgctcgtg
    16741 cattgctaga agttgatcct gacttccgtt tagctcttaa acgtgctggc ttacttactc
    16801 gtgacgcacg tatggtcgaa cgtaaaaaac caggtcttaa gaaagcacgt aaagcttcac
    16861 aattctcaaa acgttaatat cctttatata tcaacgtttc tggcactttt caattatttg
    16921 gaaagtgctt cttttgtttt taagaacaca tttaagaaca catatcaatc acacttattc
    16981 aatcctctct gaaaagcttc aaaaacagcc ttatcaacta tatctggtgt gttcatatat
    17041 ctttgttgta gaaatatctg agtgtgttaa agcatttgaa atttgattca tatctgctcc
    17101 accttgtcta gctatagttg cataagtatg acggagtttg tgtggagata aatgaactaa
    17161 gtcaggatgc cttcttttaa ttgaattgat tcgatagttt aggtaatcgg catgaacagg
    17221 acaatttact tctccagatg gtttagtata agtaaagaga tactgatcta acgtttgctt
    17281 aatttttaat ttcaacaatt gatcagcttg taccgatttc cattcactta gcaatgtcat
    17341 tacaacttct ggtacttgaa tgacggtatc ttttcgacct tttgtaaact tttttctttg
    17401 atatttatct aaagtatgtt taagacggac cgtttgtttt tcaaaatcaa tatatttcca
    17461 ttgcaaagcg taagtctcac tttttctatc accaagatac agggtaatca taaaaagtgt
    17521 gtaatcatga aaagtaagta aatgattatt aaaatcatca tttactgctt ctatccaatt
    17581 agtaagttct tttgatgata gtgcttgctt accttcttgt attctttttt cttctaaagc
    17641 atttttttta ggtgcagtga tactttgaat aattttagta gttcgattaa attcgatata
    17701 atttagtatt tctgcaatat cgaacatgga acgaacatat ccttttatga tttttatatt
    17761 tgcatattct ttcgattttt tggttaattc atctgatatt atctcagtat tgatatttag
    17821 ataattcatt gcgtattcac caaacatagg taaaatatgc aaccgaaata tatcttttgt
    17881 attactaatg gttatatcac taggaggcct gtttgatcta attgtttgac ctaattcata
    17941 tctaggaagc catttagatt cgtaaaattt tttaaacgtt atttttcctt ttaattctaa
    18001 tgagtttgca ttctcctctc tcaaaacttt ttctattttc ttcttcatat cattttcggc
    18061 tttaatcgcc aattgcctat ttggaaatgt tttttgatac ttttctgatg caattcctgt
    18121 tatttcttta aacctttttg gatatctcag attagtttta tatttcccat ttgaaagctt
    18181 tgtaggcaaa atcctcactc cttaaaaaaa gtaaggcagt atatttcact aattttagta
    18241 tatgcggcga
//
LOCUS       DACDMM010000047        18061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDMM010000047 DACDMM010000000
VERSION     DACDMM010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.047"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            11..733
                     /locus_tag="IWD16_001222"
     CDS             11..733
                     /locus_tag="IWD16_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9088298.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            791..2830
                     /locus_tag="IWD16_001223"
     CDS             791..2830
                     /locus_tag="IWD16_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP9088299.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2939..4090)
                     /locus_tag="IWD16_001224"
     CDS             complement(2939..4090)
                     /locus_tag="IWD16_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9088300.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4244..4774)
                     /locus_tag="IWD16_001225"
     CDS             complement(4244..4774)
                     /locus_tag="IWD16_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088301.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4873..5223)
                     /locus_tag="IWD16_001226"
     CDS             complement(4873..5223)
                     /locus_tag="IWD16_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP9088302.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="IWD16_001227"
     CDS             complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="IWD16_001227"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP9088303.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="IWD16_001228"
     CDS             complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="IWD16_001228"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP9088304.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7626..8288)
                     /locus_tag="IWD16_001229"
     CDS             complement(7626..8288)
                     /locus_tag="IWD16_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9088305.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8472..10118
                     /locus_tag="IWD16_001230"
     CDS             8472..10118
                     /locus_tag="IWD16_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9088306.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10109..11191
                     /gene="uxuA"
                     /locus_tag="IWD16_001231"
     CDS             10109..11191
                     /gene="uxuA"
                     /locus_tag="IWD16_001231"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9088307.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11299..12852)
                     /locus_tag="IWD16_001232"
     CDS             complement(11299..12852)
                     /locus_tag="IWD16_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9088308.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            13000..14289
                     /locus_tag="IWD16_001233"
     CDS             13000..14289
                     /locus_tag="IWD16_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9088309.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEAAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(14415..15494)
                     /locus_tag="IWD16_001234"
     CDS             complement(14415..15494)
                     /locus_tag="IWD16_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9088310.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15603..16043)
                     /locus_tag="IWD16_001235"
     CDS             complement(15603..16043)
                     /locus_tag="IWD16_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9088311.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16100..16510)
                     /locus_tag="IWD16_001236"
     CDS             complement(16100..16510)
                     /locus_tag="IWD16_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9088312.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16691..17125)
                     /locus_tag="IWD16_001237"
     CDS             complement(16691..17125)
                     /locus_tag="IWD16_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9088313.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(17225..18052)
                     /locus_tag="IWD16_001238"
     CDS             complement(17225..18052)
                     /locus_tag="IWD16_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9088314.1"
                     /translation="MWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGILLEAVLG
                     FDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITSDRLLSVI
                     FGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVSLNLFVFG
                     IEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIGRGLTLLE
                     AKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGFTYHPIE"
BASE COUNT         6224 a         3359 c         3263 g         5215 t
ORIGIN      
        1 attatctatt ttgttactgc ttacattagt agttggctat ttttcgattg agtttatcca
       61 tggattttcg tctgcaaaac agagttcaac tgtgaaaaag gtagagccga aaagtatccc
      121 taccacacta aatgtggctt tgattggttc agatgctcgt tcgaaagacg aaaacggtcg
      181 ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa aactgatctc
      241 tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga taaatgcagc
      301 ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt tcaaatttga
      361 agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta atgaactgtt
      421 tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg tatatataaa
      481 caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat ttcgggaaga
      541 caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag ctatctctca
      601 gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga aattacttgg
      661 tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg atttcctcaa
      721 agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa
      781 ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc
      841 cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca
      901 agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa
      961 caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc
     1021 attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg ctgatgtcaa
     1081 aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc
     1141 atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac
     1201 attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga
     1261 aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat
     1321 agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc
     1381 aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt
     1441 cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta
     1501 ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat
     1561 tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta
     1621 cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa
     1681 aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtgg
     1741 gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt
     1801 tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa cagcttttga
     1861 cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct
     1921 tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga
     1981 agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg
     2041 ttatgctcct ggatctacgt ttaaaataat tacagcagca atcggtctcg acaacggcac
     2101 tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg
     2161 gggatctttt caaacaacgc gtgtcagtag tgatgtatca caagtagact taaaaactgc
     2221 tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa
     2281 atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat
     2341 gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac
     2401 tggatatgga cagggcgaac ttctaattaa tcctatccag caagcagcaa tgtattctgt
     2461 ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga
     2521 taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc tgagagaagt
     2581 tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc
     2641 gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag agaacagttt
     2701 cttgtttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa
     2761 agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa
     2821 ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt
     2881 ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt tgatttactc
     2941 acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata agtgtactta
     3001 ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga ctttgctgtc
     3061 caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca ccgaccatca
     3121 atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca agcaatttgg
     3181 tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta aagtcagaaa
     3241 aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca tatcctaaag
     3301 cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct atcccagcaa
     3361 aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac attcctaaac
     3421 aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct gcaaatgaaa
     3481 gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa attagctgtt
     3541 caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga aaagataggc
     3601 tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc aaaattgttg
     3661 atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa atactgacag
     3721 aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt
     3781 tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca acgaacatac
     3841 caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct aaaaacatta
     3901 ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg acccaattca
     3961 atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct gggataccta
     4021 atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa
     4081 taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc ataacataca
     4141 ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa gatttttttg
     4201 gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt atagtcattt
     4261 ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa ataaagccga
     4321 atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg aatccgccaa
     4381 tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc atgctgaaga
     4441 acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc aggatagaaa
     4501 cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa agaccactga
     4561 acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca aaaagattca
     4621 acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga aaaacagcag
     4681 aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt aataaggcgt
     4741 atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt tgacttattt
     4801 taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag gagaagaata
     4861 tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg ctagctcatc
     4921 gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt cttctactcg
     4981 tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag gggcttccgc
     5041 aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg tttgggaata
     5101 acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt ttctctccgc
     5161 ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc
     5221 cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata taagaaaaag
     5281 agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat
     5341 ccttttttcc gctctctgac tttttacaat cttcatagct caagtgcgca tcagataatc
     5401 gaatgctcct aaagctgcag tagctcctga acccattgaa acaatgattt gtttgtatgg
     5461 actatctgta caatcaccag ctgcgaaaac gccggggata ttcgttgcgc catttttctc
     5521 ggtcactatt tcatttcttg gattcatttc gatcgttcca tctagccaat ccgtattagg
     5581 gagtaagccg actagaatga atactcctga aacagctagt gtatgcatct ccatcgtacg
     5641 gcgatcgata taggagagag attctacctg attcgtaccg ctgatttctt tcgtcgctgc
     5701 atgtttgata acagtgacat tcgcaagaga gactagttta tcctgtaaga tttgatctgc
     5761 ttttaattct ggtaagaatt ctagaacata tacatgtttt gctaacccag ccaaatcgat
     5821 tgctgcttca acaccagaat taccaccacc gataacagca atctcttttc ctttaaataa
     5881 agggccgtca cagtgtgggc aataagcaat tcctttattt ttaaactctt tttcgcctgg
     5941 tacattgata gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga
     6001 tataccgtta gctaagccaa tattaataaa atgttgctct ttatttaagg aaacggcctg
     6061 ttgattcatc ataatgtcga tagggtaact acgcacatga ctttctactt gctgcatcaa
     6121 ttttggacct tctgtgtaag aggtaccaat catattttca atccctagtg tttcaacgac
     6181 ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt cctttacgtg ctgcatagat
     6241 agctgcacta ctagctgctg gtccaccgcc aattactaat acatcataag gttctttttc
     6301 aaacaactct tcttcaatca aagggccagt gattttttca acaatttgtt cgatcgtcat
     6361 gcgtccgcta ctaaattctt ctccatttaa aaacacagtt ggcactgcca tgattttttt
     6421 atcatcgatt tctttttgga acatcccacc ttcaatcatc gtatgagtaa tatttggatt
     6481 taatacagct aatatattca atgcctgtac aacgtcgggg caattatgac aagtcaagct
     6541 aacataactg tcaaaatgca atgtttgtgt tatttttttg attttaagac tcaaagattc
     6601 actgattttc ggtgcccgtc cactaatttg taacatagcc agtaaaaaag aggaaaattc
     6661 atgaccgagg gggataccag caaaaacgat tccactttct ttgccaactg gattgataga
     6721 aaaactaggt gtgcgcatcg aatctctttt ttcccaagag attttaggag acatttctgc
     6781 tatttccttc gtaaattcag ctagtttttg actattttca ctattatcca aactaacaga
     6841 aaaaacaata ggagattcaa tcagtgctaa atattgttcg agattttgtt tagtttcttt
     6901 gtctaacata tccaccacct aaatctttcc aacaagatca aaacttggtt tcaatgtttc
     6961 gccactttct ttccattttg ccggacaaac ttcacctggg tgggtacgta catattgagc
     7021 agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg attccatctg cattgatttc
     7081 catagcttga acgattccgt ctggatcaat aataaatgtt cctcgctgag ctaatccttg
     7141 ctcttcatcc aaaacatcaa aaatacgaga aatctgatga gacggatcac cgatcatcac
     7201 gtattcgatt ttaccgatag catctgatgt atcatgccat gctttgtgag taaagtgggt
     7261 atcggtagag caagaaaaga cttcaacacc taaagatttc aatgtactgt attgttcttg
     7321 taagtcttct aattcagtag gacaaacaaa agtaaaatca gctggataga agcaaagaat
     7381 actccagttc cccataagat ctttttcact gacttctaaa aattctcctt gatgataagc
     7441 atgtgcatta aattcttcga tttttttccc aatcaatgac atagtaaaac ctccaataat
     7501 aattatttat ttataattat tgtaatttag ttggagttgc aaagtcaatg gatattttgt
     7561 tatttaataa agattactca aaaacgaagg ttaattataa aaatagaaga ggtcgcaaga
     7621 aaatgctact gctggaaata ctcgggataa cgttgagtaa cagtttcctg ttctttcagc
     7681 atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt atcaactaaa
     7741 gccttgagta aatcataatg ttgttccatg atcgtttccc atggaagccc ctgaatggtc
     7801 actcgtaacc agcggaaacg ttctaaatgg gtattatggt catctaacca gttccaaact
     7861 tcttttcttc ctgcaatttc gaaacaagct tcatgaaata aattatctgc acggaaaaaa
     7921 gcgcgttcgt catttatttt gactgctttt tcttgttctt ctaaaatcgt ttctagcact
     7981 attcgtgttt gtgtgtccaa tttggaacaa cattcaatca tgatttgctc ttccaactgt
     8041 tctcggacaa agcgagcatt ttctgcagaa acaagattaa ttttggaaac atacgtacca
     8101 ctttgaggta tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc ttcgcgaatg
     8161 ggagtccggc cgatagcaag atgttcctcc agtgcctttt cagatatttt ccttccaggt
     8221 tctaagtcag catagataat ttgtttacga atcgcttgat aagcttgtga ttgcaagctg
     8281 cttttcatct ttattcctcc aaaatcattt ctatgaacgt agtataacag aagaggtctg
     8341 aaaaacaatc tggtatgtca cattaactag taaatttgta taaatagtaa aattttggtt
     8401 gacatagcga tttttattgt ataaaattca cttgaataca aaaactaata tgttagttta
     8461 ggaggggaaa aatgattgat ttaaacaact ggaggaactt tatccctgaa ttatctcaaa
     8521 aagaagtaga aatacctcat tttgaccgtg catcattaag agaaaatgga caaaaaaatc
     8581 ctgtctggat ccatttcggt ggcggaaatc tttatcgagg atttcatgca gaaatcgctc
     8641 aacgtttagc ggatctaggc gaattgtcca ctggtgtcat tgtttgtgaa acattcgatg
     8701 aagaaataat tgaaagggct taccaagatt ttgatgattt gattcttgaa gtggtcatgc
     8761 caagcagtgg agagttcgtc aaacgtatat tagctgcgac agctgacagt ctgtattgtc
     8821 atccagattg ttctgaacac tttgataaga tgattcgaca ctttgaaaat cctagtttac
     8881 aattagttac ttttgctatt actgaaaaag gttatcgatt gaaagataca aataataaat
     8941 atataggacc gattgcagtc gatataaaaa atggtccaaa gaagccatca catacaatga
     9001 gtattcttgc tgcgctactt tataaacgtt ttttatctgg agcttggccg attgcacttg
     9061 tctctacaga taatttttca caaaacggta gtcaattacg tacttctttg ctaacgatcg
     9121 caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt tatccaatac ttatcgaatg
     9181 aagaaattgt tgcgtttcca tggacgatga ttgatcgtat caccccgaat ccttccttgt
     9241 cagttagcaa aatgttggca aaagagggct tttcaaatat gtcgattttc caaaccgcaa
     9301 aaggaaataa tattgctcca tttgtcaata cagaggacat ccattattta atcatggaag
     9361 atcattttcc caatggacgt ccgaaattcg aaaaagctgg ggtattcttg actgatcgtg
     9421 caacggtaga taaagcagat acgatgaagg tgacgacttg ccttaatcct ttgcatacgt
     9481 ctttagctat tatgggttgt ttactggggt ataggagcat agctgaggag atgaaagatc
     9541 cagatttatc gctgctagtt tctgaaatcg ggtacaatga aggcctccca gttgtggagt
     9601 ctccaggtat cattgatcca aagatctttc tggaagaagt aattactgtg cgcttttcta
     9661 atccaaatat tcctgatatg cctcagcgaa tcgccacgga tacctctcaa aaggtggcga
     9721 ttcgttatgg tgagacgatt aaaaaatata tggaaataga agttaaagac ccgcagacac
     9781 ttgtattcat accattgact ctcgctgctt ggcttcgata ccttattggc attgatgatc
     9841 aaggaaaacc attcatcccg agtccagacc cactattgac agactttcag cataagcttt
     9901 ccgatattca actgggtacg caagatacat cacatattca tgagaaaatg gctcctattt
     9961 tgtcaaataa agaaattttt ggtgtagatc tttaccaagc agagcttggc agaaaaatag
    10021 aagaaatgtt catcaagatg gtagcaggac cgaatgccgt acgaaaaaca ttacaggatt
    10081 acttacaaga acatggagga aatttatcat ggaaatgact tttagatggt acggatcaca
    10141 ggaagaaaag attaccttag atgaaatcag acagattcct gcggtcaaag ggattgtagg
    10201 cacattgatg gatataccag taggagaggt atggcccagg gagaggatca aagaactaaa
    10261 agaagaaatt gagtctcgtg ggttaacact aaaagtaatt gaaagcgtca atgtgcatga
    10321 tgccattaaa attggattac cttcccgtga gcagtatatc gaaaactata agcagacaat
    10381 ccgaaacttg gcagaatatg gaatagaagt catctgctat aattttatgc ctgtatttga
    10441 ctgggtgaaa tctgaccttg actaccgctt agaagacggc tcatccacgt tagcttttat
    10501 atctgatgat attccagatg atccaagaga aatcgtcgcg cgcatcgaac attccagcaa
    10561 tgaatttgaa ttgccgggat gggaacctga gcgcttagct catatcaaat ccttgcttga
    10621 agcctatgca tcagtagacg aagaaaaact tcgagaaaat tttgcctatt ttctccaatc
    10681 aatcatccca acatgtgaag aggtaggggt caaaatggct gtacatccag atgatccgcc
    10741 atactcgatt tttggattac ctcgaatcgt aaagaatcga gaagatttag attggttgtg
    10801 ccgcatagta gacagccctt ataacggaat cactctttgt acagggagta ttgcagaaga
    10861 tccagaaaat gatgtatatg ggattttagc agaattttgt caaagagatc gaatcccatt
    10921 tgcacatgtt cgaaatatta aatttttagc taatcgagat ttctatgagg caccgcataa
    10981 atccgaatac ggttcattag acatgtaccg catactaaaa acaatgtatg acaacggatt
    11041 taacgaatac atccgtcctg atcatggaag aatgatctgg aatgagacag gaagaccagg
    11101 atacggctta tatgatcgag cattaggtgc tgtttatcta aatggattat gggaagcaat
    11161 cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa ctgtaatttt attatgtaaa
    11221 taagaaggat gttcaaattc ttgatacata aatccggagg ttgttaataa gcattcggat
    11281 ttatgtatct tatttggtct aggaagtaac ttgattttct tttttcgatt gatccatgag
    11341 tgcttcttct tttaattcaa agatatattc tgagatcctc ataatcactt ttttccagtc
    11401 ttctggtgtg atgattcctt ttaaaatata gatttttttg atatgagaag gcaattgttt
    11461 caaagggcag tcgctgataa cgatgttgca gttctccata aaatcatttt gtattttgat
    11521 cgattcattt gaaaaaattt gttctatctt aacgatcaga tctttactga tataaaaatc
    11581 acgcgtatag ttcacaccaa tcacaattcg cggctgtttt ttgtagcgat catacaaatg
    11641 tactaggtct tcaaccatcc attgcacatt ttccttttct aaaataactg gtaacaagtg
    11701 ttgattttgt tctctgaaat ccatataaaa ttgttcaatg gcggtttgga cttctttcaa
    11761 cacgctatgt tcttgataaa gcatgaaagt aggaaggggc gtatttggtt gattgaagtt
    11821 aagaaatcca ttataaagtt tatttcgtgc gaatgccaat gagtaaagaa tcttttcttt
    11881 ttcaggtata aacaaatcaa atacagtaca aaaggactcc accatttctc ttgaataaag
    11941 aacatgagga acgtttgctt tttttaaaag tttgtattgc tccaagcttg tttcttccga
    12001 ttcgattttc gtgatagaaa gtcgagctaa aatattcact aagatgcgtt gttcgcgaca
    12061 aattggcaga ttaggtcgca aaatatctac agagggatca ataaaaaagt caatatattc
    12121 atactctgtg aattcttttt gtacgtcttt cacagaagtt gcaggaaaat tctctttaca
    12181 tatttttaat agaaaatata atttatctat cataccgcta tttagtcggt caagtacttc
    12241 ttttttaaaa aaagtattca tcataaaagc cactacttgc ggatcttcat cataaaattc
    12301 tgtagcagta cttgaaaaga tgttccaata aatatctaat gctgcatatt gaatttgtac
    12361 ctcttttcca gaaaaagact ttctgccatt attagaaaag gagatagaca aaccgtacaa
    12421 tgctaaaaat gcattaatct ttttcatttt cgtatacaaa taagaggcac tatagtttgt
    12481 ttgctgactt aaactgataa tcgaatttgt ctgacagctg aacaaagaag tcaagatctg
    12541 ataactgact gattcgttgc agtatcttcc aaatagacga taatagcagt aagccggatc
    12601 aattccttca aaattcaact ttaaaaaagg tttttgctta tcaatatgca gttctgttag
    12661 attgagacgc tcaaagtctt gttctaattc tttgatgtat agtaaaaaat ttgattcaga
    12721 catatggaaa tgatctgcta aatattgtgt agtaataaag aaacgatgtt cagataggaa
    12781 aagaaaaata tctaattttg tattgctttt tttcttcaag agaaacgttt taatcgcttc
    12841 ttgagtttgc atgaaagagg ctccttttta cttaaattgt taaaacttga aaatattact
    12901 gtttttttca taaatttaga caaaaaaaca gtaaacatat aggtagaatc tctactatct
    12961 tatttcttgc atacaaaaaa atcaaggagt gagagagtta tgagatattc aaccatctta
    13021 atatcaacat ttgcagcctt aagcttatcg cttactataa atcagacagt ccacacagag
    13081 gagatagaaa cagccaaatg gacagaaacc aagtgggcca acgtcaatcg aattaccttt
    13141 caggatgaat tttcatcctt caattcagga gtcgcaaaat taagaaaagg acaaacagcg
    13201 accatcaaag caaaaattga ctattcagga gacttagcac ctatactaca atcagctgtt
    13261 acatttgaga atgttgatcc cgctttgtcg attggctatg acgagaacac tttgactatc
    13321 caacacaagc aagcgatatt taccttcacc gttacattaa atcaggactt gttaaaacca
    13381 gcgcttttta ctgtcaaggt atcagatgga ctaccgaacg atcatcatca tttaacgccg
    13441 tatagtcagc ggcagacaat tgagcaagat tcagctatcg actcaggagg agatcttgtt
    13501 gaagaaccaa cagataaacc agaaaatgaa aataagccag aagtaccgcc tacagaaaat
    13561 ccagacggag aacagaaacc ggaaatagaa cctggagaag aacccgatac agaaactcaa
    13621 ccagagccag acaatgaatc aaaacctgaa ataacccctg gagaaaaacc agatgtagac
    13681 ccagaagaaa aaccggatgt gactccagaa ccagatacgg attctggaaa ccaaacagta
    13741 ccggaaacaa atccagacac agataatgaa actgaaaatc cagaaaaacc agaggcagcc
    13801 ccagaagaaa aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta
    13861 ccggaaacaa atccagacac agataatgaa actgaaaatc cagaaaaact agaggtagat
    13921 ccagaagaaa aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata
    13981 ccagaaaaaa taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca
    14041 aatctagcaa ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat
    14101 agcgcccaat cagctgaaac cttaaaaagt agcaaggata caacctttgc tagtcctgaa
    14161 acgaaaaata aacagctacc aaaatccgga gagtcacaaa acaaagttat actgtggagc
    14221 ggaatcattc tattaagtat agcaacaatg ctttctgcaa aaagatttaa acaaaacaga
    14281 tctctctaac atttgaataa tgtctgtgaa cgcatttttc aaataaaaag aaatatccag
    14341 aaaacgaccc caaaatacca gattgaaatc tgatatttgg ggtcgttcat actttatctt
    14401 tcttcatatt tattttaagg gatatctgtt tcttttggaa gatggttcgg gatctttttc
    14461 actttcaata catctaaaaa gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg
    14521 ccaaagaacc gttcgctaac aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt
    14581 ttgctggaca tcaacttcgg attcagcaca taagactcaa ttagatggac aatcaaaatc
    14641 gtaatcagga tataaacaac atctcggatg cctccttggg aataagctat gaaagataga
    14701 ggaatacaag aaatgataac acctgctact gggatcagac ttaaaatgaa aatcatgatc
    14761 gctaaactca gcagctgatg aaagccaaat acagccaagg cgattgtagt gagcaacgtg
    14821 tttaaaagag cgataacaaa ttgagcttct aataccaaac caaatgtatt gacaaactta
    14881 tcggcaaaat agtaaatatc ttgaaagaac caagaaaatt cactttttaa aaacaatctt
    14941 gagaatacaa ttgttttttt cttttcaatc ataaaaaaga aacttaagat gaatgacata
    15001 gcaaacgcca ctgccatttt tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca
    15061 ttttgaagct gttcaaaaaa atcgtttcgc tcaatataag tatggatata ttgtaaaaca
    15121 ggatctgcat cttttggctg atgctcgtaa aaatcaataa cggactggat catatcaaat
    15181 gtttgatgaa taagcatcgg gatatacttt gtgaatgcca gatagataag gtaaacgatc
    15241 agtgtataaa tcatgatagc taaaacacct gtagggattc ggacaaatcg ctgaatgatt
    15301 tgaacaagat gaatcatcaa ataagtaaag ataaatgtga gcaaaatggt tgtaatcata
    15361 cttctaacta agaaaagaac aaaaacgaca aataataaaa cagtgagacg gcgtattttt
    15421 tcattcgcca aaaaacgctg ataaacggtc aaatcgacaa cctccaaata acaaaaaata
    15481 aacacgttat tcattatatg atggaatatg aaaataagaa agagaaagag caaaaagaaa
    15541 aaccgctaaa tgacgttatc ttatgagttg gttttcctca taaaatcgtt catctagcag
    15601 tttcagccct ttttctggtc ttgatgaaaa ttatctagca tttttcgggt cagatgttgc
    15661 agtgtatcca attcttcctg ttcaagtgga agataagact tgatcttttg tggaatacct
    15721 tctgcttgtt tttgcaacgc agcaccttga ggagtcagcg taacatttac gattctttca
    15781 tcacccgttt cacgctgacg attaatataa tttttttctg ctaacttttt tagtaaggga
    15841 gtcagcgtac cagaatccag acacaaatca tttcctagct ctttcactga agtctttttc
    15901 ttttcccata aaactaacat cacaagatac tgggtatagg taagattcag cggcttgagg
    15961 agggaagtgt agtgtctaat gatttcctta gaggcggcat ataaaggaaa acatacttga
    16021 ttaactaaat atagatcttc catttttcca cctccgctgt tttactatag tgtatcatag
    16081 cggatttaca caaaaaagtc tattcactaa ctgctttcag agtaacttct atatttccag
    16141 ctgtagcttt tgaatacgga cacactttat gcgctgcctc taataattct tgcgtttctt
    16201 ccagagaaag accttcgata gatccttcaa tctctacacc taagacaaag ctcgtttcac
    16261 tttctgaata aagggaaact tgtgcactga cagtagaagc cccagagata tttttttgct
    16321 gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa accagctgca aacaattgtt
    16381 cagggttggt tgcattttcg acgcgtttac caggagctgc aatattcaat tcgaatgtac
    16441 gatcgggatt atgaacttct ccagagcgtc cgcctgtgtt gatcattttc gtagaataca
    16501 tttttttcat aaaattacca cctttaatta tattttatac aattgaattt tacacaatat
    16561 aattgtggaa agcaaagaaa tggattggca taagataaat aagagaaagc acatgaaaca
    16621 gacgcgcttt ctcgaatagc aaacatatac aaaaaaatat cagagacaaa aactaatctc
    16681 atcataaaca ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa cttacttcac
    16741 gatggacctg atgaagcatc cataaataac caaaaggatc tctgaacatt gagttagtca
    16801 caccaaagtc catcatttct gtcatttctt gaatctctac acagccattt cgtaatgctt
    16861 tattatgtgt gtcctcgata tcagagacga tgatattgaa ccagatcgta tttggatgtt
    16921 cagcagtagg agcagtcaaa ccaagagacg tgttttcatc cagcatatgg aatctcgtgc
    16981 catacaaggt aaaaatgact tcattattgc ctctagggta atctgttgcc tcaatcagtt
    17041 gtatttcatc aaagattgtt tgatacagtt ctagtgcttt caaactatca tttaatacaa
    17101 aatctatttc cgttccttta tgcataaaga tctcctctcc ttttcagtcg aacagttact
    17161 agattctttt attataccag ttaaagaaaa cgcattcaaa aaaatagcgg atttacttac
    17221 caactcattc gattggatga taagtaaatc cgcgtcccat tacttctgct acgctgctga
    17281 caatgacaaa ggcacttgga tcgatttctt ctatcaatgg tttcattcgg gaaaattcat
    17341 gatcagtcac aacgatcaat aaaacctctt ttgactttcg attatacccg ccttttgcct
    17401 ctagcagggt aaggccacgt ccaatctcag cacttaagcg aaggcggatg tcttccaaat
    17461 aatgttcact catgaccata acagatttct ttcgattgat acctgtttcg atataagtca
    17521 tgacaattga agtgattgca atagataaaa tagcataaag aaactcttct attccaaaaa
    17581 caaacagatt aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca
    17641 agtggaaata ttttttaaaa atgaggggag ggatcgtcgt accgccgcta gaagagttgt
    17701 ttttatataa aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg acagacaaca
    17761 accgatctga agtaatcatt gtttctggaa ctattgcaat gattaaagga aaaaacaagc
    17821 ttccgaaaag tactttaaaa aagggttttt tacctaaaaa tagaagagct agtaccaaca
    17881 taacgatatt caacactaaa ataactatag ctcggtcaaa tcctaaaaca gcttccagta
    17941 atattcctag accgctgaca cctccagcag caatatgatg aggccctaaa aacatattga
    18001 tagcgactcc taatatgaag agggcaaaaa taatcttaat gtatgtccat ataaattgtc
    18061 t
//
LOCUS       DACDMM010000048        17975 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDMM010000048 DACDMM010000000
VERSION     DACDMM010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.048"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(29..727)
                     /locus_tag="IWD16_001239"
     CDS             complement(29..727)
                     /locus_tag="IWD16_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP9088315.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="IWD16_001240"
     CDS             complement(720..2072)
                     /locus_tag="IWD16_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP9088316.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="IWD16_001241"
     CDS             complement(2295..3695)
                     /locus_tag="IWD16_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP9088317.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="IWD16_001242"
     CDS             3856..4593
                     /locus_tag="IWD16_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP9088318.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="IWD16_001243"
     CDS             4718..5197
                     /locus_tag="IWD16_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP9088319.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="IWD16_001244"
     CDS             5194..5961
                     /locus_tag="IWD16_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088320.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="IWD16_001245"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="IWD16_001245"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9088321.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="IWD16_001246"
     CDS             complement(6864..7466)
                     /locus_tag="IWD16_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9088322.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..8041
                     /locus_tag="IWD16_001247"
                     /pseudo
     CDS             7572..8041
                     /locus_tag="IWD16_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8147..8731
                     /locus_tag="IWD16_001248"
     CDS             8147..8731
                     /locus_tag="IWD16_001248"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9088323.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="IWD16_001249"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="IWD16_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP9088324.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="IWD16_001250"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="IWD16_001250"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP9088325.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="IWD16_001251"
     CDS             10632..10811
                     /locus_tag="IWD16_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088326.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="IWD16_001252"
     CDS             10833..11858
                     /locus_tag="IWD16_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP9088327.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /locus_tag="IWD16_001253"
     CDS             12033..13277
                     /locus_tag="IWD16_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP9088328.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="IWD16_001254"
     CDS             13430..14377
                     /locus_tag="IWD16_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9088329.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="IWD16_001255"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="IWD16_001255"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP9088330.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="IWD16_001256"
     CDS             complement(15279..15521)
                     /locus_tag="IWD16_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088331.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="IWD16_001257"
     tRNA            15642..15714
                     /locus_tag="IWD16_001257"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="IWD16_001258"
     CDS             complement(15833..16471)
                     /locus_tag="IWD16_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9088332.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16594..17226)
                     /locus_tag="IWD16_001259"
     CDS             complement(16594..17226)
                     /locus_tag="IWD16_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9088333.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17577..17876
                     /locus_tag="IWD16_001260"
     CDS             17577..17876
                     /locus_tag="IWD16_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9088334.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6153 a         3150 c         3554 g         5118 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    16501 agatagagtc agtatacatg aagagttaaa ataggcaatg tttttttatg gatatttgta
    16561 ttgctaaaaa tttatcttat atattcattt tgattatact acttttattt gtaaaaattt
    16621 tctagcaaaa ccaagtgctt gcaactgatt aagcgtacaa ttttttagat gttccgagcg
    16681 tacttcaatc atttcaaagg agttgctact taaatcgata ttttttaaat cagtatcaaa
    16741 aaagctagca ttttctaaat cattagtaga aaacattatt ttagtgaggg aacagtctac
    16801 aaaatctgcg cttgataaga cagagtgctc aaaggtactt tcttttatgt ttgaaccaga
    16861 taaatttaca tagtccattt tacaattctc gaataacgtg ttcccaatat aactttccat
    16921 caatttagta cctgttaatt tacaattgac aaatttcacg cgatgcaaca tgctttttgt
    16981 caatatacag cctgaaaaat cacagttttc gaatatacag tcggtaatct ccattctttg
    17041 aaaatcattg gaagcaaaaa atgcatactt gaaaatagat ttgtaaataa ttggatggca
    17101 gattatttgg ttttcgatga cctctttttg tgaaaggata ttatacagtt caaaatcttc
    17161 gttttcatag atatgaaaaa aggatccttt attcaagctt gcttcattaa ctttaggaaa
    17221 aatcatattt aattacctcc attttcataa agtcatatta acaaaagcta aaccaactta
    17281 cattagcaga aaatgagata ggttttcgcc tttttcgata acgatagtgt cgctatctct
    17341 tgaaacgttc ttagaattgc cctgagagac acaaattagg cgtaattcta ataaaaatct
    17401 tagaataaaa atttttactg atttaattta gaatgttttt aaaataaaaa tcgttgccat
    17461 aacagtgtat tgaaaggttt taacaatcca gtaatgattt aaaaaaatct agatgtagcg
    17521 tatactttgt ttatagaatg tagtgggaag aaatatattt ttgggaggaa atcgtgatgt
    17581 ctaaaagtga agaaatagaa aacatggtac taaaattata tgatctcgtt ttaaatccag
    17641 agatcaaaga aaaggaacgt atacttttga tagacgcaaa gacaggctta gaaaaaggtc
    17701 agtattatcc taaagtcata aataatttgg aacgtagttt acgtccgtta gcaatcaggg
    17761 gagaattgtc taaaccagta tcaccgtttt atatggagat atcaacaata ggaaaatttg
    17821 aaaaagaatt aggtcgagga atggcttcgg caccgattac ttttgggcat ttataaaaac
    17881 gagttattca tctcaatgaa ttaaaaagta tagtcaatct cataatgcag gctaggttca
    17941 tttatttgaa cagagtctgt atttttttat tctaa
//
LOCUS       DACDMM010000049        17344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDMM010000049 DACDMM010000000
VERSION     DACDMM010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.049"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..368
                     /locus_tag="IWD16_001261"
                     /pseudo
     CDS             1..368
                     /locus_tag="IWD16_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            548..1489
                     /locus_tag="IWD16_001262"
     CDS             548..1489
                     /locus_tag="IWD16_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP9088335.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1558..2289
                     /locus_tag="IWD16_001263"
     CDS             1558..2289
                     /locus_tag="IWD16_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP9088336.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2433..4613
                     /locus_tag="IWD16_001264"
     CDS             2433..4613
                     /locus_tag="IWD16_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP9088337.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4633..5115
                     /locus_tag="IWD16_001265"
     CDS             4633..5115
                     /locus_tag="IWD16_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9088338.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5131..5892
                     /locus_tag="IWD16_001266"
     CDS             5131..5892
                     /locus_tag="IWD16_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9088339.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6023..7357
                     /locus_tag="IWD16_001267"
     CDS             6023..7357
                     /locus_tag="IWD16_001267"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP9088340.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7785..8270
                     /locus_tag="IWD16_001268"
     CDS             7785..8270
                     /locus_tag="IWD16_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP9088341.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8263..8946
                     /locus_tag="IWD16_001269"
     CDS             8263..8946
                     /locus_tag="IWD16_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP9088342.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9072..11717
                     /gene="polA"
                     /locus_tag="IWD16_001270"
     CDS             9072..11717
                     /gene="polA"
                     /locus_tag="IWD16_001270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP9088343.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11762..12598
                     /gene="mutM"
                     /locus_tag="IWD16_001271"
     CDS             11762..12598
                     /gene="mutM"
                     /locus_tag="IWD16_001271"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP9088344.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12562..13191
                     /locus_tag="IWD16_001272"
     CDS             12562..13191
                     /locus_tag="IWD16_001272"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP9088345.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13243..13575
                     /locus_tag="IWD16_001273"
     CDS             13243..13575
                     /locus_tag="IWD16_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9088346.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13887..14378
                     /gene="nrdR"
                     /locus_tag="IWD16_001274"
     CDS             13887..14378
                     /gene="nrdR"
                     /locus_tag="IWD16_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP9088347.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14401..15774
                     /locus_tag="IWD16_001275"
     CDS             14401..15774
                     /locus_tag="IWD16_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP9088348.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15774..16700
                     /gene="dnaI"
                     /locus_tag="IWD16_001276"
     CDS             15774..16700
                     /gene="dnaI"
                     /locus_tag="IWD16_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP9088349.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16829..17269
                     /locus_tag="IWD16_001277"
     CDS             16829..17269
                     /locus_tag="IWD16_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9088350.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5908 a         3032 c         3820 g         4584 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tcggaaaaag atcgtcaaga aatggatggg acgcttataa
      121 ttgaagtagg agttgaggac agtcaaacct catttctgtt atatgatcga gcatttgttg
      181 agtcccattc acctggcaca gctactagtg ccccatcttt gatgttttac agtaaagata
      241 tctttggact atataaaaaa atgcagacgc aatcagtcac attaggtgat atggtacagc
      301 ttggagaaga atatgtcttt aactttgctg atcccgatgg caactacttt gcggttacag
      361 gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa caggaaagcg aagtcttttt
      421 aattcaaata ataatattat gtaaacaaaa aactttccat gcgtaattat acacaagcaa
      481 ataaatcgtc aaatgcgaac gccttcttta tactgtttag gaacgaaaag aaggaaggaa
      541 gaaatacatg gattatcaag tactgcttta ttacaattat acaaaaatcg aagaaccaga
      601 ggaatttgcc aaaaatcatt tagccttttg caaatctttg gatcttaaag gtcgtattct
      661 agtagctgaa gaaggaataa atggtactgt gtctggaacc gttgaaaaca cgactgccta
      721 tatgaaagca atgaaagctg atcctcgatt tgctgatacg atgttcaaga tcgatccaag
      781 cgaaggacat gcctttaaga agatgtttgt aagataccgt ccagagcttg taagtttaaa
      841 gctagaagag gacattgatc caaaccaagt gacaggtgcc tatctttcac cgcaagaatt
      901 taaagaagct attttagatg aacagacagt ggtgattgat gcacggaatg attatgaata
      961 tgacttggga cattttcgag gggctgtacg cccagatata cgcagttttc gagagcttcc
     1021 agactggatc cgcgagcaca aagaagaatt tatggaaaaa cgggtagtga cctactgtac
     1081 tggtggcatt cgttgcgaga agttttctgg ttggcttgtc cgtgaaggat tcaaagatgt
     1141 cggacagctg catggcggta ttgctactta tggtaaggat cctgaagtcc aaggagaatt
     1201 atgggacggc aagatgtatg tgtttgatga acggatcgct gtcgatatca accaagtaga
     1261 taaacaaatc gtcggtcgcg actggtttga cggaacacca tgtgaacgat atatcaattg
     1321 tagcaatcct gagtgcaatc gtcagattct tgcttccatt gaaaatgaag aaaaacattt
     1381 aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat cgctatgttc aagaccatgg
     1441 aatctctcca gaagaagcag aactgcggtt tacagcttta attaaataga aaacaatagt
     1501 tttgattgtc gaccatttcc aatatgcgct atactagcgt tgaggatggt gatccacatg
     1561 aaatcattga tttatgctat tggggatgtc catggcaaac atgagctttt tgaaaaactt
     1621 attcactatt atgatccaaa gatccatcag cttgttttat taggggatct caatgatcga
     1681 ggtcctcgta caaaagaatg ttttttatta ggaaagcaat tagtcgaaga aacaggagcc
     1741 atttacctcc ggggaaacca tgaagaatat tttttgcaat ttctgcaatc accggaggac
     1801 tggtatgcgc cttatatgag aaatggtgga aaagaaacgt ttgaaagcct gattcaccca
     1861 ggggcatcgg aggaatactc cccaactgaa atctcgatga tgatccaatc gagatattca
     1921 gaactgatta aatttttagt ccagcgcccc ttgtattatg aatgggaaaa ttatcttttt
     1981 gtccatgccg gtgtggattt gacaaagaaa gactggcgta agacaagtct aagagacttt
     2041 atttggataa gggaaccatt tcatgaaggg aaaaacaata caggtaagac aattgtattt
     2101 ggacatacga ttacaccaat gctccatgga gatatgcaga caacagacct ctggattcag
     2161 gatcataaaa tcggaatgga cggtggggct gtattcggag ggtcattgca tggggttgtt
     2221 tttgacaagg aaggtattct tcaagatatt gaatatcaga acaacgaagg accttggcat
     2281 ggaatctgac taatggaaag aatgaaggag ctgtgacttt gttgcggctc tttttatttt
     2341 tatagagaat attgtttctt tcagtagttt aacccaggat aaaaagtatg atagaataaa
     2401 gaagcaattt ttagcagaaa agaggcattt taatgacgga aaatttgaac cagacgatca
     2461 ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa gcagttgact actgtactta
     2521 atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg ttaccgaaaa gaaatgactg
     2581 gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg gtatgcctat cttgaaaact
     2641 tagaaaaacg taaaactgaa gtgattcggc taatcgatga acaagggaaa ctgactcccg
     2701 agttagaagc agaaatcacc caagccgtga aaatgcaaca agtggaagat ttgtatcgtc
     2761 catacaaaca aaaacgacgg acaaaagcaa cgattgctaa agaaaaagga cttgaaccac
     2821 tcgcgaagtg gctgatgcag cttacagatg gagaggtgca atctgaagca gaaaaatata
     2881 ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt gcacggtgct catgagatta
     2941 ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg gattcggtct tatacgtata
     3001 acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc agatgaaaaa ggcgtatatg
     3061 aaatgtatta tgactttgca gaacccgtcc ataaaatggt ttctcatcga atattagcta
     3121 ctaatcgtgg agaaaaagaa gatattctga aagtattctt tcaagtagat gaagcagcaa
     3181 ttttagctta cttggatcgc caaatagtca aaaatcctgc ctctccttct tcttcattcg
     3241 tcagagaagc ctatcaggat agttataaac gctttattca gccagcaatc gagcgagaac
     3301 tgcgcaatga attgactgaa aaggcagatg agcaagcaat cgctattttc ggcgaaaacc
     3361 tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt tgtcctggga tttgatccgg
     3421 cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac agggaaagtt ttagcaatcg
     3481 aagttattta tcctcataaa cctgcagcgc aagcaaaaag agaagctgct ggaccagctt
     3541 ttatccaatt aattaataaa tatcaagtag atatggtagc tatcggaaat ggtacagcca
     3601 gtcgggaatc tgaacttttt gtggctgagc agttgaaatc cgccgaccat aaaacctatt
     3661 acgctatcgt caacgaagca ggcgcttctg tttactccgc tagcgaaatt gcacgaaaag
     3721 aatttccgca tctacaagtt gaagaaagaa gtgctgtttc tattgcccgt cgcctgcaag
     3781 atcctttagc tgaattagtc aaaatcgatc ctaaagctgt cggagtaggc cagtaccaac
     3841 atgacgtttc tcaaaaacgt ttggctgaac aattggattt tgttgtggaa acagcggtta
     3901 accaagtagg tgtggatgtg aatacagcaa gcccgcagct cttgcagcat atatcaggat
     3961 tgaataaaac aactgcccaa aacattgtca tttatcgtga agaaaacgga gaattcaccg
     4021 cacgaactca attgaaaaaa gtgccacgct taggaccaaa agcatatgaa caagcaatcg
     4081 gattcttgcg agtccctggt gggaaaaaca ttttggacaa cacaggtatc catcccgaaa
     4141 gttattctat tgcaaaagaa atattaactt cagtccaact ttcagaaaaa gagcttggta
     4201 cagaagaagc agtagaaaaa ttgacacgat tatccgtaga gaaactggct gaaagcctat
     4261 cagtcggaga agaaacgtta gccgatattt tagctgggct gacacaacct ggacgagata
     4321 tgcgtgatga gatgccagcg cccttattac gtacagatgt attgagtatg gaagacttga
     4381 aaccagggat ggaattgact ggaacggtcc gtaatgtcat tgacttcggc gcatttgttg
     4441 atatcggtgt gaagcaagac ggacttgtcc atatatcgaa actcagcaaa aaattcgtca
     4501 agcatcctac agatgtcgtg tcagtaggag atatcgtaac agtttggata gaacaagtcg
     4561 atacgaaaaa aggcagaatc agtttgacga tgctttcccc ttatgaagag taacaatgag
     4621 gagtaacaaa aattgacaca agaagaactg cagcagttag tagaagagat atcattaaaa
     4681 agcttccagc ttcctttcaa gcaccaagca atgttcaatc cacgcttacg tacaactggc
     4741 gggcgctatc atttaaacga tcatcattta gattttaatg cgaagctatt tgaagagtca
     4801 gacgaagaga cgattgcagg aatcatcaaa catgaacttt gccattatca tcttcatcta
     4861 gctggaagag ggtatcagca taaagatatg gatttcaagt atcttttgaa aaaaacagga
     4921 gggttgcgct atgcaccagc cttgacgaag aaacagataa agatggagta ttatcgatgc
     4981 caagattgtt ctgccacgat ctgccgcaag agaagaatcg atgtacgcaa atatcgttgt
     5041 ggaagatgtc atggagaaat caaatggatt gagacaaaaa gagaagactt taatgaagca
     5101 caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa gtgagcagag gaatctaaaa
     5161 gactggaatc agctattaaa ttttttagcc gcacctgaag agggaaattt tgaggaactg
     5221 acggcaccta cacttatttt agctggaaac tgtcttccta tcttagctga taaagcagca
     5281 caaatgtatt taaatggcca agtagatcaa ttgtttttgg tcggaggggt ggggcatgcg
     5341 actagaatcc tttatgagaa ttttgaaaag caaggatttc attttgaaga tggcatgagt
     5401 gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg atttgcccga taaggctttt
     5461 ctgatagaat cgaagtctac aaattctgga gagaacgcca ctttttcact ggagattctt
     5521 cattccttag gagctgtacc agaaaaaatc ttattgatga atgatccgac actgcaacgc
     5581 aggactcgag caacttttga gaaagtatgg caaaatgaac aaactatttt cgcgaatgct
     5641 gtgccatttg tgccggaaat ccttcacttt tctgaagaga tcatctttac agcaaaagaa
     5701 ctgaatcatc aatggcctaa ggaatatttc catgcgctag tattaggaga aatggaacgt
     5761 ctccatgacg atgagaacgg ctatggtccg aaaggaaaag attttattcc tcacattgat
     5821 atatcagagg aagtttggtc ttcttacacc agaattaaag agtcaataaa aacggatttt
     5881 tcccgtactt gaccatttta gcaagcgaat cctcctttcc aaataaattt ggttgaaatt
     5941 ttcaatcgtt tataagataa tgaggagcgt gttgaatgta atagctaaaa gaatggacgc
     6001 tagataagag aagaggattc aaatggaaaa tcaaaataaa ttgtatcatt ttgttggtat
     6061 caaaggttca ggaatgagct ccttggctct tgttttacat gaacaaggtc taaatgtcca
     6121 aggatcagac attgaaaaat atttctttac acaaagagat ttagaaaaag caaatatcac
     6181 gattctgcca tttaacgcag ataatgtaaa accagggatg acgatcattg caggaaatgc
     6241 atttcctgat tcacatgaag aaatccagcg agcaaaagaa ttaggtttgg aagtgattcg
     6301 ctatcacgac tttattggtc attttatcca aaattatacg agtatcgctg taacaggctc
     6361 tcacggaaaa acaagtacga ctggactgct ctctcatgta ttgagtggtg tccgtccaac
     6421 aagttatcta attggagatg gaacaggaca tggtgatccg caagcggaat tcttttcatt
     6481 tgaggcctgt gaatatcgcc gtcatttcct tgcctattca ccagactatg caatcatgac
     6541 caatatcgat tttgatcatc cggattatta tacaagtatc gacgatgtat ttacagcttt
     6601 ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg tatggtgacg atgcttattt
     6661 acgcaaatta aaagcaaatg tgccgattta ttattatggt gtcacagaaa acgatgatat
     6721 ccaagcacga aacatcgaac gcacaacttc tggttctgct tttgatgtct atcatgggga
     6781 tgaatttgtt gggcacttca ctgtaccagc attcggtaaa cacaatatct taaatgcttt
     6841 aggtgttatt gcagtagctt atttcgaaaa actggatctg aaagaagttg cagaagaaat
     6901 gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag atcgtagcag acatgaccgt
     6961 agtcgatgat tatgcgcatc accctgcaga gatcaaagca acgattgatg gcgcacgcca
     7021 aaaatatcca gataaagaaa taattgcagt tttccaaccc catacgttta cgcgtacgat
     7081 tgcattgatg gatgaatttg cagaagcact tgatttagct gataaagttt acttatgcga
     7141 tatttttggt tcggctcggg aagaacaagg aaacgttaag attgaagacc ttggagcgaa
     7201 aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta tctccattgt tagattatca
     7261 tgatgcagta gtaatcttca tgggtgctgg agatgttcaa aaatttgaac aagcgtatga
     7321 aaaactttta agcagcacga cgaaaaatgt actgtaatac aattactgca gtgtccttat
     7381 aaaaatagaa agagggggag tggcaaaaat cttgccaagc cccctttttt gcaacttatg
     7441 ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc caatcaaatc aaaaacatga
     7501 gaagctgttt ttcttggctg ttcttattac ttgatgcagg gcagcttttt cacgacctca
     7561 gataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaaat gaaaaatatg
     7621 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7681 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7741 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7801 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7861 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7921 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7981 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     8041 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     8101 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8161 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8221 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8281 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8341 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8401 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8461 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8521 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8581 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8641 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8701 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8761 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8821 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8881 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8941 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     9001 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     9061 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9121 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9181 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9241 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9301 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9361 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9421 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9481 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9541 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9601 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9661 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9721 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9781 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9841 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9901 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9961 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
    10021 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
    10081 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10141 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10201 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10261 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10321 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10381 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10441 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagataaa aaccaagaag
    10501 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10561 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10621 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10681 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10741 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10801 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10861 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10921 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10981 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    11041 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    11101 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11161 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11221 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11281 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11341 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11401 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11461 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11521 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11581 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11641 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11701 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11761 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11821 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11881 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11941 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    12001 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    12061 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12121 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12181 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12241 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12301 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12361 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12421 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12481 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12541 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12601 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12661 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12721 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12781 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12841 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12901 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12961 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    13021 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    13081 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13141 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13201 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13261 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13321 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13381 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13441 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13501 gaccacattt ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13561 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13621 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13681 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13741 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13801 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13861 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13921 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13981 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    14041 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    14101 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14161 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14221 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14281 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14341 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14401 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14461 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14521 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14581 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14641 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14701 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14761 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14821 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14881 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14941 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    15001 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    15061 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15121 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15181 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15241 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15301 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15361 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15421 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15481 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15541 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15601 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15661 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15721 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15781 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15841 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15901 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15961 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    16021 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    16081 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16141 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16201 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16261 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16321 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16381 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16441 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16501 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16561 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16621 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16681 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16741 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16801 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16861 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16921 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16981 atttgtcgga acgattgaaa gtattactca agtagaagca gaagaagaaa cgcttcagtt
    17041 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    17101 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17161 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17221 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17281 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17341 tggg
//
LOCUS       DACDMM010000050        17266 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDMM010000050 DACDMM010000000
VERSION     DACDMM010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.050"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..138
                     /locus_tag="IWD16_001278"
     CDS             <1..138
                     /locus_tag="IWD16_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP9088351.1"
                     /translation="ETFFRPMHNTQIRMAIVLFSIFLGYTASNFFLEVIALCRNLFVS
                     L"
     gene            235..1536
                     /gene="murA"
                     /locus_tag="IWD16_001279"
     CDS             235..1536
                     /gene="murA"
                     /locus_tag="IWD16_001279"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9088352.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1549..1725
                     /locus_tag="IWD16_001280"
     CDS             1549..1725
                     /locus_tag="IWD16_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9088353.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            1860..2156
                     /gene="yidD"
                     /locus_tag="IWD16_001281"
     CDS             1860..2156
                     /gene="yidD"
                     /locus_tag="IWD16_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP9088354.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2220..2687
                     /locus_tag="IWD16_001282"
     CDS             2220..2687
                     /locus_tag="IWD16_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9088355.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            2772..3656
                     /locus_tag="IWD16_001283"
     CDS             2772..3656
                     /locus_tag="IWD16_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9088356.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            3773..4006
                     /locus_tag="IWD16_001284"
     CDS             3773..4006
                     /locus_tag="IWD16_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP9088357.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4094..4174
                     /locus_tag="IWD16_001285"
     tRNA            4094..4174
                     /locus_tag="IWD16_001285"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4128..4130,aa:Tyr,seq:gta)
     gene            4181..4252
                     /locus_tag="IWD16_001286"
     tRNA            4181..4252
                     /locus_tag="IWD16_001286"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4213..4215,aa:Gln,seq:ttg)
     gene            4767..5474
                     /locus_tag="IWD16_001287"
     CDS             4767..5474
                     /locus_tag="IWD16_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9088358.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5534..6178)
                     /locus_tag="IWD16_001288"
     CDS             complement(5534..6178)
                     /locus_tag="IWD16_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP9088359.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6305..6592
                     /locus_tag="IWD16_001289"
     CDS             6305..6592
                     /locus_tag="IWD16_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088360.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6700..7020
                     /locus_tag="IWD16_001290"
     CDS             6700..7020
                     /locus_tag="IWD16_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9088361.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7101..8414
                     /locus_tag="IWD16_001291"
     CDS             7101..8414
                     /locus_tag="IWD16_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9088362.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8411..9130
                     /locus_tag="IWD16_001292"
     CDS             8411..9130
                     /locus_tag="IWD16_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP9088363.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9258..9809
                     /gene="raiA"
                     /locus_tag="IWD16_001293"
     CDS             9258..9809
                     /gene="raiA"
                     /locus_tag="IWD16_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP9088364.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10031..12565
                     /gene="secA"
                     /locus_tag="IWD16_001294"
     CDS             10031..12565
                     /gene="secA"
                     /locus_tag="IWD16_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP9088365.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12691..13792
                     /gene="prfB"
                     /locus_tag="IWD16_001295"
     CDS             join(12691..12753,12755..13792)
                     /gene="prfB"
                     /locus_tag="IWD16_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP9088366.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            13897..14583
                     /gene="ftsE"
                     /locus_tag="IWD16_001296"
     CDS             13897..14583
                     /gene="ftsE"
                     /locus_tag="IWD16_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP9088367.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14567..15460
                     /locus_tag="IWD16_001297"
     CDS             14567..15460
                     /locus_tag="IWD16_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9088368.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15612..17060
                     /gene="cls"
                     /locus_tag="IWD16_001298"
     CDS             15612..17060
                     /gene="cls"
                     /locus_tag="IWD16_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9088369.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         5795 a         2926 c         3759 g         4786 t
ORIGIN      
        1 gaaacttttt tcagaccaat gcataatacg caaattagaa tggcaatcgt acttttttca
       61 atttttttag ggtacactgc aagtaatttt ttcttagaag ttatcgcttt atgccgaaat
      121 cttttcgtta gcctgtaaat tttaaaaaaa ggtactattt taaaagcaag gatgttataa
      181 tagaaaagat tttgagtaag tgtatgctca ttggaatttt ttggagggaa caacatggaa
      241 gagatcatcg taagaggtgg caatcaatta aatggaacag taagaattga aggagctaaa
      301 aatgctgttt tgcctatttt agctgctagt ttgttagcag aagaaggtat tacgacatta
      361 gacaacgttc cgattctttc tgatgtcttc actatgaatc aagtgattcg tcacttaaac
      421 gtagatgtag attttgatga acaaaagaat caagtaacaa tcgatgcttc aagacaatta
      481 gaaatcgaag cgccttatga gtatgttagt caaatgcgtg cttcaatcgt tgtgatgggt
      541 ccgctacttg ccagaaatgg acatgcaaaa gtagctatgc ctggtggatg tgcgattggt
      601 aaacgaccaa tcgatttgca tttaaaagga tttcaagctt taggagcaaa aattattcaa
      661 aaaaacggct atatcgaagc aattgcggat gaattgatcg gtaacacgat ttatttagat
      721 ttccctagtg taggtgctac tcaaaatatc atgatggctg ccgtaaaagc aaaaggaaca
      781 accatcattg aaaacgtagc tcgcgaacca gaaatcgttg atttagcaaa cattttgaat
      841 aaaatgggcg ctcaagtcta tggagctggt actgaaacaa tgcgtattga aggcgtggat
      901 catttacatg cagtcaacca ctcaatcgta caagatcgta tcgaagcagg tacctttatg
      961 gttgctgctg caatgacaca aggcaatgtg ctgattgcag atgctatctc tgaacataac
     1021 cgcccattga tttctaaatt gatcgaaatg ggtgcagaaa tcattgaaga agaaggcgga
     1081 gtccgtgtca tcggaccaaa acatatctta cctacagatg taaaaacgat gcctcatcct
     1141 ggtttcccta cagatatgca agctcaaatg acagctatcc aattagttgc agaaggtaca
     1201 agtgtagtga ctgaaacagt gtttgaaaat cgtttccaac atttagaaga gatgcgccgt
     1261 atgaacgctc atgtcaaaat cgacggaaat gtagcaatca tggatggaaa ccatgaattg
     1321 caaggtgcag aagtatatgc aacagacttg cgggcagcag cagcattagt tttagctggc
     1381 ttgaaagcaa atggaatcac acgtgtgcgt aacttgaatt atctagaccg tggatattat
     1441 aatttccata tcaaactaca gcaattaggt gcagacgttg aacgtgtaga catggatcaa
     1501 acttctgctg aaaaaacagc acaaacgatt gcttaaggaa gtggctgaat gagtaaccaa
     1561 cgatatatct tattaacttt gataaagatt ttagtggtga tcttattgct tatccttctt
     1621 tttgtggccg gaacgatgat cggatatggt gtcatcggtg gcggcaatcc atttaaagta
     1681 ttccagccaa gtttgtggat tcatattcga gacttttttc attgataaat aagaatcaca
     1741 gtggttctag tcgatatgaa gtgagccatg aagcagaaca tgacaaaaga aaacagaagg
     1801 gaacgttgac gttagtcgcg atcccttttt ttagtaaaag aaaagaagga atctaattga
     1861 tgaagaagat attgatttat ttagttagag gatatcagcg ctttatttcg cctgcatttc
     1921 cacctagctg ccgttattac ccaacctgtt ccaattatat gataaaagca attcaaaccc
     1981 atggtgcact aaaagggacg gtaatgggga ctgcacggat cttacgctgt catccttttg
     2041 tcaaaggcgg tatcgattat gtaccattga cattccaatt aacgcgaaat aagaaagata
     2101 aaaaagaacc agattataaa aaggttgtga aaaatacgag tagcactgag cgataaagaa
     2161 aaagaggaaa attattgtca aagtaacaac tccgcaacta cttatatggg ggacaaaaaa
     2221 tgatcgaaga actaaacaag aaagatttac catggtcctt gttattagat gcagatcctg
     2281 ataaagaaaa agttcaagta tatgtttcca gagggagtgg ctggatatgg aaagaaaaag
     2341 aaaaaacaat aggtgtactg atctatgtgg caagggaaaa agaatacgaa atcgtcaatg
     2401 tagcagtagc cccaacgtat caaggaaagg ggattggcgg aaaattactt gagacagctt
     2461 ttcagaagct ttcacaactc gcttccagtc aaactcagat catcatacga accggcagta
     2521 ctagcagtgc ggcgttacat ctctatcaaa aaatgggcta tgtggagatt ggtcgagtca
     2581 aagactattt tatccatcat tactctgagc ccatttttga aaatggagat caattaaggg
     2641 atcaagtaat tttagcaatt cacttaccta aaaatagctt taactaataa actttcaatt
     2701 cgagaaaatg ctttcaaatg ctaaatagat acattacaat agctttagca aacaaaaagg
     2761 agaatatttt tatgggaata ttagtttttg attttggtgg atcggctgtc aaatatggtt
     2821 gttgggatgg caaagagatt aaaggtaagg gacaatttgc aacgcctgaa tcttgggaag
     2881 aaatgaaagc gcaattattt caagtttata aaaaaatact cgtttctttt gaagtcgaag
     2941 gaattggaat cagctctcct ggagtagtga ataatgagaa acaggtgatt gagggaatca
     3001 gtgcgattcc ttatattcat ggatttaata ttttccgaga tttggaaact ctgtttcaac
     3061 tcccagtaac gattgaaaat gacgccaact gtgccggaat ggctgaattt tatgaaggtg
     3121 ctgcccgtga ttatcaagat gttgcttttg ttgtggcagg aactgggatt ggcggggcgt
     3181 tgttcacgaa aggacaaatc aataaagggg cacatcttta tggtggagaa tttgggttga
     3241 tgttcttaaa tggcgataag acattcagta aacttggtac agcggtacag atggcttggc
     3301 gttattgtga acgcaaaggt ctcgataaaa attcttatac cggaaaagat gtctttgaat
     3361 tagcagaaac tggagatgca atagcaaaag aagaagttga gagtttttat acgtatctta
     3421 caaagggatt attcagtatt caattttcgt ttgatccaga agtgatcgta ttaggcggcg
     3481 gtgtttctgc aaaagaaggg ctcattgatg agatcaaaag ccgaatgaag aagctgacag
     3541 aacaattcga tctgcatgat ttcgaaccgc agattttatt atgtgaatac agaaacgatg
     3601 ctaatcttgt aggtgcagca gcaaactatc tcgctgtacg tcaaggggag aattaagagc
     3661 tttagagaag gtgcagcttg cataaaagaa gtgaatgaaa tgaataggag atttttacct
     3721 aaggaatgtt ttatttgctg cagtatgata aaatatctga gaggtgaaaa tgatgtcaaa
     3781 aaaaaataaa gatgttgaag tgcgtataga agaagtgaaa aaagatatca aaggtaagaa
     3841 ttatgaagtc actcagttat ttatagggaa aaaaatgatt ggtgagatct tagcctatgg
     3901 gccaaaagaa tatgaaattt tctttggtga agaagatttt ggaaaagaaa aaagtcttga
     3961 aaatgcgata gaaacagtga ttcgacactg gaatctgcac gaataaaaaa atatcaaaaa
     4021 aactttcaat aaagggttgc aaaaatctgg aaaattaggt attataacta agtcaggttg
     4081 atacaaacct ttcggaggag tagcgaagtg gctaaacgcg acggactgta aatccgttcc
     4141 ttagggttca gtggttcgaa tccactctcc tccatttcat tggggtatag ccaagcggta
     4201 aggcaaggga ctttgactcc ctcatgcgtt ggttcgaatc cagctacccc agtataccac
     4261 aatcgtaagc tctcgttagt tcgagagctc ttttttttgc tttgctacct tgttccttga
     4321 agattctgct gatttaagat agagttaagt taactacaag aattggaggg atccacatga
     4381 aatggaaaga aaaagtagac gaagcagctg aaaagttata tgatttggtc aaatcagaaa
     4441 aatacaatat tgaagtgaat attccgaaaa aaggcggaaa agcggtccag gtaaaatcaa
     4501 aacgcccaac gaatcataca aaaaaatgga tggctaaaaa ccgctaataa taacaaaaga
     4561 tccataagtc ggcattttca tcgattttag ggtcttttgt tattttctag gttgaacata
     4621 tctaaaaaat tgtttctgac aataggagta ggaagaacga tgtgtgtcta aatcctctgt
     4681 tgatggatag aaacctttat ctttttcagt tgtgaatcag gtatatacca gttataatag
     4741 aagaaacgaa aggacgggtg tacgttatgt cgaatcaatt acctgtatat atacagattc
     4801 atgaccaatt aaaaaatgaa atcgaacaag gtatatggaa agtcggagac cgtcttcctt
     4861 cagaaagaga attagcagtg aaattcaatg tcagccgtat gacgttgaga caagcaattc
     4921 aaaacttggc agatgaagga atcttagaaa gaaagatcgg ttcgggtaca tatgttgcgc
     4981 gagaaaaagt acaagaaaaa atgtctggtg caacaagttt tacagaaatt atggaatcgc
     5041 aaaatcgagt accgtctagt cggacaatct cttatttctt gacttcgcca agctcaagtg
     5101 aaatggaaaa actaaacttg agtaaagatg acacgatctt gcgaatggaa cgtattcgtt
     5161 tcgcagatga tgtccctatt tgttttgaag ttgccagtgt gccacaaaag ctgatcgatg
     5221 gctacagcaa agcagagatt actgaatcgt tgtatcgcac gctagaagaa aaaggcggga
     5281 aaaagatcgg tccagcgaat caaacgatta ctgctgtgtt ggcttctgag aaaattgctg
     5341 actatttaga ggtaaaacga ggagatgcag ttcttcgttt gcgccagatt tcttattttg
     5401 aagacggtac gccgtttgag tatgtgcgta cgcaatacgt aggaaatcgt tttgagtttt
     5461 atttggaaaa ataaaaaaca ggtgatccct gattccttac ttagaaataa aagagaatca
     5521 gaatcacctg tttttatata tgatcagtta ccagttgctc gtgataagaa acaggaccct
     5581 cttcaaattg gacttgtccg ttcagcagct caatgatgga ttctttaaag tggtcagcac
     5641 ttttttcatc tacagcacag atgacgctaa cttgatcagt atattggatt tcttttataa
     5701 aaaaatgttg gagttcaaga taattctgta attttcctag ttgcggatac gtgatcatca
     5761 cagagatctc ctgttgcaag gtaccttcta cgattccaat gtcagacaat gcttgagaga
     5821 cagcatgagt gtatgctcgt attaatccgc ctgtgccgag tttcgttcct ccaaaatatc
     5881 gagtgactac tgcagctaca ttgatcagtt gatttttttt gaggacttcc aacatcggta
     5941 cgccagccgt tccacttggt tctccatcat cgctgcttcg ctggatttca tttttttctc
     6001 caatgatgaa ggcgctgcag ttatggttcg ctttccaatg ttcttttttt atttcttgaa
     6061 tgaaactttt tgcttggtcc tctgattcta ttcgtctaag agaacagata aagcgtgatt
     6121 ttttgatctc aatctcactt tgtccatttt cttttattgt acgatagcgc tcgatcatct
     6181 tgtcacctct ttttttcatt ttctttattc agtataaata aagttctagg ttgtttcaac
     6241 actgtaacgg ttgcgcaatc gctgtagtat gcgataataa agacaaaggt aggtggatct
     6301 ggggatgcag gaacgaatga aaaagtacga tgcaattacg cattatttga agaacaatgg
     6361 cggctctcaa gtaacgttga cctttaccca atttgacgaa ctgctgtttc ccagtaacgg
     6421 acttcccaaa acagcaaggg aaagtacaga ttggtgggca aatgattata aacaccctga
     6481 aaaaggtgcc tatggctgga taaatgcagg ctatgaagta gttgtcatca atttggacaa
     6541 agaatatgtc gtgttcaata aattagtgaa atctagttgg ctatttgatt aaataaaccg
     6601 cgtttagaaa acgttgacaa atttctttct ttttatataa tagagtaaca gacgcgaaga
     6661 aaacgtttgc gtagtttgtt ttaaaaagga gagaaaagta tggctaaaaa aacaatcatg
     6721 cttgtatgtt cggctggtat gagcacaagt ttactagtaa caaaaatgca aaaagctgct
     6781 gaagcaaaag gaatcgactc ggatattttc gctgtgtccg cttctgacgc agataacaat
     6841 cttgcaaata aagatgtaga tgtactgtta ttaggacctc aagttcgctt tatgaaagct
     6901 gattttgaaa aacgtctgga accaaaaggc attcctttag acgtcatcaa tatggctgat
     6961 tatggtatga tgaatggcga aaaagtgtta gatcaagcaa tcacgctcat aggtgaataa
     7021 atagaacagt ataactatcg aacaagcgat ccaaaaaata gatcgcttgt tttttcgtat
     7081 atttttttag ggcgaataat atgaatcatt tttggggtag aaggcttctg acgagagata
     7141 ttaaagcaat agactgtgag gaggtaaatt taccaaatta tgagaagatc gcagcagtgg
     7201 aacgtttggg caactggatt ctttgtcatc gctgtggaat gaggacgtcc gtcattgatg
     7261 gacagcttgc agattacggt tatttttgta cacattgtct atatttaggg cgatgcgaca
     7321 ctcaacaaga tttatatttg tttgatcagc ctgaagcaaa acctcgagaa gtcgtctttt
     7381 tttggacagg acagcttact gaaaaacaaa cggaaatcgc aaagagaata ctttgccatt
     7441 cagaaaaaag acatcatctt atttgggctg taacaggtgc agggaaaaca gagatgcttt
     7501 atcctatttt agtggaaact ttaaaagctg gaggaagagt agcgatttgt acaccgcgag
     7561 tcgatgtttg taatgaattg tttttacgct atcgttatgt ttttccagaa gaaaaaatca
     7621 cgttgctcca tgggaataca gcaacggctt atacctttac ttcttttgtt atctgtacga
     7681 ttcatcagct ataccgtttt tatcggtcct ttgatttaat agtgatcgat gagatcgatg
     7741 cgtttcctta taacggggat gcaggattag ctcatgctgt gcaaacagcg attaaaccag
     7801 acggacgatt catctactta agtgccacac ctgataaaaa gttactaaag gaaatcaagc
     7861 agacgtttga catccacaaa cttgctttgc gctttcatcg gcgtttactt cctgaacccg
     7921 tcttggtttt catgaataat tggcgacaaa aatgcaagaa caagaagaag ataaaatctt
     7981 tggtgcggat cattcatcaa ttaattaaag aaaaccatat ccttttattt tgtccaagta
     8041 tcacgatgat gcatcgtttg aaagccacac ttcaagaggc tcttccgaga tatgagatca
     8101 cagatgtttc ttcaagagac aatcagcggg cggaaaaagt ccagaaaatg cgagaagcaa
     8161 aatatgacat actgttaacc acgatgatcc ttgaacgtgg agtcacattt gaaagaatat
     8221 ccgttatcgt tttaggagcg gatcatccgg tttttactaa atcttctttg gtacaaatcg
     8281 caggaagagc cgatcgtaaa gggccttata cgaacagtca agtatacttt ttctatgaag
     8341 aaaaaactgc agctattcga aaagcttgtc aggaaatcaa agaaatgaat gaagaaggga
     8401 agaagatgct gtgaggtgca gttgctgcag taaagatatc gtccaaaatt taacattcag
     8461 cgagattatt ggtgtttcga aatacagatt acctcagact tgttcagttt gtcgcgcaca
     8521 atttcattta ttggaaacaa gaggctgttc tggctgtcaa aaaccaatcc atttcaatct
     8581 agagagtacg aaaaagaaag taaagtctat tcccgtttac tgcagtgact gtcaagaatg
     8641 gaagaagaaa tatccgacct atgattttca gcaccgtgcc ttttttgtgt acgatgaaaa
     8701 tatgaaggcc tggctcaatc aatacaagtt tatgggagat atccgactag caggcacctt
     8761 tgcatataaa tggacgcaga taaaaaaaga atacccgaca tacatctatt gtccaatacc
     8821 tttatctgaa aaaaggcgaa cagaaagagg attcaatcaa gtaagcgaga tgttgaaagc
     8881 agcaagaatt ccttttcaga tgctactcaa aagagagaag catctgctgg ctcaagcaaa
     8941 aaacacgcga aaggaaagat tggagatgcc tcaacctttt gaacttgcag tcaccttttc
     9001 taagatacaa aataaaaaaa tattgattat tgatgatgtc tatacaacag gacagactct
     9061 tttccacgca gcagattgtt tgcaggtcgc acagccgaaa atcatccgaa ccatttcttt
     9121 agcaagatga attagatgtg gaaatttgat agcgatttaa tttgcaaata acctagagtt
     9181 cgatataata gaattaagaa gagggaaaga gtggtccttc tccttcttga ttgtggtaga
     9241 cgaaaggggt aatttttatg tttagatata atgtacgcgg cgaaaatatc gaagtaactg
     9301 aagcgattcg cgactacgtt gagaaaaaag taggcaaact agaacgctat ttcagtgacg
     9361 cacccgatgc aacggcgcat gtgaacttaa aagtttatac agaaaaaaca gcaaaagtgg
     9421 aagtaacgat cccattacct tatcttgtat tacgtgccga agaaacttct ccagatttat
     9481 acgcaagtat cgatttggtt gttgacaaat tggaacgcca aatccgcaaa ttcaaaacaa
     9541 aaattaatcg taaatcccgt gaaacgggca tgaatactgc agatgctact gctttattca
     9601 atgaagagga cgaaaacgaa aatgattctg aattagatat cgtacgtacg aaacgccttt
     9661 cattgaaacc aatggatagt gaagaagcag tcttacaaat gaacatgctg ggacataact
     9721 tctttatttt tgaagatgca gaaacaaatg gtacaagtat cgtttaccgc cgtaaagatg
     9781 gaaaatacgg actgattgaa acagactgat cagccttact taaataacaa cgaaaacttg
     9841 cagcagctac tgacagctgc cgcaagtttt tttgagtttc ctcctctttt agatgaaata
     9901 tgaataaatt atgaagttct tactttaata cgcttaagat atttcaatta tctataacta
     9961 atgataaaat gaaaagtaaa gtctacatag tctggataat acacagaagg aaaaggagat
    10021 tacacaacgc atggccaatt tgattcgttc acttatcgaa aacgataaga aagaactaaa
    10081 acgtttagat agcattgcaa aaaaagttga agcatacgct gaccaaatgg cagcattaac
    10141 tgatgaacaa ttacaggcaa aaactgctga attcaagggc agataccaaa aaggagaaac
    10201 attagatcaa ttacttccgg aagcttttgc agtcgtccga gaagcagcaa aacgtgtatt
    10261 ggggttatat ccacaccacg tacaattgat gggtggaatc gtgcttcacg atggaaatat
    10321 ccctgaaatg cgtactggtg aaggtaaaac cttgaccgca acgatgccag tatatctaaa
    10381 tgctctgact ggcgaagggg tacacgtagt taccgtcaat gaatatttag caacccgtga
    10441 ctcgacggaa atgggcgaac tttataattt cttaggatta acagtggggt tgaatatcaa
    10501 ctcaaaatct tcggatgaaa aacgcgaggc ttataattgt gatatcactt atagtacgaa
    10561 taatgaatta ggatttgact acttgagaga caatatggtc gtttaccgcc atcaaatggt
    10621 acaacgtccg ctgaattatg ccattgtcga tgaagttgac tcaatcttga ttgatgaagc
    10681 gagaacgccg ctgatcattt cagggcaagc agaaaaatcc acagctttat atacaagagc
    10741 agacaacttc gtgaaacgtt tgaaagaaga agaagattat aaaatcgatg tacaatcaaa
    10801 aacaatcagt ttaaccgaag cagggatcga aaaagcagaa gaaacattcg gtttggataa
    10861 cttatacgac attgaaaata ctgcattgac ccatcacttg gatcaagcat tgagagccaa
    10921 ctatatcatg cttttagata tcgattatgt tgtccaagac ggaaaagtac tgattgttga
    10981 ccagttcact ggtcggatca tggatggacg ccgttattca gatggacttc accaagcaat
    11041 cgaagctaaa gaaggcgtag agatcgaaga cgaaacgaaa accatggcga cgatcacttt
    11101 ccagaactat ttccgtatgt acaagaaact tgcgggaatg actggtacag ctaaaacaga
    11161 agaagaagaa ttcagagaaa tttataacat ccaagtatac cagattccga caaatcgtcc
    11221 ggttatccgt gatgatcgtc cagatttgct ttacccaaca ttacaaagca agttccacgc
    11281 tgtcgtgcaa gatatcaaag atcgctaccg caaaggtcaa cctgtgttag tcggaactgt
    11341 tgcggttgaa acatctgagt tattatcaga tatgctgaat caagaacgta ttcctcatga
    11401 agtgctgaat gcgaaaaatc actttaaaga agcagagatc atcatgaatg ctggtcaaaa
    11461 aggtgctgtg acgattgcta ccaacatggc tggtcgggga acagatatca agttaggact
    11521 tggggtaaga gaacttggtg gtctagcagt tatcgggaca gaacgacatg aatctcgtcg
    11581 tatcgataac cagcttcgag gacgtgcagg acgtcaagga gaccctggta tgtcacagtt
    11641 ctacttgtca ttggaagatg atttgatgaa acgattcggt tcagaacgaa tcaaatcttt
    11701 cttagatcgg atgaagatcg gtgaggaaga tgctgttatc caaagcaaga tgctgacgaa
    11761 acaagtagaa tctgcacaaa aacgtgtaga aggaaataac tatgacacac gtaaaaacgt
    11821 cttacaatac gatgatgtga tgcgtgaaca acgtgaagta atctataaac agcgacaaga
    11881 agtaatcatg gaagaaaaaa gcttatccaa gccactaatg aacatggtca aacggacaat
    11941 cagtcgtgta gtagatgcgc atacacaatt agagaaagaa aattggaatt tggaaagtat
    12001 catcgatttt gccggcagta ctcttgtcca tgaagatagt attactcttg cagatatcga
    12061 aggaaaaaca gctcaggaga tcaaagagta tctaaacgaa cgagcacaag aagtatttaa
    12121 taccaaagcg gctcaactaa atggtccaga gcaactgttg gaatttgaaa aagtcgtgat
    12181 cttgcgtgtt gtggactcta aatggacaga ccatattgat gcaatggatc aattacgtca
    12241 gtcaatcggc ttacgtgctt atggacagaa caatccatta gtcgaatatc aaacagaagg
    12301 ctataagatg tttgaagata tggtaggcgc catcgagtat gaagtcactc gaatctttat
    12361 gaaagctgag atccgtcaaa acgtacagcg tgaacaggtt tctcaaggaa cagcttctca
    12421 ctcagaagac ggagatgcca aagaaaatag taatactagc gcgaaaaaac agccaatcca
    12481 tgtgaacaag gtcggaagaa atgatccttg tccttgtgga agcgggaaaa aatataagaa
    12541 ctgtcatgga aaaaatgcaa actaatttct gacagcagga ttggtgagac aaattctttt
    12601 tttaagaatt cgtctcacct tctttctgtt atttgctgat tctgttagac tagatataat
    12661 gaaaaaagta tagtgaaaga aggaaaaaac atggaaatat ccgaaattcg aaatctcctg
    12721 acagaaatgc aggaaaaggt cacaagcttc aggaggtctc tttgacttag accagctgga
    12781 agaagacatt gcggaagcag aagcaaaaat ggctgaacct ggtttttggg atgatagcga
    12841 agctgctcaa aaagtgatca gtgaaaacaa tgcaaataaa gaaacttatg atcaattcca
    12901 ttctcttgct gaagaactgg aagaaatcga attgatggtg gagatgttgc aggaagaacc
    12961 tgatgcagcc atgcaagaag aagcagaaac acgtatcaat gcgctagatg aaaaaatgaa
    13021 gacatatgaa ttatctatgt tgttagatgg tccttatgat cgaaacaacg ccatcgttga
    13081 attgcatcca ggggctggcg gaacggagtc ccaagattgg ggcagtatgc ttttacgtat
    13141 gtatacacgc tgggcagagc agcatgggta tcaagtagag acactagatt atcaagcagg
    13201 tgatgaagca ggaatcaaga gtgtgaccct actgatcaaa ggacacaacg catttggtta
    13261 tctgaaatca gaaaaaggtg ttcatcgcct tgtacgtatc tcgccttttg actcagcgaa
    13321 acgacgtcat acttcttttt gttcggtaga tgtgatgcca gagcttgatg aaacgatcga
    13381 tatagaaatc aatccagatg atttgaaaat cgatacgtac cgagcaagtg gtgcaggtgg
    13441 tcagcacatc aataaaacag aatctgctgt acggatcact cacttgccta caggaacagt
    13501 agtagctagt caggcacaac gttcacaatt gaaaaaccgt gagcaagcga tggggatgtt
    13561 aaaagctaag ctttatcaat tagaagtgga aaagaaagaa caagaagcag ctgcacttcg
    13621 aggagaacaa aaagaaatcg gctggggttc acagatccgt tcttatgttt tccatccata
    13681 ttctatggta aaagaccatc ggacaaacgt agagacagga aatgtccaag cagttatgga
    13741 cggtgacctt gatttattta ttgacggcta tttaaaaatg catctacaat aagaacccat
    13801 tgttaaattg aaataaaatt gtaagatttt gcaagcatgt tgaaacattg ccatgctata
    13861 atagctaaag actgaaaaag atatggagag aacgccatga ttgaaatgaa ggatgtaatg
    13921 aagaagtact caaatggtac gaccgctatc cgcaatctct caattgtcat cgaacaagga
    13981 gagtttgtct atgtggttgg tccgtctgga gccggtaaat ctacattcat caaattgatg
    14041 tatcgagaag aaaaagcttc aaaaggaact ttgaacgtcg caggtcatga tctaatagca
    14101 atcaaaaacc gcgaagttcc ttatctacgc agagaaatcg gcgtggtgtt ccaagactat
    14161 aaattattac caagaaaaac agtatatgaa aatgttgcgt acgctatgca agttatcggc
    14221 agaaaacctc gggatatcaa aaaacgagta atggaagtgc tggaccttgt tggtttgaaa
    14281 cacaaagttc gagtctttcc tagcgaatta tccggtgggg aacagcaacg tgtctctatt
    14341 gctcgggcaa tagtgaatac gcctaaagta cttatcgcgg atgaaccgac aggaaatctg
    14401 gatccagaaa actcatggga aatcatgaaa ttattagatc ggatcaatgc ccaaggaact
    14461 actgtcgtga tggctacaca taacagtacg atcgtaaata cgattcggca ccgagtaatt
    14521 gctatcgaaa acggccgcat tattcgagat caagcggaag gggaatatgg atacgatgat
    14581 tagaaccttt ttccgccatt tattagaaag tatcaagagt ttgaaacgta acggctggat
    14641 gacagttgct tcagtcagtg cggtaacgat cacattgact ttagtcggtg tattcatggc
    14701 agttatcatg aacgcaacta aattagctca agatatagaa ggaaacgttg atgtctctgt
    14761 attcgtagat atcggtacaa aagacgaaga gatgaaaaag ctagaaaaag aattgaatga
    14821 cttagatcat gttaaaaaag ttgaattttc aagcaaagaa caggaattga aaaaaatcca
    14881 aaacgaaatg ggtgattcct ggaaattgtt tgaaggtgac aataacccgt tatatgatgt
    14941 gtatgtagta agtgctactg atccttctta tacaaaacaa gtagcagaag aagcagcaga
    15001 tttgaagaat gtttctcgtt ctgattacgg tggagcttcc tcagatcgga ttttccaaat
    15061 cgctggtgca gttcggacat ggggattagt tgcagcggca ttgctgctgt tcgtagcgat
    15121 gtttctcatt tcgaacacga ttcggatcac gattttgtct agacagcgtg aaattcaaat
    15181 catgcgttta gtcggtgcaa aaaatggata tattcgctgg ccgtttttcc ttgaaggtgg
    15241 ctggatcggt ctgatcggtg cgattcttcc gattttgatc atcacatttg gttatgcatg
    15301 gtttttccaa ttgatcaatc catcattatt acgttcccat tattcattga ttcatccaca
    15361 aaatatcgtt tggaaaatca atcttttaat ggtcggcatt ggtgtgatca ttggttcact
    15421 tggctcgatc atttctatga gacgtttctt gaaattctaa taccacaata taaataaaat
    15481 gagaaagttg gacagtttcg ttccagcttt ctttttttat tggctaggag aatagaagga
    15541 agcggctttt ttccctttac tttaaaaaac aacttgggta ttcttaagtt ggtagaagaa
    15601 gaggtgaagc cgtgaaaatt atcgttgaca atttttttac gatccttttg attatgaata
    15661 ttttgctttc ctttatcatc gtttttcgag aaagaaagga aactgctcaa acatgggcat
    15721 gggtgctcgt tttgatgttc attccagttg taggatttat tttatatatt tttttaggaa
    15781 gagggatttc caaagagaaa atctttgatt tgaagatcca agggaaaatc ggtaagaacc
    15841 tggaaattga agaagacaaa caggcattga tgcgagggct ttatcctcat ccgcctactg
    15901 gacaagtaga tgtgaaacaa ttgatctata tgctcacggt atttgaaagt acgctctata
    15961 cgactaaaaa tgaaatcact ttatttacag atggtcgtga aaaattcgat gcgctgattg
    16021 aagatatcaa gcaggcagaa gaccatatcc acttccagta ttatatttat cgcagtgatg
    16081 cattaggtga agaagttcga gatgcattga cagaggcggc aagacgaggg gtaaaagtca
    16141 gagtccttct tgatgcatgg ggttccacac aagtttcttc cagctttttt cagaatttga
    16201 aaaaagcagg cggagaagtc gcatttttct ttcctctatt tgttccgtat attaatcctc
    16261 ggatcaatta tcgcaatcat cgtaaaatcg tagttattga tgggaaaatc ggatatacag
    16321 gtgggttcaa tgttggaaat gaatatctag gattggtaaa gaaatttggt tattggcgag
    16381 acaatcattt gcgtatttac ggagaagcgg tgtatacctt gcaaaaccgt tttttgatgg
    16441 actggaattc tcagcataca aaagaagtag gctattctcc caaatttttt ccgtccattc
    16501 actctactgg tgatatagct gttcaaatcg tgacaagcgg accagatacc gaacatgaac
    16561 aaatcaaaat gacttatttg aaaatgattt cgttagccaa acgggagata ttgatccaaa
    16621 cgccttatta cataccggat ggatcgattc atgaagcact gaagttggct cttctttcag
    16681 gcgtaaaagt tcatatccaa atacctaaca aaccagatca tcttctcgtt tactgggcaa
    16741 cgtattcttt cgctgcagag ctaatcgagt atggagcaag gatcgaaaca tatgagaatg
    16801 gatttattca tgcaaagacg atgattatcg atggtggcat tgtttcagtt ggctcagcaa
    16861 atatcgatgt tcgttcattt cgcttggact ttgaggtcaa tacactggtt tatgatgaac
    16921 gaatggcttc acgtgtaaga aaagcttttt ttgaagactc gaaaatttct acgcatctca
    16981 caaaagaaat gtatgaaaac cgaggaataa tcatcaagat gaaagaagga ttagcaagat
    17041 tgatttcgcc gctcctttaa aacataaaaa ggacatgaga accatcaaca gatggagatc
    17101 tcgtgtcctt ttctaacggg gccatttctg agagtacgtc agaaatggag aaagcgtctc
    17161 gcatgagcga aattaacttt ggggtatctc ttgttttttg gggaaagcaa gaaataagct
    17221 aatatatgct attaaaagag acacatttca aagccagtat agcata
//
LOCUS       DACDMM010000051        16981 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDMM010000051 DACDMM010000000
VERSION     DACDMM010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.051"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            85..918
                     /locus_tag="IWD16_001299"
     CDS             85..918
                     /locus_tag="IWD16_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP9088370.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            934..1671
                     /locus_tag="IWD16_001300"
     CDS             934..1671
                     /locus_tag="IWD16_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP9088371.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(1826..2794)
                     /locus_tag="IWD16_001301"
     CDS             complement(1826..2794)
                     /locus_tag="IWD16_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP9088372.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            3026..3859
                     /locus_tag="IWD16_001302"
     CDS             3026..3859
                     /locus_tag="IWD16_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP9088373.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            3893..4732
                     /locus_tag="IWD16_001303"
     CDS             3893..4732
                     /locus_tag="IWD16_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9088374.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            4758..5318
                     /locus_tag="IWD16_001304"
     CDS             4758..5318
                     /locus_tag="IWD16_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9088375.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            5517..7238
                     /locus_tag="IWD16_001305"
     CDS             5517..7238
                     /locus_tag="IWD16_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP9088376.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            7403..8545
                     /locus_tag="IWD16_001306"
     CDS             7403..8545
                     /locus_tag="IWD16_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9088377.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            8561..9772
                     /gene="thiI"
                     /locus_tag="IWD16_001307"
     CDS             8561..9772
                     /gene="thiI"
                     /locus_tag="IWD16_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP9088378.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            10345..10992
                     /locus_tag="IWD16_001308"
     CDS             10345..10992
                     /locus_tag="IWD16_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9088379.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            11385..14030
                     /locus_tag="IWD16_001309"
     CDS             11385..14030
                     /locus_tag="IWD16_001309"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9088380.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            14340..15653
                     /locus_tag="IWD16_001310"
     CDS             14340..15653
                     /locus_tag="IWD16_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9088381.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            15643..16296
                     /locus_tag="IWD16_001311"
     CDS             15643..16296
                     /locus_tag="IWD16_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9088382.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            16351..16902
                     /gene="radC"
                     /locus_tag="IWD16_001312"
                     /pseudo
     CDS             16351..16902
                     /gene="radC"
                     /locus_tag="IWD16_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
BASE COUNT         5730 a         2868 c         3619 g         4764 t
ORIGIN      
        1 aaaggataag gattggaaat aatcctttta tcattaaaaa tatgatgacg aagtagaata
       61 aaattgggag taggggggaa agtcatggat atccaaactg ttttatctga tctgaaatta
      121 aatggtaaag tgattccagt tgttggtggc gatgttaatc agacctatcg tataaaaacc
      181 gaacatcgtg cttatttttt aaaaatccat ccgaatgtaa aaaaaggatt ttttgaagca
      241 gaagtagacg gattaaaaga actgtcagca ttcgtacgtg taccggatac gtatatgcta
      301 ggtgaaacaa gtgaaggcgc ttatttgtta atggaatgga tcgagccggg aaaaggggat
      361 caacgagatc ttgctgcagc tttagctaat ctccatcagc aaacagcgcc acaatttggt
      421 tttcgtaaag ataactattt gggtacactt gttcagaaaa attcttttga agaagattgg
      481 tggacttttt tctttaaaga tcggcttgaa tcccagattt ctttggcaga agaaacgaat
      541 cgatggaatg tacagcgtca agaaaagtat ctgcgattta aagaacgtgt attgaaatcg
      601 gttgaaccaa aaaagatcac tccacgcctc ttgcacggag acctgtggag tggtaatgtg
      661 ttctttgacc agcaagggca tccagttttt gtcgacccag ctgtttctta tgggaatcgg
      721 gaacaagata ttgcaatgag ccagttattc ggtggtttcc gaccggaatt tttagatgct
      781 tatcaaacta tatttccatt agaaaaggga tggaaagacc gactgccgat ttaccagtta
      841 tattatcttt tggcacattt gaatatgttt ggagaaagtt acggttccca agtagatcaa
      901 ttactagaaa atttttaaga ggagggcgtg tttttggcaa aaaaatatac caaaaagcat
      961 gaagtttcat attatgagtg tgacatcaac caaacgatga catttccttc gatgttagga
     1021 gtagttatca aaacgtctga agatcaaagc gaccagttgg gaagaggcag tgaattcgtc
     1081 aatagctttg gacttacttg ggtcatcacg aattattcca tggagattaa tcgtctccct
     1141 agagtcggtg aagtaatctc agtaacgact caagccatgg agtacaataa gtttttctgt
     1201 tatcggaact tctggatcca tgatgaagat gggaaagaat tagtcaaaat agaatctgtt
     1261 ttcgtattga tggattttgt gaatcgaaaa atgagcagtg ttaatgaaga gatcattgct
     1321 ccttttgaga gtgagaagat caagaaaatc aagcgtcagg aaaaaattga aaagattgat
     1381 tcgggcttca tgttgccata tcgtgttcgt ttttatgata tcgatagtaa tcagcatgtt
     1441 aataatgcga tgtattttaa ttggatcatt gacgtattag gatatgactt tttgactacg
     1501 catgttccag aaaaagtaat tattcgattt gataaagaag tagagtacgg gaatgagatc
     1561 gaaagccatt ttgaacaaat ccctatcgaa aatggtgtca aaacaaaaca tgaaatccga
     1621 atgcaagaac agctgtattg tgaagcaaat attatatgga aaaataaata aaaaagaggt
     1681 tgtgacagaa gggggcaact tcaagaaata agaagaaatt tacgaaaatt gtttctcaaa
     1741 tttttgtaaa ttttcgactt agttttcgaa gaagctattt ttgtcacagc ctctcttttt
     1801 aagtttagct attagataat gatgtttagt tttggataaa cgaaatcaat tcttcacccg
     1861 acaaaccata gtttacagca atcaatagtt ttaaacgtgc tttttgacta ttaatactgt
     1921 tacagaaaat aatccccatt ttttctagtt cttttccccc gccttcataa tcataaacag
     1981 gctctgcgat tccattgaaa caacgagaaa caagtactac agggattttt tggacaagaa
     2041 gcttttccaa agcagctaag gcttgtggag ggagattccc cgcacctaaa gcttcgatta
     2101 ccaaaccgtc tactttcgta tgggcgatgg cttccaataa gtccccttgc atcccagcat
     2161 atgctttaac gatcgggata gtcccatcta cagcggaaat gtccagtcgc tttgtttcta
     2221 aaagttcctg caagaataaa attcgattct tagttactaa gccgatggga cccaatgtag
     2281 gtgttcggaa agtagcaaca tttgtggtat gggtctttgt cacgtagcgt gcagaatgga
     2341 tctcatcgtt catgaccacc aaaaccccct tatctagcgc ttcttcacaa cttgcgacac
     2401 gaatcgcact ttcaaaatta taaaggccgt cacttccaag ttcattactt gaacgcattg
     2461 ccccagtcaa aacaattggt aatttgttgc caatcgttgt atcaagaaaa aaagcagttt
     2521 cttctaatgt atctgtaccg tgtgtgatca ccactccgga tatacctgat gcataagcgg
     2581 acaagatacg ttcttttaat accaacatgt gagaaggcat gatgtgtggt gagggaagat
     2641 tgaagatatc ttcaactaat aattctacat gcttggggat tgtgatttct gcatctaata
     2701 atgggtttgc cacatcagga ctaacaccgc ccgtgagcat gttttctttc atggcgatcg
     2761 ttccgccggt gtgtagtaca agtatttttt tcattttaca gacttcctta cgaataattt
     2821 tgcatagccg ttaaatctta ttgtatacta tataaaagag ttttgctatc agtgaagaat
     2881 aacgaatagc ctaacttatc ataaaaaagc tgaccttatt aaaatggaaa agactcaacc
     2941 ttgacctcct aatacaatgg agtatctggt tgagtttctt ttacaagaaa gcagccaagg
     3001 aaatcaatca cgaaggtggc cggaaatgat cagaatcgga ttaacatcgt ttaaagaaca
     3061 tgacaaatta actggtaaaa agaataacac tctttatgaa tatgcagggt ttctaccctt
     3121 agtggaatta gatactgcct attatggaat tccccgaaag tcctcagttg agaattggat
     3181 aagtgaagta cctgaatcat ttcgctttgt agtcaaagtc tatcaaggaa tcagcggtca
     3241 aggagactgg aagccttatt atcagaccga agaagagatg attgcgcatt ttttagatgt
     3301 tatgtcgcca atggtagatg ctggaaagat gttttgcttt ttagtccaat tcccctcatc
     3361 attcaaatgt acgaaagaaa atgtccagta cttgcgcaaa cttagaaagt ggtttgaggg
     3421 ttatcctgtt tctatagaat tgcgggatta ttcttggtac agcgatgaat ttcttgaacg
     3481 tacaagaaac tttatgtcta gagccgcttt ttctttagcc atcatcgatg agccacagtt
     3541 gctgaataca acgattcctt ttgatccatt tgtcaccaat ccgaactttg ctctttttcg
     3601 attccatggt aggaatgcgc aaggatggca agcaaatgat aaagagtggc gtaaaaaaag
     3661 aacgttatat cgttattcag aaaaagaact gcaagaacta gcacatgaag tcaaagtagt
     3721 tgattctaag acaaaagagg taggtgtgat ttttaataat aattctggtg gtgatgcggc
     3781 tgaaaatgca ttagcactgc aaaaaatatt aaatatcaag ccagatggac tcaatccaac
     3841 ccaaatggat ctgttttaag ggcttatttt attcaagaaa ggggcgaagt ctatgatcaa
     3901 agctattttt tttgatattg acggaacatt ggtcaccaat cgttcaaaag cgttggaatc
     3961 aacaaaacaa gcaattgaat atgctcggaa aaacggcatt ttatgcggtg tagcaacggg
     4021 gcgcagccca gtaaagatca aagaaatcat tgatgaattg gaattagata tgtatgtcgt
     4081 gtataatggt caactggttt ttactgcaga tcgcacgatt atcgaccacc cttttgaaca
     4141 aaaagtattg gaacatattg ttgaatttgc agatgagaac catcgacaaa tcgtttttgg
     4201 tgcgagaaat cggttagatg gatcaacgac gatgctatta ggacaatcga tttttatcaa
     4261 gcgactagtc agctttttac cgcgtaaatt cccggtacgt ctgatgaaaa aaatcctcca
     4321 ggtttttagc cctcaccgac aaaaggatcg ttatgaaacg ttagagatat taaaagaacc
     4381 aatttatcaa tgtatattgc tcagtccgga gtcagaacag aaaaagttag aacatcgctt
     4441 tcccgagtgt acgtttcagc gttcaaatac gtatactgta gatatcatcc caaaaggtgg
     4501 ttcaaaatta ctaggcatcc aagctttcgc aaatgctgtc gaaattgaaa tggaagaaat
     4561 tatggcattc ggcgatcatt acaatgatat cgagatgctg aaaggtgtag gaattggagt
     4621 ggccatgggg aatgctcaga tcgaagtaaa acatgctgca gactttgtga ctcaatcaaa
     4681 tgaagaagat gggatatacg aaggactgaa acacttcggt ctgatttatt agtcaatgaa
     4741 agaaggaaaa aatcagtatg gaagcagaga caccttttca aaaagtagaa acattccaca
     4801 aaacttttga tccaagaaga ccagagatac ccacggcttt tacagaaaat gaagctcata
     4861 cacgtgctgc gttcaaaata gaagaacttg ttgaatttgt tgcagctact tcacctaata
     4921 aagaacaact gcatgaagct gttatgctgc tgcacgaagc attagataaa gcagaagaaa
     4981 aaatacttgg taaaacacag tggggaagtg atccgctggt tgaacaagta gatgcactga
     5041 cagatatcct ttactttact tatggttctt tttcattact aggaatagtg cctgatcgaa
     5101 tattcgaaat cgtccatcag gcaaatatgg ggaaattatt tccagatgga aaacctcatt
     5161 atgaccctgt aacaaataaa gtgttgaaac ctgaaaactg gcagatggaa tatgctccag
     5221 aacctaaaat cgcggcagaa atccaacgtc agattcaaga agcgattgct aagaagaaat
     5281 ccgctgatat aggcatgtgc acaaatgaaa aagagtgatc agagtttcac tatttttgtt
     5341 atctttatac gatggaaaaa caaaagattt tcgtttaagt tagtgaatgc atgaaaatag
     5401 tatgctataa tctagaatag atgtcttttg cacgagagac gtattatttg gatttatgcc
     5461 aagcagaacg ccatctgttg cataaaagat gcatactgaa aacggaggaa ccataaatgg
     5521 gaaataatgt tatcatcgga atcgtcattg caatcattat agtcgccatt gtcgtctttt
     5581 tggttagtta cttgatgaag aaaaaaaacc aagatcgatt ggatgcctta gaaaaacgca
     5641 aggaagaatt atttgacttg ccagtgattg aagaagtcga tgatgttaaa aaaatgcatc
     5701 tggttgggca aagccaaaat acctttcgtg aatggagtca aaaatggaat gacatatcta
     5761 cttcctcgtt cgctgaacta gaaagtcaaa ttttcgaagt ggaaaacctc aatgaaagtt
     5821 ttcggttctt aaaaggaaaa aaagcagttg ttgaagcaga agctacaatg gaccaaatgg
     5881 agaaacaagt gacggaaatc cgtgaaggtc taaaggaatt gcgggaaacg gaagaacgca
     5941 actcactagc tgttcagcag gcacttgacg tctatgaaga actgaaagaa aaagtcagag
     6001 aagacgagca tgaatacgga ccagctcttt ttgaattgca aaaacagatc aaagtgatcg
     6061 aaaaagaatt tacgcagttt gtgactttaa acacttctgg agaccctgtt gaagcccgcg
     6121 aggttttgga taaagcagaa aaacatacgt atgaagtgga agctctgatg aaaaaaattc
     6181 caccgctgta tgaggagctc cataccactt tccctgaaca acttgaagaa atcagtgata
     6241 cttacgagaa attgatggaa gaacaatatg tattcccaga agaagatctt gctgaagata
     6301 tcgcaaaagt aagtcgaaga attgaaaatt cattagccaa tcttgaaaaa acagaagttg
     6361 aaacggtcga atttgaaaac cgtgaaacag ctgatttgat tgattcgtta tatgatatct
     6421 tagaacggga aatggaagct cagagatatg taaaaactaa ccaatcaacg attgcagagt
     6481 atatcaaaca tacaacaaag aataaccgtc aattattgat tgagctggat cacacatctc
     6541 aaagctatac attgaaccac aatgaaattg gccgtgtacg aggtttccaa accgaagtgg
     6601 aagaaatgga acgtcaaaat gaacagatga ttccacagat ccgtcaacac gaaattcctt
     6661 attcagaagt gcgcacgttc tataaaacag tcttcaaagt cttagaagat atcgaaaagc
     6721 aacaagtaga gatcgacgac tcattacatg aattgagaaa aggcgaaaaa gaagcgcaag
     6781 aaaagatcga tacattcgaa ttcaaattgc gcagtttgaa acgttttgta gaaaaacaac
     6841 gtttgcctgg cttgccgaat gattacctag aattcttttt tgttgctact gaccgtattg
     6901 aagaactaag tgttgtcttg aataaaattc gagtaaacat ggaagaggtc aatcgtttag
     6961 ttgctctatg tgaagaagat ctagaattat tagataagaa gacacatgat ttagtagatg
     7021 cagcagcatt aacggagcaa atgctccaat atgcaaatcg ttatcgtcat acacacgaag
     7081 aagtgagaga agcaattgat aaaagctact atttattcaa taaagaatat cactatcaag
     7141 aagcattaga cgaaatcggt acagcattag agcgagtaga acctggtgct ttcaaacgca
     7201 tcgaagattt ttattttaat catccagact tagtataaag caaaaaagtt agtaaaccag
     7261 caagagcaag acggattatt gtcttgttct tgtctttttt tgtatatttg aaaattgaaa
     7321 caagtgaaaa tttcacctgc tcttgaaagc accttggaca accgttataa tagaaaagaa
     7381 tttcaaaaaa gaggggtttc ttatgatata ttttgataac agtgcaacaa ccccaatctt
     7441 tccgcaaagc ttagatgcat atgtaaaaac cagtcagcgg attattggga atccatcaag
     7501 cttgcatgat ttaggcaatc aagctaaccg gttgttgcag caagcaagaa agcagatcgc
     7561 tgatttgatc catgtagaac cagaagaaat ctattttact tcaggcggaa cagaaggaga
     7621 taactgggta ttaaaaggaa cgatgatcga aaaaagagaa tatgggaatc atatgatcat
     7681 ttcaggagtc gaacatccgg ccgtcagcga gacagcagaa caattaaaag aattaggaat
     7741 cgaagtaagt atcgccccag tggataaacg cggatttgta cgtgtcgacg aattgaaaga
     7801 attaataaga aaagagacag tattagtctc tgttatggct gtgaataatg aagtaggatc
     7861 gattcagccc atccaggaaa tcagtgatct tttggatgat tttcctaaaa ttcatttcca
     7921 tgtagatgct gttcaggcga ttggaaaagt accattctcg caatggttga cagaaagagt
     7981 tgattttgca acattttcag ctcataaatt ccatggccca cggggaactg gtttcattta
     8041 ttggaaaaag ggtcgtcgtt tagcaccatt actgacaggt ggagggcaag aaagaaatca
     8101 gcgaagtggg acagaaaatg ttccaggtat tgtggctatg gttaaagctt tacgtttatc
     8161 attagagata aaggaacaaa aacctgagca gacacaacgc ttaaaagtag ctctaatcaa
     8221 tgcgttgaag acgtatgaga aagtgacgat tttttcagaa ggaactgact atgcaccgca
     8281 tattttatgt tttgctctaa aaggaattcg cggcgaagtt ctcgtccatg ccttagaaga
     8341 aaaacaaata tttgtttcta ctactagtgc ctgctcctca cgtaaacata tggcaagcag
     8401 tacattgcac gcaatgcatg taccaaatga tctggctaca tctgctgttc gaatcagctt
     8461 agatgaaaac aattcgcttg cagaagtcga gcaatttatg attattttca agcagcttta
     8521 taagaaattt tctgcaatca attaaaagag gagtgttctt gtgaaataca gtgaaattat
     8581 ggtccgttat ggggaacttt cgacaaaagg aaagaatcga aaaagcttta tcatgcaact
     8641 agcacaaaat gtcagacaag ctttgaaaga ctttccagaa atcaaaatcc atgctgatcg
     8701 ggaccggatg catcttttat tgaatggcgc agacgggcag ctagttattc caaaactagc
     8761 aaaaatattt gggatccaaa atttctctcc aagtattcgt gtagaaaaag atgtcgaagt
     8821 tttgaaacaa gctgttcaag acatcatgaa agaaatttat actgggaaag aaacatttaa
     8881 aattatcgct aaaagaagcg atcaccaatt tgaattgaca agtaatgaat tgaatcaaac
     8941 attaggtaat gctgtttttg atattttccc gcatatccaa gttcaaatgc gtcaaccaga
     9001 tattccatta cgtgtagaga ttcggcgtga tggcgcttat ctatcgtatg aaacgatcaa
     9061 gggagctgga ggattgcccg taggaacaag cggaaaagga atgcttatgc tttctggggg
     9121 gattgattct cctgtagcag ggtactttgc catgaagcgg ggagtggaaa ttgaagctgt
     9181 tcattttgct agtccgccgt atacaagtga acaagcactg caaaaagcaa aagatctaac
     9241 agccaaacta gcaccttatg tgggaacgat ccattttatc gaagtgccat ttaccgagat
     9301 tcaagaagaa atcaaaaagt cagtgccaca aggatattgg atgacaatta ctcgtcgaat
     9361 gatgttgcgt ttgaccgatg aaattcgccg tattcgtcat ggtttagtca ttttgaatgg
     9421 tgaatcatta ggtcaagtgg cttcacaaac tttgcaaagt atggtggcta tcaatgaagt
     9481 aacgaatacg ccaatcattc ggcctgtagc gacaatggat aaacttgaaa tcattgaagt
     9541 agcagaacag atcgatacat ttaatctttc tatccagcct tttgaagatt gctgtacgat
     9601 ttttgctcct ccacagccaa aaacacgccc tcaactggaa aaggtccatc aatatgaaga
     9661 gcgtttagca atagatgaaa tgatccaacg agcactagct ggcttgaaga tggaagagat
     9721 acaagcagaa tcgtctaatg aaaaaactga agaattttca gaattgctat aagaggttat
     9781 gaaaatagtg ttttgaccag agtattagag aaacaaaacg aaaaaatagc tccatgtatt
     9841 ttaccgtttt tgaactaatg tcgaagatta gctgattgaa taccatttat caagaagtta
     9901 tggaaaagcc ggttcttttt tactaactga aaaataaaat tttatttaga gatcaagcta
     9961 cagttgataa atgatataaa aaagagaatt tcccatgaaa tagcgaggtt gtgacagaag
    10021 tgggcagctt caagaaataa gaaggaattt acgaaaattg cttttcaaat ttttgtgaaa
    10081 tttcggctta tttttcgaag aagttacttc tggaacaccg tgtatcagag gttgcgaaaa
    10141 agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca tattttacgt
    10201 ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgttttattt gtctcacacc
    10261 cttttccttc ctacttgtaa ccttgctccc gtgttagaaa catgttacac taaatatgtg
    10321 aaattattaa caaggagcgt cgttatgcga gaaaaaacag atacactgcc aaaagcaact
    10381 gcgaaaagat tacctttata tttacgctac ttgaagatgc tggatgactc aggtataagt
    10441 agaatcaaat caaatgagtt cagcgaaatc acccaaattc cttctgcaac gatccgcaga
    10501 gacttctcgc aattaggcga gctaggcaga agcggatatg gctatgatgt cccttttctt
    10561 atcgatgtat tcaataatat ccttaatact aaagaagaaa aacgaatcgc attagtagga
    10621 tatggaaatc ttggcaaagc attgaaacac aacaatttta gaagaaatga aaatctaaat
    10681 atcgtgtgcg tctttgataa tgatccagca ctgatcaatc gagtaattga tggagaaatg
    10741 atctatccga ttgatcgttt cgcggagata gccaaagcaa aaaatgtgac agtagcaatc
    10801 tctacggtac caagtaaata ttcacaatct gctattgatg aaattgtcaa aggaaatgta
    10861 accgctatac ttaattttgc gcctgacaga gtaactgtcc cagcttatgt caatgttcaa
    10921 tacattgatt tgacaaccga gttgcagacg cttatttact ttgatgagaa ttactcagaa
    10981 gttttttctt aggaaaattt gagtaggctt attgatcttg cgagaactcg tcgtaattct
    11041 ttcaaaaatt ttttagtact aaatgaagtg gttttttcac gaccacttgt tgactaaaaa
    11101 aaaaaaccat tgtagaatga agcataacgc tgaacaagag gagtaactgg cacattcatt
    11161 cttagagaga gaactatctg ctgaaaggtt ctcaatgaca accagtgaag gtagcttggg
    11221 agtttctgtc ttgaatggag tagagataga cgagtcgtga tcgttaactc acttgagagg
    11281 caataatgat tgattattgc aatttaggtg gtaccgcgta aatcatacgt cctagaatac
    11341 gaaagtattt taggacgttt tttagtttaa aggaggaaaa cagcatgacg gaagaaaata
    11401 acctatcaac aaaatataat ccgcaagaag ttgaagctgg acgttatcag cattggttag
    11461 aacaagattt gtttaaaccg agcggagata aaaaagcaaa accttattca atcgtaatcc
    11521 cgcccccaaa tgttactgga aaacttcatt tagggcatgc ttgggatacg accttgcaag
    11581 atatgctgat tcgtcaaaag agaatgcaag gatatgatac tttatggctg cctggtatgg
    11641 accatgcagg aatcgcgaca caagcaaaag tagaagcgaa gcttagagag caagggattt
    11701 ctcgttatga tctaggtcgt gaaaagttca tcgatcaagt gtgggaatgg aaagaagagt
    11761 atgcgagcca catccgcgaa cagtgggcta aaatgggctt atctttagat tacagtaggg
    11821 aacgttttac attagatgat ggattatctg aagcagtacg taaagttttt gttaccttat
    11881 acgaaaaagg tttgatttat cgtggtgaat atatcatcaa ctgggatcct caagcacgaa
    11941 cagctctatc tgatatcgaa gttattcata aagatatcga aggcgccttt tatcatatga
    12001 gctatgaact agcagatgga tcaggcgtag tagaaattgc gacaacccgt ccagaaacga
    12061 tgttgggaga tacggcgatt gctgttcatc cagaagacga gcgttatcaa gcgctcatcg
    12121 gcaaaaaagt gattctacct ttagtcaata aacaaatccc aatcattgca gatgaatatg
    12181 tagatatgga attcgggaca ggtgtagtaa aaatcactcc agcacatgat ccaaacgact
    12241 ttgaggtagg taatcgtcat gatcttccac gcgtaaatgt tatggatgat gatggtacga
    12301 tgaatgaact agcaggaaaa tatgctggaa tggaacgttt tgctgctcgt aaagcaatcg
    12361 tgcaagatct gaaagaaatc ggtcgattga ttaaaatcga tacgatgatc cacagtgtgg
    12421 gtcattccga gcggacaggt gtcgttgttg aacctcggct gtctactcaa tggtttgtaa
    12481 aaatggcgcc gttagcgaaa aaagccattg ataaccaaga aacagatcaa gcagtggaat
    12541 tcttccctcc aagattcaat caaacgtttt tacgttggat ggaaaatgtc catgattggg
    12601 taatctcacg acaattatgg tggggacatc aaattcctgc atggtatcac aaagaaacag
    12661 gtgaaatgta tgtagggatg gaagcaccag cggatagtga aaactgggta caagatcctg
    12721 atgttttaga cacttggttt agttcagctt tgtggccttt ctcaacaatg ggctggccag
    12781 atacggaaag cgaagattat aagcgatact tccctactag cactttagta accggttatg
    12841 acatcatttt cttctgggta agcagaatga ttttccaaag cttggaattt actggcgaac
    12901 gtccatttga gaatgtcttg attcatggat tgattcgtga cgaacaaggg cggaagatga
    12961 gtaaatcatt aggtaatgga atcgatccga tggaagtaat cgaaaagtat ggagcagatg
    13021 ctctccgctg gttcttatcg aatggttctg caccaggaca agatgtccgc ttcagctatg
    13081 aaaaaatgga tgcttcttgg aactttatca acaaaatctg gaatgcttca cgtttcgtta
    13141 tcatgaatat cgaaggaatg accgtagaag agatcgattt atctggagaa aaatcagtgg
    13201 ctgaccggtg gatccttact cgtttgaatg aaacgatcga acgagtaact gagctatttg
    13261 atcgttttga atttggtgaa gcaggtcgac aactgtataa ctttatctgg gatgattttt
    13321 gtgactggta catcgaaatg agtaaagaag tactttatgg agatgatgct gtttcaaaac
    13381 aaaccacccg tagcatctta gtccatacac ttgatcagat cctacgttta ttgcatccaa
    13441 tcatgccatt cgttacagaa gaaatatggg aacatattcc tcatcaggga aattctctag
    13501 tagtggcaga atatcctgtt gtacgtccag agtttgatga tgaaacagct tctaaaggga
    13561 tggaagtatt gaaagaattg attcgagccg ttcgaaatat tcgttcagaa gtgaatacac
    13621 cattgtctaa accaattacg ttgctaatta aaacaaacga tgctaaaatc gatcaatttt
    13681 tagttgaaaa tacaagctat atcgaacgct tctgtaatcc cgaagagttg acgatatcaa
    13741 gtgagatcac tgcgccagat ctagcgatgt cagctgttct gacaggtgca gaaatcttct
    13801 tgccgttagc aggattgatc aatattgaag aagaaatcaa acgattagaa aaagaactgg
    13861 aaaagtggac aagcgaagta aaacgtgtcc aaggaaaact ttctaatgaa cgttttgttt
    13921 ctaacgcgcc ggaagaagta gtagaggcag agcgtgcaaa agaaaaagat tatttagaga
    13981 agcaaacggc tgtcaaagaa cgaatcgaaa gtttgcgtac tattcaataa gaatagaaaa
    14041 agagagtgtg aaatcagcta gctattacta aaagctagct gattgaacat cgttacagga
    14101 aagagtttga gcctcactct tagcttcgat tcataaggag atgtgacata aattttgtcg
    14161 catctctttt ttagtctact cttattatgt aggagaagga gggaggtgga tcatgtatca
    14221 agagtcaata aagtagttga taaaatcgtt cctctctctg tttcttttct ctttgtcata
    14281 cttttatttt tcttatgtgt tctatttgtt ataataaaag agaaatgggg gaattgttga
    14341 tgacgattga agaagcaata gaatggatcc atagtcgatt accatttggt tcacgtcctg
    14401 gccttgaccg agtcgaagct ttattagaag tggtagggca tccagagaga aaagtaaaaa
    14461 cgatccatat cgctggaaca aacggaaaag gttctactgt agcttatttg agaagcctat
    14521 tagaagaggc tgggctgaca gtaggtacat ttacttcacc ttatatcgaa acgttcaatg
    14581 aacggatcgc aatcaatggc aaaagcattc cagacaatga tttgatccag cttgtaaaaa
    14641 aatatcagcc actgatcaaa caacttgatc aatttgaagc agtagctggt attactgaat
    14701 tcgagacatt aacagcaatg gcatttgatt attttttgat gaagaaagtg gacgtagcga
    14761 ttatcgaagt tggcttaggc ggcttattgg attctaccaa tgtagctgta ccaatgctga
    14821 cgggaataac aacgattgga cttgatcata ccgaaatact tggagataca attgaagaaa
    14881 tcgctgcaca aaaagctgga atcatcaaac aaggcgttcc agtagtaaca ggaaatattg
    14941 ccccagaagc ttttactgtc atccaacaaa cagctaaagc aaaaaatgca ccgctttatg
    15001 tatacgggga aaattataaa ataaactatc tgcatcctga tgagatgtgg ggagaagtat
    15061 ttgaattttt taatgaaata ggaaagatcc ctcaattaac cactccgctt ttagggcaac
    15121 atcaagctga aaatgcagga gtagccatcg aattgtattt tgtttactgt caattggaaa
    15181 agctgccttt ccgagaaaaa gatgttttga agggattgaa gaaatctcga tggccaggaa
    15241 gaatggagcg tttgagtaac gagccattag ttgttttaga tggtgcccat aatacgcatg
    15301 caatgaaccg gcttgttgag aatttgaaaa aagaattcaa aggatatcaa atcaacattt
    15361 tgttttccgc cttacgaact aaaaatgtta gagaaatgct agaacgattg cagcaagtac
    15421 caaatgtcca tatttatttg acgacctttg aacatccaaa agccatcgaa ttaagtgaag
    15481 tatcagattt agcaagtgag agagtatcaa tcgtttcatt gtggcaattc ggattagcag
    15541 atatattgga aaagatgaca aatgaagacg tattagttgt gaccggttca ctttatttca
    15601 tttcagaagt tcgccattta ttaatacaat taggaggaga tcatgaagta taatgcagtg
    15661 atttttgaca tggatggttt gctgttcgac acagagttag tgtattacga agccacacaa
    15721 gtagtagcag acaagatggg ttttccttat gataaagacc tgtatttaaa attcttaggt
    15781 gtttcagatg aagaagtatg ggcgaattat catatgattt tctcggaatt tggcaaaaca
    15841 agtgtacaaa gattcataga tgattcatac caggaaacgc ttgaacgttt ttcgcaagga
    15901 gcggtacaaa tgaagcccgg tgtgttggaa ctcctctctt ttttagaaga acatcagatt
    15961 cccaaagttg tcgcttccag taaccaacga gcagtcattg aacttttatt gaagaagaac
    16021 aatctgtttg aacgatttga gacgattgtt tctgcagaaa atgttcaacg agcaaaacca
    16081 gacccagaaa ttttcttgtt agctaatcag aagctaggta cagcgaagaa agaaacattg
    16141 attttagaag attcacaaaa tggtatttta gcagcttctg cggctgagat cccggtagtc
    16201 atggtaccag atctgcttcc accatcccaa gaattgatca aaaaaacagc tggggttgtt
    16261 tcttctttac atgaaatccc tgcattgtta aaataaaaat ccatctcttt tcgtatctta
    16321 ttttagataa gatagaaagg agatgttttt ttgggaaaac gattgatcaa agaagtacct
    16381 actagctctt taccaaggga acgtatggaa atttatggcg cggaagcttt atcggatcag
    16441 gagcttttgg caatcttact gcgtaccggt caacaccctt atagtgtgat gtcgattgct
    16501 ggaaatctgt taaaaacgtt tggaggactg gcctctttac gacaggctac tttgcatgag
    16561 ttagaagaga ttcgagggat aggacgcgtg aaagcaatcg aaatcaaagc cttgatcgaa
    16621 ttaggtcgac gaattcagac ggatcatgaa agagcggctc caccggttcg taccagctac
    16681 gaattggccc agcaattgat tctggaaatg aaagatcaca aacaagaaca tttggtttgt
    16741 atttatttgg acacgaagaa tcaagtgatc ttaaaaaaaa cagtgttcat cggttcgctg
    16801 aatcagtcag tcgctcatcc tagagaaatt ttccattatg ctgtacgtta ttgtgcagca
    16861 aggatcgttt tagcgcataa tcatccttct ggtaatgttt taaccatagt ataataaata
    16921 atagtagtta aagtagttta agaggagata ttaaagggat attttacttt aaataaaaat
    16981 t
//
LOCUS       DACDMM010000052        16920 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDMM010000052 DACDMM010000000
VERSION     DACDMM010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.052"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(7..1236)
                     /gene="hflX"
                     /locus_tag="IWD16_001313"
     CDS             complement(7..1236)
                     /gene="hflX"
                     /locus_tag="IWD16_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP9088383.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1238..2155)
                     /gene="miaA"
                     /locus_tag="IWD16_001314"
     CDS             complement(1238..2155)
                     /gene="miaA"
                     /locus_tag="IWD16_001314"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP9088384.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2159..2896)
                     /locus_tag="IWD16_001315"
     CDS             complement(2159..2896)
                     /locus_tag="IWD16_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9088385.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2987..3514)
                     /locus_tag="IWD16_001316"
     CDS             complement(2987..3514)
                     /locus_tag="IWD16_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP9088386.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3694..4287
                     /gene="clpP"
                     /locus_tag="IWD16_001317"
     CDS             3694..4287
                     /gene="clpP"
                     /locus_tag="IWD16_001317"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9088387.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4391..4876)
                     /locus_tag="IWD16_001318"
     CDS             complement(4391..4876)
                     /locus_tag="IWD16_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9088388.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5029..5226)
                     /locus_tag="IWD16_001319"
     CDS             complement(5029..5226)
                     /locus_tag="IWD16_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088389.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5321..7081)
                     /gene="efrB"
                     /locus_tag="IWD16_001320"
     CDS             complement(5321..7081)
                     /gene="efrB"
                     /locus_tag="IWD16_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9088390.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7078..8808)
                     /gene="efrA"
                     /locus_tag="IWD16_001321"
     CDS             complement(7078..8808)
                     /gene="efrA"
                     /locus_tag="IWD16_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9088391.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9201..9413
                     /locus_tag="IWD16_001322"
     CDS             9201..9413
                     /locus_tag="IWD16_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9088392.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9415..11094
                     /locus_tag="IWD16_001323"
     CDS             9415..11094
                     /locus_tag="IWD16_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9088393.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11348..11548)
                     /locus_tag="IWD16_001324"
     CDS             complement(11348..11548)
                     /locus_tag="IWD16_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9088394.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11698..11841)
                     /locus_tag="IWD16_001325"
                     /pseudo
     CDS             complement(11698..11841)
                     /locus_tag="IWD16_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12076..12738)
                     /locus_tag="IWD16_001326"
     CDS             complement(12076..12738)
                     /locus_tag="IWD16_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9088395.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13102..13189
                     /locus_tag="IWD16_001327"
                     /pseudo
     CDS             13102..13189
                     /locus_tag="IWD16_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            13247..13978
                     /gene="erm(A)"
                     /locus_tag="IWD16_001328"
     CDS             13247..13978
                     /gene="erm(A)"
                     /locus_tag="IWD16_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP9088396.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(14104..14886)
                     /locus_tag="IWD16_001329"
     CDS             complement(14104..14886)
                     /locus_tag="IWD16_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP9088397.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(15040..15417)
                     /locus_tag="IWD16_001330"
     CDS             complement(15040..15417)
                     /locus_tag="IWD16_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9088398.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            complement(15424..16812)
                     /locus_tag="IWD16_001331"
     CDS             complement(15424..16812)
                     /locus_tag="IWD16_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9088399.1"
                     /translation="MEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKG
                     FRNTYFKQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEI
                     EAYLSELNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKP
                     RFIDEFVLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLK
                     YYQWKMKKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELN
                     KLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAH
                     IFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLP
                     VVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVK
                     ERLEQIIGSLTG"
BASE COUNT         5023 a         3519 c         2790 g         5588 t
ORIGIN      
        1 aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc ctttaacttc
       61 atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc ttctcaactc
      121 gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca ttagttccat
      181 cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct gagtagaaat
      241 caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt ctgccttatt
      301 gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt ttaagacagt
      361 ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca aaagatccat
      421 attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag gcaggtcttg
      481 gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac gccatctctt
      541 agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg cttgtgtcaa
      601 tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa tctgaaaaat
      661 ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt ctttcaattc
      721 tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta actttgtttc
      781 acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc cttgccctac
      841 taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc cttctttcga
      901 acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta ccggaagacc
      961 aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa agatgatcaa
     1021 atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt taccgataat
     1081 cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt ctcctgatgc
     1141 ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct ggtttgcttc
     1201 ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc agccattttt
     1261 tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca aaccactgtg
     1321 catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc gaatgtagtt
     1381 tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg aaaaattctt
     1441 tatatccaat accttgactg gcctggactt ctccaagctc atacacctta cgcgcttctg
     1501 ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca taaagaatac
     1561 tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc tcttctttag
     1621 gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc actttgcgtt
     1681 gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac tgattccata
     1741 atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc tcaggattcg
     1801 cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag agacccgtac
     1861 caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat tgtcgacctg
     1921 aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca atcaaatgat
     1981 gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc agatttttat
     2041 agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg agttccacac
     2101 ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt tccatatttt
     2161 acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca gttattattc
     2221 catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc gtccatgcac
     2281 gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa gtattttttg
     2341 gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta tcagaagtac
     2401 tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc aaagaatcca
     2461 aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat gtttcggcta
     2521 atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc attagcatcc
     2581 ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga actttagctg
     2641 ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct tccaatgttt
     2701 tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg tgcataacga
     2761 tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca cctactcgta
     2821 cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt ccgcgatggg
     2881 catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt aagaaaaaaa
     2941 gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag ctccaaacat
     3001 catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga gctttttttt
     3061 cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt ccgcctttat
     3121 aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt cgatgggaga
     3181 tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt tgcgctgaaa
     3241 atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat gcatcaaagc
     3301 tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc tttttattta
     3361 attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta cgctgttctt
     3421 tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca gcacctgtca
     3481 gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt gttttgtttc
     3541 tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt ttttatttga
     3601 ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat tatggtacac
     3661 tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac agtcattgaa
     3721 caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa agaccgtatc
     3781 atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc ccaattgctt
     3841 ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc tcctggtggt
     3901 tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc agatgtccaa
     3961 acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc tggtacaaaa
     4021 ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct tggcggcgct
     4081 caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac acgcgaacgc
     4141 ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga aaaagataca
     4201 gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat cgatgaagtc
     4261 atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt aagctctctt
     4321 atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt tattttatgg
     4381 atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga cctatctcga
     4441 cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa ggacggatca
     4501 ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct tctgttacta
     4561 atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat atcgtggtcc
     4621 ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac tgatgtccta
     4681 ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg attgtaccat
     4741 cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt aaaacagctt
     4801 gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg gcatttgatg
     4861 ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact aatcctattg
     4921 aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa aacctgctca
     4981 ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt attgtacatc
     5041 tatattttct tctattactt tttttaaaat taattcgtac acttctattt gtgctgccaa
     5101 gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct tcgtctgaca
     5161 gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat ttaaaaactg
     5221 ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc aagaatagag
     5281 aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct tcctcagcaa
     5341 attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc tcatgcgttc
     5401 ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca cggattgtcg
     5461 ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc attgcttttt
     5521 gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa atcaagattt
     5581 taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct aaagacacat
     5641 tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg ataaaatgat
     5701 ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca agctttccga
     5761 agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag accataccaa
     5821 atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca atccttattg
     5881 ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc ttgccggcac
     5941 cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc aaatcttgga
     6001 ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca tctcctaaaa
     6061 tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca tctaaaatct
     6121 cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat tgtgtcacgt
     6181 tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt tgaccgacaa
     6241 cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata taagtagcat
     6301 aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca cgaaacccaa
     6361 agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt tcgcgtccat
     6421 atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt agctcaccaa
     6481 gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt tttgagatcc
     6541 agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg atataaaaca
     6601 tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata aaagcttgtt
     6661 gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata tttccttgct
     6721 ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg tctcttctca
     6781 aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt aattgcgaaa
     6841 tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata taagaaaaat
     6901 tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt cctgtaacat
     6961 atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact aaaagaaaag
     7021 tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag gtttttttca
     7081 tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg ggcatttttc
     7141 caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata cgatgatctt
     7201 atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag attctgtcgt
     7261 ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg cagaaaagct
     7321 gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg ccaatcgttg
     7381 tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa atccatctgg
     7441 ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca tttcttcttg
     7501 cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa atagcagcgc
     7561 tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat ctctcacgtc
     7621 tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga tcaattggat
     7681 caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc ctggagaggc
     7741 tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag caaatgttac
     7801 attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg gatcttctac
     7861 aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag ctgctcttgg
     7921 atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga atatatactc
     7981 gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca ctgccccgct
     8041 ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt gtgcaacagc
     8101 catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta cagcaaaacg
     8161 tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg aaagattttc
     8221 ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata acggttcaga
     8281 caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta ctacatacaa
     8341 actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca tcattggtgc
     8401 cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag catcgtttgt
     8461 tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg aataactttg
     8521 gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa tcctcgaacc
     8581 aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga tcataaatat
     8641 cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga tcatacgtgc
     8701 taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata tgaaattgaa
     8761 aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt tctcatctcc
     8821 ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc ctattaatag
     8881 aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca ttgatttttt
     8941 ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata acaattaatt
     9001 gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga taagcaaaaa
     9061 cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga aaaaccaggt
     9121 ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca ctgctttacg
     9181 cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca aaagtacgca
     9241 accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat gtagacgctc
     9301 gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt ctagaaggaa
     9361 gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt taatatgaaa
     9421 ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg tgaaatcggg
     9481 aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc cgggatcaaa
     9541 ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag ctacatcata
     9601 caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga ccacatcggc
     9661 ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc tctcgcatta
     9721 gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga gctccatgag
     9781 atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt cagaacgacc
     9841 catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa tatcgtagcg
     9901 actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt gcataaaatg
     9961 gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa tgctgagatt
    10021 ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat cttttcaaaa
    10081 atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt gcagcaagca
    10141 gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag catggaaaat
    10201 gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac atttgtagat
    10261 gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac aggttctcaa
    10321 ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca aatccaaatc
    10381 cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac aacaagtgtc
    10441 aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg aaaaatcaat
    10501 aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct tcgtttgatg
    10561 aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt acatgcttct
    10621 ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa tggcgatgtt
    10681 ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg agatgtgtat
    10741 atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg tcacctgctt
    10801 tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa agaaatcttg
    10861 gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg agaaatgatc
    10921 aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg acctgactgt
    10981 tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt attcgaacgt
    11041 acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga ataaataaga
    11101 ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa acatgagaag
    11161 ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc tcttaaattt
    11221 atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt gtggcaggta
    11281 tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat ttgatttaca
    11341 gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat tcttcaacgt
    11401 cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg atagctgaga
    11461 agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct ttttcagcgt
    11521 tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa aagcactaat
    11581 ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa cttctattcc
    11641 caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga aagcaactta
    11701 cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc cgatgatcaa
    11761 gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt tggtaaaatt
    11821 catatcctgc tgcgatggaa tttcatatta tcaagagtaa attacaaaag aatcatttaa
    11881 atcaatactt tcactttgaa tacatgtatt tgaagtggaa ggtacttatt tcaaaaatag
    11941 taaaacctgt atcttaaatt acttaatagt aacataagat acaggctgat tttttattca
    12001 ttgttattta tactaaagca cccgatagct ctgaaaacaa tcacaaattc aacttttcaa
    12061 agccacagct ttaagttatt ttgtccagac aacccccatt gcccaaccca ttttatggaa
    12121 ttggcatcca ggcaacaact tttcatataa atcgtaataa ttttgttcag ataggtactt
    12181 atctgaagct aaatgctcaa gccatgattt agatgtgttg tgattataaa ttctaatcgc
    12241 attttttatt ccaaatttga aacaatgcgg taaaaactct tggattgccc ctaatttata
    12301 aacataggta ggcggtgtct cgacttcaga tacattatca agaataacta ttcttccttc
    12361 ctcattcaac agttccttca tttgctgtat tacgctggct atatcatcca aatgatgaaa
    12421 ggttgttcgg cttacaataa aatcaaactt ctcattaaaa ttaagttgtt ctgcattcat
    12481 attcagatag accgtatttg ttagttgacg tttagatttg gcaagatcga gcatttgatt
    12541 agaaatatca atccctacca cttcatcata ataacttgct aatttctcca ctaacaaacc
    12601 cgagccacat ccgatatcta atgctctgcc tttctttgga gacatattag acacaaagaa
    12661 tgaataatca ttcaaaagct cattcacgaa atcgtaatct tctgcaacct tatcaaactg
    12721 tgattctatt gtattcaaaa agatccccca ttcctacttt atcgacattc tttcattact
    12781 taccacttta gatgtttttt cgttggggat aaaacttccc tttagacaat tttatccaaa
    12841 gacaatacaa cagtgcaact ttattaaagt cactgtcctt tatcgcagcc tttacttttt
    12901 agtaaagaca gtggcttctc ttatcaagtt tcaaaacata ttattttgaa gaaaacgtcc
    12961 atctgaagtg tcaagtgcaa aattacatat aaaggtttat tctaaaatga aaagatgata
    13021 caatcatatt cagttacata aggaggtttc aattatgtgc accagtatcg cagtagtaga
    13081 aattacttta tctcattcat aatgaaaaaa atggaaagga gataaaagta tgggtacttt
    13141 ttctatattt gttattaata aagttcgtta tcaaccaaat caaaattaat tggttataat
    13201 gaacgcttaa tgtcagttca ttataaccag taaggagaag gttataatga accagaaaaa
    13261 ccctaaagac acgcaaaatt ttattacttc taaaaagcat gtaaaagaaa tattgaatca
    13321 cacgaatatc agtaaacaag acaacgtaat agaaatcgga tcaggaaaag gacattttac
    13381 caaagagcta gtcaaaatga gtcgatcagt tactgctata gaaattgatg gaggcttatg
    13441 tcaagtgact aaagaagcgg taaacccctc tgagaatata aaagtgattc aaacggatat
    13501 tctaaaattt tccttcccaa aacatataaa ctataagata tatggtaata ttccttataa
    13561 catcagtacg gatattgtca aaagaattac ctttgaaagt caggctaaat atagctatct
    13621 tatcgttgag aagggatttg cgaaaagatt gcaaaatctg caacgagctt tgggtttact
    13681 attaatggtg gagatggata taaaaatgct caaaaaagta ccaccactat attttcatcc
    13741 taagccaagt gtagactctg tattgattgt tcttgaacga catcaaccat tgatttcaaa
    13801 gaaggactac aaaaagtatc gatcttttgt ttataagtgg gtaaaccgtg aatatcgtgt
    13861 tcttttcact aaaaaccaat tccgacaggc tttgaagcat gcaaatgtca ctaatattaa
    13921 taaactatcg aaggaacaat ttctttctat tttcaatagt tacaaattgt ttcactaaat
    13981 taaagtaata aagcgttctc taatttcaca agaggacgct ttattcttcc caaaaattgt
    14041 tcaatattta tcaataaatc agtagtttta aaagtaagca cctgttattg caataaaatt
    14101 agcctaattg agagaagttt ctatagaatt tttcatatac ttaacgagtg ctttcacctt
    14161 tgaatatagt ccttcccact tatcatcaca ctctccccga tagccttttc tagctatatc
    14221 cagtaaagtt acatgctctt taggtaaaag aggtatagcc cattctgcag cgacatcttt
    14281 cgaggtaatt tcaccagtag tcactgtttg ccacattcga gctagggtta aaattacatt
    14341 acgctcatca ccttttatcc cctcaattag ttctggcaaa gaatccttaa ttgctcttcg
    14401 aatatctgtc aaaggtacgg agacaagtat acttgaagaa tcaggaccaa atagagaaat
    14461 actattcttt cttgcttgtg ctaaaacaat agccaaatca ggatcatagc ttggttcctg
    14521 aatttgtcca ttctcaaatt cacccctgag ccactcaccg tatataaatt ctctttttgg
    14581 aggatattgc caagggacaa cttcactcct atttataacc gtaacttcaa gtggtctaac
    14641 agaatccgta tttccaatct ttcctgatat agtcattagt ctttctgtta gtttttttcg
    14701 agttaattga ggtaaactat gattcacgac gactagaaca tctacatcgc tgttaatgcg
    14761 taaaccacca tttactgctg aaccaaatag atatactcca actattgaac ttccaaataa
    14821 atcttttacg atttttaatg tttgaatcgc ttgatttggt atttttccgt taatcaaatt
    14881 gctcatgatt tcacctcgta actgattatg ttcatataaa gtttatattg atactcaatt
    14941 tacttaccct agattggaca tatacctaaa tgactgttca ataaagctga ccgttagcgt
    15001 ttaagtacat cctttcacaa tttgtctaca gattaataat tattctttat tatacagatc
    15061 tccatataat ttttgaattt ggttctgtaa ttttttattt tctttttcta attccattac
    15121 tcttcttttt aaggttttaa taaggatttc ctccgaacga gaacttttct tgggttttga
    15181 gactacattt gctgttattt gacgctcacg aagggattcg attctttgcc taatatcgtg
    15241 ttccttataa agccatgatt tagaaacatt agcttccttt gctattgaat taaaattaat
    15301 aactttacct tcaatcgaaa atttagaaat cgctttgtct actttttccc ttgtcttttt
    15361 tgatttctgc ttcgccaaac gtacaatttc tgttgtattt ctaacttgtt tatccattga
    15421 taattacccc gtcaaacttc caatgatttg ttctaaacgc tctttaacac ggctattagt
    15481 ctctacttgt ctttgccatt gtttatcctt agctatggct aataactctt ctgtacgctc
    15541 taactgttct tcgtgctgtg gtaagaattg cttactggta cagaagtgag tgcaatctaa
    15601 gcatgcattc gcatgtggac aaccacctgc tactactggc aatctacaat aaccatttgg
    15661 aagcacttgt gcatttatat ttttcttgaa ccattgaagc tctacatcat cgacttcatt
    15721 atcttcatct agatcaagca catctccatt attggtaacc agtttttcct gaaatttagt
    15781 aaattcattt tttagagttt catcaaagat atgagcgtat ctgcttgtca tttctgggct
    15841 ttcatgcccc aaaaatttct gcacaatatg ctggggcatc ccgttgttaa tcattcttgt
    15901 tcctactgta tggcgaaagg catgggcatg gaatctataa atctcacctg atttatccac
    15961 tatattttgc tcataagcta atttatttaa ctcacctcta aatgtttctt gttttaatgg
    16021 cgatccatct tttcttggaa agaggtattc actatctgga aattcctctg aaactttatc
    16081 ttcccgaact ttaataagta aagctacctc tttagatatt ggaactatat gctccttttt
    16141 cattttccat tgataatact ttaaaaagaa atctccatct ttgtcctcta atagacagcc
    16201 ttttttcaag gtgcacaatt cacttatcct cattccacat tcttgaacaa tcatagtcat
    16261 cgtagctata tattcgggta atttatcaag atgactgttc aattgctcta ggacgaattc
    16321 atctataaag cgtggttttg ctcttggtat tttcggatag tcctcagaat aaattaatat
    16381 tttggaagga acatcatccc attctagcct aagaagggta ctaaatagtc cttccaatat
    16441 agagatcctc ccagttattg tactaggttt tattcccatc atgtttagtt cacttaaata
    16501 tgcttcaatt tccactctcg ttaattggtg tactctctga acttgtttaa atttcatgtc
    16561 cagaaaatta aagaactctt taagtctttg ggcaatatca cttacatagg aaaagctatc
    16621 catgttcaat ctcaacttac aatatctttt tacaagttgt ttaaaatatg tattccgaaa
    16681 ccctttaaag ttaattgtat attcatattg tgttgggtta accttatcat ctggcaaagg
    16741 taagttacgt ctatcccaaa cgtctttatc ccactcctct ccatcaaaat aaaagttctc
    16801 ataaaactcc ataaattgtt ttagattagt aacatagtag gaattagctt ttacaggtgt
    16861 tttttcttga ttagcagtaa tcttataatt agtagtggta attctaacac cccgttttgt
//
LOCUS       DACDMM010000053        16778 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDMM010000053 DACDMM010000000
VERSION     DACDMM010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.053"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..457
                     /locus_tag="IWD16_001332"
     CDS             <1..457
                     /locus_tag="IWD16_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9088400.1"
                     /translation="VGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRF
                     PKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPK
                     SMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(528..1838)
                     /locus_tag="IWD16_001333"
     CDS             complement(528..1838)
                     /locus_tag="IWD16_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9088401.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1850..3268)
                     /locus_tag="IWD16_001334"
     CDS             complement(1850..3268)
                     /locus_tag="IWD16_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9088402.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3508..5076)
                     /locus_tag="IWD16_001335"
     CDS             complement(3508..5076)
                     /locus_tag="IWD16_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP9088403.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5080..5361)
                     /locus_tag="IWD16_001336"
     CDS             complement(5080..5361)
                     /locus_tag="IWD16_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9088404.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(5358..6281)
                     /locus_tag="IWD16_001337"
     CDS             complement(5358..6281)
                     /locus_tag="IWD16_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP9088405.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6278..7156)
                     /locus_tag="IWD16_001338"
     CDS             complement(6278..7156)
                     /locus_tag="IWD16_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9088406.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7143..8480)
                     /locus_tag="IWD16_001339"
     CDS             complement(7143..8480)
                     /locus_tag="IWD16_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9088407.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8483..9586)
                     /locus_tag="IWD16_001340"
     CDS             complement(8483..9586)
                     /locus_tag="IWD16_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9088408.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9660..11168)
                     /locus_tag="IWD16_001341"
     CDS             complement(9660..11168)
                     /locus_tag="IWD16_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9088409.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11134..11826)
                     /locus_tag="IWD16_001342"
     CDS             complement(11134..11826)
                     /locus_tag="IWD16_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9088410.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11848..12822)
                     /locus_tag="IWD16_001343"
     CDS             complement(11848..12822)
                     /locus_tag="IWD16_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9088411.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13122..13994
                     /locus_tag="IWD16_001344"
     CDS             13122..13994
                     /locus_tag="IWD16_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9088412.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14576..16774)
                     /locus_tag="IWD16_001345"
     CDS             complement(14576..16774)
                     /locus_tag="IWD16_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9088413.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
BASE COUNT         4955 a         3522 c         2729 g         5572 t
ORIGIN      
        1 cgttggcatt catcataaaa gtgccgtcat tactttagtt gaaagattat ctaaagtcat
       61 tatcacgatt aaacccaacg gccgtaaggc attagatatt gaaactgccc ttaatcaatg
      121 gttttctcgc ttccctaaaa acttctttaa atctattacg tttgactgtg gaaaagaatt
      181 ttctaactgg aaagccatta gtaaccaaca tgatattgat atatattttg cggaccctgg
      241 aacaccttct caacgcccat taaacgagaa ttctaacggg attctgcgtc gtaatggact
      301 gccgaaatca atggatttta gaaaagtgaa tcagacattt atttccagtg tcagcaatca
      361 acgtaatcat attccaagaa aatcattgaa ttacagaaca ccaatcgaaa tatttttgag
      421 ctatgtacaa gaagcatttt attctagctt aatttgacaa atcatatatc attaaaaaaa
      481 ataaaggtca agctaaaata ttatttaagc ttgaccttat tataatttca acgattgtca
      541 aaagtattgt ttgcagctgt gttgccagtt tctatagata aatcacagct tttgaaacat
      601 aagttttgta ctgcttcgat tcgcccgaat tcttttgcag ttatttggca atgagaaaag
      661 cagacatctt ctataggctt ttcaggaaat gctactaaat caaaagctcg ggaaggaagt
      721 gagtaacctg gagaaaggca tgcctgtacg tttttgatgt gtatgtcttt gactttcgtc
      781 atttgctttg attcaggtat ttcttcagcc actgcttgcc aactttctgg catttgagca
      841 aggtcattgc tattttttct attgtattca ttatgccagt ccattatcca agaaaaagga
      901 aattgtacat caatcatatt cagattttct accctgatat tttcgatcac tccccctctt
      961 tcttttgaag atttcattcg aataccacag tctgactgaa aaaaattgat atcgtgaata
     1021 tatacgtcac taactccagc actaacttcg ctaccaatag ttacaccgta tcctgaacgg
     1081 attttacatc tagcaacctc gattcttgat gattttcgat tgactcttgc accattgcca
     1141 tctctgccag acttaatagc aatacaatca tcaccacagg ccaaatcaca ttcatagaca
     1201 cgcacatttg ttgaagaatc aatatcaatc ccatctgtac ttggtccatc attgttgcgg
     1261 atgattactt tttctactaa tacatcgtta gaataagtga tttgcaagtt ccaaaaacct
     1321 gatttctgaa aaatcaaatc acgaacaaca acatgttctg catgatacag taaacaagcg
     1381 cgtggacgtt taatagcata atccgcaatc caacgaagtc ccttttgatc gtattcagct
     1441 cttgtcccgc ttttttgatc ttttccccaa tacagctccc accaatggtc tccttgaccg
     1501 tcgattattc cttttccttc tataagaata tctttcgccg agagaacatt caaaatcgct
     1561 gctggccatt tcatttctac gccggctact cgtgtatcga tttctggaaa tgcttcgata
     1621 tctgttctcc ccttgaggac tgcaccttct tcaaagataa gatgactctt atcttttaaa
     1681 aaaagggctc cgactaaata aatgcctttt ggaataaata caggtaagtg ttctttcgca
     1741 ccttgatcga ttgctttttg cagtattatt gtatcatctt gttgagtgtc gtttgtttta
     1801 aagttcaaag cattgataat cccttttttt gtccgcaata cgtttctccc tatctttcat
     1861 tgattttttt caattcttca ccaataaata cagtttttga acgattcagt gggtaaccta
     1921 ctagcaaaat gattgcggat acaaataagc agaatgctgg taacaagttt gccatcgtat
     1981 aaatcctttc ttgtactaaa actgattgga tggctccacc tgttgtagac gattggtaac
     2041 caatgagtgc cagcataaat ccgcctaccg ctcctgcaca tgcttgtcct acttttctcg
     2101 caaaagaatt tactccatat actgttccat cttctcttaa gcctgtctta tattgatgat
     2161 aatcaatcac gtcagtaatg aatgcccaga ccatcaaatt gaataaacca gatcctaaag
     2221 tagcgataaa taaaagaatc agatataccc atgcatttgt tatatgaagg aaatacatca
     2281 gcaagtacat acatgaagaa gcaagtaacg caaatacgct tgcttccttt ttaccaaata
     2341 attttgtcag ctttgtagca aatggtgcta aaactactac tgttccgtaa gtaaataaaa
     2401 gagcaacaga cattgcttgt ttattatgga agtaatcatt gaataaataa gtcatattcg
     2461 tcccagctaa aatctgattg atcacaatga aaatgtcaac aatgactaaa ataatcagtg
     2521 ctcgattagc taacaaacct tttaccaact gagaagcaga tactttttcg tttttgacaa
     2581 ctcttactct ttcagtagtc aatttacaag ttaacatata tgcgacaaat gctattgcag
     2641 cacatccgat tgaaatccaa aagaaatgat tccctgaaac gacttgttgt ccactcttgt
     2701 tatgggtata cattaaaatc ggaatgaaga atcctgtagt tgatccgcca atcgcagaac
     2761 caatgcttcg gaacgtggac aacgaggtgc ggtcattagg atttgagcta attgctgctg
     2821 ccattgctcc atacgggata ttgattgttg agagaaaaat cccataaaca atgtatgaaa
     2881 caaaaatata agcaatcttc accgtcattg gaagatgttg gacgaatggc aaaaacaaaa
     2941 tgacagtcac taagcaaaat ggatacttca tttttctgat ccaaggaata aatcggccag
     3001 tttctgttaa ctctgagaca tcacataatc ttccaaccat cacatcagca aacgcatcca
     3061 aaattctcga aatcaagaat aacgtaccta cgattgcccc tgaaacgcca agaacattcg
     3121 tataataaat catcaagaaa ctattgacta aacctaaaat aaaacagtta cctagatcac
     3181 cgaacatgta gcctatctta tcggaaatcc caaattcctt tttttctttt tttactgtat
     3241 ttgctgtaac agcattttgt gaaagcattt gaattcctcc tacctcttct agtaaacgtt
     3301 ttcaaaatta taactctttt tttcttgttt ttatattcac aatgttggct ttttattgca
     3361 ttatcttgac agataaaaaa caaaaaataa tctaaaaaca ttgatgcata agattgataa
     3421 ctttttttac ataacaatat atacaaacaa aaaaattcac taactagtga aaataagtta
     3481 gtgaacctca tttattaaag aattaaatta cagatgatct tttccatcat acttgaaaaa
     3541 ctcaaaatca gcataacttc ctcttttttt atttagatca tgcactgcta tcccgataaa
     3601 atttccagta aaccctcctg agagtataca catgtttttt tgcggaccta ctttcttcca
     3661 ttcttcagtt gatacgtctt tcaaatgaaa ctgtgctgtc tctttcttta cagtgacttt
     3721 caaaaaagtc ttcttttctg tgagtggaat cgtccaatct tctatttgtg ctactccgtt
     3781 ttctgattct atcatacgta ggcattttcc cttgtcctca ttccaagtaa cataacaata
     3841 aatataattg gattcattta agtacagtac cagtccagcc atctgattaa aattattagg
     3901 catgtattca aatgctgtct ccgcatcaaa agtaaaatcc gtttgccgaa tagctaacaa
     3961 atgatgttcg aaggtagatt gaagagattc accggaaatc agacgtaaaa aacctggtct
     4021 acattttgta tcacaccaat cagcatttgg caaaatgcgt ctggcattcc agttcttctt
     4081 tagctctcct tcaaatgtat ccataaactg atgatctttt tcttgttgta cctcttcatt
     4141 tgtctcaatc attatttctt ccatcggagt tgtctggcta tctttaagtc ttaaccaatc
     4201 gtcggacgtc caatacactt cttgaatagc ggtttctctt cccaaaatag ccgcaccgtt
     4261 taatggtctg gtacaaagat atgtcatgta ccatcttcct gacggggttt gtactaagct
     4321 cccatgtcct gtacattgca atgtagaagc aggtttgtca ctagctgtca gcataggaaa
     4381 accaggatca agctcgtaag gaccagtaat attctttgct cggcaaactg taactgaatg
     4441 tcctcttcct gtaccgcctt ctgccgtcat caaataataa tacccgttac gaaaatacaa
     4501 atgtggtgcc tccgttttcg ctaattctgt acctgcaaat attttataga tgggaccatc
     4561 aagcttcttc ttctctgcat catattcttg taatacgata ccgctggatt tattcggtgt
     4621 gtccagacga taatcccata gttcattgac aagccatttt tttcccgttt caagatcatg
     4681 gaacaaagaa ggatcaaagc cgttactatt taaataaatt ggttcggacc aaggacctgt
     4741 gatctcagat gcagtgatca aataattgtg gacatcttta aacggacggc ctgcactttt
     4801 gacatctgtg taaacaagat aaaacaatcc atcagcataa ctgatctgtg gcgcccatat
     4861 acttccattt gtaggatttc cttgcaaaga tacttgagtg gtcaaaatat ccgttgtatg
     4921 tgaccagttg actaaatttt tcgactcata tactcggatt cctggaagcc attcaaaaga
     4981 ggagacaata atgtaatacc gctctttcac tcttaatata ttaggatctg gattgaatcc
     5041 tcttaaaata ggatttttca ctttgatagt ctgcatgatt caggctcctt ttctcattct
     5101 tactcgatat aaactgaatg ccccagctgc taaaaaagca agtgaaataa acaacacaac
     5161 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     5221 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5281 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5341 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5401 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5461 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5521 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5581 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5641 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5701 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5761 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5821 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5881 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5941 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     6001 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     6061 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     6121 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     6181 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6241 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6301 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6361 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6421 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6481 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6541 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tgtttcgatg tccacctgtg
     6601 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6661 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6721 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6781 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6841 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6901 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6961 gagaatgcag gtaaaggacc gtcggaaacg gtccttcttt ttctggataa gcatagaggg
     7021 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     7081 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     7141 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     7201 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7261 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7321 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7381 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7441 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7501 atccttctga atgctgatgt ccgttcatct atcggataag cctttttttc ccatacatac
     7561 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7621 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7681 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7741 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7801 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7861 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7921 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7981 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     8041 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     8101 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     8161 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     8221 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8281 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8341 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8401 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8461 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8521 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8581 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8641 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8701 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8761 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8821 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8881 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8941 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     9001 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     9061 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     9121 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     9181 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9241 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9301 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9361 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9421 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9481 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9541 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9601 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9661 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9721 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9781 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9841 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9901 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9961 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
    10021 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
    10081 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
    10141 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
    10201 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10261 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10321 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10381 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10441 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10501 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10561 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10621 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10681 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10741 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10801 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10861 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10921 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10981 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    11041 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    11101 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    11161 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    11221 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11281 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11341 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11401 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11461 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11521 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11581 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11641 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11701 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11761 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11821 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11881 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11941 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    12001 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    12061 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    12121 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    12181 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12241 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12301 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12361 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12421 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12481 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12541 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12601 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12661 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12721 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12781 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12841 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12901 tttttatcgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12961 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    13021 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    13081 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    13141 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    13201 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13261 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13321 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13381 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13441 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13501 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13561 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13621 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13681 ttatgctgcc attagcttta ccgtctattg ctactatctc gcttttttat gctgttactt
    13741 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13801 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13861 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13921 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13981 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    14041 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    14101 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    14161 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    14221 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14281 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14341 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14401 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttaaacggcg
    14461 tttatcttgt tgattctgcg gcaataaaat cagccagcag caaaacatga agtgctgttt
    14521 tttgctgtcc tcttttattg cttgaatcag atcacaagaa aaacagcctc ttgattcaca
    14581 aatatttttt tcgatattct cccggcgtaa tcgattcctt ctttttaaag aaacgaataa
    14641 aattttgcgg atttcgatat tgcaatcttt ctgcaatctc ttttacggag aggttagtct
    14701 ctttcaacca tttttttgct acctctaaac gatgattttg aacaaaatca ccaaattttt
    14761 ctcctgtttc ctttttaaat acattactta aatagttagg gttataatgc aacctatcag
    14821 cgatcacatc taaagacaga tcacgatcat attctgtgta gacgatatgc ataatattct
    14881 ccgacaaatt tttaaattca tgatcagtga ttgcttgaga gcattctgtg attggcagaa
    14941 ctaattgttc taaaacaagt ttttctagtt ctttgggatg ataattgttc agtgctttta
    15001 cataaatttg tttcatgtta tcaaagtttt ttgaatctac tcccaataac tgtcctaatt
    15061 gaacgagttc attgatcaag cggaccgaaa ctacttcccg actcaaagga tttttatttt
    15121 gtgcaaacaa tgcatcaatc aattcatgta ctaaattctt cacttcctga tttccagaac
    15181 gaatggcttc aaacaatcga ttttgttctt ctatcggata tctaataagt gttttttcat
    15241 gagcagcagg aacgatttcc tgataaaaaa taattgaact tggaccagta tttacacgat
    15301 aatacagggc ttcttttgcc cgatcaaccg attgcttgct ttcattcagc gtttcgaacc
    15361 ggttactgat gcctatactg atagttaaat tcaagtattc ttttacacgt ttttgaatga
    15421 gtcggcaata ctccatgact tttttgcttg cttctgcatc atctttatcc attcgataaa
    15481 tagtggcttg catttcttca ttcaaaacga tcggcagcat tcgattttcc tttggcacaa
    15541 tttccgtgat catgttgttg atcgctaaaa gtagcaaatc acgctcccca ccgtgactat
    15601 cttctaacag atcgatttga accaaagctg tataatagca gtcattttga gaactataac
    15661 caaattgctg caaccgctga tctagttctc gcctttctac tcgatttcta aataaactaa
    15721 gtacaaatag cgtttccagc tgtggctttt gggtaactaa gtgtgcactt aacgcttctt
    15781 tttctcctat gatccgactg actgattgag cgaccaaatc tagttctttc ccactgaatc
    15841 cttttgattt aagattcaga cgttcctgta tttgagaaat cggacgggca aatctgtccg
    15901 aaaataagta ggaaaaaaga ccaatgagtc ctaccagtgt taaagacact gcaactagcc
    15961 cgattctcag attgcggatc attgcgccaa ttgcactaga gtcaacttct aatctgtaaa
    16021 tccaattatt ataatctgat ttcagtacta tatacgtttt atctttttct cttatcactt
    16081 tcagctgatt gtaatctaga tccacgtccg ttaatacagt tggttgctct ttatttttta
    16141 ctgtctgagt agtaaaaagg ttgccaactt cgttttcaat agaaagtatg tcttctccac
    16201 tttcaccaac aacttgttct attgtatgtt tctctatcgt agcaattccc aatgcatatt
    16261 tttctttttc aaacattggc aaagaaatca ccatttcgat tcctttgttt ttcttttgcc
    16321 aaaataaatt atttttgtta tttatgtact tctgttgata cttttcaact tcctcttcgg
    16381 tcagttgact caacgatcca ttgattactc cccatttacc agccaaactg actaaatcat
    16441 aattattatc atccataaca atcgtttcta taaaatttag ttctttcctg atttcagaat
    16501 aagtggagta atctgtatat gtcagcggat gcttgaatat gtccgagaaa tttttggtcg
    16561 aaccatacgt agaaaacgaa tattcaatcg ttctaatttt ccattcaacg ttagttttaa
    16621 cttgcctgag caaattcttt ttgtctgtta aataactcga ttcgatcgat tcataggtat
    16681 tctggtaaat gaataaacta aatcctatga caagtacggc tcctaataaa aacatcggta
    16741 aaaatatttt ataaaatact gtacttttct tcattttc
//
LOCUS       DACDMM010000054        16701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDMM010000054 DACDMM010000000
VERSION     DACDMM010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.054"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            510..1154
                     /locus_tag="IWD16_001346"
                     /pseudo
     CDS             510..1154
                     /locus_tag="IWD16_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
     gene            1382..2671
                     /locus_tag="IWD16_001347"
     CDS             1382..2671
                     /locus_tag="IWD16_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9088414.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            2747..3628
                     /locus_tag="IWD16_001348"
     CDS             2747..3628
                     /locus_tag="IWD16_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9088415.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            3618..4433
                     /locus_tag="IWD16_001349"
     CDS             3618..4433
                     /locus_tag="IWD16_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9088416.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            4449..5573
                     /locus_tag="IWD16_001350"
     CDS             4449..5573
                     /locus_tag="IWD16_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9088417.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            5551..6354
                     /locus_tag="IWD16_001351"
     CDS             5551..6354
                     /locus_tag="IWD16_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9088418.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            6805..7077
                     /locus_tag="IWD16_001352"
     CDS             6805..7077
                     /locus_tag="IWD16_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP9088419.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            7061..7396
                     /locus_tag="IWD16_001353"
     CDS             7061..7396
                     /locus_tag="IWD16_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9088420.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(7575..8789)
                     /locus_tag="IWD16_001354"
                     /pseudo
     CDS             complement(7575..8789)
                     /locus_tag="IWD16_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            8990..10264
                     /locus_tag="IWD16_001355"
     CDS             8990..10264
                     /locus_tag="IWD16_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9088421.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(10647..11375)
                     /locus_tag="IWD16_001356"
     CDS             complement(10647..11375)
                     /locus_tag="IWD16_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9088422.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(11353..12927)
                     /locus_tag="IWD16_001357"
     CDS             complement(11353..12927)
                     /locus_tag="IWD16_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9088423.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            13156..14094
                     /locus_tag="IWD16_001358"
     CDS             13156..14094
                     /locus_tag="IWD16_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9088424.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            14106..15017
                     /locus_tag="IWD16_001359"
     CDS             14106..15017
                     /locus_tag="IWD16_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9088425.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            15041..16513
                     /locus_tag="IWD16_001360"
     CDS             15041..16513
                     /locus_tag="IWD16_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9088426.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
BASE COUNT         5311 a         2667 c         3392 g         5331 t
ORIGIN      
        1 ctgtctgtga ggacagcttt ttttgtgggc taggcatagc aaaaaggggt agtgacagtc
       61 cactgggagg gtgcacatcg accaatcgct agagaaacgc aaggtggtat ttgtgagaag
      121 atgacggaag aaatcctgta tcaaagactt ggttcaacga gcagaaatct gatagtaagt
      181 ctcggtcatt caatgggaat aagccattga acaccttgat ggtatcctgt aagaaagaac
      241 ctcaaggact ttcagcaaga agaaggaata gctgaaaaca aaaaaaggtg tagtaggtta
      301 aaagcgggga atgtagaact acttaattgg tgaaactagc tagaagggca aaatcgacga
      361 ttcatgagtt cgtaggaagg agcatgcaag atttacaact agtttactga aaaaacgcag
      421 ggaatttaca agaaattaac aagaaattaa cgcttcatcg gtatagtgga cgtgtgggag
      481 aaaatcacgg aattaaagga ggggtcaaaa tgaccatgat cactgcacac tctggtagtg
      541 acaatacgga ggagaatagc tgggcatttc ttcgcttttg tttggatcgc cagctttgtt
      601 ttgaagtaga tgtgcgtcag caaacagatg gtacgctaat actagcccat gacaggtaag
      661 aagttgtccc agattcggta acccttgagc aggtgtttca agcagtggcc aatgagaagg
      721 caaccatgac cattaactgt gatttaaaag aagagggatt ggaaatggcc gttttttcgc
      781 tggcacaaac ctatcaaatc tggccacaaa ttgttcttag tggctatgta caattatctt
      841 gggctgcgat ttggggaaag catatatttt ataatattga aaatcagttc gaagggaaaa
      901 aacttgggga tattaccgaa gcagatttga tcgatacctt acagaaatta gcctatcaaa
      961 gcgttcaaat cgtcaatatt catgaaggat ggttaactaa gttggcccgt gaagtggggc
     1021 agcagctagg attgacattt tctgtatgga cagtcaatga actagaaacg attgagtacc
     1081 ttgcgcgctg tggtgtttat aatataacct cacgttgtgc atgggcctat ttatggaaga
     1141 aaggagcaca gaataaacta ttttataatc aaaacagagg agttaagaga tttggagatg
     1201 ggatacatga ccatatgggg gctttattct tagcttgatg gatatgtcca gtgaccctgt
     1261 accccgaaat gcacaaattt cgaattattt acagaaaatt aactagaaat ttacattggt
     1321 agttcataat atttcttgat tagaaaaatg gccataaaga aattaggagg aagttaatgt
     1381 tatgttaaag aaaaatcttg ttataggggt tgtgtctgtt ttaagtgtca tctctttggc
     1441 agcttgtggt agtgggaata ccaagacaac aaaaaaatcg gagggtcgtg taacgataga
     1501 gtattggcat gttaatgcgg ataccctagg tggaaaaaca gtgaatgaat tagtagataa
     1561 atacaatcaa tctcaagaca aagtgaaggt tgtagcaaaa tataatcctg atatgtataa
     1621 aggcttgatg caaaatttac aagcttctgt tgcctccaaa gaaataccgg acgtagtaca
     1681 agtgggatgg gcttttaaag attattttgc agaaaatttc aaatatacta atcctcaatc
     1741 tttagtagaa gaagtggatc cggataatgc tgattatttt aaagatcatt ttttagacaa
     1801 tatcatggca ctgtctgaaa aagatggtaa acaagtgggt attccttact ccattagtaa
     1861 tccggtttta tatttaaatc aagatatatt aaaagcagcg ggcttggatg aaaatggtcc
     1921 aaaaacttgg gaagaagttc aaactttttc taagataata aaagaaaaaa ctggaaagta
     1981 tggcgtgtat attcaagaac ctgctgataa ttgggcacaa caagcaattt tagaaagtaa
     2041 tggggcaagt gtaataaaag atggcaaagc tacctttgct tctaaagatg gtatcgctgc
     2101 ttatgaatta tatcaaagga tggtaaaaga ccagacggca ttacacacta cgtgggaaca
     2161 aggtgtgcaa agctttattg atggtaacgt cgcgatggtc tataccacta ttgctcaaag
     2221 ggacaacatt caaaaaaata gtacttttac tgctaaagca gttgagtcac caagttggga
     2281 agggaaaaaa gttaaactac ctgctggtgg cgcaatgtta gctgttacat cacaaacgga
     2341 taagaaaaag gaagcaactt gggatttctt aaaatatctt tatagtgttg aatctttagc
     2401 taaatggaca gaaggtactg gttatttacc accacgtaaa gatgttgcag acgcccaaaa
     2461 tggattaaaa gctttcttag ctgaaaatca gatgatgata ccagcaattg atcagatgga
     2521 taagatggtc agttggagtt catttcctgg agatgctgga ttagaagctg aacaaaaatt
     2581 gttggatatg cgggataaaa ttttatcagg taattccgtc aatgaaacga tgaaagctac
     2641 tcaaaaggaa attaatgagt tactcaaata agcaaagcaa gcagacgaca gcgcctgtaa
     2701 gaaaggaaaa acaaggtcgt ctgcttttga cataaggatg aggaggctgt cggaaatgaa
     2761 gcgctatgta ggttatttgt atctggcgcc agcaattatt ttgttgggcg tgtttgtttt
     2821 ttatccattg atttatacga tctatttgag tttttttgac tggaatatgg tggcccccac
     2881 taaagaattt gttggctttg ccaactatgt aaaggtgctg actgatccag tctttcataa
     2941 agtgttgaag aatactttgt cctataccgt tctatttgtc ttgctgagct gtgtgttccc
     3001 atatattaca tcctttttat taggaatagt tatagaaaaa gggcagaatt tgtataaacc
     3061 attatttttt attccagctg tgatttcttt ggtagtggcg gggatggttt ttacatggat
     3121 attaaatccg gtatcgggac caattgctgt cttgttggga aattttggtt taagactgcc
     3181 tatgtggatg aatttacaag gttgggtaat tgctgtgatt gcaggtatta ctgcttggaa
     3241 agtttttggt tataatttca ttgtcatttt tgcagcaatt gtgggaattg atcaagagat
     3301 gatcgaagca gctaagttgg atcgtgtacc catctggcgc atttttttta caattgtgtt
     3361 gcctatgagt agttcgacag gactatattt attggtgatg accattgttc aaggtctgca
     3421 gtatgtgttt actccgatta agatcatcac tcgtggtgga ccgaataatg gtagtgcaaa
     3481 cgtgatctat tatgcttatc aaaaggcttt tgaaatgtat aaagtcggag aatcttcagc
     3541 aatttcagtt ttaactttat taatatttgc tgttttctta ggggtaatat ttaaatttgt
     3601 ggaaaagggg gtttactatg aaaattaaac gctggttgcc acatctctta tttatgttca
     3661 tcataatcat tattatctat ccattattgt ttattatggc caattctttt aagccattga
     3721 aagatgccta tcagacgatt actagtttga taccacagcc gtttaccttg gaaaattttt
     3781 gctatttgtt cgaaaatctt ccaattatca aaattacgag aaatactttt tttattgcta
     3841 caattataac ggttgttaag ttaggcgtag ctttttttgc ggcatatagt tttacttatt
     3901 gccgaatcaa agggaagaaa atctggtact ttatcatcat tggtacgatg ttcattcctt
     3961 ttactgttgc gatgattcct aattatttgt tgattgcaaa acttaattta attgatacta
     4021 cttggggtgt gataatacca caatttgctg atgcgatggg cattttttta ttgacgcaaa
     4081 caatgcggga aattccagtg agccttctag atgtggcggc attggataac atcaaacaaa
     4141 caaccgtcat gagtaaaatt gtttttcctc ttactagaca tgcgattact tctaccggta
     4201 tttggttttt tattacctct tggaatgagt atgtttggcc ggtattaatt ttacggacag
     4261 tagataatta tactttgccg ttggcgatgc agacctatat tagttctgaa ggtgggacaa
     4321 attttaccgt agcaatggcg attagtttaa ttacaatgtt tgtaccgtta gtcttgtatg
     4381 caatttttca aaaatatatt atcgggactt ttgtttctgc aggtattaaa taaattggag
     4441 gacaaatcat gggagaaacg cttgaattaa gaaatatcgg aaaaaaatat ggagaacata
     4501 ccattttaaa agaaattgat ttagctgtgg ccgctggaga acgaattgtg ttactgggac
     4561 catctggttc aggaaaatct actttgctga gaatgattgc tggtttggaa aaaattacga
     4621 gtggagagct ttttttaggt acacaactag caaataagct accttgtgga gaacgagaga
     4681 tcgcaatggt ttttcaaaat tatgctttat atcctcacat gactgtaaag gagaatatta
     4741 tttttgcatt aagggcgaat aaaattaaga cagctgacat tcagcaccgt ttggaagaag
     4801 ctttgaagat gttaggttta acgccatacc aagatcgact tcctaaagat ttgtcaggag
     4861 ggcaacggca gcgtgtagca ttggctcgtg cggtggttaa acggagtaat tattttttat
     4921 tagatgaacc gttatcaaat ttggatgtgc gtttacgatt ggatgcacga aaagaattgg
     4981 taaaaattca tcaacgttat aaacaaactt ttgtctacgt cactcatgat caaattgaag
     5041 cgatgacgtt agctgaccgg attgttttat tgaatgaagg tagaattcag atgattgata
     5101 cgccgaaaaa tgtttatacg aaacctaaaa atacttttac tgcaaatttt attggttcgc
     5161 caggcatgaa tattatgcca gttacttatg atcaaggaac attaaaggta gccagtcaat
     5221 ttttaacatt gaatccagct tggcaaaaac aacttcttca ggttagtggc tcattgtatt
     5281 taggcataag accagaacac gtaacctttt caaaagaaac aagcgattta cacggtcaag
     5341 tgaagtatcg tgagctatta ggtcaaagtt atgcgtacac cgttaaagtc gatggtatag
     5401 aaatgattgt ccttaatgaa aaacctactt ggcagattgg tgatgcagtc aatctccgca
     5461 tagaacctga aagaatgcat ttttttgatt atcagacgca agaaaattta ggatatcctg
     5521 attattttat tgaagaaaga gggaatagcc atgcgctttt tgcatctctc tgatatccat
     5581 tttagagaaa actatcaaac taacttgttt actgctgaac tatttccaac aattaaaaat
     5641 ccacagttgg cattggaaaa attgttacaa gaactctcgt taacagtgta cgattttatt
     5701 ctattaacag gtgatttagt acatgaaggg gggatttctg attatcaagt cttacggaaa
     5761 atgttggatc aatatttccc aaccacacct tactattttt gtcgtgggaa tcatgatcgt
     5821 cgcagtgttt tctttgaagg aatgagtgtt gaaagcaatg aaacgggaga gtatatccat
     5881 tgtacgaatc aaaatgggtt acgaattatt tccttggatt ctgctcaaga tacccagcac
     5941 gaaggaaaaa tatcgatagg acagatgatt caattacaag agtggttggg tacacctgct
     6001 gaaaagggaa cgattttatt ggttcatcat ccattgtcgt gggaagatcc atctattgta
     6061 actgaagttc cggaaggttt tgctgaattg attgccgaaa gtgatatttt aggtattttt
     6121 gtaggacata tccatcaagg tagcagtgct gaatttgctg gtaaaaaaca gtttatggct
     6181 gaatctttat catttggtgt agatgaatac ccagactatt ctctttttac caatagaaca
     6241 ggattcacta gtggcagttt aattgacgga aaaattttta cttatcagca ttatttaact
     6301 ccacatcaaa tagaaatagg gcgcatgaaa aaaccaatta ctggtaatca ttaaaaatta
     6361 ttgattaaaa ataaagactt cattcacttt ttagccgtgg tgctcaatgt ctggttccac
     6421 ttgaagaact tggaaaatcg ctatcaataa accactatcc ctccaattac aaaaagggtg
     6481 gagaaagttt tccaagaacc atatcggtct tcgtggttgt gccattcgtt ttttaccaga
     6541 gcataccaca tctttgtgga gttttcagac acgtcctagt taatttggtt cttttaggag
     6601 tcttttcctg ttctgtgtgt cgttgagtgt tcgcttcaat tatcgctgtg gcggattttg
     6661 aaacttttct atctatcaag gccatttagc actatatcct ttccttatat ttagataaat
     6721 acactgcatt tcaaggagtt caaaagtagt tgtattaatt ccatttgaac tctttccgtg
     6781 atataatgtg tttgtcagaa agaggtggag aaaatggcta agttacaaat ttctatggac
     6841 gaaaaattga aaaatgaagc agaaagcatt attgatgaac taggcatgag ccccaaaaca
     6901 gccgtcaccg ttttttataa gcaaattgtt gagcaaggaa aaatcccatt ttctattgaa
     6961 ttgtcggaaa aaagtaaacg aatccataat attcaaagat tagcggaaaa attgcctgtc
     7021 caaaaactag acactgacga aaaaattgaa gaatggttca atgaagatga atactaatga
     7081 tatagcaacc gtctacgttg ctttcacagc tgcagagggc ggtaaacgcc ggcctgtcta
     7141 tattctaaaa aatggactgg acacaatttc gtttctgtca attacttcaa aatatcatac
     7201 taaatccgat aaaataaagc tgaaatacgt tgaaataagg gattggaaac aggcaggact
     7261 aaaaaaagaa tcttggattg acgtaggaac tctaaacgaa ttacccaaaa acaacaaaaa
     7321 ggtcaaatgg aaaaaaattg gggaattaag tccaagcgat ttacagcgac tggaagaacg
     7381 catggaagaa ttataataat actaaaaaga agggctcttt gtcaataagg actgatgggt
     7441 tgtacaaaat caaatctggg tcagaatgaa cctcattctg gctcagattt ttggctcttt
     7501 gtcaataagg actgatgagt tgtgcaaaat caaatccggg tcagaatgaa ccacattctg
     7561 gttcagattt tttgttatct tacattgatt aattgattga tcaaaaagat tctaattctc
     7621 atgttctcga atgatttaaa tccgtaggat actcgtttca ttgtctttat gtgggtattc
     7681 ttcgcttcaa tttttccatt ggaataagga tagatcattg cgctggtgat accttcttca
     7741 taggtcagaa ggttttgaag cttctcccga aagccgtcat ctaacgtttc gggaagttct
     7801 gccaataagg agaaaaataa gtcagggtct ttgtctcgaa aggcttcgac taattcatga
     7861 aaaaagggat acgcctcctt tagaggcgca gaaaactcaa gcaatcgatc aatcatcatt
     7921 gcttcagtga gaaatgggta ttttggtgct cggaaacttt tccatgtttt gtactcataa
     7981 tggttgatgc ttgcacgatt ttttagcaga aagcgccagt tctttttcag tttttctgcc
     8041 tgacttttct gtcctgcttt acgaagctca ttcatttcac ggatacgcaa ctcattgagt
     8101 gcctgattca tgtgtttgac aatatgaaac cgatcaatca ctactttcgc atttggcaga
     8161 acacgtttgg taagctggaa gtaagcggcg ttcatgtctg tgactaagaa ttctacttct
     8221 tctggattgg tgcagcctaa gaaatagctt gttaatcgag gtaatttacg cgtaggcaag
     8281 acagcaatta agtttcctgt ttcaccatct gcgcaaataa agctcatttt atcttctatg
     8341 gaagcgtgcg aacgaaattc atcaaccatc aatactctgg ggagaatctt cttagaaggc
     8401 tttggtaaat agcttttaaa ctctttcaat gtacgagtaa cggtagtcaa agatacctga
     8461 caacttttcg caataaaaga caaagatact ttttcagtca gtaaagaagc aattttatat
     8521 cgaacatgat ctgcgattga atggctgagt cggacaaaat aactttgagc cgtccaatgg
     8581 gtacgacagt ttttacaggt atagcgttgc ttttttaggc gcgtaaccaa aggcatatga
     8641 ttgtattgtt caaaacggac aatcgtttcc ttcttcccat ttttcactat aattgctttc
     8701 ccgtttccat ctaccacagt agaaccacaa cttctacaag cacgaggagt aggcgagaga
     8761 acagcatcga cgaccaacgt ctttttcttg gctctttgtc aaataggact gatagagtga
     8821 tataagaaca tctaaggtag aattgatttt gcttctataa tattttgtgt tggtggaaaa
     8881 tcgaaggcga ttttctacta acactttttc ttcgtttcaa ctcaaaaaga gactcacttt
     8941 tggtaaaatt tagtcgacca aaacgtcatc ctaccaaagg agtctctcaa tggataatca
     9001 tactagaaaa ttactcggat taacagataa acatttattt tttgatgaag aatggctgat
     9061 tgaaaaagaa tataaaggcg ttcaagcgca gtttattaaa ggaaaattgg atgactctcc
     9121 ttcctattgt gaacactgtc gatcggttcg tattatccgc aacggttcct atacgacacg
     9181 cactcagata ttgaaagtca aagaaaagtt aactatttta gaattaaaac gtacccgttt
     9241 tctctgccat gattgtggaa ggaccttttc tgcgaaaacg gacttagttg atgaacatca
     9301 tcaactaaca aaggaactga agcaggcaat tctcatggaa ctgtacgaaa atcaatcacg
     9361 taaactaatc gctaagaagt acttcgtctc ggatgggacg gtgacacgga tcttgcgtga
     9421 agcaacgaag cattaccagt cacgtatgaa cttccttccg acggtgttgt gtatggatga
     9481 gttcaaatca atgaagtctg tttctggttc catgagtttt atttgtgttg atgggacaac
     9541 ccatcagcta tttacgatcc ttgaagatcg cagactttac aaactaactc aatacttcct
     9601 gcactttcct agaaaagccc gtttaaaggt caagtaccta gtgatggaca tgaatgccag
     9661 ctattgtcag ttgcttaaaa cagtctttcc aaatgcggag atcgtaactg accgctttca
     9721 tattgtccaa cacatcaatc gcagcttcaa tcaattacgt gtccagatca tgaatcgttt
     9781 tcgaacctca cactctgaaa atcagaagaa gtaccgtcgg ctcaagcgat attggaaact
     9841 cttactcaaa gattccacga catgggagcc actcaaacgg cattatcatc gactgtttaa
     9901 acgaccaatt agtcaaacag aaatcgttga tgagctgtta agctacaacg aagaattgcg
     9961 aactgcgtat catttttgtc aattattacg ctattatttc gttaaacgag gtacggaagg
    10021 ctttcaagaa acccttaaaa caatttcatc tacgttacct cagtcgttta aaaagaaatt
    10081 cacgatcttc cattgctacc aatcgggtat ttcaaatgcc tttaaggtgt cccactcaaa
    10141 cggcgtcact gaggggctga ataacaagat caagctgatt aaacgaattg cgtttggtta
    10201 tcgcaacttt tataactttc gggcacggat ctatttacaa caaggtctga tctttgaaaa
    10261 ctaaaaaaat atgcggcgca cagaacgcgc cgcatatcgg actaagtttt attcaaattt
    10321 gtcttcacca acacgatttg acgaagagcc tatttaaata actacatcca tttattctat
    10381 cttctaaaat agcctttttc atttcagtta aatagttcag cgccattttt gcactcgact
    10441 tgcatagtag ttttttccat attcctcaac ctcagcgggg gttgaagaca taaggaaaat
    10501 cctatgcgtt atgctcaact ggatctgatt aaatattcta atgtattttt ctctaccatc
    10561 attaaatggt taatagccaa aaatcaccac ttttcaatgg ctccttgttg tataagtata
    10621 actagaagat gaacacgtct gagaaattat agaagctttt tacggtaatt actggggctg
    10681 tgtccagtag tttttttgaa gagaacagaa aaatagctca cgtgatctat tcctactttt
    10741 tgactaattt catcaataga taagaatgtg tctttcagta gagcacttgc tcttttgatt
    10801 cgaaagtcta gcaagtaatc atgaaaggaa taacctgttt cttttttgaa ggcccgtcct
    10861 aaataagcgg gatggtaatg gaattgtgta gctacatctt tgagcgtaag cttttcacta
    10921 taattgtttt gaacgtaatc aatcacttgt tgaattgact cattaaaaga atcatcagtt
    10981 agagagtttt gattagctgc tgatttacgc attttttctt cttgtactaa cttaactaaa
    11041 taaggaaaat caatgggttt cgttatgtaa cttttaatag ataaatcaat cccctttttt
    11101 gcatactgaa aattttggtg ggcactaatg acaatactag taaagtagcg attggatgtt
    11161 tgttgcaaag attcgattaa ttcgagtcca gaaattttcc ccatcgtaat atctgtgata
    11221 agtaaatcaa agcgctggtt ttgcaggttt ttaaatgctt cgtctccgtc tgtgtagcag
    11281 tgatcgataa tgatgtcggg gactttcttc aaaagataat gccttatgcc ttctacaatt
    11341 gtacaatcat cgtcaacaat tgcaattctc aacatgcata ttcccccctt tttgtatttt
    11401 gatagtaatc gttgttccga cgtgtagttg gctttctata acaaaacgtg tatctccagg
    11461 atagagatgc tttatacgtc gaaaagaatt tgagatgccg atattctcac tataaggaat
    11521 atcatcatta agtttttgtt gaatatgctc cagcttgtct tggtccattc ctttcccatt
    11581 ttcaacgatt tttaacacat aatggttggt tgtttcagtt gcatagacag tcaaacgatt
    11641 ttcctttttt gatgaatcga agccgtgttt aatataattt tcaacaaatg gctgtaaaga
    11701 aagttttggt aacattaatt gatcaatatg atcaatcagc tgatagtcaa agttatgtcg
    11761 ataacgacgt tgatgtagtt gcaaggagag gtcgatatta attaattcct ctttcaaatc
    11821 aattatgtcg ggctgatatg tattattgcg atacaacttg ctcaataaat atatactgtc
    11881 gctgatttct tcgtcatcat tcaatatggc agtcattcga ataaattcca aattattaaa
    11941 tagaaaatga ggctcgattt gatattgaag tgctgcatac tttgcttctt gcttcaacca
    12001 attattttca taaagtgttt tttgatatgt agcattatct aaataaaact gattgatatg
    12061 catagcgatt tctttcaaat caccgacttt gttttcgata ttgattgact tgtagtctcc
    12121 tgtagatgaa tgatttaagg aagtaatgat ttcctgatac tgctggatat agttacgaaa
    12181 acttctattt aataattgat gaatagcaag aataaacaaa ctcattaata agaaaatcat
    12241 gattgccaga cctaataagg tttgtgacga ataatttgga ttttcatata cttgtaatgt
    12301 gtggttaccg atagctatct gttgcgtttt tttccattta gaaggcgaga tgtcttcaga
    12361 taaattgacg gaatcaatac cattagacaa agaataattg taatgtgttg gaaagagact
    12421 cttttttagc gcgttgatcg aaacgtctag aatcaatgta gcatcgggca tgtcctcact
    12481 aatgaaactt aatgaacgaa taaaagaaat tttttcgttt tttgtaggtg cgtaggcact
    12541 taagtatttt tcgtaatcta atgagataga tatattttta acttcatcat cttgcacaag
    12601 tttttttaag ttatctacta aataggtagg ggagcgatcc tttgacaaaa tatcttccat
    12661 gtattcagca ggtgatgaga ggtagtagtc attaaggttt tcttggaaat cataattttg
    12721 atagatctct gtcacgatac cggagagaat acgattatat gtttccatat agtgggtgat
    12781 ttgctgaatg ttttgcaggt tttgattttt ttctttttca atcacattga tataggaaaa
    12841 gaacaatgtg aaacaggcaa gtaaagtaat aaaaaaaatc aatatgtatg taaaacgcat
    12901 aataactttt gataaatcgt ttttcattca aagtcctcat tttgataatc tctaatttta
    12961 tgaatcatta tagcacatat tacaagtgat gtaaacgctt tgcaaacagg gctacttatg
    13021 attgtgaata cagtttccgt ttaaaaacta ttctattgta gaggtttaat aaactaaaaa
    13081 taatgtataa aaaattaatg taagcgatta accaagtggg tataatcaag gaaacgctga
    13141 gagaggagag acaaaatgaa gctaaagaaa gggttattag gaaatctaaa aaaaaaccga
    13201 gcattgttta ttatgctttt accaggtgcg ttatggttat ttatattttg ctatatacca
    13261 atgtttggag ttgtaatcgc ttttaaagat ttcagaattt catcaagggg attctttaca
    13321 agtttgttca acagtccttg ggttggctgg gaaaatttca aatatctctt tgcttcgcaa
    13381 gatgcttgga tcattactcg caatacggtt gtttataacg ttgtaggtat tttgctaagt
    13441 actttttgta cagtgtcatt agcaatcatt ctgagccaaa tggcgaacaa acggttattg
    13501 aaaaagatgc agacatttat gatttttcct aattttttat catgggtagt catcagttac
    13561 tttgtttacg catttttgag tacggataaa ggattaatta atattttact aagcaatttt
    13621 ggtgtggaac ccataaattg gtatagtgaa ccgaagtact ggccttttat tattgttttt
    13681 acgagtttat ggaaaggcgt aggctatgga tccatcattt attacgcgtc aattatgggg
    13741 atcgatacta gttattatga agctgcttta gttgatggtg ccagtaaatg gcaacagata
    13801 aggaatgtag tcttgccaca tttaagatcg attattgtca ttatgttgat tttgtctgtt
    13861 ggtagtattt ttcgagctga ttttggattg ttttatcaat tgccaagaaa ttcgggggct
    13921 ctttatgatg tgacgaatgt attagatact tatgtctacc gtggattaat gaatagcgga
    13981 gatatcggaa tgtctacggc agcgggatta tatcagtcat cagttggctt tgtcttgatt
    14041 ttgattacta ataagatagt caacaagtta gatcctagtt cagcactatt ttaggaggaa
    14101 tcaaaatgat atcaaaatta aaacggcaga tgtacttttc gctgccagtc gaacggtttt
    14161 tcacaatatt actaatctta tttactctta gctgtatttt tccctttatt ttcgtaatcg
    14221 tcatttcatt aacagatgaa aaagcattac aactcaacgg ttacgaatta ttgccggctc
    14281 aatggagcac tgatgcatat caatacttaa tacaagatgg cggacagtta cttcggtcat
    14341 taggcgttac aattatgatt acagtgattg gaacgttgat tactgttttt atgacgggaa
    14401 cctatgctta tgttttatca agagcctctt tcccttaccg gaagttcttt actttttatc
    14461 ttttttttac catgttattt gcgggaggga tggttccttc ttacttagtg atgacgaaga
    14521 tgcttggctt aaaaaataca atttgggctt tgattctacc tcttgctttt agcccataca
    14581 atgtcattat tttaaggaca ttctttaaga agtcgattcc agaatcgatt atcgaatccg
    14641 ctaaaatgga tggctgtagt gagtttcgag tttttttcca aatcgttttg cctttagcga
    14701 tcccaggggt tgcaacaata ggtttatttt caagcttagg ttattggaat gactggttca
    14761 acgcattgct gtatattgac accaacaaat tgattccatt gcaatatttg ttaatgaaga
    14821 ttcaaaattc aatggaattc ttagcaaata ataatgacat tacattagcg cagcaacaag
    14881 caattcaaaa tagccttcct caagaatcaa cacgaatggc aatggtcgta gtagctactt
    14941 taccgattgc aattgtttat ccgttcttcc aaaaatactt tgttcaagga ttgacgatcg
    15001 gtggagtgaa agaataaaaa agaataggag atgttaaaag atgaaaaaga acatgcagaa
    15061 atatttgatt ggtggtgctt ctgtactaac ccttggccta ttaggtgctt gtggtagcag
    15121 taatggcgaa gcaaataatg aaacaggaac cacaaaagtt acttggtatc aggttggaga
    15181 agaacccaaa gattttgatg aggtcatgac aaaagtcaac gaaaaattaa aggaagaaat
    15241 caatgtagag ttggatatgc ggctgattcc ggacggggat tatcagcaaa agcttggtgt
    15301 gatgatcaat tcaggagaag aatatgatat ttgtttcgtc aatggcacag attatgtgaa
    15361 ttatgggaac aaaggtgctt tcattagtct caacgatcat ttggataaag agttgaaaga
    15421 ttatgcagct gaattgaatg aaggatttat tgaaggtggg gcaattaatg gcgaaactta
    15481 tggattgcca gtcaataacg atattgccac aacggaatat tggaccttta ataaagattt
    15541 ggtagatcaa taccaattgg atgtagagaa tgttgaaggt gtagaaacat tggctccagt
    15601 attagaaaag tttgcagaaa aaaatactga tacgaatatt acgccaatcg gtatgacccg
    15661 taccagtgtc tgtattcctt atgacaatat tatcggtggt ggtgtaccgc tagcggttgc
    15721 atttgaaggc gatacacaaa cagttgttaa tacttatgag actccagaaa tgatgactta
    15781 tttgaaggct atgcatgagt tttataaaaa aggatatatt ttgaaagatg cagccactgt
    15841 tacgacagat ccatttcgaa ttgatagtaa aaactggttt ttaggtcgta actcaagtgg
    15901 ttatcaacca ttagcacaaa aaaaattaga agctctagcg ggacatggat taattttaaa
    15961 accagctgga aaagctttta aaacgactgg atttgcaaga gtggcaatgc atgcggtatc
    16021 tgcttcaagt aaacatccgg aagcagcctt gaaattgttg aattatgtga ataccaatca
    16081 agaagtagcg gatttaatgg tttatggaat tgaaggaaaa cactatgatc gcaatgaaga
    16141 tggttcgatt actttaacaa actctgatga ttattatcaa ggcgcttggg cattggtaaa
    16201 tacttatcta gtttcaaaaa cttcaccgat tacagaagaa agtaaaaaac tagaagaaga
    16261 aagtaaaaag tttcaaaatg aagcaccgat gtctcctatt ttaggtttct cttttgacgc
    16321 ttctgatttt cgaacagaaa ttgcaagtgt acaaaatatc gtgtcacaat atgaagtact
    16381 tttaagctcg ggaacagttg accctgaaca atatgtacca gagttgttag aaaaactaga
    16441 aaaagctgga ttggataaaa ttcaggcatc tgctcaagaa caatacgatg aatggcgaaa
    16501 atctaacaaa taatttttta ctaaatgaaa agaatagact aagcagttag tggggggatt
    16561 caaatgaacg aaaacaaatg gaccaaagga tttaattggg tttttcaatt gttgttacta
    16621 aactttactt attttatact aatttgttta ggatttggaa ttttaggttt tttccctgct
    16681 acgatagctt tgattgctgg c
//
LOCUS       DACDMM010000055        16204 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDMM010000055 DACDMM010000000
VERSION     DACDMM010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.055"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            86..943
                     /locus_tag="IWD16_001361"
     CDS             86..943
                     /locus_tag="IWD16_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP9088427.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1058..1978
                     /gene="pstC"
                     /locus_tag="IWD16_001362"
     CDS             1058..1978
                     /gene="pstC"
                     /locus_tag="IWD16_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP9088428.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1978..2862
                     /gene="pstA"
                     /locus_tag="IWD16_001363"
     CDS             1978..2862
                     /gene="pstA"
                     /locus_tag="IWD16_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP9088429.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2877..3683
                     /gene="pstB"
                     /locus_tag="IWD16_001364"
     CDS             2877..3683
                     /gene="pstB"
                     /locus_tag="IWD16_001364"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9088430.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3699..4457
                     /locus_tag="IWD16_001365"
     CDS             3699..4457
                     /locus_tag="IWD16_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9088431.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4470..5147
                     /gene="phoU"
                     /locus_tag="IWD16_001366"
     CDS             4470..5147
                     /gene="phoU"
                     /locus_tag="IWD16_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP9088432.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5385..6416
                     /locus_tag="IWD16_001367"
     CDS             5385..6416
                     /locus_tag="IWD16_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9088433.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6413..7180
                     /locus_tag="IWD16_001368"
     CDS             6413..7180
                     /locus_tag="IWD16_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9088434.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7280..8848
                     /gene="liaX"
                     /locus_tag="IWD16_001369"
     CDS             7280..8848
                     /gene="liaX"
                     /locus_tag="IWD16_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP9088435.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9148..9465
                     /locus_tag="IWD16_001370"
     CDS             9148..9465
                     /locus_tag="IWD16_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP9088436.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            9465..9815
                     /locus_tag="IWD16_001371"
     CDS             9465..9815
                     /locus_tag="IWD16_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9088437.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9982..10920
                     /locus_tag="IWD16_001372"
     CDS             9982..10920
                     /locus_tag="IWD16_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP9088438.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10935..11765
                     /locus_tag="IWD16_001373"
     CDS             10935..11765
                     /locus_tag="IWD16_001373"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9088439.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11809..12834
                     /locus_tag="IWD16_001374"
     CDS             11809..12834
                     /locus_tag="IWD16_001374"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9088440.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12849..13787
                     /gene="galU"
                     /locus_tag="IWD16_001375"
     CDS             12849..13787
                     /gene="galU"
                     /locus_tag="IWD16_001375"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9088441.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13912..14352
                     /locus_tag="IWD16_001376"
     CDS             13912..14352
                     /locus_tag="IWD16_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP9088442.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14355..14957
                     /locus_tag="IWD16_001377"
     CDS             14355..14957
                     /locus_tag="IWD16_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9088443.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15016..16122)
                     /locus_tag="IWD16_001378"
     CDS             complement(15016..16122)
                     /locus_tag="IWD16_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9088444.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
BASE COUNT         5250 a         2824 c         3501 g         4629 t
ORIGIN      
        1 tttgttattt aatgattttg attcgttttc atcaagtaat gaaaatgata aaataaacat
       61 actttaggat taaaggagat ttctaatgaa aaaacttaaa ttattcttac caataatagg
      121 attagctatt cttttatcag gctgttcaaa gtggattgat cgaggagaat ccattacggc
      181 tgttggctct tctgctttac agcctttagt agaaacagtt gctgaaactt accagacaga
      241 aaatcctgga aaattcatca atgtacaagg gggcggaagc ggaactggtt tgtctcaagt
      301 acagtcagga gcagtcgaca tcggaaactc tgatttgttt gcggaagaaa aatcggggat
      361 cgatgcctca gcattagtcg atcatcgagt agcagttgtt gggatcactc ctattgtgaa
      421 caaggatatt ggcgtaaaag acatcagtat ggaaaacctg aaaaaactat ttacaggcga
      481 gatcaaaaac tggagtgaag tcggtggaaa agatcagcca gtagttatat tgaaccgcgc
      541 ttcaggaagc ggaacccgta gcacgtttga aaaatgggta ttggacggag aaacagctgt
      601 tcaggcacaa gaacaagatt caagcggaat ggtgcgccag atcgttgccg acacacccgg
      661 tgcaatcagc tatgttgctt tttcttatgt ttccgatgat gtagatacgt tgaatgttaa
      721 cggtgtagaa ccgacagatg aaaatgtgac aacgaacaaa tggacgattt gggcatatga
      781 gcatatgtac acaagagatc atccaaaaaa actgacaaaa gaatttttag actatatctt
      841 atctgatgaa gtacaagata acatcgttgg agaactaggt tatatccctg tttctcatat
      901 gaaagtcgaa cgtgattggc aaggaaatat cgttaaagag taatctttac acaaaattta
      961 caaacttagc taaactttta catgttatta aaaccaaatt gatgttcaat ttgtacactg
     1021 agtgagtatg ttaaacctga ttaaggggga gtcatctgtg gaagatgtga aaaaagtatt
     1081 attaacaaaa tcaaaacgtt caaaaatgga acagcgtgga aaattcatca gttttttatg
     1141 tatcgcgctg atcgtgttgg ttgtattatc tattttttat tttgtagcta gtaaaggatt
     1201 ggccaccttt tttactgata agatcaatgt atttgatttt ctgtttggta cagattggaa
     1261 tccaagccaa atcggagcaa atgggaaacc aatggtaggg gcattgccta tgatcacagg
     1321 ttcgtttatt gtgacctttt tatctgctat cgttgccacg ccatttgcca ttggtgcagc
     1381 agtattcatg acggagatct ctcctaaaaa cggaacgaag atcttacagc cggtcatcga
     1441 gctgcttgta ggtattcctt ctgtcgttta tggttttatc ggtttatcag ttatcgtgcc
     1501 ttttgtcaga agtatttttg gcgggactgg gtttggtatc ttagctggta catttgtgtt
     1561 gttcgttatg atcttgccga ctgtcacaac gatgactgtt gatgcattaa aggcagtgcc
     1621 acggcactat cgggaagctt cattagcgct tggtgccaca agatggcaaa caatctacaa
     1681 agtcgtgcta cgagcagccg tgcccgggat tttaacagca gtcgtttttg gtatggctcg
     1741 tgcgtttggt gaagccttag ctatccaaat ggtcatcggt aatgcagcat tgatgccaac
     1801 aggtcttact actcctgctt ctacattgac gtccatctta acaatgggaa tcggaaatac
     1861 aatcatggga acagttgaaa ataatgtgtt atggtcatta gcgctgatct tgctattgat
     1921 gtcattattc tttaatatcg tgatccgtat gattgggaag aaaggagagc tgaaataatg
     1981 aacgcaaaac gctcagataa aattgctaca ggtgttttat atatagtttc ggggatcatc
     2041 gtattgattt tagcagcttt actactttat atcttagttc ggggaatacc gcacatttca
     2101 tggtccttct tgaccgaacc ttccaaagcc taccaagaag gcggtggaat cgggatccag
     2161 ctatttaact cgatttattt gcttttgatc acgatgatca tcagttttcc tatttcttta
     2221 ggtgcgggta tctatctttc agaatatgct ggaaaaaact ggctaacaga tgtgattcgc
     2281 acgtcgattg aaatcttgag ctcgttgcct tctgtcgttg tcggtttatt cggtttcttg
     2341 gttttcgtta ttcaattcca gtatggtttc tcgattattt ctggtgcatt ggctttgact
     2401 gtattcaact tgcctctttt gactagaaca gttgaggagt ctttacaagc ggtccattac
     2461 acacaacgag aagcaggatt ggccttaggt ttgtcacgct gggaaacagt gataaaagtg
     2521 gtggttccag aagcgacacc agggattttg actggtgtga tcctaagttc tggaagaatt
     2581 tttggtgaag cagctgcatt gatctatacg gctggacaaa gtgcacctgc gttagatttt
     2641 acgaactgga atccattaag tgtttcaagc ccgatcagca ttttccgtca agctgaaaca
     2701 ttagctgtcc atatttggaa gatcaataca gaaggcacga tgcctgatgg tgtttctgtt
     2761 tcggctggcg cttctgccgt gttgattatt gctgttttac tgttcaactt cggcgctcgt
     2821 gcgattggca aacgattata ccgaaaaatg acttcagcct aaggagaaga agcaaaatga
     2881 aaaaatataa ctggaatgaa acaaatgtca tcaccatgcc aaaagagaaa gaagtagcac
     2941 tatatacgga tgatctacat gtctggtatg gcgacaatga agcaatcaaa ggagtggatc
     3001 tggaatttga aaaaaataag atcactgcac tgattggtcc ttccggctgc ggaaaatcta
     3061 cgtatcttcg ttcgttgaat cgaatgaatg atgggatcgc taatacgaaa atcactggga
     3121 aaatcatgta tcaaggcgta gatattaatg cgcctcaagt ggatgtttat gagatgcgca
     3181 aacggattgg catggttttt caacgtccta atccgttcag caagtcgatt tatgacaata
     3241 ttacatttgc tttgaaacag cacggcgaaa aaaacaaaaa gaaattagat gaaatcgttg
     3301 aaacaagctt gaaacaagct gccttgtggg accaagtaaa agacagcttg gacaaaagtg
     3361 ccttggcttt atctggcggg caacagcaac gtttatgtat tgctcgtgcg atcgcgatga
     3421 aacctgatat cttgctgcta gacgagccgg caagtgcttt ggaccctatt tcgacaggaa
     3481 cggttgaaga aacacttgta aacttgaaag aagactatac gatcatcatc gtgacgcata
     3541 acatgcaaca agcagctcgt atcagcgact atactgcctt tttctattta ggaaaagtaa
     3601 tggaatatga caaaactaac aagatattta ccagaccaaa aattcaggca accgaagatt
     3661 atgtgtccgg tcattttggt taagacggag gcctcaacat gacaaaagag attatttcat
     3721 ctaaagatct tcatctgtat tatggaaaga aagaagcact aaaagggatc gatctaagct
     3781 tcaatcaagg ggaaatcact gcgatgatcg gtccttctgg ttgcggaaaa tccacttatc
     3841 tgcgttcttt gaaccggatg aatgacctga ttccgaatgt aacgatcacc ggaagtgttt
     3901 tatataaaga caaagacatc tatggtccaa aaacagatgt tgttgaacta agaaaagaaa
     3961 tcggcatggt attccagcag ccaaacccat ttcctttttc tatctatgaa aatgtcatct
     4021 atggattgaa attaaaaggg gaaaaagata aaaaagtcct tgatcaagta gttgaagaaa
     4081 gcctgaaggc agcgtctgtt tgggatgatg tgaaagacaa attgcataaa agtgctttat
     4141 ctttatccgg tgggcagcag cagcgtgtat gtatcgcacg tgttttggca gttaatccgg
     4201 aaatcatctt attggatgaa ccaacaagtg cgcttgatcc tgtttctact gggaaaatcg
     4261 aaagtatgct gttagaatta agagagcggt atacgatgat catcgtgacc cataatatgt
     4321 ctcaagcttc acgtatttca gataagacgg cattcttttt agatggtcac ttgatcgaat
     4381 acaatgatac gaagaagata ttcatgaatc cagaaaaaca agagacagaa gattatattt
     4441 caggtcgatt tggataagga ggaaacggaa tgctacgtac acaatttgaa gaagaattgc
     4501 tgaatctcca taatcagttc tacgaaatgg ggatgatggt gagcagcgcc gtgcacaagt
     4561 cagttcgtgc atacatcaaa cacgataaaa tcttagccca agaagttatc gaaaacgata
     4621 taaatatcaa taacatggaa acacgcttgg agaagaaaag ttttgaaatg atcgcattgc
     4681 aacagccagt tacaactgat ttgcgtatga tcattacggt catgaaagct agctcggatt
     4741 tagaacgtat ggccgatcat gcggtgtctg ttgctaaatc cacgattcgc gtaaaagggc
     4801 agacgagaat accggaaatc gaaaaagaga tctccgatat gtccgattat gtgaaaaaaa
     4861 tggtggacaa cgtcctagtt gcttatgtca aaacagacga aaaagacgca cgtacgatcg
     4921 ccaatatgga tcaaagagtc aatgactatt tcaatcgaat ctataatgca acaatcaaaa
     4981 atatgcagga aaatcctgag acagtcatca gtggaacaga ctacttgaat gttgccagtt
     5041 atctagaacg gatcggagac tatgtgacaa acatctgtga atggatcgtc tacttggcta
     5101 cagggaaaat cactgagtta aatacgaacc acaatgagat tttctaaact gaagctgtga
     5161 ttatgccgtt tgtttaggaa aagaggttgt gaaatcagcg gttgtgaaaa agctgttctg
     5221 catcaagtaa taagaacagt caaacaaaaa atagctcctc atatttttcg tttggctgga
     5281 cttattaccg cagatgcaag cttttgaaca ccgtttattc gggaaagggt ttgtgacaaa
     5341 acatgtcaca aacccttttc atcatttcat agaaaggata aagaatgcaa aaaaaaatta
     5401 gcatcatcac tatatttttt atttgtgtgc ttacttcatg ggttattcag catcaagccg
     5461 ctttatatga agtacctgtc atcgagatag aaaaagtacg aataaaagag gatcatcaat
     5521 tgattacagg aaaactgatg aacacggatc aaaaaggaga atcgatttat ttatcggttc
     5581 cttatcaaaa aaatcaaata gaaacagcaa atataaaagc gagacaaatg tatctagtcc
     5641 aaatgcatgg agatgaatgg cagctagtag caaaaaaaaa tgatggctgg cttttcttct
     5701 tcacaagtat cttcattggc gccatgttgc ttattggtgg aaaacagggt ctatttgctt
     5761 taggaagtct catcatcaat atcgttttac ttttactttt gttgctcatc tttactaaaa
     5821 cagaagggat ttctctatta aatatatcta tcctgttcgt cgttataggg atgattatta
     5881 gcatattggc tttagatgga tggaatagaa gcagtataat caaaatttca gctgcagtta
     5941 cgagtgtttt tttagcattt tttatttgtc ttttgacaat gaatcattgg aaagaccaag
     6001 gactgcgcta tgaggaactg aattatctga cacgaccgta tcgttctgtt tttctttcta
     6061 gcgtactgat tggaacagct ggaggaacac tcgataccgt tatcacagtc attgcgacat
     6121 tagaagaatt gactaagcga caaccagaaa taacagcgaa acagttatgg ctttcaggaa
     6181 ggcaagtggg tcgagatgtg attggctcga tgaccaatat ccttttgttc gtctatatca
     6241 gtgggtcagt tccatcgctt cttttatatt taggaaatca atggtcgttc aaagaaacga
     6301 tagaacagca tttatcattg gaatttttac gcgtcattgc tggcagctta ggaatagtat
     6361 tatcgatccc tatctcggtc ctcttttttc tgactgtaag gaggctgaaa cgatgaatgt
     6421 acttattata cttgttggga ttttagcaat cagcgtgcta cttattggcg gtacgcaagg
     6481 tatgtatatt cttttaggct tattcatcaa tttagggatc tttttcctgt tgttattcgg
     6541 ttatcatcag aagtggccga tactggcatt gtcggtcata ggatttttat tgattgctgt
     6601 ggttatctta ttttttatca acggttataa tctaaaaatg cgtgcagctt ttgtgagtat
     6661 actgatcttt ttattttgtt tcctactatt gcttcctatc actgattttt tagcaatcca
     6721 aggttttaca tcggtagaac tagaagaatt atctggctta gataagactt tagcgattga
     6781 ttttagatta cttacgagat cgttgcttct gattagtctt agtggtgctg ttttagatgc
     6841 gagtgtagca atttctagtg ggacattcga agtatatcag gcaaatccgc aattatcttt
     6901 ttaccaattg cgccattcta gtttcgctat tgctaaaaaa atattggcgt caacagtaat
     6961 gacacttctt tttgcattca tgggaagttc tttagcgttg atcctctggt ttattgattt
     7021 agacattcct tttcagcaag tgatcaatga aaaatcattt gttctagaat atacgatggc
     7081 tattcttacc acattgtccg cactacttgt tcttccttta acctgcgtga ctacagcata
     7141 tctctttaca aaaaagaaag aacaactcaa gatggagtaa aagttacaac ttaagtccta
     7201 tgacattttt actgaatcag tgcataatat ggatgtaagg aaaagcttgc aaacaatttt
     7261 gcttaaggag gcaatttcca tgaaagaaag agaacgtatt ttagatttag tgaaaaaagg
     7321 aatcctttcg actgaagaag gcttagattt attagaaagc atggcaactg aaaaagacga
     7381 aaagcaaatc aaaaaagaag cagacaaggt aactgctagt cataaagaaa aagatcaggc
     7441 aagtcaattg atcgataaac ttgaaaacgg cgaagaagaa atttctgagc cagttgatcc
     7501 aaaggaaaga gaaagacaag atcaagaaaa cttagaaaag atcttagatg aactagcaac
     7561 tgaagcgaat aaaacttcgg cgcgtctaga tgaagtaaat gctcaattcg ctgacaataa
     7621 ggcagcacgc aatgagaaac aggaagcatt gatgcagctg aatacaaaag aagaattagg
     7681 cgagttgaca gaagaagaat tagcacagcg tcagacgtta gaagcagaaa tcaaagaatt
     7741 agaagctagt ggcgatacac tgttagaaga acaattgaaa ttagaagcag aattaaaaga
     7801 tatccgtaag aatcaatggt ctgagaaaaa agaaacgttt actgataagt ttgaattgcc
     7861 agatgattgg aaagatcaag caacagatac actgaatcaa gtaggcgaaa aaatgtcaga
     7921 agctggatca cagttaggca aattcttgaa gaaaacattc caaactgttt cagaaacagt
     7981 gaatgacaac atggaatgga aagatgtcag cttacgtgtc cctgggattg cgacaacgaa
     8041 atttgagcat gaattttact atgaagctcc tgcagcaagt atccttgata tcaaggcagc
     8101 aaacggaaac gtgacgttaa aaacgtggga cagcaacgat gtgaaagttg aagctaagat
     8161 caaactttac ggaaaaatgg gtgctgaacc atttgaagca ttctctgaac ggagccagat
     8221 tgaggttaat gaagatcata tctccttcca aattccaaat aaacgtgtgc gtgcagacct
     8281 tgttttctac ttacctaaac gagtatacga ccacgcagct attaaacttc taaatggcaa
     8341 catcatgatt gaaacattgg aagctaaaga tatttataca aaatctacga atggtaacat
     8401 catcgtggac caattaactg caacgatgct ggaagtagaa ggtgtaaacg gcaatatcga
     8461 tattcgaaac gggaacatct tagattctat tatcgaaaca gtaaatggta cagtgacatt
     8521 tggtgcgaca gcagaaaacc tttctgtgtc tttggtaaat ggtgatgtac gtcttacgat
     8581 taaagaagat aatttgaaaa aagtagaagc aagttcagtg aacggaaatg tcaaagtcgc
     8641 tttaccagat ggcatcggtt tggaaggaca tgctaaaaca agtcttggaa gtatcaacag
     8701 ccgtctgtcc aattacgaag tagttcgtga gaaaaaagaa cggacaaacc aaatgttgca
     8761 attccgtcgt gtttcagatg gtgaaatcgc gcaagtgcaa ctttcaacga caacaggaag
     8821 tatctattta aaagatacag ataaataaag aggttgtgaa agagatgttc agccttgagc
     8881 aataagaagc gattttgtaa aatagcttcc cctattttac aaaattgtgc cttattgccg
     8941 atagtctatc tcttgaacac cgtttaccaa gagattatga acctgccaat tatcgccgag
     9001 tcacaaggaa taatttcaga aaaagctcgt cacgttttat gaatatggta atttgtgacc
     9061 gagaattttg cagaaagaca ccgattaaga agaaggttgt cacaacgatt ttcttcattt
     9121 aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc taataaaaat
     9181 gttgttttga cagggacttt agcaggaata ggagaataca tcgggattga tccgactgtt
     9241 atccgtgtgc tttatgtatt cctgagctta gttgcttttg gaagtccaat tattttatac
     9301 attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta tggacataac
     9361 aatgcatatt atgaaaaaaa caattatcgt gaacaaactc gtgaacaaac aaaaaaacgc
     9421 aaagaagcgg aaaaaatcga tgatgacgaa tggagtgact tttaatgtcg tactttcaac
     9481 gtattttagt taatacgtta acattcatat cattggctgt tctttttcct gcgatggtcc
     9541 atgtgagcag tatttggacg gccgttcttg ccagcttcgt attgtctgtg ttgaatatgt
     9601 ttgtcaaacc gattttgacg atactgtccc tgccctttac attagtcaca ttgggattgt
     9661 ttacgtttgt tatcaatgca gcgattctta aaatgacctc cttttttgtg ggagcagaac
     9721 attttagttt ttcttcattc ggtgcagcgt tgttagtagc tatcttgatg tcgcttgtca
     9781 atgtaattgt cagcgaacat aacagtagaa aataaaagaa agctgtgccg aactatcggc
     9841 gcagtttttt tatttagaat caggaatgcg ttttctatta cattggtaat taaataaaaa
     9901 cgtttttttt aattggtagt acttttggct agacaatgat aaaatgatag agaacactct
     9961 aatagaaatg aggtagcatg aatgtcagaa gttgtaaaag ttagccaatt agtgaaaaaa
    10021 ttatctttgg aaatcgtgac aggcgacgag aaaagcttag accgtgtcat cgtaactggg
    10081 gacatctcac gcccaggact agagttgact ggttatttta attattattc ccataatcgt
    10141 ttacagctat ttggcagtaa agaaatcagt tttgcggaac ggatgatccc agaagagcgt
    10201 ttgatcatca tgagaagatt atgtagtgaa gatatgcccg catttattat ttcaagagga
    10261 ttaccagctc cagaagaaat gatccaggca gcaaatgaaa atgggatagc tgtactgcgt
    10321 tctcccatct caacttctcg tttattagga gaattatcca gttatttaga tggtcgcctt
    10381 gcccctcgaa caagtgtcca cggtgtttta gtggatgttt acggattagg tgtactgatt
    10441 caaggtgaca gtgggatagg aaaaagtgaa acagcgttgg agttgatcaa acgcggccat
    10501 cgattgatcg cagacgatcg agtagatgtt tatcagcaag atgaattaac ggtgatcgga
    10561 gaaccgccta aaatcctgga acatctgatt gaaatccgag gaatcgggat cattgatgta
    10621 atgaatcttt ttggtgcgag tgcggtacgt ggctctatgc aagtccagtt ggctgtttat
    10681 ctggaagcat gggcaaaaga taagaaatac gaccgtctgg gctctgaaga cacgaccgtt
    10741 gaaatcgcgg aagtgggcat tccacaaata aaaatccctg tcaaaacagg acgaaacgtt
    10801 gcaatcatca ttgaagtagc cgcgatgaat ttccgcgcac gtacaatggg ctttgacgca
    10861 acgaaaacat ttgaagaaag attaagtcgc ttgattgaag aaaactcagg caacgactag
    10921 caaggagtga caatatgtta gcacaaatca atcgagttgc ttttgagctt gtagggattc
    10981 ctatctactg gtatgcattg atcatcgtat caggtatcat tatcgctatg tggcttagca
    11041 gcagagaggc tgttcgggta ggattaaaag cagatgatgt gacagatttt atgttgtggg
    11101 gattgcctat ctccattatt ggcgctcgct tatattatgt tttatttgat cttccccagt
    11161 atatagctga tccaatccaa atattcaata tccgttcagg aggcttagct atttatggcg
    11221 gactgattgc aggcgcgatc acgctgtatt tttttactaa atatcatttt atttctttgt
    11281 ggactttttt agatatcgct gcaccaagtg tattattagc acaggcaatc ggtcgttggg
    11341 gaaatttcat gaaccacgaa gcatatggac cagacacgac gcgaagcttc ttagaaagtc
    11401 tgcatttacc gcaattcatc attgataata tgtatatcga agggatctat cgtcaaccaa
    11461 catttcttta tgaatcactt tggagtttga ttggatttat tttgcttttg tttttacgaa
    11521 agaaaccaca ccttttgaaa aaaggcgaag tccttttatg ttatctaatg tggtatagtt
    11581 tcgggcgttt ctttattgaa ggattacgta cagacagtct ttacttttta ggtgtgatcc
    11641 gtatttctca attattatct gctgtgttat ttgtcggtac attgattcta tttatttggc
    11701 gcagaaaaaa aggaaattta ccggattatg accgctcatt aggaaaaaat caacaaatta
    11761 tctgattttt agagcgttaa aaatgaaaaa aatttcagga gtggtaaaat gaattataga
    11821 gtagcggttt taggaccagg ctcttggggt actgctttgg cacaagtcct cgccgaaaac
    11881 ggacatgacg ttcggatttg gggacatcgt gcttcacaag tagaagaaat caacacccaa
    11941 cacacgaatc aacgttattt accagatcat tatctacccg attccatcca agcattcacc
    12001 aatatggaag atgcagtaaa agacgcagat gcggtgctat tcgtcattcc aacgaaagca
    12061 atacgttctg ttgcaaaaga actagtgcct aaattgaata caaagccagt gatcatccat
    12121 gcaagtaaag gattagaaca gggaacacat aaaaggatct ctgacatctt gttagaggaa
    12181 atcccagcag aaaaaagaaa agctgttgtt gttctttctg gaccaagcca tgcagaagaa
    12241 gtagcaaaac atgacatcac tacgatcaca gctgcaagta cagatgcaaa agcagcaaaa
    12301 tatgtacaag agctgttcat gaataattat tttcggatct ataccaatcc tgatgtgatc
    12361 ggtgtagaga tgggggcagc tttgaaaaat attattgcta ttggtgctgg cgctattcac
    12421 ggcttagggt ttggcgataa tgcaaaggca gcaatcatga cgcgcggatt agctgagatc
    12481 agccgtttag gtgtagcaat gggtgccaac ccgctgacct ttatcggatt aagcggaata
    12541 ggcgatctag tcgttacttg cacaagtgtt cattctagga actggcgcgc aggcaacttg
    12601 ttaggacaag ggcagcccct agaagaggtg ctagccaata tgggaatggt cgttgaaggt
    12661 gtgaatacta cccaagcagc gatggaacta gcccaaatga tggaaatcga aatgcccatc
    12721 actcaaacaa tctatgaagt gctgtatgaa caaaaagaaa tcaaaacagc cgcaaaagaa
    12781 atcatgttac gtgatggaaa aaaagaaaat gaattctcat taacaatttt ttgaaggagc
    12841 gaatgtatat gaaagttaaa aaagcagtta ttccagcagc aggattagga acaagatttt
    12901 taccagcaac aaaagctatg gcaaaggaaa tgttgccaat tgttgataaa ccaacaatcc
    12961 agttcatcgt tgaagaagcg ttgaaatcag gtatcgaaga catcttgatc gttacaggaa
    13021 aagctaaacg accaatcgaa gatcactttg attcaaatat tgaattagaa acaaacctaa
    13081 gtgaaaaagg aaagaccgaa ttattgaaat tagtagaaga aacgacagat gtcaacttgc
    13141 atttcatccg tcaatctcat ccaaagggtt taggacatgc cgttcttcaa gcaagagcct
    13201 ttattggaaa tgaaccattt gtcgttatgc taggcgatga tataatggaa gacgaagtac
    13261 cattgacaca gcaattaatc gatgattacg aacgtactca tgcttctacg atcgccgtta
    13321 tgcaagttcc tcatgatgaa acttcaaaat acggaattat caatccagga gaagtattgg
    13381 aagatgggtt atacaatgtc aaaaactttg ttgaaaaacc agatccaagc aaagcaccaa
    13441 gtgatttagc tattattgga cgttacttat tgactccaga aatttttgat gtattgtccg
    13501 agcaacaacc aggagccgga aatgaaatcc aactaactga cgcaatcgat acattgaata
    13561 aaacacaacg cgtatttgcc cgtaaattca caggtaaacg ttacgatgtc ggtgacaaat
    13621 ttggttttat gaaaacaagt attgaatatg gtctaacaca tccagaagta ggtgcacctt
    13681 tacgtgagta catcctgtct ttaggtaaaa cattagaagc agaaggaagc aagatcgatc
    13741 ataatccaaa aggaataaca gaaatcaaag aagaaaagaa aaaataaccc ttttttctaa
    13801 cgagcttcag ctgctaattg aataaaaagt agttgaagct cgtttttatt tgtttaaaaa
    13861 acacgaaaat gctatcctta tagaaaagag agacaagaag ggagtttgat catgtcagga
    13921 ggagaaattg caggattgat cgcagcaatc gcctttgcga tacttgtcgt ttttatcgtt
    13981 cgtgtgttgt tgcaagtttc taaaacttta gaaaaagtag atcagactgt agcagaagca
    14041 aatacgacga tcgaaatcgt cacaaaagac gtagatcttt tgtctagaca agtagaaggc
    14101 ttactggtca aaagtaatga actgctcttg gatatcaatg gaaaagtcgc aacgatcgat
    14161 ccattgttca cagcagttgc tgatttaagt gaaagtgtat cagaacttaa tcattctagt
    14221 aaaaacatcg cgacgaaagt cggcggaatc ggtaaaacga cagctaaagc gacggttgcc
    14281 ggaaaagtcg gtgggacagc gatgaaattt tttaaaaaca aaaaacatac tgaaacgaca
    14341 ggaggaaaat aaaaatggct aaaaaaggtg gatttttatt aggtgcagta gtcggaggca
    14401 cagccgcagc aatcgccgcg ttattgttag caccaaaatc tggtaaagaa cttcgggatg
    14461 aattagctgc ccaagcggat gatttgatgg atatggcttc agattacaca gatatggcga
    14521 aagaaaaatc gactgagtta acaggtattg caaaagaaaa agcgcaagat ctttctcaac
    14581 aagcaggtga tctagcaggt agtgtcaaac aaaaagtaag tgaatcagca gatgaagcag
    14641 gaagtgaatc tgacgatatc ttgttaaatc ttaaagaaca atcagctgac ttatctaatc
    14701 gtttcaaaaa aacggcagaa gatatgaaaa aacctgcaga tgaattagga agtattgcta
    14761 aagatacttc agaagatatc atgattgatg taaaagacgc tgctggtaaa gcaggagata
    14821 ctgtcaatga aggaatgtca gaagcaaaac cagttgcaga tgactcaaaa gaaatgatga
    14881 aaatggcaaa agaagatgcg aaagaagcta aaaaagaaat aaaagataca atgacagatt
    14941 catctacaaa tcactaaaat cacaaattta tagaataaaa aggtgggagc ggactagctc
    15001 tcaccttttt tactcttatt tttctaaaac aagtaattct tttggtgtag tcgtgaatgg
    15061 cacacagcca tcgcttgtca catatacaca gtcttcgata cgtaccccta ctttttcagg
    15121 aatatagatt cctggttcaa gagagaagca catcccttct tcgattacta agtcatttcc
    15181 ttctacaagg gaagggaatt cgtggacggt tgttccgatc ccgtgaccta aacggtgatt
    15241 gaaatattcg ccgtagccat agctgttgat cacgtttcgt gcgatttgat caagttcacc
    15301 tgctgtcaca cctggacgga cggcttcttg tgcggcaagc tgtgcttcaa gagtgatgtt
    15361 gtagattttt tcttggaagt cggttggttt ttgataagca attgtacgag tagcatcact
    15421 gcaatagcca ttccaaacaa cgccaagatc gaataacaca agttcattcg gtgaaatctt
    15481 cgtatttcct ggtgtaccat gagggcttgc agcatttgct cctgcaagga ctaatgtgtc
    15541 gaaagacatt tgtgaaacac cttgtttttt caattggtat tcaatttctg caatgatcgc
    15601 ttgttcagtc acgccttctt tcactgcttt aaagccaatc tcaaatgcaa catcggccca
    15661 tttaccagct tcgatcagtt tgtctttttc ctctagagtt ttgatcagtt gtagacgttg
    15721 gatgatggaa gttagatcag tgtaagaatc cgttcccgtc aagatatcag agatagcatc
    15781 gaagcgctcg acagtcaagg cgttcttttc aatccctaca ttgccaggct gaccatagcg
    15841 agcacgaagt tcttttccga tgatctccca agggttttcg ctgtctaaat agccacggac
    15901 atcatacgcc caactactgt tttgagcatc ttcaacttcc aaagcaggag tgaaaaggaa
    15961 aggatcttgt tcaagaggga taaaaagagc tagtacacgc tcatgaggat cactttcgta
    16021 tccggaaaag taagcgatgt ggccagggtc gctgatataa gccaattcga cttggtgttg
    16081 tgccatccat tgacgtagtt cattgatttt tacgttattc atttggcaac ctactttcta
    16141 aattaatttt gtattcaata attgtaccac tgatttatta aaagaccaac gttctaaaaa
    16201 ctga
//
LOCUS       DACDMM010000056        16087 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDMM010000056 DACDMM010000000
VERSION     DACDMM010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.056"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            226..894
                     /locus_tag="IWD16_001379"
     CDS             226..894
                     /locus_tag="IWD16_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP9088445.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IWD16_001380"
     CDS             complement(1094..1480)
                     /locus_tag="IWD16_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088446.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IWD16_001381"
     CDS             complement(1452..2834)
                     /locus_tag="IWD16_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9088447.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IWD16_001382"
     CDS             complement(2990..4177)
                     /locus_tag="IWD16_001382"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP9088448.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IWD16_001383"
     CDS             complement(4184..5215)
                     /locus_tag="IWD16_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9088449.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IWD16_001384"
     CDS             complement(5231..6685)
                     /locus_tag="IWD16_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9088450.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="IWD16_001385"
     CDS             6860..7765
                     /locus_tag="IWD16_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9088451.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="IWD16_001386"
     CDS             7856..8533
                     /locus_tag="IWD16_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9088452.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IWD16_001387"
     CDS             8814..9710
                     /locus_tag="IWD16_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9088453.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IWD16_001388"
     CDS             complement(9765..11135)
                     /locus_tag="IWD16_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9088454.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IWD16_001389"
     CDS             11373..12119
                     /locus_tag="IWD16_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9088455.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IWD16_001390"
     CDS             12116..12955
                     /locus_tag="IWD16_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9088456.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IWD16_001391"
     CDS             12956..13906
                     /locus_tag="IWD16_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9088457.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="IWD16_001392"
     CDS             14047..14799
                     /locus_tag="IWD16_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088458.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="IWD16_001393"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="IWD16_001393"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP9088459.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5219 a         3009 c         3232 g         4627 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgct
//
LOCUS       DACDMM010000057        15951 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDMM010000057 DACDMM010000000
VERSION     DACDMM010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.057"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(387..809)
                     /locus_tag="IWD16_001394"
     CDS             complement(387..809)
                     /locus_tag="IWD16_001394"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP9088460.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(823..2229)
                     /gene="atpD"
                     /locus_tag="IWD16_001395"
     CDS             complement(823..2229)
                     /gene="atpD"
                     /locus_tag="IWD16_001395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP9088461.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2252..3154)
                     /locus_tag="IWD16_001396"
     CDS             complement(2252..3154)
                     /locus_tag="IWD16_001396"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP9088462.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3168..4724)
                     /locus_tag="IWD16_001397"
     CDS             complement(3168..4724)
                     /locus_tag="IWD16_001397"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP9088463.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4747..5289)
                     /locus_tag="IWD16_001398"
     CDS             complement(4747..5289)
                     /locus_tag="IWD16_001398"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP9088464.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="IWD16_001399"
     CDS             complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="IWD16_001399"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP9088465.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="IWD16_001400"
     CDS             complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="IWD16_001400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP9088466.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="IWD16_001401"
     CDS             complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="IWD16_001401"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP9088467.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7180..10023)
                     /gene="rnr"
                     /locus_tag="IWD16_001402"
                     /pseudo
     CDS             complement(7180..10023)
                     /gene="rnr"
                     /locus_tag="IWD16_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10026..10772)
                     /locus_tag="IWD16_001403"
     CDS             complement(10026..10772)
                     /locus_tag="IWD16_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9088468.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10884..11120)
                     /gene="secG"
                     /locus_tag="IWD16_001404"
     CDS             complement(10884..11120)
                     /gene="secG"
                     /locus_tag="IWD16_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP9088469.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11238..11516)
                     /locus_tag="IWD16_001405"
     CDS             complement(11238..11516)
                     /locus_tag="IWD16_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9088470.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11629..12597)
                     /locus_tag="IWD16_001406"
     CDS             complement(11629..12597)
                     /locus_tag="IWD16_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9088471.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12812..12856
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12880..13854
                     /locus_tag="IWD16_001407"
     CDS             12880..13854
                     /locus_tag="IWD16_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP9088472.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13859..14434
                     /locus_tag="IWD16_001408"
     CDS             13859..14434
                     /locus_tag="IWD16_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9088473.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14501..15640)
                     /locus_tag="IWD16_001409"
     CDS             complement(14501..15640)
                     /locus_tag="IWD16_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP9088474.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4357 a         3443 c         2856 g         5295 t
ORIGIN      
        1 ctgagccaga atgtggttca ttctgactca gatttgattt tgcacaactc atcagtcctt
       61 attgacaaag agcccaaaag tcttgtcgca gtcccttatt ccaaataaac ggtgttcaat
      121 catctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttccatttg
      181 tttctctaat actcaggaca aaacgctgat ttcacaacct ccgatatacg gtgttccaga
      241 agtagcttct tcgaaaaata agctgagatt tcacaaaaat ttgagaagca attttcgtaa
      301 attccttctt atgtcttgaa gctgaccact tctgtcataa cctcttttta ctgttcttaa
      361 acacggataa acatccgcat ctcattttat gagtgcttag atactttgat acgattgatt
      421 gctcggtgca acgcaacagt tgcccgtttc aattcgttcg tatcttgttt tgcttttgct
      481 tcttcgattt ggcgttctgc gcgttgtttt gcacgttccg cacgagatac atcgatatca
      541 cgttcacgtt ccgcactatc agcaacgatt gaaacaacat tatcacgaac ttccatgatt
      601 ccgccattaa cagcgatcca atcgacgtgg gtatctgaat ctgttcgttt tacacgaact
      661 tcatcaatcg tcaatggaac gatgattggt gcatgtttag gcaaaatccc aagctcacca
      721 gctgtcgtac gtgcaacgac gatagctgca tggtgatcat agaccaaacc ggcaggagtt
      781 accacattta ctgttaaata ttgatccatg gggcacccct ttctagtagc ctagtgtttt
      841 cgctttttct acgacgtctt cgattcggcc aacacttcgg aaagcttctt caggaagatc
      901 atcgtatttg ccttccaaga tttcgcggaa tcctttcact gtttcttcta cagggacata
      961 tgaacctgga agaccagtaa attgttcagc cacgttaaag ttttgtgaca agaagaattg
     1021 gactctgcgg gcacgagaaa ctaacacttt ttcttgatcg gataattcat ccatacctaa
     1081 gatagcaatg atatcttgta attcacgata tcgttgcaat acgtgttgga cttctgttgc
     1141 tactttataa tgttcttctc ccacgatttc aggtgctaaa gcacttgaag aagaagccaa
     1201 tggatctacc gctggataaa tcccttgttc tgttaattta cgttccaagt tggttgtcgc
     1261 atccaaatgg gcgaatgctg ttgccggcgc cgggtcagtg tagtcatcgg ctggcacata
     1321 gattgcttgg atcgatgtga tcgaaccttt tttcgtagat gtgatacgtt cttgcaattg
     1381 acccatttct gttgccaatg ttggttgata accaaccgca gaaggcatcc gtcctaacaa
     1441 ggctgatact tcagatccag cttgagtgaa acggaaaatg ttgtcgataa acaacagtac
     1501 atcttgacct tctacatcac ggaaatattc cgcaatcgtc aaaccagtca aagcaacacg
     1561 catacgtgca cctggtggtt cgttcatttg tccgaacacc atggctgttt tttcgataac
     1621 tcctgaatct ttcatttcat aatacaagtc attaccttca cgagtacgtt cacctacacc
     1681 ggtaaataca gaaataccac catgttcttg ggcgatatta tggatcagtt cttggatcag
     1741 tacggtttta ccaacaccgg caccaccgaa tagtccgact ttcccacctt ttaaatatgg
     1801 ggcaagcaaa tcgataactt tgatccctgt ttccaaaatt tctgtacttg tacttaattc
     1861 gtcaaaggct ggtgcttttt tatgaatttc gcttctttcc gcatcttctg ggaaaggtgt
     1921 ttctaaatcg atcgtatctc ctagtacgtt gaatacgcga cctaatgttt ctttacctac
     1981 aggaacagag attgctttgc ctgtatcaat gacttccatt cctctttgta aaccatctgt
     2041 tgattccatc gcaatggtcc ggatcactcc gtcccctaat tctaacgctg cttcaagaac
     2101 aacttttgat ttattttcgt catttttata aacaactaat gcgttgttga tatctggtaa
     2161 ggattggtct aaagaaaatt ccacgtcgac aacgggaccg attacttgaa caatcttgcc
     2221 tgaactcatc ttttttcctc caatctcaat actattctag tgccgaagct ccggcaacaa
     2281 tttcggtaat ttcttgggta atagcccctt gtcttgcacg gttgtaagaa accgtcagct
     2341 catcaatgat attagcagca ttatcagttg ctgttttcat tgctgtcata ccagcagcat
     2401 gttctgctgt tttggcgtca acgatcgcac cgtagatcaa actttcagca tactgaggca
     2461 ataactgaga caagatttct tcttttgaag gttcaaaaat atattcttgt tcgaaagtag
     2521 ttgcttcttc tggatctaag tctgagattg gcagcatttt ttctacacga aactgactag
     2581 ttagcgaatt gatatgatgg ttataacata cgtataattc atcgaatact tcgttttggt
     2641 acatagttgt tgccatattt acgattttgc ggacttcatc aaagcttggc tgatcagata
     2701 gattgcgcag ctcatatgct agattgatac cgcgtgcttt aaagaagtct gcaccagttc
     2761 cgccaattgc gatcatcacg taatcatcgg gtgattggtg gtcttcttct aaaatagaca
     2821 tcgtttgttt caatatcgag ctgttatatc caccgactaa accgccatct gcagtgatga
     2881 cgatataacc cgttttctta acaggacggc tgagcaacat gctgttgtaa ttgatatccc
     2941 ctctaggatt ttctgaggca atatcattta actgagtagc agtcaaatgc gtaacgattt
     3001 ctcttacttt tgttgcataa acctgaaact tctgtgaaga agcttcagat tttgtcaact
     3061 tagaagcaga caccatctgc attgcccgcg tgatttggct cgtttttttc gttgaagcga
     3121 tacgtgtctt gatttcattt aatgaagcac ccattcatct cacctcacta tgcattttga
     3181 atggatttca atttatcttc tgtagaggaa ttatttcctt ttgtagctaa gaacatgtct
     3241 ttatattcct gaatcgcact gttcaacgct tcttcttctg gaagatcttt tgttgtacgg
     3301 attgtttcaa agatatctga atgattggta tccagatatt cgaataattc atgttcgaag
     3361 tctaaaatac tgtctacagg gatactatct aagaatccat gtgtcaaagc atacaaaatc
     3421 actacttgtt tttcaacagc tagcggtgca tgcaattttt gtttcaagat ttctactgta
     3481 cggcgtccac gatttagttt tgcttgtgtt gctgcatcca agtcagaacc gaattgtgta
     3541 aatgcttcta attcacggta acttgctaag tctagacgta aagtcccagc aacttttttc
     3601 attgctttga tttgcgcaga accccctaca cgagatacag atagtccggc atcaaccgct
     3661 ggacgagtac ctgcgtagaa caagtcgctt tctaagaaga tttgtccatc agtgatcgaa
     3721 ataacgtttg ttgggatata agcagagata tctccagctt gtgtttcaac aaatggtaag
     3781 gcagtcattg aaccgccacc taaatcatca cttaattttg ctgcacgttc caataagcgt
     3841 gagtgtaagt agaaaacatc ccctggataa gcttcacgac ctggtggacg gcgaagcagc
     3901 aaggaaagtt cacgataagc aacagcttgt tttgaaagat catcaaaaat gatcaagaca
     3961 tgtttcccgt tgtacatgaa ctcttcaccc attgcagtac cagcatatgg tgcgatataa
     4021 agcaacggcg ctggttgaga cgcaccggca ttcacaacga ttgtgtaatc cattgcgcca
     4081 tattttttca atgtttcaac ttgtgtacga actgtagaat ctttttgtcc gattgctaca
     4141 tagatacaga tcatatcttg acctttttgg ttgatgatcg tatcgatcgc aatggaagtt
     4201 ttccctgttt tacggtcacc gataactaat tcacgttggc cgcgtccgat tggtacaaga
     4261 gcgtcgattg cttttagacc tgtttgcatt ggttcgttaa cagatttacg ttgcataacg
     4321 cctggtgcca ttgcttctac tggacgagct ttgtcagtaa cgatttcacc tagtccgtca
     4381 attggctgac ctagcggatt aactacccgt ccaatcaagg cctctccaac tggaacttcc
     4441 atgatctttc ctgttcgttt tactttatct ccttcacgaa tggactcgaa atcgccaagg
     4501 ataatgatac ctacatcatt tgattctaag ttttgcgcca ttccgtatga gccatttgaa
     4561 aattcaagca actcaccact catcgcgttt tctaaaccat gtgcacgagc gatcccgtca
     4621 cctacataag ttacggtacc gatttcttcg actgaaagca cgttttgata attttcaatt
     4681 tgttctttaa tcaaggcact aatttcttct gctttgatgg ccattcgatt cacctacctc
     4741 tttgtactat ctatttaatt gattacgcat attttctaat tgcgtgcgaa tgcttccgtc
     4801 gattactcga tggttagctt caacaatcgc gccaccaata atagaagaat caacgatttg
     4861 ttttaattcg actgattgat agttcattgt ttttgcaaca ttcttttcta attgtattcg
     4921 ttgttcgtct gaaagtggaa ctgctgttgt gacacttccc aacagcaaac cttgatgttc
     4981 atcatatcga cgttcatatt cttcgatcat gaacaaaaga tcatccattc ggttatacgc
     5041 ataaacaact tctaaaaagt ttttaacgat tccatcatag ccactaacaa gcttgtccat
     5101 gatttctcgt ttttcatgag gttctagacg aacatcactt aacatattac ctaatccagg
     5161 tacttgttca tagatttgac gtaagcttac gagatcttga taaatttctt ctgctttatt
     5221 tgaatcgatc gccagttcaa ataaagcttt cccgtaacgt cttcctactg tatatttatc
     5281 tagtttcatt tgaagaacct agaccttcga tatattgatt gattaatgat tcgtgcattt
     5341 ctggagataa ttctttgttc aagatttttt ccgcgatttg aagagatagt tctgcaacat
     5401 cgtcttttac agagttcagc gctgaatcac gttctacagt gatatcagct tgggctttac
     5461 tttttagacg agctgcttct tcttgagcat ccttcaaaat attttggcgg cttaattctc
     5521 cactttcttt cgcatttttg atgatatcag ctgcatcaga acgagaggct aacaattgtt
     5581 gttcgcgttc ttgttccatt ttcgctgagt tgatgcgaga ttgttctgca gaatccaaat
     5641 cattggcgat cttgtcttca cgttttttca aaatatcgct gattggtccc caagcaaaat
     5701 gcttcaagag agccagtagt atcaaaaatg agccgctgac aacgatgaca tttcctaaca
     5761 tcgcattgcc aacttctgca attgccaatt gattcagcat aggcgagcct tcctttcttt
     5821 gaagtacttc tagttttcat gtaaaaaagc agtaacaaac caaattgttt ctgccttaat
     5881 actgattcaa ttaaacacca aaaactagaa tcaaggcgac aactacccct aagataggaa
     5941 cagcttcgac tagagctacc ccgataaaca ttgttgtacg gaattgacca gacatttcag
     6001 gttggcgagc cattgattcg attgttttag agataacttg accgttacca taaccggctc
     6061 cgattgctgc tcccatgatt gcgattgctg ctgcgatata attcataatt gttttcctcc
     6121 taaattagat aaatagtagt ttgtttttaa aacgaatatt attcttccac ttcaatctta
     6181 tggctcatat aaaccataga taaagtaacg aagacaaatg cttggatgct tccgataaac
     6241 aaggagaatg cgatccagac catctctaat ggaattgcta acggcaatga gaaccagcct
     6301 aagcttatta ccatttttgc aatcaaggtt agcaaaactt cccccgcata aatattccca
     6361 taaagacgta aagcaagtgt taataagttt gtaaattctt caatcagttt catcggaaac
     6421 ataaaactat atggtttcaa gaacgaattt acgaaatatc ctttgaagcc taactttttc
     6481 accccaaata agtgagtaag taagatcatt ataaatgcca aagttaaagt aacaaacggg
     6541 tctgctgtcg ggcttttcca aagtgttgtt tcgttaggta aaacgatttt tgttactaaa
     6601 ccgatgttgt ttgcaacaaa aacaaataaa aacaatgtaa atgccaacaa atggaaattg
     6661 ctcaattctt ttctaggcag attatctgtt acgattcctc gactaaaatc aatgacccat
     6721 tctatcgcat tttgtttgcc tgttggtttc aacttaagat ttcttgtaaa aaagtaagct
     6781 aagaagaaaa caatcaaaca ggtcagtaac accattatac aaactgtacc atcaaaccaa
     6841 attggtccaa tgtggaacgt tagcgagcgt tcatccaatc catttcacct cttttccccg
     6901 aaattcacat aaaatcaatt catgtgcgta tctggttttt tccaatcggt ttctaaccac
     6961 acggtatcat accaccatga aacagaaaat tcaaaacatt ttcatgaaaa ttagacactt
     7021 ttttatttca gtcaaaaacg aagcgaattt ttctttatac acggtgctcc tgatctaaga
     7081 aaaaacctat gataatcatg ttttatcaga ttaaatgcta acaaatttta tcattaagta
     7141 aaagcaaaag caccgaatat gcgaaaaagg ataatgaaat tatctagaga aattttttaa
     7201 tgttcgatca atttgtcgat cgatatcttt tcgtttcaaa tcttcccgtt tatcataaga
     7261 ttttttccct ttcgccagac caatcaatac cttggcatag ccgttacgaa tatagacttt
     7321 taaaggaaca acagtgattc cagtattttt caattcattt tcaagccgga tgatttgttt
     7381 tttgtgtaat aataactttc tcgtgcgcaa cggatcatga ttaaaaatat ttccttgttc
     7441 ataaggactg atatgaacat tatgcaagaa agcttctccg ttgcggacgc gaataaatcc
     7501 atcttttaga ttgatccggc tgtttcgtat cgacttgatc tcggttcctt gcaagaccat
     7561 ccctgcttcc atcgtgtcga tgatcgaata atcatggcga gctttcttgt tttgtgcaat
     7621 taattttccc tcgccttttg gcatagctgt ttcctccaac tcattttatt ttttcttttt
     7681 cttcttttta acacctttgt aaaatggctt cttccctttt ttcttaccga acgtttgctt
     7741 actatcgcta tatcgtttgt tcttcttgcc atttctttct gggcgttttc ctgcttgttt
     7801 tccttcgcgt tttcctgatt ttccatttcg tgtttttggc cgttccaacg ctgtaacctc
     7861 ttctgcggaa acaagttcaa aatctactgc tcgttcatct ggatcagctt tgactacttt
     7921 tactcgcact ttctgtccga ttttaaaagt cagaccagtt ctttcaccta caagagctaa
     7981 atgattttca acaaaatgga agtagtcctg tttcaattca ttgatatgga tcaatccctc
     8041 tactgtattc ggcagttcga taaacaggcc gaatttcaca acagagctga tgatcccatc
     8101 gaattcttcc cccactttgt ccatcatgaa ttctgctttc ttcatggaat ctacttcacg
     8161 ttctgtttct actgcacgac gttccatctt agaactatga tctgcaattt caggaagttc
     8221 cgtagcccac gaactttgat tgctttcact tttatcttca ctgtaagaac ggatcagacg
     8281 atggacgatt agatcgggat agcgtcgaat aggagatgta aagtgtgtgt agtaatctgc
     8341 cgctaagcca taatgcccaa aattgtcttc tgaatagcgt gcttgttgca tgcttcttag
     8401 caacatagta ttgataactg cagcttctgg tttgtcttct actccttcga tcacatgctg
     8461 taaatctttt ggtgtgatcg ttcctttcgt tcctcggacc agaatgccca aagctgaagc
     8521 aaaatcaaag aagcgctgca ttttttcttc ttttggctgt tcatggattc ggtaaataaa
     8581 cggcagattt aatttattat aatgttcagc taccgtttca ttggccgcaa gcatgaaaga
     8641 ttcgatcaag cgttcaccaa ttcctctgct tcttagctgg atatcatttg ggtgtccttc
     8701 agcatcaaca aggatttttg cttcgcgatc ctcaaaagaa atcgcgccac gtttgattcg
     8761 catcgtttca aggattcgat gcaattcacc catttcttgg aacataggta cgagctgagc
     8821 atagcgttgc atcgtttctt cgtcttgatc ttctaagatt tgattgacag ctgtgtaggt
     8881 catccgttct gttgtttgga tcacacttgg gaaaatatca tgagagacta cttgtccatt
     8941 tggatcaatc tccatttcgc aactcatggt cagccttgga acatggggat tcagtgaaca
     9001 gataccattc gataaacgtt gcgggatcat cggaatcact cgatcagtca aatatacact
     9061 tgtccctctt tcatatgctt ctctgtccaa ttgactgcct tgtgtcacat agtaagaaac
     9121 atctgcaatg tgtactccaa gaaaataatt accgttatct aattttttaa ccgtaactgc
     9181 atcatctaga tcttttgcat cttctccatc aatcgtaaca atgagctgat ttcttaaatc
     9241 tctacgtcca gttatatcct tttcgctgat ttgatctgga acttcatctg cttcagacaa
     9301 tacatcatca ggaaatttcg tagggatacc attagccacg acaattgaga ggatatccat
     9361 gccagggtca ttcttatgac caatcacttg cttgattatc ccttctaaac tagtcgcata
     9421 ccctttttca gggtaatggg tcagttcgat cagcacaata cttccgtcaa ctggacgaac
     9481 tccctctgct gctgcatata ctttaaattg gttgagcttt ctatctttcg ggataacaac
     9541 gccgtaaaga tccgtttctt gtatttcttc ttctgaataa gcaacgaatt cccctgctaa
     9601 ctgagtagtc gcccggtctc gaatctcgac tactttcccc tctgctccgc gatccatatc
     9661 tgtatttgct cgcttcatga tatcgattgc tacggtgtct ccatccattg caaaaccagt
     9721 cgcttctttt gggatataga tgtcatcttc ttcgggatca atcgttacaa atccaaatcc
     9781 tcgttcattt gctcgaaatg ttccttcgat caagattggt tttaaaggaa gtttcacttt
     9841 tcctttttta ttgaaaacta tactctgttc tctttccatt tgagcaatcg tttgtactag
     9901 taatttaaaa tcatcgcttt tttggaaccc taaaccttca gcgatttctt ccatagaaaa
     9961 actgcgtttg cgactgcttt ccatgaaaaa gagaattttc tcttttatcg tttcttttgt
    10021 catatttatt cctcattcca aggtaatttt tctaaaaatt ccaagacgtc tttttctaat
    10081 tcgcgtcttg ctgtaccaac tgtaatcaca tgaccacttt caggatacca tcgaagtgtc
    10141 actcgctgac ggctcaactt gcgtgcagtt tcaaatacgc cattagggtc gatcatttca
    10201 tctttcccag cttgagctaa aaatatcggc tgttggatat ctgacaacct actttctacg
    10261 attgctgcct gatcttgtat atccattaat tgttgttcag ctaacgagcg gtaagcaact
    10321 gctttttcat tgatttcttt ctctgattta cctgctgtct tcagcacttg acgtacgtat
    10381 ttttcaaagt tttcaggcac gtttgttttt acaggagaca aaggcgaaca gaaaaaaccg
    10441 ccgccaatca ctgattcttc ttctagtgct cgtacagcaa agatgccgcc cattgataat
    10501 cctaatacgg caatatcaga aaactgctca gactgtaaaa aatggatggc ttttttagtg
    10561 tctgcccacc agctttctgc tttttcttgc aaaatatcat atggctccat tgttccgtgt
    10621 cctttaaaaa gcggcgcata aactgtataa tctgcttttt ccaaaaatcg tgcgagcatt
    10681 cgaacatcgt ttggacttcc tgagtaagcg tgaagcagca aaaccgctcg ctttccatgt
    10741 tttgcataaa ggggttttgg taattgtctc actcgtctca cctctttcgc ctcattttac
    10801 catagtttct ttcataactt cttcttaagc gttctctttg caataaaaaa agccgcccgc
    10861 cgttttgcga gaggcttatt caactacttc gaagataaat atgctaggac aaataataag
    10921 atcatccaga tagctcccat aactgcagtt gatcgctgca tgacagcttc aaagccgcga
    10981 gctttttgtt tgccgaatag ttgatccgca ccgccggtaa atgcactcgc tgcactattt
    11041 tgcttgcttg gctgcatcat aatcgcaatg accataacaa tcgataaaac gatcacgatt
    11101 cccaaaatta aattatacat atcgcagcct ccttcccaaa agatatcgtc tctttaactt
    11161 tatcatagat aaaagtccgt gacaactcta tataaatgga ataacagctg aaagttcaaa
    11221 actttcagct gttattctta tgactgacgt aagacgtctt gatattctgg tttcttttca
    11281 aactcttttt ttgcataagg gcataaagga atgatctttt tcccgtctcg tcttgctaat
    11341 tctactccag tatacactag cttttcagct aacttttgac cacgatatgc tggatctaca
    11401 aatgtatggt caatgatcat gatgtctggg cccgcatctg accatgtcat ttctccgatc
    11461 tcttgttgtt catcattgaa taaagcaaga cgattttttt cttctttgat ttccatatgc
    11521 cgcactcctc aaataactga tattttttta ttcctacctt attttagcag tctcctgatc
    11581 tataaacaat tatttatcct cgaattactt acaaaaaaga agagaaaatt aattcatcgt
    11641 tattctttgt tcaataaatg aaaaaatcac tgtccaatag gttttcggat ctttcatcga
    11701 tgcttccaag tgttctgctt ctggaacaag cagcagtgct ttatcccctg ccgtagcatt
    11761 ttgaatcgtg tacgctgctt ctgtagggac aaaatgatct ctttcaccat gaatgaccaa
    11821 taaaggcaga tggttgctgc caagctgtct cgtagctgat gcttgcttta aggagtaacc
    11881 ggcgtatttc tgggcataat gattcgcata tctcatgacc atttttttag gaaagacagt
    11941 gattttactt aacatatatc ggaattctgc ataaacagaa acatatccgc tgtctaaaat
    12001 aaatcctttg accgctgatg gaagtttttc accacttgcc atcatgatcg tagaagctcc
    12061 cattgatccg ccgtgtaaaa taatggacgc attcggctgt tcatctaaga tgagttggat
    12121 ccaggcaatt aaatctagcc gatctagcca tcccattccg atgatttctc cttcactttc
    12181 tccgtgcgca cgtaaatcag gtacaagaac gttgaatcct ttctctgcat atcttttacc
    12241 tatatgagac atgtctcttt tccctgtgct tcggtaatca tgtacgcaaa tgacccattt
    12301 attctgctta tttggatgga tgaacatctg accagctagt ttcaacccgt cataactttt
    12361 gattgttttg ggtgttccct gctcccaaaa gctttcacac tcttcttcta atttttgtcg
    12421 ttttttttct actgacgtga ctgcagcagg gtccatcaaa cgatgaccag tgtcttcata
    12481 ccagtcatat cctctctgta ctcccgctct tactaatttc tttgcataaa aataagaaag
    12541 aaaatggtaa ctgatcccta taattatcag aataagaatg aaataaaaaa taaccattgt
    12601 acacccgccg ctttctgcca aactataatc taactattat ccaatgtacc gatccatagg
    12661 attctttatt ttcggcacac gtaaattcca tttctttttc attgtatcat ttacttaaag
    12721 ttctcctcaa tagatttact tgtaagagag catttacatt cgcaataaaa gccagtatac
    12781 taatttcgtg cattataatc aagacgctcg tcttggactg gttcggaaac ttcccagaat
    12841 aaaaaactaa gtatctctaa agaaaggaga ttggaaataa tgcgaaaagt aattattgat
    12901 tgcgatccag ggatcgacga cactttagct ttatcattag ctgtcaaatc ccctgatatc
    12961 gaagtagttg ctattaccgt agtttgtggg aatgtccctg cagatatagg tacgcagaat
    13021 gtgctgaaat gcctggaacg ctgtgatcgt ttagacattc ctgtttatca aggaatggaa
    13081 aaacctttga agcaaccttt catcagtgca caagacactc atggtatgga tggtttaggg
    13141 gaaacaaatt tcccgatgat cttgagaaaa caggcagaac ctctccatgc cgttgatttt
    13201 ttggctgatt atttcaaaga aaagacaaat acatcagtca ttgctttggg tcctttgacc
    13261 aatatcgctt ttgctttgaa agtcaaccca aatattggga aacatatgga tcgttttgtc
    13321 tcgatgggtg gaacttacaa aagccatggt aactgttcgc cagttgctga atacaattac
    13381 tggtgtgatc ctgaagcagc ttcttatgta ttcgagaatc tcaaacaaac gatagaaatg
    13441 gttgggttag atgtgacaag agaaatcgtc ttgacaccga ctatcctcga atactgctgt
    13501 caaatgaatc ctgaagaggg agagtatcta aaagcgatca ctcgttttta ttttgacttc
    13561 cactggaaac aagaaagaat tttaggatgt gtcatcaatg atccgttagc tgttgcttat
    13621 tttatcgagg gggccatctg tgaaggtttc aaaagcttca ctgctgtgga aactcaagga
    13681 ataagtcgtg gacaaacatt ggttgatcgt tatgaatttt ggcagaaacc tgctaacagt
    13741 aaaattatga caaaagtaga tactcgtctt tttttccgaa agtttttaac cgttctttta
    13801 aatgcacaag aagaaacgat tatgaaagat ttggaaaggt taaagatggg ctaacaaaat
    13861 gaataaaaac aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta ttgggatcaa
    13921 ttatattgga ggaacgattg ctttatggct tcgtttgcca atctatctcg attctatcgg
    13981 aacgattttt gctggtgcat tgcttgggcc tgtacctgga atgctgacag gattgtccag
    14041 cggtatttta agtggagtga ctacagatat tttttctctg tattattcgc caatccaaat
    14101 tctcacaggc ttgcttgctg gattgatttt gccaagatat ctcggcaaag ataaaaacag
    14161 cttgaaactg ttattactcg ctttgatttt gtcggtacct ggaacgatcg tttcttcgat
    14221 cattaccgtt cagttatttg gcgggatcac ctcctcagga tccagtatga tcgttcagct
    14281 attacatggg ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg caggtacaga
    14341 ctaccttgat cgtttgttgt ctatcgtcgt tgtttcatta gtcatcacta tgttgccaca
    14401 acgcttgata tattcaaaaa gaaaaatagg ctgaataaac agagctggat tagcaaaata
    14461 atgctaatct ggctctgttt tttcatttat taaaaaaatc ttacgctaag taggataaaa
    14521 taattccttg atatttttca atcatatcca aataattatc gatttcaaca tactcatcta
    14581 cttgatgagg caaagtaacg acaccaggcc caaagacgac aaagtcgaag gaatggttag
    14641 ctttcgtaaa ttcggctgca tccgttgttg ctgccgctcc aactaaaggt aatggctggc
    14701 tgaattgttg ctgaatgcaa tgaatcaaag gagaatctgg atcagctttg acaggtattt
    14761 tgttgtaatc gatcatcaat tccaaatgat agtctgtctc ttgattcaat tcattgacga
    14821 ttgattgcag caatgcaatg attttatcat ttgggtattc tggaatcgat cgaatgttcc
    14881 cttggagctg cgcatggctt gggatgctgt tgacttgatt cccgccactg atcaatgtaa
    14941 cattatggat tgtttttcct aggacaggat tctcgattgt ttcagcgagg tgattcattt
    15001 cagcatttgc tttcgtaatg aattcattta ggtgattgat tgcattataa ccttggtctg
    15061 gcatagagct atgcgcttct tttccgtgcg aggtgactgt atagttgatt gatcccatgt
    15121 gcgtatacat caaactgtaa ttagttggct ctccgatgat caaggcatct aagtcatcta
    15181 catagcctgc tttcgtgagc tgttccccac ctagttcacc cacttcttct ccgacagtgg
    15241 ctaataattt gactgttccg ttaaatggtt ttcctgactc tttcagttcg atcatggcaa
    15301 tcaccattgc tgccaagcca ctcttcatat ctgtagcacc acgtccgtac aaacggttcc
    15361 catggatttc tgctgcaaag ggtgcatagg tccaagacga ttcatcacca gccgccacga
    15421 catccatatg cccagatagg cctaacactt ttcctgattg gcccttttgg taacgagcaa
    15481 tcaaattatc tctaccatct ctgtaagaaa caatctctcc tgttatgtca tgctttgcca
    15541 aaagcttatt caaataggct gctacttcac cttcgtttcc attcactgat ttaattcgga
    15601 tgatctcttg taaaatcgcg attttttctt cttttttcat ctaatcctct cccttgccat
    15661 ttctttttat tgtatttgag tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat
    15721 gaagaaagag agccacttta tgaaaaatat gctgctactt ccctcctatt ttatttatac
    15781 gagaggactt gctcatagat tcctattcta ctgttctatc cttaaaagct cttatatgat
    15841 gctagttatt gttagctttc ctttttcaag gatgatcttt tcttgtacca ttctttgtaa
    15901 acatcatata cagaagctag ttgcaaaaaa aactctctac ctgctgatat t
//
LOCUS       DACDMM010000058        15825 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDMM010000058 DACDMM010000000
VERSION     DACDMM010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.058"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            83..1108
                     /locus_tag="IWD16_001410"
     CDS             83..1108
                     /locus_tag="IWD16_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9088475.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(1703..5356)
                     /gene="rpoC"
                     /locus_tag="IWD16_001411"
     CDS             complement(1703..5356)
                     /gene="rpoC"
                     /locus_tag="IWD16_001411"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP9088476.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(5435..9052)
                     /gene="rpoB"
                     /locus_tag="IWD16_001412"
     CDS             complement(5435..9052)
                     /gene="rpoB"
                     /locus_tag="IWD16_001412"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9088477.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            9780..10748
                     /locus_tag="IWD16_001413"
     CDS             9780..10748
                     /locus_tag="IWD16_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9088478.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            10766..11671
                     /locus_tag="IWD16_001414"
     CDS             10766..11671
                     /locus_tag="IWD16_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9088479.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            11668..12474
                     /locus_tag="IWD16_001415"
     CDS             11668..12474
                     /locus_tag="IWD16_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9088480.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            12631..13599
                     /locus_tag="IWD16_001416"
     CDS             12631..13599
                     /locus_tag="IWD16_001416"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP9088481.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(13916..14440)
                     /locus_tag="IWD16_001417"
     CDS             complement(13916..14440)
                     /locus_tag="IWD16_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP9088482.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            14727..>15825
                     /gene="scm"
                     /locus_tag="IWD16_001418"
     CDS             14727..>15825
                     /gene="scm"
                     /locus_tag="IWD16_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9088483.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTST"
BASE COUNT         4575 a         3378 c         3058 g         4814 t
ORIGIN      
        1 ttcaaaatca tagacaaaaa agaatgtcta ttgtactatg gaattattct aaaacgctcc
       61 caaaaaggag gtgcatataa aattgaaacc tactatttat gacattgcga aaaaagctaa
      121 tgtctcaaaa tcaactgtct ccagagtatt aaataaccag ccaaatatct ctgataaagc
      181 aaaagcaagt gtacttcaag caatcaaaga attgaattat gaaccgagca aaattgcaag
      241 aggactgtca ggaaatggtt ttgacgcgat tctagcatta tctcatcgta cttctcatac
      301 gacctatggc aatccttttt tttcagatgt tattcaatcc atttctgccg tttctgaaca
      361 aaataacttc gacttgattc tacaaacggc aaaaacacca gaagaagagc tagaaaaatg
      421 cgcgaaaaag atccaagaaa aaatgatccg aggaattatt attttaagct ctacgatgga
      481 tgaaacactt ctttttgaat tggatcaata taatattcct attgtggtaa tcggaaaagt
      541 cggaaacgac tacacgaaca tctactcagt ggacacggat aatttccagg atagttacga
      601 tttggtggaa acactgatca agaatggaca ccgtcatatc ggctgtctcc atgctccgac
      661 agaagtttat gtatccaatg atcgtctcaa tggttaccgt caatgcttat ttgatcacca
      721 cctagatatc cgtaatgaat ggattataga cggcggcttt cgcttagaag atagcatggc
      781 agcaatgaat gatttaatga agctacgaga aagaccaact gcgatttttt ctaccgatgc
      841 attaaaatca ttgagtattt acaaagtggc agaacaatct ggaaagcaga tacctgatga
      901 tctttcgatc gcaggattca gtgatccctt attgtcctcc ctattttcgc cacagttgac
      961 tcggatagac attccgacaa atcagttagg aatcaaagcc acagaacttt tattccaact
     1021 aatccaccac caataccctg aagaaaaaag aatcattctt tcaaccgaga ttactctcac
     1081 agattcgatc caacaacaag aaatgtagaa cagaattata aaagaacaga aacactgagg
     1141 ataagtcagt tactctgttc ttttttcaat cactcatccc tcgttacccc ctcccggtat
     1201 tttagcgaat ttttcttatt ttccatctgt tttagatagc tgtggataac tataatgttt
     1261 gtgttaaact attttttcca cagggaagaa aaaactattt ttaaccacgt tgtgtataag
     1321 tttttatagt tcgttttttt aaacacattt acccttattt caatccatgt tatttgggga
     1381 tacttatctc taaaacataa atagaataac agacatgtct actggttttg ttttattcga
     1441 ggaattattc aataatagct agcaaaatat taaaaataac cgctctatct tcccatcaat
     1501 ttattttgca ttcaaaaaat gatctatctt tacttttgtt gattgacttc tcttggaaga
     1561 tgatcgttcc gataccttaa ttggatacga accgatgttg ttaagatcct ttcccacttt
     1621 tcttctagtt agacaaatta ttttagacaa accaaaaaag aggttgtgac aataatttgt
     1681 cacaacctct tttttaaaat atctattttg tttgattgtt cattgcgtct tctgcagcca
     1741 tttgcgcttc aatatctgaa atactgtaga cattttcgct tgctacaccg acttcttttg
     1801 gttccatgtc acgataacgt tgcataccag taccagctgg gatgatctta cctataataa
     1861 cattttcttt caaaccaaga agaggatctt tcttaccgcg gatcgcagca tccgtcaaga
     1921 cacgagttgt ttcttggaat gatgcagcag ataagaaact atttgtttct aatgatgctt
     1981 ttgtgatacc tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag actagcgtat
     2041 tgtagttttg atctttaaag tctgcgatgt ctaataatgt acctggtaag atatctgtat
     2101 cacctggatc cattacacga actttacgca gcatttgacg aaccattact tcgatatgct
     2161 tgtcgccgat ttctacccct tgcatacggt aaacacgttg tacttcacga agcaagtagt
     2221 tttcaacaga taatacatcg cgaacttgca acaattgttt tggatcgatt gatccttctg
     2281 tcaatggtgc accacggtgg atcatatcgc cttctgctac tttcatacgt gctgtataag
     2341 gaacagtata cgtacgtgta tctgtttttc ctttgattgt tacttctttg gtacgtgttg
     2401 caggatcttc tgagatatcg atgacatccc ctgttacttc tgtgatcact gcttgccctt
     2461 tcggattacg tgcttcaaag atttcttgga cacgaggaag accttgagtg atatcgtctc
     2521 cggcaacccc acctgtatgg aatgtacgca tcgtcaactg agtaccaggt tcaccgattg
     2581 attgagcggc gatcgtacca actgcttctc cgacttcaac gtcagagcca gttgcaaggt
     2641 tgcgtccgta acaatgtttg cagacaccat gttttgtatt acatgtgaag acagaacgga
     2701 ttgtcactgt ttcgacacct gcatcaacga tttgttttgc gatgtcttcg gaaatcaatt
     2761 ggtttcctgc aatgatcaat tcatttgttt caggatgacg tacttcttta cgggtgtaac
     2821 gtcccactaa acgatcttct aatgattcga tgatttcatt tccctcgcgg atggcttgga
     2881 tttcaagacc tcggtcagta ccgcaatctt cttcacggat gattacatct tgggccacgt
     2941 caaccagacg acgagtcaag taacctgaat cggcagtctt caaggctgta tcggtcatcc
     3001 ctttacgagc cccgtgagtt gagataaaca tttccaagac ggatagtcct tcacggaagt
     3061 tcgaaatgat tggcaattcc atgattcgac cattaggagc ggccatcaaa ccacgcattc
     3121 cggcaagctg tgtaaagttg gaaatgttac cacgagctcc agaatcagac atcatgaaga
     3181 ttggattttt cgcttccaaa ccttccatca gtttttgttg gatgctgtct tttgcagcat
     3241 tccaaacagc aatgacacgt tcataacgtt cgtcatctgt gatcagacca cgacggaatt
     3301 gttttgtaat agtttcaact tgtttatggg cattttcaat gatttcttgt ttttcattca
     3361 gtacactgat gtcagcgatc cctactgtga taccggcatg tgtcgaatgt ttgtacccta
     3421 agtctttcat acggtcaagc atcttagaag tttctgttac tttgaatcgt ttgaagactt
     3481 ctgcgatgat gtttcctaag tttttcttct tgaacggtaa tactaattct tgctctttga
     3541 tatgtgcagg aatatcagta ccagcttcaa caaagtattt gtccggtgtt tggacggtca
     3601 agttgaagtc tgttggttca ttcagataag ggaattcagg aggcatgatc tcgttgaaga
     3661 tgatcttacc aactgttgtg atcatcgtac gttctttttg ccattcagta aatggttttt
     3721 cacctaatga gttagggttc acaccgatac gtgaatgtaa atgaacatat ccgttgcgcc
     3781 atgcgataac ggcttcgttt tgatctctga agaccattcc ttcgccttcg cgtccttctt
     3841 cttccattgt caagtagtag ttacctagaa ccatatcttg agaaggagta acaactggtt
     3901 taccatcttt agggttcaag atgttttgag cagctaacat caacatacgt gcttctgctt
     3961 gtgcttcttc attcaatgga acgtggacag ccatttggtc tccatcgaaa tcggcattat
     4021 aagcttcaca tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa actggttcga
     4081 atgcttggat accaagtcgg tgaagcgtag gtgcccggtt cagcaatact gggtgttcct
     4141 tgattacgtc ttctaaaaca tcccatactt cgtcttcttg acgttcgatt ttacgtttcg
     4201 catttttgat gtttgaagct agttcgcgtt gaacaagctc acgcatcacg aatggtttga
     4261 ataattcaat ggccatttct tttggaagtc cacattggta catcttcaag aaaggtccaa
     4321 caacgataac ggaacgacct gagtagtcga cacgtttacc tagcaagttt tgacggaaac
     4381 gaccttgttt ccctttcaac atatgagaaa gagatttcaa tgggcggtta cctggtccag
     4441 taactggacg tccgcgacga ccgttatcga tcaatgcatc tactgcttct tgcagcatac
     4501 gtttttcatt ttgaacgatg attccaggtg cattcaagtc taacaaacgt ttcaaacggt
     4561 tattacggtt gattacacga cggtaaagat cattcaagtc acttgttgca aaacgtccac
     4621 cttctaattg aaccattggg cgtaaatctg gtggaataac agggataacg tccataacca
     4681 tccagcttgg ttgatttcct gaagcgcgga atgcttctaa aatatccaaa cggcggattg
     4741 cacgtgtacg tttttgtcct tgtgcagatt tcaattcttc tttcaattcg gctgcttctg
     4801 catctagatc aacattatca agtaattgtt taatcgcttc tgcacccata gcagcttgga
     4861 attcttgtcc atattgttca cgtttttcgc gatattcgcg ttctgtcaac aattgttttt
     4921 tctctaaatt cgtatttcct ggttcgatga ctacatatga agcaaaataa atgatttctt
     4981 ccaaagcgcg tgggctcatg tctaatacaa gacccatacg tgaaggaatc cctttaaagt
     5041 accagatatg tgaaacagga gctgctaatt caatatgtcc catgcgttca cggcgaactt
     5101 tcgaacgagt aacttccact ccgcatcgat cacaaacgat tcctttataa cggatacgtt
     5161 tgtattttcc gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc tcacagaaca
     5221 acccttcacg ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt tttacttcac
     5281 cgtaagacca gcttctgatt ttttcgggag aagccaaacc tatttgcata ctttcgaatt
     5341 tatttacatc gatcaaaagg ggttccctcc atttcattta atggcaaacc gcaagacggc
     5401 tgatcttcga ccgccttgca gctcatagac cggattagtc taaattatct tctgcggttt
     5461 caaagttttg gatgacatcg tctttttcat cttccacttg ttcagcagct ttcttttcta
     5521 attctttggc agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg atcaaatcgt
     5581 cgtcttcatc gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc aagacacgca
     5641 tgtccagacc tagtgattgc aattctttca ccaatacgcg gaatgattcc ggtacacctg
     5701 gttttggaat tggttcaccc ttgacgattg cttcgtatgt tttcacacgt ccgactacgt
     5761 catctgattt gtatgtcaag atttcttgta aagtataagc cgcaccgtaa gcttccagtg
     5821 cccatacttc catttcacca aaacgttgtc caccaaattg agctttacct cccaatggtt
     5881 gttgtgtaac caatgagtaa ggtccgattg aacgtgcatg caatttgtca tcgaccatgt
     5941 gagcaagttt gatcatgtac atcacaccga cagacacacg tccatcaaat ggttcacctg
     6001 tacgtccgtc gtacagaaca gttttcgcat cgctggccat accggcttca cgaactgttt
     6061 cccatacgtc ttcatcactt gcaccatcaa atactggcgt tgctacgtgg atacctaact
     6121 gacgtgcagc catacccaag tgtaattcca atacttgccc gatgttcata cgtgaaggca
     6181 cccctaaagg attcagcatg atatcgattg gtgttccatc aggtaggaat ggcatatctt
     6241 cttccggcat aatacgggat acaacccctt tatttccgtg acgaccggcc attttatccc
     6301 cttcatggat tttacgtttt tgaacgatat aaacacgaac taacatgttt acgcctggtg
     6361 acaattcatc tccagcttca cgagtaaaga ttttcacatc gtgaacgata ccgccgccgc
     6421 catgtggtac acgtaatgat gtatcgcgga cttcacgagc tttttcgcca aagatcgcat
     6481 gcaatagacg ttcttctgca gataattctg ttaccccttt tggcgtgact ttccctacaa
     6541 gcaaatcgcc atcttggact tctgcaccaa tacggatgat acccatttcg tctaaatctt
     6601 ttaatgcgtc ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt cctaattttg
     6661 tatcgcgggc ttctgattca tattcttcaa tatggataga agtgtatacg tcatctttta
     6721 ccaaacgacg gctcatgatg atcgcatctt catagttgta cccttcccaa gtcatgaaac
     6781 caactaaaac gttttggcct agagccattt cgccttgttc catagatggt ccgtctgcca
     6841 gagtatcccc tttttcgact ttctcaccca aatgaacgat tggacgttgg ttgtagcttg
     6901 ttccggagtt tgaacgacgg aattttgtta catcgtattt atctaatgca ccgttgtcgc
     6961 ggcgtacgcg gatttcagaa gcatcaacgt attcaacgac accgtcatgt ttacataaca
     7021 aagcagctcc tgagtcatgc gctgatttgt attccatacc agtaccaacc catggtgctt
     7081 gaggattgat caacggcact gcttgacgct gcatgttggc acccatcaag gcacggttgg
     7141 agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact tgtttaggtg
     7201 atacgtccat gtagtctact ttatcaatag atacttctaa gttttcactt tgagcacgag
     7261 ccataacaac gtcttgagca aatgttccat cttcattcaa tgggctgttt gcttgggcta
     7321 cgatgtaatg gtcttcgata tcagctgtca agtagtcgat ttggtcagta acacgacctg
     7381 tttcacggtc tacacgacga taaggtgttt cgataaatcc gaatttattt actttcgcat
     7441 aactggacaa actattgatc aacccgatgt ttgggccttc aggagtttcg atcggacaca
     7501 tacggccata gtgggagtag tgaacgtcac gaacttcata accggcgcgg tcacgagtca
     7561 atccgccagg tcctaaggct gaaagacgac gtttgtgtgt caattcgcca agagggtttg
     7621 tttgatccat gaactgtgac aactgagaag aaccaaagaa ttctttgata cttgccacaa
     7681 ctggacggat attgatcaat tgttgaggtg tcaatgtttc agtatcttgg atcgacattc
     7741 tttcacgaac cacacgttcc atacgtgcta gcccgatacg gaattggttt tgtaaaagtt
     7801 caccgactga acggatacga cgatttccta agtggtcgat atcatcgaca ttgccgatac
     7861 cttccatcaa gttcaagaaa tagctcattg aagcaatgat gtcggctgga cgaaccgttt
     7921 ttacagctgc ttctggatag ccgtttccga tcacgtttac ttcacgttca gggtctcttg
     7981 gtgaaaatac tttgatgact tggacagtca ttggatctgt gaccacaccg tcttctgatg
     8041 gatagtaagt cactgaattt aggccattat caatgaaagg agctagtgtt tccatgactt
     8101 ggtgagtcag aactgttcct ttttcaacga tgatctcgcc tgtttctgga tcaactaatg
     8161 tttcagctaa tgtcagattc aatagacgtg ttttcaaatc taattttttg tttactttgt
     8221 aacgaccaac gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca ttcagcaagt
     8281 tacgtgagct gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa acgtctttca
     8341 aaccttcttc tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg actgtattgc
     8401 gtaatgtttc gctgtcgccg aagatttcaa agatcgtatc atcggaacca aatcctaacg
     8461 cacgaactaa tacggttaaa ggaatcttac gtgtacggtc gatacgtaca taagagatgt
     8521 cttttgcatc agtttccatt tctagccaag ctccacggtt agggataaca gtagaaccga
     8581 atccttcttt tccgtttttg tcgacttttc catggaaata gacacctggt gaacggacta
     8641 actgagaaac gattactcgc tctgccccgt tgatgatgaa tgttccttgt tctgtcatta
     8701 atgggaaatc accgaagaat acttcttgtg ctttgatttc gccagtctcg cggtttgtca
     8761 aacgcaatgt tacatgtaat ggtgcagagt agtttgcatc atgcgcacga gcttctgcta
     8821 ctgtatactt tggttctttc aattcataat ctacaaattc caaagataga ttgccattga
     8881 agtcatcgat tggcaaaatg tcttcaaaca tttctctaag tccctcatct aagaaccatt
     8941 ggtaagagtc tgtttgaatt tcgatcaaat ttggtaattc caacacttca ctgattcttg
     9001 cgaaacttct acgttcgcga tgttttccgt attttactac gtgtccagcc aagctcttca
     9061 cccctctaaa atgtcattca aaaagactac ccgatcaatt gttacaataa tatttcaaat
     9121 attattttta ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa aaccaaaaat
     9181 agaacattct cttgaaacct tacttcgtga gcttctactt ttgtttcctt atattaaagc
     9241 cgaaacggac aatatttcgt ttctgccttt tcttcatcag atagtttttg caacttcata
     9301 tactacactt attttctata aaagtcaaca cttcctggcg tttcttctgc tttttcgcaa
     9361 cattttctta atgttttcaa ttttatgttt tcaattatct gacaattctt aattgacaga
     9421 cctttttatc tcggttatac tttagaaaat tcattttgtg gaggaacttc tatgcgaacc
     9481 aacatcaacc aaatcgcacc aacacaactg aataactttt actttagcta ctttagatag
     9541 gtttgtattc atcatgatgg atacaaacgt taaacgacag tttgtatcta tgatggctaa
     9601 agaactttgt attgcagcaa aaagccacgt agacaaaatg ctaagtggct gagcagtgaa
     9661 aatacaaagt aaaagtatca agcattctac tttttcaatt tattttccga cttttcaatc
     9721 catttggaca ttttgtctga atggattttt tgtttcccaa aattaaggag gaaaaagaaa
     9781 tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct actagcttta ggaggttgcg
     9841 cacaaaaaga caattccgca gaggcatcag atggacaaaa aacaattggc gtattgcaac
     9901 ttgtacaaca taactcgtta gatgctgcct ataaaggatt caaagaagga ttagcagaag
     9961 aagggtataa agaaggcgat aatctaacaa tcgattatca aaatgcccaa aacaatcaag
    10021 acaacctgaa aagtatgagt gagaaactcg tcaaagaaga tcctgatttg ctacttggaa
    10081 tcgccactcc agcagcacaa tctttattaa atgaaacaac cactatcccg atgactgtca
    10141 cagcggtcac agacttggaa caagcccatt tagtcacatc caatgaaaaa cctggcggca
    10201 atgtgactgg cacgacagat atggtgccaa tcgaaaaaca aatcggctta ctgttaaata
    10261 tcgtgccaga cgccaaaaca atcggcatca tgtataacaa cggtgaagca aactctaaga
    10321 tccaagctga tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc aaagtactga
    10381 cagcaaattc cacaaatgat gtccaacaag tgacgaaaag tctagctaaa gatgtagatg
    10441 gaatctatat tcctactgat aacacgttcg caagtgcggc aagtgttgtc ggtgaagtcg
    10501 caaaagaaac aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa gacggcggtc
    10561 tagcaacagt aggaatcgac tacgaaagct tgggcaaaca aacagggatc atggcagcaa
    10621 aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat gatttggaac
    10681 tgtatgtcaa taaagacatg gccaaagcat taggcatcaa tccagacagc atcaaagaag
    10741 ctgaataaaa cacaggagga ttctcatgac aaatttgctc tctcttttct tatcatctgc
    10801 atcgcaaggg gtcttatggg ccctacttgc gattggtgta tacgtcactt tccgtatctt
    10861 ggacattgcc gacttgactg ctgaaggaag ctttccttta ggtgcaggga tcgcggcagt
    10921 aagtattaca aatggctatc atccattgac agcttgtctt ctaggctttt tagggggtgc
    10981 ggcagcaggg cttgtttccg gactgcttca cacaaaattg aaaattcccg cccttcttgc
    11041 agggatcatc accatgactg gtttatattc tgtcacatcc cgtgtgatgg gtgcgccgaa
    11101 catctcttta ttgggtcagc caacgatctt tcattgggtc gagtcattcg gcataacaaa
    11161 agacaacgct gtactgatcg tcggtctgct ttttgctttg atcgttgtat gtttacttgt
    11221 tttgttctta cgtacagaaa caggacttgc tattcgtgca actggcgata atctggcaat
    11281 gagcgaagca aacgggatca acacagataa tatgaagatc atcggttata tgatttctaa
    11341 tggatgcatt gctctttctg gctccttact tgcgcaaaac aacggctttg ctgatttaaa
    11401 ttctggtatt ggaacgatcg taattggact tgcttctatt atcatcgcag aagttttgtt
    11461 acgtaaccag ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg tcatttatcg
    11521 ttttatttta gcgttagtct tcgaactgaa tgttgaacca agtgattcga aacttgcttc
    11581 tgctttcgtg ctagtgatct gtctttcttt cccgcagatc cagcaaaaat tccatttacc
    11641 tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct taacattgga aaatctccat
    11701 catacctttg aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat taatctttcc
    11761 ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa atctactttg
    11821 ctgaacagca tcgccggtac attttctgtc gatcagggtc aaattttatt gaatggaaaa
    11881 aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt attccaagat
    11941 cctaaactag ggacagccgt tcgtttaacg gtagaagaaa atcttgctct tgctatgaaa
    12001 cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac aagaccgaac atttttcaaa
    12061 gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga aatcggtttg
    12121 ctgtctggag gacaacgtca agctattacg ttattgatgg ccacattaca gcgtccagaa
    12181 ctgatcttat tagatgaaca cactgcagcg cttgaccctg ctacttccaa aacggtgatg
    12241 gaattgacag aaaagctgat cactgagcag aatctcactg cttttatggt cactcatgac
    12301 atggaagacg cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg aaggatcgtc
    12361 gtagatatcc agcaagagga aaaacagcag ctcacagtgc cccaattatt agcgttgttc
    12421 aaacaaaaca gcggcacggc attaaaagac gatcaagtac ttttatcttc ataatcagaa
    12481 agaggatggg acaaaaaatg aacgcccacc ctcttctttt ttctctttcc ttctttcgtt
    12541 tttgctttat gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc cttcttcctg
    12601 atagaatgga tgggaaagga gcttgatgcc atgacaacga aagacaaggt acttcaatta
    12661 ttgaaaaaga caccagattt tctttccgga gaaaagttgg cacagcaact ggaagtttcc
    12721 cggacaagta tttggaaagc catcaaagaa ttggaaaagg caggctatcg ttttgagcac
    12781 gggcctacag ggtatcgtta cctgccctct gacgtactag atgcaaaaga aatccagcaa
    12841 gagctacggt cgtttattcc tgatttgaaa gtacaaacga tgttgacttc tgaatctacg
    12901 atgaaagatg ccaaactggc agcagctcaa ggagattctg tttcgaagtt atttgttgct
    12961 gatacacaag aacaagctcg tggacgtttt ggacggcctt ttttcgctga acctggacga
    13021 ggaatctaca tgagtctctt gctccatcca gacaaacggt ttgatgaatt gcctcaatat
    13081 acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg tcgttctgct
    13141 tcgatcaaat gggtcaatga tatctacatg gatggaaaaa aagtctgtgg gattttatct
    13201 gaagcagtca gtgatatgga aagcggcagg atcagtcata tcatcattgg gatcgggctt
    13261 aatttttctg ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc catctctgtc
    13321 tttcccgacg gacaagctag tgtcacaagg aatcagctaa tcagcaaaat ctggcaagaa
    13381 ttgttcactt atctttctca cccagaaaaa gcgttggact tctataaaga aaaatcattc
    13441 gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg tcttgcaaaa
    13501 gaaatcggtg atcatggcga actgatcgtc caaacaaatg aacagaagat gagtttgtct
    13561 tctggcgaaa tcagtctcac atctatccgt acgcaataat tatcagattt cacaaaagga
    13621 cacctgatct catacagtgt ccttttttct gaattctctt caaaagactc tccttattct
    13681 atcagctgac agtttctcta tcctctagaa aaaggtattc taaaaatatc tataaagcat
    13741 ggtacacttt ctttaaatga atcaatgcaa aaaacaagag agtcatcagt ttacacgatc
    13801 aaaagagata gcaggccgct tcctttttca atcttataga tagaccatgg gtaacgataa
    13861 aaacaaaaaa gaacttgcga cttattttgc cgtaagttct tttttagcta gtgccttaat
    13921 ctcttaatcc caacaaacgt ctcgcataga tcacctgacc agagtgcata gcagcatcat
    13981 caataataga aacgagacgc attcctcgtg tgactggtgg tgtccagttc tcgtcaatta
    14041 cttcatctag ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa tcagcagctc
    14101 ctcgcaagta tcctagcaga tcttctattt gattagcttg gatctgcttt gcttgttcca
    14161 gactatgctt ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg ctttcccagt
    14221 cttgactatg ccaaattgga gaagttcctg ctaaatcagc aatctggaaa tctaattctc
    14281 gagcggtatg ccaagccagc caagtcattg atttgatctg cggggctcta tcttccactg
    14341 gaaaacgatt ggcctcctca atagagacgc cttcaaacaa acgttcaaac cgatcgtatg
    14401 cccgatccac actctcgcat agcatatcta aataatccat ttattttcct cctttttatt
    14461 tatcctactt actttttcct aattctagga tacctactta tatgttacta cacaccctta
    14521 ttttttacac tttaacagtc accatcatca atagtttgga gattgaaaga aaaagatagt
    14581 gtcgaactat caattgaaca agaagcaaca atatgaacgt ttcattaata ttctttaaat
    14641 aaacaatgat ttttcaaacg aacagtttaa gactaggaga aaaagagtat aataggaaaa
    14701 tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta gtcctagttg
    14761 ctttgttgtt caatatcttt tcgtatggtg tggccgtttc agcggaaaca tacattccta
    14821 aagaccctgg gaatatttat aatgatctaa ctggtaacta tggcttgtta ggaattgcta
    14881 gtcaattcca cgtatttgct aaaaataaga caactgtgaa tgcacataca gacggaaata
    14941 tagcaacaca tgaacttgat gcaaaaaata attttggtac agagcttcat gaaggtttat
    15001 tgaaccaaga aataaattat atacaaaaaa ctgattcttt cgctaattct tcaggcatac
    15061 ctacaggtag cacaagaatg aataaatttg tcgttggcag taacgtggat gtagcacttc
    15121 aagatggtca tcctacagtc aatggatcca ggcttgatca cttaactcca gatgattttt
    15181 ataaagatcg tgctgggcat gaatatattg atttcgagca ggaatttgct aaattgaatg
    15241 tagcagcaaa tgatcttgca acgatcactc cagctaaaac atatactgct gcagattttc
    15301 cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg tttttattgg
    15361 taaatatcga tgcagaagtg ttaacgatga atacgccact tcaaatcatt aatccaaatg
    15421 atcaagtagt tgtatttaat gtcatcaaca gttcttctgc attgaacgta cagtcaccaa
    15481 tcaaatacaa tgatcgttca aatcatgaaa cagaagattt ttcggatgcg aatatctctt
    15541 ggaattttgg aaatgagatg actgatttga caatcagtgc tccttttcaa ggaacaatcc
    15601 ttgcgcctaa tgctactatc cgcattactc aaaaccaaga cggaacgatt ataggaacaa
    15661 atgtgatttt agatgcagct accaatcgtt gggatcctaa cgaaatattt atcagcgaca
    15721 atggtacaga tacgacagac acaagcgaca gtacagatac atctaccaca agtgatagct
    15781 cagattcatc aacttcaagc gacagtacag atactacctc tacca
//
LOCUS       DACDMM010000059        15752 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDMM010000059 DACDMM010000000
VERSION     DACDMM010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.059"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(512..878)
                     /gene="ssrA"
                     /locus_tag="IWD16_001419"
     tmRNA           complement(512..878)
                     /gene="ssrA"
                     /locus_tag="IWD16_001419"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1049..1822)
                     /locus_tag="IWD16_001420"
     CDS             complement(1049..1822)
                     /locus_tag="IWD16_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9088484.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1966..2316
                     /locus_tag="IWD16_001421"
     CDS             1966..2316
                     /locus_tag="IWD16_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9088485.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2297..3013
                     /locus_tag="IWD16_001422"
     CDS             2297..3013
                     /locus_tag="IWD16_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9088486.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3013..4461
                     /locus_tag="IWD16_001423"
     CDS             3013..4461
                     /locus_tag="IWD16_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9088487.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4531..5040)
                     /locus_tag="IWD16_001424"
     CDS             complement(4531..5040)
                     /locus_tag="IWD16_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088488.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5030..5755)
                     /locus_tag="IWD16_001425"
     CDS             complement(5030..5755)
                     /locus_tag="IWD16_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088489.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5978..8098)
                     /locus_tag="IWD16_001426"
     CDS             complement(5978..8098)
                     /locus_tag="IWD16_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9088490.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8328..9506
                     /locus_tag="IWD16_001427"
     CDS             8328..9506
                     /locus_tag="IWD16_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP9088491.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9522..10280
                     /gene="aroD"
                     /locus_tag="IWD16_001428"
     CDS             9522..10280
                     /gene="aroD"
                     /locus_tag="IWD16_001428"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP9088492.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10303..10788
                     /gene="ybaK"
                     /locus_tag="IWD16_001429"
     CDS             10303..10788
                     /gene="ybaK"
                     /locus_tag="IWD16_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP9088493.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10859..12112)
                     /locus_tag="IWD16_001430"
     CDS             complement(10859..12112)
                     /locus_tag="IWD16_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9088494.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12230..13579
                     /gene="gdhA"
                     /locus_tag="IWD16_001431"
     CDS             12230..13579
                     /gene="gdhA"
                     /locus_tag="IWD16_001431"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP9088495.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13691..15037
                     /locus_tag="IWD16_001432"
     CDS             13691..15037
                     /locus_tag="IWD16_001432"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP9088496.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15345..15731)
                     /locus_tag="IWD16_001433"
     CDS             complement(15345..15731)
                     /locus_tag="IWD16_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9088497.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5147 a         2802 c         3167 g         4636 t
ORIGIN      
        1 tctattattg gaatccttat ttctgcaatt attacacaac tacttcttgt gtttgaagag
       61 aagtcaaaaa ataattaaag caattatgcc ttttagtaca ggaattatta cttcttacaa
      121 atgtatagcg aatgtgaagc ctgctgtttt aattgttcta gaaaaacttt tgagtgaaca
      181 attattttat gttaggatga attaatttct ctagagttta taattaaaaa acttatttga
      241 aggaacatgc ttaaaccata attgtgtttt tccttccatc agcataaaag ttcacctcaa
      301 aaagagtgta cgaacaaaag ttctttttgt aaaccttaaa atagattttc ctgaatacgt
      361 attggggatt tctaaatgtt tgaatgtttt tttgaatgca aaatcaatgc aaaaaatttt
      421 gtatttctct acttttctct ccttgaccaa aataaaaaaa cgttgattta acaacgtttt
      481 tcatacatgt ttatctgtct caaccataca tatatggaga cggcgggagt cgaacccgcg
      541 tccaaacaca tcgccactta aaaatctacg ttcatagtca actcatttaa ggattcactt
      601 tacagcttgc cgagtgacag gcattctgta tcgctagtct gatgttctct ttttcaactt
      661 acagacggaa agttgaaacg tatcccacta aagttaggac ccttactcga gcacatgggc
      721 gatgccgaga ggatctacgc taactgtttt taggcagcta aagcgtaaga gttgttttcg
      781 tttttagcag ttatatttaa ctgtaacgtt tttacgtaga cgtaacctac gaaacgcaat
      841 tcaagctcga ctgtgcctgt cgaatccgta acgtccccgt tagctaacaa gggttacgtg
      901 tatttttcat acaaaattag tatagcatat attgggataa attcaataaa aaagcgaatc
      961 atatcccatt atttatataa taactgcccc tttcttaaga aaaaataagc gattttctta
     1021 agaaaagagg tagcttcaaa aaaagatctt aaatcagatc aaaatacttc aatgcgtgga
     1081 caattccgcc ttctgtattt ttctttgtga taaaggtaga aagttctttt aatccgtcat
     1141 aggcgttgcc catagcgatt ttgttgtcgc aagcttccaa caaggcaaaa tcattcggac
     1201 catcgccaaa tccaaaagta ggaacttttt ccagctgaag ttctttttgc agacgtttga
     1261 cacctgcccc ttttgagatt cctttcttca ctgtatcgat cgagtaagga ccattccgat
     1321 aaaaagttag ctcaggaaaa tgttcgtgat agtattcgtc acctgattga gataagatca
     1381 gcaacatatt tactgtgttt tctttaaagc ctaaaggatc aatttccgga acatctgaat
     1441 gaatgaagtc gtaagcattg atcattgttt ggctatgacc ggtacaccag attttctgat
     1501 cgttatagta agaaagttca tgaccgttca actgtacatg atcatacatt ttttgacatt
     1561 cttctgaaga aaatgtttga gaatagattt cttcgccgtc tactcgaatg aaagaaccat
     1621 tcatggtgac cgcagaagta atgcccgcat cttccatgat agagtggatt tctgcttctg
     1681 tacgtcctgt agcgatgacg gggagaacat tgttttcttt taatgcgttc atagcttttg
     1741 ccacttcggg tgtgattttt gatttcgcat ctagtaatgt tccatcaaga tcaaagaaag
     1801 tgattgcacg ataatttgac acaaataaaa ctcctttttt attggaataa gtttatctta
     1861 acaaagaatg aacaaaagac aaacctttcg tgaagaattc ctgaacatat tggttcagtc
     1921 aacaatttag agtatactat aagaagagaa tttaggaggg aaagaatgat ttttttcagt
     1981 attggtatct tattcttaat aattggcagt ctttttcgga ttctgccctc aaaaggaaat
     2041 ctgccttttt atgggtatca ttcgcctctt gccgcaaaaa cagacgctca ttggcggctg
     2101 gctcaaaaga caagcggaaa ctggtttttt cttatgggct tactgatggc attgatcggc
     2161 tattatttaa agacatctgg gcatacgaat tattttctga ttgaaatgct tctgctagta
     2221 tttccaatta tgcctatttt tattatgaca gaaaagaaat tacagaaata tgacctggaa
     2281 actggaggga acgataatga atattttaat gattgaagat aatcaatctg tttctgaaat
     2341 gatgcagatg tttttcttaa atgaaggttg ggaagcaact ttcaagtatg atggaaaaga
     2401 agggctcgat gcctttttag aaaacccaca acattgggat atgattacac tagacctgaa
     2461 tttgcccaca ctggacggta tgctagtagg aagagagatc cgcaaagttt caagtactgt
     2521 gccgattatc atgctgacag ctagagattc tgaaagcgat caggtgatcg gcttagagat
     2581 gggggcagat gattatgtca caaaaccttt cagtcctctg acgcttattg ctcgaatgaa
     2641 agctttgcat cgccgtagtg agttggcaga agcaagagaa gaagcagcag tttcagatga
     2701 tcattttgat atcgagacta gtcatttcaa gatgaatacg aaaacacgag aagcgtatct
     2761 ggacaaccgg ccaattgaag gactgacgcc taaagagttc gatctattgt ttactttagc
     2821 aaaaaaacca cgtcaagtat tctctcggga gcagttgctt gaattagtct gggattatca
     2881 gtactttggt gatgaacgga cagtggacgc ccatatcaag aaattacgac aaaaaatcga
     2941 aaaggtcgga ccacaagtca tccaaactgt ttggggagtc ggctacaaat tcgatgattc
     3001 tggtgttgcc taatgcgtta tttgtatcag caactcttag ctttttgcgg aatggtcgcg
     3061 ttgatcttat tgatcgttgg agtttctttt acacaactga caaaacaaac gattgaagaa
     3121 aacaattacc agcaactgtt tggatatgct gaatcagttg aaaagacaac gcaaacctat
     3181 gccgatacgc tcccgcagtt tagtcaagat caggcgtttc aaaatgcatt gctgctgaca
     3241 gagcaagtac tgacagaaca aaatgtgaac tttatattta tcgataaaaa cgaacgtgtc
     3301 atttatccga ctaatacagc tttactgaat tttaccttaa cagctgatca atggaaaaat
     3361 ctgaggaatg gacgtcaaga aaaattcaca tcgaataaaa atattttagg acaagcacag
     3421 gcgacctctt acgcacttgt gccatttaat ttgaatcatg aattctatgg agcacttgta
     3481 gttagtcaac ctgcgcgaaa tatcgataac agcgttcgtc caattacact gaacctgttc
     3541 aaaggattta ttttttccag tatcgttgca gttattgcca gctatgcttt tgcagcattc
     3601 caagttaagc gtatcaatcg gttaaggaat gcgacgaaag aagtgacgaa tggtaatttt
     3661 gatgtacagc tccccgtaca tgacaaagat gaatttgatg agttagcaga tgatttcaat
     3721 aaaatgacga attcattgaa agaatctcaa gcagaaattg aagaacaaga aaatcgtcga
     3781 agacaattta tggcagatgc ttctcacgag atgagaacac ctcttacgac aatcaacgga
     3841 cttctggaag ggttagaata caatgcaatc cctgaaaatc agcgagaaaa cgcgattaaa
     3901 ttgatgaaaa atgaaacaga gcgtctgatc cgtttggtga atgaaaactt ggattatgaa
     3961 aaaatccgta cgaatcaaat cagtatggtc attaaaaaat tcaatggaac agaaacattg
     4021 aaaaatattg ttgcacagtt agaagctaaa gcagaagcag caggagatac attgacattg
     4081 aaagccgttg atgatattga tgtttacgct gattatgacc gatttgtcca aatcatggtc
     4141 aatattattc aaaacgcgat acagtttact gaaaacggcc aaatcacgat tactttagaa
     4201 aaaggctatc ttgaaacgat catcacgatt gaagatacgg gaattggaat gtctgaacaa
     4261 caaatgaaaa gtatctggga tcgttattat aaagtagatc cttcaagaaa aaatacaaaa
     4321 tatggtgaat caggattagg tttaccaatc gtgcagcaac ttgtacgctt gcataaagga
     4381 aaattagaag tagaaagcga actaggaaaa ggaacaaaat tcgctgttcg tttgcctgac
     4441 gtagaaatca aaaaagagta aaaaacaagc ggtataaaag atcatgaatc gatcttctat
     4501 accgcttgtt tttactataa aatgattgat ttagcccgcg ggctctaaac gatcaatata
     4561 aggactttga gcaatagtag catcatgagt aaagagtaag ataccttgat tttcttcatg
     4621 agctaaataa gaaagcaagt ggagtagctc ttggatctcc aatacactaa gttcatcaaa
     4681 tgtatcttca ataacaatat tgtttcgctt ggaaagcaag gcatgaagta attgcagctt
     4741 tactttttca aaagacgtca actcagctgc cgcacaatta agcaaggaag gagagatatc
     4801 aagtttttct tgataggcca aacgatcttg tttgatttct ttttccggta catccaacag
     4861 gacatggtct ttaagattca gataaggaaa catcggccaa tttttttgga tgatcgcaaa
     4921 attatccagt tttgcaacaa gaacctcttt tttcaagtgc tccaatattt tatgctgttt
     4981 ttcgagagga cacaaaataa cggtcaagtg ttcagcatga atcaaatatt caggtttcat
     5041 tacgtccagg cctcctactt tgcaaatgat ggataaaaaa agtgaaaggg aatagactaa
     5101 gaatgagaca gcatccactg agcccaataa gagtttgggc aaatccctta agtatatctg
     5161 tgaaaaaaag atcattttgg aaatcgatgg taaaaaaagt ctgttgatcc atcggaattg
     5221 taagtccgct taccgaagtc aagatatcac ggatatctgg cagttcagta gtctttaaga
     5281 caagaaaatt gatcctttga agaagcgctt cataaactga ctggaagctt acgatccaca
     5341 ataaaaccaa gatacaacaa agaaagagag gaaagaacag ctgccagaaa ataaaaagga
     5401 ataatttggt tttagaaaga ccagtcaggc gccaattgat caattcctgc ttgttttgcc
     5461 tgactgaata gtaaaagaaa aagataaaaa ggaaggcaaa tagaaaaaat aaactgaata
     5521 tgccttttat ataaaatcgg ctagctaatt ctaaagccgg aagaagctct gtagatgcag
     5581 aagcgccaat aaatgaaagc cgatcttgga cttgattggc taaagccgat tgcaaatggt
     5641 aaagagtcag gcatgctatc aataaaaaag cagaaaagct tgaaaataaa caatagagta
     5701 ttgtctgctt tttgtaataa attaatcgag cagtagcata gcggattatt ttcatacttt
     5761 tcacctctat aaaaagtata gaaaatgaat atgaataaag tatgaaattc ctttagtgaa
     5821 atcacgaatt aaaaccagaa aatattttgt aaaaagcaag aggttgtgaa gaagctgccc
     5881 tattctatta ccgtagatgc aagcttttga acactgttta ttcgaaaaga ggagcagcga
     5941 caataatttt gtcgctgctc cttttaaatt tattttgtca ttcggaagaa cttgtggtcg
     6001 ttccgtgaag ttcttggtac gtttgcgttt tataaagatc aacagttgat tgtccgttgt
     6061 gttcactaaa gacactagtt gacttgtcac caagagattt ttcagcttct tttaatttct
     6121 ctaagccatt cttgtagtca taatcactgg tatcaattgg gtctaatcca ctattcgtat
     6181 agaaccgaag aagatcacca ttattgattt ggtcagaaac agctaactgt ttatcaactt
     6241 tttctttcag tgaatcaatt tcattctgta cttcctcagt aggttcagta atagcttcac
     6301 cagtttcgtt actatagatc gtaccaccgt agtaagtata ttttggtgta acaaaatcac
     6361 catcacggaa agaaacaagc tgttcatgat ctttcgagaa tagatcttgt cctaactgaa
     6421 tataattttt tgtatctaca cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc
     6481 cgccgtaagt gtgattgatt ccgccctgtg tttgtccagg gatatggatc atataaggga
     6541 cacgctgcat ttgtgcattg tcgtagtttg tccaagtaga acttgttttt ccaactaatt
     6601 cagctagatt tttatttctt gaattcgaga caccgtaatg gtcaccgtaa aggacgatga
     6661 ctgaattatc atacaagccg ctttctttca aataattgaa gaattcttgt acggcggtat
     6721 ctaaataatt ggctgtcgca aagtaaccgt tgatcgtttc atcatttgta ttggctaacg
     6781 ggaatcctgc ttcgtcattg gtgaattttg aataaggata atggttagat acagcaatga
     6841 attttgaata aaatggttgt tgcagacgtt ccaagtattg tacggattgt tggaaaaatg
     6901 gtttatcatg caacccgtat tggaacgaat tgtcatcatt aacatcataa taagaagaat
     6961 caaagaaata atcatatcct aaattcttgt atgtctcgtt tcgattccag aatgtaccag
     7021 cgttcccatg gaataccgca ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag
     7081 cttggaaagt attttttccg cctaattgtg tgaacaagga gccttgattc aagccaaaca
     7141 atgaattttc catcagcgtt tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa
     7201 agttgtcaaa actgaacgtg ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt
     7261 catgctggag accattctca tctgtcaatt tataatcgat caggaattgc tggaagcttt
     7321 ctaaatggat ataaatcaca ttttttcctt tagcgagccc gaacaaatcg tcatttggtg
     7381 ctgcataatg ttcttttaca taattttcta cttctttcat atcattggga cttgcttctg
     7441 cccgcacttg gtttgtctgg taagtttgaa caccgtcata aaccatgaaa gcattcaagc
     7501 caagatattt gaccaaataa tcacgagaga atgttcgagt cagcaattct ggacgatcag
     7561 cttccgccat aaaaagattt cctgagaaaa tcataactgc taatgtagag atcgcaaaag
     7621 ccattcttgc tcggatcggg cgagggtcca acttgatttt ttttgcaatc agcagaacag
     7681 tcaagataat aaaatcgatc caatagatca catcatatgg gcgaaacagt cgaatggcac
     7741 tttctcccag accactcgct actttcccag cacctaaaat cgtgttgatc gtaatgaagt
     7801 ccgtgaattc tcgataataa gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa
     7861 tgaccatcat cgtgatataa gcaactctag ggcgtctgat ataaagagca attccaagta
     7921 ataacaatgt agttgcaata gggttgataa acaagataaa gtattgaatg ccactttcga
     7981 ttccgagatg gaaatcaact aaataggcaa aaatcgtttt caaccaaagt aaaacgacta
     8041 gtaaagaaaa aaatccgagc ctcttattca aaaaggcagg tgtttttata catttcaaga
     8101 taatcttcct ttctgcactt cttaatcaca gaagtgtcat gaaaccatgt tacacagctc
     8161 acttattctc atttatttta ctagtatgac aatatagtgt caattgaaac cagaagttta
     8221 tgctgtttca ttaaggtttt ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct
     8281 atgttttttg ttatacttgg gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag
     8341 taaaaaaaag agcaagcaat cgattgaaaa aaggatatcc actgatccaa aaagaagacc
     8401 ttgctcatcc ccaaaaagaa accacgcaat gggtggaatt agttgattcg caaaaccaat
     8461 ttatcgcaaa aggatatcta ggaaagcaga ataagggaat cggttgggta ttgagccagc
     8521 agaacatccc atttgatcaa tctttttttg aagaattttt tgtgaagtca aaagaaaaaa
     8581 gaactttctt tttttcagat gattctacta ccgcttttcg tatattcaat ggagaaggag
     8641 acggaatcgg cggtctgatt gttgattatt atgcaggtta tgctgttttt tcttggtata
     8701 acgaaacgtt atatgcacat cgtgaattat tgattcaagc ttttcgtgaa atctacccgg
     8761 aaatcgtagg aggctatgag aaaattcgat ttgaatcagt aaatctgcct gagagccagt
     8821 tcatttacgg aaaagaggca ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg
     8881 ccgtttattt aaatgaagga ttaatgactg ggatcttttt agaccaaaaa gaagtacggg
     8941 gatcattagc agaaggtttc gcttctggga aaacagtttt gaacatgttt agttataccg
     9001 gcgccttttc tgttgctgcc gcaatgggag gggcaatcga aacaacaagt gtcgatctag
     9061 caaaacgcag tttaaaaaaa acacaggaac agtttgaagt gaatggtttg gatgtcgatc
     9121 agcaaaaaat cattgttatg gacgtattcg agtatttcaa atacgccaaa agaaaacaat
     9181 ttttatatga catgattgta ttggatccac caagttttgc cagaaataag aaaaaagtct
     9241 ttcgcgtagc aaaagattat ggggaactgg tcaaggatag tctggatatt ttagcagaag
     9301 acgggatact tattgcgtct acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa
     9361 tgattgaaga ggaattccat gccaaaaatg tttcttttga acaacttgct ttatatcgtc
     9421 tgccaaaaga tttccagacg acggatacct ttttagaagg caattatctg aaagtgtttg
     9481 tttatcaaat taaaaataac cattagaagg agcgatgcag aatggtttgg gtaaagggat
     9541 tgaatatagg aagcggtaga ccaaagattt gtgcgcctct tatcggggcc gtccgagagg
     9601 aagtattacg tgaagcagtt cttgcacgtg aagcaggggc cgatttagtc gaatggcgag
     9661 tcgatcatta tcgtgaagtt ttttcttttg atgaagtagc agaaacattg gttcttatcc
     9721 atgagatgct tgatggactg ccgttattat ttacgtttcg taccttagct gaaggcggag
     9781 aacaagacat atcgatccgt cagtatcgcg acctttatga acatgtgata cacacaggat
     9841 tagtagatat ggtcgatatc gagttgttca aagtagaaag tctaggaaaa agactgatag
     9901 aggaaatcaa gtctttaaac attcctttaa ttatcagtag tcatgatttt aaagaaacac
     9961 cagcagaccc tgtcttgctt taccgattga atgttatgga acattttgga gccgatatcg
    10021 gaaaactggc agtaactccc aataatgaac gagatgtttt gcggttgatg gagttgacta
    10081 gacgggcaaa tgcttttgtc tccatgccta ttattacgat gtccatggga aatcttggta
    10141 aaatttctcg tctggctggc ggaacgacgg gttccgttat gacatttggt tccttaagca
    10201 gcgattctac ttctgctcca ggacaattac cggtcaaagc attgaagcaa attattcaat
    10261 tattaaatga accgttgtag aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata
    10321 gtaaaaacga atgcaatccg tatggtcgaa caaaaaaaga ttccctatac agagcacgaa
    10381 tatgagtggg acgaaagcca cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc
    10441 caatctcgta tatttaaaac actggttgct gtgggcaatg tgacggggcc tcttgttgct
    10501 gtgataccgg gagaagccga attgaatcta aaaaaattag caaaagtttc agggaataaa
    10561 aaagtagaaa tgctccattt aaaagatttg gaagcaacga ccggatacat acgtggtggc
    10621 tgctcgccta taggtatgaa aaaactattt cctacttatt tagatcaaat tgcagaaagc
    10681 tatgaacaaa tcattgtctc ggctggcaga agagggctac agatggagtt agcacctcaa
    10741 gatatctgtg cattaacgag cggacaattt gcagatataa aacaataaat catacgtata
    10801 aaagaaagaa ggaggccggg atgttgtttg tccagtctcc ttctttcttt tttatacatt
    10861 atgctctttg cccggcattc attcctgcta catgccctgt agcgaaagcc gctgtgatat
    10921 taaagccgcc agtataacca ttgacatcca gtaattctcc cgcaaaatac agaccatcaa
    10981 tgattttact ctccaatgtt ttaggataaa tttcctttag agaaacccct ccgccagtta
    11041 caaatgattt ctcaatagga aaagttttgt tgattgatag cttaaactct ttgcatagtt
    11101 gaacaaaatc ttgaatttgt ttttgagagg cttgttgtcc tgtcagatga tccatttcaa
    11161 gccgctcaag aaagaaaact aacaatcgct caggcaatat accatgccaa gcattgacca
    11221 agttcttttt ggttgcttta cttttttctg tcaatatatg aataagctct tgtttggttt
    11281 gtgtagggaa acaatccaga gaaagtgtca ctggttctcc cgttttctct aattctttat
    11341 tgacaaaact tgaacaacgc agtgcagccg gaccagaaat accgaaatgg gtgaaaagta
    11401 aatccatcgt atgttcagtc aatacacgac cttttttatt taaaacgcgt aaagtgatgt
    11461 cttgtaaaga aagcccttga agtgttttct cctgaatata cggttcatcc gatatcaagg
    11521 gggactctgt cgcataaaga ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc
    11581 catctccagt agaaccagta gatggataag tccgtccacc agttgttaag ataatggcag
    11641 gagcatggaa ctcttcgaat tccgtgcgta taccgtaaat tttatgttct ttgtggatca
    11701 atttctctac tcgagtagag aatagaagcg tcacatctag ttctttcaga cgattgagca
    11761 atgcttcgat aatcgttttt gatttatttg ttacaggaaa catccgtcca tgatcctctt
    11821 cttttaaatg tacgccttgt gattcaaaga agttcatgat atcgaaatta ttccattgcg
    11881 caaacgtact atataaaaac tttccatttc caggaatatg tgcaatcaaa tcatcaacag
    11941 gacgattgtt cgtaacattg caccgaccac cacctgtcat cagtagtttt tttcctgctt
    12001 ttttgttttt ttcgatcaag agtacgcgtg cgccttgttc agctgcactg atcgctgcca
    12061 tcatcccgct tgttccagcg ccgactacga tcacatcata cagtttttcc attccctcac
    12121 ctcaaggaaa gtgtagcata tctgaagaaa gaaaacagaa aaaaaatgtt ttgagccaaa
    12181 agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga tgacaaacgc
    12241 aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag ggcaagaaga
    12301 atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg acactcatcc
    12361 cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag ttattcaatt
    12421 tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt accgtatcca
    12481 atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta gtgtcaatct
    12541 aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa ccggtctgcc
    12601 aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg ataatgagat
    12661 catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc catcgattga
    12721 tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt tcggcgaata
    12781 taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg atttttgggg
    12841 aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca aacatctatt
    12901 gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa gcgggaatgt
    12961 agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta catgttcaga
    13021 ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga aagaagtcaa
    13081 agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg ctatttatca
    13141 tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc catgtgctac
    13201 tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga tcaaaatcgt
    13261 agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc atgaagcagg
    13321 cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat ctgctttaga
    13381 aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg agcagctgga
    13441 tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt acggcgacga
    13501 aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca aagcaatggc
    13561 agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc attttgttta
    13621 tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa acctataagg
    13681 aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt ttgtcaacga
    13741 acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat tacgggaagg
    13801 tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact atgacaaaga
    13861 agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg aagtattagt
    13921 tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact tcttgaatca
    13981 cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct tctttgccgg
    14041 aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg atcgcgactt
    14101 ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg cgttccgtgt
    14161 attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac gtatctacgc
    14221 tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag gttgggaaac
    14281 tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg ttggtttgtt
    14341 accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg ctgatgcaag
    14401 caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg cagcaatgcg
    14461 caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg aaccaggaat
    14521 gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa aagaccaaaa
    14581 aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag gacaattcat
    14641 ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac ctcgtaaatc
    14701 aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc aaggaaaaga
    14761 agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata cagacggtga
    14821 cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg gttatacaat
    14881 gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca acccattcga
    14941 ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca aaccaggctt
    15001 tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt cgctagattt
    15061 aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc atttaggtac
    15121 tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt tgtcacagtc
    15181 ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta ggactttaag
    15241 aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa tcagtaagca
    15301 aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga taggcctaat
    15361 ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc tatgatccgc
    15421 ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc attggcagag
    15481 tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc agcataagca
    15541 tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt gatatcagtt
    15601 tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata agtagactga
    15661 tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat caaccctaaa
    15721 atttttttca tgaaagatct ccttttttat tt
//
LOCUS       DACDMM010000060        15728 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDMM010000060 DACDMM010000000
VERSION     DACDMM010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.060"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            343..2403
                     /locus_tag="IWD16_001434"
     CDS             343..2403
                     /locus_tag="IWD16_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088498.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2486..3055
                     /locus_tag="IWD16_001435"
     CDS             2486..3055
                     /locus_tag="IWD16_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9088499.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3230..4036)
                     /locus_tag="IWD16_001436"
     CDS             complement(3230..4036)
                     /locus_tag="IWD16_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088500.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4174..5688
                     /locus_tag="IWD16_001437"
     CDS             4174..5688
                     /locus_tag="IWD16_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP9088501.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(6091..7074)
                     /locus_tag="IWD16_001438"
     CDS             complement(6091..7074)
                     /locus_tag="IWD16_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP9088502.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(7079..7726)
                     /locus_tag="IWD16_001439"
     CDS             complement(7079..7726)
                     /locus_tag="IWD16_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9088503.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7811..8443)
                     /locus_tag="IWD16_001440"
     CDS             complement(7811..8443)
                     /locus_tag="IWD16_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9088504.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8459..9010)
                     /gene="coaC"
                     /locus_tag="IWD16_001441"
     CDS             complement(8459..9010)
                     /gene="coaC"
                     /locus_tag="IWD16_001441"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP9088505.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(9007..9744)
                     /locus_tag="IWD16_001442"
     CDS             complement(9007..9744)
                     /locus_tag="IWD16_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP9088506.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9741..10730)
                     /locus_tag="IWD16_001443"
     CDS             complement(9741..10730)
                     /locus_tag="IWD16_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9088507.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10988..11530
                     /locus_tag="IWD16_001444"
     CDS             10988..11530
                     /locus_tag="IWD16_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9088508.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11547..12632
                     /locus_tag="IWD16_001445"
     CDS             11547..12632
                     /locus_tag="IWD16_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9088509.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12644..13450
                     /locus_tag="IWD16_001446"
     CDS             12644..13450
                     /locus_tag="IWD16_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9088510.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13451..14278
                     /locus_tag="IWD16_001447"
     CDS             13451..14278
                     /locus_tag="IWD16_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9088511.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14275..15348
                     /locus_tag="IWD16_001448"
     CDS             14275..15348
                     /locus_tag="IWD16_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9088512.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         5089 a         2817 c         3178 g         4644 t
ORIGIN      
        1 tgggaatatt ttggaaatac catcatcaga gaaaaaaata gacatttaac tgaaaaaatc
       61 agctagtatt tatggatggc agctttgtct cacatattta ttagctgatt tatgtttttt
      121 tcaaagaaaa ggtaactaga tataactaga tataactaga tatattactt aacaaaatta
      181 aaaagtacat aagttattat tccgattttc ttgaaacaat aaaaaatcaa aaaaaatact
      241 ctcctaatag tccatcttct tttgatttta tttatataat gaaatcatct tatgaaaatt
      301 actacttcat gtttagacaa gagtagggag gactaagaat aaatgtgttc aaaaagacgt
      361 tcaaaacaac taaataaaca cgcttgtcgt ttagataagg aacggtcgtg ttctgaaaaa
      421 cactacgtta agtttatgat gcgcacgatg ctgatcagtt tatctgtagg tgtctatatg
      481 catgtacccg ttcaggcaac gacacaaacc atcaaggtcg tacgtacctc tgtttctgag
      541 accactgatt cccctatcac tattactcat actttcactg atgcaaatgg aaaacagcta
      601 cttcctgcat tgacttccta taataaagtc agttatcctc caacgataaa ccactactgg
      661 ttaattccta acaaaacatt atggacatcg gaaaccggaa gcagtgtgcc gctttctatc
      721 ttttccgata tgactgatcc tacaaaaggt gatcagttaa aacagatcac tgatattttg
      781 aatcatgctt tgacagatat aaaggacggt ggaagcgcac aagtttctta tgtatatgaa
      841 aaagattatt cacagtttga aagaaagaaa atcgatgttc tggcagggac aaaagtttcg
      901 atatctgatc tggtagaaag gctgaagaac gcagatggaa cacaaggaga tatgtccaaa
      961 gtaatttcaa acaatgggaa tatcgtggat acgactaata caggaacatc tactatttcc
     1021 ttgtcctatt ttgatccaat tgctttaaaa acaatgactg catctgctgt gatccatgtt
     1081 cgtgctgatc aaacagctat caaaggaaaa gatcttcact taagaacagg agagagttac
     1141 gcccctaaag atcttgttga atatgtaaca aaaagcgatg gaacttcagg aaataaagaa
     1201 gaaatcaaaa tgaacgggac agtagatact acaactccgg gagaatatcc cgttgtatta
     1261 acttatatag actcgaaaac atcaaatgtg gcagaaacaa cagctgtagt ttatgtagaa
     1321 gcatctgctg cttcatcgac tgtcatcact cattttatag atagtcaaac aaataaagaa
     1381 cttcaagcag aaacacatat gagtagtgta aatcgtattc ctgatttgcg cctacctgct
     1441 gaattttctg atcgagtgcc aaatatagat ttatcgactt atgaacaaaa tgggaaagtc
     1501 acttctattt ctgattggat gcaagcaaac ggattagaca gaaaaagtca ctggtcggag
     1561 atccttgaag tacttcactc agaactgatg aataatagga agcaatcagg aggggaaatt
     1621 acctttactt atgtttatat accagacaat cgttcattag aaggtcatga tcttattgtt
     1681 catccaaacg aaaaaataaa caaagaagag ttgatccaat cagcaacaaa ttccttaggt
     1741 aaacccgtac cgttaaagga tgtatcggtt acaatcaatg aagtaccatt ggaggatggt
     1801 tatttgacca aacaaacagg tgattttacg attatgtata gttatcatga cccgtattca
     1861 ctccaagaac tccatgctca gtcactatta catgttttac cattaaacaa acagatagat
     1921 caaaaagaac aaaaaagacg agaagaaagc ttgtcagaaa aaaaacaaca aatggctcat
     1981 gcagtttctc caacagggaa agtaaaacaa acaagaagcc tagagttagc aagaatatct
     2041 agaaaaatct cagaatctaa taaaacgata aggcaagaag ctcatttggc cccacaagtt
     2101 tctggattgg aaaatgaaat tttagagcct aaaataaaac aacaatcaga aaaagaattt
     2161 gttgctaaac agtcggaaga tcagaaggaa tcagatgacc agaaggaaac gaagaaaaaa
     2221 cgcttgtcaa ctattgattc caccgatgag catctgcagc cgcaagtacc acttcctagt
     2281 ccgggaacac ccgcacctgg tggaggtatt ccttcttctg ctttaggaga tttcatgcga
     2341 ggtatggcag gaacatgggt atacgctaat cgaaatgaat ttgacgcaga tgactggttc
     2401 tgatttatat ctctatctgt tatagtcaaa taagtagctg agtcatatgt tatttggcat
     2461 aatggagacg aggagggata gaaaaatgtt agataaaacg ttaccttacg cagaaatctg
     2521 gatgacaaga ccacttcaac aaaatgttcc aatcacgcct ttagcggaag gttttgaatt
     2581 tcgattttat caaaaagaag atgaatctgc ttgggcggag attgagactt ccgttgggga
     2641 atttcaaacg acagatgaag cacttgagta ttttcaacaa aactttgctc cttatcctga
     2701 agaacttgaa agaaggatgt tttttgtaga gacaaaaaaa ggagaaaaag tagcaacctg
     2761 taccgcttgg cgaaaaaaat caagagagca agtgtatccg gtttttcatt ggttagcagt
     2821 gaaaccagat taccaaggaa aagggctcgc aaaagcattg actgccagag tcttgcaaga
     2881 atttcctacc ctccatacgg acggaccaat ctatcttcac acacagacat ggagccatca
     2941 ggcgattgct ctttataaaa aaatgggttt tacttttatt gctgaaaatc tagacggcac
     3001 acctaatcca gattatgaaa aagttatgca ggttttaagt aaattagata aataacgaaa
     3061 tggtgaaata agtcctcagt attgggataa aaaacgaaat agtcattcct catatcttac
     3121 ttttttgagt taatactatc ggataactaa tggcatgcca tacattcaaa aaagaagggg
     3181 cagtgacaaa agtattgtca ctgccccttc tcttgttttt tagcatgttt tatcgtttga
     3241 tttttttagg aataaaattc ttgatgaacg taaatttttg gtaatcgttt cgactataga
     3301 ggtaaccaaa tacactgaaa acaactgctc caataaaatt aacaaataaa tccttcatag
     3361 tatccatgat tcccacgtct aagtaaccat tttggatgat tgttttatct gttttaccat
     3421 ctgcattttg actttcaatg attgtccgag tgatatctgg aacatgatag acagtgtttc
     3481 cgtctttact taactccaca ctattgattt gctggatgat cgtatctttt tgcatatcga
     3541 attgcagtaa tttatctgct gaaaattcca caaactccca cataacacca actgtcatcg
     3601 aaaaacagaa ggaaacaact gctagtaata aaggagtcaa gttcatggtt tttgtatttt
     3661 tattgagcaa ataaacaagg gaaaagccga cacccccagc taaaaatccg ctgaatgcat
     3721 gtagaaaagt atcccacatt gaaaaatagc cataaaaatc acttaactct cctaaaactg
     3781 ttgaactaaa aataaaagat aacaaaatga tttctagcat atttggaatc tgtatcttta
     3841 atatcttttc cagaaaataa gggatttcaa ataaaattaa tgtcagtaat aaaagcattg
     3901 tatttcccca gtgttggttc cagacctgat ccactagcat aataacgacg atgatacgta
     3961 ataaaatata gattccgttt gtgattctac ctgcagtttt tgtactgttc gtaaaaaagg
     4021 ttttcaaatg attcataaaa taactccttc atgtatatcc ttaattatac taagaagcgt
     4081 gtgtttacca aagctatatg acttaaagaa aaacagagtt gatattgtga aaaaaatcac
     4141 ttaatatagt ataaggagaa aagggggata caaatgaaaa aattagttgc tggtttatta
     4201 acagccgttg cacttcttgc tatgagcgca tgcggcgcag atcagaaaaa tacagcgcag
     4261 acaagttcta gtacgaagga atctgccgaa gtatcatcag gcgcttctga acaagtatat
     4321 acagatccaa aagaaatgaa agaatcttat gatgtcgtga ttattggagc tggtggtgca
     4381 gggatggcag cagctatcga agcaaaagat gctgggttga atccagtaat tttggaaaag
     4441 atgcctgttg ccggaggaaa cacatctaaa tcatcaagcg gtatgaatgc ttcagaaaca
     4501 aaatttcaaa aagaagaagg aatcaaagac tcgaatgatg ccttctatga agaaacgcta
     4561 aaaggtggtg gaggaacgaa tgataaagaa atgcttcgat actttgtcga tcactctgct
     4621 gatgcggtcg attggctaga tcaaaacggt atcaaactgg acaacttaac gatcactgga
     4681 gggatgagtg aaaagaggac acatcgtccg tctgatggct cggctattgg tggatactta
     4741 gtggaggggt tattgaataa cgtccatgag cgggaaatcc ctatttttgt caacgcagat
     4801 gtgacagaca ttaagaaaaa tggggaaacg gtcaatgaag tgactgtaca tgttcaagga
     4861 gaagaaccaa aagaggtcac cggaaaagca atcgtagtta ccacaggcgg ttttggtgcc
     4921 agcaaagaat ttattgaaaa atatcgtccc gatttagctg attatattac gacaaatcaa
     4981 gaaggatcaa ctggagatgg tatcaccatg attgaaaaat taggtggaca aaccgttgat
     5041 atggataaaa tacaaattca tccaactgtg cagcaggatg agggggtctt gattggtgag
     5101 gttgttcgcg gagaaggagc aatcttggtc gatcaagagg ggaaaagatt tgtcaatgaa
     5161 atggatacaa gggataaagt atctgcagca attacagcat tgcctgaaaa atcagcttat
     5221 ttgatttttg accaaggcgt gcgtgatcgt gccacagcta ttgaatttta tgaccaaaaa
     5281 ggatatgtca caactggaaa gacaatggaa gaattagcag aaaaaatcga tttaccaaaa
     5341 gaaacactag caaaaacagt tgatggatgg aatacggatg ttgcggagaa aaaagatgct
     5401 caattcaacc gtgccacagc catggaacat gacttatcgt ctccgaatta ttatgcgatc
     5461 aaaatcgcac caggaatcca ttacacgatg ggcggtgtga aaatcaatac gaacactgaa
     5521 gtattagata aagaaaacca gccaattagg gggttatatg ctgctggtga agtcacaggt
     5581 ggcttacatg gaaacaaccg gatcggtgga aattcagttg cggagatcat tatttttggg
     5641 cgacaagcgg gcatacaagc agctcgattt gcttcggaaa atgaatgaaa tagaacgtaa
     5701 ataaaaagag gttgtgaaat cagcgttttg agctaatacc gagggtcagc tgattgaaca
     5761 ctgtttagtg aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt
     5821 aaaaacagct tcttatgttt ttaacttggt tggacttatt accgcagatg caagcttttg
     5881 aacaccgttt attcggaaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc
     5941 tctaaacacg tataaacggc ggaagcagaa gaaattcctt cggaaataag ctgaaattca
     6001 caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct
     6061 gtcccagcct cttttttgat cagcatgatt ttattcagtt atcttcaaaa tgatttttcc
     6121 gatgtttttg tttgcttcca tatattcatg cgcttgtttg actgcgctga attggaatgt
     6181 acggtcaatg attggccgaa tcttgccatt ttctagaaaa ggcccagctt tttgcataaa
     6241 agtttgtgcg agctctgctt tatactgatc acttctaggt gtcaataatg taccaagcag
     6301 ttggacacgt ttcccaatca atacaccaag gtctatctca ggcacgatgg tcccacctag
     6361 cataccaatc aagacccaac gtccatcaac ggctatagaa gctaaattct tttcccagta
     6421 agaagcgccg atgaaatcca aaataacatc gactccccga ccttcagtga tttttcgtac
     6481 ctcttcttga aagtcttgtt ttttatagtt gatcaattca tctgcaccaa gttctttaca
     6541 aaaggcgagt ttttcttctg atcctgcagt aacgatgatt ttcgcgtgag aaagctgtct
     6601 agctaattgg atcgctgctg ttcccacacc acttgctcct gcatggatca agacagtttc
     6661 gccttcttgt aattttccaa gccagtaaag tgtttgataa gcagtcaaaa aaacttctga
     6721 gattccagca gcagaaacat agtctagcgt atcagggaga aggattgcac gatcggcagg
     6781 catcacagca tattcggcat aaccacctag attgactaat ccgtatactt ttgtcccagg
     6841 agtaaaagtg gagatatcac ttttattctc aactacctct ccagctactt cgactcccaa
     6901 aatagggtag ggaggtttca aagagactga ttctcttttc ataatatctg ttcgattgac
     6961 agctgctgta tggactttga tcagtaattc gccttgtttt ggtttaggaa caggcacttc
     7021 aatttgttct aatacatcta cagaaccagg atgcttgatt gaaatggcac gcatggatct
     7081 attcctcctt tgcccattct tggaatgctg tattcaactc tttgctacct tcagaaagta
     7141 ctagagtcgt acgagaaata tccgtttctt ccactagagc ggcaagtaca gcagcaactg
     7201 tagagcgagt gatcgttttt gatgccttct cattaggctt agttaattga atggaagtaa
     7261 cttcttcatc gttagtcaat gtgactggtt gaagaatgac aaaatctaag ttagttcgtt
     7321 tcagccattc atctgcataa tgtttagtaa tataataatc tgtcatggac tcaggccatt
     7381 ttgctggttc atctgcataa acagcactga ccatcagata gcgagagaca tgcgcatttt
     7441 cagctgcgat cactgttttg attgcgccat ccaagtctac ttgcagaaga ttctttcctt
     7501 gagagccggc agcaaaaatc aatacatcga tgcctttgaa tgcttcggtc attttttcca
     7561 cggaccaagt taaatcaaaa gagacatacg aaacattctc tttttttact aatgtttggg
     7621 aagcaacatc tctcacccct gcaaaaattt catgggaact tggagctaaa tcttttatca
     7681 ggtgtcggcc aatttgtccg tttgcaccta caacaaaaat tttcatttgg atcatccttt
     7741 cgacgtcaaa tctttccatt caatagtaac accaaaagag aaaaaaatca gagatcggct
     7801 acgatgacta tcagttagta agagaagggt gcttgaagta tcttaacaag acattgcata
     7861 ctgctgcagt cgcaagacct gcgacgattc cttcaggtat accacttgta aaaataaccg
     7921 ctagaatagc tgaataaaca gcatttaaat ttgttccgat gactttggca taatcttgct
     7981 ggaataaaaa gtagatcata ttcatcacta aaatcgtatt ggtcaatgaa ccggaaagtc
     8041 cagctaagaa taaagaaacg gaatctgctt tttgtttcat tagtttgcgc agacctttat
     8101 aaacgaaata aggaaccacc ccaatcaaga tgcgaggaat aaacgcgatc agcaaggctt
     8161 tccagcttcc atgttctgtt ccgatgactg ggataaacgg agaaaagaca aaagacaaag
     8221 gtgtttggat aactgtgcta cggatcatgc tgatcagtcc aaatgtaccg ccaagaaaag
     8281 cacctagtct tggtcctaaa acgatcgaag caatgattac aggaatatgc atggttgttg
     8341 cattgattgg accgataggg ataaaaccta aaggcgtaac agctaataaa atcaaaatag
     8401 ctaaaaacat cgcagttaat gtaaagtttt ttgtattttt cataagtact ccttttttct
     8461 aagcatttct ttcattaaga gtcagcatga cctgttcgat gatcgtctga atatcagcta
     8521 aggcaccttt tccaaaatct ccacaagcta aaagagattc tcttggttca atttcctgat
     8581 agccgacaga tctcaatgtt tccagattac gctgagtgat cgggttttga tacatattcg
     8641 tgttcatggc aggggcaatc aattttggtg ttcccactgg aagcgccatt gcgactgttg
     8701 aaagaagatc gtctgcaatg ccgtttgcta gcttgccgat aatgtttgct gtagcaggcg
     8761 cgatgagaaa caagtcagct tgttttgcta gttcgatatg attgattacc tgcggatttg
     8821 attcttgcat gacgtctgta tggactgcgc gcttcgacag agattgcaag gttaatggcg
     8881 tgataaaatg tgtgctgctt ttagacataa tcacatctac ctgggcacct aatttgacga
     8941 attgacttgt aagatcagcg cttttgtaag cagaaatact tccagtaacg cctagtaaga
     9001 tctttttcat tttgattcct ccagtctaat tttctcagta atcaatgcag caatctcaga
     9061 tttactttga gcttcttcca ctgttccatc tttagataag agatagccgt ggtgtgtctc
     9121 gccatggatt tccgtcaaat catttgcgaa aataaaatca gttttgttat ttatcagact
     9181 attttttgcg actgccagaa gagattcctt tggtacatct actaatagtt tgaaaccaat
     9241 gatttttgct tttggctgcc attcacgtag ataagaaatg atttttggtg ttttcttcaa
     9301 aacgagcaca agatgctcgg tgttagaaga gatctttgaa acagtggtat ctgtctgttc
     9361 attttccgca aaccaattgt ccagttcttc ttgcgttgaa gaagtgggta ggttttttgc
     9421 cagctgttct tctgaaaaac taaaagcagg agtaaaatca ctgactgcca tactgtggat
     9481 aatcgcatcg tagtgttctt ttttcgttag ttcttctaat tgtaaaaata agtccagtgc
     9541 agattcgatc gtataaatcg ttatattgga gtgtcttttc ggtttcagtg cttgtttcgt
     9601 agtcacgtaa tcgattgtgg tatccggatt ggcagctaaa taatcagcta atgcttgccc
     9661 taatcgtcct gtcgaatgat ttgtaatagc acgtacttga tcgatttttt cagtcgttcc
     9721 tccagctgtg attagaatct tcatttatgg tcacctacat ttactagtgt tcttccttgg
     9781 tgtgttccag caagtagttt agatgctgtt tcaggcaatt gatccagagt aatctcttgg
     9841 acgaccagtt catctgcaat ctgcaaatcc gccatctgtt gccataaaga aagacgttga
     9901 tctattggta cattgacaga atcaattcca atcaactgga tgtttcgcag aataaaaggc
     9961 aagacagtcg cgtttagttt gattccccca gcattgccac ataaagctac tgcaccgtta
    10021 taagagatca atggaagaag actgcttagc tgctcgccac ctactgtgtc gatgacatag
    10081 tcgatttgct gttttcctaa agcttttgtt gtctctggca gaaattcatc aggtcgtacg
    10141 acttgcaaag cacctagact ttttagccat gtgacagact cttcttttct ggaaaaggca
    10201 atgattgact ggaatcctag tttatgtaac agggctatcg ctgtactgcc tacaccgcca
    10261 gaagcaccag taacgacaac ttttttatcg gaggtcatac cttgacggat taaagcgttc
    10321 acacacaaca tagcagtaaa ccctgctgta ccaaggatca tcgcttgacg cagcgtcata
    10381 ttttttggta gaggaactaa ccagtctcct ggtacttttt gatattggct ataaccgcct
    10441 ggatgggtga cgccaagccc aaaaccagta gcaagtacaa gatcttcttt tgaaaaacgt
    10501 ggattgttac tttggacgac agttccggat aaatcaatcc ctggagtcat cggatatgtt
    10561 cgtatcacct gaccagactc gctcattgcc aatgcatctt tataattgac atcagaatag
    10621 gcaattttta ccaagacttc attttcagcc aactcagtaa atggcagatc actgattgct
    10681 gtatggaaaa caggttcctt tattacctga aaagatcgaa aagcgttcat tcgtgagaca
    10741 ctccttttaa aattttcttt tatcatacca taaagcctac tcttttattc ttgttcttcc
    10801 gtaaatttag acaaaaatta ggtgaaaaat aaagaatcaa acaactagaa tttattccaa
    10861 aagataaaga aatattcagc aaaaaaaatt ttacaaaggg ttgccttttt caataaattt
    10921 cgtatactaa tacgagtggt agtaaacagg aagtttacta ttagaaaact tcgattcgga
    10981 ggaaattatg gaaattggtg agaagttaag aaatttgcgc atccaaaaga atttgacaca
    11041 agaagaacta ggcgaacgca cagatttgtc aaaagggtat atttcccagt tggaacgaga
    11101 tttaagttca ccatcaatgg aaacattctt ttctattctt gaagtcttag gtgtgactcc
    11161 agaagaattt tttcatcaag aagcagcgaa tctgcaagtg gtttattcaa aagaagatca
    11221 tacggtttat ttcgatgaag aaaacgggta tgaattggaa tggctcgtgg ctgattcgaa
    11281 tgaaaaagaa atggagcctg tcttgctgac ttttgaaggt gcaggagaat ataagacgtt
    11341 tgaaccttca ttatctgaaa cgtttatttt tgtattagaa ggggaattag agttaagctt
    11401 aggagagacg gtctatgtgg caaaagcagg acagtcgatc tattatcaag cgacaaaaca
    11461 acatcaatta aggaatcatc tcaaaaaacg aacaaaagta ttgattgttg caacgcaatc
    11521 atatttgtaa tatagggggt aaaaacgtgg ggaaaccaat catttcattt gatcaagtag
    11581 tgaaacgcta tgatgatgaa acagtattaa aaaaagtaag ttttgagatc gaacaaggaa
    11641 aattttatac actgcttggt ccttccggct gtggtaaaac aacgatccta cgtattatag
    11701 ctggattcac cgaagctaca gaaggggaca tctattttga agggaaacgg ttgaatgatt
    11761 tgccagctaa taagcgacaa gtaaatacag tcttccaaga ttatgcactt tttcctcata
    11821 tgaatgtttt tgataacgtt gcttttggat tgaagatcaa gaaaatgtcc aaatcagata
    11881 tcgaaaagaa ggtaaaagat gctttgcgta tggtccagct tcctggatac gaaaaacggg
    11941 agattagtga aatgtctggt ggtcaaagac agcgtgtagc gattgcacga gcaattgtaa
    12001 atgaaccgaa agtattgcta ttagatgaac cgttatcggc gcttgatttg aaactacgta
    12061 cagatatgca gtatgaactg cgggatctgc aacaacgatt aggaatcacg tttatttttg
    12121 ttactcatga ccaagaagaa gcgttggcaa tgagtgatga gatttttgtc atgaataaag
    12181 ggaaaatcgt ccaaagtggt acacccgtag atatttatga tgagccaatc aatcatttcg
    12241 ttgctgattt tgttggggag agcaatatcg tggacggtgt aatgatcgaa gataatctag
    12301 ttgagtttgt tggtaagcaa tttgaatgtg tcgatggcgg gatgcgtcta aatgaaccag
    12361 ttgaagttgt tttacgccca gaggatttga cgatcacgac accagataaa ggaaaactag
    12421 tcgtaacggt cgatactcag ctgtttcgtg gtgtccatta tgaaatcatc tgttatgatg
    12481 aacagcaaaa tgaatggatg gtccattcaa cgaaaaaagc taaagaagga agcaaagtag
    12541 gattggcctt tgagccagaa gatatccatg tcatgcgttt caacgagtct gaagaagaat
    12601 tcgatgcacg tcttgacagc tacgaagaat aggggggaca agaatgaaaa caatgcgcag
    12661 gatctattcc gtcccttata ttttgtggct cggtttattc gtgatcgcgc ctgtattaat
    12721 gatcatttat caatcttttt ttgatatgaa tgggcaattc acgctgaata attatctatc
    12781 ttattttacg tcggggacat cgctttctat gacgatcaat tctgtatggt atgcattttt
    12841 gatcacgttt ttcacgctga tcatcagtta cccgactgct tattttttaa caaaactgaa
    12901 gcacaagcaa ttatggctga tgctgattat tttgcctaca tgggtgaatc tgctgcttaa
    12961 agcttacgca tttattggga ttttcagtgt gcacggcagc gtgaatcatt tctttgattt
    13021 tatcgggatc gggccgaaac agatattatt cacagacttc agttttctgt tcgtggcaac
    13081 gtatatcgag attccattta tgattatgcc aattttcaat gcattggaag agatgaatcc
    13141 ttctttgatc agtgctagta gagatttagg cgcgaatagc tttgagacct tccgacgagt
    13201 gatttttccg ttatcattaa atggtgtgaa aagtggtgtc caagcagtat ttattccttc
    13261 tctttcttta ttcatgctga ctcgtctgat tggcggaaat cgggtgatca ctttaggaac
    13321 ggctatcgaa cagcatttct tggttacaca aaactggggg atgggttcta caatcggtgt
    13381 gattttaatc attgctatgt tcatcgtgat gctcctgaca ggcgagaaga aaaaaggagg
    13441 tcgctcataa atgaaaaaat ttaaatggtc gtatctttat ttagggatcg tctttttatt
    13501 cttatatgca ccgatcttct atctgatttt ttattccttc aatgaaggag gaacgatgaa
    13561 cagctttaca ggatttactt gggaaaatta tgaagctgtt tttgaagata cacgtttgat
    13621 cattatcgta ttgaatacgt tcatgctggc tttcttatct gcattgatcg ccacgatcat
    13681 cgggacattt ggggctatgg gaatctatta cacgaaaaaa cgacgtgcac ggacagcgtt
    13741 gttgagcatg aataacatct tattagtctc tcctgatgtt attatcggtg ctagtttctt
    13801 gattttcttt acactactag gattcggatt aggttttcag agtgtattgc tttcccatat
    13861 tgctttcagc attccaatcg tcgtcttgat ggtcttgcca aaattgcagg aaatgaacga
    13921 ttcaatggtc gatgcggcta gagaccttgg tgctaataat ttccaagtag tcaaaaatat
    13981 cattttgcct tatctttctc caggaatcat agctggatat tttatggcat ttacctattc
    14041 tcttgatgat tttgcggtta catttttcgt cacaggaaat ggctttacga cgctctcagt
    14101 agagatctat tctcgtgcaa gacaaggaat cagtttagaa atcaatgcgc taagtgcgtt
    14161 agttttcttg ttctccatga tattggtcat tggatactac tttattagta aagaaaaccg
    14221 tccaaaacgg ctgaagaaaa acagaagagt acgcgagggg gtggcacggc tgcgatgaaa
    14281 aaattacaat ctctgttttt aggtattctc atcattattg cggtgctgct gcttggtgtt
    14341 cgtcaacttg aaaaaacaag tggcatgagt ggagcaaagg tacttaccat ctataactgg
    14401 ggagattata tcgatcctag tttgatcacg aaatttgaaa aagaatatgg atacaaagtg
    14461 aattatgaaa catttgattc taatgaagcg atgttcacaa aaatccagca aggtgggaca
    14521 aactacgaca tcgctatacc aagtgaatat atgatccaaa aaatgatcaa agaaaaacta
    14581 gtacttccat tagatcattc gaagatcaaa ggattaaata atattgatcc acgattttta
    14641 gacttggatt ttgatccaga taatcagtat tcgattccat atttttgggg aactttaggg
    14701 atcgtatata acgataaatt ctttgatgca aatcaaatta agcattggga tgatttgtgg
    14761 aagccggaat taaaagacag tttgatgctg attgacggtg cacgagaagt tatggggctt
    14821 tctttgaata gtttaggcta ttctttgaat agtaagaata tggcacagtt gacagaagca
    14881 gcaaataaat tgaatcattt aacaccaaat gtcaaagcaa tcgtggcaga tgagatcaaa
    14941 atgtatatga tcaatgaaga agcctccgtg gcagtgacat tttctggcga agctgccgat
    15001 atgatgtggg aaaatgaaca tttacattat gtgatcccac cagaaggatc caatctttgg
    15061 tttgataata tcgttatgcc aaaaaccgtg aaaaataaag aaggtgccta tgatttcatc
    15121 aactttatgc tagaaccgga aaatgcggca caaaatgcag agtatattgg ttattctaca
    15181 ccaaatgaag aagcaaaagc gttattgcca aaagacattt cgtcggacga acaattctat
    15241 ccatctgatg acacgatctc ccatcttgaa gtctacaaag atctaggatc gaaataccta
    15301 ggcatctata atgacctatt cttagaattc aagatgtata ggagataatg gcaaaactca
    15361 gtaagaatgg ggacttatca acgttctaaa gagctaaaaa tcgtcctttt tctatctgag
    15421 ttttccaatc ttctaccaaa agcggatgaa acgccgcttt ttttgtgtta ttttattttt
    15481 tctaccacgt ttttctacca gagaaaagtg gacaaaacca ttgtaaaata gggcccgaga
    15541 cgggctagta aatctaagat tgaaaaagtt acagaaggat aaagtaattg gaaactatgc
    15601 aaatatactt aacccttctg taatctatta ataaaactaa tatatgaatc cagttcaata
    15661 tttgtagcta tgaaaaaaca taaaaatcat attagataaa aactaaattg gtagataaat
    15721 tattttgt
//
LOCUS       DACDMM010000061        15470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDMM010000061 DACDMM010000000
VERSION     DACDMM010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.061"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            138..1475
                     /locus_tag="IWD16_001449"
     CDS             138..1475
                     /locus_tag="IWD16_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9088513.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="IWD16_001450"
     CDS             1523..3652
                     /locus_tag="IWD16_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP9088514.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="IWD16_001451"
     CDS             3627..4316
                     /locus_tag="IWD16_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088515.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="IWD16_001452"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="IWD16_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="IWD16_001453"
     CDS             complement(5546..5740)
                     /locus_tag="IWD16_001453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088516.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="IWD16_001454"
     CDS             complement(5790..6230)
                     /locus_tag="IWD16_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP9088517.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="IWD16_001455"
     CDS             complement(6234..7079)
                     /locus_tag="IWD16_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP9088518.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="IWD16_001456"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="IWD16_001456"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP9088519.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="IWD16_001457"
     CDS             complement(8703..9650)
                     /locus_tag="IWD16_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9088520.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="IWD16_001458"
     CDS             complement(9873..10223)
                     /locus_tag="IWD16_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9088521.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="IWD16_001459"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="IWD16_001459"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP9088522.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="IWD16_001460"
     CDS             11646..11966
                     /locus_tag="IWD16_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9088523.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="IWD16_001461"
     CDS             11988..12452
                     /locus_tag="IWD16_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9088524.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="IWD16_001462"
     CDS             12657..13262
                     /locus_tag="IWD16_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP9088525.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="IWD16_001463"
     CDS             complement(13301..14590)
                     /locus_tag="IWD16_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9088526.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..>15470
                     /gene="rlmH"
                     /locus_tag="IWD16_001464"
     CDS             15018..>15470
                     /gene="rlmH"
                     /locus_tag="IWD16_001464"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP9088527.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRI"
BASE COUNT         4826 a         2892 c         3173 g         4579 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc
//
LOCUS       DACDMM010000062        14420 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDMM010000062 DACDMM010000000
VERSION     DACDMM010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.062"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(53..988)
                     /gene="murB"
                     /locus_tag="IWD16_001465"
     CDS             complement(53..988)
                     /gene="murB"
                     /locus_tag="IWD16_001465"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP9088528.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1061..1981)
                     /locus_tag="IWD16_001466"
     CDS             complement(1061..1981)
                     /locus_tag="IWD16_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9088529.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2094..2846
                     /gene="xth"
                     /locus_tag="IWD16_001467"
     CDS             2094..2846
                     /gene="xth"
                     /locus_tag="IWD16_001467"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP9088530.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3710..4243
                     /locus_tag="IWD16_001468"
     CDS             3710..4243
                     /locus_tag="IWD16_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP9088531.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4733..5257)
                     /locus_tag="IWD16_001469"
     CDS             complement(4733..5257)
                     /locus_tag="IWD16_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9088532.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5250..5723)
                     /gene="tsaE"
                     /locus_tag="IWD16_001470"
     CDS             complement(5250..5723)
                     /gene="tsaE"
                     /locus_tag="IWD16_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP9088533.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5846..6826)
                     /gene="pta"
                     /locus_tag="IWD16_001471"
     CDS             complement(5846..6826)
                     /gene="pta"
                     /locus_tag="IWD16_001471"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP9088534.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(6979..7659)
                     /locus_tag="IWD16_001472"
     CDS             complement(6979..7659)
                     /locus_tag="IWD16_001472"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9088535.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7785..8654
                     /locus_tag="IWD16_001473"
     CDS             7785..8654
                     /locus_tag="IWD16_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9088536.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8680..9195
                     /locus_tag="IWD16_001474"
     CDS             8680..9195
                     /locus_tag="IWD16_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088537.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9242..9805)
                     /locus_tag="IWD16_001475"
     CDS             complement(9242..9805)
                     /locus_tag="IWD16_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9088538.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9860..10039)
                     /locus_tag="IWD16_001476"
     CDS             complement(9860..10039)
                     /locus_tag="IWD16_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088539.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10116..10625)
                     /locus_tag="IWD16_001477"
     CDS             complement(10116..10625)
                     /locus_tag="IWD16_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088540.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10721..11101)
                     /locus_tag="IWD16_001478"
     CDS             complement(10721..11101)
                     /locus_tag="IWD16_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9088541.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11431..12180
                     /locus_tag="IWD16_001479"
     CDS             11431..12180
                     /locus_tag="IWD16_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP9088542.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12364..13641)
                     /locus_tag="IWD16_001480"
     CDS             complement(12364..13641)
                     /locus_tag="IWD16_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088543.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13720..14364)
                     /gene="pcp"
                     /locus_tag="IWD16_001481"
     CDS             complement(13720..14364)
                     /gene="pcp"
                     /locus_tag="IWD16_001481"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP9088544.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
BASE COUNT         4079 a         2936 c         2582 g         4823 t
ORIGIN      
        1 agcaggacgg ccaccgtcct gctcttttac ttattttaag ttccattact tattaattgg
       61 ctaaacttct gcttgtttct gatattgctt gtttttctcc aatgatccgc acttctgttt
      121 ctaaagacac atcaaatttt tctttgatta cttcttggat gtgcgcaatc aattcaatat
      181 aatctgtcgc tgtcgcatga tcgatattga cgatgaatcc agcatgtttt tcagagactt
      241 gtgcaccgcc ccatttcagg ccttgcaatc ctgcttgttg gatcagctga cctgtatagt
      301 gtcctactgg gcgcttaaag acacttccgc atgaagggta ttctaatggc tgttttgatt
      361 gacgcaattc ggtcaactca tccattcttg ctttgattgc ttcataatcc cctgtttgca
      421 aactgaatcg agcttccaat acgatagcac gtaattcttg gactttactg tgacgataag
      481 aaaataccat ttcgtctttt gtcaaagttt tcaatgttcc atcttcaagc aaaagatcca
      541 cttcagcaaa aacatctttg atctctccgc catacgcacc tgcattcata taaaccgctc
      601 caccgacact tcccgggatg ccgcaagcaa attcaaatcc agtcaatgat tctgccaaag
      661 ctgcataagt cgtatcaatc agtgaggcac ctgcttccgc aatgaccatc gttcctttga
      721 catgaatctg tttcatttca gtcagcatga tgactacacc gcgaatcccg ccatcttgaa
      781 cgatcagatt gcttgcattt cctaatacca tccaaggtat atcatggatt cggcaataat
      841 cgatgatttg ctttacttct tcttgttttt tcgggaatgc aaggacatca gctggtccac
      901 ctgttttcgt aaacgtatag ttcatcaatg gttcatcaaa caatatagta atcgtggcta
      961 agctttcaac tatttcttct ttattcactg aggattttcc tttcttttct caaacgtcct
     1021 cattattcta tcacgcttaa tacaatcttg ccagtcttgt tcaaagttga tgacggtagt
     1081 gatctttaag cacttcctca cataattcat gacttcttac aacttctttt tgtttaagca
     1141 attctgtttc cgatttacct aaacggatct gagaaaagaa ggcctctacc attggctcaa
     1201 acccgcgttt gactagcgtg tttgtccagt cgccaaattc tttcactttt tttccatcag
     1261 cctcttgaat gacaagctgg gtcagatcgt ttacttcata agtaccattt ttacttgtta
     1321 cttgatattt ttctatatta gcgccacttg ctaaatccat cgttaaaata gccgtttgat
     1381 tttgtgtttc taattgtaaa atggcatatt ccaaaaattc ctttgtttct cttatctggt
     1441 attttgttct caaaatgggt tcatctaata aatacacagc tgtatcaata agatgtaaaa
     1501 acaaatcata aaggacgaac tctgtcgttt cttttgccgc tattcgattt ttttgcaggt
     1561 ggatcaaccg cttatctggg atacgtttca attcttctac cataggagca aagcgccggt
     1621 tgaaaccgat catcaaaatt atttggttct tatcagctaa ttcttgcaat tcacgaactt
     1681 catttagatg gacactcagt ggtttgtcaa tataaacatg gataccattt tctagacatt
     1741 ttttggctaa ttcataatgt acttttgttg ctgcgtggat catacatgct tcgatttttt
     1801 cttctaaaag ctcatcgatc gtttcatata gatgttggaa cccgtactct ttttgaattc
     1861 tttcttttgt ttctttattt cttgttgcaa aataaaaatc aagtgttcct tgttttttcg
     1921 cataagttgg taaatatgct ttttctgcga tattccctac accgattaca ccaatcttca
     1981 tctactttcc tcctggatca tttagttact ttcatcttac accatttttt actaaaatga
     2041 ggtacaatag tatcgcaaag gtaaggaaat taattcgaag gagtgtgctt ttattgaaat
     2101 ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa taaaaatttt ttagaagttt
     2161 ttcatgagtt agacgccgat tttttctgtc tgcaggaaac gaaactccaa gcaggacaaa
     2221 ttgatttaga tcttccaggt tattatcaat attggaatta cgcggaacga aaaggctatt
     2281 ccggcacagc gatttttgct aaaaaaccag cattgaatgc cacttacgga atgggtatcg
     2341 atatccatga tacagaaggc cgtctgatta ctttggaata ttccgatttc tttcttgtaa
     2401 cctgttacac gccaaattct caaagcgaat taaaacgttt agattaccga ttagaatggg
     2461 aagaagcctt ttataattat ttggaaaatc taaaaaaaca aaaaccagtc atcgtgtgtg
     2521 gggatctaaa tgtcgctcat caaaagattg atttgaaaaa ctggaaaacg aatcaaaaaa
     2581 atgctggatt cactccagaa gaaagagcgg ctctttctcg actgttggat aacgggttta
     2641 tcgatacatt ccgttatttt tatccaaccc aggaaggtgt ctactcttgg tggaactacc
     2701 gattcaattc aaggaaaaac aatgctggtt ggcggatcga ttactttttg acgagcaaag
     2761 atttagagcc aggactagct gatgcaaaga tccatacgga cattatgggc agtgatcatt
     2821 gtccagtaga attagatttg aaataaagag gttgtgaaaa agtcgtttag tttcgagtat
     2881 taagaaaaat tttgtgaaaa tggctttcca cattttgcat aaatttggca taataccgag
     2941 aaactggctt ttgaacactg tttatcgaga gattgtgaaa aagctgtcct gcatcaagta
     3001 ataagaacag ccaaacaaaa atagttcctc atatttttcg tttggttggg cttattaccg
     3061 cagatacaag cttttgaaca ccgtttatta agaggttgtg aaaaagctgc actgcatcaa
     3121 gtaataagaa cagccaaaca aaaatagttc ctcatatttt tcgtttggtt gggcttatta
     3181 ccgcagatgc aagcttttga acaccgttta ttaagaggtt gtgaaaaagc tgcactgcat
     3241 caagtaataa gaacagccaa acaaaaatag ttcctcatat ttttcgtttg gttggactta
     3301 ttaccgcaga tgcaagcttt tgaacaccgt ttattgagaa gttgtgaaat cagcgtcctg
     3361 acctgagtat tagagaaaca aatggcaaaa tagttcctca tattttgcta ttttttgagc
     3421 taataccgag ggtcagctga ttgaacaccg tttagtggag aagttgtgaa aaagtcgcct
     3481 actttcgagt cgtaagaaat ttttttgaaa atggttcctc acaactttgc ataaatttga
     3541 cttaataccg agaagtcatg atccccatct aactccaaat cacaaggaat gagttcaaaa
     3601 actagctttt ctcaacaaat attacataaa aaattctatg ttttttgtta aatttatttg
     3661 cttttttcaa aagtatgtta cagtaatttc ccaaggaggt tttacagaaa tgaattttgt
     3721 tgccatggat tttgaaactg ccaatcatca gccttatagt gcttgttctc ttgcgctagt
     3781 catggtaaaa aatagccaga tcgttgatga attctataca ttgatacagc ctgagactcc
     3841 ttttttctgg cgaaatgtcc aaattcacgg aatccatcaa gaagacgtac gcaatgcgcc
     3901 gaaattccca gaagtctggc aaactatcca accatatttt caaaaaaatc gtttagtcgt
     3961 tgcacataat gctgcttttg atacaaaggt cttagcggga tgcttagatt attatcatct
     4021 tgaacagcct aattatcttt ctttatgtac agtgaaaagt agccgtcgcc tgtttcctga
     4081 atttccaaat catcgattga acactgtgtg cgaaaatttg aacatccaat tagatcatca
     4141 ccatgatgca ttggaagata gtcgtgcatg tgcggaaatc ctgctttatc aagaaaaaaa
     4201 attcgggaca gatccattga aaaaacttgt gactataaag taaaagaata cttatgaaac
     4261 acacaaagag gtcgtgagcc tgccgtttca ctccgagtca caaggagcaa tttcaaaaaa
     4321 tagcttctca tgtttattaa atattgtgac tggtgaccga ggagctggca ggagaacacc
     4381 gtttattgga gaggttgtga aaaagctgtt ctgcatcaag taataagaac agccaaacaa
     4441 aaatagcttc tcatattttt catttggttg gacttattac cgcagatgca agcttttgga
     4501 caccgtttac caagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaacg
     4561 gcaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgaaggtc agctgattga
     4621 acaccgttta ttcaaaaaaa atggactgcg acacgacttt tgtcacagtc ccttcgtgtg
     4681 tttttctgcc ttctcaaaag gatgacagtt tctcactctt ttcctatttc ttctattcaa
     4741 tcaaataact cattttatat acatctaaaa attcacctag atctgttctt gcccctctag
     4801 gcatgaccgc ttcgatttgg aagcccattt ttcggtaaag gtgtacggct ctttcattac
     4861 gaacttgaac gtcaagctcc aatcgaaaaa gtacacccat ttccttcgcc caactgatga
     4921 tttcttctag cataagagtg cctagtccca ttccccaata ttcctgcaga atactgatac
     4981 cgacttctcc cacgtgagac aaacgaaatt gactgtctgc cttgacagat gctgttccaa
     5041 taatcgtccc ttcatagatt gcaagcagca ataaattatt attgctttcg tatatataat
     5101 ccaactcttc ttctaaggtt tctggtggta acaggagttc tgcttcatcc aaaactaaaa
     5161 attctgtctc cttattaact tgtcccatca tcaaaagaat gtcttttgca tctcctggca
     5221 ctgcttcccg caagatgatc ccaacttctt cactcatgat tttcagccca tttttctaga
     5281 attcgctgtt tgaaggtttc actttgctcc ccgtatgaga ttagtttcac ataccgcttt
     5341 tctaaatctg tatcactttt ttctaaaatc agttccaaat aatcctccgg cagtgcttct
     5401 tctaatagat ttccccattc aacaacggaa agcccatcac cttcaaaata ttcatctaac
     5461 cctaaatctg ctccgctagc tgctacccga taaatatcca tatgatacaa cggaatccga
     5521 ccttgactat attctctaat aatcgtgtat gtcggacttt tgatcatttg cgttatccct
     5581 aatccttggg caattccttt cgtcaacgtt gttttacccg cacctagatc accagtcaat
     5641 atcagattgt cacctggcat tgcggaatga ccaattgcct ctgcgaattg ttctgtcatt
     5701 gttaatcctg atagttcgat catttttcat cttctcccgc tcttctttct ttttgccagt
     5761 atatcgcgta atgaagtttt tttaaagaaa aaatgaacta gtcaagaaca gcagctgctg
     5821 tttttggcta gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct gtgataatcg
     5881 ataatttata tacatcttct tcatttgctc cacgagacaa atcagaaacc ggtttgttca
     5941 agccttgtag aattggtccg attgcttcaa agttaccgaa acgctgagca attttatagc
     6001 cgatatttcc tgactgcaat tctggaaata caaagactgt tgcttgacca gctacttctg
     6061 aatctggcgc tttcagttgt gcaactgaag gaacataaga agcatcaaat tgcaattcgc
     6121 cgtcgatcat gtattgtggc gctagttttt tagcgatttt agttgcttca actactttgt
     6181 ctacttctgg cgatttcgct gagccttttg tcgagaagct catcattgct acttttggtt
     6241 cgatatcgaa caattctgct gtttttgcag aatctacggc gatttcagcc aattcttgcg
     6301 cagttggatt cacgttgatc gcacaatccg aaaagacata tttttcttga tctcttccac
     6361 gtaccatgat catcgctccg cttgtccgac tgatgccagg ctttgttttg atgatctgaa
     6421 gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt ccgtctgcaa
     6481 tccccatata cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc ttttctgctt
     6541 gttctttgga tgcctttcca tttctacgtt caacaaaagt atccaccatt tcatcccact
     6601 tatcgtaatt atcagggctg atgatcgtaa agctagatgt tttgattcct ctggcatgcg
     6661 ctgcatttct tatttcttct tcgttcccta ctaaaatggg ttccacaagt tcttctgctt
     6721 tcaaccgggc agctgcgcct aaaatacgag catcagtagc ttccgggaaa acgattttga
     6781 tcccacggcg aacaatttta aatcttaaac tatcaaacaa ttccacaaga tgacctccag
     6841 acgtttttat atgtattatc atacactatt ccagaaaaaa agaacagttt attttatctg
     6901 tatcagtcaa aaatgctgga aaaattaagt ttttcacata caaagcgaac tgcactataa
     6961 caggaaagcg ctgttttatt acactgtttc aggcagttgc caatcaatcg gttcttctcc
     7021 taacgcaagg agcgcatcat tcgttttcga gaagggtttg gaaccaaaaa agccacgact
     7081 tgcagataac ggactgggat gtggagatgt tatgatgata tggcgactct tatcgatcat
     7141 tttgattttt tcttgtgccg gttttcccca caagataaac acgattggtt tttcccgttc
     7201 atttaatttt tcaatgatcg tgtctgttaa tctttcccaa cctttcccgc gatgagaata
     7261 cgcttgccct tcccgaacgg taagaacggt gttgagtaaa aggactccct gtttagccca
     7321 tgacactaga tttccatgtt gaacaggtgc gattccaaga tcatcataaa gctctttata
     7381 gatatttctt aacgatggcg ggatctttac gccaggttgc acagaaaaag ataagccatg
     7441 agcttgattt tctccatgat aaggatcttg tcctaaaatc accactttaa cttcttcata
     7501 aggagtcaat tccaatgctt catagatatg atacatatct ggatggatct tttgggacgc
     7561 atattctttt tttaaaaatt ctcttagatg cagataataa tcttttgaaa attctgagga
     7621 caaaacttct tgccaactgt tatgaatgat ttctttcaaa aactccacct cctaataaaa
     7681 agaataactc acattttctt caatcacaag ggacaatctt caccctcagc acttgattaa
     7741 tatggtaaac taggaacaag aagcagaaag gaacgataat cattatgatc aaattaattg
     7801 catcagatat ggacggcaca ttattagatg cccatatgag tgtttctcct gaaaatgccg
     7861 aggcgatacg ttttgcaaat gatgccggtg ttgagttcat ggtcgctact ggtcgaaact
     7921 atcaagaagc tcgcgcagct ttagatgagg taggaatcga ttgtgcgatg atcactttga
     7981 acggggcaca ggtatttgat aaagaaggaa attcattgtt taccgtgtcg attccaaata
     8041 atcaagcaat ctctgtttta gatattctag atgctaacgg tatttactat gaagtagcaa
     8101 ccaacgatgg tctttattct gaaaatcaag cgaaacgaat tgaaagtttt gcttccatgg
     8161 tggctaccca tttacctcac cttacgtata aaatggcgat tgccatggcc tcagctaagc
     8221 tggagttatt gcacatcaca tacgtggaca gtattcgtgc tattttggaa gaaaagaatc
     8281 tggaagtttt aaaaattatt tgttttcata cagaagggcc agctgtatta ggtccagttg
     8341 gaaagcagat ttctctttta ggcgatctag ccgttacttc ctctggtcaa aacaatatcg
     8401 aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc ccatgtcgct cacgagcgtg
     8461 ggatttcttt agaagaagtc atgacgattg gcgataactt caatgatgtc agcatgctgc
     8521 aagtagcagg agtcagtttc gcaatgggaa atgcagaaat cgaagtaaaa gattatgcca
     8581 aatatacaac ggacacaaac cttgaatctg gcgtaggaaa agccatattg cgcgcgattg
     8641 cagaagactt gtagttttat ctagaaaaga gggatataga tgtcagaatt tttcattcag
     8701 gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa acgaaaaagg acattccctg
     8761 tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat tgtctcttta tgccatgaat
     8821 ggcgatctcg ttgctcacat caagcagata agcctgacat tcggtacgcg ttttgagctg
     8881 tataaagagt tcgaaaaggt gggaaccatg cagaaaatat tcaattggcc cggtgacttt
     8941 tattatattc gtagacttca ctggacagtc caaggtgata tctataatca tcaatatcaa
     9001 atccatcatt tcaatcagca aatcatgaaa atggataaag caacactttt ttcaggagat
     9061 tactatgtat tggatgtctc aaatgatgaa gatgctccag tttgtatttg tattgctgcg
     9121 gttttagatt actggctcta ccgtaaagac aaaagtcgcg ataagcctgt ttcttaccga
     9181 cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat acgcatttca ggtacatctt
     9241 tttagatatt cttttgcata tgatcataaa gatgaccact gatcatccgc tgaccgacaa
     9301 ctttgaagtc atggcgttcg tataattttt tagcctttgg atttttttca tccacactta
     9361 gaccgatgac ggagcgatta gctttcttcg ccaatttagg taatgcttct aataatcgcg
     9421 aaccgatgcc ttgtcctcta aaatcttctg atacactgat agagtccaaa taccattcgt
     9481 tgggaaacgt ttctttatca atgaacaaac gtgtagacgc atctagctga tatttctcta
     9541 agataggtac cagtggctga tcgatgattg attcttcttc atctgtataa ccgtaagcaa
     9601 ctcctgcaac tttaccgtct atttcttcta cgatacctcg tgaataactg taacgataat
     9661 ttggatcagt gattgcttct tctaaaatct ctaaagtctt ttgttcccct gtctgcttca
     9721 aaaatggcag ttccatatct tttagaatca ctaagatcaa tggggcgatt tctggagcat
     9781 cttcttttct cgcaaaacga atcatagttt cctctccttt tctccattct aacatgcaga
     9841 aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat cttttcagta atgaaataac
     9901 catgagtaac gattcttgcc gcttcagttc ggcaataagg gcagacgact ggcatttctt
     9961 tatcattatg ttcgactaca ttattgactt cttcgtatgc aaattttttt ccgcaattag
    10021 ggcatttcat tttttccatg ttatctcaac ttcctttcat ctttactctc ttagcttact
    10081 cttatattgt aaaaaagaga agcaatatcc ttcttctatg cagatatcca tacctgacgg
    10141 tcctgttctc catctattaa tcggacttta taataaggac gttcatcttt gactagaagt
    10201 gtccactctt tcacttttgc ttttttcccg tagtagctga ctgcttcctt catcgctgct
    10261 tgcggttgtt tgaactcttc cgttttttct atatcaaaaa gatgttctgc ggccttttcc
    10321 gtgtcatttc gtacggttgt tttcccagtg gatggattga ctgctatgac ttgtccttca
    10381 caaaaaaact ggtattcata tgttgcattc gtaggattct ccaaatcttt ttcaactgca
    10441 aaatgaatag cagatatgtt atgttctttt gtttcgtttt ttacgattgc tgctgctttt
    10501 ttcaatgaga tccgatattc ctggtcacta atatcatcca tgtgaaaagc ggcattgagc
    10561 agtcccgtca taccggtaat ccctattacg atgagggcag caacaccaaa catcattttt
    10621 ttcattcttc attctcctgt ttcttttttt taaagaatat caagaaatga acgaatgaaa
    10681 aatcgaatga aacgaaaaaa aagtgacaat tttgttatct ttattcgtgc agttctaatg
    10741 gcaatccgtc tggatcaaag aaaaacgtca ttttttttcc tgtaaaggta tcttctctaa
    10801 tcggttctga ttcgatccct aaggactgca attctgcaat tgtttcttca atatcgtcta
    10861 ctttgaaagc aagatgacgc aagccacaag cttctggata gtttggtcgt tgtggcgcat
    10921 tagcaattcc aaaaatctcc aattcagcac ttcctagttt cagatctaat ttatagtcat
    10981 ctcgttcttt gcggtaattt tctctgatta cttcaaatcc taatttatta acataaaaat
    11041 cacgtgattt ctgataatct gaaacaatga tggctatatg atggatcgtt gtaaggttca
    11101 tatgagcact cctattcttc gtttaagctc ggatctatat taatctccag ttccattgta
    11161 aatgtgtcag attgaaaaaa atagctgttt ttccttttta taagaaaaaa ggcaaaaaaa
    11221 acagaaagta ggtcctccaa gacactcttc ctgttcccaa tataataaat gaaagtaaga
    11281 aattatattt tcaagaaaac aaacagaaaa ttatcataac acaaattatt tataattgaa
    11341 agactagttt tttttcaaga aaagcataca atagattgtg ttagtaaata cagaacacac
    11401 tacatttagt gtcttaagga gtcctcatac atgaagtcat attttcagtt tttagctcat
    11461 cagctaaaag gctggccgca acaaaattat tatcttttct ttttcagtct tggctgccag
    11521 gtcatgactc ttgtcaatag tcccatcact tggctgtccg ttattacatt tatcggtacc
    11581 acgcttggtg tattgtgtat tttatccatc aatgcggcaa aatcagtcaa cggtatttta
    11641 ggtattttgt ctgcgatttg ttttattatc gttgggtttt cagcaaagaa ctatttaagt
    11701 atcggtgaac agattgctta tatgatcaca ttggatatcc ccgtattgtt aagtaaagaa
    11761 tggaatcata atatggcttc aaaaatccga aaattcactg gaaaaagctg gtttatcgca
    11821 atcatcagta ctttccttgt ctatcttgtt tcaggattcc tgattggtcg ttatacagat
    11881 gatcctcggc cgtgggtaga tgccattgct ttttctatta gcttgactgc tggtatcatc
    11941 agtttcttgc gttacaacaa ccaatattac tggtggttag cttcagggct tgctcaaatg
    12001 gtcctttggt atatttcatt tacgcatgga tctgcaagtt tggcgatgtt cgtgaacagc
    12061 tctgtttact tgctgaatga tgtccttgca tttaccgttt caccttggta taaccaaaaa
    12121 gaacgtgaac gtatgcaaaa aatggaagaa gaatactaca acgcacaaca acacgcataa
    12181 aaaaactgag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata
    12241 aacggcggaa tcagaagcaa ctccttcgga aataagctga aattcacaaa aatttgaaaa
    12301 gcaattttcg tgaatttctt cttatttctt ggagttaaac gcttctgtcc cagcctctat
    12361 tttttatagt aattccttca gatactttct tgcctcatca ttgaaacgca gtgattcttc
    12421 taacttctga taatattcca acgcttgttt ctgagcttca gtaaaacgat aatgttttcc
    12481 tttccatttg tcgattgtct cttgcacatt tccagattgg atatctttca ctactagttg
    12541 ccatttttct tttgagactg tacggtcttt cttatcagca gattgtatag cttctcgttc
    12601 tttacgcaat acttttaaaa aaccatttct gactatccga ggaatgctgt cgaagaaccc
    12661 ttctgtaaaa ccatagcgac aatattgctt ccaaataaat agatagattt ttggttggat
    12721 gaatcgttgg tttttcgcaa aatattccat ctgagcagtg acttctggtg ttcgttgacg
    12781 ttttgtcttt ctcaacacgg aaataaatgt ttcaaaataa gcattaggat gtactggcac
    12841 ttcttcatat acgatggatt gccaatcttt acgtaaaaat gcgcgatagt tgatcaagaa
    12901 ttcttctaca tcctttacta attcttctgc aaagtcgtca aatagttctt tttttatctg
    12961 tcgctttttt gactgcccat tgatgtgttc acttattttc ttgatttcat tttccaatgc
    13021 ttgataatcc gctatgtaaa tcggcatttc tacttggcga aattctgcga gccgctgagc
    13081 taattttata tccggtaaat atggctgttt catccgacga ttttggtaca agacaaaaca
    13141 gtatttttgc catagtcctt caggaaaatc gatattcttc tgcttaagcc ataataattc
    13201 gtctaaggcc agatcaactt ttgtcattcc ctgatggatc gaagctgcca ctgtcggaga
    13261 gacatgcagg tgatcggcaa agtggttgat gcctagtttt aggatctttc cggtttttgg
    13321 cgatttaacg atgtattgca cttttaattc tctaccacac tcacaataca atttttgctg
    13381 gcttcgtttt gttcgaaaaa acggattgat cttgaagtca gaaaacaccc aatctgttgc
    13441 agctaaataa tgttcttcca taaaacgaga gctgattaga tatcggcggt gcttatcgac
    13501 tagttcccgc tgttttttcg ttaattttgc atagatctcc caacgttgac tgcttgtgta
    13561 ttgtttagaa attttgatgg gatagctttt gctgatttct tttggaaacc attcttcaaa
    13621 gacggattgc cattcttcca cggctgtttc tttcctttct aattacaaaa agctggctac
    13681 taacataacg ttagcaacca gccagtgttt cttgctaaat tagtgagttg ttccggcatt
    13741 tccggaaata tcttctttat tttctactgc tgcttcaact gcataagtca aagaagaagc
    13801 aatgtcttct aaagacatag aaggcgtacc cactggacga tcgatcactt gatcgggtga
    13861 gaatggcacg tggatgaacc cacctcgaat tgccgggaat tgtgtttcga tcaaatgaag
    13921 caaacgatac atgatatcat tgcagacaaa tgttcctgct gtataagaga tgtacgctgg
    13981 tagaccatga tcttgtacat tttttgtcat tgctttgatt ggcaatgagg taaagtaagc
    14041 ggctggaccg ttttcttcta aagccgtatc gaatggctga tttccttcat tatcagggat
    14101 tcttgcttct gctagattga ttgcgacacg ttcgaacgag actgctgacc gcccacctgc
    14161 ttgtccgaca cagatcacga catctggttg gtgctgttgg atggcttctt tgactacttg
    14221 actgcttttt tcgaatacgg tcggtacttc cactttgatg atttcagcac cgctgatttc
    14281 atctggcatt ttttttactg cttcataggc tggattcact ttgtccccac caaatggatc
    14341 aaaacctgtt actagtactt tcatctgttg ttcctccgtc cgattgattt aacatatggt
    14401 tatcttttat cataccattt
//
LOCUS       DACDMM010000063        14165 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDMM010000063 DACDMM010000000
VERSION     DACDMM010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.063"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..428)
                     /locus_tag="IWD16_001482"
     CDS             complement(<1..428)
                     /locus_tag="IWD16_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9088545.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHAT"
     gene            complement(676..1512)
                     /locus_tag="IWD16_001483"
     CDS             complement(676..1512)
                     /locus_tag="IWD16_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP9088546.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1523..2452)
                     /locus_tag="IWD16_001484"
     CDS             complement(1523..2452)
                     /locus_tag="IWD16_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP9088547.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2644..2997
                     /locus_tag="IWD16_001485"
     CDS             2644..2997
                     /locus_tag="IWD16_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9088548.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3071..3727)
                     /locus_tag="IWD16_001486"
     CDS             complement(3071..3727)
                     /locus_tag="IWD16_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9088549.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3953..5887
                     /locus_tag="IWD16_001487"
     CDS             3953..5887
                     /locus_tag="IWD16_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9088550.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5973..7196)
                     /locus_tag="IWD16_001488"
     CDS             complement(5973..7196)
                     /locus_tag="IWD16_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP9088551.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7190..9088)
                     /locus_tag="IWD16_001489"
     CDS             complement(7190..9088)
                     /locus_tag="IWD16_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP9088552.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9082..10200)
                     /locus_tag="IWD16_001490"
     CDS             complement(9082..10200)
                     /locus_tag="IWD16_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9088553.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10283..11116)
                     /locus_tag="IWD16_001491"
     CDS             complement(10283..11116)
                     /locus_tag="IWD16_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9088554.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11109..12026)
                     /locus_tag="IWD16_001492"
     CDS             complement(11109..12026)
                     /locus_tag="IWD16_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9088555.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12253..13377)
                     /locus_tag="IWD16_001493"
     CDS             complement(12253..13377)
                     /locus_tag="IWD16_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9088556.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13560..>14165
                     /locus_tag="IWD16_001494"
     CDS             13560..>14165
                     /locus_tag="IWD16_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9088557.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVD"
BASE COUNT         4461 a         2815 c         2291 g         4598 t
ORIGIN      
        1 ttcgtagcat gttgaacagg atagttttct ttttctttca ataagtaact ttggatgctt
       61 tttttatttt tttcataagt gaattctttt atcaaataac cactaggcgt aatctttcga
      121 ttaataacag ttattgcttt attcggatga gacgttagat aaacaatttt acacttttca
      181 ttcttttctc taagcatttc gcctagttcg ataccgctat aataggcgtt caaatcgatg
      241 tctaggaaaa aaatatctga ggaatagatt tcattatttt taatgaattc aacaaattgt
      301 ataacgttca atccctgaac gatctcaaat ttccaaggac ttaataaaat tctggattct
      361 tccaaaattt ctactagcat tttttcaaaa accaaactat catccaaaat aaatatacgc
      421 gttaccataa aaagtacctc cactcgatat tttactatca aaatcatttc atacaacttt
      481 tcaacataca tgttaggaat aaattgtaat tctcttcaca atcttttcca acacctttga
      541 aaccgatatc aaatactttc aggatgctgc ttctaaaata aatatcaaac aaatgaatac
      601 atcatgcaaa aaaagccaga caaccgacgt ccttgcgtgt cagctgtctg agctatgttt
      661 atttattttg ttcttttaaa aagaacgata cacatactga tttttcggag cagagacttt
      721 ctcagcgttt gtaacaacta cagatgggat ttcacgcttg aatggttgat aatagtctga
      781 ttggatggtt ccttctattt tataccattc atcattttta accgttactc cttccggcat
      841 atgcgtcaac aaaccaaaga ctcctgaatc tgccacacaa tggataatgc cgaatcggaa
      901 caagaagata tctgtattgc tatcgttcgg tgtttggtaa acaaatcctt catagctgat
      961 ttttttgcca atgaattcgc tagggtaatt ataaattagc tccataactt ctaaatagtt
     1021 ttcatctgtg atcttgatag tttgcttatc cccatacttt tctttcaact cattcatttg
     1081 gtctaagtag tctgatttgt tgaaataaat actggtatcc ggctttaagt attgtgtctg
     1141 catctccgga tcccctactg attctttact tactgggaaa ttaaatcctt tggcttctac
     1201 gatcgtagtg tcgagactaa ctgttggaaa caacgtcccc acgattactg gcaaagctag
     1261 cagtacataa gcgattccac gttgataagg ttttgacaac ccatggtcat gatcgtgctc
     1321 gtgtgccttt tcttctgctt ttccgttttt gacccacaaa tacagctgaa ctaaagccaa
     1381 aagagcagat aaaatcattg aaagaatagc taagtagcga taatggacat tgatatattg
     1441 atcgagtttg ccagaaactt gcaagtacat catcaattcg aaatacccag ataaaatcaa
     1501 aaaccgcagc atattttccc tcctaaataa ataatgtgaa tccaataacg ctaagggcaa
     1561 tcagtccgat cagcgataaa ataaagcgtc ctgtaaaata tcgtttcatc atcagtagat
     1621 ttttgatatc gaccattggg ccaaagacca aaaaggcagc tacaggtgct gtgccgaaca
     1681 gacttaacaa agaagatcct ataaacgcat ctgcttctga gcaaagagac atcgtaaagg
     1741 ccaataacag catgatgata atggctaaaa tctttgttga gccgagcgtt agcataattc
     1801 tagttggtaa ataagtctgc atcgcagctg cgattaatcc gccaatgatc aaataacgac
     1861 ctgtatcaaa aaattcgtcg attgaatgtg tcaacacatg cccagtttga tggatcagct
     1921 gattttttgg ttcattcttc tcactttgtt cttcttcatg gacatgatga tggtgatttt
     1981 ctgctacttt agacgtgatt ttttctttca atacagcgtc tttttgaaaa taggcaagcc
     2041 agacaccaat cacaagggca acaaaaaagc tgcctatcac ccgccagctt gccatctgcc
     2101 aagaattgcc aaatgcgata aaagtggaaa aaatcacgat tggattgatg atcggagcag
     2161 tcagcataaa agcaaacgct gtgtgaactg gtacgccttt tttaacaaat tgatgaacga
     2221 tcggtacgat gccgcattca catgaaggaa agaaaaatcc taagatcgaa ccagtaaaaa
     2281 tagagaagat acgattttca ggaagccatc tcttcacacg ttctggggtc aaaaatactt
     2341 gtaaggcgcc tgagataaga cagcctaata atacgaaagg taatgcttca ataacgatcg
     2401 ataagaaaat cgtactcatc tgcaaaaccg aatttggaat aaaagaaaac atgagtttcc
     2461 tcctttttat ttctagttca ttcgctttgc aaatatacgc ttttattcaa aaaaaacaag
     2521 ttgctctgta aatttttaca caactcttat tttttaccaa agcacttcaa ttgacttttt
     2581 ctgttattcc cattaaatta aggtctcata catatcactt ctatcataaa agggagaata
     2641 aaaatgaaac aaaccactaa cttgactgaa gttcaagaca tactgcaaca gtcatccgtt
     2701 gccgtcattt atctttcgat gcctaattgc agtgtttgtc acgctgttcg tccacgacta
     2761 gaggaacttc tcacacatta tgatattccg gcacttcatt tggatgcatt tgaaacacca
     2821 gaagtagcta gtaaatttga agtgctgact gcgccagttg tcctgatttt tcatcaagga
     2881 aaagaagtct ttcggcaagc acggttcatc gactttaaaa aaatccgtta tctactagat
     2941 gaactgacag cagaagatga ttccctttct tatgaagaaa tcttccgaac aaaataatcg
     3001 ttgtccaaac agaaaaaagc gattgaacaa gtgaaagttt tgccttcatt tgttcaatcg
     3061 ctttttattt ttattctgct gtattttcgg aatgcgcatt tgcgtcagaa tgcaagaaat
     3121 agatcaaggt ttgcaattca ttcgtcaaat caacattttg gatcaataca tctgacggag
     3181 cattcaatcg agcagcggta aagttcaaga ttcctttgat tcctgcttcc gttaactgat
     3241 tgactacttc ttgtgctctt tcagcgggta tcgttaaaat tgctacttcg atttgttgga
     3301 tcttgatttg ttctgacata tcttccattg gataaacggg gataccgtct acgatgcgcc
     3361 caacgatttc ctcattcaca tcaaaagcac aactgatacg tatgctatta ctttgatgga
     3421 atttgtattt tagtaaagca cttcccaagt tccctacacc gatcagcgca acatttgtca
     3481 attgatcgtc gtttaatgtc tttgcaaaaa agtgcataag atcttctaca tcataaccat
     3541 acccacgttt tcctaattcg ccaaaataag aaaaatctcg acggatcgtt gcactatcta
     3601 cttgaacggc ttcacttaat tcagttgaag acactttttt ctttccagca ttatttaaaa
     3661 tccttaaata gcgataatac aaaggcagtc ttttagctgt cgcctttgga ataatatgat
     3721 ctttcacgaa atgacctcca cgtattgtct aattacatct ttgatttctc catcatagca
     3781 gtatttatag gaaaaaatca attccattgc tcagatattg tgaaatttta ttctttattc
     3841 atttatttat catttctgca aatctcgcag tataatggaa tttatggtag actactaatt
     3901 gaaaaatagt tttttatttg aggagaattc acaaagtaga aaagaggatt ccatgatatt
     3961 attacaagcc aatcagattg ctcgccattt tggtgcagaa gtcttatttg aaaacataca
     4021 tttagaaatc agtacaggcg ctcgtgtcgc tttagttggc agaaatggcg ctggaaaatc
     4081 gactttatta aagatcattg ctggaattga agcacctgat gaaggaaaga tcgctaaaaa
     4141 taaaacagcg actctcggat acctcgctca ggacacagga cttgattcca atgaaacagt
     4201 ttggaacgag atgctgaaag cttttgaaca agttagacaa atggaagagc ggatgcgcga
     4261 gctagagatt ttcattagcg aaggtacgcc agacacacct gcttatagtt cttatttaaa
     4321 agaatacgac cggctgcaac acgatttttc agatcgaaac ggctatggtt atgaaaatga
     4381 aattcgttcc gtattgcatg ggtttaaatt cgattcttca ttttatgatc agaaaatcga
     4441 tactttatca ggcggacagc gcacacgttt agctttagct cgcatgcttt tgcaacaccc
     4501 tgacattttg attctagatg aaccaacgaa ccatttagat atcgagacac tttcctggct
     4561 tgagaattat ctgcaaagct atactggtgc attattgatt gtttcccatg accgttattt
     4621 tttagataaa gtagtcaatg aagtctatga attaagcaga agaaaaatgc gccattacaa
     4681 aggtaactat tcgaaatatc tagatttgaa agcagaacag ttagcaagtg aatggaaggc
     4741 ttatgaaaag cagcaaacag aaatccataa attagaagac ttcgttgctc ggaaccttgt
     4801 tcgcgcctct accacaaaac gagcgcaaag ccgcagaaag acattagaaa aaatggaccg
     4861 gctggatcgt cctcaagggg atgaaaaaca agcaaacttt ctattcagta tagaaaagcc
     4921 ttctggaaat gtagtcttgc aaatcgaaga tgccgctatc ggatatgata atgtcacctt
     4981 atctgaacca atcaatctag atatccgtcg tcaagaagcg attgctctag taggtccgaa
     5041 tggtatcggg aaatcgacgt tgctgaaatc aatcattcac cagatccctt ttatccgtgg
     5101 aaaagaaacg ctagggacaa atgtcaccat cggctattac gatcaaggtc aagctgatct
     5161 gcatacaaac aagacgatcc tagcagaatt gtgggatgag catcctacca ctccagaaaa
     5221 agagatccga agtgctttag gtgctttctt attttctggc gaagatgtag aaaaaccaat
     5281 ccctctgcta agtggcggtg aaaaagcacg ggttgcttta gccaagttag cgatgaacaa
     5341 agaaaatttt ttgattttgg atgaaccaac aaaccattta gatattgaca acaaagaagt
     5401 attagaaaat gcattgatcg attatgacgg cacactgctt tttgtttccc atgaccgata
     5461 tttcatcaat cggatcgcga caaaagtcgt cgaactctct gaaaatggca gcaaagtcta
     5521 tctaggagac tatgattatt atttagagaa aaagcaggaa gaagaagaaa tcgccgaatt
     5581 gttggcacaa gatgagccag aatctgctcc tgctcccacg aacaaatctg attattacca
     5641 aagcaaagaa cagcaaaagc ttatccgtag ccttcaacga aaaattactc aggttgaaga
     5701 agaaatggcg cggatcgatg aatggatcga tcagttggaa caagaaatgg tcgcgccaga
     5761 aaacctggaa gatcacgtaa aactcaatga gctgaaccaa gatttagaag ctgctagaca
     5821 agatcaagaa acaaaattga cggaatggga agaactaagc ttggaattag aagaatatga
     5881 aaattaacga aaaaaccaaa acagaaacca ttcatgctgt tttggttttt ctttttttat
     5941 tttatcaaac aaaaattcca tatttacctt tttcaccaat aagaagacca attactagtc
     6001 attctcacta aagcttctaa ataatagtaa tcaccccaaa tacaaggtgt atttacccca
     6061 ttatttttgg gcttattata aacagactgt gttaaaaagc catcttcttc ttccgttgta
     6121 gcatatcttt ctttcaattg tcgcataatt ttccaaacag cagactggta taagttcttt
     6181 tctgttttgt ctaatagacc tgattctatt aattctaata atccacaaac catgatcacc
     6241 atactagatg tatctttttc ttctctgcta gaatcatcaa agatcaaatc ccaataacaa
     6301 atggtatctt ctggcaaccg attcataaaa taattggcta atattttgct taaatcaaaa
     6361 tatttttgtt ctttcaaata acgataactt aatgcaaaac cgtaaatccc ccatgcttgg
     6421 cctctagccc aacaggaatt atcactataa ccttgatgcg tacttccata tcttggtttt
     6481 ccatctttga catccatata aaatgtatga taagttgaac catcttctct tactaaataa
     6541 tctgctgcat tttgaatatg agcctgagca gcttttttat atttttgatt tccagtttct
     6601 tcactcgccc aatacaataa tggaagattc atgcaacaat ccatgatcat tctaccttgt
     6661 tgtgaaggat cactaggatc tccccaagct tgtataactc ctgccttctc ccaaaaacgc
     6721 tgaagtaatg tttctgcagc atcaattgaa gcttccttca tttttcgatt tcccgttatt
     6781 ttataatctg ctacggttga taaagaatac aaaaaaccaa gatcgtgatg attagtgata
     6841 atttgacagt caatcctgtg gataaaatct atatctgctt tttcagctgt ttgttgaaat
     6901 aaaggatcac ccgtcttctc aaataacaac caattaattc ctgtccaaaa tccttctgtc
     6961 caaccaatat tgtctcttaa tggataacgt ccttttatag gacactcgct tgggaattga
     7021 tactgactgt tactgatacg tttgattaac ctctccgtgt gtctgcgaca acgctcgatc
     7081 tcattattcc attctttttc cattaattct ttataacatt catttatcca tttttctgga
     7141 tgtattatct cttcaaacat cactttatca agcatttccc caccagcctt tagcataatt
     7201 ttagtctttt tttattgttt ttattttgga tagttagccc ggttgtccca tcaaaatcaa
     7261 ttttgggacg attgttccat acttctaaac tataatcccg atcttgatga gcaaccactc
     7321 ctgttccgag ccaatgggtt ccttttccaa atgtaccctc taatccagga ataaaagtgt
     7381 tttcccatgt catcaagttt gtattgggca aacccataat agctgtggat tttcgtttaa
     7441 tagtatcgtc acttaaacag ataatacctg aaaagccagc ttcactcacc ctatagtttt
     7501 ccttgtcttc ttctatgatt attccttctt tctcaggaga acattcaata gcaaaccctg
     7561 tctcatatag aacatattct gtatctaatt ctactttgtg tacgcgaatc tgccagcctt
     7621 caaaaggaat aagccaagtt ctgatttttg cctgtaaagt aggttcccat gtagaggcaa
     7681 gtacctcttt cttacataca atgtctcgta cattatttcg aacgatgaac tgtccatagg
     7741 ttttgatgct ctcattctta aaactggtcg tgatatatgt gtccgctcgc tgaatcccta
     7801 gcattgaatc cattgccatt tctgctttca attgattcgc tcgcggtatc gaaaaaccaa
     7861 attgactaga ataagcaaat tttaaatatt tttcattggt caatgtatgg aaatttggcc
     7921 ctaattgtcc cgcattcaaa aaaaatgtat gtccattatc atgttgtatg agcaaattgg
     7981 cttctgaaag aagtttagtt ccttcacgaa taggcatagg catttcttta gccgtccaat
     8041 actcatggtc ttcaggtagc gccaacaata aaaaaatctt attcagccat aatggagata
     8101 acgtactatt atagggttca gtcaatgtca attgagggta ggtataacca atattcagga
     8161 tacctttttc atcaaatatt tctttttgca tccaccatct tatattccga ttgactaacc
     8221 ccttgatttc acctaattta aaaggcaaaa gatcggccca caacatagct gaccaaaagc
     8281 ttgaaactgc aaaacgataa atcatgcttc gaccaaaagg cacatttgca ccatcttccg
     8341 caaaaaaatg aatatagtct ctagaaaaat caatagctcg gttcttatat atctggctaa
     8401 tgggcttttc tggttctagt acgctataaa tcaaaccata ataatgaaat gcaaaaggat
     8461 tataataatc ttggcgacct cttgaactgt ctcgatacca gccattttga agataacaat
     8521 tttctatatt atcaaatgct tctttcagtg ctgattcttt aactggataa ccaaattttt
     8581 ggataattgt tccgacaatt atcttaaaaa attgccaatt accatctgca cattcatatt
     8641 caaaaatttg cgagaaccaa tgaataatct ggcttttttc tcctgtatct aattttttcc
     8701 ataataattg ttcatgatga tataacgcta ataacattgc gggcatttct accatacgct
     8761 ggtctctttc atgtatttct ccccaataat cgggatggga aggatctgtt ccagctttta
     8821 ttcctgatag aattaatttc aaccatcgtt catctaattg gtcaattgcg ggccccgctc
     8881 cccataaaac ccgagagaat ccttccattc ctgcaatttt atttccatat cctacccctt
     8941 gatgtatata atcaatttgt gcgccacttg ctcgataaaa cggctggagt ggttgattta
     9001 tctttttaaa taagtcaaag taatcctgtc tggtccttaa tagtggtact ggatgtttca
     9061 ttgtattttc tcctcttttt actaccaaat agctaattcc gactgttcta atcgaagtaa
     9121 gccttcaata aaaaaataat cagaatagat cagtggcaca ttataagtca gttctctatg
     9181 gtatgcttct gctccgcctc caatgatccc atcttcatcc atattccaat ttgcatagtg
     9241 cttttctgtc ttttggagta tattgatagc agcttcatga tacatttttg atcgtacaga
     9301 ggaaacatat cctgctaatt ccagaagacc gcatgcagcg catactccag cactggtatc
     9361 aatattcgaa tcttttacag caaaatcaat gagaggaata taaccagttt tttcgatttt
     9421 ctgaataaaa tttactgcta tcttttctgc tatagctaga tagtacggat tttttgtgta
     9481 acgataactt aacgcgaatc catataaagc ccatgcattt cctctgctcc aacaagaatt
     9541 ctcattgtat ccttgcccgc caagtaaccg aatatatttc cctgattctg catcaaattc
     9601 tgcgatatgc ccggttgatc catctttcct cactagatac ttagctgttg tatctgcatg
     9661 ctgccgagca attttagaga acctcggatc accagtttcc ctcgaagccc aatacagtaa
     9721 tggtaaattc atcatactat caataatcat ccaaccaggt ttatggtcat tccatgctac
     9781 taaatagttc cctgaaatat tatagcgtcc agcaagtacc gtagctgcat gtaaccctag
     9841 ttgtcgacta tgtttgtttt gagactgttg ataatgtgca atagccgatg gtaagaataa
     9901 aaaacctaca tcatgatgaa cgtctgaaaa atggtacagg gcagattcta ataattgttc
     9961 ttgttgtatt gctgcctctt tgaacttttc tcgttttgtc ttgtgaaaga gctgccataa
    10021 aataccactc caaaaaccat tggtccacca gtcgattcct ctgtccttta ttacgtctgt
    10081 atattttccg ttgtaagaaa tatatggaat tttattcccc attctgtcta attccgcatc
    10141 tatcttttca gaagctacat tcaaaatttt tcttgaccat tccttcgtct gttcattcat
    10201 ggatatcccg ccattctttt aatttttatg aatgtggagt tgctcctaga acgaactaga
    10261 tggttaacat tcgttatttt ttttacttat ttgcagctcc aatgtcattc tccttaaatt
    10321 tagactgcgc ccacacataa atcaagacca ttgggatcac tgaagttgct aaaattagca
    10381 taattgcgcc ataattaatt ggctcacctg tactagaaac cgtcatattt ttgataatta
    10441 agggaaccgt atatttatct ttatctgaaa gcatgactaa tggaaataga tattgattcc
    10501 aacttcccat aaagataagt aaaccactag taataatcgt aggtgataaa atcggtacag
    10561 cgattttcgt aaaaatcacc cactcattgg caccatcgat tctggcagcc tccatcagtg
    10621 ctgtaggaaa ttgactgcta tattgtcgca tcataaacac tgaagttgta ctggcaatcg
    10681 aagggatgat cacacctgca taagtattcg ataatcctaa acgactcata atgtaaaata
    10741 aaggaatcaa tgtggtaaaa aaaggcacca tcctagatag cataactgta ttgaacaaca
    10801 gtttattccc cataaaacga tactttgcta atgcataacc cgccattgta gaactggctg
    10861 tagctaaaac tgtactgatt acagcaataa caatcgaatt ccaaataatc aagggataat
    10921 tatatctcgc catgatggct tgccagtttt ttaatagcat atccccaaac ttgaaattga
    10981 ttccgccact taaaatttct tgattacttt gcgttgaagc tacaatcata tataaatatg
    11041 gcatcaaaaa tattaaggca ccaaacacaa taatgacgta cgtaaaaatt ctgctttgta
    11101 tcttttgttt agtcattttt atcacctccg aatttaaatt gaatagccga taatataaca
    11161 gatacaaaca ccatcgtcca ggcaatagca gaggcttttc caaaatcaac catttcaaaa
    11221 cttgttttat ataaattgac tcctacgacc aaagctgaat tttctggtcc attggaagtc
    11281 ccaaagaata tatttgggat attgaaatta cccaagccat tgatagtggc taaaactgtg
    11341 gccattaaaa tgattggctt tagcatgggg atcgttaata aaaagaattg ccgaaatttc
    11401 gatgctccat ctatttcagc actttcgtat atctctttag aaatcccttg aagaccagct
    11461 aaaaataaaa tagtgttata tcctacacta agccaaatca tcgtgatgat cattgatatt
    11521 cgtatccaaa aaggatttgt taaccactgt acaccgttaa ttcctagcgc attcaaaaat
    11581 ccattgaata acccagtcgg attgaaaaaa gtcatgaata cggaagctac agccaccgtt
    11641 gatgtaatgg ttggaatata atagacagtt ctaaaaaatc cttttccttt taactgtgct
    11701 gaattcaata aaacagcaaa gtaaatagct aatagcatcg ttattggaat tgatcctgcc
    11761 atgataatca aaacattcgt gtaagattta ataaacaatg gatctcggaa gatagctaga
    11821 taattgtcaa atccaacaaa ttcaaatgag tagccaattt tcataaaact aaaagcaaac
    11881 gagataatga tgggaatcaa aatcgtcaca ataaagaaaa taacaaaagg tgtgaccaat
    11941 ccataagcac tttttttatg agacgtatct gttgcttttt tcttgctgtt ctttattgtc
    12001 ttgcttggta acgaagattg catcatcgtt aagtcctcct aatatttttt aatcaccagt
    12061 taattttacg ccgtacttat gagcaaaatt ttctgcttct tttagatatg aattttcaaa
    12121 atcacctgtt tgccaatagt tatatgcagc ggtagtcaaa taagacgtag catctgaata
    12181 agtggctggt aaaacagtat ttgatgtatt ttcattgatt ttattcaata gttcatagta
    12241 tttttgtcct ccttagtagt caaacttttt ctcagctgct tctgtttgat aggccaatga
    12301 gttagatgta gccacgccca gctccagtgc gttggctaag cattcttcat ctgtcagcat
    12361 aaatgtaaga aattgttgtg ccacttgtgg atttttggca tctttaggga cataaaaact
    12421 tgatcctcct gatactgcac cttttcctgg attttctttt gaaaaaggaa tcagtggagc
    12481 catgcgccat ttgtcagatg catcaggaaa attcaaagac atattcgtag cataccagcc
    12541 gccagcaaca aacattgcag attcttgaaa tgctttctca gcatcttgtg caccgtagaa
    12601 atgtacaata tctgagtcga tcatttcttt gattaacttt gcagcatgtt tcgcttcttt
    12661 agtattcaga cggatatttc cttcttcatc aagcaaagat tgattctgct gggctagcat
    12721 gttgataaac aattcttggt ccccagattc tggcaaggca attccaaaac taccagtttt
    12781 ttcttttaat gcttttgcca cctggatata ttcttcccaa gaactgattt cttcaaaatc
    12841 tacacctatt tcagcaaatt tttttggttg ataataacaa agtgctgggg taaaatcact
    12901 agcccaagga ataatcgttt cattactcgt ttgttgacgc aaaatattca atttttgatc
    12961 ataaaactga tcgccatatt tgtcataaaa tccatattca tcagcactat aaaaagcatc
    13021 ttgaaatttt tccagcacac ctgctgctga agtgtctgcc atggagataa tatccggcat
    13081 atcttcgtgt gttaccaatt ttggggtgat catttgtact gttgctgttc ccgaagctac
    13141 attcacaatt tttaagtcaa tatttgctcc agttttgcgg ttatagattt ttgcagcatt
    13201 ttcaacatag tttgtcagtt tgccgtttgc gacatatact tctaatgtag tggctttttt
    13261 tatcattgga tctgcgatgt ttgctgttac ttcttcgtta gtttttccac atgcacttaa
    13321 gctgaataat agggtaatgt ttagcaaagc tatcaccaat ttgtatcttt ttttcatgtc
    13381 tatcccttct ttcttttcaa gttcttatat aaaacgcttt cacttatgta tattaacgga
    13441 attagtaaca caaatattta tataaaaaaa tattttactt gtcgttttgg cttttcattt
    13501 ttgttataat agtcattaat tataaataga aagcttttta tataaaaaga gaaaggaaag
    13561 tggttctttt gttaacaaac aaccaatcct ccaatcttgt caaaaaaaca ctaacctatt
    13621 ccatagatac ccatcttcat ttatctgctg ctccctattt tcgactacta ataaatgaaa
    13681 gcgctaattt atatgctgaa tttgcacata aatctataga actagataat ccttcccttt
    13741 tattcattga taaacagcaa gaagtccaaa tcttttctac tcaaaaggaa acacaaactt
    13801 ttatcattat ccagttttat tttgatgagc aattattagc acatattacg ggacaatctt
    13861 tttctataga tcaagaaata acatctatca ttacttttct gaaaaatgaa cagttcaaaa
    13921 ttgattatct aaaacatagt aagccagcta tttcctatga tcaggaaatc aattcttttt
    13981 tcatactatg ccaacagatg agcattcatt tgctttcctt attagttact tattgtctgc
    14041 aaaaaattca attgtcttcc ttaccaaggc attatcaaca ggaacttgct ttattgacaa
    14101 taccttcaaa tgagaaagtc attcctaaaa aaaagcatat tctttattca gatgaattag
    14161 ttgat
//
LOCUS       DACDMM010000064        13934 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDMM010000064 DACDMM010000000
VERSION     DACDMM010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.064"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(58..603)
                     /locus_tag="IWD16_001495"
     CDS             complement(58..603)
                     /locus_tag="IWD16_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9088558.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(615..1307)
                     /locus_tag="IWD16_001496"
     CDS             complement(615..1307)
                     /locus_tag="IWD16_001496"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP9088559.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1326..1610)
                     /locus_tag="IWD16_001497"
     CDS             complement(1326..1610)
                     /locus_tag="IWD16_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088560.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1623..2186)
                     /locus_tag="IWD16_001498"
     CDS             complement(1623..2186)
                     /locus_tag="IWD16_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9088561.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2414..3076
                     /locus_tag="IWD16_001499"
     CDS             2414..3076
                     /locus_tag="IWD16_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP9088562.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3200..4396
                     /locus_tag="IWD16_001500"
     CDS             3200..4396
                     /locus_tag="IWD16_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP9088563.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4497..5873)
                     /locus_tag="IWD16_001501"
     CDS             complement(4497..5873)
                     /locus_tag="IWD16_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9088564.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5910..6776)
                     /locus_tag="IWD16_001502"
     CDS             complement(5910..6776)
                     /locus_tag="IWD16_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP9088565.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6865..7890)
                     /locus_tag="IWD16_001503"
     CDS             complement(6865..7890)
                     /locus_tag="IWD16_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9088566.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7908..8582)
                     /locus_tag="IWD16_001504"
     CDS             complement(7908..8582)
                     /locus_tag="IWD16_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9088567.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8766..9659)
                     /locus_tag="IWD16_001505"
     CDS             complement(8766..9659)
                     /locus_tag="IWD16_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9088568.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9733..11430)
                     /locus_tag="IWD16_001506"
     CDS             complement(9733..11430)
                     /locus_tag="IWD16_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9088569.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            11552..12484
                     /locus_tag="IWD16_001507"
     CDS             11552..12484
                     /locus_tag="IWD16_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9088570.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTIIEHLWKYF"
BASE COUNT         4291 a         2861 c         2452 g         4330 t
ORIGIN      
        1 tttatgctct tcttttatta ttttgtgctt tttattttat caaatcactt tgttttttta
       61 taaaatttct gcgcctagtg tggactcaaa atgtctcaat gcccattcat gtccaacggg
      121 atcgaaactt gccacggcat ctttgaaaat atggagtttg tatcctagat tatacgcatc
      181 gactgctgta tgcaacacac agatatctgt acatacgccg gtcaaataaa tatctgtgat
      241 ctggcgttct cgcaaacgga tgtctagatc tgttccacta aaagcggaat aatgccgttt
      301 gtctatccaa taaacatttt cctcttgctc atagtcttgg tataaacttt ttaaagagcc
      361 gtacaattcc cgtccacccg ttcctatcac attatgcgga ggaaataagc ggttctccgg
      421 atgaaaggaa tcttttggat catgcgcatc aatggcaaaa acgacaaaat ccttttgatc
      481 aataaacttc tttgtatgag cagtcagctt tttttcaatt acttgtcctg ctgtcccagt
      541 agtcagcttt ccatcctctg caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt
      601 catcttagtc ctccttatac gagatgtttg acaaattcga tcaccatttc tgctgatcgt
      661 ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg
      721 ctcattgcac gtacgatcaa aaatgggata ttgaactgac gagctgtttg tccaacagcg
      781 gctccttcca tttcgcaagc taatgcttca gggaaattcg ttaagatttc tttgatttta
      841 tctggcgagt cgacaaatgt atcacctgta acgatcaatc cttcttttgc gttcaaattc
      901 gttgcttttg ctgctttaac catttcactg cgcaaatatg tgcttgcttc ataatataga
      961 ggcattcctg gtaattgtcc tggtttgtag ccaaatcctg ttgcatctgc atcaaaataa
     1021 gctactttgt ctgatataac gatgtcacct acttgtaagc cttcaccgat tccgccagct
     1081 gaacctgtat tgatcaccat gttcacgccg tattgttgga tcaacagact tgtggtaata
     1141 gatgctaaga cttttcctat accggaacgt acgacgatca cttcgtggtt acctaatgaa
     1201 cctgaaataa ataaagcgcc tgcgcgttcc catgataatg gttcgcttag gttctctctc
     1261 aaaattttta cttcttcttc cattgcgccg ataataccga ttttcatctt tttaattcct
     1321 tctttctata taaatgcgat tgctaaaaaa accacacata acattaggat aacaataata
     1381 agagatttca tcaaaaaaga gttccatttg gtcgtctttt cccgttcgct gatccttgtt
     1441 ttcgtgattg gtttattctt tttgctttct ttacgataaa agctggcaat ctttttttct
     1501 tcttgctgat aagctgtttc tgcttttcgc tgttttttta atgactcgct tgcttgtgct
     1561 tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc ctttggccat aagccgatcc
     1621 ctctactttc ctttggtcaa tagttcccag tattgaatcg cataaatcgt ctttgcatca
     1681 caaatcaact gatctttcat cgcttgttgc gcctcttcaa gagttagatg atacaactcc
     1741 agtacttcgt cttcatcttg tgctaaagga ttctcgacct tctctacccc ttgtgcatga
     1801 taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat acatcgaagt aagatggctc
     1861 agcgacttcg cacgatagcc agtttcttct tctaattctc tagctgctgt catttcggga
     1921 ttttgtcctt ctcctggatc gatttttcca gctggaatct ccaaaatcac tttttctaaa
     1981 ggttttcgaa attgtttgac aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg
     2041 acaccaccag gatgaaatac cagttctctc ttcgaagttc ctccatctgg caaacgaact
     2101 tcgtctacag caacatcaat aatttttccg ctatagattt ctttgcgact gattgttttt
     2161 tcttcaaatt gctcattgtt cagcatatct tctcctgctt tctttgtaat cataactgct
     2221 tttttttcat ttttcaattt ttctcaaaaa ctccttatta gtttaccata gataaacctt
     2281 agattttctc taaatataga aaatccttct ttagtccgat actctttcta gtagtccgta
     2341 tgctaaaata agtatgtaat cgaataacga aaagaattca atactcaacg ataaatgaca
     2401 tggagggttt attatgataa aacgaaattg gccgtggctt ctagcattgg tcttgctatt
     2461 tctctttgtc cgaaacaata gtttttcttt tgttctagga cttttattga taggtgtagt
     2521 tttagttttg tggggactct ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc
     2581 tgcactgact gacgaacttg aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat
     2641 ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa
     2701 tatgcagcag acagccaaac ttaaagcaat cgatctccgc catgatccag tcaaagcagc
     2761 gaaagcttta ttcaaagaat tggtgaaaga accaaaccga ctgcatgaag caagtcagtt
     2821 tctttatacg catctgccga atctagttga tttgacaaat aagtataatg agatcaatga
     2881 ccatgagatc aaaaacaaac agacctatga aaaactagaa gaaagcgcgc agatcatcga
     2941 ccagctgtca gctttaatcg cacaagatta tcaaaaattc gttgctgagg atctagatga
     3001 tatcgacgta gagatatctg ttgcgaaaca gagcttaaag cgtgataata aatatgaacc
     3061 agaacaaaag gattaactct tttgttcata aaagaagact cggaacattc agatgatgtc
     3121 tggctgtttc gagtactttt tctaaagtag agacattcat gtaagaatcg agaacagatt
     3181 caacaaggag gaatttttta tggatcataa accgactgaa acaaaagatg ttacaccagt
     3241 taacgataca cttgaagatt tattgaacaa cccgttttca actccaacag atacgctgac
     3301 cacttcacaa caagcggaga ttgatcagct ccaagagcaa cagacagctg ctcgtttaat
     3361 tgacaaacta ccggcggaac gccaagaaca agctaaacag ctagcagcaa aaatcgatgc
     3421 taatgatgca cagtctgtca tcagctacgg atcggctgcc caagcaaaat taagtgaatt
     3481 ctcacaatcg atgttgaatc atgtgcaagc acaagatatt ggtcctgtag gagattcctt
     3541 aactgaactg atgtatcgtc tccaagaagc caatcctgac gaactgcgtg ccggagaagg
     3601 gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg atctatgaag tgacagcaaa
     3661 atatcaaaag attggtgcac aaattgacaa gatttcagtg aaattagata aagaaaaaga
     3721 cggtctgcta aaagataatc tgatgttaga acagctttac cagaaaaaca aagactactt
     3781 tgatgcactg aatatttata tcgctgctgg agaattgaaa atggaagaac ttcaaacaac
     3841 gatcattcca gaagcgatga aaagagcaga agaaactggc gatcagatgg atgttcagat
     3901 tgccaatgat tacacgcaat tcctagatcg tctggataaa cgtacgcatg atcttcgctt
     3961 agctagacag atcacgatcc agcaagcacc acaaatccgt ttgatccaaa acacaaatca
     4021 ggccttggct gaaaagatcc aagcatctat cgctacagcc attccgctgt ggaaaaacca
     4081 agtcgtgatc gcattgacct tgcttcgtca aaaagatgca gtaactgctc aacgccaagt
     4141 ctctgaaaca acaaacgact tattgaagaa aaattcagaa atgctgaaaa tctcagccat
     4201 cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc gagacccttc aaaccactca
     4261 aaatgatttg atcgaaacaa tccaagagac attgcgtatc caaaaagaag gaaaagaaaa
     4321 acgtcgtcat gcagaagtag agcttggaca catggaagaa gatctgaaac aaaaactgtt
     4381 ggatttaacc caatagatca cctttcgctt gttactaaaa caaacgccca acaacagttt
     4441 attacaactg ttgatgggcg tttttcttat ctttcttcta acttctccat tggagtctat
     4501 ttctcattca tcttgctgtg tccatagcca tagccaaaat aaatgatcag tccaataacc
     4561 attacgatac cgaacacgat ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa
     4621 gtcaagatgg agacgattgg caatagcgga acgaatggca ctttgaattc tccagctttt
     4681 ggtttgccaa acatttttct caacttcagt aagcctagac taacaagcat caaataggcg
     4741 agcgccatga tattcgttag ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat
     4801 acagctgcca caccagcggc ataagtcgct tttttcggtg tacgatgctt ttgatcgatt
     4861 tctgtcataa acttaggaag taagccatcc ttgctgacag caaacagtaa acgagcaagt
     4921 gaatacatca tcgagatcgt taccgtcagt agcgttaaaa ttgcccctac agcgatcacc
     4981 gcgcccactg ttccatgtcc tacatacctc attgcaaaag ccactgggtc attgactcct
     5041 agttcagtat acggaacgat tcctgtcaag atcagcgtca cgatgatgta aaggacagta
     5101 gcaatcagta tcgaaccaat gattcctctt ggcacatctt tttgcgggtt tttgacttcc
     5161 tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa aaaacacaag agctgcacca
     5221 tctagcacac cggaaaaacc aaaaggcatg aatggctgcc agttatcagg tttaacgaaa
     5281 aatattccta caatgataaa caacgcgatg ataccaaatt tgacatatac catggcatta
     5341 ttcaaacgta atgctttctt ggcttcaagc gacacgatgt aagtgacgaa tatcactacg
     5401 agcaccgcaa tcaagtcaat gtatgtacca ttttccggat tgtacccgcc agttaatgct
     5461 tgtggaagtt cggcccctaa acttttcaaa aaaccttgca tatagccaga ccatccggaa
     5521 gcaacagagg aaaccgctag taaaaattcc ccgatcaaca accaccctgt cagccaggca
     5581 gcaaattcac caaatacaac atacagatag gcatatggcc caccaatcac tggcactcgc
     5641 gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa cgattgcagc taagacaaat
     5701 gaaagtgaca gtgctggacc agcattttga ttggccgcaa cccctgtcac cacaaatatt
     5761 cccgtaccga tgatcgcacc aattccaagc atgatcaaat cagacgtttt tagttctttt
     5821 ttcatctcac tcgtatgatt gagattgatc tctttcttac gaaacaaatc cattgaagtt
     5881 cctccccatt ttaggttgga aaacagcgat taaactgttt tcatataccc taaaaggata
     5941 ccacctaaaa tgactaaaag gcaaccaaca ataacataaa tgagctcttt tttcgttttt
     6001 ctttcgccaa ggatgaaaat cccacctaaa gtagagataa tgatccccat ttgtgaaagt
     6061 gagaagccaa cagctaaacc aagtgatttg actgttagaa gcatgcagat gtttcctaag
     6121 ccccataata atccacagat catgtttttc cacacaaact gatcgacttt aactttacgg
     6181 aaagcaaaga agcttgcgcc taagatcatt ccgatacttt gaggtaagat aattgacatc
     6241 gcatccaaat tcgcccagtt gacgatgatg gtatatacac cataaccaat cgtagagaag
     6301 atcaatgctc taaaaccctt accaaaatca agcattttat tatctgtatc tatttttcct
     6361 tcgtcatctt gtcgggcagt caaatacgca ccgcttacaa gaagagctaa agcaataata
     6421 ccgtatacat aatctttcgt ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta
     6481 ttcaaaatta attgaaaacc agtagacaat ggtagcccta cagaaacacc catgtacttc
     6541 attccatgga actgtccatt ttgtccaaca ctccaagcta agccggataa aaatccgatc
     6601 aagaaaatcc aaggggtgat tacaggacta acaacgaaaa agacaactaa agaaaacaga
     6661 aatgcaccga tcgtcattcc tagtgtttgt tgattggcat cgccaccaat tttaccactt
     6721 actaaaccaa tactacccca tgccaacata gggactaatg ctattaaaat ttccataacc
     6781 aatctccttt tatttcacat gataagaaac ttatcagcga aaacgttaac aacacaagca
     6841 ctttttttcg tttctaacga ttttctaacg tttttcatta taaaaattta agattgggaa
     6901 aaataaagtc attgttgtcc cttttccttc agttgattca gccgtcaaat ggatacctaa
     6961 tttttcggcc agattttttg ctaaataaag tcctaatcct gttgaatgtg ttttgctaag
     7021 tcgaccattt ctcccagtaa atcccttatc aaagatccgt ttcaagtctt ccgctgggat
     7081 accgatccct gaatctttaa ctgaaaggct gactccttgc gcttcctttt ctatgctcac
     7141 aatgatgttt ccgtgatccg gcgtatattt gatggcatta ctaagcaatt ggttgaagat
     7201 aaaagcgatc catttagcat cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg
     7261 aatatttttt tggataaaat aatttgcctg actgcgtatc acaggctgaa tgattccttt
     7321 tagtccatat tcttggatga ggtagtcccg cgagaaactg tcgagtcttg cataatacaa
     7381 aacttgttct acgtattcat cgatttttga taattcattt tctagtaatg tatacttttg
     7441 atcgtcaata tcgaactcga tcgaacgaac tagcaattta gaagcggaaa gtggcacttt
     7501 gatttcatgg acccatgagt caatataatc tttttggtct tgttgattac tgattgctga
     7561 ttgcattact tcttggtgtt cacggatcag ctgattgata tattcttcta ccagtttttc
     7621 ttcttctgtc ctcccaccac tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc
     7681 taattttccc caccatcttc tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa
     7741 gacgccctca atcaaagcta aataagcaat tgtgctcaaa tcaactttga tgtttggcgc
     7801 cagccacatc acaaagatcg tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca
     7861 ctgatctttc aaatatttaa aaaaattcat cttaaccgct catcctttca gggaacgata
     7921 tatccctgtc ctacttttgt ttcgatatat ccttggatac ctgcctgttc gatttttcgg
     7981 cgtaaccgat tgatgttgac tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac
     8041 agtgctcgca gcaatttttc ccgacttaag atctttccat tgtgcttcat caaaataaat
     8101 aatagacgat attcattttt gctcaaatca atgatctctc cgttgatttc catacttccg
     8161 ttgtcaacat tcaacgtaat gccattgtgg ctcatcattt cactagataa ttctgtgtag
     8221 ttgtaagaac ggcgaagtaa tgcattgatt tttgcaatca gtacatcgat ttggaaatgt
     8281 tttgtcacaa aatcatctgc ccccatgttc atagccatga tcatatccat attcgtattc
     8341 ctgctggaga taaaaatgat cggtactttc gatacttcac gtattttttg acaccaataa
     8401 tagccatcca aaacgggaag attaatatct agtaatacaa gctgtggttc ttctctttca
     8461 aagtctgaga acacttgatt gaaatcggtc gttccaaagg tttcaaattg ccatttctca
     8521 agttcctcca tgatcaattg tcgaatgatc tcttcgtctt cgactaccat gatttttgcc
     8581 ataaaaaatc cctccatctt tcttcttgct actacaatat caaagaagcc cttgtgaaac
     8641 aagttggctt acctggaaaa tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg
     8701 atttaatcgc gtttgtctct tccaagcagc ttaaatttat tatgttattc ttaataattg
     8761 gtctattatc tgttattttt ctttcgttgt ttttgtcttt taaattttcg ataacaccaa
     8821 taaccgaaaa gcgttagcac tagcaatata cctagaagga gtataagtaa tcgataaaga
     8881 tggtattttt ttgtctgttc tatctttgaa gagacttctt ctgtaaaggg aactcgtctt
     8941 cctgtcacta gtaatcggtg agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact
     9001 agatcctgct tttctctaat tgctaatgta tttgtttttg ttggaaggac aacaattttc
     9061 tcaacgactt cataagcgag catccgacca tacacttcaa gatagaatcg gtcatcttgc
     9121 tccatttctt caagatccgt aaagagtttt ttctcagcaa taccgctatg ggcggtaatc
     9181 acggaatgtg tactctctcc tccagttgga aaagaggtgc cttgcaggac tgtcgctcct
     9241 ctatctaaca aaagatcatt cgtttcatca aataaaggca agctaacatt gattttcgga
     9301 atatagatgg ctccgatcat atgttgttcg taatattctt ttcctggatt ttggacattt
     9361 ctaaccgctt gctcaaaagg atctttgact tgtcccattc ctgggattac tgatgtatga
     9421 gcaagcgtct gatttttctt ttcatgcact gctaattgtt gctcatgttt ttcttcttta
     9481 tcgcgttcaa gctgcttttg ataattctcg atccttcttt gatccaaata attgctgaga
     9541 gcatctgcta aaaatggata tgtaaaaact gcagctccgg caagaaacat cactgccata
     9601 aataattgta ataaaagact gatttgggtt tgaggtctat tacctatttt ctttttcatt
     9661 ttcttcacct tcttatttaa aaaaactgca gcaaccttta taaaaccgat tgctgcagct
     9721 tatttttgtt gcttatactt cagacttcat tttagattta cggtaccagc tgtacgctcc
     9781 aatcatcaaa cttattccga ttagtaagaa tgccaagagc ccgttcccac cagtagcagg
     9841 aagtgacccg cgtttcatat tcggtactct ttctggccct ggattttcta ctcctacgat
     9901 ctgaatttct tcattatagc ttccataaga gatgataaat tcagtaaagg ctgcctcacc
     9961 tgttggaagt acataacctt ctggcgccat ggtctctttc atttgatagg ttccataatc
    10021 taacccttga acctcaaagt atccatttcc atctgcgaca aattctgttg cttgtccgct
    10081 tgttgcccac tcaattctat ctgctcctga ttcataaaca gctaaaggcg actccccttt
    10141 ataaaatatt gcgtattcag aacggtcttc attccacaat tcgaatcttg catctttaag
    10201 caattcactg cttgatgcat catatttttc aaactttctt ccacctgttg tgactttctc
    10261 tggcggctct actccttctt cataatcaaa ttcatcattt cgtccgattt caataaccgc
    10321 tgtgttgtta tggaaatcat ccgcttctag ttctgtggaa accttcaaat tatacgtgat
    10381 cgttaaatct cctccacgtg catctgctaa tctacctaaa gtatctactt tggctactcc
    10441 attttcttct gtcaaaatcg tcacgacaaa cccgttgctt actacttcta ctatataatc
    10501 ttgtcctttt gttaaaatcg tctcgccggc tcgcacttct atttcctgat cataataagt
    10561 tagaccaact gtaggcgtat ctgtgatttt gtaatttttg ataacagcgc ttccgttttc
    10621 aaggacagaa tcgatgaaat aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc
    10681 tctttctaga tcaatataag aaatcgtttc ttgttctcca gcttcattaa taatcgttac
    10741 ttgtctaagg tcgctttcct ctacattcat ctgtttatct gcttctctga tttcgttttt
    10801 cggatacaaa tgcacatctg tattgtatgt gtctttccat gtagaaccat cccacacgcc
    10861 atccaccata tcaggcatca tcactggcat cataacaacc attggagacg cgattctgga
    10921 aatatgagct ggcgaataag tttctaagaa aagatagact ttatctctag aagaaggtgc
    10981 ttcttccgtc acttggacgg tagagaatac cgcttctcca gcttcattcg ttgtttgttt
    11041 gtcaatactt gtcagtcgat attttaaaaa ccaactttct gtgtgccgtt tttgaatcca
    11101 ttcaatcgct tcggcagaat taagaccgtc ttctcgttcc ggtgttaaag gattatctcg
    11161 aatcaaatcg taatatactt ccgttacgtc aaacaaatca aaatcgactc ctggaatatt
    11221 ttcttcacca aaatccgtag aaaccaatcc gctattcggt cgttcttccg gaacagatga
    11281 gaaacctttt ttgtgtaatg taatcgtcac ttcacttggt ttttgtgcga cttcttcggc
    11341 tttgactagt tttccgttcc cgaaaatccc tattcccgca gtaacgagta acagtacact
    11401 cattcccgtg acaatcattt ttttattcat ttttttctcc ccgttctttt tttattaacg
    11461 ccgattgtaa aattaagcta gacaactaaa aaagtcattt gtgctacact caaaatagtt
    11521 ccgaccaaag aattataagg agtgagtaca aatgacctat acccatctta caacggatga
    11581 acttgtaata atagagtctt atttcaaaat gaatcaatct gttgctaaaa ctgcccattg
    11641 cttgaatcgt tcaagacaaa cgatccataa agtatatctg ttcttcaagc aaggaaaatc
    11701 agccttagaa tattatcaac agtataagaa aaacaaatca aactgtggta gacgtccgct
    11761 tgttttaccc gaggaacaat cagaatatat tcaaagaaag gttgttcaag gatggacacc
    11821 cgatgtgatt gttggtcgtg cagcgtttcc tattagttgt tctgctcgta ccatttatcg
    11881 tatgttcaaa aaggggctat ttgattcttc tgacttaccg atgaaaggca agcgtaaacc
    11941 gaatggacat caagaaagac gtggaaaaca aactttccgc cgctctattc atgaacgtga
    12001 aaaggattat agccaattct caaatgagtt tggtcacctt gaaggtgaca ctatcgtagg
    12061 tctgaaacat aaaagtgctg taattacctt agttgaacga ttatcaaaag ttatcatcac
    12121 attgaaaccg tgtggtagac aagcgattga tattgaaaaa aaattaaatc aatggtttga
    12181 atctgtaccg aaaaacctat tcaaatccat cacttttgat tgtggaaagg aattttcaaa
    12241 ttggaaacag atcagtaatg tcaatgatat tgccatttat ttcgctgatc caggaacgcc
    12301 gtctcaaaga ggcctaaacg agaattctaa cggattgtta cgtagagatg gtttattgaa
    12361 atctatggat ttcaattcag tagatgaatt ttttattcaa tctgtcgcat ctaaacgaaa
    12421 taatattcct agaaaatcac tggattatcg aacgattatc gaacaccttt ggaagtattt
    12481 ttgagttacg taagtattga tgatctgtct aatttaattt gacaattaag aaagtattaa
    12541 cttgttttgt gaaaaagaga atttgaaaaa cgattggcga acaaatgctc ggtaggtatg
    12601 gtataaatac agagctaatt ggtgtgatga aatcagatat tcgcgtgaga ttcagtaaag
    12661 taggagttcg aaaacagcta ggtgacaaac agtattgctt tggtacttcg gaatggttta
    12721 tcaaattctt atcaacagtt cacttctctt tatgtttgag tgatgattcg ttaggttatt
    12781 gcttggaaag accggatgag agtagaagag tttaatttta taaagtgaaa agaggtaatg
    12841 tacgatatac gtagggctga accaatagag gataagcaaa cgatgttaca ggcgcaaaat
    12901 tcatagctta ctgttttgga gataacagaa gaggaaataa tgcagaagta aggcgaaaaa
    12961 aggacggaag cctaacagca tatgaaataa agaaaaatat tataactact gataaatcgg
    13021 tattgagcaa agtactaata ggagtcaaaa acattcataa gctatgagtg tttcttggct
    13081 acttttttat ttttatgtat ctacaaaagt ttccaggggc agaacctgtg attattaatg
    13141 gaagaacctc aagtattgtc ttaaatcatt gttatgaaaa tggattaaaa gagttcatca
    13201 aggtgtgatt aacaagttac ttttcattac atcgaatttt tcagaagttg cttatattgg
    13261 tgatgatatt aatgatttat catgtatgac atctgtgaag aagcgggagg aatagtagaa
    13321 tgtccaaaaa atgccacaag aaatgagatt gaattatctg attttattgt aactcacagt
    13381 ggtggtgacg aatctgtgat agagtttatt gagtggatat tggaatcgat atagtgtagt
    13441 agtacagaaa aattatcata caattaatac taggcataag aatattggca tattctagag
    13501 ttaaaagtaa ctattcgaaa aggataaggt tagaattttt tgttttgcca gtggaagaat
    13561 ataaaaaggg cattggaaat taatatatta gaatgctaag cggagaaaaa aatatatgaa
    13621 aagtacgtcc ttgtgatggg atggaaaaat acacttattt caaacaacga taatataatt
    13681 atgtgaaccg aagtatactc gcttctaatt caatcctcaa atcccgacag tcgcgacttc
    13741 aaaaaagcgt atagttttaa tgtatcccca tatgatccga aaaatggtaa tttacaatta
    13801 acagtttgat gatgttgaag atgtggaaat gctgaaatta ccatgatgag aaacacatta
    13861 attaaaggga attaattaac tagaatataa tctgattcaa tttgaattaa gcaaaaaaag
    13921 gctgagtttt aaat
//
LOCUS       DACDMM010000065        13660 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDMM010000065 DACDMM010000000
VERSION     DACDMM010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.065"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..748
                     /locus_tag="IWD16_001508"
     CDS             <1..748
                     /locus_tag="IWD16_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9088571.1"
                     /translation="HYHAFIRKALQEAVITELISTNVADKVRKPKKKQFVSQVYNQQE
                     ILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAIDFLNKKIMINHVIIENPENM
                     SQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQDNRILLGDDYILEDNEYVFTME
                     DGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASIMLDSGQNMKSIQEWLGHASY
                     STTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1322..1891
                     /locus_tag="IWD16_001509"
     CDS             1322..1891
                     /locus_tag="IWD16_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP9088572.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1878..2738
                     /locus_tag="IWD16_001510"
     CDS             1878..2738
                     /locus_tag="IWD16_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9088573.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2928..3632
                     /locus_tag="IWD16_001511"
     CDS             2928..3632
                     /locus_tag="IWD16_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9088574.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3637..5472
                     /gene="walK"
                     /locus_tag="IWD16_001512"
     CDS             3637..5472
                     /gene="walK"
                     /locus_tag="IWD16_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP9088575.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5469..6785
                     /locus_tag="IWD16_001513"
     CDS             5469..6785
                     /locus_tag="IWD16_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088576.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6786..7655
                     /locus_tag="IWD16_001514"
     CDS             6786..7655
                     /locus_tag="IWD16_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088577.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7710..8519
                     /locus_tag="IWD16_001515"
     CDS             7710..8519
                     /locus_tag="IWD16_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9088578.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8622..9251)
                     /locus_tag="IWD16_001516"
     CDS             complement(8622..9251)
                     /locus_tag="IWD16_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP9088579.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9421..10713
                     /locus_tag="IWD16_001517"
     CDS             9421..10713
                     /locus_tag="IWD16_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9088580.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10789..11451)
                     /locus_tag="IWD16_001518"
     CDS             complement(10789..11451)
                     /locus_tag="IWD16_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9088581.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11676..11960
                     /locus_tag="IWD16_001519"
     CDS             11676..11960
                     /locus_tag="IWD16_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP9088582.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12011..13636
                     /gene="groL"
                     /locus_tag="IWD16_001520"
     CDS             12011..13636
                     /gene="groL"
                     /locus_tag="IWD16_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP9088583.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4591 a         2322 c         2915 g         3832 t
ORIGIN      
        1 tcattatcac gcatttatac gcaaagctct tcaagaggct gttattacag aattgataag
       61 tacaaatgta gcagataaag taagaaagcc aaaaaagaaa caatttgttt ctcaagttta
      121 taatcaacaa gagatcttga aattacttga cattttgtca gaagaaaaac tctatttggt
      181 agttctttta acagcatttt atggattgcg aagaagtgaa gttttaggat taaaatggtc
      241 tgctattgat tttttaaaca aaaaaatcat gataaaccat gtaatcatag aaaatccaga
      301 gaatatgtca cagttaataa agaaagatca aacaaagaac tcttccagct atcgtacatt
      361 acctttggtt gaaacgattg aacgagcatt agtaaatcaa gctaaatggc aacaagataa
      421 tcgtattctt ttaggagatg attatatttt agaagataat gagtacgtat ttactatgga
      481 agatggacgg ttgatgaagc ctcaatatgt aacgcatcgt ttgagtaagt tgattaagaa
      541 aaatggattg aaaaaaattc gctttcatga cttgcgtcat tctaatgctt caataatgtt
      601 agatagtggc cagaatatga aatccattca agaatggctg ggacatgcat cgtattcgac
      661 aactgctaat ttatacacgc atttaaccgc aggagtaaaa gatcgtgcag cagaagcatt
      721 agaaaacgca tttgggttta aagaataaaa aaacaaaaaa caatatcttt ggggtatcgc
      781 ttcttgtttt tagaattttt ttgttggcaa tttgttggca gtatgttggc aatgaaaaaa
      841 ttcacaaact aaaaacctat ttataaagct ttataaaact tttttcacaa atagagcatt
      901 tggttaagag tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata
      961 atagtagcac taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt
     1021 tacttcctta taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat
     1081 accaacaaac aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc
     1141 tttcccgccg tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag
     1201 ccttcttgtt ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta
     1261 gattgaatgt ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat
     1321 gatggaaaaa atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct
     1381 gcctcactgg gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt
     1441 tgatcgttat ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat
     1501 ggtaaacaat tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa
     1561 agaacacgta gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga
     1621 aatcaaagaa gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct
     1681 attttgcgat gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc
     1741 tcatccgcaa aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac
     1801 tttatcgttt gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt
     1861 aaaagaggag aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg
     1921 atgttccaga agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt
     1981 ataaagatca ggtatataca gataaaattg acattacccc tgaagagatc tatcaacgtt
     2041 tgccagagga aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg
     2101 agcaaatcaa aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga
     2161 gcgggacata taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg
     2221 tactagatac gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat
     2281 ggctggaaga aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa
     2341 gaacaaaagt tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg
     2401 gtttagttgc ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg
     2461 aagaagggat ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa
     2521 cggttgctta cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc
     2581 atggggatgc gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac
     2641 aagcaaacca aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg
     2701 gattattggg tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag
     2761 gtgaaaatca gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg
     2821 cactttcatt ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata
     2881 cggtataatg agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt
     2941 tagttgtcga tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag
     3001 gctatgacgt ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag
     3061 aacctgattt gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc
     3121 gtgaggtacg caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa
     3181 ttgataaagt cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca
     3241 accgagaatt agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg
     3301 aaccagaaga ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag
     3361 cttacatggt gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac
     3421 tcttttactt ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg
     3481 tttggggtta tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc
     3541 gtgaaaagat cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg
     3601 gttactattt gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt
     3661 cgatcagtca attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa
     3721 attattggtg cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa
     3781 gatatgaatc aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa
     3841 gataatgcag atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg
     3901 actagcgata tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc
     3961 gatgtcaatg aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc
     4021 acgactaaac gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa
     4081 ccgatcctgt ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc
     4141 gaacaaaaat attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt
     4201 gcggcattta tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc
     4261 ggtgaaatga gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa
     4321 gtgcatggac aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt
     4381 attgaagaag ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc
     4441 catatgacag acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat
     4501 atggctgttt ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca
     4561 cttttagata tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg
     4621 gtacttgacc gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt
     4681 tctatgatcc gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg
     4741 acagaacaag aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa
     4801 cttcgtacac cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg
     4861 aaagacgagg aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg
     4921 attcgaatga tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt
     4981 caattagaat atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg
     5041 atggtgggaa atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt
     5101 tgggtcgaag tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg
     5161 atcaagtatt caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac
     5221 gtgattttaa gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg
     5281 ttcgagcggt tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt
     5341 ggattagcta tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc
     5401 aaggaaggaa aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa
     5461 tggtgggaat gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt
     5521 taagcatata cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg
     5581 tagatgagac aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct
     5641 ttcttccgct tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa
     5701 atctcgtgta ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag
     5761 tagtttcagg agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt
     5821 tggcctataa tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag
     5881 atgtgtcgaa cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc
     5941 aaggaaataa aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata
     6001 tttcgttgaa ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag
     6061 aaatgacaac agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac
     6121 tgaagaagta tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt
     6181 tccagaaacc agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg
     6241 ggaatgaaac aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc
     6301 cagagaaaaa agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag
     6361 gcagttcttt agggaacctc agatattttg accgcagtga caatacgatc gattatcgga
     6421 tattcgtgga aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag
     6481 ttggcggtga aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc
     6541 aagtgccgat accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc
     6601 agttaaaagc taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat
     6661 ggcaaaatgt caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat
     6721 ataaagataa ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga
     6781 attaattgga tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg
     6841 tgtttttatt cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta
     6901 atcagactga atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga
     6961 tttcgtctga gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt
     7021 cattagcgaa ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag
     7081 acggtgatgt tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc
     7141 cagataaaat tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg
     7201 gtaatgatta tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc
     7261 aagcaagcca agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt
     7321 ctttagaaaa aaacgataat gattatagca tcacgaaata catgcagacg catttatctg
     7381 atatcgagca gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat
     7441 tgtacatcaa taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt
     7501 cctgtattct gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg
     7561 aaacaagcga taaagtggtc caaattgaga gcgtcaacgc tttcagcaat acgatcgtga
     7621 caaacaatac tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt
     7681 tacaaagtaa gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca
     7741 gtatcctagc aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc
     7801 ttttggtcga tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc
     7861 ggacaccgga ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg
     7921 gcgtgggtgt cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg
     7981 aagcgatgga accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg
     8041 ggaaagtcag aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag
     8101 cggctccgca attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca
     8161 ccggatattg cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg
     8221 aaagtaatca tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc
     8281 gtattttagg agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg
     8341 tgatcggtaa tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga
     8401 aagaacttgc tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac
     8461 atgattttga aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa
     8521 cagttcaatc aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc
     8581 ttctattttt atttttttag catattaaaa aaataaccgt attatcgact gactacactg
     8641 atgtgctgct tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct
     8701 gccatgctga tcgtactgtc gctagcagcg tttctttgta aataatcatc acttaacaaa
     8761 taagtacctg tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa
     8821 tgtagatttt tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga
     8881 tagaagggtg catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag
     8941 aataacgaag aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca
     9001 atcaaatggg acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca
     9061 tcaacgacaa catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt
     9121 ataaacggaa catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa
     9181 tctctggaca atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt
     9241 gcaattttca taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat
     9301 tcgtcaaaga atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca
     9361 ttgggtatag ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa
     9421 atgaaagagt ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga
     9481 cccttaactg ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg
     9541 ctgtttttct tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca
     9601 ggattttcta tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg
     9661 atcgtttttg ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc
     9721 ggcataacag ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat
     9781 actgtctgga caggtgctga agggatacta ggagcagtta caactgggct catcatcgga
     9841 tggggctatg cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct
     9901 gaaaatgttt ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg
     9961 cttatcggtt cagcggtttt tcaacatttg ttcagaatga ctgttgttca aggagtatgg
    10021 acatatgtcc agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta
    10081 ggtttttttg ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg
    10141 ttggttagtc ccgtcttgca agcgaattca ttagcaaatg cagaaatctt agcctcagga
    10201 caagagttga cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc
    10261 atgacggtta cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg
    10321 aaatcgaaga aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc
    10381 aatgaatcaa ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt
    10441 attttcgcac cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt
    10501 cctttattta cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta
    10561 gtaggcggag tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt
    10621 tatttcccat ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa
    10681 aatgatggag cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag
    10741 attgggaaaa tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc
    10801 tttttggaca gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat
    10861 cgatgtccag atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa
    10921 gtagattagc agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc
    10981 tccgacccaa aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa
    11041 ctcttccatg atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat
    11101 catctggatg atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc
    11161 taacatgaca gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat
    11221 aacaatcttt ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg
    11281 cttaaaataa aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt
    11341 gatcaattca gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa
    11401 aattagacca tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct
    11461 ccttttaagg gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca
    11521 taatttcaat aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa
    11581 aaagagggag aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta
    11641 acagattaaa aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg
    11701 tcattatcga agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg
    11761 ctgctaaaga gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag
    11821 aaaacggtga aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat
    11881 acgccggtac agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata
    11941 tcatggcgat tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg
    12001 tgaatcaatc atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg
    12061 cggggtagat aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt
    12121 cgtactagaa aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa
    12181 agaaatcgaa ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc
    12241 atcaaaaaca aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc
    12301 aatcgttcgc gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg
    12361 cgggatcgaa ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt
    12421 tgattcaaaa gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg
    12481 acatttgatt gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga
    12541 atcaaaaggg atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta
    12601 cttatcacaa tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata
    12661 tatcttgatc acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca
    12721 aatcttgcaa caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt
    12781 gccaactctt gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc
    12841 tggtttcggc gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac
    12901 agtaattact gacgatctag gtttagaatt gaaagatgca acaatcgaaa acttaggaaa
    12961 tgcttctaaa gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa
    13021 agaagcaatc gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga
    13081 ttttgaccgc gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt
    13141 taaagttggt gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc
    13201 attgaatgct acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct
    13261 agtgaacgtt atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat
    13321 caagatcgtt gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta
    13381 tgaaggatca gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc
    13441 tgctactgga gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac
    13501 acgttctgcc ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt
    13561 cgttgcagat aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat
    13621 gggcggtatg atgtagtaat taagaaagaa ccctatttta
//
LOCUS       DACDMM010000066        13613 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDMM010000066 DACDMM010000000
VERSION     DACDMM010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.066"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            139..477
                     /locus_tag="IWD16_001521"
     CDS             139..477
                     /locus_tag="IWD16_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088584.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="IWD16_001522"
     CDS             complement(682..1257)
                     /locus_tag="IWD16_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP9088585.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1989..2570)
                     /locus_tag="IWD16_001523"
     CDS             complement(1989..2570)
                     /locus_tag="IWD16_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9088586.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2753..3379)
                     /locus_tag="IWD16_001524"
     CDS             complement(2753..3379)
                     /locus_tag="IWD16_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9088587.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3401..4732)
                     /locus_tag="IWD16_001525"
     CDS             complement(3401..4732)
                     /locus_tag="IWD16_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP9088588.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4887..5288)
                     /locus_tag="IWD16_001526"
     CDS             complement(4887..5288)
                     /locus_tag="IWD16_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9088589.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5374..5580)
                     /locus_tag="IWD16_001527"
     CDS             complement(5374..5580)
                     /locus_tag="IWD16_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088590.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5675..7177)
                     /locus_tag="IWD16_001528"
     CDS             complement(5675..7177)
                     /locus_tag="IWD16_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP9088591.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7195..7650)
                     /gene="argR"
                     /locus_tag="IWD16_001529"
     CDS             complement(7195..7650)
                     /gene="argR"
                     /locus_tag="IWD16_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9088592.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7748..8365)
                     /locus_tag="IWD16_001530"
     CDS             complement(7748..8365)
                     /locus_tag="IWD16_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088593.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8586..9959
                     /locus_tag="IWD16_001531"
     CDS             8586..9959
                     /locus_tag="IWD16_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9088594.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10034..11146
                     /locus_tag="IWD16_001532"
     CDS             10034..11146
                     /locus_tag="IWD16_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP9088595.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11218..12855)
                     /locus_tag="IWD16_001533"
     CDS             complement(11218..12855)
                     /locus_tag="IWD16_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP9088596.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13023..>13613)
                     /locus_tag="IWD16_001534"
     CDS             complement(13023..>13613)
                     /locus_tag="IWD16_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9088597.1"
                     /translation="IVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKISQKFAG
                     DNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTETATQEHT
                     YVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQLDASYLN
                     GDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3672 a         2871 c         2448 g         4622 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 ggtgttcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg tagaaagcca
     1561 ttttcacaaa atttttctta atactcgaaa ctaagcgact ttttcacaac ctcttcctta
     1621 aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa aatatgagca
     1681 gctattttta tttggctgtt cttattactt gatgcagggc agctttttca caacctctcc
     1741 actaaacggt gttcaatcag ctaaccttcg gtattagctc aaaaaacggc aaaatatgag
     1801 gaactatttt gccgtttgtt tctctaatac tcaggtcaaa gcgctgattt cacaacctct
     1861 aatacacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt
     1921 gagaagcaat tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc
     1981 tctaattatt aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc
     2041 tgcctaagta gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca
     2101 gcaatcaacg ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt
     2161 gccaggatat tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga
     2221 cggtattctt catccactgt actcaactca ataatcaaat tacctacagg acagccatga
     2281 taagtgatct gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct
     2341 tctggctgtt ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata
     2401 tgcgcatgga tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga
     2461 ccttttcctg tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt
     2521 ttataaataa gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc
     2581 ctttttcatt tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc
     2641 gtttaaaaaa gactgtgcca aattatctga cacagccctc actataaaac tgttctcata
     2701 tagctagatg ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata
     2761 gttgctctaa tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca
     2821 acgctgcatc cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat
     2881 tgaatccact ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc
     2941 caccaagaat tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt
     3001 ctggtccaaa gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca
     3061 attcttcgat aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag
     3121 caaacatctg ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt
     3181 catgtgggta aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt
     3241 ttaccatttc aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac
     3301 caaagccacc gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg
     3361 tatttttatt attgaacatg gtggattctc cattcgttat ttattttaat acatcattta
     3421 gagcttcact cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt
     3481 ttccattcat agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac
     3541 ttgcccctag aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat
     3601 cttctaatac ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga
     3661 cgccttgtgc tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa
     3721 atacactgta aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact
     3781 gatccataat gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga
     3841 cgtctccaat cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg
     3901 ctccgcgatc tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt
     3961 ttcttccagt tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg
     4021 taaaggtgac ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat
     4081 ggaattcgat tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt
     4141 cacgtggaag aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga
     4201 acattgatgc aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta
     4261 attgttcctg atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt
     4321 tgattggtaa aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa
     4381 tcactttttc accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac
     4441 cgtccatcac tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca
     4501 tgcccgtcaa tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat
     4561 tgaaaatcaa tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac
     4621 gtgttgactt ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag
     4681 tcttaccgcc ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct
     4741 cgcttctttt ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc
     4801 caaatcatct gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt
     4861 agtctggttc gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct
     4921 ctagttgctt tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct
     4981 tcttcgctga tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca
     5041 tctaaattat aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct
     5101 ttgatcatat gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt
     5161 tctggatttg ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca
     5221 tttggtgcct cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct
     5281 tttttcattg tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta
     5341 tgacaagtaa ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc
     5401 tactattttt ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc
     5461 aaaataaata gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata
     5521 taccagaaag ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat
     5581 tttttcctcc ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg
     5641 ccgtccaagc aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat
     5701 cagaaaaatg tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt
     5761 catcattttc tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc
     5821 agccgtttcc gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga
     5881 agttcatccc tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt
     5941 gttcgcgtgg ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt
     6001 tcaactcttt gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca
     6061 tcaggatctt tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag
     6121 cgtgtccgct tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg
     6181 ctttattgat tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt
     6241 caatgaatct ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat
     6301 gctcttttaa tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc
     6361 cagaagcact gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg
     6421 cttcatttgc taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg
     6481 ggatatgatt aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac
     6541 ctaagctatt caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa
     6601 tgattttcca accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg
     6661 tgactaaatc ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt
     6721 cgctttttat tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc
     6781 cttctgaggt aaagaatttt attccattat aataataggg aagatggcta gcagtcagca
     6841 taaccgcagc atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag
     6901 tagctaattc gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct
     6961 ctttcagtgc tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat
     7021 ttggatcggt tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt
     7081 ttaccatgtc tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa
     7141 tatccgatcc attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc
     7201 cctcattttt tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat
     7261 aatgaatgta tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag
     7321 ccaattcgtt accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac
     7381 agactggata cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga
     7441 aacagatggc tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac
     7501 taacttcatt tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc
     7561 atgcagccgc gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg
     7621 gatcagtgct tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca
     7681 aaagataagt aatatttctt catattttgg tattttttac ttttattttt tgttttcatt
     7741 caggttgcta acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg
     7801 attttcttgg atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt
     7861 cttttgcgat gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga
     7921 cgtgtaagat ttgggcacaa agaccttctt ccatcttttt gaatactagt tgttctttca
     7981 agtcttccgg aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa
     8041 agtcaggctg tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat
     8101 gcaaatactt ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat
     8161 aatcaggaat cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata
     8221 gacaggctac tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa
     8281 agaatggttt tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt
     8341 ctttacgcca ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta
     8401 ttatcatacc tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg
     8461 ctagaataag tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag
     8521 gcggtttcta tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga
     8581 tgtaaatgac aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga
     8641 tgtcttttgc gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca
     8701 gacagtttga tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga
     8761 tttcgatcat tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct
     8821 ttttagcttc aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct
     8881 tcatggtcct attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt
     8941 tgatgttcca tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg
     9001 tcggtacttt gacaacggcc attgctccag caattggccc cacttatgga ggcattatga
     9061 cttcacattt tacatggaat catatcttcc tgtttcttgt gcccgttttg attctttctt
     9121 tgattatcgg ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt
     9181 ggataagcgt tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt
     9241 tgggaacagt ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt
     9301 actatcgaag taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa
     9361 cattccgctt gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt
     9421 ttgtcttgcc gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag
     9481 cgatgcttcc cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg
     9541 atcaatatgg tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc
     9601 tagcattatt cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt
     9661 tttatatgat cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac
     9721 tgaatgaaga actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg
     9781 gcgctgtggc tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc
     9841 ataactatga agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt
     9901 tgcttctcgt aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga
     9961 ttccatatac ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa
    10021 aggaggcttt tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgactct
    10081 agccagttac ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca
    10141 ggcaactaaa gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca
    10201 atggtatcgt ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca
    10261 acttgatcaa acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt
    10321 agctgaattg gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat
    10381 cgaatggctt tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc
    10441 actgcgtatc agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca
    10501 gcgtttagca caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag
    10561 cgattcttgg ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac
    10621 acctggcccg gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc
    10681 gcaaaaaggg cagctcttag catttcagac atcttataag atgagtcaag actggcctgt
    10741 tgctatgctt gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg
    10801 tgctacccat gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg
    10861 attgactaag agtacagaac agttcttgac tgatccagat cagataaaaa actggccata
    10921 cgagttccgt gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact
    10981 gccagatgat ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg
    11041 tccttatatc ggctatctat tagccaactt cttaaatact ggtcattggt ctacggaaga
    11101 ttatcaaaag gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt
    11161 caagtaagga tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta
    11221 gttcaatttc tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt
    11281 tcctatctga gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt
    11341 ataggacgta tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc
    11401 aaatggacca aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa
    11461 tgtcgtttca ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc
    11521 tgtttgctgt ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac
    11581 acggaaagga ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat
    11641 attttccgcg tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg
    11701 gtcattgatc ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc
    11761 gatcttttcg ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg
    11821 cccatttccg tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt
    11881 cacgcccaca ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc
    11941 ttgtgtttta tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat
    12001 aacgaaagca tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt
    12061 aatcgttgat tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt
    12121 actagcatat tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc
    12181 ccaagcaagc gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc
    12241 cgctgtatta tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca
    12301 ttcgggatca caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt
    12361 ctctaagatc ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc
    12421 aaatccttct ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat
    12481 tacttttgta tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt
    12541 ctcgtatttg ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct
    12601 tcccacaatg cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc
    12661 ttcagcatgt gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg
    12721 ataagctgtc ttcctgtcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac
    12781 aatcgatcga atactggata acagtggatt tccacttgtg atttcgttta attcgagact
    12841 ctcttttgta ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata
    12901 cattattcga gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac
    12961 acaaaaaaac cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg
    13021 gcttagtacc agccgtttgc taaccagaat gttttagcag catcccatga accataacga
    13081 gatgttacat attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag
    13141 tatgaagcat ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg
    13201 cctgattctt tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca
    13261 gctggggcag caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc
    13321 gttgctgttt cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa
    13381 gaatcatttg aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg
    13441 atcgaatttt tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag
    13501 atcttagata atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta
    13561 gttcctacaa acattgcagt agcaccagca gcaaaagttg tacctaaaac aat
//
LOCUS       DACDMM010000067        13409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDMM010000067 DACDMM010000000
VERSION     DACDMM010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.067"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            298..2037
                     /gene="yfcC"
                     /locus_tag="IWD16_001535"
     CDS             298..2037
                     /gene="yfcC"
                     /locus_tag="IWD16_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9088598.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2194..3378
                     /gene="iadA"
                     /locus_tag="IWD16_001536"
     CDS             2194..3378
                     /gene="iadA"
                     /locus_tag="IWD16_001536"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP9088599.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3489..3692
                     /locus_tag="IWD16_001537"
     CDS             3489..3692
                     /locus_tag="IWD16_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9088600.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3793..4455)
                     /locus_tag="IWD16_001538"
     CDS             complement(3793..4455)
                     /locus_tag="IWD16_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9088601.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4468..5970)
                     /locus_tag="IWD16_001539"
     CDS             complement(4468..5970)
                     /locus_tag="IWD16_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9088602.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6518..8197
                     /locus_tag="IWD16_001540"
     CDS             6518..8197
                     /locus_tag="IWD16_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9088603.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8324..8803
                     /locus_tag="IWD16_001541"
     CDS             8324..8803
                     /locus_tag="IWD16_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP9088604.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            9022..9702
                     /locus_tag="IWD16_001542"
     CDS             9022..9702
                     /locus_tag="IWD16_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP9088605.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9710..11041
                     /locus_tag="IWD16_001543"
     CDS             9710..11041
                     /locus_tag="IWD16_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9088606.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            11056..12018
                     /locus_tag="IWD16_001544"
     CDS             11056..12018
                     /locus_tag="IWD16_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP9088607.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12129..12467
                     /locus_tag="IWD16_001545"
     CDS             12129..12467
                     /locus_tag="IWD16_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP9088608.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12546..13253
                     /locus_tag="IWD16_001546"
     CDS             12546..13253
                     /locus_tag="IWD16_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP9088609.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4299 a         2348 c         2925 g         3837 t
ORIGIN      
        1 ttattttttt tgcaatgaaa ttcaattttt caatgattgt aaaatgaaaa aaacaggacg
       61 agattttctc taatctaaaa ataaaaaaag aattttaagg aaaaacagag aaataaaata
      121 cttaatataa atggaaagag aaaaaaataa tcatacatag gagaaaaaaa acaaaataga
      181 aaaaagtgat tttctaataa attattcatg tggaaatgtc atttttactg tataattcat
      241 ttgattatcc attttttatt catcaaaatt actgaaaaaa gaaaggaaga aataaatttg
      301 aagaaaacaa attcttcagg aaaaccgaaa cgcgacttca ctaaattatc gacaccccat
      361 acgtatgtca ttatttttgg tgtagtcatc tttgcatgga tactgacttt tgtcgttcca
      421 gcaggaaagt tcagcacgca agatattgaa tacaaagatg caaatggcga aacaagtact
      481 cgtactgttt tacgtcaaga ttccttccgt tatgcttacg agctggataa gtcgtatgtg
      541 tttgatcaat tagaagaact gcaagatcat ccagcagaac gtgaaaaact ggatgttccg
      601 gaaaaaggat tggaaaaagt cattgctgac ggagaaaaaa atcttacgca ggaaaaacta
      661 gatgaaatct ccttgacaga tgatgtgctt tacgatcagt acggcgaaaa tatctatgat
      721 acttctaaaa aattgcataa aacagccaaa atatggggaa cagatgattt tggcggcttt
      781 ggtttcttaa attttgtttt tgaaggacta gtatctggag ataagtatgg atctgctgta
      841 gggatcgctg cattgatcct tgttgttgga ggtgcttttg gtattattat gcgtaccgga
      901 gcgatcgacg caggtatcta tgcgtttatc agtaaaacta agggactgga gcgtttagct
      961 ttaccgctat tattctttgc tttttcattc ggtggcgcta cattcgggat ggcagaagag
     1021 gtaattccat tctcaatggt gatggttcca tttgtcattg cgttaggata tgactcgatc
     1081 gtagcagtaa ccgtgactta tgtcgcatct caagtcggaa atgcgacctc ttggatgagc
     1141 ccgtttagcg tagcggtggc tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca
     1201 ttccgattga ttatgtgggt agttgttact gcattagccg ctggttatat gatgatatat
     1261 gcagaaaaaa tccgtaaaaa accagaaagt tcgttgactt acaaatcaga tgattatttc
     1321 cgttctcata tcaaaaagac atctgatgaa aataaaaaat tcatgcttgg gcataaattg
     1381 atcttattag agatgttagt tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc
     1441 tattacattc cagaaatcgc ttctcaattc tttgtgatgg gattagttgc tggaatcatt
     1501 gctgttcttt tcaaattaga tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct
     1561 ggtgcagctg atttagctgg tacagcaatc gtagtaggta tggccaaagg gattcttctt
     1621 gtcttgggag gatctgatgc atctgttcct tcagctttga atacgatttt gcatgggatc
     1681 gggacggcgt tgaccggtgt tccagctgtc attggtgctt gggcgatgta catcttccaa
     1741 agtttgttca acttggttgt tacatcaaat tcaggacaag cagctttgac tatgccaatc
     1801 atggctccac tggcagattt agtaggtgta tccagacaag tagcagttct tgcttatcag
     1861 cttggttcag gttttatgga cgcttttaca cccgtatcag ccagcttgat tggtgtgcta
     1921 ggtgttgcac ggatcgaatg ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc
     1981 ctgtttgttt taggaactat tttcatcatg attgccattg caataggtct gcagtagtac
     2041 tggagacctg taaataaaaa agtaatccaa acagtagata aggactccaa gaaccgtcat
     2101 tttacggtaa aatcaaagga gtaaagatcc tatcgtttgg attatttttt aagatcctat
     2161 catcagttat acttaaagaa agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg
     2221 tttgcaccag attacttagg aaaaatggat gtactgattg ccggtgggaa gattttggct
     2281 gtagaagaac agctagaagg cggctacgaa ggagtggaag tagaagaact ttcaggtgag
     2341 ggaaaagtac taaccccggg tttcattgac tgtcatttcc atattttagg tggaggagga
     2401 gaaggcggtt atcaaaatcg aacgccagaa gtcacattga gccaattgac tacagctggt
     2461 gtaacgacag ttgtcggttg tttgggaaca gacggagaag gtagagatat gactgcgttg
     2521 atcagcaaag cgaaaggtct ggaagcagaa ggaatctcca cttatgtata cgaaggttcg
     2581 taccgattgc cggtaaaaac agtaacagat tctatcatca aagacttttt gacaattgat
     2641 aaaattatcg gaatcggaga aatcgcagta tccgatcacc gttcttctca gccaagtttt
     2701 gaagagttcg ctcgtgcagt agcagatgct cgtgtaggag gcatgttatc cggaaaagct
     2761 gggatcatca atgtccactt aggcggagga aaaagaaagc tagagctatt gacacgaatc
     2821 gtagaagaaa cagaaattcc gatcacacaa tttttgccta ctcatgcaaa tcgcacacca
     2881 gaattatttg aagcttgcgt ggcatttgcc aaacgtggtg gcacgattga cttcactgct
     2941 agcgaagatc ctgatttttg ggagaagaca gatggtgaag ttcgcttcag caaagcactg
     3001 aaacgattga tcgaggaaga tgtttcttta gataatttca cgatgacctc agatggacaa
     3061 ggaagcttgc cttattttga tgaaaacaat catttcctag ggttaggagt aggaagtgcc
     3121 aaggcattgc tagtagggat caaagaagct gtgcaaaaag aaagtatccc tttggaaatc
     3181 gccttacgag ctataaccag taatccagca cgtatattga aattggataa aaaaggaaaa
     3241 atcgaaattg gtgctgatgc agatctgtgt atcttggata aagaaacgct tgatattgat
     3301 acagttatcg ctaaaggaga aataatggtc caagaaaaag aagtcaaagt atgggggact
     3361 ttcgaaaaaa gtttttaatc aacagctcta actatctttt ttagaaaacg agcgttctgt
     3421 ttgtctgtat ctgttgttga tgttaccctg aaggtagatc aataaacaaa ctaagaggag
     3481 gaagcacaat ggcagattta aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg
     3541 aaggaaaaga agcatacggc aaagcaacag acgacaagag caaagaaaca gaagggaaag
     3601 cacaatcact aggtgctgac atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag
     3661 acaagttttc tcgaaaagac gaaaaaaaat aaaaaccact tattactaat tgaatcaagc
     3721 aaataaaaaa tccgaaccat atctgcgaga cagatatggt tcggattttt tatttgatca
     3781 ttatcctgtt ttttaaactt cgattttttg aaatttttta ttttttagtt caaagacttc
     3841 atcacagctt tttgctactt caatctcatg agtcacgatg ataatacagc gattttgttc
     3901 atgggcgatt tcttggaaaa gtttgacgat atcttgtgaa gttgtttcat ctagatttcc
     3961 agtaggctca tccgctacga ttagttgatg ttcacagcag atagcacgaa cgatagcgac
     4021 tcgctgctgc tgacctcccg aaagtttggt cacctgtttt tttgctaata tttcatcaat
     4081 tcctacacga gccagtcgat ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt
     4141 gatttccatt gctgctatga cgttgtctag agccgataaa taaggaagca agttgtaagc
     4201 ttggaaaata atcgagacat catttttacg ataacttctt aacccgattt tgttcagtga
     4261 acgatcttga tagtatacct ttccttcttt tggtgcatct agtccagaaa taagcgaaag
     4321 aaaagtcgtt tttccagaac cacttgctcc taaaatagcg tacatttttc cagcttcgaa
     4381 attcagttct acatctttat aaagcgcatc ttgcggatct tgataccaaa atcccaattg
     4441 ttctgtccgt aacatcaaat tcctccttta gtttaatagg atttttcttg ggtttaatct
     4501 taatatccct atagaagcta aaaagacaga aaacagactg attccaaatg ctactccggc
     4561 taatagtccc aattgtgcag gtttgacatt gatttccaat tcgctgactt gatcagaaac
     4621 agcaaatgga ttattcgtgg ttccttgttg accatcgcgt tgaccgcctt gttcttgtct
     4681 gttacctggc atttgtccac cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg
     4741 ttggtcaagt agctgctgtc ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac
     4801 tcctaatgca agaatcatac aaatagccac ttcggtaaac aattggagaa tgattttact
     4861 tctggattct cctaaagata gcaagacgcc taactcatgc cgacgctccc gaatggtaat
     4921 cataatgatc agagtaagta tgataatacc ggctacagcc actagtaaaa caacattttt
     4981 agaaaaactt gccacgttgt ttaaaggttc caacattgac tgatacatgg aatcatttga
     5041 ttgtaagcta aatgtatctg tgtcgatcag cttctcagct tctttgagaa actcatccat
     5101 tttatctgga tcagatagag tgtaaatagc agaatctatc gtgttatcgt cagatgtccc
     5161 atttagttca tttgcaaaag tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt
     5221 gaatttcata cccatactgc cgcttgtttc gctggattca taaattcctt tgatcgtcat
     5281 ttcataagtg gtatcttcat cttcagtact cgtgattgtg aaagtatctc cgactgccag
     5341 gtcatttgtt tctgctagag tagaatcaat gactacattg tttgtgcctt tgtcgctgtc
     5401 atctattcct tcaccatccg tgatttttgc tgttccatca gaaaaattac tattttgtgc
     5461 agttgctgac acaccactga tttgaaagtc tgcttgagtc atttgcggat tcccgcccat
     5521 tcctccagca ggttcaccgc ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga
     5581 actagtgatg gcagttattc catcttttgc catagctgag gcggaacttt caaaggaata
     5641 actctttaca ttatctaatt cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag
     5701 agaaaaactg cctggatctg gacgttcttc agtggtagaa ccgcttgaag aagtttgttc
     5761 ctgtttttta aatgaagctt ctcgattggc acttaaagtc actgttgctc caacttcttt
     5821 ttgtgcttga ttcgctgctt cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa
     5881 aataagaatg gctgtgaaca cggcaaacaa taacaatgaa cgtccccatt ttacttttgt
     5941 acttttccat gcacgtttca taaaattcat attttgcatc cctcctagta gatgcatttg
     6001 ataatagaca acctatctta aagctagctt aaatcttatg ctgtttgcac accatcaaca
     6061 tgaggttctg tatatataaa aagtcactat agttattgca aaaaatgtgg taaaatgaaa
     6121 aaagtatgtt tcaaaccgac gaagaagaaa tgaaagagga atagcaaaag aaatcctaga
     6181 tgctacagag ggatttttac gtgcatagtt gttctaacaa ttgctgatac tagcgattta
     6241 gtttatttgt tgtataataa tggggaacag ctgaatactt atataagtat tctggctata
     6301 gcgtttcgca aaagatccat tttgttaatg aagtatatcc aaaataaaat gcccatccaa
     6361 ctgtcagaac gcggatgtgc tggtaatttt cctgctgatt ctccataagt tgagaaatag
     6421 tgattttgca tatgcttcat tggaatggct catatattta gataaagaag aaaatcgaca
     6481 tctttaaact tggatgtcat aagaagaggt gaagtttgtg agtaccatta aaattatccc
     6541 attaggcggc gttcgtgaaa atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat
     6601 ttttatttta gattgtgggc taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt
     6661 aatccctgat tttacgtatt tagaagaaaa cattgatcgt gtagctggtg tctttttaac
     6721 acatggacat gcagatgcga tcggggcact tccttacttg ttgtcaaaaa tttctgtacc
     6781 agtattcggg acagagttga cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc
     6841 agccaaagga tttaaggatt tccatgtggt agatgagcat acagaaatag actttggtga
     6901 aacagtagtg agctttttcc gtaccactca tacgatacca gattcagtgg ggatcaatct
     6961 gaaaacaaaa gaaggtagta tcgtttatac gggagatttt aaatttgacc aaacggctat
     7021 cccaatgtat caaacagatt atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc
     7081 cttactaagt gaatcggcaa atgcagaaaa tcctcagcct gtagcttctg aagcccaaat
     7141 agcagatgaa gtatttgata cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt
     7201 agcaagtaat ttgcaacgag tgcaacaagt attggatgca gcagaaaaag cacaacgaaa
     7261 agttgtcttg actggtcaag atttcgaacg aattattcgc acagcgatgc gtttagaaaa
     7321 acttcaatta ccaagtgaag atctgcttgt tacgttgaaa gacatgaaaa actatgatcc
     7381 agaagaactg atcattttag aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa
     7441 aatggcaaac ggtacacacc gtaatgtcaa gattgaagaa ggagatttgg tttatatcac
     7501 tactacgcca agcatcgcga tggaaaccat cgtggcgaaa acggaagata tcatttatcg
     7561 agctggtgga acagtgaagt tgatctctga gaatatgcgc gtttctggcc atgccaatcc
     7621 taatgacttg caattaatga tcaatttgat gaaaccaaca tattttgtgc cagttcaagg
     7681 agagtatcgt gaattagctg ctcatgcaga tttggctcat gcagtaggga tgccatacaa
     7741 aaatatctat attactggac gcggagacgt gctggaatat aagaacaagc gtatgagtgt
     7801 tgcaggaact acaagtgcag aaaatgtaat gattgatgga ttaggcatag gtgatatcgg
     7861 aaacattgtt ttacgtgatc gaaaaatctt gtcagaagac gggattttcg tagcggttgt
     7921 cactatcagt cggcgagaaa agaaaatcat ttcaaaacct caaattactt cacggggatt
     7981 cgtttacgta aaagctagcc gtgatctgat gaaagaaagc agcaatatga ttgaaacaat
     8041 cgttgagaaa catttagaaa gcaatgattt tgagtggagc aaattaaagc aagacatccg
     8101 agatcaatta ggccgttact tatttgaaca aacaaaacgc cgcccagtga tcttgccagt
     8161 cattatggaa gctactcaac gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc
     8221 tctatagagt gctcgtcttg ctcttttttt gcgaaaaaca aataattttt gaatacagca
     8281 tccctcgctt tataataaac gatagaataa agggagtgaa tcaatgatga gtcaaacaaa
     8341 agtcgaacgt tatttagaag aacaaaagat cagctatcaa ccacttgatt ttagtcatgt
     8401 ggtagaagaa tctttcacag aagaactaca aaaaagaggg attgatccag ctttgatatg
     8461 taaaactctc gtagtgaaag gaaacaaaac tggggtgatc atcgcattga ttcctttgaa
     8521 cgatcactta gattacaaaa aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc
     8581 tggaatggat tttgtattaa tgcatacagg atatccccac ggagcaaaca cccctattgg
     8641 aataaaactc gcacatcctg attattgttt tttagcagac agttctttaa aagaaaaaga
     8701 agaagtaatt gtgtcaagcg gtgaaatcgg gagaagcctt cggataaaag tagccgattt
     8761 agaaaagatc gtccatccta tttacgctga tttaattagt taagggacga gaagagaagc
     8821 taagcagagc agagacctca aacttgagat tatatctgct tggtacttgg tgcatcctgt
     8881 attatctgtt acaattgctt tgtacatact gtactatcaa tccaatgaaa atatatttag
     8941 cagctatatt ttttcattga tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag
     9001 agaaaacgcg aggtgtaaaa tatgaaaatt acgtatcatg gacattcctg tttaacaatt
     9061 gaaatggaaa atggccaacg cgtgatgatt gacccgttta ttacaggaaa tccattgaca
     9121 gacttaactg cagaagaagt aaagacagat tggttgctga ttactcatgg tcatagtgac
     9181 cacataggag atatgattcc tattgcaaaa gaaaatcaag ccacaattat cagtattgtt
     9241 gagattgctg attatgcgaa cagcagagga gtagattcat tcggtatgaa tatcggtgga
     9301 aaacatgcgt ttccattcgg cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat
     9361 gaagtagacg gtatcgttca gtatatgggc gagcctagcg gaattattat tcaagcagaa
     9421 ggaaaaacga tttatcatgc aggggatact gcttatttca gtgatctagg attgcttgca
     9481 gaagaattcg atattgatgt cgctttcttg ccgattggcg ataactatac gatggggcca
     9541 gaagatgcag caaaggctgc gcaaacaatt cgtgccaaga aagtagttcc aatccattac
     9601 aatactttcc ctgtcattga acaagatccc gaatcttttg tagagttact ggaagatagt
     9661 gaaggaaaaa taatgagcgt ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa
     9721 acatgatttg attttagaat atattgaaag tttacctgtt ggcaatcgaa tttctgtaag
     9781 aagcatcgca aaagaattga atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc
     9841 agaaaatgtc ggactagttt caacaattca acgagtaggg accatccgta tcgaacgcaa
     9901 attaaaaaaa catattgaac gtttgacatt tggcgaggtc gtcaggatca ttgaagggga
     9961 cgttttaggc ggggcggcag gattagacaa ggtcttgaat aaattcgtca tcggcgcgat
    10021 gactaaacat gccatggaac gctatattac gccaggatca ctaatgatcg tcggcaaccg
    10081 ggcggaagtc caaaaattag cattggaaga tggcgccgca gtattgatca caggaggatt
    10141 caatacgact gctgagattg caaagctagc tgatgatctt gaaatgccga tcttacgcac
    10201 ctcttatgat acatttacag tcgcaaccat gatcaaccga gcgttaagcg accaattgat
    10261 caaaaaagat atcatgcttg tcagtgatat ctatatgcca gctgaaaaga ctcattattt
    10321 gcagcagaca gatacgattt ctgattatca gcgtttgtca gaagaaacgc agcattcgcg
    10381 ttttccagtc gtcaaccgcc atcatcgttt gatggggatt gttacggcaa aagatgtttt
    10441 agggaaatcg ccaaaccaga tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa
    10501 gaaaacaatg agtattgcct ctgttagcca tcaaatgatc tgggatgggc tagaagtcat
    10561 gccggtcgtt tccgatgact tgacattgga aggcttgatt acccggcaag atgtaatgaa
    10621 agcgatgcag cttgtccagc gacaaccgca gattgcagat acgatttcgg atcagatatc
    10681 tggaaatatc catacgattg atactgaccg tgaaggaaat cgtttggaaa accctaagtt
    10741 caaattcgta gtagcaccac aaatggtcaa tggcgttgga accatttctt ttggcgtact
    10801 tagcgaaatc atttctgatg tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat
    10861 cttgattgaa caagtgaacc tacattattt gcggctgatc caattggaaa gcgaattaga
    10921 cattcgtcca cgtgtattgg aaatcggcag acgttccgct aaactggata tcgaagtgtt
    10981 tattgaaaac accatcgttt ccaaagcaat cgttgtttgt caagtaatgg aacgttctta
    11041 gaaagcagga gtgaaatgac agtacagcaa gaaatagtag atacaataaa aaaatacgat
    11101 aaaatcatta tccacaggca tttgcgccct gatcctgatg cattaggatc acaagtagga
    11161 ttagccgaac tattacggga aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg
    11221 attgaagggc ttcgcttttt agcagaaatg caagaagttg aagatgtctt ctaccaagga
    11281 gcgctagtca tcgtgactga tacagctaat tcacccagaa tcagtgatca aagatatcag
    11341 ttggcagaaa aactgatcaa aatcgaccat catcccaatg atgaccctta tggagatctt
    11401 gtgtgggtaa atacaaaagc aagtagctgt agtgagatga ttgcagaatt tgctttgatg
    11461 tttcctgaat ctttaaagat gaacgttaag gctgcacggt tactttatgc aggaatcgtc
    11521 ggagatacag ggcgcttttt gtatccctcc acgacgtccc gtacattaga gattgcttcg
    11581 atattgagaa attatccatt tgatgcaagt gctttgaaca gagagattga acaaatgccg
    11641 atgaaagtcg ctaaactttc cggttatctt tatcaaaata tccaggtgga tgaaaatggt
    11701 gcaggaagaa taatccttcc gcaagaaatt cttgataaat atggaatcga tgattcagag
    11761 acagcttcga tcgtttcact tcctggagtg attgacgaag tgctggcttg ggggattttt
    11821 gttcagcagc cttcagggta ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat
    11881 gaattggcta aacgccatca tggaggaggc catccattag caagtggtgc gaatgcaaaa
    11941 gatttagcag aagtagagga aatctataaa gagatccaag atatatgcca atcgtataag
    12001 agcagaaaaa cagaatgata cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa
    12061 tgtatctttt caagatcttc tttttccgtt acaatagtgc tcactaaaga ttttaaagga
    12121 gaaagaagat gacattacca aattgcccag aatgtggctc agagtacaca tatgaagatc
    12181 gaggcttgtt gatttgccca gaatgcggga acgaatggaa caaagaagaa caagaaactg
    12241 tcatagaaga cggattagtg gtattagatg caaatggaaa ccgtctgtta gagggcgata
    12301 gcgtgacagt gattaaagac ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa
    12361 caaaagtcaa gaacatccgt ttagtagaag gcgatcataa tatcgattgc aaggtagaag
    12421 gatttggacc gatgaaactg aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg
    12481 tcttttcaca ttcttttcct actactaatg ataaaataaa gtaaacgaaa aggagtgaaa
    12541 cgaaaatgta tggttatgga atgtatggtt ttttagatcc gacatttctt ttggtcattc
    12601 ttggattagt tatttccgga attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg
    12661 atcaagtgag aagcaaaaac aatgtgactg gtacacaagc agcacaatat atattacaaa
    12721 gtcaacagat aaataatgtc ggtgttcaac aaattgccgg agatctcaca gataactaca
    12781 attcaggtaa taagatgttg agtttgtctc aagcaactgc acagtctact tcagttgctg
    12841 cgataggtgt agcagctcat gaatgcggac atgcggtaca agatgcttcg ggttatgtcc
    12901 cattgaagat tcgtgcagca cttgttccag cagctaactt tggttcgatg atttcgttcc
    12961 ccttgattat ggtaggtgta ttgcttagct ggaactctac gttaatcaat atcgggattt
    13021 tcgctttttc tttagcatta cttttccaat tggtgacatt accagtcgaa tttaacgcgt
    13081 ctcggcgagc aatccaaatt ttgagtgaag gcggactact gacggaagaa gaagtaccga
    13141 tggcaagaca cgtattattt gcagcagcac tgacttatgt tgcagcagcg ctttcgacat
    13201 tcctgcagct gttgcgcttg atcttattgt tcggcggcaa ccgaagagat taaaataaag
    13261 aagttgtgaa atcagcgctt tgatctgagc taataccgag agttagctga ttgaatacca
    13321 tttatttgga ataagggctc tttgtcaata aggactgatg ggttgtacaa aatcaaatct
    13381 gggtcagaat gaacctcatt ctggctcag
//
LOCUS       DACDMM010000068        13354 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDMM010000068 DACDMM010000000
VERSION     DACDMM010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.068"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            189..956
                     /locus_tag="IWD16_001547"
     CDS             189..956
                     /locus_tag="IWD16_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9088610.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            946..2988
                     /locus_tag="IWD16_001548"
     CDS             946..2988
                     /locus_tag="IWD16_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9088611.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            3269..3418
                     /locus_tag="IWD16_001549"
     CDS             3269..3418
                     /locus_tag="IWD16_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP9088612.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            3429..4943
                     /gene="dltA"
                     /locus_tag="IWD16_001550"
     CDS             3429..4943
                     /gene="dltA"
                     /locus_tag="IWD16_001550"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP9088613.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            4940..6145
                     /gene="dltB"
                     /locus_tag="IWD16_001551"
     CDS             4940..6145
                     /gene="dltB"
                     /locus_tag="IWD16_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP9088614.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            6190..6423
                     /gene="dltC"
                     /locus_tag="IWD16_001552"
     CDS             6190..6423
                     /gene="dltC"
                     /locus_tag="IWD16_001552"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP9088615.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            6426..7694
                     /gene="dltD"
                     /locus_tag="IWD16_001553"
     CDS             6426..7694
                     /gene="dltD"
                     /locus_tag="IWD16_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9088616.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            7835..8917
                     /locus_tag="IWD16_001554"
     CDS             7835..8917
                     /locus_tag="IWD16_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP9088617.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            8917..9720
                     /locus_tag="IWD16_001555"
     CDS             8917..9720
                     /locus_tag="IWD16_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9088618.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            9790..10530
                     /locus_tag="IWD16_001556"
     CDS             9790..10530
                     /locus_tag="IWD16_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9088619.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            10541..11545
                     /locus_tag="IWD16_001557"
     CDS             10541..11545
                     /locus_tag="IWD16_001557"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP9088620.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(11590..13353)
                     /locus_tag="IWD16_001558"
     CDS             complement(11590..13353)
                     /locus_tag="IWD16_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088621.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT         4293 a         2190 c         2701 g         4170 t
ORIGIN      
        1 gtgaaaaaag gtgaaaactt gtagctggtg gatttgtttt ttgcatattt aattttgctg
       61 aataaaataa ttgaataaaa ttttttattc agtccaagta aaagtgacaa aaacataaga
      121 gcaaattatt cgtagatatg ctactatgca aataaataga aatgaaaaat gaaagaggag
      181 gagaagaatt ggaaaagatg atcgaagtga aacatctaag taaaagctac ggcagcaaac
      241 tttctcccgt caaagcctta gataatatct cattcacagt agaagaaggc gagtttgttg
      301 ggattatggg cccaagtggt gcaggaaaga caacgctgat gaatatcttg tccacgatca
      361 atttgcctac gatgggcagt gtctcgatcc aaggaaaaga aatcacaaaa atgaaaaaca
      421 gtgagctcag tgactttcgt cgaaaaaaac taggattcat ttttcaagag ttcaatttga
      481 tcgataccct aaatgcaaaa gacaatatcc tattgcccct agcagttgaa cggatgacta
      541 aagaggaaat ggaaaagaga gtaagacatg tagctcaatt gttgaatata gaagaactgc
      601 tcaaacggta tcccgatgaa ctttctgtcg gacagagaca acgaattgca gctgctagag
      661 cacttgttgt tcagccacaa gtgatttttg cagatgaacc aactggctct ttggactcga
      721 agtcggctac agaattgttg aactacttga agaccatgaa tcaaaaagaa aaagcaacga
      781 tactgttagt cacccatgat ccatatacag caagctactg cgatagaatt ttatttatta
      841 aagatggtgt gattttttct gaagttgtca gaagagggac aagaagagag ttctttgaga
      901 aagtaataga catgcaggcg acgattggcg gaggaggaaa aatgaatgct gtataaacta
      961 gccgttaaaa gtttttttcg gcagtcacga ggctatcttg tttatttttt cagtttgact
     1021 ttatccgtaa tgatctatta ttccttcagc gcaatgacct acgatcaatc tttagtcaga
     1081 agagcaagtc aagatacttc gatagatggt ggattgagtt ttggtggatt tatcattact
     1141 gttgttttgc tttttttcgt attcagtgcc aaccgatttt tcttgaatag aaggcaaaag
     1201 gaaataggca tctatcagct ttttggtatg aataaactgc agatatcagg tatatatgtg
     1261 ttagaaatca tgataattgg attgttcgcc tgtatttctg ggatattact cggtattatt
     1321 ttttccaaac tcttttcaat gattttggtt cgaatgatgg atatggatct tactagtcca
     1381 ttttttatct ccattccatc agtagtagat actttatttg cttttttcat catcctttta
     1441 gcagtatctg tatattcaat atggaaaata tggcgctatc caatgattcg aagttttgga
     1501 gaaaaagaac aagcagatag ttcaacaatg cgcttcagga caaggcaccg attactcgca
     1561 gtcatcgggt tactactttt aacgagcgga tattttggag ctagtcattt tagggaaatc
     1621 acttcatgga tgatcagtta tcattatctt gatacattag ttttcttttt tcctttttta
     1681 atttttttcg tttgtgtggt cggtacttat ttgtttttct gttatacatt tcggttattt
     1741 ttgatcatat tgagtaaatg gaagatttat tactatggta tcaatttttt gataatgggt
     1801 aacacgcaag ttcatttgct caaaagttgg cgtacaaatt ctctgattac agtcattctc
     1861 ggaatttctt taatgacaat tggcggcacg gctagtatct ctactatttt atcctaccag
     1921 gagaaagttt cctccccgat ggactatcag tttgatgttg agacagagaa aaaaatcaag
     1981 ccgattctag cagaggaagg gcagaagata actaaagaaa tcaccttgca ttataaagtc
     2041 attgggagta tctatagtca aaatctcgaa gaacctatgt ttcaaactgc taatctgatc
     2101 acggaaacac aatatcaagc cttcagaaaa gttaatccag atttgccgaa catttctttg
     2161 aaatcacctg gtcatactgt tttgcttgat caaatgcaaa ctctttttcg gaatatttca
     2221 ttatatggat ctagtattgg actgccagat aatcaagaat tgaagattca acaaatgctt
     2281 ccaagtgtat taggtgacga aacaatggta tatctaagtc ctgtactgat tgtagatgat
     2341 accgtttttg aaaaagcaaa aggtgtggat tatcaagtag tcaatgtaga tgtatcaggt
     2401 ggaaataatg aaaaaataga tgaacgactg agcaatgacc tgtcgataaa gtggctcaat
     2461 gctgtttatt attcttatga tatcgtaaat ggaagtctac aaggagaaat tagcacaaag
     2521 aaacttccgg atgaccaaat gaaggaagca caatttagcc aagaaagctc tcgtttgaat
     2581 ttctcttcca gctattcgaa attgcgtgca gttaatcggc gaatcggtat caatattttt
     2641 gtcacactat ttgtcgggat gatcgtgatt attgctactg gtagtatttt gacagtccgg
     2701 caattagcag aagcagagga agaaaaagaa aattatcacc tattgactaa gctggggatt
     2761 ccacaaagaa ggatcagacg gatgatcttc gagcaaaacg cacttacctt ctttccaccc
     2821 atgatattag ggatcactca cgcagtattt gcaatcaatg tcttcactca gtatataaaa
     2881 actgccgatt actggttggc ttatcttgtg agtggcttat tgatccttat ctatcttttg
     2941 ctttatgtca ttacgagtca gttatattgt cggattatcg aggaatgata tacggaataa
     3001 gacaagataa taaatagaac taaattttga ataccattta tttggaataa gaggttgtga
     3061 cacgattttt gtcacaacct ctttattttt tatatttttt aaaagtacga gaaattataa
     3121 agaaaaaagc agtgatttga tgtgctattt ttataaaagc tatgtgacaa aactgttagt
     3181 aaaaaaacgg tttctgtaag ctagacacat gtttcttttt gtcaaaatta gttacacttt
     3241 gtgtgaggtt atatgaggag ggaatccaat gaacaaatgg cgttctaatc caaaattgaa
     3301 ttattgggga aaattcatca ttcgaacaat tttttatagt gcagtactgc tgttcctgat
     3361 ttatctgtat cactataaaa atattcaagg aggaactttt atctataacg aattttagga
     3421 ggaagagtat ggaaatcaaa acgattattg aagcaatcga tgattgggga ataaatgaac
     3481 ctgatcgagt tgcttatcag gcggatgaga cgcatacttt tggtgaattg aaacaagcat
     3541 cagacgcact tgcttattat ttagaaaaaa aggtagaagg cgatggacca attgtcgttt
     3601 ttggcaattt ggagtttgaa atgattgtct catttctagg ggttgtcaaa tctggccatg
     3661 cctatttgcc gattgaagag catacaccca aggagcgtat cctatcgatc tttcgggtgg
     3721 ctaaaccatc tatggtcatc agtatcgggg actggataga agatattcct tcggttcctg
     3781 tcataacaaa aaaagaattt caagaaatca agaagatacc agtaggattt tatcccagaa
     3841 atagcgtaaa aggtacagaa aatttttata ttatttttac ttctggtact actggagaac
     3901 caaaaggcgt acaaatcagt catgataatc tagtcagctt tgttcaatgg acgatggaag
     3961 actttgggat caagcctggt atgcatttct tagcccaagc gccattttct tttgatttat
     4021 cggtattttc tatttatcct gcattagttt ctggcggaat gctgaagccg ttaaataaag
     4081 cagttgttca agactttcgt caattattcg cgacattgcc agccttgaag ttgaatgtgt
     4141 gggtttctac tccatcattt atggatattt gcttgatgga acctacattc aatggagaaa
     4201 atgtgccgga attggacatg tttttatttt gcggagaaga attgacgaaa aaaactgcag
     4261 aaagtctact tgatcgattc ccaaatgcac ggatatataa tacttacggt ccaacagaag
     4321 caactgtagc tatttcaagt attcagattg accagcatgt actagatagt tatgaccgac
     4381 taccgattgg ctacgtaaaa aaagacacga aagtttctat tgtccaagaa ggtaaacctg
     4441 tttcaaaagg agaaacgggt gaaatcatta ttgcaggacc aagcgtatca aaaggttacc
     4501 tgaacaatcc cgaaaagaca gctgctgcgt tcttttgtca aaatgactta gcaagttacc
     4561 atacagggga tgcaggtaga ctagatgaat atgggctttt attttatgaa ggacgtatgg
     4621 atcagcaggt caaattacat ggataccgga ttgaactcgg ggatatcgaa cattatctgc
     4681 tccaagacaa ccgaatcaaa caagctgtcg tcgtaccaaa ataccaagga acaaaagtac
     4741 agcagctggt tgcttttgtc gtattagaaa acaaaacgaa acagccggat tttcagttga
     4801 ctaaatctat caaagaacaa ttactagaag ttgtaatgga ttatatgatt cctcagaagt
     4861 tccattatgt cgatctgctg cctcagacag ttaatggaaa aatcgatcga aaaaaattaa
     4921 cagctgaggt gaatcctgaa tgatgttacc tcatatgatc ccttatgcag agccgtttta
     4981 tttttttctt ttagccattg cgcttgtacc gatcgttcta tctttattga tatgggaaaa
     5041 aagattacca gtttatcaat cagcagtgac acttttcttt ttatttgtca gctttggagg
     5101 taattattgg caacagggag cggcgctgat tgggtatgtt ttgtggcaga cgatacttgt
     5161 atggggctat tttgtttatc ggcaaaggaa aaatcagaca ggatggtttt ttgccgccgt
     5221 tattttagcg ataattccac tgtttttaac gaaagtgact cctttttttg aacatggaaa
     5281 agcatcgtta cttggttttt taggaatctc ttatttaacc tttaaatctg tacaaatgat
     5341 catggaaata agagatggca tgatcaaaac atataagcca tttcgttata tccagtttct
     5401 gctgttcttt ccaacaatct cttctggacc aatcgatcgc tataggagat ttgaaaaaga
     5461 tcagctccat ccgcctgttc gtgataaata tgttgatctt ttgggaaaag gcgtccaaaa
     5521 tatttttgtc ggttttttat ataaattcat tatcggttat tattttggtc aagtactgct
     5581 tcctgtagtg ggacgcattg ccttacagca tggtggtatt tcatgggcat tagtaggtta
     5641 tatgtacgtg tacagtatgt atttgttttt tgattttgct ggctatagct tatttgcagt
     5701 cggtacaagc tacatgatgg gatatcagac accaatcaat ttcaataaac cttttttatc
     5761 atggaatatc aaagaattct ggaatcgttg gcatatgacg ctgtcctttt ggttccgtga
     5821 ttatgtatat atgcgattga tgttcttcat gatgaagaaa aaatttttta aaagcaagat
     5881 cgtaatgtca aatatcggtt attttgcttt gtttcttcta atgggtatat ggcatggatt
     5941 gacgtggtac tacatcgtat atggactgta tcatgcttgt ctgatctgtc tgacagatgc
     6001 atggatccga tttaagaaga agcataaaga gcagttgcct tctaacaaat ttacgcatgg
     6061 cttagccgta tttttgacat ttcaagcagt atgttttagc ttcctgattt tttcaggatt
     6121 tttagataaa ctttttttta gctaacgaat agtgacatat taatttatat aacaaaggag
     6181 aaaaggaaaa tggaagaaca aattttatca atcttagaag aaataacagg aacagatgaa
     6241 gtaagaaaaa atcgtgatat tgatctattt gatgaagggt tgcttgattc tttagcatct
     6301 gttcaattat tagtcgagct agacggagag ctggatatcc aagtccctgt atctgaattc
     6361 gagcgagagg attggagcac accgaataaa atcattcaac aagcaactgc attgaagggt
     6421 tgagtatgaa aaagaaactt tttgctattt tcggcccggt tgttttagct gttggcttac
     6481 tgtttgtgtt ttttacctcc tcctatcaag tagatatctc taatccagaa acgattagac
     6541 gtgcctcttc ttccatgtca gtgaacgtgt tgaaaggaga tgcgataaaa aattatgcat
     6601 tatctgaaaa acaatatatt cctttttttg gctcaagtga attaagcaga atcagtccat
     6661 ttcatccgtc tgttctggca gaaaaatacc agcgaaacta tcgtccattt ttgctgggag
     6721 cacccgggac acaatcgtta agccaatata tgatgatgcg ttctgcagga gatgctatga
     6781 aaaacaagaa agtcgtcttt attatttctc ctcagtggtt cgttaaaaat ggtgtaaaaa
     6841 ctgattactt taatacgtat tattctgaac ttcaaacata cgactggttg ttttcaatga
     6901 agaaagtaac gcctgctgat cgctatttag ctagaagatt attaactttc tcaaaagtaa
     6961 aagagaatga tacgttgacg gcgattctgc aaacgatcaa aaaagggaag ctacctttac
     7021 ctgaatcttt gaatcagctg cgcagccaat ggaacatgct gaagagagag gatgaagtat
     7081 tcagcaatat tgggttgaaa gatcgacagc aaaaaatcga tcatgagtca aaacgattac
     7141 caaaacaata ccaagaaaca gaattatcca ttttagcaaa tcaaattgga gaacgagaaa
     7201 cgacgaataa tccattcggg ttgaaaaacg atttttatac gcatcgtatc cgagcccacg
     7261 agccagagct caaacagtca caaaaaaatt gggattatcg tttttcgcca gaattttctg
     7321 attttcagct ggtcttggat caattagcaa aaaaccataa cgaagtattg ttcatcatcc
     7381 caccggttaa cgagaaatgg agcgattata ccggactatc tcaagaaatg ttacaaggat
     7441 ttgccaaaaa gatcaaattt caattaaata gccaaggatt caatcgaata gctgattttg
     7501 tcaatcaagc gggtaccaac tattttatgg aagacacgat ccatctaggc tggaaaggct
     7561 ggttggctgc agatcaacag atcagaccgt ttttagaaga aaatcatatt actgctagta
     7621 agtatcatct tgacgatgct ttttttagca aatcttggca acatcaaata ccagataaac
     7681 tacaattaaa ataaagaaaa caggcgagtg ttgctcgctt gttttcttta tttttgaata
     7741 ttctgaataa aagataaaaa taccaagttt gcctgaacaa aaatcataag caatggtata
     7801 atcacaatat gaatatagaa atgggggaaa acatttggat acaaaagaag aacagctact
     7861 tattgattta acttctgcaa aaggagtacc tggaaatgaa gaagaggtaa gagaagtatt
     7921 cagagaatat gccaaaccat tcgctgatga tattttttat gacggtttag gcagtgtgat
     7981 tgccaaacat ggtgctggcg gaggccccaa ggtctttatt tctggacata tggacgaagt
     8041 tggttttatg gtaacaaaaa taaccgaaaa aggattcttg gaattccaaa ctcttggagg
     8101 ttggtggggg caagtcatgc ttgctcagca agttgaaatc aaaacacgtg aagggaaaat
     8161 cgtccacggt gtgattggtt ccaaaccacc acatgtttta acacctcaag taagaaataa
     8221 accttatgaa ataaaggata tgttcattga tattggcgca tcaagccaag aagaagccaa
     8281 agagtggggc atacgtcctg gagatatggt cactccatat attgaataca aacgaatgaa
     8341 tggcagtaaa tatttactgg caaaagcatg ggataatcgt atcgggacag ctgtttctct
     8401 gcgagtatta gagaatctat ctaaagaagc acacccaaat gttttattcg caggaagcga
     8461 tgtgcaagaa gaagtaggct tgcgaggtgc tcgaacaagt acacaccttg taaatcccga
     8521 tattgctttt gctttagata ctggtacagc aggagacact ccaggaatga caccaaaaga
     8581 agcagattcg attttaggaa aaggcccaca gattttgatt tttgatgcat ctatgatccc
     8641 gcataaaaaa ttattgaatt ttgttattga tgtagcagag gaaatggata ttccattcca
     8701 atatacagtg atttctggcg gaggaactga tgcaggacaa atgcatttga caagagatgg
     8761 tgttccgtct gtagccatta ccgttcctgt tcgttatctg cattcccata catcagtgat
     8821 tcatgaagat gattatttga atacagtgaa actagtcaca gaattagtga aacggcttga
     8881 tagtgaaaca gttgaaaaaa ttcggagtta tgaataatga ataatagaat acctatttct
     8941 gaatatcgga aaaaagcacg agtttctttg gaaaatcaat ggggaatcaa tacttggctt
     9001 gttttcttat ctataatatt tacgagcgtc atccagaact tttttgacgg tttcagtcct
     9061 tttccggaag aaagcatgca gcagcactta ttgagttttc tagtcgataa tttactttta
     9121 ttcgcattca actatgcatt gttttacatt gcgctagtag ttgtacgggg tggcagacca
     9181 aaaagtaacc ttttattttc agttttccaa agaaagtatt ttggttcgat acttggcatc
     9241 aatttgatca attcagtcat caactggcta ctcaacagtt taatctttct gcccatgttt
     9301 ttaatcagcg gagtgagtac ttatatgcag ctgcttttca gttcaaatgc ggtacctacg
     9361 ggtgtcatgc cagaagattt tcttgatatc agctttgttt tatcgatgat agggatgatt
     9421 tttgtttcag cactcatcat gagtattgtt ggcggtttat tccaatttgc tgcatggaca
     9481 aagttcgact atcctgattt gtcggttatt caatgtttga aatatggctg gtttttattg
     9541 aaagatagaa ttggtacgta tattttgttg cagctgtctt ttatcggctg gtatttgcta
     9601 ggagcaatcg ctttgttcat cggtcttttg tgggtcattg tttatgtgaa tgtgtcagtt
     9661 gcagaatttt atgaacaagc acggattgaa aaaggaggtc cagacgccta ttttacttag
     9721 catgtaagca gtttattcat caaaaaaata acttttgtgg taggataaat ctttgagggg
     9781 gaatgtacga tgaaattaac tgttttagga tgcttaggag cgtatcctta taaaaatcaa
     9841 gggacaacag gttatttgct tcaatctgac gggttcaatt tgttgatcga tgcaggtagt
     9901 gctacgctga tcaaattaca agaacacctt gatccacttg atctagatgc tgtgatcctt
     9961 agccattatc atcatgatca tattgctgac cttggtgtat tacagtatta ctggcaactt
    10021 catcctaaga gaaatccgga gacattgctt tcaatttatg gacacacaaa agattccatt
    10081 cactttgaag aattgacgtt ggaaaatgtc tcaaaaggac atccttattt tgaagctgaa
    10141 gaattaaaaa ttggtccttt tctgattact tttatggaaa caatccatcc agtagtatgt
    10201 tacgccatgc gtatcgtaga agaaacaacg gggaaagtat tcgtatttac aggtgattca
    10261 ggttatttgg aaagtttcgc tgattttgcg aaagatgctg atttgtttct agcagatacc
    10321 tatctttttg aaggaaatga acatcatcat gcccatttca cttcaaaaga agctggagaa
    10381 cttgcgaaat cagctaacgt aaagaaactt gtacttacac acctacctca gtttggtgat
    10441 ttagaacaat tgaaaaatga agcgcaaaag gcagcaggca aagggattga tatagaactt
    10501 gctgcagttg acaaagtgtt taacatttag gaggaagaac atgatttttg taccaaatga
    10561 aaaccacgac ccaagaatca atttagcaat tgaacaatat ttgctgcaag aaatgccttt
    10621 agatgaacct attttgctct tttatatcaa tgaaccttca atcatcatcg gcagaaacca
    10681 aaatacgctt gaagaaatca atcgagatta tgtggaagaa aatggcattc atgttgtccg
    10741 tcgcttaagt ggaggcggag ctgtctacca tgattttgga aacctgaact ttagttttat
    10801 catgcctgat gatggggatt cattccgtga ctttgcgaaa gtaacacagc caatcatcca
    10861 agcacttcat gaattgggtg ttgctggggc ggaattgaaa ggccgaaatg atctagtcat
    10921 tgatggcatg aaattttctg gcaacgcgat gtatgctacc aacggtcgga tgtttgctca
    10981 tggaacaatt atgttcgact ctgatatcaa tgaagtagtc aatgcattaa aagtaaaaaa
    11041 agacaaaatc gaatcaaaag gaatcaaatc gatccgttct cgtgtaacga atatcaaacc
    11101 atttcttcca gaagaaaagc aaaaaatgac aacagaagaa tttcgtcaag aaatcttgtt
    11161 gaaaattttc ggcgtagagt cagtagaaga agtaaaaacg tacgaattga ctgatgaaga
    11221 ttggaaaaaa atcaatcgaa tctctgagga atattatcga aattgggatt ggaattacgg
    11281 gaagtctcca gatttcaatt tctctcgcca aaaacgtttt tctatcggtt cgattgaagt
    11341 ccatttgaat gtctcagaag gaatgatcaa agaagcaaaa atctttggcg atttcttcgg
    11401 actaggagat atctctgacg tggaaaaaaa attggttgga cagaaatatg ataaagcatc
    11461 tctatcaaaa atagtcagcc aaattgatgt gaagaaatat tttggtgcga tagaaccaga
    11521 agaattgctt accttacttt attgaaaata aaaaatagcg gaatccacat atatggattc
    11581 cgctattttt tatacaggat tcgggtaatt tcgagagagt tgaacagtga tccgatcaat
    11641 gaaagtatct ttttcctgcg ttagtgttcc tgtgtaacga tccgctacgt ctttcatcat
    11701 cattaagtca aaacgttcct gatcatctaa ttcttctgtg atgcctcgac taaataggtt
    11761 tgtatcgtaa tgtgcactat gttcgcaaca aatgacgaaa tgttcctctt gttcgtgtat
    11821 cgacaaataa atttttcgat acagtggatt ttcgatggca tgagtttctc taaaggaatg
    11881 ctctaaagcg tggatcaaaa gttgagccag atcgtcatcg ttgataggaa gttcttcggg
    11941 aagatctgct tggaaggtta tctgaatatt ccgtttggca gctaacttct ggtaacgcgc
    12001 taagataaga tttgtcacct ggtggttgca aaaagaacac agcatttcga aattttttgc
    12061 ttcttctaat atcttttcca catagttttt agcttttgtt tggtccgtat gcattaaatc
    12121 ttgcagattc tccatctctt ggacaaattc caggcgaagt gtatttactt cttggatatg
    12181 ctgtcgaagc tgctcataat gttgctccaa caaatcattt ttgattttta agaaatctac
    12241 ttgtctccga tctttgtcgg tatttaagat gtgatcgatt agattgtagc tgataataac
    12301 gattagaatg ataatatata aaatcgtaga ccaattgatc gttgtttgat agatacctaa
    12361 tgccgtttgg atgtaaatca agcaatggat aatggctacc aagacgatcc aaggtgtaca
    12421 agctacataa aaacggttat tcttatatgc ttcgccaatg catgcaagtg aagtgactaa
    12481 agtgcttata acactagcaa tattagcaat cggcattaat tcaggaagtt ctgcaggagt
    12541 aaaagttgca taaagcagaa cgccgataaa aatcaaaagt tggaacgtac aaaaaattcc
    12601 gtaccatttt ttatagtaaa acattcggct ccaatagtag cagatgatga aagctgacgt
    12661 taagataata aataaatgag ataaaaaaga atgaacgaat ggttcaaaaa gaataccact
    12721 taaaatatct tctgatacag cttcaaatcc taatgccaaa gcaaagcaac ttaagagaat
    12781 aagtgagtat tcaagacgtt tttgtttata catcaagtag atcgtataga aaaaggtccc
    12841 tatagcgata aaaacagcga ttatcatcgt caatacttgc ggcactgatt gtgaataaat
    12901 aaaactaatc aatggtccag ttgttcctag ataaactttc ggtggcagtc cagcatagtt
    12961 ctgataagga gatgagactg taagggtcaa tttttttccg atataatttg ctggtaaatc
    13021 tatcacagcc aatgagagtc cgggattgct ttcttcctga taatctttcc gttggtataa
    13081 aatcttttcg cctagatata cagtaatcca ttgatggttt cctttgatta gtaaatttgc
    13141 ttcgtccaac ggtttttcaa gtgtcgtttc cataatgaat gtttcgtctt tatccacggt
    13201 aggaatcgta cgagtgtact tgctgtcaaa cttaaaatca ggatcatctt ttgtatagaa
    13261 aacccacgaa tcttttattt ctttcatttg attgctgtcc gcaggagaaa aaataaaata
    13321 aaagaaataa gcaattcctg ttaaaaataa caat
//
LOCUS       DACDMM010000069        13339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDMM010000069 DACDMM010000000
VERSION     DACDMM010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.069"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            10..156
                     /locus_tag="IWD16_001559"
     CDS             10..156
                     /locus_tag="IWD16_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP9088622.1"
                     /translation="MLVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEENRKKAMRK
                     RVSR"
     gene            complement(218..2029)
                     /locus_tag="IWD16_001560"
     CDS             complement(218..2029)
                     /locus_tag="IWD16_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP9088623.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2026..2391)
                     /locus_tag="IWD16_001561"
     CDS             complement(2026..2391)
                     /locus_tag="IWD16_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP9088624.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2554..2949)
                     /locus_tag="IWD16_001562"
     CDS             complement(2554..2949)
                     /locus_tag="IWD16_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9088625.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(2967..3929)
                     /locus_tag="IWD16_001563"
     CDS             complement(2967..3929)
                     /locus_tag="IWD16_001563"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP9088626.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(3929..4141)
                     /locus_tag="IWD16_001564"
     CDS             complement(3929..4141)
                     /locus_tag="IWD16_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP9088627.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4162..4860)
                     /locus_tag="IWD16_001565"
     CDS             complement(4162..4860)
                     /locus_tag="IWD16_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9088628.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4880..5422)
                     /locus_tag="IWD16_001566"
     CDS             complement(4880..5422)
                     /locus_tag="IWD16_001566"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP9088629.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5554..6558)
                     /locus_tag="IWD16_001567"
     CDS             complement(5554..6558)
                     /locus_tag="IWD16_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9088630.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6555..7544)
                     /locus_tag="IWD16_001568"
     CDS             complement(6555..7544)
                     /locus_tag="IWD16_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9088631.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7541..8347)
                     /locus_tag="IWD16_001569"
     CDS             complement(7541..8347)
                     /locus_tag="IWD16_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9088632.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8513..9469
                     /locus_tag="IWD16_001570"
     CDS             8513..9469
                     /locus_tag="IWD16_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9088633.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9546..10064)
                     /locus_tag="IWD16_001571"
     CDS             complement(9546..10064)
                     /locus_tag="IWD16_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9088634.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10152..10301)
                     /gene="rpmG"
                     /locus_tag="IWD16_001572"
     CDS             complement(10152..10301)
                     /gene="rpmG"
                     /locus_tag="IWD16_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9088635.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10529..10975
                     /locus_tag="IWD16_001573"
     CDS             10529..10975
                     /locus_tag="IWD16_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9088636.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11170..13065)
                     /gene="asnB"
                     /locus_tag="IWD16_001574"
     CDS             complement(11170..13065)
                     /gene="asnB"
                     /locus_tag="IWD16_001574"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9088637.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4150 a         2832 c         2276 g         4081 t
ORIGIN      
        1 gtatctggaa tattagtagg aagtctggca acagccgctg ccgttgctgg tttggttgct
       61 tctgtgaaaa aaacagtgat cgatccaatt gacgaaaaag aagcaatgat cgaagaaaac
      121 cgtaaaaaag caatgcgtaa acgcgtttct cgttaataat tatttaaaaa tacgcatgaa
      181 gactttctaa aaaacagata agtcatcatg cgtattttta tttgtcagtc aatataagtt
      241 gtgctatttt atctgcctgt ctcagattcc atggatctgt ataaggtcta gctactgctg
      301 cagttagcca atccacacct tcaacaggta ttccatagca ataaaagtca gactggattt
      361 cccctgaagc atcaatcacg cgattcgtac ctcgctcaac atctatagct tctgaatgat
      421 aacttcctgt attatctgaa aatgaaaaag ggcgaacaag tttgttttcc ttcaaatttt
      481 ttatgacagg attacttgaa cgagaaaagc ttgtttgtgg caatcgagct tcaatcaaat
      541 agcgactgct gatttcttta tcttgtagag aaataacata gcggttgtgc ttttgctgaa
      601 tatcaaatgg cggttcgatc aaatgaaaaa tacccgcatc tatcagtgct gctaattctt
      661 tcgtccgaat gattggcgga ccgatcgtta aaaagctatt tagatgagta aaccatcccc
      721 atagtatttc tttgtattcc ttagcagaaa aaacgtgcca ttcaatagcc tgccggattg
      781 gatcacgcca gtcttttaat gcatcaaatg ttgaagcaaa tgctcctgtg caattgcctt
      841 tttccgcttc ttttatctgc ccttcaataa atattcggct agcttctgaa aagggctcat
      901 tctgataggc aagaggagtt tctaaatacg accaatccca tctataaggg attagtgctg
      961 gatgctttcc gagacactcc tctgaagaga gggagagaaa atcatgctca aaatttttct
     1021 ttggaatcgg caagtgatat tcttcgatga ttttcttgta ataaaacaac tcaacgtctt
     1081 ttttcagata gtcaaaaaat gtttcgccag tcaaaaatcc cgactgatgc cattgctcca
     1141 ggttttcttt tgttagtaac cgtggccatg ccattgctcc gccttgcttt tgatttcttc
     1201 ctcgagggta ataagggagc ccccttcttg atccactgta tacaatcggt tccttgcctg
     1261 atggatgata gaccagtttt cccttttcgt tctcgaagcg ccctcctctt gatactgtaa
     1321 acaatccgac ataatcaaaa aaacttaagc ctagacctct taaaaagaca ttttctttag
     1381 gcggtatctt cgaaagatct acatctgcag gattagaagg aagctgatac agtaaaacat
     1441 ttttctttgc gtagtctacc cacactcttt cttcttctgt ctcatcattt tcccagtgac
     1501 cgcttgctaa aacaagctga tcgatagaat agctttgctg atctgtctgt aaaagataac
     1561 catcttttct tttgcttact ttttttacta aagaataaac aaactgaata gaagctggaa
     1621 attcttcttg caactttgag aaaaaccagc gttgatagag tccgtataaa catcgagtag
     1681 attgctgatc tttttccaga cgattgattt cttctataat tagttgctta tttttggtta
     1741 ccgtctgttc gatataatct gtagcaggtc ctttactcca ttgaaacaag ttgggtccct
     1801 gtgttaccaa tcctccactg gacaatgttt catctgtaaa aagcgtcact tgttgagaaa
     1861 cagaattcat taataactcc ggtggctgat tcaaccgcca aactcgacct ccaggaccat
     1921 tgggatcgaa aagaaaaagt tcaagagaat gtctgtcctg attattatgg agaatccttt
     1981 ctgctacact taatcctcta ggtccggcac caatgatccc tattttcatt tgcatgcctc
     2041 catttcgata aattttttca ataaaacagc atggttctga tgttcatttt ttgcaccata
     2101 aacaaaagta atgactggat gttctttcat aagttgactg agtttaaaaa tagcttgctt
     2161 cttttcttct tcgaaaatca attcttgctg gtatttcttt gcaaatgaag caaatcgttc
     2221 tggtacatgg tcaaaagttt tgcgtgcttc attactaggt gcaatttctt tcaaccacag
     2281 atctaactgc gctttttctt ttgaaattcc tcgtggccag aatctatcca cgagtattct
     2341 atagccatcc tctttttcag catcaaggta aacacgtttt aatttgatca tagttgtttc
     2401 cttctttcta ccttttcgct tagcttatat atgtagataa tagcacaaaa taaaaaaaat
     2461 gaacggcaca actatccatt gcgccgttca tgtgatcttg gtcaaaatct aatgattgat
     2521 tcggttttta aagctgtcat acaatcatct gttttatact tttttacttt tgatttttcc
     2581 agaccatccg tcatatccac ccttaagtaa ataaatctct tgataacctg ctttacgtaa
     2641 tttatttgct gtacggatgc tcaaactttt cttttgatcg taaatataga caggctggtc
     2701 ttttcgaata gatcctaatg aatctttcaa aacagtgtaa gggatgtttc ttgcacctaa
     2761 aatatgccca gcatcaaaca catctttttc gcgaacatcg attacttgtg cttttctcat
     2821 tccttctcga aattcttctt ctgtcaaagt cgttgctgaa cgtttggcca ttactcgtag
     2881 atagacttcc caaaggatga ttgcaagcac aatcaatagc aaaatgatat taattaccca
     2941 taaaaccatg taatgcctcc gtttttttaa gcaaatttca aggctaatga ggcagcacca
     3001 atgacaccag cttcattacc aagctctgct aatttgattt ttgttgattc tcttacttgc
     3061 gggaaagtaa attctttaaa atatttctct acacggctac gtaaaaattc tcctgctgcc
     3121 gatactcctc cacctaacac gatacttgac ggatttaatg tattccccaa gtttccgcaa
     3181 gctaaaccta agtagaagca tacgcgatca acaaccatca atgcaaacgg atcgtcttct
     3241 tgagccattt caaagatatc tttacttgtt acttcttctc cgttgtctag cattgctttc
     3301 agttttgaat cccctgcgta ttcttctgcc aagaaacgac ctaggcgtac cacacctgta
     3361 gcgctagata cagtttctaa acatccgcgt ttaccgcaag tacattcgaa cccttcagga
     3421 tccacagtga tatgtccgat ttctcctcca gctccgctca cgccgtgaat caaatttccg
     3481 ccagcaacga tacctccgcc aacacctgtt cctagagtaa cgaatacaac gtctggatcg
     3541 ttttctccag cacctttcca tcgttcacct aaagcagcaa cattcgcgtc attatcgata
     3601 gcgaatttga ttcctgttcc tgcctcgatc agttttttca caaattgaac ttctgtccag
     3661 tttaagttat acgcaccaat aaccgttcca gcttcgctgt ccacgcttcc tggggtaccc
     3721 atgccgattc cgataaagtc ttcggctttc atattatata aattcaaacg atgattgatt
     3781 gattcaatga tttcaggaac gatatgctta ccatcatcca aaatattggt atcgatgctc
     3841 catttttgct gaatctctcc ttctggtgtt aaaattgcaa attttgctgt tgttccacct
     3901 aaatcaatcc caataatctt cttgtccatt acaaatttcc tttctttcgg ctttcttcaa
     3961 ttcggtgctc tctttttaat atgcgccacg ctgtcatgta tgtatcatga tcaatcaaac
     4021 ggctatcata cagattacgc aattcgatag tcattaattc aatgtcatag atgcgtgcac
     4081 ccacatagat aaagatgcca aattttttga acagctgctg tacatcatat aagctctcca
     4141 tctttaagca cttccttcat attatacaag taaaccatat ttttttaaac ccagtaaaat
     4201 acatattaca aaaagaaata caaaaactag tgtagaaaga atcctttggt gtatcgaata
     4261 gcttgtctgt tgtttcggta gacccaaaac attccctaac actaatccgc caattagccc
     4321 accgacatgt ccccaaacat ctacagaggt atccagaaga ccaaaaacaa atgtcaacac
     4381 aataaataaa agaaaatgtc tgactagctg ctggatcgct gggttatgtt taaaatgaaa
     4441 ccctaagata aacaacgcac cgaacaaacc gaatatcgaa gtacttgcac caccagacag
     4501 aacattcgct tcgttaaaag caaagcttgc tgtatttccc attatcccgc tgaacatata
     4561 aatcaagaaa aaacgccaat ggccatagat tgcctcgatt tgctgcccca taaaatacag
     4621 tatcagtgag ttcattacga aatgcatcag tccaaaatgg atgaagattg gtgtcactaa
     4681 acgccaccat tcattgaagt gaacaagata aggaccatac attccggcat aataagggat
     4741 ctcaagtcgc ggaaataatt ccatcatgag gaagaccgct gtctgtatcc ctaaaaaagc
     4801 ataggtccat aacggtcgtc ttaaccatgc acgcaatttt atttgctgtt gataattcat
     4861 gaaaacctcc catttcattt tagtcaataa acaattgttg gatcttctga tcaaagctct
     4921 ccggttgcca ttgttcattc aattgttcac gaaaaacaag gctacatgtt tttttatgaa
     4981 aattatttaa ataccggtcg taataaccac cgccaaaacc gattcgataa ccggctctat
     5041 tgtagataat acctggtaca atgaggagat cgatcatctc cggcaaatag agcttctctg
     5101 attgtggctc aagaacacca aaactcgaac gtacaagaac ggactgttca tccatttcat
     5161 aaaacgccat ctgacgtttc ggcaaagttt taggtacaac tattttcttt tgttcttttt
     5221 ctgctcgttg gataaccggc tgtgtatcca gttcaaattc attcgataaa gtcgtcccta
     5281 tcacttttgc tgacttccag tactcgctgg cgaatagctg tttatagatt cgctgttctt
     5341 tttccttttt cttgccactt ttggccagat ttttcagctt ttcgattcct ttccggcgta
     5401 atcttacttt caattggtcc aaaaactcac ccttccttct ctcataaaat ggtataggaa
     5461 aaaaagaaaa atagcaatcg aactttcaaa taaatgcata aatagaataa aaagaagacc
     5521 tagaaatgat tggccttcct tttttaatga atttcaactg ttcagcgcgt gtttttgtct
     5581 gataaaaagt gcatacagaa aatatggcgc accaaaaagg gcaatcaaaa ttcctgtagg
     5641 tacttcccca cttggtagaa tcactctagc aaccatatct gcactaagca gaaaaatgga
     5701 accgaacaaa ccgctcagaa caaaggtcca gttattttct ttacgtacga ctagcttcgc
     5761 agcatgagga gctagtagtc ctacaaaaga taaactacct gtcaccgcta cactaccgct
     5821 tgccaaagca acagctaaca acaaaaggat tttttgactt ttagccacgt taaggcctaa
     5881 tccttgcgcg cgttcttgac caaatgatag caatacaatc ctctgtgcat aaagtcctcc
     5941 tagcggaatt aagataaccg cccatggaag caatgcccaa acataaggcc agctcgcacc
     6001 ccagattgat cccgctaacc atgcattcac aaatccataa ttttccttag ataattggat
     6061 cgtgcatagc aatgttaatg cagaaaggcc ggcattaacg gcaataccgg acaataagat
     6121 tcgattcata cccaaaaagc catttcgatc ataggccgca agatagacac agcaagcagc
     6181 taaaacacca cctagaaagg ctacaactag caatagaaac ggcagcgaat agtgcgaaaa
     6241 gaaacctaaa taaagcatca ctaaaaatcc tgctccggca ttaatgccca atatactaga
     6301 atctgccaat tcatttcttg ttactccttg caagatataa ccagaaacgc caagacaagc
     6361 tcctgctaat aaactgatca gtaatcgagg aactcgaaat tctctaacaa tcagcgccaa
     6421 agaagatgat tctttcccta tgaacgtggc aaaaagctga tccaggtaaa taaaaggcgt
     6481 accaatcatt aaactaccta tagacaggat gacaagtaca aaaagcaaga aatagaacgt
     6541 ccaatatatt tttttcaccg ttgttccctc cttacttgta tcagtaaaaa cggaacgcca
     6601 atcaatgcaa tgactaatcc aaaaggtgtt tcaaaagggg gattgatcat cctcgctgta
     6661 aaatccgcca acataaccaa tccaccgcca actaaaaaag agacaggtag aacataacga
     6721 tagtccgttc caaccattcc gcgaacaact ggcggaacca acagcccaat aaatgaaaca
     6781 ggtccgacta acgaaacggc aatggctgct aacactgcaa ctagacagaa aacaatcaac
     6841 cgcgttcgtt taggatgact tccaagagct aatgcatgtg tttcactcaa atgaatcaaa
     6901 gatattgctt ttcctaatga aaaagaaacc aaaacagcaa gtatatataa aggaaacaat
     6961 actgctaatt gtttccatgt gatattggca gctccgccta caaaccaaaa agcaagatcc
     7021 tgctgcagat taaagaattg ggtaagtgct tggctgatag cagtaaaaaa agaactaata
     7081 gctacgccag ctaaaatcag ctgtaaagga ttcatgccca ttttcgaaaa ctgcaaaaaa
     7141 ccaaatacga gcaaagttac agcaaaagaa cctgccaatg acattagaaa cacagaaaaa
     7201 tcagaagctt taggccaaaa agcaaaacta agggccattg ccaacgaagc acccgcgttg
     7261 attcctagta agcctgaatc tgctaaagga tttcttgtca ccccttgcat caatgcaccg
     7321 ctcaatgcaa aacaactccc tacaagaaat gctcccgcca cacgaggcaa acgaatattt
     7381 cgtatgattt ggtgactttg ttttgatcta tcaaaatcaa acaacgcctg ccaaaaatct
     7441 gctttttcca cagtcgctgc gccatttgaa agagcaacaa aaaaaataat caaaagaaca
     7501 aaacagacca aaagcaggag tctatttttc ttcatagtcc tcataattga tcctcctgct
     7561 cttttggcaa caaatcatag gttagaaaaa ctggtttatt tgttcttgga agcgtgacaa
     7621 tcgttgcatc aatctcaaat atttttttca tacactcttt tgtgatcact tcttcaggtg
     7681 taccttctgt gatcaactta ccgtttttca ttgctaataa ataatctgaa aaacgtgaag
     7741 catgattaat gtcatgaatc gacatcaaga tcgttgtttg tttcatttga ttgatttcag
     7801 tcaataactg taggatctct aattggtgtg ccggatctaa aaatgttgtc ggttcatcca
     7861 agatcaagat atctgtttcc tgagcaagcg ccatagcgat ccagacacgc tgggtttgtc
     7921 caccagacag cgttgcgata tcccgcatac gaaatccagc tagacctgta gcatccaacg
     7981 cccactcaat tgcttcatga tcttgcgtgt ccatgccact gaacagtttt tgataagggt
     8041 atcgtccgta agatactact tcttctactg tcatacctgt agggtgttca ctagtttgtg
     8101 ctaataatgc aatttttttt gcaagttctt ttgaactgta tgtagagata tcagagttat
     8161 tgacaatgat ctttcctgat ttcacaggta gtatacgggt aaaacttttc aataaagtag
     8221 attttccact tccatttggc ccaatcaaac tagtgatttt cccttcagga atcgtaacag
     8281 acatgttttc aatgattttt ttgttttcgt agccggctgc aatgtcagct gctcttaatc
     8341 tcgtcatttc acccatcctt tcgctgaaaa tcattctcaa ttatgacgct gtttcaacat
     8401 ttcgtcaagc gctactttca tatctatagc caaatgaagc ggttgacagc gttcgaaata
     8461 tcaggaataa taaccaatga gaaagattct caattatgtt aggaggaaag aaatgaaatt
     8521 aaaaacaaaa gtattcacat tatctagcat attgctgctg gctggagtac tgagcgcttg
     8581 ctccccctct tcagataaag aaacagctac aactgccaat tcgacgactt catccaatag
     8641 tactcataca gtcacagata cacttggaca taaagtagag attccaaacg cgcccaaacg
     8701 gatcatcggc agttacttag aagactatct gattgcacta ggggaaaaac ctatcgccca
     8761 gtggactgtt ggcagtggtt ccattcaaga ttatctgcaa gatgatttaa aggatgtccc
     8821 aacgatttct tatgaccttc cttatgagaa agtgttaagc tttgagcctg atttgctgtt
     8881 gatttcttct tccgctactg tcgaaggcgg gaaatacgaa caatatagca aaatcgcgcc
     8941 aacttatgtt gtaaaaaacg gaacagatgt cacatgggaa gaacaactaa aagatgtcgg
     9001 caaagctttg gcaaaagaaa aagaggcaga aaaaatcatt tccgattatc aaaaatccat
     9061 caagaaaact cgagaagaac tagcagataa aatcgagaac aagacagctg cagtgttgtg
     9121 ggtgaccaac aactcagcct ttatggtcag caaaaatcgg tcaagtggaa gaatcgtata
     9181 cgacgatctg gaatttggtg ttcctgatct agtggaagag gtctccaaaa aagccacttc
     9241 tgactggtct gctgtttcct ctgaaaagct agctgaatta gatgctgact acatcatcct
     9301 tgtgaacagt gacaaagatg ctgccatgtt caatgaacca agctggaaga atctaaaagc
     9361 agtgacagac aatcatgtac tgcaattcgg acctgaatct agttggctat ataacggacc
     9421 tattgccagt caggaaatgg tcaatgatat caaagaaaaa ctaaattgat tttaactaaa
     9481 aaaagcatct tcacgattag tcgtgagatg ctttttttct tattaacaaa ctagacgcat
     9541 agattttatt cttcttttga agcaacccta ttttttactc tttcccattt ttgaaaacga
     9601 tgggcatata gattttcttg atccgttatc ccctcatact cctctataag ggcgaagtct
     9661 gaaaaatcaa tattcccaat atacgtatcg ccttcaaacg tgctatcgat caatgttcgc
     9721 cagatccgac cagtgtaagg aagaagttct tgaaaaatcc gactaccacc cgaaacatag
     9781 atatcttctg agatcgtttg actatacgac aaaagttcat cgatcgaatg cattacttcc
     9841 gcattttctt tcgtgcgaaa atttggttgc gttgtcaaca cgatgatatg gcgataagga
     9901 agtgaaagat cccccattcc ttcataggtc tttcttccca tcactaaaat tcgatccatt
     9961 gtatgttcgc gaaagaaacg catatcattt ggcaatcgcc atggtaacaa accatttttg
    10021 ccgatcagac catttttatc ttgtgcccac atggaaataa acatattatc cgctccttat
    10081 gattttagat gttttgagat aaaaaaagtc tcaattcttt gaccagcaag gaattgagaa
    10141 ctttaaaaac attattttgt ttcacggtgc aaagtaactt tgcgttcgcg tgggcaatat
    10201 ttttgttttt ccaaacgatc aggattgtta cgtttgtttt tgcttgttag atagttacgt
    10261 tctttacaag aagtacactc taaagtgatg tttacgcgca tgtttttccc tcccattttt
    10321 acaagaatcc tctaagatga ctctcagact tgattatctt accatgattt tttctagatt
    10381 gctagtgttt ttcacgattt tgttaagtaa aactacttga cgataaatac aacggtttta
    10441 cttctcaaac aaaatctttt tctttttgta taaataccct tacacttgtt tatatagatt
    10501 atgtttttta ataaaggggg atactaatat gccatggaac atgaaggact ttcctgcatc
    10561 aatgaaaaac ttagataaat tgacaagaaa aaaagcaatc gacatcgcaa atgcattatt
    10621 agatgaaggg taccctgata accgtgcgat cccaattgcc attgatcaag caaaaaaatg
    10681 gtatgaagat gctagtgaat cagaaaaacg aactttcgaa aaagaaaaaa atccaagcaa
    10741 gacggatgaa cacgatacga atcctcgtgc tggaaaactg cttgattcag atgtgatcgt
    10801 agaatatgag gaagaacaat ggatcgtgaa atctaaaggg gcaaaaaagg caagcaacca
    10861 ttttgataca aaaaaagaag caatcgaaaa aggaaagcaa gtagcacaaa acaaagaatc
    10921 aactttgttc atttataaaa aagatgggac aaaagaaaaa gaaatcgatt attgaacata
    10981 tgtaaagggg ttgtgacgaa aatcgtgtca caaccccctt atgttcgaaa caatggtgtt
    11041 cctctgccaa ctccgaacca taagtcataa tatctataaa acgggaagag ctgttttttg
    11101 aaattgtttc ttatcactca gtgttaaacg gtaggagaaa acctcttttt tattgatatt
    11161 tgattttcat caaacagtca catagtcgat tgcttctgct tgcggaatcg tttcatctac
    11221 aaagtagact ttataccatg tcaagaaact gaagattgtc cagatttttc tttgctcttc
    11281 tttacgtcct tcgtgatggt catctagcaa cttcatgatt ttttcttgat caaaaaattc
    11341 ttttgcaaaa tctgcttcga ataatgcttt gacttgttcg tagccgtgtt cttctttcaa
    11401 ccaagctttg attggcacag ggaatcctaa tttcacacga ttcgaccatt cttccggaag
    11461 atgtttgttc gcagcttgtc tgaaaatatc ttttgtgttg ttttgattca ataaaaactt
    11521 acttggtatt tgttgagcca atttcatgac ttcgatatcc aataaaggta ctcggacttc
    11581 caaagaactt gccatagaaa gtttatctgc ctttaacaag atatcttttg gcatccattg
    11641 atgaagatcg acatattgca tcttattgac ttcatctttg attccttctg ttttctcgta
    11701 atgaacagtc atgatttcat ccacagatgg tgctgtttga tattttggtg ttaacacttc
    11761 tgatgcttct gattcttcga acactcgagc atgaccgata aaatattctt ttgcaggagc
    11821 aagtgattca tataaatgga tccgtccatg gaagtttttc atttttccga ttttacgtcc
    11881 taaattatag cgtgttcctt ttggtaattt tttcaatcct tgagcaatca cacggatgaa
    11941 ttttgaattc gtatggaatc catacgtttg gtatcccgca aacagttcat ccgccccttc
    12001 tccagattgg actacccgca cactttgtga agcaagattt gctaaaaagt acaaaggtac
    12061 gcaagaagga tttgaatcag gttcatctaa gtgatactgg atcaaaggga atgctttgaa
    12121 cgacatgtct ccgtcgatca ctgctgctgt attatctaag tccaatagtt cggttagttt
    12181 tctggcttca atcgcttcat tataggtttt atcatcaaac ccgattgaga aagaatgatc
    12241 tggtttcaat acagaggtta cataactgga atcaaccccg ctggataaga aagaaccaac
    12301 ttctacatca ctgaccgtat gtgcttcaat agaagcttga acggtttcgt cgattttttc
    12361 gatccattct tgtcgggaat gcgtttcttt tgtttcaaaa tctgcatccc aatattgctg
    12421 aatgtctatt tttccatctt tatacgtata ataatggcct tcaggcaaac gatagacatc
    12481 tttgaagaat gtttcgccgt ttaatggaga atattggaaa gtcatataag gtttcaacgc
    12541 ttctttgttc aattctttgt taaaatcagg atgaggcaga aaacttttga tttctgaacc
    12601 aaacataaat gttccgttca tttctgcata ataatatggt ttgatcccaa agtggtctct
    12661 tgcaccgaat aactcattct tttcattatc ccaaatagca aaagcaaaca ttccgcggat
    12721 cttttgcaat aattctgttc cccattcttc gtaaccatgc aaaaggactt ctgtatctgc
    12781 atgtgtttga aaaacgtggc cagctgcaat caattcttct ctcaaaggct ggtaattata
    12841 gatttcgcca ttaaaaataa tgatctttgt ccgatcttca ttgtaaatag gctgagtacc
    12901 gccctctaag tcaataatac ttaatcttct gaatcctaaa gcaacatgtt tatcgatata
    12961 ttctcctgaa ctattaggtc cacggtgaac gattcgatcc atcattgtat taataattgt
    13021 ttttttgttg tccttgtcgt taacaaaacc tacaataccg cacatttaac tttcacatcc
    13081 taatataaaa ttactgttta tttcattcat atgcatctta tcatagaata aatagcggac
    13141 gaatattttc tctcttacaa tttcgtcacg tgaaaacaaa agatgaaagt ttctgttttt
    13201 acagaaaata aagtcccaat atctaattta ccatgttttc ttttttgagg ctaccttatt
    13261 ctcttcgtgt taaaaaaata ttagtggatg ctatttccaa aaaaaccagc aaaaaaagac
    13321 acgacctaaa gatcgtgtc
//
LOCUS       DACDMM010000070        13320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDMM010000070 DACDMM010000000
VERSION     DACDMM010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.070"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..225
                     /locus_tag="IWD16_001575"
     CDS             <1..225
                     /locus_tag="IWD16_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088638.1"
                     /translation="VFLAQEQKLVPVTMVQINKANQFIFLFEHGKAPLAMKDIFLFLM
                     KNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            641..2179
                     /locus_tag="IWD16_001576"
     CDS             641..2179
                     /locus_tag="IWD16_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP9088639.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            2176..3141
                     /locus_tag="IWD16_001577"
     CDS             2176..3141
                     /locus_tag="IWD16_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9088640.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            3128..4450
                     /locus_tag="IWD16_001578"
     CDS             3128..4450
                     /locus_tag="IWD16_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9088641.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4513..4617
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4691..5434
                     /locus_tag="IWD16_001579"
     CDS             4691..5434
                     /locus_tag="IWD16_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9088642.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5449..6441
                     /locus_tag="IWD16_001580"
     CDS             5449..6441
                     /locus_tag="IWD16_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9088643.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6453..7190
                     /locus_tag="IWD16_001581"
     CDS             6453..7190
                     /locus_tag="IWD16_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9088644.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            7180..7902
                     /locus_tag="IWD16_001582"
     CDS             7180..7902
                     /locus_tag="IWD16_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9088645.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7986..8609)
                     /locus_tag="IWD16_001583"
     CDS             complement(7986..8609)
                     /locus_tag="IWD16_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9088646.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            8944..9378
                     /locus_tag="IWD16_001584"
     CDS             8944..9378
                     /locus_tag="IWD16_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9088647.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            9401..9721
                     /locus_tag="IWD16_001585"
     CDS             9401..9721
                     /locus_tag="IWD16_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088648.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            9862..10053
                     /locus_tag="IWD16_001586"
     CDS             9862..10053
                     /locus_tag="IWD16_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088649.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(10124..10381)
                     /locus_tag="IWD16_001587"
     CDS             complement(10124..10381)
                     /locus_tag="IWD16_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9088650.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            10539..10802
                     /locus_tag="IWD16_001588"
     CDS             10539..10802
                     /locus_tag="IWD16_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088651.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            10826..11014
                     /locus_tag="IWD16_001589"
     CDS             10826..11014
                     /locus_tag="IWD16_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088652.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(11097..11282)
                     /locus_tag="IWD16_001590"
     CDS             complement(11097..11282)
                     /locus_tag="IWD16_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088653.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            11497..11868
                     /locus_tag="IWD16_001591"
     CDS             11497..11868
                     /locus_tag="IWD16_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9088654.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(12018..12090)
                     /locus_tag="IWD16_001592"
     tRNA            complement(12018..12090)
                     /locus_tag="IWD16_001592"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12055..12057),aa:Gln,seq:ctg)
     gene            complement(12136..12564)
                     /locus_tag="IWD16_001593"
     CDS             complement(12136..12564)
                     /locus_tag="IWD16_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088655.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(12624..>13320)
                     /locus_tag="IWD16_001594"
     CDS             complement(12624..>13320)
                     /locus_tag="IWD16_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP9088656.1"
                     /translation="GTSYYLHIHSPEHTMNTAQKSSTQKTKKTKEKAEVLLDVPLESQ
                     FDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDYVPVFNADGTHGDPNEGFVGDI
                     SGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLDDIKKALSKGNPVWTSVTINFE
                     VPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIYVNDPYGVKNRAVPINDFSAIF
                     TAMGGQMLTLEKS"
BASE COUNT         4427 a         2212 c         2786 g         3895 t
ORIGIN      
        1 gtttttttgg ctcaagaaca aaaactggtg cctgtgacaa tggtccagat aaataaagca
       61 aatcaattta tctttttatt tgaacacggc aaagcaccac ttgctatgaa agacatcttt
      121 ctttttttga tgaagaaccg tgagaaagct gtttattatc agtcaaaaga agaactcatt
      181 cctctttatg gagtgaaaga gatagaaaat aaagtggtga tataagtaaa aataagaaaa
      241 caagacgttt atgatgtcaa aacaaccatt ttttacattt ttggcaaaaa agtgtcatga
      301 tacagtataa tattcgttga acaggccaat aaaggtgttc tcccccacga gatttctact
      361 ttattggctg tttcttttta ttaaaataaa agttgtgtgt gtaaaaaaga tatagctgat
      421 tccaagtgac aatttcgtat agatttttat ggtattcgta gtgaaaaaac gagaaccaaa
      481 aagttgatcc gactttgttg cttggattct ttttttgtca gcatatttag aggtattttt
      541 ttcttttttt tgttcgtgct gtaaggttaa ggcaagacaa gttagttggg aaaaaggaaa
      601 gaaatgttta tacttaaaga ataacagaaa ggaaggtcgc atgaataatt ttatacaaac
      661 attaagtgaa agaaaaggcg agttgctgga agctgcattt caacatctgt ccatttctct
      721 catttctttg ctgattgcag cactgattgc tattccttta gcgatttggg cagccaatca
      781 taaaaaaatg gcagaagtct tattgcagat cacgggtgtg ctccaaacga ttccttcact
      841 tgccttatta ggtttactta tcccatttgt agggatcgga actgttccag cattgattgc
      901 acttgttatt tacgctttgc ttccgatttt ccaaaacacg tatattggtc tagctgaaat
      961 tgacccgtct attgaagaag cagcggtagc atttggtatg tccagaatga gacgcttgct
     1021 gaaagtagag cttcccatcg cattacctgt cattatctca ggtatccgaa ctgccttagt
     1081 tttaatcatc ggtacggcta ctttggcagc attgatcgga gcaggtggat tagggacatt
     1141 tattttgtta ggtattgacc gaaatacacc tgttttgaca ttgattggtg cgatcagttc
     1201 tgccttgctt gcaattatat tcagcggact gattcgctgg ttgcagcata agaaacctcg
     1261 atattccatc attactttag cagcagtggt gtgtttattt ggcggcgttt ctttatatca
     1321 gtctgccttg tttaaaaatg aaaccatcac gattgctgga aaattaggag cggaacctga
     1381 aatcctgatc caaatgtata aagaattgat tgaagacaac acaaaaacag aagttgaatt
     1441 aaaaccgaat tttggcaaga cgagtttttt gttcaatgca ctggatcaag gtcaaatcga
     1501 tatctatcct gagttcacag gtactgtcct agaaagcttg gtgaaggtac ctgaagcaat
     1561 gaaaagtgct gatttgacaa aagaaaaaac atatgacgaa gcagaagaat tattgaataa
     1621 gcaattcgat atgacattat taaaaccaat ggcttaccaa aatacgtatg ccttggcagt
     1681 caagagatct tttgcgaacg aacatcaact taaaaagatt tccgatttgg cagctatctc
     1741 tgatcagatc aaagcgggat tcacactgga atttattgat cgttcagatg gatataaagg
     1801 aatccaagaa gcatatggct tgaatttttc aagtgtacaa agcatggagc caagtttgcg
     1861 ttaccaagca atcaataatg gagatgtcaa tattgtggat gcctattcca cagatagcca
     1921 gctcaaggag tacgatctag tgactctaga agatgatcga gggttattcc cagcttatca
     1981 aggcgcaccg ctgatgaaaa aagattttgc taaagaacat cctgaaatcg ttaaagcact
     2041 gaataaatta agcggaaaaa taacggagga tcaaatgatt gatatgaatt accaagtcaa
     2101 tgtcgaaaag aaaaagccga cagaagtagc acgaatcttc ctgaaaaatg aaggattagt
     2161 aaaggaggaa gatcagtgac taaattaatc gagttcaaac atgtgcaaaa aaagtatgat
     2221 ggaaagtatg tcatcgatga cctaaatctg gcaatcaaaa aaggagaaat ctttgttttg
     2281 gttggtcctt ccggtagtgg aaaaacaact actttgaaaa tgatcaatgg attatctaaa
     2341 ccatcagcag gagacatcta cttcaaagga aaatcgttaa atgaatataa tctgcagaaa
     2401 atgcggtgga atatgggata tgtgctgcag caaatcgctt tgttcccgac catgacagtc
     2461 aagcaaaata ttgaagtcat ccctgaaatg ttaggttggg aaaagcagaa aagagcagac
     2521 cgtgtagatg aacttcttca aaaagttggc ttatctcctg atatttatcg agatcgtatg
     2581 cctagagagt tgtctggagg agaacagcaa cggatcggga tcatccgagc tattgctgca
     2641 agcccagatg tgattttgat ggacgagcca tttagcgcat tagatccgat ttcaagaaac
     2701 tcattacaag aattggtttt gtccttacac gaagaattgg gcacgaccat cgttttcgtt
     2761 acgcacaata tggaagaagc aatcaagcta ggtgatcgta ttgcatttat gaaggatgga
     2821 gaaattatcc aatgtgacac accagagcag ttattgatga atcctaaaaa cgactacgtt
     2881 cggcatttct ttgatgaacc gaaacagacg aaagagtggc gagtagaaga tttagtcgtc
     2941 aatggatatt tcttaaatga aataccagaa aatgctcgtc aacaagtttg ctttgatacg
     3001 cctatgaaag aagtatattc gcttctttcg gtgtatccaa gcattgtgat agtagaaaag
     3061 caaagaagta ttgggtcgct gacaagtaaa gaaatctttg cttttttaag tcgagaggag
     3121 gaaggaaatg aaaacatatg atgcaatcat catcggttca ggtgtgagcg gcttaagtgc
     3181 tgcttatgga ttgaaagaag caggtaaaac agtactagtg gtcgaagaag atctatgggg
     3241 cgggacttgt cctaaccgtg gatgtgatcc taaaaaagtc ttgttaagtg cagtagaggc
     3301 acgtaatcgg gtaaaacaat tgagtggaaa aggttttaac gagatcccaa ctgccaactg
     3361 ggaagaactt caaaaattca aacgaacatt tacggatcca gtcccagaaa gccgaaaaaa
     3421 acaattagca gaagctgaaa tcgatcatct ttcaggaacc gctcgttttt tggatgattc
     3481 atctattgaa gtcaacgaag aagtcttcca tgcggattac ttagttttgg caaccgggca
     3541 aagacctacg atcttaccag tggagggaaa ggaatacttg aaaactagtg cagacttttt
     3601 atcattgcct gtactgccta aagaaatcat ttttatcggt ggtggttata ttgcttttga
     3661 gctagctacg attgccaatg ctgcagggag caaagtaacc attgtccatc ataatcaacg
     3721 gccattaaaa gaatttgagg cgtctttggt agaggaagcc gtccatcaaa tggaagcatc
     3781 tggcattcag tttgcttttg gtgttgaaac ccaaaagatc atttcagaag gtactcgcta
     3841 tcgtttagtc ggaaaagaga cggaattagt agcagatatg attttttgtg cgactgggag
     3901 acagccgaat acagaatctt tagcacttga gcaagcgaat atcgtctttg ataaacacgg
     3961 aattgcagtc aatgattatc tgcaaacgag caatccaaaa atttttgcgt gtggagatat
     4021 tgtttcaagg aaaaccccaa aattgacacc agtcgccact tttgaaggaa attatgtagc
     4081 taagcggata acggatgcaa caagcgaacc tatcaaatat ccgattatac ctacgatcgt
     4141 ctacgccagt cctaaacttg ctgaagtagg agttacaaaa agtcacgcct cttcgtcaga
     4201 tcaagtggta gagatggatc ttaccagttg gttcacctat catcgtgtca atgaaccagt
     4261 tgctaaggct gaattgacct ttgaccagca aaattatttg attggtgctg cagtgattag
     4321 cgagcaagca gacgaattga tcgatgattt gacactcgtc atcaatcaaa aattgactaa
     4381 aaaagagttg gatagctata tcatggggta tccgacattg gcaagtgatc tctcctattt
     4441 attgaaataa tcagcatgat aactaaagcg cttgttgctt tttccttctt gtgctacact
     4501 ttaaaaaatg aatacctgtt tctggggatg tcgtagacga catgagagta agttgtgaac
     4561 ttagacccat tgaacctgtc agttaatact ggtgaaggga gaaacatagc agacgcttga
     4621 agcgggttac tatgtcgtgc ccatttcaag cgttttttgt tattcatatt agaagaaagg
     4681 aggtaacaaa atgaagaaaa atctttatcc agcagtcagt atggcattca ttttaattat
     4741 ttggcagatt gttgtcatag tttggaaaat acctgccttt attttacccg gacctgctgc
     4801 tgtattttct gcattagcgg cagatgcaca agtattactt tcccattctg tcacgacatt
     4861 aaatgaagca gtgcttggtt tggtaatagc tagcgttctt tcttttatta ctgccttggc
     4921 aatggattat tggaagtttt tagaaatgtc tttttatccg ttactagtga tttcacaaat
     4981 gttgccaatc atggtgttag ggccattact taccctatgg tttggtttcg gtatgcttcc
     5041 aaaagtgatc cttgttgtct tgataagcta ttttccgatc gtagtttctt tttcggatac
     5101 attgaggaaa gtatcaaaag agcaaatcat ctttttgaaa acgatggggg cagatacagg
     5161 gaagatttat cgaatatata aaataccagc gggtttatcc ggcttctttt ccggtttaaa
     5221 agtggctgct acttattgtg tcagcggagc agtcgttgga gaatggctaa gcgctcaagc
     5281 aggcttaggg tattacatga tacgcgtaaa aaacagctac cagatcgata aagtttttgc
     5341 tgcgatcatt tgtgtcattc tattgagttt attgctgaat ggcgcttgtt cattattgaa
     5401 aaaagggtat tatcgtatgc tttatatttt ttaagagaag gggatcaaat gaaaaaatta
     5461 tggcttttat taccgctact gttgctttta tccgcttgtg gcactgcaaa agaaacaagt
     5521 agcaaacaag aaataaagga tttgaaaaaa gtgacacttg ttttggatta tgtaccgaat
     5581 actaatcata ctggcatcta cctagcaaaa gaaaaaggct attataaaga ggctggttta
     5641 aatgtccaaa tcatcgaacc aggagataat tcgacaagta ttggtctagt aggtgcggac
     5701 aaagcacaat ttggtgtaag ttatcaagaa gatgtcactt atgctcacgc agatggacag
     5761 aatataccag tcaaagcaat tgctacagtg atcaaacata atacttctgg attcgccacc
     5821 ctatcagaca gtaacatcca ttcaccaaaa gattttgaag gaaaaacata tgcaggttgg
     5881 caaagtccaa gtgaagaagc tgtcttaaaa gctgtgatgg aaaaagacgg cggggacttt
     5941 tcaaaactga caatggtagg aagcaatgga gaagggccag aaagcttagg aaaatcctca
     6001 gatatccaat ggtactttga aggttgggat atgatcaaag caaaagaagc gggtattgaa
     6061 ctaaactata tcccattgaa agaattagat gaacggttag attactatac tccggtaatt
     6121 attacgaatg atcaactgat caaaagtgat ccagaattgg tacagtcttt tatggatgcc
     6181 acgaaaaaag gctatcagga agctattaag gatccaaatg acagtgctaa actgttgcaa
     6241 aaatatgcaa aagaaaatga tcggacgttt ttagaagaat cacaagcatt cttgtcaaaa
     6301 aattatacgg atgatcctga gaattgggga ttgatggaag aaaaagtttg gcgcaattat
     6361 acagctttta tgcaggaaaa cggattgatc aagcaagatg taccttcgca agaactattc
     6421 actaatcagt ttataaaata ggagtgatag atatgtcaat ccaaatcagc cacttgaatg
     6481 tagtcattga aaacctgaaa atattagaag acatccattt cactatccct aaagatacct
     6541 ttacatcgat tgtcgcgcca agcggagccg gtaagtcaac attgctgaaa actttaacag
     6601 gagtccagcc tatgacttct ggaacgatca aagtagacga tcaaaaagta actggtctcc
     6661 atacagcatt tagttatatg ccgcaagaag atatgttact tccttggctg agcgtctatc
     6721 aaaacgttac gttatatcaa aaaatcaatc atctaacaat caatgaagaa caagtaaggg
     6781 attatctgga tatctttggg ttaaacggct atgaacattt tttgccggaa caattatctg
     6841 gcgggatgaa gcagcgaacg gccttgttac gaacaatcat gaatccttct tcttacttgc
     6901 ttcttgatga accatttgga gcgttagatg ctatgaccag agggcagatg caagactgga
     6961 tgttgaagct gcctaaaaaa gcaaagcgga ctactttact tgtgacgcat gatatcgaag
     7021 aagcaattta tttatcagac aggattcttg tgctttccgc ccgtccggca catgtgattg
     7081 ctgagattca agtgcctgaa aaacgacggt cacgtgaatg gctactgcag caatcagagt
     7141 tgaagcaaac gatttatcag ttattggcag gtgaattaga tgttaagtga tgctcattgt
     7201 catttagatg ggagtcagag tttagcactc cttcagcagg aacacacgat tttgacgatc
     7261 atcaattgtg attcgccaga agaatggaag gaaaacagac agcttgcagc aagtaagact
     7321 caagccttaa gttacggtat ccatccttgg aaagcagata gctatacgtt tgaacaagtt
     7381 gagccttttt tgaaaaaagc aaggatcatc ggtgaaatag ggttagataa tatttggacg
     7441 aatgtaccga tgactacaca aaaaaaagta tttgagcgac agcttgcttt tgcagccata
     7501 aatgagaaac ctgttgtact gcacacaaaa ggatgcgaaa aagagatctt aaattatatc
     7561 caagcttacc ctaatcacta tttgatccat tggtattctt ctttggacta tcaacaagac
     7621 tatattgatt taggctgtta tttcagcatt ggtcttgatt tgaaaaagaa tccagcagtc
     7681 tggcaactag ctaaagcagt cccaatcgac cacttactta ttgaaacgga tggactacat
     7741 gcagcagagt gggctttagg ggggattttt tcagaaaaag attatgtctc actgcttgaa
     7801 gaacagttaa agacaatcgc tgagatcaaa gagctatcag ttgaagaagt taaaaaaaat
     7861 acttatcaaa acttggaacg tttcattaga ttaggtaaat aaaaaagaga tgtgacacaa
     7921 ctagtgtcgc acctcttttt ggctttcttt gtataaaagt aaactgtttg attaataaat
     7981 gaatcttagt accaaccgtt tgcttcccag aatgctttcg ccgcttccca agaaccataa
     8041 cgtgaagtaa catattgttc tgctactctt tcttggtttg cagcagagta gtcaccattt
     8101 aagtaagaag aatctaattg gtaacgtccg atgtaacgtc cgttcgttgc tgtgtaagaa
     8161 ccgcttgatt ctttttgtgc gatccattct tttgcagagc ttgtgcttgc tggagtagca
     8221 gcagtttctg ttacagcagg agcaggttgt gtttcagcaa caggcgctgc ttgtttttcc
     8281 gtttgaaccg tttcttgtac aacaggttgt tcagcttgga ctggtgcttc ttgaacggct
     8341 gcttgaggtt cagcagctgc ttgtgcagag ccttctgttg ggattgttaa ttgttgtcct
     8401 gagtaaatca ggttaatatc agaaattgaa tttgattctg caatggcatt gatcaaggag
     8461 ttatcaccaa cgtatttttg agaaatcgtt gatagagtat cacctgattg aacggtataa
     8521 gcttcgtccg catgagcagt cgttcccatg aagaatgcag cgccggcagc caaagttgtt
     8581 ccaaaaagta aagttttaag tgatgtcatg aaaaatctcc tttattcgtt aaaaagtttt
     8641 atttgttgtg ttcaacgagg aatactttaa cacgtgaaaa tatttcatag gtaaaagaag
     8701 catgacaatt tatgacaaat ccacgttttt tgtaacagaa tgatgttcgc tcgatgtgaa
     8761 aaatgatgag gaatttcaca taatgcagaa ataatgggat tagaacaggt gttctgatga
     8821 gaaaagaaat gtataaataa cttgaaagaa attgcttttg ttcatcattt tatttacata
     8881 ttgcgtatat tttttgtatt tttgcttgag atgttacaat aaaagaaaaa ggaggcggtt
     8941 ccaatgagta aaagatacca gaacatcatg gtagcagttg acggatcaag acaatcgatc
     9001 aaagcgttcg ctgaagccct tgacttagca aaggacaatg aagcccactt atttatcgtg
     9061 tcaatcatca acaaagttga gctgacccat agtgcctatg ccttttcaaa aatctatgca
     9121 gatgaaaaac aaagaacaga agtagccatg ctgaaaaaaa tcaatgatgc caaagagtac
     9181 ggcattgctg agatccacgc aatagttgaa actggagatc caagaaattt gattgccaat
     9241 gtcatccctg gacaagaaaa tattgatttg atcgttatgg gagcgacagg aaaaggtgct
     9301 atacaacaag cattagttgg atcgaccgct tcttatgtcg tatcacatgc cccttgcagt
     9361 gtgttggtcg tgaagtaatg agataaggga ggaatagaaa atggttcgtc ctcaagaagt
     9421 ccgtgcgcca aaagaaaaaa tagaaatttt agcaattatt gaagacggca cacaaacaaa
     9481 gaagggatac tcgatcgctt tagtaaagtg gaaaggaaaa aaaggtgttg ctctgagatg
     9541 ggatggagat aaccaacaag ataaaggatt ccctgtcaca gctaacggtt atcatccagc
     9601 gtggtttgtt ctgcctgata aatttatgga attatacagc tatgattatg caagagccgc
     9661 agcatcaatc aaagcattag ataaattaac agaagagcag aatgaaatgg aggaaaaata
     9721 aaaaagacga accaatcagc ctttagaaaa ctgattagtt cgaggagtaa aaatgaaaaa
     9781 aggttatttg ggatgaaact agaataatgc gtataactta aataaaactt aaagcaataa
     9841 aatggcaaga gggtgaggca aatggaagca ggaaaagtat tacaggaagt aaaagcacta
     9901 tgtgcagata aaaaatatga agaagcgaaa agcctgatcg aaaagaacaa agaaaatttg
     9961 gaagaaaagt acccgatcgc acaagaattt atagaattaa aacaaggaac gatccttcac
    10021 cgattcaaac atttttttgg tataacagaa tagaaaaaac gattaaagat cggcagaaat
    10081 ttgctgcatc tttaatcgtt ttctttttaa acatgcgagg gattcaattc tgattcttat
    10141 ataaaaaata gatccaaata ataagaaaaa ttactgaaag cgtcaattcg atgatgtgtg
    10201 ttattaatac agacgttgtt tcccaatgcc taaaagaaaa aatttgataa gtaaaatata
    10261 cagcaggtaa agaggccagt aaaactgaaa acttcttttt tgtccatttt atgtaataga
    10321 aaagaatgct tatcataata aaaaaaggaa tataaaaaac aaattgaccg atcgcgatca
    10381 tactattgcc tccgataaat aaagtccatg atacaaaata acactttgtg cgaaatggcg
    10441 caagcagttt tgtggattta tccggtaaaa tctgtatcga ttttgaaatt agaggagaaa
    10501 cttgctataa tcaaagtgaa agaaggagtg ggattttcat ggatcatgta gtgttagaaa
    10561 tcgatttgca tttgatcaat aatattcggg tgatttacta tgttgtaagt aacagtgtcg
    10621 aacaacgtgt actgacgaat aaaataaatg gtattttagc aaagaaagac gttcaccgat
    10681 tcaataatgg tgaaggaagt tattatagta tcccagtaga aaaaattatt tatacgacag
    10741 tcaaagtcag agaagatcta gataccaaaa aagcatatga acctattttt acaacttatt
    10801 gattgacagc agatggagga aaaaaatgaa tccatatgaa caacttattc aagatttgat
    10861 cgatggaaaa atcgaaaaga ttgatgtaaa aagagaagaa ttaatggctt ttcgagaagc
    10921 gtggctgaag ttggagaatc gtaaatatgt ggtgggaaaa gcctcactga acggcaatgt
    10981 gacttatcat tacgatccga cacgtttatt ttagtatgaa gtattcagaa aagaaccaaa
    11041 ggaggacgat gcagcagcat tgtcctcctt tgattttaca aaagtgaaag ttttacttat
    11101 ttgaatagat ttttaaattt gtttttgaca ctgtcgatat ttacatcttt taaaagattt
    11161 tgggctttct ctttataagg tcctaattgt tctttatgtt cttcgataaa tcgttttgcg
    11221 ccatcgaaat tgccgttctt aatcatttcc ttgacttttt gaaataattc ttcttgattc
    11281 attttgaaca catcctttgc tatcaacatg attttttaca tggacaaaac gagaaaaact
    11341 gatttatcca cacgtcaata atatcaaata acgatcattt ttactaaaaa aacactcgat
    11401 tctcgcttct aaagtaggag aaaaatctcc aacttgggac ggatggaatt tctttaactg
    11461 acccatatac tgaatatata acaaggaggt agaaagatga aagtgattaa atatttgatt
    11521 ggactggcgg cgattagtgg tattttgcta tttggactga aatattatac tgaaggaagt
    11581 tatggagaaa tccctggtat acttgatcag ctaaatccat tagttgaaaa aggagaggtc
    11641 tatgtaaaga cacaaaaacc agaggaagta aatgaatatg gtacagcaag gtatgtgcag
    11701 aaagctgctg atgccaatgg gaaagaacga acggttgaat ttaatggtct ttctgtgtta
    11761 aaagaaaatc attatttgaa aattagcaat aaaggagcac acatagaaac gtatgaggaa
    11821 gttcctagag aaaaagttcc ggagaaagca ttagcaatta ttgggtaaat cagtttttgt
    11881 attaagaata cgcccgattt tattattcat aatttaatta tgtaaactaa atacaagtag
    11941 taaaaataat acaataatcc ccctgcaaaa agattgttct tttacagggg gttcattttt
    12001 tcagcaagca catcaaattg ggatggcagg gttcgaacct acaattgctt gattcagagt
    12061 caagtgcctt accagtttgg ctacatccca tttattcaac aaaataatca tagcactttc
    12121 ctaaaaaaac gcaagttatt tttcttgatc ggatagttgt tgctttactc gtaaaaggcg
    12181 gcgtactagt ttcttttcta atccagcagg aagggcaatc agttctgcta ctgacaagac
    12241 ggtgtccaat aactgatatt ctttgacagg ttcattcata atatgataga tgtttgctaa
    12301 acgttcaata tattcaaagc gagtatactc tgttcgagca attagaaatt tcaataaatc
    12361 ggtggctttc tcgtactctt gatctgcgta ataaacaaga gattgacgat acaatttgat
    12421 ttccagttca ttgagggtag caggtctgcg acgctcgttc cactcattaa tagagacttt
    12481 accttgaaca tttcccttgc agtcacggac aaaaataatc ggaatttcat ttggatccat
    12541 ttttttatgt gtgaattttt ccattatcta cgctcccttc aaaggtttgg tatcttgagt
    12601 ataagggatt tttgtttgaa ttactagctt ttctccaaag ttagcatctg tccacccatt
    12661 gcagtgaaaa tcgccgaaaa atcgttgatc ggtactgctc gattttttac accataggga
    12721 tcgttgacat aaatattttc tttgtcatat ccagtgacta cacaagcatg aacaagagga
    12781 gtgactctta cttctccatt atttgtggtc caagtcataa aatcagtttc atctgggact
    12841 tcaaaattga tagtcacaga agtccagaca ggatttcctt tagatagtgc ttttttgata
    12901 tcatcgagtt gagcatcggt ttttggaaaa gtgtgataat ccgtttggac gatttgctga
    12961 gcaagggagg caactggggg aacatagacg accatagccc agtctccgcc actgatatca
    13021 ccaacaaatc cttcattagg atcgccatga gtcccatctg cattgaaaac agggacgtaa
    13081 tccaattgct cagctaactg gtttttagtt gtttcataac cataaaaaga taaaagcata
    13141 gaaagcgaag tgacttcaca gccattttct aaagaaggag aatcaaactg gctctctaat
    13201 ggaacatcta gtaaaacttc tgctttctct tttgtctttt tcgttttttg tgttgaactt
    13261 ttttgtgcag tgttcattgt atgttctgga gaatggatat gtaggtaata acttgtaccg
//
LOCUS       DACDMM010000071        13279 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDMM010000071 DACDMM010000000
VERSION     DACDMM010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.071"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(12..1535)
                     /locus_tag="IWD16_001595"
     CDS             complement(12..1535)
                     /locus_tag="IWD16_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9088657.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="IWD16_001596"
     CDS             1787..2968
                     /locus_tag="IWD16_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9088658.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="IWD16_001597"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="IWD16_001597"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP9088659.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="IWD16_001598"
     CDS             complement(5053..7764)
                     /locus_tag="IWD16_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9088660.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="IWD16_001599"
     CDS             complement(7774..8475)
                     /locus_tag="IWD16_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9088661.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="IWD16_001600"
     CDS             8661..9260
                     /locus_tag="IWD16_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9088662.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="IWD16_001601"
     CDS             9341..10354
                     /locus_tag="IWD16_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9088663.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="IWD16_001602"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="IWD16_001602"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP9088664.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="IWD16_001603"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="IWD16_001603"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP9088665.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="IWD16_001604"
     CDS             11802..13175
                     /locus_tag="IWD16_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9088666.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
BASE COUNT         3771 a         2632 c         2458 g         4418 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttgg
//
LOCUS       DACDMM010000072        13158 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDMM010000072 DACDMM010000000
VERSION     DACDMM010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.072"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            12..84
                     /locus_tag="IWD16_001605"
     tRNA            12..84
                     /locus_tag="IWD16_001605"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="IWD16_001606"
     tRNA            88..160
                     /locus_tag="IWD16_001606"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="IWD16_001607"
     tRNA            168..249
                     /locus_tag="IWD16_001607"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="IWD16_001608"
     tRNA            267..339
                     /locus_tag="IWD16_001608"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="IWD16_001609"
     tRNA            351..422
                     /locus_tag="IWD16_001609"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="IWD16_001610"
     tRNA            439..524
                     /locus_tag="IWD16_001610"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="IWD16_001611"
     tRNA            535..608
                     /locus_tag="IWD16_001611"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="IWD16_001612"
     tRNA            618..691
                     /locus_tag="IWD16_001612"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="IWD16_001613"
     tRNA            711..783
                     /locus_tag="IWD16_001613"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="IWD16_001614"
     tRNA            811..884
                     /locus_tag="IWD16_001614"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="IWD16_001615"
     tRNA            898..971
                     /locus_tag="IWD16_001615"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="IWD16_001616"
     tRNA            995..1084
                     /locus_tag="IWD16_001616"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="IWD16_001617"
     tRNA            1097..1170
                     /locus_tag="IWD16_001617"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="IWD16_001618"
     tRNA            1189..1264
                     /locus_tag="IWD16_001618"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="IWD16_001619"
     tRNA            1266..1336
                     /locus_tag="IWD16_001619"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="IWD16_001620"
     tRNA            1350..1423
                     /locus_tag="IWD16_001620"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="IWD16_001621"
     tRNA            1448..1535
                     /locus_tag="IWD16_001621"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="IWD16_001622"
     tRNA            1557..1628
                     /locus_tag="IWD16_001622"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="IWD16_001623"
     tRNA            1651..1740
                     /locus_tag="IWD16_001623"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="IWD16_001624"
     tRNA            1753..1826
                     /locus_tag="IWD16_001624"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="IWD16_001625"
     tRNA            1845..1917
                     /locus_tag="IWD16_001625"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="IWD16_001626"
     tRNA            1921..1993
                     /locus_tag="IWD16_001626"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="IWD16_001627"
     tRNA            1998..2068
                     /locus_tag="IWD16_001627"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="IWD16_001628"
     tRNA            2081..2154
                     /locus_tag="IWD16_001628"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="IWD16_001629"
     tRNA            2166..2237
                     /locus_tag="IWD16_001629"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     gene            2503..3696
                     /locus_tag="IWD16_001630"
     CDS             2503..3696
                     /locus_tag="IWD16_001630"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP9088667.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="IWD16_001631"
     CDS             3826..5298
                     /locus_tag="IWD16_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9088668.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="IWD16_001632"
     CDS             5381..6169
                     /locus_tag="IWD16_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP9088669.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="IWD16_001633"
     CDS             complement(6236..6904)
                     /locus_tag="IWD16_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9088670.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="IWD16_001634"
     CDS             7019..8731
                     /locus_tag="IWD16_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9088671.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="IWD16_001635"
     CDS             8734..10503
                     /locus_tag="IWD16_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9088672.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="IWD16_001636"
     CDS             complement(10708..11274)
                     /locus_tag="IWD16_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9088673.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="IWD16_001637"
     CDS             complement(11274..12230)
                     /locus_tag="IWD16_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP9088674.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="IWD16_001638"
     CDS             complement(12230..12886)
                     /locus_tag="IWD16_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9088675.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
BASE COUNT         3834 a         2461 c         2932 g         3931 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatg
//
LOCUS       DACDMM010000073        13119 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDMM010000073 DACDMM010000000
VERSION     DACDMM010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.073"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(147..773)
                     /locus_tag="IWD16_001639"
     CDS             complement(147..773)
                     /locus_tag="IWD16_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088676.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1068..1403)
                     /locus_tag="IWD16_001640"
     CDS             complement(1068..1403)
                     /locus_tag="IWD16_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9088677.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1390..1674)
                     /locus_tag="IWD16_001641"
     CDS             complement(1390..1674)
                     /locus_tag="IWD16_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9088678.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1730..3253)
                     /locus_tag="IWD16_001642"
     CDS             complement(1730..3253)
                     /locus_tag="IWD16_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9088679.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3246..4421)
                     /locus_tag="IWD16_001643"
     CDS             complement(3246..4421)
                     /locus_tag="IWD16_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9088680.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4425..4610)
                     /locus_tag="IWD16_001644"
     CDS             complement(4425..4610)
                     /locus_tag="IWD16_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088681.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4576..6270)
                     /locus_tag="IWD16_001645"
     CDS             complement(4576..6270)
                     /locus_tag="IWD16_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9088682.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6267..6740)
                     /locus_tag="IWD16_001646"
     CDS             complement(6267..6740)
                     /locus_tag="IWD16_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP9088683.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6809..6964)
                     /locus_tag="IWD16_001647"
     CDS             complement(6809..6964)
                     /locus_tag="IWD16_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP9088684.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7098..7478)
                     /locus_tag="IWD16_001648"
     CDS             complement(7098..7478)
                     /locus_tag="IWD16_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9088685.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7482..7697)
                     /locus_tag="IWD16_001649"
     CDS             complement(7482..7697)
                     /locus_tag="IWD16_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088686.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7700..8107)
                     /locus_tag="IWD16_001650"
     CDS             complement(7700..8107)
                     /locus_tag="IWD16_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP9088687.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8373..9848)
                     /locus_tag="IWD16_001651"
     CDS             complement(8373..9848)
                     /locus_tag="IWD16_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP9088688.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9838..10686)
                     /locus_tag="IWD16_001652"
     CDS             complement(9838..10686)
                     /locus_tag="IWD16_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP9088689.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10723..11085)
                     /locus_tag="IWD16_001653"
     CDS             complement(10723..11085)
                     /locus_tag="IWD16_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088690.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11385..12009)
                     /locus_tag="IWD16_001654"
                     /pseudo
     CDS             complement(11385..12009)
                     /locus_tag="IWD16_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12006..12317)
                     /locus_tag="IWD16_001655"
     CDS             complement(12006..12317)
                     /locus_tag="IWD16_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088691.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12361..12654)
                     /locus_tag="IWD16_001656"
     CDS             complement(12361..12654)
                     /locus_tag="IWD16_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088692.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12848..>13119
                     /locus_tag="IWD16_001657"
     CDS             12848..>13119
                     /locus_tag="IWD16_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9088693.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHM"
BASE COUNT         3634 a         2743 c         2065 g         4677 t
ORIGIN      
        1 gaaacccata tcaaagaact ataaaggtta tcaaaacagc gtttatgact aaatgaatta
       61 gagttatcta tcactatttt tcaagcgatt taaaccaaat ggcatacaat atggcataca
      121 aaaacttcaa taagccgtgg gggtttttaa atttcttctt gttctttaaa atcaatgagt
      181 ggagctacat aatttgaatc cactttttca aacgtcactt tttgcatcga cgaatgtttt
      241 ctttcccaag ataacgtaaa tagatttgac tctggactta taaagtctac ttcaccttct
      301 agtattactt tcttatgcaa atattcctct aataaatata gacttgctaa agcatacaac
      361 acattttcca aatttgcttc cttataattt gaagaacgac catgttttac tctgttatat
      421 attttccacc aatctggtgt atcttcaggc cacccatctc caaaaggttt tagtgaagtc
      481 aatgttaaag aattcataac cctaacacca ttatccaaac acattaattc gctagattta
      541 ataacagctt taaaatcagt tatgctttta ttgctactgc tatcaaatcc gcaaagttct
      601 ttgaaaataa tctcacactc cgaaccaatc gctagtagta gtttttgata ttcaatagag
      661 aatgttttga aattattctc atcgagcatc acaaaatctg tggtattaat aaactcattt
      721 tctaaatgta gataatactt ccaataaaaa tttgtaaact ctgctttgtc cattaaatca
      781 cctcttttga attataacaa aaaaaacacg tccacaacag acgtgttctt tgatttctac
      841 acaatcagct tgccaaaacc atccaatcag tcctagcatg cctgtattaa aattgaacgt
      901 tcatcatcta tcatctttct tagtccagca tgtttgcaac cacacctaca attttcagtc
      961 caacaataga tttcgactag ctaagtcttg caaaattcag gcagctaacc cttcatttac
     1021 taccttaatt atactaaata cccacaacgt ttacaagtgt aatcattcta agaaatctgt
     1081 ttagctatga tcgttgtata atctttaaac gccgttcctt cattgattga aataatttta
     1141 taactcttgt ttttccaagc tactttcata tcgtttgtta gttcataatc ttgatcgtat
     1201 cgaataataa acgtcaacgt tccttcaaga atggtaccta cactagcaag cacgtctttt
     1261 agcatttgtg aacgcacatt agcccaacat tcaaatacag tttcttcact atccaccatc
     1321 tgcccatctt catcttttac ccgttttgtc tttttaaact tgactctctc agtcaattca
     1381 tttacattct taatgattgg cataataagc acctctcatt tgttgtacta atgcttgtac
     1441 cgttactggt atccgttcgc tgctggcttc ttgacggttc aaataccaat gttgagtaag
     1501 taatgacacc gccaaatcaa attgctgata attatccata tctttatccg ttgcgctact
     1561 atcaatagca ctttttatat attcgcctgc tgtatcaatc aattgttgaa taaagtcatc
     1621 atcaatagtg tgatcgatcc gcatactttt ttttatgcta tccaaaatta ccattttatc
     1681 tctcctaatg aaaaaaaggc agtgggataa accgccgccc aatatttttc tatacttttg
     1741 gtgttacaac tggcgtaaac tcaatataca ctgaagcatt ttgatcaatt ttcttataat
     1801 cgttgcgaac gatcactgca agtccttgtg agtagtaatc gaatttttcc cattgagtcg
     1861 ttacttgatt tctacgagca acaaagatag attgtgcaat atcccccata atcataggga
     1921 atgttccagc tttaggattg gcaaacaatg tatctgcaat caatactact ggcataccga
     1981 ataatgattt accactaggt gctgtcacat cgggttgtaa aatgtaacgt ccatctgaat
     2041 ctttcaatgt atctaggtgg taatatccgc tttggtttag aattaccatt ttatttaatg
     2101 ctgggtctaa tgccacattg tatagttgtt tcaaatcgtc caacgtagca gccgttttct
     2161 tagtgaatgt ttttaataaa tccatgatat gcttattgtc agtgttttct accaatttcg
     2221 ctaactgatc tttgacctct tgtacaatat tcactgctga atcttccaca acctcatttg
     2281 ataaggcaat cttaccagca cgagtttcta ctttataatc aactgaagta aacatttcag
     2341 cgtcaatatc accaatttca gcaagttcag cttttgttgc taacactgct tgttggttag
     2401 ttgctactgg atatttacct tgtccatttg atactgtttt tactgttgca tattgagcta
     2461 agttataatt tgaacgtttc aaatcaaaga cttcaccgat tacttcttcg ggaacaactg
     2521 ccgctgcatt tacagtagtg actccatcac gcacttcgcc ttgtgaacgg atatattctt
     2581 catacccacg caattctgtt ttttcgttat cgattaaagt tttttccatg ttattactct
     2641 ccttttgtgc tttagaagtg gattctaagc tattgtttgt ttgttttgct tggttgttac
     2701 tcataaacga ttcgtaagaa cgcttattta cttggacatt tgatgaatca taagcgggaa
     2761 cggtcacgac actaatttca ttaagtgctt tgattttatc tattgatcgt tcaattgtgc
     2821 catcttcttt tttgtcgaat gaatctaatc caagcacgaa cccaaaggac atagaatcaa
     2881 caaccccttt tgagatattc tcatacacat catttgaata ggtcgtgtcg gttagctctg
     2941 cttcaaaatg taatcctgta tcatcgacat tcaatttcaa cgtacctgct ttgacactag
     3001 ctagtggctt tgaataatcg tgtccatata ataagaacac atttgataaa tccacctctt
     3061 ttaatgcttc gggtgtaatc acttctacaa agccgcctaa atcttttgac ggttgcccga
     3121 atttcaacgc atagcctgaa atagttttgc cgtctgttgg ttgtttttca ttttctttct
     3181 ctactttggg agaatcggct gttagctcag cttcttctgt cagccgtttt tccttttctt
     3241 cttcattatt caactggtgt aactccttcc tgttctttta attgatattt ctccaacgta
     3301 tctaaatacg taaagttcaa acttgctagt aaacgatcgc caccatccat ggggggtagc
     3361 cccattttcg accgcccctc attaatcgtg agaagtgacc ctttgacctg atccaataca
     3421 ttttttactt ttgtttcagg atcagtctct agtaactgat cggaattaaa gcggatattg
     3481 gtttcaagtt tcgtatctaa ttcactcaca aacacattaa agtaatgaat cagcgtactt
     3541 tggatatact ggagattact ttgaacggtg ctagaatgtt cattttcaac gcctaaacgc
     3601 tcaatcggta caccaaacgc cttggcaatt tgttttgtgt tccaatcatt ggaattgata
     3661 agcttcaaaa catcggtgtt tatttctaat gttttgtagt ccattgtttc atcaagaatg
     3721 atcgttctaa gagcattatc tccactactt gaaccgttgg cttcttcaaa cttttcacga
     3781 attgcgttct ttgctgatcc gtctaaatct gacttatgaa ccttcaaaat acctgaccca
     3841 ctgacaccac gagtaaagaa attgtgcaat gtgcggttgc cagcttgttg aattttcagt
     3901 tcatcacgta acgcataaag tggtggtaat cctgttaaac catcttgggt gaaatatttg
     3961 aagtgcaaaa tatcgctaga cttcacacgt ctttttttat caccaatctg ataagacaac
     4021 gtaccattat ctaattgagt aaccgttaca cttgagttag gtaataagtg aatttcttct
     4081 accttttcac cgttgcgttt aatctctgca aagctattac cgtttagcaa catattgacc
     4141 gccaaagcaa actttaaatg ccaaccatcc atttctgaat taggtttctc gtttagtaac
     4201 ttcaccagtt cattatcagc ttgtggcata ttgtttttga ccaattgaat cggacttgaa
     4261 gcaatatcac tagcaataat tcgaacggct gcaaacacat cactatttct taatgcacga
     4321 acgcttgtaa agctggtggt gtaatcatcc gattggattg ataccacatg ttgtaggaaa
     4381 ggatctcccg ttgtattttg tctcgcttta aaaaatgcca tcttttaacc tcccttctgt
     4441 tctgtattta gcatgaccgc taccccaata agcactatgc ccagcgctaa caagccatag
     4501 tagacatttg ttagtaaggt gatagcaacc aacacacaca ccaatccaag aagtaaaaga
     4561 acggtatgaa tgtaattaga aagaaaattc ttcacttgta taaaattcat tgtctgcttt
     4621 cgctccttcc gttgtggtga agtaatccat tgcaaacaca taggcattga ttaatgcagc
     4681 tattggatca attttgttac tgttctttga tttattaatc tgaatgccgt tgttatccgt
     4741 ttttaaaata gcgttgttca ctgcatgggt gagaatcgta tttttattgt gaataacgtt
     4801 gccttcataa acttgttctc taaaagttcg ggtcggaacg tttagagtaa tcgttccttg
     4861 tcttacttct aacattgggt agttggcttt ttcagcttta ctgataagcg aattagcgtt
     4921 atacgggtca taacaaatcc ccatacattc caaatcattt tcttgaatca aatcttgaat
     4981 aaactgaaat acttggtcat agtccacgat tccactttct aattgagtaa tggaacactc
     5041 accagctttt tctagttctc gataaggcaa accgtcgcgc ttttctttgt cttgaagccc
     5101 atatttggtg gctacaaagc tatgtgaatc acaataaagt tcgccgttat ctagcggtac
     5161 gatccatgaa acgctagtta aatcatctgt tttagataaa tccactccga tataaacggg
     5221 cttgcctgta atatcttgtg gttctacttc ggttgcctgc cagtcttcag caatcatata
     5281 gctgtcctca ctcgcttgtc tccataaatt gaagtttttc accaatacag aattaaggtt
     5341 attttgtttc aaagcaaggt tcacatcatc ttgaatggtt ggaatcatta cttttttgat
     5401 ttcttcactt tcaaaaatcg gattggcttt gatccatttc tcttgatcgt gaatttcttc
     5461 gggatcgtcc aattcccata tcgctataaa gtacctgtcg gcttgttctt cgccttttag
     5521 cacacgatct aacaataagt attcctcgta cataggaacg tttaaatcaa gcccagcggt
     5581 actaataaca cacaataagc cgttcttttg ttgggtcatt cctgacttaa tgacgttgta
     5641 cgttttacga gtttttgctt cgtgccactc gtcaagaatt gcggttgttg gtgcaaaccc
     5701 atctagcgtg ctggtttcac tagccagtgc catagcgaac gaattagagg gcaagtgctg
     5761 aatcttagag ttcataattt tcagttgtgg tcttaaaaac ttactagact tgaccacgtt
     5821 tctaagtgaa ttagacagca tatcatagcc caatttagct tgtttcaaag cgttacttac
     5881 aaacaatact tgccttgctt ctgctggttc ttgttccata atcaacgcat tggcagccat
     5941 gccacttgcg atatacgttt tcccattctt acggctcata ctggtaaacg ctcgattgaa
     6001 tcgcctgtaa ttcccttctt tagtacgcca gccataaaga ctgcctagaa tgaatttttg
     6061 aaatcctaac atcttgattg ctttaccgtc cgtagatggt agcaactcaa tgaatttaat
     6121 cgctttggtg gcttgttctt catcgaacac ataagggaag ctatctgact tagaacgctc
     6181 caaatcgctt atatgacgct cacacgcttg gataattttc ttgggtgcaa tcagcttacc
     6241 tgataggact ttctcaatgt aatcattcat gaattcatca actctttgaa cgggtcttcg
     6301 ggttcatcat ttttaacctt gttcaaagct agttttgatc gtgattctaa ggtcatacct
     6361 agtgaggtgg cttgtttttt caaatcactc atagcttgtg attgaacccg aaccgctgga
     6421 ttggctttct ttactccgtt ttcgcatgtg ataaacgtac ctgattttct tatctcggct
     6481 tcggctgttt tcattcgagc aaaggctaaa caataggcta ccaatgaacc ataatctgct
     6541 tcactcaatg gataatcttt ttttaatgtt ggtacgatac gattccattc acttctagcg
     6601 ctcgctgcca tccagtcagg gggcgtgata tttattaatg gttgctgctt gaacaattct
     6661 tctctagcgt ccacacggat tgctttttct tcacttgata tattgccttt tgtatcgtgt
     6721 aatattttag ggtgtcccat gttgtcctcc tttcagacaa aagaaaaagg aacgtaagca
     6781 attaagctca cattccttcg tttttcgatc agaatttttc tttatctaat ttatctatat
     6841 aaatgatttt tccgttctgc atttgaatgg tcacttgacc aaaatgaggt aactcaatgg
     6901 tgcgctgttt ccctgtttta tcagtaataa caattgtatt tttcaataaa tctcctactt
     6961 tcattatata ctttttcact tgtgattaca agtgtaatcg ttatttgtaa ttacccttat
     7021 gccttttcac aacggttatt tgtaccaaaa tgagtaggca tcgattgtca gtaagtttca
     7081 cactatgccc ccttaactca aagggtactc ttttctcgtc tttgtctttc attggctgtc
     7141 ttggaattat ggtgtgcatg gcataaagaa cgaaaattag tttcatctaa ccgtctattc
     7201 caatcatctt ttagttcaat tttgtgatct acaacatcag ctttccttat caagccttct
     7261 tctaggcaat cctcacatac tggatgattt aaccgataca agtatgaagc ctttcgccat
     7321 gttctacttt gatagaattg aaaatattta ccttcgcttt gctttctttc ttcacgctta
     7381 attgccgttt gctttgcttt atgcttccca caatacttct gcctataatc aatcaatgtt
     7441 ttacatccag catgattaca ttgtttttta actgccatag attaccaacc aaagcgatct
     7501 cgttcgtcag aatcatcttg ttcccatttt gatttgtatt caatataagg cgaaactatc
     7561 ttcaagcacg ttccgtcttt aaaattaact ttagtgattt tatcttcctc aaaactaata
     7621 aagtctacat cttcttgagt gtttgaatac tcaatgtgtt tctcaccatc gaagtatcta
     7681 atactttcca tatgcataat tattctacct ccaataaatt agttatcaat tcatcatctg
     7741 ttagtagcaa agccaagaac ttatcatgga tgtttaaaaa caaattatcc tcattcgatg
     7801 taagctctcg ttgtctcgct cgatcatata ggttaagcag acaattaaat tcatccatta
     7861 gctcacctgt atcatcatag ttgccactat ctaccaatga tcccactact agcgattgac
     7921 accacgctac tgactcctgc agccaatcag gttcttcttc cattatcttt aatacttctg
     7981 gatcaaatgt ggaataatct tctgttacca atcccacact aaatgccagc gtgttcatta
     8041 gaaaccgatc catttcatct gtatattgaa agttcggtgc tttgtttgtt gaaataatat
     8101 tgagcattta ctttcctcct tgattcgtaa cacgtggtaa cacctacgta acaggtaccc
     8161 gttaccactc aatcccaacg gggacaaggg ttctagcgtt ttggtaacag gtaacgggta
     8221 aaatccagta acattattcc ccagcatatt agctaactat ttcaaaagtt ttaaggaaat
     8281 aaggtgttac cccgttacca aattgctgta tcccttgggg cagtaagggt aaactagtaa
     8341 cgggctaggt gttactgacc cgttactacc cgttactttt tgtactttcc agctcgattt
     8401 ttattttcat aggcatttga cttattgtaa aatcctcgtc gtttatttct ttttggcagt
     8461 tgcttccgtt cccaaccagg aatactgcta ataatcaagg caatcttttt agtttctgca
     8521 tctgatcgac cattcaataa atcctttgct tgtttatcaa aaacagcttc caaaatatca
     8581 gcggtgagta cgctatcaac taaataaaac tcttgttgca tagtagctcc attatagaaa
     8641 tgctgtaaat ttttctcatt acatttactg tcaggatagt tctgataata agaacgtctc
     8701 gcccacgctg gagcttcttc ccatttatac gggatttcca tttccaaata tttagtgatt
     8761 gcttctttaa ttggattctc catcttggca tcctcttgat agtcttttgc aatttctaag
     8821 acttctttat cttcatcggg actaaaatag attcgttgtc ctttatcaaa aagcaccttt
     8881 gcttcagcta acacttgaag aaaatattca tcctttgttt taaacggatt catctttggc
     8941 ttattcttac aaggcaaagg ataaaaacgg cggtttcctg tatcatcttt caaatactgc
     9001 ccatcattcg acgttccaat aaatacacat tgccttgccc aagctatgac attccgacca
     9061 taaggaggtc taattttatc gatcttggca ctgatgaagt tttttatctg ttcaatttta
     9121 gttttgttca ttgaagataa ttctccaagt tcaataatta cattgccttg aagctgcata
     9181 taatcatctt tcttttcacc tagcgaatct aatgaatcgg tgaaaaaatc atccgtgtaa
     9241 agtaatgctg gaacggtact cttaccaatc ccttgcggtc catccaaaac tggaacgatc
     9301 tcaaacttta ttcctggttt atagatacgt gcgactgatc cgactatcca acgttttgtt
     9361 acttctcgtg tgtaaaggtt gtcgtctacc cctaaaaggt caataaacag tgtttccagt
     9421 ctaggctgtt gatcccattt aactttttcg atacgttctt ttactgggtt gaaaacattc
     9481 tttctagcca catttactac tgctttgtgt attttgttgt catcaaaaac tacattgtag
     9541 ttttcttcaa tatatgccgt taaggcatcc tcaaaactgt cttcccattc tccagcggta
     9601 cgattgatcc aaggagaatc ttttaatagg tgaatgtatc cgctaaattc gttataagca
     9661 attgttccag ctaaattgct atcattggtt aaaatcaatt ctgcattcac aacactgtta
     9721 cttttaatct gatcgtcttt attgcatctt aatcgtgaca tccaggtttt caattcaatg
     9781 atattgtctt cacgctcgct ggcttcggga aagtctaaaa tactatctaa tttatgctta
     9841 tgagccatga gctttccccc tcaaatcttt tttaatcatc gatcgaataa tttgattcac
     9901 ttctttatct tttagcggtg gttcattgta cgcatttgct gtataaatca attctgctaa
     9961 agtttgatta tctacaccgt gccatagtaa ccaaccgcat aacttagtta aatgatcgtt
    10021 tctactacct tcgccagcgc cttgaacaat catatctaat ttttttgctg tccctgtttt
    10081 tgctacgggt cggtattgag tctttatttc ttgcggtttt tttagcagta ggttcactag
    10141 ccaatcggga gctggtgcaa tattatttag agatacgttt tcaataggtt catatgctct
    10201 cccaacactt cttgaaataa tcatagttgg cgctattgga actcctttag tgataatatc
    10261 aatcccgctt aaacttttac ctttatcatc acgcccgaag atatcagcaa atgcacctgt
    10321 ttcttgttgg ataattacac catcaggaat tttaaagaag aaatggattc ctttacctgg
    10381 tgtgatttcc gtataagtag aaggaaatgg atcatacatt gaagcaagtt ttttataatt
    10441 ctcaacgccg tttcgtgttt cagaatgttg atctaggtca actaccacca tttgatgatc
    10501 ttcaagcatt aacccaatat ttctagttgc atcttccgtc caccattcat taatttgatt
    10561 agaatcgtta gttgcatcat tccaaccact agagcctttt attggaattt tagttccagg
    10621 agataaggga taaattgacc atcccgactt ttgtaattcc gttgctgctt gataattatt
    10681 tgccaataaa cctctcctcc tttttcaaaa tactggtata aatcattcag acacctccat
    10741 gagcttcaac tgctcaagtt cgtatttcaa cccatcccga acaaattcag caccttttaa
    10801 ttgattctga tactttaccc atgtattaaa tgttttcacc cttgcttctt gcatatcagt
    10861 aatgtttaac catgcggtta aaatctgatt ttttagctcg ttgatttctc gacaaattat
    10921 tttgtgttct gcatgttttt tgagtacata caaaactgtt agtttttcat tgaatgaatt
    10981 tccaaagtag gttttcccga tattattttc tagttcagcg atcccttgat taatttcctc
    11041 taattcaagg ctatcgagtt ggtttaatag ttttgttttg tacatctact tatgccctcc
    11101 agttcttaat tgatagtcgt actcgtcata agcagtgaag agatataacg ctaatcctgc
    11161 aaagatagct atttttgcaa ttaatggtaa taagccgaaa attccgccga ttaaaatgct
    11221 tcctataatt aagtttcgtt tcataatggt tccctccgat tttttaatat ggtataatcg
    11281 gaacaaagaa tatcgggttt tatcccaatt aagttgcctt tatcctgtcc gccaagactg
    11341 ttatggataa ggctttttct ttgttctcga tcatgttaat gctcctaagt tgtctgcaat
    11401 tcagcagaac gattataaat tttctcactc actgaaaact gcaacccata tttttcttta
    11461 acagtcatta attctactgt ctcatctaaa ataggttcac gatcaaccaa catttcagga
    11521 gtcatatctt tcttttttac cattttggaa tgaccataaa tagttgatac agctttattg
    11581 gctatcacat tcgctttaat gtagtctact ggttttggtt cagtaatagc gtggcttaat
    11641 ttagtcattg cttctttttg gtgttctttg tctagcatgc ggaatacttg aaagccttca
    11701 agtcctgtgg actggcgtag ttctttgatg atgtcgaaaa tccagtcttg aaatgcctcg
    11761 gcttcttttt tacggctttt gaatgccatg cggtaaatat tttttttcgt tgatgatgtt
    11821 tacatcttgt gttctgccca atgaatcgat gaccttacta gtagtaaccc catctttagg
    11881 caaagagtga atggctctag taacctgttt aagacctaac gcttccgcaa catctttcgc
    11941 cactgcccac cattcatcat taatatcgac aaatcttata atgtgtccgt tccaaatttc
    12001 agttttcatt caacgtcctc cagttcaagc cctagcaagc tcgctaacaa ctcaacttgc
    12061 tgatacaaaa tttcttgata ctcttcaata gaaagtcgtt ctttaaactc tgcttgtact
    12121 aaatcgttca agccacgcaa tactccgctt accgttggtt cttgtggtag caatttttga
    12181 tagtccattt gttcgtacaa acattcaaac gtttcttcta gcgctttgcc ttccaagttt
    12241 gctaattctg ccgctgtgtc ttctgtaaga attgttgata atactttttc gattgcttta
    12301 ttttgtgtcg attccattat tatttcctcc tattaatccc tttgccccca cggcttgcgt
    12361 ttatttttgg tattcttcca taaagcgatc acattcatct tgtgaaattc ttacgactcc
    12421 atcaactata atcaacttta aaccgttagg aacatatttc tgcatcatag tattgtatga
    12481 aacgttcata tacttagcag cttgtgcctt gttcatatat tttggaaact ggtacttttt
    12541 ttctaataat gaaagtaacg ttccttgagt ttgctctact tgttgaacaa ctcgatcgtc
    12601 aataatttta aaagctggat catcaagcgt gagtttgtca atttttaatt ccatatttat
    12661 cccccctcgt tagcatacat ttctatacaa agattttaaa aaaatgcaag aggctcttga
    12721 atacatttct atacctctta taaactcaat ataccatcat accttttcat ttgcaagaat
    12781 tttaacatac ttttttgaac ttttatgttt cacacatatt taaatcgtga tatagtagag
    12841 aaaatatatg aatggtggtg aaaatgtggc tagaaaaaaa taccccgaag cagtggagac
    12901 aggacaaaga attagactgg ctagaattga aaagggactc tcaatggaac aacttggagg
    12961 taaattatcc ccaccggcca gtaaaggtgc cgttagtaat tgggaaaatg gctataatct
    13021 tcccaataac accaggctta aacaactttc aaaggtatta gatgtatcca ctacctatct
    13081 attaaacgga tcttatacat taagagatat gcatatgat
//
LOCUS       DACDMM010000074        13006 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDMM010000074 DACDMM010000000
VERSION     DACDMM010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.074"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            66..767
                     /locus_tag="IWD16_001658"
     CDS             66..767
                     /locus_tag="IWD16_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP9088694.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="IWD16_001659"
     CDS             764..1885
                     /locus_tag="IWD16_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP9088695.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="IWD16_001660"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="IWD16_001660"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP9088696.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="IWD16_001661"
     CDS             3264..4172
                     /locus_tag="IWD16_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP9088697.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="IWD16_001662"
     CDS             4207..4479
                     /locus_tag="IWD16_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP9088698.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="IWD16_001663"
     CDS             4490..5536
                     /locus_tag="IWD16_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP9088699.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="IWD16_001664"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="IWD16_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP9088700.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9218)
                     /locus_tag="IWD16_001665"
     CDS             complement(8547..9218)
                     /locus_tag="IWD16_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088701.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9211..9705)
                     /locus_tag="IWD16_001666"
     CDS             complement(9211..9705)
                     /locus_tag="IWD16_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9088702.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="IWD16_001667"
     CDS             complement(9725..10945)
                     /locus_tag="IWD16_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9088703.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="IWD16_001668"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="IWD16_001668"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP9088704.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         4258 a         2378 c         2727 g         3643 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc caatagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gttttt
//
LOCUS       DACDMM010000075        12101 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDMM010000075 DACDMM010000000
VERSION     DACDMM010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.076"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(151..531)
                     /locus_tag="IWD16_001669"
     CDS             complement(151..531)
                     /locus_tag="IWD16_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP9088705.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            613..1788
                     /locus_tag="IWD16_001670"
     CDS             613..1788
                     /locus_tag="IWD16_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9088706.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(1865..2449)
                     /locus_tag="IWD16_001671"
     CDS             complement(1865..2449)
                     /locus_tag="IWD16_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9088707.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            2650..3648
                     /locus_tag="IWD16_001672"
     CDS             2650..3648
                     /locus_tag="IWD16_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9088708.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            3760..4269
                     /locus_tag="IWD16_001673"
     CDS             3760..4269
                     /locus_tag="IWD16_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP9088709.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4299..4958)
                     /locus_tag="IWD16_001674"
     CDS             complement(4299..4958)
                     /locus_tag="IWD16_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP9088710.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(4960..5724)
                     /locus_tag="IWD16_001675"
     CDS             complement(4960..5724)
                     /locus_tag="IWD16_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP9088711.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(5732..6409)
                     /locus_tag="IWD16_001676"
     CDS             complement(5732..6409)
                     /locus_tag="IWD16_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP9088712.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(6446..7831)
                     /locus_tag="IWD16_001677"
     CDS             complement(6446..7831)
                     /locus_tag="IWD16_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9088713.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8041..8125)
                     /gene="ffs"
                     /locus_tag="IWD16_001678"
     ncRNA           complement(8041..8125)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWD16_001678"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8148..8663)
                     /gene="tadA"
                     /locus_tag="IWD16_001679"
     CDS             complement(8148..8663)
                     /gene="tadA"
                     /locus_tag="IWD16_001679"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP9088714.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            8770..9627
                     /locus_tag="IWD16_001680"
     CDS             8770..9627
                     /locus_tag="IWD16_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP9088715.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(9708..10043)
                     /locus_tag="IWD16_001681"
     CDS             complement(9708..10043)
                     /locus_tag="IWD16_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088716.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10110..11480)
                     /locus_tag="IWD16_001682"
     CDS             complement(10110..11480)
                     /locus_tag="IWD16_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP9088717.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(11558..12094)
                     /locus_tag="IWD16_001683"
     CDS             complement(11558..12094)
                     /locus_tag="IWD16_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9088718.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         3578 a         2464 c         2054 g         4005 t
ORIGIN      
        1 attgaatgga ccaaaaattt tttagtacca tcctccaaat gcaacataat tgtaccagca
       61 actcttttgc ttgccaaaat ttccacctcg atcaacttgt tctatggctt acataaaaat
      121 tctagcacat tttcagaaaa aatacacttt ttatttctta ttttctttcc gttctagttc
      181 agataatgct tcttctatcc aaataggtaa ctgctccgca atagtggtaa atccgatctt
      241 acgattttta ttcgtgaaat acctttttct gcgatatgga acagctggat ttgttttttc
      301 taacgttctt aaagataaac tgattttttt ggaatattca tcaatgtcga tgatctgaac
      361 tgttactggc tgtccgactt ttaacaatga atggatattt ttcgtataac cggattgtac
      421 ttctgaaaca tgaataagtc cttgcgtctc gttatcaaga gaaacgaatg cgccataagg
      481 ctgtatccct gtcacttttc cttcaattat ttgtccaatt ttataagtca tctacttccc
      541 tctcttcgca tttatcatta tactgtaaat agctcaaaaa aagaagcgac gaaaatgact
      601 ggaggtctta agatgatcga atttgataaa cagatttcaa gaaaagatac agaaagtgta
      661 aaatgggacg caattgctga gacatatcaa atggatgact tacttccttt gtgggtagca
      721 gatatggact tcttgtctcc cggtggggta gctaaagcgt ttagtgaata catcaaacat
      781 ggaatctttg gttatgccac tctgtctgat aaactatatc aggctatcat tcattgggaa
      841 cgccaacacc atgctgtgga gttgacgaaa gaaaatatcg tttttacaag tggtgtgtta
      901 accagtttag ctgcagcagt acaaactttt acaaaaccag gcgattctat tttgattcat
      961 gacccagttt atcctccatt ttcctctatt atagaaacaa atcaacgtca catcgttcga
     1021 agctctttgc tagaaaaaaa taatcatttc atcatggact tgtctgacat ggagaaaaaa
     1081 atcgaagaaa acaatgtaaa agcaatgatt ttatgcaatc ctcacaatcc tggagggcgt
     1141 gtttggagca aagaagaatt gaaacagtta agcgaattgt gtttaaaaca tcaagtgatt
     1201 ctgtttagtg acgagatcca tcaagattta actttattcg atcatacatt tacttccatg
     1261 ctaacgattg atcctaattt agatgatcta ctagttgctt ttacttccgc aaccaagact
     1321 ttcaatctag ctgcaatcaa aaattcaatg gtatttatca aaaatccaga gatgaaagct
     1381 gattttgaaa aacatttagt gatgaaccaa cagcacgaaa tcaacacttt tggactgatt
     1441 ggaacccaag cggcttatga gacgggtgga gaatggttaa gccaactgat tccttatcta
     1501 gaaaaaaatg ttcaaacagt taatcagttt tttgaacagt atcttccgaa agtgagaata
     1561 atgaaccctg aaggaactta cttaatgtgg ttagatttta ccgcctatat ttcagacgac
     1621 caagaattgg agaacactct tgttcggaaa ggaaaagtcg tattaaatcc tggcatcacg
     1681 tttggtcctt ccggtcatgg gcatatgcgc ttgaatattg cttgtccaga agaaacttta
     1741 ctggaaggac ttaatcgaat caagtatgct ttagagacaa tcgattaaat catttattca
     1801 aaataaaagg acgagacctt actaggtttc gtcctttttc ataattactc cgtaccatac
     1861 caaactattc tgaaatttca attgtttcaa tcactacatc atgtacaggt cggtcttgtg
     1921 gaccacgttg tacatttgcg atttcatcta ctgtgtccat cccttctagt acatgtccaa
     1981 agactgtatg tcggaaatct agccaaggag ttccgccacc tttatacgct tcaatgattt
     2041 ctgatgggaa tccagcacct tctagttgtc caagcatatt agctggcaca tttgtgttat
     2101 ttacaataaa gaattggctt ccgtttgtgt ttggacctga atttgccata gataaagctc
     2161 cgcgaaggtt gaacagttca cgactgaatt catcttcaaa actttcgcca taaatacttt
     2221 cgccacccat tcctgttcca gttggatcgc cgccttggat catgaaatca ggaataacgc
     2281 ggtgaaaaat cacgccatca taataacctt ttttggaaag ttcaatgaaa ttttgaaccg
     2341 tcttcggtgc tagttcaggg aataattgaa cagtaatgtc accacggttt gttttgataa
     2401 cagcttttgg tcctttttca ctttttaaat ctagttgtgg gaatgccata ttgtttcctc
     2461 ctaatatttg catacttcat aataataata acataatacc taacctctcg taaaaatact
     2521 agatttgttt tcttttattt ttcatcacta gatacattct ataacttacc gtttcgattt
     2581 cgaattcaaa catttttgag aagcattctc aattaaattc tgctatagtg tgaaaaggga
     2641 ggaattttta tggaaatcta tgacattaca atcatcggtg caggtcctgt agggatgttt
     2701 gccgcttttt acgcaggaat gcgccaagct aaaacaaaaa tcattgacag tctacctcaa
     2761 cttggtggtc aattagcaac tttgtatccc gaaaaatata tttacgatat tcctgggtat
     2821 ccagcaataa aagcaagtga attaatcgat caattagaaa aacaattaac tacttttaac
     2881 catacattcc atttaaaaga agaagttctc tcgcttactc gtgaagacga agttatcgag
     2941 attactacaa ataaaggcat ccattattcc aaagcagtca ttttagcttt aggaaatggt
     3001 tcattccagc caagaaaatt gaatttagat aatgctgaat catttgaaaa ccacggattg
     3061 gattatttcg tgaatgattt gatgaagtat gctggaaaaa aagtcgctat tgctggaggt
     3121 ggtgattctg ctatcgattg gacgttgatg ttagagccta ttgcaagcga agtttacttg
     3181 attcatcgtc gcccggaatt tcgtgcgcac gaacatagtg tatcacgctt gaaatcttca
     3241 tctgtcaact tgcttactcc ctatctcatc gatggattat caggcaataa tggggaactg
     3301 acagatatcc ggttaaaaaa agtgaaaagt gacgagacga ttgatttgat gattgattct
     3361 ctgatcgtca attatggatt ctcttctaat ttagaacatc tttcttcttg gggacttgat
     3421 agtaccagaa atgccattac tgtaaaatca gatatgtcta cttctattcc aggcgtttat
     3481 gcagctggtg acatctgtac ctatgaagga aaagtgaaac tgattgctac tgggctcggt
     3541 gaagcaccta ctgcagtgaa caacgcttta cactatatta atccaaagga acgaactcag
     3601 cctggtcaca gcacaagctt atacgataaa aatcttcgtc ctagttgatt ttttatatct
     3661 ctttgcattt caagttaaac ccgaaagcag tttcttgcat ttttttcatg ttccgttaac
     3721 atgaaataga gaaagaaaaa ataaaggggg agcaataata tggtcgtcaa aactgagact
     3781 ttagaagaaa aggcaagaga actattgaag gaacgtggcg taactgtaga agatatcgca
     3841 caacttgtca tgtttttaca aaaagactat atttctgatt tgactttggc ggattgtact
     3901 gaaagtgtca acagcgtgct aacaaagcgt gaagtacata acgccattat cactggaatc
     3961 caattagata tattagcaga aaagaaaaaa ttgatgagtc cgttgcaaca tattatcgaa
     4021 gatgacgaag gcctttacgg tatcgatgag atcctagcct tgtcaatcgt gaatgtttac
     4081 ggtacgatcg gctttacgaa ttatggttat atcgacaagg tgaaacctgg tattttaaaa
     4141 gaactaaaca gccacgatgg cgttcatgtc cataccttct tagatgatat cgtcggagcg
     4201 atcgccgctg ctgctgccag tcgattagca cacaaagaac cagaaaaaag aagtcgcctt
     4261 actcaataat tcaacaaaaa aagaaagaaa acgtctattt agacgagttc tttctttttt
     4321 gtttgtttca agctattttt tcctaagaaa agaaaaacag caaaaaataa ttctaataat
     4381 acaaaaaata agataaagct atgcatgaaa aattcttctg gaagtacatt gatgattgga
     4441 tcagtaacgg gatcgaataa ccagtcatcg ttgttgaaaa acgtttcatg aaaaaggata
     4501 aaaaagcgat caaatcctat catcatgaaa aaaccgaaaa caactggcag taacatgccg
     4561 atttgaaacg gacgaatcaa ccgccaaagc cgatcattct tttgcaaata tttgataaac
     4621 aaaatactcg ggatcaaagt gatgaaaaac acagcataat ccactaaaaa caaaattttc
     4681 acttcataaa agtgatgggc tccgctagca gaaactggga aatctggcaa gctgaggatg
     4741 gttttgaagg gattattgag ataattcatt aggtgatcaa aatttttcag taacgtctct
     4801 tgatctaaag aagtataatc caaaatttgc aaatgtccga tatcaaatac gtatagtggt
     4861 cgaaagttga tcgtcacacc aactgccaga ctgatcagtg tcagaaatag gctgaacaat
     4921 ccagcatatt ccatccactg ccatctcgaa tttttcatat tagaagctcc attcatccag
     4981 tgattgtttc acataggtag ctgggatagg caacccaggt acatcttctt cttttgtaaa
     5041 acctgataaa acgagcaaag tatctatatt attgcgtatc ccagcttgga tatctgtttc
     5101 atagttatct ccaaccatga ttacttcttc tttttgcagc cctaatactt caacggcttt
     5161 atccataatg atggcttctg gttttccgat gtagatcggc ggcgtttggg ttgctgtctc
     5221 tacaaaagag atgactgacc ctgctccagg aagaaggccg cgttcagtag gtatattctt
     5281 atctgggttc gttccaatga aagtagcacc tttttgaata gcaagtgttg ctttaactac
     5341 tttttcatag gtcagataat tatctaagcc taccactaca tagtcaggag cctcttcttc
     5401 ccatgtaaat ccggcagcta aaatcaagtc aatcaatcct gcttctccga taacatagac
     5461 ttttttccct tttccatctg ctttcataaa atcgattgtt gccaaagtag ctgtgtagac
     5521 tgtctctgga gctacatgaa tgtcaaactc atctgctaga cgattagcta ctgtttctgg
     5581 tgacttagtc gtgttattgg taacaaacaa gaaaggtaat tttctttttt gcaattcttc
     5641 cacaaatcgt ttaccggccg gtatgggttg tgtcccacga taaatcgttc cgtctaaatc
     5701 aattaaatat cctttatagt ccattcatcc actactcttc cctttttctg atcgtaaatg
     5761 ctgtttttgt tttttctact tgtttaggtt gttcttgttt ttttactttt tcagaggttt
     5821 ttgctgactc tgtttttcga tttttaataa ccggtttttt gcgattagat gcgactggtc
     5881 cttttttctc atcgatatac gcacgattat ggtttttttt cttttttcgg cgctgagttg
     5941 tcttttcttt tttaccgccg attctctcga tgacaaaata cgcacaacca aaattgcaat
     6001 attcgtaaag atagtcttcc aaggaatcga ttcgctgatc tggatgcgcc cgtcgattat
     6061 ccgcttggaa aaatcctttt aggcgtagtt gttcataacc ccaatctcct acgatataat
     6121 catatcgggc tagtacatca ctgtaacgtt ctccaagctt ttctgcatta aatccttcac
     6181 gataatcgga cacaagccga taatgtcttt cacctatcaa aaaattactg tcatctaaca
     6241 gtgtcactag ctcgcctttg actttttctg ctttaggctc ctcaacgatt tcttccaaag
     6301 cagctgtcag ttcttcggtg actgttgttt cctgttcagc tgatgatttt ttcttagcaa
     6361 tttctgtttt atgctcaacg ttctgctgtt cattttgttt tgcagacatg cgttcaccac
     6421 ctatcttttt tattatacct tgtttttatt tgattgggta gtgagctttc aagtaatctc
     6481 ctaccacact tcggataaat tcagggaaca aaaattcatt ttttccagca atctcaatcg
     6541 ttggaaaaaa tggaacaaac atcaaatgat ctacggtagt aaaagtataa tttttattca
     6601 gatcgattgg cttgttcttc caaaagactt gatggttaat agaatcatat gtgattccat
     6661 tatagaccat ctgcccaaaa atcttaccgc gaaatcccat tcccttcata ggatagttac
     6721 gtaaaaagtt acgatttttt tctatttcta aaactaagcg gatcagatca tttccaccca
     6781 atgtgacatt taacaagtgc atcggatgcg gcaacgcagt gtgaagctga tcctgattga
     6841 cccgcccttt tggaatttca gttaaaaaca gaccactgtt taatattgct gcttccgtct
     6901 ttcctcgttc tttcaccgcc tcaaaggcta cttggataaa cgaatgctca ttgaagatat
     6961 ctagactcag agaaaatggc aaatctgcta cttctttttc ttttaataat tcatgcccac
     7021 gttctagata actatttatt tcttgttcat cttctgtaaa agctgtcatt gtttcggtag
     7081 ggatggcttt ggctgaatgc ttaaggattt ttttctcttc atcgatggtt aaatggactt
     7141 caccgacata ctgcccgtac tttccagcgg ctgctatttg gacatcattg attacgtgac
     7201 cttctctgaa taaatgatgg gtgtgcgatc ctaaaataac atcaattgct ggtatttctt
     7261 ttgcaatttg gaaatcatcc tgaatcccga gatgactcat caaaactaaa acatccactt
     7321 gtgaacgtaa atcttcgact aattctggca aaatatcata aggattccgt atgtcccagc
     7381 cattcggatt ataagtaagc ggaaatggag cagtaaatgc aattagtccg attttcgtct
     7441 ggtgtttcgt tgtaatgatt ttatatggtt tcgtccatgc cggtggctgg agcgtatttt
     7501 tatcgaataa gttatccagg atgatatcaa actcagcatg atcgtataaa tgattcaact
     7561 cttctttgga atttcctacg ccttcattgt ttcctatcgt cgctgcatca taaccaactt
     7621 cattcatcag caagatattt tcctgtccat tcgttgcttc tgacaatgga tgccaacgat
     7681 ccacaaaatc acctagatct actgcaatcg ttgtcgtttt ttctttcttt tcgttttctc
     7741 gtttacgttg atccaaaaac cttctgatcc ttggccaatt ttctaaatga gaatgcagat
     7801 cattcgtatg taaaatcact attttttcca tgtaccctct cgcctctctt tatctttatc
     7861 ataccatagg cagctttttc ttccattgaa acaagctcta tttcagagtt tttcctgcct
     7921 tgaagcaaat tgtataagta caatattcga taaatcagtc tggctaaaaa atcaggaaca
     7981 ttcattttta cagtactata aaaataaaaa aaacagaccc gaaagtctgt tgtcgtttca
     8041 ttataatcag acacatggcg gaacctgctt agtgctgctt ccttccagac ctgacacgct
     8101 tcacaagccc accattgtcc tagcctttcg gcaaagaata gtatacctta tttttcattc
     8161 gttgaccagt ctttttttaa tttctttttt cgctggcgga gctttcggaa aaagtcagtc
     8221 aaaatcatcc cacattcgtc ttctaaaatg ccaccttcta catatgcttg gtggttaaac
     8281 cgctcgtcct ccaaaagatt catcaaggta ccggctgttc cgccttttgg atcatacgcg
     8341 ccgaaataaa cttctgctac acgagacaaa atcatcgcgc cgctgcacat aggacaaggc
     8401 tctaaggtaa caaaaagtgc agcatcttcc aagcgccagc ttcccacttt ttcacaggct
     8461 tccttgattg caagcatttc tgcatgtgta gtagcatctt gcgtggtttc tctaaggtta
     8521 tatcctcttc caatcacttt tccatccaat acaaccactg ctccaatcgg aacttcatga
     8581 agcatttctg ctttctttgc ttctgccaat gcaaggcgca tccatttttc tttttctact
     8641 gcatcaagat ttgtatttac caattaccct tccctgcctt tgttttttct tgttttactt
     8701 tagcatatga taaaaaggct gtaactaact tttttttcta tggtaaaata aatacaagag
     8761 gtgaacaaga tgaaaatgga taaattactt gctctttacc cacaagcaca gaaaaaaaac
     8821 tctccagcat cagaagcgga tatcctttct tttgcaattg atgatgcttt tatctggatc
     8881 aaacaagatt cattatctca acaagaaacc agccttttga aagcgttatt ccctgtaatc
     8941 aacgatcaaa aaaaacaccc atggtatcgc tatcttttta aagatgcttc ttgtgttcaa
     9001 gaaaaaagct ttcgtttgat ccagcttcac gtcgaatcaa aaggagaatt cttaaaaaaa
     9061 gaatggcaag atactatttt ggaattattt cgctattctg ctgatttctt tttttataca
     9121 gacacagatg cgctcttagt cgaacaaata aaccgtgatt atcttgatgc tactgaaatc
     9181 aacggtattt ttctatcttt agatgctgat tttgatacga caacgtctgt attcgttggt
     9241 tctttccatt cacctggcac cgacctcgtc cctttgtttg ctgaagaaag aaaaattttt
     9301 ttaacagagc aaaacaatct ttatactcac agccgtactt tttctatgca agatgtggct
     9361 ttacattact atacaaaaga tactgtaaaa gatagtaagt tgatcaaaag ttacatagaa
     9421 ctgatcaaac aaaatgaaat ggaagtgatc attcaagaat tatggaatga attaggcaat
     9481 gtcagctctg ctgcaaaaac actgtttctt catcgtaata ctctaaaata tcgaatcgaa
     9541 aaatttcaag aacagtctgg tttcaatcta aaaaaagcca acgatttact gttttgctat
     9601 cttttattgc ttcatgaaca taattaaaga tagattaaag gggttgtgac aataattttg
     9661 tcacaacccc ttatttcgaa tgaatagtgt tcaaaagcta gtttctctta catcattttt
     9721 tcaacaaaat gcattagccg gtcttttaca tcttcggttg aatctttgat tgaatcacca
     9781 taaacagaga ttttcttttt tcctgatttg atttttgcta acattcccat caaagaatcc
     9841 aagtcactat ctgaaagacc atctacgatt tctagtaatt tttcatttcc atcaaatttg
     9901 tcatagacga atttttttac tttcgtccga gtagtcatat gatggatctt ttccattact
     9961 tttcccgata caacgactgc tgtcgctaca ctggcagcgg ctgctacgct taatccgatt
    10021 gttacttttg ttgatgtttt cataagtatc agctcctttt tatctatctc catcatagca
    10081 tagacaaaaa aataaacgaa ctaatccact cagtgaaatc tcaatccttt agggaaaaag
    10141 ttctttactg ttccttttac ttgtttccca aatccgacag gtatcttatc taatactagc
    10201 aaaacccatc cttgattccc atcacgttga aatgtttctc ctgctgtgta agattgccat
    10261 tccttttgag tgatcggcaa gcaaggtata ttctctatct tttttgtagc aagcgctaat
    10321 gcatagctag gttcaaatcg attctttttg aaatctccta aatgcaaacc tgtacgtacg
    10381 acttttaaac catcgagaga aggggctaac tcaggaactt cccaaagatg gtcgttgaac
    10441 actaaaagtc ttccagtagc ttcataatga aaatcatttg aaaactccgt ccagagtttt
    10501 tcctgctctt ttgtcatttg cactttactt ttttttcgtg tcttcttttc tttatgcatc
    10561 tgattttgtc catgaaaagt cagctttgca acaaaatgtc cttcaccctg atctttatga
    10621 ggccatatac gtattgtttt ttccaaacca gctacgcttc cccattcaga acgtcctgaa
    10681 ctaacggatt gagtcaaagg aatctcctca atcgttactg gataattttc tacaagccag
    10741 ctaatgatct cttcattttc ttctggtgca aacgtacaag tcgaataaat cagttgtcct
    10801 ttatttttta gcatttttat tgctgaagac aaaatctctt gctgacgttt ttgacagtat
    10861 agcggagatt cttctgtcca ctcttttatt gcatttggat cttttcggaa cattccttct
    10921 cctgaacaag gagcatcaac cacgatccga tcaaaaaagc ccgaaaaatg agggacaagt
    10981 tctgcgggtg catgatttgt cactattgcg ttcgaaactc cccatcgttc tatattttct
    11041 gataaaatct tcgcacgttt cggaaagatc tcattcgtca ctaataaccc tttccccttc
    11101 atttgagcag ctaattgggt tgatttacca ccgggagccg cacacaaatc caaaactttt
    11161 tcaccgggtt ttgctgcagc agctgttcca acgatcattg cacttggttc ttggctatat
    11221 tcatacccag cttgatggag gaaagacttt ccattgacag tgccaagaaa tccctcattg
    11281 ctgtagggag cgggttgtaa ttcttcgcta tggtaagttt ggaccatgtc caatcctgcc
    11341 ggttttagtg gattccaccg aaatcctttt ttcacgcttc cttgttcaag cgcagaaaaa
    11401 aaatcggatg cttcttcgcc taaaagaaga cgatattttt tgataaattg ctgtggtaac
    11461 gttgtcgctt cctccttcat tccaatgttt cgttagacat tttacaatct tttttacgaa
    11521 aaataaaggt ggctgatcta ctttttcatg ttgctgccta aggtgactgt tcctttaaga
    11581 tattttccaa gtattcatcc gtcacctcgt ttttatccca aacagcgttc atcatttcaa
    11641 gaaaatcttg agaagaatca tctgagcgca aacttcccaa tgcattaagc cataccaatt
    11701 caagcgactt ttcttctttg atcttcgtgt gacctttgac atatgagaaa aatggccgct
    11761 taaatagcga atccatgtct aaatcagatt ttgcaatgat ttcacgaata ctttcatgga
    11821 tagaaaaaaa atctttcaat aagtagacaa tgcttaaaac atcatcggaa ctgcattcag
    11881 ctaagtagtt ttcaaaataa acagtgattt cttctaatat cttctctttt aacccgctga
    11941 ttgatttgaa atgtgtataa atcgggtatg tagaacaacc gcagaactct gctaactctc
    12001 ttgcagtaat tgacttcatt ccatattctt ccacgtatac catacttttg aacagcaaat
    12061 attctttact ataatttgtt actctcccca tcattcttac c
//
LOCUS       DACDMM010000076        12057 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDMM010000076 DACDMM010000000
VERSION     DACDMM010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.077"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(63..803)
                     /gene="truA"
                     /locus_tag="IWD16_001684"
     CDS             complement(63..803)
                     /gene="truA"
                     /locus_tag="IWD16_001684"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9088719.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(994..1629)
                     /locus_tag="IWD16_001685"
     CDS             complement(994..1629)
                     /locus_tag="IWD16_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9088720.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(1776..3017)
                     /gene="sstT"
                     /locus_tag="IWD16_001686"
     CDS             complement(1776..3017)
                     /gene="sstT"
                     /locus_tag="IWD16_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP9088721.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3215..4213
                     /locus_tag="IWD16_001687"
     CDS             3215..4213
                     /locus_tag="IWD16_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088722.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            4226..6244
                     /locus_tag="IWD16_001688"
     CDS             4226..6244
                     /locus_tag="IWD16_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP9088723.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(6304..7020)
                     /locus_tag="IWD16_001689"
     CDS             complement(6304..7020)
                     /locus_tag="IWD16_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9088724.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(7247..7789)
                     /locus_tag="IWD16_001690"
     CDS             complement(7247..7789)
                     /locus_tag="IWD16_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9088725.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(7794..8021)
                     /locus_tag="IWD16_001691"
     CDS             complement(7794..8021)
                     /locus_tag="IWD16_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP9088726.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(8100..9980)
                     /locus_tag="IWD16_001692"
     CDS             complement(8100..9980)
                     /locus_tag="IWD16_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9088727.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(10169..10783)
                     /locus_tag="IWD16_001693"
     CDS             complement(10169..10783)
                     /locus_tag="IWD16_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9088728.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            10925..11485
                     /locus_tag="IWD16_001694"
     CDS             10925..11485
                     /locus_tag="IWD16_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9088729.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(11790..>12057)
                     /locus_tag="IWD16_001695"
     CDS             complement(11790..>12057)
                     /locus_tag="IWD16_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP9088730.1"
                     /translation="FTQASDNLIHAFSNEWFVSEKELHASNLQYMPGEDPIPNMKAII
                     NSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT         3756 a         2404 c         1941 g         3956 t
ORIGIN      
        1 acattattta acatcgatat ttatactgtg aaacatgcgt ttcatccatt atcaaagtat
       61 ttttatcgat aataaacttc atccaaaaac agaccttgtg cagggacggt ttctcctgca
      121 tgttgccgca ctttgctttc aaatatttca tcgattgttt ctatcggcat ggcacctgtg
      181 ccgatttcta gtaatgtccc catgataatc cgcaccattt tatataagaa tccgtctccg
      241 acaaaagtaa aatgaagcat ttctcccttt cgttcgatag ataattcatc aatcgtacgc
      301 actgtggatt tttttgattt tttcaatgca gaaaaaccaa tgaaatcatg tgtacccact
      361 aatttttcgc aagctgcatt cagcttgtcc atatccaact cttgagggaa ataaaagctg
      421 tggtttcgct caaatgctga aggaatcacg ttgttccaaa cataatagct gtattttttg
      481 cccaccgcat tatatcttga atggaagcgc tcaggcattt cttcgattct tttgatgaca
      541 atatcccgcg gaaggtaacg attcataaag tcgatcattt ctgccagtcc aatttctgtg
      601 tttgttttaa aatttgctac ttgtcctcgc gcatgggtgc cagcatctgt tcttcctgat
      661 ccaataattt ctatctcttc atttgtcatc tgatgcaata ctttttcgat ttttccttga
      721 atggttttat cgtcatctcc aagtctttgc catcctgaat atctttttcc gtcatattcg
      781 attgtcaact tgatgtttct catattttag tggctcctct gtcttccatt tggtcttctc
      841 tctacctttt taagattgta gcacaaaata tagctccttg cttctctaag acagaagcta
      901 tacaagaaaa ctgtggaaat atatatttca gaaaaacaaa aaacagccac cggatgaagt
      961 atccgctgac tgttcaattt gacggctcaa gttttactgt ttgagatgca agatactcaa
     1021 cctggcaatg ggcacataat taaagaaaaa actgattctt tggcaaaaac ctattttatt
     1081 cagtaatggc caagaattta tagaaggaat tcttgccagt ccataaatcg ccgcaaaaag
     1141 caaactgatc acagacagtc caaggtataa gtggcttacg tccatttgcc cattttttcg
     1201 atagatcaag aaaagaagaa ctggaaacaa taaaaaacca ctgtaaccta aacctgatcc
     1261 gatattatgg acccatgtcg agacattcca cgaagattca tgggtattga tactgaacaa
     1321 acctgtaaag atacaatcac ctataccata gaacgcgatg gctgctgtaa ctagggtagc
     1381 taacacacga gatgtactaa caaatgcagc atacaatgca ggcaatgaca ggatgaaaaa
     1441 gatccctgaa accacagacc aaataagaaa cgcttttctt acaggactgg aaacctctcc
     1501 aaatacactg atcacgctct tcatttgatc taattcagga taatacaatc ctaatacgta
     1561 cggagtcaaa aaatcactca gcacgcctaa taataaaaag aaaaaaccaa atttacgtaa
     1621 aaaatccatg atacgcctcc aataacaatt acctatgctt ttaattgtta ttataaacga
     1681 ctttatcttg atttaacaga aacctctatt tacacgaaca tctatataac tcattaaaaa
     1741 ctcgaatcat tttaaaaata ctaaatatct aacgttcaac gttcacttaa tccatctaaa
     1801 caatctttca atttgatttt ttccccactt cttttacgcg cgcctagctc tcctattgca
     1861 gtaaacaaca gatcacttga cgaatttaag gcagtttcca ttgaatcctg gataacaccg
     1921 ataataaatc caataccgac gatttgcatc gcgacatcat tagagatacc aaacaaacta
     1981 catgctaatg gaatcaataa cagtgaaccg cccgcaacac ctgaagctcc acaagcagct
     2041 aaagcagaca aaatacataa aaggactttc aaaacaaaag gtgcttcaat ccctaaagaa
     2101 tgtacagcag tcaacgtcat caaggaaatg gtaatcgcgg caccgcccat attgatcgta
     2161 gcacctaacg ggatcgatat ggaataagac tcttttgtca atttcaatct ttcagccaac
     2221 atcatgttta tcgggatatt tgcggcagaa cttcgggtaa agaaagcggg aagtgcgctt
     2281 tctttcaaac aaaagaaaat caatggatag ggattttctt taataaacaa aaacgtgagg
     2341 aatgggtaca caactaatgc gacaaacagc atcgtaccga tcaaaacaag taaaagctgc
     2401 agatactctg ccaatccagc gattccagtc gtagcaattg attgataaac caatcctaaa
     2461 atccctatcg gagcaaatgc aataatcact tgtacgactt gtgacaacgc tatagatatc
     2521 tgatccacca actcttttgt cgattctgaa cgtgctcgaa aagctagtcc caacaataca
     2581 gcccagaata agacgccaag gtaattcgca tcaataagag attgaacagg attttgtgtg
     2641 acattcgtca acatagtaga taaaacactt tccaaacttt gcggtgcttt ttcagatact
     2701 gtttcttgta aagtaatagg aacagtaaaa attgaactta caataactgc tacaagtgca
     2761 gaaagcaatg ttcccactaa atataagatc aaaataggct tcacgaatgt cttattccca
     2821 attttatgct tggcaattga cgccatcgtc aagaaaaaaa ccagaatagg tgcgattgcc
     2881 ttcaagcaac tgacaaacaa cgttcccaat attgaaataa atgtccatgt ggggaaaaag
     2941 ataccaaaca atattccgac taaaatacca acaacaatac gttgaactaa cgacatatcc
     3001 aaaaacttct ctaacaaatg accacccttt cacaatttta acaatcattg tatcatttta
     3061 tgcaaagaaa ctaaaggtat aattttctaa aaattctaac aatacaaaca accatttctt
     3121 gctaattttg tactttttct gtctacacgt tgttttttga gatattctaa gttaggatgt
     3181 tattaatggg caacttatca ggaaacgttt tcttatgttt ttgtccaatt tttttgtgag
     3241 gtgtttattc ttgaaaaaaa taaagaagga gagttatggc ttcctgacaa tctttgtcgc
     3301 ttgtataatg atggttttac agcgtatatt gatttcagca gtcactattc ctacatatgc
     3361 aacaaaccct ggtcgattct acatctatac atttttacaa acttccgttg ttattggtgc
     3421 aaatttgatc ccactttaca tgggttacca atatcaaaaa atcgcccgtt taaaagtttt
     3481 acattattta agtcgttttg cctttatatt cctaatagcc acactagttt gcaatatctt
     3541 tttttacgta caagctggca gcttgaatat tcgtgattat tggcttatcc taacacctat
     3601 cagtcagaat tactttactt atgctgtttc ttgtactttg cttttttgta gtattcctta
     3661 tacagttgga tttttaaata aattatcgaa aacaacaatt aaatatctac tattattttt
     3721 aacaattgga ttagttggct gctctactct atttaataaa gacccgtttg cattacagaa
     3781 tggacagagt attttatgga ttttttattt atttttaatg ggttatggtt tgaaagaatg
     3841 gaactggaaa gaaaaggtcc gctataagct tcctcaagta tttatcagct tgattgtttt
     3901 attcggtttg attattttga tgacacaaat ttcactcatc atcagaggaa atacagatac
     3961 agcgctgagg ttttctaaac catattctct cttcgcaatg tattatactt tctcttcgtt
     4021 tttattttta gaaatgctat ctgataaatt aggtataaaa ggaagaggaa attttcttgc
     4081 tgtatttttg attgtcactc aagttatatg taatttccct ttagtttctt atcgcgtatc
     4141 tacatttatg aaacgtgaat ttccaaactc agggaaggca tggctgataa atatcgggca
     4201 gttttttatt tgatatttat tagcagtgtc cctcctagtt atctttttat tttatataca
     4261 aaaaatccgt ttcattcatc gtttcatcga atacttcgct tttgattcga ttgaacaatt
     4321 aactcagcgt tggaaaaaaa gaattcattg gctttttgtt cataaaagtt attttttagt
     4381 tgcagcattt ttttactgct ttaccttcgt gcagattttc gttttagaac caaaagaagg
     4441 ttggaaagaa actatccagg ttgccttaaa aatatttact cagcgacaag cacccatgat
     4501 cctgacaatc atcattatta tgggattttt cttcttacta ttgttgattt ccaatcgttt
     4561 ttggtacgcc ttgactgcta ccttgatcat taacttactg ctcactattt cgacagtaat
     4621 caaaatggaa atgcgggaag agcctgtctt tccttcagat ttaaaaatgc tgaccggatt
     4681 gtcagaacta ttatcgatgg ttagcccagt cttattaatc gtgggcggat tgatccttct
     4741 cctattattg attactagta ttattgtaca acgtagacta caaaaacagt attccttgaa
     4801 gattcattgg aaacgtcgat ttatcagtat cgctgtatta ttaggatgtt tttcgggtgt
     4861 ctttttcatt aatcacaaaa attcgccttc tttcttgctg tttaatttat ttaaagtaaa
     4921 caagactttc tttaaccagc aaggcgccgt aaaagataac ggaccagtca ttcaattcct
     4981 aaacaatatc gacattaaaa tcatggaaga acctgctggc tattcgaaag aaaaaattaa
     5041 tggaataatg aaaaagtaca acacaaaggc agatgaaatc aaccaaacaa gaagtgactg
     5101 gttaaacaat cagacgatcg ttctcaattt aagtgaaagc ttcagtgatc ctgcacgtgt
     5161 ccctaatctg acagtaccta cagaccctat tccttacatc aaaagcatta taacagaagg
     5221 aacaggagga ttgatgttat ccgtcggtta tggtggcgga acagctaaca tggaatggga
     5281 aggactgaca ggcttgagta tcagtaacct gtctgcttcg ttagttacac cttataccca
     5341 gctagtggag cgtcagaaaa taagtccaaa tattaccgat ctattcgatg aaaaaatagc
     5401 catccatcct tttacagctg ctctttatcg cagaaaacaa gtgtttgata aatttggttt
     5461 tgacaggttc tattatttag acagtccgga taaattaact tatacggata aaatagaaca
     5521 aagcccacgc atatcggatg attcagcata caaagaaacc ttgaaagctt tacaaagcaa
     5581 tacggaaacg tcacaattca tccaattatc cactatgcaa aaccatatgc cttatgatga
     5641 ttactatagt gaattgaact acactgctga aggaactgct gtcattccta atagaagaaa
     5701 agaattacaa acttatatgc aaggccttca ctataccgat acagcagtaa aaaaattcat
     5761 ccaagaaatc gacaaaatcg ataaaccgat tacctttgtt ttttatggtg accatttacc
     5821 cgcaatctat agtgggaatg attcgaaaaa atatggatta gaacagcacc agacagatta
     5881 tttcatttat aacaacaagt atagtcgtga acaagcagct acgttaaata aagaagttgt
     5941 tgctcctaat aactttggtg ctatggctct tgagcatgcg aatatcaaag taactcctta
     6001 ctatgcgttg ttgacagaag tatctaatca tctacctgca ttaacaattg atcctacaga
     6061 aagcttgtct aatcgtttta atggaaaaca ggtctttgtt acagaggata gtcaaattat
     6121 ccaagaaaaa aatttaactg agaaacaaaa agaaattcta gaagattatc gtctgataca
     6181 atatgatctt gttgcaggta accagtacag tgcaacatgg gcgacacaaa aaataaaaga
     6241 ctaacaaata aactgagcaa ggggttgtga taaaagacat gtcacaaccc cttgtttatc
     6301 tcattaattt gcttgtccat aaacgatttg attaaagcca tttttcaatt cattattcgc
     6361 tgtctcgttg aagtaaagat aattatcggg attcccctga ttttgttgtg tcaccaatac
     6421 ttttggctga ttttgctgaa acccgaatag atagacgtgc tttttcagat atggttctgt
     6481 ctctgcatcg gaatagacgg ctgtgatttg atagtctgct tgacctgtcc cactttctga
     6541 ccattctgca gaaacaggca ctccaccaat agccatcttc cactccccgt tcaaaatagc
     6601 ttgagggact ttaagaccat ataagtccac actcatttga ttcgtataac ttttgtattg
     6661 ttgtcccatt gtttggcccc atttggtcat aaatgcctct aattgtgcgg atttgtctgc
     6721 attccacagt gtttgttcaa cttgttcgct gctttctgaa gaggaagatg aagcctctac
     6781 agaagaactt gaagtagatg tttgagttgt ttcagtcgtt ttggaagact ctgacgaagt
     6841 agaggtatct gctgtttctg tacttgctgc agttgacttg ctctctgtgc tagttgttgt
     6901 agtagttgca actgttgctt tcgctgtttc ttttgttgat tcttttttcg attcattccc
     6961 gttggtacaa ccagctaaca aaaaagctag accaattgtt actaaaatcc tttttttcaa
     7021 ttaaaaaact cctttcgatt actcccactt ctttattcta tcaaataagc aatgtgattt
     7081 tattgatttt tcgtattttc tcatcaatct ttcaaatctt ctaaaaactg agaagcaccc
     7141 gctgatgttg tctgtaatac atcagcgggt gcttctttta ttttaatctt cgtgattttc
     7201 caatttacct cacgctttat atttgtattt gaagaaacat agacttttag tcatcatcgt
     7261 cttcctcatc caaatcttgt cggacagttt ttccttggag tctttgtact tggtagatga
     7321 tttctttatt gaaacttaaa agttcaacaa gcaattgttg caagccatat ttttcttctt
     7381 tttcagctaa tttgattgcc cgtgtgataa agacattttg gaaatcaaaa tcttttgcaa
     7441 agttttccaa cattgttgag gctggtaaat atttgttttg accatgttcg taaatttttc
     7501 cataacgcat gacttcttct aaagtagacg gaacgatttc attttcaatc actaactcat
     7561 gagagatttt attgaaccat ctcgcttctt gatcagcata ttctgaaaaa agggtactta
     7621 attcttgcga agatgtaccc aaaacataca attgtgcttg gatcaatttc atttcttcta
     7681 atttaaaatt tgctaataca tgccccacca tggcgccagc agttggttta tgatggtcga
     7741 tatctgcttg ttttgtttct tgaacaaatt tttcttccac tatattcatt gatttacgcc
     7801 tcttttactt ttgctgattg gacttcataa cctaattttt ccacaactgc agctaattgt
     7861 tcagggtgga cttcatctgg atcaaagttt gctttgattt tgctcgcatt aaacaaaact
     7921 ttcgtattgc ttaccccagg ctgttgagaa accgcatgtt cgattttttg catacagctt
     7981 ggacaagcta gtgtatctaa ttgcataata atttttttca tcgtagttct tccttccttt
     8041 ttcttgatag tcttattcta gattacagct tccataaaaa aattgattct tatcaagttt
     8101 tacgattttt cttctttgct ttcgtgtcca aaatacaata aacgcatcgc gttaaagata
     8161 acgatcaaga tactagcttc gtgaacgaac atcccactag ccatgtaaat aaacccggca
     8221 aataagccgt tgagtaaaaa taagacgaca ccaatggcaa tcactacatt ttcacgggta
     8281 tttaaaacag ttttcttagc caagcggtag gcatgaacca acgattcaaa attagattgc
     8341 atcaagacaa cgtcactcgt ttcgatagca acatccgttc ctgatcccat agcaatcccg
     8401 atatcagccg cagccaaaga gggagaatca ttgattccat ctccaataaa agcaacgcgc
     8461 aatccttgtt cttggtattt tttcacaaat tgaacctttt cgtctggtaa taattcagca
     8521 tggacttcat caatgcctaa catatcagct acataatcag cagtcatctg attatcacct
     8581 gtcagcatca ctaactgttt tgctcctgct tgtttcaact tagctaattg ttcagcgact
     8641 tctggtcgaa tcacatctga cacacctatg atttgagtga tttttttgtc gattgctaca
     8701 ataatgaccg agctgcctgt ttgttgtaat tgatgtaaat cttgtacttg ctttggtgtg
     8761 agagaaagtt tgtgagcggc aatcaatttg gtgttgcccg cacaaactgt atgaccatca
     8821 atctcagctg tgattccttg tcccttgatt gtctgattat ctttgactgc taattgttga
     8881 aaatcaatgt ctttctcctg agcataatgg atcacggcgc gccccagagg atgatcggat
     8941 aacgtttcta atcgagccac taaagctaag ctgtcccctt catcctctgt atagttcttg
     9001 atagtggata cagcagtagt tcctttggtc aatgtaccag ttttatcaaa gacaaaagta
     9061 tcgattttgg aaaaagtatc cattgcttca ccgcctttga ctaagatgcc ccgtttcgct
     9121 ccattaccga ttcctgctac attcgaaaca ggcgcaccaa tgactaaagc gccaggacaa
     9181 ccaagaacca aaacagtgat tgctaaacga aaatcacgac taatcagacc aacaaccaac
     9241 gcaataagta aaacggttgg agtataatac tgagaaaatc gatcaatgaa tttttccgca
     9301 tgggatttgg tatcttgtgc ttcttctact aattcgataa ttttggcaaa ggtcgtatca
     9361 tctccgacct tagttgcttc aatcgtcacg tagccatctg ctaaaatcgt tccagagaag
     9421 acttcatctc cactgatttt gacgacttgt cgtgattcac cagtcactgc ggcttcatcc
     9481 gcataccctt tcccgtcaat gactcgtccg tccacaggaa tagctccacc agtttttact
     9541 aagacgatat cgccttcttc cacgtcatcg atttccactt cttcagtcgt tccatcttct
     9601 tggacaacac tagcagtaga tggggccatc tcggtcaaag ccttgattga ctcccgcgtt
     9661 ttggtcaatg ttttttgttc cagatagcta ccaaacaaaa acaagaatgt aacgatagct
     9721 gactccttgt attctccgat cataaaggca ccaagcacag caatagtaac gagtaaatca
     9781 attgagataa ctttgacttt cactgcctga tacgcatgga tcataatagg tatgaccgca
     9841 ataatagaag ctatggctag agcaatgtta tagaacaaga caaaaccagt aaattttccg
     9901 ataaaaccaa aaataatcaa cacagctgat aaggcggtga tatgatttcg atgctcttgg
     9961 atccactttt gtaatttcat ctttctccct cttttccttt cattcttacg agactaagta
    10021 tagatactta tccatcataa aaaattgacc actatcaagt ttagtcaaaa aggcggtttt
    10081 cttttggtag aatacactac aaaaaatagt tagcaagaag actcactaaa agtgatcttg
    10141 ccaactattg tcgtttctat cgaaattttc attgaatgat tttcctgtac agcatacatc
    10201 ggagatctat ttctttatct aagattttta tcttgctaat tttttccttt agtctttcaa
    10261 atccacgtcg ttcatagaat tgatacgtac agccactatc tgtaaaaagg aatatttctt
    10321 ttcctttctc tcttttttca aattctgtta gcaacttggt accgatgcct tttgttttca
    10381 aatcaggatt tgcagctaaa aaaatgatct ctccatctgg cgaatgcttc tttaaatatg
    10441 ctgcaagcat ttgttcattt gctgtctcgt aagctccttt actttcttta gcaaatatat
    10501 tttgtaaaaa atcgaatatc ttaacatata tttgctgtcc aaaagaacga tgtttctttt
    10561 tttctccttt tatctccgcc agcaaaacac ctgccaactc gtcctctgca tacaaagcaa
    10621 gaatttgagt tgctcgattc aactccatat acaaaaaata tcttccgtaa agattcagca
    10681 gaagttcatt ttccatatat ctctcaaagt tcattccttc aacagcaaaa tagatagctt
    10741 ttttgtaatc tttcttttct aagggtttaa aagttagttt catcatttcg cctcctcaac
    10801 ttcaatgatt gtgttatact tacattataa tagacacagt gtctactatc tataatcaat
    10861 catctataag tttgtcgatt aagcgacagc tttgctttga tatgtcggat aaggagatag
    10921 gtaaatgaaa aagacagata gacgcgtcaa aaaaacagaa aaagctttgg ctgaaacact
    10981 atccacatta ttagtaaata aaaagattca agcaatcact atacgcgaac taacagaaac
    11041 ggcagatgta catcgttcga ctttttatac acattataag gatatctatg atctatatga
    11101 tcaattagaa agcaactttt ttcgggatct aaatgaaatt ctctcttatg atccagccca
    11161 tagctatgag gaactataca ctcgcttgat tgactatgtt tacgcaaata cttttttata
    11221 tcgcttattt atcctcaacc atggtgatat ttcgtttcag aagaaagtta aacaagttat
    11281 tgaagaaaac tatctgaaca tatggctata tgaagacaag aaaacgatta tcacagaaga
    11341 aatgagattc tttactacgt accacgtcca aggttgtcta tccgttctca atcggtgggt
    11401 agaaagcaat tacaagtact ccaaaaaaga attattagaa ctcttacaga aactgaatga
    11461 tcacatagag aaaatcatgc cttgaataaa aacatactaa gcttagttaa gaagaaccgt
    11521 aaccttctcg tataacaact gaatcttatt tagccaaatt gtttactgaa gattttttga
    11581 gtaaaatttc tataattcct acttattgaa aagtagtttg tagtgctgct tctccctctc
    11641 taagtgatat atcttgttgt accaatcaaa ttcacctcat cattaataaa ttacttttag
    11701 gctaatttat tacaacttat aggttgaagc tgtaaccaat ttatagtcat attatatttt
    11761 tatcatccag tttattgaat tcttctcgtt tacctcaatt cattttcaag gggaatcagc
    11821 tcatcatcta acattttacg ccatgctcgc ttcatttctt gtgggtattt aaatggtttc
    11881 gcttctggat gtttggcttt atatccttcg taatccttac tgttgataat tgctttcata
    11941 ttaggaattg gatcttcacc tggcatatat tgcagattag aagcatgtaa ttctttctca
    12001 gaaacaaacc actcattgga aaaggcatga atcaaattat cacttgcttg tgtaaaa
//
LOCUS       DACDMM010000077        11689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDMM010000077 DACDMM010000000
VERSION     DACDMM010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.078"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            12..2030
                     /locus_tag="IWD16_001696"
     CDS             12..2030
                     /locus_tag="IWD16_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9088731.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            2457..4871
                     /locus_tag="IWD16_001697"
     CDS             2457..4871
                     /locus_tag="IWD16_001697"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP9088732.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            5219..6772
                     /locus_tag="IWD16_001698"
     CDS             5219..6772
                     /locus_tag="IWD16_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9088733.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(7153..8937)
                     /locus_tag="IWD16_001699"
     CDS             complement(7153..8937)
                     /locus_tag="IWD16_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9088734.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(8952..10673)
                     /locus_tag="IWD16_001700"
     CDS             complement(8952..10673)
                     /locus_tag="IWD16_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9088735.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            10773..11534
                     /locus_tag="IWD16_001701"
     CDS             10773..11534
                     /locus_tag="IWD16_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9088736.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3810 a         2140 c         2378 g         3361 t
ORIGIN      
        1 gaggatagaa gatggaaaac attgcaagaa aagaacggag acgtttagaa cagaccaaac
       61 gttttcgaaa agcaaaacga ggggcagcaa tcgtgggaac tgcgatggta ggctgttctg
      121 tcgctgcccc attagtacag ccggttcctg ttcaagcgga tgaaacacct gttcagttcg
      181 gcgcaagaat caatacagcg gcatttatca cagaaattgc tacttatgct caaccaatcg
      241 ctcaagccaa cgatctatat gcatcagtga tgatcgcaca agcagttgta gaaagcggtt
      301 ggggcagtag tgcgttatct caagcacctt attataatct ttttgggata aaaggcagct
      361 atcaaggaca aaccgtctac atggatacgc ttgaattttt aaataatcaa tgggtaaata
      421 aaaaggagcc tttccgacaa tatccttctt ttgctgaatc tttcaatgac aatgcctatg
      481 tacttagaaa tacttcattt ggcaacggtt attactatgc cggtacttgg aagagcaaca
      541 cgaaatccta tacagatgcg acagcttgtt tgacagggcg ttatgcgact gatcctggtt
      601 acgcaggcaa attaaataat atcatcgcaa cttacggatt gacccaatat gatacaccag
      661 ctagcggaaa cgcaggcggt ggagcgacta caggtggcaa taatactgga agtgcaggta
      721 gtactggcgg ttcaagcaat tctggtaata caaactcagg ttcagcaact tcgaccactt
      781 atactgtcca atctggtgat tctgtttggg gaatctcaaa taaattcggt atcacaatgg
      841 atcaattgat ccaatggaac aacatccaaa acaatttcat ttatccagga caaaaactga
      901 caatcaaagg tagccaggaa aatggctctt cgacgaataa ttccggtaac aataccaatt
      961 cttcaggaaa tgctggtaca tcaaacggag gacaaacaac tggtgcgaaa tacactgttc
     1021 aatctggtga ttctgtttgg aagatctcaa atgatcacgg aattacgatg gctcaattga
     1081 ttgaatggaa caatatcaag aacaatttcg tttatccagg acaacagcta atcgtatcaa
     1141 atggcggttc ttctaatgct ggagcaaata ctggctcaaa tactgggaat acaagtggct
     1201 ctagttcgtc cagctcaagt tcttctaata cagcttcagg aacgaaatat acagttaaag
     1261 ccggagactc tgtttggagc gtctcaaata aatacggaat tacgatggct cagctaatcc
     1321 aatggaacaa tatccaaaat aactttatct acccaggaca acaactgact gtttcaaaca
     1381 gtggatcaac aaacagcacg tcaggtaata caggttcgtc aaccaataca aataacagca
     1441 gttcagttaa tactggcagc agttcgtctc aaggcactta taccgtgaaa gcaggtgaat
     1501 ctgtttggag cgtggcaaat aaaaacggca tcacgatgga tcagttaatc gaatggaata
     1561 atatcaaaaa caacttcatt tatccaggac agcaattgat cgttaaaggt ggaacttcgg
     1621 caaatacaaa tacaggttca actacttctg cagcgaaacc aaatacaccg aatacgtctg
     1681 ctacgacaag tacatcatcc actggtaata cgatgtatac cgtgaaagcg ggcgagtcag
     1741 tttggagcgt agcgaacaaa catcatatca cgatggatca gttaatcgaa tggaataata
     1801 tcaaaaataa tttcatctat ccaggacagc aagtgattgt gaagaaaggt tcatctcaat
     1861 caacaaacca acaatcagtt actggttcga aaacctatac tgtgaaatct ggagaatctg
     1921 tttggagtgt tgctgacagt catggaatta caatggctca actaatcgaa tggaacaata
     1981 ttaagaacaa ctttatttat ccaggtcaaa cgttgattgt agctaaataa aatagctgtt
     2041 cgaagaaata acgattgaat tccacaaaaa tatttgttac tataaggcta cttaaatatg
     2101 acaacacttg aaaaggaagt agtaaaaaga ctgaagtttc agagagcgca tggctggtga
     2161 gaatgtgcac ggatgctttt gaactcgcct ttgagttgcc cttgctgaat acccgagtaa
     2221 gcaagcggcg gtgacggccg ttacaacgtt cgaggccttt tcactaaggt aaaattaggt
     2281 ggtaccgcgt agattacgtc ctataaaaag agcaatcttt ttataggatt tttttatttc
     2341 ttttgtgaag tttgagaaaa caaagcaaaa ggaaataaaa ataaacagat tctgccggtt
     2401 ttttcgttat atttataata gaaacaagtg ttttcaatag aacaggagga cagcaaatga
     2461 gttacaatca taaagaaatt gagaaaaaat ggcaaaaata ctgggcaaaa acgaatactt
     2521 tcaacaccca tgatgatcct gaaaagccaa aattttacgc actggatatg tttccctatc
     2581 catccggtca agggctacat gtagggcatc ctgaaggtta tacggcaacg gatatccttt
     2641 cacgtgtgaa aagaagtcaa ggatacaacg tgctgcatcc aatggggtgg gatgcattcg
     2701 gtctgcctgc tgaacaatat gcgctagata caggaaacga tccagctgag tttacccaaa
     2761 aaaatatcga aacattccga cgccaaatca attctttagg atttagttat gactggaatc
     2821 gcgaagtgaa tacaactgat ccggagtatt ataaatggac acaatggatc tttacgaagc
     2881 tttatgaaaa aggtctagct tatgaagcag aagtagcggt aaactgggta cctgaattag
     2941 gaacagttat ttcaaatgaa gaagtgatcg atgggaaaag tgaacgtggt ggctatgatg
     3001 tagtcagaaa accaatgcgc caatggatgc tgaaaatcac tgcttatgct gatcgcttat
     3061 tggatgatct agaattagtt gattggccag aaagcattaa agaaatgcaa agaaattgga
     3121 tcggtcgttc agtaggagca aatattgaat ttaaagtggc tggtacggat aaatcctata
     3181 ccgtatttac gacacgacca gatactctat tcggtgcaac ttactcggtt cttgcacctg
     3241 aactagatct tgttagagaa attacgaccc ctgaacaaaa agctgctgta gaagcttata
     3301 tcgaagagac agctaaaaaa tcagatctaa agagaacgga tcttgcaaaa gaaaaaacag
     3361 gagtatttac tggtgcttat gcaatcaatc cagttaatgg caaggaaatc ccaatctgga
     3421 tcgccgatta tgtattatct tcatacggca caggagccat catggctgtt ccagcacatg
     3481 atgaacgtga ctttgaattc gctcaaactt tcggactaga aattcttcca gttatcgaag
     3541 gtggagatgt tgaaaaagct gcttacacag aagatggtac gcacatcaac tctgaatttc
     3601 tagacggcat gaacaaacaa gaagcaatcg ataaaatgaa tgaatggcta gaagaaaatg
     3661 gcgtagggaa aaaagaagtc agctatcgct tacgtgactg gctattctcc cgtcaacgtt
     3721 actggggtga accaatccca atcatccatt gggaggacgg aacagtcaca gctgtcccag
     3781 aagaagagct accattacgg ttgcctaaga caaaaaacat caagccaagc ggtactggag
     3841 aatctccatt agcaaatatc gaggagtggg tcaatgtcgt tgatcctgta acaggtaaaa
     3901 aaggccgtcg cgaaacgaat acaatgccac aatgggcagg aagctcatgg tattacctgc
     3961 gttatatcga ccctcataac aaaaaagaac tagcagatta tgaaaagttg aagcgttggc
     4021 taccagttga catctacatt ggtggagcag aacatgcggt acttcattta ctatatgcac
     4081 gattctggca taaattctta tacgatatcg gtgttgttcc tacaaaagaa ccattccaaa
     4141 aactatacaa tcaaggaatg atcttaggcg aaaacaacga aaaaatgtca aaatctcgtg
     4201 gtaatgtagt caatcctgat gatgtggtcg aaacttacgg tgcagataca ctgcgcatgt
     4261 acgaaatgtt catggggcca ttagatgctt cgattgcttg gagcgaaaat ggcttagaag
     4321 gaagccgtaa attcttggat cgtgtatggc gtttgatcgt tgatgagaac aataaaatgc
     4381 gtgaccgtat cactacattg aatgatggca agcttgataa agtgtatcat caaacagtga
     4441 aaaaagtgac agaagactat gaaaatcttc atttcaatac agcaatctcc caattgatgg
     4501 tctttatcaa tgaagcctat aaagtggacg ctctgcctta tgaatatatc gaaggatttg
     4561 ttcaactact cgcaccgatt gctcctcaca tgggtgaaga actatgggca atccttggaa
     4621 atgatggtgg tatttcttac gcaccatggc caacatacga tgaagcagca cttgttgaag
     4681 acgaagtaga agttgttttc caagtaaacg gtaaagttcg tgcgaaaagc aatgttcccc
     4741 gagacttagg aaaagatgag ctagaaaaag cagcattagc caatgaaata gtgcaagaat
     4801 atattgaagg caaaacagtc agaaaagtta ttgcggtacc aaacaagtta gtcaatatcg
     4861 ttgctaatta attggctctt tgtcaataag gactgatgag ttgtgcaaaa tcaaatctgg
     4921 gccagaatga acttcattct gtctcagatt ttttgttatt tcaaattgat taattgattg
     4981 atcaaaaagg ttttgatttt catgttctca aacacgttca atgtcttttt cttctgaatg
     5041 gtcttgtaag agatctctgt aatcatcaaa tttttgtcta ttaatcttag tattttttga
     5101 tgtataaact acctgaaatt gagagtacat accaaattca taaatttttt attagtggga
     5161 ctagtgattg tatggatttt gacgcataat gaatgaagta tgagaaaagg agagacaaat
     5221 ggatcaagaa ctatttatta tcgaagtatt atcagctaaa caaaaaatta tcgagaaagc
     5281 gaaaaaaaga gcgaaaagaa taatgttcaa gcttttaagc ttagtatttt tctctggttt
     5341 gctttttttc ggagctgtat cgttcctata tcgccatatg agtgcagtag tccgttcagc
     5401 tgaagggatg caaaaattaa ataatttgta tcacttttcg tggggagttg gcggaataac
     5461 aattgttgtg cttattgttt ttcttctact tatttggctc cgttaccatc aattgtcgcg
     5521 tcatttcgat caatggctta gtcaaagagc acaacagttt ttacataata aaccaacttc
     5581 tcaagatgat gactatcttt attttgagaa gacttcgacg agtcaagctt ttcggataca
     5641 aaaaagtgat tgccagttat tgctgagatc acttgatgat ttacctatct acttaggcga
     5701 aagcaaatca aaattcggaa tgaacacatt ccatattttt ttactagaaa acgaacccag
     5761 atgggaaaaa agccagtctt cgcctggtgg atacctgaca actaagatga gtacattagt
     5821 cactggtttt ttcctcattc ttttagctgg aacaatctat tatgggagat acactcagcc
     5881 aaaaatgtct tctcatataa atgctgcttc tcacatgaca atgaactcaa ctaacgatac
     5941 gcatatacaa aagacaaatg aagggttgtt agctgatcct aagcaaaagg taaatttgac
     6001 aacacaaaag ataaatgatc tcaaacttga tacagagacg cactctctct atatgacaac
     6061 agatagtggt gctaattggg tatttgtgcc gattgattcg caatggttgc gttttggcga
     6121 atacaccctg acaacggggc tgattcctga aggttattgg atggacaaca catatgatat
     6181 ttccgccgat ttctcatggt ttatctattc ttctggggat gatgtacgtc tgctgacttc
     6241 aagagataat ggtaaaacat ggcaaacgtc tattatcacc caatcagttc agccgatacg
     6301 ttatcgaaaa gcggtatttt atggcaacga tggtctattt atctgtacat cagccagtgg
     6361 gatgtctgca gaaactttgt acatttacac tactatggat ggcggaatca cttggataaa
     6421 aagtggaagt acaatgatca atcagccagt acaaaatgtc agcttcgtta caacttcttt
     6481 aggttttgtt tcgacacgtg aaagtttgtt ttatacaaac aacggaggac gctcattcaa
     6541 agaatcagtt ctgtcgattc cggaaaacta tcagacaggg gggatagatc tttttcaatc
     6601 accaaatgaa gttactcaaa taagcgcgaa tacgttagag acaaaatttt atttgttgaa
     6661 aaaaggacca atcgatgcag ggaaaatgtt tgcctgcctc ttccgttcaa ctgataacgg
     6721 ggaaacatgg caattcgttc agcaactatc gcaagtagaa caaggagatt aaagaagaga
     6781 gattgtgagc ctgacgttca gcaccgagtc acaagaaaca aattcaaaaa acagcttctc
     6841 atatttgttg aatagtgtaa ctcgtgattc ggagttgaca agagcaacaa gaagcgattt
     6901 tgaagtatag cttctcgtat tttacaagat cgtgtcttat tgccgatagg ttgtctcttg
     6961 aacaccgttt attcgtaaaa agggattgtg acaagtcttt tgtcactact taccgaacga
     7021 aactgaagga gctatccaaa aagtatcatt agtattattc tttgtgtttc tagtggcacc
     7081 actagtacat ttaaaaaagc tatcgatcat ggactaatca tacatgatcg atagcttttt
     7141 agataatgtt tgttacgcta tctgaaattg actattataa agactggcat aaagcgttgg
     7201 tgattggagt agttcagaat gtgttccttt ttcgacgata tcgccattct tcattacaag
     7261 gatcatgtct gcattttcaa tcgttgacaa acggtgtgca atcacaaaac ttgtcctatc
     7321 ttttgtcaca gcttccattg cttgctgaat caatgcttct gttcgtgtat ctacgctaga
     7381 agttgcttca tctaaaataa cgacaggagg attcgctaga atgactcgag caattgtcaa
     7441 taattgttgc tgtccttgag ataacgtgcc gttttcgcta gaaatgatcg tatcatatcc
     7501 gtctggcagt gttcgaataa agtgatcaca ttgtgcgatt tcagccgctt cgataatttg
     7561 ttctcttgtg gcatccattt tcccgtaggc gatattatca gctaccgttc cttcaaacag
     7621 ccaggtattt tgtaaaacca tcccaaaaga acgacgtagt tctccacgtg aaagcttggt
     7681 aatatcaaga ccgtcgaatt cgatctttcc tttattcagt tcataaaaac gcatcagcag
     7741 attgactaaa gtcgtttttc ctgcaccagt tggaccaacg atcgctaccg tttgttttgg
     7801 ctttactgaa aaatcgactt gattcatcaa tattttatct gatgtgtaac caaactgtac
     7861 atttttgaaa gctatttttc ctttcggttg ttctaaaagt cttgcatctg gaaggtctgg
     7921 aatttcatct gtttcatcta atatctcaaa tatcctatcg attgctgcca ttgcggattg
     7981 gatcgagttg atcacataag aagcggtaga aattggttca gaaatctgat tgatgtactg
     8041 aagataagct tgtaagaagc cgatcgtcac accacctgat agaacaagca tagcaccgaa
     8101 taccgcactt atgatgaagg cgagttgatt aatgaaacga atggccggat agatagcaca
     8161 gttcataaat tgagcacgtt taaaagcgtt gtattgcttt ttatttactt cgtgtatagc
     8221 agcttctgca tgagcttgct gattgaatgt tttgaccact agattaccag ctaagatttc
     8281 ttccattcga ttatttaaaa cacccagttc atactgattc tgttcagaaa tctgtttgtt
     8341 tttgttcgca atcttagttg taacaattga actgccaacg atcagcaata agacgagcaa
     8401 agtaagcttg ctttcgatat aaaacagcat cagaccagca gcagctattg ttacgacaga
     8461 agtaaaaaat tgattgatac ctgtcaataa cacttgagaa agttgattga gaccggtagt
     8521 tgtccgacta atgatgtcac ctacttgatg attgtcaaaa aacgacatcg gtaatgtttt
     8581 gaattttttt gtgatttctt ttcgcaccct caatgtgatc ttttcgctta acgctgccat
     8641 tgtccgttcc tgaataaatg aagtgatgct gcttaagact gaacagccga tcagcaataa
     8701 gacagggaac aacaatgctt gtttgatatc actgaaagaa agctgtccaa tacctgtttt
     8761 tcttatttct gataagagat catcgatccc gattcccatg atcaagggca tggctacgac
     8821 taataggttc ccgaataaac tgcatagaaa caatccgtaa aaatatttat gttcaggttt
     8881 gatcattttc caaaaacgta tcaaagcacg cgtgttcaac gtttttttct tttccatcgt
     8941 atcccttctt tctatattcc ttgtgatttt gcaaactcct gataagtttg gcaattagac
     9001 agtagttctt catgagtacc ttgtccaacg atttttcctt catctaaaac aatgatgtta
     9061 tctgcttgtt ggattgtact gaggcgttga gcaacaatca cgagagtttt ctccgacatg
     9121 tgtttatgaa gggctgctct taatgctgca tctgttttat agtctaaagc ggagaaacta
     9181 tcatcgaata catagatctc tgctggtttg atcaacgctc gagcaataca tatccgctgt
     9241 ttctggcccc ctgaataatt agaaccgcct tgagcaacaa agctgtccaa tccgtcagga
     9301 agggtgtcca ctagttcttc taattgagcg atcttgatcg ctttagacaa ctcttctgtt
     9361 gaggcatatt catttcccat ctgcaagttg cttaaaattg tcccgctgaa taagaaagcc
     9421 ttctgaggga cataactgat gtgggagcgg atctgttctt gagtcatttg agtaagagga
     9481 ataccaccaa gggttacttt tcctatcgta gggtcactaa gtctcaagag aagtttcgcc
     9541 aaactacttt ttccagatcc ggtcccgcca acgatcgctg ttgtttttcc tttaggtatt
     9601 gtgaaatcaa tatcttctag aactggatca gagatttcag tatatttaaa tgtgacatgt
     9661 tcaaaagcag ctgcatttgt ctgatcaaag gttggttcag ctactgtttt gatcgtctct
     9721 tcctcaattt cgatctctgc ttgcagtatt tcttctatcc gtcgtaatga ggcaaacgat
     9781 ttagggataa tgaccaatac catagctgcg atcatcaagt aagcaagtgt cagcatagag
     9841 tattcgacta cagcagtcac gacaccgatt tgtaatgctc cttctgctac aagatttccg
     9901 ccaaaccaca aaatagaagc ataaacgatt cccatgagca aaaaagcaat tggagtgacc
     9961 caagcaaata ttcggttcac tttgatcatt tgattagcat agtcagcaaa gctcttattc
    10021 gttcgctttt cttcatatga ttgattatca aaggcacgga tcatattaat cccagtaaag
    10081 aattctctca atgtgatggt gatccgatcc atttttggct ggatactcaa agataaaggt
    10141 gcccctcgtt tcatcagtcg ataaacaatc catacataaa aaacaacaga gatcaatgta
    10201 acccatgtta gtaatagaga ataacgaaac atcatgagaa tggtgaaaac gcaaataacc
    10261 ggtgctggta agatcatttg gcaaaataaa acaatcattt gctggacatt atcgacatca
    10321 ttcgtcattc ttgtcaaaag agatccagaa ctaaaacgac tgatatcctt cattgacagc
    10381 tgttggaatt tacggaaata tttctcccgc gtttggaatc caaacctagc ggaagccaat
    10441 gctgaaaagt aactagaggc aactgcagcg attgttccta gaaacgctac aagcagcatt
    10501 tcaactccaa ttttttttat aactgaggct tcgccacttg ctatacctgt gtcgatcagc
    10561 tttgctacta attttggtac acctaatgta ccggcgattt ctaaacagat catcacaaag
    10621 gtaaggagag cgagtccttt gtttttctgt aagaatgttt ttaagataga catattattc
    10681 ctcctttttt cctgatactt ttcgattcta taccttcaag taacttgaag gtcaatagaa
    10741 aaaagaataa actgaaaaag aggtgagaaa aaatgaaaaa aatgtatacg attggccaag
    10801 tatcggaact atttgatttg ccaaaatcaa cgatccgcta ttgggatgaa caaggattaa
    10861 tccgttcaag tagacaagaa gagaatgatt atcgattgtt tgatatcgat gatattttta
    10921 tgctttatga tatcgttttt tatagaaaat tagatatacc gatcaaacag atgaagaatt
    10981 tatatggaaa aacattgacg gagttatatg agacacttga tgaaacagaa agaagaatcc
    11041 ataaagaact ggtggtgatg aaacaaaaac aaaaagagat aagggagcaa aaaaaacagt
    11101 tgaaattgat gatcgataca aacgaggaag aatttcctgt agaagagatc ccttttgact
    11161 gtatgatctc aacggagttt gaagatattg tggaaatcaa aaaatttcta cctaactatt
    11221 caagctttgg gatgatgagt atgccagctt ccagttcaca gacgatgtat ggatttttta
    11281 tcgaccctag tgaagtacat ttatttactc aagacgtgat ttgggagaaa aaagatacgg
    11341 ctgtttatcg tcgattctta ttgaaatccg agatgaacca tgcggaacgt aataatattt
    11401 tagaaatcag agaaagaatg tatgaaaaag gatggaaaac aggagaagtg atcggccagt
    11461 atctattgac gaatacggat gaaaacaata tcagaactga atattatcat gcatggatag
    11521 aaatgaaaaa ataggaaata tgtgtttaag ataactcgaa aatgatggtt gttttagtta
    11581 taaagaaaaa ttttctggtt cgattacggg tttaatgtgg gggattatta gtcgtggata
    11641 gatagaattt tagtttttta gaggaaatat gtctttaaaa atggaatgg
//
LOCUS       DACDMM010000078        11669 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDMM010000078 DACDMM010000000
VERSION     DACDMM010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.079"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(37..216)
                     /locus_tag="IWD16_001702"
     CDS             complement(37..216)
                     /locus_tag="IWD16_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088737.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="IWD16_001703"
     CDS             complement(597..1304)
                     /locus_tag="IWD16_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088738.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="IWD16_001704"
     CDS             complement(1297..1734)
                     /locus_tag="IWD16_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088739.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="IWD16_001705"
     CDS             complement(1749..2000)
                     /locus_tag="IWD16_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088740.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="IWD16_001706"
     CDS             complement(2000..2206)
                     /locus_tag="IWD16_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9088741.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="IWD16_001707"
     CDS             complement(2643..2903)
                     /locus_tag="IWD16_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088742.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="IWD16_001708"
     CDS             2978..3181
                     /locus_tag="IWD16_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9088743.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IWD16_001709"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IWD16_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9088744.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="IWD16_001710"
     CDS             complement(3810..4709)
                     /locus_tag="IWD16_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9088745.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="IWD16_001711"
     CDS             complement(4722..5315)
                     /locus_tag="IWD16_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP9088746.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IWD16_001712"
     CDS             complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IWD16_001712"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9088747.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="IWD16_001713"
     CDS             complement(7771..9231)
                     /locus_tag="IWD16_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9088748.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="IWD16_001714"
     CDS             complement(9292..11664)
                     /locus_tag="IWD16_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088749.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2433 c         1958 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtccg
//
LOCUS       DACDMM010000079        11668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDMM010000079 DACDMM010000000
VERSION     DACDMM010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.080"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWD16_001715"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWD16_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9088750.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IWD16_001716"
     CDS             complement(322..891)
                     /locus_tag="IWD16_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP9088751.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IWD16_001717"
     CDS             1077..1979
                     /locus_tag="IWD16_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9088752.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWD16_001718"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWD16_001718"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP9088753.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWD16_001719"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWD16_001719"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP9088754.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWD16_001720"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWD16_001720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP9088755.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IWD16_001721"
     CDS             8547..8732
                     /locus_tag="IWD16_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP9088756.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IWD16_001722"
     CDS             complement(8794..9990)
                     /locus_tag="IWD16_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9088757.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IWD16_001723"
     CDS             complement(10160..11596)
                     /locus_tag="IWD16_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9088758.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
BASE COUNT         3450 a         2486 c         2047 g         3685 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaa
//
LOCUS       DACDMM010000080        11394 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDMM010000080 DACDMM010000000
VERSION     DACDMM010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.081"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(475..1323)
                     /locus_tag="IWD16_001724"
     CDS             complement(475..1323)
                     /locus_tag="IWD16_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9088759.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1487..2389
                     /gene="gnd"
                     /locus_tag="IWD16_001725"
     CDS             1487..2389
                     /gene="gnd"
                     /locus_tag="IWD16_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP9088760.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2391..3392
                     /locus_tag="IWD16_001726"
     CDS             2391..3392
                     /locus_tag="IWD16_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9088761.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3389..3673
                     /locus_tag="IWD16_001727"
     CDS             3389..3673
                     /locus_tag="IWD16_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9088762.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3706..4503
                     /locus_tag="IWD16_001728"
     CDS             3706..4503
                     /locus_tag="IWD16_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9088763.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4517..5350
                     /locus_tag="IWD16_001729"
     CDS             4517..5350
                     /locus_tag="IWD16_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9088764.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5368..6225
                     /locus_tag="IWD16_001730"
     CDS             5368..6225
                     /locus_tag="IWD16_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9088765.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6247..6756
                     /locus_tag="IWD16_001731"
     CDS             6247..6756
                     /locus_tag="IWD16_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9088766.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6793..7194
                     /locus_tag="IWD16_001732"
     CDS             6793..7194
                     /locus_tag="IWD16_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088767.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7306..7659
                     /locus_tag="IWD16_001733"
     CDS             7306..7659
                     /locus_tag="IWD16_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088768.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7693..8136
                     /locus_tag="IWD16_001734"
     CDS             7693..8136
                     /locus_tag="IWD16_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9088769.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            8153..9238
                     /gene="uxuA"
                     /locus_tag="IWD16_001735"
     CDS             8153..9238
                     /gene="uxuA"
                     /locus_tag="IWD16_001735"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9088770.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            9231..9944
                     /locus_tag="IWD16_001736"
     CDS             9231..9944
                     /locus_tag="IWD16_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9088771.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            10696..10896
                     /locus_tag="IWD16_001737"
     CDS             10696..10896
                     /locus_tag="IWD16_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9088772.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3858 a         1780 c         2432 g         3324 t
ORIGIN      
        1 acgtctagac caataataga aaatatggga atataataga aaagatttag gataccccca
       61 aaagaatttc cccaaaaatt cgatgtatcc taaacttttt tattttacat aacaatcata
      121 tcaactgtga aataaaatta caaatgataa cctaaaatga ggccaaaagc cagtgttatc
      181 aaggaaatac ttaattcacc aaaatttttt ttaatttttt agaaaataag aaacaaaaat
      241 aataaaaaaa agaaaagata aattttttac tactgtatac taactttgtt atcgcatcta
      301 acaagtaact cgaataatac caatacaacc ttctccaaag atgcgatgac atacgttcta
      361 acccaccgac tattgatgtt ctgtaccacc cttgcagaac caaaaaagcc actaccaaat
      421 atttggtagt ggcttttttt gttttatgga tttttcgctt tttctgttat ggtgctattc
      481 tttgatttca gaaatagtat gtcgaacttt agcgatagaa cgacgtgatt ctttttcgtt
      541 gtaaaacatc aaaatcacat atagagagtc caaaatgctt agttgagaaa tacgggaagc
      601 taaagcttca gatcggaatt ctgtttcttc agaaatagaa ataaaaacga catcgccaag
      661 tttagcaagg gaagaattag cttggctagt aataacgatt gtttttgctc cagtttttct
      721 tacggtttca gcaatatgga ttgtttcttt tgattgccct gtgtgagaaa tcaaaacagc
      781 acagtctttt tccgtcaata aagaagcttc catcaattga atatggtaat ccgtgctgtg
      841 agaagttgcg ataggggaac gcaaaaattt atgataagcg tcagttgcta caatctctga
      901 accgccaaca ccgaaaaaat agacacgttt agattcattg ataatagtag ccgctttttt
      961 tagatccttt tgttgtaaca acttttttgt gtcatttaaa gttgaaatac tagactcgaa
     1021 caccttttgc gccatagtga gctcagtatc tgttttctga atattttcgt ggatagaaat
     1081 agcagataga tcattttctt ggatcaacat ggccatttta aaatctttaa atccattata
     1141 accaagtttt tttgtaaatt gaaaaaaagt agagtcagca atgcccagtt cacttgctaa
     1201 attgctaata ggactatgac ttactttgct tggattttct aaaatataat cggcgatagc
     1261 ctgttctttt gggctgaagt cgttgtacat tgttttgatt tgtagtctta tggagacgct
     1321 catgagaatt cctcattttc ataataagat tatcattttt tccgttctca ttaatgttaa
     1381 agatacaagg agagtaggaa aaaatcaaac aaaaaatatt tttgtaaaat ctatttacaa
     1441 aaaaattttt ttgtataatg agggtataag aaatggaggc gctatcatgg aaattggtgt
     1501 aattggtttg ggaaaaatgg gattgaattt agctttaaac ttaaaagagc accatcacaa
     1561 agtacaagga ctcgacattt caagtacagc tgttagtcag gcaagagaaa atgacattcc
     1621 tgcttatcaa gagatctctg aatttctagc tacttttgag cagaaaaaaa tcatctggtt
     1681 aatgcttcct gctggagaga taaccgaaaa catgttaaga aaactttttc caaaactcgg
     1741 accaggagac attattattg aaggcggaaa ctcttattat aaagattcaa tccgtcgagc
     1801 agaagagttt cagaaaaatg aaatcggtta cttggattgt gggacttctg gaggaatcga
     1861 aggagcacga catcaagcct gcttaatgat tggaggagat aaagaaactt ttctatccgt
     1921 ggaacaatta ttcaaagatg tagcaattga aagaggatat ctgtatgctg gtgcatccgg
     1981 aagcggacac ttcttgaaaa tgatccataa tggaatcgaa tatggaatga tgcaagcaat
     2041 cggtgaagga ttccaacttg tacaaaaaag tgagtatgct tttgacttgg aacaagtcgc
     2101 acgtgtttgg aatcatggtt cggtgattcg cggctggctg atggaaatca tggaagatca
     2161 gttaaacgaa catcccaatt taagtaatta tcggggaatc gtttctgcat caggtgaagc
     2221 aaaatggacg gttgaaacag cgctagaaat ggctgttcca gttccagtaa ttgcaactag
     2281 tttgttcatg aggaatcttt cacaggaaga agatagtttt tcagcaaaag tagtttcagc
     2341 actaagaaat gggtttggcg gacatgaaat agttgaaaag ggtgagtaaa atgccagatt
     2401 atcatatagc aatcgttaat tccagcagtt tcggaagagt ctttccacac cacttggaaa
     2461 gattaaagtc agttggagac gttcatttct ttactgttga tcaagagatt ccaggaaaag
     2521 aactagcgga gttacttaaa ggattcaata tcattattgc aagtgttacg ccattttttc
     2581 gacgagattt ttttgaaaac aaagatgaac tgctattgat ttcccgacat ggtattggtt
     2641 acaacaatat tgatttaaaa gcagcaaaag aacaccagac aatcgtatct atcatacctg
     2701 ctttagttga acgaaatgct gtagcagaaa ataatataac taatttgttg tccatgcttc
     2761 gacgcacgac acaagcacaa gcaagagtca aggacgatcg atgggaagat cgggcggagt
     2821 tcgtcggtag gacgctgttt aacaaaacag ttggtgtgat tggtgcagga aataccggaa
     2881 gcggagtaat cgaaattttg cgtaacggtt ttcgctgtga tgttctagct tatgatcctt
     2941 acaaatcaga tttatacatc gaaagctttg gtgcaaaaaa agtagaactt caagaattac
     3001 tggaacaatc agatatcatt tgtttatgtg cgaatttgac agaagagaac tatcacatga
     3061 tttctgataa agaagtcgat cagatgaaag aaaatgttta tctatctaat agtgcaagag
     3121 gcgctctagt tgatgaagaa gcgatgatta atggtctgaa aagcggaaag atcgcaggat
     3181 atgccacaga tgttttagaa gaagaaccgg gacgtcaaga tcatccgtat ttagcgtttg
     3241 aacaagtagt gattacgcca catatatcgg cctacacaag ggaatgttta gaagcaatgg
     3301 gtgaaaaatg tgtcagagat gtcgaacaga tcagtcagca ccttcttcct gaacgttctg
     3361 tccagacaaa gagcaactat ataaaaggat gatgaagaaa atggtgaacg gtttaaaagt
     3421 gaaaacaggc cctcaatttt atttatatga agaaggtggt atttcaaaag tttctgatct
     3481 cttgaaatca tacggcgcga aaagagtact ggtcacccac ggaacggtat catgggaaaa
     3541 ggcgctacct aaattagtat ttttgaatga cgaaacgatt caattttttt atcatcgtta
     3601 ttctggagaa tgtagttatg ctgaagccag acgaatagct acaatcatca aaaaaatgaa
     3661 atcgatttcg tagttggcgt tggcggagga aaactgaccg atctagtggg ttatagtgcc
     3721 aatcttgcaa gaatcgattt cggagtcatc cctactttag ctagtaattg tgctccatgg
     3781 acacctcttg ctgtcatgta taaggagaat ggacaagcag aagggaaaac agaacacttt
     3841 tacagacaag ctgctttttt tataacagat ccccaattag tgattgattc ccctgttcca
     3901 tatttcattg ccgggttagc agatacacta gcaaaatggt atgaatctga gctcatctta
     3961 gaacaagatt ttttgcaaga tgaatcattt ttgaagctag cacaagatac tgcgaagatc
     4021 tgcaaagaag agattttgaa tcattctgct aaagcaatcc aagatatggc acaaagaaaa
     4081 ctgacatcgg agttcaaaca gctttcagag attatctttg ctgttgctgg tttagttgga
     4141 ggattaggag ataaatacgc aagaaatgtg gcagcacatg ctatgcatga cgctatcagt
     4201 aaatatattc cggaaagcca tcgttttcta catggagaaa aagtagcgta tgggattttc
     4261 tatcaactgg cactagaagg tcgctgggaa aaaatagacg agttatatcc tttgtatcgg
     4321 gaattagaac tgccggtttc tctagaacaa atgggattat ttccgaaaga taaacagata
     4381 atagataaaa tggctgaatt tattgattcg aaagagaaag tgcatttgat tcctatagag
     4441 gtaacacaga aatcactgat acagtcaatg atggaactgg aaggttatat cagaaagatc
     4501 tgaaggagga ggacacatgg aaacattaag tgtagtacaa agtttgttga ttgctttatg
     4561 ggtagccgcg gttatgtccc gctggttagg tggaggagcc acgttgacct tacgtttttc
     4621 tcctttgatg actggattag tcgtcgggtt gatcatgggg gaagttgcac aagcgatgat
     4681 tgttacagcc gctttgcaaa tgatttatat gggggttttt tctccaggag gatcaatgcc
     4741 agctgaacca tctattgcag cagctatcgc agtaccagtc gcattattag gggatctgtc
     4801 gccagaagca gcgattgccg ttgcggtgcc agtcggattg ctcggaagtt acttgtatca
     4861 gtttcgtttt ttcatcaata cttttttagg taaatataca gacaaagctg tagaagattt
     4921 gaatacaaaa gctatggccc gatcaattat tctttatcca acggttgcct catttttatt
     4981 attcgttccg cttgttttca ttgctttata tttaggggcg ccattgatcg ctgatgtaat
     5041 caaagcttta gagggaacag tcgttatcca cattcttgaa gtagtaggtg gagggttagc
     5101 ggcaatcggt atcgcgacaa ctgtatacgt tatcggtcga aaagattatc tggtcttttt
     5161 cttccttgct tattttatga gtgttgtttt gaaatcttta gatattacga tggttactta
     5221 tgccattttt gggattatta tcgctttgat ctttgtccag tcgcaaaaag ggaaacaagt
     5281 acagcctgcc acaagcaata acaattcagc atcttttgat gacgaagatg actatgatga
     5341 cggattttaa tttaggagga atgggaaatg gctgaaataa tggataatca aacaggtgct
     5401 cctgaagaaa tcacgaaaaa agatgtaaca aaagcctatc tgagatggca ttttgcaaac
     5461 gaaattcctc actcttttga acgttacttg gcgccatcac tactatacgg aatgatgcca
     5521 atcttgaaaa aactttataa agatgaagac tctttaaaag cagcatataa acggcagtta
     5581 ctgttcttca acactcaatt aagctggggt ggcggtgtga ttactggcct aatgtcttct
     5641 atggaacagc aaagagcaga agaagagtat gaagaaaaag agatcttgat gcaggatgac
     5701 ttgatgtata acacgaaagc gggattgatg ggggcactag caggaatcgg agatgcaatc
     5761 gattcaggaa ccgtccaata tatttttatc gcaatcgcgg taccttgggc acaacaggga
     5821 agtcctttag gcgcactttt tccttttatc tgctttgggt tgtatcaggt tttactaggg
     5881 gtatttttcg caagacaagc tttcactatg ggccgtaatg ctacaggcct aaagcaaaac
     5941 actggtgttc agacagcgat tgaaatgcta tcagttttag ggttgtttat gatgggggtg
     6001 cttgcgggaa actatgtaaa agtagaatct tcattgaaat ttgcgatatc cggacgtgag
     6061 tttgttatcc aggaaattct tgatcaaatc gtgccaggga tattaccttt agctgtcgta
     6121 atgggtgtgt acttgttcta tacgaaaaaa ggattgaaag tcacacaagc attattatgg
     6181 ctgactggaa tattaattgt gctagcagga atcggtattt tataaaaaga agggaacgtg
     6241 aataagatgg gtgtagtcaa tttagcgcgt gtggatgagc gtttgatcca tgggcaagta
     6301 atggtaacgt tatctcaaaa aagtggagtg aattccattt ttgttgtaga cgaagttgta
     6361 gcaaaagata aatttatgag agatctttac aaaaatgcag gaagccgtac aggacaaaaa
     6421 acaattgtca ttacaccaga gaaagctaaa ttctattggg atgaatatca atttaaagaa
     6481 tacaattgta ttttgatcgc aaaaacagtc caaacgattt acgacttagt aaaacacgga
     6541 atccccatgg aagaattgaa tattggaggg attgctcaaa aaaatcctga tacagatctt
     6601 ttagtgacga aatctgttta tttaaataaa tcagatgctg aaaaattgaa agaaatgaat
     6661 gaacaatatg gcgtaaagaa tatttatttt caagccactc catctgcacc taaaacagga
     6721 ttaaaagatg tattgaacaa gtttgatttg tcataaacaa atgcttagaa aaaagataaa
     6781 gagggcgaaa aaatgaagaa gaacgagctg tttcgtgatt gggaatttcg ttatcgctat
     6841 atttaccgta agcgccgtac aaaaaagagt aaacagcgtt ttctttcagc attagtatca
     6901 gatatttaca gtatgagaac agatgtgaca gtcattgctt acgatacgct agcctaccga
     6961 tcaaaaaata tttatgttgg agatatcgaa aaagcagaga aagttatctg tacttattac
     7021 gatacacccg ttcatgcatt aggaagttat ttcatgtttg attggaaaga ccaaaggaaa
     7081 aaaacaatct actctatatt attgtccttc attttgttgt tcagtttagg ttggtgggga
     7141 atgatgatat ataacaaaaa tcctcatcac gtattcgatc tgctatccgt ataaacaggt
     7201 atcactgtac tagcatttgg gagctacttt ttcttattgg gaaaagcagc tcgcggatgg
     7261 cctagcagac aaacttttat tcgaaatact tcatccatat taacgatgct agaaatgatc
     7321 cgtacgattg atgatccgag tgtagcgtat gcgtttgttg atgaaggctg ttatggagaa
     7381 aagggcttag acagcgtgag atcgggtatg aaaaaagagg ctattctttt ttatctagac
     7441 tctgttgggg cagatacacc gctacagttt tcgggaaatt atttttcaaa taaagaacag
     7501 tggctgaaac aagttgataa gttgaaagaa aaaaacgtca attatatttt tagtgcaaga
     7561 aaaaagcaag cgcagttctt ctatttgacc aaaacagatt tacgagggaa aacatttaat
     7621 tggcaaaatg ccaatcagat tattgctctt tttcgataag taaaaagtgc aaaacaagga
     7681 gtgagaagac taatgttgga aatcatgatc gttacacatg gtaagttgag cgaaggactg
     7741 aaagatgcgg cggaagtaat tgtcgggaca tcgagccata ttgaaacaat gtgcttagag
     7801 tctggggagg acatccaaac attcggagag aaggtcaaag aaaaattaac tatgatcaac
     7861 acagcagaac ccatgctagt atttgttgat ctaattagcg caagtccgta caatcaagcg
     7921 ctcttggcag tcaatgaact gactgaagaa aagaaagcag ccacttatgt catagggggc
     7981 gtgaatctgc caatgttgtt agaagcaatc aatcatcaga tgcttcacac accgatagaa
     8041 gaagcggtgg aacaaattgt caaacaagga aaagataatt taactgtttg gcatgcttca
     8101 gcggaagaaa aaaaattaga tgaggacgat ttttagaagg aaggctgaaa agatgaagtg
     8161 gggatttaga tggtatggtg aaaaagggga ttcgatccca ttaaaatatg ttcgccaaat
     8221 accgggaata aatgggataa ttggaacttt gctgactaaa atgcctggag atgtttggga
     8281 gatttcagaa attgaggagt tgaaagcatc tgttgagagc caaggactgg aactgcttgg
     8341 aattgaaagt gtagcgatcc acgatgcaat caaggcaggg acagatgaac gagattacta
     8401 tattgataac tatatacgaa caatcaaaaa cttgtctcga tgtggggttc gattgatttg
     8461 ttatagcttc aaaccaattt ttggatgggc gaaaactgat ttgtttaatg aaaacaacga
     8521 tggaagctat tctcttttat acgatcagaa aatcgtcgat cagatggagc caggagaaat
     8581 gtatcaattg atacacagcc agtcaaaggg attcaaattg ccgggttggg aagaagaacg
     8641 gttgaaaaaa ttcgctcagc tggaaaaact gtatgaagga atgaccgaag aagaattatt
     8701 tgaaaatctg acttattttt tagaaaaagt gattccagtg tgcgaaaaag cagatgtaaa
     8761 aatggctatc catccagatg atccgccatg ggaacttttt ggttttccta gagtgacgaa
     8821 aaatcttggc gacttgaaga aaattctaaa tgcagtagat tctccatata atggactaac
     8881 gctgtgcaca ggatctttag gggcaaatcc ggaaaacgat ttagttgaaa tcatccatga
     8941 ggtaggttcg cgcatccatt ttgtccactt cagaaacgtg gagtttttag gagaaagaaa
     9001 gttcaaagaa acggcacatc cctctacaga aggctccctt gatatgtatc atattatgaa
     9061 agcgttggtg caagaagggt tcgacggtcc tatacgccct gatcatggta gaaccgtgtg
     9121 gggtgaagtg gctatgcctg gatatggcct gtatgatcgt gcaatgggac tcacttacat
     9181 gcaaggtcta tatgaggcga taagtaagga aaaaccactg ggagagcatt atgaataaaa
     9241 tcaagctctt catctttgat atggatggcc ttttattaga aactggacgt ttagcttatc
     9301 gtgcttatgt aaaatcagca caaaagtatg attatgaaat gcgtaaggaa gtctattatt
     9361 tactaactgg ccaaacagaa atggcgataa gagaacaaat gggcattttg tacggagaag
     9421 atgttccata tatcaaatgg cgagaagcaa tcaatcaata taaagaaaaa atcgtgaaag
     9481 aagataagag agtatatacg aaaaaaggag cagaagaaat tttatctttt gcaaaagaaa
     9541 gaggaatcca tacaatcgtg gcttcgtcta atacacgggt aaaagtagaa atgtatttaa
     9601 aaatggaaaa tctatatgcc ttgtttgatc aaatcatttc aggggatgat gtaaaaaaag
     9661 gaaagccgga accagagatc tttttgaaag cttgttcaaa aatgaatatt cctccaagtg
     9721 aagcacttgt atttgaagac tcgattgcag ggatcgaagc agcaaggcga gcgggcattt
     9781 tgtcttttct catcaaagat catttgatag ggttgcccga tcataatggg aagcataagt
     9841 tgaaagtaga tgtaagttta tatgaatcca ataaagcaga ctatgaattt tatgacctgc
     9901 atcaagcaaa acgattttta gaaagtaaag ggttgtatct ctaataaaga ggactatttt
     9961 ctagtcctct ttatttattg tcctgcacga ctgagggaac agatgaaaaa gaagtaattt
    10021 tttattttga agtatctacc ttatttgtag tgaacaattt agaacaaaca caaaacgcat
    10081 ctactgttat ctaccagtag atgcgtttaa agagaagaac gagaatcgtc catatgaggg
    10141 tttccactcc ttatgcagat ttttttaatt cttgcactgc ttgttcgatc gtaacacctg
    10201 tcgctacatg attggtattt tctttgtcaa aaacgacaaa tagattcaaa tctgtatcca
    10261 aggttactcg atattgtata tctttgttat agaatgtcat agccattctc ctttctttaa
    10321 atacttgctg aaaggcaacg aacgcgttga taacgaaatt ccatttataa gtataccacc
    10381 aattgaaagg ttttcatagt taagaaattt taaagtcaaa agttgcagat aaaagcaaat
    10441 aaacaagaaa acaaaaagtg aatcaatcgc ggttattcaa ctttttcttc gattcgaaga
    10501 caatagaaga caatgaattt tatatttcat tgcattatcc ttgtgttatt agaaaaagca
    10561 tgatatatta aatgagtaat ctattttgaa acgtaatttg agcgatatat ctacatagat
    10621 aaaactcctt ttgcctatca aatatttatt gcaacgaaaa aacgtacatt gatacgtatc
    10681 aggaggaatt tatttatgaa taacggtaca gtaaaatggt tcaacgcaga caaaggtttt
    10741 ggctttatca ctggtgaaga tggaaatgat gtatttgcac atttttcagc tatccaaggt
    10801 gacggcttca aaactttaga agaaggccaa gcagtgtctt acgatgttga agatggtcaa
    10861 cgtggccctc aagcaacaaa cattgttaaa gcttaataaa aattgacttt taaccgtctt
    10921 ttcgaaagaa aagacggttt ttttgtacga aaaaattcat tggtcgttga atgggctgaa
    10981 tagtagtgta taaaacaatt ttgtggagcg tgaagctttt atggtagagg aaaatacgaa
    11041 taaagtgatg ttgataaaaa gattcctatc ttgaatatga atcgagatgt cttgaaaatg
    11101 aatgagtttg accaatcatt atcctatcga aatttacagt ttcgttcgat tggtctgtca
    11161 attaatgaaa aagtaaaaaa gaatatgttc taggaagagg gcggcacatc taaattttta
    11221 agtaacggac agttcaaata tcaagcattg aaagaggcta tcctactgtt tgaaggaata
    11281 cgaatatata aaaacgacaa agaggaaaaa gtagttcatt aaatcccttc aagtgatgaa
    11341 agaatgagat ctaataaaac ctgatctttc atctgttttt ttaataacgt aata
//
LOCUS       DACDMM010000081        11219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDMM010000081 DACDMM010000000
VERSION     DACDMM010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.082"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            181..1440
                     /gene="lysA"
                     /locus_tag="IWD16_001738"
     CDS             181..1440
                     /gene="lysA"
                     /locus_tag="IWD16_001738"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP9088773.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1427..2227
                     /gene="dapF"
                     /locus_tag="IWD16_001739"
     CDS             1427..2227
                     /gene="dapF"
                     /locus_tag="IWD16_001739"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP9088774.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2230..3111
                     /locus_tag="IWD16_001740"
     CDS             2230..3111
                     /locus_tag="IWD16_001740"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP9088775.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3108..3824
                     /locus_tag="IWD16_001741"
     CDS             3108..3824
                     /locus_tag="IWD16_001741"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP9088776.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3830..4525
                     /gene="dapD"
                     /locus_tag="IWD16_001742"
     CDS             3830..4525
                     /gene="dapD"
                     /locus_tag="IWD16_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP9088777.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4541..5542
                     /locus_tag="IWD16_001743"
     CDS             4541..5542
                     /locus_tag="IWD16_001743"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP9088778.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5539..6900
                     /locus_tag="IWD16_001744"
     CDS             5539..6900
                     /locus_tag="IWD16_001744"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP9088779.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6860..8011
                     /locus_tag="IWD16_001745"
     CDS             6860..8011
                     /locus_tag="IWD16_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP9088780.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7986..9155
                     /locus_tag="IWD16_001746"
     CDS             7986..9155
                     /locus_tag="IWD16_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9088781.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9330..10709
                     /locus_tag="IWD16_001747"
     CDS             9330..10709
                     /locus_tag="IWD16_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9088782.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10990..11219
                     /locus_tag="IWD16_001748"
                     /pseudo
     CDS             10990..11219
                     /locus_tag="IWD16_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3733 a         1956 c         2503 g         3027 t
ORIGIN      
        1 cttctaatag ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat
       61 atttcgaaat atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca
      121 aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa
      181 atgacagaat tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca
      241 cctctgtatg tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc
      301 tttgtatcga aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt
      361 gagatgctgc atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa
      421 atctataccg ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac
      481 aagacgccag aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat
      541 aatttgatgg aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt
      601 atcatgcttc ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg
      661 catattgatt caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag
      721 ttgataaaag aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa
      781 atttttgatg ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat
      841 tttgaccaac ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat
      901 gatcagccga tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct
      961 ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt
     1021 ggagaagctg gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa
     1081 tattactttg tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa
     1141 tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca
     1201 ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa
     1261 tcgggtgatc ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac
     1321 tacaatcgag cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt
     1381 gttcgcagac aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa
     1441 atataatggc tgcggaaatg attttatctt attagattac gcagaagaca cagattatag
     1501 tcaattagcc gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt
     1561 aaaaatcgat ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg
     1621 cggaaatggc attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca
     1681 ggaattcaac gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc
     1741 tttttattgc agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa
     1801 tcaaacttat ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac
     1861 tagtttattt ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt
     1921 ctggagaaaa agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt
     1981 caactttgta gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt
     2041 aggttggaca cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac
     2101 tgggaaaatt tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc
     2161 tggtgaggat gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga
     2221 ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga
     2281 tcagtagatt atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat
     2341 ggattattag ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag
     2401 cagatccttc aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct
     2461 ggaacgaata atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca
     2521 gacgcacttt tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct
     2581 cattttacag caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct
     2641 agaacaggca tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc
     2701 attggtttga aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt
     2761 ggagaagaat tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta
     2821 ggaggaagcg gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta
     2881 gtaaaagata cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt
     2941 gaactgatcc atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca
     3001 ttattagact taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa
     3061 aagaaacaat tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac
     3121 tgattggcag tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata
     3181 cggtccttct tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc
     3241 cagaactgct tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct
     3301 tatcatatca acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag
     3361 aaataaaaga acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca
     3421 tcatggtgat gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg
     3481 aactgattga aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc
     3541 tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa
     3601 aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac
     3661 atgatgtatt gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta
     3721 atcgaatctt tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag
     3781 gttattacaa attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca
     3841 agaaatcatc cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa
     3901 tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt
     3961 tgtttttggt gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat
     4021 tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa
     4081 aattaatgct cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa
     4141 tgccgtgatc atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat
     4201 gattgatatg ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg
     4261 tgcaggagct gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga
     4321 agacggcgta ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga
     4381 tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg
     4441 tggaatacct gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt
     4501 gattgctgcg ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg
     4561 tattgtgggg gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga
     4621 ttttccagta ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt
     4681 attcaagaaa aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga
     4741 tttagcgcta ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag
     4801 tcagggacta tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt
     4861 aatcgttcca gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa
     4921 ttgttcaacc attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca
     4981 gcgagtcaat tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga
     5041 tcttcatcgt acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac
     5101 ggtgattccg gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat
     5161 gataaatgag acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt
     5221 acgtgtgcca atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt
     5281 tacgacggaa gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga
     5341 tctagcagca cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt
     5401 tgggaggatc agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga
     5461 taacatacgc aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa
     5521 aatagcggag gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg
     5581 atgtctcatg aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc
     5641 caagtgatcg ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac
     5701 ttactttatt tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa
     5761 aggatttgtg atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa
     5821 gatgaactag cacgcataca gaaacaactg gaagacgaaa agataactga agattattta
     5881 gtaagtagag gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt
     5941 attgacgcaa aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca
     6001 gcacaggaaa tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac
     6061 ggggcgaacc ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc
     6121 gcgatactgg cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga
     6181 atcatgatgg ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt
     6241 agagaactga gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc
     6301 ccagttcaag aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa
     6361 gggacaaaaa tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga
     6421 cgcaaggatt ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt
     6481 atctggaaag caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca
     6541 gggatcgaca atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc
     6601 atcacgaaag aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta
     6661 gctttgatct ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg
     6721 ttatcgatat tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag
     6781 ttgaatctga tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat
     6841 gaagggatgg tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga
     6901 aatcgggttt catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg
     6961 ggccgttatc catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga
     7021 agaaacgatt gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct
     7081 ctcttttgct tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc
     7141 gatgctttta gggttgactg actttataac taaacacatt gaggatctta tttataatgt
     7201 agtactgatc tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc
     7261 aggattgttg gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact
     7321 agctaaggga gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga
     7381 cattatcatt aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct
     7441 ggaggcagct gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga
     7501 gtggaatcat ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc
     7561 tggagaagtt attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt
     7621 gaagagggga ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg
     7681 ctataatgac ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga
     7741 ttcaaacaaa ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata
     7801 tacgcaacaa tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca
     7861 tgatccgacg ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac
     7921 gattcttttt acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt
     7981 aaataatgat gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa
     8041 aaatacgaca attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg
     8101 gagaaccgga ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag
     8161 aagacttttc tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta
     8221 tatttcagca acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag
     8281 ttggtgcaac ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg
     8341 ttcttattcc agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg
     8401 aattgatccc gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac
     8461 gagcatttga acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc
     8521 ctactgggac gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat
     8581 atccggtatt tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata
     8641 tgtccatagc tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac
     8701 atgcgatgac tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac
     8761 taattaaagt ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg
     8821 ctgaagcgct aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac
     8881 gaagagacta tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg
     8941 gtgcatttta cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt
     9001 gtgtagaatt agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag
     9061 aaggagaagg ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct
     9121 gcaaacggat caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa
     9181 tcgttaattt tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg
     9241 aacgcgtttc ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt
     9301 ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg
     9361 catctcatgg agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag
     9421 atatcacgct atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt
     9481 atttagaaga caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc
     9541 cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa
     9601 aaacagtaac ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat
     9661 tgattttgag ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa
     9721 atgtctactt gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc
     9781 cggctattaa aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag
     9841 caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca
     9901 cttatttaga tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg
     9961 aagttttgac aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca
    10021 aaacaagtga aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa
    10081 atacagaatg gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg
    10141 aatacttgca aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt
    10201 ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac
    10261 gttatgtagt aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag
    10321 gttcctctgc attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta
    10381 cagcagcaaa agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa
    10441 aatatgtacc agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa
    10501 tgacccatca attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa
    10561 atgtattggc actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact
    10621 tcttcttcca gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag
    10681 cgttaggaga agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa
    10741 aaagctgcct tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta
    10801 acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag
    10861 ggactgtgac acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg
    10921 gaggtcggta ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga
    10981 ttatttggat ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa
    11041 atgcttaaaa ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta
    11101 agaaaccgcg gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga
    11161 ataaaagtaa cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACDMM010000082        11180 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDMM010000082 DACDMM010000000
VERSION     DACDMM010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11180
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.083"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            197..1432
                     /locus_tag="IWD16_001749"
     CDS             197..1432
                     /locus_tag="IWD16_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9088783.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2431..2691)
                     /locus_tag="IWD16_001750"
     CDS             complement(2431..2691)
                     /locus_tag="IWD16_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9088784.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2718..4019)
                     /locus_tag="IWD16_001751"
     CDS             complement(2718..4019)
                     /locus_tag="IWD16_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9088785.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4311..4769)
                     /locus_tag="IWD16_001752"
     CDS             complement(4311..4769)
                     /locus_tag="IWD16_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9088786.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(4782..6107)
                     /locus_tag="IWD16_001753"
     CDS             complement(4782..6107)
                     /locus_tag="IWD16_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP9088787.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6124..7536)
                     /locus_tag="IWD16_001754"
     CDS             complement(6124..7536)
                     /locus_tag="IWD16_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9088788.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(7553..8317)
                     /locus_tag="IWD16_001755"
     CDS             complement(7553..8317)
                     /locus_tag="IWD16_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP9088789.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8421..9296)
                     /locus_tag="IWD16_001756"
     CDS             complement(8421..9296)
                     /locus_tag="IWD16_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9088790.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(9551..9635)
                     /locus_tag="IWD16_001757"
     tRNA            complement(9551..9635)
                     /locus_tag="IWD16_001757"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9599..9601),aa:Leu,seq:caa)
     gene            complement(9653..9724)
                     /locus_tag="IWD16_001758"
     tRNA            complement(9653..9724)
                     /locus_tag="IWD16_001758"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9690..9692),aa:Gln,seq:ttg)
     gene            complement(9734..9806)
                     /locus_tag="IWD16_001759"
     tRNA            complement(9734..9806)
                     /locus_tag="IWD16_001759"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9771..9773),aa:His,seq:gtg)
     gene            complement(9868..9952)
                     /locus_tag="IWD16_001760"
     tRNA            complement(9868..9952)
                     /locus_tag="IWD16_001760"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9916..9918),aa:Leu,seq:caa)
     gene            complement(9973..10043)
                     /locus_tag="IWD16_001761"
     tRNA            complement(9973..10043)
                     /locus_tag="IWD16_001761"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10009..10011),aa:Cys,seq:gca)
     gene            complement(10089..10160)
                     /locus_tag="IWD16_001762"
     tRNA            complement(10089..10160)
                     /locus_tag="IWD16_001762"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10126..10128),aa:Gln,seq:ttg)
     gene            complement(10172..10244)
                     /locus_tag="IWD16_001763"
     tRNA            complement(10172..10244)
                     /locus_tag="IWD16_001763"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10209..10211),aa:His,seq:gtg)
     gene            complement(10247..10320)
                     /locus_tag="IWD16_001764"
     tRNA            complement(10247..10320)
                     /locus_tag="IWD16_001764"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10286..10288),aa:Trp,seq:cca)
     gene            complement(10327..10407)
                     /locus_tag="IWD16_001765"
     tRNA            complement(10327..10407)
                     /locus_tag="IWD16_001765"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10371..10373),aa:Tyr,seq:gta)
     gene            complement(10409..10484)
                     /locus_tag="IWD16_001766"
     tRNA            complement(10409..10484)
                     /locus_tag="IWD16_001766"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10449..10451),aa:Phe,seq:gaa)
     gene            complement(10520..10592)
                     /locus_tag="IWD16_001767"
     tRNA            complement(10520..10592)
                     /locus_tag="IWD16_001767"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10557..10559),aa:Asp,seq:gtc)
     gene            complement(10602..10674)
                     /locus_tag="IWD16_001768"
     tRNA            complement(10602..10674)
                     /locus_tag="IWD16_001768"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10639..10641),aa:Val,seq:tac)
     gene            complement(10690..10761)
                     /locus_tag="IWD16_001769"
     tRNA            complement(10690..10761)
                     /locus_tag="IWD16_001769"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10726..10728),aa:Glu,seq:ttc)
     gene            complement(10776..10865)
                     /locus_tag="IWD16_001770"
     tRNA            complement(10776..10865)
                     /locus_tag="IWD16_001770"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10829..10831),aa:Ser,seq:gga)
     gene            complement(10905..10978)
                     /locus_tag="IWD16_001771"
     tRNA            complement(10905..10978)
                     /locus_tag="IWD16_001771"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10942..10944),aa:Asn,seq:gtt)
     gene            complement(10988..11103)
                     /gene="rrf"
                     /locus_tag="IWD16_001772"
     rRNA            complement(10988..11103)
                     /gene="rrf"
                     /locus_tag="IWD16_001772"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         3554 a         2412 c         1815 g         3399 t
ORIGIN      
        1 aaatcgatca aaaaattgtt gcgtgttttt ttgaaaattg gagtttcaac ttgattacaa
       61 aatttaaatt tagaagttgt gaaaacagcg agagacagtt taatattcaa aatatacata
      121 ggctactcga acgctgtgtt ttttagtata ctagtaagaa acgaagggat agactcattc
      181 caataaggag gatttcatga agacaattct ttttgctcct gcagtattca atttagctga
      241 gacaacacga atgattgagg ttgccaaagc tatgaaagac cgttattact gtgtattctt
      301 tggcttttct aaaacttttg ctcctttgat cacagaaagt ggctttgagt atcatttact
      361 agcgccgatc cttacgaaaa aacaagaaga acaaatcata aaatttgacc agttgcgtac
      421 acttaaaaac ccttttacca aagaaatggt tgcctctcgt atcactagtg aacagagtct
      481 gattgctaag tttgagcctc gactgattgt gacaggttcg aatgtgacaa tttttttatc
      541 ggcccgcaat aaaaagattc ctttagtata tgttaaaccc tatgctttga gtgaaccttt
      601 ttttcaatca gagcatgcgc tgatgcccca cgagttacgt ttcttcggcc ctttaagcaa
      661 tcccctttgg cactggacaa aaaagcgtat agtaaatacc cgatggatcc ccaatgcctt
      721 caaacacgct gcacaaaaag aaatgcttcc tttaccaaaa acaagtttac aaatgatgga
      781 tgctgatcta aatctcatca caacgcctaa actatttttg gaagaagaac agctgccgga
      841 aaattatcga atcgttggtc caatttttgc taagctcagt acttccttgc ctgaagaagt
      901 gctaactttt atacagcgaa agcaacaaga aagaaaacgt attatctatt tcgcaatggg
      961 tagttcaggc aatccgcgta tgttacgcaa aattttgttc ttcttacaaa aacaaccgga
     1021 tctagcggtt attgcaccta ttgactatct gcttgatgac attcaatctg actcagaaga
     1081 tcttttaata acagaatatc tgtctagtca tcttttgcat gaattcattg atttttcttt
     1141 tatccacggc ggggaaggga ccgtccaaac tgcctgtgaa tccggcaaac cgtttatcgg
     1201 tgtggggatg caatttgagc aaagctgtaa tattgactat tgtgtaaaat acggaaacgc
     1261 aatcgccctc acaaaaccag tgactgaaag aaaactgcag attgctctgc gcgaagtttc
     1321 ttcacccaag attcgtcagc aggcacgcac gctgcaagcc gtttttccaa taaatggtcc
     1381 acaacaagca attcaagaaa tcgaaacctt cctgagcaac cacccaatat agtttcaagt
     1441 agaaaaagct tttttaatag agactcatca gtaatttcgc tattcgagac ccagacagtg
     1501 aaaatacaaa aaaagtaaat agtggattga aaagttagag ttacttaaat tacggtgtct
     1561 aaaaaacaca aaatcatcct taaaataaaa gatctgacgc tatccaaatg agcataggta
     1621 gcgtctgcca tttattctta gtcaaattct ttgacacctt tctctggttg gatcatctca
     1681 acgtttcttc aaaagaaaga ccataataat taggcaaata cattttctta aaatgtgtca
     1741 tatagatcta gaagcatcgc tttgtagaga tttgtgccgc tagtcccctc aacaattact
     1801 aaatcaaatg taggatttac cactttatct ttgataatca acctccctct tctgttgaga
     1861 caatgatctc gcaatccgat gcatactcct atataaactg ccagcaatcc ccacatattg
     1921 agtagatcat gtcagaatgt tctatgtcgt cagcaacgat acactaaaag tcacgttcac
     1981 catgactgat tgctattccc agcacaatta tttctataca cgtttcatga tttgaataaa
     2041 cactcacatc atacatatta ccattcatac accatactgc agcccctact atatggttgt
     2101 aagctcccaa atctatattt gacttgatca ctttggtagt agatctacta attctaaatc
     2161 cattgctgat aaaatttcca ttttttcctc cttctttctg ccaaaacagt taaaacaata
     2221 ataatcttta tgtaaaaaag ggctgtgaca aaagccttgt cacagtccca tattccgaat
     2281 aaacggtggt caaaagcttg catctgcggt aataagttca accaaacgaa aaataagcca
     2341 acatttcaca aaaatttaag aagcaatttt cgtaaatttc ttcttatttc ttgaagctaa
     2401 ccacttctgt tacaaccact tttagaacat ttaaagcttc gatgtgacaa accatctatg
     2461 ctgctcgatc ataaaattga tagcaaaaat tccacaaagt acatatgtcc cccatgaaaa
     2521 caaaacattc atatgcggta caatgatctt tggaagaatc acgatcaaaa atgatgccag
     2581 tgcccaaatc catcgattga tttgaatatg atatgtagct agcatattca atataaggtg
     2641 actagccgtt aataaaaaga taaaaacaaa aaataaaatc agccaataca ttgtttccca
     2701 ccttctaaaa accaaactta gacagtctgg acttcgtttt ctccacgact ttgttcatct
     2761 gacagcatct ttttatcata agcttttaca aatggataat agataataaa tgctatcaat
     2821 gtgttgatca tgatcaagat aacaacacgc cagtcatttc ctgctgaaag ataaccgccg
     2881 attggagctg gtaatgtcca tgttggcaaa acagtaaatc tattgaccat ttctaatttg
     2941 actgcaaaat aactgataat agtcaaaact aaaggaacta agttgaacgg gatgaacatg
     3001 taaggattca acatcatagg agctccaaaa attatgggct cattgatatt gaagatacca
     3061 ggaataatag aaaaacgacc aacctctttc aagaattttg atttacagaa gaatataaac
     3121 aaaacagata ccacgatcgt tgctcctgca ccaccaattg ttaccgtcca ttgataaaac
     3181 tgttcaggag cgatataggg taattgggtg gcagctgtac catctgccaa tgcctttgca
     3241 ttctcatcca acatgatcag ccacattgga cgaacaacag cccctacaat actcatgcca
     3301 tggataccaa atgaccacaa caaatggatc aaaaacattg gtagtaaaac accaagcaaa
     3361 ttattcccta aaatattggt taaaggctcg aataacgata ataaggcagc gtttacatca
     3421 aagcccgcga cgcttcgaat cagccaagta attgttaaaa tgactgcacc tggaatcaat
     3481 gcctcaaaac tacgggcaac agcaggtggc acttgttctg gcattttgat agtaatattt
     3541 cgatttttaa agaaacgata tacttccact gcaaaaatca tagataagat ccccgcaaac
     3601 atcccagatg ctcccaaaga actcataggt aagacccagc caagaggtgc ctcttcagga
     3661 gttaaaacgt ttacaggaac ttgtaacata aagaacgttc ctaaagataa tataccacct
     3721 gtcacactat ctaatccata tgatttagct agatccgaac ccataacaaa agttgcataa
     3781 atagccatca atcccatcgt catcctaaag ggtaaaacaa tcgttgccga ataaggggca
     3841 ataaattcat tccatccagg aaacggtaaa ttgataaaga ccataaaaaa tgtcccaatc
     3901 aaaatcaatg acaatgttga aacaacccca ttacgaattg ctaataaatg acgctgtgta
     3961 ccaatttttg ccattggtgg gacaagtttc tcttcgaccc atttcatcag cttttccact
     4021 aaaaccactc cttttcaata aaatatgaac cattcattgc atgacaacgc aaagaatgtt
     4081 cataactaat tatataatta agaaatttta tttcaatata ttctcttaaa taatcaaatt
     4141 tatttttaag aatcctgaaa ataagttttc taaatcatag gagcaacaaa aaaattcgat
     4201 aattaacaaa aatcaaaatg attaaatcta gtgattgaaa catatcattt caaaaaaatt
     4261 ttgtaaattc acccataaat atcaccctat ttaacttttt tcatcgtaca ctaaaattca
     4321 aaacaactat ctttaatctt tacttcttgg ttttgtttta ataaatttcc ttcaaaaatg
     4381 cctggagaaa ctacaatgat caccgtatct ttttgtgctt gctccaactt cttcaaatct
     4441 ataatagcta acagtgtccc ttcttttacc tcctggttac tttcaacccg tatctgaaac
     4501 ggacttcctt cgagttcaac tgtatctaaa cccatatgga tcagtatttt tctgccagat
     4561 tcactttcga tcgttatggc atgtcgggta ggaaatatat tatcgattat tcctttaact
     4621 ggcgcatgga tatatccatc atgattcgtt atagcgtatc caactcctat catttttgaa
     4681 gaaaagacct cgtcttttac tgctgttaat ggaataattt gaccactagc tacagctttt
     4741 agaatgtttc ttgttttaaa gaaatgcact ttttatcacc tctactttaa tgctggccaa
     4801 tattttttgt ttactttgat catttcatct agtatttttt ttgcgactct ggcatttggt
     4861 acggtttgat tcaatgtaaa tgcttgtaaa gctttttgat aagaaccttc aaagtaagca
     4921 tctactagta acttctctac tgcaacttgt gcctccatca atcctttatg aaaatcactg
     4981 attggttttc ttagtgtaat tggctccaca ccagttgcac caacatatgc cggtatttca
     5041 acaactgcat ctgaccgtag attcggaatc gccccttgat taggaacgat tagcataaag
     5101 cgacgacgat catcattcaa caaggaaata gctatatcga cgatatattg tccatgctcc
     5161 ccaaaattaa agttcaatac ttcttccaca ataccgcggc gtattttatc agccatttct
     5221 ttggtatttt tctctcgtcc atccatgacc atattagcac gtgtatactc tttgtcagta
     5281 tgctcaacga ccatatcagg atataagtaa tactctaagt aattattcgg gatattactt
     5341 ggaaaatttt tcgtgatgac tgacatcatt tgaaaggttt tttgccaagt tttatctcct
     5401 atattaaaat cggcgacttg catttctttt gttattagtt tttcaataat ctctggcata
     5461 atttctcgtt gcaattcttt atcataaatc gatgtatacc agccaaagtg gtttaaacca
     5521 taatacgtaa cgatccaatt cttacgatca taaccgtaat tcactgcaat cgtttcttca
     5581 attgaaatag tcatatcaca ggcattaatc atttttacat tcggatattt acgacgaaca
     5641 gcttcagaaa cgatcgactc tggatttgta taattcaaaa tccatgcttt gggggcatag
     5701 tcttgtatat gatcgacaat ttccaataaa cctttgattg agcgcatccc ataagcaaat
     5761 cctccgagtc cacacgtttc ttgtcctact aatccatgtt taagtggaat cttttcgtcc
     5821 ttttctcgca tctccaatcc tccaacacga atttgtgaga aaacaaaatc gcatcctgta
     5881 aaagcaagct ttggatcatc cgttgcacga atgcgaatat ttgaaaaacc ttttcttttc
     5941 agcataaatt taacaatcaa gtacatatcc atatttcttt cttcatcaat atcatataaa
     6001 cgtatttcag ataatggcaa ccgtttttca ttgttcaaaa tggcttgaac gattcctggt
     6061 gtatacgtac tcccgcctcc agctatagtg ataatctgtt ttttcatcat catcttttcc
     6121 tttctattct tctaataatt tattgaattc tgccataaca taagtcactt ctaagccgat
     6181 gataatttga taattttttt cagttggttt aatgatcccg cttatcccta attgcttgat
     6241 tcgctgttcc tcgaccatgg tgggatccac tacctctacc cgtaggcgtg tattacaact
     6301 atagacatcc aggatattgg cttttcctcc aaacgcttgt agcatttttg ttgccatata
     6361 ttcataattt ttacttgcta accctaactc tgtttcttct aaggttactt cattgccaaa
     6421 ctctgcctta gcttcacgtc ctaatgtctg aatgtttctc ttattgatca aataataaaa
     6481 agtaacgtaa tacaaggcaa aaaagaaaat tccgattgga agaatcaaaa tagcattggt
     6541 agcgattcga aaattgatga gataatcaac aatacatgct ccgaatgtaa aaccaattcg
     6601 tatattgaat aagtaacaga caatccctgc taaaccggca aaaagtgcat ggatcacgta
     6661 caacatagga gaaagaaaca tgaaactaaa ttcaacaggc tcagtgatat ttgcaataaa
     6721 tgaagttcct gcaccgcttc ccatgattcc tttgaccatc tctttatttt ctttataagc
     6781 tgctttatag atagctagac atatagctgg tataccaaac atcattacta caaaaaaaac
     6841 tgataaaaac agtccagcct gaggatctcc gttgataaag cgtgtcatct ctcctctgaa
     6901 aacttccttg ctaggagatg tatagcttcc taaatcgtaa taaatatacg tattcaacac
     6961 atggtgcagt ccaaagggga tcagcaaacg attcaaaaac ataaatatcc ccacacctaa
     7021 tgctcccata tcaacgagca gttttgcaaa attatcaatc ccaatttgga tgagcggcca
     7081 gataaagccc aaaaaaacag ctgcgattgt ttgtagaatc atcaccatag tcaatgggaa
     7141 tttttcgccc gcaaaataag aaaaaactac tggtaatttt tgttctttaa agcgattata
     7201 cgtccatgcc gctactaaac cggaaataat tccaccaaaa atccccatat tcacttctgg
     7261 gtccataatt tctaagcctt ttttcaaggt agcaatcgct aaaaaaccag taagtgcagg
     7321 tattcctttg tccttatttt tgacaaaacc gattgcaact cctatagcaa ataagatatc
     7381 caagtttcca aaaacggcat ttccagctgc accaattact gggatgttca gcatatcgtc
     7441 agctgataaa cgcatgatca atcccgctaa tggcatcgct gcaactggaa gtagcatcgc
     7501 tcttccaagc ttctgcacat ttgatttaac attcattttt attcctcctc tattaaatat
     7561 cctcatatgt tactaactca atcaaaggat catttttcac tttttgagca aatgctgaat
     7621 gaattaaaaa ttcgacttgg tgggtacgag gatacacaaa acttgaacca tttaacaatt
     7681 cctgatccaa atacgctgcg tgacacatga cttcgatcgt tttatcttct ctcaatatca
     7741 tgagtaaact tgccaaatat tcttccacac tcgtattgat ttgtcgtttt ttttcatcag
     7801 tttctcctac gacatcgaaa caaggctcaa aataatcgac atgacgaact tcatcttttc
     7861 gttcaaagtt gctccgaacc ggcaaatcat atttttttgc taatgtaaca actacctcct
     7921 gattttcatc caaagtatga gtatgatgat gactgtctaa atgagttggt atgatccccg
     7981 catgataaat tttttgaatc tgagcattcc attcctgata cagttgatct ttgtcaatgt
     8041 taaatggatg ctgataataa gataaggatc tgaactctcc gctttcgata agagtatcga
     8101 cagttttgag gagtggtttg ccacacgtca atgttaaatg gactcctaca cctagcctag
     8161 gcatttcctt tcttagtttc acagcatgct cgaaacccgg catatttgcc atcaatgtag
     8221 ttgatgtcaa aattcctcgc tgataagcat ccataatccc ataattcaca ccatggctat
     8281 agccaaaatc atccgaatta aaaatcactt ttcccatttc attctccttc ttttttctac
     8341 taaaataggc gcaaccaaat aatcatagtc catttactat gtgtcatttg gttgcgccta
     8401 tagatagtta cgcccttatt ttaatagatt cttgctagat gaattgccaa atagatgatt
     8461 tcattttctt gaatggcaag ttgaaattgc tgttcgatga tatctttaat ctttagtgct
     8521 gtacaatacg ctttgctatg tttaaatgtt tcagtaaaaa aaccaatatt ttctactggc
     8581 gtttcttcct gataaatcaa gcgtttaacc aggaatttga tatgaaccat taagcgatga
     8641 taagtcagtg attctttatc ataaacgttt gaataggatt tttctagatg ggacaaaatc
     8701 gtggtcgtca tttctactat ttttaagctc tttaaactat taggctctcc tagctcagca
     8761 ttaatgatat gcatagtgat gaatcctact tcatttgacc ctaactcggt ttgatatcga
     8821 tgattgattc gctcgaccac ttctactgct atatcatatt catttggata aaggaatctc
     8881 atttctggca acataattgc tgtgacatct ctatcttctc tttttcgttg aatagcaaaa
     8941 taaatatgat ctgtcaatgt aatatagata tttgaatcta atggagagga gatcctagct
     9001 gttatcagtt caatcacttc ttccgcaaaa tccataaatt cgattggaat ctgcgagact
     9061 atctcactga atcgtttgat cttttcttta gaatctaata caaataattt gtcagcatcc
     9121 tcaacatcaa atggcttact cactttagca gaaaagccaa tccctttccc catcgcaatt
     9181 ttttctattc catcgtcatc gatcaataca gcattatgat tcagcttttt catcacatac
     9241 accgttaatt cacctcctct atatgataaa acaaaacaac ttgcagactc cttcatacat
     9301 atttttgtct ccaaacaatt tttaaagaca aaaaacgacc taagaaagaa ccctttctta
     9361 ggtcgtgcct aattgaatag tagcacccta cataatttac aaaaatagtt taacacataa
     9421 taaaagcgtt atcaatatct tttttactcg ttttatgata atacttgtat cgaattgatt
     9481 tggaggtaat tacataaaaa caaaaaaaat atagcaactc tctataagaa agttgctata
     9541 ttgataatta taccggcggc cggggtcgaa ccggcacgtc ctcaacggac actggatttt
     9601 gagtccagcg cgtctgccaa ttccgccacg ccggcaaatt taaaaataat aactggggta
     9661 gctggattcg aaccaacgca tgagggagtc aaagtccctt gccttaccgc ttggctatac
     9721 cccaagaata aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
     9781 tgcgttaacc acttcgccac attcgccatc ataaaagatg ttatttaatt tctcaataaa
     9841 aaactaagat gaagacatct tagtcaatac cggcggccgg ggtcgaaccg gcacgccctc
     9901 aacgggcact ggattttgag tccagcgcgt ctgccaattc cgccacgccg gcaagttgat
     9961 ttattagtac taaggcggta accggatttg aaccggtgat gaaggttttg cagacctctg
    10021 ccttaccact tggctatacc gccattatac tattatcaat ctataaaata aagaataaat
    10081 tatagaaact ggggtagctg gattcgaacc aacgcatgag ggagtcaaag tcccttgcct
    10141 taccgcttgg ctatacccca agaataaaaa gggcgaatga tgggaatcga acccacgaat
    10201 gccggagcca caatccggtg cgttaaccac ttcgccacat tcgccatggc aggggcagca
    10261 ggaattgaac ccacactaac ggttttggag accgctgttc tacctttaaa ctatgcccct
    10321 ataaaatgga ggagagtgga ttcgaaccac tgaaccctaa ggaacggatt tacagtccgt
    10381 cgcgtttagc cacttcgcta ctcctccatg gtggcgcggg acagaatcga actgccgaca
    10441 cacggagctt caatccgttg ctctaccaac tgagctaccg cgccatattt taaaattatt
    10501 aattgaataa caagaacgac ggttccgacg ggaatcgaac ccgcgatctc ctgcgtgaca
    10561 ggcaggcatg ttaaccccta caccacggaa ccagttcatt atggaggtta acgggatcga
    10621 accgctgacc ctctgcttgt aaggcagatg ctctcccagc tgagctaaac ctccaacatt
    10681 caattaaaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag ggcggtgtct
    10741 taaccgcttg accaacgggc caggtaaatt tgctacggag agtaagggat tcgaaccctt
    10801 gagacagttt ggcaccatct acatgatttc caatcatgct ccttcggcct ctcggacaac
    10861 tctccagagt gaaagaagct caatactctt tctcaaaatg agaactccgg cagtaggact
    10921 cgaacctacg acatcatgat taacagtcat gcgctactac caactgagct atgccggaat
    10981 aacatcagcg tggcgacgtc ctactctcac aaggggcaac ccctcactac aatcggcgct
    11041 aagaagctta acttctgtgt tcggcatggt tacaggtgta tccttctcgc tatcgccacc
    11101 acactgtggt gttatctttt attgagtaaa ttttgttcac tcaaaactgg atttgaagtc
    11161 acataagttt ttttccgagt
//
LOCUS       DACDMM010000083        10929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDMM010000083 DACDMM010000000
VERSION     DACDMM010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.084"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..288)
                     /locus_tag="IWD16_001773"
     CDS             complement(<1..288)
                     /locus_tag="IWD16_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9088791.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAEN"
     gene            430..1548
                     /locus_tag="IWD16_001774"
     CDS             430..1548
                     /locus_tag="IWD16_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9088792.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1676..3052
                     /locus_tag="IWD16_001775"
     CDS             1676..3052
                     /locus_tag="IWD16_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9088793.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3453..5033
                     /locus_tag="IWD16_001776"
     CDS             3453..5033
                     /locus_tag="IWD16_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP9088794.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5231..5454
                     /locus_tag="IWD16_001777"
                     /pseudo
     CDS             5231..5454
                     /locus_tag="IWD16_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5659..5979)
                     /locus_tag="IWD16_001778"
     CDS             complement(5659..5979)
                     /locus_tag="IWD16_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9088795.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6127..8361)
                     /locus_tag="IWD16_001779"
     CDS             complement(6127..8361)
                     /locus_tag="IWD16_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9088796.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8840..9106
                     /locus_tag="IWD16_001780"
     CDS             8840..9106
                     /locus_tag="IWD16_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP9088797.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9106..10833
                     /gene="ptsP"
                     /locus_tag="IWD16_001781"
     CDS             9106..10833
                     /gene="ptsP"
                     /locus_tag="IWD16_001781"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP9088798.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1912 c         2216 g         3322 t
ORIGIN      
        1 gttttctgct tcttcaatca atatttttcc taacccttgg tgtctgtatt cagaaagaat
       61 cgccatccgc tgtaatttca tttttccgtt ttctaaaggc aaaagacgta cagttccttg
      121 cggttctttt ttatctgagt acaggacgaa gtggatacaa tgggcttcat ctttgtctat
      181 ttctcgagac aaaggcacac cctgttcgac tacaaaaact tgatgcctga tccgtacagc
      241 atctaagtaa atattgctca ttgtatcttt tgtttgtaca actttcatgg tctagccacc
      301 ttcctttcgt atagtatact ggaaaataaa acaaataaaa agtatggcaa gttttcaaaa
      361 ataaaaaaag aataaaagaa tttttaacga atataatata tactagtaag aaagaaagga
      421 ggaaatctat tgtttgaaaa acttagacag tctaaactaa tgttttggtc gcttgaatta
      481 ttgatcatag ctgcattgat catgatttca tcagaaataa actttatttt cgaaccgatc
      541 gggacatttt tttccacgtt atttgcacct gtactgattg caggctttct ttactattta
      601 ttgaatccta ttgtcaagct gttgatgaaa gcaaaaatca aaagaatatg ggctgtcgca
      661 attgtgctgc tccttttagt tgcagcaatt atttggattt tacttagtgt catcccaagt
      721 ctagtacagc aaatttcctc attggcatct aatatgcctg atttcatcaa acaggtagaa
      781 agctggttaa aagaggtagc cgaattgcct ttgttcaagg aagtagatat caataaatat
      841 tttgagcaat tagacatttc ttacgggaca atcattcagc aattccttag tggagtatcc
      901 aatagtttag gttcgatcgt tggaacgatc gcttcggcaa ccgtagtaat cattacagca
      961 ccattcatct tgttctatat gttgaaagac ggagaaaaac tagtgccaaa catccaacag
     1021 tttttcccag aaaaaagaag aaaccaaatt gtggagttat tggggcaact gaataaaaca
     1081 ttggctaatt acattagcgg acaagcaatc gaatgtttat tcgttggtac cttcacattt
     1141 ttaggatata tggtaattgg tgtggattat gctttcttat ttggggtcat tgcgggattg
     1201 acaaacctaa ttccatattt aggaccttat ctaggactag cacctgcatt tctagtaaca
     1261 gtcttcaatg atccggtaaa agcattactt tgttgtgtgg ttgtgctaat cgtccaacag
     1321 ttggatggga atatcattta tcctaatgta attgggaaat cactgaaaat ccatccatta
     1381 acgatcatta tcgttcttct agtagctgga aatattgccg gattgcttgg tatcttctta
     1441 ggtgttcctt tttacgcaat ctgcaagacg atcatttcct atataatcaa aatcgtcaaa
     1501 gaagacaagc aaaatgaaaa caagaagaaa attgctacga cctcgtaggt agttttagta
     1561 cacttgtttt tattgcaaag aaaattgctt tgtgatacca ttttaacgtg gctatagtgc
     1621 cacgtttttt tctatcgtaa actaaaaatt aattttataa ggagagaatg cgattatgaa
     1681 tgctgaccct gagagtcagt cgctgttagc gcagatttta ttattgatcg ttttgacatt
     1741 gatcaatgct tttttagcag cttccgaaat tgctgttgtt tcgatcaata agaatcgaat
     1801 cgagcaaaaa gctgatgaag gagatgtgaa atctcaaaag cttttaaaaa tattgcagaa
     1861 tccaaataat ttcctatcga caatccaagt aggaatcact ttggtcaaca ttttgtctgg
     1921 tgcttcacta gctaatactt tatcggaacg gttagctccg gtattaggtg gcggcacagc
     1981 ggcaaaaaat attgcaaata tcattgtgct ggctattttg acctatgtat cgattgtttt
     2041 tggagaattg taccctaaac gtattgcttt aaataaatca gaagaagtcg caaagttcac
     2101 ttcagggctg atacgcttga ttggtgtagt agctaagccc ttcgtctggc tgctttctgc
     2161 ttccacaagt ttattggcgc gtatcacgcc gatgactttt gatgatgaag attcaaaaat
     2221 gaccagagat gaaatgcgtt atatgcttga aaacgaaggc gtattgaata atgaagaatt
     2281 agaaatgctc caaggggttt tttctcttga tacgaaagtt gcccgagaag tcatggtccc
     2341 acgaacggat gcatttatga ttgatatcaa cgacactgtg gaagaaaatg tgaatgaagt
     2401 tttatccgaa aactattcaa gaatccctgt ttataacgaa gacaaagata aagtagtcgg
     2461 aatccttcat acaaagaatt tactaaaagc tgcccataaa ttcggctttg ataatttaga
     2521 tatcaagaaa atcatgcagg agcctttatt cgttcctgaa acggtcttta ttgatgatct
     2581 gttatatgaa atgaaaaaga cgcagaatca gatggctatt ttattagatg aatatggcgg
     2641 cgtcgttggg ttagcaacac tagaagacct tttagaagaa atcgtcggag aaatcgatga
     2701 tgaatcagat gaagttgaga atctttatga aaaaatcgat gaacacgaat atatcatcca
     2761 aggacgtatg ctgattgatg agttcaatga agcttttgac agtgatctgc atatgagtga
     2821 tgtagatacg atggctggtt atttgattac agcattaggc atgattccgg atgaaggcga
     2881 aaagttatcg tttgatgtcg ataatattac gctggtttct gaagaaatgg aaggttctcg
     2941 aatccttaaa atccgtgtca tttttcatga tccggaagaa acagaagcgg aacctgaaga
     3001 agaacgaaga tacttcagaa aagaattcga agatgacgag ccaagaagat aattggaaac
     3061 ttggttatga aagacgtgtt gcttgaataa acaagcaaca cgtcttttct tttagtcagc
     3121 tgacaaaaaa atatttgact cttatggttt ggtggctttt tcattcgctg aatccatgct
     3181 atactgattc agttgtagta gttgacaaag gttgtgaaaa agctatcctg cattaagtaa
     3241 taagagcagc caagcaaaaa cagcttttcg tatttttggc ttgggtgggc ttattgctga
     3301 taggctgtct ctagaacgcc gtttactcgg aaaaagagac tgctacacgg cttttgtcac
     3361 agccctatat gaaattgtct ttctttaaga gatcggacaa tctgtgccta gagaaaatat
     3421 aaataattga atggaagaaa tggagttagg ttatgaataa tccaaaatta aagcaacaag
     3481 tggacaatcg cagaactttt gcgatcattt cccatccgga tgctgggaaa acaacgatta
     3541 cagaacaatt actattgttt ggtggggcta tccgtcaagc cgggaccgta aaaggcaaaa
     3601 aaacaggtaa ttttgccaaa tcagactgga tggaaatcga gaaacaacgt gggatttctg
     3661 ttactagttc tgttatgcag tttgattatc aagggaaacg agtaaatatc ttggatacac
     3721 caggacatga agatttttct gaagatactt atcgtacttt gatggccgtg gatagtgcgg
     3781 tcatggtaat cgatagtgca aaagggatcg aagcacagac aaagaaatta ttccaagttg
     3841 taaaaaaacg tggcatcccg atatttactt ttatcaacaa actagatcgt gatggtcgtg
     3901 aaccattgga attgttagaa gaattagaag agctattaga tatcgaatct tatccgatga
     3961 attggccaat cggtatgggt aaagggctag aaggtcttta tgatatctac cataatcgtg
     4021 tagagtttta tcgtccggaa aattatcagg atgaacgatt agtagaattg gatgaagatg
     4081 gactgcttcc cgaaaatcat cctctaactg aaaattcctt atatgaacaa gtcttagaag
     4141 aagtagaact aatcaaagag gctggagatg catttaatca agaaaaaatt gctcgtggcg
     4201 atcagacacc agtatttttt ggttctgcat tgactaactt cggtgtacag acttttttag
     4261 agactttcgt tgaattggca ccagctcctt atggacacaa aacacaagat gatcagatgg
     4321 taagtcctta tgaggaagaa ttttcaggtt tcgtattcaa gatccaagca aatatgaatc
     4381 cagcccatcg tgaccgaatc gcttttgtcc gaatctgttc tggaacattc gaacgaggaa
     4441 tggatgtctg gttggaacgt acaaataaaa aactgaaatt aagcaatgtc acacaattta
     4501 tggcagattc gagagaaaac gtcgaaaaag cagtagcagg cgatatcatc ggtgtatatg
     4561 atacaggcaa ttatcagatt ggggatacat tgttcgaagg aaaactaaaa gttgcttatg
     4621 aagaactccc ttcatttact ccagagctgt ttatgaaagt gactgcaaaa aatgtcatga
     4681 aacaaaaatc cttccacaaa ggaatctatc aattagttca agaaggagcg attcagcttt
     4741 ataaaacata tctaacggaa gaatatatca ttggagctgt tggtcagcta caattcgaag
     4801 tattccaata tcgtatgtta aatgagtata acgccgaagt aatcatgtct ccaatgggac
     4861 acaaaatcgc tcgctggatc gatccagaag atttggatga gaagatgagt tcaagtcgca
     4921 atatcttggc tcgtgaccgt ttcgaccaac cgttgttctt attcgagaac caatttgcag
     4981 aacgctggtt tgcggataaa tatcccaatg taaaattaaa aagtttgatg taaagaagtc
     5041 ttgacacaag ctgccctgca tcaagtaata agaatagcca aacaaaaaca gcctctcata
     5101 ttttgtttag agccgtgacg gattttccgt gacggctctt tttactatat taaaatcata
     5161 gaaatgtcta atcaaaaaaa tataaaaatt attttgcgta catatgatta aagaaaggag
     5221 agtgtgacac atggaaacag aatggcaaag acgtgaaaga aaaaaacaga aaaagaagag
     5281 atgttacgat tatgtcgatt attagatgta aaagactggg acaaaaacga gcaaattgca
     5341 caaaaagtac gatcaatcat ccataaagag gaaaaagtta aaaataaaga cacgcgtaga
     5401 actgaaaatg aaaagagaaa agtacaaaag aaaagagaaa aatcaaaaag ctagaaagaa
     5461 aaagaggctg agacagaagc gtttaactcc aagaaataag ctggaattca caaaaatttg
     5521 aaaagcaatt ttcgtgaatt tcagcttatt tccgaaggag ttgcttctgc tttcgccgtt
     5581 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctctttaatt
     5641 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
     5701 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
     5761 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
     5821 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
     5881 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
     5941 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
     6001 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
     6061 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
     6121 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
     6181 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
     6241 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
     6301 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
     6361 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
     6421 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
     6481 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
     6541 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
     6601 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     6661 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     6721 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     6781 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     6841 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     6901 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     6961 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     7021 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     7081 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     7141 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     7201 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     7261 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     7321 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     7381 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     7441 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     7501 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     7561 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     7621 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     7681 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     7741 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     7801 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     7861 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     7921 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     7981 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     8041 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     8101 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     8161 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     8221 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     8281 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     8341 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     8401 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     8461 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     8521 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     8581 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     8641 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     8701 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     8761 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     8821 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     8881 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     8941 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     9001 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     9061 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     9121 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     9181 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     9241 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     9301 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     9361 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     9421 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     9481 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     9541 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     9601 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     9661 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     9721 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     9781 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     9841 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     9901 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     9961 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
    10021 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
    10081 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
    10141 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
    10201 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
    10261 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
    10321 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
    10381 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
    10441 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
    10501 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
    10561 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
    10621 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
    10681 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
    10741 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat gatggaagaa
    10801 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
    10861 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
    10921 tagacaaaa
//
LOCUS       DACDMM010000084        10623 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDMM010000084 DACDMM010000000
VERSION     DACDMM010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.085"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(592..1983)
                     /locus_tag="IWD16_001782"
     CDS             complement(592..1983)
                     /locus_tag="IWD16_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP9088799.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(2196..2582)
                     /locus_tag="IWD16_001783"
     CDS             complement(2196..2582)
                     /locus_tag="IWD16_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP9088800.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2638..3261)
                     /locus_tag="IWD16_001784"
     CDS             complement(2638..3261)
                     /locus_tag="IWD16_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9088801.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3325..3993)
                     /locus_tag="IWD16_001785"
     CDS             complement(3325..3993)
                     /locus_tag="IWD16_001785"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9088802.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(4005..4445)
                     /locus_tag="IWD16_001786"
     CDS             complement(4005..4445)
                     /locus_tag="IWD16_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088803.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4504..5176)
                     /locus_tag="IWD16_001787"
                     /pseudo
     CDS             complement(4504..5176)
                     /locus_tag="IWD16_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5361..5759)
                     /locus_tag="IWD16_001788"
     CDS             complement(5361..5759)
                     /locus_tag="IWD16_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP9088804.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5756..6529)
                     /locus_tag="IWD16_001789"
     CDS             complement(5756..6529)
                     /locus_tag="IWD16_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9088805.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6526..7209)
                     /locus_tag="IWD16_001790"
     CDS             complement(6526..7209)
                     /locus_tag="IWD16_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9088806.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(7376..8263)
                     /locus_tag="IWD16_001791"
     CDS             complement(7376..8263)
                     /locus_tag="IWD16_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9088807.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(8260..9129)
                     /locus_tag="IWD16_001792"
     CDS             complement(8260..9129)
                     /locus_tag="IWD16_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9088808.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(9266..10552)
                     /locus_tag="IWD16_001793"
     CDS             complement(9266..10552)
                     /locus_tag="IWD16_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9088809.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         3187 a         2305 c         1763 g         3368 t
ORIGIN      
        1 ctgttctaac attctcaact ttgtcagttt accataggaa aatgggaaaa tctacgaaag
       61 ctctcaacag aatcgtccta cttaaatacg cttttcttta ataataaata agttggttgt
      121 ttttgttatt tacgctttca cttttcggaa taaataatgt gtatttatat ggaataaata
      181 aaaaaagagg tcgtgactaa aaacaagtca caacctctgg taaacggtgt tctcctgaca
      241 actcctcggt cacaagacac aatattcaac aaacatgaga agctattttt tgaaattgct
      301 ctttgtgact cagagtgaaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag
      361 cttgcatctg cggtaataag ctcaaccaaa cgtaaaatat gaggaactat ttttgtttgg
      421 ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttattcac ggtgtttcag
      481 aagtagcttc tcggaaataa gccgaaattt cacaaaaatt tgagaaacta tttttaggaa
      541 acttcttctt atttcttaaa gctgaccact tctatcacaa cctctttttg actatgcatt
      601 cacatcgatc actttagaat catcattgat gacatcgcga tctaaatctt tgatgcgtgc
      661 acttgtcacg ataccagcta ccatgctacc gctgacgttt actgctgtac gtgccatatc
      721 gattaatggt tcaacagaga taactaaccc aacgattgct actggcaagt tcattgcccc
      781 taaaacgatc agtgaagcaa atgttgcgcc cccaccgact ccagctacac caaaagaact
      841 gattgttaca attgccacaa gcatcaaaat aaattccaaa ctaaagacat tcactccagc
      901 tgctggtgcg acgatcgttg ccaacatagc tggataaacg ccagcacagc cgttttgtcc
      961 aatagacaaa ccaaaacttg ctgagaaatt agctgttgca tcatctactc ctaaagcttt
     1021 tgtctgagtc tcgatattca atggcaaagc tccagcactt gaacgtgaag taaacgcaaa
     1081 gctcaatacc ggaaaggctt ttttgaaata gttgattgga ttgacttttg caccaatcaa
     1141 aatcagtgag tggacaatca aaacagcaag caatgctacg taagaagcaa cgacaaattt
     1201 acctaaattg acgatagctg caaaatcact tgttgccaaa acgtttgtca tcaaagcaaa
     1261 tactccataa ggtgtcaaac gcaaaacaag cgtcacgatc cgcataatga ttttatacag
     1321 actgtcgact aaatctgaaa agaatgctgc ttcttttggc gcttttcttt tcacgcctag
     1381 ataagcgatt ccgacaaatg ccgcgaaaat aacgactcca atcgtacttg ttgcacgact
     1441 acctgctaaa tcagcaaaaa cgtttttagg gataaaggat aaaatctgct gtggaatcga
     1501 taagttttca actgtttcct gacgtgttgc cagttcagag attcgtgctg tttctgcagc
     1561 tccttgtgtg aatttagctc cttggagtcc gaacagcatt gttgaaccaa ttccgatcaa
     1621 tgctgcaata gctgttgttc caagtaaaga agtcaataca gtcgcactaa ttttccctag
     1681 cttttcagaa actttcattt ttgtgaatgc accaatgatc gaaataaaca ctaaaggcat
     1741 aactagcatt tgcaagaatg atacatatcc ttgacctaca atcccgatcc attccattga
     1801 ttgagtaacg actttgtcgt ctacaccaaa gatcaattga agcagaccgc caaagacgat
     1861 accaactcct aatgctgtaa agacgcgtgt tgaaaatttt gtatggcgct tttgcatacg
     1921 atagaacaca tataataatg ccgcaaaaac tagaaccact aaaactgtga tgagtgttgt
     1981 catttttctc ctcctcatat tattaatgac aagtccatcg aaggaagtaa aggttcccga
     2041 gaatgattct tcaaccatcc tcctgaaaaa aattttttca gtgactttta tattttagac
     2101 gatttttatc gaaatgacat tctttttgct tacaattagt aataaaaaaa catacctgtg
     2161 atagctttaa tctatcgcag gtatgttttt actgtctatt cttgtttttc tgattgaaaa
     2221 ttcaatttta ccccaggctg atctaaggcg atctctgtca cctcataaga cattcggcgt
     2281 acgatatcga ataacggtgc tactagctcg tccacttctt cttgtgtgac atcattttga
     2341 ccttcaattt tccgattgag gatatgattg atctgactca cacttccaga taaaacatat
     2401 gtatcaaaca ctaaacggaa ctctaagcgt gctcctacga tgctatgttc tttcggatag
     2461 ttttcgatcg tttcttttaa aggcgagaag cctactttca aatctgtttc tacttcttgg
     2521 ttcggcaatc tcatatcata atgaaatgct tctacaactt ctttttgtct tctgatttcc
     2581 atataatttt atcactcctt tatgaaaatt ataacatatc tcctttgaaa ggggatacta
     2641 ctttttaatg atgctctgca aggtaaggat ttcattcaga tttcctacca cataatctgc
     2701 cgggatctca cccagaaaat gatcgatgtc gtaaagacaa gtagcgactc ctgcattttt
     2761 ccctgcttcg atatctaaac gacgatcgcc gatcatcatc gtgtctgttc tttctaaatg
     2821 aaaagtatcg atcaaataat ttaaagatgc tggatcaggt ttacgaggaa agtcctgatc
     2881 gatccctact acttcctcaa tcagatgtgc caatcgatgt tcttttaaca ggccccatgt
     2941 tgattctgtc aaacggtggg tcaggatgaa atgacgaccg ccgttgtctt ttagcatttc
     3001 cagtacttgt ttcgtttctg gaaatggacg ggaaacttcc gtactttctt tttcatatcg
     3061 gtgaaataat tcattaaact ctctggcatt caagtttgat tcattgatca aataactagt
     3121 agaatacttt ttcattttga agtagatttc ttttttgtcc atactgatac caaaatcttt
     3181 tagtgcttgc catgcaccat ctaccattgc gggatacgta tcaaacaatg taccatcaaa
     3241 gtcccatata taatttttca tgttcctttt gttccattcc tttgcttctt ttttctatct
     3301 ctcactttta gataaaggtg ttctttaaag tatttttttg tccagaattg tcaaatcatc
     3361 tgctaatgta tactggattg gttgtgcatt tgggatatcg atcgtatcca tttgatcttc
     3421 aggaacatct tctaaatatt ttgtcagtgc tcggatgcta ttaccatgac ccacaactag
     3481 taaatcttgc cgatctttta gttttggtgc tagatgatct tgccataagg gaatgatccg
     3541 attcactgtc atttccagac tttctccgta tgggatcaaa tgcggatcca agtgatcata
     3601 tctgcggtca aatctatttt ctgcggttaa cggcggaact tccgtatatc ctctgcgcca
     3661 tgtttttact tgatcttttc cgtatatttc tttcatctta tctttgtttt tacccacaag
     3721 atccccataa tgccgttcat ttagacgcca tgtttttatt ataggaagcc ataactgacc
     3781 catttcttct agtgcgactt gtccggtaag aatcgcacgt ttcaaaacag aggtataaac
     3841 ggcatcgaat tgaagtccgg cttcaagcat tttttgaccg gcaactgctg cttgttcttt
     3901 tcctttttct gttaaggcga catctagcca tcctgtccag taattttcaa aatttgcttc
     3961 gctttcgcca tgacgtaata atactaattt cattcttttc tcccttaaac attcgtttcg
     4021 aattttatac cacatgtttt gcatgtcaca cgtattcgtc cttttccttt aggtgcacgc
     4081 tgcttttgat gacaattcgg gcactcaaaa aaagtataac tttttcgatc cattttgact
     4141 tgctttttgt tttttatctg atgatatttt tctttgactc tatcaagata tttcaaataa
     4201 cgttgattct catttagtct ggggtagatt ttttttgata ggaaacgaaa tatcatccat
     4261 ccgaaaaaga cgatgaacaa caattgtccg attctatagg gcaaccaacg acttagcaaa
     4321 ctaaagacaa gcgcagcgat caatagaccg cgattcaact gatcaaattt cgcatatctt
     4381 ccctgcataa gtttgtgata gttctgctga aatcgtatta gcctttctaa ccatgtttgc
     4441 atcatattat ctcctccatc atctaatatt tattatgaca aaaaaatcca aaaagaaaaa
     4501 gccttaattg gccttttcca ttgcaacttg gtgaaggaca ttccgatttc tggtaaagtc
     4561 aatcacaatc actttctttt tatcttctct gttgactact cgaaatgtgt ccgtcaatgg
     4621 aagaaacagc ttcttagatg gtagctgttg catacgtata aaacgatgga tcatcgtaat
     4681 caattgagga aattgaatag acgtgtgaag cagacctgga tattttttca acagctgtgt
     4741 cgttttgatc agattccgtt tttttactga tcacttttaa aaagcttttt agaatctttt
     4801 gctgacgtct aaaaatagaa aaagaatttt cgtcatctat ttgataacgc atttgttttt
     4861 gcagttctgc tgcagtcaga tggatcttcc catcgtttgt atcgatcatt aaaccttctg
     4921 gaaacagttc aaccactgct cgatcccaag attcaagaga aagctcgata taatctttta
     4981 ctggtaattc caattctacc ttaaaaaagt gcaaaacagc cgacagccct tctgcttgat
     5041 agatttctcc taatgttagc ccctctagat ggaactctgg tgaaaaatgc agcaatgttt
     5101 tattttcctg caattgaaca tatagaaatg tagatcgctc aatcagagta tcttttaaaa
     5161 caactaaaaa attcattttt ctcccccttt tttcatcatt tttaaaatag catattttga
     5221 aaagaaagtc tgatgcttag agtagaattt cataaaaatt tactatttca taagtattca
     5281 cgagaaaaat acaaacaaaa aagcggaaat aacgctcatc cgccttcttg tttgtgttta
     5341 tttcctcttc aaacgcttgt ctatgattca gacttttctc tatcgttatt ttcatctaaa
     5401 agttcttgca ttccgataat ttctcgatat tgatgaatct tagtgatacc aatgactttg
     5461 tctacatcaa tactaaaaca aataccatta cctggaatat cgatgttgat gccttgacca
     5521 atcgcatcca ttactttatt cgtcaaacta tcgggtacta atgtcaacac aatatctttt
     5581 tctggcgaaa tgtccaaacc taaaaatttc cctgaatcat gtacaccaga acctcttcca
     5641 tgtaagatcg ttccgcctgc tgcacctgct tcttttgaaa agtcgataac atcttcgccc
     5701 attcctccgt caacgatcgt cacaatcatg tgaagattca atgctacaag atctttcatt
     5761 tgctttcccc caatttttgc aatctttctt tttcgattct gtctttttca tcttttcgtt
     5821 tataaatata tcctagtact aaagtggata atattggtgt caatgcaatc attgcaacca
     5881 caccaaaccc gtcaatgatc ggatctcttc cttcaatttg tgcagcgatt gccacgctca
     5941 ttgataaaat aaatgtcgaa cacatcgggc ccgttgctac tccaccgtta tcaaaagcca
     6001 ttgctaaaaa gatcttgtcc acttttcgtc ctaaaataaa tgctagcccg tatcctggga
     6061 tcaagaaata ataaagagag aatccgacga gcaaacgcca catagacaat cctactgctg
     6121 cgccgatccc aattgaaatt accatcagca tgattttcga tcggattctc ccctcgctca
     6181 attcttcaac ctgtttgatc attacatgaa tcgccggctc agcaaaccct actaagaacc
     6241 ccattaaaaa gccaagggga accagtatat aattgtaagg caatcccgca atagcactgc
     6301 ctaaatgctg tcctaccggt acataagcca tattgactcc gtgcaggaaa aggatcaacc
     6361 caatagtcgt tatcaagaaa cctttcataa tgtcaaagaa acgctttttg cgcatacgaa
     6421 aactaatgat attcattaaa acaaatagga ttacgatagg tgccactgcc aaaatgactt
     6481 ccatggtaac gatttcgatt ccatctatca cttgctgcca gaaactcatt ggaagatcac
     6541 ccccaaaatc atcacggcaa gaatcggccc taacgaagca ataccgacca ttccaaaact
     6601 gtcattttca tttttctttt gtttgatcat actagtcatc cctccggcaa gggccaagat
     6661 aaatggaaca gttactggac cagtagtcac tccccccgaa tcaaaagcaa tcggcataaa
     6721 ttcggaactg gtgaaaaatg aggcgatcaa aacgcctatg tagcctatag ccatcaattg
     6781 ataataggat agcttgaata cgacccgaag cagcgcaaag gctagaaata ctccagtgcc
     6841 gacgctgacg atcccaatca gcaacactct gccaatctct ccgtcagata tatcatatac
     6901 ttgttgtccc aaaacttgca cggaaggttc agcaatcgtg atcacgatcc cgatagcaaa
     6961 tcccaaactg accaaaacgg tcaaactttt ctttttgatc atatatttcc ctactagatc
     7021 tccgacttcc atcatagaat aatctgctcc aaacaaaaag agcgtcattc cgatcatcat
     7081 gattgccgca ccaaccagaa atgaagcgag gtcttctcct tctaacggtg caaaaacaaa
     7141 cgttaaaatg acaatcagga cagtcatcgg caatatagca gcaatcactt ctttaaaatt
     7201 ttttagcatg tatcctcctc ctttactctt taagtttacc ttaaaatgaa agagttttcg
     7261 agcacaaact taaaacgtta tcatttaatt tttctcaaca taaaataaca cacgtcccaa
     7321 aagtatcttt ttacttggaa aaatgacttt gaaggaacgc gcttattgtg tttttttatt
     7381 ttacagagcc gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat acgatggcag
     7441 ttggtaaagt agcgatgact aatccagcca tgatcattcc atagtctggt gtataggaag
     7501 cacccatagc agataaaaca agcggaaccg tccgtttatc aggagattgc aaagctacta
     7561 agggccataa ataattgttc cagctactca tgaatgtgat gattgcagcc gcggcatagg
     7621 tatttttagc cgtcggcata tacactctaa aaaaaatact tagttctttt agaccgtcta
     7681 atcgagcggc ttctactaga tcttttggaa aagatttaac attttgtcga aagaagaaga
     7741 tcaaaaaagc agtgctaaca gatggcagga tgacaaccaa gaaactgttg attcccaaaa
     7801 ccgttccgtt caattgtgaa aacattcgat ataaagggat catcaatgcc gcaaaaggaa
     7861 ccatcatcga tagcagtaga atattgaaaa ttaggtcttt tcgtttactt tgataaatct
     7921 caaatccata tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc accaaagcga
     7981 tcatcgctga attccaaagg gaacgagtaa aattcagctc attggaaaac aaattttgaa
     8041 aattgatgat caagttactg ccaatcttta tttttcccga tgtgatatcc acgggtgtat
     8101 tcgtcattcc caaaaccatc cagacaaaag ggaaaatgga aataaatgat aggatgctta
     8161 atgctgtata ctttaatact gacccaattg tcgaacgaag ttctaatgct tgttttttga
     8221 cgttggggcg ttcgattgtt tctttttcta ccatattttt catcgacttt ctcctcctac
     8281 tttcgtttgg attatcgaca atatcacaat aaaggaaaca ataacaaagg aaactgctgc
     8341 cgcatatcca aaattaggcg tgaatttata actcaaatta tagatgtatt gagatagggt
     8401 ggtcgttgca tttgctggtc ccccgccagt aatattttgt acttcatcaa aaagctgcaa
     8461 tgtcccgatc gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat ttgggatcgt
     8521 cactttgata aactgctgcc atttagaagc accgtcaata gatgctgctt cataaatctc
     8581 cgaatcaata ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag tccaacgcca
     8641 agtgatcgaa agaatgatca gaactctcgc ccaaattgga tgagtcaacc agccaatcgg
     8701 tgtgtcaatc ataccaatgc ccaacaacaa gttgtttaca aggccagatt gagaaaatag
     8761 acttttcatg atcagagaat aactgaccat cgaagtgata catggaagaa agatagcggt
     8821 tcgaaacaac cctttatatt tcaacttttt atcatttaaa aggttggaaa taaaaagtgc
     8881 tagaaacaac atgatcggta cttgtacaag taaatacagg aatgtattaa aaaaagcttt
     8941 tttaaaagtt gcatctgtca acatccgctg ataattacct aacccgttaa atgtcatatt
     9001 gacaggggga ccactttgaa aagacgtgat caaagccaaa accatcggaa taaaaaccat
     9061 gattgatatc aaaatgactg gcaagattaa aaaagcatgc ccgtttttga ataaatcttt
     9121 ctgcttcatt cttttccctc caattctatc attgattatc tacatgaaaa gacaagaaag
     9181 acaatatctg taaggcagac gtgatctttt ttgtcttttc gtttgtctga tgaaaaagcg
     9241 aagaaattca cttttttacc accgatcaat tagccaattg tgcttctact tgtttttgtg
     9301 tatctgctag taccttgtct gtctcttctc cattgatgat tcgatgaaga gcttcagcta
     9361 cgactgcttc aattgcatag gtctcatgtc cgtaattcac ttctggtatt tcagatgtcc
     9421 atgttgaaaa atcgtcaaag atcttttgac cactgtaaaa ttcagaagct tcttgataag
     9481 cttcttgttc attcgcgcta agcatcgtag atactagtcc gatttctttc gcaagagtgc
     9541 ccatcagttc ttcattcgaa gcaaacgttg cttctaggaa atctttcgct gaatcttctc
     9601 cagctactcc tttgatcaca taccagccag cgccgccaag gttagaagct tgcgcttttt
     9661 gcagattttc tggaagtgca ggtattggtg caatcttcca ttttccagat tggttttctg
     9721 ctccttggat cgttgaagaa taccaagcgc cagttgggct agaagctact gctcctgatt
     9781 gtatcgcagt tactccagca ttccagtcag aggtttgttc gactagatct tctttcaaaa
     9841 gagtggcaaa caattcaaga ccatacttta aagatgcatt gtcttggatc gtgactgtct
     9901 ccccatcttc agatgtatac cattctcctg cctgttgcat aatcatcctt actcgaccaa
     9961 ggtcagaagg attgacttca gcgattttct ttcccgtctt ttctttgaca tctcgcgcta
    10021 cttgaatata ctcgtcccac ttcagattct ccatatcctc ttctgtataa cctgcttctg
    10081 ccatcagatc cgtacgatag aagtttgctg ttacgcccga atcgaaagga acaccgtata
    10141 ttttatctcc tactttgttt accgcaaatt tatacggtgc aaaatgatct tcctttacaa
    10201 tagcagacaa attagcgaat gcatctggat aagaagtcaa atagccttgt atcctataat
    10261 cttcaatcaa gacgatattg ggcaatcctt ctgtattccc gctcgctaaa gcggtgttca
    10321 gtttctgaac gatatcatcc tgagacatcg taacaacttc tatctctgtt tcttcgttct
    10381 cataaacttt ttttgcttca ttcactgctt tgatattgaa tgtctcatcc cacgcccaga
    10441 cagtgacttt ttcttctttg tctgctgtac tactagcact tccgcatcct gttaagaaac
    10501 ctgatgctgc cgctaaacaa acgactccag caattaactt tctgatgttc atttttatcc
    10561 gctccttttc catccattag ttgtttacgt ttgctacatt catcatacca aaatttgaaa
    10621 acg
//
LOCUS       DACDMM010000085        10622 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDMM010000085 DACDMM010000000
VERSION     DACDMM010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.086"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(13..1275)
                     /locus_tag="IWD16_001794"
     CDS             complement(13..1275)
                     /locus_tag="IWD16_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9088810.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1268..2167)
                     /locus_tag="IWD16_001795"
     CDS             complement(1268..2167)
                     /locus_tag="IWD16_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9088811.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2312..4090)
                     /gene="uvrC"
                     /locus_tag="IWD16_001796"
     CDS             complement(2312..4090)
                     /gene="uvrC"
                     /locus_tag="IWD16_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP9088812.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4272..4586)
                     /gene="trxA"
                     /locus_tag="IWD16_001797"
     CDS             complement(4272..4586)
                     /gene="trxA"
                     /locus_tag="IWD16_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP9088813.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4673..7033)
                     /locus_tag="IWD16_001798"
     CDS             complement(4673..7033)
                     /locus_tag="IWD16_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9088814.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7148..7695)
                     /locus_tag="IWD16_001799"
                     /pseudo
     CDS             complement(7148..7695)
                     /locus_tag="IWD16_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            complement(7753..8190)
                     /gene="zapA"
                     /locus_tag="IWD16_001800"
     CDS             complement(7753..8190)
                     /gene="zapA"
                     /locus_tag="IWD16_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP9088815.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8392..9309
                     /locus_tag="IWD16_001801"
     CDS             8392..9309
                     /locus_tag="IWD16_001801"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP9088816.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9565..10476)
                     /locus_tag="IWD16_001802"
     CDS             complement(9565..10476)
                     /locus_tag="IWD16_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9088817.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
BASE COUNT         2989 a         2274 c         1792 g         3567 t
ORIGIN      
        1 tttttctata ttttatgctt tttttcgttc atttctcata attgaaatcg attgtttcaa
       61 tgaactccat aatccgcctt cgctatacac gagtacattc gatttgtaca tgtttgcaga
      121 caagatcatc aataagaatg ttacggctac aagtaccaac aaggaaatca atgctccacc
      181 cataccgact gtctcatttg ctaagcgaac cggcataata tatgaagata agaatggaat
      241 atacgaagtg atccgaataa cgatattgtt tggatctgaa gctcctagta tcaatccaag
      301 catatatccg cctagagata gataagtcac tggcaaaatt gcttttgctg tatcttctgc
      361 tttgttgacc aaagagccac ataaagctgc taaaacagaa aaaatcaaaa tgccaaagat
      421 catgaataga agagaatacc acaagaatgg tccaatcaag ttatccaatg agataccgtc
      481 taatatattt ttgaccattt ccatatcttt gaattgactg tatcctatac caaaaataat
      541 accgtataat gccatttgcg ttaatgcaac aagtaaaaca cctgttagtt ttccgtaaaa
      601 atgcttttgt gcagtcgtac tggacaggat cacttccatg atccgcgtgc ctttttcaga
      661 agcgatctct tgtgcaatga tctgcgcata cgtcaaaata ataatgaaca agacaatcgt
      721 tgctacataa cttacgacat attggactgt cgagttatct tctccaactg tcatttttcc
      781 ttttgaatca aaattgactt tttgtcgagt caaacttgct tgttggctca agctagctac
      841 ttcttctggt gaaatgccta attgacttgc tcgaagcatc gattgcaagc cagtcagttg
      901 ttgttgtatc agtagttgtg tactttgtcc taatgaattt tcgctataaa gcatcccatt
      961 tatcttatca ttctcagttg agactactaa atacgcatcg attttttctt ccgaaagctc
     1021 tttttgtgct ttttcttcag aatcaactgc tttgaactca aaatcatctg ttttgacttg
     1081 gctcatttgc tgactgattt gctcattatc agccaatacg ccgattttat tgacatcact
     1141 attttgttga gcaaagtttc ctgctacata aatgataccc agcatgataa aagggactaa
     1201 tatcatgatc aagaaagaaa tcgacttaac attttttaaa tagacatctt tggtaataat
     1261 ccaaaactta cgcatcgatt tcacctgctt tcaatttgaa aatctcttcc aaagttggcg
     1321 gttgctggtt aaacatagga atgtagccga actgagtcgc acgagtaaag atttctttcc
     1381 cagcttcagg atcttctaag gtaatctcta acgtattgtc ttcatgaagg atcacttttt
     1441 tcacgccttc gatttcttct acttcctgtt ggcttaaacc agattccaag aataacttcg
     1501 tacggccaaa tgattcacga atctcatgta cttttccatt caaaaccatt tttccgtttc
     1561 gcaacatgat caaatgatcg cagatttttt ctacattatc catattgtgg ctagaaaaaa
     1621 tgacacatga accatgtttt ttcaattcca atattccatc ttttagcaat tcagcattta
     1681 ccggatctaa cccgctgaat ggctcatcca aaatgatcaa tttaggttca tggattaacg
     1741 tggcgatcag ctggactttt tgctgatttc ctttagacaa agattttact ttatccgtct
     1801 ttttcccttt tacctgaaat ttttccatcc aaaaatcgat ttttggttca atctcttttc
     1861 ttgatttccc tctaagttct gcaaaataaa gcagttggtc ttgaatcgtt actttcggat
     1921 aaagtccccg ttcctccgga agataaccga tgacatcata atctttctcc ccaagatgat
     1981 ggccattcca taacacttct ccttggtctt gtgtcaaaaa atccaagatc aaacgaaaag
     2041 tcgttgtctt cccagccccg ttttggccaa ttagccccat gatttttcca tcggggattt
     2101 ggaaagagac atgatcgaca gctgtgtagt tcccaaaagt tttaacaaga tccttgactt
     2161 ctaacatttc ttcactcctt cttcaagaaa tcacatatat ccttagacac cagacgtaag
     2221 cgatttatta tttaccaaca aaacgtaaac gctctttttg ttgtacataa aatgatagga
     2281 cactttactt tctttgtaaa gtgccctttc ctcattcctg atgtaattta tcaaatacgt
     2341 tttgtgcgac atttttcggc agtccgattt cttgtaattc ttctgtcgtg gctgctgtga
     2401 tatttttcaa cgatttgaac tctttaagca gcattttttt tcgttttggt cctaaccttt
     2461 caatttcatc taatcgggaa gcaaaactgt ttttactgcg tagctgccga tggaaagtga
     2521 ttgcgaatcg atggacttca tcttggattc gctggagtaa gaaaaactct ggtgaatttc
     2581 gctcaagcgg tactacagcc aaatctatac cgaatagcaa ctcatttgtt ttatgtttat
     2641 cgttttttgc catgccagca ataggaatat cgattcctaa ttgattttct aagacatctt
     2701 ttgccacatc tacttgtcct tttcctccat cgatcaagat caaatcaggt aaaggcaacc
     2761 cttcgcgaat cactcgtgaa tatctgcgat agatgacttc tctcattgat gcataatcat
     2821 ccggtccgtt gactgttttt attttatatt tccgatattc ttttcttgcc ggttttccat
     2881 caatgaatac gaccatcgca gccacaggat tcgtccccat gatattagag ttatcaaaag
     2941 actcgatacg tattggggtc gggatattca tcgcgtttcc taaacgttca actgctccga
     3001 tcgtccgttc ctgttttctg gcaatcagat caaaccgttc attcaaagca actgaggcat
     3061 tcttattcgc caattcaact aattttttct tttctcctcg ttttggttgc agcactttcg
     3121 tagctaacaa agcttctaca ctgggcttgt cgatcgtatc aggaatcaag acttctttag
     3181 gaatgaaatg ctcattttcc tgataaaact gaccgaggta agtcaagaaa tcttcttctg
     3241 cttcgttata gaaaggaaac attgaaacat ctcgttcaat tagctttcct tgtcttacga
     3301 aaaacacttg gacacacatc catcctttgt caatcgcata accaaataca tctcgatcca
     3361 gtaaatcagt atttgtcatt ttttggcgag tcatgattgt ctcaatggca cggatctggt
     3421 ctcggtattc tgcagctttt tcaaactcca tgttttcagc agctttttcc attttctgat
     3481 ggatctctga ctcgattttt tcgtgtcctc cgtttaaaaa ctttttcact tcatcaacga
     3541 ttttcgtata gactttcgga tctacgtcga aataataagg acacagacat tgtcccaaat
     3601 gatagtaaag acaaggctcc ttcgtctgac taggtttgca ttttctcaga ggaaataatc
     3661 gatcgaggat cttctttgtc tcattcgccg caccgacatc gggatatggt ccaaaataca
     3721 gtgctttgtc ttttagtact ttacgagtaa tcaccaaacg gggatatttt tcattcgtta
     3781 tcttaaggaa aggataggtt ttatcatcct ttaacataat gttgtattta ggatcatttt
     3841 tctttatcag attgatctct aagagcaatg cttcgatatt cgattctgta acgatgtatt
     3901 caaagtcttc gatttcacta acaagccgtt ccgttttcgt attatggctg ccagtaaaat
     3961 atgagcggac acgatttttt aatattttgg ctttgccgac ataaatgatc gttccatttt
     4021 tatctttcat caagtaacag ccgggctgat caggaagtaa agctaatttg tttttgattc
     4081 gttcgttcat ctggtactcc ccttcatatt caattaaagt tattatagca tgtctgtcaa
     4141 aaaaacaatc cagcctgaca acgtaattat actcaaaaaa tattaatttg aataacctta
     4201 actcaaccgc aaaaaaaccg aggacggaac tggattcagt cccaccctca gcttattttt
     4261 gctgtaagtt tttataaata ttgatcgatc atttgacgta gttgttcttt tgaatgaaca
     4321 ccaacagctt tttctactac ttgaccatct tttttcaaca ttaatgttgg aatgctcatg
     4381 ataccgaatt gttgaggagt ttctggattt tcatccacat ccattttagc aattctgaat
     4441 tcttcttcat catattcttg ttccaactga tccaaaatcg gtgcttgcat acgacatgga
     4501 ccacaccaag ttgcccaaaa atcaatcaat acaagtcctt tatctgtttc ttcattaaat
     4561 gtcttatctg taattacttg tgacatatca aagacctcca tttttaaaat ctatgattag
     4621 tatagcattt ctatacttgg atagcgaacc atctgcttac ctttagtaag ctttacttaa
     4681 atttaacaat tgtcgcacca tttccgcctt ggtttgccgg tgcaaactca aagcttttga
     4741 cgctccgatg gtttttaaga taatcagtaa ttcccttgcg aagtgcgcct gttccttttc
     4801 cgtggacaat agtcacttga ggataaccag ctagaatggc agaatccaaa tattgatcta
     4861 cctcacttag cgcttcttcg tatcgttttc ctcgaaggtc caattgattc ggcacgtgac
     4921 tactagattc agatcgtaca gcatggataa ctcgttgtgt cggctctttt tgaggagcaa
     4981 caggcgtcag ctcgtcttcg gacacattca tttttaatat accgatttct acttgccact
     5041 gattcccgtt tttacgtaaa agcgtccctc tttggccgta agtcgtaacc aacacttcgt
     5101 ctcccggttt caacgttttt tgttctttcg ccttcttcaa tactttattt ttcttcaaag
     5161 tttcttcttg atgtagattt gctaattggg tctttgcatc gatcaactga tgctctttca
     5221 catttccttg cccaatctgc tgttgcattt tacgaatatc agcaatgatt ttttctgctt
     5281 tttcttccgc ctctgacacg acttcgtttg cttttttctt tgctttttcc atttcttttt
     5341 cacgttcttc aaagaaatag ctgtaagctt ctttcaaatc atcgtgaagt tcttgtgcct
     5401 cagaaacaaa atgacgcatt tccagatatt cggtctctgc cattttccgg cgattctcta
     5461 aatctgtgat catttcgttt aaatcttggc tctcatcatt catcaactgc ttcgcttcat
     5521 caataacttc tgtatcaaga cctaatcggc tagagatttc aaacgcattg cttcgtccag
     5581 gaacgccaat cagcaaacga tacgttggac ttagtgtatc cacatcgaac tccatgctgg
     5641 cattaatcgt atttgcccga ttgtatccat aaactttcaa ttcaggataa tgcgttgttg
     5701 ccatcacata agcagacttt ttaccaagat catctaagat tgcgattgct agagcagcac
     5761 cttcttgcgg atctgttcct gcaccgagct cgtcaaataa taccagactt ttttcattga
     5821 cttttgataa aatatctaca gtgttcgtca tatgagaaga aaaggtcgat agactttgct
     5881 caatcgactg ttcgtctcct atatccgcaa atacttcctc aaaaataccg atttgacttt
     5941 cttcacctgc aggaatcggt aatcctgctt gccccatcag ttgtaacaga cctaaagtct
     6001 tcaacgtgat cgttttccca cctgtgttag gtcctgtaat gacgatggct tgatagtctt
     6061 ttccgattgt gatgtcatta gaaactactt tttcctgatc gatcagtgga tgtcttgctt
     6121 gtttcaagac gacatgattg tcttcactga ttcccggcac gatggctttt acttctttac
     6181 caaatcgtgc ttttgcattc atcagatcca ttttaccaat cacataagca ttgtggatga
     6241 tttcttgacg atggggaaca agttcagcag aaagttcact taaaatacgt tgaatctcat
     6301 ttcgttcagc gatttggtat tgacgcaaac gattattcaa atccaccact tgttttggtt
     6361 caataaataa agtctgtcct gaagcacttt gatcgtggac tacaccgcca aatacaccgc
     6421 ggtattcttg tttgactggg ataacgtagc ggtcatttcg catggtgatg atcgcatcac
     6481 ttaaatactt tgcatttttc ccacggacga tcccatctaa ctgttcacga acagcttgtt
     6541 cacttcgccg aatgttctgt cttatgctct ttaactcagg agaagcgtca tcagtcacgc
     6601 gtccgtcttc atcgattgct tcctttaaac ggcggctcaa aacagggatc gtgactaact
     6661 gatctgccca catatacaaa cgtgcaaatt cgatttcact gtcagaaaga tcatcaatga
     6721 aacgattcaa ttcgcttgtc gtagacaata ctctggaaac ttgtgcaagc tccactccat
     6781 tcaagtctgc tccgatctcg atccgtttca tatggggacg gatattttca agtttaggta
     6841 tagggatccc gcctcgcaga cgttgcactt tcaatccatc ctcagtttct tgaagccagt
     6901 ttgtgatcgt ttgtttctca ctcactggta ccaattctgc cagttcttct tttccttgtg
     6961 cagtcactac aaattgtctg accatctgtt tgactttttc aaattccaat gtttcaagga
     7021 ttcggttatt cattttatca ccttatcttt cactatgaaa aaagagctgt gccggacata
     7081 cgctacgcag ccttcttaaa taaactactc tgtacaaaaa acgaaatatt ttccagcact
     7141 attttcctta attcaaaata ttcgttaccc aaagctcata catcttgttg gtaagaaatg
     7201 gcgttctctc aacgataaaa cgagcgaaac tatgctcgcc gaattgattt tggaccacac
     7261 taatggaaca aaggcaagta actgcaaaat caaaaatata gtaacgtaag ccatgattag
     7321 tgacaggatg cctcctgcaa tccagtctac ctgtctcaag atcggcacat acgtcaatcc
     7381 atggacaaaa acccccacaa atcgggtaag cagccagcca attagcaaaa taatcagaaa
     7441 agcagttcct gcataaaaag cttcatctag gtgaaaagca aatacctgat caaaaaatgc
     7501 taactttgat gttggggtga cagctgggta tggaatgtat aattccaaat gacttgctaa
     7561 tggtttgtaa aaatgctggg cagcaataaa tgacaataca tatccaatcg cgaatatgat
     7621 ttgcaaagca aacccgcgac gtgctccaga aaaaaacgca attagcaata aaaataaaat
     7681 cagcaaactc agcatgctac gttcctttct cttctaactt ttttaaattt ttcttatttt
     7741 gataaaagga tcttaattag aagatttttg tttaatttgt ccttttcgtt tctcgtttaa
     7801 aatctgttgt gcttccacat gatttttaat ggtatgatct gcttgaccat tttctttcaa
     7861 tacttcccga gcttcgttct cgattgcttc aatgcgtttc acacgatttt caagctctgt
     7921 aaatttgatc atttttttct ttaattctgc tgtttcttct tccagctcca agattctttc
     7981 ttgtttcttc aattgatccg aaagcgcatt gacagccatc aagattgctg cttgttcatt
     8041 atccatttgt ggtgataatt gtttcaattc tgctaactgt tcattgatca gcttcgttac
     8101 gatatccata tgatgcttgg tttcccgtcc gataatagta tatgtttggt tggcaatcac
     8161 tgctttgtag cgtgtttttt cgtgcgccat acgaatcatt tcctcctatt tgatgaaagt
     8221 tttatttagt gtaccatgaa gttggttctt tcaccgttct ccacaaatat ttttcacata
     8281 cttaactatt atatgataaa ctttaaaaaa atgctatgtc tcgcaatctc ttagaaaacg
     8341 aaaagtcgtg agttgcggta tagttttatg tataaaaacg aggagaattt tatgagcaac
     8401 gtcattatca aagcaacaaa cgaccaactt agtcaaatga aagcatacta tcgctcttat
     8461 ttgttaaata aacagattcc ttatactagc tttgctgcaa aaaaagacgg gacgaccatt
     8521 accgcttata catccggaaa agtgatgttt caaggtacga atgcagcaat agaagctgat
     8581 aaatgggggg aggcacagga taaacaagcc aagccagcaa aaacagcctc ttctttgcca
     8641 aaaaatatca gtcaattatc cgttttaggt agtgatgagg tcggaaatgg cagctatttt
     8701 ggtccgctaa cagtatgtgc tgcttatgtg aaacgtgatc agcttgcaga attacgtaaa
     8761 cttggtgtta gagattcaaa agagttaaaa gatagccaaa tcatccagct tgctgacatc
     8821 ttaaaaaaaa ccatccccta taaactgctt gtcttatctc cagaaaaata caatgagatc
     8881 caaccaaaat acaatgccgt tcggatgaaa gtcgctttgc acaatcaagc gatccatttg
     8941 ctgctaaaaa aaatcgagcc agaaaaacca gaagcgattt tgatcgatca gtttacgccg
     9001 gaagcaaatt ttaaaaaata tgcgcgttta gaaaaaaatc aattacaaga aaaactgtac
     9061 ttcgttacta aaggagaaca atatcattta gctgttgctg ctgcttctat catcagtcgt
     9121 gccagctttt tggaagagtt acacaaagca tcagctgaag ctggaatcac ccttccatca
     9181 ggagctggaa cgaaatctga ccaagtagcg gcaaaactat tggagaaagg cggcatgcct
     9241 atgcttgaaa aatatgccaa acttcatttt gccaatacag aaaaagcttt gaagctgcta
     9301 aaaaaataat ggaactgtga caaaagtcat gtcacagtcc cttgttccga ataaacggtg
     9361 ttctcatgcc aactcctcgg tcacaagtca caatagtcat caaacatgag aagctgtttt
     9421 ttgaaattgc tccttgtgac gcggagttaa acggcaggct cacaacctct tccttaaacg
     9481 gtgttcaaaa gcttgcatct gcgataataa gcccggccaa acgaaaaata tgaggagcta
     9541 tttttgtttg gctgggctta tttattactg aaaataagtg gtcaatgctt gtgtcaaact
     9601 tgcagcaatc tgctgctgat aactgtctgt tactaattcc tttaaatcac tatcattatt
     9661 catataaccc atctcgatta aaatagaagg ttgaagattt tctcgaagaa cttgatagtt
     9721 cccagtggag attcctcttg aatccagagg cagatcagtg aaggagtcag caattgtctg
     9781 tgctaatgtt tcctcttgtc catagtaata atatgtcgtg attccagttg cttcattcgc
     9841 atattccgta gagtctgcgt gtaaactgat aaagacatct gccttttttt cttcactgta
     9901 gtcgcatata tctccaagct gtactaattc atctgtagta tgggtcaaat aaaccgttgc
     9961 tccagcatcc tctagcgcct tttttacttt tatggctgta cttaatgtta tatctttttc
    10021 ttctgtttgt tcatcattgc ttaatgcccc agtatcattt ccgccatgcc caggatctaa
    10081 tacaatcact gcatcagata gctcttccgg ttttgtttgt atattctttg tgactttggt
    10141 tccatcttcg agggctaaat atccagtata gccatcattt gtcttgatct gataagcatt
    10201 ttttgtttca gaaagaaact ccgccagctc tccgccgttt atagttgcaa gtggggtagt
    10261 tagcgagtca tctgtatata gtgtacggtc attttttccg attttgatta atgtctcttg
    10321 ttctgtatca tcaaatgatt cctttgtctt tgtaaccgta ctgttcgtac tgctgaccgt
    10381 ttgtccaact atcttttttt gaccaaaaac atctatttga aaacagataa taatgataat
    10441 gattgcaaat acaaaagctc caattatttt tttcattctt gactctcacc tcgtagatcc
    10501 agactaaggg tcaaaactta aaatcagctt aagtacgcaa aaaaggggct gtaaaagata
    10561 taaccgtaga aaataacatt actgctaaaa tgattttttt catttttggc atatcctgaa
    10621 at
//
LOCUS       DACDMM010000086        10423 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDMM010000086 DACDMM010000000
VERSION     DACDMM010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.087"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IWD16_001803"
     CDS             complement(25..144)
                     /locus_tag="IWD16_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9088818.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IWD16_001804"
     CDS             complement(159..1343)
                     /locus_tag="IWD16_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9088819.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="IWD16_001805"
     CDS             complement(1599..1853)
                     /locus_tag="IWD16_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088820.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="IWD16_001806"
     CDS             complement(1878..3020)
                     /locus_tag="IWD16_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP9088821.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="IWD16_001807"
     CDS             complement(3080..4432)
                     /locus_tag="IWD16_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9088822.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="IWD16_001808"
     CDS             complement(4503..4847)
                     /locus_tag="IWD16_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9088823.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="IWD16_001809"
     CDS             complement(4857..5231)
                     /locus_tag="IWD16_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9088824.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="IWD16_001810"
     CDS             complement(5244..5558)
                     /locus_tag="IWD16_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9088825.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IWD16_001811"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IWD16_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="IWD16_001812"
     CDS             complement(8976..9638)
                     /locus_tag="IWD16_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088826.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
BASE COUNT         2851 a         2332 c         1768 g         3472 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aag
//
LOCUS       DACDMM010000087        10413 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDMM010000087 DACDMM010000000
VERSION     DACDMM010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.088"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(124..1488)
                     /locus_tag="IWD16_001813"
     CDS             complement(124..1488)
                     /locus_tag="IWD16_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9088827.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1485..3044)
                     /locus_tag="IWD16_001814"
     CDS             complement(1485..3044)
                     /locus_tag="IWD16_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9088828.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(3100..5580)
                     /locus_tag="IWD16_001815"
     CDS             complement(3100..5580)
                     /locus_tag="IWD16_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088829.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5564..5899)
                     /locus_tag="IWD16_001816"
     CDS             complement(5564..5899)
                     /locus_tag="IWD16_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088830.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5913..6947)
                     /locus_tag="IWD16_001817"
     CDS             complement(5913..6947)
                     /locus_tag="IWD16_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9088831.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(7037..7717)
                     /locus_tag="IWD16_001818"
     CDS             complement(7037..7717)
                     /locus_tag="IWD16_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9088832.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(8001..9104)
                     /locus_tag="IWD16_001819"
     CDS             complement(8001..9104)
                     /locus_tag="IWD16_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9088833.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9733..10203)
                     /locus_tag="IWD16_001820"
     CDS             complement(9733..10203)
                     /locus_tag="IWD16_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP9088834.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
BASE COUNT         2976 a         2134 c         1818 g         3485 t
ORIGIN      
        1 ttgataaatt tttggcgaat attatgctca aaaaattctt ccgctatttc attatatgaa
       61 ttttttattc tcgattgatt aatatttatt atatccccac acaataaaag accgtgatat
      121 ttctcacagt cttttatcta ctctattgct atttgctctc ttagctagta ctttaaaagc
      181 taactcattg aggtagtgaa aacgagaggt gtgttcagac agaaatccaa aatcctcaac
      241 tgctagctgt ggaatcgtag ttttgtgttt cacgatatcc gcaagcaggt tcaaatctga
      301 taataagaat tcttggctta tcagctgtcc gccaattatt tctaatgtat ccggatggta
      361 gatcagcttt gcatttactg ttttgttttc ttcatcgtat tgtggaaata gatcaaattc
      421 tcctgaacac gaaccggtat tttgttccaa gaatggcgct tcatcttctg tcagccctac
      481 agttcctaaa aagtagtcta taatgactgc tgctgttatc ttttgtctga ggtcgtaagc
      541 ggttggctga ccactcaaag ttagcgctac tgctcttgcc atatgtacag ctcgagtaac
      601 taaaggtaaa aagagatgcc gtttattttt gtcaaaactg actgggacga tatctccaat
      661 cgcatagatg tccggatcac ttgtctgcag atattcattg actatcacag tctcatcctc
      721 atacaaagcc actttttctt ttaacaatgt cgtattagga cgagtttggg cagaaaaaat
      781 gaccatatct gcctttattg cttgagatag aagttgtacg gatactactt tctcttctga
      841 ttttttccaa tcgatcaaga attcatttgg ataaaaattg atttgcttag tttgcagctg
      901 attttgaaaa ctgttgatca tatccttatc gaaatatctt gataaaacat tcggcatata
      961 atcaactaga tgaataatgt atcctttatt tctcaaagca tctgctaatt ccaatccaat
     1021 ataaccagca ccgaagatca ctacttcttt tgcttctttt aaagcggcaa gtacttgctt
     1081 agattgcact agatttttat aggtgtagac attttcagct tcaatttgtt tatacaaggt
     1141 gacaggagag gaacctgttg ctacgattaa tttatcatag gttatggtct cgttttttcc
     1201 ttctttggat gcgaggatga gctgatgttt gtctggatca agttcagtta ctctccattc
     1261 ggtgatcagt tttgccccac tttcttgaag agtccgcatg ttatgggcaa cttcagctaa
     1321 actacgtatc tttcctttta aataaaggtt gattccattt gaaacatagc ctagtccatc
     1381 tttatgatct tcatcgatca gtaaaacttc tgctttcggt tgaagttttc ttagatttag
     1441 ggcagctgtg atgcctgcat gggaagcacc aatgatcacg attttcatgt tttattcatc
     1501 cttaaattca atttttctaa ataacattct tttaaaaatt ggttcaaata ggggaaatca
     1561 taattatatt cgttgctagt gaatacaaaa actttagcgg acttctgtga cgggtactct
     1621 ttcaattggc tagtcaaaat gatatcatac tgcttttctt tctcgtattc gactaaatca
     1681 tatttgatcg tattcagcaa ttgttcctgg atgatttctt tcacgatatc cgccattagc
     1741 ctttcataag gaagatcata agcaatttgc agacgtaatt ctaactgctt gatcagcgtc
     1801 cgcaaaatag atgcatagcg agcaaataat tcaaagcggg cattttcctc gtctacttca
     1861 gtaaaatcaa caatagcaaa tgtttgtctg accaatcttt cacataaaac caggaattgt
     1921 ttatcttcta cttcagttaa ccgctgtttt aatccttctt ggccaaaaat attcatccat
     1981 cctctaaaat aggcaaaatg agcgtgattt tgcatcactg tcaacgcaat cttttgcgaa
     2041 atctcttctg gaaaaacata aaaagagaaa tattcttcta tcgattgttt aaacgttcga
     2101 ttcaattgag ctactacgtc gtctttgtat ttttcgctta tcaaaaactc ctggacatgc
     2161 gagttatgtg tatctaatgt aaagttactg atcatgaaaa gataaaaaac caacgtttca
     2221 ttttgattcc aaggaaaggc atattgaaag aagtaacggg tcaaaaatgt atggaggtcc
     2281 aataaaaatt gtgtcgtttg aaacaatcca acaggaagat tgctataatc tggtttggat
     2341 tttttcgtac agcgcattcg cttcttcata atggaaagcc atatttcaaa tttcaatcgt
     2401 ccttcttctg taatagtaaa actaaactct ttttgcagca gatctaaaaa agtgtttcct
     2461 gataaatcac tgtattcttt tttgttttca agataaggac gtccaaacca aaataattgg
     2521 aaataaaaat aacaaatttg actttctgga ccggtcagcc gcccgctttt tattttcaga
     2581 tgaaactcat ttaaagaatc atttaattcc ttcactctgc gagacaaagt agccgaactg
     2641 atattaagtt ccatgacaag tttctgccga tcaaatgagc cattttccaa cagataaaga
     2701 agtatctgat attttgtaga atagcgcaag tagcaatgat aataaacatc gactgctgct
     2761 atatttccat gtctgattaa taaaatattc ccttcttgga taacaatttc caattgtttg
     2821 atattttcca agttagcttg taaataaaac agatcttctt tcaaggtagc ccgatcaact
     2881 gataagcggg taagtacgtt attaacggaa atctctcctt tttcctctaa taaaagttgg
     2941 aaaagtttga tttgccgttg ttcatttttt tcaagcaaac tgaaaagttc caagtccact
     3001 cctccaagca tttctgattt taacagcaga agtttccttc tcatccttga aaaagaggat
     3061 ctgtgacagt tatcacagat ccatacaaat tctaatcact tagctggttg gcccagctgt
     3121 cagttcccat gtgaccatgc cttgatacgt tgcgccaggt tgagcattcg tactgctatt
     3181 tacctctaag agtaaaccat cggtatcggg aaagtcaacg agaacctctc ctttagctgt
     3241 tccatcactc tcataaatca acgtgttttg accttgctga atcaattgtt ttgatccaga
     3301 atgattgaag tataattttg ttccgcttaa ttcttgacct tgatcattct taaataaaga
     3361 agtcaatgtc cctgtcagtt gccaaggaga cgtgcttgga agccgagtat cccgcacatc
     3421 aaaagaaaaa tcgccttttg cttcataata acttgtcttc aacggtttgg ggagatcgtt
     3481 accaaatgta aaatcatctg gtacattaac taatgttaat tcttgtttgg ggacaagaat
     3541 tgttttagca gatgtttgga caactgggtt aggaagctta gcaccatctg ctaatgtaat
     3601 attgctggaa agtacagctg cctttgtcag tatgctgacg gattgtacag aagtatctac
     3661 agtatttaat tgatagttgt aagtaagatg aatggtatct cctattttgc tcaatagatc
     3721 tgctgggaca gaaactcctt gttgaatgcc ggtatctgcg atagttgtac catttaattt
     3781 taaggaacca ttgatataag tagtattttc aggaagtgag atcgtgattt ttttgtcggt
     3841 tatcgcatcc ccagattttg ttaacgtgaa tgtcacattt tcttgaatcg tatctcctga
     3901 tagagcagac aggggatctc ctgtctcagg ttgtctgcta aggtttttta gctgactatc
     3961 tgcactcaca gatggaaaat tagccactgt tatttgagga agagtggtga ttccagcttg
     4021 ttcagactgc catttgtacg caaaagacgg atacatgtta gtaagtacat cgtccaccaa
     4081 gcgataatca aaataatatt gccctgaagc caatgcaggt agtgtagaag tagctgtatt
     4141 tacacccccg ttatttgatt ggatcgtttg gaaaagttga tctgtccatc ctgtcgctaa
     4201 tttttgggtt cctttcgaat aaagcctgta atggatcgaa ccctttttta gttcagttgc
     4261 tgaccaattg gccgtgactt ggtacgatgc agaagaaagg agctttcccc actgactatc
     4321 ggctgttaag tttgttggta aaaccgttcc gtctagcttg tactgctcca atacctttaa
     4381 agcaacagga gaaagcaatc cgccttgcgc atctttccca ctgtacacga tattggctgt
     4441 ctgattcaat ccacttgtgc ttgctggagt atgagtcagt attgctcctt gtgtaacgtt
     4501 ttttcctgta aaatagttga ctacgcgatc aaagcgacta aatggcaact gatcaaaacg
     4561 tccttcagtc agccccatcg tcaatgtccg actattggta gagcctggaa ggttctggtt
     4621 atttccatcc acgtattctg tggtatcagc aaatgtatta tattttcctg ttgaatcact
     4681 tgttgagatg taactattcc acgtatttat ctctgcactt gcagaaaatg catatgctcc
     4741 tggattcaag tttccgccag ttaatttagg ataaaatcta cctgcagctg gagctaaaat
     4801 cgaattgaac atacgatctt ggataatatt attcgaatca taaaaatcaa cagaaaaaac
     4861 ggccttttta gacatagaag gttgtaattt acccttggtc aaaattttgt ttggactcgt
     4921 atcatattta tctccgtttt ctgtcatctt cgcgacatta gctgtaacat caaattgaag
     4981 cgggatatcg tccacgttga attcgcctcg cgtaataata gcaataatat ctgccaatag
     5041 agtatttgta tcccacgacc ccatcccaaa acgcaaggta tggcttcctg ctgataataa
     5101 actaccgcta gtaaccttga tgggggcacc ggcaatatac aagtaggcgt tgttccagtc
     5161 aattgctttt aatacatcac tgcttgatac atgttccggc aaattgatga tcgccacaaa
     5221 tgcaaatcct tgtgcagtga tattcaccaa atcaagtaca tttgttagtt tcacatggct
     5281 ttcccaatga aaacggaccg tgccaccttt ccaaggatca tttgaaccat cgttcaaata
     5341 agtagcagtc aatttaggca attggttagc cccagttagt tggacaagct tcccgccatt
     5401 gactgcacta cgtccaccat ttggaggtac gatattttca tacgtgtatg gatcataaga
     5461 aatggcagga ggcgttgctc caacagcttc tgccgaaggt gaaaagataa atatacttaa
     5521 aacaaaaagc gaagtaaaaa agaaggagcg aaaaaatgaa aatttatttt gtagcaacat
     5581 gttcatcctc cttttccttt cttctccata acaacaacca cgcaagaagc cataattcta
     5641 taccaatgat caaataaaga aaacggatct tttcttcatt cgtctccggg aatacgccat
     5701 gttgattaga ttctttataa atcggtagct gtgtctgctg ttttgatgcg ccattgactg
     5761 aaccacttgg atgcggaact ggacttagat cataaaatgt aatcgtacct gaacttttgg
     5821 tatctgcttg gactggctct tgccagctga agaacagcaa acttgcaata atccctccca
     5881 ataagatcga tgttttcaac gaaatcacct catcatgttg tttctccttt tttctttttc
     5941 ttgtggaccc acaaaaacag caaaatcaac cataacacta gaactgcagc aaccaatcct
     6001 gcataaagcc atgggaaaga ttgttcttgt actgcaaaaa cggaattttc atttactttt
     6061 tgtgcttttt cttttttgat cgtaaagttt tgtgtaaatc gccattcatt actgtcgcct
     6121 gattttgcta tgatttctgc gcggtaatcg ccggcttttg ctttatcacc agctaaatca
     6181 agattcaagt gaaaattcga atttggtgca actaaaaatt cattagacgt attttcaata
     6241 taaagctgat tttcataaaa gatcttgctc gtaaatatca aattgctgat aatcctaggc
     6301 tgaggatttt ggaaacgaag ctgaacagtt ggctttgcgt tgacctcgtc caaatctcct
     6361 gataaaagtt tcagatccgg ttctatcgtc tcttgacttt cttgtaaaac gactgcgaga
     6421 ttataagcaa tacgattttt gatttgtgct ccttctgtag cattttctgt ttcagcttct
     6481 tctttaggtg aaacatgaat acccgctaat acacgtccct taaagctttt tgctgggatt
     6541 ttcacttcta taggtatagt tgtttctgca tgtgctgata cattgatttc ctttttcgca
     6601 atgagcaaaa cgtcacggac atcaaacggc aatgtctcat ctaacgttgg gtttttttga
     6661 ctataatcga tcgttccacc atcactcgtt accgccggat tgacagagac ctcaaatgtt
     6721 cgttcttcat ctgaagaatt gctgataatt acttctagtg tctgattttg ttccggtgcc
     6781 actaataaat caaaataacc gattgcttta tctatttggt tctctggaaa taccggtttg
     6841 attccaaagt ctcctgctga atcctttgct tggctgattg acggaaaaat gaaaaataga
     6901 cttaaaatag aaaaaaacga aacaaacagt cgtttacttt gattcattgt ccaccacatc
     6961 ctttcaacaa caactgcggt aaaaaatcct tcacctatag tattttcacc gcagtttgtt
     7021 gaatttattt catactctat gctactgggc cagccacgag gttccaagtc aaatcagccg
     7081 tgaatgttcc agctgctgct ttcggcactt tcagtgtgat atttgctgga tcataaacag
     7141 aaagccatgt tcccagacct tgatcttttg ctgctgtaaa tattggtgtc gctgcacttg
     7201 ctttactcaa cccagttaca gtttgactag ttggtccagt ggattcgtta tctgacgtac
     7261 ttttaacagt ggatgttcca aaatctaatt cagcgccttg caaaacttgt gaaccgttta
     7321 caaaatctga aaccgaaact gtcacgttcc acccttgtgc ttggccatct gcacctacac
     7381 ctcgacgatc tgatacttgg agataaggtg tatcgttttt tgaagcgtct acttgatacg
     7441 tttttgttcc gctttcaatc tcgtgtgtac caaatggcaa ttcaggaact acatccaatg
     7501 ttaaagaacc tgtctggcca gttgctggat tttccggatc tgctggctct actggttttg
     7561 aaggatcagt agggttaaga ggctcaacgg tatttgttgg ttcagtaaag gtaatatctc
     7621 cttttgaagt cgcttgaccc tcagcaaaag ctggtgttgc caagccgcct agaagaagtg
     7681 ttgaaagtaa taatgtccca atcatgttct ttttcataat aaatcactcc tttttcttag
     7741 tgacctaagt atagtagaga cagtatgcaa aaaatcctca tgaattcatc cgcttacttg
     7801 aaattatgac cctttttgta taaaagagaa aaaaacaata aaatcgttta catgaatatt
     7861 cttccttttt tattaagtat gaaaaaatat ttcaatttta ttttttcgtt tcaaattatt
     7921 ctattagtga catccttgaa ctataaaaat aggagtacgg catcatctgc cgcactccta
     7981 tttttttcgt catttatttc ttaaataaaa cgattctcac gcaattttat ttttttgata
     8041 tcttctactg tttgtgttcc agctaattgc atgaccattt ccaattctgt tttaaagaaa
     8101 tcaaacactt gatggactcc tgtacttcct cctaaagaca atccataaat aacgggtctg
     8161 ccaatagcaa caagatcggc acctgatgcg attgctttga aaacatgttg tccacgtcgt
     8221 acaccgctgt caaacacgat tggtacacgt ttatctactg cttctgccac atattgcaac
     8281 gaatcaaacg cagcaggccc cccatctaat tgacggccac catggttaga tacccaaatt
     8341 ccaccagcac cagattctaa tgatcgatag acatcttctt ctgattgaac acctttcacg
     8401 tagactggca aatcagaatg agcagcgata aattctacat cttttggact aagtttttgt
     8461 ttggaagatt tatatacagc atccatcgtt tggcctactc ctgattgata agcctgaacg
     8521 atcggcattg gcagcgggaa ggtaaacccg tttctgcggt ctgtttcacg gtttccgcca
     8581 actgttgcat ccgctgtcaa tacgattgct tttgctccat tacgcttggc cacttcaaga
     8641 atatccaaat tgattccatt atctttgctc atataaaatt gaaaccattg cggtgctttc
     8701 tccccgcctg cttctcttat ctcttctaaa gtacaagaag cgtaagaact tgctgtatag
     8761 atcgtcccaa aatctgctac accttttgca gaagctttct ccgccttcac atgagctaat
     8821 ccatgagcag caactggtgc catgatgatt ggtgcagtca gcatttcttc gtcaaaatgt
     8881 gtagttgtat cgggtaattc tacatctctt aatacatgag gaataatcaa acgatgattg
     8941 aatgcccgtt cattttcttg ataagtaaat aagtcgccag caccactgct gatgtaacca
     9001 taaccaccag ctggaatcac ttgtgaagcc gcgatttcta aatcttccat attgataaaa
     9061 tctactattc cttcatttgt acctgcttga tatgtttttt ccatctcaat gccttctctc
     9121 tataaaattt gaatgatgaa caatatgata gcgtttacac tttttatatt actcttcgac
     9181 attgatacag gcaaataatc tgtttacatt tcagccattc cagatgtcac tactctggaa
     9241 gaaatgattg taataaaaat agataaaaaa ccgatgggac tttgacaaaa gtagtgtcaa
     9301 agtccctttt tttgaataaa cggtgttcaa aagccatttt ctcggtatta agtcaaattt
     9361 atgcaaaaat gtggaaagcc attttcacaa aatttttctt actactcgaa actaagcgac
     9421 tttttcacaa cctctcgata aaacggtgtt caatcagctg accctcggta ttagctcaaa
     9481 aaatggcaaa atatgaggaa ctattttgcc atttgtctct ctaatactca ggtcagaacg
     9541 ttgacttcac agtctcttcc ttaaacggtg ttcaaaaact tgcatctgcg gtaataagcc
     9601 caaccaaacg aaaaatagga ggagctattt ttgtttggct gttcttatta cttgatgcag
     9661 ggcaggtttt tcacgacctc tgattcacgg tgtttctttt tcatcaacat ttttacgaaa
     9721 tactgtggaa gactattgaa ataagtgtgg attgtttctt tgtgtttcaa tcgtttcttg
     9781 ccattcttca tttaattttg gattaccgtt ttcttccttg aactcataag gtcctaattc
     9841 aaaaatccca gacagtattt gattcacgat ttcatatagt tcagtgtgat cagacacatg
     9901 attcttgaag atcatctcat tatcaccacg tttgaacctc acattcgggc tctcatgatg
     9961 aacctctatg catttttctc ctttttttaa ctgacggact tctgaaaaca tctcataaat
    10021 atcttctcca gtaaaacctt cagcaagcgc cagactgtcc acaagaccgc ctactgtcag
    10081 ttcagagatt attgcttgat tggaatctaa ttttaatgta tagcttgtcg cttctactac
    10141 gccacatgca ttgcaaccgc cttctgatag ttcgcttttt ctaatcaatt gaacttcttt
    10201 cattttctct tcctcctctt aattttctac ttatgtattt ttatcacctg acataggtga
    10261 ctcctatcaa cgctatcatg tgcttttttc ctcagaaatc ctatttatcc attccatcta
    10321 tcccttattc ttctactttt ctttctctag catataagag atacgcatag tattcaagca
    10381 aggattgtcg atataaaaag aacaaatact gct
//
LOCUS       DACDMM010000088        10384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDMM010000088 DACDMM010000000
VERSION     DACDMM010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.089"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IWD16_001821"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IWD16_001821"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9088835.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="IWD16_001822"
     CDS             2714..2938
                     /locus_tag="IWD16_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088836.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            2951..3454
                     /locus_tag="IWD16_001823"
     CDS             2951..3454
                     /locus_tag="IWD16_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9088837.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            3514..3903
                     /locus_tag="IWD16_001824"
     CDS             3514..3903
                     /locus_tag="IWD16_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9088838.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            3890..6337
                     /locus_tag="IWD16_001825"
     CDS             3890..6337
                     /locus_tag="IWD16_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9088839.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            6342..8465
                     /locus_tag="IWD16_001826"
     CDS             6342..8465
                     /locus_tag="IWD16_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9088840.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            8462..9466
                     /locus_tag="IWD16_001827"
     CDS             8462..9466
                     /locus_tag="IWD16_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9088841.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            9485..>10384
                     /locus_tag="IWD16_001828"
     CDS             9485..>10384
                     /locus_tag="IWD16_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9088842.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIV"
BASE COUNT         3497 a         1698 c         2291 g         2898 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 ccaatcctta gtcttactgg caattgtggt cattggtttg tttttaggat ttaagcggga
     2821 gttttccaaa ctgattggct ttttggtgat tgctttgatt gcggtcggat tggtctttaa
     2881 tgcttctggg gtgaaggatg tcttactcag tttgtttaac cgaattgtcg gtgcgtaagg
     2941 gggaagcacg atggaacaga tgcgtgttta tattgcgaac ctcgggaaat ataacgaagg
     3001 tgaattggtg ggggcgtggt ttaccccacc tgtcgatttt gatgaagtga aagaacgaat
     3061 tgggttaaat gacgaatacg aggaatatgc aattcatgat tatgaacttc cctttgagat
     3121 tgatgagtat acgccaattg aggaaattaa tcggttgtgt ggcttagcgg aggaacttga
     3181 aggaacgcca gttggtgaag ttgcttctga gattcaacat gctttcttca attcttttga
     3241 ggagatggta gagcatgtgg atgatattat ctattatcca gattgcgatg atatgagtga
     3301 tgtagcatat ttcttgattg tagaagctgg tgattttgga gaagtaccag aacacttgaa
     3361 gacgtatatt gattttgatg cgtgtggtag agacttggag attggaggca attatttggt
     3421 aacgagtcga ggtgtttttg agtatccata ttaacaaaat tattgaaggg tagttgattt
     3481 ctaaactatc cttcttttta aaggaatgat ttgatgaaga aaataaaaag ctacaccagt
     3541 atttgggcag ttgaaaaggt catttatgca attaatgact ttcaactgcc ttttcctgtg
     3601 acttttaacc agatggcttg gtttgtttta tccttgctgt ttgtcatcgt atttgcccat
     3661 gtgcctccac tatctatgat agagggggcc tttttaaaat acttaggtat accggtggca
     3721 gttacttggt tcatgtcgca aaaaacgttt gacggtaaga aaccattggg atttttacga
     3781 tcttttatta gctatcactt acgatttaag gtcactttcg caggaaagaa agtcaaagaa
     3841 cagaagaaac gatgggacga acccattact ctggtaagga gtgtgaacta tgtacccgat
     3901 taagtatatt gaaaataatc ttgtctttaa tcaagaggga gaatgttttg cctactatga
     3961 gttaatacct tacaattact cctttttgtc acctgaacag aaatatcaag tccacgataa
     4021 ctttcgtcaa ttgattgcgc aaaatcgtga aggcaagatt catgccttac aaattgccac
     4081 agaaagtagt attcgtgcca cgcaagaacg atcaaaaaag gaaattaccg ggcgattaac
     4141 agaagtagcg aaacaacgaa ttgatttaca gacagaggca ttagtatcca tgattggcga
     4201 tagtcagatt gattatcgct tttttattgg cttcaaactg attgcgacag atgaagaggt
     4261 caatctaaag aatctcaaga aatctttctt ctccggcttt caagaatttg tctatggagt
     4321 gaatcatcat ttgatgggcg attttgtttc cttatccaat gaagaaattc gccgatatac
     4381 caagcttgaa aaactaatgg aaagtaaatt ggctcgaaga tttaaagtca gacgagtcac
     4441 accgagtgat ctgatgtatt tgattgaaca tatctatggc gaaaagggta caccatttga
     4501 agaatatgaa tttcagctac caaagaaaaa gctgaagtcg gaaactttgg tgaaacgtta
     4561 cgacttatta cgtcccagtc gttgtttgat tgaagaaaag ccccggtact tatgcatgga
     4621 acatgagaat catgaatcat atgtagcgta cttaacaatt aatacaattg tgggggaaat
     4681 ggagtttccc tcctcagaac ttttctatta ccagcaacaa caatttacat tcccaattga
     4741 cactagtatg aatgtggaga ttgtgacgaa caaaaaggca cttgctaccg ttcgcaataa
     4801 gaaaaaggaa ttaaaagact tggacaacca tgcctatcaa tctgacaatg aaacaaattc
     4861 caatgtctta gatgcattgg attccgtaga tgaattggaa actaccttgg atcagtccaa
     4921 agaatccatg tacaaactga gctatgtggt tcgagtaagt gccgaatcag tagatgaact
     4981 aaagcgccgg tgtgatgaag tgctagattt ttatgatgat accaatgtaa aactagtccg
     5041 gccttttggc gatatgatgg ggctacatga agaattctta ccttctagta agcgatacat
     5101 gaatgattat atccagtatg ttacgtctga ttttctcgcc ggacttggtt ttggcgccac
     5161 tcagatgtta ggggagttgg aaggcattta ctttggttat aacgtagata cggggcgcaa
     5221 tgtttacctg aaaccggcat tggcctcaca aggggtcaag gattcagtta ccaatgcgct
     5281 agcagcggca ttcctcggtt cactcggtgg cggaaagtcc tttagtaaca atctcttagt
     5341 ttattacgcc gtcctttttg gtggacaagc tgtgattgtc gatccaaaag gggaacgggg
     5401 tggctggaaa gaaaccttgc cagaaattgc ggatgaaatc aatattctca acttaactag
     5461 taagccaaaa aatcaaggat tacttgatcc ttatgtgatt atggagaaaa agaaggattc
     5521 tgaaagtcta gcaattgata ttctgacctt tttaacagga atttctagtc gagatgggga
     5581 gaagtttcca gtgttgcgta aggccattcg gacggttacc caaagcaatc aacggggatt
     5641 attacaagta attaatgagc ttcgaaaaga aggctcacca gttgcggaaa atatcgcgga
     5701 tcacatcgag tcctttaccg attatgattt tgcccatttg cttttctcag atggttcgat
     5761 tacccagtcg attagtttag ataggcagtt aaatatcatt caagtagcgg atttggtttt
     5821 accagatgca gaaaccactt ttgaagaata taccaccatg gaattgctta gtgtttccat
     5881 gctaatcgtc atttccactt ttgccttgga ctttatccat agtaatcgag aaatcttcaa
     5941 aattgtagac ttagatgagg cgtggagttt tcttcaagta gcgcaaggga aaacattatc
     6001 gaataagctc gttcgtgctg ggcgggctat gaatgccggg gtttactttg tcacgcaaaa
     6061 tagtgatgat ttactggatg aacgcttgaa gaataatatc gggttgaagt ttgcgtttcg
     6121 cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggcttgg atcgagaaga
     6181 tgaaagcaat caaaaacgcc tacgagattt ggaaaatggc caatgcttga tgcaggattt
     6241 gtatggtcgg gtaggcgtgg ttcaggtaca tccagtcttt gaagaactct tccatgcctt
     6301 tgatacccgt ccacctgttc aagagagaat ggagtgaggg gatgaaaaag aaactttttc
     6361 ggattgtggg aatcgttttt attagcatac tcactctgat agttctattg agtttagttg
     6421 gtacggtagc cgaggcgaca gggctagttg atgatacagt tgaagcagga aatctatatt
     6481 ccaaatattc actgaacaac tatcaactag atttctttgt ggatagctct tgggattggt
     6541 tgccttggaa ttggggtgat ggcttaggga aaagcgtgat gtatggtctt tatgccatta
     6601 ctaatttcat ttggacggtg agtttgtatt tgtcgaacgc cacgggttat gtggttcaag
     6661 aggcttacaa attagatttc atttcggata cggctgaaag tattggaaag aatatccaaa
     6721 cattggctgg tattactgaa aatggactcc agacctccgg attttatttt gggtttctat
     6781 tattgatgat tttagctctg ggggtgtatg tggcgtatac cggattatta aaacgagaaa
     6841 ccacgaaagc agttcgagcc gtgattaatt ttgtgatgat ttttctacta tcgggttcgt
     6901 tcattgctta tgcgcctacc tatatcacaa aaatcaatga ctttagctca gatgttagtg
     6961 aagcggccct tacccttgga accgagatcg ttgtacccaa ttcagaaagc caaggaaaag
     7021 atagtgtgga tttgattcgg gatagtttgt tttcgattca agtccaacaa ccttggttac
     7081 tactgcaatt tgatgattcc aacatagaag aaattggtga agaccgggtc aacaagattc
     7141 tatctgtgag tccagatgaa aacaaaggca aagatcggga agaagcggtc aaggcagaga
     7201 ttgaagacaa tgacaatgcg aatctcagta ttaccaagac catgaaccgt ttagggaccg
     7261 tggtcttttt ggtattgttc aatattggca tttccttctt tgtttttctt ctaacaggga
     7321 ttatgttgtt ctcgcaaatt ctcttcatta tctttgcgat gtttctgcca attagtttct
     7381 tattgtccat gttgccgact tatgaaagtt taggaaagaa ggcgattatt cgtttattca
     7441 atacgattat gatgcgagcg ggtgttactt tggtgattac gactgcattt agcatctcca
     7501 cgatgttttt caatatttcg gcaacctatc catttttcat ggttgctttt ctgcaaattg
     7561 tgacctttgc cggtatctat ttcaaattgg gcgacattat gagtatgttc aacctccaga
     7621 gtaatgatag ccagtcaatg ggaagacgcg tcatgcgaaa accgcaaatg ttaatgaatc
     7681 gaaaattgcg gcaactcaat cgaaatgtgg gacgagcttt ggcttttggt ggtggtgcag
     7741 cagtcggaaa taagttggca aaggaacaat caaaatccaa atttaagcca tccggttctt
     7801 ctctacgaaa gaattcacga ttaccgaacg atcacgaaat actatctgac ttaactaagg
     7861 agaatccagt ttcaaataat aagaaacaat cccgaatgaa tttggctggg agaaaaatcg
     7921 gcaaagtttt ggatacccaa gctttggtga aagataagac taaacaagta aaggatcagg
     7981 taaggaatac gccaacgaat ttgaaataca atctccataa aggaatcgaa aagtcgaaaa
     8041 aagcaccaga agaattcaag cgcggactgt tccaagaaaa agcggatcga gtggatttgc
     8101 gagaaaaaca acgacaacga agaaatgcga aaatggacga aaaacgcaag atactgagtg
     8161 aagcaacaaa tcgtcaggga aaaagaaggg cgaatattcc aatagataaa gcagctcaac
     8221 ctcagaaaga tatgatgctg aaaaaagaag caccaaagcg gattgtgaga gcaaatctga
     8281 atcccgaaat caaacgaaaa ctaactagtc aagaaatcat ttcaaaaggg aaaagccaat
     8341 cagcaagtaa gaaaaattct ttccaacaag tgaaacagag gtccactctg ccaaagcaga
     8401 ccaatcaaaa agtacaaaaa agaatgaacc atcgtccaaa atcaaggtca ggtgagaaaa
     8461 aatgaacttg aagaaagtgg gtgttatttc attaactttt ctgaccatct cttttatggg
     8521 tattttgatg tgtgtgggtt tgttgtttgg tgaagacagt gagggaaatg gtagttctgg
     8581 cacttctcct ggcggaacta gtgtctcaga agaagtattg aaacaccgtg caatggtgga
     8641 aaagtatgcc aaagaatcgg atataacaga gtatattcca attcttttgg cgattattca
     8701 agtggaatct ggtggcacaa tggaagacgt tatgcaaagt tctgagtcag ccggattacc
     8761 acctaattca ttaagtaccg aggcatccat caaacaaggc tgtctctatt ttgcgtcttt
     8821 ggttaagcgt tccaaagaac tagggtgtga tcaagatagt attattcagg cctataacta
     8881 tggtggcggg tatctagatc atgtcgggaa aaacgggaag aagcatagtt ttgctttagc
     8941 tgaatctttt tcaaaggaaa aatcaggtgg tcaaaaagtc gattacccca acccaatcgc
     9001 gattaaggaa aatggtgggt ggcggtataa ttatggcaat atgttttact gtttattagt
     9061 gaagcaatac ctaactacaa ctcagtttga tgacaaaata gtccaagaaa tttttgatga
     9121 ggcatttaag tatgaaggaa cggcctatgt gtttggtggc tcaagtccag aaacggggtt
     9181 tgattgtagt ggcttgaccc aatggtgtta tgccaaagtt gacctgaaac ttcctcgggt
     9241 ggctcaggat caatatgatg ccatgactca tattgatcta aaagatgcca aaccaggtga
     9301 tttagttttc tttcattcaa cctatgatgc cggtacttat gtgacccatg tgggcattta
     9361 cgcaggggag aatcgcatgt ttcatgcagg agatccagtg gggtggacga atctaacgga
     9421 aaactattgg cggcagcatc ttatcggagc tggccgatac aaataagaga aaagaggaaa
     9481 aagaatgaag ataaaaattg aacggaatca aaaggaaaag agcccaaaaa agaaaaaaga
     9541 acgagttgta tccgtgggta agcatcgaaa aatggtgctt gccctttggc ttttattgtc
     9601 ttgtagtctt gcttttggcg tttataaaaa tttcaccgcc attgatcaac atacaactca
     9661 tgaaaaagtg gttataaaag agaaggtagt gaatacttct ggcgttgaaa gttttacaga
     9721 ggattttgcg aaagaatatt tttcatggaa aaataacaaa gaagcaattg aaaagagaat
     9781 gagtaatctc gaacagtacc ttacagaaaa cggtctcgct ttaatccaag atatggttcg
     9841 agcagatatt cccacgagtt ccgaagttca atctgtaaaa attctagatg ttgaaaaaca
     9901 ctcggaagaa tttgtcgttt catttttagt ggagcaacat attacagagg gaaaaaagac
     9961 gcaatcaatt tcttctgcat atcgagtgac aatctttgaa gatgaaaaca ggaatcattt
    10021 agtaactagt ttgccaacaa tgattggaaa gccagacaag gcaaaatatg aagcaaaaca
    10081 agtagaaaac gattcagaaa ttgatgccaa aacaacggaa gaaatcaccg attttttaga
    10141 aacatttttt aaactttatc catcagcttc tgaaaaagaa ttggagtatt atgttgaaaa
    10201 tggtgcgatg cgaccaatca atattgactt aaagttttcg gagttagaga atccagttta
    10261 tctacaaaac ggaaaaaatt ttctagtgag tacaaagata aaataccttg ataatgtatc
    10321 aaaaaccact agctactttc agtacaaatt agtattacaa aaaaatggaa actggaaaat
    10381 cgtt
//
LOCUS       DACDMM010000089        10375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDMM010000089 DACDMM010000000
VERSION     DACDMM010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.090"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            256..531
                     /locus_tag="IWD16_001829"
     CDS             256..531
                     /locus_tag="IWD16_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088843.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="IWD16_001830"
     CDS             544..2616
                     /locus_tag="IWD16_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9088844.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="IWD16_001831"
     CDS             2781..4667
                     /locus_tag="IWD16_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9088845.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="IWD16_001832"
     CDS             4679..5626
                     /locus_tag="IWD16_001832"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9088846.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="IWD16_001833"
     CDS             5646..6173
                     /locus_tag="IWD16_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9088847.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="IWD16_001834"
     CDS             complement(6236..6889)
                     /locus_tag="IWD16_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9088848.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="IWD16_001835"
     CDS             7021..7863
                     /locus_tag="IWD16_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9088849.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="IWD16_001836"
     CDS             8021..8917
                     /locus_tag="IWD16_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9088850.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="IWD16_001837"
     CDS             8920..9633
                     /locus_tag="IWD16_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9088851.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="IWD16_001838"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="IWD16_001838"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9088852.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..>10375
                     /locus_tag="IWD16_001839"
     CDS             10168..>10375
                     /locus_tag="IWD16_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9088853.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYL"
BASE COUNT         3595 a         1772 c         2151 g         2857 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttag
//
LOCUS       DACDMM010000090        10231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDMM010000090 DACDMM010000000
VERSION     DACDMM010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.091"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..626)
                     /locus_tag="IWD16_001840"
     CDS             complement(<1..626)
                     /locus_tag="IWD16_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9088854.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            899..3370
                     /locus_tag="IWD16_001841"
     CDS             899..3370
                     /locus_tag="IWD16_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9088855.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3473..5209)
                     /locus_tag="IWD16_001842"
     CDS             complement(3473..5209)
                     /locus_tag="IWD16_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP9088856.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5206..5910)
                     /locus_tag="IWD16_001843"
     CDS             complement(5206..5910)
                     /locus_tag="IWD16_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9088857.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6041..6544
                     /locus_tag="IWD16_001844"
     CDS             6041..6544
                     /locus_tag="IWD16_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP9088858.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6504..6842
                     /locus_tag="IWD16_001845"
     CDS             6504..6842
                     /locus_tag="IWD16_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP9088859.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6863..8281
                     /gene="ffh"
                     /locus_tag="IWD16_001846"
     CDS             6863..8281
                     /gene="ffh"
                     /locus_tag="IWD16_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP9088860.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8393..8971
                     /locus_tag="IWD16_001847"
     CDS             8393..8971
                     /locus_tag="IWD16_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP9088861.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8975..9496
                     /locus_tag="IWD16_001848"
     CDS             8975..9496
                     /locus_tag="IWD16_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9088862.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9575..10129)
                     /locus_tag="IWD16_001849"
     CDS             complement(9575..10129)
                     /locus_tag="IWD16_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9088863.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         3304 a         1895 c         2112 g         2920 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagaa
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      781 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      841 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      901 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      961 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     1021 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     1081 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1141 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1201 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1261 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1321 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1381 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1441 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1501 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1561 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1621 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1681 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1741 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1801 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1861 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1921 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1981 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     2041 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     2101 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2161 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2221 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2281 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2341 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2401 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2461 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2521 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2581 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2641 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2701 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2761 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2821 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2881 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2941 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     3001 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     3061 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3121 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3181 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3241 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3301 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3361 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3421 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3481 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3541 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3601 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3661 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3721 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3781 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3841 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3901 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3961 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     4021 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     4081 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4141 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4201 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4261 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4321 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4381 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4441 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4501 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4561 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4621 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4681 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4741 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4801 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4861 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4921 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4981 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     5041 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     5101 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5161 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5221 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5281 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5341 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5401 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5461 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5521 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5581 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5641 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5701 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5761 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5821 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5881 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5941 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     6001 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     6061 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6121 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6181 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6241 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6301 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6361 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6421 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6481 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6541 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6601 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6661 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6721 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6781 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6841 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6901 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6961 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     7021 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     7081 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7141 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7201 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7261 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7321 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7381 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7441 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7501 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7561 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7621 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7681 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7741 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7801 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7861 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7921 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7981 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     8041 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     8101 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8161 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8221 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8281 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8341 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8401 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8461 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8521 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8581 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8641 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8701 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8761 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8821 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8881 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8941 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     9001 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     9061 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9121 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9181 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9241 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9301 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9361 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9421 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9481 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9541 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9601 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9661 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9721 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9781 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9841 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9901 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9961 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
    10021 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
    10081 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10141 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10201 ttactgacaa aagctacata gaagatagat t
//
LOCUS       DACDMM010000091        10210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDMM010000091 DACDMM010000000
VERSION     DACDMM010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.092"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(371..883)
                     /locus_tag="IWD16_001850"
     CDS             complement(371..883)
                     /locus_tag="IWD16_001850"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP9088864.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(897..3194)
                     /gene="recJ"
                     /locus_tag="IWD16_001851"
     CDS             complement(897..3194)
                     /gene="recJ"
                     /locus_tag="IWD16_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP9088865.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3336..3770)
                     /locus_tag="IWD16_001852"
     CDS             complement(3336..3770)
                     /locus_tag="IWD16_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP9088866.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3780..4568)
                     /locus_tag="IWD16_001853"
     CDS             complement(3780..4568)
                     /locus_tag="IWD16_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9088867.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4565..5506)
                     /locus_tag="IWD16_001854"
     CDS             complement(4565..5506)
                     /locus_tag="IWD16_001854"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP9088868.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5683..6270)
                     /locus_tag="IWD16_001855"
     CDS             complement(5683..6270)
                     /locus_tag="IWD16_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP9088869.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6491..7804)
                     /gene="obgE"
                     /locus_tag="IWD16_001856"
     CDS             complement(6491..7804)
                     /gene="obgE"
                     /locus_tag="IWD16_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP9088870.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8092..9102
                     /gene="gap"
                     /locus_tag="IWD16_001857"
     CDS             8092..9102
                     /gene="gap"
                     /locus_tag="IWD16_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9088871.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9187..10122)
                     /locus_tag="IWD16_001858"
     CDS             complement(9187..10122)
                     /locus_tag="IWD16_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9088872.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2816 a         2331 c         1769 g         3294 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaacttttac gggggtgtga caaaaatcgg tcacatcctt tttttcgaat aaacggtgtt
      181 ctaaagccag tttttcggta ttaagtcaaa ttgcggaaaa agtgattttt taaacttcta
      241 ctggtctttg cttgtttcca tttataaaaa aatagcgacc ctcaaaagtc agatagagaa
      301 tctgactttt gagggtcgct ttaaaatcgc ttctttaaga ataacatcaa gaatagcttc
      361 ttttttctgc ttaatattcc attaatgtaa tgatgtcata tcctttgatt ttgtcgcgtc
      421 catggagatc catcaattcg atcaaaaatg cgcagcctac gactacaccg cctaattgtt
      481 cgatcagttc gatcgtcgct ttgattgttc ctccagtagc tagcaaatcg tcacaaatca
      541 gcacacgttg acctggttta atggcatcct tatgcaatgt caatgtatct gttccatatt
      601 ctaatccata agtcacttcg atggtttctc gaggtaactt cccttttttg cggactgggg
      661 caaaaccgac tcctaactca taagcaacag gacatcctac gatgaatcca cgggcttctg
      721 gcccaactac catatcaatt cgtttttctt ttgcataatt tacgatttgt ttagtagctt
      781 cccgataagc gtcaccatct gccatcagtg gggaaatatc tcgaaacaca attccttttg
      841 acggatagtc gggaatactg gcaatataat cttttaaatc cactctttgt ttcctcctag
      901 gttgttaacc atttctttat ggtaggaata tcgctcaaaa gcaaaaattc ctcaactttg
      961 attttattga gccgtcgctg gtataatctg ctctcagtca aaggatgact ttctgggcta
     1021 gcgacttttt gtaaaacacc gttctctatt gtaacaaatt tcaattcaaa aaacacctgt
     1081 atcataaaaa ttaataattt ttcagggata cgcaaatatt gagctattgc ttttatttta
     1141 tgacgtatat caattcgttc ttgagaatgg atcaatttaa acaattttgc gtactgttct
     1201 cttgtgccta ccccattcaa ataggcctca tcaggtgaaa tccccatcag ataaacacga
     1261 ttgaatgaat aagaccgaaa aacttctttc agatccgcta aatcatctgg acaatccata
     1321 acaactagag agtttggttt tcttttttca attattgcct ttacagaatc tagtccttcg
     1381 aaaacaatcg cagcttcctg gataatctca ggcacgaatt tcaacgtcga agaatcaaat
     1441 gctaaataaa ggctaccttc cgacgttcgg ctctgttcac ggaaacgttt tgcccgccaa
     1501 tcaaatactt gcagaccacc cacagaaaaa tcagatacca tcagctgcgg tttcttacgc
     1561 ccgttccatt cattgatcga aagctttcct accacatcta cctgattatt cagcagttct
     1621 tcttcttgag taccaaaacc aaaagcgacc gcatcaagct gactcgatgc atcggaaagg
     1681 acaaatttca gatgctgttg gtttgctccg attcttttga tattttctgc ttggacttgt
     1741 cgaaataaaa actgaggcaa aggattatcc gtaccaaatg gcgccaatag tttcagctca
     1801 tcaattcgct ctactgttac ttcatttggc agcaatactt catcaattct caactcaggc
     1861 cctctcatca agtcaatttg atgatcgact atatattggt tcatcttctc ctgtagtata
     1921 gtgacatttt cacttggcat cgttaaaccg actgctgcat gatgtcctcc gaagtaagta
     1981 aataaatctc gcatctggtc aagcatttca aaaagattca aggcttcaat actacgtcca
     2041 gatcctttag ctgatccatc ttcttttaaa gtaaggacaa gcgttggttt acccgtttca
     2101 ttcattattt tcccagcaac aatgcccaaa acgccttcat gccatcctcg gtttaccaat
     2161 agatggatat gattctcctt attgaccatc gctaatgctt cttgtgttat ctgctctacg
     2221 attgcttttc tttcttcatt gatctcattc aatttttttg cttgaacgga tgcttcctct
     2281 tcatcaaacg tcgcaagtaa cgagacggca ggacgtggat cgcccatccg accgattgca
     2341 tttagtctgg gagcaatcga aaaaccgatc gttgtttcgt ctgcgtccct catttttacg
     2401 ccgctttctt caaatagcgc ctgaagtcct atccgttctg aatgatgaat ggcatcgatt
     2461 cctaatgaaa caaggattcg attctcatca gtcaatgaaa ctaaatcggc gatcgttcct
     2521 atcgctacta aatccaaaaa ttctgcaggc ggttcttcca ataatgcaga agctactttg
     2581 aatgctactc cgactcctgc caaatctgga aatggatagt gccccgctgg atgacgaggg
     2641 tgaacaatcg cataagcgtc tggtaaattc tcaggtagtt cgtgatgatc tgtaatgatc
     2701 acgtctacac cagcttgctg agcgtatgct accgcatcat ttcctgctac cccgttatca
     2761 acagtgatga tcaactggat tccttcttcg atcttttctt ggtataccga ttggtttggt
     2821 ccatacccat gctcaaatcg atttggcagg aatacttcta cttctgcacc aagtaattcc
     2881 agtgtttctt tcattaccgt tgcactggtg atcccatctg cgtcataatc accgtaaacg
     2941 aggatttttt ctccactgat tacagcttct tggattcggc tgattgcttt atccatatcg
     3001 tgtatcagat acggatcatg taattcttgt tcctttggat gtaaaaatcg gttgatatcc
     3061 ttttcctcat ggtaacctct ttgccacagt aacttaccga caaatggaga taatttcagt
     3121 tcacggatca tttggagaaa ggattcagaa ggctctgtct cgcttaactt ccaatcaaaa
     3181 tttgcttgtc tcactttctc actcctaact tagttattat agcaaaggaa atcagcaaaa
     3241 aaaagcagaa agaaagaata aaaacagatc agatccgcta gacagatgtt cagcaggacc
     3301 tgatcttttg tacgaccatt tatgaaatat ccctattagt caaaataatt gaagctttgt
     3361 ttgggcgatt gagttttttg ttcttccggc gctttcaatt gactgatttg tgcggttttc
     3421 gcttgtaact ccgcctgata agcagcttcc aactccgcac gttcgtttct aaactcgcgt
     3481 tgctgttctt cttttgcttc ttcaattttc ttttgaagtt ctgtttgata ttctgacatt
     3541 tcctgagtca gctgtttgat ttgctttttt tgctgccata gtgttgccga agcagtcaaa
     3601 aatacaatca gtgcaccaat cagcgcagaa ccgatgatca ctaagatcaa tggcgcagaa
     3661 aaggaagtga agccaaaact tacaggaaca tttttattgt tcaatacagc aaaaagaaca
     3721 ataatcaaaa ctaaaacaaa tccaagaatc acattgcttt gctttttcaa ttccttcacc
     3781 tactttttgt taaaaattcc gctggctaaa taatcaccaa tcgttggaaa cagcgtataa
     3841 aattttgatg caacttctaa aataaacggt tgattgattt cccgttttgg atgtctcata
     3901 ttgcggacga tcatttttgc caacttggca ggttctaaaa cgatttgtcc gactttttcc
     3961 aaatagcttc cgcttggatc agccttatcg aagaaatttg tctcgattgg acctggattc
     4021 actgtcgtaa cagctacgcc aagtggtttc aattctaatc gcaaagcgtt ggagaagcca
     4081 aggactgcaa atttcgtggc agaataaacc gttgattttg cagttgccat ttttccagcc
     4141 atagaggcga tattaatgat atgaccttgt cttctttctg ccatatcgat cgcaaatttt
     4201 tgtgtcaaaa ccatcattcc taagatgttg acttcaaaca tatcgtatgc ttttccaagg
     4261 tcgaatgtaa ggaagtcttc gaaaatccca aaacccgcat tgtttaccag aatatcgacg
     4321 cgcccgactt cttccatcac cttttcatat aattcgtcca cgctttcagg attgctgacg
     4381 tctaccttga aagcataagc ctctttgccg ctcaagcgtc tgcactcgtc tctgacacct
     4441 tctacaaatg ccattcttcg tgcgcaactt ataacgattg ctccttgctt tgctgcttca
     4501 taacagattt gtgcgcccag accgcctgaa ctacctgtta ctaaaacaac tttatcttct
     4561 aaattcatgc ctcttcctcc tcgcgaaatg gaatctcaat gctatcaaaa tctttcataa
     4621 tcgtcgtgtt ttcaaacact tcttgtgctt ctttttcaag ctctaaagct gcttttccta
     4681 aatatctcgc actgatatga gtaagtagca gccgttttgc ctgcgcttct tttgcaactt
     4741 cagcagcttg atgagtcgtg gaatggaaat agttgtgcgc cattcttgct tcatctttat
     4801 taaatgtgct ttcatgtacc agaatatcgc tattttccgc aagcacaacg gaattatgtg
     4861 tttttctcgt atcgcctaaa atagtcacga ttcgtccttt tttatctgga cctacgaaat
     4921 cttttccatt gatcgtccgc ccatcttcta gttggatcgt ttcaccttgc ttcagtttgc
     4981 cgtacagtgg tccagctgga atacctaatt cttttagacg atccacctgc aattctcctt
     5041 tatgatcatg ttcaaccaca cgataaccaa aactgtcgat cccatgattt agtttttttg
     5101 tataaacaga aaattgctga tcagtaaaaa tcggggctgt ttcatttagc tcaatgaatt
     5161 tcagcggata acttaaacgc gtttgcgaaa tacctaaaga gacttttatg tattcctcta
     5221 tcccttttgg cccgtaaatc tctaatggcg tatccccacc ttgaaatgac cggctgctga
     5281 tgagcccggg aagtccaaaa atatgatcgc cgtgtaaatg tgtaatgaaa attttaccga
     5341 tttttctcgg acgaatagtc gttcgcaaaa tctgcatttg cgtgccttcg ccacagtcga
     5401 acaaccaaac ttcattgcgc tcgtctaaaa gtttcaatgc tatacttgtc acatttctat
     5461 gttttgcagg aacacctgca cctgttccta aaaattgtaa ttccataatc tacctgactt
     5521 tctttaagtt cctctttaat tttagggtaa aaagaaggga taatcaaccg taagtcgaga
     5581 atgggttcaa attaaaaata acgttctaaa gcgatagcgt tttctttgaa aattactata
     5641 aaaacagcag cgaacccgtt tgagatcctc tgctgttttt tattaatttg aagatccaga
     5701 actttcattg tcgttggaag tttgcggatg tttgtccttc caagtctttt gtgcagcttc
     5761 tttcgcatga ttttcagctt ctttcgcatc atgcacatct ttttcgacat cttcataatc
     5821 caagcgagta atttctccgc ctaattccat cattaccaat tgattcagtg ggcgattgtc
     5881 ctcttcatca gcaatcaaga tcagtcctgt agctccttca ccgattttat ttcctacaaa
     5941 gtcgaacacg gtctgggcat cttttatttc tttggcatct tgtgtcgcac cgatcattcc
     6001 tccggcaaac catcctaata aaatccctag cggaccacct aatacaccaa ccaacattcc
     6061 gattaatcca cccgtagaag atttgttatt accggtaaag tcgataaaat cttctatttt
     6121 gaattgatgt gtcccattag gatcatggct gacaactgcc atctgttctc ctttaatcga
     6181 acgaacagca tgtaactttt tgatttctga aaatgcctga tatgcctggc tttctacttg
     6241 aaaatttaaa atgattactc gtttgctcat taaaaacgcc cctttcatac cttcttgtta
     6301 tttattttac ctttaattga aaaataagaa aagaaaagca ctcttttttt ccaataaaat
     6361 agggctgtga caaaagtcgt atcacagctc ctttttccga ataaacggcg ttcttgaagc
     6421 tgatcacttc tgtcacaacc tcccttaaat ctgaatctat attttttggt atttgttttt
     6481 atttgcttgc ctattcgaca aattcaaatt caaactcacc gatacgtaca aggtcgccat
     6541 ctttcgcacc gcgtgcccga agagcttcat ccacacccat accacgtaat tgacgagcaa
     6601 aacgcatcac tgtttcatca tgatcgaaat tcgtcatttg gaataatttt tcgatcttct
     6661 caccagataa gatccacgtt gcatctgaat cacgatcgat ttggaactca ggaccttctg
     6721 gattgaatcc atagtgtaca gtttcttctt ccagttcttc ttcgtacaat gggaattctg
     6781 gcgttacttc taacaaatct gctgtagcat tcaataacgc gtccaatcct tgtcgagtca
     6841 ctccagaaat tggaaatact gggatgtcat cagcaaattc atcttctttt tccttgttca
     6901 gctgttcttt gaatttcact agattttccg gtgcatccgg catatccatt ttgtttgcca
     6961 cgatgatttg cggacgctct agcaagcgta agttataagt agataattct ttgttgatgg
     7021 caagatagtc ttcataagga tctcgtcctt ccatcccact catatcgatc acatgtaaaa
     7081 ttactcgtgt tctttcgata tgacgaagga attgtgtacc cagcccaaca ccttgcgaag
     7141 ctccttcaat caagcctggc aagtctgctg cggcaaaact tctgccatca cttgtagtaa
     7201 ccattcctag attaggtacg agcgtcgtga aatgataagc gccgattttt ggacgagcag
     7261 atgaaataac ggatagtaaa gtcgatttcc cgacagatgg aaaacctaca agtccaacgt
     7321 ctgccaatac tttcaattct agttcgattt ttcgctcttg tcctggttca ccgttttcag
     7381 caatttccgg tgcaggattc cgaggagagg caaaacggat attccctcgt ccaccgcgtc
     7441 caccttttgc cacaactagt gtttgaccat tttcaattaa atctcctaaa agagcaccag
     7501 tctccgcatc tcggacagtg gttccttgtg gtactttaac ataggtatct tctgaaccac
     7561 gaccgtgcat ccctttactc atcccatttt cacctggctg cgctttgaaa tggcgattga
     7621 accgaaaatc catcaatgta cgtaaccctt catcgacgat caagatgaca tcgccgccac
     7681 gaccaccgtc tccgccagcc ggtccgccgt caggaacgta tttttcgcga cgaaatgcaa
     7741 ccattccgtc tccgcccttg ccagctttca catcgatcgt tacttgatct aaaaacatgg
     7801 acataattaa gtcctcctat tattcattta tttatttgac tgcctgaatt tccacaataa
     7861 acaaaagtct tacttatttt atcggtgaaa tctgattttg tctatcattt cagcaaaaag
     7921 attgtttacc ttcttctttt tttatcattt ggtaaggttt ccaacctttt ttctgctacc
     7981 aagtataatt ttttgacaaa atattctttg cgtagcagaa taacagtgta ttaattgtat
     8041 taattgagga tgccgtttca cagcacccct aataaaggag gaacttttga tatgacagta
     8101 aaagtaggta ttaacggatt tgggcgtatt ggccgtctgg ctttccgccg catcaaagaa
     8161 gtctcagatg atattgaagt agtagcaatc aacgacttaa caagtccaac tatgttagca
     8221 caattattgc aatttgactc tactcatggt acttatcctg gcacagttac tgcaacagaa
     8281 gactcaatcg tagtggatgg cgaagcaact cgtgtttatg cagaacccga cgcaagcaaa
     8341 atcccttggg caaaagaaaa cggtgtagat atcgtactag aatgtactgg tttttataca
     8401 tctgaagaaa aagcaaaagc gcatttagat gctggtgtca aacgagtagt tatttctgcc
     8461 ccagctggtg ctatgaaaac catcgtctat aacgtcaatg atgatacatt agatgaaaac
     8521 gacaaaatca tctcagcagg ttcatgtaca acaaactgtt tagcacctat ggcttatttc
     8581 ttaaataatg aatttgggat cgaagttggt acaatgacaa cagttcatgc ctacacttcc
     8641 acacaaatgc tcttagatgg acctgtaaaa ggcggtaatt tgcgtgctgc tcgttcagct
     8701 gctgacaata ctatcccaca ttctactggt gctgcgaaag caatcggctt agttatccct
     8761 gagctacaag gaaaattaca aggacacgca caacgtgttc cagtcgttga tggttcatta
     8821 actgaattgg tttcagtatt gaaaacaaaa gtaactgctg atcaagtaaa cgaagcaatc
     8881 aaaaaacaca cagttgacaa tccttcattc gggtatgatg accgtcaaat cgtttctagc
     8941 gatgtcattg gaacaactca aggttcgatc tttgacccaa cgcaaacaga agtaacaact
     9001 gctggtgatt tccaacttgt taaaactgtt gcatggtatg acaatgagta tggatttact
     9061 tgccaaatga ttcgcttact agaaaaattc gctaacttgt aatttgatag atcaatcgat
     9121 aaaagaagcc tttgactgga tccagtcaaa ggcttctttc ttttcatccc ctacttttaa
     9181 actcgttcaa tgaacatatt ggcttaaaaa ttgttggact acttgctgat atttttcagg
     9241 atctgtcttg tatgcttccg catgctcggc tccttttaca acatactttt cttttggtcc
     9301 cttcgttgca cgatagacct tatctaacat ttcaaatggt acaaatgtat cttcgtctcc
     9361 atgtataaag agcattgggc gctggttttt cttcaattgt tccactgcat ttgcttcacc
     9421 gaaaaagtag ccagcacgga cttttgtcac tagactcgtt acttgcataa gcggaaatgc
     9481 tggaagacta aacatatcgt ctagctgata agcaagctca tcatgaacgg aagaataacc
     9541 gcaatcttca atgattgctt tcacattatc tggtagtttt tctccgctag tcatcataac
     9601 tgttgctgct cccatgctga caccgtatag aacgatttct tgcgattttc cattgttttc
     9661 tagcactttg tttatccact gtacatagtc tttacgctct ggccatccaa atccaatgta
     9721 atccccttcg ctttttccat gtcctcttgc atcaggaaca aggacattgt aacccatatc
     9781 atgaaacatc tttgcgtaat tggtcatcgt ttcagcgttt cccatatagc catgagccac
     9841 aagagctgtt ttttcacttt tcgtttcagc aggaagataa atcgctgata atttcagacc
     9901 gtcttttgaa gtgatggtcc aatcgcttcg gttttctttt gaactaaacc atttatgtgc
     9961 atccgtttct ttggatgtct gtgttgtacc aggtgtatct tcgtctaaaa agtctttctc
    10021 agaaggcaca acagcataat tataaaaata attccctgca aacactaacg caacaatcag
    10081 agctaaagcg acgcctaaaa ggattttcat ccaaagtttc ataaatcatc catctcattt
    10141 ctcttttttc atataacctt gaattagtat aatcaaagaa tctctactat gcaatgttca
    10201 tttttataac
//
LOCUS       DACDMM010000092        10192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDMM010000092 DACDMM010000000
VERSION     DACDMM010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.093"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            223..1071
                     /locus_tag="IWD16_001859"
     CDS             223..1071
                     /locus_tag="IWD16_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP9088873.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            1216..2157
                     /locus_tag="IWD16_001860"
     CDS             1216..2157
                     /locus_tag="IWD16_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP9088874.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            2147..4318
                     /locus_tag="IWD16_001861"
                     /pseudo
     CDS             2147..4318
                     /locus_tag="IWD16_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(4430..6601)
                     /gene="copB"
                     /locus_tag="IWD16_001862"
     CDS             complement(4430..6601)
                     /gene="copB"
                     /locus_tag="IWD16_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP9088875.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(6621..8807)
                     /locus_tag="IWD16_001863"
     CDS             complement(6621..8807)
                     /locus_tag="IWD16_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP9088876.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(8807..9016)
                     /gene="copZ"
                     /locus_tag="IWD16_001864"
     CDS             complement(8807..9016)
                     /gene="copZ"
                     /locus_tag="IWD16_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP9088877.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(9029..9469)
                     /locus_tag="IWD16_001865"
     CDS             complement(9029..9469)
                     /locus_tag="IWD16_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP9088878.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(9543..10082)
                     /locus_tag="IWD16_001866"
     CDS             complement(9543..10082)
                     /locus_tag="IWD16_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9088879.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         2875 a         2162 c         1828 g         3327 t
ORIGIN      
        1 taatcgtatt ctcatttcaa aacgaatgta ctttaaaaaa acataataag atcactttgc
       61 ttgtatgatg tattcccttc cattacaatg aaataaatag cgattttatg agaaggtgat
      121 gtgaaaatgg aagaaaaaat agaagaatcc aaacaaaaaa tcgatcagtt gatataagaa
      181 ttagaaaaag cggtcatcag caaacgttca actatccaat tgatgatcgt ggccctatta
      241 gcaggtggtc atgtcttatt cgaagatatt cccggtgtag gtaaaacgtt actggtcaaa
      301 gcactagcaa aagctgtaca gggaaccttc agcagagtcc aatgtactcc tgatctgctg
      361 cctagtgata ttcttggttt ttccgtatac aattcacaat caaaagagtt tgaatttcgt
      421 ccgggaccga tctttaccac gatcttgtta gcagatgaga tcaatcgtac gactcctcgt
      481 actcagtcag cacttttaga agcaatggcc gagaatcatg caacgatcga caacaataca
      541 taccctctag acaatcattt ttttgtctta gctacgcaaa atccaatcga gtacgaagga
      601 acatatcctt tgccggaagc acagctcgat cgttttttat tccgactaca aatcggttat
      661 ccttcttttg atgacgaact tttgctactt ttggataaat tcgaaaaagt gttggataac
      721 ttaaatgaag tattgaacag ttatgaaata gaagaaataa aaacaaatgt ggcgaatgtc
      781 tttgtcagtc ccgaagtggc atcttatgcc ttgcagttag tgtcagcaac aagaagtcat
      841 gctgccatcc agcttggcat cagtccacga ggaagccttt cctttattca ggcagcaaaa
      901 gcttatgctt taatccatgg gagaaattac gtcacgccca aagatttgca ggatttagtt
      961 ccttacgtgt tttcccatcg cctgattttt tacgatcgtt cattgaagga agacgaaaag
     1021 aaacaaatca ttcatactct tgttgcacaa attcctattc ctgtgaggta gattgaggta
     1081 gacaatggaa aaaatcaaag attatttcat taattttgtg ttatttctgg tatatttcat
     1141 cattgtgctt tttgctcttg tattcaacaa ttctttaggc tggttcttgt tggccttttt
     1201 tactctttat gtatcatgga gcttgatttc attaagcagc tttctaaaga aactttcggt
     1261 tacttgtaca ttgcagccac tcgtttatcg caaaaaaagt atgcaggtta ttttttccat
     1321 agcaaaaaag agaacttttt ctttacctct ccctcgttta caaatcggct tcccagcaat
     1381 ctttgcagaa aaaaacaaga gattttgatt cttacaaata aaccgcaaga agtccaattg
     1441 ctttggcagc caaaagaaag aggatgccat gaaacattaa ctgtcatttt tacagcctat
     1501 gatagtctag gacttttcag aaaaagtgca aaaagagaga tctcttttcc tgtgactgtt
     1561 ctgcctgctt tttgtaagat tcaagcagaa aaattacaga gagttctgga aaaaaaacag
     1621 cagctgaacg cgtaccaaag aggtcttgat tttcgggaac atcgcgctta tcgtccaggt
     1681 gattctttca accgaatcga ttggaaattg tctgctcata ctcaagagtg gttataccgt
     1741 gaatacgatt atcaagaaga agaatgttct cctctttttt gcttttgggg tttgcccagt
     1801 ccaaaatttg aacctctgct agacttgtat ttttctttat ggcatttgag aaaagaaaag
     1861 cagccggaac ttctgatatt aggcaaccgc gcctttcatg gcaaagaccc cggtcatacc
     1921 tattttgctt ctttggaagc tggcgatgag aaagcttttt tcgcacactt gaaaaaatat
     1981 gcaaaaaaac aaatcgtcct ctttgttcct gagaattctc ctgaagtctc agaagctatt
     2041 gaaacattgt caaaagatcg aacggtttat cttgtttatt tcgtagaaaa acaactcatg
     2101 gtccaagaaa aagacaagat atgttcattg ccaggaggtg aatggattga cgattaaacg
     2161 ggaaaaatgg ctgcgtggcg tttactcctt tgtgatacta agtgtaggtg ctccgatatt
     2221 tgtagtagct aatcaattgc aaaacagcta ccttcttatc ctaactattg gtgtcctatg
     2281 ccttattgtc tgctgtattc ctaaaaaatg ggtacgtttg ctgctttctt tgccagtaat
     2341 gataggttgt ttgtttttat attttccttc gaagacattt tccatattat ggatcttaca
     2401 gtttttttcg tcagtcacag aggaattctt ccatctatcc caaggtaaac tttattattt
     2461 gccagaaaag acagcttttt ttctcataat gttgctgatc tatttattta ttacattgac
     2521 cgtggattac gactattggt acgcaccctt tctttctttg atggcttact tcatgatgct
     2581 taccgttttt cggaaaaaag atttactgta cgaaatgatt gcggtagtaa ctgttttgtt
     2641 cgtgtatctg gcagcaaggc aatttttctc catccggttg ttgtcgggaa acactcgatt
     2701 ccagtcattg actacagttc tgcttatttg tttcttaact tttagcgcta tcttgccttt
     2761 atatcttccg aaagtacacc aatctctcga ggaaacgagt gaaccgatcc gagaatatct
     2821 taatgatgaa gggctttatg acacactcca tgagtatcaa ttaaccggaa gtgcaagaac
     2881 aggcttcagt gagaatgatg aacagcttgg tggaccttta tatgataatg gcgaagttgt
     2941 ttttacagcg aaccaaaaag gaaatggcta ttggaagatc gaaaataaaa atcattacac
     3001 cggttctgga tggatagaag atcaggacgc acccatccaa ccaatccaag aattccctgt
     3061 tgacatcacg agctaccaaa ccgttataga agacaaagaa aacattacat tgaccctaaa
     3121 aaaacaagtt ttcttacctt atccttatgg aaatactacc ttgactgatt tttcattagc
     3181 tagtggacaa acgctttact atttgcctga aagccagcga ttcattcctg tcccgaatga
     3241 atcgacacgt tctgcaactt tacaaacaga aaggttagtt tttacgagag aagaattaaa
     3301 aagcagtcag ttaaaagctg atgatttaga aaatcagcag acaaatcttc aattgccaaa
     3361 aagtttgcct aaacgtattg aggacttggc ggtcgaattg accaaaaatg aagcaacttt
     3421 aattcgtaaa gtcgaagctg tctaacagta tttaaaaaca agcggaaatt tccgctattc
     3481 caaaacggat accgggcata ctccaaaaga acacgattat gtggattatt tcctctttga
     3541 ttcaaaagtc ggctattgtg acaacttttc aacttctatg gtcgtccttc ttcgaacact
     3601 tggtatccca gctcgctgga caaaaggttt tacaaatggg ctcagaacag gcacagataa
     3661 tcaagggaat gaggaataca gcattctcaa tagtcatgct cattcttggc cagaggtata
     3721 ctttcctgga tttggctggc ttccatttga accaacccct tcatttgcca atccaatggg
     3781 ttcacaacca gttgctgaaa caaccgaaac cgtccagcct gctatcgaac aaccaaatga
     3841 aagcgaaaat atggcagaaa ctatttccag tgagagatct gaggtcattc catcagaaga
     3901 aacaacactc acaccagctt ataaaatcgt cttattcagc cttggagtgt gtttatccgt
     3961 cttgttgttc tttctattgt tattcagaaa atctctgcgc tggacaagtt atcttatcta
     4021 tcttcatttt tttgcttcta ctaatacgat tgcatatcaa aaacttctta accctttgga
     4081 aacattggta ccgcgcaaac ccgcagaatc tttgcaggaa tatagcctgc gaatagaaca
     4141 agaattcaag ctagatctgc gtttcattcg attaactact caatatgaag cagaaatata
     4201 tggacgacaa gcagatccgg taaaattcga taaaaaaacc tatttagaaa taacgcatat
     4261 aataagaaaa atggtataca aaaaaattct gacaaaaggg agccgcttca aaaaataaga
     4321 taaaatttct ggagaatcta cttctggaat gccgagaacc agctgattga gtatcgatta
     4381 ctcgaaaaaa aggttgtgac acgttttttg tcacaacctt ttttcattat tatctgattt
     4441 tcaatgtcaa agcatttaga gctacaacta ccgtacttaa cgacatcaag acagcaccaa
     4501 ctgctggact taatatgata ccaattggcg caagaattcc ggctgctagc ggaattgcaa
     4561 gaatattgta acctgccccc caccagaggt tctggatcat tttctttcgc gtttgcttgg
     4621 ctagatcaag aaaatgcaaa atatcttttg gatcgctgtt tgtcaatacg acatccgcag
     4681 aatcgattgc gatatctgtc cctgcaccga ttgccatccc gattgaagca cgggctaaac
     4741 ttggcgcatc attgatccca tcacccacca tgattacttt tttcccttgg tcaaggtatt
     4801 tctggacaac agcttcttta tcatctggta ataaaccgcc ataatattct tcgatcccta
     4861 aataatctgc tactgcttgg gcagcgtttt tattgtcacc agtaagcatg acaggagtga
     4921 tttttctttc tttcaatgtt cggataaatt cttttgcttc tgtttttact acgtcaccta
     4981 aagcaacgat tgcagctaga tgattttcta aaatcaggaa gctgatggta tttccttgtt
     5041 cttggtaagg tttcaataat gcttgttcta cgtttaatcc gagacgctca acttcttttt
     5101 cattaactat tttcacttgt tgttttttga ctgttgcctc tagcccgact cctgaaagat
     5161 tttttagatt ttcagcttga taaggcttga tttcatgttc tttcaaataa ttcataatgc
     5221 caacagccaa tggatgattg gcattcgctt ccaaggctcc tagatattga aggatttcat
     5281 ttttattgaa cgcatcatca agtacttcga cacccgttac agtaaatttt ccttctgtta
     5341 gtgttcctgt tttgtccagc atgatcacat ctagatcatt ggcttgctcc agtgcattgc
     5401 ggtttttcaa aagtaatccg tttttagctg ctattgatgt ggaacgagca acaactaatg
     5461 ggatagctaa acctagagca tgaggacagg cgatgataaa gactgttacc atacgttcta
     5521 atgcattagg aagatctgtc aagaacaacc aggcgatgaa tgccaggata cccacaatta
     5581 atgcgacata aaacagccat tttgccactt tgtcagataa tgactctagc ttggattttt
     5641 ctccttgtgc ttttcgcacc atttccataa ccttcgctag ataaccattt tcaccagttc
     5701 cagttacttc gatttcgatc gttccatccc cattgataga accgccaatc actttgtcat
     5761 tggcttgttt tttgattcct cttgattcgc cagtcacagc tgattcgtca acaattgttt
     5821 cacctttcaa gattacacca tctgttggca ttttatcacc agaccggacg atcaaacgat
     5881 caccttcgtt tacttctttc aatgaaactg tctcttcttt tccatctttc gtcaagcgtt
     5941 tcactgattc tggcagtaat tctgctagtt tttgtaaagc attgctggca ttcgacacag
     6001 cattcatttc aatccagtga cctagtagca taataacgat caaagtagcc aattcccaga
     6061 agaaatccat gacgtgtgta tgtggattga gcagatttgc gataaacgaa tagacactat
     6121 aaatatatgc aactgtaatc cccatcgcga tcagtgtcat catagcagga cttttttgtt
     6181 tcaattccat cttagctcca cttaaaaatg gctgaccgcc atagataaat agtatagttg
     6241 ctaagatcaa tacgagccaa tcagaaccag gaaacgttac ttgaaatgga aattccattc
     6301 ccatcattgg tgaaaaaaga atgattggga tagctaggat aagcgatagc caaaattttt
     6361 gtttgaaatt ccccatgtgc atggaatgat ccatgccgcc gtgttcgtga tccatgtgca
     6421 tcgaatggtc catttgtttg ctgtttgcta tatctccatg gttatgtccc atgtgcatgg
     6481 aatgatccat ctgactatgg tcatgtttca tctccatctg tttttcatga gattctgtac
     6541 tttctttgtt tactacttct tttttatgct catgtttttt ctccatatcg ttcccattca
     6601 tttttactcc atcccttctc ttactttttc atgtgtcgat ttaagcacag ggagttcaac
     6661 aatacactga cggaactaaa cgccatcgct cctcctgcaa tgattggatt caaaaagcct
     6721 agtgcggcaa aaggaatacc aatcgtatta tagacaaaag cccagaaaag attttgtttg
     6781 atttttctca atgtcaacct agacaactcg attgtttgtg caatggatgc caaactgcta
     6841 ttcatcagcg tcacatcggc tgtttccatc gcaatgtccg ttccacttcc cattgccata
     6901 ccgacatcag ctaatgctaa ggccggtgca tcattgattc catcgcctgc cataccgact
     6961 tttttgccat ctttctgaag tttttctacg taggctgctt tatcctctgg aagtacttca
     7021 gcaaagatat gctttggatc aataccaact tgtttaccaa tcgtttcggc tgccagtttg
     7081 ttgtctcctg tcagcataaa aacatctaat cctttattct ggagctgctt gattgcttgt
     7141 ttgacttcta gtttgatttg atcagccacg gcaatcaacc cgatcacttg cttttcatca
     7201 gctaaaaaca tgactgtttt tccctgctgt tctaattcta acgcgtgttc ttggtattca
     7261 tcaaatgata ggttcaattc aattaatcgt ttacgtgtac ccgcaaaata gcgtactccg
     7321 ttaatcgtac cgctgattcc agcacctggg tgggctgtaa aatcaatcat atcgtatgtt
     7381 gctgcttctt gcagttttcc gtattccacg attgctttac ctagtggatg ttcagaagct
     7441 tgttctaaag tataaaaaat agataggact tctttagaac cgactagatc tgtaactttg
     7501 ggctttcctt ctgtaatcgt tccagtttta tccaaaacaa tgctgtctaa gtgagcagct
     7561 gcttctaatg cttctccgcc tttaattaaa attccatttc gtgcaccaac acccgttcct
     7621 accataatag ctgttggagt cgctaaacct aatgcacatg gacacgcaat gactaataca
     7681 gagacactat ggatcaatgc gagttgccaa tcaccagtga tcaaacctgt agctatgagc
     7741 gtgataaacg cgatgactaa tacgatcgga acaaaaatcc ctgagatccg atctgcgatt
     7801 ttttggattg gggctttgct cccttgagca tcttccacca tttgaatgat ttgcgctaat
     7861 actgtctgct tgccgacttg tgtaacttcc atgtgaagta gaccattcgt attgattgtc
     7921 ccgccaaaga gcgtgtcatc cggattcttt tctacaggaa gattttcacc agtaagcata
     7981 ctttcatcta tcgcactgct tcctgaaata attcgaccat ctgctggaat ctgctcacca
     8041 ggacggatga ctagttgatc accaacgact acttcttcaa tcgctaacgt ttcttctttc
     8101 ccgtttctta tcacttgggc ggtttttgtt tgaagagaca tcaattgttt gattgcatta
     8161 cctgttttcg tttttgctgt atgttccaaa tatttgccga gcaaaatcag cgtgatgatc
     8221 atactgctgc tttcaaaata aagatcttgg gggtgtccac ggaaaaatcc gttgtaaatg
     8281 ctcaatgcaa aggcagccga tgttccaatt gctaccaata catccatatt tggtgctttc
     8341 gtcttgattg catggtacgc acctttataa aaacgagcac cgacataaaa ttggacagga
     8401 gcagacagca ctaactggac gattggaaaa tgaaaaaagt gaacaatcgc cgcatggctt
     8461 cctaacatca tagcaatcat tgacaacatc aacggcaaag tcaaaaccgt actcaatata
     8521 agatcgaata gcattttttt caaatacgct tgcttttctt ctgcaatttt ttgtttgtgg
     8581 gcttcatcgt acaaaattgc accatagccg atgttttcta cgctttggat taatttttca
     8641 gtggtcgtcc cttcatattt gacacttgct ttttcagtgg ccaagttgac tgtggcattg
     8701 accacaccag gttgtccgtt cagctctttt tcgatcctag cagaacaatt tgcacaagtc
     8761 atacctgtaa tgacgaacgt atccattttg gtttctgctg ttgccatcaa atcacctctg
     8821 ctttataacc caatccattg attgcttgac aaatcttttc ggattgaact tttgtttcat
     8881 caaacttgac cgtaccgttt gcttttttca aatttacttt cactttttgc acgccgtcaa
     8941 gtgcgcttac tgtttcttct actcttgctg cacaatgatt gcagctcata ccttcgattg
     9001 aaaattgttg tttcattttt tcttcctcct attgatgttg tttacattca cattgacctg
     9061 ggatacagtt acattcgatc gtatctactg gttgcttttg ttccagttgt ttcaaaatct
     9121 cattgatatc ttcttgagtc aaagtggctt cttctactag atccgcaatc gtctctccaa
     9181 tcctttttgc acagatatgg gaaaaaagat tttccgtcgc actgcgaact gtttccatct
     9241 ccgagacagc tggataataa atgaattttt ttccttcttg ttctgtccag atcacttctt
     9301 tttttaccaa acgacccagc aaagttttga tcgttgctgg tttccaatcc atcgaatcag
     9361 ctaagatctg cgtgatttct tgggctgtcg tctgtccaag tgtccaaata acacgcatga
     9421 tctcccactc tgaatcactg atctttactg gcgttttgac tttttccatt tatttcacac
     9481 ctttcgttta caaatgtaat cattatctta tgaatacaga ttacacttgt aaacaaaaac
     9541 tgtcaattat ttttgctctc tattttcaat ttagttaaaa attcttgttt ctctcgtctt
     9601 gttttaagga tgatcagttt atttcttgca ttagctactt ggatcagctg ctgtattttc
     9661 ggttcattgt tttcttcaaa atgccagata aaagataaaa attctaagta ttccctgttt
     9721 agtttctccg aaaatgattc aggcatatct ggccgagatt ttttgtttat ctttcttttt
     9781 agagaacgcc aaactactcg gtagatggcg agataacgcg gcactttaag ccaaatgatc
     9841 ttatctgctt ctttaatcct ttcctctaat gtagaagtat aatttccttc aacgatccat
     9901 gacggattag ataccataaa caattgctgt tcttgcaaaa accacttttt tgcctgaata
     9961 ctgtagtctg ttgtatgcca taaaaggtct agcggcagta gcggaaagtc ggtgatttct
    10021 ttgatcttcc ttgcgagggt cgattttccc gacccacttt gtccaatcac gatgatcttc
    10081 attcttctcc ctcacttctc tttggaattt ggttcgatca taccacttat aacgccgcaa
    10141 aaaaacaagg gactgtgaca aaagtcgtgt caaagtccct tgtttcgaat aa
//
LOCUS       DACDMM010000093        10192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDMM010000093 DACDMM010000000
VERSION     DACDMM010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.094"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            119..625
                     /locus_tag="IWD16_001867"
     CDS             119..625
                     /locus_tag="IWD16_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP9088880.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="IWD16_001868"
     CDS             642..1541
                     /locus_tag="IWD16_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP9088881.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="IWD16_001869"
     CDS             1628..2512
                     /locus_tag="IWD16_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9088882.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="IWD16_001870"
     tRNA            complement(2704..2775)
                     /locus_tag="IWD16_001870"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="IWD16_001871"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="IWD16_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP9088883.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4372..5409
                     /locus_tag="IWD16_001872"
     CDS             4372..5409
                     /locus_tag="IWD16_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP9088884.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5450..6451
                     /gene="gap"
                     /locus_tag="IWD16_001873"
     CDS             5450..6451
                     /gene="gap"
                     /locus_tag="IWD16_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9088885.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6561..7751
                     /locus_tag="IWD16_001874"
     CDS             6561..7751
                     /locus_tag="IWD16_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP9088886.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7855..8610
                     /locus_tag="IWD16_001875"
     CDS             7855..8610
                     /locus_tag="IWD16_001875"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP9088887.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8726..10024
                     /gene="eno"
                     /locus_tag="IWD16_001876"
     CDS             8726..10024
                     /gene="eno"
                     /locus_tag="IWD16_001876"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP9088888.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2629 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
     4261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
     4321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
     4381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
     4441 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
     4501 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
     4561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
     4621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
     4681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
     4741 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
     4801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
     4861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
     4921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
     4981 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
     5041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
     5101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
     5161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
     5221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
     5281 atggcaatcg cgggcggaaa atcaaaagcc aaagcaattc gagcttatat gaaaaatgca
     5341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
     5401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
     5461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
     5521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
     5581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
     5641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
     5701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
     5761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
     5821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
     5881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
     5941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
     6001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
     6061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt
     6121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
     6181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
     6241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
     6301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
     6361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
     6421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
     6481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
     6541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
     6601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
     6661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
     6721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
     6781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
     6841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
     6901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
     6961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
     7021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
     7081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
     7141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
     7201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
     7261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
     7321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
     7381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
     7441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
     7501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
     7561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
     7621 actcagccgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
     7681 gtggcggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
     7741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg
     7801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
     7861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
     7921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
     7981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
     8041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
     8101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
     8161 catgaaacag acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca
     8221 ccaatcctat gctgtggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
     8281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg
     8341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
     8401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
     8461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
     8521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
     8581 ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
     8641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
     8701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
     8761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
     8821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
     8881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
     8941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
     9001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
     9061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
     9121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
     9181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
     9241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
     9301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
     9361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
     9421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
     9481 aaaaggtgtt tacgtactag ctgattcagg cgaaggcgaa aaaacaactg acgaaatgat
     9541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag acggattaga
     9601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
     9661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
     9721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
     9781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
     9841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac
     9901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
     9961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    10021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    10081 ttagttaagt aatcgaactc tctacttgct gaaatatcgg ctctttgtca ataaggactg
    10141 atgagttgtg caaaatcaaa tctgagtcag aatgaaccac attctggctc ag
//
LOCUS       DACDMM010000094        10104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDMM010000094 DACDMM010000000
VERSION     DACDMM010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.095"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..776)
                     /locus_tag="IWD16_001877"
     CDS             complement(<1..776)
                     /locus_tag="IWD16_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9088889.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLL"
     gene            922..1725
                     /locus_tag="IWD16_001878"
     CDS             922..1725
                     /locus_tag="IWD16_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP9088890.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1903..2850
                     /locus_tag="IWD16_001879"
     CDS             1903..2850
                     /locus_tag="IWD16_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9088891.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2918..3211)
                     /locus_tag="IWD16_001880"
     CDS             complement(2918..3211)
                     /locus_tag="IWD16_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP9088892.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3239..3904)
                     /locus_tag="IWD16_001881"
     CDS             complement(3239..3904)
                     /locus_tag="IWD16_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP9088893.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4044..4676)
                     /locus_tag="IWD16_001882"
     CDS             complement(4044..4676)
                     /locus_tag="IWD16_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9088894.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4683..5750)
                     /locus_tag="IWD16_001883"
     CDS             complement(4683..5750)
                     /locus_tag="IWD16_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9088895.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(5747..6478)
                     /locus_tag="IWD16_001884"
     CDS             complement(5747..6478)
                     /locus_tag="IWD16_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP9088896.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(6647..7126)
                     /gene="greA"
                     /locus_tag="IWD16_001885"
     CDS             complement(6647..7126)
                     /gene="greA"
                     /locus_tag="IWD16_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP9088897.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7262..8437)
                     /gene="mltG"
                     /locus_tag="IWD16_001886"
     CDS             complement(7262..8437)
                     /gene="mltG"
                     /locus_tag="IWD16_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP9088898.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            8641..9810
                     /locus_tag="IWD16_001887"
     CDS             8641..9810
                     /locus_tag="IWD16_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9088899.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         3095 a         2036 c         1576 g         3397 t
ORIGIN      
        1 cttagtagct gatcttcaaa atcaatatgt ccgtcgaaaa taattatctt ttcgctatac
       61 tgccaaatat ggacatgatg aacatccttt accccatcga ttgcttcaat ttcttgttcc
      121 attttttcat agtcgatagc aggtgcactt tgtatcaaaa tagcggcagc ttttttgatc
      181 accattatcg cttctatcaa tatatatatc gaaatgatca gtgtaataac tggatcgatc
      241 cagtagatat cgaaaaaacg gatcaaaatt gctccgataa tcacacttac cgaagacagt
      301 gtatcactta acaaatgcaa ataactagat tttatattca aactttcatc tgcttcttgt
      361 cgtaataata atactgagaa caaattcgcg agcaatccaa taactgctac tgttagcatc
      421 aaatgactat ttatcttttc tggtgaagtg aaccgcttga atgcttcaac ggttaaaaac
      481 aaggataata tgatcaatat gaatgagttg atgaaagcac tcaatatttc taaacgctgg
      541 tagccatacg tacgtttttt atttgcttct tttcgggcca ttttttgtgc cccgtaagaa
      601 aaaataattg ccaatgtatc actaaggtta tggaaagcat ctgaaacaag agacaaactc
      661 cctgacataa tccctccaat aaattctgat aaagtgatca ccgcattcaa aaccactact
      721 ccaaatagtt tttttgatga tctctgtggc tgtgtaggca ctattccttc ctccatttta
      781 tttatattgg atagtcttta gacaagaata acattttttt gatatttcac caatcattca
      841 gctgtttcct taaagcactt gtatttcact tgatacttcg ctagaattgc aagtgatgac
      901 actactagat gaggagtact tatgacaaca aaacaattaa ttatcagttc ctttatacga
      961 ttcaaagacc taaaggaggc gagaaatgtc ccattttgga aagccattgt ctatctttta
     1021 gttttgagta taattatggc tttgccaatc tcttttcaag tttttcaagt cttaaacaac
     1081 attcaattgg atgggcaaaa gattgctact cgcattcctg attttacaat ccaaaacggg
     1141 cagcttcaaa ccgaagaaaa agaaggattc atttaccaaa caaactccat tatcttcaca
     1201 tttgatccag agggaaaacg aacagaacag gacatctcta ctgatttgat ggggaatttt
     1261 gttagtgtcg ggatgttaaa aaacaaactt atcatagctc ttcctaatac aggcacgact
     1321 tctgctttgt tgaacaataa ccaattagaa cttccctaca ccaatgaatc tttaaaaaac
     1381 ttaacaggta agcagctccg ttctttttta agtgaagcat ctataccact ttggataaaa
     1441 gtcctgacat ttatattttc tatttatcct agttttctga atctgctgat cactttgcta
     1501 tttaccaaca ttgctgcttt tctctatgca cgctttcgac taacaaaagc tacttttctt
     1561 gattgtttaa aaacattgat ctattcaatt tcattgccta ctataattgc aacaatcttg
     1621 atggtctttc ttccttcttt tgacactagc gccttcattg cttttgcagg aatctttatt
     1681 tttgctcaag ctgtcaaagg atgggcaaaa atatcgatca attaagaaat aaagacaact
     1741 cgttatttct acttcaatag acccttttag aggttgtgac atttttttgt cacagcccct
     1801 tttttatttg aaaaatattt ctttctcaaa aaacaagcaa ggcgcttcaa agttcttctt
     1861 gtatgcgcta cacttagagt aaagaaaggc gggaaattaa ctatgtcaga aagaatcatt
     1921 tatgcagatc gtccatttcg tgatgaattt atcgaagaaa tccatactat agcgcctggt
     1981 tatatcttta aaacagaatt acaaccagag gatctacaaa atgtagagat cagtttgggc
     2041 tggaactcag actaccaaga aaaactatta gcctcttcta ccttgaaatg ggtccaatct
     2101 atctcagctg gtgttgatca gctacccctt gagacttttt caaagaaaca tattttactt
     2161 tcaaatggta gtggcatcca cagtgaatcg attacggagc atgtcttagg aatcatcctt
     2221 gggtattctc gtggtctttt tcaagcacaa aaggcacaga gtgacatgaa atggttaggt
     2281 tcatcggtcc attaccaatc tatccaaggg aaaaaaatac taatcattgg aactggccat
     2341 attggaaaaa tgattgcaaa aaaatgcaaa gcattagaca tgaactgcta tggaatcaat
     2401 acatccggtc atccagcaga aggaatggaa aagacttttt ctttaaatca actaaaggaa
     2461 gttcttcctg aagtagattt cgtagtcaat atcttacctc taacggagga aacaactggg
     2521 ctctttgatc agcatatgtt tgaacacttt gatccaaaat ctgtattcat caatgttggc
     2581 cgtggtgctt ctgttaagac acaagatctg gtgcaggctt taaataacca gcaactttcc
     2641 tttgcagcat tagacgtttt tgaggaagag cctcttccac aagatcatcc tctttggaaa
     2701 atggacaatg tactgattac ttcccacatt gcaggtttga cacctgattt tcaaaagaaa
     2761 ttaatggcta tttttttaac caatctgaaa agctactttg ccacccatga gcttcaaacc
     2821 aatcaagtaa aattgtctgc aggatattaa tagctgaaaa caatattttc aaaagaaaaa
     2881 agagaaggtg acaaaaattg tgtcaccctc tcttttttta ttgtttttta aagtgatagg
     2941 ttggctcctc caggttggga tcataatcaa caagtaaatc atatccttta aagaaccatt
     3001 catctgcttc ctcgataaaa aaaagtgtcc catccatttc cactttcttt acaggatgtt
     3061 ctggctgttc aactgacatt ccaatcgaaa aaccatcatg aacctctgtt ttgccgtaga
     3121 cttttccaaa aaaacgtatt ccgtaagttt ctggcagatc aatttctgag tcaaaccact
     3181 tttgtgcttt tggcgttacc gttaatttca tcccaacaac ctactttctc ttttctttct
     3241 attgattcat cttatcgtct agaatatcca catcgcctgt ctctccaata accagtagat
     3301 tatcattttc acgaacatat tcatctgctg caggagaagc gataaccgtt tgatctactc
     3361 ttcgtatggc gactactgtt aagtcaaaac gttgcctgaa atttaattca gctaatgttt
     3421 tgttgaaaaa tttaggattg gagactcgga tttctgctaa agaatattta tctgacaact
     3481 ctaggtaatc taaaatattc tttgacacta aattatgcgc aatccttatt cccatgtctc
     3541 gctcgggatg gacgacgcga tcagctccga tcttttcaag tactctggca tgatactcat
     3601 tttgggcttt tgctaaaata tttggtacgc ccatttcctt taccatcaga gtaactaaga
     3661 tactggcttg aatatcttct ccaatagcga caattacatg atcgaaattt cttatgccta
     3721 atgatcgaag tgtcatttca tcctgtgcat ttgccacaac agcatgagtg gctatattca
     3781 tatattcatt gacccgatct tcattactat caatagccag tacttcttga ccagcttcga
     3841 tcagtgttcg gcagatactt ccgccaaatc gtccaagacc tacaattgca aaattttgtt
     3901 tcaccgtttt cctcctttag tatcttcttt tctcagtata acaaagcctt tacaaaaaca
     3961 aaagcaagca tcattattgt aaaaaagcca agaaaaataa tagttatttt ctcagctttt
     4021 gattatcctc tttatcatcg tttttatttc accaaaccat gtttgaatgc atagatagcg
     4081 gcttgtgtac ggtcttccac ttctagctta gccaatatat tggaaacatg tgtttttact
     4141 gttttcagtg taataaacaa ttcgtccgct atttcctgat tactttttcc ttcagaaatc
     4201 agcattaaaa tttcattttc ccgattcgtt agttcttcat gtaatacagg ctcatttcgc
     4261 cgactcattt tttccatcat ttttgttgtc acttctggtt ctaatactcg ctcgccacgt
     4321 tgcgttgccc ttatagcatc agcaatttca tgtgcggtag aggtcttcaa caaataccct
     4381 gctgcacctg cttcaatggc aggataaact ttttcgtcat caataaaact ggttacgata
     4441 atgattttag cttctggcca atcttttaag attgcttttg ttgattcgat accatccatt
     4501 tcttccatga ctaagtccat taaaatgacg tccggtcgaa gtgtcatcgc cttctcatag
     4561 ccttgtcgtc cattttcagc ttctccaatt acttcaatgt cttcttgaat agataaataa
     4621 gaagagacgc ctaagcgcac catttcatgg tcatctacta ataaaacttt tatcatcctt
     4681 atctatgcct ccttcatcaa agggaccttg atttctacac ttgtaccttg acctttaaaa
     4741 ctaatgattt ttactgttcc accaatacca gctactcgtt ctttgatatt gttcaatcca
     4801 taacttcccg ttttcgtttc attcatatta aaccctgttc cgtcgtcaat aatacgtaac
     4861 aaaagattat tgtctatttt gtgcaaatat acctctaatt cattggcttt agcatgtctt
     4921 aatgtatttg ataacaattc ttgaacgatt cggaacaagt gatcctcaat ggaactagat
     4981 agttttacat cttctacatc ccatttcagc gaaatctgga ttttattttg caattccttc
     5041 aataactgct cgataccttg ctttaaactt ttctcttcta aattgactgg acgtagatga
     5101 agaagcagcg cacgcatctc agactgggaa gcgttgatga tctctgctac cattttcaac
     5161 tgcttgcgaa acatttctgg tgtctcgctt ttttctgctt gctccgtcaa tgccgacatc
     5221 atcatcattg ctgcaaacaa ttgttgcgaa actgaatcat gcagctcacg ggctaatcga
     5281 tgtctttcta attctaaaat ttcttctttg gtttgtccat ctacatattg gggacggctc
     5341 gttacaattt gtaattcact agatacttca atcatttttt cttttatctt agtgatttcc
     5401 ttatcaatat ctttgatgta taaatcgtcc gatgcacttg ggatcggaag aagcaacaat
     5461 ttggattcat aattgccaga agaaagtgca cgcagctttt cttcgatttt cccgtatttt
     5521 gtcttttgaa taagtgataa taatagaaag acaattaatc ctacacctaa ggaaatggct
     5581 aacaaatgga agataagcgg aacatataat aaacgtgcac gcagtaattc tccccaccaa
     5641 tgactttgat tgctggcata aaaataagtg aaaagtgaaa atagaataat aaggaaagcc
     5701 gcaatccccg aataaacagc tagcatcgct ctggatattt ttcccatcat attcgtatca
     5761 cctcgacatc tccaatcaag gtattagtaa taatcttcaa tctacgaggg ttttcatcat
     5821 aatcactgct gtagattttg atttgttcat ttttcaaaga aaattgctct tcttcaaaca
     5881 agacatttcc tactaatgtc gcatgctcta atcggatggc tacgcccaaa ggtactaaga
     5941 tccgtgttct gcctatccct tttcttacaa tcacaatatt atcatctttt ggcaaaagcg
     6001 tattgcccaa atctatgata gtatcaccag aaatgacggc gatgttgata tcgtcccatt
     6061 catagacgtc gtttccaata cgttggttac cgaataattg ctgtcttttc cgctcattat
     6121 tatgtgtttt taattgctct gtttggatca tcatgatctg tttctttcgc caaaaagcat
     6181 ttttggttaa atctatccct gaaatctcta cacctttcag accaataaat aaaacggcaa
     6241 atacagccat catccataaa gcaggactgt tgactaagct aaaaaaaatg accaaacttc
     6301 ctaatatcaa ttgaaaattt tgaaatttag tacgaggatg tttgcgcata gctagatata
     6361 tattgaaaat accgaatatc actaaaagca aaagtccagt attgtttacg atttgccaaa
     6421 cagcaaataa cagcagtagt gcttcaacta ccacaaaaaa acgccaagaa ctattcatcg
     6481 ctctttctcc ctcctgacac attctcattt catcatacct atttttttaa ctgttggcta
     6541 taggacttta gatgtaagat cgtttgttga aatctaaaat acacaaagag aaaaagatct
     6601 agagtgtatc atcagtaaga tgaactctct agatctttca gtcagactac gcttgttcta
     6661 ctttgacgat tttaactaac atatcgccac ctggagtagc aatagttact tcatcatttt
     6721 tacgtttgcc aatcaatgct tgcgcaatag gagaatcatt ggaaattttg ccagaaaacg
     6781 gatctgcttc agcgcttccg acaatcgtgt actcttcttc ttctccatct ggtagttcga
     6841 tgaatgtaac tgttttacca attgatacct catctgcatc tacaccgtcg ttatcgatga
     6901 tttgagcaaa acgaatcata ttttcaattg ttgtgattct tccttcaaca aatgcttgtt
     6961 catctttcgc tgattcatat tcagagtttt cggaaagatc tccgaaaccg cgggcaattt
     7021 tgattcgttc aatgatttct ccgcgtttga ctgtttttag ttcttctaat tctttttcta
     7081 atttttcttt accttctaga gtcattggat aaactttctc taccatgtgt aacgtctcct
     7141 tttttcgata aaataccgca aaaggtaatc ttttgaggaa tatagtccaa aaaagattac
     7201 cacgcttttc ttcaaatgta aatgtttttc taaaaatgat caatgtctag agcctgtttt
     7261 tctaagaatt atttacatat ttttccacta attccatatg ttcttcataa gttttagcga
     7321 aataaacatt tccagtgctc gtatctgcta cgaaataata atagtcattt tctgctggtt
     7381 ctaatactgc cttgattgcc tcttcgcttg gattgtcaaa aggtccaggt ccatagccgg
     7441 tattggtata caaattatat ggtgaatcta cagctgtatc ttcatacgtc actaattctt
     7501 tgtgttcacc taatgcgtat aaaacagaga tatctgactg aagtggcata tttgctttga
     7561 ttcggttaaa gaagacttgt gcaatgtttt tacgatcatt ttcttttacg ccttcttttt
     7621 caactagaga agctagagtt aaaacttctt gtacagtcaa atttttctgc ttgatagctg
     7681 aataatagtt ttgcatgacc gtattcgtct tgttcaccat ttcaatcact agatccttaa
     7741 gcgaagtact cgtgtaataa tcatatgtgg ctgggaataa gtaaccttcc aaacggtatt
     7801 ttgtgttctc agctttagat gcactttcca acagttctgg gaatttttgg tgcaactcgt
     7861 taaagaaagt ctcatcattc actaaatcta aaaattcttt cttgtcttta ccagttacct
     7921 tcgctacccg ttcagcaatc tgatcgatat cgtacccttc tggaatggca attttcccat
     7981 ctgcaacttt tgttggctca gaagtaccgc cttcttgcaa ctgtttgcca atctcgtcaa
     8041 gagtcatatt aggcgctaac tgataatagc cagcttgaaa gccagttaaa tttttaaatt
     8101 ttgtataata attgaaaaca atcccgcttt taataatatt gtccttctcc aaaatctcac
     8161 caatctgctt gttagaagag ccactcggga tctcgacttg gactaattgg tcatctgatt
     8221 tatctaaagg cttcaacccg ctatctacat aacgatacac ggtaaatcca agaaatccgc
     8281 caataatcaa taatgtaaga gcaatcacta aaatgatctt cctcacaatc ttatcttctt
     8341 ttttacgcag atttctccgc ttgtcgatta tatctgctgt agctgcctta ctggcttctt
     8401 ttttatcttt acttgggtgt tgtttgtttc ctgacaaaac aactcctcct tactatcaag
     8461 ttcgtaactt tattcattat acatgatatt tgaattggcg tgaatgattt tatttaaata
     8521 ttctatcaaa aatgtaagta gtaaatccat tactataaga ttaaaataaa tgttatcaag
     8581 atacaagcta ttttagcttt attctttctg taaaaaagaa aggagactaa ataaactatt
     8641 atgaaaaaag gaaaacatgc taaaaaaaga aaatattcat ttttaatgat cgttgtcgtg
     8701 ttgtttagcc ttagtgcgtt ctctacagga actattcttg gatggcaatt ttacaataat
     8761 gaacactctc agatagttgc ccatgctcaa tcagctcaaa aaaagttgat actgactacc
     8821 aatgaacaac agctcgttga aaaaatcgat caactattaa gaaaaaatga ttatattggc
     8881 tcaatttacg taaagaaaaa taatcgcatc atcatggaaa aaggctatgg ctatgcgaat
     8941 aaactcacgc aacaaccaaa tgatccttct ctttattatc agattggttc ggttcaaaaa
     9001 gcattgacta ctcttttgat tttaaaacaa gtcgagcgtg ggcacctttc tttagatact
     9061 aagctggctc aattctatcc acaaattgct ggcagccagc aaattactat ccgtgacttg
     9121 ctgtatatgc gctcaggctt gcaccgaact gcttctccaa ccataccgat gactgatgaa
     9181 gaagtcgttc aatttgcgat tcagcactta aaatttacag attatcagac ctctcattat
     9241 gattcattga actttaccat attgactgga attttgatca aattaacaaa tcaaccgtat
     9301 gaaaaattat tgaccgatga tatcatcgaa cctttaggat tacagcatac aaaattttat
     9361 gaccaagtca aaaattcatc caatcatgca ctatcctatc aaatgtctaa agaggatgat
     9421 tactttaaac cactgacaga atctgaaaca gagatccata acgaattagg gactggtaat
     9481 atcagtatgt ccgtctatga tttaaatacg ctgtttacac aagttttatc tggaaaacta
     9541 gtttccaaag acttactctt ctcattatgg cgtgaaaatg cgaatggaca tccatatagt
     9601 ggtggggtat atagcggaga taattatatc ctgtcgcaag gaaatatcga ctcttttgat
     9661 acatctgcta tatttagcaa agattcacag gatgctatca tcatggaaag caatataaaa
     9721 gctgacaaaa ctatcaaact gcctactgct gatctaagaa accaaatcta tgacctgatg
     9781 gaaggaacta ctaagttggc aaatagctga tatgatgaaa agatagcaaa tactacttct
     9841 ttgaatagca tttgcttaaa aaaagtactc gactaaacgt aggaaagagt gtgggacaaa
     9901 tctaaaaatc aatttttgct acagacttta attccaaata aacagaacag aaagaggtgt
     9961 tcttctttct gttctgtcca aattattcta agatttctct ttaccaacat tatttgacaa
    10021 agggcctatt aaatctaaaa tagtatcttt agattgctaa aatatgtgct agcaaatcat
    10081 tcatggcttg ttcttatttt taat
//
LOCUS       DACDMM010000095        10017 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDMM010000095 DACDMM010000000
VERSION     DACDMM010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.096"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(481..705)
                     /locus_tag="IWD16_001888"
     CDS             complement(481..705)
                     /locus_tag="IWD16_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088900.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            905..3673
                     /locus_tag="IWD16_001889"
     CDS             905..3673
                     /locus_tag="IWD16_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9088901.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            3754..4173
                     /locus_tag="IWD16_001890"
     CDS             3754..4173
                     /locus_tag="IWD16_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP9088902.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4185..4655
                     /locus_tag="IWD16_001891"
     CDS             4185..4655
                     /locus_tag="IWD16_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9088903.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4672..5451
                     /locus_tag="IWD16_001892"
     CDS             4672..5451
                     /locus_tag="IWD16_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9088904.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5438..6259
                     /locus_tag="IWD16_001893"
     CDS             5438..6259
                     /locus_tag="IWD16_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9088905.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6276..7325
                     /locus_tag="IWD16_001894"
     CDS             6276..7325
                     /locus_tag="IWD16_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9088906.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7338..8342
                     /locus_tag="IWD16_001895"
     CDS             7338..8342
                     /locus_tag="IWD16_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9088907.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8975..9994
                     /locus_tag="IWD16_001896"
     CDS             8975..9994
                     /locus_tag="IWD16_001896"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9088908.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3360 a         1651 c         2094 g         2912 t
ORIGIN      
        1 tgttcttgca atcttttttg ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta
       61 attgattttc aaagaaggag tatgaaaatg agaaaaatat ttattcgttt attgtgggta
      121 ctatcttaga ggaataaata atagtggaga aaactatggt ttggcgcaga ttctatcagg
      181 tttgatggaa cgaagacaac ctcccgacat caagtcaaaa gctacttgag attttgatct
      241 gaaaataaaa aaagaaaaca aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca
      301 atgggaaaaa actaagagtg gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct
      361 ctttactctc tgaaagcatc ataactcgtt tttcctcatt atgccaataa attgttgtta
      421 taccacatat aaggatttgt aatagctaaa aacagacagg aaagttgaaa tctgtgaatc
      481 ttattttgta tggaaggatt tatagaacgc aaaaatcaag ataaagatga ataaaaatcc
      541 tgtttcggct atatattgcg tatctacgcc aataaaacga gtttgttctg tcatagccat
      601 ataaagcaaa ggcaaaaata caactacagc gagataagat ccgatgattt ctcgaaaagt
      661 tttccaggaa gtatgtttcc tgtttttata tttatccgat ttcattgaat agtcatagcc
      721 tccttattct ttcatatgtt gaaattccag tataaaaact ggaaggaact gtacgttttt
      781 tagaataaaa attataaggg ataagtaggc tgctgtctat aataagatat atttttcaat
      841 tctttattaa agattttatt gacaaaatga atccgttttc tatgatggag gaggggtgaa
      901 taagttgttg aagaaggaaa ttttatgtta cttagagaac cagacagcct ttttcgattt
      961 agaacatatg aacgaggtct ttacagcaca aaggttagct gatcattttg gggtaaaaag
     1021 aaacacagtc agtcattatt tgaaccagtt aaatgatgag caactactag tcaaaatcaa
     1081 ttctcgacca gtgatttatt tccacaaaga agcttttgaa aaacaatttt ttaagttacg
     1141 cacgaatttt tatttttcta tcgatgatct caaagaggaa cagccttttt tcgcgcaacc
     1201 aaaagactta ttctctttta tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca
     1261 aatcaaaaca gctctttact atccagatgg cggattacca cttttgatta caggcgaaag
     1321 tggcacaggt aaaagttatt tagttaattt ggtctatcag tattgtctgc ttcatgatct
     1381 gctagaagat tctgcaccat tcatcacttt taattgtgca caatatgcag ataatcctga
     1441 acttttgact agcaatttgt ttggacatgt taagggagcg tacactggtg cagaagaaaa
     1501 taaaaaaggg gcttttgaag cagcagatgg tgggatcttg tttttagacg aggtacatcg
     1561 cttaacacca gaaggtcagg aaaaactgtt cacctatctt gatcaaggtg tgatttatcg
     1621 tatgggagaa cctaatttgt cacgccggat caaaacacgt ctcttttttg ctacgactga
     1681 agagattact agtcatttct taacaacttt cattcgaaga atacctatcc gaatcgaact
     1741 acctgcactt aatcagcgta gtcgtaatga acggctagaa ctgatttact ctttttttct
     1801 cgaagaacaa agaaaaatgg ggagcccttt gtcagtcagt ggaccagctt tgtcactttt
     1861 aacaagtcag aaattatccg gaaacatagg agagctgaaa aatatcgtca aagtttcagc
     1921 agcaaaagca tatgctgaac agcgtgaaca gcctgaaatc catgtcacga tccatcattt
     1981 gcaaaaagaa ctacttgcgc agcctattac taaaaataca gcacagcaag gaatctatat
     2041 tacgcaagac cagaccttag agcaattaat ggagaagcaa cagcccgaac agcagcgaat
     2101 cgttaaaagt tttgagcaaa tcctaattga tttcaagaaa aataactgtc tgctatattc
     2161 ttgcgaagga aaattaaagc aagaagtgat tcaattattt gattttttgc tgtttgagac
     2221 cagtagacaa gaaaaacacg agctgctggt ttattggaca caatctatcc gagagacctt
     2281 tcgacaaatg gaaactgcct atcaaatcaa attcgatgga aacagtgtct atgccttgag
     2341 ttactatctc tatcagcgaa gcactatcaa atggttaccg gaagaacatg atattatcca
     2401 actaatcaaa gaattagaac aacaagtttc tcagtcttat ccatctagtt atcactatgt
     2461 ccaacgaatt ctggaattaa gtaagatcaa attagatatt gaagacacaa gtatggatcg
     2521 gattttactg acgatctatc taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa
     2581 agcgatgatt gctgctcacg gttatgcaac tgctagcagt atggcaaatg tagtaaatcg
     2641 tttattacaa gaggatctat ttgaatcatt tgatatgcca ctagatgtca caccacagca
     2701 gattgcttca gaaatccttg attattgcga aaacagcgat gtttcaaatg gattgatcat
     2761 attagtagac atgggatctc tgaaagaaat ccatcagttt tttaaaaaac agttatccgt
     2821 accgcttttg attttgaata atgtgacgac acctttagca atcacagtag gggaatgcct
     2881 gcaaaaaaat gtatcattag aagagatagc agaagagact gttcgacaga tccagccaga
     2941 atggcgactg ctctatccag aggaaaacaa acccaaagca ctcattacta cttgcttcac
     3001 tggaatcggt acggcggtcc acttaagtga actgctagaa aaaagcttac ctacggcttg
     3061 tcagttgaaa atcatcccat atgaatatca gcaattaaaa gataagaaaa acagtgaccc
     3121 attattttcc atatacgaag tcgtaggaat gattggcacc acagatcctg atatcaccaa
     3181 tatcgactat ctttcattag aagacctcat ctcgggggaa aagatgtctg tactagctga
     3241 atggttggac actactatga atgcttcaga gaaggaaata ttcaatcagc gcatcattcg
     3301 taatttttca ttggaaaaag tactggattc cgtgactatt ctagatacag agaaggtgat
     3361 gggagaaatc gatcatttta tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa
     3421 tcccaaaaaa ttagcgttgt atgtccatgt gagctgtctc attgaacgat tgatccgaca
     3481 aatgccaatc gaaacctatc aaggattaga gaaatggaag cagtgtcaaa aagaagggtt
     3541 atctgctatt aaatctgcgt ttagtgtcat tgaagagcat tatagtgtca tgatccccaa
     3601 ttctgagatt gcctatatct atgatatttt gagtgaagat acagagtttc tctcgataga
     3661 agaagaattt tgaacataag caagttgttg ccactaattt tggcatgcaa cttgcttttt
     3721 tagtatgtgt aagtgaaaag aggagatgaa aaagtggata gagaaattat cttagcttcc
     3781 catggaaaat ttgcttcagg gatattagat tcattagagc tgatctatgg gaaaaatcat
     3841 tctattactg tattcgattg ctatactgat gagaattttg atttagcgga aacagtaagg
     3901 cagcttttta tgaggtacaa agataaagaa atcattgttc tgacggatct ttttggcgga
     3961 agtgtcaaca acgaattctt acaatatatc aatcgggatc atgtctatct cgttgccgga
     4021 ttgaatttgc cactattgat tgagctagta tctcggttgg acgctgaagt agagacaact
     4081 gttttgatcc agcaggtgct aactgattca aaaaaaatga tccagttttg taacgagcgg
     4141 ctcaatcaga aaatagaaga agacgaattt taggaggaag aaaaatgatt gtattgacaa
     4201 gagtcgacca tcggttatta cacggacaag tagctttttc atggacacaa acaataggcg
     4261 cagactgtat tttgattgct aatgatgatg tacctacgaa tgagatacga aagacaacga
     4321 tcaaattagc caagccacaa ggagtcaaac tagtgatcaa atcaattgat gactcgattg
     4381 ccgcgttgaa aagcggggtg acagataaat ataagttatt catcgtagtg gaatcgatcg
     4441 aggatgcata taagctggca gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga
     4501 tcaaggcaaa acctggtact cgcagcatcg gaaaagcggt aaatatttta ccagaagagg
     4561 aggaacgctt gaaacgatta attgacaaac aaatagaagt agaaatccgt caagtaccag
     4621 cagataaaaa agtggatgta agagaagttt tgtaagaaag gaagaacctc aatgattgta
     4681 caagcagtct tattaggaat tgttgcattt gtcgcacaat cagaatatgc actagggaca
     4741 tcgttgcttt ctcgtccgat tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta
     4801 aaaactggga tcattatggg cgcaacttta gagttagcat ttatcggatc gttctcagta
     4861 ggtgcttcga ttccacctga tgttgtgacc ggtgggattt tagggacggc ttttgctatt
     4921 acagctggag ccggtacaga aaccgcgctt ttactaggtt tgccgattgc tacgttgaca
     4981 ttgatattga aaaatattta cttagggctt ttgattccaa ttatgaacca taaagcagac
     5041 aattatgcag aagaaggaaa ttataaagga gtcgagcata tgcacttgct tgccggtatc
     5101 ggtctttcct tgatgcttgg tgttgtggta atggtttctt atatggtggg aagcaatacg
     5161 atcggaaatc tattaaatat gattcctgac ttcgttcaaa aggggttgtc agtagctaca
     5221 gggttgattc cagctcttgg ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt
     5281 gcaccatatt ttttcttagg ttttgctata gcttcttact tagagattcc tgtgaccgga
     5341 gtagctattt ttggtgcgat tttagcagtt gtggtagtca atattatgaa tgtccgtcaa
     5401 ctaccacttc aaacaactgc aggggaggta gaagaagatg aagaattctg agatcaaaaa
     5461 cagcaaaata acgaaaaaag agctaaatca cgtattttgg cgttcttttc aaatggagtt
     5521 ttcttggaat tatgaacgtc agatgaatat ggcttatgtt tatgcaatga ttccgatttt
     5581 gaaaaagtta tatcagtcga aagaagaaat ggcagcagct ttgaaacggc atctagagtt
     5641 ttttaacacg accccacata tcgttacact gattttagga atcacggctg caatggagga
     5701 agaaaataag gatgatcctg aatttgatgt gactgcgatc gatagtatca aaacatcttt
     5761 gatgggacct cttgcagggt taggagattc tttcttttgg ggaactttgc gattgattgc
     5821 cacaggtgtg ggtacctctt tagccatgca aggaaatatt ttaggcccta tcttgttcat
     5881 tttgattttc aatattccgc acattctgtt tcgctatcta gcaacaggtt ggggatatcg
     5941 cttaggaacg ggttttttga aaaagatcca ggcaaacggt atgatggaaa gtttgacatt
     6001 aggcgcgtct attattggac tgatggtcgt tggagcaatg actgccacga tgatcaccat
     6061 caatattcct ttaaaaatcg gttcaggaga aaatgctgtg actgttcaaa gtatttttga
     6121 tgatatcgtt ccaaatatct tatgtcttgg agcatttggc atagtgtttt atctattgaa
     6181 gaaagaagtg aaaccattaa cgatcttgat cggactagct ttctttggaa tttttggttc
     6241 gtggataggg attttttaaa aaaggagcag agaaaatgtt tacgatgcaa gattatatct
     6301 atgaagaaaa ggaagtacta agtacgatct tgaaaaagaa tgatttttcg acaagagaac
     6361 atatgaaaaa aacagtgaat ttactgatct tagcaacggg ttcatcttat aacgcttgtt
     6421 tagcagcgaa gccagcattg gaatcttatg gtgatcttac tgtcgatatt caagaacctt
     6481 tttatttttt caattatgga aaactttctc cttcaatcga tactgtgatt gctgtgtcac
     6541 aaagcgggaa aagtgcttct actgttgaag cagtaaaaat gatacaaaaa caagggttac
     6601 ctgttgttgc tatcacaaat gacgtgcaaa gtccacttgc tttagaagct gcacaaatta
     6661 ttgatttagg tgccggggta gaatcagtag gcttcgttac aaaaggctat tctgcgactg
     6721 ttttacagtt actactctta gggataggaa ttggaataag taaaaataaa atcagcaaaa
     6781 aaatagaaca agactacatg caacagttgc gcaaaataat caaccattta ccagcgatta
     6841 ttcaaaaaac ggaagaattt tttgatggat atcaaagttt atttcgttta gcacagcgtt
     6901 tcgtcaccat tggatatggt ccaaactggg gaacagctaa agaagcagag acgaaattga
     6961 ctgaaacaat ccgcgttccg tcacaaggat ttgagttaga agcttatatg cacggcccat
     7021 acctggaggc agatgcttcg catttgctat tctttattga aggggaaagt gtaaataagg
     7081 aacgttcgca aaagttgcaa agatatatga gtcggtacgt tggagagact ctcaccatta
     7141 ccacaaaaaa agcaagaaac gagaaaacac ttggattagc aatagaatgt gatgaatatc
     7201 tgtctgtatt agcgcttgtc gttccgtttc aactattcgc ttataaaacc gcagtagcta
     7261 aaggtattga cttgaacaaa aagatttttg aagactttga tacagtcttg aaaagtaaat
     7321 tataagaggt ggaaatcatg ctgaaattca atgaagaaga acaaatcaaa gcaataaaag
     7381 gcgcactagc tttacgtccg caagtagaag aaatcatcga taaactttat gcggaaaaat
     7441 ttgacgctgt ctattatcta ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca
     7501 cttatatgaa tgggaaaagc aacttgcctg tctttgtaca gcatgctgca gaatattaca
     7561 ctactggaaa caaaagatta acaaaagatt ctatcgttat tttgtcctct gttaccggta
     7621 caactcaaga agtagtgaaa gcagtagaac aaatcaagaa agtaggagcg acactgattg
     7681 gatttatcga taaagcgaac agtaagttgt ctcagctatg cgattttgtg gtcacttatc
     7741 cagctcctgg aacagaacaa atcaaatttt ttatggcagc agatcgattg atgtatttga
     7801 atggagaatt cgaagcttat tcagaatact atgaacagct cgaacgttat ttaccaacag
     7861 ggttagtgga agcagagaaa aaagcagatg catttggctt atcatttgca gaaaaacatc
     7921 gacgtgatag tatgcattat tttattggag cagggaatca atggggagct gtttattcat
     7981 atgccatgtg ttactgggaa gaacaaagct ggcttccatc gaaatctatc catgctgccg
     8041 agtttcttca cgggacgtta gagatcgtag aagaaacaac cccagtcaca ttatttttag
     8101 gcgaagatga acagagagtg ttatctgaac gtgtggctaa acttttgcca aaaatttgca
     8161 gcaattatac attgatagac acaaaagact atccagtaga aggtatcagt gaaaaatatc
     8221 gcggacgtgt tttgtctttc ttattgatgc atgtagtgac acaacgcatc gatgctcatg
     8281 tggagcagtt gaactgccat ccattagaga tacgccgcta ctatcgtcag tttgattatt
     8341 aggatagact ggataaaaag aatataaaaa ataaagagag cagcgataac tgctcccttt
     8401 atttttttaa aatattggca tcaattacgt tataaaacac attttttgca ttatctacag
     8461 ttcaacttat aatctgttta atgggacatg tgggtgccaa ccattttttg ttatgcaata
     8521 gtactattta gttgttgcat ggatccgtag taaaagtcca agtacaacga gttgcgccag
     8581 tttacatagc gttttgtatc cagaatgttg actaaaacaa aagttcctat gtcaaccgtc
     8641 aacagagaca atctgtccgt tgcaggacct gacagattta gaaatgttca atctgtctct
     8701 tatgtatttt aaatcaatac tcactaggag aagtttccta acctcctaat agtaatgctg
     8761 gtagtaacat tgtcaaaact gcttcctctt aggaacaagc aaagcataac gagtgtaaaa
     8821 ataaaatgta gactattgta acatatcatt gattatttca agtaaaaacg ataaaagaag
     8881 taaacaaaca cttaaaaaca atggaaatta gtcccaactt taagaaatgt atactataat
     8941 ggaaattatc aattatttat ttggaggaat atccatgaaa caaaaaatcg cagttcttgg
     9001 tcctggatct tggggcactg ctttagcaca aacattggca gaaaacgggc atgatgtccg
     9061 tatttgggga aatgtacctg aacagattga tgaaatcaat aagtatcata caaatcagca
     9121 ttttttacct gatttgacga tcccagaatc gattatcgga tataaagaat tggcagaagc
     9181 agtggataac gcagatgcta ttttatttgt tgttccaaca aaagcgattc gttcagtcgc
     9241 acaggaactt attggtaaaa tgaatactaa gccaatcatt atacatgcaa gtaaaggact
     9301 agaacaagat acgcataaac gaatctcaga agttttggct gaagaaattc cagaagaaaa
     9361 aagacaggca attgttgtat tatctggtcc tagccatgct gaggaagtgg cagtacatga
     9421 tatcacaaca attacagctg caagtattga tcaaaaagca gcagcctatg tacaaaaact
     9481 gttcatgaat gattatttca ggatttatac aaataatgat gtgattggcg tagaaactgg
     9541 tgcagcactg aaaaatatca ttgcaattgg tgccggcgct atccatggat taggattcgg
     9601 agatgatgca aaagcagcaa tcatgactcg aggattggca gaaatcagcc gtcttggtgt
     9661 cgcgatgggt gccaatcctc tgacatttat cggtttgagt ggtgtggggg acttgatcgt
     9721 tacttgtaca agtgttcatt ctcgcaattg gcgtgccggt aacttgttag gacagggaca
     9781 taagctggaa gaagttctag aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa
     9841 agcagcagta gagctagctc aacagctagg tgttgagatg ccaattactc aaacaatcta
     9901 taatgttttg tataatggag aagatatcaa aaaagcagca aaagaaatca tgttacgtga
     9961 cggaaaaatg gagaatgaat tttctcttcg ttaaatagat gatcaatagt taatcgt
//
LOCUS       DACDMM010000096         9999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDMM010000096 DACDMM010000000
VERSION     DACDMM010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.097"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(65..217)
                     /locus_tag="IWD16_001897"
     CDS             complement(65..217)
                     /locus_tag="IWD16_001897"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088909.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(408..1286)
                     /locus_tag="IWD16_001898"
     CDS             complement(408..1286)
                     /locus_tag="IWD16_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9088910.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1325..2443)
                     /locus_tag="IWD16_001899"
     CDS             complement(1325..2443)
                     /locus_tag="IWD16_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9088911.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2440..4539)
                     /locus_tag="IWD16_001900"
     CDS             complement(2440..4539)
                     /locus_tag="IWD16_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9088912.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4590..5411)
                     /locus_tag="IWD16_001901"
     CDS             complement(4590..5411)
                     /locus_tag="IWD16_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9088913.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5408..6217)
                     /locus_tag="IWD16_001902"
     CDS             complement(5408..6217)
                     /locus_tag="IWD16_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9088914.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6238..6732)
                     /locus_tag="IWD16_001903"
     CDS             complement(6238..6732)
                     /locus_tag="IWD16_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9088915.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6744..7232)
                     /locus_tag="IWD16_001904"
     CDS             complement(6744..7232)
                     /locus_tag="IWD16_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9088916.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7309..9927)
                     /locus_tag="IWD16_001905"
     CDS             complement(7309..9927)
                     /locus_tag="IWD16_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9088917.1"
                     /translation="MKKSAKDRVFDELIRLTGNMSSVTAEKISKNLGISRQNASHYLT
                     RLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQINEAFHSVVGHDGSLREVIQKCIAAV
                     KYPPNGLSVLINGATGVGKSFLATKIFEYAIHEQIIEKDAPFAILNCADYADNPELLS
                     ATLFGYKKGAFTGAEKDTDGLLATANNGYLFLDEVHRLSKENQEKLFLFIDTGNYRPV
                     GENVNWHSAKVRFIFATTEKGENYLLDTFDRRIQISVMLPTFEDRPIRERLELIQLFF
                     QNEADVLQKDIIVSREALSLMLSHPFSGNIGKLKNIIKISCADVYSRTQEEPLTIGKN
                     EILIQLNMLESEVESLPIASLLIKKNQKIKTDRIKPFDYDSILEKITQLINEGNFNEN
                     FSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMFRKVWVSVLGKTYGLTTSGPVAEIISR
                     SYVISPSYKTDLTGINRTLAANLPRTTYLCNQFLEHLPPLSEKDENFLRLLIVVTLSD
                     YVDESIELKGLLLAHGDSTASSIQAVVNQLCGNYVFEALDMPVESNVSEIVESTRNFV
                     KRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGELLLLNNLTTSIALDIGLKMTMNAPFK
                     QIAEDAADKYSINVQYFEGFSQSSNIIISCMSGLGISDKLKDVFRHHLSDESIEVFTK
                     DYRELRELIDQNDETYFDKTKLVITTSDLPRSFSIPNINVYDILDGDGAANLEHILSP
                     EMKPAEFNRLIQELVKFFSLEGIADRLNFLNPTIVMSEVETVISKYENYYHFTLNGRV
                     KLNLYMHTALMMERLFLARTEKSEEPNYDLKPDEDEFYYVSRSIFQSMEMKYNFKVNA
                     YELSLLYELLGPYIQK"
BASE COUNT         3037 a         2256 c         1650 g         3056 t
ORIGIN      
        1 ctttttataa tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa
       61 tctatcacca aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa
      121 tgaacataca ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat
      181 tactatttaa gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag
      241 aaaaaaggaa acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac
      301 ttagagcctt tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt
      361 actcggttaa atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta
      421 tttcttaccg cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat
      481 tcgggcgtaa taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca
      541 acaaatttat cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa
      601 ataattctat ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag
      661 gcttcaatct gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt
      721 ttttcaatcg ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt
      781 cctgaaaaat tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg
      841 aacccttcca cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg
      901 aagtatgcaa cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc
      961 gcattgacat ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca
     1021 aaattgaaat tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat
     1081 ttgcattgaa tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta
     1141 aaaggtttaa aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa
     1201 ctgactcttt ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct
     1261 ccagcttcaa ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac
     1321 ttcattaacg cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt
     1381 ctgttgaata ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat
     1441 catcgttgcc gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt
     1501 gaagatgcca acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg
     1561 cattcaattt tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat
     1621 accctaaaaa ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat
     1681 gaaaggccca cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact
     1741 cactatcccc atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc
     1801 aggtaagcgc aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact
     1861 tataatcctc ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc
     1921 tattttcttc caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat
     1981 ccttcggatg taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat
     2041 aggaattaaa gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct
     2101 ctccaaaccc attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc
     2161 catcccaata aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat
     2221 cacgaatcgt catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag
     2281 tttcttcacc agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc
     2341 ttggcaacat cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac
     2401 tctggatttt tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat
     2461 gtgaatcgtt tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg
     2521 cttttctgac aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc
     2581 ttgaccgaac ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa
     2641 tactcctttt ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc
     2701 cgatgcccag ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc
     2761 taagcgatac ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc
     2821 taatcgggtt acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc
     2881 aggaaaagtg gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt
     2941 tggattgaaa gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc
     3001 taaaaattga taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa
     3061 attaattttt ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg
     3121 tttttgatag tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa
     3181 ttgctcacca aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca
     3241 ggcaatgttt ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc
     3301 aagtccaata caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc
     3361 aaaggcgtct gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc
     3421 taatatgtcc tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa
     3481 ttttgtatcc ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt
     3541 actcagagaa tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg
     3601 atcaaaccat agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga
     3661 tgcgtaatac atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct
     3721 gccgacgacc tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg
     3781 acgatagaga tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa
     3841 atagttcaat gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc
     3901 ttcaaaagca tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat
     3961 cgtgtcctta tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg
     4021 atacgaaggc acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta
     4081 aacctgttga acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac
     4141 aatgacctta aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc
     4201 gacttcaata cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt
     4261 taaggataac gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc
     4321 agttagtaaa ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt
     4381 tgctaatgtc ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg
     4441 atagtagctg tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa
     4501 tgggtgtttg ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa
     4561 aactgcagcc tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt
     4621 cccaagacga ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg
     4681 aaacagctca atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct
     4741 tggacattca ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc
     4801 atactgatcg tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga
     4861 atttcaatta acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct
     4921 aaaacgccgc catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata
     4981 attggtgcca aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca
     5041 cgccaaactc cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt
     5101 ttgatcgcgt tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc
     5161 gatgccgcaa tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta
     5221 tgacgttcca gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc
     5281 atcatagaat aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata
     5341 gtcatcgagc gccaaaacat cttccgaacc aattttttct catctttaga aattccatca
     5401 gctaatttca ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat
     5461 cgctttgaac tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa
     5521 ttgcaataat cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa
     5581 caacggcccc aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca
     5641 tcgccatccc aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct
     5701 ctggaatatt atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg
     5761 gaataagagc ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc
     5821 ctttttgatc accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa
     5881 aagaaaaaat catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa
     5941 taatcgcatc cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca
     6001 aagaaggctc cgccgtacta gagattccga tattgacgac acccataaag atggcttcta
     6061 gtgacgcccc gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag
     6121 caacaacaat cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaattccaa
     6181 taaaaacggc caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta
     6241 aaaatcactc aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac
     6301 ggtatctgga taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat
     6361 cacttccttt gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc
     6421 cacgttcatt aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac
     6481 attagggact aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat
     6541 cgcgtcatca actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt
     6601 catcttttga atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt
     6661 tagactcttt gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac
     6721 tgctttaatc atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc
     6781 tcatcaagaa tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc
     6841 tcctttgtta attccttggt tgccaatact atttcgagca gaattggcag attaaagcca
     6901 gacaaaatag ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca
     6961 cttccgccaa ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca
     7021 aatacattca actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc
     7081 tcttgaacct tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg
     7141 tgagtcgcta ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata
     7201 taagcaattc ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa
     7261 aaaagtgtct aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat
     7321 atacggtcct aataattcat agagcagcga aagctcataa gcattgactt taaaattata
     7381 tttcatctcc attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg
     7441 tttcaaatca taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat
     7501 catcaatgcc gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata
     7561 gtaattttca tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag
     7621 aaagttcaaa cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa
     7681 gcggttaaat tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc
     7741 atcaccgtct aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc
     7801 cgaagtcgtg atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa
     7861 ttcacgtagt tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg
     7921 acgaaaaaca tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat
     7981 gttgctactt tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc
     8041 atcctccgca atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc
     8101 aatggaagtc gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg
     8161 tgtgtaaatt tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac
     8221 ttgccgtttc acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg
     8281 catatccaac gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct
     8341 gctagccgta ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac
     8401 ataatcagat aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag
     8461 cggtggtaaa tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc
     8521 taatgtacga tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct
     8581 gctaataatt tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga
     8641 tacccagacc tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc
     8701 cttatcaatg tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt
     8761 gaaattacct tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg
     8821 tttgattcga tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa
     8881 gctttctact tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt
     8941 taatggttct tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt
     9001 cagcttacca atattcccac taaagggatg agataacatt aaagacaagg cttctcttga
     9061 aacaataata tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc
     9121 taatcgttct cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg
     9181 ccgatcaaag gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg
     9241 aaccttagca gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat
     9301 aaatagaaag agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag
     9361 atagccgttg ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc
     9421 ccccttcttg tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc
     9481 acagtttaag atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc
     9541 aaaaattttt gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact
     9601 gagtccgtta ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact
     9661 tccgtcatga ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac
     9721 cgcatattca tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt
     9781 atcttccact aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt
     9841 cgaaattttc tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa
     9901 aactcgatct ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata
     9961 ataaactatc ggctgcaata tgtctattct atatttgta
//
LOCUS       DACDMM010000097         9983 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDMM010000097 DACDMM010000000
VERSION     DACDMM010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.098"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            206..475
                     /gene="rpsN"
                     /locus_tag="IWD16_001906"
     CDS             206..475
                     /gene="rpsN"
                     /locus_tag="IWD16_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9088918.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            490..669
                     /gene="rpmF"
                     /locus_tag="IWD16_001907"
     CDS             490..669
                     /gene="rpmF"
                     /locus_tag="IWD16_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9088919.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            701..1078
                     /locus_tag="IWD16_001908"
     CDS             701..1078
                     /locus_tag="IWD16_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088920.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1099..1248
                     /gene="rpmG"
                     /locus_tag="IWD16_001909"
     CDS             1099..1248
                     /gene="rpmG"
                     /locus_tag="IWD16_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9088921.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1272..2255
                     /locus_tag="IWD16_001910"
     CDS             1272..2255
                     /locus_tag="IWD16_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9088922.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2285..2407
                     /locus_tag="IWD16_001911"
     CDS             2285..2407
                     /locus_tag="IWD16_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9088923.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2425..3948
                     /locus_tag="IWD16_001912"
     CDS             2425..3948
                     /locus_tag="IWD16_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9088924.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            3972..4211
                     /locus_tag="IWD16_001913"
     CDS             3972..4211
                     /locus_tag="IWD16_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088925.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4404..4865)
                     /locus_tag="IWD16_001914"
     CDS             complement(4404..4865)
                     /locus_tag="IWD16_001914"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9088926.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5060..5278)
                     /locus_tag="IWD16_001915"
     CDS             complement(5060..5278)
                     /locus_tag="IWD16_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9088927.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5396..7012
                     /locus_tag="IWD16_001916"
     CDS             5396..7012
                     /locus_tag="IWD16_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9088928.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7253..8593)
                     /locus_tag="IWD16_001917"
                     /pseudo
     CDS             complement(7253..8593)
                     /locus_tag="IWD16_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(8869..9024)
                     /locus_tag="IWD16_001918"
     CDS             complement(8869..9024)
                     /locus_tag="IWD16_001918"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9088929.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(9320..9652)
                     /locus_tag="IWD16_001919"
     CDS             complement(9320..9652)
                     /locus_tag="IWD16_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9088930.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
BASE COUNT         3311 a         1593 c         1981 g         3098 t
ORIGIN      
        1 agcctaaact gcatattctt ttaaatgatt tgattatctc tatgtaccga taaattcgtt
       61 ttcaaatagt tatctattca tatgagtttt atttcatgtt atttattgtt gacaagagca
      121 actcttgttg gtaaagtatc agtgtcaaaa cgtaacgatt acgatttgag gtgtctaaat
      181 caaaataaaa ggagtataaa ggtatatggc aaaaaagtcg aaaattgcta aagcaaagaa
      241 acaaatggcg atgattgaaa agtatgcaga taaacgtcag gaattaaaag ccgcaggaga
      301 tcgcaccgct ctcgctaaat tgccaagaga ttcaaatccg aatcgtctga ggcttcgtga
      361 tcagacggat ggtcgtccaa gagggtatat gcgaaagttc ggtatgtccc gtattaagtt
      421 tagagaactt gcccatcaag ggttgattcc tggagtaaaa aaagcaagtt ggtaaaagga
      481 ggaaatatca tggcagtacc agcaagaaaa acatctaagg cgaagaaacg gttaagaaga
      541 actcatcaaa ctgtagtaaa accagaaatt tcatttgacg aagcgaatgg tgattataga
      601 cgtagccatc atgtatcttt aaaagggtac tataatggaa agaaagtaat taaaggagct
      661 tcaaaatagt ttaatatata acttttagga gggaatcaaa atggaaatta tttacccacc
      721 tttagttgaa gaaggattaa agtattatct tgaaacgacc cagcaatcgc tagataaatg
      781 cactttttat cgttcgatgg tagagagagg aattattact gaaacgggtt taccgaccca
      841 acaggcaatt gaaaatggat tagtcaaaga ttactatgag gaccaaggct tatcatttga
      901 tgatttttta agaatctatc caatttttga agagtatgac gaggagttat ttcaatgtat
      961 cgatggttat tgggagatac ctgttgatat gaaagaaaat ttagtttcac aattggaatc
     1021 tggcgagtta acttttgaag atgcacaaca aattcaagca tatcttgaag atcgataagt
     1081 attgaaggag gaagatttat gcgtcaaaat attattttgg aatgtgtcga aactggagaa
     1141 cgtctgtatc tcaccagcaa aaataaacgg aataaccctg aacggttaga attaaaaaag
     1201 tattcgccaa aattgcgcag acgggcaatt tttaaagaag tgaaatagct aagtggtaaa
     1261 aaggtgacaa aatgggcata ccagttacaa tcgtttctgg gtttttaggg gctggaaaaa
     1321 caacgctaat caatcaagcc ttgcaagtct caccctaccc cagggaagaa attattataa
     1381 ttgaaaatga gtttggtgaa gttgggattg atcacaaatt attagttcat agcgaggaac
     1441 gggtgttaca actaaataat ggatgtatgt gttgcagtct tcgcagtgat ttattagcaa
     1501 tattgatttc tttactggaa acaatagaag aacatcaaca accaatttca caaatcatca
     1561 ttgaaactac aggtatagca gatccccaac caatcattca aacactttta acagcgcctc
     1621 aactaagagg aagagtatat atcgatagtt tattgactgt tattgatagt gacagttttg
     1681 aacttattga aagagaacca caagccctta agcaacttgt catggcagat cgcatttttg
     1741 tgtctagaaa acaaaacttg aatttatctg cactaaaaaa attacagaga gaaatcaaaa
     1801 gtattaaccc tttatccgat attcgaatgt tcacacatag tgaaacgatt caaaaaaagg
     1861 atttttataa tttgaaaaaa tttaatcatc ccttagtttt tgaaagtgat tcatcagaaa
     1921 atcactataa tggcaaatac tatcatcaac accatcaagt acatgagttt aaatcaattc
     1981 tcttaacttc agatcaagac ataaaaaaag attttttact ccaatggatt gactggctga
     2041 ttttaaccaa ccaagggagc ctgtatcgtt ttaaaggatt gattgcttta caagagcagg
     2101 attttatagt tgccatgcaa gaggtaaacg aacaggttaa ttttcaatac accactcgac
     2161 aaaaagacca aaccactatt attctaatcg gaaaaatgtt ggatgaagaa aggatcaaac
     2221 agagttttga taaattactg gcggaaagca aataatgata agtagtgatt gggggtaatt
     2281 tctaatggag aaaacggata tatctagtgc atatcgacga cttaaaagcc cgaatattaa
     2341 aactagaaaa agggcattaa aaattatcaa agaacataaa cgaaataaaa tgaagaaatt
     2401 agcataatta aggaaggaaa aagaatgaag aaaatcagtg taggacttat tgggatagca
     2461 gctctaggat tattgggagc ttgctccagc actaatgatg caaaagtatc gaatgataaa
     2521 gatggaaaat tagaaattgt aacaacattc tatccaatgt atgatttcac caaaaacatc
     2581 gtcggcgatg aagcgaacgt ggatttaatg gttccagcgg gatcagaacc tcatgactat
     2641 gagccctcag ctaaggatat ggcaaaagct catgatgctg atgtttttgt atatcataac
     2701 gaaaacatgg aatcatgggt accaaaagct aaagaaagtt ggaagaaagc tgggcctaat
     2761 gtagtagaag gaacaaaaga catgatttta ttgccaggta gtgaagaaga agatcatgat
     2821 cacggagaag aagatcacca tcatgaacta gatcctcata cgtgggtttc acccaaaatg
     2881 gcaattaagg aagtttcaaa tatcaaggat cagttagtta aattgtatcc gaaaaaagca
     2941 aaagtatttg agacaaatgc tgaaaagtat ttgactaaac tgaaacggtt ggatgctgac
     3001 tataccacta gtcttaaaga agcgaaacaa aaaagttttg tgacgcaaca cgcagccttt
     3061 ggatatttag ctttggatta tggattgaaa caagttccta ttgctggact aagtcctgaa
     3121 gaagagccat cttctggtcg attagctgaa ttaaaagagt atgttaaaaa aaataagatc
     3181 aattatattt actttgaaaa gaatgctaat gataaaattg caaaaacttt ggccaacgaa
     3241 gctgggataa aattggaagt actgaatcct cttgaaagtc ttacaaaaga acaaatggat
     3301 aatggtgaag attatgtttc agtaatggaa gacaatctaa aagcgttaga aaaaacaact
     3361 atggttgctg gtgaagaggt agttccagaa aaagaagcga aggatgaaaa gacagttgct
     3421 aatgggtact ttaaagatgc cgatgtgaag gatcgcgaat tgagtgatta tacaggcgaa
     3481 tggcaatctg tttatccgtt gttaaaagat ggaattttgg atgaagtttt cgattacaaa
     3541 gcaaaaatca ataaagacat gacagctgcc gagtacaaag attattatac tactggctat
     3601 aagacagata ttgatactat caacattaaa gacaacacca ttgattttgt agtcaacggt
     3661 gaacatcatc aatacacata caaatatgtt ggatacaaaa ttttgaatta tgaaaaagga
     3721 aaccgtggtg ttcgttttaa ttttgaaact gatgatgcag gtgctggtcg ttttaaatat
     3781 attcaattta gtgatcatgg aattgcacca agtaaggcag agcatttcca tattttcttt
     3841 ggtggagaaa gtcaagaaaa actctataat gaattgcata attggcctac tttctatcca
     3901 gcttcattgt ctaaacatga aattgctcaa gaaatgatgg ctcattaaaa ttcaattttt
     3961 ggaggaaata catggtaaga aaagtattat tgtctatgga ttccaagaat attgatgctt
     4021 atcggcgtta tcgatttgca aagcaaatga gattaaatgg gagtacatta gctgatattg
     4081 ctgcaacttt acatgttagt attgacacag ttcatagagt attaaaacgg gatcctgatt
     4141 gttgttttat caaggtcaat actggaagaa gtagacgttt agcaaaacgg cataatagct
     4201 tggccaatta attgataata ccagagtaga tcatttttcc cttatttgga tttgatctgc
     4261 tttttattat gtcttgaatt gtcaaatcaa gcgcaacact ttatcaaaga agacttcata
     4321 aggtgttctc caatctaaac actttctagg tctgttattc agtttttcaa tcatctgatt
     4381 tatcgttgag ttatctaccg tagctatctc tttcgatttt ggcaagtatt ctcgaatgag
     4441 tctattcgta ttttaatttg ttcattgttg ccaagggcat gaggatctgg aaaataaaag
     4501 ggcatattat tcaatgcttc tgtaactgcg aaatgattgg agaattcttt tcctctatct
     4561 ggagtgatcg ttttgaccgc ctcatttgga atactgctta aaagttcaat cattttctcg
     4621 ctcacataag cggctttctt tctggggacc ttctttcctg ctaaaaggaa gcatgatttt
     4681 ctgtctgtca aagtcactaa acaagtacca ccagttttgc cagctacaat atcggctttc
     4741 caatgaccaa aatcacttct atcatctgcg gattttggac ggccatggat cgaatgagta
     4801 aggcaaatct ttccacgaga ctcgacatga ttttttgtat gtcttgtttt tcctcgatgg
     4861 tgcaatgaac ggatcaggcc tttattgccg tgtgagaggg gttctgtatc gaaaagatca
     4921 ttgcaaattg cacgttagat cgtgataaaa ctaattgaaa agtccatatg ttcatgcttc
     4981 aaacgattcg agatttcctc tggcgaccac tgaaaatcga caaataaatg acgaacaatt
     5041 gaccaaactc acggatcatt tagaatgtga taacgtccgc atttttttct tcgataagca
     5101 taattttttt ccgctttgga aggtgaatag tttttttctt gtttcttctc agttcatgta
     5161 aaatagtaaa cgggctatgc tgaatgtcgt tgccaattat actaatagaa aatccttctt
     5221 cgtgaagcag aaagattctt tctcgttcga gtatagtaag atgacgatac tggctcataa
     5281 ttattttcct tctaggagtg ttctcgacaa acttatacta gttggatatc gattatgact
     5341 catttttatg tgttgcactt aaagtgtaaa ttcaaggtat agaaagaggt tccctatgaa
     5401 aaaaatacct attacaatct taactggcta tcttggttca ggaaaaacga tacttctaaa
     5461 tcatattctg tacggtgatc acggaaagga aattgctgtc attgtaaatg acatagggga
     5521 agtaaatgtt gatggggtat tagtcgacaa gaaaggtttt aaaagaactg gagaaaaact
     5581 tgtttcgatg ccgaatggat gcatttgttg tacattacgc gaagatttga ttgttgaact
     5641 agatcaatta gtgcaatttt ctgacttgaa tcaaattgtt attgaagctt caggaatcag
     5701 caaaccaatt ccaattattc aagcaattat attagctaaa aaccctttag acattgattt
     5761 gacccaacga ttagagattg gtgccattat tatagtagtg gatgtaaaac gtttttggga
     5821 taatttcgaa acgggtgaaa agattcagga aagaaaagct gaaggtaatg tagttgaaga
     5881 agaacaggat attcgtggct tgttaattga tcaattcgag tgttgtaaca tattactttt
     5941 aaataatagt gacttagtgt caaccaagac gatttacaaa attaacggtc taattgctaa
     6001 gttacagtcc aatgccaaaa taattaggac aattaaaggt gaagtagact tatcgaaaat
     6061 tctaaataca aatcttttta atatagatga gacttcctat tcagtgggct ggttcaaaga
     6121 gttagagctt ggctatcaaa atcatattcc tgaaacagaa gaatatggta tctcaagttt
     6181 tatctatcga aatcggttac tccttgacgc acaaaaattt gcccatctta cggataaaaa
     6241 ttttcctggc aatattacaa gagcaaaagg gtttctttgg tttgcccaac atagtgattt
     6301 ttcgacgatc tttgaaacat cgggtagcta taaaagtatt gaatcattaa attattgggt
     6361 agctagttta cctaaaattg agtgtcaaat gattatacaa aaaaatcccg aattgaagga
     6421 agcctggcat ccgttgtttg gtgaacgcat taataaacta gtttttattg gtatgaagat
     6481 ggatcaacaa tgtattaagg aacaacttga gggttgttta actgattctc ttaatgcaga
     6541 taatttccga aaaatttcac catttccatg gaaaaagtct aatatgttta gccagatatc
     6601 gttaatgaaa tttttaagaa aagctggtgt caagataggc gatgtcataa ttattggcat
     6661 tttgattatt acctctttta ttcccttatt tgtattctct atagaaaagg agaatgaaac
     6721 aactgaagat gctatcaaat atgccatcgt tcgaattgat ggcaaggaaa tggatcgctt
     6781 cgatttggat aagatcaatc ataaactcgt gaattatcat cctgcgaagg gacaatataa
     6841 tattgttgag attaaagatg gaagaattcg agttaaagaa gataatagtc ctgatcaaat
     6901 cgcggttaga actggatgga tctcaaaacc cggtcaaacg agtatttgtt taccccataa
     6961 gttagttatt agtattgaga aaaaagaaag cacagattac tacatttact aatctggaaa
     7021 tagatgagga ggtttttaat aagaaaaaga tattattatt aataatagca ggggctttat
     7081 taagtgtgtt aagcgcttgt ggacaagaaa aagaagcggt gacaacaggc acaagtatct
     7141 ttaacgggac ctgcaatggt ttagttaaca gtcggttaga ttacttattt tggctcttcg
     7201 tcaatgagtg ttggtgaaga aattcaatag aataatcgag atactcacac ggctagacag
     7261 ctatcttgtc tagccgtgtt gttgttctgg ctttagtcag ttgggtagta ttcttagttt
     7321 tcttaaaata ttttcctcga tataaaaaga ttcgttctcg gtagttttga aagtttctat
     7381 agccataggc aattcgtttt agtactttga tatggttatt caaacattct atagggccgt
     7441 tggaataggg aacgcgcaac gctcgtttga tttctgtttg gtatttcttg aaggtcgtga
     7501 acactggttg gtagtgctct ggcaactcct tgtagtcttc ttttaggagc gcgacgaatt
     7561 caggaacatc ttgtcttctg ataacggaaa gaaaatcctg gtagacttcg tagcctcgct
     7621 ttaaggaatc atcataagat aatagccgat cgactatctc tgcttctgtc agatgatctc
     7681 gaaatgacgg acgccagtag cgatgctgac ctttcaattc ccacgcattc ttttgaagta
     7741 gtttccagta tttctttaag tgcttcgacc gacgatcccc ttttttaaag gtcttcatta
     7801 cttgaattcg gtggttggta aaagcccgac caatgtgttg gctaatatga aagcgatcgg
     7861 cgacgattcg ggcattcgga aaacactctt tcaccaatgt gcgatagggt gtatagaaat
     7921 ctgaaaccac tagacgcact tgttcacgga cttttatagg gtagcgcaag aaatagtcgc
     7981 gtaaaggatt gagttgccga ttttccacaa tatcaatcag ttgatgggtc ttgccgtcca
     8041 tcatgacaaa gctcatggct cccgagacat ttttcactga tttgaattca tcgaaacaca
     8101 ccacttctgg taacagctgg ctcaaagaaa tggttttagg ccgaaggctg cgaagtattc
     8161 gaatgacagt ggtcggagaa atgtgcttca tacgtgcgat caaagacata gaaagaggtt
     8221 cgcttaatag ctcaagaatg gcttgtttga ctctgcgagc aatggagcag cgacgatcac
     8281 taactgaatc ttctgctaaa aaagtctttc cacagtgttt acatttgaag cgctgtttgt
     8341 taatccgcag aatggtcttg tagttactga catcattcaa taagatggat acttttttcc
     8401 agccctattt aatgatcgtt tgttcatttt gatagtggcg atgaaagcat tcctccggtt
     8461 gataggtcaa tgtgccatca aaaacaaaac atgtttcgtc attgattcgc tctttgcgaa
     8521 aggcatcttc atgaaagata agatttaggt ctagaatatc tagtaactct ttggtaaact
     8581 ctgaatagga catcttgaaa cactcctttg aatggttttc gtcgacttta ttctaaagga
     8641 ttttaaggtg tctgtttgcg tttattagaa aaaagaaaaa gtattggcaa cagaaggcgt
     8701 caccaacact agatattata gatcccttat tttcttcgct tcaaattgtc aaggagtcat
     8761 cataaatttt gtatggattc ttttgtttgg gtagtagtct tctaaataat cttggattaa
     8821 caccttcatc tctattttac ttttatattt gttggagtcg attaattctc atttgattga
     8881 tcgatgaaac gcttcaatta ctgcattgtc aacaggagtc ccagtttggc tcactgaatg
     8941 tgtgatgtgg tggtcgttca acagctcttg ataaataaaa gaccgatgct ggctacctct
     9001 atcggaatga attaccgtag gcaattctcc ttaataaatt tgtaatggac ggacgacaat
     9061 ctcttttttc atgtgtgaat ctaccatata gtaacgtaga atacgagttg ctaagtcaat
     9121 ataggtggac aaatagaggc gaccgtcatt acatggaatg taagtaatat ccatcaccca
     9181 aacagcatca atttggttct gttcaaatta tcgattcaac atttttttac catttctttg
     9241 attgtttttt ctctaccatg agaagatttg cgtcgatgaa atcctcttgt gcataaattc
     9301 atttcacgca taagaacagc tacaacacgt ttattgattt cgattccttc atcgtaaagt
     9361 ttttgagtga tccctggaga tccgtatcga tctttgtgtt cgtagaacac tcgtcttcct
     9421 cttttagtcg tttattttcc tgttcaagaa tgtagaaatc tgctttatca ggtaatcttt
     9481 ttccttgtcc agcaaaagca tttttcccgt atcgacgata ttctgaaacc catcgggaaa
     9541 ttgttggacg agagatattg tattcttcag caattttctt ggtagttact tttttctcaa
     9601 ggatatcctg tgaaatcttt agtttaaatt tacgatcata tgtttttctc aatagagttc
     9661 attccttccg gtaacgtaac tgatagtcaa gaaaagtgta tcatagccca acttagtatc
     9721 agggtttgtc aactaaactg tggaagttga tagataagag tttttaacaa ccaaaattct
     9781 cttgactatt tttgaaatca gacaaatttt gattggacac atagttgaat aaatctaccg
     9841 tcgttacgga aggtaaatcg catgcttttc aattctagag gagaagtcct cgttgaagta
     9901 ccatagccca gttctggatt cgacctcctg cccacttcat acgtaaataa agtaatttta
     9961 ggagtgagac tgtgacaaaa gtt
//
LOCUS       DACDMM010000098         9930 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDMM010000098 DACDMM010000000
VERSION     DACDMM010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.099"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(24..1535)
                     /locus_tag="IWD16_001920"
     CDS             complement(24..1535)
                     /locus_tag="IWD16_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9088931.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(1857..3209)
                     /locus_tag="IWD16_001921"
     CDS             complement(1857..3209)
                     /locus_tag="IWD16_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP9088932.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(3362..4438)
                     /locus_tag="IWD16_001922"
     CDS             complement(3362..4438)
                     /locus_tag="IWD16_001922"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP9088933.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            4634..6379
                     /locus_tag="IWD16_001923"
     CDS             4634..6379
                     /locus_tag="IWD16_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP9088934.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(6659..7096)
                     /locus_tag="IWD16_001924"
     CDS             complement(6659..7096)
                     /locus_tag="IWD16_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP9088935.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(7080..8453)
                     /locus_tag="IWD16_001925"
     CDS             complement(7080..8453)
                     /locus_tag="IWD16_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088936.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(8775..9263)
                     /locus_tag="IWD16_001926"
     CDS             complement(8775..9263)
                     /locus_tag="IWD16_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9088937.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(9285..9887)
                     /locus_tag="IWD16_001927"
     CDS             complement(9285..9887)
                     /locus_tag="IWD16_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9088938.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         2902 a         2118 c         1796 g         3114 t
ORIGIN      
        1 aaagagaaac tttcatttta tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt
       61 ttatcattgt gacgtttacc gccatctttt tggctgttat tatttttgct ataaccgcct
      121 tttgatttgt ttccacgata attaccgccg tcacgacgat tacggttgtt tccgccacca
      181 cgtttgccat ttttattgta tccttttttc tgcattggca atgggcgttc tggtgtgatc
      241 ttcactggca ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa
      301 tcttgtgcag aatattcttc taataattta tctgctgttt gcaggtattt atctagacca
      361 ttttctgcaa gttttgtttc gatttgttca actgcagcac ctaattgacc tttgaatgct
      421 tctttttcgg ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca
      481 tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag acataccgcc ttttccggca
      541 cggcctgtac gtccgatacg gtgaacgtag ctttctggat cttgagggat atcatagttg
      601 tacacatgag tgacacctga aatatctaat ccacgtgcag caacgtctgt cgctacaagg
      661 atatctaaat gaccgctctt aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa
      721 tcgccatgga ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc caattcgtct
      781 acacggcgtt ttgtacgtcc gaagacgatt gttaattcag gtgtttgaac atcaaacaag
      841 cgagtcatga tatcgaattt ttcgtattct tttgcgcgga catagtattg atcgatcaag
      901 tcagctgtca tttctttcgc tttgatcttc acatgatgtg gatttttcat gaatttaacg
      961 ccaatattct tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca
     1021 ggaacttgtg aaatgatttt ttcgatatct tccaagaagc ccatattcaa catttcatct
     1081 gcttcatcca aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg
     1141 tcaagcatac gtcctggtgt acccacaacg atatgtggac gatctttcaa gccacggatc
     1201 tgacggccaa tatctgctcc gccgtaaacg gcttgtacac gtactttttt gtcgcgccct
     1261 aaacgatata attcttcttg tgtttggata gctaattcac gtgttggtgc gataactaaa
     1321 ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc aaatgcagct
     1381 gttttccctg tacctgtctg tgcttgtcca ataacgtctt ttccagctaa tgctagtgga
     1441 atcgtttctg cttggatagg cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac
     1501 aattctggtg ataattctag ttctttaaat ttcaaatgaa tcctcctata tggttgttaa
     1561 ctgaggatgt agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt
     1621 tttgacagtg gccacgcacg tttttatcca ttcttagccc ctgtggcacc accgaaaaat
     1681 cactttgcga agtttaggca ttagaaaagc caaacgggtc gcgttcagct ttatcattat
     1741 tatgacatct ggcttcaaca agttgtttct atggcaaaac cagtccaatt tctcttcatc
     1801 tttcggcaat aggttattct aacatagttt ttcgttttct gcaagtttac agatttttat
     1861 ttcatttgct gaagttgttc taccacttca aataagccca tgctgttgct gccttttagc
     1921 ataatactat ctgttgcttc aagattttgt ttgatagcat caatcaattt attcttttct
     1981 ttttcaaaat agaacacagg tagttggggg tatttttggt tcaattcttc ttttagtgca
     2041 tgcatttgtt ccccataaag gaaaatcatg gcgaatttct caggatcgat atgttcactc
     2101 atttgcgcat gcatatttaa agactctttc cctaattcca gcatatctgc taacacagcg
     2161 attctgcgtc cttttgttgg cagattttta aaagtatcta ataccagtcc catggctgtt
     2221 ggatttgcat tgtagacatc gctcaaaata gctgcaccat ttcctgccgt tagccattcg
     2281 gttctgtttt gagtgataga aacatgggcg agcccttggc gtatctcagg tatggacagt
     2341 ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg ttacattata acttccaggc
     2401 agcgggatcg taaatgtttc tccttctact acaaaatcgg tttgtgcttt tccttttgct
     2461 gtcacttccg catgaacatc gccatttcca ataccgaatg ttgtgactgt ttgtactaat
     2521 gttgccgtta aaggtgtcag caacggttca tctgcgggca caacgagtaa tccgtctgat
     2581 acaagtccgt cagtgatttc cattttggct ttagcgattc cttctcgcga cccaagattt
     2641 tctatgtgtg cttcaccgat cattgtgatt gctgcagctt ttggttgagc cataagtgaa
     2701 agttccgtga tttcattggc gtgatccatg cccatttcca aaacgatctt ttctgtatct
     2761 tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc
     2821 ttatatgttt tgtatttttc agacaagaca gacgcggtta gatctttcgt ggtggtttta
     2881 ccattgcttc ctgtaattgc aacgacttct gcgcctactt tttccaagta ataggttgcc
     2941 aattgctgca tggctttcaa aggatctgct acaggaataa ctggaaaatt ttttggagct
     3001 tgttcaacag gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc ttgctggata
     3061 aattcatgtc catctcgcgt accagcaaga ggaacgaata aacttccttc ggttatcttc
     3121 cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag agcattcgct gcccacggct
     3181 tgtgcaattt cttgtattgt tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa
     3241 agcgtttggc ctaaacggcg ggttcgcatg actctcactt ccaagtaaat ttctctattc
     3301 gtatttagaa gctagaatga acgcggttac gcaatcactc cagcctcttc tttatttctc
     3361 tttattcatt tttttcaaaa aaagattgac gctgatggta tcgattcatt cctaactgga
     3421 tcagttcttc aatcaaatca ccgtatttca agcccatatt ttcccataag agtgggtaca
     3481 tactgaactc cgtaaatcct ggcatagagt ttaattcatt caggaataat tcatttttat
     3541 ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc taacatcgtg taagctaact
     3601 tcgcgtactc ttgcgctttt tgataaactt cttctggcac ttcggctgga atctgcattt
     3661 cgattttatt attgatatat tttgcttcat aatcatagaa tgctacgtct tttacgactt
     3721 caccaggcaa agtcgtccga acgtcttcat ttcctaatac agcaacttcg atttcgcgcg
     3781 cttcaattcc ttgttcaacg attgctcgag aatcatactg ataggctgtt gctaaagcat
     3841 tttgcagctc ttctcggttt tctgcttttg taatgccgac actagaaccc atattcgccg
     3901 gtttgacaaa catcggataa agcaaagaac cttcacattg atcaaatact tttttaggat
     3961 tttctttcca ttgattctta agtactggta cataaggaac ttgcggcaca ccagcagctt
     4021 gtaaaatata cttggtcatg attttatcca tggcacatgc actggtcaat acgcctgcgc
     4081 cgacataagg catattcaat gtctctaaga agccttggat cgttccatct tccccgtttg
     4141 gcccatgtaa aactggaaaa acgatggctc cttcttcttt gatttcgccc ggattgatca
     4201 cttttcctgt aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat
     4261 ctttgctggc aggtttttct gttagtaatg gacctttgac ccattgtcct tcttttgtaa
     4321 taaatacgag ttgaacttgg taataattat aataaatggc atttaaaact gaaaatgcgg
     4381 aaagaatgga cacttcatgc tctgcgctgc gtccgccata tagtaaagta atcttcaaaa
     4441 gattgtcctc ctgtattcta aatcgttcag tgatttactc tcatatttta gcacaaaaca
     4501 aaaataaacg aataacttat ttataaaaaa acgagccagc ttctaaaaga acgagcgtca
     4561 tttatttttt gtcagctaga ttatttgtcc cgttttttat aaaaagttat gataaagaaa
     4621 aggagggatg atgatggcgc aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt
     4681 gttcatcaat tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa
     4741 aaaagccttt gaagaactag gccccacctt catcaagata ggacagatgc tttctgttcg
     4801 agaagatttg ctttcttctg ccttcactca aacattcaaa acattacaag atagtgtacc
     4861 tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc tttcattatc
     4921 agatatattc gatgattttt caaaatcgcc ttttgcctct gcttctatgg ggcaggctca
     4981 tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag atccaacacc ctaacatcgc
     5041 cgaagagatc cgacttgatc ttcaactatt tgaacgagcg atccctttga tcaaatacat
     5101 tcctgaaact agcgtagttg atctaaaagg ggtactccaa gaagtaaaac gttccttgat
     5161 caatgaaatg gacttcttaa aagaaagtca aaatggcgaa caattctatc aaaagaacaa
     5221 tggctggaaa gagatccgca gccccaagat ttatgacgct ttttgttcga aaaaagtgat
     5281 cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta atgaacgccg aaaacaaaac
     5341 tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa caagaagttg caaagctttt
     5401 agttgagaac tttatgaaac aggtgtttga tgatggtttt ttccatgctg atcctcatcc
     5461 agggaatctg ctattccatg tactaacaaa agaagaacag acacaagcta gccgaaaaac
     5521 tgaaacagtc catgaaaaag agttcggttc ctttgctttt cgagcctcca cttctgcaga
     5581 agaccctgtt gcgccatata ccatcaacta catcgatttt ggtatgatgg ggcatctttc
     5641 tgcaggactt aggcaaaaac tcacacaagc cgttttagcg ctgtatacga aagatgctta
     5701 tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg
     5761 gttccaccaa gaactgacaa gttttcttga acagtattat gattctccta ttgatgaaat
     5821 caacttgcaa gaagtattca cacatgtcgt gacgatttgt catcaaaata atctgcagtt
     5881 cgatcgtgat atcacgcttt tgctgaaagc atttgggaca ttagaagggg tgatccgcgt
     5941 cctcgatcct gaagtttccc tcatggaagt cgcttcgcct tttgctcaac attattttct
     6001 cactcatctg gatgtagaag atacattgaa acaaagcgga ttagacttat tggaagggat
     6061 gaaggctgcg cctaagatcc ctcaacagct ccaccatcta ttagaaatgt ggacttcggg
     6121 acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag
     6181 tatgatcaat cggcttgttt tcggtatgat tttagctgca ttgatcgtag ggtcctccct
     6241 gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt tccttattag ggatatttac
     6301 gtatgctatt gccgcattcg tcattatttt tttggcaatc gatgcattga tccaaatgta
     6361 taagaagagg aaaaagtaga aaaaggttgt gacagaaaga atcagcttca agaaataaga
     6421 aggggtttta cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga
     6481 ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata
     6541 agaacaatca aaaagggaac atggaacaca tttatcctgt ttccctgttc cctttttgat
     6601 gtattcgagc ataaataaaa taccttcgtt acctactatc ttctgtactt ggtaaacctt
     6661 agtcaattgc cttttttaac gcttgacgaa aagacatatg atctttctcc gtaaagggtt
     6721 ttggaccctt tgtccttatg ccagcttttc ttaactttcc actatgataa cgttgttcta
     6781 gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga gtgatggaac actcgcacac
     6841 ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag tcattcttct
     6901 taaccatacc gacgatttta taatccgcac tatcagctcc gcggtctacg tagacgattt
     6961 ggacatgatt agcgtattct ttggtcgtat aatggtccac actggtcacg ataaccacgt
     7021 caagaccata ggattctcct aattttatga catcttcttt tactggagaa ccatctccat
     7081 caatgaatag cctcattctt tttcgccttc ttccataaat gataaacagt tactaatccg
     7141 ctcagtaaaa gaccaacatt gatccatttt gccaagataa gcagttgtac actttctatc
     7201 ggcactgatt catggttcag cgtgatcaat aatagatgga ttggctctcc catttctttt
     7261 gctaaaacaa acaaaagcgt catcaataaa aactggtgga tcacactccc tttattttga
     7321 gtagtatact tgacaacgat ccggcgatcc attggaatag ctgtatcagt ttgataatat
     7381 tccttattta gccagctgcc tgttactacg tgccagaaac taagcagaaa gacacctatc
     7441 gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac tcagtccaag caattgcata
     7501 gccaatggag accatgttaa acgtttcaaa atccatggtc cgactatttt tgctgcaatc
     7561 aagcctgctg cataaggcaa atacataaaa atcgaaagtt tttctaatcc gtacagtatg
     7621 ccaacataaa aactgctaaa taaaaaaagg aaattcccta gcattccatt tagaaaagta
     7681 aacagattta gccagcttgg caacttccat tccttcttct ggtgtcctaa aacaaacacg
     7741 aacaagatga acagcagcaa gaaaccataa atagcaagat caaaaagcct ttcatcgaat
     7801 aacaatcggg catttctcaa aagaaacaat aagccaaaga atatcaaaaa taaaacaagc
     7861 tctcgcgtag ctaccagatt tcttttgatg tctttgaaat ccagttcgta acgcggataa
     7921 tgactaaccg tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca
     7981 atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca gatagctgca
     8041 aataagtact ttttcccatc catattgata aaaccttgcg tcttttcgtg aaaattgacc
     8101 gtggtattag caggtacaag ccatgctgca ctcagcccta gcaaaacact tgaaaaataa
     8161 aataaaggaa aataaaatac gcctaatatt ccaagcaatg aaccagcacc accaatcagc
     8221 agagcgatca tcagcaacgt gtaactatct agaccaaatc gtagactgtt gactaaaaat
     8281 agtccagtca tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag ccaattatgc
     8341 gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt acttaagata
     8401 aagggcgttg tttgtgtaaa attacgccca aatcgtttga gtttttcgaa catgacaatg
     8461 gctccttaaa cagtcttata actaatctgc tcttcgattt tagcattaaa ggggaaataa
     8521 accaatttta cagaatatat tcttgtaaaa aaaaatacag aaagaagcat aagcttcttt
     8581 ctgcattttt acggtacttg attactcaag cagacaagcc cttctccaca aaagtcttgc
     8641 ccgacacccc tgcatccggc aggtgataaa gatagaaatc aattatcctg tgcctccaaa
     8701 atcaattagg agctctgtaa accagagtcc ttctcgaccc gtcgcataaa tttactttaa
     8761 cacagttcct atgtttatgc gagagctttt ttcattttat aatcgaccat ttgaaatcct
     8821 tgaccgatat ctgaaataag ttcttctgta atctcaaatc cttcatgctt ataaacctcg
     8881 attgcttgat gattcccctg attcactcgc aaaaagatct ctctttcctt tgtttgcgca
     8941 gcaagttctt cataccagcg aaaaactgaa gaagtcaatc cttgaccacg tactttattt
     9001 agcaaatata atttacttaa ataaattttt ccatttatct cttcataagc agtgtaaccg
     9061 acagtctttt cttcgctttt taataaaaag taggacacac cttctgtcag ttctttttga
     9121 atctgttttt ttgactgata agttttcagc atgtaatcca cttgtttttg accaatgatc
     9181 ggcacaaaaa cttccggcca tacttcttgt atgactgcca tcagctcatc cagttcattt
     9241 tctgttactt ttattttttc catacaccca cgtccttttt tacattataa atgaccttgc
     9301 gcttgatgga ataaaaagag catgatcatg ctatacacac ccaccgacca cggtttcaag
     9361 aaaatcacaa tctgcataaa ttccctaaaa gataatttcg tcaaaccagc tactagacag
     9421 atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa ctagtacttt tttaatatct
     9481 ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact tttttggcga taaaataagc
     9541 tggacaaagg ctacgccata gtaacggaca agcaaaaatc cgattgcttc cccaatcact
     9601 aggccaatac aactatataa aagcccaaat ccattgccaa acatcagcat gcccgctact
     9661 gaggagatcc ctcccggcaa aataggaatg atcacttgga tcacttgcaa cagaacaaat
     9721 atcatcactg catacttgcc aaattgctgg ataaacgctt gcaaagacgt gctcgatcga
     9781 aaaatcccat gatgataagc ataaagaatc aacacaaaga aaaaaaacag accaataatg
     9841 ggcatgatct gaagacattt ttgtaaaact gatccttttt tagacatcta tattccccac
     9901 atcttttttc atttattaaa actgcctcct
//
LOCUS       DACDMM010000099         9649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDMM010000099 DACDMM010000000
VERSION     DACDMM010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.100"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            34..657
                     /locus_tag="IWD16_001928"
     CDS             34..657
                     /locus_tag="IWD16_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088939.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /locus_tag="IWD16_001929"
     CDS             1107..2156
                     /locus_tag="IWD16_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9088940.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="IWD16_001930"
     CDS             2153..3034
                     /locus_tag="IWD16_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9088941.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="IWD16_001931"
     CDS             3037..3807
                     /locus_tag="IWD16_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9088942.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="IWD16_001932"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="IWD16_001932"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP9088943.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="IWD16_001933"
     CDS             5118..6281
                     /locus_tag="IWD16_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9088944.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="IWD16_001934"
     CDS             6398..6808
                     /locus_tag="IWD16_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9088945.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="IWD16_001935"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="IWD16_001935"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9088946.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9348..>9649
                     /locus_tag="IWD16_001936"
     CDS             9348..>9649
                     /locus_tag="IWD16_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088947.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVF"
BASE COUNT         3351 a         1638 c         2076 g         2584 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttca
//
LOCUS       DACDMM010000100         9586 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDMM010000100 DACDMM010000000
VERSION     DACDMM010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.101"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            26..211
                     /locus_tag="IWD16_001937"
     CDS             26..211
                     /locus_tag="IWD16_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9088948.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            223..471
                     /locus_tag="IWD16_001938"
     CDS             223..471
                     /locus_tag="IWD16_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088949.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            502..681
                     /locus_tag="IWD16_001939"
     CDS             502..681
                     /locus_tag="IWD16_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088950.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(724..1194)
                     /locus_tag="IWD16_001940"
     CDS             complement(724..1194)
                     /locus_tag="IWD16_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088951.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1281..1979
                     /locus_tag="IWD16_001941"
     CDS             1281..1979
                     /locus_tag="IWD16_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9088952.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            2157..2498
                     /locus_tag="IWD16_001942"
     CDS             2157..2498
                     /locus_tag="IWD16_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088953.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            2491..3162
                     /locus_tag="IWD16_001943"
     CDS             2491..3162
                     /locus_tag="IWD16_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9088954.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            3168..3854
                     /locus_tag="IWD16_001944"
     CDS             3168..3854
                     /locus_tag="IWD16_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088955.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLSH
                     ETIKKLRIGG"
     gene            3857..4687
                     /locus_tag="IWD16_001945"
     CDS             3857..4687
                     /locus_tag="IWD16_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9088956.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            4703..5554
                     /locus_tag="IWD16_001946"
     CDS             4703..5554
                     /locus_tag="IWD16_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9088957.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            5551..5910
                     /locus_tag="IWD16_001947"
     CDS             5551..5910
                     /locus_tag="IWD16_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP9088958.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            5925..6086
                     /locus_tag="IWD16_001948"
                     /pseudo
     CDS             5925..6086
                     /locus_tag="IWD16_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            6083..6388
                     /locus_tag="IWD16_001949"
     CDS             6083..6388
                     /locus_tag="IWD16_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088959.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            6388..6699
                     /locus_tag="IWD16_001950"
     CDS             6388..6699
                     /locus_tag="IWD16_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088960.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            6866..7282
                     /locus_tag="IWD16_001951"
     CDS             6866..7282
                     /locus_tag="IWD16_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP9088961.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            7282..7950
                     /locus_tag="IWD16_001952"
     CDS             7282..7950
                     /locus_tag="IWD16_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9088962.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            7947..8324
                     /locus_tag="IWD16_001953"
     CDS             7947..8324
                     /locus_tag="IWD16_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088963.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            8321..8620
                     /locus_tag="IWD16_001954"
     CDS             8321..8620
                     /locus_tag="IWD16_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088964.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            8862..9278
                     /locus_tag="IWD16_001955"
     CDS             8862..9278
                     /locus_tag="IWD16_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9088965.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         3521 a         1439 c         2130 g         2496 t
ORIGIN      
        1 atttttttag aaaggagcta tttttatgtc tgagaattta gacttaaaaa ttcgagcgga
       61 gatgagaaaa agaagaatga ctttcaaaga actagctgcg cttatcggta tttcaggagc
      121 ttatttatca gatattctaa acggcaatcg tgatggaaag aaagcacaac agcatatcga
      181 aacagtgaaa aaaatattgg acatccgata ggggggtagg cgatgacaaa actaaaaaaa
      241 caagattttg taaaaaaata caattattct ccatctactt atcaacgtcg aatgtcggaa
      301 ctaaaaaata cagcaatttt ctcagcggcg tatgaacggg tcacaggaca agaagtttgg
      361 atcaatacag aattatacga taaatttttg tctttcaaat cctataacag gttacgcaca
      421 agaaaggtaa cacctaaaga atttatcgag aagcatttag ttgatttata aaaaacagaa
      481 cattttgaga ggtgaaggtt aatgggtaaa ttcaacagag cattggtatt cagcgcaccg
      541 ctaatcatct acgctttagg actttgggga agcaggcaag cgttgatagg aacgattgtt
      601 tacatggttt ggatttttat ggggcttgat gaagctgaag ctgagtacag agcgaaaaag
      661 ccaaccgagg gggctgacta aaaatgaata gaaaagagaa actagaatgt atattactat
      721 tactcagttt aattctttca ctaatttctc tattgggaag tttgtatttt tgatatcaac
      781 aattttgtgg atttgggttc tatttagagt aaatatcatt tttgtttggt gattaaggag
      841 tttattaaaa ttaattttcc ctacttcaaa tgcaaggtaa caagatatag cacttttagg
      901 aggaatattt agcggtacag cttcagagta tattctatta tcatcgactg atcctccttt
      961 agcaattaat ttcttaaatg gagtagctga ccatctgcgg tttaagttag cggatgaaag
     1021 ttctaaatta actaacatag ctggttctgt tgaatagtta gaaatgatta cttttgtata
     1081 aatcatatct gcagcgaagt aagaagcatt aagttcaact tctagttgag ggcgcattcc
     1141 ttttattttt atccctgtta aaaacgtact caatataaaa cttattattg acatccattg
     1201 aaaaattgtt aagtgaaaaa aattcaaaat aaacaccacc agtttttaac taaattatac
     1261 caaaaaggag agaagaaata atgcaagaat tagtaatttt gaaaaataaa gaagctgtga
     1321 ctacgagctt acaagtggca gaaagcttcg agaaaaaaca tcaacatgtt ttaagagata
     1381 ttgatgcact aaaaaaagat gtgtccaatt ttggacagat gtttgtagaa ggtaatgaac
     1441 cagattcata tggcagaaat cgacgagttt tcttcattag tagagatgga tttttcttgc
     1501 tggctatggg gtttacagga aagaaagcta tctacttcaa acaaaaatac attgaagcat
     1561 tcaacgaaat ggaagatgtt attcgcaaga atactgttcc tcaaacaatt gaagatatga
     1621 tgatctatca attagaagaa atgaaagatg ttaaaaaaga tgtttccatg cttaaagata
     1681 ctatgcgaat tagcggacaa caagagtttg aaattaagca aaaaggaaat atgaaagtta
     1741 tgggagttct agggggaaaa gaaagccgag cttatgaaga aatcagcaaa aaagtattct
     1801 caaaattttg gtctgaattt aaacgtacct tttcaatccc aagatatggc gagttacctc
     1861 gtaagagatt cgatgatgct gtttcattta ttgaaatgtg gttgccagaa actgcgatcc
     1921 gtatggaaat tgatcaactg aacagacaac agagactttt tggtgatgac aatgaataga
     1981 gctgaagcgc taagaatagg gacggcaatt gctaattgct ggtggaaata ctataaacca
     2041 atcatcctaa gccaacaaca tattgacaag caaaaagcat ggcaacaaat aaaaaagtga
     2101 ctccgccggc aagcaaagag tcacaaagaa aacacatcat aaggagattt tagcatatgg
     2161 aaaaagaact ttccactcta gatcaatatt tgattgatcc tgattggggc aagccgaaaa
     2221 ttgaggaaac aagtggtcga aaaatcagac gaaatctttt gacgaatgaa gaactagctt
     2281 gggatcaaga tgatttaggc aaccatgtaa ctatttggga tcatgtttat cttatccatc
     2341 tatcgaagca ttcgaataaa cctgaatata tttacgtcat cgaagatggc ttgattgatg
     2401 cgctagaaga gtacgacaga gataacttga ttgatatctc ttattacgga ccaggtaaga
     2461 aatacattgc tgaaatggag gcagaatttg atgagtgaaa tcaaagggac aacgaacttt
     2521 gaaaaacttt ttagtcgtaa gttaaataaa attctcaaga aaaaaggaaa ttttgattat
     2581 ttatcttggg ctcacgcgtg ggagattatg aaaaagaatg atccacaggc aacggtaact
     2641 attaatgagt acaaacacta cagggttgtt tctggaactc atcaagactt tcttgttgag
     2701 gaatataaac cttttcttat ggatgaaact gggacttatg tatctgtctc agtaacggtt
     2761 aaaggacaca cggaaaccga attatttcct gttttagatt atcgaaacca accagttgtt
     2821 aaaccaaatg caatgcaaat caataactca ttgaagcgat gctttgtgaa agcattggct
     2881 ctacacggac tgggattata tgtatttcaa ggggaagata ttccaacacc acctagaatc
     2941 gatacaaaga aattaaacat gctagagacg attctagaag ctttcaatga gcagatgggt
     3001 aaagatatga ccaaaacctt aatcgaatat gttaatgagc agacagataa attagggctc
     3061 ttagctgata acgttgaaac tattgaacag ttaagctatg agcaatgtgc cttgatggag
     3121 cgagcaatag cagctaagag aaaagaatta gataagaagt gatatgagtg tttaaaccat
     3181 taatcgattc atattcagcg gttctgaaaa agttcaaagg aaaagacata agcgcaacca
     3241 tcaatgagga agtgaacatt gatcgactaa agacgatgta tgacggctac gatggtgatc
     3301 gagtcattga aattcgtttt attgatccta gacgtttcac cgtacagcaa cgaaacttca
     3361 tctatgcgct gataggcgat atttttatcg atacaggcat gccaacggac ttctggaagg
     3421 aattcttcta cttccgtttt gaaggtgtca cagggcgcaa aataagcctc aaagatgaat
     3481 cgaatacgac tgtgagtgat gctaatgtct tagcaaatat catcttagat ttcatctttg
     3541 aacatcatat tcctttcaaa gaaggctatg agattttacc agcgaatcaa gaatattact
     3601 tctacaaatg cattacaaaa agagtttgtt gcatctgtgg caaaacagga gctgacatcg
     3661 atcactttga caaagcgcta ggaagacgaa agcgaaaaga agttgatcat tcagagtaca
     3721 catttgcagc actctgcaga atccatcaca cggagaagca caaaataggt gtgatcaatt
     3781 ttaaaaataa atatcaaatc aaaggaatca agttaagtca tgaaacgatt aaaaagttaa
     3841 ggataggagg ataaatttgg ctgagataag ttggatcaaa cttaaaacta ctatgtttga
     3901 cgatgaaaaa atacggttaa tccaagctgt tcctgagtcg gatgccatca tcgttatatg
     3961 gattcgatta ctagttttag caggaaagac taacgacgat ggtctgatat atatccagag
     4021 gaacatgcct tataccgaag aaatgcttgc tacattgttt ggcaaaaacg taaatacggt
     4081 tcgcttagcg ctaactacat tggcaaattt caacatgatt gatctaagca gtgatggact
     4141 aattgccatc agtaattggg aaaaacatca aaatatcgag ggtatggata aagtaaggct
     4201 aaaaaatgct gaaagaaacc gtaaatacag ggaaagaaag agacaggaac gtctcaaatt
     4261 ggaaaatgac gttagcgtga cgtcacgtga cggtacagat aaagatatag aagaagataa
     4321 agatatagat aaagaagaaa agaaaggtaa gtattctgac gaacacttac gccttgctaa
     4381 aaaaatgcaa aggaatttaa ctgaagattt tccaaaagaa atgaacaaag tagatatcga
     4441 aaaatgggca gacacaatca ggttgatgga agaaagagat aaagcatcta tagaagcgat
     4501 tgagtatgtg atcaattggc tacctacaaa tgaattttgg tttggaaata ttagaagtgc
     4561 taagaaattg agagaaaaat ttgagaagct caaattcgaa atcaaagcag acaagaagaa
     4621 tcataaaaag caaagtcaaa aactacagta cagcaatcct agtgaatatg acgacttgcc
     4681 aatttaaaag gagatgcatc acatggaaag cctagcaaat gctatggaga agctaataag
     4741 aagagtatta gtgcaaagcg gaaaatgtcc agaatgtagc gaacctttgt atagttggcg
     4801 agctaaaaat aaggatggtt cagaacgttg taaaccaaca tgtatgagtt gtggttataa
     4861 agcgttacgt gtgaaagagg atatacagac cgaacggata tataacgaca gcttaaaagc
     4921 acgagcattg agtttttttc aaaatggttc ggtattaaca gataaaactt tgtttaaatg
     4981 caaaatggag aattatcacg tagtggacca agaaacgaaa attgctttag aaagagctaa
     5041 aagctatgta aatgatgtcc tactgaacca tcctgcacat ttcattctat cagggaaatc
     5101 aggaagcgga aaaagccact tatcaatggc gacagcttgg gaaatacttg agcgctcaaa
     5161 ttatgacaag aaaatacttt ttataagcta tcaagagtta ttagagcaaa taaagttttc
     5221 ttataacaat gctgaactga gaaaagaaat tgaaggatcg cttatagccg atatcaaaac
     5281 aactgatttg gtggtttttg acgatattgg agctgaatta ggtagcgggg tatcaaatag
     5341 taggcaattt acaaacaaca cgttaaacac gctcttagaa gccagacaga acaaggcaac
     5401 gatcatcaca acaaacttat ctggtcctga actaagagaa gcctacggtg aaagaattgt
     5461 ttctaggata tttaagaatt cagaaggtta tgcgctgaaa ttccaacaaa cagcagacaa
     5521 gcgcataaaa ccagtgaaag gtagtatcgc atgaataaat accgtaataa aaaaactgtt
     5581 catcgaggta tcaagtttga ttctatcgca gaagcagagt attacgatct agccttgtgg
     5641 caagctgaag cgaacggctg gaaagtaaaa cttcaggaaa gatttgagct gatgccgaaa
     5701 tttgaactag acggaaagaa gtatcgcaag atcgagtata ttcccgactt cacattttat
     5761 aaaaacggca aactagtcaa agtcgtagat gttaaaggga tgcagacaaa agatttcaaa
     5821 atcaaagcga aactcttctg cagtcaatat cgagtaccac tgatcttagc gaagaaatat
     5881 cggaatacgt tcaaggaaga acggttttaa cgaggtgatc catcatgaca acagaagaag
     5941 tgattcaaat gcgtattcga agccttcagc gtgagattga cgatctgtaa cggacaaagg
     6001 cagtgatggt caatgaaacg gcgaggaagg caatcgattt gcacatagaa aatttaagaa
     6061 ggaaaatcca tcgattggag gaatgagcgt ggataagaaa gcagcaatga aacgaatcat
     6121 cgaactgaca cattctgaga attggcaaga agacaaagaa atagttgcag aagtccaaaa
     6181 gctcggtaaa tcaatgtggg ctgaaaagcc taaacggaaa acgccgagaa aaattgcaat
     6241 ctggcatggt gatcgaattc tagtaacagg tactgctgaa cagttatctg aaattactgg
     6301 attaagcaaa aacattatct gggatagagc taggagctta tggattgatt caaaaggacg
     6361 acagtttagg tatgtggagg agaaataatg aacgaactaa tcacaaaagt agagcagtgg
     6421 gctaaagaca agggactgga tcaagcggat ccaaaagcac aatttttgaa agtagctgag
     6481 gaattcggag aaattgcatc ggcaatggca agaagtaatg atgagctatt taaagatagc
     6541 gtaggagacg ttatcgtcac gctgattatt ctttccatgc aaaaaggggc aaacatacaa
     6601 gagtgtttag aaatggcgta taacgaaatc aaaggacgca caggaaaaat ggtagatggt
     6661 gtattcgtga agtcgagtga tttggaggac agcgaatgat gatgccaaaa gaaatctatt
     6721 cagatgcatt aagaggtgct agaaatcaat tgaaaatggc aaaaagagca tacgaaattc
     6781 ggcccacaat cgaaaatgaa cgcagagtaa aagcaattcg ccgtagatgt tcaatttacg
     6841 gcgagttaca gaaggaggac agcaaatgat accgagattt cgagcttggg ataaacgaaa
     6901 gaacgtaatg agagatgtag ccgtcttgca ttttactaaa aacggcaaaa caaactttat
     6961 tgaatattgg ataaatccta ccgaattgaa atcatatcac gtgcgaaaca tcgagctcat
     7021 gcaatcaaca gggctgaaag ataagaatgg cattgagatt ttcgagtggg atattgtatc
     7081 agtcagcgtg cgaaatggat tcgattattt agataataaa gtttgtgttg tcaaaaattc
     7141 aataggacat tcgggattag tgtgtgccac tgttgatgaa gatttagagt atcaaatttt
     7201 taacacagag ctgtttgaag aatacacgta tgaagtcatc ggaaatattt gggagaatag
     7261 cgagttattg gaggaacagt gatgaataaa caggaattga ttgatgaatt agctaaatat
     7321 gtaaagcgtt atgagaacgt tatggatgag catggccaag gaaggtacgg cgcttatgaa
     7381 gtatctttaa agttggtgaa aagactaaat gaatcaaaaa ttacggacga acaagcttgg
     7441 aataaggtag ctgaggctta tcctgaatcg gcacaaagct tgagaaacac tttagataat
     7501 gctgtatttg gtaagactgg tgaaccgcag aagcacgtta tgccgaagtt tgtggcggag
     7561 tggatagaat acgctaaaaa gaaaggtgat agtctagcta tttcattcaa gccgtggaac
     7621 ctctatggtg ttgagtatag caaggctgat agatggattg gagataatca agaaacgttt
     7681 gctcgtgctt ggctagacgg ctacgaggtc gagaaagagc cgttgtatta tgtcccgcta
     7741 ccatatgaag tttgggatga agaggcagcg gagcttaaaa cagagtattt gtatctacat
     7801 tatgagatca caagtgatga aactagaata tttcctacaa aagaacctag aaaaggtttt
     7861 gtcgcaaaat tagatgaact tacgattaaa tcagcagatg aaaactactg gccgtttgct
     7921 gtaccagtgg aagaggtggt agaaggatga aaaaattgca ccaactcata tctgagaaag
     7981 aaagcgaact ccaaaattta gaagatagtt tgggattggg gtttccaatt gttgagcaag
     8041 ttaaaatggt tcaaatcagt catctacaac ttgaattgga agatttaaga caaatagaag
     8101 atccgtatca actcaacgat aaccagcaaa tcgtgttgga gtggttgaaa ctaactgcgt
     8161 caacaggaaa gccaatgcaa gttgtatttt ggatgatgaa taatgcagca tgggggcatt
     8221 tagacgaatt gagggatcct ttgatggaat taactgacaa acagcaattc gaagtcctaa
     8281 cagcattcgc tcaatgggga ttagaacagg aggaaaaaga atgaaactaa aagacggatt
     8341 ttacgctagt agccacggta tcggcggttt aatgctagac atgccgacaa agaaccctaa
     8401 aacacgtaag aagccaaaag tcaaagtcgg tgacatggtt cgctgtgaag cagaaggatt
     8461 catctatcca tttcgtggat atgtagaaca cgtctataat cactcagcga tcattcgcat
     8521 tgaaaacacg atggaatgcg acaagttatt agcgaaaagc aaagataatt tagcagtggt
     8581 gagattggtg gatatggagg ttataaacaa tgaaatttaa aatctttgaa gaggacactc
     8641 gctataaatt agaaaaaaaa ttaaatgaat ttgcaaagaa taatgaaatt cagcatatat
     8701 ctttaacggc ttctaagtca ggttatacaa cttactatgc agctgttgtg agctacgtaa
     8761 ttcaataaaa ggaactcggc aaataaaaaa gccggatcac tccgactgat gtaataaatc
     8821 cgacaagttt attatatcac ataaaggagc ggtttgactt gatgcaattg ttacgagagg
     8881 tagatttcaa acagacaaga tgtaatgcga gagatgtgct gaagaacttt cggcgcttgg
     8941 agcggatggc aggtcgctct ttgatagata ttaagtcgcc gattattacg gatatgccga
     9001 aggcaccgaa gcacggcaat aaggcagaag acgcgatcat tcagatgatg gatatagaag
     9061 cggagagaga cgcgatttta gcggctttga tggcgcttag tctgattagc cgtcagatac
     9121 tctactacag cttctgtgac gtaaacaagc actctaatta tgaaataggg caattgatac
     9181 gaggatacgg agagaagaat gtagagaagc tgaaatccat cgcattgatc gaatttgctg
     9241 aagcatacaa aaaaggcgtg ttagttcagt atcgttaatt ttgtagggtt tttgtaggga
     9301 tagtgtaggg tttttgagcg gtttaacgtg atattatgat agtgtcgaaa gattagtgat
     9361 aggtctaaga caaaataata ataaaaggaa catcgtttta ttattgtttc acaattaagc
     9421 ttcgatagac agcagcggaa atattaagaa taaggatgtg aattttaaca gaattaggca
     9481 gaagtcgccc atcctctata ggtgggtgat gaatgccgtt cggtatgagg gttaccgttg
     9541 gtaactcgaa gaccgacaac taaggcgtat tgccttttta ttttgt
//
LOCUS       DACDMM010000101         9569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDMM010000101 DACDMM010000000
VERSION     DACDMM010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.102"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(140..877)
                     /locus_tag="IWD16_001956"
     CDS             complement(140..877)
                     /locus_tag="IWD16_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9088966.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(944..1513)
                     /locus_tag="IWD16_001957"
     CDS             complement(944..1513)
                     /locus_tag="IWD16_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP9088967.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1641..2513
                     /locus_tag="IWD16_001958"
     CDS             1641..2513
                     /locus_tag="IWD16_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9088968.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            2855..3727
                     /locus_tag="IWD16_001959"
     CDS             2855..3727
                     /locus_tag="IWD16_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9088969.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            3724..5682
                     /locus_tag="IWD16_001960"
     CDS             3724..5682
                     /locus_tag="IWD16_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9088970.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(6014..6865)
                     /locus_tag="IWD16_001961"
     CDS             complement(6014..6865)
                     /locus_tag="IWD16_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9088971.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            6988..7455
                     /locus_tag="IWD16_001962"
     CDS             6988..7455
                     /locus_tag="IWD16_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP9088972.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(7718..8200)
                     /locus_tag="IWD16_001963"
     CDS             complement(7718..8200)
                     /locus_tag="IWD16_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9088973.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(8300..9163)
                     /locus_tag="IWD16_001964"
     CDS             complement(8300..9163)
                     /locus_tag="IWD16_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9088974.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         2817 a         1973 c         1743 g         3036 t
ORIGIN      
        1 tgtttctttt taataaattt gctttttctg cataaaaaga cgtaaatgaa gaatattctt
       61 ctccattcac gccttaaatt gatggtcgtt tgaataacta tttaacaagt aattctctct
      121 ggatattatc cgttatggat tatacttcct gtgtcgtcgg acggataatc atgttgctga
      181 cagacatccg gtctggcgtt tctatcgcaa atgctacagc agaagctata tcttcagatg
      241 ttaatcccca ttctttttgc gcttcatgaa gttcaagagc agcctttttg tcgctgatgg
      301 tttgatataa ttcagtttga acagctcctg gagagatgat cgtagatttg atgttatttt
      361 gccgttgctc ttgacgtaat ccttccatga ttgcacggac agcaaatttt gttccgcaat
      421 aaacggcgga atcaggataa acaacatgtc ccgccacaga atcagttgaa atgatatgcc
      481 cagatttttg tttttccatg attggaagta cggcggaaat tccatttaac acacccatga
      541 tgttgatatc aagcatattt tgccattcat cacggtgacc ttcaactaat ggagcggttg
      601 gcatgattcc tgcattattg tacaatacat cgattcgacc gaacttttcc atcgctaaat
      661 cgataactgc ttggacttgt gcaaaatctc ttacatctgc tgtttgaata tatagctcag
      721 cttctggtaa agattctttt attgctttta ggcgatcttc tcggcgtgca gctataacta
      781 gtttagcccc tttttctgct aatagcttcg ttgttgcttc accgattccg cttgaagcgc
      841 ccatgatgac aatcactttt tctgataaat cattcattct attctcctcc tgttttgaaa
      901 aatctcttgc ttatactcac tatctcttat ttcatcttac tgttcaaata atccgttcga
      961 caggtttgac gattttggca gggattcctg caacgatggt attgtctggg acatctttcg
     1021 taacagttgc attcgctgca acaatggcat tctcgccaat cgttacgcct ggcagaatag
     1081 ttgctcctgc tccgatccaa gcatttttct tgatgtggat tggctttact gacaagcctc
     1141 ttcgttcttt tggatcggtt agatggttca ccgtgatgag gttgacacga ggaccaatca
     1201 atactgaatc ttctaaacag atgccaccta agtcaacgaa catcacttga cgattgataa
     1261 agacattttt ccctatacta atatgtttgc cgaaatctgt ataaaaaggc agcagaacag
     1321 tcaccgtatc atctactttg tcactaatga tattttctag ccgtttgcgg atatctgctg
     1381 gttcatggta cccagtattc agcgctgcta aaggctcttc gttttccgct tggatctgat
     1441 ggatcacttc aaagagcgga tccgttactc gaatctcttt attgacgatt tgtttttcga
     1501 tgaggcttgt cattttgctc cctcctttta tccttacttt ataacggacg gcttatgatg
     1561 tctaatactt ataaaacata cttaactatt ccttttaact atacttagtg gtatgctttt
     1621 ttaaaaacag gagggaaaaa atggatattc gtatactgac gtatttttta gcagtagcta
     1681 gagaaaaaaa tatcagccga gcagcaaaat ctctgcatat cacgcaacct actctctcaa
     1741 aacaactgaa agatttagaa gaagaacttg gtgtgacact tttcactcga ggctcccgag
     1801 agatacagtt gacggattct ggtcgatatc tttattcaaa aggaaaagaa atcttgtcgt
     1861 tggtagaaaa aacggcgaac aatttaacaa atgacgcaat catctctgga gaaatctaca
     1921 ttggcggagg cgaaactaaa gcaatctctt tcatttctga tagccttcat tcgttgatca
     1981 aggcgcatcc tgatatccag tttcatttat atagcgggaa tgcagatgat atcatagaca
     2041 aactggatag cggattactt gattttggac tcgtaatcga accgacaaac aaacaaaaat
     2101 accagtcatt tcgactgcca gcccaagatc gttggggact tttgatacat cggaatcatc
     2161 ctttagcaaa gcaaacagtc atccatccgc aggaactgaa aactgttcct ttgatcattt
     2221 cccggcaaac gtcagtggac agtcaactag ctgaatggct aggtttcagc atagatcagt
     2281 tatcgatcgt cggaacatat aacttattgt acaatgcttc tttgatggtc gataacggga
     2341 cttttggtgc attagctcta gatgggatca tcaatactta tgggacggaa aaagtattca
     2401 tcccactcga tccgccatta caagcttcac tgaatctcat ttggaaaaaa gaccagcctc
     2461 tttctcctgc tgctcgtaaa tttttagatg tcctaacgga gaaatacagt taagtagagt
     2521 ttttttcctt cgctttcagc tttttaacaa cgaataagtt aagcaaaaga tccaatgatt
     2581 aaataacata agtttcttaa tttgttcctt ccttttattt ctgataattc cagtaaatta
     2641 ctataattat aaaaaaagaa ttgacaaaac ataactgtct atataaaatt gattacgtta
     2701 acaataattc gtttcatgtg actagatcat actaggcatg aaaaatctct gaggaacatg
     2761 gtttccctag gaggtttttc gtgtctattt ttgtttggcc gcaatcaact ggtctagcaa
     2821 acgtgaaaac caaatcttca aaaagggtgc gcgcatgaaa atcaaaaaga tctttaatca
     2881 aaatgcagta ttggtagatg acggtggaca ggaaaaagta gctattggaa aagggattgg
     2941 atttgacaga aaaagaaatg atttgatttt tgatcgtgat gtcgaacgga tatttgtcat
     3001 ggaaccagaa ggtcaaatca agttgcaaaa tttacttaat cagattgatg aacgcttttt
     3061 atttgcagca gaaacgatca ttgatcatgc ggaaactgtt ttgatggaaa agttgaatga
     3121 acatgtattg atcgctttga ccgaccacat tgctttttct gcggagaaca ttaataacgg
     3181 tatcgtcatc cgcaacaaac ttttaagaga aatcgaagtg ctttacagtg atgaattcag
     3241 tattgctcaa tgggcagtag agtatctgac aaaagagctt gggattccat atacttatga
     3301 cgaagcagga tatatcgcaa tccatattca cagcggacga agcggtcgga acaataacca
     3361 tcgcagtatc cgtgaagtga cgattgtatc tgacatcatc catttagtgg aatcagaatt
     3421 ggaaacagat atccattcag aaccattttc actgaattat tcaagactgg tgaatcattt
     3481 acgactgctt ctacaacgaa cccatgcgca acaatatgct gttttagaca cggaaatcgt
     3541 agaaatggtc aaacgaaaat acccagaaag ctataagatt tctaaaaaaa tccgtgtgtt
     3601 gctgaccaaa gaataccagc tcgcgatcac gaaagaagaa ctgggttact tggccatcca
     3661 tatcgagcgt ttaagaagcg caatcgtaca aacaaatgtt aacaatcatg aggaggaaaa
     3721 aaattgaaag catatctaca acgaatggga cgttcccttc aacttccagt tgcggtctta
     3781 cctgctgctg ctttactagt agggatcgga aactggtggg catcttatag taacgacatt
     3841 gtcgctcatt ttttacaagc tggcggtaac gcagttttag gtcaattacc tttgctgttt
     3901 gccgttgggt tagcactagg gatgtcagtg gacaaatcag gtgctgctgc cttggcaggt
     3961 ttggtcgcat ttgaagttcc agtaaatgtt ttaaaaactg attcagtagc tactttattg
     4021 aatatcaaac cagaatcagt tgatccatct tttgctcaga tcagtaacgt atttattggt
     4081 attattgccg gattgattgc ggcagctctt tataatcgtt tccacgaagc aaaattaccg
     4141 atggctttat ctttctttag cggcaagcgt ctagtgccaa tcttggctgc ctttgtcatg
     4201 ttgctgatca gtgcagtgct actgctcgta tggcctccgg tttactctgc tttagtttct
     4261 tttgctaaat tcattttgag tttaggctgg ggtggtgcag gtctttatgg cttcttcaac
     4321 cgtttactga tccctacagg attgcatcaa gcaatgaact ccgtgttctg gtttgacgta
     4381 gcaggaatca atgatattgg taaatttttg gcttctgaag gaacaaaagg cgtaacagga
     4441 atgtatcaag ctggattttt cccaatcatg atgttcgggc ttccagcagg agcatatgct
     4501 atttacagaa atgcacgtcc agaaaacaaa gcaaaaacag cttccttgat gttagcagca
     4561 gcttttgctt cattctttac tggtgtaaca gaaccacttg aattctcatt tatgttcgtt
     4621 gcttggccat tatatgtctt gcatgcaatt ttcactggga tttctttagc agtgagtgca
     4681 ttcttccatt ggacagcagg atttgccttt agtgccggat ttgtcgatta cttcctatct
     4741 ttgaaaaatc cgattgcgaa tcagccattg atgttgatcg tccaaggatt agtaacagca
     4801 gtgatttact actttggctt taactttgct atcaaaaaat tccatctgat gactcctggc
     4861 cgcgaggaag cagatctttc agatgacgat actgcaacga ctaattcaga caataaatat
     4921 gcggcacaag caagcaaaat ctacgctgca ttaggcggag ccgacaatgt aacttcgatc
     4981 gataactgta ctacacgcct tcgtttgcaa gtcaaagata caggcctgat cgatcaaaat
     5041 aaaatcaaag ccactggcgt acctggtatg aagatcatcg acggaaaaaa tgcgcaagtc
     5101 atcgtaggta cagaagtaca attcgttgca gatgagatgg caaaactaca tggtggtgct
     5161 gcaacacgac ctgcacaaac aaacaccgtg gttaaaacag aaacatttgc cggtggaacg
     5221 atcgaacaag atatttacgc tatcgcaaat gggaaactga ttccaatcac tgaagtttca
     5281 gatgatgtat tcgcggaaaa aatgatgggt gacggctatg ctgtattacc agaaaatgga
     5341 gaaatcttct ctccaatcgc tggtacgatc acaaacattt tcccaacgaa acatgctgtc
     5401 ggaatccaaa cagatgctgg tatcgaagtc ttgcttcata tgggcatcaa cactgttgac
     5461 ttaaaaggcg aaccattcac tttatatgta aaagaaggtc aaaaagtcgc aagaggtcaa
     5521 ttgattgctt tagttgatct tgccgctatt caatctgcag gcaaaaacac ggatatggtc
     5581 gttgtattca caaatggcga caaagtccaa agcttagaaa tcgaacccgc acgtgatgta
     5641 aaagcaaatg ataaaatcgg ctctgtttcc aacaaagcct gagccatcgt cttccccaga
     5701 cttttttatc gataaacaaa caggagctga gacaaaaatc ttgtctcagc tcctttttcc
     5761 gaataaaaac ggcgttctcc tgccaactcc gcggtaataa gtccagccaa acgtaacata
     5821 tgaagagcta ttttttattt gactcttctt attacttgat gcagaacagc ttttttacaa
     5881 cctctgataa acggtgttca aaaaccagtt tatcggtatg aagtcaaatt tatgcaaaaa
     5941 tgtggaaagc cattttcaca aaatttttct tcatactcaa aactaatcgg ctttttcaca
     6001 acctctttat acattattcc tgatccaata tttcttttaa tgctgcttca agagaagtta
     6061 atggttttcc caatactttt tctaaatcat cagaggaaat agctaactga tcctttttaa
     6121 tatcatgttg gatcgctaaa aacatgtcag ctgcttcttg agggaatccg gcttctttta
     6181 gattttcgac aaacgctgca tcacttgctt caataatttc taacttttta cctgtcgcac
     6241 gctctaatgc cttggctaat tcttcatagt cagttagatt gccggataat tctaagattt
     6301 ctggagagtc actttctact agtacttttg ctgcagcttc tgcatattca cgtttcaatg
     6361 cccaacctgt ttttccattt cctgcagcat aaacgaattg accagttttt aaagcctgcc
     6421 caatgatagg taattcattc tctacatacc aattattccg taaaaatgta tgggaaatac
     6481 ctgaatcctt aatcattttt tcagtgaatt ggtgatctgg cgctaataca ctgatcgagt
     6541 gatcggcatc tgcaaaactt gtataagcga tataagatac cccagctgct tgtgctgcct
     6601 tcactacatt cttatgttcg gcttgacgat ttcctggaac gccagagaca aaaagcaatc
     6661 gatctattcc tttcaacgct tctttcattg agtctaaatc atcataatca ccaatacgta
     6721 tattaaatcc agcttctttc agtgcttctc ctttttcttc actacgtact agtacataga
     6781 tttcattttg tggaaccatc ttttttaaga attccagcgc ataaccacca aaacctccag
     6841 ttgctcctgt tactaaataa tgcatatttg ccactcccta ttctttttat ttgttaagat
     6901 ttacagctgt acatttataa attacacctt tatcataaag agtgtggtaa gataagtcaa
     6961 gcaaaaaaaa tgaaaggatg acaactgatg aagtattctc tgcaatttag tgatgcaatc
     7021 catgtacttg cctatattga aatgtttcaa gatacccgat cactttctag tgagatgatt
     7081 gccagtagta tcgaaacaaa tgcagcaaat gtacgtaaaa tcatgagtca gctcaaaaaa
     7141 tccggtttga tctttacaaa aaccggaaaa ccgcagcctt cccttgccaa aagtccagat
     7201 cagatttctt tactagatgt ctatcgcagc atcgaaggta atacacagct tatccaagtc
     7261 gatccaaaaa caaatccaaa ttgcgtcgtt ggtgcgaata tccaagaagc tttagaagaa
     7321 agctacgctc ggctgcagaa aaaggcagaa gaagaaatgg ctaagatcac acttgatagt
     7381 ctgatacata aaatagccgt tttggaaaaa gaaaaaagac cagaaaacac aggcctcgta
     7441 gagagatttt tataattctc tctactgtgc ctgcattttt ctgatcggaa tcgtctacat
     7501 aaattctttt attctccggg ggacaaaagc cttgtcatgc ccctttttcc tctctaatgc
     7561 tcaggtcaaa gcgctgaagt catgacgtct tgataaacgg tgttcaagta gctgaccctc
     7621 ggcattagtt ctgaaaaatg caaaatatgg aaagctattt cccatttttc ctctctaatg
     7681 ctcaggtcaa agcgctactt tcacgacctc tatattatca tggtttattg actaccggta
     7741 tccagatttc tcccatatat ttcccgttgg taactcccat ttcgactaca gcatttggac
     7801 ccccaacata ggcgtaatct tctacttctc ttaaaacatg cccaaaggcc gtattcgtca
     7861 aatcatttct taattcttca ggcgattctg ctaaagcgat tacaacaaga tacgttcctt
     7921 ttgggaatgg gatgattcgg gtagtgtttg gaacatcctg actcgtttga acaccgacat
     7981 agtacatcat tttcccgtta tagggttcgt tgactgtaaa gaaataatga ttttcagcag
     8041 cttgtttcaa tgcatctatt gttccattac cgataacttt ttgctcaaat gcttgtttct
     8101 ctgcagccaa accgacaaaa tccgtattgc tactcttcaa ttccacacca atacccgtca
     8161 ccgtaaaatc tggtttttct tcgatcctat actttgccat ttctcccacc ctttctatta
     8221 tattaagttc atcttacata aaattaaagg gaaatgacac gattacgctc ttttttctag
     8281 ggatattttt attttttaac tatttttttg ctttcactgc aatccaaatc tcactgtaaa
     8341 cattactgaa atcctctccc cattttgtaa aagagatctc gggagcttcg actaactcat
     8401 aatctgaaga aggaagccat tctgcataga ttttccccca agtatcttgt aaagtttggg
     8461 gaaatggccc tttattagga aaaatggccc aagtgtgttc ttctatagca agcatttcta
     8521 aatcagcgta cggattttct tttgtcgtag caaagccaat catgtgcatc aaactgccct
     8581 tttcttccaa gcgggctcca tcaaatccat aagaaacgtt gattacttga tttggatata
     8641 aatctcctaa cttatgcatc tcttcccttt gagtctccgt gatcgtctgc gctaactttt
     8701 gtatttcttg attgactcct tcaaatttga caggaacttt tttcgccaca cctgccacat
     8761 aaaactgttc tttcttttcg atacgaaatt ccatcgattt tcctcctctg acatcaataa
     8821 aaaatgtcaa gcgtggaaaa gatttttgta tgccgtatct gctgatttct gaaggcagat
     8881 atcctgacca atcacgaaaa gctcttgaaa aaccttcgac tgtttcatat ccatatctaa
     8941 acgccgtttc tgtcactctt gatccatcta ggagatcgcg attagccaaa gccaatctac
     9001 ggtttttgat atagtccatt agcgacatcc ctgcaataaa cgtaaagatg cgttttaatt
     9061 gatattcaga gattcctgta ttttcggcta ttattttata ggaaagtcct tctgtcaaat
     9121 gttcttcgat gtaatcgatc aattggttca gatctttaag catatttatc ttcctccttg
     9181 cttgcctttt tatcatatta tcaaagaaag tcaattactt gatgaatcag gaaccaattt
     9241 agtcagatgt agaaaatgtt gtatggatct aaagaaaatt ctcaagcagc gtttcgtaac
     9301 ggataattgg tattatctgc ctatctatgt tttaccttta gaaaaataaa ttttggggaa
     9361 gacacattct aagtaaacaa agcggtaaaa atatctttta ttcccttagt taatccgctt
     9421 actacctact gtacaaagca aattgttgtt caaactgctg atcagaacgc tgcttggatt
     9481 tttctttgcg atacatcata cctcttttat caattggaac aattatttat ttctaacaaa
     9541 atcacttcat ttttgtcaga taaataaaa
//
LOCUS       DACDMM010000102         9484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDMM010000102 DACDMM010000000
VERSION     DACDMM010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.103"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(98..520)
                     /locus_tag="IWD16_001965"
     CDS             complement(98..520)
                     /locus_tag="IWD16_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9088975.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(510..2684)
                     /locus_tag="IWD16_001966"
     CDS             complement(510..2684)
                     /locus_tag="IWD16_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP9088976.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2700..5042)
                     /locus_tag="IWD16_001967"
     CDS             complement(2700..5042)
                     /locus_tag="IWD16_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088977.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(5064..7607)
                     /locus_tag="IWD16_001968"
     CDS             complement(5064..7607)
                     /locus_tag="IWD16_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAP9088978.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7617..8015)
                     /locus_tag="IWD16_001969"
     CDS             complement(7617..8015)
                     /locus_tag="IWD16_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9088979.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8096..8380)
                     /locus_tag="IWD16_001970"
     CDS             complement(8096..8380)
                     /locus_tag="IWD16_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088980.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8404..9402)
                     /locus_tag="IWD16_001971"
     CDS             complement(8404..9402)
                     /locus_tag="IWD16_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9088981.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2712 a         2019 c         1477 g         3276 t
ORIGIN      
        1 agtgaatttg tttcaaaaga aaaacaaata cgatcgaaaa aaatttgaac tcataacaat
       61 tttttgtttt tttcaacgta ttctctttca attttcacta aaaattagga agaaattgat
      121 tcaatgtctt ttcaaaaaag aatgtttttc tctcaatttt tttgacgttt ctatcatgaa
      181 tcacgagaaa acttgggaca cttttaacat ggtaacgttg taaaaaagtt tgaagttctt
      241 cttgtcttgg ttcttttctt gtttcaattc gatatacatt cctagagaat tttttgtcgt
      301 atctttccaa gaaagattca acttcttggc attttttaca atcatttctt gctagataca
      361 caaaaaatga ttgattcgtt ttttgaagtt ctagtacttc ttctaaatta ctattgatta
      421 gttttccatt tgattcttcg ttagttttta tcagtttaaa acaaccaaat aagaggaaac
      481 taacaataag tatcctaaca ataactttct tatttcgcat attgtttctc caaatttggt
      541 ttccgaatat agacgactcg cggtggttcc tggacgtctt cagctgttcg atacaccaca
      601 aattcgcctg ttctcaatgc tcgaatgcga ttcggattga atttaaagcg gtctacgtct
      661 cgtttcgtgc ctctttctcc tgtccaatca acctttccca tatcagaata acctgcttct
      721 tgtgtcgtca tttccgttaa ttcaatgtct tcttctgtcc ccattaaatc tgcccaataa
      781 tcaatttctg tcgggtcatt tgtcttccca ataaagaacg tattcacatt cccaatcaac
      841 tgttttcgaa ggacaggatc aattctatca atgtccgcaa gcgtttgagg ggcatacgcc
      901 gcatgcaccc cagcgcctcg tcccttattg actaagtcta taaggttatc atttagataa
      961 gaagctggtt cgtcaaatat tactaggact ttcgagcgat tggctgtttc tcctgcttgt
     1021 ttatacactt ctgacgcaaa gaaattaata tctcccacaa gcatttgagc caatgtccga
     1081 ataaactcac tataaatcaa tccgtttaac gagatataaa caatctttcc ttctttaaca
     1141 acttcttgaa tatctaattc tctttttcct ccagaagtat caaagagttc accaagctca
     1201 ctatccaata acatattgat attggtctgt aaattctcag aagtcgcata caaataaaac
     1261 gggttcttct tatgttcgta acgaacaaac aaccgtttaa acaacgtttg ctcgatttct
     1321 gtcatgagag aaatgttttc ttttaataat agataatacg tttctaagtc aacggtttta
     1381 cgatcatgtt caacgattcg ctcttcactt aactgttcca catcggcaaa atcacgttct
     1441 gttttgtcca tcgcttcatt ctttttcgtt tttttcttcg tggttttctt cttttctacg
     1501 accacttttt ctttaaataa atctggattt ttcaactgaa tacgatccgc aaacaaatgt
     1561 aagacgtttc gtggcaataa ataatgttgt aaccaaacta gatttctttc aattttatgg
     1621 ctcttttgga actcgtctaa aagctgaatc gttacctgta ataacgcttt ggctgctgtc
     1681 gaataataca cgctttctgt ttctgctaga ttggttaatt tatctcgaat ggcaatatca
     1741 ttgccatttt ttacaggatt ataacgcaaa tcgccaccgt ccacaaagac ttcaatcggg
     1801 cgattgtatg cctctgccac ttcttttaac gcttgtaaag tctctcctgc tcctttccca
     1861 tcgactaaga taacggggat acgaaactta cacgcatgtt ctgctagtaa ttggatcacc
     1921 gtcgtttttc ctgaacctgt agtcgctgga ataatcacat gttggttcaa ttcactaaag
     1981 ttcatcacat aagggagccc atactcatct gttcctaata gaatcacttt ggctaatgaa
     2041 gccagttgtt tattccgcaa agcaacgtct tttatgacaa ttgctacctg gtacgcttgc
     2101 tgatactctg ctgtatattt ttgggactcc tgaaatttat tcttccgacg ttccttgtat
     2161 tctttactat ttagcaaagc tgtttgccgt tctttctttg aaacaacttt tcttttttca
     2221 ataaaagacg catacgcaat caaacaactt cctgcacctg tcccacctaa aaggagggca
     2281 aaaatcgttt gtccattaaa ctgcaacgca tgagaaaaca agtttaatgg aaataataag
     2341 taacacgaca aataccctct aggaagaaca aaaataaaac aaaatagatt taatagcgta
     2401 aaacctaacg caaaaataaa aaaaagagag cttcttcgtt ttaagaagct ctcatactca
     2461 aagtcataaa tatgtgcacg tgtctctttt ttatctatca taaagacaat cgctaaagga
     2521 atactgccta caataaaaaa tggatataga caaacaccta ataaagtgac gataaaataa
     2581 agttgttgaa accgactttt tggcgtttct ttttcttgtt ttcttttgcg atcgacagaa
     2641 aacatttgct caaaatgtgt cggtcgtttt ggtgcattca tcatactatc gatttcctcc
     2701 tatcgttttc gttgtttcat ttctcttcga taaatcggtt gtttttctcg ttttcgtttc
     2761 atttttgctt gagttgctcg aaactcactc cgtgtcttcg gtgcttgttt tggtgatttt
     2821 gtttgtttca tggttgagta tgctttagat acggttgaag gcgctttggt ttgtgctgtt
     2881 actcgattga ctgctgacat atgttcccga accccctctg gacgtcgaac agatttcggt
     2941 gtggtagaac ctgttgctcg tggtacattc ccttttctta gtggtttttg taccgcatgt
     3001 ttttgtcctg caactgtccg ggttttcatc gaacttgttt ttgttacggg agtaagtgcc
     3061 tccttccgac gacgacttgt ttgtccctga cgacgttttc gatcgttcaa ttctgttcta
     3121 ggcgtatgct tctggccata agacttgtgt ttttgctttg gattcgctaa tgacaccacc
     3181 attgcagaac cctgattgcg atacggacta cgagacgcac cttttctact tctttctctt
     3241 agactaggat tgccttgcaa ttctcgttgt aataaatcgg ctgtttttct tgcctgtttc
     3301 tccattgctg ttcgttgcat acgttcttga ctttgctctt ctgcttgttt cgcttgatca
     3361 tgcaagtctc ttgctttttg tgccatacgt tccatacgat ttggtgcttt atgacgaggt
     3421 tgttgtgcgg attcacgccg aaaagcactt cgtgtggctt gtttcgccgt ttgtggcata
     3481 cgaacgtctg agccaccccc acgttttcgt tgacgttctt gtctcttccg tgcgttttct
     3541 ttttcttttt gtttctgtgc ttttctcgct tctttgcgtt ctttcaaccg ttgtttggct
     3601 tcccttctca attttgcttc tgccccaaat ggatttcccg caaaagctaa cgctcctcga
     3661 acacccattc ttccagtaaa tagactgcct aaaaagcctc ggaacaaata aattcccaac
     3721 ggcgcaaaca aataaacaat gatcaagaga attttttccc atggatcgtc ttgtgtacta
     3781 cttaattgga acccgtaact aagaaaagaa gaaaaaagga tacatgcaaa acctgtcatt
     3841 ccttttaaaa acgtcaaaat accaaaaact ttggcatagt taccaaacac attcattgtg
     3901 acaagaaaca atcctaaaaa aagtaagaac ggcaacatcg gtactaacaa tagttgcata
     3961 gttccaataa tcaatcgaaa catacttaag acaaaataaa caacggtttg aatcagattc
     4021 acgacaaaat aaaacaaggc aaatgctgta tttttccaac tattgtaata ttgtatgttc
     4081 ttgtttttca gtgtatctgc ttcatactct gtgactttgt catgaatttt ctcccctatt
     4141 tttgtaccta aatcattgtc caataaaaga ttgattcgat catattcttt tccagcatat
     4201 ttgactttct tctcacgaat tgtgtctaca ttggttgtgc cataatttaa caacagatat
     4261 ggttcataaa cattactata aaagacccga gccgccatca atttgcctgc cgtttccact
     4321 tttttatccg ctgttacctc ttctgtttct tttgtttcat aatttgtttc ttcttcaggt
     4381 gttaacactg gattgacctt cacgaacgcc gtttccactg tagaatctaa agaaaaaaga
     4441 acatcaaaaa gattcttatt tgtcccagaa tcttttaata cgcttaaccc agtaaaaata
     4501 aggatcgtca taagaaagac acggagtgct tgtttcattc gagcctctcc agcatatttg
     4561 attgccatca taattgccga aaaactgatt ccaatagttc ctgcaatact taacatattg
     4621 tccgcaattt tttgtgttaa gttttgaatg ggttctcgaa tggcggaaat aatatctaat
     4681 tcaaagagaa attcaaccat tgaagcattg aattctccta acatttttac gccactccaa
     4741 acaaaatttt tcatcactcc tgcaacattg tacgatagct tttttttaag gttcattgac
     4801 tcatagtcat tggtcatcaa ttcaaacgaa ttctttttgt aatttttata caattgatcc
     4861 acatcagtcg ttacttcttt tacttctact ttatcgttgg cattctctcc ttcagaacga
     4921 tcggtttcag tgacacccgc accatcattc atttgatcgg cagaaacagt agccccttgt
     4981 agacagaaaa aaagaccccc taataaaagg agcgtccacc atttcagaga tttttttcgc
     5041 aaaaaaacca cctctttttt gttttatcgt tgtttttcat tttctaacat tgcttgaatt
     5101 ttttcatctt gattttcagt agaagaaaca gccaataacc aactatcaaa caacacatca
     5161 atggcaatcg gttgatttct tcccaaatga tcctggaata aacaggttcc tgtttgtaat
     5221 tgttccatca tttctaaatt ggctgaattc acttccatat caaagaagga caatagtttc
     5281 tctcgtgctt ctttttgttt cggacgaaac gcaaatttgt aagaaattaa ctctttttta
     5341 tcttcttgat cgtaatcttt aaacgcttgc acaacaaggt aaatatccgt attattcgca
     5401 cgtccttttc gcaagctatc atcaatcaaa tagcttcctt gtgcggtatc ttcataccct
     5461 tttgcctcgt cataaataat cgcagcgtct tcttctttgt ttgtagaaaa gatatacgta
     5521 aacttcataa tgacttccat aattgccatt cctgcaagtt gttcgctatt taatcgaccc
     5581 gaatcaatct ctttttgtgt cggaatgcgt attccttgtg tccctaacac gttgatttgt
     5641 gaatcaaaac gtatcggcgt actgtgctca ttcccaatga gaatctttcc taaaccaacg
     5701 ctatgcccta aaatcaattt agctagttct ttgtcacttt ttcgaatttc ttcgataact
     5761 tgacttaacg aatgcttacc tggcatggtg taaactacat cgatggcgtc ttggatcacc
     5821 gttttcttgt ggctcgctgt ttctcgattg gtatctactt ctcctaacat tcctaacaca
     5881 tttttagcgg tttgaattgc ctcttctttt ggtaaaaaca atagaggatc aagcatccct
     5941 cgacagcttt catctgaaga taacgaaata aaattaattc gttcatacat agccacaaac
     6001 tctggtatgt gtccgaattt ttcaagtgcc tttaaaaaga acttttgggt ttcatttttc
     6061 ggatcaatat aaagaatttt ttgacctaaa aacatcagcc ataaaaaaat atattttaca
     6121 aggaccgatt ttcctttacc tggtggacca ataatcaacg tatttccatt ggtatgttcc
     6181 gcgccttcaa tacttttctt tgttaaattg ggataatacc atactagttt actagatagc
     6241 gctaacgctt ctttcgcaga tttaaacttt tttgcaataa caatccgtcc taaaggaaat
     6301 cctttcttat ttcctacttc tttctttaag tccaagccat aatctgccac aaaacctgtc
     6361 gtagcgacct gctcgtaact acgaaaacgt tgcgtacttc caattaagca ttgattaaac
     6421 aacgttagct gatccgctac cgatcgataa aatttgaaat ctgtagactc aatcgcaaat
     6481 tcaatatctt tgactcgttc ttccaattct tcttttgaag aagcagatac aaccagcgtg
     6541 acggtagaat aacacaatcg tttttctttg cgcttcagct tatcttttac atccattaac
     6601 cgttctgtac caaaatttaa tacatcgtct gaatctaaaa tgccgtcaac ggtctcatat
     6661 tctttttctt gttcataaat ccgtccagcc attttattaa tttttcgttc atcagatttt
     6721 gaatgctcaa atcgaagtcg aatatgcgta tcaattggaa aagataagct atcctgaaga
     6781 ttttgaataa agctactccc atacatggct aaaggagcgt ccgtttgact taaaaacgtc
     6841 gaataatgcg tttgattcaa ctgttcaatc gcaatgtatc ctgcttcatt cgaaatgacg
     6901 ccctcgcaaa tttccattgg cgtaagctgt ctttcaggca atttttcttc tgatcgatgg
     6961 aaaaaataat aataaatttg tccaatctct tgggcagata agcgctctat cggcttataa
     7021 ttggacaatt ccgtatagag ctccttttct aatgtttcat acgttggtaa cacactactt
     7081 ataggaactc gttgtcccgt aatacgatag ataaactttt taaaaacatg cttaagcaat
     7141 tgaaaatatt cgatcggatc agacactttc atttctgtag tgaaacgcac aaacagatac
     7201 gttcgatact cgtttaattg cacttcgtct tttaaaatct ttcctgcctg ttcaaagtac
     7261 actttcccaa gatctgcgca ttctcccttt accaaatctc gaatggtttc ttcaatgtgc
     7321 gtatctaggt taaattgacg tggaatattg ataaaatgat agtcatattc atcatgtcca
     7381 agtatgcctt cgccatcttg aagatagttc ttgaaaaaat caatgtcatt taaaggaaat
     7441 acttgggaag aaagttgata gcctacccac acatcatcat ttttggtcaa aacaaaattt
     7501 cggataatgg cgtctatcgg aaattctaat cgaggaagct tcgttttttt ctttttgaac
     7561 tgccaaatcc cgataataaa gacgaccaaa agaaacaaaa acgccattta ctcgactcac
     7621 tccttccatt tgtgtaggga atacgtcact ttttcatgga aaaacggcac atacatttca
     7681 tgttcaaagc gttgtttccg tttctgataa taggacgttc gcccaattaa atactcgaaa
     7741 aagtgtttat tgtcccattt cattgaaaac aatgtccaaa cgagaacacc aatcccaaaa
     7801 atcataatca accaggactt tcgaaacagt gtctgcaaga aactaatttg attcacatac
     7861 gcaatcaaaa ataggatcaa aagaatgaag agaaataccc cacctatgac taattgttcc
     7921 atgcgaatgc cattagaaat ccagggaatt ttttgtcctt taatcccgta aattttgtga
     7981 gggtgcttaa actcctttgt atagttgtat tccatgttca ctctacctcc ttttacaaaa
     8041 aaagaagcga ccagaagttc taatcttctg atcgcttttg cagtttgtct ttttcttatg
     8101 ataattgtat ccagggcaca atcatttgcg ttacccccac gttctcttca ctatccttaa
     8161 taaagataat gttctttagc cattcaccaa tatcaacggc attcgataac aataagacga
     8221 gcattatcaa aataaagacg ccaataatgg caaacgctgc ccgcgcaaag ccttgggtcg
     8281 cataagcacc aaccattaga acaatcgcaa cgattgccaa tagccaaatg acttgtccag
     8341 atacatcatc aaataacgat ttaagatcaa aaagtttcat ttccattctc ctctaagttc
     8401 attttattca tttaacgtag taacaaaata actatcgcca cttgttttta cttccattga
     8461 aaagtttgat tggaactcgt ttcctttctc aaaatagaaa cgatacttcc cttgcaaaaa
     8521 gtagtttcct tctttatttt ttactgcatg atccagttgt acattgatca attcgccgcc
     8581 ttctaaccct ttttcgttag aaatcaatgc caatttttca tcatttttga catataactc
     8641 aaaaaaccgt ttcacaaatg cttccagctt gatttcttcg ctttcagtga ccgcttgccc
     8701 agaagaataa aatcgatttt cctgatagtt cacagaattg gtattcttac tctcagaaag
     8761 attcaaaaat tgaacgccat caagtacttt taaaacattg ttttgataac tgactggaaa
     8821 agaaagttga ctgcttttcc aatgttctcc ttctgtaaaa gtaacctcat aaaataggcg
     8881 atacattgct tgtgtcaaat cgatttcttc tagtttaata aagcgaacat ctttcgctac
     8941 tcgattggta gccgtcgtta caaagcctag atcagacgct tccaatcctt gtgctaaata
     9001 atgattgacg gaagcctccc attcgtttcg tttttctggc gaataagtaa ataacctttc
     9061 aataaaatgt tgtccctcgt acttcaattg ttccgtagaa gacaaggttt ggttgacttc
     9121 agttaacaat gtttctggat tgacttgttt tttcactgct aaacgcgtta atgtatccac
     9181 tcgtccaaag tttaggagga gcacgacacc cattaacaga aacaaacaaa aaaaagcgcc
     9241 tgaaaataca ctcatggcac ttttttgcga aaattggatc attcgatctg acggttctgt
     9301 tttctttttt tggaacaaaa aacgtttctt ttgtttcttt tccttctgtt gtttcggtgg
     9361 cttagtttgg aacgcttgtg gattaaattt tgcattactc atgagaaatg ccataccgtt
     9421 ctaacaataa tttcggtatt ttgtccacaa tcactacttc gtggacaacc acacttgccc
     9481 cctc
//
LOCUS       DACDMM010000103         9281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDMM010000103 DACDMM010000000
VERSION     DACDMM010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.104"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..435
                     /locus_tag="IWD16_001972"
     CDS             <1..435
                     /locus_tag="IWD16_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9088982.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="IWD16_001973"
     tRNA            complement(759..842)
                     /locus_tag="IWD16_001973"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="IWD16_001974"
     CDS             1370..1870
                     /locus_tag="IWD16_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9088983.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="IWD16_001975"
     CDS             complement(2060..2404)
                     /locus_tag="IWD16_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP9088984.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="IWD16_001976"
     CDS             complement(2558..3478)
                     /locus_tag="IWD16_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9088985.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="IWD16_001977"
     CDS             3665..4156
                     /locus_tag="IWD16_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP9088986.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="IWD16_001978"
     CDS             complement(4180..6555)
                     /locus_tag="IWD16_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP9088987.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="IWD16_001979"
     CDS             6881..7507
                     /locus_tag="IWD16_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9088988.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="IWD16_001980"
     CDS             7530..8234
                     /locus_tag="IWD16_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9088989.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="IWD16_001981"
     CDS             8426..9259
                     /locus_tag="IWD16_001981"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP9088990.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3011 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct t
//
LOCUS       DACDMM010000104         9113 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDMM010000104 DACDMM010000000
VERSION     DACDMM010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.105"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            5..613
                     /locus_tag="IWD16_001982"
     CDS             5..613
                     /locus_tag="IWD16_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP9088991.1"
                     /translation="MFLAACQSNTNQGTSGNTSSSSEQRSSQVSDSSTKASTSSSSQT
                     KSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYFYKIEGVDDQKEYE
                     ARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAAKIAVDKVGSGTAT
                     DWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            697..1641
                     /locus_tag="IWD16_001983"
     CDS             697..1641
                     /locus_tag="IWD16_001983"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP9088992.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1776..3227
                     /locus_tag="IWD16_001984"
     CDS             1776..3227
                     /locus_tag="IWD16_001984"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9088993.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3248..4084
                     /locus_tag="IWD16_001985"
     CDS             3248..4084
                     /locus_tag="IWD16_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9088994.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4084..4743
                     /locus_tag="IWD16_001986"
     CDS             4084..4743
                     /locus_tag="IWD16_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9088995.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            4869..5558
                     /locus_tag="IWD16_001987"
     CDS             4869..5558
                     /locus_tag="IWD16_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9088996.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5564..6520
                     /locus_tag="IWD16_001988"
     CDS             5564..6520
                     /locus_tag="IWD16_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP9088997.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6564..7076
                     /locus_tag="IWD16_001989"
     CDS             6564..7076
                     /locus_tag="IWD16_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9088998.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7332..7880)
                     /locus_tag="IWD16_001990"
     CDS             complement(7332..7880)
                     /locus_tag="IWD16_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9088999.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(7969..>9113)
                     /locus_tag="IWD16_001991"
     CDS             complement(7969..>9113)
                     /locus_tag="IWD16_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP9089000.1"
                     /translation="LGLAFLISSNKKAIKLKPLIVMFVLQLVLGFVLLRTTFGTAVVS
                     VLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISAIIGILRYIKILPL
                     FMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFLSEKRLFTMSVSAM
                     STVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEEKEDELIIEENKEQ
                     TFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGITFQEILGYIFAPLA
                     FISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTAIISVFLVSFANFS
                     SIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLLV"
BASE COUNT         3008 a         1676 c         1856 g         2573 t
ORIGIN      
        1 agctttgttt ttagctgctt gtcaatcaaa taccaatcaa ggcacatctg ggaatacgtc
       61 ttccagtagc gaacagcgct cttcacaggt atcggactcc tcaacaaaag caagcacatc
      121 ttcgtctagc cagacaaaaa gttctagtga agcaagtact cagtcagata tcaaagtgtc
      181 tgcagctcgt gcgatcgaat tgttccaaga taaataccca gatactgcaa ttactagttt
      241 ggaattagat agtgattggg gatcttactt ctacaagata gaaggtgtcg atgatcaaaa
      301 agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg gagacagaga atccagaaac
      361 tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa gatggtctgg atgttgataa
      421 attgatcacg attgaggaag cagcaaaaat cgccgtcgac aaagtaggaa gcggtacggc
      481 aacagattgg gatttggata aagatttagg tactacttat tgggaagtca aagtaaaaga
      541 cggcaatcaa agtacatcag tcaagatcaa tagccaaaca ggcgaagtac tgtcaactga
      601 acaagatgat taagaatttc ttcggaaaac aagagttgat ttttctttta gtcatgatac
      661 actagacaaa ataacttgtt tgaaggagcg atttacttga gtttagaaac gacctttgcc
      721 aatcatatct ttgccaatcc tttgatgaat gcatctggtg tccactgcat gactacccag
      781 gaattagatg aactggctca ttcagaagca ggagcattta tcactaagag ctgtacgatc
      841 aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg ttcctttagg gagtatcaat
      901 tctatgggac ttccaaactt aggtttttct tattatctag agtatgctct tgcgtatgaa
      961 aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca ttgccgggat gagtgtccag
     1021 gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt ttaatgggat cactgaatta
     1081 aatttatctt gtcccaatgt gcctggaaaa ccacaactag cttacgattt tgaggccact
     1141 tatgaaacac tgaaagaagt attcagcatc ttttctaagc cgctaggaat caaattgcct
     1201 ccttattttg attttgctca ttttgatcag atggctgata tcttgaatca gtttccattg
     1261 acctatgtca atgctatcaa cagtgtgggc aatggtttgt atatcgatac agaacaagaa
     1321 gcagtagtca tcaaaccaaa agaagggttc ggcggcattg gcggagagta tatcaagccg
     1381 actgctttgg cgaatgttcg cgctttttat acacgtttga aaccagaaat ccaaatcatt
     1441 ggtacaggtg ggattcggac aggacaagat gcatttgaac atctgctatg tggcgcaagc
     1501 atgttacaga ttggtacaga gcttcataaa gaagggccgg aaattttttc tagaatcata
     1561 aaagaattaa cgcagattat gtcagaaaaa ggatatacgt caattgacga atttaaaggc
     1621 aaattacgca ccatttcata gcaaagagac gaaggtcatg attgatcttc gtcttttttt
     1681 ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact cattgtatgt tagacgaact
     1741 tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa tgatgcaaca gaattagcaa
     1801 aagagcttaa agaaaaagaa tttagttttc aagaactagt tacggatata cagaaaaaaa
     1861 taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct tgaaccattc gagagtaatt
     1921 cccacgagct attgattccc cagtcagaaa aagggccatt ggctggtatc ccatttcctt
     1981 taaaaatgct cggtcaagaa aaaaagggat ggctagctac ttcaggatct cgtttatttg
     2041 aaacacaccg agcttctcat acatctaatt atgtgcggca agcagaagcg ataggtctag
     2101 ttccttttgg acaaaccaat gcgccagagt ttggtttcaa aaatattaca gacccagtca
     2161 tctatggccc agcaagaaac ccttggaacc ttgaatgtac accgggcgga tcaagtggag
     2221 gagctgctgc tgcagtttca tcaggaatcg ttcctttagc tggtgctagt gatggcggtg
     2281 gttctattag aatacccgct tcgttttgcg gattgattgg gctgaaacct tcgcgaggga
     2341 cgatgccagt tggtccatat gcttggagag gatggcaagg agctgctatt gatttcggct
     2401 tgacagtttc tatgagagac acggaggctt tattttatgg gatgagaagt atccattccg
     2461 gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca tccaagaaaa aaacggttga
     2521 agatagcttt ttgtgttgat tcaccgatcc actcacagat tagtgaagag gcagtacttg
     2581 ctgtaaaaaa agctgtagat ttccttggaa aagaagggca tgatttgaca gagattcctt
     2641 acccactaga tggtcgtaaa ctgatcacgt cttattatca gatgaatggc gcagaaacag
     2701 cagcaatgat ccgctcgatt gaaacaggca tccagcgagc tgtcagaaaa gaagaacttg
     2761 aacccatttc ttggactttg ttccaatatg gtcagaagct ctctgccgct acttacatcg
     2821 attctctgca tgtatgggat gaagcagcaa ttgtcatgga aaacctacat caagaatacg
     2881 atctattcct ttcaccaacg actgcattta ccgcccctaa aatcagcgaa gatttgcaaa
     2941 gtgatgcgat aagagcacag atggaacaca tcagtgattg ttctgaagta gaaggtttag
     3001 acgttgtttc ttccatgttt gaggagagct tgaagatcac accttataca cagttggcta
     3061 atctgactgg acaaccagca atcagcttgc ctactcattt gacaaaagaa aacatgcctc
     3121 taggaatcca atttatggct gccagaggaa gagaagattt gctatttcaa atcggctatc
     3181 tatttgaagc aaatcatcag ttccatttac caaatccatg gaaatagcag aaacaaggag
     3241 aagaagaatg aagaaaaaaa gattgtttgc tctaataact agtgcctttt ttattttaag
     3301 cgcttgcggt acagctgccg ctaatcagga agataacagc tggagtaatg tagaaaaaaa
     3361 gaaagaatta gtagtagcga cttctggcac actgtttccc gcatcttatt ataatgatca
     3421 aaatgaatta gttggttatg atgtagattt ggcaaaagag gtggctaaac ggttaaatgt
     3481 caaaatctcc tttaaagaat acaacgtgga tggacaaatc acttctgttt caaaaagaga
     3541 atcagatttt gctgcgaacg attttggtat cacaaaagaa agagaaaaaa aattctccct
     3601 ttcagagact ttcaaatatt catttgacag tatgatcgta cgaaaaaaag atgattcagg
     3661 aatccactca cttgaagatc ttaaagggaa aaaagcagct ggcgaaccca atactagcta
     3721 tatgcgttta gcggaaaaac acggagctaa attagtcaca tatgacaatg ccacgaatga
     3781 tcagtattta agcgatgtag caaatggtcg aacagatgtt attctaaatg attattatct
     3841 gcagaaaatg gccgtatctg ctttaccaga tatccctgta aaaatactgg agaacgttta
     3901 tttcaatgaa aacgaaagtg gttttttatt gaaagaagga aatgatgctc tgaaaaagaa
     3961 aatcgatggc gtattaaaag agatgaaaga agatgggacg acaaagaaaa tatctgaaaa
     4021 atattttcaa actgatgttt cagtcaaacc gaaagaaacg atggctgagt cggtgagtga
     4081 gtgatgtact ttcccctaat tgatttttcc ctcatgtgga cttcacttcc tttcgtactg
     4141 caaggcttgg tatacacatt agggattggc ttcgtgtcgt ttcttttagg aaatttggta
     4201 gggttgcttt tgacagtttt aggtcttttg gattggctgc ctctgaatgt atttattcgt
     4261 ttttatttat cgttctttag aggcattccc gcactagtcc tgctgtttct gttatatttc
     4321 gggttacctt atcaattaag tgccttaacg gcttcagtca tctgctttac gattaccagc
     4381 agtgcattta ttggtgaaat ctatcgagga tcactagcag gcgtttcttc aggtcagtgg
     4441 gacgctggtt tagcttctgg tttttctttc tttggggtat tgaaatatat cgttttgccg
     4501 caagcttttc gaatctcagt accggcactc agtaacgtag ctatggatct agtgaaaggt
     4561 acttcgttag cagcaacaat cacagtgcca gaaatatttc aaaaagcaaa gatcataggc
     4621 ggtcggacat tcgattattt ttctctctat attttagttg cactgattta ttgggtcatt
     4681 tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac gttctatcca tcagtcaatc
     4741 tgaaaaatag aaatggtgct agcgtggctg gcaccatttc tattttttgt ttcttgtata
     4801 ttctgcctag tattttaaaa ccatttcatg taaaatgtaa atcgaaaata tgttcggagg
     4861 tagaaaacat gaagaccccg ctaaccatcg aaatagaatc ctcgttatac caatattgca
     4921 tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc tgatgaccaa ggaattgtag
     4981 atacgcttag ctgcttgaca aaatttgatg gaagccgaga atggcgatgc tatgagctga
     5041 aagtgacaaa agcagatttt cgctctacag ccaaactgtc atttgttgga cattataatt
     5101 attttgtttt accaagtaaa ctatatgaag aagtaaaaac tgaaatccct acaggaatag
     5161 gtgttttagt gtatcgtccg tacacggttg aagaggagat gcctgcctct ggtacatttc
     5221 tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc ggaagaagct ttgacaaacc
     5281 gcttcatggc ttctttattc agggaagtaa gaaaagcaaa gcaaatggag cgaggcgtaa
     5341 aatattttcc aaaagatcaa ttgtacaaag aattgaaaag acgttcagaa aatcaagatc
     5401 cattttctaa cgaacgatat tatgaacggt ttttagagga aatagctagt caggcaatca
     5461 atgagttgca agaagaagtt atcgcgcttc agcatgatta tgaatatttg aggcaacaac
     5521 agcagatccg tcggttgcct acagagccat tggagtgaaa aatatgtact atccttattt
     5581 aagaggaaaa caatttgacc tattagcatt aaaagaagca ctgtcgcgag gattgctctc
     5641 caataaaatc caaccagtga tcgaaccagt acgtgattcc gctacgctta aaaatgtgat
     5701 cgaattattt caaaaaaagc accatccaat cgctgtgatc caaaatcccc aagtaggaca
     5761 attcaaatta ttcgatcagc atgtgcacag ttggacagtc aaagaaaata gctctgtcgt
     5821 ccctgcacag atcttaacgc cagaaaatct cagtgaagta ctggattcac ctccagcgtt
     5881 tcttgttttc gatggacagc actacccaaa agatacagag gtgtggaaac agttggcagg
     5941 agttgattcg aaatttttga taccagatac tagtcgcttt cgcatctggc tgcccgaaaa
     6001 caaaattgtg atccgtgact cttttcaaac gagaaagcat gttgaaagtt atgcagataa
     6061 aacagatgac ttttttagtg atgactatct ttttttccac gcagacggat atattggatt
     6121 ttctgatttc acgatcgaag gtagccgtta tttcgataaa gggttcccta gccgagcgct
     6181 tgctatccat ctaacgtata ttgatgctta cggcaatata cgcgtgaaac attttgtctc
     6241 tgacagtaac gactctgcta aagaccaagc attgaagttt cttgaagcag gagataaaat
     6301 gaaaaaatgg atcatgcgtc accatcatca actcttgatt acttcaggaa tgattgaatt
     6361 attggctctt taccagcaac aaaaattccc tggactggga gtgttgaaaa agtggtcact
     6421 tatgcatcat ttggaactta ttagtcacct gctagatcat ccaaaagatt ggcttaaata
     6481 ttattcaaaa gttcaggaac tatatgaagt gatccaatga ttagataatg taaatgaaaa
     6541 aggaatagaa aagaggaaac tggatgtata ttcggcaaac aaggaaagaa gaagtagaag
     6601 aagtggtaga aatcattaat gccgcagcgg atttcattgc ttcgcaaggc tctcctcaat
     6661 ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa cgatattgaa agaaaagaaa
     6721 gctatgtatt aatagtggaa gatcaaattg ctggaacagc tgcattagtc tcaggcgtgg
     6781 atcccgttta cacaaaaata gatggtagtt ggaaagaatc aactggtcct tatttatcga
     6841 ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa actagcaaaa ccgttcttag
     6901 aacatttgtt gacgattgca gccagcaaaa atatccaaga tatccgtatc gatacttatc
     6961 cagctaatga accgatggaa aagaccatct attctgctgg attcacttat caagggatga
     7021 ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca aataatcatc aattgactat
     7081 aaaatcatct gagcaaagat aaagacatta acactactga aaaaaacaat catatgatag
     7141 ccaagaggtt gtaacagaag tggccagctt caaaaaacaa gaaagaattt ccgaaaatgc
     7201 ttctcaaatt tttgtggaat tttagcgtat tttacgaaat agctgctttc tgaaactccc
     7261 ttcatcgaga ggtcgtgaca tagcttttgt cacgatctct cggcttatcc tacgagtatt
     7321 attcgataca tttatatcaa taatcctttt tttattttat ctgcagcttc ttggaacgct
     7381 ccatcgtttg acacgactaa aagtgagggg atagctttca aacgtttcaa ctgtgctaaa
     7441 tcttctacat aaaccgccag tctggctgaa atctgttcag atgaatctcc acgttttttc
     7501 aggcgcgatt gcaatgtttc tttagagatc gtaataaaga taggacggac taccgtcccg
     7561 aaatactcat ataaatgcca aaaaccattt gctgtcaaaa cggcgtaaca atttcctact
     7621 gtcagtttct ttttaatttc tgctttactg gaaccatatt tatgttgagc atattggtcc
     7681 cactctgcaa actcatttcc cttgatcatc tgattgaatg tattgtcaga aacaaaataa
     7741 taatcctctt gatctttttc cccaggtctt ggtgatcgat tcgtgtaagt aatgatcttt
     7801 tgttccggct gaaaaatagc ttctgcgagt gaagtttttc cagaaccaga aggaccgata
     7861 aatacatagc aataattcat tgtcatttct cctttgatga ttgaacaagt gaaaaagctt
     7921 tggaaaatat ttccaaagct tttaaaagta actttgcttt tttgtatttt ataccagcaa
     7981 gccagtcaca atagcactta aaaagctgac taaagtcgct gttagcaaaa ttttcaatcc
     8041 gtgttttgcg acaagatttc ctttttcttc atttaatccc ttcatcgcac cagaaatgat
     8101 cccgatagaa gagaagttgg caaatgaaac gaggaagaca gatataattg ctgttgtacg
     8161 ctcagtaaaa tggaaattac catttgctaa ttctgtcata gcaacaaatt catttgtaac
     8221 cagtttagtc gccatgatac taccggcatc cactgcttct ttccatggga ttccgctgat
     8281 gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg aaagtgatgc cgaatatccc
     8341 gtggaagata gcattgatca tggcaatcaa agcaacaaaa ccgataagca tagcagcaac
     8401 cgtgatcgcc acatggaaac catcaagaat gtattctccc aacatttgga aaaatgtctg
     8461 ttctttgttt tcttcaatga tcagctcatc ttctttttct tctaatacat agggatttac
     8521 aattgaagcc agaatataac cgccaaataa gttcaaaaca agggcagtga tcacatattt
     8581 agaatcgatc agtgccatat aagatccgac gatcgacata gaaactgtag acatagccga
     8641 tacactcatg gtgaacaaac gtttctcaga taggaagggt aattcctttt ttatggaaat
     8701 aaaaacttcc gattgtccta aaatagcaga agctactcca ttataagatt ccaatttgcc
     8761 catcccgttg attttactta atcctagacc aacagctttc ataaataatg gaagaatctt
     8821 gatatagcgt agaataccga taatagcaga aataaataca attggcatca atacggaaag
     8881 aaagaaaggt gcacttcctt tatttgctac accggcaaaa acaaagtcaa caccttgccc
     8941 agcaaaagca agaagatgat caaacatttt tgccaataca gaaacaaccg ctgtaccaaa
     9001 agttgtacgt aataatacga aaccaagaac aagctgtaaa acgaacatca caattagtgg
     9061 tttcaatttg attgcttttt tgttactgct gatcaaaaaa gcaagcccta atg
//
LOCUS       DACDMM010000105         9001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDMM010000105 DACDMM010000000
VERSION     DACDMM010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.106"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..594
                     /locus_tag="IWD16_001992"
     CDS             <1..594
                     /locus_tag="IWD16_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089001.1"
                     /translation="IFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNF
                     NQKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVN
                     LPYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKG
                     VADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            769..1602
                     /gene="msrA"
                     /locus_tag="IWD16_001993"
     CDS             769..1602
                     /gene="msrA"
                     /locus_tag="IWD16_001993"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9089002.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1682..2671
                     /locus_tag="IWD16_001994"
     CDS             1682..2671
                     /locus_tag="IWD16_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP9089003.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            2960..3658
                     /locus_tag="IWD16_001995"
     CDS             2960..3658
                     /locus_tag="IWD16_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP9089004.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            3808..5124
                     /locus_tag="IWD16_001996"
     CDS             3808..5124
                     /locus_tag="IWD16_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9089005.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            5141..6556
                     /locus_tag="IWD16_001997"
     CDS             5141..6556
                     /locus_tag="IWD16_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9089006.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            7020..7340
                     /locus_tag="IWD16_001998"
     CDS             7020..7340
                     /locus_tag="IWD16_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9089007.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            7366..7683
                     /locus_tag="IWD16_001999"
     CDS             7366..7683
                     /locus_tag="IWD16_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9089008.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            7712..8965
                     /locus_tag="IWD16_002000"
     CDS             7712..8965
                     /locus_tag="IWD16_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9089009.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         2982 a         1451 c         1876 g         2692 t
ORIGIN      
        1 atattctctt ctctgatatt aacgatgata tttttcaaaa atagaagata caataagaca
       61 agagaactat tttatttatt tatgactgtt ttgttattaa tcactttaac tgtttctagt
      121 aaaaaaaatt tcaatcaaaa aataatttct cagccgggac atattgttgt aaacaaagaa
      181 acagtagagg cgtataccac taaaggaatg gattattttt tatatgatta ttctcctatc
      241 gcttcattgg agaataagga gcatataata aaacacgaag caaagattgg cagtcaatgg
      301 gtaaaccttc cttataaaac aactggtagt tctatcatat atagagtaac taatgagaag
      361 agccaagtaa tcacacttcc tgtatatgct tatttaggaa cgaatgtaga aataaatcat
      421 caacctgtaa aggagcaggt gaatgctaat ggattggtac aaataagcgg agtcaaagga
      481 gttgctgata ttaaagtaag cacaagttat acagtaacga ctattatttc ttatcttgtt
      541 acattaactg catatttatg tttatttttt aaaatagttt tccataaaag gtaaattaac
      601 ttttcattta taaaataaat tgattaaaaa ggagaatctg tcgataagga ttctctcttt
      661 tttatacgaa aaatctgtag ggggatgaag taatcgctta tttcctcact ttatgataaa
      721 ttacagctaa agtcactagc ttgtcttctt cggaaaggag gagaatctat ggatgaggaa
      781 caaatattca ttcaaacact ttataatctg attttgaatc cgaatacacg agattgggaa
      841 cgaaaagtat tgattcagac aaagaatgac ataagagaaa atatatccgt taaagaacag
      901 ttatcaaaac tagaagcaac actacgtcca ttagctatcc gaatgaatct gacgcctgat
      961 gtaatggact tttatttatt gattacggaa ggatttgata aggagcaaaa atatgacttt
     1021 tccaagcatg ccatgcaaga tgctgactat caagaacgtg cagtatttgc agggggatgt
     1081 ttctggtgta tggtcgagcc gtttgaatcg aaaaaaggga tcctatctgt tttgtcagga
     1141 tacacgggcg gacatgttga aaaaccaaat tatgatcaag taagtggtgg ttacacaggt
     1201 catgtggaag ctgtggagat catttatgac acaagagaaa taagttatag cgagttgctg
     1261 acaatctatt ggcaaattac tgatcccaca gatacatttg gacaatttca agatagaggg
     1321 aagcaatatc gtccagtgat tttttatcaa gtcgaacgac aaaaagaatt agcggaacaa
     1381 tcaaaacaga aactagacag ttcaggaact ttccaccaac caatcgtcac aaaaatcgaa
     1441 ccagccggta cgttttggcc agcagaaaac tatcatcagc aattttacaa aaaacaaccg
     1501 aaacgttata aaaaaatcca gcaagcacgt aatcaatttc tgatctatca acgagtaaaa
     1561 aacaaatggc aaaaaaatat acgaaaaaat cattttgatt aactcctttt ctttaaaatg
     1621 tagtaaacta aaaatagaaa aaagaaagcg ttttaaaaag atttgatgag gaggagaaaa
     1681 attgtatatt gcagatagtg cacgttatga aaaaatggaa tatcgtaaat ccggtcattc
     1741 aggtttgaaa ttaccgattt tatctctcgg attatggcaa aattttggtg attatgatcc
     1801 tatccataat caaagagaga ttttaagagg cgcattcgac atgggaatta cccatttcga
     1861 ccttgcgaat aattatggag gccctgctgg agctgcagag aaaaactttg ggcgtatttt
     1921 tagagaagat tttcaagcct accgtgatga actgattatt tctagcaaag caggttatca
     1981 tatgtgggaa gggccatatg gggaatgggg ctctcgtaaa tccatcattt caagttgtga
     2041 ccaaagtctc caaagaatgg ggctagatta tgttgacatc ttctatcatc atcgcccaga
     2101 ccctgataca cctttggaag aaacagcaga agctttgatg caacttgtaa gacaaggaaa
     2161 agcattatat attggtatat ccaactataa tggagaagat acaaaaaaaa tgactgaaat
     2221 tttaaaaaga aaggaagcac cgttcattat tcatcagatg cgctacaata tgttttctcg
     2281 tgcactacta gaagacgatc tatctccagt attagaagaa gaagggctag gggcaatcac
     2341 gttcagtccg cttgctcaag ggttgctgac aaatcgttat ctacatggta ttcctgaaga
     2401 ttctagagca catcgtaagg aaataccatt cctttcagaa gaacaagttg gatcaactct
     2461 ggaaaaaatc aaagcactgc aaacaatcgc cgtatcacga ggacaatcat tagcgcaaat
     2521 ggcccttgca tggaatttgc gacaaaaatc tgttacaagt gtactggtcg gagcaagtcg
     2581 gttaagtcag ttacaagaaa gtgttcgaat gatggataat ttagatttct caccagaaga
     2641 agaattgaga atcgaccaaa ttctagaata gaaacgatgt aatcaaactt tttatccata
     2701 tattaaaaaa aacaaaagat gacatcaaga atcgaatgaa gatcgtctgt tattgaacat
     2761 catatttttt gtcgttcaaa ataaaaatga tcgaaggcta ggagtgaatt ttatcctagt
     2821 cttttttatg gtaaatatct tcttcgtttg tatgtctatg aacggattaa tcaattttgt
     2881 atacaaaaaa tcatcagatg attcaaacgt gttataataa ttacgaaaaa atacatcaga
     2941 tgaaaaggag gtatgtggtt tggaagaaaa taaaacccta gtagaagtaa ctactgacaa
     3001 gatccttaga tatttaaagg aaatgcagtt gcaagtagga gataaactgc cgaatgaata
     3061 tgaattgtcc gattatttag aggtggggcg aagcacggtg cgagaagctg tgcgggctct
     3121 agcttccagg aatatccttg aagttcggca aggagcgggg acatttgtca gtgataagcg
     3181 agggattgcc aatgacccac tcggtttttc tttgattgaa gatcaggatc ggatgatacg
     3241 agacttattt gaactgagat atttattgga gcctaagatg gcaggtttgg cagcggaata
     3301 tgcaacgaag aatcaaatcg tccaaatgaa aaaattaacg caaaagattg aaacttcttt
     3361 tcgagaaggc acacaagaac atgttcaatt agacatcgat tttcatacga tgattgcaga
     3421 agctagcgga aacgtagcct tgctgcatat tatcccaatc attaatgaat caattgcttt
     3481 gtttaataaa aattatgata tccagcatct gaaaatcgag acgatcgata ttcatacaga
     3541 gatcgttcag gccatcgaaa ggcggaatgc acttgctgcg tttgatgcaa tgactgtcca
     3601 tatggcaata aatcggagag aattacagaa aataatagga gaaaaagaaa ctaattgaca
     3661 gttcatctga tgtattttat gataaataca tcagatgaat ttttgtttta tatttggtaa
     3721 aaaagaggag taaatagttt ctgttcatcg aaatgtaagc gctgactaat agacaaaaag
     3781 agatgacgtc aaggagaaga agcaggaatg aggggatctg gaatgagttt tatggataaa
     3841 ttagccaatg ttttagggaa cgtggcaacg agaatcaata atttaaggta tatcatggtg
     3901 ataaaaaacg catttgctgc attgattccg gtaattatta cgggtgcttt tggcacattg
     3961 ttttcagcaa tggtctttga ctcggaaaat ggcttagcac agataagttt cttgcgtttt
     4021 ttagaagaat taaagcctat cgcatctgcc atcagctatg tcacactaag tttcttgacc
     4081 atctatgctg tttttctgat agggatcgaa ttagccaagc tgaataaagt ggatggtgta
     4141 ttccctggta tcatcgcagt gatgtcttat ttatctgtta atccaactgt ttatgaatta
     4201 gtgaatgaaa atcagactgt tatagtagaa aacgtattgg ctaaacaata tacagatacg
     4261 aaagggttat ttttgggaat gttggtctcc atcctttcta ttgaacttta ttgctggttg
     4321 aggaaacaga agaaactaca gctgagaatg cctgataccg tgccaacgaa tgtttctgct
     4381 agtttttctg ctttattccc gacgattctg actgtatcgg gtatcgcggc tgcgggattt
     4441 atcatcaaag aacttacagg aatgtacctt tatgatatca tttacaatgt cgtacaacgc
     4501 ccattagaag gtgtcgtaca agggttaccg ggtattttgc tattgatgtt tattgcgcaa
     4561 gtcttttggg tgattggtat ccatggaaat caaatggtga agccgatacg ggaaccgttg
     4621 cttttagcgg caattgctgt aaatacggaa gcttttgaag ctggaaaaga agtaccgaac
     4681 attattacta tgccattttg ggatatgtat atgagtatgg gcgggtctgg agtcacgatc
     4741 ggtttactga ttgcagtatt gttagttgga aaacgggaag atatgaaaca aatcaccaag
     4801 ttatcccttg ctcctgggat cttcaacatc aatgaaccag ttatttttgg tatgccgatc
     4861 atgctgaatc caattttagc tattccgttt atcttaacgc ctcttatcac tggaacgatt
     4921 ggttattttg caaccgcttt aggatttgcg gcgaaagcag ttgtaatggt cccttggcca
     4981 atgccgccga tcgtcaatgc ttatctggca acagcaggaa atatcggtgc agttattacg
     5041 caacttgttt gtattattgt ttctattttg atctatctgc catttgtaaa agtagcaaat
     5101 cgtgtggaaa atgatgggat ctaaaaaaca ggaggaaatc atgaaaatta ctattccaaa
     5161 aaattttatt cttggtgctg catcttctgc ttggcaaacg gaaggttgga aaggaaaaaa
     5221 agaaggacag gattcgttta tagatagctg gtataaaaat gaacattttg tttggcataa
     5281 tgggtatggc ccagccgtag caactaattt tatggaacaa taccagcagg atgtggatct
     5341 gatgaaatcc atccatctga cacattatcg gacttctatc aattgggctc gtttttttac
     5401 tgattatgag aactgtatag tagatgaaga atatgctcag catataagtg aaatgatcga
     5461 ctctttgatc gaagcagacg tagaaccgat gatttgtttg gaacattatg aagtcccagc
     5521 ttttcttttg gaaaaatatg atggctggtc ttcaagaaaa gtagttgatt tttatgctaa
     5581 gtatgctcaa attgcctttg atcgttacgg tgatagggta aaaaactggt ttacattcaa
     5641 cgaaccaatc gttccccaaa cgcgttgcta tttggatgct gttcgctggc cgcatgaaca
     5701 aaacacaaaa aagtggatga tgtggaatta tcataaagtt ttagcaacgg ctaaagcagt
     5761 agagatttac cgcagcggaa attatggtgg aaggattggc tgtattttaa atcctgaaat
     5821 ggtctatgct cgatccaatt ctccagaaga ccaaaaagct gcttatatgt atgatttgtt
     5881 ttataatcga gtattttttg atccgatggt aaaaggatac tacacagaag agttcctgga
     5941 gttgtgtcaa aaacaatcga tttatttcga tcctgcaaca gaggatttag aaacgattca
     6001 gcatcataca gtagatttcc ttggaatcaa tcaatattat cctaaacgcg ttcgcgcccc
     6061 tcgttatcaa tggaatgaag agacaccttt ccatcctgaa cgatattatg aagtctttga
     6121 tttacctggg aagaaaatga ataattccag gggatgggaa atctatccga aaatcatgta
     6181 tgatatagcc atgtatctaa aagaaaatta tcataatatc ccttggctga taacagaaaa
     6241 cggtatggga agagaatatg aagaacagta tatggatgag aaaggaatcg tgcaagatga
     6301 ttaccgtatc gaatttatcg gacagcattt gcattacttg atcaaagcaa cagaagaagg
     6361 cgcaaattgc gaaggctata tgttatgggc attcactgac tgtgtgtcac caatgaatgc
     6421 atttaagaat cgatatggat tagttcgaat cgaattgaac gatgaacgaa gcagaacatt
     6481 gaaaaaatct gcttcgtggt atcagacttt gattgataag agagaattgg atttgcctga
     6541 cgaaatctat aaataaatag aagttttttg tagatgttca ttgtatttac tggttatttg
     6601 aaataaaaga ggttgtgaat ccagcgtttt gagctaatac ccagggtcag ctgattgaac
     6661 accgtttatt gagaggttgt gaaaaagctg tcctgcatca agtaataaga atagcaaagt
     6721 aaaaacaaca tcttatgttt ttaacttggt tggacttatt accgcagatg caagcttttg
     6781 aataccgttt atttgaaaaa agttgtgaca agttttgtgt cacaactttt tttgtgcacg
     6841 ttaaaagaaa aaaggtaata aaaattaatt tgtgtgttta gtgtgcgctt tcttttttag
     6901 tgtgtatgca acttggtata gaccttaaat gtgatgatat caacttttta aagcgctttt
     6961 aaagttggca cgctacttgc ttatatacaa gtgtaagtat agaaaggaac gtgtttaaaa
     7021 tggaaaagaa aacaatcatg cttgtatgtt cagctggaat gagtacaagt ctcctagtca
     7081 caaaaatgca aaaggcagca gaagcaaaag gaatggaagc agacatcttt gctgtatctg
     7141 cttcagatgc ggataatcaa ctagaatcta aaaatgtaga cgttttactt ttaggaccgc
     7201 aagtacgctt tatgcaaaat caatttgctg aaaagctagc acctaaaggc attcctttag
     7261 atgtaatcaa tatggctgac tatggcatga tgaatggtga aaaagtactc gaccaagcag
     7321 aaaaactaat aaacaaataa taaaaattat cggaggatac aataaatgga agatcaaaaa
     7381 aatctagaag ccatcatggg tctgatcatg tttggcggaa atgcaaaaag cgatgcaatg
     7441 gaagcgattg ctgctgcgaa aaaaggtgat ttcactttag cagatgaaaa aatcaaagat
     7501 gcagaggaat ctttagtcca agcacatcac tctcaaacag agatgctgac acaagaagca
     7561 caaggaaatc acgttgaagt cacgcttttg acggtccaca gtcaagatca tctgatgaca
     7621 tctatagctt ttacagattt agcaaaagag atcattaatc tgtatcgtcg attgaacgcg
     7681 taatctacag taaagaaaag agggaagatt tatggataaa tttgttgcat ttatggagaa
     7741 acattttata cctgtagcat caaaaattgg tgcacaacgg catttagttg cgattcggga
     7801 ctcatttatg gtgagtatgc cgttaatgat cttaggagca ttagcagtaa tgatcaataa
     7861 cttaccgatt cccgggttcc aagaattgat gaactcaatt tttggtggtg aatcatggaa
     7921 aggattcggg gctgctgctt ggaacggtac atttgccatt ctttctgtct tgattgcttt
     7981 cttgcttgct tatcatttag ctaacggcta ccgaaaagac ggtgtttccg caggtgtgat
     8041 ttctttaggc tctttcttcg ctttaggcgg agcattaggt atgagctcaa acggtttgtt
     8101 tattgctatt attgttggga ttatttcaac agaaatcttt gtacgtttaa gcggaaatga
     8161 aaaattgatc atcaaaatgc ctgatggtgt gccaccggca gttgcaaaag catttgcttc
     8221 attactacca gcaatgatca ctatctctgc gtttgcttta gttgctgcaa tttttgctgc
     8281 attcggtgtt gatgatatcg taggttcttt ctatacagtg gtccaagaac catttatggg
     8341 acttgcaaac tcttatccat ctgccttact gcttgcattt atcacaccat tcttatggtt
     8401 ctttggttta cacggagcga atatggtcga tcctttgatg caaacaatca atgcgccagc
     8461 cattgaagca aacgttaatg caatcgcagc tggacatagc gcaccgttta tcgtgaacaa
     8521 acctttcttt gacagctttg tcaacttagg aggaacaggt gcaactttag gtttactgtt
     8581 agctatttat ttagtaggac gtaaaaacaa accatatatg gttgtaacaa acttgtcgat
     8641 tgcgccaggt gtcttcaata ttaatgaacc aacgatgttt gggttaccta tcgtgttgaa
     8701 cccgatcatg ttcattccat ttattttgac accaatggtc ttagttagtg ttgcttactt
     8761 tgcgacttct acaggtttag tgccagcagc taccgttatg cctccatggg taactccacc
     8821 aattattggc ggtgtattgg caacaaaaag tattgcaggt ggtgtactag ccgctgtcaa
     8881 tttggttctt tctattttga tctatctacc gttcgtcaag gtagctacga tccaagaaag
     8941 aagaaaagaa gctttagatg cttgattaat caaaaaacgg agaacaagtg attgttttcc
     9001 g
//
LOCUS       DACDMM010000106         8919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDMM010000106 DACDMM010000000
VERSION     DACDMM010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.107"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="IWD16_002001"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="IWD16_002001"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9089010.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1711..3057)
                     /gene="gor"
                     /locus_tag="IWD16_002002"
     CDS             complement(1711..3057)
                     /gene="gor"
                     /locus_tag="IWD16_002002"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP9089011.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3191..4432)
                     /locus_tag="IWD16_002003"
     CDS             complement(3191..4432)
                     /locus_tag="IWD16_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9089012.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(4450..5373)
                     /locus_tag="IWD16_002004"
     CDS             complement(4450..5373)
                     /locus_tag="IWD16_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9089013.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5594..8086)
                     /locus_tag="IWD16_002005"
     CDS             complement(5594..8086)
                     /locus_tag="IWD16_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9089014.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8093..8560)
                     /locus_tag="IWD16_002006"
     CDS             complement(8093..8560)
                     /locus_tag="IWD16_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP9089015.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8696..8769)
                     /locus_tag="IWD16_002007"
     tRNA            complement(8696..8769)
                     /locus_tag="IWD16_002007"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8733..8735),aa:Arg,seq:acg)
     gene            complement(8827..8900)
                     /locus_tag="IWD16_002008"
     tRNA            complement(8827..8900)
                     /locus_tag="IWD16_002008"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8864..8866),aa:Pro,seq:tgg)
BASE COUNT         2478 a         1884 c         1485 g         3072 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
     1081 aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
     1141 ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa
     1201 aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
     1261 ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca
     1321 cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg
     1381 tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt
     1441 aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac
     1501 ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat
     1561 ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact
     1621 aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa
     1681 taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc
     1741 tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc
     1801 cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat
     1861 tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa
     1921 tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt
     1981 ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac
     2041 tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata aacgttctga
     2101 taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc
     2161 aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt
     2221 tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc
     2281 accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata
     2341 ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc
     2401 ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg
     2461 ttcttttcga tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc
     2521 agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa
     2581 aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc
     2641 tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg
     2701 actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct
     2761 ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa
     2821 atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc
     2881 caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt
     2941 tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt
     3001 tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt
     3061 gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa
     3121 aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc
     3181 agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg
     3241 gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat
     3301 gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat
     3361 ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag
     3421 ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt
     3481 cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc
     3541 ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt
     3601 ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc
     3661 cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg
     3721 acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt
     3781 ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac
     3841 atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat
     3901 aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa
     3961 tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt
     4021 ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg
     4081 gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg
     4141 cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt
     4201 catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa
     4261 ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat
     4321 tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg
     4381 ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca
     4441 tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg
     4501 gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca
     4561 gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt
     4621 ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc
     4681 attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc
     4741 ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc
     4801 acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt
     4861 ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc
     4921 ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc
     4981 ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat
     5041 atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac
     5101 tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat
     5161 tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc
     5221 taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa
     5281 ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc
     5341 aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat
     5401 agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa
     5461 ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt
     5521 gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac
     5581 acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg
     5641 tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa
     5701 gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg
     5761 caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt
     5821 tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga
     5881 ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca
     5941 agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt
     6001 gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg
     6061 ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag
     6121 aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg
     6181 ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca
     6241 actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac
     6301 tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca
     6361 tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca
     6421 tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac
     6481 gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca
     6541 gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact
     6601 ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt
     6661 ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta
     6721 attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt
     6781 catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga
     6841 ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga
     6901 tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat
     6961 ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg
     7021 gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt
     7081 tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg
     7141 gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat
     7201 gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg atgacctttt
     7261 tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa
     7321 gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag
     7381 ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt acagggttat
     7441 tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa
     7501 ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg
     7561 taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca
     7621 ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt
     7681 ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta
     7741 ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc
     7801 tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag
     7861 ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt
     7921 cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg
     7981 ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg
     8041 caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc
     8101 catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt
     8161 catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt
     8221 tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac
     8281 tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc
     8341 ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact agataaccac
     8401 gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga
     8461 ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta
     8521 tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact
     8581 aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta
     8641 aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattccgt atcgacgcgc
     8701 ccaagaggag tcgaacccct aaccttttga tccgtagtca aacactctat ccagttgagc
     8761 tatgggcgca tattcaattt aaaaaaatta taaatcattt gaaaacaaaa agcaagataa
     8821 aaaaaatcgg gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta
     8881 ccaagctgag ctacttcccg tttaaaaaat aaaaaagac
//
LOCUS       DACDMM010000107         8905 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDMM010000107 DACDMM010000000
VERSION     DACDMM010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.108"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(409..906)
                     /locus_tag="IWD16_002009"
     CDS             complement(409..906)
                     /locus_tag="IWD16_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9089016.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(946..1755)
                     /locus_tag="IWD16_002010"
     CDS             complement(946..1755)
                     /locus_tag="IWD16_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9089017.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2089..2946
                     /locus_tag="IWD16_002011"
     CDS             2089..2946
                     /locus_tag="IWD16_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9089018.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2939..3841
                     /locus_tag="IWD16_002012"
     CDS             2939..3841
                     /locus_tag="IWD16_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9089019.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3874..4224
                     /locus_tag="IWD16_002013"
     CDS             3874..4224
                     /locus_tag="IWD16_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9089020.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4258..5195
                     /locus_tag="IWD16_002014"
                     /pseudo
     CDS             4258..5195
                     /locus_tag="IWD16_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            5273..6166
                     /locus_tag="IWD16_002015"
     CDS             5273..6166
                     /locus_tag="IWD16_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9089021.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6204..6767
                     /gene="amaP"
                     /locus_tag="IWD16_002016"
     CDS             6204..6767
                     /gene="amaP"
                     /locus_tag="IWD16_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9089022.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6781..6969
                     /locus_tag="IWD16_002017"
     CDS             6781..6969
                     /locus_tag="IWD16_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9089023.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            6982..7884
                     /locus_tag="IWD16_002018"
     CDS             6982..7884
                     /locus_tag="IWD16_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9089024.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7916..8158
                     /locus_tag="IWD16_002019"
     CDS             7916..8158
                     /locus_tag="IWD16_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9089025.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8414..8785)
                     /locus_tag="IWD16_002020"
     CDS             complement(8414..8785)
                     /locus_tag="IWD16_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089026.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3101 a         1539 c         1726 g         2539 t
ORIGIN      
        1 taggttcgag tcctacaagg tacataagag gttgtgaaaa agctgccctg catcaagtaa
       61 taagaacagc caagtaaaaa cagcttctta tgtttttact tggctgttct tattactgca
      121 gatgcaagct tttgaatacc gtttaaggaa gaggttgtga aatcagcgtt ttgacctgag
      181 tattagctta acaaacggaa aaatagttcc tcatattttg ccgtttgtta agctaatacc
      241 gagggtcagc tgattgaaca ccatttaaga aagaggtcgt gagcctgccg tttcactctg
      301 tgtcataaaa aagccaatct actttgatag aaaaagcacg taggactaaa acagttctgc
      361 gtgctttttt tattgtgtgt taaatatcgt aaatgaagat agatattttt atgattcgac
      421 gatagagacg ttgaaatctt tcaacagctc taaagcggat tctccgtgtt ttctactgga
      481 gactaacatt ggatcaacaa aaaagtctgc ttcaggaaaa gcactctgca ggcctatcag
      541 attagagagt acacacaagt ttgtttcaat cccgactact tcgatctctt tttgttcttc
      601 tttgctttca aataaagttt cctggatttc agctaaagtt tcaggtggaa tcgtaaagta
      661 atacttttta atcactcgtt cattttcttg tggagaaaga agagggatca attgcaaatc
      721 ttctcgttct tcttctttgt ccttcagttc aactggtata tcccgtgtga agagaatata
      781 ttcattattt tggcgtgctt tagctaatct ttgagcaata cgttctgcca attgatctga
      841 ctctggaata taaaattcgc tgtttggatc aagaatatga ttttgcatat caataataac
      901 taacattttc tttcctccta cattcatatc gtcattttat tctttttaat agaagttttt
      961 taaaatataa gcaacaccag cttcttcatt cgtcagtgtt acttggtttg caatttcttt
     1021 tacttgtcgc tgtgcattcc ccatcgcaat actcaacccg ctgtaattca acatagcaat
     1081 gtcgttttct tgatcgccaa aagccatgat gtcactcgat tcgattccaa ggtaatccgc
     1141 cagaaaaaca agtgaatgag ctttagtgat acctgtaggc gtgatttcta agaaatgcgg
     1201 atcagagaat actgattgga taccatctgc ttgcagatct ctttgtagct tctgtagttt
     1261 ttcttcactc ttatctgtta atgctaattt aagatagcta agagaaggat cttccaacat
     1321 atttaaaatt tgatcaaaag aatggttctg taaattgaaa tgttcataat aagggtcatc
     1381 tttgactaga tttgattcat cgaatgtcag ctgatcatct tgggtttctc ggctagcaat
     1441 gatggatatt ttcgcttctt gagcatagaa gaaggctttt ctcagtgtat cgctatctat
     1501 ttttgaacgt ctgagaactt tcgccatttt tccatccttc atttcagcaa tcagtgaacc
     1561 attggaagaa atcaaatagc caccgattcc taattcggaa aaaatatctt tagcagaaat
     1621 gctgttcctt ccggtagcga cggttacgat tccgccattt tcatcaaatt tccgaagtgc
     1681 ttggatattt tcttctggta tctttccatt tcgatcaagc aaggtaccgt ctaaatcaat
     1741 cgctgctagt ttcattttat cttcctactt tctttttaca tatctatttt cctgttacct
     1801 taagattaaa tgagaattta aaaaaatgaa actaacttgc tttgtcatta tgaatttttt
     1861 caaaaaacat tcttaagaaa aaggtggcta tatatagtag tttttggtca atacctttga
     1921 ataaaaagcc atgaaaaacg aatagttagc gcttaccgat gcgttttgtg ataaaaaaat
     1981 tttaaaatat tcaatgaaaa caaagcatat attttgcttt aaaaccaaat gaggaatatt
     2041 ctagatgtag aacaggttag aaaaagatta gaaaaaggag tgattttgat ggagaagaga
     2101 tacggcgtag ttcctgtggt aactgcttca gacgaaaatt atgctcctta cttgagcgtg
     2161 atgatcgcaa ctgcattaga aaattgcaat aaaacaagac gcataaaatt ttatgtgatt
     2221 gacgatggtc tatcagaata tagtaaagaa ggattggaag aaacggtaaa taaatattca
     2281 agtaatgcaa gtatccaatt cctgacagtg gaaaaagata tctacgaaga cttcctagtc
     2341 agtgaccata tcacaactac cgcttactta agaatttctt taccaaactt attagctaaa
     2401 gaggattata agaaagtcct ttacttagat tcagatgtgt tagtactaga cgatatcgta
     2461 aaactatatg atgagccgtt aaatggaaaa acaatcggcg ctattatcga tccaggccaa
     2521 gtgaaagctt tggaaagatt aggaatcgat tccgatgact tgtacttcaa ttcaggagtg
     2581 atggtgattg acattgatca atggaataaa aaagaaatta cagaaaaaac tatccattac
     2641 ctcagtgaaa atggcgatcg aatcatctat cacgaccaag atgctttgaa tgctgtactt
     2701 tatgaagatt gggaacaact tcatccaaaa tggaacatgc agacttcgct gatttttgaa
     2761 cgtcatccag caccaaatga aaaatatgaa cgtcagtata aagaaggaaa tgaaaaacct
     2821 tcgatcgttc actttacagg acatgataaa ccttggaata cgttgaaaga tcatccgtat
     2881 accaatttat acttaaaaaa attagcacac agtactctaa cgaaagtagg cgagatcaat
     2941 gaataaaaaa gaaatagcag tagtcgcaag ttgtaacaca aaattcgtac cacatttagc
     3001 tgcattattt gtctcagtat tagataattg caacccgtct aaatttgttc ggttctatgt
     3061 gattgacgat gatatcgatt ttgaaagtaa acagctttta cgcttctcag tgaaaaacgc
     3121 acgaatgaac tcagatgtgg aattcttgaa aatcaataaa gaattcttta caaatgtggt
     3181 aatcagcgat cgtattccag aaacagctta ttatcgtatt gcgattccgg aattgttccg
     3241 cggcacagaa gttgaacgta tcttatatat ggattgcgac atgatcgctt tacaagatat
     3301 ctctaaatta tggcgtcttg acttcggtga ttcaatcgta gcagctgttg aagacgctgg
     3361 gttccatcaa cgactggaaa aaatgaaaat ttcagctaaa tcaatgcgtt atttcaactc
     3421 aggtttgatg ctgatcaatg tgaaaaaatg gttagatgaa aatattacgc aaaaagtatt
     3481 agatttcatc gaacataatc cagaaaaatt acgattccat gaccaagatg cattaaatgc
     3541 tatcttacat gaccgttggt taccacttca tccgcgttgg aatgcacaag gctatatcat
     3601 ggcaaaagca aaaaaacatc caacagcagc aggtgaacgt gaatacgaag aaactcgcaa
     3661 caatccttac attatccatt tttcaggaca tgtgaaaccg tggagcaagg attttgaagg
     3721 gccaacgaag aaatattacg aaaaatatgc agggatgacg gctttccgtt gtgtagctaa
     3781 attccctaaa tatccaaaat atgcaaaagt tcaaccaaaa acagatgtag ttgttaaata
     3841 atagaagaca acaactgaag agggggagta ggaatggaaa ttacaatgtc taaaaatgca
     3901 gtagagacgt taattgaaaa ggttggggaa aatacaaaaa tcgcactcgc gcttatcaat
     3961 gattccgatc catttctaag ggacaaaggt gcttttgcaa aaggcagctt ttttcaaatc
     4021 attccttttg tttcggaatt tggagaatat gcgactaaaa tcgagcatcc attattggac
     4081 atctatacat ctaaattaga gcaaaattat ttcggtaaac gattgaacat ggacttcaac
     4141 aaacagcttg acagcttttc attagaaaat gaaatcgcag tactcgatta taatatcaaa
     4201 ttaaagaact gcttcttcag ctaatagttt cgagaaagtc caaaaaggag gtatacattg
     4261 agatactact ttgtggatga agaaaagaat gtaatagaaa cagatgaaaa aaaatataat
     4321 tggctcgtac ttgatagtgc aaagcccgaa gagattgagc aagcaatcga agaattcgag
     4381 cttcctgatg atattttcgt aggaaccgaa tatccggaag aagtttctcg gatggagcgc
     4441 ctttatgaga cgaaactaga gcatccattt tctttggtag tgattaatct agatcagtca
     4501 gataatcgta tcgaaaaaaa ttaacgccaa tatcttttgt actttccgat aatctcttga
     4561 ttacttgtat cgatagaaaa accgaatttg tggatcggct gctgacaaag catggaaatc
     4621 atttaaactc gtttgaaaag atcatcacgt atacattatt agatatttac acccattacg
     4681 tgaaagaact aagagagatg aaaaagcgaa ttgacgcttt ggataaagca gctcgtaaaa
     4741 caacggaaaa tgaagaactg tataaacagg cagatcttga aagagacatc gtatatatcg
     4801 atcatacctt aagagatcaa cgggaaacaa tggatcaatt atggaattct gaaactttca
     4861 tcaaacgttt agacgatgaa cagctactct atgatgtcca tttgagacag cgacaaacag
     4921 aaaaaatgat cgagatctat cgagatttat tagagagtat tggtgatcta ttcaacggga
     4981 tgatggacaa taatctaaat catatcatga aatatctaga ttcagctgca ttaatcattt
     5041 ccgttccggc gttgttcgca gggatttggg gaatgaatac aggaggatta ccgggtaaaa
     5101 gttctgctct aggctttata ctcgtagtct ttggctcttt gatagcagca gtagttttag
     5161 gttaccattt ataccggaaa gattatacaa attaaagaat ctatttaaaa caggtatcaa
     5221 acaaagaaat ttgttttggc aatacaattt aaaaactagg aggaatacag caatgacaga
     5281 accaaaatta atcgacccgc gaaacttgta ccatacggag aaatttccta aacaagatca
     5341 ggatacacca gctttacagc agaatatgat tcctgttcct gattgtggag aagaatccta
     5401 tgaaggcaat aatcaactag aaaaccgccg tgtactaatc actggtggag attcagggat
     5461 cggtcgtgca gcagcgattg catttgctag agaaggggca gatatcgctc ttcaattctt
     5521 cccaggagaa gaaaaagatg cagaggaagt agcaaaatac gtgaaagttg caggacgtaa
     5581 gattgtctta ctaccttttg atcttcgtga cgaacaagca cctaaagaaa tcatagataa
     5641 agccgtgagt gaacttggcg ggctagatac gatggtacta aacgcaggac agcaaatttc
     5701 ttgtcagtct attgctgagt taccacttga gcaagtcaaa gacacattta tgataaacat
     5761 catcgctatg tttgctttag tgaaagaagc tgttccgcat atcccagcag gaggagcaat
     5821 cgtaacaaca acttctgttc aagcattcaa tccaagcgaa catttattag attacgcagc
     5881 aacaaaagct tcaatcgcta actttacgat cggtttagca aaacagcttg cacctaaagg
     5941 aatccgtgta aatggtgtag cacctggtcc aatctggaca ccattacaac tagacgaagg
     6001 acaaccttct gattcgatcc ctgaatttgg tcagcaaagt ttactagaac gtgcaggtca
     6061 gccaggagaa ttagcaccag tttatgtttt cttagcatcg aataaagcaa gttatgttac
     6121 agcccaaatt tacggagtaa ctggtggaga agcgatcaac ttataagaag ttgatcgttt
     6181 cacccctatg aggaggtgcg aaaatgaata aaggtgtaaa aacgatcgga gtgatcggaa
     6241 cattactttt gctatctgtg cttttgtttg cgatactatt aagtgcaacc tatccattgc
     6301 cttttcagct agagaagttt cgattctttt ccatcactaa ttcatacgtg caacaatatg
     6361 tattttggct tgccgttgca tttgctattt tgacaattat cacgttactt gttttcctgt
     6421 tttatccaaa aacaatcgga aactttctac tgaaacatgg tgatggggag ttgacgttgg
     6481 ataaaaaagc aattgaagga atggttcgtt ctcacttgca tgaagatgaa ttcatccatt
     6541 caccaaaagt aaatgtcaaa gcaacgaaaa acaagatcgc cgtcaatgta aaaggggagt
     6601 tgaaacgaac ttcagcactc attggtaaaa caggaacatt gatgaaagaa atcgaaaatg
     6661 aagtacaaca catattggga acaaaagagc cagtgaaagt atcggtgaaa tacacaggct
     6721 ttgaagaacc agctaaacga agcaatgacc gcaaacgagt agaatagggg gggaccaaaa
     6781 atgaaagaac tattttcgac atacaagctc cctatcatct ttggggtact tggattgata
     6841 ttagccgttt tatggatcac cgtgggcttt ttcaagacct tattgatctt gatcatggca
     6901 gcgatcggcg tcgctatcgg tttttatctg aaacaaacga aaatcttaga tcactatttt
     6961 aaatcttagg aggaaacatc catgaataac aataacaaaa ataataacaa taaaatgaac
     7021 aaccaaaaac cagtgtctgc acatccagac ccaacgacac caaaccaatc aaatggtgtg
     7081 aacgatgaaa agaacaaaac agcaccagta tctgcacatc cagatccagt agtccctaac
     7141 gttcaacaag gagtagaaga gaagaaacat gatgcagaaa agcatgcacc tcatcatcca
     7201 ggaactccag aatctaaagt tccagaacaa catgatgcaa tcgatccttc taaacctgtg
     7261 gaagagcaaa aacatccttc tgaccatgcg aaagcacaag aaaaacttag ttctgaccac
     7321 acaaacggac aaggaaaact tggttctgat catacaaata accaaggaaa gcatgattct
     7381 gaacaggaag attcaatgcg cggtgagctg aattatgaag ataaagttgt gcaaaaaatc
     7441 atcggaatcg caattgaaca agtagacggt ttattatctg caaacggtgg tttcttctct
     7501 aatgtggcag gaaaacttgt gaatacagat aatgtaacgg ctgggatcga aacagaagtc
     7561 ggtaaaaaac aagtagcagt tgatatggat gtcattgtgg aatatggcaa agatatcgaa
     7621 aaaatctttg aagaaatgca agaagtaatc ggcaaagaag taaaaaatat gacacattta
     7681 gaagtgatcg aagtcaacgc taacgtagtg gacatcaaaa caaaagaaga atttgaaaaa
     7741 gatcaagaaa cggtgcaaga caaagtaact gacgcagcga aaaaaaccgg tgaatttgct
     7801 tcgaatcaaa cagataaagt aaaatcagca gctggaagcg gcgcacaaaa agtaaaagaa
     7861 aatacagaac cacgggttca gtaataagga agtaaaacat taggaggaac gaaacatggg
     7921 atttatttgg tcacttatcg tcggaggaat tatcggagca attgcaggag caatcacgaa
     7981 taaaggttct tcgatgggga ttattgcaaa tgtcgtagca ggtttagttg gatcagcact
     8041 tggtcaagca ttattaggtt cttggggacc aagtattgca ggaatggcaa tcgtgccatc
     8101 attgattggt gccatcattt tagtactaat cgtatcattc tttgctcgaa aactgtaaaa
     8161 agacttttaa aaagaggtcg tgaaaaagct gttcttcatc aagtaataag aacagccaaa
     8221 caaaaatagc ttctcatatt tttcatttgg ttgggcatat tatcgaagat gcaagctttt
     8281 gaacactgtt tatacggaat aagaggctgt gacaagactt ttgtcacagc ctcttattta
     8341 tgcgggcaaa ttcccaatta gatttttaaa agtttttcct taaatgtgtc tttattgata
     8401 cagagaagct tctttaataa aacaatgcct ccctaaattt tcttgagata ggaaaggtga
     8461 acatacaaaa taatacgacc actttgaaaa cccagatgat ccacaaatgg atcgtatgat
     8521 actgatgaag taaaaagcaa tggatcagaa actcaaagag attgaaaaag acaaggaaga
     8581 aataatatct cttttttact cttgtctgtt ttagccaata atagctcgta aagtaatcta
     8641 gacaaacaaa tagaagtaaa caaagtttcg taagagcagc aatcgtatca tgttgctctg
     8701 ctagacgatt gacgagcagg aaaaatgttg gtaatggaat atagatcacg agtaatgaaa
     8761 gaaaaaagta aacaataaat ggcatactat tcctcctaat tgtgtgagtt cgccctcatt
     8821 ttaaaataac gatatttgtt tgtgaagctt tttcgtgtcg taacaaatgt tatctaatgt
     8881 atttttaaat aaataatcat tatta
//
LOCUS       DACDMM010000108         8890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDMM010000108 DACDMM010000000
VERSION     DACDMM010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.109"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..2181
                     /locus_tag="IWD16_002021"
     CDS             1..2181
                     /locus_tag="IWD16_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9089027.1"
                     /translation="MIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSGG
                     YRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDFR
                     YDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVIIR
                     STQLVNNSDKTVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLDS
                     KRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGINS
                     FNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLINN
                     WEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLACG
                     LENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDFS
                     RQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLYE
                     FLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVYP
                     ISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAFY
                     KKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLAG
                     LAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            2242..3462
                     /locus_tag="IWD16_002022"
     CDS             2242..3462
                     /locus_tag="IWD16_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP9089028.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            3459..4919
                     /gene="gtfA"
                     /locus_tag="IWD16_002023"
     CDS             3459..4919
                     /gene="gtfA"
                     /locus_tag="IWD16_002023"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP9089029.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            4996..6102
                     /locus_tag="IWD16_002024"
                     /pseudo
     CDS             4996..6102
                     /locus_tag="IWD16_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            6424..6633
                     /locus_tag="IWD16_002025"
     CDS             6424..6633
                     /locus_tag="IWD16_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9089030.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            6626..6775
                     /locus_tag="IWD16_002026"
     CDS             6626..6775
                     /locus_tag="IWD16_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP9089031.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            6810..7594
                     /locus_tag="IWD16_002027"
                     /pseudo
     CDS             6810..7594
                     /locus_tag="IWD16_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(7878..>8890)
                     /gene="xerS"
                     /locus_tag="IWD16_002028"
     CDS             complement(7878..>8890)
                     /gene="xerS"
                     /locus_tag="IWD16_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP9089032.1"
                     /translation="QDREDKDQSPRTTLEYLKNYKLFYEWLLSESIVPDTSITSIRQL
                     TAYDLNLYKSHLKRRTKENVKKETTKTKLKDNNNLGLSTSTINRNITALKVLFKYLSK
                     SSNNPLGKPYLDNNPMDQVATITDKTTLAARANAIEKKLFLDKDTQNYLDYIANEYKN
                     TLSKRALIYYQRDVERDLAINALILGSGLRLSEVININLDDLSLDKNNVVVTRKGNKR
                     DAVNIAAFAMEYLANYLAIRKERYKVTENEKALFLAIYQGEAKRISGIAIERMVAKYS
                     KGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQLGHSSTNTTTLYTHIDNAATIDALN
                     AL"
BASE COUNT         3060 a         1371 c         1699 g         2760 t
ORIGIN      
        1 attatatttg acaatcacac acaaacattt catttacaaa ataaaaaaat tagctatcta
       61 ttaggaatcg aagaaaataa ttatttaacg catctttatt ttggaaaaaa gattacgcac
      121 tattctggtg ggtatcgcta cccccgaaca gatcgttcct tctcacccaa caatgcaaat
      181 gcgcaagatc gtttattatc tttagacaca ttaccattag aatttccagg atatggacat
      241 ggtgatttta gagaaccggc gcataatatt aaattaggaa acggtagtcg tgtaaatgat
      301 tttcgttatg atagctacga aattcaaaaa ggaaagccca gcttagcggg attgccagcc
      361 ttatatacgg aagatgatga tgaagctgaa acgctgatta ttcaattgaa agacgcgatt
      421 agtggattga ctttacagtt gttctacaca atttatcaaa atcacagtgt aattattcgt
      481 tctacgcaat tagtaaataa tagtgataaa acagtggaga ttaaccgctt agctagtcaa
      541 tcaattgatt ttccaaatcg tccactcgag ttaattcatt taaatggtac ctgggcaaga
      601 gaacgtcaaa tgactagaga aaaaattcat acagggataa aagtattaga tagtaaacgc
      661 ggatcaagca gccatcaaca aaatccgttt gttgctttag tagatgccaa aacagatgaa
      721 tttcaaggag aggcatatgg atttaattta gtttatagcg gtaatcacga aacggtgatt
      781 caaaaagatc catttgacca aacacgggtc attatgggaa ttaattcatt taattttggt
      841 tggcaactaa aaactggcga ctcatttcaa acgccagaag tcgtgatggt ttatagtaat
      901 gaaggtctga atacgttatc acagacgtat catgaattgt ataatcaccg tttaattcgt
      961 ggaaactatc gtttaaaaga acgtcccacg ttaatcaaca actgggaagc aacgtatttt
     1021 gattttaacg aagaaaaaat catgaatatt gtcgatgatg cgaagaactt gggcattgaa
     1081 atgtttgttt tagatgatgg ttggtttggc ggtcgtgaaa atgacttcac ttcattaggt
     1141 gattgggttg aaacagaagg caaacttgct tgtggtttgg aaaatttagc caagcaagtg
     1201 catgataacg ggatgaaatt tgggatttgg tttgaaccgg aaatgatttc tgaagatagt
     1261 gaactatttc gagcacatcc agattgggca ttgggtattc caggtcgtga ccgctcaatt
     1321 agtcgaaatc aatatgttct tgatttttca agacaagatg ttcgtgataa tatctatcaa
     1381 caaatgacgg cgatttttga tcgtgtaccg attgattatg tgaagtggga tatgaatcga
     1441 aatatgacag aagtctattc tgtcttgttg gactcaacga tgcaaggtga agtggctcat
     1501 cgttatatgt tagggttata tgaattttta gaaaagttaa caacgaatta tccaactatt
     1561 ttatttgaaa gttgttcggg tggaggcggt cgttttgatg caggtttctt gtattacatg
     1621 ccacaaactt ggacgagcga caataccgat gccgtggctc gtctgaaaat tcaatatgga
     1681 accagcttag tgtatccaat ttcaagtatg ggtgcgcatg tttcagcagt cccaaatcat
     1741 caaacaggaa gagaaacgag tttaactatt cgtggcaatg ctgcgatggc tggtgttttt
     1801 ggttatgagc tagatctaac gcaattaact tctgaagaaa aagcagaaat taaagaacaa
     1861 gttgcctttt ataaaaagca tcgccaactt ttgcaatatg gaacttttta tcgcttggaa
     1921 agtccgtttg aaggcaatga tactgcatgg atgtttgtct caaaagacca aacagaagcc
     1981 atggtctttt atttccgagt attagcagaa gctgcgcatc cattagtcac attgaaatta
     2041 gcaggattag cagaagaagc aacttatcaa atggaagatg taacatttgc tggcgatgaa
     2101 ttgatgaatc tcggttttta tattgactca gaactacatg gtgattatgc gacacaacgc
     2161 ttttatttga aacagatata gtagcttgaa tttacaatat aagtgcaaca ccaaaagaga
     2221 ctcattccaa acgaagaaaa tatgttttat ttacacgcat ttactaaaaa gcagcctgat
     2281 ttgaattggg aaaatgaaga agtacgtgaa gaaatctatg caatgattaa ctattggtta
     2341 gataaaggac taggcggatt tagaatcgat gcgatattaa acatcaaaaa aaaactagag
     2401 tatggcatct ttgaatctga tggagaagat gggttagcct tcattgggcc ctggattctt
     2461 aatcagtcgg gcattgaagt gtggttaaaa gaaatgagag agcggacatt taaaccacat
     2521 aatagtatga cggtagcaga agccgatgtt cctaatgaac gtttagatga atatattggt
     2581 gaagatggct attattcgat ggtttttgat tttagttata cggatattga tgtacctgaa
     2641 actggtgaat ggtttaaaga tagccaatgg acatggacgg atatgcgtac caatattttt
     2701 acgaatcaat tagttacgca agataaaggt tggggcgcct tatatttaga aaaccacgat
     2761 caaccacgtt caattaataa atatattcca gaagaatgga tcaatgatta tagtaaaaaa
     2821 atgttggcga cgctttttat gttactaaga ggtacccctt ttatttatca aggacaagaa
     2881 ctaggaatga ccaacataga aatggcatca ttagaagatt ttgatgatgt agctacgcat
     2941 tctcagtata aacgggcatt agaatatgga ttggcaccag agcaagcatt gaaagctgtc
     3001 aataaacgaa gtcgggataa ttctcgcaca cctatgcaat ggaatacgca aaaaaatgcc
     3061 agattctcaa cttctgataa tgtatggtta aaagtaaatc ctaattatcc agaactcaat
     3121 gcagaagctc agttaaccaa tcaacagtcg gtattcaatt attatcatca attaattaaa
     3181 ttaagaaaaa atagtgtata caaagacgtg ctaatctacg gacaatttct accagtgaaa
     3241 gataaaaatg aacatatttt gctttatgaa cgccaattag aagataaaat cgtattagtt
     3301 atcttaaacg ttacagcaac agaacaagaa tattatgtag atgaacagta ccaacaagta
     3361 ttaatcaata attatgaaga tgttttatta gatcaaagtc gaaaattgcg tttacgaccg
     3421 tttgaaagta ttgtattagc taattatgga ggacaattat gaaaattaaa aacgaagcaa
     3481 tgctcatcac ctatgcagat agtttaggaa aaaacatggg cgaattaaaa caagtcttag
     3541 acaaacattt acaaggtgtt attggtggtg tacatctgtt gccttttttt ccatcgacag
     3601 gtgatcgagg ctttgcgccg agtgattata cgacagttga tccagctctt ggaacatggg
     3661 aagatgtgga agcattaggt gaagattatt atttgatgtt tgattttatg attaatcaca
     3721 tttctcgtga atcaaaattt ttccaagatt tcaaaaaaaa ccatgagcag tcaccataca
     3781 aagaaatgtt tatccgtatt catgaatttt tcccagaaaa tcgaccaaca caagaagata
     3841 ttgatttaat ctacaagcgt aaagacaaag cgccgtttca aacggtagaa tttgcggatg
     3901 gtacgacaga agaggtttgg aatacctttg gggaagaaca aattgattta gatgtgacca
     3961 aagaagttgt gaaagaattt attcgagaaa ccatcaaaga tatggcgaac catggctgtt
     4021 ctttaattcg attagatgcg tttgcttatg cgattaaaaa attagatacc aatgatttct
     4081 tcgtggaacc agatatttgg gaacttttag acgaagtgcg tcaagaagca gcgaaatatg
     4141 acgtcgaatt attaccagaa atccatgagc attattccat tcaaatgaaa attgccaatc
     4201 atgattatta tatctatgac ttcgccttgc caatggtcac tctgtattca ctttatagtg
     4261 gaaaatcaga acgtttagcc aattggttga aaatgagtcc gatgaagcag tttacaacat
     4321 tggatacgca tgatggaatt ggtgttgtgg acgctcgtga tttattaaca gatgaagaat
     4381 tggattacac ctctgaggaa ttgtataaag tcggggcgaa tgtgaagaag gtctattcaa
     4441 gtgccaatta caataattta gatatttatc aaattaacag cacctattat agtgcattag
     4501 gcgataatga ccgtagctat ctgttagcac gtgcgattca agtatttgcg ccagggattc
     4561 cacaaattta ttacgttggg ttattagcag gaaaaaatga tattgaactg ttggaagcaa
     4621 cgaaagaagg gcgtaatatt aatcgtcatt actatgattt agccgaaatt gaagaagaag
     4681 tgcaacggcc agtgattcaa caattatttg cacttttaaa atttagaaat gaatctgcgg
     4741 cgtttgattt ggatggaacg attgaagtag caacgccatc agaatcagaa atagtgatta
     4801 cacgtaaaaa taaaaacggt agtgatagtg caacattaac ggctaacttg gatacgaaag
     4861 aatttatcat tgagtctaaa gatataaaaa ttttcactag tgttgcatta aaattttaac
     4921 ctattccata gaacaaaaaa tcctttatac tagcacttgg atgacttatc caagaccaat
     4981 aaaaaggact catacatgga taagtataaa acaaatacag catttaataa atggttttct
     5041 gccataaaat taggaaaact ttctgtcaac tcccaaaaat gtatttttga ttacaattcg
     5101 tatcgtaaaa aactgtcttt tgaagctgtg ctcaagctct ttctctacgc agttaatgaa
     5161 gaaaaagaaa gtttgcgtga tttgagtaca gctcttatcg accgatcgct ccaactagaa
     5221 accgatgtgt catcgattag tcatacccaa ttgtctcgag catttaatgc acttgaacct
     5281 aaagtgttag aagaaatttt tcaacaactg ttagaaaaag ttcaacatca aatcaaacca
     5341 accaagcgaa atcatctgta tctgattgat tccagtacgt tttcgcttag tttaaaacgt
     5401 cataagtggg cgactttccg tcaaacaaaa tcaggtgtca agttacactt aaactattgt
     5461 tatatggacg atactacgat gtatccaact gattttacct taacaaatgc ccatgaacat
     5521 gatctcaatc aactaccagt attggtgaat caaccagagg caacttacgt gtttgatcgt
     5581 gggtattagg actttgaaaa gatggatcag atgcattggg atggctattt cttcgtcact
     5641 cggattaaaa aaaacaccaa agttcacgtg atagatactc tggaaacatc gccagaaaca
     5701 gaaattctac gagacgagct ggttcgttta ggatctaaaa cctatctaac cgccaatttc
     5761 cgactagtca ctgttcaaga taagaacgga cgtgtgtttc aatttatcac caatcgaatg
     5821 gacgtctctt caaaagaaat ctctgatatg tatcacgctc gttggcaaat cgaattgttt
     5881 ttcaagcaca tcaaacaaca catgaccatt aaaaccttct tttctcaaag tgaaaaaggc
     5941 gtgcagaatc aattgatttt aacgatgatt tccgctctcc tcacgtttct catcaaattg
     6001 gagacgaaaa cggaaaaatc agtgttccaa atcaaacgat tctttcgtta tttactgttt
     6061 cagccctttg aatgttgtct agaaaaatta attcccacat gagaaaaaag aaatttggct
     6121 cgttactttt ggttttcagt tgaataacag ataaaaaatt ttctggaaaa agtcatcctc
     6181 tgcgtcagca cagttttttt tgacttattt tcagaggaaa tagttttcag aatattctaa
     6241 aatgttttat gcaacactag tgatattttt tagaattaga tttctaatgt gcattttttg
     6301 agtattaagg agcaagtata caaagattta taaagctgat taaatttgaa taaaaatatg
     6361 caggaacgct actattttta gtagtgttct tttttatatt tgaataaaaa atgtggtggt
     6421 taaatggtgg ttataacaaa aaatgaatta atgaaattag gttatgggaa gtatcagtct
     6481 gagagaatca tacgacaggc aaaagcaatt atggtttcaa aaagctatac ctattacgaa
     6541 aatagaagat taggactagt acctgttgaa tctgtagaag aagttttagg tatgaagata
     6601 gaggtagagg aggattttca gaatcatggt taattttaaa aaagataaaa atgggaaata
     6661 tcattttact gctagtttgg gattcgacaa acgaacaggg aaaagagtac aaaaaaacgt
     6721 agaggggtta aaacagttaa agaagaaaaa aagcgtatac cgaacttgtt tctaattatg
     6781 gaaaagaagc agaaacttct caaagtacta tgttgtttga ggagtttttt tataaattat
     6841 tcctacctta ttatcaaggg aaagtagaag aaagaacata taaaaatcgg ttatctacaa
     6901 tcaaaaaaac ttttcttatt tctttaaaat ggagttaaga gaaatacgtc caattgacat
     6961 tcaaaagtgg caaaataatt tattgagcaa atataaaaat tcttatgtaa gaaacatgta
     7021 tgggattttt cagatgtcat tagatcgagc agttgttttg ggactacttg aaaaaaatcc
     7081 tgcaaaagtt attggaaatg tgaagaaaag gaaaactaca gttgattttt ggacaaaaga
     7141 agaatttaca aaagttattt caacttttta tattgatgat ttgtaccaac attttggttt
     7201 tatttgtatg tggttacttt ttatgaccgt tatgcgtttg ggagaagcca ctgcattaac
     7261 ttggaaagat attgattttg aaaataaaaa cttgcgtgtg aataaaacac tctattataa
     7321 gagagccaaa acctatgagt ttgtagaacc cgaaacggct tctagtattc gtacaatcga
     7381 actagacgac aatacaatta cctttttaaa agcttggaag gagcgtcaag tttcaatagg
     7441 aggcatgtcg tttgtacttt cttataatgg aacacctact cagaaatata caatccgtca
     7501 tattatgaga agacatgcaa cgatcgttgg tgttcatata atccgcatac acggattaag
     7561 gcatagtcat gcttctttgc ttatatcaca ataaaaagat gcacttcgaa ttaaagaaag
     7621 gttaaaacat gctgatgtac aaacgacttt aggaacttat ggtcatttgt atctaaattc
     7681 attgagagag ataacaaatg atcccacaga ttatatccaa actaattttt ctaaaatcaa
     7741 accgacacct atttacgata ataaaaaata aatgataaac aaaagtagga tcacaccaaa
     7801 agaaacacac tagaaaaact ataatacagg cttttctagc gtgttgtttc ttatttccgc
     7861 ctatttatac attatactca taaagcattt aatgcatcga tcgtagccgc attgtctata
     7921 tgggtatata aagttgttgt attggtactt gaatgtccca attgttgggc agttaatacc
     7981 aatgaatttg tctgttggta gagtctagta gctaatgtat gacgcagttt gtgagggctg
     8041 acttgaattc taaagccttt tgaatatttt gctaccatcc gttcgattgc aataccggat
     8101 atacgcttgg cttctccttg gtaaattgct agaaataaag ctttttcgtt ttctgttact
     8161 ttatatcgtt ctttccgaat agctagataa ttagctaaat attccattgc gaatgctgct
     8221 atattgacag catctctttt atttcctttt ctagttacta ccacattatt cttatctaaa
     8281 gataggtcat ctaaattaat atttataacc tcggagagtc tcaatccaga tcctaaaatc
     8341 agtgcattga tcgccaggtc tctttctaca tctcgttgat aatagatcaa agctcgttta
     8401 ctcaaagtat ttttatattc attggcaata taatctagat aattttgtgt atccttatct
     8461 agaaataatt ttttctcaat ggcattggca cgtgcagcta acgtagtttt atctgtgatg
     8521 gttgctactt gatccatagg attattgtct aagtatggtt ttcctagagg attattggaa
     8581 cttttcgaaa gatatttaaa tagaaccttt aatgccgtga tatttctatt gatagtagaa
     8641 gtagatagac ctaagttatt attatctttg agcttagttt ttgttgtctc tttttttaca
     8701 ttttcttttg tgcgacgttt caaatgagat ttgtacaagt ttaggtcata ggcagttaac
     8761 tgtcttatcg aggtgataga agtatcagga acgatagact cactcagaag ccattcataa
     8821 aagagcttat aattttttag atattctaaa gtggttcgag gtgattgatc tttatcctca
     8881 cgatcttgaa
//
LOCUS       DACDMM010000109         8870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDMM010000109 DACDMM010000000
VERSION     DACDMM010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.110"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            144..2741
                     /gene="adhE"
                     /locus_tag="IWD16_002029"
                     /gene_synonym="adhC"
     CDS             144..2741
                     /gene="adhE"
                     /locus_tag="IWD16_002029"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP9089033.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2970..4076
                     /locus_tag="IWD16_002030"
     CDS             2970..4076
                     /locus_tag="IWD16_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9089034.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4093..5121
                     /locus_tag="IWD16_002031"
     CDS             4093..5121
                     /locus_tag="IWD16_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP9089035.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5127..5606
                     /locus_tag="IWD16_002032"
     CDS             5127..5606
                     /locus_tag="IWD16_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP9089036.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5622..6917
                     /locus_tag="IWD16_002033"
     CDS             5622..6917
                     /locus_tag="IWD16_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP9089037.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6928..7752
                     /locus_tag="IWD16_002034"
     CDS             6928..7752
                     /locus_tag="IWD16_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9089038.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7917..8816
                     /locus_tag="IWD16_002035"
     CDS             7917..8816
                     /locus_tag="IWD16_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9089039.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
BASE COUNT         2849 a         1539 c         1967 g         2515 t
ORIGIN      
        1 aaacacctgt aaataaagca attttatatt taaaaataac tgtgaaaaaa atcacaaaaa
       61 agtgtttaca tagaaaaata aatattgtat gatatacatg tgaattattt aacaaataac
      121 catgacccat taggaggata atgatggcta aagtaataga aaaagaaaaa acgaagacaa
      181 ttgacgtaca agcaatgatt gatgatttgg cagaaaaagc aaatgcagca ttgaaagaaa
      241 tggaaagctt tgatcaagaa aaagtagacc atatcgtgca tgaaatggca atggcagctt
      301 tggatcaaca tatgccatta gcgaagatgg ctgtagagga aacaggccgc ggtgtttacg
      361 aagataaagc aatcaaaaat atgtatgcgt ccgaatatat ttggcataat atcaaacatg
      421 ataaaacagt tggtgtgatc aatgaagatg tccaaaaagg gttgattgaa attgctgaac
      481 cagtcggtgt ggtttgcgga gtaactccaa caacgaatcc gacttccaca acgattttca
      541 aatcaatgat cgcgttgaaa acaagaaatc caatcgtttt tgctttccat ccaagtgccc
      601 aaaaatcttc tgcagaagca gcacgagtgg tacgtgatgc tgctatcgct gccggagctc
      661 ctgaaaattg tatccaatgg atcgaacatc cttcgatcga agcgacttct atgctaatga
      721 accatccagg tatcgcgatc gtcttagcga caggtggagc tggaatggtc aagtctgctt
      781 actctacagg aaaaccagct ttaggtgtag gtccaggtaa cgtaccagct tatattgaaa
      841 aaacagcaaa aattaaacgt gcagtgaacg atttgatcgt ttcgaaaaca tttgataatg
      901 gaatgatttg tgcttctgaa caagctgtga tcgtggataa agaaatctat gcagcagtaa
      961 aagcagaatt ccaagcacac caagtttata tcgtaaaacc agatgaattg caaaaattag
     1021 aagatgcagt gatgaacgaa ggaaaatatg cggtcaatcc atcaatcgtt ggacattctg
     1081 cgatggaaat cgcaaaattg gcaggaatca gtgtaccaaa aggaacgaaa atgctgattg
     1141 ctgaattgga aggcgtaggc ccagattatc cgctatcaag agaaaaactt tctccagttc
     1201 ttgccatgat caaagctaac aacacggacc atgcgtttga tttgtgtgaa ggtatgttga
     1261 atcttggagg attaggacat acagcagtta tccattcaga aaacgaagag ttgcatgtca
     1321 aattcggtct tcgaatgaaa gcttgtcgta ttttagtgaa tacaccatct gcagaaggcg
     1381 gcatcggtga tatctataac gaaatgattc cgtcattgac tttgggatgc ggttcttatg
     1441 gtaagaactc tgtttctcgt aacgtctctg ctgtaaactt aatcaatgtc aaaactgtag
     1501 cgaaacggag aaataacatg caatggttca aattaccgcc aaaaatcttc tttgaaaaga
     1561 actctctatt gtacttagag aaaatggaaa acgttgaacg tgtcatgatc gtttgtgacc
     1621 ctggtatggt ccaattcggt tactgcgata cagttcgtga agtattggca cgacgtaaaa
     1681 atgatgtgaa gatcgaagtc ttctcagatg ttgaaccgaa cccatcgaca aataccgtat
     1741 acgcaggaac gaaactgatg gcagacttca agccagatac agtaatcgct cttggaggcg
     1801 gttctgcaat ggacgcagct aagggaatgt ggatgttcta tgaacaccca gacacatctt
     1861 tctttggtgc aaaacaaaaa ttcttggata tccgtaaacg gacatataag atcgacaaac
     1921 cagaaaaaac acaattcgta tgtatcccga caacatcagg gactggttct gaagtaacgc
     1981 catttgcggt catcactgac agtgaaacac atgtgaaata tccgttagca gattatgcat
     2041 tgacgccaga tgtcgcaatc gtcgatcctc aattcgtcat gtcagttcca gcatctgtca
     2101 cagcagatac cgggatggat gtgttaacac atgcaatcga atcctatgtt tcagtcatgg
     2161 cttcagatta tacacgtgga ttgagcctac aagcaatcaa gttagtcttt gactatttag
     2221 aaaaatcagt aaaaacacct gatatggaat cacgtgaaaa aatgcacaac gcttctacaa
     2281 tggctggtat ggcgtttgct aacgcattcc taggtatttg tcactcagtc gcacataaaa
     2341 tcggcgggga atacgggatc cctcatggac ggacaaatgc gattctttta ccgcatatta
     2401 tccgctataa tgcaaaagat ccacaaaaac atgcgatgtt ccctaaatat gactatttcc
     2461 gtgcagatac agattacgca gatatcgcaa aattcttagg tctgaaaggc gaaacaacag
     2521 aggaattagt cgaagcttta gcaacggctg tttatgaatt aggaaaatct gtcggcatca
     2581 acatgagtct aaaagctcaa ggagtcacac aagaaacact agatacaacc gtagaccgta
     2641 tggctgaatt agcttacgaa gaccaatgta cgacagctaa tccgaaagaa ccattgatct
     2701 ctgaattgaa acagatcatc attgatgctt acaacggata agcaaaagaa agcgtagaaa
     2761 aaagataaga aaatagatga aaaagaactt ggaaaacgtt gtttctccaa gttctttttt
     2821 taatttccaa gtaactgaaa gttacttttg ttatttaaca tttaggtcaa ttttagacag
     2881 tttttgttca aaaagcgata tttatagtgg acggtattac tgctatatta aggatataaa
     2941 atgaaagcgt ttaaaaaggt ggcgtaaaga tgattattaa agaagagatt tttgtaccta
     3001 aagaattagt gaaagaaaaa attgacctgc tcgttgaaaa cctgacaacg atcaaagacg
     3061 ataatggtga atttttgttg gactttgatg gactgaaagt agatgacaaa agctggacag
     3121 tgtggaattg gccacaaggt gtcggtctat atggtattta taaaaattac cagatgacaa
     3181 aaaatcctcg cgcttatcaa gtggtcaatg aatggtttga ggatcgtatg gaagaaggcg
     3241 ctccgcccaa aaacgtcaat acaatggcac cgctattgac tatggcttac ttgtatgaag
     3301 acacgaaaga ttcacgctat ttgccttatt tagaacaatg ggcagaatgg gtcatgaatg
     3361 acatgcctcg gacaaatgaa gacgggttac agcatgccac atatggaccg gaaaataaga
     3421 atcagttgtg ggacgataca ttgatgatga cagtgcttcc tttggcaaaa atcgggaaac
     3481 tgttgaatcg cccagaatat ttagaagaag ctcgttacca gtttatgatc catatcaaat
     3541 acttgatgga taaacgaaca ggattgtggt atcacggctg gacgtttgaa ggaaatcata
     3601 actatgccga agcattttgg gctcgtggaa actgctggtt gacgattgct attccagaga
     3661 tcattgaaat cttagaatta tcgaaagaag atgcattgcg caaattattg atcgaaacac
     3721 ttgaagcaca agtgcgtgca ttaaaaactt atcagtctga aagcggttta tggcatacgt
     3781 tgttggatga tccttcttct tacgtagaat cttctgcaac tgcaggattt gcttacggta
     3841 ttttgaaagc ggtccataaa cgttatttac cacaagaata taaagaagtc gcttatcgtg
     3901 ctatccaagg attgcttgaa caaatcgatg aaaaaggaga agttcaaaac gtttcaatcg
     3961 ggacaggaat gggggacagt ttagattttt atcggaatat cggaatcaca gcaatgcctt
     4021 atggacagtc gttaacggtg ctttgtttga cagaattgct cgtctcttat tgttaaaaga
     4081 aaggaaaccc atatgaaaaa aacatcactg ctgtttgcag caagttcgct tagcctaggt
     4141 ctgctaggtc taagcggctg cggaaataat tctagtgtgg caactgtcgc agatgcagat
     4201 gtaaatgaga tagttacctt gcgctttgct tatgcaagca acagccagcc agtaattgat
     4261 gcaatgaatg aatttgggcg tttagtaaaa gaaaaaacag atggttccgt acagatccag
     4321 tatttcccag atggacaact gggtggagaa cgtgaactga tcgaattgac acagtcagga
     4381 gcagtagatt tcacaaaagt aagtgcctca gcgttggaaa gtttctctaa agattattcg
     4441 attttttcag ttccttatct attcaatgat gaagaacatt tctttaaggt gatggataat
     4501 gaagaaataa tggagccaat ctaccaatcg accaaaaatt taggatttac cggattgacc
     4561 tattatgatt ctggtcagcg cagcttttat atggtagacg gtccgatcca tacgccggaa
     4621 gatttgaagg gaaagaagat ccgtgtgatg caaagcgaaa ccgctatcaa aatggtagag
     4681 cttttaggcg gttcgcccgt acctatggga agtgatgaag tgtacacgtc tcttcaatcc
     4741 aatttgatca acggatcaga aaacaatgaa tttgtcttgt atacagctgg acatggtggt
     4801 gtggctaaat actattccta tgatgagcat acacgtgtgc cagatatcat catcatgaat
     4861 gatgccatca aagaacagct aactgatgag caggaaaaag ccattgaaga agcagcgaaa
     4921 gaatcaacgg tttttgaaat tgaagctttt gcaaaagcaa ttgaagaaga aaaagcttta
     4981 gcacaagaag aacatggagt ccaattcaat gatgtagata atacaccgtt tcgagaggca
     5041 gttgccccac ttcatgatat attcaaaaac gatcctacct acaaagacct ttatcaaaag
     5101 atccaagagc aaggagcgta gctgtgatga aaatgatttt aaacaaggta ttagagatcc
     5161 tgggagctgt tttgcttgtt gggatggtcg ctgttgtttt atggcaagta ttttccagaa
     5221 cggttttagg aaatccaaat acggtcacgg aagagcttgt tcgattcggt cttgtctggt
     5281 ttgccatgtt gtcttccgcg tatgttgtgg gacaaaaagc acatttagcc gtaacggttt
     5341 taagtgaaaa attaactggc aaaaaaagta aaattttaga agttgttgtt caaagtttat
     5401 ttctggtatt tgctgcgatg atcatggtgt atggcggatg gaatgctgtc acattgacaa
     5461 tggggcagat ctcgccttct ttaagtattc ctatgggata tgtttatctg tcagtacctg
     5521 tatcgggggt actcatcatt atctatagcc tgatcaatct aatcgataca ttaagcaaca
     5581 agccagcaat tcaagaaaaa atataaaaat ggaggaataa aatggcaata gaagccggat
     5641 tagtgttatt ttttgtattt gttgtgttgc ttgtattagg gatgccgatt gctataagta
     5701 ttgcattctc atcgatgatg actttattgt tagttatacc gtttgatgtt tctgctttta
     5761 gttcagctca aaaaatggtg ggaagtatca atagtttttc gctgatcgct attccgtttt
     5821 tcgttctctc gggcattatc atgaataatg gagggatagc taagaaacta gtggattttg
     5881 cgatgttgtt cgtcgggcgc gtgccggggg ctttggctca tacgaatgtg ttagggaatg
     5941 ctttatttgg ttccatctct agctcggcca ttgccgcttc taccgcgatt ggcggagtgt
     6001 tgatccctca gcaagtggaa gaaggatatg accggaagtt tgcgacagcg gtcaatatcg
     6061 cgtcagctcc gacaggtatg gtcattcctc caagtactgc ttttatcatg tattcacttg
     6121 ttgctggtgg tgcttcgatc tctgctttgt ttatgggagg ttatctcgta ggcgctttgt
     6181 gggcgctagg aatcatgcta gtagcattca tcgttgctaa aaagaataaa taccctgttg
     6241 tcgaaaaagg acagatggct catgctggaa aaataataaa agaggcgatt cctagccttc
     6301 tattgattgt catcattatc ggtggtatct tgacggggat atttacagca gtagaagctt
     6361 cagcgatagc tgtcatttac tccctgttga ttgctatgtt ttactataaa acggtaaaac
     6421 taaaagatat acctaatatg ttgtttcaat cagtagcaat gtcagggacg atcatgttct
     6481 tattggctac ttcttccatg atgtctttcg ccatggcgtt taccggtatt cctcaggcaa
     6541 ttagttcgtt gattatgggc gtaacggata ataaatttat tattctgtta ttagtcaatc
     6601 ttgtgttatt actggtgggt atgtttatgg atgtggcgcc tgctatcttg atttttacac
     6661 caatattttt accaatcgtc acaagtgtag gggttgatcc tgtacattac gggttgtttt
     6721 caatcatgaa tctgtgtgta ggctccatca cgccgccagt aggtacagga ctatatgtcg
     6781 gtgcaagtgt aggaggcgta aaagcagagg caatgctgaa accgcttctt cctttttatg
     6841 gggttatctt agtcgtcttg tttttgatta cttacttccc gcaaatcgtc atgtggctac
     6901 ccaatatgct gagttaggag aatagttatg ttgaacatta caattagagg acatgatctt
     6961 tccgaagtcc aaacgataga agatcttgca gaaaaaacaa aagaacaagg gattcataca
     7021 ctgcagctcg ccttaggtct ttcgtttcct gaaatacctt ctggcgcaaa tgagatcaat
     7081 tctgggatgg gaaattatgt caaacgagtt ttagaaaagc aagaagtgac cgtaggaatt
     7141 ttaagttgct atatcaatat gattcatcca gatttaacga tacgacaaga gttgctgaca
     7201 aagttcgagc agtatcttcg ctatgcttct tcctttggtg cgtcgatggt tgcttcggaa
     7261 actggatgtg ttttgccgga aatccaatat acggaagaaa atttcacaga tgaagcattt
     7321 gctgaagctg tgtccgttat ccgtcgcttg gtaaaagctg gagaaaaata ccaaatgatg
     7381 gtggggatag aaccaggttt gaatcatccg gtgtattctt tagcaagggt agagcagctg
     7441 atccaagcag tagattcgga ctatctagga atcattttag atccgaccaa tttgatcaca
     7501 agtagtgatt atcaagaaca agtccaattg gtagaagaag cttttgaacg ttttggtgaa
     7561 aagatatgtg cggttcattt gaaagatttt cgtgtcgaac aggaaaaaat cgtgccggta
     7621 aatttgggag acggtgtgat cgagtacaca aaaatcaaag aaatcatcat gaagaatcgt
     7681 ccgtacctgt atgtggttct tgaagaaaca aaagatgatg ggattcgtta tggaagaagt
     7741 cttcttgaat aatgtaaaga agacgttggt taactaatat ttagaatgga gggggcgggg
     7801 ctagaagatg ccacgtctct tcttttttat aagagtatat aaaagcagct ctttttgatt
     7861 acaaaaaacg tggtataata aaattgtgca taagtgaaca aaaggaggtg cggcacatgc
     7921 tccaaaagaa aatcagcggt gaacaagtca atacgatggt ttctgatcag tatgaaatct
     7981 atcatgtcaa agatgtcgtc agccaaaata aaacgatcta tcatcatcat gatttctatg
     8041 agatccatgc tacattaaag ggagaagcga ctttttattt gaacggacga caatttacga
     8101 tcaaagcagg aagtgtgctg ctcatccatt ccaatgattt gcatcgtatc gtccgccaat
     8161 cttcagatgt attcgagcgt gtctatatct ttgtcacccc ttcttttcta gaaagtcgtt
     8221 ccacgccatg gtctaatcta tcggcttgtt ttcaaccaat gggagagaag aggagtcgtg
     8281 tattgcaaat agaacaggaa gtattgaagc aaaaattatc atttatcgac catcctcctg
     8341 ataaaaaaat ttacggtgaa gacataaaat atgaacaagc tctggtagaa tacttgattt
     8401 ttctcaatca agttgtacaa aaagaagaga gtgcgcaaga acagaatgct gtagttccta
     8461 atgagcgtgt ggaacaaatg ttacaatata tctcaaaaaa tttatctcat ccgctttctt
     8521 tagaggaaat ggaaaagcag ttttttgtca gcaaatacca tatcacacga gaatttaaaa
     8581 agtacactgg atttacgttt catcagtatg taatgaagaa aaagctgatc tatgccaagc
     8641 acttgttgcg agaataccgc agctctagtg ctgtatatag taaaagtggt ttttcttctt
     8701 atccgcattt tctgaaagca ttcaagagag agttcggcat aacgcctaaa gaatttttga
     8761 aacgtgatac acagaaacag ttggttcatt atgaacattt tgaagatgaa caatgagaat
     8821 gatcactaaa aaataaccgc agtagctgct gttgtttaca tagaagcaga
//
LOCUS       DACDMM010000110         8712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDMM010000110 DACDMM010000000
VERSION     DACDMM010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.111"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            45..524
                     /locus_tag="IWD16_002036"
     CDS             45..524
                     /locus_tag="IWD16_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP9089040.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            741..1832
                     /gene="phnW"
                     /locus_tag="IWD16_002037"
     CDS             741..1832
                     /gene="phnW"
                     /locus_tag="IWD16_002037"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP9089041.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            1825..1953
                     /locus_tag="IWD16_002038"
     CDS             1825..1953
                     /locus_tag="IWD16_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9089042.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            1957..2502
                     /locus_tag="IWD16_002039"
     CDS             1957..2502
                     /locus_tag="IWD16_002039"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9089043.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(2956..4647)
                     /locus_tag="IWD16_002040"
     CDS             complement(2956..4647)
                     /locus_tag="IWD16_002040"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP9089044.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(5067..6014)
                     /gene="arcC"
                     /locus_tag="IWD16_002041"
     CDS             complement(5067..6014)
                     /gene="arcC"
                     /locus_tag="IWD16_002041"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP9089045.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(6129..7148)
                     /gene="argF"
                     /locus_tag="IWD16_002042"
     CDS             complement(6129..7148)
                     /gene="argF"
                     /locus_tag="IWD16_002042"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP9089046.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(7239..8468)
                     /gene="arcA"
                     /locus_tag="IWD16_002043"
     CDS             complement(7239..8468)
                     /gene="arcA"
                     /locus_tag="IWD16_002043"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP9089047.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         2515 a         1752 c         1593 g         2852 t
ORIGIN      
        1 ttttgttatg atacatgtga aatctttcgt aaaggagaaa gaaaatgcgc aaagctcaaa
       61 gacatttgct gattaaacaa gtcattgaag aattcccgat ccgtacgcaa gaagaactat
      121 taaacaaact tgggcattat ggcgttaccg ccacgcaggc aacgatttct cgagatatcc
      181 gtgatctaaa aatcgtgaag gcacctgatg aaaatggcgt ttcccgcttc gtattgtttc
      241 gcgaaaaagg aactcctgag actaggactg aggaagagga tcggctggtt caaatgattg
      301 aagacattgt attgaaagtc gaacgagtcc actttttgac gatcgtcaat accttgccag
      361 acaatgctca tttatttgca tctgttttag atgagatcaa acctccctct attgtcagta
      421 caattgctgg attcgatacg atcatggtca tctcggaaaa tgaggaggac gcaaaacgag
      481 tagaagaatt tcttcaccat ccaactgaag tatcactttt ttaatcagag cacgtattcc
      541 taatatggaa tgcgtgtttt tttattttca atattttcct tattgaataa acctttacac
      601 tctttacaaa aatacatcat tttttattca aatttttcaa caccttgatt gaatcacaat
      661 aatcatcatt tatattggat gttaacaaga gcagcttctt gctctttgtg tatttcaaaa
      721 aagaaatgaa aggaaatcat atgtataaat tattaacttc tggaccattg acgacttctg
      781 aaacagtgaa aaatgctatg ctgatcgatc attgtacttg ggatcaagaa tacaaagaaa
      841 ttacacaatg gataagaaaa aaattattgg atcttgctca agtatcggaa ggaatataca
      901 caacggtctt aatgcaaggt agcagctcct tcggggtaga atcggtatta agttctgtga
      961 tcaggccaga agatactgta ctgatttgtt caaacggcgc atatagacaa cgaatgatca
     1021 acatgtgcga atgtctgaaa ctgaactata ccgtttatga ggtatcggaa catcaaatcc
     1081 tccaagctca tgtgattaca cagctgattg aagagaatcc gtctatctcc gcactagcaa
     1141 tggtccatag cgagactact tccggtattt tgaataattt ggaagagatt gctgctgtca
     1201 cacaagaaag agatcttttg tttatagtgg acgcgatgtc aagctttggt gggataccga
     1261 tttgtgtagc cgaactaggg attgattttt tgatttccag ctcaaacaaa tgtattcaag
     1321 gtgttccagg cttttctttt gtacttgcta ataaagcgga actggaaaaa cgaaaaaatg
     1381 ttagccgaag tttgtcactt gatctatatg accagtaccg cgtgatggaa aacgagaatg
     1441 gcaaatggcg tttcacttcc cctacccata cggttttggc ttttcaacaa gcgctgaagg
     1501 aactagaggc agaaggagga atcgaaactc gatattcacg ttatctcgcc aataatcatg
     1561 tgctacgaca aaaaatgaca gagatggggt tccagccttt cattcaagaa tatcaggggc
     1621 cattcatcac ctcttttctt taccctaagg actggcgatt tgattttaat catttttatc
     1681 agtatttaaa gcaaaatgga tttgtgatct atcccgggaa aatcagcgat catgaatgtt
     1741 ttcgtatcgg aaatattggc gagatctatc cggaagatat tttggaactg acagcaatta
     1801 tcaaagacta taaggagaac gttaatgatt aaaactgtaa tttttgattg ggcaggaaca
     1861 actgtggact ttggctgtat ggctcctgtc cacgctttta gaaatgcttt tctagaaaaa
     1921 ggaatccaac tgactgacaa agaaatccgc taaccgatgg gaaaattaaa atgggatcat
     1981 atccaacagc ttctttccct cccttctgtc aaaaaacaat ggatagctat ttatggacaa
     2041 ctacctcagg aaaaagaggt ggaggaattg tatcaacaat ttgaacatta tctgtttaaa
     2101 gatttggtca agcattctac cttgaaaccc gacacattgg aaacgatcga acggttgaga
     2161 aacgctggga tcaatattgg ttccacgaat ggatttaatg ctcctatgat gacaatcgta
     2221 gcagaaaccg caaaaaaaga aaggtattca ccagacttca tggcaacttt tgaagacgtc
     2281 gaagggtatg gtcgaccgtt tccttatatg atccataaaa acatgcagta cttcaaaaac
     2341 aagtcggttg atgaagtaat caaagtcgga gatacggtct cagatatcca ggaagggaaa
     2401 aatgccggag tgttgacagt aggtgtaatt gaaggtagtt cgctaatggg gatttcttat
     2461 gatgagtacc aaaaattaga gatggggaaa aagatcgttt aaaacagtct gttgctctta
     2521 gattccaaca agccggcgct gatatttgtg taaacaattt aaaagagtta acagaataca
     2581 ttttgtacgc tgacatgaat ccagaatgac ttttatacaa aataagaggt tgtgaaaaag
     2641 ctgtcctgta tcaagtaata aaaacagcca agcaaaaata gctcctcata tttttcgttt
     2701 ggttaggctt attaccgcag atgcaagctt ttgaacaccg tttattcgaa aaaaaaaagg
     2761 gagctgtgac acgacttttg tcacagtccc cttatttgca aacaaacagt gtttaagaga
     2821 caacctctcc actaaacggt gttcattcag ctgactctcg gtatttgctc aaaaaacgat
     2881 aaaatttgaa aagctatttt ctcttttgtt tctctaatac tcggatcaga acgctgattt
     2941 cacattctct ttttcttaca tcttatctgg tgcatgaagt cctagcaaac gaaggtcttc
     3001 tttcaataat acagtcactg cataaactag agctaagcgt gcttcttttt gttcatcatc
     3061 agctaaaatc ttcgtatgag catagtattt gttgaatgct tgtgctaatt tgattgcatg
     3121 tttcgcaatc actgacggtt cgtatttttc cccagcagac aagaccgttt cagggtattt
     3181 ttgaactaat ttaaccactt cccagctggt atcgtcattc aatgcatagt ctgctgtttc
     3241 tgaagggaca aatcctgctt tttccaacag gctgactgca cgagcatgtg tatattgcac
     3301 ataaggtcca gtttctcctt caaatcgaac aacttcttcc aggttaaagt caaatgtatt
     3361 cagtcgatcg tttttcaaat cgtggaaaat gactgcccca accccaactt gtttagctac
     3421 agcatctttg ttttccaaat cagggttttt ctcgctgatt tgttctttag cagattggat
     3481 tgcttcattt aatacttctt ctaataagac gatctttcct ttacgagttg ataatttttt
     3541 accaccttgc gtgatcaaac caaatggaat atggtgcatg tcgtcagacc agtcaaagcc
     3601 catttccttc aagacagctt tcaactgttt aaaatgatag ctttgttcat tccctactac
     3661 atatagagat tgtttgaagt cgtaagtacg tttacggtac aatgcagcag ctaagtcacg
     3721 tgtaatgtat agtgtcgcac cgtctgactt cttaatcaac gctggattca ggtcataagc
     3781 agataggtca acgatctctg cacctttatc ctcgttcagc aaatgttttt cttctaaaag
     3841 tttgacgatt tcatccatct tgtcattgta gaaagcttca ccgttcaacg aatcgaaacg
     3901 tacttccagc agatcataga ttttattgaa ttctttcatc gattcatcac ggaaccattg
     3961 ccataattga atagcctctt tatcgccttc ttccagacgt ttgaaccaag cacgagcttc
     4021 ttcatttaat tctggttctg tttcagctac ttcatggaat tggacgtata aacgaagcaa
     4081 ttcattgatt ggttctgctt taacagcttc ttctgtaccc cattttttgt aagcgacgat
     4141 cagttttcca aactgtgttc cccagtcacc taaatggttg atgcggattg gctgataacc
     4201 gattttttcc ataatgaaac cgatcgaatt accgataact gtcgaacgca aatgtcccat
     4261 tgagattggt ttggcaatat taggagaaga catgtcgatt ggtactgttc cttgattccc
     4321 gatgttgcta tccccataat gttctttttc tttcactact gtttgcaaaa cttttttgct
     4381 gataagttct ttattcatga aaaagttcaa gtaaggtccg acaacttcga ttttttcaaa
     4441 gttggcagaa tcgatttttt ctgctaaatc tgcagcgatt tgctgtggcg cttttcggta
     4501 aacttttgct aatgaaaagg ctggaaatgc aacatctccg tgttcagctg attttggatt
     4561 ttctagtaat tgctcgactt gttccagtgt taaatcatct ttgacagcgt catagacagc
     4621 ttttgcgaca agatctttat tattcatctt tattcctcct aaagtgttcc cttgattgtt
     4681 aaacaaaaaa aatctctagc gacggcatac ttagtatgcc atcactagag acgagatttc
     4741 ccgcggtacc actccaattg tcctaaaaaa ggaccagctc catctgatat cggtagacga
     4801 ccgtctgttt aaaacagaat tctccaagtg cgcttcgttg tatcttcttc attcggcttc
     4861 caccacttcc gaattcgcta gccaagaatg atacaagtac tctcttgttc aacaaatctt
     4921 agtcttatta aactttcttg aattatatca gaaaaaaaaa cgaaaggcta gtcgtttcat
     4981 aaaaaaacaa tccagatggc ctaaaggaga agaaatacca cctggatagc tataaatcat
     5041 ttcgtaaata cgaaagactt aacaaattaa tcagctacga taatcgttcc ttcttcagaa
     5101 gctaaaagat tctcgatatt ttctaaagaa gtgatgattg ccttagcgtt tggttttgct
     5161 tcaacaaatg caatagctgc ttctactttc ggaagcatgc ttcctggagc gaattgcttt
     5221 tcatcaatgt attgtttcat ttctgaaaca gtaacagttt ctaatttctt ttgatctggt
     5281 ttttggtaat tgacatacac attgtctact cctgtcaata cgatcagcaa atccgcatcg
     5341 atgatttcgg ctaatttttc tgaagcaaaa tctttatcga ttacggcttc tcgtccttct
     5401 aatccagtag ctgtttctac aacaggaatc ccaccgccac caacagagac tgtgataact
     5461 ccttgatcga ccagcgtttc gatgacacgt gcttctttaa tcgagatggg tttaggggaa
     5521 gcgactactt ttctccatcc acgtccagca tcttctttga atgtcacagt ggagtcagca
     5581 ttcatttgtt ctttggcttc ttcttccgtg tagaatggac cgactggttt acttggattt
     5641 ttgaatgaag gatcattttc atccacaata acttgagtga ctaaagaaac cacatcttta
     5701 tcgatccctt tttccttcag tacttcgccc atggcgtttt gtaaccagta accgatagat
     5761 ccttctgtca ttgcaacaca tgtatctaaa ggcattgccg gtgttttctc agaatcagca
     5821 gcttgctgct gaatcaatag gttaccgact tgcggaccat ttccgtggga gatgatcaat
     5881 tcatctcctt gttcgataaa tttcactaaa tactttgctg tttccattaa cgcttcttgt
     5941 tgggcttttg cacttgcatc cgtagacaag atggcattgc cccctaaagc gacaactact
     6001 tttcgtttgg ccatttcatt cgtcctttct tcagttctac cgaattttct agataacaaa
     6061 acgagccagc gttttgtgat tttccctcaa tgctgactcg ttttttcgtg agatgaatgt
     6121 ggttatgctt aaacgcgagg gataaacaag ttacctaatg ttgctgccat gatcgctttg
     6181 attgagtgca tacgattttc tgcttggtca aattgacgag catatttgct gcggaagact
     6241 tcatctgtta cttccatttc agtgatgcca aagcgttctt tcatttgttc accgtaaaca
     6301 gtgtttgtat catggaaagc tggcaagcag tgcaagaaga tcaaacgatc tgtgttgtgt
     6361 gttttttcta ccatttccat attgatttgg taaggtttca acaatttgat acgttcttcg
     6421 aatttatctt cttcacccat tgatacccat acatcagaat aaaggacgtc tgctccgtct
     6481 acacctttat caacatcgtc ggtaatcatc aattgtgcac cggatttttc agcaaagcct
     6541 tctgccattt tgacgatttc ttcttcaggt tgtaattctt ttggtgcaac gatacgcata
     6601 ttcgctccta aaattgcacc tgtgactaat aaagagttag ccatattgtt tcgaccatct
     6661 ccgcagtaag caacagtgat accttctact gttccaaagt tttcttggat agttaaaaag
     6721 tcagcgatca tttgtgttgg atgccattca tcagtcaagc cattccaaac tggaacacca
     6781 gagaattctg ctagttcctc taccattttt tgactgaatc cacggaattc gattccgtca
     6841 aacatacgtc ctagtacttt agctgtatct tctgtggatt cttttttacc taattggata
     6901 tcatttgcac ctagatattc aggatgtgca cctagatcga tcgctgcagt agtaaatgca
     6961 gaacgagtac gtgtagatgt tttttcaaat aaaagcgcaa tattttttcc ttctagatag
     7021 tgatgtggga taccacgttt tttcaagtct ttcaagtgtt ctgaaaaatc gatcaaatat
     7081 tgtaattctt cttttgtaaa gtctttttct gctaataagc ttcttccttg gaatactgat
     7141 tctttcatat taaacattct cctttagatt gtttttattt aagattaaag aggaaatccc
     7201 gacggcagct gacagtaaga ctgccgtcag gaaacaaatt acttcaagtc ttcacggaca
     7261 agtggttggc tcatgcagcg tggaccacca cgacctcgtg acaattcact tgaattgatt
     7321 tctaacactt tgatgccata gctacgaagc aattcgttcg atacataatt tcggttataa
     7381 gtgactacaa cacctggagc gattgccaat gtgtttgatc catcattcca ttgttctcga
     7441 ggtgcaacga tctcatctcc attaccagtt gggatcagta caagatcatc taatccaaga
     7501 gcagctttca tcgtttcgtg taagttatct gaatgagtaa tcttgatatc atcaccatct
     7561 ggttcgattg taaagacatc catcttaccg tctttacttt gaattgctgg atggatagtg
     7621 aatttatcgt gatctaccat tgtaaatacg gtatccaaat gcatcattgc acggttgttc
     7681 ggaattttga ttgccaacac tttttcaaat cctgaatttt ttgcgaatag gtggcgtgct
     7741 aatttttcta aagctttcgc attagttcgt tgagaaatcc caacagctac tactttatcg
     7801 cttaagatca attcgtctcc gccttcgata tgatctggat tttcacgatc caaccatact
     7861 tcgattcctt tatttgcaaa tcttggatga tgtttcaaaa tatattctgt aaacatcgat
     7921 tcacgacgac gtgcttcaaa agtcatctta ttgactgtca tcccatttcc cattgatgcg
     7981 gaagggtcgc gtgtaaagta caagtttggc attgggtcca tgtaaaatgg atagtcatcg
     8041 tctgctgaca gatcatatag actgctagaa cgtacttcga tatcttttgt acgaacccca
     8101 gccatgattt tatctaccat ttcttgcgtt tccattgata gaaggaactc atgaagaccc
     8161 tctcttacag cattcgatgc aatatgagat tcatccaaca ttttattcaa gaattgttct
     8221 ttgacattgc ctgcgtcaat cgcttctgcc gctaattttt ccaagtaaag agtctcgacg
     8281 ccttcatttt gcaatgtctt cgcaaagttg tcatgttctt cttgagcaat cggaagatat
     8341 ggaatatcat cgaacaacag acgatccata atatctggtg tcaaattttc cacttcttgt
     8401 ccaggccgtt tcagtaaaac tgtctttaat ttccctattt cagagaaaac gtgaataggt
     8461 ttatccatgt tgtgttgcct ccttcttttt gattacatgt ataggatatc ctccttttgt
     8521 aatcactttc ataattttgt ctcattcaaa atgagacatt cctcacatta atattcgata
     8581 attaaataat taaacgtttt tctattttta agcgtaattt attcattttt tattctgtat
     8641 acactttcaa aaaaatattt attcattgaa aaaatttttt tgaataaatt aaaaaaataa
     8701 attatattat cg
//
LOCUS       DACDMM010000111         8700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDMM010000111 DACDMM010000000
VERSION     DACDMM010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.112"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..995
                     /locus_tag="IWD16_002044"
     CDS             <1..995
                     /locus_tag="IWD16_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9089048.1"
                     /translation="GVSIATVSRVLNEDPTLSVTDETKRKIAESVNFYHYVKKSPVAK
                     NLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLSETPLEQDDLLK
                     CQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKATNQHLDRLYKK
                     GHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQIYLEGWTTLDG
                     MQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDISIVSFDNIEVAE
                     FLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVRESEKNID"
     gene            1289..2398
                     /locus_tag="IWD16_002045"
     CDS             1289..2398
                     /locus_tag="IWD16_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9089049.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            2481..3377
                     /locus_tag="IWD16_002046"
                     /pseudo
     CDS             2481..3377
                     /locus_tag="IWD16_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            3393..4217
                     /locus_tag="IWD16_002047"
     CDS             3393..4217
                     /locus_tag="IWD16_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9089050.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            4249..5559
                     /locus_tag="IWD16_002048"
     CDS             4249..5559
                     /locus_tag="IWD16_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP9089051.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            5707..6966
                     /locus_tag="IWD16_002049"
     CDS             5707..6966
                     /locus_tag="IWD16_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9089052.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            6976..7848
                     /locus_tag="IWD16_002050"
     CDS             6976..7848
                     /locus_tag="IWD16_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9089053.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            7860..8690
                     /locus_tag="IWD16_002051"
     CDS             7860..8690
                     /locus_tag="IWD16_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9089054.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
BASE COUNT         2911 a         1289 c         1736 g         2764 t
ORIGIN      
        1 cgggtgtgtc cattgcaaca gtatcgcgcg tgctaaatga ggatccgaca ctttcagtaa
       61 ccgatgaaac caaacgaaag attgcagaat cggttaattt ttatcattat gtcaaaaaaa
      121 gtcccgttgc aaaaaattta acaaatattg cattaataac gacagtttct gaagtagatg
      181 agttggaaga tccctatttt cgaacgattc gcagagggat tcaagtcgaa gcagaacaaa
      241 gcaaagtcaa tttgaagaaa attattcgtt tgtcagaaac accgttggag caagacgatt
      301 tgctaaaatg tcaaggtgtg ttgattattg ggcaggtgct atcttctgtg attgaagaag
      361 tatcagcaat taattcgaat gtcgtagtga ttgatgaccc tagtattgaa gctgaagtag
      421 atgcagttta tactgattta acaaaagcaa cgaatcagca tttagatcga ttgtataaaa
      481 aaggacaccg aaacattgcg tttatcggag gaggacgtgt tcgtttagat agccaaggaa
      541 ataaacacgt gtcagatgat gattatcgaa aaatagccta tgagacgtgg atgaaacaaa
      601 aaggattaga aaaatatagt caaatttatt tagaaggctg gaccacactt gacggaatgc
      661 aggcagcaga tttacttttg aaaaaacatg ctaaagataa gttgccgact gctatcatgg
      721 taggaaatga tcctattgct gtgggggttt atcgttcact tcaaaaaaat ggtctacata
      781 ttccagaaga tatttcgatt gtgagttttg ataatattga agttgctgaa tttttaacac
      841 ctgcattaaa tacggtgaat gtgaatacag aagaaattgg aaaactagca gttcgcatgg
      901 ttaatgaacg aataaaagaa gttagaaatg tttcgatcaa agtgattgtt tctaataata
      961 ttattgttag agaaagtgaa aaaaatatcg attaaaaaag taaacatcca aaaaagggtg
     1021 tttacttttt tgtttactaa tatttttagt aaaaaatctc gacaaaacat taagtaaaca
     1081 gttatggtgt tgttaacaaa aagaaaacgc attcaaaaca ggaggaatta agatgaaaca
     1141 tttcaaaaaa atagctttaa cagggatagc tgttttaact gggacattac tcgcagcatg
     1201 tggtggggga gatagtaatt ctaaagacgg gcaagtaact ttaagctatg cgagttgaga
     1261 tactgaccaa gctccaggac taagaaaaat gttagatgaa tttgaaaaac aaaatcctga
     1321 tatcaaagta gaaatggaga caacgccttg ggatcaatat tgggttaaat tagaagctgc
     1381 aacaacggga ggtaatatgc cagatgtagt aaccatgcac tctagcgaat cctataaata
     1441 tatgtcacag aatgctttga tgggacttga tgatgttatc aaagaaaatg atattgacat
     1501 gggaaacttt actgaaggaa ttgctgattt ctatacgctt gaagacgaac tatacgcaat
     1561 ccctaaagat gcttctgtag tggggctatg gtacaacaaa gaattatttg ataatgcagg
     1621 tgtcgattat ccagatgaaa catggacatg ggaaacactc agacaagctg caattgattt
     1681 aacagatgaa tcaaaaggca tctatggttt tgcatcacat attggaacag agactggcta
     1741 ctggccattt atttatcaaa atgggggaga agtatttgcc gatgacaata caaaatcagg
     1801 tttgaatacc ccagaagcac aagaagcttt agctttttat cgagatattg cacttgttga
     1861 tcatgcttca ccaacaatta cagaattaca agatcctgat aagttaagtc gttttcaatc
     1921 aggtagatta gcaatgatga ttgaaggaaa ctggtttgca ccgggatttt tgaaaaatga
     1981 ctatctagtt gaaaatgttg cttctgccgt gttacctaag ggaaaagtta atgcaacaat
     2041 tacaaatgga cttggttggg ctgccagtgc gagtacaaaa tacccagaag aagtgaaaaa
     2101 actaatggca tttttagcta gtgaggacgc caataaaatc caagcggaaa cgggggcatc
     2161 aatcccagca ctaaaaggct ttgaagacga gtgggctgct tcttcagata caattgattt
     2221 gagtgctttt ggaaaaatgt tggagtatgg agtccctcgt ccatacaata aagatggatt
     2281 acgagctgaa caagcagaag ttcaaattat taaccgtatt ctgtctggtg aagtaactgt
     2341 tgaagatgga accgctcaaa ttgaaaaaga agtcaatgcg atattgagcg gaaattaaat
     2401 tagttaatta tacaatatac aggcgaaagg attctatttc gtctgtatat taatggatga
     2461 ataccaaaaa gggggatatt atgaaaaatc gaatttttgg aaaatatcgt aaagatcaag
     2521 tgatatgggg ctatattttt atttttccaa cggcattagg attaatttta ttaaatatat
     2581 ggccagcaat tcaaacagca attttaagtt ttcaaaaaac ggtagggttc ggtgatactg
     2641 aatgggtcgg attaagaaat taccaaaaaa tgtttgcaga cggtgaagtt tttcagtctt
     2701 tattaaacac attaatttat gcagtggttt cgatacctag tattgttgta ttatccttgt
     2761 tggtggcagt tttaatgaat agaaaagtta aaggattgtc gatttatcgg acaatttatt
     2821 ttctaccagt cgttgctgca ccatccgcag tcgcaatgat ttggcgctgg atgttcaata
     2881 atgattatgg actgattaat aacatgttgg ctaaaatagg tctcgatgga ccagcatggt
     2941 taacggatcc atcgattgcg atttattcta ttatcattgt aggagtttgg agtgctattg
     3001 gttataacat ggtgttatta ctagccggct tataagaaat tcctaaagat tattatgaag
     3061 cagcggaaat tgatggtgct agtccaatcc agcagttttt caacattacg ttaccattag
     3121 ttacaccaac attatttttt gtagttgtaa caacagttat caatgctttc caagtatttg
     3181 atgttatttt tatgatgatt actcctaata gtaccgccat gtttaaatca caatcattag
     3241 cctatttatt ctataaacat tcatttattt taaatgacaa gggatatggt tcggcaatcg
     3301 ttatgttcct tttagtgatt atattaatta ttactgcaat tcaaatgaag ttgcagaaaa
     3361 agtgggtaaa ctattaagga ggctatcaaa ttatgaaaaa taaatttttt tcaaatgcgc
     3421 caatccatat catgcttatt ttaggtggta ttgcaatggc attacctttt atttggatga
     3481 ttttaacttc agggaaaacg ttggttgaat ctacccaaat tccacctaca attttccctg
     3541 agaaatttca gctaagtaac tatcaacaag tacttgattt attaccattc atggcttttt
     3601 atatcaatac atttttaatg gttgttggac gagtgattgg ctcggtttta ttttcagcaa
     3661 tggctgcata tgcttgtgcg cgtctaagtt ttccagggaa aaatatcctt ttcagcttag
     3721 tacttattca aatgatgatt cccggttcca tttttattac tccacagtat ttattagcac
     3781 aaaaattggg tattttaaat acaattccag cattaatatt accaggggta gttagtgcgt
     3841 ttggtacgtt tttattaaga caatttttct taggtttacc tgatgaattg gaggaagcgg
     3901 cctatttaga aggtgccaat atttggacga tctttttcaa aattatgttg ccgcttgcta
     3961 aatcaggatt ggttgcactt ggaatattca cagcgttgtt tgcatttaaa gatttaatgt
     4021 ggccattgat tgtcaatact tcaattgata agatgccttt agctccggga ttagcaaact
     4081 tacaaggaca atatgccacg aacttcccac aattaatggc aggttcgtta ttagcaattt
     4141 ggccaatagt tgtgatattc atcgttttcc aaagaaaatt cattgaagga attgctacaa
     4201 caggtagtaa attataaaac tattaattag tgattggaga gtaagtacat gacaaaaatt
     4261 acatttattg gtgcaggaag tactattttt gcaaaaaacg tattaggcga tgcgatgtta
     4321 actccaagtc tacaagatgc acatattgca ttgtacgata ttgatgaaaa acgactaaaa
     4381 gaatcaaatt taatgcttaa aaatattaat aaaaattcga atgaaaatcg tgctgatatt
     4441 gtttcttatt atgatcgcaa agaagcgtta agagaggctg attttgttat caatgctatt
     4501 caagtgggtg gttataaacc ttcaacagtg attgattttg atattcctaa aaaatatggg
     4561 ttgcgacaaa ctattggtga caccatgggg attggtggga tttttagaac cctacggaca
     4621 ctaccagtaa tgttagactt tgctaaagaa atggaagaag tttgtccaaa tgcttggtta
     4681 ttgaactata caaatccgat ggcgatgtta accatgggtg ttttaaaagc gacgagtatt
     4741 aagactgttg gtttatgtca tagcgttcaa gtatgcgtac cagaattgtt tgagcattta
     4801 ggaatagccg atcaatatga cttaaatgac tttcaatgga aaattgcagg cattaatcat
     4861 atggcattct tgttggaaat tacaaaaaat ggggaagatt tctatccaac aattcgtcag
     4921 ttagctgcag aaaaaccaaa tccacatcca gattctgtac gttttgaatt gttgaaacgt
     4981 tttggttact atattacaga atcaagtgaa cacaatgcag aataccatcc atactttatt
     5041 aaatcaaaat acccagaatt gattgacgaa ttagggattc cgttggatga atacttacgt
     5101 cgttgtgaac aacaaattaa tgattgggaa agcatgcgag aggacattgt aaataatggg
     5161 gagttaacac atacgagaag ccgtgaatat gcttcatata tcatggattc tattacgaca
     5221 ggaactccgt cagtgattgc aggaaatgtc ttaaataaag gcttaatcac aaacttaccg
     5281 acagatgttt gtgtggaagt accttgttta gtcgataaaa atggcgtgca accaacctat
     5341 gtcggtgaat tgccaacaca attagctgct ttaaatcgga cgaatatcaa tgttcaagaa
     5401 ctgacagtag aagccgcttt aacactagat aaatcaaaaa tttatcaagc agcgtattta
     5461 gatccacatc tatcatctga attgtcttta acagatatca aaaatttagt ggatgattta
     5521 atcgaagcac atggggacta tttacctgaa tataattaaa aagaagctga aagagtaagg
     5581 aattttcaat tccttactct tttcttagtt aaatttagta aaaatattga caaaacactt
     5641 agtaaacagt tacattagtg ttaacaaaaa gaaaacgcat tcaaattgta taaggaggag
     5701 cataaaatga agtggtctaa aaaaattatt ttagcttgta tagcactagg agcaacgatc
     5761 tctttgactg cttgtggaaa aaatcaggaa gatgcaagcg gtaaggtaga aatagagttt
     5821 tttaatcaaa aaaaagaaat gactcaaacg attcaggaga ttgctaaaga ttttgaagca
     5881 aaaaaccctg atattcatgt aaaagttgtg gatgtaccga atgctggtga agtaattaaa
     5941 acacgtatgc ttgcaggcga tgtaccagat gtcattaatc tttatcctca aagtatagaa
     6001 ttgaaagaat gggcgaaagc tggttattta gaggacttaa ccgaagaacc gtatttagag
     6061 aatatcaaaa atggatatgc acaacgtttt gctattgagg atcgtgtata tagcatacca
     6121 ttaaccgcta atgtctatgg tttttactat aacaaaactg cttttgagga aatggggatt
     6181 caagcaccag aaacttgggc agaatttgaa aaaatagtag ctgagattaa agatcaaaac
     6241 aaagtaccgt ttgcgattgc aggagcagaa ggctggactt taaatggtta tcaccaacta
     6301 gctatagcga ctgctgctgg tggtgaagag gaagcgaaca atgtttggcg tttctcagaa
     6361 gttaatggaa ttaatgctga aagcaaagaa atgcaaaaag attttaatcg attagatttg
     6421 ttaagagaac caaaagcttt gcaaaacaac tggcaaggtg ccggttataa cgatacagta
     6481 gttactttta caaaaggtga tgcgttgatt atgccgaatg gttcatgggc aatgccgatg
     6541 attcattcac aaaatccaga ttttgaagta ggaacttttc cttttccagc agatgaagca
     6601 ggtcaaagtc taacgattgg cgcaggagac ctagccttat cagtttctgc aacttctcaa
     6661 cataaagaag aagccaaacg ttttgtagaa tatatgacaa cacctgaagc gatgcagaaa
     6721 tattatgatg tagatggtgc accgtgtgct gttgagggcg ttatcgagaa tactcaggat
     6781 tcaccattaa gtggtttaac tgaacttgct tttactgatc gtcatttagt ttggttagcc
     6841 aaagattgga atagtgaaaa tgatttttat accttaacaa caaattattt acacaatggc
     6901 aatcaaaaag ccatgattaa cgcattaaat gctttcttta atccaatgaa agctgacgga
     6961 aggtaggaaa ataaaatgaa aataaaagaa attttaaatc gttactgggc aatcattttt
     7021 gtagcaattc caattatttt gcagatgatt tttttctact ttccaatggt tcaaggtgct
     7081 ttttacagtt taactaattg gactggtctg acttataatt ttgattttgt gggtttgaac
     7141 aattataaga ttcttttttc tgatccgaag tttatcaaat cgattggctt cactttaatt
     7201 gtgacgcttt gcatgattgc tggacaaatt ggttttggta ttttgattgc gcgggcgttg
     7261 aacgcaaata ttaaagggaa aacattcttt agagcatggt tcttttttcc agcggtctta
     7321 tcgggcgtgg ttgtttcttt agtatttaaa caaattttta actatggttt accaaaaatt
     7381 ggtagtatgt taggcattga atttttacaa accagtcttt taggaacaac atggggagcg
     7441 atttttgcag taatttttgt tttactttgg caaggaattg ccatgccagt catcattttc
     7501 ttagcgggtt ttcaaagtat tccacaagaa attattgaag ctgctagaat ggatggggcg
     7561 aatgataagc aaattttttg gaatattgaa atcacctact tattaccaag tatttcaatg
     7621 gtatttatta tggcattaaa aggtggctta acagcatttg atcaaatttt tgctttaact
     7681 ggtggtggac caagcgatgc cacaacatct ttggggttac ttgtttataa ttatgctttt
     7741 aaaaacaatc agtttggtta tgcgaatgct attgctttag tcttatttat cttaattggg
     7801 attgtttcac tggcacaatt aaaattatcg aaaaaatttg aaatctagga ggtttcccaa
     7861 tgaaaacatc aaaagaaaaa aatatttcta aatacttgct tttattatta gggggcttca
     7921 tgattttagt tcccttagtt tcaacgctat tcggttcatt taaaacaaca cccgatatta
     7981 tgaataattt ctttcgtttt cctaatccag cgactttgga aaatttccga agattgttag
     8041 cggatggaat tggtcattac ttttggaatt cggcaatcat cacaatcatt gcagtagttg
     8101 ctgtgacagc aattattcct atggctgctt attcactagc aagaaaaatg actcgtggga
     8161 aagcatacgg attgatgtat ttatttttaa ttttaggaat ttttgttcct ttccaagtaa
     8221 ttatgattcc gattaccgtt atgatgagta aaattggttt agcaaatatg tggggattaa
     8281 tcttactcta tctcacttat gccgtaccac agacactatt tttatatgtt ggttttatta
     8341 aactaaatgt gcctatcagt ctggatgagg ctgctgaaat tgatggggct agtcacttta
     8401 caacttattt caaaattgtt ttcccaatgt taaaaccaat gcatgcgacg actattattc
     8461 ttaatgctct atggttttgg aatgatttca tgctaccatt attaatttta aatcgtgatt
     8521 ctaatatgtg gacactacca ttgtttcaat ataattatac gggacaatat tttaacgact
     8581 atggtccaag ttttgcttcc tatacagtag gaattgtcac gattacacta gtttatcttg
     8641 ctttccaaag aagtattata gctggaatga gcaatggagc agtgaaataa ccaataaatt
//
LOCUS       DACDMM010000112         8584 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDMM010000112 DACDMM010000000
VERSION     DACDMM010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.113"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(190..2571)
                     /locus_tag="IWD16_002052"
     CDS             complement(190..2571)
                     /locus_tag="IWD16_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9089055.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2561..3208)
                     /gene="recU"
                     /locus_tag="IWD16_002053"
     CDS             complement(2561..3208)
                     /gene="recU"
                     /locus_tag="IWD16_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP9089056.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3272..3811
                     /locus_tag="IWD16_002054"
     CDS             3272..3811
                     /locus_tag="IWD16_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP9089057.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3901..4320
                     /gene="gpsB"
                     /locus_tag="IWD16_002055"
     CDS             3901..4320
                     /gene="gpsB"
                     /locus_tag="IWD16_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP9089058.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4342..4709
                     /gene="rnpB"
                     /locus_tag="IWD16_002056"
     ncRNA           4342..4709
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWD16_002056"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4918..6084
                     /locus_tag="IWD16_002057"
     CDS             4918..6084
                     /locus_tag="IWD16_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP9089059.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6130..7626
                     /locus_tag="IWD16_002058"
     CDS             6130..7626
                     /locus_tag="IWD16_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP9089060.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7707..8390
                     /locus_tag="IWD16_002059"
     CDS             7707..8390
                     /locus_tag="IWD16_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9089061.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2692 a         1563 c         1827 g         2502 t
ORIGIN      
        1 aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa atgttagatc
       61 acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg gtacatgatt
      121 tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt agacgtggtt
      181 tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat ggtcatttgc
      241 agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag atgaagacgg
      301 tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg gctcgcttgt
      361 ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac tagtagagct
      421 agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat agctggaaga
      481 tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat ctttgtagta
      541 cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat ttgtcacgtt
      601 agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg acgattctga
      661 agtgacaggt gtcatttttt tattgtaacc tgtccacacg gaaatcgaat aatttggcgt
      721 atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt ttgcatactc
      781 gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag cgatttgcgc
      841 attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca tataagctgt
      901 ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc cgtcttggaa
      961 gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga aagccgcata
     1021 ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt cttccgtatt
     1081 actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat tttttaagaa
     1141 agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa cattacggga
     1201 taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc agtttcctac
     1261 aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc cagtagaata
     1321 tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag aaccaaaatc
     1381 acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga tgtgacgtcc
     1441 gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg caacttgcat
     1501 ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata gtttcttttg
     1561 ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat tcttatctgt
     1621 tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct tagtattgtc
     1681 atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag cttgatcata
     1741 ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat ctcgtctttt
     1801 cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag gcatcccagc
     1861 aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt acatctcaga
     1921 agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt agtaggtcaa
     1981 gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt cttgggcctt
     2041 gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga tcaattgctg
     2101 tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg cagaaccgat
     2161 gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta ccgacacgat
     2221 cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct tttctgcgcc
     2281 taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg tagcgtctaa
     2341 ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc ctacacctgc
     2401 taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa ataatccttt
     2461 tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca cactcttttt
     2521 aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca ttcttgcttt
     2581 ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa ggaatccgcg
     2641 gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa ggtagtgatt
     2701 tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta ttcaaataaa
     2761 aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt tgttcgaggc
     2821 atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa gatgttttgt
     2881 tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca ttatagtctg
     2941 tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc ttaggatagt
     3001 ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac ccgcgggaaa
     3061 gataatattt attactttca ttgattgctt cttcaaagcg catcccccga ttgccgaatt
     3121 cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc ttataataag
     3181 gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca cattatagca
     3241 aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa tctatgtgtc
     3301 tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa tccaagttgt
     3361 taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg aatgggtact
     3421 tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg aattgaaaga
     3481 agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg gtaataactg
     3541 gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt atgtcgaagc
     3601 tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc gttttatact
     3661 tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa aaacgaagta
     3721 tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc ggctgatcag
     3781 tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt atcaactttt
     3841 ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag aggtgtaaat
     3901 atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa aacaaaaatg
     3961 cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa agattatgaa
     4021 gcttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag tgcaaaagta
     4081 gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac agaggcacca
     4141 aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct tgaacgtgaa
     4201 gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca acctaatcca
     4261 aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg tcaattttaa
     4321 tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat caggctgagg
     4381 aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg aaaccataag
     4441 ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct atgcttaagt
     4501 atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg gaacgcggta
     4561 aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg aagaagaaca
     4621 ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt ctccctggaa
     4681 aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt aggtcgtgac
     4741 aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc aatacttcag
     4801 atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga atttgagtaa
     4861 aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag attgtgtatg
     4921 gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga agcactggtt
     4981 ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg tgcacgtttt
     5041 gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc agatcgagta
     5101 aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga aaacgtaaaa
     5161 gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc tggaaaatcg
     5221 atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa agcaatcgtt
     5281 gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga aactggcgca
     5341 cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct ggatacgaca
     5401 ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc attgaaagaa
     5461 aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc attttatgat
     5521 cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca taatatcgca
     5581 ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca agaaatcatg
     5641 gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt agatattact
     5701 ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga agaaatcgga
     5761 ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac ggataaagaa
     5821 tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga agaaactgtt
     5881 cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg gagtaaatat
     5941 atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac caaaaaaaga
     6001 aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac tcgtccgcct
     6061 agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg atgtttggag
     6121 gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa tcttctacaa
     6181 caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa agctagtgca
     6241 taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa aatcggtcca
     6301 aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc ggaagttgga
     6361 actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt accggaagat
     6421 cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca aaaagctaga
     6481 gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga attaacaaaa
     6541 cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt gctgaatcaa
     6601 tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct taaagaagga
     6661 attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac agacttttta
     6721 aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat cgaacaactt
     6781 ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat gactggaatt
     6841 aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa aatggctgtt
     6901 tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga tgataaatat
     6961 gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca atcgttgttt
     7021 aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc tttctttcaa
     7081 gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg ttcattgaac
     7141 catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc tctccatatc
     7201 atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt tgctgaacta
     7261 cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac aaatgatgtg
     7321 gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt tccctcttat
     7381 gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga aatagatgtg
     7441 gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc agagcatatc
     7501 catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac acatgaaccg
     7561 cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt atatcaggta
     7621 aaataaaata caatttacaa agatagctga aattcatcta atctatagat gttttcagct
     7681 atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct gattatagta
     7741 tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa agagttgtca
     7801 agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg acgcttgcag
     7861 cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac gaggcaaagc
     7921 aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc tcgatgattt
     7981 tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc gaactatctc
     8041 tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca atcggcattt
     8101 tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct gaactgtatc
     8161 cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc aatcattttt
     8221 atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat gaagaagcca
     8281 aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa tggtctattg
     8341 acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga tggaggttgt
     8401 gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt acttctggaa
     8461 cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag gaacaatttc
     8521 aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat tggcaggaga
     8581 acac
//
LOCUS       DACDMM010000113         8494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDMM010000113 DACDMM010000000
VERSION     DACDMM010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.114"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IWD16_002060"
     CDS             complement(<1..173)
                     /locus_tag="IWD16_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9089062.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IWD16_002061"
     CDS             complement(593..1714)
                     /locus_tag="IWD16_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP9089063.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IWD16_002062"
     CDS             2042..3451
                     /locus_tag="IWD16_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP9089064.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IWD16_002063"
     CDS             3448..4176
                     /locus_tag="IWD16_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9089065.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IWD16_002064"
     CDS             complement(4304..5215)
                     /locus_tag="IWD16_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9089066.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IWD16_002065"
     CDS             complement(5232..6239)
                     /locus_tag="IWD16_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9089067.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IWD16_002066"
     CDS             complement(6236..8365)
                     /locus_tag="IWD16_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9089068.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IWD16_002067"
     CDS             complement(8370..8477)
                     /locus_tag="IWD16_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089069.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DACDMM010000114         8431 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDMM010000114 DACDMM010000000
VERSION     DACDMM010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.115"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            94..1248
                     /locus_tag="IWD16_002068"
     CDS             94..1248
                     /locus_tag="IWD16_002068"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP9089070.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="IWD16_002069"
     CDS             complement(1270..2022)
                     /locus_tag="IWD16_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9089071.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="IWD16_002070"
     CDS             complement(2386..5052)
                     /locus_tag="IWD16_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9089072.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="IWD16_002071"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="IWD16_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9089073.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="IWD16_002072"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="IWD16_002072"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP9089074.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2484 a         1736 c         1492 g         2719 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa
     8101 tcaaatttat ttgaaaaaac atttgcaata aatcagaaaa tagtggtcgt ttatttgtat
     8161 tcgctattct aataataaat aaccactatt tgatttatat atatttcagt atccttcttg
     8221 ttttcatata accaaaggcg ctatgatttc catatgaaaa aaataaagtc aggtgaaaaa
     8281 aatgaaactt gaggataaaa aagtcatttt gaagaaaatc aaacgactac cagaccaaat
     8341 agatggctat acgatctgtt ctttcaaaaa agacaggagt ataaaagctt atacgcaaaa
     8401 cacagaaaaa ggaataatct atttcctaca t
//
LOCUS       DACDMM010000115         8409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDMM010000115 DACDMM010000000
VERSION     DACDMM010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.116"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..279
                     /locus_tag="IWD16_002073"
     CDS             <1..279
                     /locus_tag="IWD16_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HAP9089075.1"
                     /translation="MSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAY
                     IINTENITSISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            455..1198
                     /locus_tag="IWD16_002074"
     CDS             455..1198
                     /locus_tag="IWD16_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9089076.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            1191..2771
                     /locus_tag="IWD16_002075"
     CDS             1191..2771
                     /locus_tag="IWD16_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP9089077.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(3022..3501)
                     /locus_tag="IWD16_002076"
     CDS             complement(3022..3501)
                     /locus_tag="IWD16_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP9089078.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3517..4269)
                     /locus_tag="IWD16_002077"
     CDS             complement(3517..4269)
                     /locus_tag="IWD16_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP9089079.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4386..4456
                     /locus_tag="IWD16_002078"
     tRNA            4386..4456
                     /locus_tag="IWD16_002078"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4418..4420,aa:Gly,seq:ccc)
     gene            4817..5542
                     /locus_tag="IWD16_002079"
     CDS             4817..5542
                     /locus_tag="IWD16_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP9089080.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            5797..6063
                     /locus_tag="IWD16_002080"
     CDS             5797..6063
                     /locus_tag="IWD16_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP9089081.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            6060..6491
                     /gene="ruvX"
                     /locus_tag="IWD16_002081"
     CDS             6060..6491
                     /gene="ruvX"
                     /locus_tag="IWD16_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP9089082.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            6516..6821
                     /locus_tag="IWD16_002082"
     CDS             6516..6821
                     /locus_tag="IWD16_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP9089083.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(6902..8047)
                     /locus_tag="IWD16_002083"
     CDS             complement(6902..8047)
                     /locus_tag="IWD16_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9089084.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(8108..>8409)
                     /locus_tag="IWD16_002084"
     CDS             complement(8108..>8409)
                     /locus_tag="IWD16_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9089085.1"
                     /translation="NLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
BASE COUNT         2689 a         1434 c         1637 g         2649 t
ORIGIN      
        1 atgagcatgc caggttataa aaataaaata ggcctagtat tgaaagatga agagattctc
       61 gtaaacggca caattaaaaa atataaaaaa tcactcgaat atgagtattt atgtaaagat
      121 ttaaaagctt atatcattaa tactgaaaac attacatcta ttagcagagt aaacgaaata
      181 gtaacatttg caaatggaga acaactagag atgggaaaga gaagtattga taaactaaag
      241 aattttatgc aagaaaagat agaaaatgaa tcattttaaa gaaaacaaag actcatatct
      301 tacctgttag aagagtgttt tcctgtagaa gagaacaacc caagtagcag cctagtcgtt
      361 tctatccttt tttgttatgc actctttttg acatttactc caccaataac gaagggtaca
      421 ataaaaagta ataacaaaca aaaggagtac agaaatgatt gaattcatca atgtaagtaa
      481 aagttacggg aaaaaacaag cattaaaaga cttgtctctc tcgataagac aaggggaaat
      541 ttttggattt ttaggacata atggtgccgg aaaatcaact acgattaaaa gtttagttag
      601 tattattgaa ccatcatcgg gaacgatttt agtagatgga atgaaattga cggaaaatcg
      661 attgagtatc aaacaaaaaa tcggttatgt gcctgattcc ccagatattt ttttacaatt
      721 gacggctgga gaatattggg acttgatttc agctgcctat gaattagaca cacaaaaaaa
      781 agaaaagcgt ttagcagaat tgacagcgct ttttgatatg tatagtcatc aaaatgaaac
      841 aatcgcaagt ttttcacatg gaatgcgtca aaaaacgatt ttgatcggca cgttgctgcc
      901 agatccagat atttgggtat tggatgaacc tttgcaagga cttgatcctc aagcagcatt
      961 tgatttgaaa gaaatgatga aggctcatgc cgcaaaagga aaaacagtca ttttttctac
     1021 acatgtattg gataccgcac aacagttatg cgataaactg gctattttga agaaaggtga
     1081 attgatttat cagggggcag ttagtgaatt attgaatggc tcacctgatg aaacgctaga
     1141 aaagatttat ttgaaaatgg ctggaaggca agcgacagga gatggaccgt atgtataagc
     1201 gacaattatt agaattaatt cgagttaatc ttttatatgt taatccacag ctcacgaata
     1261 aagcaagaga gaaaggaaaa aagggaaagc gattaactat ctatcttttg tctcaaaatc
     1321 ttttttcagg gctgattttt ttactgattt acggatttgc tatgtttgct atgaactttg
     1381 ttaaattgcc aggtttcttt acttattacg tggcactttt tggtatgtta ggtttaactc
     1441 aaagtatatc agtcatttat aatatctttt ttgaaggaaa tgacctgcaa gtatttttgc
     1501 ctctgccttt tggacaagaa caggtttttt cagcaaagat tttggtagtt gctttgacca
     1561 tcattccttt tgtttttccg cttctagtca tgtttatttt aaccggatgg cgagctgatc
     1621 tagggatttt gcttactatc attatctcaa tccttttgtt ttctctattt ttgattattc
     1681 ttttttgtct ggctagtttg attgtttttg gtttggctcg aacagcagtg tttaaaaaac
     1741 atagaaaaac agtttctagt atcttgcttg gcagctcgat gctgatcgca tttgctggaa
     1801 tcatgtggat gaatcatcaa acggggacta ttgaaaatga gttaacagat cggcatccga
     1861 tttcgatatt attgccattc tatgatgctg ccagtcagcc actttctcaa caaggtttat
     1921 tgggttgggg aatactagct atcacggctc tgggattttt ccttgctgta aaacagctga
     1981 tagtaccaaa gcttacagag caactgactg attcaaacag tgagttcaaa ataaaaagaa
     2041 cccataaaaa gaaccaaaat gtgcatcagc ttttatttag ttacaatgct caattagtaa
     2101 aagaacctaa tttgattatg caagttcttt cttcttcctt gttaactcca atcattttta
     2161 ttcttgcttt tgccctagga ggcgaaataa aattaactga tttagataat aagttaatcg
     2221 gtgttgtttt cctggtaggg atcgcattgg ctttcttaac agttaatcag acttctttta
     2281 tcagcaatct tatttctttg gatcaagaaa attttctgtt tatccgttct ttgccaatct
     2341 ctatgaacca atatttaaaa gaaaagttca gattcggatg gatattgcag agttttttaa
     2401 ctggtgggat cgcattatta agcggactgt cttttcagct gcctttgttt tttatcccta
     2461 gcttgctatt tggttcttta ttcggctgct acttattgtc actacgttat ttcgctagag
     2521 attatcggtt gctgctgctg aactggacca atattaacca attatttaac agaggttcag
     2581 gaagtttggg tttagtcgca actatgatgg gttcattgat catcagtatt attcttatcg
     2641 tcttatacgg aatgttggct ttgttttatc cattttggtt agtgaatatt cctgtgatcg
     2701 gtttagttct aatactttcc gctctatgga tcgtttatta tcaacgaacg ttttggaaaa
     2761 aatttgaata aagaagaggg gccgtgacaa aaatcatgtc gcagcccctt ttttacagat
     2821 agagggtgtt caaaagtcag tttctcagta ttaagtcaat tttatgtaaa aatgtgagaa
     2881 gctattttta caaaactttt tttaatactc ataattactt cttattgctc ctgcggttac
     2941 ttgtcgcaat gtttgtgaga tattttgtca tcgctcaagg ctgaacgtct ctttcacaac
     3001 cttttctttg ttacacttcg tttatttaaa aagccgtttt tttcgtttca tcatgattgc
     3061 gtcacgcttt actttgaaat tacggatgtt atggttaaag acttctgata atacaagacc
     3121 taaagcgatt gctccagcaa tcataattgc ttggaccgca taatgagccg cttctcgata
     3181 ggattcagta acgagatttc gaaccgcttg ataagctaaa ccaccaggga ctagaggaac
     3241 catcccggga atattgaaaa tcgttacggg cattttcaaa cgtttagaga aaagatcgct
     3301 gatgaatgct actcctaaag aaccaagcaa tgaaccaaaa gcagcagatc cgcctatttg
     3361 aacacagatc cagtagatca tccagccaaa agcaccagaa agaccacaac aaatcaatga
     3421 acgcctaggt acatttgtaa taacggcaaa tgccgcagtc gctaaaaaac taaaagaaaa
     3481 ctgaataaat atcgtcccca tacttgtgtt cctttcctaa taaaaaagct ggaaaataaa
     3541 agcaatcgca aaaccgatca tcgtagcagt catcatcgct tctgttccgc gagaaactcc
     3601 tgagacataa tgtccagcta gcaaatcacg gatagcgttc gtaatttgaa ctccaggaac
     3661 taaaggcatc acacatccga taatgattag atcctgattg cttcctaagc caaaacgtac
     3721 gctgtagata gcggctatgc caatcaataa agaagataaa aattccgata aaaatttgat
     3781 tctaaaaaat ttcaaactat atagataagc tgcgtatccc aatcctccaa tgactaaagt
     3841 cacaggtaaa tcggtcatct ttccgccaaa taacaccata attgttccac tgatcactgc
     3901 cgcgctaacg atttgcaacc agacaggaaa gaaagaacgg tctcggtcga ttgcttgcag
     3961 ttcgcagtaa agttcttcta atgttagctc tgaagcaaca tatttacgtg aaagatcatt
     4021 tacttttact actttttcta aattaatcga acggttggta atttgttcca ttcggatggt
     4081 cgaggtacga tcaagaccaa taaagagacc ggtttgagtg acatagctga ccaaacggaa
     4141 atttccagaa gctaaagcaa ttcgactcat cgtatcttct acccgataca tctccgcatt
     4201 gctttccatc atgattttcc cagcgagtaa acaagtgtcc agcaacagat ctaattcttt
     4261 tcccgtcata gcatcttctc caataattga ttgattagtt ttattttact acaaaacata
     4321 ccagaagcgt aaagaataaa aaaacttgag gaaggaataa aagaggcgta tactgttttt
     4381 gtattgcggg tgtagtttag tggtaaaatc ccagcttccc aagctgatgt cgcgggttcg
     4441 attcccgtca cccgcttaaa ataggttgtg aaaaagctgc tctgcaacaa gtaataagaa
     4501 cagccaagca aaaacaactt cttatgtttt taacttggat ggacttatta ccgcagatgc
     4561 aagcttttgc gcaccgttta ccaagcggtt gtgaaatcag tgtattgtct tgagtatgaa
     4621 gaaaattttt gaataataat aaatacctta cagatagagt ggtgactgat tagagacgag
     4681 gaatccgtct cttttttgta tttttaagcc atcttaaaac aggttttatt ttttgctgtt
     4741 tctatttctt tctagtattt atttataggg gaacatatgt taagatttag ggagctgtta
     4801 gtaaaggagt aaagaaatga gcaaaaaaac aaccaaggcg atcgtgggga tcgtcgctgc
     4861 tgtgattgca ggggcattcg gtgtttccac cacacaaaaa gattcagata tctttcagca
     4921 gattacaact atttttgatt ccacaccaca agcttctcaa gtagcggatg tgccagaata
     4981 tgatggaaaa catcaagaaa tagaaatcaa tgggaatcag ccggaattta caaaagagga
     5041 tcttagcctt tccaaaggaa catgggaaag ctattcaaat attgataaat tgaacagagt
     5101 aggacaagcc aatgctatgc ttggaaaaga tttgttccct actgaaaaga gagagccttt
     5161 gtatattgac ccgacaggtt ggaaacaaaa gaaattaagt gacgggcaat ggttatataa
     5221 tcgcagccat ttaattggtt tccagctgac tggtcaaaat aacaacatta aaaacttgat
     5281 gacagggaca cgttcgttga atgctccata catgcttgct cacgaaaatg atattgcggc
     5341 atatatcaaa gaaacaaatc atcatgtacg ctaccgtgtc actcctcatt ttgaggggaa
     5401 tgaactggta gccaggggag tgcaattgga agcagaaagc attgaagaca aaaaaattga
     5461 attcaatgtc tttatctaca atgttcaaga tggctacacg atcaattatg agacaggaca
     5521 agcaacaaaa acaaaaaatt agtagcagct attcgaattt tttatgaata ttatgaaaaa
     5581 attgtgtaaa aacatacatt caaagaaata tagcgtatac tttatgaaaa gagtactttt
     5641 gataaagcta cagaaaaaag gaaaaaaaca tacaaatagt gtttcttaat ttattcggaa
     5701 gtttggaata tttgtttgta gttttccctt tcttcgagta aaataaggtt gtagaaggta
     5761 agctggaatt accttgcggg aaagaggtgt agaatcatgg gttttacaga tgaaaccgta
     5821 cgttttggtt ttgacgatag tcgcaaaaaa gaagtgagcg aaacattagc gattgtttat
     5881 cgggctttgg aagaaaaagg ctacaatcca atcaatcaga ttgtcggcta cttgctttct
     5941 ggagacccgg cctacattcc tcgttatcgt gatgctcgaa atttgattcg ccgtcacgaa
     6001 cgcgatgaga tcatggaagt aattgtcaag gactacctct caaatcatgg agtgaattta
     6061 tgagagtaat gggcttggat gtcggttcta agacagtagg tgttgctatc agtgacccct
     6121 ttggctggac agctcaagga gtagagatca ttcggatcaa tgaagcagaa gaagagtttg
     6181 gctttgatcg gttgaaggaa ttagtagcac aatatgaagt agaccggttt gtagttggat
     6241 tacctaaaaa tatgaacaat tccatcggac caagggcaga agcttccatg tcatacggag
     6301 aaaagatcaa agaactattc tccttgccag ttgattatca agatgaacga ttaaccactg
     6361 ttcaggcaga acgtatgcta gtagagcagg caaatacttc aagagccaaa cgaaaaaaag
     6421 tcatcgataa attagcagct gtgatgatct tacaaaatta tttagacggc tcaggtaagc
     6481 aattatttta ataataaaag aaaagaggta atcttatgac agaacataac cattcccacg
     6541 accatgaagg acacgaacac atcacattgg tggatgacca aggaaatgaa acattatatg
     6601 aaatcctact aacagttgac ggacaagaag aatttggacg taactatgtt cttttatatc
     6661 ctgctggtgt ttcagaagat gaagatgtag aattacaagc ttatgcatac atcgaaaacg
     6721 aagatggaac agaaggcgag ttagaacaaa tcgaaacaga cgcagaatgg gacatgattg
     6781 aagaagtatt caatacattc atggcggaag aagaagaata gactataaag ctaggagaaa
     6841 ccttgatatg atagggtttc tccttttttt attttttact gtatgccata ttgtatgcca
     6901 tctagtcaat atttaagtgt tgtactaatt tttcaacagt cttttctttc gtttcttttg
     6961 ccaagtgacc gtaacaatcg atcgtctgac taatttttgt atgccgtaat ctagtttgaa
     7021 cttctaccat agtagctcca gcgtccaaga gcattgaagc atgagtgtgt cttgctacat
     7081 gcacaccgag aaatggaacc atagcttttt cacaaatagt aatcaatctt gcataaatag
     7141 cgtggtgtgt gattagttct ttatagatac cacaaaaaat catagtcgga tcagttacac
     7201 caagtgtcag catatatttc cgctgattaa tctgccattt ttttaatata gaaattgttc
     7261 tttcatctag tggaagaacc ccagcactac aatcagtttt aggatcactg attttatagc
     7321 catttttgga ttggcttagt gttttattga tggtgaccgt attttcttga aaatcaatgt
     7381 ctgaccaatt caatgcaagg gcttcactga tcctaacccc gctataaaag agaaagcgta
     7441 gtaacgtttt atcaaagtcg tttcgataag tttcaggttt gctgtttagg taatcaaaca
     7501 aggtgatcag ttgttcttta ctgtaaactt tgaccttttc tttatgctta gtggtggttc
     7561 ttttaggcat taatacgttg tctagtggat tagtctcaat aatcccaaga ttgatagcgt
     7621 agctaaaaat acgcttagaa gttgaatgaa gtaccttgta ttggtcagta gtttcagccc
     7681 agctatttac aatttgttgg gcttttttta ccgtgacttc attcacacga agatttccat
     7741 attttggaag aatccacttt ctaaacttga tttcagtttt catgaaggtg gcttctcgga
     7801 cagtggtttt atatgtttct agccacaagt cataaacttc tttaaacgtt tcgttgttcg
     7861 cttttttaat accgttttta tcgtagtcca cttgcaagcg tgacaaggca agctgggctt
     7921 ctttttttgt tttgtaacct cgtctacgaa ccttatcagg tttgccagtc attttgttca
     7981 ttccaagata ggcttcatag agccaaaatt tacctttttt agtttcatat tgcttgaatg
     8041 ttgccataat agttttccct ccatagtgta gcaagccgtg ggggcggttg gaggaggcag
     8101 tttattatta ttttgaatag tgtttattta gctcagtaac aagagctgtg aactctttta
     8161 gaagatcatt ttcataaaat tcttgatact cttttttctc atctgtattt ttttctgaat
     8221 cattaattaa tgagtcatat cgtaaaagcg tatttagaag agctgcgaga taagcaattg
     8281 gggtactccc gtcaggaaat tcaattcgag atcctgtttt aagatatttt attgcgtttg
     8341 ttaaataaat cttctgtgag acagtcaggt tatcttcttc gatgttattg atagcattag
     8401 aaagattag
//
LOCUS       DACDMM010000116         8358 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDMM010000116 DACDMM010000000
VERSION     DACDMM010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.117"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..367
                     /locus_tag="IWD16_002085"
     CDS             <1..367
                     /locus_tag="IWD16_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089086.1"
                     /translation="LLAGNITTIWLCWQQSAPLFYLLSTLLSVITLFLVFAMLNWVVI
                     KSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLGIYFRILMILPVIFLFGGTAVTVIL
                     ISKLTERALAKLPCQIQSH"
     gene            397..2310
                     /locus_tag="IWD16_002086"
     CDS             397..2310
                     /locus_tag="IWD16_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP9089087.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            2438..5419
                     /locus_tag="IWD16_002087"
     CDS             2438..5419
                     /locus_tag="IWD16_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP9089088.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            5416..7077
                     /locus_tag="IWD16_002088"
     CDS             5416..7077
                     /locus_tag="IWD16_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP9089089.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            7117..8049
                     /locus_tag="IWD16_002089"
     CDS             7117..8049
                     /locus_tag="IWD16_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP9089090.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         2757 a         1375 c         1729 g         2497 t
ORIGIN      
        1 gttgttggcc ggcaatatta ctacgatctg gctttgttgg cagcaaagcg caccattgtt
       61 ttatttatta agtacattat tatcggtaat tacactgttc ctagtctttg caatgttgaa
      121 ttgggtcgtg atcaaaagtc ttgggttgtg gatgatggga gaccgactga ttgactattt
      181 gcttttcaca gcaacgatgt tgccaacggt agtcatgcaa ggcttggtgc ttggaattta
      241 tttccgaata ttgatgattt tgcctgtgat ttttctgttt ggcggaacag ctgtaacggt
      301 aatattgatt agtaaattaa ctgaaagagc gttagcaaag ttgccttgtc agatacaatc
      361 gcactaattt tttcacaaaa aggagtattt ctttctatga agttaactga taaacaagta
      421 gctaatatag cctttatcca gcaacttgag attccattga cacaaaaaga tgaagaacaa
      481 atagacttga tttttactga cacaaaggct aatgtgcgac aaatcaagat tactaaaaat
      541 gagcagcaac gaatttgctt gtcttattca catgaagact tgctgtatcg aggattgttt
      601 atctgccttc cacgtttttt agctcaagga ggagagatgc catacattga agaaacctcg
      661 atcattgaga ccagttattc attagatgct tctcgtaatg gcgtgatgaa taaagagact
      721 ctaaaaaaat tcatttctta cttagcgttg atggggtaca acacattgta tatttacaca
      781 gaagatacct atgaagtaga agaccttcca tattttggtt accttcgcgg agcgtataca
      841 aaagaagaag tgaatgaaat tgttgaattt gcttctggtt ttggcataga agtagttcca
      901 tcgatccaga cccttgctca cttgacccaa tttttgaagt ggtatgctca acaagaactg
      961 atggatgatc aaaatactct actgatcggt gaagaaaagg tttatgcggc aatcgaaaag
     1021 atgattattg cctgcaaaga aatgtatcag acagagcgta ttcatttggg aatggatgaa
     1081 gcctatcaag caggtctggg acgatattta agcctgtatg ggtattgtga tcggacaact
     1141 ttaatgattc aacatatgga ccgtgtgtta tcaattgttg ataaatataa gatgaaacca
     1201 atgatttgga gtgatttcat ttataagcta ttagatgaaa ccggtaaaga taggtattac
     1261 aatcctgatt ttaaactcaa agcattagca gctaaaggat taccagcagg attaacctat
     1321 gttcattggg actatggttc agatgaagtt tcctcctatg agaaagtaat tgataaccat
     1381 ttgaaatttt gtgacattga tcattacgtt ttagctagtg gtgcccatat ctgggggaga
     1441 ctagccccga atcatgggaa aacagaagca attgtttctg cagctgtaaa agcttctaaa
     1501 aataaaggtg tacgttctgt gatgttaact acttggggag atgaagggca ggaaacagag
     1561 cattggcatt ccttaattgg ggcattaaaa ttagcagatt atgtctattc tgaagaagag
     1621 tggaatgatt ttgaggaaat ctacgatcgt ttattaggaa caggtagtta tcaattaatg
     1681 tccgaattac gatatttcga tgaagtggat accattacaa ataacaactt acaaatggct
     1741 tccgtgagta aactgctact ttggcaagat ccgttagttg gtatatatga ctttcatgta
     1801 gccgctcata acaaaattgc cactagaaca attggtactt attatcatga gttagctcag
     1861 aaattagata tgaataaaaa ttggaatcca aaacaaccaa ttttaacaac tattcgtcaa
     1921 cgatatatac ttttagctga aattttagca caaaaggcag aactaggcat ctatttacaa
     1981 cactgctgtc tggtagaaga ccttgcagga ttaatggaat tagcgaatcg gtgtgaagca
     2041 ctagccaatc gagtaaatga attggcatct attcatgaaa taatttggta tcaaacatac
     2101 aaaacctatg gttgggaagt attagaacaa cgatatgctg cattagctca tcgtctgaaa
     2161 accgttgcgc gtcgtattaa aaatcatctt caggggcaag atgccctaga ggagcttttg
     2221 gaagaacgat taccgttttg tcaatatcaa gtgccgcttg aaatttctgg ctttaattat
     2281 cggcaaagct cggtgtcagg ctacaattaa cgaaggaatc ttcccgttga acggaccact
     2341 tgctcatcta atgggaaagt ggttcgtttt ctattcataa gtagcttacc actaactctt
     2401 ttattaagaa gagaaaaaaa gtaggaagtg agaaaatatg ttatcggact attatcaaaa
     2461 tctgtcagtt ataagtgtca atacagaacc attacgtaat tattatattc cttatgcaag
     2521 taaagaagaa gcaaaacaag agttagaaag ggaaaaaacg agccagttta tcgatctcaa
     2581 tggtacatgg gattttcagt attatccttc tgtccaacag ttaccggaaa atttttttca
     2641 agaggccttt actgaaagct atgatagaat ttctgttcct ggagtttggc aaacagaggg
     2701 atttgactta cctcagtata tcaatgtatc ttatccaatt ccttttgatc cgccttatgt
     2761 acctaatgat accccttgtg gtgtatatcg gcgtcagttt caaattgagc aaacagatgc
     2821 acgcttttac ttaaatctag aaggagcaga tagtaattgt tttgtttgga taaatagaaa
     2881 ctttgtaggc tatgaccaag tgagtcatag tacatctgaa tttgatgtga ccaagtattt
     2941 acaggttggg gttaatgaaa ttcaaattct agtgatgaag tggtgtgatg gtacctactt
     3001 tgaagatcag gataagtggc gaatgagcgg tctatttcgt ccagtgtatc ttcttcaacg
     3061 accaactatg catctgacaa gttatcaact acggacgttg actgatccta aaaaaggaag
     3121 cggaacatta atggtttctt ttcaacgatt tactcctatt gaagtatcgt tacctatttc
     3181 ttacgaatta ttccatcctg atggacgtct catagcgaaa ggggagtgtt actcggaaca
     3241 acaaataaat ttggatgttg aagattgcct tttttggaca gcagaaacgc ctaaattata
     3301 tacattactt ttaacatgta atggtgaata ttttaaagaa aaagttggct ttcgagcact
     3361 gacgatcaag aatcgtaagt tattgctgaa cggaacacct attaaattat atggagtcaa
     3421 ctatcatgac gtgaatcagc aaggcacgcc agtgatgacc cgagagcagt tacgtgcaga
     3481 tttgttatta atgaaaaaat taaatgtgaa tactgtacga acggcacatt accctaaatc
     3541 accagatttt tatgaattgg cggatgagat tggtctgtat gtaatcaatg aagcagattt
     3601 ggagtgtcat ggtgtggtta atctatatga tactgaagcg aattatcaac taattgctaa
     3661 tgatccgaca ttcgaaaagc cgattatgga ccgagtgatc cgttctattc ttccttatca
     3721 aaatcgaagc tctatcatta tgtggtctat ggggaatgaa tctggttatg gagtaaattt
     3781 tgaaaaagct caagctttag cacggaaatt ggatgattca cggttgattc ataatgaacg
     3841 ctcgattgaa ccagttattg accaagtgaa tgattttagt aacttggatg ttattagtca
     3901 aatgtatccc ccactggaag atgttcatcg tcattgtcaa agaagtgata ctgaaaaacc
     3961 tttttttatg tgtgaatata gccacgctat gggcaacggt cctggagatc ttaaaacgta
     4021 cttcgatttg attcaacagt atgattcctt tcttggcggt tgtgtctggg agtggactga
     4081 tcatgcaatc aatagaggaa ctttagaaac gccatcttat ttatacggtg gtgattttgg
     4141 tgaaaaatat cacgacggca atttttgcgt agatggttta ttgaccccag atcggcaatt
     4201 aacagtaggt gcattggagt accgtaatat tcattcgcct attcaactga ttgctgtaga
     4261 tgaggagctg caagaagtaa ccttgagcaa tataactgct tttacagccg gttcagcatt
     4321 taacctttct cttgaatggt atatagatgg aaaaaaaaca gtttatgaag taatgaatcc
     4381 tttcatactt gagcctggca aaagttgttc attgcccatc acagtaccta caacgattca
     4441 tggtgctcat acggttgctg tttcgctatc tgtttataaa aatgatgagc tttatggaca
     4501 gcaacagctc tttcttaaaa aagaaaaaat gagattgcca agaactgctc atcataatga
     4561 tgcaccacaa atttcagtaa aaggcacaga gctgataatc aaaggagaat cctttattta
     4621 tatatatcaa ttagcaaaag ctgcgttcac ttctgtaaag atcaatcaaa aggaactttt
     4681 ggaacgtcct actcaatttt caatttggcg tgcaccaacg gataatgatc gtaacttagt
     4741 aaacaaatgg aaagcagctg gatatgctga aatgtcttta gaaatcagaa aaaaagactg
     4801 gaaattcgta gatgatcatc tcctgatcga aacgtcttat cgaggagtta ttccaggatt
     4861 acagccaatt tttacaatgc acatacagtt gaaaataaat ggtgcaggcg taatcaacca
     4921 aaaagtatca gtggagaaaa atccgatttt tccagatttc cctcgctttg ggatggactg
     4981 gccgttgaat gatacttttt cgaaggtttc ttattgtggc tatgggccgt atgaaagtta
     5041 tcctgataag cactatgctg cgatgtttgg tatttatgag atggagattg aggaatttga
     5101 tcagccatat atcaaaccac aagaatatgg taaccgctgg caatgtgatt gggtatcaat
     5161 caaaaatcat caaggatcta ccatgacggt ttatggaaac tctaattttt ctgctctgcc
     5221 ttatagtcaa gctcaattaa ccgaaaagtc acaccattat gaattaataa aagaaaatag
     5281 aacttggttc aatttaagtt ataaacaaaa tggtatcggc tcacacagtt gcggtcctta
     5341 tttagaagat cgtttccgtt ttatggaaga tacttttgac tggaatatag tgattacttt
     5401 caaaggagaa aatagatgaa agaaacaaag atcaatcaag tcaaaaaaaa attatctcgt
     5461 tatcaagaag ttgtttatcg aaaccattgc ttaaaatggt caaaaaataa gctgaatgtt
     5521 cgttttaatg gattatttta tcgtttcgaa cagttagaag aagtctggaa accggtaacg
     5581 aatttccaat catgtcctgt acagctcata aatgatccaa aagaaacata cgatttatgt
     5641 ataacacacc tttataacca gcatttgaaa aatgacggat tttatttaaa ggttactaat
     5701 gacaatcaac ttacgattga atcagcaaac cttcgagggt ttcaatatgc tatggaagcc
     5761 ttattagaga gttttttttc ggtaggagat caattattat taccggaatt gattctgaaa
     5821 catgaaccat cagtgaaaat tcgaggaatc attgaagggt tttatggtat cccctggaca
     5881 catgaaatgc gcctctctct tttttcatat atgaaagata accaattgaa tacttttatg
     5941 tatgccccta aagatgatga attattacgt aaaaaatggc gtgaactcta cgatgcgcaa
     6001 gaattagaca aatttaagga gcttttatct gctgctgagg ctagcaatat cgatttttgg
     6061 tacttaatta gtcctggaaa tgatattgat attacgtgtg aagaagacat tcaagttctt
     6121 ctgaaaaagt tagaacaact gattgagctt ggcgtatttc aatttggtct attaatggat
     6181 gatattgact atcaattaaa aggtgctgct aaacgccgtt ttcgagagcc agcattcgct
     6241 catgcctatt tagtgaatcg agtagaggaa tatcttagca ctcggttgtc caatcatgaa
     6301 ttagtcattt gtcctacaga atatgataat cgtcatggtt ctcgctattt agcaacgtta
     6361 agtcaagaaa taccagaaca attaccgttc ttttggactg gcccaagcac gttagcggcg
     6421 tcaatttcta cagaagagct gcaggaaatg gcttctgtct atcaacggcc gatgctgatt
     6481 tgggataata ttccggtaaa tgattattta gaggataaag aactattgtt catgagtcca
     6541 tatgagaatc gaacaccgaa tctttctaag gagagatacc aggttacagg agtagtttcg
     6601 aatccaatgg ctcaattaga agcttcaaag tttacgatca atagtatggc taattactta
     6661 tggaactgtg agcgttttga tcctttggaa acttggacgt cagtcgttga aaaagtagtt
     6721 ggaagtaaac tacaaccagc ttatcttacc ttaaccaatg cttttccaaa tcattacaca
     6781 agcagtctgc tttcagatga agaattactg ctagccaaag acccagaatg ggtcacaaaa
     6841 gaaatggctg ctttagtcca agcagtaaaa tggatccagc aagaacaaca tacatctaga
     6901 ttgagaacag aattggagcc ttggttacaa gcaatcacag aaagttggac tttctggcaa
     6961 gaatggcaat taaaaaaaga tccaaaagaa gcagaagaat ggttggctaa gaaaaaaaca
     7021 caccgcatag gacgtgattt agtaactgcc catttagagc aaattatcaa aagttagata
     7081 tcttaaaaaa taaaaagcaa gaaaaaggag aacaaaatgg tcaagagatt aattagtgca
     7141 aactacagtg atgtcaagaa aatgacgtca aaagagttga agcaatccat caaagcgagt
     7201 gaaggacggg tgattgtttg tgaaacggtt gctaatactc ttccacaatc tggagaaatt
     7261 acgaatgcag aagttgcgtc agcatttggt gcagatttga ttcttttaaa tggtttcgat
     7321 tgttatcgtc ctgcagtttt tggactacca aatgtgacga ttgagaaggt ttttgatcta
     7381 cagcaaacga ttgaaaaccc gattcctaaa ttaaaagaac tagtcggtcg tcctgtggga
     7441 attaatttag aacccattgc ccctaaaaca aatatgatga gtgagaaagt agaaatcagc
     7501 caaggaagag caagctctgt tgaaacgata caaaaagcag aagaactagg agtcgatttt
     7561 atttgcttta ccggtaatcc aggcactgga gtaaccaatt tagaaattac taaatccgta
     7621 aaaatggcaa aaaagtattt ttcgggattg attattgctg ggaaaatgca tagcgccggc
     7681 tcagaagagc cagttgtgaa taaagaggcg attatttctt atgcggaagc aggcgcagat
     7741 attttactag ttccttcagt agggtctatc ccaggctttt tagagcaaga cctaattgaa
     7801 gcggttcggt tggcaaaaaa atatgatttg ttaacgatgt ctgcaatcgg tactagtcag
     7861 gaaagtgccc gaccagaaac attgcggcaa ttagccatta tgaataaaag ttgtggtgtt
     7921 gatttacaac atattggaga tgctggatac gggggattgg caccagcaga aaatattttc
     7981 gaaacctcgg ttgcaattag aggaatgcga cataccatca actgcgttag ccgctcgatc
     8041 aatcgctaac aaaattaaat aaaaaatggt taaattgatt atttaagtgg gaacatactt
     8101 gagatatcag ctaacttctg tcactttaaa aaagggactg tgacagaagt gaaaatagac
     8161 aaaaaatatc cgaacaagag actaggatag cagaaaaact atcgttttgc aacgaggacc
     8221 aactggaccg taggagcaat tctcttattg ttcggatatt tcgcttgttc aaggacttat
     8281 gtctgtagtg cccttttttg gtaggaaaaa tggtagggaa ccatatcatt tttaatgaaa
     8341 atcaagcatt tcaaaagt
//
LOCUS       DACDMM010000117         8329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDMM010000117 DACDMM010000000
VERSION     DACDMM010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.118"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            19..726
                     /locus_tag="IWD16_002090"
     CDS             19..726
                     /locus_tag="IWD16_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9089091.1"
                     /translation="MSPGINILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSV
                     SKYQESYKILQTIDQEKVSNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTE
                     TDIHHLQAAANVAMIYLSKGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYR
                     KIEDYDKAIQLCEKGIEYNKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIA
                     RFNGNDYAVKVIENDLK"
     gene            complement(999..1769)
                     /locus_tag="IWD16_002091"
     CDS             complement(999..1769)
                     /locus_tag="IWD16_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9089092.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            1987..3054
                     /gene="ulaG"
                     /locus_tag="IWD16_002092"
     CDS             1987..3054
                     /gene="ulaG"
                     /locus_tag="IWD16_002092"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP9089093.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            3072..3533
                     /locus_tag="IWD16_002093"
     CDS             3072..3533
                     /locus_tag="IWD16_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9089094.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            3553..5037
                     /locus_tag="IWD16_002094"
     CDS             3553..5037
                     /locus_tag="IWD16_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9089095.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            5080..5379
                     /locus_tag="IWD16_002095"
     CDS             5080..5379
                     /locus_tag="IWD16_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9089096.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            5394..6032
                     /locus_tag="IWD16_002096"
     CDS             5394..6032
                     /locus_tag="IWD16_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP9089097.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            6037..6897
                     /locus_tag="IWD16_002097"
     CDS             6037..6897
                     /locus_tag="IWD16_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP9089098.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            6887..7597
                     /locus_tag="IWD16_002098"
     CDS             6887..7597
                     /locus_tag="IWD16_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9089099.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            7829..8263
                     /locus_tag="IWD16_002099"
     CDS             7829..8263
                     /locus_tag="IWD16_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9089100.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
BASE COUNT         2740 a         1309 c         1785 g         2495 t
ORIGIN      
        1 aatatcgaaa acaagaatat gtcgccaggc atcaatatct taagtgcgct ttgcaaaaag
       61 ctgaatgtcg agatctccga tatattagta gaaacagaag aagacaaaat caggaaaaaa
      121 ataaatgaga ttcaatcatt gaattctgta tcaaaatacc aagaatctta taaaatttta
      181 caaacgatcg atcaggaaaa agtaagtaat tctgatcttt taaatcaaat tttattttat
      241 caaggactgc ttgatcatct ttatgcgaaa aaaccagatg cagcattatt ttattttaat
      301 caagtgttgg aacaaacgac cgaaacagat atccatcact tacaagcagc tgcaaacgtg
      361 gcaatgatct acttgtcaaa aggagaatta gattttgcga aagtttatgt ggagcgtaca
      421 cttaaaatat tgagtgagac tgattttgat aatctgatgg tctgcattgt atattatgat
      481 atcgccacct attaccgtaa aatcgaggat tatgataaag caatccaatt atgtgagaag
      541 ggtattgagt ataataagaa acataaatcg acttatgcat tggaatactt gctttatgaa
      601 attgcttctt gccataagca attaggagaa gatgattatt tagaaagata tatggacgca
      661 aaaaaaatag cgcgtttcaa cggaaatgat tacgcggtaa aggtgattga aaacgacctg
      721 aaataaagtg gttgtgaaat cagtgttctg atttgaatac cgtttattcg aaaaaaggag
      781 gtgtgacttt tgtcatacct ccttttttcg atattgtttg attcctattt aggcaacata
      841 taacggttat aaaagaagca atagattatg atggtatcga ttggatggat acatcttggg
      901 attcctttat caatttgagc gtagcgtgtt tttcatttat cgataaattt gttattcatt
      961 tttttgataa tcctatttac atatttacgt cctggttctt aaaatgaatc tacttgaatg
     1021 gtttttatcc ccgtatttct gatctcctct aatacttttt cagaacaaaa tttatcagtg
     1081 atcaaataat caaagactga aatatcagca atcttgaact ttgatgtcag tccgattttt
     1141 cgatgatcag cgactaaaat cttacatttg attgtcttgt tgaccatcat ttcgttgatc
     1201 gttgattcat ttaatatctt tgttgtgacc ccatgtacta catcgactcc gctacagcca
     1261 atgatacaaa tgtctgcatt gactgaagaa atcgtattta atgcgatgtc gcccactagt
     1321 gcttcttttg gaaagcgcat ctctccacca gttaaaatat aactacaatt atctttgtgc
     1381 ggagagtaat tgattttcac gttgtttgta atgactgtca aatggttaga ttgaagaaaa
     1441 tccagtgttt tcaacgcagt cgagctagaa ttgataaaca gcgtatcata atcgttaatg
     1501 tatttactag cttctctggc aatcgcttgt ttaatccttt caggaccaga ttgatcaaaa
     1561 tcagtctggt ttttgtaatt ataagaacaa taaccgtaat tgcgtatgat ctcgtttttt
     1621 tcttctaata aatttaaatc tcttcgtatc gtactaatag atacatcaag ggattcagct
     1681 aattcgaaag ttgatagttg ttcagtctgc tgaagcagtt ccaaaatttc tttttgacgt
     1741 ttttcaatat tgacaagtga gtttttcatg acttttgcct cctgagggac atcttgatcc
     1801 atggatttat ttgactagtt gttcatttaa acattcatat ctgtctttat tatactgaaa
     1861 caatcattga ttactgaaag aaaatcatat aaaaaacaaa cggtcatata aaagggtttt
     1921 catggatgct gatacacatt aatatttact tgtaagttgg aacaacgata tggggggagc
     1981 aagcaaatga gtaaaactgt caatgaagta acaaaagaaa agtggttagt agaaacattt
     2041 cctgagtggg gcacatactt gaatgaagaa atcgaaacag aagtagtgga tcctggaact
     2101 tttgctatgt ggtggctggg ctgtacggga atttggctga aaagtcatga aggaacgaat
     2161 attttatgtg atctatggtg tgggacggga aaacgtagtc atgggaatgg catgatgaaa
     2221 aaaggccatc agatgatgcg tatgagtggc gttcaaaata tgcagcctaa tttacgtact
     2281 cagcctttcg taatcgatcc ttttgcgata aaaaatgtag acgctttaat cgttacacat
     2341 atccattccg atcatctaga tatccataca gccgcagcag ttcatcaaaa ttgtcctgaa
     2401 gcaacattca ttgggccaaa agaagtggtt cgcacgtggc aaaaatgggg aattcctgaa
     2461 gaaaaaacga ttacagtaaa gccaggtgat gcagtcaaaa tcaaagacat caatgtagta
     2521 gctttagaag cgtttgatcg gacagcgttg gtcacttgtg aagatccaga agaggtatta
     2581 aaaggaaaaa tgcctcagga gatggatcaa attgcagtaa actatttatt cgaaacatca
     2641 ggagggaatc tttatcatgc aggagattca cattactcca atttatttgc caaacatggt
     2701 aatgaacaca aaatcgatgt ctgtttaggg gcctatggtg aaaacccacg agggataaca
     2761 gacaaagtta cttctgttga tatgctgcgc atggcggagt cattgaatgc aaaagtagtg
     2821 attcctgtcc actacgatat ttggtctaat tttatggctg atccaaaaga gatcactgaa
     2881 atttggaaat tcaaaaagga tcgcctgaag tatggattta aaccttatat ctggcaagta
     2941 ggcgggaaat tcatctatcc gacaaacaaa gatgatcttg agtttaattt ttatcgagga
     3001 tttgaagatg tcttctcgat tgaaaatgat acaccgtttc cgtcgttttt ataagaaaga
     3061 aggggagttt cttgctgaaa tacttttttg accatcaatt gattcattac ccagataaag
     3121 aaatcacgga ttggcgtgaa gctatcgccg aaagctgtct tgtattgatc gataaggaaa
     3181 ttattgatga aacgtatgtg gaggagatca tccattgtgt agaaaaatac ggtccttata
     3241 ttgtgatcgc acctcaagta gcaatgccgc attcttcaga aaaaagtgat ggtgtttttg
     3301 gaacagctat ttcatttacc aagttgaaac agccaatccg ttttgaaagt gaaggggaga
     3361 atgaggagaa aacggctagt ttgtttttta cactcgcagc aaaagatcca gaagaacatc
     3421 tagaaaatat ccaaaaatta tccgaattat taatgacaga tggattggtt gagcgtctca
     3481 tctctacgaa ctccatcgat gattaccaag ttgtgatggc agaatataat taattgatag
     3541 aggagaaaaa agatggggga atttttgtta agcatttgga cgtattttgc aacaaatatc
     3601 ttgacgcagc cagcatacct gattggtttt atcgtgttat taggctatat tttgctgaaa
     3661 cgcccgcttc atgaatgcat cgctggattc ttaaaggcaa cggtcggtta ctttattttg
     3721 actgttggct ctggtggttt ggtcaataat tttcggccaa tcttggttgg actaaaggaa
     3781 cggttcaatt tggatgccat ggttacagat ccttattttg gacaaaatgc tgtggatgct
     3841 ggattaatgg agacatttgg ccgtaccttt ggagatgtga tgattttatt actgattgca
     3901 tttgttttaa atatcttatt agtccgtttt cagaaatata cgaaactgcg ttccgttttc
     3961 actaccggaa acgttcaggt tcaacaagca gctaccgctt tttggatttt actgttttgc
     4021 tttcctgatc taggtaggat acaagcattg atttttatgg gactgatttt aggttgttat
     4081 tggacagtag cttctaactt aacagtagga atcacgcagg aattgacaga aggtgccgga
     4141 tttgcagtgg cacatcagca aatgttcgga atctttatat ttgcaaaatt agctgagtgg
     4201 atgaaaaagc gggatgaaaa gaaaaataga tcaatcaagc aagacaaaaa aattgaagat
     4261 atcaaattac caggattctt gtcgattttc aatgagaata tggttgctac ttcgttactc
     4321 atgctatttt tctttgggat cattctgatt gtactaggca aagattattt gattcaagcg
     4381 caattcatgc aagaagggca gagtttctta ttctacatta tgacgacttc tttaaatttt
     4441 gcggtatatc tggcgatctt gcaattaggt gttcggacat tcgtcgatga actaactcaa
     4501 tctttccaag gaatctcaaa cacgatttta ccaggagctg ttccaggaat tgacgtagct
     4561 gctacttttg gctttggttc gccaaatgcg gtaacgattg ggttcttgtt tggtgctttg
     4621 ggacagtttt tgatgattgg tttactgatt ttattgaaat caccaacgat cgtcatagcc
     4681 ggtttcattc cgttgttctt tgataatgcc gttatagcag tttatgccaa taaccgaggt
     4741 ggattcaaag ccgcttgcct gttcccgttt ctttcaggaa tcatacaagt aggcggttca
     4801 gcattattcg ctacatggat tgggatgtca acatacggtg gttatatcgg gatgttcgac
     4861 tgggcaacag cttggccatt gtttacagta atcatgaaag tcctaggtta tgctggaatt
     4921 gccgtagttg tcattggttt attggctatt ccgcagttac aatatcggaa aaatcctgat
     4981 ggttattttt tgattgtaga agactatgac acatatgcag aaaagcagag agtataaaag
     5041 aaaatctgat tagacaacac tcataatgga gggattaaga tgagaatttt agtatcgtgt
     5101 gcaaatggat caggaacaag cttgatgatg atgagaagtg tggaaaaagc aatgaaagaa
     5161 ctaaacgtac caattaccaa aatccatcat tgtgccattt cagagggaaa aagttcagct
     5221 ggccaatacg atgtagtttt tacgccagtc aattttttga atatgtttac tcaggcagag
     5281 aaaaaaggag ttacagttat cggtatcaaa aatgtgatgt cagtcaaaga aatcagtgaa
     5341 aaatttgctg aaacaaagtg gtaccaaaaa aacaagtaaa ggaaggaact aagatgagca
     5401 ggccgaattt gcaaattgcg ttagatcaca atgacttaga acatgcatta ggagatgtta
     5461 tgaaagttgg tgatgtagta gatattattg aagtgggcac aattctttgc ttacaggaag
     5521 gacaaaaagc gattcgctgt attcgcagta tgttcccaga taaaaaacta gtcgctgata
     5581 cgaaatgtgc agacgctgga ggaacagtgg cgagtaatgt tgcaaaagca ggagcagact
     5641 ggatgacagt catttgttgt gcaacgattc caactatgga ggcagcacaa aaagaaatag
     5701 gtgaattgca agtagaactc tacggcaatt ggacttttga acaagccatg gattggcata
     5761 acatcggtat ccggcaggta atctaccatc aaagccgtga tgcgctgtta tctggtgaaa
     5821 cttggggtga aaaagattta tcgaagatca aaaaactgat tgaactaggc tttaatgttt
     5881 ctgtaactgg tggattaaat ccacacacat tacacttatt tgaagggatc gatgtgtata
     5941 cctttatcac tggtcgtggt attacagcag caaatgatcc gatgaaggcc gcacaaaact
     6001 tcaaagatga gatcattcgt atctgggggt aaggaaatgg ctcaaattgg gatttatgaa
     6061 aaagcgttac ccaaagatat ttcatggaaa gaacgttttt cccttgtaaa agaaatggac
     6121 ttcgatttta tcgagatgtc gattgatgag acggatgagc ggttagcacg attagattgg
     6181 tctgaagaaa agatagctga gctgagagaa gagatgttca gctccggtgt acgtatccat
     6241 tcgatttgtt taagtgcgca tcggcgattt ccgtttggtt ctgctgatcc agaaaagaaa
     6301 aaagcagcga aacaactaat gaaaaaagca atccatttgg cgcataatct aagcgtcaaa
     6361 gtaatccaaa tagctggata cgatgtttat tatgaagaga aatcaatgaa ttccagagaa
     6421 gactttatcg aaggaataaa agaaagcgta aaggaagctt ctaaatatgg catcattctt
     6481 tccgtggaaa tcatggatga tccatttatg aactccattt caaaattttt agaaatcaaa
     6541 gaacagattc attcaccttt tctacaagta tatccagacc taggcaattt gtccgcatgg
     6601 ccggaaaata atccggctcg agaacttgag aaagggatcg attatatcac tgctatccac
     6661 ttaaaagata cttaccctgt aacaaaagaa tattcaggcc agtttcgtga tgttcctttt
     6721 ggtagcggtt gtgttgattt tcttggatta ttaagacatt tgaaacgtct agattatgat
     6781 ggaacatttc tgattgaaat gtggagtgaa aaaagccaag actttcgaag agaaatccag
     6841 caggcaaaag actacttaag ttctaaattg aaggaggctg gctatgatgt cttgtgaaga
     6901 aaaaattgca gcaatgaagc aacgtgtcct tgaagcaaat ctggcattgc cgcgatacgg
     6961 attagtcaaa ttgacttggg gcaatgtgag tgaagtggac cgtgagctag gagtcatcgt
     7021 cattaagcca agcggtgtca gatatgaatg catgcaagca gatcaaatgg tggtaacaga
     7081 tttgtcggga aatataatag aagaagatag cttaaaacca tcttcagatc tccccacaca
     7141 tgttgttttg tatcaaactt ttgaagatat aactgctatt actcatactc attccacgca
     7201 ctccgtcatg tgggcgcaag caggacgcga tttgccagct tatggcacga ctcatgctga
     7261 tgctttttat ggtaaggttc cttgtacacg ccaattaacg aaagaagaag taagagaagc
     7321 ttacgaagtt catacaggga atgtgattgt cgagacgttt aaggaaagaa aattagatcc
     7381 aaacgaagtc cctggagtac ttgtctatgg acatggaccg ttcacatggg gggattcacc
     7441 tatgaaagca gtagaaaaca gtttgatctt agatgaaatt tgtctgatgg ccaaagaaaa
     7501 cgagctgatc aaccccaata tatgtgaaat cccgcaatat ttactagata aacattattt
     7561 tcgcaaacat ggaaagtatg catactacgg gcaatagaga tgctagggta agggtagaaa
     7621 attaaaagat aacacttgat agagagaaac agaggttgtg aagaaaattt atcacaacct
     7681 ttttatttat caaaaagtat gcattctaaa aggaaagagg aattttaagg aatcgagaga
     7741 atctttacag gagagttata tgtgatagat taaatagtca aagaactgta ttattagagg
     7801 gagaattgta taagaaagga agaaaaaaat gaatattgat tcccttatga tcgatgagat
     7861 ttctgaatta tataagagcg ttggttggac gaattacaca aaagacactg caaggttagc
     7921 tagagcattt gaacaatctg aatctctgat aaagcgtaat ggggaaggga agattatcgg
     7981 aatcgttcgt tggatcactg attttgcgac gatcgcattt attcaagata tcctcattca
     8041 tccgaggtat cagcgccaag gaataggcaa agtgcttttg aatgaagctt tggagaagat
     8101 cactagttat ggaccagtcc aaatcgaatt attgacagat gatactgaga aaacaaaaaa
     8161 attttatgaa tcagtaggat ttgttcaagt aaaggaaatg ggtggtattt cttatgtcat
     8221 agatactaga agaaaaaagg tgctttttgg taatgacaaa taacatataa taacgtacaa
     8281 aagaaaaaat tctagcttag ttctttagaa aaacaagagg atagactta
//
LOCUS       DACDMM010000118         8314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDMM010000118 DACDMM010000000
VERSION     DACDMM010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.119"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(630..2261)
                     /locus_tag="IWD16_002100"
     CDS             complement(630..2261)
                     /locus_tag="IWD16_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP9089101.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2275..3948)
                     /locus_tag="IWD16_002101"
     CDS             complement(2275..3948)
                     /locus_tag="IWD16_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9089102.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(3961..4278)
                     /locus_tag="IWD16_002102"
     CDS             complement(3961..4278)
                     /locus_tag="IWD16_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9089103.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4303..5703)
                     /gene="lacG"
                     /locus_tag="IWD16_002103"
     CDS             complement(4303..5703)
                     /gene="lacG"
                     /locus_tag="IWD16_002103"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9089104.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            5940..6725
                     /locus_tag="IWD16_002104"
     CDS             5940..6725
                     /locus_tag="IWD16_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9089105.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6728..7420
                     /locus_tag="IWD16_002105"
     CDS             6728..7420
                     /locus_tag="IWD16_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9089106.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7694..8065)
                     /locus_tag="IWD16_002106"
     CDS             complement(7694..8065)
                     /locus_tag="IWD16_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9089107.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2551 a         1820 c         1424 g         2519 t
ORIGIN      
        1 gattaggtgc aaaaattgca cttttattgc acttttattt ttatttatag tgttttactg
       61 ttctgtatat tgttttaact cccttgatag tatcgcattc tgttttatac actttacgtt
      121 gaaaacagag tgggaataaa gagccaacat aatatggagg gggaatccct ataaatcaac
      181 attcttcaat agctaaaatg cttgattttc attaaaaata agatggttcc ctaccaaaag
      241 agactataat ataagttaat ttgtttcatt cttatcataa aaagaagttc tcgaaaaacg
      301 aactgttttc cgagaacttt tttctatcca tctaagtgct caaatcatat tcacgacgga
      361 attctaaggt taccctttct taaacaaact ttatgttatc tgcacaactt tttgcaattt
      421 tttcattgct tcttacgaaa tcgttgaact ctctttagca taatacaaag tgtagatgaa
      481 gtctggtgct ttactcgttt tggtaaaaat gactggaaat cgtttctggt tatttttggc
      541 aaaggcgagg tgcaggttcg attcctgcct tctacgctaa cgatgtggct gaagggaacc
      601 tccgatcttg aatcgaaacg atccgcagtc tattgtttca ccaatgaaac aaaatcaacg
      661 ttcgtccaag catttcctga agccttgcta taaaccccaa tttcacaaac acctgttgta
      721 attggaatgt ctttgattgt aatttttgtc cattcacttg ttttaacaac cgcagtattt
      781 agttccgctc caccataatt tttgatatat aactgattag tatcaaactc tcccccagac
      841 attacatagg cactcaatgt gtaagttcct ttgcttatgc cactaacatt ttgataagct
      901 gatgcctcgt atgcttgatc ctgccaaaaa gttagtttgt agtccccaaa aaaagtgccc
      961 gcttccgttt tgatcgtacc cttagcggtt ccttgttgaa accaggtttg ccaattggaa
     1021 ggactggtcg ttggtccatc gctttcgaaa cttgcatttt taactagatt tatttgcgcc
     1081 cctgcttgtt taaacgcacg caagcttggc aaggcgtttc cagcaaaatc aaacatcgct
     1141 tggttgtccc attcgtttcc catagtggat tgaacactga gataattcat acctgcttgt
     1201 gttgcccaac tcacatttcc gttgtaccaa agaggttccc aatagaagaa gcccgccccg
     1261 cgattattcg gtactgcctg aatccgatcg gcaagatcca gcaaatactc tgcttgtcct
     1321 gtcggtgttg gttcatatcc ccccgcagct gcttcggctt caccaaaagc atttggcata
     1381 gtatcggcat tcgctgttgt atgcccataa gctgtttcaa ctacgatcac ttctttacca
     1441 taccgttggc tgatcgcatt catattggtt tgaagttcat tcattgaacc gtgccaatac
     1501 ggataatacg acactccaat taaatcaaat gacacacctc gactcgtgat ttcatcaaac
     1561 caccaagtaa atgctcctgt atcgccacca tcagctaagt ggagcattac tggagttgtt
     1621 ttaggttgag tgtctcgaac agcttggata ccagacttca aaaaagcagc caatcgatca
     1681 aattctcccc cgccttctcc ccaagacttg ccataaggcc agagcattcc gctattcagt
     1741 tcattgccga tctgaaccat atcaggataa acgtccagct gcttcatttc cgacagaacg
     1801 ttctttgtat agtcgtacaa tgccgtattc atttcttcaa aggtcagacc ttgccatgct
     1861 ttaggaagat tctgtttccc aggatcaacc caaaaatcac tgtagtgaaa atccagcagc
     1921 accttaagtc ccagattttt tgctcgtttt gcaaggtcaa tcgtcgtttc cagatcattt
     1981 gtccctgctc cataactttc tccattacta ttataaggat cctgccacaa acgcagtcga
     2041 acatagttga caccattatt ttttaaaatc tctaaggcat cttttgaaat atcgtcttca
     2101 taatattttg caccagattt ttccatatct gacaggatcg aaatatctgc tccgcgaata
     2161 aattcgctgg cagatgcttc aacagttttt gaaacagccc ccaaagcccc taacacagct
     2221 gcaaataaaa cagctttacg caaccaaatt cccattttca ttatggctcc tcctttattg
     2281 aaattggttc agaataaact gaacagcttt ttcaggatct ctcgttaatc cgatatattc
     2341 tgctcctttt gtcgcaacca gcttgattcc taagcgatcg gtgtcttctt tgatatcatc
     2401 atagtaagaa ttgacttgcg gtgctaagac caccatatca aaatcctgca gcatttcgta
     2461 atgagaacca taagcacccg ccattgcact gatcggtgct ttcgattcag atgcaccttt
     2521 ttttaaagca ttagctaaca tcgcacttgt ccctgcgcca gcacaaagta ccaacacatt
     2581 gatttcttta tttgcatcgc tgataacttg actcgctgcc acaggtactt cctcacgtac
     2641 atcggcagaa gcaccttcct cggttgcgat cacctctgct ttttgtgctt ctttctctac
     2701 taagacagca tcgtatgctt tgcaaaatgg tagatagatc aggaagtcaa cgacgattag
     2761 aaccgctgct agaacaaaag ctaagatacc catgcccgtt cctagaacca aaccgatcgg
     2821 tgctggagtt gcccaaggca aaacgtacat aaagctgttc atgcccaata catcgacaaa
     2881 aaacttgaac aggcaaacgt ttacaacagg tgttaacaaa aatggaataa aaaaataagg
     2941 gttcaaaatc atgggtgccg caaatagcaa tggttcgttg acagcgaaac ttactggaat
     3001 aaaggaagct ttcccaactg ccttcaactg cttagactta gcaaagagta ggaagataaa
     3061 aggaacaacc aatgttgccc cagttcctcc catagttcct acaaaattcc ctaaacccac
     3121 cgtcagtact ttagaagctt gttcaccgga attgaacaat tgcagattgg tttccacatt
     3181 ggcatagata attgccgcaa ttgccggctc cacgatcgac ggtccatgaa caccgacaaa
     3241 ccaaaacagc gccatcgctc cccagataat agcgattcct aagtacccgt cagctgctgt
     3301 aaatagcggc tgcagcaccg taatgattgc ttctgcaaat gagatttgga agatttgtcg
     3361 aatgcctaaa tcaattaaaa cggccgcaaa tactgaaaaa gagaaaggaa aaatatctcg
     3421 gaaggtttgc gaaatcgttc caggaacttc tttaggcatt ttaatggtaa tatctttcag
     3481 cacacaaaac ttatataaat taactgttaa aaatgcagcg acaaaggaag ccagtagtcc
     3541 ctttgtcccc atatattccg ttgaaaatcc accttcaatc ggagagacgc ttaacaacag
     3601 gaaacttacc acagaggcca gcatcacaga ggtggtgttg attactttgt ttttcggtaa
     3661 gcgacgattc atcgaatcag ccaagcagcg agcagtggtt gctgaaacga gtaaccctac
     3721 aacacccatc gaataattat agactttcca tagccatgtg gagacagatt caggccattg
     3781 gtaaccaaaa atctcaggaa ccgcagcaat caaaatgaaa atgcttgaaa ataaaatagc
     3841 tggcatcgct gttaaaaatc cgtcacgaac ggctcctaaa taactattac gtgagatttt
     3901 ttcaaaaaat ggtttccctt tttcgatttg tttaatgatt ccatccatag cgttcttctc
     3961 ctatccttga ttctgtctgt acagtacaat aaagtcttga atcagatctc tcagcaataa
     4021 agtggtcatt aaatgatcct gcccatggat aaaaatgaat cccatctcca tctctttccc
     4081 agatgcttct tccgctaaaa tttttgtttg cgaattatgt gcaagggtta aattttcgtc
     4141 ggcttctttc aacgccgaat caacatgttc aaagtttcct tgtcttactt ctttcaataa
     4201 tttcaataat gtactgcgag cgtcgcctga atatgcaacg atctcaaaac caagcatttg
     4261 taattcctct ttattcatct tttcttcctc cattatcact tattaaataa tcgttttggt
     4321 ttcactgacc aacttgtacc agtaagctga ttcttttggg taacgcttct gggtttcaaa
     4381 atcaacataa aacaagccat accttttgtt atacccattt gtccatgaga aaagatccat
     4441 aagcgaccac aaaaaatacc ccttcacatt taccccggct gttatcgcct tgcttaagga
     4501 ttccaaatag actctcaaat agtcgattct cggctgatcc ataataatgc catcttcaaa
     4561 ctgatcttta taccccatcc cgttctcagt gatatagatt ttgttgtaat gtgggtaatc
     4621 gcttttaatt cgcagcaaca agtcatatag accttctgga taaatcagcc agtcccaatc
     4681 agttcgagga attccttctt tataaatacg ttctccgatt cccttcactt tataaacaga
     4741 ggtccctttt tcacccgtac cgttatgatg gattgcattt tctccatcgt aagctttgac
     4801 aaaatggcat tggtagtgat tgatccctaa gtagtcatta cgcgtggatg cttttttcaa
     4861 ttcaacgaaa tcttcttcag gaaaatgata gctggcctga tttgcttcac atatctcatc
     4921 aagagcagtc aaggtttcag ttgaataata gcctaaatag gtagcatcta ataagaaacg
     4981 gatcgacaat gcatcgtcta aaaaggcagc atgcttatct tccggcgcgt ctgttgccgc
     5041 atatttcgtc tctaatgagt gaactacacc aatttcaccc ggaagttcgt tttctttgaa
     5101 atagttcact acacgggcat gagcaaccat catattatga agacaggcga cgatctttgt
     5161 aaaatcatat ttgattcctg gtgggaaaac acctaacaaa tattgattgg ttgcaactgg
     5221 atagatttca ttaaaggtac tccacacctt gacttctttg aattcatgaa aacaaaaaat
     5281 ggcataggaa acaaatgctt ctattgtttc tcgattcaaa aaatctccat ggtcaaacaa
     5341 acgtttaggg gtatcaaaat gatgcaacgt cacaaacggc gttacgtttc ttttcttaca
     5401 ttcttcgaac acacgatgat aaaaagcgat tccttctgga tttggtttcc cagctccatc
     5461 aggaaaaatg cggctccaag caatggataa tcttagacca tttacaccaa aacgttcaca
     5521 caattcaatg tcctttgcat attgatgata aaaatcactt gccggatcgg gactgaatcg
     5581 cccttgttcc tctaaaaagt catcccaagc aacagcgcct ttgccacctt ccttagtggc
     5641 tccttcaacc tgataggctg ctgtggctcc gccaaaaata aaatcttctg gtaatttaag
     5701 catgtacatc tctcctcaaa tctataatca gttttcgctc tttctaactg attgcattgt
     5761 aaacgcatcc aaaacatttg tcaacaaaaa caaacacaaa taatcataaa aaccagaaaa
     5821 cagcaataaa atctattgta acaaacataa aaaccaccct aaatgtttac ttttgttcga
     5881 attagattga ctgttgtccg tttatatgat agattatcct taaattagag gtgaagacaa
     5941 tgttgaaaaa agaaagattg cttactatcg tagaaatggt caataaaaaa ggaatcctta
     6001 ccgttaatga aatcatcaat caattagatg tttctgatat gaccgttcgc agagatttag
     6061 atgaattaga aaaatcgggc aagctgctgc gtgtccatgg aggggctcaa agtctttcct
     6121 acactttaga tcaagaactt tctcacactg aaaaagccac tctgcagatt caagaaaaaa
     6181 aagaaattgc tgccacagca gcctccttga ttcacgaggg agaaacgatt ttcttaggac
     6241 caggtacaac aatcgaacta ttggcgacgc atttactgaa caagaaaatt cggatcatta
     6301 caaataatta tccagttttt gatattcttg ctcaagcaga tggaccagaa attctactga
     6361 ttggcggtga tttccggaaa aataccggcg catttgtcgg accgatcacc aacgacaatt
     6421 taagaagact aaagtttaca aaagcattta tcagttcaaa tggcgttcac aacgaagcga
     6481 tctctactta cagtaccgaa gaaggagaag cacagcagat cgctctaaat aattcccgta
     6541 agaaatatct actcgttgat aataaaaaat tcaatcgaga tgatttttat gtgttttata
     6601 atttgcatga tttcgatctg ctcattacgg atcgccagat ctcgcgggag gttcaagagc
     6661 actatgcaca atatgtcgat ctgcaaattg ctgattcaac cgaaaatacc actataggag
     6721 cgtaactatg aaagggatta tatttgattt caacggaaca atgtttttgg actctcatct
     6781 gcatgaagct gcttggctgc atatgataca tgatcataca aaagatggtt tatcagacga
     6841 ggatatttta aaaaatattc atggtcggac gaatacggag attttgaaac atttcatttc
     6901 agagcatttg accgcctctg agatcgccgt attgtcagaa gaaaaagaaa gctattatcg
     6961 tatgctttgc ttgcaaaatg aaaacgagct tcagttgaca aaagggcttg aagcgactct
     7021 tgaccagctg actgaccaac agattccatt aacgattgcc acggctactg tcaaagagaa
     7081 tgttgctttt tattttgata ttttcgattt agcccgctgg tttgactggg acaagattgt
     7141 ctatgacgac ggctctttcc ctgggaaacc gcaaccggat atttttctta aagcggcaga
     7201 caaactgagc ttagctccgg ctgattgtct tgttatcgaa gatgcttact caggattact
     7261 tgctgccaaa cgtgcgggga tcggaacaat tattgcaatt gatccattcg gtaaaaacaa
     7321 aacagtattt gaacaggcac agctaaataa agatggaacc atcaaagatt ttactggttt
     7381 ttggacagag cacctgtctg ccacatccgt taattcttaa acgattcaac taactaccaa
     7441 agaaaagcaa ctgaaatgca cacctccttt tataaaaaac tgccagtgaa acgacatgaa
     7501 agatacactg ctatatcctt agtgaaatat agcagtgtat ctttgacttt ttcattcttt
     7561 taaccatatg acgcgttcat ctctatctat aattaacatt aaaaatagtg ttttcacatt
     7621 atttctcaaa ccaatcttaa aaatcatcag gagtactttt ttgatatact tttattaatg
     7681 ctgggcgtaa tggttaaaag atttccacta gttcctctcc accaagctga gttgaaaaat
     7741 gcttatctgt aaacaacata gacaaagagg aaataaacgt ttccgtatct ttctctgtcc
     7801 caaggatatt aattaatttt cctactaggt aagtatcatt aaacttaaga tctgccttca
     7861 catcaaaagt tttgataaaa gcttttatca tgcgtacaat tttgtcttta tcgccataat
     7921 tgatttcacc actcatatca atatagtcac taatccctga agaaataatg gcaactgctt
     7981 cttctttcgc cttatcagat aattcattta atgactggga ttgattgatg acttcttgag
     8041 ttttatctgt tacgtaatct ctcattatcc tatcggcata ctccgcaaag taatcttgaa
     8101 aaaattcttc tacagggtaa ccaacttcgt tttcccaacc aggagaaatt tcattatcag
     8161 tacttattaa atctattaaa aaccaacccg ttctctgagt gttgattcta taatattttt
     8221 aatttcctat ataaatcaaa aatattatag aatcaataag ctaattaaga aacaaacttt
     8281 agaaactcta gaagaacttc tgaatttata taga
//
LOCUS       DACDMM010000119         8262 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDMM010000119 DACDMM010000000
VERSION     DACDMM010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.120"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            224..649
                     /gene="lacA"
                     /locus_tag="IWD16_002107"
     CDS             224..649
                     /gene="lacA"
                     /locus_tag="IWD16_002107"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP9089108.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            666..1181
                     /gene="lacB"
                     /locus_tag="IWD16_002108"
     CDS             666..1181
                     /gene="lacB"
                     /locus_tag="IWD16_002108"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP9089109.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            1192..2124
                     /gene="lacC"
                     /locus_tag="IWD16_002109"
     CDS             1192..2124
                     /gene="lacC"
                     /locus_tag="IWD16_002109"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP9089110.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            2127..3107
                     /gene="lacD"
                     /locus_tag="IWD16_002110"
     CDS             2127..3107
                     /gene="lacD"
                     /locus_tag="IWD16_002110"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9089111.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            3135..3452
                     /locus_tag="IWD16_002111"
     CDS             3135..3452
                     /locus_tag="IWD16_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9089112.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            3452..5158
                     /locus_tag="IWD16_002112"
     CDS             3452..5158
                     /locus_tag="IWD16_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9089113.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            5300..6706
                     /gene="lacG"
                     /locus_tag="IWD16_002113"
     CDS             5300..6706
                     /gene="lacG"
                     /locus_tag="IWD16_002113"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9089114.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            6729..7001
                     /locus_tag="IWD16_002114"
     CDS             6729..7001
                     /locus_tag="IWD16_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAP9089115.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            7022..7921
                     /locus_tag="IWD16_002115"
     CDS             7022..7921
                     /locus_tag="IWD16_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9089116.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         2617 a         1411 c         1703 g         2531 t
ORIGIN      
        1 gatggattaa atgaacaaaa atgaacaata atttaacggt gttatctatt ttttaaaaaa
       61 acaaataaaa aaaacaaaaa ataaacaaaa aaagtgttgc tttttgtttg aatgcttgat
      121 attatataaa caaagaaatg atgaaaacgt tttttcgaac attttgcaaa atgttttcta
      181 cttctacgta gcatttcttt ctaaaattta ggaggtggtc aaaatggcta ttgttgttgg
      241 tgcagatctc aaaggtacga gattaaaaga tgttgtgaaa aattttcttg tagaagaagg
      301 ttttgaggta attgatgtga ctaaggacgg acaagatttt gttgatgtta ccttggcagt
      361 tgcctctgaa gtaaataaag atgagcaaaa cttaggtatt gtaattgatg cttatggagc
      421 tggtccattc atggtagcaa ctaaaattaa aggtatggtt gcagcagaag tttcagatga
      481 acgctcagct tatatgacac gtggacataa caattcacga atgattacgg ttggcgctga
      541 aatcgtcggt gatgagcttg ctaaaaacat cgctaaaggt ttcgtcaatg gtaaatatga
      601 tggcggacgt catcaaatcc gagtagatat gcttaacaaa atgtgttaag aaaaggagtt
      661 aaggaatgag aatcgcaatt ggatgtgacc acattgtcac agatgaaaaa atggcagtat
      721 cagaattctt gaaatcaaaa ggacatgagg tccttgattt cgggacatat gaccacgcac
      781 gtactcacta ccctatctat ggtaaaaaag tcggtgaagc agtagtaagt ggtcaagcag
      841 acttgggagt atgtatctgt ggtacaggtg tcggcattaa caatgcagtc aataaagttc
      901 caggtgttcg ctcagcgttg gttcgtgata tgacatcagc tatatatgca aaagaagagt
      961 taaatgcgaa tgttatcggt ttcggtggaa agattactgg tggtcttctt atgaatgaca
     1021 tcattgaagc tttcattgaa gctgagtaca aaccaacaga agaaaataaa aaattgattg
     1081 caaaaatcga acacgttgaa acacataatg cacatcaagc agatgaagat ttctttacag
     1141 aattccttga aagatgggac cgtggtgact accacgatta aggtataaca aatgattctg
     1201 acagtcacac taaatccttc agtagacatc tcttatcctc tagaaacatt gaaaattgat
     1261 acagtgaata gggtgaaaga tgttagtaag acagctggtg gaaaaggctt aaatgttact
     1321 agagtcttgt atgaatctgg tgataaagtt actgctacag gtttcttagg tggtaaaatt
     1381 ggtgaattta ttgaaagcga attagaacaa tctcctgtta gtccagcttt ttataaaatc
     1441 tcaggaaaca ctagaaactg tattgctatc ttacacgagg gtaaccaaac agaaattttg
     1501 gaacaaggac caacaatttc tcatgaagaa gcagaaggct ttcttgacca ctatagcaat
     1561 cttattaaac aatcagaagt tgtgacaatt tcaggaagtt taccttcagg acttcccaac
     1621 gattattatg aaaaacttat ccaacttgct tcggatgaag gagtagcagt cgttttggat
     1681 tgttcaggtg cacctcttga aacagttcta aaatcgtcgg ctaaacctac tgccatcaaa
     1741 cctaataatg aggaactttc acagttgctt ggaaaagaag taacaaaaga tattgaagaa
     1801 ctcaaagacg ttcttaaaga gtcacttttt agtggtatcg aatggatagt ggtctcatta
     1861 ggtcgaaacg gtgcctttgc aaaacatggt gatgttttct ataaggtaga tattcctgat
     1921 atcccagttg ttaatccagt cggatcaggg gactcaacgg tagctggtat tgcatcagct
     1981 ttaaatagta aaaaaagtga cgctgactta ttaaaacatg cgatgacatt gggtatgtta
     2041 aatgctcaag aaacaatgac agggcatgtg aatatgacta attacgaaac actaaactca
     2101 caaattggag taaaagaggt ataaaaatgg tacttacaga acagaaacgc aaatctttgg
     2161 aaaaaatttc agataaaaac ggtgttatct cagctttggc atttgaccaa cgtggtgctt
     2221 tgaaacgttt gatggcacag tatcaagata cagaaccaac tgtagctcaa atggaagaac
     2281 taaaggtttt ggttgctgat gaacttacaa aatacgcttc ttcaatgttg cttgaccctg
     2341 aatatggact tccagcaaca aaagcattgg ataaagaagc aggtcttctt cttgcctatg
     2401 aaaaaacagg ttatgataca tcaagtacaa aacgtttgcc tgactgcttg gatgtttggt
     2461 ctgcaaaacg tatcaaagag caaggcgctg atgccgttaa gttcttgctt tactatgatg
     2521 tagacagctc agatgaactt aaccaacaaa aacaagctta tattgaacgt gttggttctg
     2581 aatgtgtcgc tgaagatatt cctttcttct tggaaatcct tgcttatgat gaagaaatct
     2641 cagatgcagg ttctgttgaa tatgcaaaag tgaaacctcg caaagtcatt gaagcgatga
     2701 aagttttctc tgatccacgc tttaacattg atgttcttaa agtagaagta ccagttaacg
     2761 ttaaatatgt cgaaggtttt gctgatggtg aagtagttta cagtaaagct gaagctgctg
     2821 acttctttaa agcacaagaa gaagcgacta accttccata catctaccta agtgcaggtg
     2881 tatctgctaa attgttccaa gaaacattgc aatttgctca cgactcaggt gccaagttta
     2941 atggtgtgct ttgtggacgt gctacttggg caggatctgt agaaccttac atcaaagaag
     3001 gtgaaaaagc agcacgtgaa tggttgcgta ctactggatt cgaaaatatt gatgaactta
     3061 acaaagttct tgttaaaaca gctagtccat ggactgataa agtatagtaa aaacataaaa
     3121 cggaggatat tgttatgaac agagaagaga tgactctctt agggtttgaa attgttgctt
     3181 atgctggaga tgctcgctct aaacttttag aagcgcttaa agcggctgaa aatggtgatt
     3241 tcgctaaggc agatagtctt gtagtagaag caggaagctg tattgcagag gctcacaatt
     3301 ctcagacagc tatgttggct cgagaagctt ctggggagga acttccatac agcgttacca
     3361 tgatgcatgg tcaggaccac ttaatgacta ctgtcttatt aaaagatgtg attcatcacc
     3421 tcatcgaact ttataaaaga ggagcaaaat aatgcataaa ctcattgaac ttattgagaa
     3481 agggaaaccg ttctttgaga aaatctctcg aaacatctat cttcgtgcta ttcgtgatgg
     3541 atttatcgct ggtatgccag tcatcctttt ctcatctatc tttatcctta ttgcctatgt
     3601 cccaaatgct tggggattcc actggtcaaa agacattgag accttcttga tgactcctta
     3661 tagctattcg atgggtatcc tagcattctt tgttggtggt actaccgcta aagctttgac
     3721 ggactcaatg aaccgtgacc tacctgcgac caatcagatt aacttcttat ctacgatgct
     3781 agcgtctatg gtagggttcc ttttgatggc agctgagcct gcaaaagaag gaggcttctt
     3841 gacagccttc acgggaacaa aaggtctttt gacagcattt attgcagcct ttgtgacagt
     3901 taatgtttac aaggtctgtg tgaaaaataa tgttactatt cgtatgcctg aagaggttcc
     3961 accaaatatc tctcaagtat ttaaagactt gattccattc actgtagcgg ttgttctcct
     4021 ttatggattt gaactcattg ttaagggaac tcttggtgta accgttgcag aatcaatcgg
     4081 tacccttctt gctcctctct tctcagctgc agatggttac cttggaatta cacttatctt
     4141 tggtgcttat gccttcttct ggtttgttgg tatccacggt ccttcaattg tcgaaccagc
     4201 aatcgctgct atcacttatg ccaatatcga tgccaacttg catcttatcc aagctggaca
     4261 acatgcagac aaagttatca cttctggtac tcaaatgttt attgttacca tgggtggaac
     4321 aggagctaca ttgattgttc cattcttgtt catgtggatt tgtaaatcag aacgtaaccg
     4381 tgccatcgga cgtgcctcag ttgttccaac attctttggg gttaatgagc caatcttatt
     4441 tggtgcacca atcgtattga atccgatttt ctttgtaccg ttcattttcg ctccgattgt
     4501 caacgtttgg atctttaaat tctttgttga cacattgaac atgaactcat tctctgccaa
     4561 ccttccatgg gttactcctg gtccgttggg aattgtactc ggtactaact tccaagttct
     4621 atcatttatc ttagccggac tcttggtagt tgttgatact atcatttatt acccatttgt
     4681 taaggtatac gatgaacaaa ttcttgaaga agaacgttct ggtaaaacaa acgatgctct
     4741 taaagaaaaa gtagctgcaa acttcaatac tgctaaagca gatgccgttc ttggaaaagc
     4801 aggcgttgct aaagaagatg tagcagcaaa caataatatc acaaaagaaa ctaatgtcct
     4861 tgttctttgt gcaggtggag gtacaagtgg cttgcttgcc aatgccttga acaaagcagc
     4921 agcagaatac aatgttccag tcaaagcggc agcgggtagt tacggtgctc accgtgaaat
     4981 gttgccagag tttgacttgg ttatcttggc tccacaggtc gcttcaaact tcgatgatat
     5041 gaaggctgaa acggataaat tgggcattaa acttgcaaaa actgaaggag ctcaatatat
     5101 caaattgaca cgtgatggac aaggtgctct tgcatttgtt caacaacagt ttgattaata
     5161 aatttgtaaa agttatttga gacgagatta ttctttcaat tccttataat attgttaacc
     5221 atatcattgc atttcatttc cttggtttcc ggaactcaga atatcgtctc aaatgctttt
     5281 tttgaaagga tttatagtta tgactaaaac acttcctaaa gattttattt ttggtggtgc
     5341 tacagctgct tatcaagcag aaggtgcgac tcacacggat ggtaaaggcc cagttgcatg
     5401 ggataagtat cttgaagaca attattggta cacagcagag cctgcaagtg atttttacca
     5461 taaatatcct gttgatttag aattagctga aaaatatggc gttaatggta ttcgtatttc
     5521 aattgcctgg tctcgtattt tcccaactgg ctatggcgaa gtaaatgaaa aaggtgttga
     5581 attctaccat aatctttttg ccgagtgcca caaacgtcat gttgagccat tcgtgacttt
     5641 gcaccacttt gacacaccag aagttcttca ctcagatgga gatttcttaa accgcgaaaa
     5701 tatagaacac tttgtagatt atgccgcttt ctgttttgaa gagttcccag aagtaaacta
     5761 ctggacaact ttcaatgaaa ttggcccaat tggagatggt caatacttgg ttggtaaatt
     5821 ccctccaggc attcaatatg atcttgccaa agtcttccaa tcacaccaca atatgatggt
     5881 ttctcatgca cgtgctgtga aattgtataa agataaaggt tataaaggcg aaattggtgt
     5941 agtacatgcg ttgccaacca aataccctta tgatccagaa aatccagcag atgttcgtgc
     6001 cgctgagtta gaagatatca tccataacaa atttatcttg gatgcaacct accttggaca
     6061 ctattcagat aaaacgatgg aaggggtcaa ccacatccta gctgaaaatg gtggagaact
     6121 tgatcttcgt gatgaagact tccaagcact tgaagcagct aaagatttga acgatttcct
     6181 tggtatcaac tactacatga gtgattggat gcaagccttt gatggtgaga ctgaaatcat
     6241 tcacaatggt aagggtgaaa aaggaagttc taagtaccag attaaaggtg ttggtcgccg
     6301 aatcgcacca gattatgtgc ctagaacgga ttgggactgg attatttatc ctgaaggctt
     6361 gtatgaccaa atcatgcgag tgaaaaatga ttatccaaac tacaagaaga tttacatcac
     6421 tgaaaacggt cttggataca aagatgagtt tgtagataat accgtttatg atgatggtcg
     6481 tattgattac gtgaagaaac acttggaagt tttatcagac gcaattgcgg atggtgcaaa
     6541 tgttaaaggt tacttcatct ggtcacttat ggatgtcttc tcatggtcaa acggatacga
     6601 aaaacgttat ggactattct atgtagactt tgacacgcaa gaacgctatc ctaagaaatc
     6661 agctcattgg tataagaaag tagcagaaac tcaagtgata gagtaatatt acttgaaaat
     6721 gtaaaaaatt gttatccgat actttagaaa aagtacaaca cttatttaga ccaggctttg
     6781 aaaaggtata tattgttagt ttcgaagatt gtcctatgat tcctgagctt gaagctaccc
     6841 cgttgctaaa atgtggtaaa tggtatgttt caactggaaa agagtggatt tgccattctg
     6901 atttagagtt atcagcattt gaatgggaat ttttacaatc actcgatgta gaaatacgtg
     6961 agactattca cttcgaggta aattatttgc ctttccaata ataaaaagaa aggaattagt
     7021 tgtgactatc gaattaaaaa acgagtatct tactgttcag tttaaaacac ttggtggtca
     7081 actgacttcg attaaagaca aggatgggat agagtatctc tggcaagctg atccaaatta
     7141 ttggaatgga caggcgccta ttctatttcc tatctgtggt agtttacgaa atgattgggc
     7201 tatttataga cctcaagaaa gacccttttt tacaggtctt attcgtagac atggttttgt
     7261 tcgaaaagaa gaatttattc tggaagaagt caatgataat agcgtgacct ttagtattaa
     7321 accaaatgct gagatgcttg ataattacct atatcagttt gaactgagag ttgtttatac
     7381 cttgaatggt aaatcaatta gaaccgaatt tcaggtaacg aacttggaaa tggaaaaaac
     7441 aatgccttac tttattggag cccatccagc attcaatagt cctttagtag aaggtgaaaa
     7501 ctatgaggat tattatattg aatttagtga ggttgagtct tgctcgatac caaagagttt
     7561 tcctgagaca ggattattag atttgcaaga tcggacacct tttttggaaa atcaaaaaat
     7621 tctcgatttg gactactcac ttttttccca tgatgccatt acacttgacc agctcaagtc
     7681 tcgtagcgta acgcttcggt caaggaagtc tggtaaaggt cttcgagtag attttgatga
     7741 ttttccgaac ttgattctgt ggtctacaac taataaaagt ccattcattg ctttggaacc
     7801 ttggagtggt ctctccactt cacttgaaga aggtaatttc ttagaagata aaagacaggt
     7861 aacgaaaatt tcacctcaag aaacttctag aaaaatctat gatattacta tccttaatta
     7921 aaatttgtta ttatttgagt cttcaataag atttgaatac tcttaccttg tgatgataga
     7981 ttggtcttct ccgtgccagt cttacatcgc aaggttttta agttttttgt gggtctatgt
     8041 caactgtagt gggtgacgaa aagctaatat ctaaagacag tctggtagta aaaaataaga
     8101 ttatttacta cttcttgatt taaaataata gtggagccat taatttgtgg caaatttaat
     8161 ttaatatttt gcctcaaatt agccacactt tttatagaac taagaggaaa aatgaaattt
     8221 tcttcctatt aatatagttg tttgaaatct taaatatagc gt
//
LOCUS       DACDMM010000120         8229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDMM010000120 DACDMM010000000
VERSION     DACDMM010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.121"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..417)
                     /locus_tag="IWD16_002116"
     CDS             complement(<1..417)
                     /locus_tag="IWD16_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP9089117.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEI"
     gene            complement(547..1257)
                     /gene="budA"
                     /locus_tag="IWD16_002117"
     CDS             complement(547..1257)
                     /gene="budA"
                     /locus_tag="IWD16_002117"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP9089118.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1270..2934)
                     /gene="alsS"
                     /locus_tag="IWD16_002118"
     CDS             complement(1270..2934)
                     /gene="alsS"
                     /locus_tag="IWD16_002118"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP9089119.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3142..3804)
                     /locus_tag="IWD16_002119"
     CDS             complement(3142..3804)
                     /locus_tag="IWD16_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9089120.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3814..4629)
                     /locus_tag="IWD16_002120"
     CDS             complement(3814..4629)
                     /locus_tag="IWD16_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9089121.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4889..5332)
                     /locus_tag="IWD16_002121"
     CDS             complement(4889..5332)
                     /locus_tag="IWD16_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP9089122.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5466..5804)
                     /locus_tag="IWD16_002122"
                     /pseudo
     CDS             complement(5466..5804)
                     /locus_tag="IWD16_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5792..6169)
                     /locus_tag="IWD16_002123"
     CDS             complement(5792..6169)
                     /locus_tag="IWD16_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9089123.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6393..7586
                     /locus_tag="IWD16_002124"
     CDS             6393..7586
                     /locus_tag="IWD16_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9089124.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7752..8174)
                     /locus_tag="IWD16_002125"
     CDS             complement(7752..8174)
                     /locus_tag="IWD16_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9089125.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2411 a         1669 c         1401 g         2748 t
ORIGIN      
        1 gatttcatca taccctttcg ttactccgca atcgatatct aaaacaccga ttaaattacc
       61 ttcttttacc attggcacta cgatttctga ttgtgcggct gaatcgcagg aaatgtaatt
      121 tgcatgggtt ttgacatcat caacgatcaa tgtttcttgt ttttcagctg attctccaca
      181 aactcctttt cccatcttga tacgtgtgca agaaactcgc ccttggaacg gtcctaaaat
      241 caattcatta ccatcgaata aataatatcc tgcaaagaca gtctcaggca aagtctcatt
      301 caacaatgca gatgaatttg ctaaatttgc taaccagtta tcttctgctt ctaataatcc
      361 ttgttgctgt aataataaaa tttcgtatgc atcttttttc tgtttttctg tccacatgat
      421 aaaaaccgct cctttttgat ctatggagaa gattatagct cagttttttt gtaaacgcta
      481 ggtttcctcc tcaaaaaaac gagatacgtc aggttaatca ccttgaacgc atctcgctgt
      541 ctttttctat tctgcttgtt ggatttctgc tgctaaatca gcataatcaa tcgtttcgtg
      601 aacaaaagtc ggatcatcaa ctggaaaatg ctgtacaagg gacgagatgc ggctaatctc
      661 aacttttcct tcagccactt caaaatccat tacatgtccg ccaaatgtat gtgtatcatc
      721 gataaaatgc aggtgaaatc ccgctgcagc aactccttgg aataattcag gcgtaaagaa
      781 cccgactaat gttccttgga tattgctttg ttggaattct ggttgagact ctgaaatttt
      841 tgctagtctt ctgtaaggtt tttcttgctt cggcatgata cggatgtgca tatttttgaa
      901 taatccagtt atcttcactg ccaaaaatag gttttccccg gcttctctta ctaatatgtc
      961 ttttttcaat tgttctccat ctgcagtttt tgagacagaa aaactagtat ctggcgtaaa
     1021 atctgtaatt gctgcatagg gggtaagttc acttccatct aattgggcaa ctgagccatc
     1081 tgatcttcct tgataagcta cgccatctaa aaagatgact tcaccatcaa gcccatgcaa
     1141 tgttcccaat cccagagttc ctttttctaa tatatgagca atcgtttctg ttccatccat
     1201 caagccggcc atcaaagctc ctaatgtccc gtgctggtat aagatttttt cagtcatgtc
     1261 gacacttcct taatagaatt gatctggtaa gattgtttct cccaatttct cattatcacg
     1321 gtaatcaatc ggaatatcaa taattacggg tccttctgtc gcaaatcctt cttctaaagc
     1381 tttttctaat tcctctacac ttgtagctcg gattccttta gcgccaaaag cttctgcata
     1441 tttcacaaaa tcaactggac caaagtctac acctgacgcc cgctgatatt tcattttttc
     1501 ttggaactct accatattgt agtgtccatc attccaaatc aaatgaacga tattcaattt
     1561 tttacgtacc gccgtttcta aatcctgggc agagaataaa aatcccccat cgccggaaac
     1621 tgatacgatt tgtgtatttg gacgcaccaa tgcagcagaa atcgcccatg gcaatgcaac
     1681 accaagcgtt tgcataccat tactgaataa caaatgacga ggttcataac ttctaaagtg
     1741 gcgtgccatc caaatataat gactacctac atctacggta acagtcatat catctgttac
     1801 ttttgactga agcgtgttaa taacttctaa aggatgcaag atacctgctt ctccttttga
     1861 ttgaacaatg tcccgctccg ttaattttgc ttgaagagat gctaaatatt cttttgcatc
     1921 ttctgacact tgttgaggct ctaatgaacc tgtcaataaa tcaagagtgg ctgagatatc
     1981 accgatcaat tctctttcag gctgcataaa aggatcgatt tcagctggcg cttcatcgat
     2041 cacaatgatc cgcgcatctt tttccgcatt ccagtttctt gcttcatatt cgattggatc
     2101 atagccaatt gcaataacta gatcacttcg tttcaaaagc atatctcctg gttggtttct
     2161 aaataaaccc acacgaccaa agaaatgcgc ttctaattca cgtgagatca ctccagctgc
     2221 ttggaatgtc tctacaacag gcaatgctgt cttttcaact aattgcctga tggctaacgt
     2281 ttctttttca cttgatcctc gcatacctac taatagtacc ggtagttttg cttctctaat
     2341 gcgttcagct aggtaacgga tatcatctgc agaagcagaa cctaatttag gatcgctcaa
     2401 tggcttgatg acattacctt ggacaggtgc atccactaca tcttgcggga tactgataaa
     2461 gctcgcgcct ttttttgaac tcttagccat acgataagca ttggcaatca cttctgaaat
     2521 tgtttctgga tcttggattt ctgcactgta ttttgtgatt ggctggaaca gtgctgcatt
     2581 atccatgctt tgatgggtca acttcaatag atcgcttcgt tttacttgtc cggcaattgc
     2641 taaaactggg tcaccttctg ctgtcgctgt taccaaacct gtagctaagt tactagcacc
     2701 aggaccactt gttgcgataa ctactcctgg ctctcctgta atacgtccga ttgcttgtgc
     2761 cataaatgca gcattttgtt cgtgacgagt cacgattaat tcaggacctt gatcctctaa
     2821 ttcattaaat acaccatcga tttttgcacc tggtataccg aatacataat caacatgatg
     2881 attaatcaaa ctttctacta cagatttcga accttgtttc attttttcag ccattgaaca
     2941 tcgtcctttt tatgctttat ttttaattat tagttagaaa taatcacaaa taagtgatac
     3001 agtttctaaa cctgtttcat acaaaagtat agcacgtttt atttcatttg tttagccata
     3061 tgtttcaaaa aatgaaaaag aatgacagtt tttacataaa aaaaactgtc attcttaatt
     3121 gaaagtttca atttataaag tctacagatc catagttctc atcaatttac gattgttgaa
     3181 gtaaatcaaa ccaaatacga tagaaggacc tagtagaata caccattggg gctttacgaa
     3241 aaagaagaga aacaaacttg tcaatagtaa aaaatctagt aaactcacat accatttttt
     3301 caacgtatag tagaacgcta tccgtagaac ttgatttggt ttcgacgctg gatttctcac
     3361 ctgaaaatac cagcagttgg tgctaaagga aactgataaa caagcaagta aaacaaacaa
     3421 cggaatcaac cattttccat aagaaaaacg tataaaaaag aaacaatcaa aaatcattat
     3481 cacactaact atccaagcaa atagccaata aaaaaatccc tttagccaaa attggcgaaa
     3541 catctttatg aacaaacgta ctggtgaaaa actattttct tttgctaatt cttctgtcat
     3601 acctatcatc acgatgcaac ctggaccaat aaaaagtaaa gaaaaaataa aggaaaagac
     3661 attccttcca tctatcgaca aaaggaacgc agctgctata tacaagatat tgacacacca
     3721 aaaacaaagg ctacttgcca atgattgata caaccaataa aagaattcca tataaacatt
     3781 tttgtcaaaa cgctgctctt tcataatcac ctcctattcc ttcattcctg atgaagcgat
     3841 cccttctaca aaatattttt gcatcgctaa aaatataaaa gcaacaggaa gaacagaaac
     3901 aactgaggca gccatgatta atgcgtactc tgcatcaaat agcccaataa acattttcaa
     3961 ccctaactgg attgttttat tttccgtaga agacaaataa atgaatggcc ccatataatc
     4021 gttccatgta ttaacgaatg tgatgatcgt caaactggca atcgccggtt ttgtaagagg
     4081 taaaatgatc ttgcgataga ttccccactc tgataagcca tcgatccgcg cactctcaca
     4141 taaagaatct gggatactac tgtaatattg cttcaacaag aacacaccaa aagcattgaa
     4201 cgcttgtaat agcaccaacg accacaacgt atctgttaaa cccacttttc tcatcataat
     4261 gaactgcggg atcatataag actgccaagg gacagcaatc gtgccgatat atgctaaaaa
     4321 taaaatatcg cgccctttaa aatttagttt agaaaacccg taagcagcaa aactagaagt
     4381 aaacaactga atcaacgtga tcacaacgct taaaaaagct gtattcttaa aaaatgtcac
     4441 taaaggaata cgagaccaaa taaccagata attctctaaa tgccaagttt tagggatcca
     4501 ttgaatcgga atcgtgaaca cttcattatt tgctttgaat gaagcagaaa ccatccataa
     4561 aaaaggaatg atcgtcaata aagctaatac aatcagcaga atcataccca accattgaat
     4621 aaagggcatg ttttttgaac gaatttttgc tttttttctt tcatttcgaa cacctccaaa
     4681 gttataatct tcatttcttt gtaatgatta caatccagaa caacggttat tatagctgat
     4741 tcatttccgt tcttcttatt tttgtaagcg cttatttaac aagttatgta aatctcttta
     4801 ttttttgatt taaaaatgaa aaaatacaaa agcaagactg tcatacgtct cgcttttgta
     4861 ttgaaatttc ttcgattaga gatacttatt actgttcaaa agcttctttg aaacggtgaa
     4921 tcaattcatt gactacaggt gataaaatcc gattcttttt ccagactagc atgcagttcg
     4981 tttcaatcat tgggtcaagc ggaacgaatg tcactcgctt tgaatgatga cttacagcac
     5041 cttctatgac taaagcagca cctacctgat tctcaattag cgcaaataca ttgaaaatca
     5101 aattgtacgt tcctgtgatc agcaactgct ccttttttat gcctaaccag tcagcaaaca
     5161 ttcgctgtat ttctggacgc tttgagatca aaagaggtac attcgcaaag tcttcttttg
     5221 ttatcacgtc tttttttgct aaaacagaat tttctaaaat gagcaatccc catttttcct
     5281 ttcttggtaa caaaatcgtt tcgtatttct ctgtatctac tggatctagc aaaatcgcta
     5341 aatctaacag gcttttttct agtcactccg tgatatcatc acttgttccg ctgtggatat
     5401 gaaattgaac gctgggataa tcacggatca acttttctag taatagtgcc aatgtattcg
     5461 aactatcagc ttccacacag ccgatcgtaa atcggccgct gaataattgt ttttttctgt
     5521 tctcgaattc ttgagtggtt tgattcgtta aatctaaaat ttcttctgct cggctttttt
     5581 agaaaataac ctgcttctgt caagatcatt ttgcggtttt cgcgtacaaa cagttctgtt
     5641 cctagttact cttctaactc cttcagttgt cggcttaagg ttggttgtgt aagatgtaga
     5701 acttctgcgg cttgagagat tgttccttct tctgcaattg tccaaaaata ttttagtaag
     5761 cgtaactcca tagcgatgct ccttgatttt tttacttgat acattccatt ttatttctag
     5821 ataacacacg atcatatttc ttgatttttc cttgtaaacg ttccaaacat tcttgcattt
     5881 cagcaatttt tttcgtcaaa agttcacttt cttcaatcaa aagcttacgc cgctcaaata
     5941 cagttgcttc tccttcgcta gataggcgaa tatattcaat caaagactcg atcgataatc
     6001 cggcatttcg catacagatc gtaaagtcga cccattttag gtcttcttct gtataatcac
     6061 ggattccgcc ttttgtccga ttaactggtg gaatcaaccc gattcgttca taatagcgca
     6121 atgtatcggc cgaaatgccc ttttcttcac tgacttgttt gatattcatc tgatcactct
     6181 ttctcttaat ttcttgccac tttacttatg tattttattt attctacaac tttaagtgta
     6241 ctccaagtca atactcattt ctattaaaac taagctggat tatctatatc ggttgtttgt
     6301 gtattatttc aaataaaaaa gtcttgtttt tttattcttt tttggatata cttttaacaa
     6361 tttagggaaa ttttaggaaa aggggtataa aaatgacaac gtatcagcga aaggtattag
     6421 tatcgacatc ggcgggaatt gctttagaga atatggatat tatgttttta gctttttcat
     6481 tatcttcaat gattacttct tttcatatta gtggtacgca agcgggtttc atttcaacga
     6541 tcactaattt ggggatgctc attggaggta ttttttttgg catcatagct gataaatatg
     6601 gacgtgtgaa agtattttct caaacggtcc ttctgttttc gattgcttct ttattgatgt
     6661 attttgcttc aaacatctac ttggtttatc tctttcgctt tatcgcaggt gtaggagcgg
     6721 gaggagaata tggggcgtgt atgtccttga tttccgaaag tttttcaaaa aaacaaattg
     6781 gacgagcttc ttcggttgct ggtattggtg cgcaggtcgg tgcggctttg gctgcgattt
     6841 tagctgctgt gatcattcca acgtttggtt ggaagatgtt gtatgtcgta ggtgtactgc
     6901 cggtgttgat ggtcttgttc attcgtcgcg gcttgactga accaaaggaa ttcaaatctg
     6961 tacaatctgc cgaaaagaaa accaaactta gcagactgtt tgaatcaaaa catcttgctt
     7021 ggcaaacatg tggccttagt atcatggtaa ctgtccaaat tgccggctat tttgggttaa
     7081 tgaattggct accttcaatt atgcaaaatc gattagggct ttcggtttct gggtcttcat
     7141 tgtggatggt cagtactatt ttaggcatgt ctttggggat gatgactttt ggcataatca
     7201 tggataaatt ggggcctaaa ctagcctttt ctttgttttt aatttgttca tccttatctg
     7261 tctatctact tgtgttagcc catagccagt ggagtttggt agcagccgct gtgatagtcg
     7321 gctactttat caatggcatg tatggtggat atggcgcagt gattagtagt ttgtatccaa
     7381 cagaaatccg cgcgacggcg aataatttcg ttatgaacct tggacgagct gttggtggtt
     7441 tttcttctat cgtcatcggt ttcttgatgg atcattatag tttaactgcc gtaatcttgt
     7501 ttttgagcgg tatttaccta gtcagtctgc tggtcttgac aactcttact ggtgttacag
     7561 cattaaggga aagcttgcag cattgatagt tgagtctgta aatgacaaga aagaggtgtg
     7621 gaaaaagctt gcatctgcgg taataagacc agccaaatca aaaacatgag acactgtttc
     7681 cgtttggctg gtcgtattac ttgatgcagg gcagcttttt caactgctaa tttccttcta
     7741 tctggcggcg actattcttt gacgtccgtc atttcaaagt actttactaa taacttagca
     7801 taacgaaaaa ctgtggatac actcgtatat tgagcgtctg cataatcttc caaatttttc
     7861 tcaggatgtt gatacatgaa ttcaatcaaa tgataattat atgattttct gtagaaatga
     7921 agataaataa tgtagattgg gaagttctcc tgcttttcca tagaaaagcg ccgattattc
     7981 tcattcttca aaatgacctt atcaggatat tgttggaaca attcttctaa atctgttaga
     8041 taattcaaga tagacttact tgataccttc aattcgtttt gtagttcttc tattgaacac
     8101 caacgttctg tttcaagcag taaatgaatc attttaattt tcttttgaga aacttttgtt
     8161 aaaaaagtct gcatattttc tcctcctgta tctactttac tatactacat acaagaaaaa
     8221 aacggatac
//
LOCUS       DACDMM010000121         8183 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDMM010000121 DACDMM010000000
VERSION     DACDMM010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.122"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            11..541
                     /gene="lepB"
                     /locus_tag="IWD16_002126"
     CDS             11..541
                     /gene="lepB"
                     /locus_tag="IWD16_002126"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9089126.1"
                     /translation="MNRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSMENVLKQGD
                     MVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKVVKEPYLT
                     KNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTINKTDILG
                     KARFVYYPLDEIKWIQ"
     gene            complement(624..1166)
                     /locus_tag="IWD16_002127"
     CDS             complement(624..1166)
                     /locus_tag="IWD16_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9089127.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(1403..1801)
                     /locus_tag="IWD16_002128"
     CDS             complement(1403..1801)
                     /locus_tag="IWD16_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9089128.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            2061..2921
                     /gene="ylqF"
                     /locus_tag="IWD16_002129"
     CDS             2061..2921
                     /gene="ylqF"
                     /locus_tag="IWD16_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9089129.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            2914..3681
                     /locus_tag="IWD16_002130"
     CDS             2914..3681
                     /locus_tag="IWD16_002130"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP9089130.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            3737..4594
                     /gene="dprA"
                     /locus_tag="IWD16_002131"
     CDS             3737..4594
                     /gene="dprA"
                     /locus_tag="IWD16_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP9089131.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            4716..6794
                     /gene="topA"
                     /locus_tag="IWD16_002132"
     CDS             4716..6794
                     /gene="topA"
                     /locus_tag="IWD16_002132"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP9089132.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            6763..8139
                     /gene="trmFO"
                     /locus_tag="IWD16_002133"
     CDS             6763..8139
                     /gene="trmFO"
                     /locus_tag="IWD16_002133"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP9089133.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         2849 a         1444 c         1720 g         2170 t
ORIGIN      
        1 acaacgctat attaatcgct tctggcttgt attcaaatac ttacttgttt ctgtattcct
       61 tgcgttcatg ttgcggggat ttttattcat cccggtacca gtggaaggca attcaatgga
      121 aaacgtgttg aagcaaggtg atatggttgt gatggaaaaa ttttcggaga tcagacgatt
      181 tgatattgtt gtttttcaat tagcagatgg cacgatttat atcaaacgag taatcggtct
      241 tccaggagaa aatgtcagct accaaaatga tcaattgaag atcaatggga aagtagttaa
      301 ggaaccttat ctaactaaaa acctgaaatc agatcatgca aatgcatcct atacgacgga
      361 ctttacacta caagagctaa cgggacaatc caagcttccg gaagataatt attttgtact
      421 tggagataat cgaagagttt caaaagatag tcgttcgttt ggcacgatca ataaaactga
      481 tattctaggt aaagcaagat ttgtctatta tccacttgat gaaataaaat ggatccagtg
      541 agaaacaata aagcgtgcag tctgttgaga ctgcacgctt tattgtttaa tccaaaatca
      601 gtttactacc ttagtacgta aaatcaaaca gctcgtttaa acacttttct agcctctaaa
      661 gttttgataa atgaataaac aaccattatt tgaataggga tttggatcat attcctgatg
      721 aatctaccta gaaaaattac tttgaaatct gcaccaaaca aaacagacat cagccaagaa
      781 ttgataaaaa ggctgactgg gatcataatc aataattggg cagcgatgac ggaaggcagg
      841 tttatctttt tatgatataa aaataagcca ttaataaatc caccaaggaa agccgtgata
      901 agaaaagcag cttgaaacgt tcctgaacca aatatcgtga aatttaaagt gttcgacaac
      961 atttcaagca aaccagccca gatcggtcca tatagataac ccataagcgc catacttaaa
     1021 aatccgaatg tgatttgaaa ataagcagga ccaatcgcta tcttgtataa gatgacatca
     1081 agggctaata aaagagcaga ggtcgacaac aatcttgcat tcatttcggg caatctaaaa
     1141 acgtcttttg caatcaaagt gttcaaaata ttctctcctt tgtcagttaa taataaaagg
     1201 ataccataaa atcaccaatg tgtcatgaca cattggtgat ttacatacac tggcatctaa
     1261 caattaaaaa aagggtgaga agactgcttt tataaactgt aaaaaagaga ttgtacgaat
     1321 aaaaaaaata gaggcggaga atacttatcg tatcatccgc ctttgttttt ttgttataaa
     1381 aaacgtcaga ttagttatct gcttacatct tcatccatac gggttctaat gtatttccgt
     1441 ctagatcttg gacttctaat gaaaacattt cttcttcagg cattcccata tctacttcgt
     1501 ggaaagtacc accattttct tttgcaattt gagcaaattt ttttacttca tccgcgtttt
     1561 caacactaaa cgaaatcaaa gcaccacttg tttttttatt atcagctatt tctttatctt
     1621 ttataaattt tttataaaaa tcatgagaga gaagcattat ccaaaaagtg tcatcccata
     1681 ccattgaacc agcttcttca gtagaaaatt cttcattttt tttaaaaccc agcttttcat
     1741 aaaaagcaac ggaacgattg acatcagcaa caggaaaatt aacaaaaacc atactattca
     1801 tttgtttcgt ctcctttctg ttttgttcgt ctatcatctc ttatttagga aacgctgtca
     1861 aaaatttaga ttcatatata gaaaccaaaa taaaaatttg catcatcaaa aaagtgaaga
     1921 tgtgatagaa tgaggactgg ttcactttca cttaactaat agatggaatt tctttataat
     1981 aaaaaagtga atttatattg aaaaagaaaa aaacaagtaa agccggggaa tattccggta
     2041 tgaaaggaat ggagagaaca atgaccatcc aatggtttcc gggacatatg gcaaaagccc
     2101 gacgagaagt ttctgaaaaa atgaaatatg tggacatcgt atttgaattg gtagacgcac
     2161 gattaccttt atcttctcgt aatccaatgc tagatcagat cattcagcaa aaacctcgcc
     2221 ttgttttatt gaataaagcg gatctggcag atccacaaca gacaaagctg tggcaagaat
     2281 attttcgtaa acaaggccat cttgcattag ctatcacggc acaagaaagt aaaggaatta
     2341 aaggtctagt acctgcggct aaagaagcat tgaaagaaaa acgggcaaga gatgctgctc
     2401 gggggatcaa gcctcgcgct attcgggcaa tggtcttagg tattccaaat gttgggaaat
     2461 ctacgttgat gaatcgacta gtaggcaaga aaattgcaca gacaggaaat aagcctggcg
     2521 taacaaaagg tcagcagtgg cttcgtttag gtaatgagtt ggaattgtta gacacaccag
     2581 ggattttatg gcccaaattt gaagatgaag aaatcggcaa aaaactagcg ctgacaggcg
     2641 cgatcaaaga ccagcttgtc catttagatg atttagcgat ttacggatta gacttttttg
     2701 cccgatatta ttcaggaaga atcagcgatc gttatggttt atcaatcgag caagaagaaa
     2761 aattggcacc ggaattactg atggatatta cagcaaaacg tgggtataaa gaagactatg
     2821 atcgtggcag cgaattagtc gtgcatgata ttcgtcaagg aaaacttgga cgatacacac
     2881 tggatcgttg cgaagaaatg gagcaaaaaa gtgatgactg aatccatcca aagtatcaaa
     2941 gaacgattga aaagcataca gagaatagat gatcctacac tagaatcatt aagaaatgat
     3001 ccacgcaaag gcgtgcagca agcactggct tcttttgaac gacgattgaa aaaggaagca
     3061 ttaattaagg aaaaatatga agaaatgttt tgctttgaaa agcaagggta cgcacaaggt
     3121 caccaatgga ttgccggcat tgatgaagta ggaagaggac cattagctgg acctgtcgtg
     3181 gcagctgcag ttatcttgcc gaaaaatgag atcatattag ggctgaatga ctccaaacaa
     3241 ttatcagaga aaaagcgaga acttctttat caacaagttc aagaaaaagc cctggcaatc
     3301 gggatcggga tcgttgatca gcagacaatc gatgatgtga atatttatga agcaagtaaa
     3361 ttagcgatga tccaagcagt agaaaaattg tccattgtac caagctattt gttgatcgat
     3421 gcgatgacct tggatttgcc tattcagcag gaaaaaataa tcaaagggga tgcacgatct
     3481 gtgtcgattg ctgctgcaag tatcattgct aaagtatatc gagaccaatt gatgaaagaa
     3541 tatgacgtgc tttatcctgg atatggtttc tcgcataatg caggttatgg aacaaaggaa
     3601 cacttagaag gacttgaacg acaaggaatt acgccaatcc atcgtaaaag ttttgcacct
     3661 gtaaaaaaat atttacaata aaaaacagcc gcttttacgt atcttattag aaagagagac
     3721 gaaaggcggt tttatgatgt atcaaataga agaaaattta ttgaaacttg cttatacaaa
     3781 aggaatcagt agtcaaggaa aatgggcact tgctaactgg atgatgcagt atccttataa
     3841 agaaatcgga tttgaagaca tcattaagat aggtaaaata accaaacatc gggagctgtt
     3901 tcgcaaaagt tggcaggaaa ttcacgctaa ctggggcaaa atccaatcga aacagtcgtt
     3961 tatcacttgc tttgatccca aatatccccc tcagctgctt cacttgacct atccgccaat
     4021 tgtactgttt tataatggtg atctatcttt actttcttgt aacatgttaa gcgttgttgg
     4081 tggaagacac acttctgctt tagccaaaaa aacagtttat tatttactaa aaccagtgat
     4141 agaagctgga tatgtgatcg tcagtggctg cgcgaaaggg atagatacat acggtcacca
     4201 agcagcaatc tctcatggtg gacggacaat cgcagtcatt ggaacaggga tagagcaagt
     4261 ctatcctgct gaaaatcgtg acttgcaaga ggtgattgca aatgatcatc ttcttttgag
     4321 tgaatatcct cctcaatccg gcccgaaaaa gcattgtttt ccaatgagaa atcggattat
     4381 cgcagctttg tctaaaggaa catgtgtctt tgaagccaag aaaaaaagcg gctctctgat
     4441 aacagcacag caagcattgg atcttggaag aacagttttt tctgttccag gaaatatttt
     4501 aacagaacat tctaccggat gtcatcattt gatccaagac ggtgcagttt gcacggtttc
     4561 gggtgaagat atcttggcag aatttaaaga ataacaccat acttttcatt tgcttttctt
     4621 gacaaacgtt ttctgaatgg atatgattag ctacgatttg tatgaatcct cttttaatag
     4681 taggaacacg acaatggaaa ggagtccatt cagtaatggc gtataaatat ttagtgattg
     4741 tagaatcacc tgcaaaagcc aagacaattg agaaatatct tggacgaaac tataaagtaa
     4801 tggccagtgt tggccatata agagatttac cgaaaagtaa aatgggtatc gattttgaaa
     4861 acaattatga accccattat atttctatac gcggaaaagg cgatgtcatc aaaagcctga
     4921 aagccgcagc aaaaaaagct caaaaagttt acttggcaag tgacccggat agagaaggag
     4981 aagcgattgc ttggcattta gcgtacctac ttgggttgga tctgaaagaa aaaaatcggg
     5041 tggtcttcaa tgaaatcaca aaagacgcag tcaaagcagc ttttaaggaa ccaagaacga
     5101 tcgatgtaga tttagtagat gcacagcaag ctcgtcgtac cttagacaga atcgttggtt
     5161 attcgatcag tcctattctc tggcgtaagg tcaagaaagg gttaagtgca ggacgtgtcc
     5221 aatctgtcgc tttaaaaatt attattgacc gtgaaaaaga gatccgagaa tttgttccag
     5281 aagaatattg gagcatcgac ggtaatttta aaaaagctcg caagaaattc aaagcaaatt
     5341 tctggggaat cgacggtaag aaaaagaaat taccagatgc acaaagtgta aaagaagtca
     5401 ctgctagaat tgatggaaca gacttcgaag ttaaaaaagt agaaaagaaa gagcgcaaaa
     5461 gaaaccctgc gaatcctttc actacaagta gcctgcaaca agaagctgcc cgtaagttaa
     5521 actttcgaac aagaaaaacc atgatggtag cacagcaatt atatgaagga atcactttgg
     5581 gtaaacaagg aacagtcggc ttgattacat acatgcgtac tgattctacc cggattgccg
     5641 atacagcaaa agcagaggcc gcagcattta ttgaagatac gtatggaaaa gaattttcta
     5701 gacatgatca ccgaaagatc aaaaatgctc aaggcgcaca agatgcccat gaagctgtgc
     5761 gtccaacgag tgtgcttcgt acaccagatg aactcaaaca atatttagat aaagatcaat
     5821 tgaaacttta tacattgatc tggtcacgtt ttgtggctag tcagatgaca ccagctattt
     5881 tagatacaat gaaagtgaca ttagaacaaa atggtgtcat cttcattgcg aacggttcaa
     5941 aaatcaaatt caaaggattc atgcaagtgt atgtggaagg tagagatgac ggcaaagaag
     6001 agaaagaaaa tattcttcca gaacttgaag aaggcgatgt agtccaatct gttgatattg
     6061 aaccaaaaca acattttact caaccaccag cacgttttag tgaggctacg ttgattcgtg
     6121 cactagaaga aaacggggta gggcgacctt ccacttatgc accaacactt gatacgatcc
     6181 aaagacgcta ctatgtaaaa ttgacacaaa aaagatttga gccgactgaa ctaggagaaa
     6241 ttgtcaattc attaatctgt gaattcttcc ctcaaatcgt cgatatccat ttcacagcag
     6301 aaatggaagg agatttggat aaaattgaag aaggcacgga agcgtgggtc aaagtggttg
     6361 atcgtttcta taaaccgttt gaaaaagaac tgacgaatgc ggaagaaaaa atcgaaaaaa
     6421 ttcaaatcaa agatgaacca gcaggattcg attgtgatgt ctgtggacat ccgatggtga
     6481 taaaattagg gaaatatggg aagttttatg cttgcagtaa ttttcctgat tgtcggaata
     6541 cgaaaccaat cgtaaaagaa atcggtgtga cttgcccagt atgtcatgaa ggacaagtca
     6601 ttgaacgaaa atctaagaaa aacagactat tctatggttg ttcccgtttc ccagaatgtg
     6661 aattcacttc ttgggataaa ccagtgggca gaaattgtcc aaaatgtgac ggatatctgg
     6721 tcgagaagaa agtcaaaggc ggtaaacaag tagtctgtgt aaatggagat tacgaagaag
     6781 acatccaaaa ataatagaaa agaggttctt tcattgaaaa tggtaaatgt tgttggagca
     6841 gggcttgccg gaagtgaagc tgcttggcag atcgcacaag caggagtccc tgtccgttta
     6901 tatgaaatgc gtccagtcaa atcgactgag gcgcatcaca caagtaattt tgctgaacta
     6961 gtatgttcca attcattaag aggaaacagt ttggctaatg cagtagggtt attgaaagaa
     7021 gaaatgcgcc gattgaattc ggtagtaatc acttcggcag atgaaacagc agtgccagct
     7081 ggtggtgcct tagctgttga ccgcgattct ttttctgaaa caattacccg aaaagtcaaa
     7141 gagcatccat tagtaacagt gataaacgaa gaaattactg aaataccgga aggaatcacc
     7201 gtagtagcaa caggaccttt gacttctcat ccattagcag aaagcatcaa agcattcaat
     7261 ggttcagatg gtttttattt ctatgatgca gctgcaccaa tcgtagacaa agctacgatc
     7321 gatatggata aagtatattt gaagtcccgc tatgacaaag gagaagcagc ttacttgaac
     7381 tgtccgatga cagaagagga attcaaagct ttccatgagg cactaatcaa tgccgaagtg
     7441 gcagaactta aatcttttga gaaagaaaaa tattttgaag gatgtatgcc aattgaagta
     7501 atggcacagc gaggattcaa aacgatgctt ttcggaccta tgaaaccagt tggattggaa
     7561 gatcctaaaa ctggcaaacg tccttatgct gttatccagc tgcgtcaaga caatgctgca
     7621 gcttctttat acaacatcgt gggctttcag actcacttga aatggggcga gcaaaaacgt
     7681 gttttccgga tgattcctgg gttagaaaat gctgaattcg ttcgctatgg tgtgatgcac
     7741 cgaaacagct ttatgaattc gccagaattg ctgaaaccga cttaccaatc aacgaaacgc
     7801 gaagatctgt tctttgcagg tcagatgact ggtgtagaag gctatgttga aagtgcagcg
     7861 agcggactac ttgctggaat caacgccgca cgcttagcaa aagaaatgga accagtcgag
     7921 tttccacaag aaacagctat tggaagtatg gcttactaca ttactcatgc ggaaggaaaa
     7981 catttccaac cgatgaatgc caactatggt ctgtttcctg aactgccaga gcgcattcga
     8041 gacaaaaaag caagatatga agcaattgca caacgagctt taaaagctaa tgaggcaata
     8101 aaaacagaat tagaaaaaac tgcaatccca aatgagtaag gaaactccat gaagagggtg
     8161 agacggatag tctcgctctt ttt
//
LOCUS       DACDMM010000122         8139 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDMM010000122 DACDMM010000000
VERSION     DACDMM010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.123"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..728
                     /locus_tag="IWD16_002134"
     CDS             <1..728
                     /locus_tag="IWD16_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089134.1"
                     /translation="KLLSLMTFIGLIKKLDDEDVPLKMLEYLNRLKEEKALGNFNHKR
                     SNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEWVKICFNEKEAKRVFPQNS
                     RSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVCERLRNVGDNSVKNALIKN
                     RGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGIDETIKELILSGAGIVPLY
                     QFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(804..1139)
                     /locus_tag="IWD16_002135"
     CDS             complement(804..1139)
                     /locus_tag="IWD16_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9089135.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            1266..1481
                     /locus_tag="IWD16_002136"
     CDS             1266..1481
                     /locus_tag="IWD16_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9089136.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            1518..1880
                     /locus_tag="IWD16_002137"
     CDS             1518..1880
                     /locus_tag="IWD16_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP9089137.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            1926..2252
                     /locus_tag="IWD16_002138"
     CDS             1926..2252
                     /locus_tag="IWD16_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089138.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            3095..4918
                     /locus_tag="IWD16_002139"
     CDS             3095..4918
                     /locus_tag="IWD16_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9089139.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            4932..6341
                     /locus_tag="IWD16_002140"
     CDS             4932..6341
                     /locus_tag="IWD16_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9089140.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            6359..7195
                     /locus_tag="IWD16_002141"
     CDS             6359..7195
                     /locus_tag="IWD16_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9089141.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            7260..8042
                     /locus_tag="IWD16_002142"
     CDS             7260..8042
                     /locus_tag="IWD16_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089142.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
BASE COUNT         2658 a         1266 c         1729 g         2486 t
ORIGIN      
        1 ataagttact atcacttatg acttttatag gattgataaa aaaattagat gatgaagatg
       61 ttcctttgaa gatgctcgaa tacctaaata ggttaaagga agagaaagcg ttaggaaact
      121 ttaatcataa gagaagcaat gtctatcgat tagacgtttt taatgcaact tctgtgattc
      181 aaaaattgaa tgaagatatc cttccacttc tggaaaaaat tcattttaca tatcaatatt
      241 tttcttatga atgggttaag atatgtttta acgaaaaaga ggctaaaaga gtatttcctc
      301 aaaattcccg atcagaactc agcaaggaaa aacaattgat tttagatgaa gcacatgaag
      361 ttttacaggg cttatcgtgg caagggacat atctaatgac agagaaagaa cttattaaca
      421 ctgtttgtga acgcctacgt aatgttgggg ataacagcgt gaaaaatgct ttaataaaaa
      481 acagaggcta ctttgttaaa caaggattca ctcttgaacg agcttctaag gatattaaga
      541 aactatacaa tgtgaaagca agtagtagtt ttttaatata tttagcgaaa ggggcaggga
      601 tagatgaaac aataaaagaa ttgatattat caggagctgg gatagttcct ctgtatcaat
      661 ttacaagtgt aacaagagat gatggacgga gagtaaggaa gagaaaagac ccaattgtat
      721 taaactagac aggttatcca tagctcttaa tcttatttca tctcaataca cccaccgtta
      781 aagttcggtg ggttatttat ttttcaatct ttcaatagct gattgcgttc attttcttgc
      841 ctttttaata gcttcatata gtcaattgtt cgaactgcat cttcagtgct aagagaacga
      901 tagagagcca aaaactcttc ctcataatct acgacaacat tgtctagaaa atggcttaca
      961 ggggttccta gggctctaga aagagctgtt aagctatcga ggttaggctc tgttacagaa
     1021 ttttcccatc ttgctactgc ctgttgtgta gagaagattt tttcagctaa ttctttttgt
     1081 gtccacccga gagcttttcg cttttctcta atgacctttg catattgttt ctgtttcatt
     1141 aatacacccc catctcagta tagtacaaca agttgttgta tttgtaatgg tatttgaagt
     1201 tttttatatt ttatgagttg aaatacatca tattgttgta tataataaaa caggagggga
     1261 gaatcatgaa aaaagtaagt aatctcagcc gatacagaaa agaaaaagga ctttctcaaa
     1321 cggagttagc aaaaaaaatg aatgttacac agcaatgtgt aagttcgtgg caaacaggga
     1381 gaacgattcc taaaccctat caaatgaaga tgctttctga gatattaagt gtaccaatga
     1441 atgagctgtt ttctgatgta tttaataaag taaacagtta actaataagc aattgaatga
     1501 ttttaggagg agaagacatg tattgtagaa aatgtggcaa agaaataaca gatgaaagta
     1561 agtattgcca gttttgtgga gcactacaag aaacagatga gacaaccgga acagacacag
     1621 taaagaaaag tactgagcag acaattaatg aaatgaagga acaaatcgta catggacaag
     1681 agacagggaa gctgtatttg attattggtt ggatttctat ggtagtatcg ttagcattta
     1741 ttcctgttgt atttggtgct gttgctgtta ttatgggcta cttatatcga gaaaaagatg
     1801 agaaaatggg tacgattttg atgatagcag gggtagctgg agcaatcttt ggtgtacttt
     1861 tagggatgtc aatgtactga taagagggat aagagcaaat tattttggag ataggagata
     1921 aaaagttgaa taaagtaaat atgaacaaga agtttgtcat tataggtatt gttgtcttgg
     1981 ctattatagg tattattttt tcaaaaatga caggtacatc tggattagag aagtcatcaa
     2041 aaccattggt aacacagatt ttacaagaac agtacaactt aaatcgatca tgtgatgatg
     2101 tgacgattac tcatgaaaat ggagataaca cataccgggc taaagctata ttagacaatg
     2161 gaagtgctat caacataaat attgagtact atccaaagaa agaccgtata tatgtggaga
     2221 taccatatgc tgaggtgtta atgttaaatt gatttaaatg tattatgcat accgtccaaa
     2281 caggaactcc caaaggtgta aattcactag ggagttttta tagtttccta taaattaaat
     2341 actaatacag gtgacaaaaa aatattacaa tgattgtcgg gaatttttat gttattcact
     2401 gttataacaa tataaaagaa atgaaaaaga aaaaattatt atggatatgt ttggtatttt
     2461 tattgataat tggtggaatt gaaatactta ctacatgtag attagaaata aatcagttta
     2521 aacatttgct agttaatttt taagaatact agcccaaatc attctatcga tacctttata
     2581 tctcggaatg attgaaattg gctattgctt actgattttt accaaaaaaa caagtatcac
     2641 tattgttgcc tatattgttg gaatgctgtt tattccatcc tttactcacc aaattcatca
     2701 atttttccca agagcagaat tgttgaagtt gtgtgatccg ttatatgcat ttttgatgct
     2761 ttcacgattt tgggaatttc ccgctggtta tgtaggaatg gttttactgg tatagttaat
     2821 attagatata cttattttag cagtaagtat acgcagatat accctagttg atgtgatcta
     2881 actgttatat taccctatac ggtcaaacga aaagataaaa aattaaattc cctgttgatt
     2941 gcgctatcat aatgtgctat tattggatta cagcaaaata acgttattgg catgttactg
     3001 attatgcagg caagaccaag acaaactaac acttcaatgt tgattggggc gtggtttgac
     3061 ttggtctttt ttactgcttt aaggagagag aataatggat tacagagaag taggaagaaa
     3121 tgtattgcaa caggttgggg gaaaagaaaa tgtggtcagc ttaacccatt gtgccaccag
     3181 actgcgtttt gaactcaaag ataaaaggaa agccgatacg aaagccttag aaaaaacact
     3241 tggtgtaatt agtgtggtgg atagcggtgg acaataccaa gtggtgattg gcaatgaagt
     3301 ccaaacagcc ttcaaggaaa ttcaaaagga agtgggaact tcaattaaaa agggcccctc
     3361 ttcagacaat caagaaagta ttatttcaag attgattagt gtcatttcta ccacctttac
     3421 accgatgatt ccagctataa caggtgccgg gatggtaaaa gccattttgg ccgtcttgac
     3481 tttgactggt gtactatcag acagtagtca aacttacatc attttgaata ctattgctga
     3541 tgcagccttt tattttatgc cagtactatt agcctatggg gcagccataa agtttgatgt
     3601 caacccaatt ttggcgatta ctgttgctgg cattttgctt catccaaata tttcacagct
     3661 tttttctact ggcgatgccg tttcttttct aggaattccg gtattagctg cagattatgc
     3721 gggatcagtt ttaccgatta tctttactgt ttggatcatg tcctatgtag agccctttgc
     3781 ggatcgaatt tcgccttcgt ttattaaatt ctttactaag ccgatgctgg tatttttaat
     3841 tatggggcct ttagcgttgg ttgtcattgg accgtttgga acgttgctga acgatcttat
     3901 agcaatgggg gctgaatgga ttaacggcaa agcagcttgg ttaattccat ttttgatggg
     3961 tggtttgcag ccatttttgg tggttacggg tacagcttgg gcaatgacgc caatcgctac
     4021 tatgcaattg agcagtacgg gttctgaaat gatcaatggg ccaggaatgc tggcttctaa
     4081 tattgctcaa tcaggtgcta cctttgctgt agccgcgaaa actaaaaaca aagagttgaa
     4141 gcagctagct gtgtccactg ggatcactgc tttaatggga attactgagc cttcactcta
     4201 tggagtaacc ttgaagctga aaaaaccatt agttgcttcc atgattggtg gtgcaattgc
     4261 tggtttgtat gctggcttga gcggtttagt cagatatgcc tttgtttccc ctggcttagc
     4321 agccttacca gcctttattg gagaaaatcc aatgaatatt gttcacgcaa ttattacgtg
     4381 tgtaatcgcc tttgttgcaa cctttatcat tgcttatcta attggatttg acgatacttc
     4441 tgatgaatca gagcaaacgg caatagccga agcagtcatt gttgaaaatc cggtaaaagg
     4501 aaccgtgtgt cctatttctg aagtgaacga tgctgtcttt tctaagggac tattgggaaa
     4561 gggcgtagcc attcatccag aagaaaacag catttattca cctgttgagg gagaagtcat
     4621 tactatttta gaatctaaac atgcgattgg tctacgttct aaagaaggta ttgaattatt
     4681 gatccatgtg gggatcgaca ccgtgaattt aaaaggggaa ccctttgaag tatttgtaaa
     4741 agcaggagat caggtgtcag caggacaatt attaatcaac gccgatttcg ctgccattag
     4801 aaacgctggc tacgatgata cagtgattgt ggtagtgacc aacagtgaaa attacgcagg
     4861 aattagtgaa gcaagcggaa gcttaaagga acacgaacca ttgatgcaaa ttagttaagg
     4921 agaggatttt tgtgcaatac aaacagcttt cagcatttcc agagaagttt ttatggggtg
     4981 cctccattag tgcctaccag gcagagggtg cctatcagga ggatgggaaa caaccgtcta
     5041 ttattgacaa gtatcagcat acagaagggg tagctaattt tgaagttgcc tcggattttt
     5101 accatcacta taaagaagat atacagctgt ttaaagaatt aggcttaaaa gcttttcgct
     5161 tttccatcgc ttggacgcga attatggata ttgaaaataa taaaaccaat caaaagggaa
     5221 tttcttttta tcatcaagta attgatgaat gcctgaatca gggaatcgaa ccgattgtca
     5281 ccatgtacca tttcgattta ccttattttc tagaggagca gggcggttgg ttaaatcgag
     5341 caactatcga ttattttgtg gactatgtac aggtattgct tactgagtat ggtgacaagg
     5401 tgaactattg gctgacaatt aatgagcaaa acacgatgat ccttcatcct ggagcgattg
     5461 gtttgccacc aagtggagag ctgccttcaa aaaaagaact ctttcaaatt aatcaccatg
     5521 ttttattagc tcaagctaga gtaattcact tgtatcatca gttgaatctt aaaggaaaaa
     5581 tcggtccagc aatcaattta acggctatgt atcaagctac ctccagtcca gaagatgcaa
     5641 ttgcagccca taattgggaa acattgagag gctggtcttt cttagatgta gcagttcggg
     5701 gaaaatacaa ttatttgttt gaaaactatt tgaatgatcg tggattatat cctaaaattg
     5761 aggcagagga tcaggcaatt ttatcctccg gtaaacccga ttttattgcg attaattatt
     5821 attcaactgc caccatcgct gccagcaaaa atgacggatc agatgtttcc gctagagcgg
     5881 gggatcaaca gatcatgttg ggagaagaag gtgtctaccg ggcagctgaa aatccttatg
     5941 tagataaaac gccatatggc tgggtagttg accctactgg actacgatta acattgagga
     6001 aattgtacga tcgctatgat ttacctattt taattactga aaatggctat ggggcacccg
     6061 acatagttga ggaagataga cgaattaatg accaggatcg aattgattat ctagccaaac
     6121 atattctggc tattcaagca gctttaacag atggtgtaga tgtttttggt tatttacctt
     6181 ggtcagcgat tgatgttgtt agtacccatc aaggcttcaa taagcgttat ggttttatat
     6241 atgttgaccg aaccgatgag aatcttaaag aattaaaacg aataaaaaaa gaaagcttct
     6301 tttggtatca aaaagtaatc gcaagaaatg gcttagctta gagagaacgg tgaatctaat
     6361 gagagtaatt aaaacgttaa acaacagttt aattctttgc ttagataagc aaggcaaaga
     6421 agtaattttg atggggaaag ggattggatt tcaactaaag aaaggcgatt tggttccggc
     6481 tgacaaaatc caaaaagtat ttaaattaga ttcactttct gatgctctaa aaaaggatta
     6541 cattaaactg tttgaggctg ctccggatga tctaattatt tgcgtcaagc gaattatcga
     6601 ttatgccaac gccaagtttg actttaagct gaataaaagt ctatttttta ccttggttga
     6661 tcacttgaag catgccattg aacgcagtca aaaaggcatt gtttttcaaa atcgcctgct
     6721 gattgaaatt caaaagtatt acccaaaaga atttgagatc ggctgctatg ctatccaatt
     6781 gatcaacaaa aaaatgaatg ttcagttgcc agaagaggag gctggaaaca ttgcgtttca
     6841 cattgtaaat gcccaaagtg aacaagcaaa tatggaagaa accatgctct cggtaagagc
     6901 actaaaggat atcttggcta tgctaggata tctgtttccc gaaaaaaagg ttgataaaga
     6961 ttcgatgctt tacttaagat ttgtgacgca tttacagttt tttattgccc gaatgattaa
     7021 gaaggaagag ttgccggaga aggatgattt tttgctggct tcagtgaagc agcgttttcc
     7081 gaaagagtat cgggcggcct tgaaaatcaa ggagtacgtt caagaagaga tgggtgcctc
     7141 agcaaatcag gacgaactgc tttatctaac actgcatatt tcaagggtgt actgaatgaa
     7201 gaaaatatcg gctgcacaat atataaagac ttgttggttg tgctaaggct cctgattcct
     7261 tggaaaattc ttctaacagt gcggaggtga atgtttcgac ctctaccagt gaagtggtaa
     7321 agtgttcaca agaaactact agcttgccag aagagctact tggagattgg cagattaata
     7381 ataaggatgc tggtatagag atatcagaca gtaaagctgt attccgtata ggaaggtctc
     7441 aagtagagtc aaaggattat tatgtagatg taaacaacaa aatgctttta catgtctatt
     7501 tttcttttga gtatgatagt gtgaaataca ttgaagtctc tattgaaaga actggttata
     7561 gtgaatttat tagtaaaagc gtgggaattt taactatgca cactaaagat ggacaatata
     7621 taagtttggg tgtgaagaga gtttccggtg agaataatga gacagaagca gttacggagc
     7681 agacgatgga tgaaagaatc gattcatcta agagtaatga gacagaagta gatactggga
     7741 agacgataga tgaaagaatt gattcattca tcgaaacata cacagctata ccacctactt
     7801 ggaagcttgt aaatgaagat aaaggaatca cgataaatac tgatacagag atttctacag
     7861 atgctcaatc tttatcagat ttattagcta atctaacagg tatcagtaat gcagtgacag
     7921 agcaagttgg taaagacatt ccacttcggt tacttgaaaa tgggaaaaaa gattattctg
     7981 tggtatttaa gaacggacag gtaactgatt acaacgaagt atttaaaaaa gttattgaat
     8041 aatgattctt ccatcttttt catttattta ccctaccccg ttgttatatg accgtactta
     8101 tacatctcca gtagcttagg ataagctact gggggtatt
//
LOCUS       DACDMM010000123         7980 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDMM010000123 DACDMM010000000
VERSION     DACDMM010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.124"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            119..820
                     /locus_tag="IWD16_002143"
     CDS             119..820
                     /locus_tag="IWD16_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9089143.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1429..2445)
                     /gene="tsaD"
                     /locus_tag="IWD16_002144"
     CDS             complement(1429..2445)
                     /gene="tsaD"
                     /locus_tag="IWD16_002144"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP9089144.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2442..2906)
                     /gene="rimI"
                     /locus_tag="IWD16_002145"
     CDS             complement(2442..2906)
                     /gene="rimI"
                     /locus_tag="IWD16_002145"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9089145.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2913..3455)
                     /gene="rimI"
                     /locus_tag="IWD16_002146"
     CDS             complement(2913..3455)
                     /gene="rimI"
                     /locus_tag="IWD16_002146"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9089146.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3439..4263)
                     /gene="tsaB"
                     /locus_tag="IWD16_002147"
     CDS             complement(3439..4263)
                     /gene="tsaB"
                     /locus_tag="IWD16_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP9089147.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4352..5332)
                     /locus_tag="IWD16_002148"
     CDS             complement(4352..5332)
                     /locus_tag="IWD16_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9089148.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5356..6840)
                     /gene="galT"
                     /locus_tag="IWD16_002149"
     CDS             complement(5356..6840)
                     /gene="galT"
                     /locus_tag="IWD16_002149"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP9089149.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6852..7841)
                     /gene="galE"
                     /locus_tag="IWD16_002150"
     CDS             complement(6852..7841)
                     /gene="galE"
                     /locus_tag="IWD16_002150"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP9089150.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2254 a         1704 c         1407 g         2615 t
ORIGIN      
        1 aacaaattat atttgtaaag tattaaaact tgtttctaat gttggtatgt taaaacttta
       61 caaattcttt aaaaaaagtg acaataaata tagtgaacaa taaggagagg actttttaat
      121 gcgtgttaat aaaatcgatt ttcacttttc aaaactcaga tatcaacctg attttgaaca
      181 tttctcggat gaagagttcc aacagatcaa ggaacatact gttcttcgtt cttataaaaa
      241 aggacagatc cttttcgacg atggtgatcc cagacaacgg ttgtatatct tgaccaaagg
      301 actcgttcgt ttagaacgtt atgatgaaag tgcaacttat tactactatg actatgtgaa
      361 tacaaataac ctttttccta tgtcaggaat gtttggagaa gagacctaca cttatacagc
      421 tcaagcgatg actgatattg aaacattcta ccttcctgtt tcctactatg aaaaattagc
      481 acgaaaaaat cccgaacagt tgatctatat gatacaaaaa atttctaaag tagttgaaat
      541 acatgaattg cgtatccaaa tcggattgac ctctagcgcc tttgaccggg taaaacaaaa
      601 tttattcatt ttgaaagaag aattgggcgt accaacaaaa gatggtcata tcaccattcc
      661 ttatccgatc accttaaaag aattagcagt gaatagcggc acgacacgtg aaactgctgg
      721 acaagtaatc aaacaactac gtgattccgg gctgttagat tatgacaaaa agcgttttac
      781 tttctgtaag acagctacta ctccagctat gagagaataa cgcgaacaaa aataagcagt
      841 gaccctgata aatatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg
      901 gcagttttag atcactggac aagactcaaa gattttctat gtgtgtttca gttatgtaat
      961 attcactgtt tgcaaaaaat actgttagat taactagatg aagcgtcttg tcctcatccg
     1021 atctagatta ttcgatattc ctcgacaata aacgattact cagtgagggc aatcagagga
     1081 acggataatg ataaataagc tgtaaaaaat agcaaaataa caataatgat taaaagtatg
     1141 taaaaaatag ggctttgaca aaaacgtgtc acagccctat ttcgattaaa cggtgtgtac
     1201 ctgtcaactc ttcggtcaca agtcataatg ttcataaaca tgaggagctg ttttttgaaa
     1261 ttgttccttg tgaatcagag ttaaacgaca ggcttacgaa ctcttgatta aacggcgttc
     1321 aaaagcttgc atacgcggta ataagaacag ccaggtcaaa aacatgagaa gccgtttttc
     1381 ctgggctgtt cttattactt gatgcagggc agctttttca caacctcttt attcagataa
     1441 aaccaagctt ggatcagcat tcaactgata gctagcaaaa tttctttttt gcatttccac
     1501 atgtgctgct gctccaatca ttgcagcatt atctccacaa agtcgaagag ggggaatcac
     1561 tagttctacc tcagggagtt tagctgccaa agcatcctgc aatccttcac gcaacccttg
     1621 attggccgct acacctccag ctataatcag ttgcttgact ggataatttt gacaagctcg
     1681 aagtgtttta gtaatcaata catcgatcac acttgcttga aagctagctg ctaaatcatt
     1741 tttgtctaga tcttctccac gctgttgtgc attatgcacc aaattgataa atgcactttt
     1801 taatccgcta aaactgaaat cataattatc ttcatggatc atcgctcgcg gaaaatggta
     1861 attatctttt ccctgatgag ctagctgatc gatttctttt ccgcttgggt aagaaagacc
     1921 taatactctt ccaactttgt cataagcttc tcctgctgcg tcatctcttg tctccccaat
     1981 gatttcatat gatccatctt cttgcatata gactaattct gtatgtcctc cgctaacaag
     2041 aagtgccatc aatggaaact ggaatggttt aaccagacgg gctgcataga tatgccctgc
     2101 catatggttt acaggaataa gcggcaattg gtgtgcccaa gcaaaagcct tagctgcaga
     2161 aataccaatc agcaacgagc cgacaagtcc tggtccataa gtgacagcga ctgccgataa
     2221 atcttctgcc gacacaccag cttctaccag tgcatcttcc aagcataagg tgatttgttc
     2281 tacgtgatgg cggctagcta cttcaggtac tacgccacca aaacgtttat gactgtttat
     2341 ttgagaagca acgatgttag ataagatctc tgttccattt ctaacgaccg ctacactcgt
     2401 ttcatcacaa cttgattcaa tcgccagaat caattcttca ttcatttcat cgtctccgtt
     2461 tttaactttc tgctcataat gatcgcatct tccgtcggat tgcgataata tgatttccgt
     2521 ttacctaata tacgaaactt ttctgattca taaagttttc ttgctgcttc attagataga
     2581 cgtacttcta aaaaaacatg cagcgaatct tttatttgtt ccgtaatcag taattgtaac
     2641 aaatgccgtg catgcccttt tccttgttct gaaactgcaa cagcaatatt tgtgatctcg
     2701 acttcatcta ttactttcga gtatccaata aagccacaga tctggttatc ttcagctaaa
     2761 agcagataat cagagtgagg ctgttggatg tctgccaaaa actgcatttt cgtccaaggt
     2821 gaaccatgtg gataagcatt ttcgctaatc atccatacgc attctgccaa gtacgcttca
     2881 tcaaaatctt tttttctgca aatcaactaa tctcactcat tttctaaaag ttttatcata
     2941 tccaacgcat cttcttttgt ttcagtgtaa tatctttttt tgatcgttgt tgcttgaaac
     3001 cccaatttcc gataaagtct ttgtgcatct tgattactca tacgcacttc taatgacatc
     3061 gtctcacagc gattcattat agcaaagttc tcgatttcgt caataagcag cgaaccgatt
     3121 cgtttacgct gatatgccgg tcgaacggca atattcgtga tatggcaatc taaaccgaac
     3181 gctcttgaac cgataaaacc aatcattctt ccattatcaa agatacctag atatagatga
     3241 ggaagcggtg acttcaattc tgatataaag gcactttttg tccatggcaa ttcgccatta
     3301 tagacttctc gttcgacatc cagcagctct ttgatatctt ctgtagtcaa ttctcttagt
     3361 acgtaacgac gatcgatttg ttcgactgtt ttttctgtat aaatcatttt atcttgaaaa
     3421 aagcttctgg catacgctct aaattttttc aacataactt tcgtctccag gtgtgtgcgt
     3481 ctccaaccat ttttcttctg cttctactcg ttttaaatag cgaggaagaa aagcttgttt
     3541 ctctgcaact ggttcaacta aagcacctaa ttgagccaca gttacaccac ttggaatatc
     3601 ccatgctgga actgtattaa tcatcgcact aggcaatatt tcctttattt cagagaagaa
     3661 tttgtgacaa tcactcccga cgaaataaat agcttcattc agcttttgta gttgtgtgaa
     3721 caaatcagaa gcagaaatat gtacatcttc aagtacagtt tctaatctat tttctttcca
     3781 gcggtatgcc ccagcgtata cattttttct tcgagcatca aacatgggca caataatatt
     3841 atccacaccg atgcaattag ccgcgatggt tttcagacta gatactccta ctagctcttt
     3901 atctaatgta taagcaatcg tttttgctgt cgttacacct atacgaagcc ctgtataaga
     3961 accgggacca tcagaaaccg cgattcggtc aatgtctttt ggacttattt gaagatctgc
     4021 aaacaattgg tcgattgcag gcatcagtgt tacactgtgg ttgcgtttga tatttgtttg
     4081 tatttgtcca agaatttgtt cgtcttctac cacaccgatc gccaaagttt ggtttgcagt
     4141 atctattcct aatgtaatca tatatttgat cctttctgct atgacattct cgccttccat
     4201 tgtagcatac ttttttattc tgaggatgaa aataccaaag gcaaaactag tacaataata
     4261 catgattatc tgctggcggg caaattttgc cccttcctga aacacaaaaa agctgcggta
     4321 tcactccatt ttcccacagc ttttccatct tttatttcgt tgattctcga ataatcaact
     4381 ctgtttccac aataatcttt cttgaaaccg gtgctttgga attacttaat tgaagaatcg
     4441 tctccactgc tagctctccc atccattcgg tataaacttt tacagtagaa agcgagggcg
     4501 ttacatattt cgctacactg acatcattga agcccatcac agaaatatct tctggtacca
     4561 ctaaaccttt ttcttgaatc gctttcagtg cgccaatcgc taatgcatca ttagctgcaa
     4621 aaagcgcatc aggaacatgc ggattcgttt ttaaatattg cttcactgct tgatatcctg
     4681 actcgacaga aaaatctgct tcgataattg gaccttcgtc gtagcctttt tctgccaaaa
     4741 cttgttgaaa tgccataatt ctgggatctt ttagccgctg atggtttttc ttcgtatatt
     4801 ctgttccagc aagcatagcg atatgttgcg ctttatggga gacaaagtgt tcaatgactt
     4861 tttgaacact tgacttaaag tcgacaacta gagagtcgat tccttgagaa gtgccgtcag
     4921 aatctaccaa aagcaaggca ttttgggatc ctttcatttc taataactga gcttcgtcga
     4981 attttcctag tgcgatcgtt ccatctgccg gaagatcact aatggaatcc cacgtttctt
     5041 tcaataaagt aacgtttgct tcttctgcct ttttttcgat tcctaggcga atagataaat
     5101 aataaagatc ttcaagctct tccgtatcgt cgtaccactg gatcaggcga agcgtttgct
     5161 tagtatttgc tgcattttta cgatgtttcg tataatttaa gcgttctgct gcttcaaaaa
     5221 ttttttgctt tgtggctaag ccaacggaaa gatcaggatc atagttgaga actcttgaaa
     5281 cagtagcagg agaaactcct gccaactgcg caatgtcttt aattgttgcc atttgtctct
     5341 cctccttttt tacgtttata aaatcgcagt aaatcgttca aacgctgcct gtcctttttc
     5401 atctcgcttg aatactccag cgtcttccag tacacgtgca aaaacctgac ctacagattt
     5461 ttgtacgaca tcggtgaccg tatctttcgt caaatcagga tgttcttttt ttaattgttc
     5521 tgcccacgga cgatgatatt cagccatctg gttttcttct cctaataaga aatgctctac
     5581 ttctactagt tctggtttca aacgaggtgg taaaacagca agccccatta cttcgatcaa
     5641 cccaatgttc tcctttttga tatgttggac atctttatgt ggatgaaaaa tcccatcagg
     5701 atgttcttct gaaacattgt tgtctcgaag gacaaggtcc aactcaaagt aattgtccct
     5761 tcttcgtgca attggcgtaa tcgtatggtg tggtgtacca tctgctgaaa aagctctgat
     5821 agatactgtt tcatccgaat atgctttcca tttttccaat actgagttag ctgcttcaat
     5881 aagcaagttt ttctctgttg attgcaaacg gatcacagac atcggccatt tcacgactcc
     5941 tgcgatgact tgcggataat cttctagttt gataagtttt tctatcggtg cttttgccat
     6001 aggaaattca tgacgtcctg cctgataatg atcatgagaa agaatcgatc ctccaacaat
     6061 tggtaagtca gcattcgaac caacaaagta atgaggcaag acttcagtaa tccgaagcag
     6121 tctagcaaat gtttcctttg tgattttcat cgggcggtgt tgttctgata aaatgatcga
     6181 atgttcattg taataagcat aaggggaata ttggaatccc cagctttcac cgtctagatt
     6241 cattcgaata atccggtgat tggtgcgggc tggatagttc aagcgacctc tatatccttc
     6301 gttttccatg caaagcatgc aggtaggata gtctactttt accgcctcgc gttctgctgc
     6361 gatttgtttt ggatcttttt ctggcttaga taaattgatt gtgatttcca gttcaccata
     6421 ctccgactct gccggaaatt tgatgttttt tgcgatggct cttgttttga tgtagtcatt
     6481 tttcttactt aaattaaaga aatactcagt tgcttcctct gggcttttcg aatagtgctg
     6541 tgcaaagaat gcattgacga ctgaaggtgg cggtgtgaga aaatccatta attgcgcttc
     6601 aaaaatctct tgttctgctg gcaattctga aagaacacca ttttctcttg ccttagcgac
     6661 aagctgatcc aataagtcta cagattcttc cttaactggt cttacttcaa tgctttctag
     6721 agcatcttct cctaccatcc ctaaaagacg attctgtaaa tataagcggt ccatttccat
     6781 ccatccgcca gcttcgatcg caagtgtggc aaaatcacaa atcgtttgac tgattgacat
     6841 atctcattcc cctattcttc ataaccattt ggatggctga catgccaatc ccatgctgtt
     6901 ttgataatcg ctttgatatc tgtgtattca ggtttccagc ccagaatctc tctcgctttt
     6961 tcacttgaag cgactaatcg gctaggatct ccagctcttc ttggtgctat ttctgctgga
     7021 atttcttttc ctgtcacttc tctagctgct tctagcattt ccttgactga gtatcctttg
     7081 ctgctgccta aattgaagaa gttgctttca tttccttctt tcaagtattc caaagctaaa
     7141 atatgtgcag caatcaaatc ttctacttga acataatctc tgatacatgt tccatctggt
     7201 gtatcataat cgtctccata aacagccaag gcttttcttt gaccaagagc tacttgtaaa
     7261 atgattggga caagatgcgt ttctggtgta tgatcttctc cgatagatgc atctgctttt
     7321 gctccggcta cattgaagta gcgcaaagcg acatagcgca tgccatatgc ttggtcacac
     7381 cacttcatca ttttttccat catcaattta ctttcaccat acggattttt aggattagct
     7441 ggtgtatctt ctgtgattgg agactctttc ggctctccat aagttgctgc agtagaagag
     7501 aatacgattt tattcacgtt gaactcgtgc atgacttcaa gtaagatttg cataccgtat
     7561 acgttgttgt tgaaatacat caatggtttt tcgacagatt ctcctactaa agagcttgca
     7621 gcaaaatgga ttactccttc aatcggttcc ttttcaaata cggaacgtag aaattcttta
     7681 tcccggatgt ctccttcata aaaatgagca tctggatgta ctgcttgttt gtgacctgtg
     7741 agcaaattat ctaccacagc tacttgatac cccttttgaa ctagctggtc tacagcgtgc
     7801 gaaccgatat aaccggctcc gcctagtact aaaattgtca taaaaaacac tcctttgatt
     7861 agtaaaattg tgtttgttga ttctatataa tttaaaaaac gaagtttact tttatggtga
     7921 aaacaaacaa agtaaaattt cgtaaattca tatggtaaat ttataataaa acatttagta
//
LOCUS       DACDMM010000124         7976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDMM010000124 DACDMM010000000
VERSION     DACDMM010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.125"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(21..995)
                     /locus_tag="IWD16_002151"
     CDS             complement(21..995)
                     /locus_tag="IWD16_002151"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9089151.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="IWD16_002152"
     CDS             complement(1075..1950)
                     /locus_tag="IWD16_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP9089152.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="IWD16_002153"
     CDS             2080..3987
                     /locus_tag="IWD16_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9089153.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="IWD16_002154"
     CDS             complement(4528..7806)
                     /locus_tag="IWD16_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP9089154.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2273 a         1667 c         1474 g         2562 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat
     7921 ataacttaag ttgttgataa taaagagatt tattaacgta aaaagaataa gttttt
//
LOCUS       DACDMM010000125         7886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDMM010000125 DACDMM010000000
VERSION     DACDMM010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.126"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            301..1029
                     /locus_tag="IWD16_002155"
     CDS             301..1029
                     /locus_tag="IWD16_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9089155.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            1047..2243
                     /locus_tag="IWD16_002156"
     CDS             1047..2243
                     /locus_tag="IWD16_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9089156.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            2236..3231
                     /gene="lacD"
                     /locus_tag="IWD16_002157"
     CDS             2236..3231
                     /gene="lacD"
                     /locus_tag="IWD16_002157"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9089157.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            3228..4169
                     /locus_tag="IWD16_002158"
     CDS             3228..4169
                     /locus_tag="IWD16_002158"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAP9089158.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            4345..4824
                     /locus_tag="IWD16_002159"
                     /pseudo
     CDS             4345..4824
                     /locus_tag="IWD16_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            4837..5727
                     /locus_tag="IWD16_002160"
     CDS             4837..5727
                     /locus_tag="IWD16_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9089159.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            5714..6523
                     /locus_tag="IWD16_002161"
     CDS             5714..6523
                     /locus_tag="IWD16_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9089160.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            6537..6938
                     /locus_tag="IWD16_002162"
     CDS             6537..6938
                     /locus_tag="IWD16_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9089161.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(6984..7886)
                     /gene="yidC"
                     /locus_tag="IWD16_002163"
     CDS             complement(6984..7886)
                     /gene="yidC"
                     /locus_tag="IWD16_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9089162.1"
                     /translation="MLFLSGCVRMDSNGNPDTSGIVYRVLVHPMGQAITYLVENFNWS
                     YGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVEAAQQKLKAATTREEQM
                     AAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARYTEGIRESSFYGIDLGS
                     PSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAMIVFMSISAPAGVTLYW
                     VVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPRKDVTESAKPANNKQAT
                     KQIKQPKNSTNNKGRNAGKQQKRK"
BASE COUNT         2518 a         1288 c         1729 g         2351 t
ORIGIN      
        1 ttagttgttg taaatgatat tggaagtaat aaaacgatag ttgtaggaat atcataggga
       61 ttaatgtgga tcacgctgct cgataagtta gaccattttg acggatcgct tatgtggttc
      121 gtcttttttt ctgttctttt attggtaact ggtatttttg aggtatcttt gtctattcaa
      181 gatataaagc tttaaagagc tgttataata gcttttttgg tctaaaaaac ctttgacaaa
      241 tgattaataa ttcactattc ttgtgttgtg gttataacca gtaaagaaga ggtgattttt
      301 atggagagct caaataaagg aaaatcattg tattatcaac tagttgattt attgaaggat
      361 cgtatcgaaa cacagatgac tccgcatgat aagctgttat ctgaaagaga actttgtcaa
      421 cactatgggg tgagtcgtac caccgtacgt ctggctttac aggaattgga aaatcaaggt
      481 tatatttacc gaagacatgg aaaaggtacc tatgtctctg atttgtctga taagtcatcg
      541 aacttagctg gagcatatag ctttacagaa gaaatgcgcg aaatagggcg cgtccctaag
      601 acggtcattt tagaatttag acaaatggaa gctaataaga gaattgctaa gaatttgaac
      661 atccatttag gtgaaactgt atttaaactt aaaagattgc gtttggcaga tgaagagcca
      721 ttgatggttg agcgttctta tctaccggcg aaactattta tgggacttga tttgccgtta
      781 cttgaaagta aaccgttgta tgatgtgttt tcacaggatt atgaacaaca agttcaatta
      841 gctgatgaag agttttatgc aagtatcgtg caaaaaaaag atgctgttgt gctacgaatc
      901 gctgatggcg ctcccgtctt acatctgacg aggataacct ataacgtgaa aaatgagatt
      961 atcgaatata cagatagtgt agcaagagcg gaccagtttc attataaaat tcggcatatc
     1021 cgaaaatgat tgagagggag aacaacatgt ttgagctatc gaagaacaaa ttaaaacaaa
     1081 taggtgcagc aatcacgaca caagaaataa aacaacaacc agatctctgg aaagaaacaa
     1141 tcgatagatt gatgcaaagc caagaaaatt tagctctatt ttttgatcaa ataaaaaaag
     1201 aagcaaacgg aagaagaata cgtgttattt ttacgggtgc aggaacgtct caatatattg
     1261 gggatacgct cttaccctat ctaaatgaac atggagacaa tcagtttttt atcttccaca
     1321 gttttggcac gacagatatt gtagcttcac caaaagaatt tttgtttcca aatgaaccga
     1381 ctttattagt ttcatttgct cgcagtggca acagtccaga aagtgtagcg gctgtgcaaa
     1441 ttgcaaatga acaagttgaa acattgtttc atttgacgat tacttgtgct aaagagggga
     1501 tgttggcaaa aaacgcacag gatgataaac agaattatct gttattgatg ccagagagag
     1561 caaacgatgc aggttttgct atgacaggaa gttttacgtg tatgttattg agtgctttat
     1621 ggatctttga tcaaacagca atggagaaaa aagaaagata tcttgaacaa ttagttaaaa
     1681 tcggtcaaga tcttctttca agagatcaag aaattcaaaa gttattacct gatttttttg
     1741 aacggatcgc ttatctgggt tcaggaagtt tggcaggtgc gacaagagaa gcacaattaa
     1801 aaattttaga attaacagca ggtaaagttg ctacaatttt tgattcttcg atggggtttc
     1861 gtcatggacc aaagtctttt ttaaatagcg agacactcgt tgtaggtttt gtgagcaatc
     1921 aaccatatac gcgaaattat gacttagata ttttagagga aataaaagaa gatcgggtca
     1981 ctgccaatgt cattgcgatt gcgcaaccgg gtactattga tttctcaggt atgactgtgc
     2041 tattggaaga ctcggtgagt aaattgcccg atgcctatct tgcattagcg atgattatga
     2101 ctgcacaaat cattgcactc tatacttcaa tcaaagtagc taataaacca gatacaccat
     2161 ctccaacagg cacggtcaat cgagtagtca agggcgtcat tattcataag tatgaaaccg
     2221 aaggagaaat gacagatgaa tgaagaaaaa aatagggcaa tggagagact atcaacaaaa
     2281 gatgggatta ttagtgcatt agcgatcgat cagcgtggtg ctctaaaaaa aatgatcaaa
     2341 tcgttgggaa aagagccaag tgaagaccaa atcagtgaat tcaaagaact agtatcagct
     2401 gagctgacac cctatgcttc tgctatttta ttggatccgg aatatggatt gccagcagca
     2461 aaagtcagag caaaagagac ggggttattg ttagcttatg agaaaaccgg ctatgacgca
     2521 actacgcctg gtcgtctacc tgatttatta gcggattggt cttctctcag attgaaagaa
     2581 cagcaagcgg atgcagtaaa attcttactt tactacgatg ttgatgaagc acccgaaatc
     2641 aatcatttga agcatgtgtt catcgaacgt ttaggtagtg agtgcgaagg agaaaatcgt
     2701 cccttctatt tggagttagt ttcttatgat gcacaaataa gcgatagttc ttctccagag
     2761 tatgcgaaag tcaagcccca caaagttatt gagatgatga aagaattttc tgatccaaaa
     2821 tataaagttg atgtgttgaa agtagaagta ccagtcaata tgaattatgt gaatggctat
     2881 tccacaggtg aatttattca cagcaaagaa gaggctgctg ccttctttaa ggagcaaagt
     2941 gaagcgactc cattaccatt catttttttg agtgggggtg tttcaacaga attatttcaa
     3001 caaacactga tttttgctca tgaatcgggt tccaccttca atggtgtatt gtgtggacgt
     3061 gcgacatgga aagatggggt caaagcatac gtcgaaaatg gaaaagacgg agctattcat
     3121 tggttaagaa caaatgggag agaaaacatc gagactttaa ataaagtaat aaatgaaacc
     3181 gctacccctt ggttcaataa aacaaaaagc aaaaaggtga agatgaaatg attacgacag
     3241 ttaccttgaa tccttccatc gatatcgcgt atacaattga agaattaaac ctcgacaaag
     3301 tcaatcgagt tgaaacagtc aataaaacag cgggaggaaa aggagtgaat gtggcgaggg
     3361 ttctaaattt attaggagaa gaggtgatag ctactggttt aataggtggc cagcacggtg
     3421 aatatctaaa gaaccagcta gatcagttag ggatcatgca tcagtttgtt ccaattaaag
     3481 gagagacaag ggattctatt gctatcctgc atagttcgag acaaacagag cttttggaac
     3541 gaggcccctt cataacaaag aaagaacaac agacatttac cgatcatttc gagcagctcc
     3601 tcgaaatatc agatattatg acgatctcag gtagtttacc ccaaggatgc cctcttgatt
     3661 actataagca attggttgct ttagctgata aaaaagggaa agacatatta ctagatactt
     3721 ctggtaaatt tttgcttgaa gtattggaag gtaaggagaa gccactactt atcaagccca
     3781 atctggaaga attaggagaa ctgattggcc aacgtatctg ttcttcgatt caattagaaa
     3841 aaattcagtc cttattagct catccaatat ttgaaggagt gccatgggtt gttgtttctt
     3901 taggagagag aggtgctgtg gcaaaagttg ctcagtgttt ttacgaagtc attattccta
     3961 aaatatcagt tgttaatcct gttggttcag gtgacgcgac ccttgcagga tcggcccatg
     4021 cgttatcaaa aaggcaatca atcgaagaga ttctcgcatt ttctatggct tgtggtatgg
     4081 caaacgcgca acaagagaaa actggctgga tcgaccctat gaaagttacg atgttgatga
     4141 aacacattca agttaagaaa atcaaatgaa cttaaatata aaagaaagcg ttttctttag
     4201 ttgggaagga atttgttctt agcttaaaaa tgaaatataa gaaagaaaaa atgaaaacgt
     4261 tatctttgaa acagaaggga tatttgatga tgtcacagtt ttaattgaac aataaataga
     4321 aaaaatagat aaggagaatg catgatggca attattggtg taagaatcga tggacgtttg
     4381 atacatggat aagtagcaaa tctttggaca acgaaactga atattagtcg aatcatggtc
     4441 gtggatgatg aggtagccac aaacacaatt gaaaaaagtg gattgaagtt agcgactcca
     4501 gcaggggtaa aattaagcat tttgccaatc gaaaaagcag caacaaatat tttagctggc
     4561 aaatatgact ctcaacgagt attgatcgta gcaaaaaagc cagatcgaat cttatcttta
     4621 gtagaaaaag gagttcctat caaagaagta aatgtgggga atatgtcaca aacgaatgaa
     4681 acacggtcca ttacaaaatc aatcaatgtg gtagatgcag atatcgaagt atttaaaaaa
     4741 ttagatcagc ttggtataca tttaattgct caaatggtac caaacgataa agcagaagac
     4801 tttatgaatc ttttaatcaa ataggaggaa tgatccatgg atattcaatg gtggcagatc
     4861 ttattattaa cattatatgc aggttatcaa attttagatg aattacagat ctattcttca
     4921 atgagtgcgc cagtgtttgc agggttatat gcaggtttag taatgggtga tttaaacgca
     4981 ggtttgatta ttggtggcag tatgcagttg acagttttag gagtggggac gtttggaggc
     5041 gcatcaaaaa tagatgccaa ctctgggacg attttagcaa ctgccttctc gatttctctt
     5101 ggaatgaatc cagaacaagc gattgctgct attgcagtgc ctgtagcaag cttaatgatc
     5161 caattggata ttttagctag attcgctaac acatactttg cccatcgtat tgaccgtcac
     5221 gtagaagaca tgaattatca agcaatcgaa cgcaatttct taatgggagc tttaccttgg
     5281 tcgctctctc gtatgattcc tgtattttta gctttagcat ttggtggtgg attagttgaa
     5341 aatgttgttt ctgttttaaa cgggaatcta aaatggctag gcgatggact ttcagtagca
     5401 ggagctgttt taccagctgt tgggtttgcc attcttctgc gctatttacc agtaaaaaaa
     5461 catctatctt atcttatttt aggatttacg atcacggcat tattagcaac gttattctct
     5521 aatgttcaat tattaggaac aagtgtggca agcgttgtta aagatcttcc ggcaactttc
     5581 aattcgctgc cgatgcttgc tgtagcattg attggtctag cactagcaat gatcagttat
     5641 aagaatgatc aaaacaaaga ccatcaacca ttagctagtg agccatcaca ttctgttgag
     5701 ggggaaatcg aagatgacga aatctaatta caaattaaca aaagaagatt ttaagcaaat
     5761 caatcgaaga agtttgttca cttttcaatt aggttggaac tatgagagaa tgcagggttc
     5821 tggctattta tatactatct tgccacaact aagaaaaata tatggagaca atacaccaga
     5881 attaaaagag atgatgaaaa cacatacgca attttttaat acttctaatt tttttaatac
     5941 aatcattact gggattgatc ttgcaatgga agaaaaagag ggaattgctt caaaggaaac
     6001 cgtatcgggg ctgaaagctg gattgatggg ttcctttgca gcgattggag attccgtatt
     6061 tgctgctttg attccaacga tttttggggc tttggcggcg aatatggcaa ctgatggaaa
     6121 tccgataggt gtgtttatat ggatttttgc tcagatcgtt gtcatggttt ttcgttggaa
     6181 gcaacttgaa tttgcataca aagaaggaat ttccttagtt actactatgc aatctcgctt
     6241 aactgcatta acggacgcag ccactttatt aggggtattc atggtagggg cattagttgc
     6301 gacaatgatc aatgttaaat tttcatgggc accaacgatt ggcgatgtga cattgaatat
     6361 gcaaaactat atcgatatga tcttacctaa aattttacct gctgggatcg tcggtggtgt
     6421 ttattggtta ttaggaaaga aaaacatgac tcctactcga gcgattttta tcgtattaat
     6481 tgtgtgtgtt gctttatctg cattgggtgt gatttcgaaa taagggagag cgaaaaatga
     6541 gtagacaatt ggtattgatt agtcacggaa aattttgtga ggaattgaaa aaaagtacag
     6601 aaatgatcat ggggcctcaa gaggatatct acacgttacc tttattacca gaagatggca
     6661 ctgaaacata caaacaaaag tttctcactg tcacagattc gttaaatgat tttgtggtac
     6721 tatgcgatct tttaggtggt acacctgcaa atatcatttc taaactaata atggaaggac
     6781 aacagattac tctttatgct gggatgaatc ttccgatggt cattgaattt atcaactcac
     6841 aaatgattgg ttcagagccc gagtttgttc gtgcaggatg tgaaagcatt gtctctgtca
     6901 acgaattaat taatggaata gcagaagagg aagagtaaca aaaaagatga gggcttagct
     6961 ctcatctttt ttgtcctatg tttttatttc cgcttctgct gctttcctgc gttgcggcct
     7021 ttgttgtttg tactgttttt tggttgtttg atttgttttg ttgcttgttt gttgttagct
     7081 ggctttgctg attctgtcac gtcttttctt ggagtgacga cttgttttgg cgggttttgt
     7141 ttcaactctt ctgccacttg tgctttcaaa cgtggtttca tcataacgtt tgtgatgaat
     7201 gtttgcagac agctgaacac accaccgact acccagtaaa gtgtaacacc ggctggcgca
     7261 ctgatcgaca tgaagacgat catagcagga gagacgatta gcatcgtacg catcgtcttt
     7321 ttctgttctt caggaatacc gatcgttgag atgtatcctt gaagcagata ggcgacccct
     7381 gcaatgacaa caagtaccat acttggtgag cctaaatcga tgccgtagaa agatgattcc
     7441 cgaatacctt ctgtataacg tgccgtaaag tacaaggcag agaagatcgg catttggatc
     7501 aatagaggca gacagccgat tccgccggtc atactcaatc cgttttcgcg gtatacttgc
     7561 tgcatttctg cttgtgctgc catttgttct tcacgtgttg ttgcagcttt caatttttgt
     7621 tgggctgctt caacttgtgg ttttaacgct tgcatttttt cagattggat cattgttttt
     7681 ttcgattgac taatacctag tggcaagatg atgatacgga cgatcacagt cattgcaata
     7741 actgcccagc cataagacca attgaaattc tcaactagat atgtaatcgc ttggcccata
     7801 ggatggacga gtactcgata aacgattccc gaagtgtcag ggtttccgtt tgaatccatc
     7861 cgcacacagc cagataaaaa gagcag
//
LOCUS       DACDMM010000126         7806 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDMM010000126 DACDMM010000000
VERSION     DACDMM010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.127"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            141..1907
                     /locus_tag="IWD16_002164"
     CDS             141..1907
                     /locus_tag="IWD16_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP9089163.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2009..3268
                     /locus_tag="IWD16_002165"
     CDS             2009..3268
                     /locus_tag="IWD16_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9089164.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3415..4716
                     /locus_tag="IWD16_002166"
     CDS             3415..4716
                     /locus_tag="IWD16_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9089165.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4719..5570
                     /locus_tag="IWD16_002167"
     CDS             4719..5570
                     /locus_tag="IWD16_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9089166.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5583..6398
                     /locus_tag="IWD16_002168"
     CDS             5583..6398
                     /locus_tag="IWD16_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP9089167.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6549..7715
                     /locus_tag="IWD16_002169"
     CDS             6549..7715
                     /locus_tag="IWD16_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9089168.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         2534 a         1355 c         1571 g         2346 t
ORIGIN      
        1 aagtctatct atttggcaag tttaaaaaat aaaaattcgg tatcaaaaaa ctgttaccga
       61 taacattttt gaaagcgttg tcttttgttt ctctatcact tatagtgatg acgtcatatt
      121 aaaacacaag gaggaaatca atggatacag ctgcaattta tcatcgaccg gaaagtgaat
      181 atgcatttct atatacgaat acaagatttc gtattcggct tcgtactagg aaaaatgata
      241 tcaaaaaagt atatgtgctt tgtggagatc catataccat aacaactgaa aaatggtatc
      301 aaaaacaacg accaatgaaa aaatgtctaa gtaccaatgt tcatgattat tgggaaattg
      361 aagtgacgag cgaaacaagg cgcttacagt atgcgttcca tgttgttggg gaagataaca
      421 cggactgttt ttacggtgat caaggaattt ttccttatcg agaaaatgtg attactgaac
      481 caaattttta ttttcgaata ccttattttc atcaaatcga tcgattcact attccagaat
      541 gggtaaaaga aacagtatgg tatcaaattt ttcccgaaag atttgcaaac ggggacaaag
      601 caaatgaccc cgaacacact ttaccttggg gaagtaaaga tccagaccga gaagacttct
      661 tcgggggaga tttgcaaggt gtgagggatc atttggatta cttgacggac cttggcgtca
      721 atggcatcta tttttgtccc atatttaaag ccacatccaa tcataaatac gatacgattg
      781 attattatga ggtggatccc gcttttggcg ataaaaaact tttaaaaaac ttgattgatg
      841 aagcacataa aagagggata cgtatcatgt tcgatgctgt attcaatcat atgggtgtcc
      901 attcaccaca gtggcaagac gttctcaaaa acggagaaaa atcgatctat aaagactggt
      961 tccacatccg ttttttccca gtagattctt atcagatgac tgacttacca gaaactgctg
     1021 aaaatatccc atatgatact tttgctttta ctccattcat gccgaaacta aatacagcaa
     1081 atccagaagt gcaaaaatat ttgctggata tcgcaactta ttggataaaa gaattcgata
     1141 ttgatggatg gcgtcttgat gttgctaatg aagtcgatca tcatttttgg aaaaaattca
     1201 gagaagcagt tacagaaatc aaaccagaca tctatatcct tggagagatt tggcattcct
     1261 ctcaagcttg gctgcaaggg gatgaattcc atgcagtgat gaattatgca ttcacagatt
     1321 cgattaaaga ttattttgca aaaaagaaaa tcacagcttc acaaatggtc agtggaatga
     1381 atcatcagca aatgctttac agagatcagg tgaacgaagg aacattcaac ttattagatt
     1441 ctcatgatac tgccaggatc ttgacccttt gccaaggtaa caaagaattg atgaagtctg
     1501 tgatggcctt tatgtttctt caaaaagggt ctccttgtat ctattacggg acagaaatag
     1561 ggatgactgg tgaagatgac ccggattgcc gcaaatgtat gatatgggag aaggaggaac
     1621 aagatctaga gctattcggt tttataaaag aacttgtttc tctaaggaaa cagttgtcta
     1681 agattatttc tgaaggttct actcagtggc taatagtata tgatcgtgaa gataagctct
     1741 atttcacacg ggaactagag ggacagatca tctatgttta ctttaatcag tcaaaagaac
     1801 cttgggtagt agaacaggaa aatgaggtga ttcttagtca aaattgtcag ttgttagaag
     1861 acgggaaagc agagatacga caatacggat ttttgatcat gtgttgaaaa ctgttaccgg
     1921 taacaacaag aaagcgtttg caaatgaaga tttgtgccca ttataataag ggtgtaacag
     1981 cacgttgaat acacaggagg aaatgaagat gaaaaaaaga agtgtgaaac agttgggttt
     2041 agggttaatc tctttaggaa tcttaggcgg attagcggct tgtggcaatg gtaactcttc
     2101 agcagaaggg gaagccgatg aaggaaaaac gttaacagtt tccgttgatt ctggatataa
     2161 agattatata aacgaaataa aaaacacttt cgaaaaagaa aacgatgtga aaatcaagct
     2221 agtagaaaag gacatgttcg atcagttgga atcattagcg ttagatgggc ctgctggaaa
     2281 gggacctgac gttatgatgg cagcatatga ccggatcggt gcgttaggac agcaaggaca
     2341 tctggcggaa gtaaaactgg gaaatgaagc agcttacgat gaaacagaca aagcccaagt
     2401 gacttatgat ggaaaaatct acggagaacc tgcagtaatc gagacccttg ttttgtacta
     2461 taacaaagat ttagtcgata cagcaccatc aacgtttaaa gatttggaga atctatccaa
     2521 agacagtcgt tttgcttttg agtcagaagc aggaaaaaat acaggatttt tagctaaatg
     2581 gactgacttt tattattcct atggtttaat tgcaggttac ggtggctatg tatttggtga
     2641 tgatggcaca aatccttcag atatcggttt gaataatgca ggtgctgtag aaggaatctc
     2701 ttatgcaaca gattggttca aaaatgtttg gccaaaggga atgcaagaca taaaaagtgc
     2761 aggagacttc gccagccagc aattcatgtc caataaaact gcagcaatca ttgacggtcc
     2821 atggcaagcg caaacataca aagaaaacaa tatcaattat ggtgtagcca aaattcctac
     2881 tctaaataat ggacagcctt atcaaccgtt tggcggagga aaaggatggg tagtcagcaa
     2941 ttatgctaaa aacaaagacc tctctcaaaa atggcttgat tatgtaacga atcaggaaaa
     3001 tcaagaaaaa ttctttgaaa tggtaaatga aatccctgca aaccaacaag ctagggaaac
     3061 agcaaaggct aaaaacgatg aattaacaac agctgtgatt gaacaatatg aaacagctca
     3121 agcgatgcca aacatcccag aaatgggcga agtttggaca ggtgcagaaa acttgatgtt
     3181 cgatgcagca tcaggttcaa aaacaccaaa agaatccgca gatgaagcag taaaaacaat
     3241 cagtgaagcc atagaacaaa aatactgatt aaaatgataa ccgtccattt ttataaaaaa
     3301 tggaacaggt gcttcagata aaagtggcaa gagcgagatg gaaaattatc ttgctcttgt
     3361 tgccattatc ttaaaagcag tttgcggttt agcaagttta gagggggcat tcagatgcag
     3421 caagataaaa caagcaatat caacgaaaaa aaaatcacgc tttgttcact tattccaggg
     3481 atggggcagt ttgtaaatgg ccaagctttt aaaggtatcg tttttttcgc catattcgtc
     3541 ctattcattg gtcagatgct tctgggcgga tttcatgcat tggtaggatt ggttactttg
     3601 ggaagtatac caatggaaga tcattcatta tttattttga ttgaaggcac tttgcaattc
     3661 atcattacaa tcattttctt gattttttac ggtttgaatc ttttagacgc ttatcgagta
     3721 gcgaagatgc gtcaagaggg caaaaaaatc aatcggacga tgaaggaagt catacataat
     3781 ataggtgatg aaggttttcc ttatctattg acacttcccg cttatcttct catgattttt
     3841 acgatcattt tcccagtcct agttacattg ttcactgcat ttactaatta tgacttcaat
     3901 catattccac cagccagttt aattgattgg gtaggggcta aaaacttttt tagtatcttc
     3961 tttttaagct catatcgtaa tacttttttt gcagtctttt cgtggaccgt gatttggact
     4021 ttatgtgcaa caactttgca aatcaccctt ggtattctca cagcggttgt tgctcaccag
     4081 aaatttatta aattcaaacg tattttcggt atcattttct tattaccatg ggcggtccca
     4141 gcttttatta ctatcatgag tttttcgaat atattcaacg atagcgtagg tgccatcaat
     4201 actcaagtga ttcctttcat aaaccagctt ccttttgtag atatcagtgc aatctcttgg
     4261 aaaacggacc cgaactggac gaaagtagca attatcttag tgcagggctg gttaggattt
     4321 ccctatatct atgtgatggt tacaggaatc ttacaatcga tttcagaaga cttatatgaa
     4381 gcagcaaaaa tcgacggagc aaatgcatgg cagcgctttt caaacatcac gcttcctgct
     4441 gtcttcctag tggcagcgcc aacatttatt acacagtata cggggaattt caataatttc
     4501 tccatgatct atctgtttaa caatggtggt cccggaagcg taggcggagg agctggttct
     4561 actgatattc ttatttcctg gatctataaa cttacgactg gaacttctcc gcagtattct
     4621 atggcttcag caatcacgtt gattatctcc gtcatcgtta tctgtgtgtc attgctggta
     4681 tttaaaaaga caaatgcgtt caatatgggg gattaacaat gaaaaaattt cattcgagtg
     4741 ctcgattcca aaaacgactt tctcactttt ttacttactt ttttctttta atactgtcag
     4801 tcattatcat ctatccgctt ttgatcacag caagtactgc cttcaagtca ggaaatattg
     4861 cagcttttag tttagatctt tcaagtgact ggactttagg aaactttact cggctattca
     4921 gtgaaacact ttacgggact tggtataaaa atacattgat catttctgtc ctaacaatga
     4981 tcgtacaagt gactgtagta actttggcgg gctatactta tagtcgctat cgttttatcg
     5041 gccgcaaatc aagtttgatg ttctttttag tgattcagat ggtaccgact atggcagcat
     5101 tgactgcctt ttatgtcatg gctttcttat tagaagcgct cgataagtac tggttcttga
     5161 caatgatcta tataggcggt ggaattccaa tgaatacttg gctgatgaaa ggatactttg
     5221 acactgtacc aatagattta gatgaatctg ctaaacttga tggggcaggg catttcagga
     5281 tttttttcca gattgtatta ccgcttgtca aaccgatgct cgccgttcaa gcattatggg
     5341 cttttatggg accttttggg gatttcatgt tagctaagtt cctgcttcga acacctgaaa
     5401 atcagactgt tgctgtcggc ttacaaacat ttatttctga tgtgacaaac caaagagtct
     5461 ctctttttgc tgctggggca attttgattg cactaccgat ttgtctatta ttcttctttt
     5521 tacaaaagaa ctttgttacc ggcctgacat ctggcggaac aaaaggatag gagcagagta
     5581 tcatgcaaac atttccgtta aactattttt ccgcattacg tacgccaact caagtgtttg
     5641 cgggacgcaa actattatca tggcctaaat ttttcttgat atttgtattt ttagtttcct
     5701 tgatggtcat gcctgtgact cttttttatg ccaatcagat ccaagcgatt ccattagaac
     5761 aatttctatc cgttcattcg ctgatcgatg aacaaggaac ccaaaaattt tctgaacttg
     5821 aattatctga aactgggtta caagcatcgc aacaaacgat agctgtgacc cctgaaattt
     5881 tggtaggtgt gtctttatct gagaaacaac aatccgatca tggaactttt attgattttg
     5941 agaaggaaca atgggtgatc caacaaaagg ataaatctgg tatacgccgc tatacgatga
     6001 attatagccc atctttccaa ccagattctg tacgtacgcc cgaagatttc caacgatttt
     6061 tagaaagaga attttatgcc agtaatcgtc caacaatcat tttatcttat agtttaagtc
     6121 ttggtcttgt cctatttgtc atgacgagtc tcattttatt cggggcttcc tttttcttgt
     6181 ggatgacaag aaaaagccaa ctttcatcca ttcatacatt caaagagagt gcaaatttga
     6241 tgctgaatgt tatgggaata ggttccatgg cagcagcagt tgtagggttt atccactttg
     6301 actttatctt gatgctaagt gtccaaacaa cgataactgt cttgctgctt ttatgggttt
     6361 ttgtcaaaac aagattcaaa gataagcgtg tcgaatgaca agtttaagcg atttatgcga
     6421 caatgaatgt gaaaaaaatg aaaaataaag tagtttttga aaaatttctg aaaaaattaa
     6481 acttcttagt tgattctttt cttttttagt attaacgtaa tagggtagtt tttttaagga
     6541 ggaaaatcat gttagagaaa actttataca atcaattatt ttcacgttct ttctcgctcc
     6601 ctgtcgaagt aaattattgg gacggtacaa cgaaacaata cggagatacg gacactcctc
     6661 cccaaatcaa aatcaatatc catgaagaaa tcccaatgaa agagattaca agtaacgctt
     6721 ctttggcact aggagaagcc tatatggatc atcgaatcga gatcgaggga agtatccaag
     6781 cattgatcaa cgatgcttat cagcaagcaa acagcttttt acgaggaaaa gattatctaa
     6841 aatggctgcc aaagaaagaa aaacatacga aacaaaaaaa caaagaagat atccatgcgc
     6901 attatgactt aggtaacgac ttttataaac tttggctaga tccaactttg acttattctt
     6961 gtgcttattt caatacacct gaagatacgt tggaacaagc acaaatcaat aaagtccatc
     7021 atatcttaga caaactcttt atcaaagaag gcgaaacgtt gcttgatatc ggttgtggct
     7081 ggggaacatt gatgttcact gctaccaaag agtataatgt caaggcaact gggatcacac
     7141 taagtgaaga acaatacgat ttgattacca aaagaataaa agaagaacat ttagaagata
     7201 aatgccgtgt attattaatg gattaccgcg aactaaaagg cgaaacgttt gatcatatca
     7261 caagtgtagg tatgttcgaa catgtaggtt ctgaaaatct tgaaggttat ttcaaagtgg
     7321 taaaagattt attgaaacca aaaggaacag ctttgatcca tggaatcagc cgacaacaag
     7381 gcggtgcgac aaatgcttgg atcaataaat atattttccc tggcggttat gtccctggtg
     7441 ttactgaatt agttgataat attacaaaaa acgatttgca actgattgat ttggaaagct
     7501 tacgtcgtga ctaccaattg acattagaac attggacaaa gaattttcat caagtccaag
     7561 atcaagtcac gaaagaaatg ggtgattcct tctatcgaat gtgggatcta tatttacaag
     7621 cttgtgcagc ttcatttgaa tcaagtaata tcgatgtgat tcaatatttg cttgttcaac
     7681 ctggaaacaa tgacatccca atgcatcgtg cataattgag gcaaaagtac tgtttaaaga
     7741 ctatttaaaa agaagctaag ttttatgatg ctccagaagt gaactttttt gtctaacttc
     7801 taggat
//
LOCUS       DACDMM010000127         7798 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDMM010000127 DACDMM010000000
VERSION     DACDMM010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.128"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            46..2373
                     /locus_tag="IWD16_002170"
     CDS             46..2373
                     /locus_tag="IWD16_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9089169.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            2499..2828
                     /locus_tag="IWD16_002171"
     CDS             2499..2828
                     /locus_tag="IWD16_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9089170.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            2868..3872
                     /locus_tag="IWD16_002172"
     CDS             2868..3872
                     /locus_tag="IWD16_002172"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9089171.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            3869..4315
                     /locus_tag="IWD16_002173"
     CDS             3869..4315
                     /locus_tag="IWD16_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9089172.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            4465..5280
                     /locus_tag="IWD16_002174"
     CDS             4465..5280
                     /locus_tag="IWD16_002174"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9089173.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            5486..6658
                     /locus_tag="IWD16_002175"
     CDS             5486..6658
                     /locus_tag="IWD16_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9089174.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6820..7314)
                     /locus_tag="IWD16_002176"
     CDS             complement(6820..7314)
                     /locus_tag="IWD16_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089175.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
BASE COUNT         2639 a         1459 c         1545 g         2155 t
ORIGIN      
        1 ataacgtgta aaggattatt tgatgatttt ttattgagag gagggatgaa aatgattaag
       61 aaaactcaaa ataaaatcca acgtatatgt caaaaacatt ttgtttctag actcaaagct
      121 gtatctaaaa gaaaatactt acatcccatt atgatttcga tcatcttgat ttttactttc
      181 ttagggagct tatcaagtat ttatgtaaac gtttatacag aaacgtcaaa ttttgctaac
      241 caaacgacag aggagacaaa tagtccaccc tattctgaaa tacagtccat agaaagcacc
      301 aatcagtcta gcaacaacga gacaacctca accacatcct cgactcaact gattgatccg
      361 acagaaaaag aaatcacagc agcaaaagca gcaggggaga aagcttattc agaaacaggt
      421 agaagccaga catttagtac tgttgcggcc actgcgaatt tcaacggaca taccatacca
      481 gatggatata cagtgatata tgccaaagat ggagtcattg ttgcggttaa cggatcgatg
      541 agtgcggata aaactgcgtt acaaggcgta accaaagcaa tcgcttttat tgataaccgt
      601 ccagatatcc ataatctgtt tattggctcc acagtaaatg gtactactat cacattccca
      661 aaaagtatct atatgggggt ggctaccgct ggaaatacga tcgattcagt tacacccaat
      721 gcggataaaa gttattcttt tacaccctca acatggggag ggcttcctgc cggaggaggt
      781 atgagtactt tttcaatcgg tatacagtta gcaaatgata ttccattcac ggggtactcg
      841 atcaaaaatg ggcagatcgt tgatggtaag ggaaatgttt caaattttcc aacctatcaa
      901 gcagcaacgg ttcaggtcaa tgcgaatcgg acaggaactt tttcttatac agtcaatggt
      961 ggagcagcaa aaatcggaac gtacgggact acattatctg gaatccatgt gggtgacaaa
     1021 gtggttatca ctccaacagc agtaacgggc tattcttttg cacaaacaaa cggttctttc
     1081 acagccctag aaagtaacgt cgatacggtg acctatacag gcaaacaaac accaatcact
     1141 attaagtatc aagactattt tggacagatg attgctcctt ctaaaataat gaatcttact
     1201 tacggaagtg ctgcacaaga cctgacgact aatacgccta ctatcaacgg atataccttt
     1261 acagctgttt cagcgataga aacaaagaac caatcagcaa tgagtgtttt agctagttta
     1321 gatacaaatg gaaatgtaat cgtaaaggac gcaaatggaa aacagatttc tgaagtcatc
     1381 ctatattata agacaaatgc aacagtatca atcaatgcaa atggcagtaa gtactatgat
     1441 ggtctttctg ttgtaccgat cgttaaatat gcctttaatg ataaaacaga atcaacatcc
     1501 cttttaaaca atgatctcag tattgatcaa atcacatgga atccaactga tttcaaagcg
     1561 atgaatgaaa agggagttga agtaactgcg cctactgaaa ttggaaccta tacgtcttgg
     1621 caattgacaa aaagcggact agctaaacta gctgccagaa caaactattt atttactatc
     1681 gtgcagacat ctgatgtcta tacgattaag caaatcagcg gtgcggtaga gctcagtggc
     1741 agtaagacat atgatggaaa agcaggtgtc ccgtcaatcc atgtcaagct agcagagggt
     1801 gtcactagtt cgcttactcc agtacaagtg gcacttagca gtatggatta cacagcagat
     1861 gcacaatcag caaaaaatat ggttaatgct ggcagctata caattaaact aacgaatagc
     1921 ggaatagaca aagtcaaagc agcaaatccc aagttgtcat ttactgatat agccaacaca
     1981 tcaggaactt ataccatcta taaagctaac gcagtgatta ctgtcgatga cgctgccttt
     2041 aattatgatg cccaaagtca ctcgatttcg gttggaaatc tccacgttac cggtgttgtc
     2101 ttaggcgaat cattggatta taccctaaca gttaacagcc ggacagatat tggatcacaa
     2161 atagtaacga ttagtttagg cgaaggaagt gtaaacaata attatacgat tatcgcaaca
     2221 gataccgcta agttgacgat caatccgact ctcttgcttc cttcgacagg tgggataggt
     2281 ctactgccct ttgtcttttt aggactgatc tttatttttt ctggcttctt ttattttatc
     2341 catcgtaaga aaaaagcagg tgaacagcga tgaaaaaaac aaaaaatgta cgtgtggata
     2401 tatttgctgt tctctttttt atcctactgg tcttttttat aggttctccg actgtgtcag
     2461 ctagagaatc acctattaca tttactttgc ataatcgaat gttcgaaagc gataagatgc
     2521 caagtatcag tcaaaacgac ggaataaata tcccggataa ctttcaagga gtaaaagggt
     2581 tgaacggcgt tactttcgct atatatgatg tctcggatga attttacaag ctgcggtcag
     2641 aaggaagtag tgtggaagac gcacaaagaa aactagcaca aaaaagtgat agtgaaaaaa
     2701 tactggcaga aaatgtgaca aaaacagtag acgaggaaga gggcattgct tcttttagtg
     2761 catcggacaa agatgagcaa ggtcgtgacg ctgtttaccg atttacggaa ataaagactt
     2821 cagattaagt aaaagaaaaa tcagcaccat ttgtcgttgt gcttcctgtg acagattcat
     2881 ctaatcataa attgacgacc atccacttgt atccaaagag cgaacaaaaa atacctgctg
     2941 cactcacatt aacaaaaaca gtagagaata agcagacaga ttttgcagat ggggataaag
     3001 tcccttatct catcaccgta acaattcctg agaacatcaa cgagatagga acctatacga
     3061 tcaaagatac agctgatcct cagttatggc tagaaccaga aacgatcgat tttttgattg
     3121 gtggagacaa gattcataca tttcacacac aaaaaataaa acatggtttt gtcttatcag
     3181 attctggaaa agatcttagt tcgtttgctg gaaaaaaatt aacgatccgt taccaaatga
     3241 cattgaaaga aaacagtgaa aaaacgacat ttgataacga tgtacgtttg acgactgaca
     3301 atcaaacagt agaaacacat cttgccatcc aaacaggagg caaacaattt gtcaaagtgg
     3361 atcggcaaac gaaacaaaaa ctagcagatg ctcaatttct agtcaaaaaa aatgaccaat
     3421 acttagcgca agaaaacggg ataaatcatt gggtagccaa agatgattca agagcaacga
     3481 tttttacttc cagtaaagat ggaacatttt ctgtccatgg attgtctttt ggaagttacg
     3541 aactagtgga gattaaaccg cctaaaggat atatattgag tcaatcaacc attctctttg
     3601 aagttgaaaa aggcagttat gatccgcttc atcctatacc attggaaatc atcaacgaac
     3661 caaaagcaaa tccgacgaat aatggaaaaa cgccagttca gatgcagaca aatggtccat
     3721 caaatgaaaa aaatggagga agctttccaa aaacaagcga agagatgctt ggaggtttct
     3781 caattttagg gttattgcta gtcctttcaa cagggacagc ttggttttat aaaaaacaac
     3841 aaaaaggaaa caataggagg gaaataaaat gaaaaacatc aaaacaaaaa tatggacgtt
     3901 tttaggaact gctatcatgc tgcttccttt cgttctcggc ctaggaactg cagaggtttc
     3961 tgcagccgta tcacctacac cagaaaatgt tacggttaat ttgcataaat tgaaattcac
     4021 ttcagcacca gaaaatcaaa taaacaacgg aacggaattg acatttccga atagcgaacc
     4081 gttgaacggc gttgaattca acgtatatga catcactgct acgtactatc caagtaaaga
     4141 cacagcagtt ccagcagacg ctacaccctt cgcaagtgta acgacttctg gtgaaggatt
     4201 agctaacctg actcttccag gtaaatctga tgggaaagat gctgtatacg tctttgtaga
     4261 aacaccaaag cccggtgtcg aaacgtcgcc aaatatcgtg ttaagcttac cctagaccct
     4321 gacaaacctt tagacacgat tcacctatat gctaaaaatg tcatcaaaga aagcaatatc
     4381 aaaatccaga aagctgggaa cttttcaggg gtagataagt tagcaggggc gcaattccag
     4441 atcaaaaatt cagagaatca attcgtgacc ggttttgatg aaaacactgg tgtcgctctc
     4501 tatgatcctg ctgagtcagc gttgacattc acaactgata ctgaaggatc catcagtgtc
     4561 aatggattaa tagacggcaa ttatacttta gttgaaacaa aagcaccaga tggttatcaa
     4621 ttaaccaatc cacaaaaaga aactactttt atagtaaaaa acggaaaatt gcaacaaaca
     4681 accggtatca tccatggcgc agatgaagcc aatcttggga atccaaacgg tgtagtcaat
     4741 cgggaaaata caactacgga taacacgatt actgtagaaa atgtccaaaa ctacggtgat
     4801 ttccacttca tcaaacaaga tgtgaacacg caagaaaaat tagccgatgc agaattttct
     4861 attctaagag caaaagatcc aactgttaaa ttaatgaaaa aaatagcaaa ccttgcctca
     4921 ggagaatacc catatgcttg gtcagatgaa gtcacaggtt ctgactggga agaagtgaaa
     4981 ttgatttctg attcagaagg gaaattttca atcgaagggc tgaaacaagg ggcatactgc
     5041 cttcaagaaa caaaagcacc agaaggctac gcattaccta agaatgaagc tgcgtacact
     5101 gaatttacag tagatgatga tccaacaact tctgatgaaa caacgatcga taaccaacct
     5161 aaaggaatct tgccttcaac tggtggtatg gggatcatcg tattcatctt ggtaggtact
     5221 gctttagttg gaggagcagt gatctatttc aaaaaacgcc atcaagaaac agaagcttaa
     5281 actattggaa acaaaaatca tacgataaat aaacgatagt gattcaaatg gggagaaagg
     5341 ttgtgacaag tggtcggtct cttggtattc gctcaacaaa cggcaaaata ggaggaacta
     5401 ttttgccgtt tgcttcgcta atactcaggt caaggcgctg atttcacaac ctcttcatat
     5461 acatattaga aaataggtgg acatgatgaa taaaagcaga ataagaatac tggacatctt
     5521 catggcaatc attttagtcg ttgggatcgg gatcttttct tatccttttg tagaagattc
     5581 actgaatgat tttcttgccc agcaaatgat catccattat caaaagcaag catcgaataa
     5641 gaattcagag gaaataaaaa aacagcagga aaaaatgaca aagaaaaacc aacagttggc
     5701 agagcaaaac gtatcaccag ggataggcag tttcaaccaa gcagttgatg caaaagcatt
     5761 aaaagattta cctagtaatg ccttttttat ggaacatatg cttggagtca tcgaaatccc
     5821 aaaaatcaat gtcagcttac ctatattcga tcagacaacg gaaatatttt tgcaaaaagg
     5881 aacgtcatta ttggaaggaa gttcttatcc gactggcggg aaaaacaccc atgccgttct
     5941 ttctgggcat cgtggactcc cagaagcaaa attatttaca gatttgccta agttgaaaaa
     6001 aggagaccag tttttcatcc agatcaatgg aaagacattg gcgtatcaag tagaaaaaat
     6061 ccaagtggtc ttacctgacg aagtagattc tttgggtatc caaaaaggca gagatctcgt
     6121 gactttgctt acctgcactc cttatatggt caatacacat cgactgcttg tgacggggca
     6181 tcgtattcct tatcaagcaa aagaggcaaa aaaagcgatc caaggaattg atcaatggaa
     6241 aaaatggaaa ttttttattt ggtttatcgg tatactactt ggatcaatcg gcttagtttg
     6301 gctactcatc gcttatttag attctttggc catcgctaag cgaaactatc ctttatcttt
     6361 ttatgtaaag aacacaaacg gacgaccaat cgaaggaatg gtcttttctg taaaaacgct
     6421 caacggcaaa cactatatta ctagagaaaa agttccattt gtgaaagctt ctgatgaata
     6481 cggactagtg agattttctg atttaaaagg caggaactat cgcttgcaac atgaggagct
     6541 cctgcttaaa atccatgtaa aacataaaca ttcgaaacag ttttcaatga aattgaaaaa
     6601 aggaagatat aaactgcgaa aagaaaaaga ggtgtactat ttgattgaga aagaatgaat
     6661 gaagatgtcc aaaaaagcag agggaacaga ctaaatgaaa aaagagagaa gctgacgaaa
     6721 tggacgtgta aactgctatt tttggtttac tatctggaac gccagcttct atttttttat
     6781 gtttcagaag aattttatta ttatagtata acttttgaat tagtttccat aacatttgga
     6841 gcaaggggtt aaaccacgag cttgagcttc gcttaaagtg gcaggggtat acgtaccatt
     6901 tccacattta tgtgtatggt attttgaacc ggtcggtgtg acatagacag tttgttcatt
     6961 attttgctca ggagctgcgg cttgtgcctg ctgagaagcc tgttcagctg ctgcttgctg
     7021 tcgcgcctgt tctgcttgtt gctgtgcgat tgcttcttgc ctcgcctgtt ctgcttgttg
     7081 ctgtgcgatt gcttcttgtt gcgcttgttc tgcttgttgt cgtgcaattt cttcttgctt
     7141 tttccgttct tcttctgctt cttttgcttt gattgtggaa tcaattgcag caagacggct
     7201 tgtcaactct tgtttgtttt caggtagcgc ttggatagct gctgaagcag ttgcatagtt
     7261 ttcttttgta ggatttgctt cagcttgtga caaagcagat tccgcttctg tcactttttt
     7321 ttctagtgca gctttttccg ctttttgttt ctcttcttct tttttctttt caatttgttc
     7381 tttcgatgaa tctgccgtac tttcggagat cgtggtactg ctgctggaag cctgagctga
     7441 acttgtgctt tgagtagtag acgtagtagc accacaccca ctcaacaaac ttcctgcgac
     7501 caaaaacaac gcaactaatt ttttcactaa atttcctcct taagtaaaaa acaaccgttt
     7561 tcaataaatg tccttttcaa ttgtttactt acttgttata ataattgtgt tatgaatagt
     7621 gattgaggaa acttaatcta atcccgtgtt gatacacggg attttttgct atagttctgt
     7681 tattaagtat gcaataataa aatatgtacg ttattttatt attagattat tgacatttat
     7741 ataataaacg attaaatgat tcatatcaat agaatactta ggaaaaaaag agaaataa
//
LOCUS       DACDMM010000128         7684 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDMM010000128 DACDMM010000000
VERSION     DACDMM010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.129"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..216
                     /locus_tag="IWD16_002177"
     CDS             <1..216
                     /locus_tag="IWD16_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089176.1"
                     /translation="IQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLE
                     GTIGYILLDSLYKGTYQSLENLSQKII"
     gene            219..1376
                     /locus_tag="IWD16_002178"
     CDS             219..1376
                     /locus_tag="IWD16_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089177.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1445..2785)
                     /locus_tag="IWD16_002179"
     CDS             complement(1445..2785)
                     /locus_tag="IWD16_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9089178.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(2944..3384)
                     /locus_tag="IWD16_002180"
     CDS             complement(2944..3384)
                     /locus_tag="IWD16_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9089179.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            3544..4050
                     /locus_tag="IWD16_002181"
     CDS             3544..4050
                     /locus_tag="IWD16_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9089180.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4218..6131
                     /locus_tag="IWD16_002182"
     CDS             4218..6131
                     /locus_tag="IWD16_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9089181.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            6136..7137
                     /locus_tag="IWD16_002183"
     CDS             6136..7137
                     /locus_tag="IWD16_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9089182.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(7339..7545)
                     /locus_tag="IWD16_002184"
     CDS             complement(7339..7545)
                     /locus_tag="IWD16_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9089183.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2679 a         1231 c         1561 g         2213 t
ORIGIN      
        1 attcaacaag cagaatcgtt ttgcgaaagg tatccagaat ataaattatc ttttctttca
       61 gaaaacaaga tgataaaagt cgagttttct tctcagtttt tactgagtaa agtttattct
      121 atacttttag agggaacgat tggttatatt ttactggatt cactttacaa aggtacttat
      181 caatccttag aaaatcttag tcaaaaaatt atataagttt gcgtacgatt caaagaaaat
      241 taagagaact taatactatt ttgaaaaact atcatttatc ttgcagcttg aaaaaaggta
      301 atccgataga aggagaagaa taccgtatcc gatacttttt tcacttgatg tattggcagt
      361 cattcgacga aaatcattct gatatattat caagtataaa aaataaacgt caactattag
      421 aaaaatttgc gaaacatgcg ccatatacgc gtagtataga tagaaagaaa tggatcagcc
      481 tactaagaat ttcgttgcaa cgaatgaaac aaggccactc gattacgtat gtcccagaag
      541 agatgaacaa atttgtgcat ccattgataa caaaagaaga gtttacgcat tacatactag
      601 agcctttttt tgaagcaaac ttctggtcga tgaatcacat cactccaaca gagcaagctt
      661 acctttattt tatgtttggt gtaatgaaca cctatttgga agaagatttg ttggatactc
      721 cagataaagt acaagaaagt tggggctgga ttgaatcgtc tattgtagaa aattttgaaa
      781 aaaacttcaa gatttgtttt acaacttcag aaaaaaatta tctatatgtc aatttggcaa
      841 tgatccattt gtattcaagg gtttttggga caaaaaagaa ggtggatgct tttggaaaat
      901 cagcagaaga cagagatttt cagcatatat ttccagtgat gtatccaatc atgcaacagt
      961 tttttagtgg ggtagctcgc agaattccag agcttaatac ttattatgaa aagaacagac
     1021 ggttgatttt tcaatactgt atgttagtca gggatatttt tataaaacac gaacaacctg
     1081 tcatattcta tattcaatca aagtatggta aaacacagga attatgggca aagaaaagga
     1141 tattatctat gactgaacgc ccaataaaat atagtgcatc cgctgatcag ttacctgata
     1201 ttgtcattag cgattatcct atcgacaaaa aggcatatga tcaaacgaat acacagatat
     1261 tttactggaa tggacaacct acaaaaaatg attgggcgcg tctgaaagct gaaatcataa
     1321 aaatcaggaa tgagaaaaat agatccatat tggaaaaatt ggatttcgtt aattaagaaa
     1381 agcggctctt cgtcaatgag tgttggtgaa gaaattcaat agaataatcg agatactcac
     1441 acggctagac agctatcttg tctagccgtg ttgttgttct ggctttagtc agttgggtag
     1501 tgttcttagt tttcttgaaa tattttcctc gatataaaaa gattcgttct cggtagtttt
     1561 gaaagtttct aaagccatag gcaattcgtt ttagtacttt gatatggttg ttcaaacatt
     1621 ctatagggcc gttggaatag ggaactcgca acgctcgttt gatttctgtt cgatatttct
     1681 tgaaggtcgt gaacactggt tgatagtgct ctggcaactc cttgtagtct tcttttagga
     1741 gcgcgacgaa ttcaggaaca tcttgtcttc tgatagcgga aagaaaaacc tggtagactt
     1801 cgtagcctcg ctttaaggag tcatcataat ataatagccg atcgactatc tccgcttctg
     1861 tcagatgatc tcgaaatgac ggacgccagt agcgatgctg accattcaat tcccacgcat
     1921 tcttttgaag tagtctccag tatttcttta agtgcttcga ccgacgatcc ccttttttaa
     1981 aggtcttcat tacttgaatt cggtggttgg taaaggcccg accgatgtgt tggctaatat
     2041 gaaagcgatc ggcgacgatt cgggcattcg gaaaacactc tttcaccaat gtgcgatagg
     2101 gtgtatagaa atctgaaacc actagacgta cttgttcacg gacttttcta gggtagcgca
     2161 agaaatagtc gcgtaaagga ttgagttgcc gattttccac aatatcaatc agttgatggg
     2221 tcttgccgtc catcatgaca aagctcatgg ctcccgagac gtttttcacc gatttgaatt
     2281 catcgaaaca caccacttct ggtaacggct ggttcaaaga aacggtttta ggccgaaggc
     2341 tgcgaagtat tcgaatgaca gtggtcggag aaatgtgctt catacgtgca atcaaagaca
     2401 tagaaatagg ctcacttaat agctcaagaa tggcttgttt gactctgcgg gcaatggagc
     2461 agcgacgatc gctaactgaa tcttctgcta aaaaagtctt tccacagtgt ttacatttga
     2521 agcgctgttt gttaatccgc acaatggtct tgtagttact gacatcattc aataagatgg
     2581 atactttttt ccagccccat ttaatgatcg tttgcttatt ttgatagtgg caatggaagc
     2641 attcctctgg ttgataggtc aatgtgccat caaaaacaaa acatgtttcg tcattaatgc
     2701 gctctttgcg aaaggcatct tcatgaaagg taagatttag gtctagaata tctagtaact
     2761 ctttggtaaa ctctgaatag gacatcttga aacactcctt tgaatggttt tcgtcgactt
     2821 tattctaaag gatttcaagg tgtctgtttg cgtttattag aaaaaagaaa aagtattggc
     2881 aacagaaggc gtcaccaaca ctagatatta tagatccaga aaagcagatt gtttatgcca
     2941 aaattaagac attgcaaaaa gattattttc tttaaaatcg acaagatgac cagaaagcaa
     3001 gagttcgttg agctcttgct ttcttttagt tttagaaagg aacatcctga ttttatcttc
     3061 gctaaatcca gtcaaaagtt ttgcagattg gtcgtagata atcggttttt tcaataaaga
     3121 aggggatgta cgaatctcaa tatatagttc actcaaactg agttcatcta ttctgtgaac
     3181 aagattttta taactaatag aatgagtaga gagcatttcg gtcacgccgt tgtttgtcaa
     3241 ttttagtaag tggataaatt catttttggt tagtggttga cgaattaagt tctgttcgat
     3301 aaacggtata tcatggacga taagccaacg ttttgcgcgt cttgaagacg agcagccaga
     3361 acctgtatac aatttaatca tcatacattc ctccaatagc taagtattta tgaaataata
     3421 acatgaagaa atagtcgaaa agtgccattt agaggtgtaa aataagaaaa tgttgtcacg
     3481 tataatggaa ctagctagat aagaaatact attaaatcaa gaaaaaagaa agaaggcaca
     3541 agtatgtcaa acacagccga cgcaatagta gtgatcgatt tacaaaaagg ggtatgtttt
     3601 gattcttcgc cgatacacga attagaaaca gtgacagcat tagtgaataa aagaatcaaa
     3661 acatacgaaa cgattgggaa accagttatt ttcgttcaac acgaagatgc tcaattagtg
     3721 ccacaaacaa aaccatggga gctattgtcg gaattatcta ttcctgagca cagttatttt
     3781 gtgagaaaaa cccatgcgaa ttcattttat aagactaatt tgagtgtttt attaactgaa
     3841 ttggactgtc attcgatcga attatgtggg gcacaagcag agtattgtat agatacgact
     3901 gtgaagtttg ctcatggatt aggataccag cttatgatgt gtagaggagc aacgacaact
     3961 tatgataatc cttttatgaa agcaaaggaa acaatcgctt tttacgagaa aatttgggat
     4021 aaacgatttc tgacattttt ggagcaataa gttgtttctt cgtttaaaaa aagcagaatg
     4081 gatactggtt tatccattct gctttattac gtaaaatgag aaaataatcc tgttctattt
     4141 ttatgggata aaccatttat tttttgtata aaaaatgttt gcgtttacat ataattctcc
     4201 tagaaacata aaaaggagtg aatagcttga gaacaaaaca agtattaaag ggagcgttgg
     4261 tcatcttatt tgctgtaagt acaagcgtcc cgcaatggac agtatatgga gagagcattt
     4321 cttccgaatc ggaatctcgg gaggtagaaa ccacagaaac tactgatgct ggaaatgaaa
     4381 cgaaatcgag tacttcacaa acagaaacaa atactgaaac aacggttgag acatctgtta
     4441 ctagcacaga tcaagaaacg accgctacca cagataacca acaaacagaa gaagcaacag
     4501 ctacaaaaat gtcaaacgac gaatcctctt ctgctaaagt aagtgacaaa gagaatggga
     4561 cagaaaatga tgatatcggt aaaggtgaaa atttcaatgg taaagaatat ttgaattact
     4621 tcggtaaaca gatgacagaa atacttaatg gaccaacaga agacatcaag tcaaaattaa
     4681 tagagttatt agtcactgag cttgctgtta atgattatgg tgtagataaa gagattatca
     4741 aacatctgat tgctacttat ctattgtacg atatcgcttt tgcaaaaaac agtgtgttga
     4801 tgtcgatgct tggaggtaca gatgatggga caacgcctac agaaaacaca ggcttctatc
     4861 caaatacaaa acaacttcat caaactgtac taaacaaaca agatggcaaa atgatggatg
     4921 tatatgcata ttatgtcgat caaggttcag ataaaacagt catgatccat ggcggatttc
     4981 gagggaattg gaataacggg atcgttacag aagaatacaa tgatttttat aaggccgggt
     5041 ataatttatt atttgttgat tccagagcga ctggtaatag cggaggcgat tatgtgacct
     5101 atggacaata tgagtcagat gacgtccttt actggataaa tcaagaagtt cgagagcggc
     5161 catctcaaaa gattttactt tatggtggtt cgatgggagc agctacgatg atgtctgtat
     5221 tagcgaaaga tattcctgtc aatgtcaaag gaattattga aaattgcgga tttgcatcaa
     5281 ttgatgaaca actacgtttt acgtacagcc agacagtagc ccccgcacta ccggatgcca
     5341 taaaaaatca gttagacatc attggggatc aagagcatga agatttattt atgggccttt
     5401 tgaaacaata ctattttgat caggagatgc atttggatac aaaagctgct ctacctacga
     5461 ttggaatgtc agattcattg cctaaattga ttatccatgg aacagcagat gatgtagtac
     5521 ctgtgtctaa cgcgcaaaaa ttatatgagt tatcaggagg gtataaagat cttttattag
     5581 tagaaggagc agggcatgga aaggcacagg aagttgacca tgctgcctac acgaaacatg
     5641 taacggactt tttgaaagta gtgttccatg atcagataaa cgtaaagtat gtggatgaaa
     5701 acaataaaag tctactcaaa gaccaggacg aaatccagct ttacggagct tatggagaaa
     5761 attatatgac agagcaaaag acattcgaag gatacgaatt agcaaatgtt gaaggaccga
     5821 ctgaagggat ttttaatgag acgataccaa ctattatttt ctattacaaa aaaatcccag
     5881 tagctccacc aaaaaagcaa gatccgacag agaatgcaga caataaggga aagaatgaac
     5941 ctcgagagga tcaatcaagt gatgatacgc aaccgattca gatgacagtg aaagtacaaa
     6001 aaaagacaga tgcaaaaaaa agaatgttgc cgaaaactgg agaaaagaaa cattcgcttc
     6061 tcatttattc aggtggattt ttatctgtta gcacgattat cacatggatt tggcgaagaa
     6121 agagagggta aatgaatgaa atggttcaag cgaatagtga ttgcagtacc agttatcatc
     6181 gtagttattt tagtcactgt atttttgatt aatcaaatca caccaaagcc gattagcttg
     6241 cttgttagaa aacaatttga cacaagtgaa aaagaacaag tatatgcccg accagatgat
     6301 ttccagcaaa aaatcgaaca aatcgatata aaaaaagaaa tatcctatcc ttcaaagtac
     6361 aaaaataata atatggatat ctacacaccg aaagtggaaa agaaaaaatt accgattctt
     6421 ttctggatgc atggaggcgc atatgtagga ggggataaaa atgattgtcg tgattatttg
     6481 gaatatctat gttcagacac gcagcaaatc atcgtaaata tagattatga acgatcaccg
     6541 gaagcaaaac atcctacacc agttatccaa ttgaacgaag ccattcaagc agctaaaaaa
     6601 gagataaaag atcaagcaga ttggtcgaat atttcgattg gtggaaattc ggcaggtgca
     6661 caaattagtg gagaatattt gttagcactt caaaatgaag aaattaaaaa agctgatcat
     6721 cttgatccga ctttggagaa caagcagatt acgaagttta tttctttgtc tggtctgttg
     6781 gatccttctg cacttactca agtaagtgat aaaatctcta gctttcttta tgaaaaatgt
     6841 ggatgggctt actttgataa taaggacttt gaacaatcag agaaggtcaa aagtttagcc
     6901 ttggtcagac atgcggatcg ttggacgcaa aacacattct taacggatgg aaatacaaat
     6961 acgtttacga aacaaatgga agaagtaact tctgtatttg agaaagcggg ggtgaaagtc
     7021 acaaaagtag attatgcgga gaaaaatgct gttttgaacc atgaatatca gtttgatttc
     7081 agcaataaac aagcgcaaga aacatatcaa aagttagtaa tgtttttcaa ggagtaaagt
     7141 cttatgatgc aaaagaggtt gtaatctgtc acttagctcc aagtcacaag gagcaatttc
     7201 aataaacaag cttttcatgg tttatgaata ttgtaacttg tgactgagga gttagagatg
     7261 gcaaggggca ccttttattc gaaatttggg aatatcacac gattattgtc acgatctctt
     7321 ttacgtaaat tttataagtc agatttgttg ttttgatcgt ttaaaaaaag caacaaatcc
     7381 gaagcctgcg gaggcaagaa gggacaaaaa gccgagtggt aagcaaaaga atgctggttc
     7441 cactaatatt ccgttgttat tgatataagg ctgggaacaa agatgaatag caaataaaat
     7501 caatgcacta gcgaaaaaaa gcaaagaaat atggatagct ttcataagca gcacctcctt
     7561 ttttataatt gtatgataaa tagattaaga atctataaat gtgacaagtt tgttaggatg
     7621 tgaataaaat gatcatctag cgaacttgaa acaaagatgg ctaatgataa gcaattaatg
     7681 attc
//
LOCUS       DACDMM010000129         7316 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDMM010000129 DACDMM010000000
VERSION     DACDMM010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.130"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            51..1478
                     /locus_tag="IWD16_002185"
     CDS             51..1478
                     /locus_tag="IWD16_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9089184.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1570..1935)
                     /locus_tag="IWD16_002186"
     CDS             complement(1570..1935)
                     /locus_tag="IWD16_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9089185.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1932..2297)
                     /locus_tag="IWD16_002187"
     CDS             complement(1932..2297)
                     /locus_tag="IWD16_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9089186.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2489..2737)
                     /locus_tag="IWD16_002188"
     CDS             complement(2489..2737)
                     /locus_tag="IWD16_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089187.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2736..2864
                     /locus_tag="IWD16_002189"
     CDS             2736..2864
                     /locus_tag="IWD16_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP9089188.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2921..4615
                     /locus_tag="IWD16_002190"
     CDS             2921..4615
                     /locus_tag="IWD16_002190"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP9089189.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4682..4888
                     /locus_tag="IWD16_002191"
     CDS             4682..4888
                     /locus_tag="IWD16_002191"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089190.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5266..6399
                     /locus_tag="IWD16_002192"
     CDS             5266..6399
                     /locus_tag="IWD16_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP9089191.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6430..6750
                     /locus_tag="IWD16_002193"
     CDS             6430..6750
                     /locus_tag="IWD16_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089192.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6779..7243)
                     /locus_tag="IWD16_002194"
     CDS             complement(6779..7243)
                     /locus_tag="IWD16_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9089193.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
BASE COUNT         2386 a         1408 c         1317 g         2205 t
ORIGIN      
        1 tcataatatt tcccatattg tgcaaaaaaa tacaaggcgg aggctctact atgattgaag
       61 aatatattga gaaaaatatt ttaagacagc tgtttttatg tgggcaattc tatgtaaata
      121 aagaagtcaa tctagaaaag ctctccaatc tcctgcatgt ttgcaaaacc actttactca
      181 atgatatcaa taacataaaa aaagaatttg aagaacagat cgcttataca catcgggaaa
      241 aggaccgtta cactttatat ttttcagaac atatacctcg atgcaagatc atgcagcaat
      301 tgtctcaaaa ttcgttgttt ttaaaaactt gtctgcttta tttggaagaa gatgaacctg
      361 attatcttca attaactgaa tgtgaattca tctctgtcag caaagcttat tcattaaaaa
      421 aacaagtatt agcttacttt aatgactgcg ggatcgaaat cgaccgctac tcccctcgat
      481 tcactgaaat ggaaagacgt ctgctgcttc ttaatgtgtc gtatcgttta ggcggtttta
      541 attcttggga gctacctgaa tcgttttttg aacgtgccga tcgtttcatt gaatccgtaa
      601 cggaaaatag tggcagattt tatgataaag aaaataaaga aatcttatcg attggttttg
      661 ctatcagttt cctacgccag caagtgtgtg ctgtgacaat agattcaaaa tttatcgaag
      721 aaatcaaaaa acgtcctatt tacaattatg tcgaatcagc atgggaaaac actgatttcc
      781 aaacctatta taaaaaagaa gaattcgcct ttattttgac actattcaat ctttgtaatt
      841 atggtttcca ttcttatcag ttgattgcgg aagactttca acagctacac caagtgttta
      901 ttgacaatac tcccgaaata aaagaactag ttgctacctt tgaatctcac ttcaatcaag
      961 aattattcgg aaaccaaccg ttcgaacgtg cgctgattca tctgatgcgt tcggcttggg
     1021 ataactatca attattcatg ccagaaaaat tttatctttt aaatgaggaa cagacaaatt
     1081 tatataaaga agtccagaca attttttcca gctggtccag ccagttacct tatgatctac
     1141 ggttgaatcc taattgcatg cgggcttttg tgatcgaatt gagcggaatt ctccgtctga
     1201 caaaagaaca tctgactatc tatatcgtca caaattcgga tgttcattat ctgatttatc
     1261 gggaagcctt ggaagctgta actactttcg attttcaagt tgcaccaaca atttattcaa
     1321 gtatatctga tataaagaag tatgcgcagc agtcctcaaa ccgggtatta tgtgaacgta
     1381 cattatatac accagatgcg gtccaatatg aaaatattat tcctatttcg atcaatacga
     1441 tcgatcgagc tatcatttca gctgtgcaaa acaaataagg ggctgtgaca aaaatcatgt
     1501 cacagcctct tatttgttga agatgcccat ttctgtaaca atctcttgat acacgtcagg
     1561 ctcacaacct cacttatttt ggacatctag gggtccgatc tctttattta aaaaataaga
     1621 aatgatggtt cggataacta ccaacgccgc tagttggaaa atatcctgca aggtaggttt
     1681 gataatcgat tcgatgatcc cagctgaaac aagaatttcc aaacttagaa gtatatagcc
     1741 tcccaacatc tttttaatag cattgttctc gcttactcgg tcgctttgca cttttgtatg
     1801 gacaaataaa tatttcaaaa aatccttgat tgatagacaa acaccccaaa ccaataaaca
     1861 gatagcaaag atatcaagtc ccagtacaag tttttcgaaa tatggaagaa gaaactgcat
     1921 caactctgtc gtcatcctac ctctcctctt ctttgttttt ctttgtcttc tatttcctga
     1981 ttcaagaaat aagaaatgac tgttcgaatg ataataataa gtgcaagctt caaaatatct
     2041 gtccaagtcg gcttgataac tgactcgatg atatcagcaa cgactagtac ttccaagctt
     2101 aacaaaatat aacttgctag cagttttctt gtatctgcat tacgagtatt acgttctgca
     2161 tagtttttct tgacatgaga ccaatcaaaa agtttgaata gaacaagaac gatcccgaca
     2221 attaagatac cgatagaaca aatatttaaa atagcaatga tccattctag aaattcaatt
     2281 aaatttgagc gaaacatata cgctacctac tttcttttaa tttgttccta ttcttttatt
     2341 gtaaaagaaa gttaccttgt tcgtctattt aaaagttgtt ctaatgataa cggttcgctt
     2401 aaatttttct ttttcactct cccatttttc tccctccatc cataaatcgt ggaagtatcc
     2461 aaaacaacgt agcaagttaa aataaggctt agtccgtcat cagcttttca aagaagcact
     2521 gtctcggtgc tttttttgta cccaaatttc taaaaataga accatacacc tcctcaaatc
     2581 ttttcttctt acctacccta tttttattta caggaaaatc agacacttct tcttttttgt
     2641 caaaatttcc tttttcttct cgtcctatca tttggcattc taataaaaac gaactatcct
     2701 tggatataga aagagaaaac aaggaggcgt ttttcatgag taaaaaatta atcaaagtcg
     2761 gcatcggttt aggtttgctt gcccttggcg ctgcatatct aggaaaaaag acaggactat
     2821 tcgaagatga cagtcattta tatgatgaat tcgaatcaat ctagcatcct atttagttga
     2881 atcactacgg acatacttgg aagacattgg aggaatcaca atgagaaaaa gtaaagcaat
     2941 aatgattggt gccggtattg ccaatatggc agcggctgtt tatttgattc aagaaggcaa
     3001 atggaaaggg gatcagatca ccttttattc attggatgat cacggttcaa atgatggtgc
     3061 accgaccgac actgtagtgg atgaatattg gaataaaaac catccattag aaaaccaaaa
     3121 aggctatatt gctcgaggcg gtcgcatgct taattaccgt acctatgtag atttgatgga
     3181 tctgttaagt cgaatacctt ctgttactga gccagggatg actgctgaag aagatacgag
     3241 agattttgat gcaaaacacc gaacctttga caaagcacgt ttgatggaag gtggcaaagg
     3301 aatcattgat ggcggaaaac tgggattcaa taataaagac cgtattcttc tgactaaact
     3361 gattgctatg cctgattcag aagaagaaaa actagacaat atcactatcg aagaatattt
     3421 caaagatgat ccacactttt ttgaaacaaa cttctggttt atgtgggaaa caacatttgc
     3481 tttcagaaca cgtagttctg ctcaagaatt gcgtcgctat atgcatcaaa tgatttatga
     3541 attcactcaa atcgaacatc tagtgggcgt caatcgaaca cgttacaatc aatacgaaag
     3601 tattatgctt cctttgatcg agtacttgaa agaacaagga tgcaaaatca ttttgaaccg
     3661 ccgtgtcact gactgggaat tcaaagaaac accgatgcaa gatgaaatca ctgtgactgg
     3721 tttgaaaatt accaatacac tgactcaaga agaagaatac gttgtggtcg atgaagatac
     3781 agcagttatt ttcactaatg ggtccattac agattcagct actttaggag attacgaaac
     3841 accagctgta gaaaatatgg attatggtgc agcttctagt ctctggaaaa aagccagtga
     3901 acgtttctac aacttgggaa atcctgacaa attctttgct gatcgagatg caagcgaatg
     3961 ggtcagcttt accctgacga cgaaaaacca tttgttgttg aatgaaatca cccgtatcac
     4021 gactcaagaa ccaggaaatg ccttgaattc atttatttca accacaccga tcacgccgtt
     4081 aggccaaaaa gatgtcaata tgtctattgt cgtgcatcat caaccgcatt ttacgtctca
     4141 gaaaccaaat gaaacagtta tttggggtta tttcctttat ccaagacgac gtggcgaatt
     4201 tgtggataaa cagtatatta aaatgaatgg aaaagaaatg ctagaagaac tgattggaca
     4261 actttcaaaa gtagatcctg gaccagtgaa tattcgtgaa aaagaaacag aaattttcga
     4321 cagcgtaata aataatatcc ctgtctatat gccttatgct tccgcgttgt tcaataatcg
     4381 tgccaaaagc gatcgaccaa aagtcattcc taaacattcg accaatctgg cctttacagg
     4441 agaatttgtc gagcagcctt atcagatgat ctttactgaa caaagtgctg tacgttctgg
     4501 agaaatcgct gctttccatt ttgcgggtat tcctatgtcc cgtcttgtca aaacaccgcg
     4561 ttacgacaaa gatattccta ctctagctcg tgccgcaaaa aaaatgtttg aataatcaag
     4621 aggttgtgaa aaagctgccc tgcatcaagt aataagaaca accaggctaa aacagcttct
     4681 tatgtttttg atttggttgg acttattacc gcagatgcaa gcttttgaac accgtttacc
     4741 aagaggttat gaaaaagctg ccctgcatca agtaataaga gcagccaagc taaaacagct
     4801 tcttatgttt ttgacttggt tagacttatt accgcagatg caagcttttg aacaccgttt
     4861 accaagaggt tatgaaaaag gcgtttagtt tcgagtatga agaaaaattt tgtgaaaatg
     4921 gttcctcacg tttttgcata aatttgactt aataccgaga aactgatccc ttctgtcata
     4981 acctcttttt atttatgtct gtttcttttt gaaaacaact attttttcaa cgtttactct
     5041 cttgccaatc caacatcatc taacggatac aactatttca atgattacca ttacactaaa
     5101 aaatcggaaa cacacctctc cgaacacaaa tacataagac tatagaatgt ttcatttaag
     5161 aaaaaaagcg tttcattatc aaaaaggtaa gatctccttt aaaatgaaat tatctgtagt
     5221 actaataatg cagacaattt tatgaatctg aaaggagcat ctccaatgaa agcagtcact
     5281 tggcaaggaa aacagaaaat ggaaatcaga aacgtagatg atccaaaaat attagaacca
     5341 acagatgcaa tcatccgtat cacagcaaca gccatttgcg gctccgatct gcatctttac
     5401 catcatggtg aagcagtttt ggaaaaaggg tatgttgtag gacacgaacc aatgggaatc
     5461 gttgaagaag tgggatctca agttcaaaca ttgaaaaaag ggcaacgtgt ggttatccct
     5521 tttaatatta gttgtggtca ttgtcatttc tgcctgaatc acatggaaag ccaatgtgat
     5581 gaatcaaatc cagatcagtt atatggcggg cttttcggat ttggcaagtt gaacggtaat
     5641 cattggggtg gtcaagcgga atatctaaga gttccttatg cagactctac ttcatttgtt
     5701 gtaccagata atgatcttcc tgatgaaaaa gttttatttc tatcagatat tttgcccaca
     5761 gcttattgga gcgttgaaaa cgcaggagta aaaaaaggcg atacagttgt gattctaggt
     5821 tctggtccag tcggtctatt cgctcaaaaa ttcgctgtta tggctggagc agagcgcgta
     5881 atcgcagtag atccagtaca gcaccgtcta gataaagcgg ctaattacaa tggggttgaa
     5941 acttatttct tagaagacac ggcacaagct ggaacagaat tgtatgaact ggctaaaggt
     6001 ggcgcagatg tcattatcga ttgtgtagga atggacggat tagagcctgt caaagaaaaa
     6061 gcgaaaaatt tagtaagctt acaaagcggc acaatctctc cattacagat ggcatcgcaa
     6121 gctgtcaaaa aatttggaac agtccaagta actggtgttt atatgactcc agcttcttct
     6181 tacccgctgc aagaattttt tatgcgggat atcgctgtca agcatggaca ggcaccaatt
     6241 atccatctaa tgccaaaaat ttacgcaatg attgcagaag gactattcga tcctacacaa
     6301 atcattactc attcgctgcc actatcagaa gcagcacaag catatgagat ctttgacaaa
     6361 aaagaagata aaaatataaa agttgttttg aaaccttgaa ttcagtaaaa aatacaggtg
     6421 attttcttta tgcccaaaat caaacgaaat acccatgaga tcgaaatagg aaaagaagaa
     6481 gatatcgaaa tacgaggacg tcgcttccgc ttctatttcc aaaatcgtta cacattgatc
     6541 tcactggctg tcgatttatt aaccgggatt ttctatattt ttggaagcct tgcttcctta
     6601 actaaaatcc ccgatcgata tggcacctat ttttatttgg ctggtgctat tttcttgacg
     6661 attcgtccta ttctcagaat cgtaaggaac atttttattt acgacgaaaa aaaagcaatg
     6721 aagagaacaa gcaaaaaaaa agaggcatga gaatttttca aatctcatgc ctcttttttc
     6781 accctatcgt atcactttga tacagacgag cggtcgtccc actttcttcc ttgaccattg
     6841 tcaaaaatgt ccactcgtat cgttcataaa acgtggtatg atctgtaatt aagtaaagtt
     6901 tttcgtaccc aagttgatgc atttcttttc tgatttccgc taatatccgc ttggctatcc
     6961 cctgcttacg cttttccggt tcaacaaaca gggcacaaag atttggtgtc agatcagggc
     7021 ggttatggaa gtcgttttca atgacgcccg ctcctccgac gatggtttct ttttcatcta
     7081 agaccacata ccattgagga atactttcct tattagcgat tccagtacga atactttcct
     7141 catacacaac agcaggaact ccccattttg atgaaaacca attcgcgctt tgttttacta
     7201 attctgggtg ttctcttaat ttgatccagt tatatgtaac caatgtttaa acctccgact
     7261 aaaataaaac gtttacaaaa attatacaag tctttatgaa aattagcaat gacaac
//
LOCUS       DACDMM010000130         7310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDMM010000130 DACDMM010000000
VERSION     DACDMM010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.131"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1389
                     /locus_tag="IWD16_002195"
     CDS             <1..1389
                     /locus_tag="IWD16_002195"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9089194.1"
                     /translation="LLSDEESKEDLIATIVDILGYAIAPEYLFNVLADQAKQATFQLN
                     DLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDG
                     DVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGS
                     GSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDW
                     PTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLK
                     ETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKK
                     NRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKEN
                     DYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAW
                     LQGALEVFKHEQ"
     gene            1379..2596
                     /locus_tag="IWD16_002196"
     CDS             1379..2596
                     /locus_tag="IWD16_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9089195.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2609..5758
                     /locus_tag="IWD16_002197"
     CDS             2609..5758
                     /locus_tag="IWD16_002197"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP9089196.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         2551 a         1192 c         1469 g         2098 t
ORIGIN      
        1 ttactttcag atgaagaatc aaaagaagat ctgattgcca caatcgttga tattttggga
       61 tatgcaattg caccagagta tcttttcaac gtcttagcgg atcaagcaaa acaagcaacg
      121 ttccaattga acgatctaaa caaagccttt gtgcaattgg catctaccta caatcaattt
      181 aatgggttat ttgatgatgt ggatctccaa tcaaaaaaac taggaacaga tgagcaacaa
      241 cgaaatgtga cgattaccga agtcatcaag aaattgaatg atgtagatgt tttagaacac
      301 gatggggacg tgatcgggga tgcgtatgag tttctgatca gtcaatttgc ttcagaagca
      361 gggaagaaag ccggagaatt ttacacacct catatggttt ctgatatgat ggcgcaaatc
      421 gtaacgctcg atcaaaagga acgacgattc tttagtgttt ttgatccaac gatgggttca
      481 ggttcattga tgttaaacgt acgtaactac ttaacacacc cagataacgt taaatatcat
      541 gggcaagaac taaatacaac gacatacaat ctagcaaaaa tgaacttgat tttacatggt
      601 gtcgacgccg aagaaatgaa tctccgcaat ggggatacgt tgaataagga ctggccaaca
      661 gatgaacctt acacatttga tgcagtagta atgaacccac cttattcggc taattggtca
      721 gcagatacga ctttcttaga tgattctcgg tttaatcgtt atgggaaatt agcgccaaaa
      781 tccaaagcag attttgcgtt tcttcttcat gggttctatc atttgaagga aacaggtacc
      841 atggccatcg tcttaccaca cggagtttta ttccgtggcg ctgcagaagg ggtcatccgt
      901 caaaaattac tagaagatgg cagtatttat gctgtgatcg gtatgcctgc gaacttgttc
      961 tttgggacat cgattccaac gacggtgatt gtgttgaaga agaatcgtca aactcgagat
     1021 gtattattca ttgatgctag tcgtgaattt gttaaaggga aaaatcaaaa taaactatcg
     1081 gaagaaaaca ttcaaaaaat cctcgaaacc tacgctgaaa gaaaagatgt cgagaaatac
     1141 gctcatttgg caacctttga tgaaatcaaa gaaaatgact ataacttaaa tatcccacgc
     1201 tatgtagata ctttcgaaga agaagaacca attgatatgg ttcatgtcgg aaatgacatc
     1261 aagaagatac gacaagaaca gcaagtgcta gaaaaaaaac ttttagaagc tatctcttcc
     1321 ctgcaaacaa cacctgaaaa tgaagcgtgg ttacaaggtg cgctggaggt gttcaaacat
     1381 gagcaatgat acacaaccag aaatccgttt cccaggattt acagatgatt gggaacagag
     1441 gaagttgggg gacttcgttg tagattatgt tgaaaagaca agtgtacaaa atcaatttcc
     1501 tatgctaact tcatctcaac aaaaagggat agttttgcaa gaagactatt ttgcaaatcg
     1561 gcaagtaact acagaaaata atattggtta ctttgtacta cctagaggat atttcacgtt
     1621 ccgtagtcga agtgataatg atgtgtttgt gtttaaccgt aatgacatta ttgatagagg
     1681 gattataagc tatttttatc ctgtatttac tttaaaatct gctgattccg acttcttctt
     1741 aagacgtatt aacaatggaa tccaacgaca attatctatt caagcagagg gaacaggtca
     1801 acatgttctg tctctaaaaa agtttaaaaa tatagtagca atgtttccta gtgaggaaga
     1861 acaacaaaaa atcggcacct tcttcaaaca acttgacgac acgatcgctc ttcatcagcg
     1921 taagttagat ttgctgaagg aaacgaaaaa aggattctta caaaaaatgt tccctgaaaa
     1981 cggagcaaaa gtgccagaaa tccggtttcc tggttttacg gaagattggg aagagcgtaa
     2041 gttgggagaa ttaggtaaaa ctcaatcggg aattggattt cctgatgccg agcaaggtgg
     2101 ttctgaaggt actccatttt ttaaagtttc cgacatgaat aacattggta atgagtatga
     2161 aatgagaaat gcaaatcatt atgtatctaa tgagcaaatt gagcgtaaaa aatggaagcc
     2221 aataaaagat gtgccagcga ttatatttgc taaagttggc gcagcaataa tgcttaatag
     2281 aaaacgtttg gtcacaagcc ctttcttaat agataacaat accatggctt atttatttaa
     2341 taatacttgg gatatctatt ttggtaaaat attgtttgaa actataaatt tgccaagata
     2401 ctcccaagta ggggcattgc cttcttataa cagttctgat attgagaata tcagtgttaa
     2461 ggttcctgta aaggatgaac aacaaaaaat cggtactttc ttcaaacagc tagatgatac
     2521 gatcgctctt catcaacgta agttagattt actaaaagaa acgaaaaaaa gcttcttaca
     2581 aaagatgttt gtctaggagg gaagaagaat gaaaattagt caccgtgagg aagcagaagt
     2641 cgaagaacag ttgattcgtg tacttggaga agggcataat cagtggacgt atcgtccgga
     2701 tctaaaatca gaagaagacc tttgggtcaa tttacgccaa aaaattatct caaataatca
     2761 agcagaactg aatgattttc ctttgaccga taaagaattt gaaacgatca aaacggaatt
     2821 actgctgcga acgaaaaccc catttgatgc tgcgaaatgg ttaaaaggtg aaaatgggat
     2881 ggctcggatc acgattgaac gggaagattc gcaattgggt tctgtctcac ttatccttta
     2941 ttccaatcaa gacattggtg gaggaatctc tacttatgaa gtggtccatc aaattgcaaa
     3001 acgtggaagt aacatagaag cacgggatcg acgttttgac gtcaccttat taatcaatgg
     3061 attaccgatt gtccaaatcg agctgaaaca agtaactgca aaagatggtg tttatcaggc
     3121 atttaaccaa atcaaaaaat atgcagaaga aggtatgttt cggaacaata tcttttcgac
     3181 cttacagttg tttgtggttt ccaatgaaca aaccactcgg tattttgcaa atgccttacc
     3241 gaaagacttg caccctaagt ttttatttag ttggcgaacg aaagacaatg aaaaagtaga
     3301 aaacttatat gagttttgta aacaagtgtt aaatatcccg gatgctcatc gtttgattgc
     3361 ggattataca atcgtcagtg aagatcaaga caataaaacc ctcatggtgt tacatcctta
     3421 tcaagtacat gcgattcaag ccttgtttat tgccgcaaat aaacatcaat cagggtatgt
     3481 gtggcatgcg actggttcag gaaagacgtt gactagtttt gtatctacta agcttttagc
     3541 acgaaaatcg gggatcgatc gaacgatcat gttagtggac cgcaaagatt tagataatca
     3601 aaccaccaca gagtttacaa aatttgcttc ggaatttaat actgggattt cttccgggaa
     3661 cgccaaagcg aatagcttga tcgttggaac gggaagtgcc aaagaattaa gtgaaaccct
     3721 gttggcagat gccaatgcaa atgttgtaat cattacaacc cgtcaaaaac tagatgcggc
     3781 gttgaaatat gcaaagaaac aagaagagaa aaaaggaacc aatcgcttcc aaaaattaat
     3841 gggccaacat atcgtgtttg tagtagatga gtgtcatcga gcgttaagtg ctgaaaatat
     3901 ggaagaaatc aaaaaaatgt ttccgaaatc cacttggttt ggctttacag gtacaccgat
     3961 ttttcctgag aatcggaaac aagccaaagg gcaattggca cgtacaaccc atgatcagta
     4021 tggagaggtc ttgcatacct ataccattaa gaatgcgcta gaagatggct ctgtcttagg
     4081 atttcaagta gagcatgaaa acaccgtgga gccgacttca ctagagaata aaatctatcg
     4141 gaaattaaaa gaagtagaaa catatgcaga gtattcatca gaacagatca atcgtatgat
     4201 tgatcaaatg gaacctgtca aaaaagaaag ttatttggac ccagctgttt ttgaagcaga
     4261 tgaacatatc caaaaagtca ttcataaaat gttccgtcca gataatgcct atacaaaatt
     4321 tgattttcga aatggcaggc caacgaaatc tgcaatctta accacgagtt ccattgacat
     4381 ggccaaaaag tattatcggg ccatcaaaga gatgacgaaa gagcctgatt ggttaacaaa
     4441 agagtttccc aatcagctca ttcgtgaagg acgaacgatg gaggatcctg attttccacg
     4501 aatcgctatt acctattcat tagaagaaaa tagccgagat tcaagtgccc aacaagaaga
     4561 aatgcaaaaa attattgagg aatataatca gtattatgat acttcctggt cattagcaga
     4621 cattgagcgt tataacggag acatcaataa tcgtttggca cgaaaaagag cggaattcaa
     4681 acaatttggg aaacaaatcg atttagcgat tgtagtggat cgtctattaa caggttttga
     4741 cgcccctacg attcaaaccc tatttgtgga tcgtaatcta gagtatgcag gattgatcca
     4801 agctttttca cgtacgaatc gtacgtatcc tgataagaca aaaggattga ttgtgacgtt
     4861 tcgtaagcca gcgacaatgg agaagaatgt cgaggatgcg acaaaactgt attcaaaagc
     4921 aaaagaagaa tcaggtttga tttatccaag ttatcaggaa tccaaaaaaa gattcaaaca
     4981 agcatatggt aagttaaaag aggtttcatc catggaatcc attgatgaac atacgccact
     5041 tgaaatccgt atcgagtacg tcaaagcatt tcaagaattg aatcgtgctt atgaatcgtt
     5101 agtgacttat gacgattaca atgatgacat ggtgaaatct ccaactttga acaatcaaat
     5161 tcaacttttg gaagaacaaa ttggcgtgta cgagaccgtg aagggttcat taatagagga
     5221 agaaccagaa aaagaaggag aagatttttc aacgatcgaa ttttatagtg aaaataccac
     5281 taagttgtat gatattgatt caacttatat tgatcaatta ctaggaactt atgctgcgaa
     5341 tagttcagat gcacgagaag aaattgaaaa agctttggct aaattgaata aaacagaagg
     5401 agtcaaagaa gtttaccgac aaattttaaa tgcatttgat gtggggactt tagatagtaa
     5461 tgaagatatc tttgtcatta aacgtcgcta ttttacacaa gcgagtgatg atttgattca
     5521 tgtcttttcc aatgagtggt ttgtttctga gaaagaatta catgcttcta atctgcaata
     5581 tctaccaggt gaagatccaa ttcctaatat gaaagcaatt atcaacagta aggattacga
     5641 aggatataaa gccaaacatc cagaagcgaa gccatttaaa tacccacaag aaatgaagcg
     5701 agcatggcgt aaaatgttag atgatgagtt gattccactt gagaatgaat tgaggtaaac
     5761 gagaagagtt aagaaaattg tacgattcaa agatggtagt caagtggaag tgtagaaaat
     5821 agggatagat gtcttttatt tgttcacatt ttataacgaa gtatttaaga cagctattga
     5881 ataatgtttc ctccaccttt ttcattcatt taccccaccc cgttgttata tgaccgtgtt
     5941 ttgagaggaa ccaataaaaa gcataggcta actaggggtt cctttttctt ttgttttttt
     6001 ggaatccgct taagtgaaat gtatctgaaa tgaaacggag ataaagtgat tttttgctat
     6061 aatgacaagg ttttagctgt ttcagatacc atgtaaccca aaatagttta ttgagaaaat
     6121 aggagagtat ttgatactaa ctagtaggaa aaaatgagat aaaaattaaa tggaatcatt
     6181 tcaggtaatc tcaaataaaa aaatatacag tcctgtggca ctttacctat gggactgtat
     6241 attttttact ctcagcgggc ttgttttgac tccgtgtctg actccgtcgt attgatttta
     6301 aatgatattc gatgaccatt taacaggtca tttttgaaat ttttgtctct atatgactca
     6361 gtttgataat tattgataga tcaatttggg tctatatcga acaagggaat aatccaataa
     6421 aaaatgattt agaatatcaa aataacaaaa aagaggacta cctgtattcg tcctcttttt
     6481 tgaccagatt ttgaccagtc taatttacat tctaatatat tcatatgagc taattttttg
     6541 attaaatggt aagtttttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
     6601 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
     6661 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
     6721 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
     6781 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
     6841 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
     6901 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
     6961 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
     7021 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
     7081 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
     7141 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
     7201 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
     7261 caatccattt tgtattttat tgagtactct tggataaact accagcgatt
//
LOCUS       DACDMM010000131         7289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDMM010000131 DACDMM010000000
VERSION     DACDMM010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.132"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            48..1040
                     /locus_tag="IWD16_002198"
     CDS             48..1040
                     /locus_tag="IWD16_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9089197.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            1056..1940
                     /locus_tag="IWD16_002199"
     CDS             1056..1940
                     /locus_tag="IWD16_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9089198.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            1989..3596
                     /locus_tag="IWD16_002200"
     CDS             1989..3596
                     /locus_tag="IWD16_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9089199.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            3597..5081
                     /locus_tag="IWD16_002201"
     CDS             3597..5081
                     /locus_tag="IWD16_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP9089200.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            5258..6250
                     /locus_tag="IWD16_002202"
     CDS             5258..6250
                     /locus_tag="IWD16_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9089201.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            6302..7171
                     /locus_tag="IWD16_002203"
     CDS             6302..7171
                     /locus_tag="IWD16_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9089202.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         2311 a         1172 c         1620 g         2186 t
ORIGIN      
        1 ttcgttaaat ttttattttt ttaaacgaaa atggaaacgt ttctaaaatg tattgggagg
       61 agattgaatt ggaaaagaca ttgggaccag gtggtgagat aagggcggta caacgggata
      121 aaaaaaatat ttcgaagtgg gaaaaattaa agaaacagtt gcggcgtgat aagtggatgt
      181 acttgttgct cttaccaggt atcatcatga cgtttatctt taagtatgtg ccaatgtacg
      241 gtgctgtgat tgcatttaaa gactataatc ctatgctagg aattttggaa agtccttggg
      301 taggactaga tcactttagg gaatttatca gttcaccaaa ttttacgctt ttgctgagca
      361 acacattaaa aattagtatt tatggtttat tgttaggttt ttttccacca attattttag
      421 cactgagctt gaatcaaatt atcaataaaa aaataaaaca gaaaatccag ttgattctat
      481 atgcgccgaa ttttatctca gtagttatta ttgtggggat gctgtttttg ttcctatccg
      541 caaatggacc tattaatggg ttagtagaaa aggttacggg gcaaccaatt atgttcatgt
      601 ctaatccagc ttatttcaga acgatttata ttttatcggg aatttggcaa ggaatcggct
      661 ggtcatccat tctttacacc gcaacgctgg cgggagtcag taccgattta tatgaggcag
      721 caaatatcga tggggcaaat attttacaaa agatttggca tatcgatttg cctgcattga
      781 ggcctgtgat ggtgatcagc ttcatcttgg ccgccggcgg tattatgaat gtaggatatg
      841 aaaaagccta cttgatgcaa acttcaatga acatccctgc ctcagaaatc atttccactt
      901 atgtatacaa agtcggcttg caatcaggtg attatgccta ctctgctgct gttggtctgt
      961 tcaatatgat tattaatgtc gttttattac tgattgtcaa caatataacc aagcggctaa
     1021 acgatggtca aggcttgtaa tgaaaggaga aaattatgaa tagtgcttta tcaaaaatcg
     1081 accgtcaaat cttatggttg aatcgagtga ttgtaacttt tttagtcgtc atcaccattg
     1141 ttccgctgat ttatgtactg gtagcttctt ttatggaccc agctactttg atcaataaag
     1201 gtataagctt caatccgaaa gattggacaa tacagggata tcaacgggta tttgcagatg
     1261 attccatttt acgcggtttt gtaaattcgt tgttttactc ttttagcttc agcttattga
     1321 ctgtatttat tacgatgatc actgcgtatc ctttgtctcg acctaacttg gcggggaaaa
     1381 agattattat gacttttttt gtcattacaa tgttcgtggg tggtggcttg atacctacct
     1441 atttattggt aaagaacttg ggcatgttag atagtgtatg ggcgattatc gtaccaggtg
     1501 ctataaatgt gtggaatatt atcttagccc gtacctattt tagtgctttg ccaaaagaac
     1561 tttctgaagc tgctactatc gatggcgcca atgacttgca aatactgttc aaagtcatgc
     1621 ttccattagc gaaaccaatt atgttcgtat tgtttttata tgcctttgtt gggcaatgga
     1681 actcatactt tgatgccatg atttacatca aagatcccga cttacagccg ttgcaactcg
     1741 tattaagaaa tattttgatc caaaaccaac cttcacaaga catggtggga gccaatactg
     1801 ccatggcaga aatgaaacaa attgctgagt tgatcaaata cgcaacgatc gtcatttcta
     1861 gtctgccatt aatcataatg tatcctttct tccaaaaata ttttgataaa ggtgttttag
     1921 ttggttctgt taaagggtaa gtattgttca ctatttgtat gaataaaaat tataaaggag
     1981 attttaagat gaaagcaaaa tggatgctgc caatcgtttt tacttccgta ctaattttgg
     2041 gggcttgtgg aggctcaaaa gaaaagagta aatcaagccc tgattatgaa ctagaaaatg
     2101 tctcttttcc attgaaagaa aaagtatctt taaaaatgat gtctcaaagt gcccctttgg
     2161 ctcccaatga tcctaatgac aaattgattt tccaacgatt agaagaagag accaatctcc
     2221 atattgattg gacaaactac agtagtgatt ttgccgaaaa aagaaatttg gatatcgcta
     2281 gtggtgattt accagatgcc atcttcaacg ccgctgcagg tgactatgat ttattaaact
     2341 gggcggaaag tggcgttatt gtaccgattg aagatttgat cgaagattat atgccaaatc
     2401 tgaagaaaat ttttgatgaa aaccccgaat accgacaaat gtctactgca cctgatggtc
     2461 acatttactc tatgccttgg attgaagaat taggcgaagg aaaagagtcg attcatacag
     2521 taaatgggat ggcttggatc aacaaggagt ggttggataa tttgggtctt gaaatgccgc
     2581 aaaccactga tcagttgatg actgtgttgg aggctttcaa gacgcaagat ccaaatggta
     2641 atggtgaagc agatgaaatt ccttttagct ttattaacaa tggaggaaac gaagacttaa
     2701 aatttttaat gggggcattc ggtctaggtg ataatgatga ccacttagtc gttgataacg
     2761 atggtcaggt acaatttact gctaacaaag aagggtataa agaagccatc aaatatttca
     2821 atgaaatgaa taataaaggt cttattgata aagaggcttt cgaacaagat tggaatacgt
     2881 atgttgccaa agggaaagaa cataagtatg gcgtgtactt tacttgggat aaagcaaata
     2941 tcacgggtgc agatgatagt tatgacgtct tgccagtctt agcgggacca gatggaacga
     3001 aacatgttac tcgtacgaat ggcatgggtt tctcaagaga tcgtttcgta attactagtg
     3061 cgaataaaaa cttggaactt acagctaggt ggatagataa aatgtatgaa ccgttacagt
     3121 ctgtccaaaa taactgggga acctacggtg atgaagcaca ggcgaatatt tttgaattaa
     3181 aggatggtat gttaaaacat ttgcctttag aggggacagc accgggggaa ctaagacaaa
     3241 aaaccgaagc tggaggacca ttagcagttc ttgattctta ttacggcaca gtcactacta
     3301 tgccagatga tgcgaagtgg cgtttagatt tgatgcatga cacgtatttg ccatatatta
     3361 cagaagacaa tatctatcca cgtgtctttt tagaaaaaga agaaacagat cgattagcaa
     3421 aaatcgaaac ggatatgtgg gattacatct atcgcaaaag agcagagtgg atcaccggcg
     3481 gtaaaatcga tgaagaatgg gaagaatacc ttgctgaatt ggaacgtgta ggtttggaag
     3541 aatggattca aataaaacaa actggctatg ataatttttt gaagggagca caataagtgt
     3601 tagtagaaga aacggcgatt ttggataaag caaatcgatt tattgaaatg atgcgaggaa
     3661 aagttaaccc tcagtatcga caaaactatc atttttccgc acccatcggg tggataaatg
     3721 accccaatgg gtttatatat tacaaagggc attaccatct cttctatcaa tactatcctt
     3781 acgatagtcg atgggggccc atgcattggg ggcatgcgcg cagtagtgat ttggtccatt
     3841 gggaagatct accagtagcg ttagccccag atcaacctta tgacagcggc gggtgtttct
     3901 caggaagtgc aattgaaaga gacggaaaac tatatttatt ttatacaggc tgtcaagaaa
     3961 ttgatggtat tagccatcag catcaatgtt tggcggtctc tgaagatggt attcatttta
     4021 gcaaatacag tggtaatcct gtggtagcag aaaaacagct aggtgaaaat gggctaatac
     4081 aagaatttcg tgatcctaaa gtaattgttc acaacgatgg ttactatatg gtagtagcta
     4141 ctaaatctac acagtctact ggtagaatag tgatgtttca atcgtcagat ttgtatactt
     4201 ggcactttta ctctgtgctg ctagaaggga cttcagagca aggaataatg tgggaatgtc
     4261 ctgatttatt ccatttagac ggacgagatg tcttaatcat gtctccgata caaatcaaga
     4321 agaacggact ggaatatcat aatatcagtt ctaccatggc ttgtattggt gaggtaaact
     4381 gggaaacagg tcagatgata gttgataatt tccatgaaat ggattttgga atggattttt
     4441 atgcaccaca aacaacaaga gatcctaatg gtggacgggt gatgattgct tggatgcaga
     4501 tgtgggatcg tacattgcct acacatgagt taggccatct ttgggcaggc tctatgagtt
     4561 tgccccgaga gttaagagtg aaaaaaaatc gattggtaca aaaacccatc tcaactgtat
     4621 atagtcattt agtttatcaa caaggcagtg aaaatattgc agtgactgac acatcagtcg
     4681 tatttcgaaa cgcgctgtct gattcttcat atttacatct agtcgcagat ttggagaaag
     4741 ctgaagtttt tacaattgaa ttactgcggt ctaagacgga ttgtctcaaa ctagcctatg
     4801 acactgctac acaggttttt gaattggatc gtcagaaact aggaagagag cttacagggg
     4861 cggaagaaga ccctctgaat gtacgaaaag ttgaagtacc tttgattgat aatcagctcg
     4921 ttcttgagat ttttagagat acctcatcaa ttgaagtctt tattaatggg ttagaagtta
     4981 tgacggctac tttctatgag ctggaaaaag ctcgagatat cttgtttcat tcgatcggag
     5041 aaagtcgtat tcaagctttt gaaacagcga gtgtgaaata aatgtttgat tgcttaggcg
     5101 atgaatcaat tatttaatag gttttgaagg ataatgtttt tagaaaagaa cagatagcta
     5161 gatttcccat ggatttgcta ttagtatttt gaaaaaatta gtaatttatg acaaaattca
     5221 ttatactaaa cttgtgcaga aatgtgaggg atcaactatg aaagcaacaa tgaaagatgt
     5281 cgcaaaatta gcaggtgttg gagtaggaac tgtttcccgg gtattgaata acggttctgt
     5341 taaagcttct actagagaaa aagttgaagc agcaatgaca gagttaaact atcaaccaga
     5401 tttttatgcc cgggggttaa agatgaagca tacgaacacg gttgctttga ttctacccac
     5461 ggtatggcat ccattttttg gcgaatttgc ctaccatgta gaaacagcat tagaaagaca
     5521 aaactacaag ctttatttgt gtaactccag tggtaatagt gaaaaagaac aagaatatat
     5581 ccagatggtc agccaaaata aagtagacgg catcattgga attacttatt cagatatcga
     5641 tgaatacatt tcgagtaatc tgccttttgt tagtattgac cgttatttca ctgaagaggt
     5701 gatttatgtg accgctgaca atgcggccgg tggacgtttg gctgcaaatg aactttatcg
     5761 ccgaggatgt cagaatgtag catatatcgg tgggtaccaa gaaacaccga atgaaacgaa
     5821 gagtcgtcga aaatattttg aaatcgagtg ccgtcaactg gggatttcct ataaagcgtt
     5881 agatatgtta gaacctttag accatctctc agaacgtgta acaaattttt tgttagagaa
     5941 tcccgatatc gatgggattt ttacgatcaa cgatccaatg gcattagctg tcattgatat
     6001 gcttgaaaaa atagggaaaa acgtaccaga ggatgtccaa gttatcggat ttgatggtca
     6061 aaagatgaca gaaggagatc cttatctagt ctcaacgatt gcccaaccta caaagcaaat
     6121 ggcggaagtg gcagtggcaa aattaattgc tgtcattaat ggagagacag tagaaaaacg
     6181 taccgtattg ccgattgaat ttggcgaagg tgacacaacg aaacctttag tgagaaacga
     6241 ttttgcttag tgcaaatacg tttctttttt tgaaaatgag tgaatgaaag gtgtagataa
     6301 aatgttatat ggtggaatag aagccggtgg aacaaagttt gtctgtggga ttggtaatga
     6361 acaattacaa gtgatagaac ggaaaagttt tcctaccaca acaccagaag aaacgatgga
     6421 gcaagtgttt gatttctttg ataagtacaa aggaaatatc gcatcaattg gtgtaggagc
     6481 atttgggcct attgatattc aaatcgaatc aagaacttac ggatatatca ccaacacacc
     6541 gaagttagct tggcaaaatt atcattttgt aggagctttg gaaaacgcct tgtcggttcc
     6601 catctattgg actaccgatg taaatgctgc ttgttatgga gaatatatgg cagggtatgg
     6661 aaaaggtaaa aatagtgttc tgtattacac cgttggtaca ggtgttggtg gtggtgctgt
     6721 agtggatggg aaaatagtcg caggttttag tcatcctgaa atgggacata tgctcttaca
     6781 acgtcataat gaagatcaat ttgaaggaat ctgtcctttt catcatgatt gtttggaagg
     6841 gttagcatca ggacctgcga ttgaaaaacg ctttggaaga aaagcccaga ctatctcatt
     6901 tgatcatcct tattggacga ttgaggcaga ttatcttgct cagtgtgtgt ataacacaac
     6961 gttactatat gcaccggaag taataatttt aggcgggggt gtaatgaaac agccgcaact
     7021 actcgagttg attcgccaat cgtttaaaga aaaaatgggt tcctacgtgg atacgcctac
     7081 tctcgaagaa tatctactaa ctccaaggct tgctgacaat gcgggcttga ttggctgcct
     7141 agctttagca ataaagaaac tcaatcatta gaatagtatt tattagaact attcataaca
     7201 taccggaagt ttaaaagggt attctgaaac ttaaatagtc tcaattcatt cttctttctt
     7261 gagaatctga gtagagactt ttattctgt
//
LOCUS       DACDMM010000132         7173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDMM010000132 DACDMM010000000
VERSION     DACDMM010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.133"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            351..824
                     /locus_tag="IWD16_002204"
     CDS             351..824
                     /locus_tag="IWD16_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP9089203.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            821..2953
                     /gene="feoB"
                     /locus_tag="IWD16_002205"
     CDS             821..2953
                     /gene="feoB"
                     /locus_tag="IWD16_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP9089204.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            2955..3089
                     /locus_tag="IWD16_002206"
     CDS             2955..3089
                     /locus_tag="IWD16_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP9089205.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            3183..3827
                     /locus_tag="IWD16_002207"
     CDS             3183..3827
                     /locus_tag="IWD16_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9089206.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            4014..5207
                     /locus_tag="IWD16_002208"
     CDS             4014..5207
                     /locus_tag="IWD16_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9089207.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            5200..6927
                     /locus_tag="IWD16_002209"
     CDS             5200..6927
                     /locus_tag="IWD16_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9089208.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
BASE COUNT         2330 a         1251 c         1592 g         2000 t
ORIGIN      
        1 aactcttagt aaaaaagtca aacaacaaac actacatatt gtgttgtgga aactgtggaa
       61 tactaagttt tgtgtttttg aaaaaagagc tgaaaacaag tatcgtgtgg gaaaatagca
      121 aatttaatag agaaaaaatt gtggaaaact atgaagaaat atttttttct catttttatc
      181 tatcaatact aaagattcgc tcataatatc acaagtataa ttgacattct caattaccga
      241 gagtattcta tgtgagaatg attctcattt atttgcgtaa cacgtaagaa aattagaaca
      301 tataatgaat gattttaata catgatttat ttcacaagag aggaatcaat atgcagacct
      361 tagcgcaggc aaagcagaat ttcgtttatc aagtcgaacg aatcgaaaca gaagaaaaaa
      421 caaaacgcca tttgcaaaat atcgggatcg ttgccggaag taaaatcgtc gtcgtcaatt
      481 tatcaggcaa caatgccatt ttactggtaa aaagtcatcg agtggcagta agtcgggaaa
      541 tactaggtca aatctttgtc aataaaaaga ctgcttcctc tgaaagctgg acttctcttg
      601 atcaattaaa agcaggagag actgggatcg tcgtagcaat ccacggacaa ggcgcagtga
      661 agcgacggtt aatggatatg ggattgacgc gaaatgtaaa aatagttatc cgaaaactag
      721 cccctctggg agatccaatc gaactgacag tgcgagggta tgaactgacg ttacgaaagt
      781 cagaggccga actaatcttg atacaaaggg aggaaaaaga atgaatatcg cgttagtcgg
      841 aaatccaaac agcgggaaaa ctagcttgtt caatctcttg actggttcga atcaatatgt
      901 agggaattgg ccgggcgtaa cagtagaaaa aaaagaagga tcgttgaaaa aaaatccgaa
      961 cattcatatt caggacctac ccggcatcta ttctttgtct ccttatacac cagaagaagt
     1021 gattgcaaga gattatttgg ttcaggaaca gcctgaactg atcatcaaca ttattgatgc
     1081 taccaattta gaacgaaatc tttatttaac tacgcagtta ttggatttag acattcctat
     1141 gatcattggt ctgaatatga tggatgtcat caaaaaagaa ggaaaaaaaa tcaatctaga
     1201 aaagctttcc tatggtcttg gtgtaccagt tttaccaatc agcgtattga aaaagcaagg
     1261 aatcgaaaaa ctaatcgaaa agattcagga gtctgcaaaa aagcggcctt ctattcctgt
     1321 tccctgtaca tatgatccac gtttagaagc agctttacat gaaattgaag atgtactagg
     1381 agaacaaagc ggcaagaaac gctggtatgc aatgaaatat tttgaacgag atcagaaggt
     1441 aagagaggat tacgagatag cagtttctca gcaaaaagag atcgagcaac tgatccagtt
     1501 aacagagaaa ctattggacg acgatagtga gacgattctg gtcaatgaac gttatgaatt
     1561 tattacccaa ctatgtacac tctgtgttgt gtcaaatgat tctttccaat tatcgatgag
     1621 tgacaaaatc gatcagattg caacgaatcg ttggttagcc ttgccgatct tcgcatttgt
     1681 gatgtggctg atctattatc tggctattca aacagtagga acaatgggga cagattggat
     1741 caatgacaca ttgtttggta catggttacc agaacatgtt ggcagattgt tagctgaatg
     1801 gcaagtagcg ggatggatgc aggagctgat cttgaacggc atcatcgcag gcgtgggagc
     1861 agtgttagga tttctaccgc aattgattat attgttttta tgcttgagct ttctggaaga
     1921 ctgcggatat atggcgagga tcgcttttgt catggatcga cttttcagga aattcggtct
     1981 ttcaggaaaa tccttcattc ctatgctgat tgctacagga tgtggagtgc caggcgtcat
     2041 ggccagtcgc acgattgaaa acgagcaaga tcgtcgtttg acgatcatgg tcacaacctt
     2101 catgccttgt tctgcaaagt tgccgattat tgccttagta gcaggggcct tttttcctca
     2161 tcaatcttgg gtagctcctt ctgcctattt tcttggtatg actgcaatta ttttctctgg
     2221 aatcagcttg aaaaaaacac gtttgtttgc tggagataca gctcctttta tcatggaatt
     2281 gcccgcatat cattttcctc aatggtcagc ggtcctaaga caaacctatg accgcagcaa
     2341 gtcttttgtt aaaaaagcag gaacgattat ctttgtttct agtatcgtga tctggttcag
     2401 ctcttcttat aatttttatg ctcaacctgt ttctgaagac caaagtatct tagccttttt
     2461 cgggcgaatc ctggcagttg tttttcagcc gcttggttgg ggaaactggc aaggaacagt
     2521 cgcagcgatt acaggattgg tcgctaaaga aaatattatt ggcacatttg gtattttgtt
     2581 tggtcatgcg gaagtatccg aaaatggcag agaaatctgg caagtgctac aacacacata
     2641 tactcccgca gcagcttatt cattgttagc cttcaattta ctttgtgcgc cttgttttgc
     2701 ggcaattggg gctatccatc gggaaatgaa tgccaagaaa tggacttgga tcgccatagg
     2761 ctatcaatgc ggtttagctt atttagttag ttttgtgatt tatcagttgg gacacttatt
     2821 ggagggtgga caggtagcgt tagggacgta tcttgctatt ttactgatga taggactcgt
     2881 ttatgttttg ttccgtcagc caaaatccaa gaaccagttt tatactatat tgtcattaga
     2941 aggagaggaa taggatggcg acattcttat taagtgtgtt aatctttgga agtgcaggcg
     3001 caattgtcta tactcgttta aaatccggta agaattgtga tgactgtcaa acagcttgcc
     3061 cagtaaaaaa ggaacagatc aagcgatgat cagtcgttgc aagaggctat tctccccttt
     3121 tttgcttatt ttgctagagc atagtaagat gagggaaagt aaaaaaagaa agaggaaaga
     3181 aaatgtctgg aaagaaaaaa atcgtccgcc cgcgctacca gcagattgct gttgatttag
     3241 ccgaacgtat cgttgaaaac cgatataaag tgggtgagaa gatccatgcc cgctcaacac
     3301 ttgccagtac gtacaatgct tctcctgaga ctacccgcaa ggctatcaat gtgctggtag
     3361 atcttggaat catggaagta cggcatggaa gtggggcatt tgttgcttcg aaggaaaaag
     3421 caaaagattt cctattgcaa tatcaagatg tacaatccat ccaagataca aaagcagaaa
     3481 tcatggcaag tgtggagaag caacaagatg agctgaatca tttttctcaa ttattggatc
     3541 agctggtcaa tcaaacgaag cgaatacata agatgaatcc tttacttccg tttgaattat
     3601 ctttatcaga aaaagcaatg catctggacc gttcaattag cgagttgaat atctggcagg
     3661 cgactggcgc cacagttatc gcaatttctc ataatgaaga actgcttgtt tcacctggcc
     3721 cctatgctaa atttacggca ggggacatcg ttcattttat tggaaatgaa tatacactac
     3781 agcgtatgac aaactttttt tacccggagc atcagcttgc agaatgattg tgtgaaatat
     3841 taaaaaaata ttaacttggc taaacctttt atttacaaag atttagccaa gtttttttca
     3901 gtttggacaa gcaaatttct ttcatttttg acaaagtgac tacatgatgt taacatagtg
     3961 tggtcacttt tttcagaaag aaaaatgaat ttacataaaa aaggagaatt tgtttggtaa
     4021 aagtagaagt aaaacattta acaaagatat ttggtaaaaa gacacaagcc gctttagata
     4081 tgatgaatga tcaccagccg aaaacggaga ttttaaagaa aaccggtgca acggttggtg
     4141 tgtatgacgt gaattttgac gtaaaagaag gagaaatctt cgtgatcatg gggctttccg
     4201 ggagtgggaa atccacattg atccgtctat tgaatcgtct gattgagcca acttcgggca
     4261 gtatctatat tgatggagaa gatgtatcga agttaagcaa agaagaattg cgagaagtcc
     4321 gtcgtcataa aatcaatatg gttttccaaa acttcggtct gtttccacat cgaacgatct
     4381 tggaaaatac agaatacggg ttagaagtcc gtggtgtacc taaagaagaa cgacaagaaa
     4441 aagcagaaaa agcattagaa aattctagtt tgctttcatt taaagaccaa tatcctagtc
     4501 agttatctgg tgggatgcaa caacgggttg gcctagccag agctttagca aatgatcctg
     4561 agatcttgct gatggacgaa gcgttctcgg ctcttgatcc gctgatccgg cgagaaatgc
     4621 aagacgaatt actggacctg caagccaatg ttcaaaaaac gattattttc attacccatg
     4681 atttgaatga agctttacgg attggggaca gaatcgcctt gatgaaagac ggcgaaatca
     4741 tgcagatcgg aacaggtgaa gaaatattga ccaatccagc aaatgattat gtacgtgaat
     4801 ttgtggaaga agtcgatcgt tccaaagtgc ttacggctca aaacatcatg gtacctgcat
     4861 tgacgacgaa tatcgaatct gatggaccaa acgttgcatt gacaaggatg cgtaatgaag
     4921 aagtcagcat gctgatggct gttgacagaa agagacacct gaaagggatc atcactgcag
     4981 atcaagcgtt agaagctagg aagcaaaaac gtccattgat cgacttccta gatgaaaatg
     5041 tgacggtgat tgggaaagac atggtggtaa gtgatatttt caatattatt tatgactcac
     5101 cgactccact tgcagttgta gaggatggaa aattaaaagg tgtcgtcatc cgaggtagtg
     5161 tgattgaagc attagcagaa acaagcgagg tgagtgaaca tgaataatct attgaactat
     5221 caattaccag tggcaaactg ggtagaaaat atcacagaat ggtttacgac aactttttct
     5281 ggcctatttt catttttaca aacgattgga caggcagtga tgtcggggat tacaaattta
     5341 ctgttagtga ttccagctcc tttgtttatc ttgttattga cggtagcagc tttctttatt
     5401 tctaagaaac gtccaggact tacgttgttc acattaatcg gtctatggtt tatttataac
     5461 caaggtctat ggaatgattt gatgaatact gtcacacttg ttcttttatc tagtgtgatc
     5521 tcgatcatca ttggggtacc attagggatc ttgatggcaa aaagcagtaa agcccaaagt
     5581 attatcaaac cgatccttga ctttatgcag acaatgccag gattcgttta tttgattcca
     5641 gcagttgcat ttttcggtat cgggatggtt ccaggggtat ttgcctcagt catctttgct
     5701 ctgccaccaa ccgttcgttt tacgaattta gggattcgac aagttcctaa agaattagta
     5761 gaagcttctg attcattcgg gagcacaagt cgtcaaaaac tattcaaact agaattgcct
     5821 ttagctaaaa gtacgatcat ggcagggatc aatcaaacaa caatgctgtc actttcaatg
     5881 gtcgttattg cctctatgat cggtgcaccc ggcttaggac gcggcgtatt atcagcattg
     5941 caaagagcac aagtcgggaa tggatttgtc aacggggttg cattagttat cttagcaatc
     6001 atcattgacc gttttacaca gcatttgaac cagccaaaca ataaaaaagc tgcaggagca
     6061 acaacacaga aaagtaaaaa acgtcaagga ctgatcattg gtgctgtagt agtagtgatc
     6121 ttaggtgcga ttggtatagg gagtttttca tctgccaaag aaacaaaacg tatcaactta
     6181 tcttatgttg aatgggatac agaagttgct tcaacaaatg tagtcggtga agtattgaaa
     6241 caaatgggct atgatgtaac gatgacgcca ttagataatt caatcatgtg gaaatctgtt
     6301 tcaaatggcg agtcagatgc catggtttca gcttggctac ctaaaactca tggttcacaa
     6361 tatgcacaat ataaagatca agtcgaagac ctaggtgcca atttgacagg agcaaaagtt
     6421 ggtttagcag tgccagccta catggatgta aattcgatcg atgagttaac cgatcaggca
     6481 ggtaagaaaa tcataggaat cgagccagga gctggtgtag ttacggctgc tgaaaacacg
     6541 atccaaaaat atgacaactt gaaagactgg aaagtagaga catcttcttc aggagcaatg
     6601 accgttgcac taggtcaagc gatcaaaaaa cacgaaccaa ttgtagttac aggatggaca
     6661 cctcattgga tgtttgccaa atatgatctg aaatacttag aagatcctga aaacggaatg
     6721 ggaagcgaag agcagatcca tacgatggtt cgaaaaggat tgaaagaaga ccagccagaa
     6781 gcttacaaag tattagacaa tttccattgg tcagaaaaag atatggaaaa agttatgttg
     6841 gaaatcaaca atggaaaaga tccacagcaa gcagcaaaag actggatcaa agagaaccaa
     6901 gaattagtcg aaagctggaa aaaataaaaa gacagacacg aaataaatgg agcttccaaa
     6961 attggatcaa atatccaaat ttggaagctc catgttcgtc tctatctttt ttgtctataa
     7021 aaaaagagaa tgcgtagctc atcttcgaaa cgtccaaaga cgccgacaaa ctattatgaa
     7081 tttatgggtg cgagtcaaag agatcgatcg taggtatgaa agactaatgt gattaaagtt
     7141 tattatgtac gaagtgcatt ctcaaaaaat att
//
LOCUS       DACDMM010000133         7050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDMM010000133 DACDMM010000000
VERSION     DACDMM010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.134"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWD16_002210"
     CDS             6..305
                     /locus_tag="IWD16_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9089209.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWD16_002211"
     CDS             378..1739
                     /locus_tag="IWD16_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9089210.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWD16_002212"
     CDS             1761..2021
                     /locus_tag="IWD16_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089211.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWD16_002213"
     CDS             2035..2787
                     /locus_tag="IWD16_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP9089212.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWD16_002214"
     CDS             complement(2881..3486)
                     /locus_tag="IWD16_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9089213.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWD16_002215"
     CDS             complement(3539..4513)
                     /locus_tag="IWD16_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP9089214.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWD16_002216"
     CDS             complement(4891..5670)
                     /locus_tag="IWD16_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9089215.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWD16_002217"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWD16_002217"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9089216.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
BASE COUNT         2157 a         1286 c         1452 g         2155 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa
//
LOCUS       DACDMM010000134         6656 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDMM010000134 DACDMM010000000
VERSION     DACDMM010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.135"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..719
                     /locus_tag="IWD16_002218"
     CDS             <1..719
                     /locus_tag="IWD16_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9089217.1"
                     /translation="DKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEIS
                     EQIRKNMSYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQ
                     HRQIRLYAGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDL
                     SLRHSQYIEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHI
                     LIDDSVSFANEEELIRLFEL"
     gene            complement(792..1439)
                     /locus_tag="IWD16_002219"
     CDS             complement(792..1439)
                     /locus_tag="IWD16_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9089218.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(1447..2133)
                     /locus_tag="IWD16_002220"
     CDS             complement(1447..2133)
                     /locus_tag="IWD16_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9089219.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2139..3212)
                     /locus_tag="IWD16_002221"
     CDS             complement(2139..3212)
                     /locus_tag="IWD16_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9089220.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            3434..4108
                     /locus_tag="IWD16_002222"
     CDS             3434..4108
                     /locus_tag="IWD16_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9089221.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            4113..5453
                     /locus_tag="IWD16_002223"
     CDS             4113..5453
                     /locus_tag="IWD16_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9089222.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(5602..6588)
                     /locus_tag="IWD16_002224"
     CDS             complement(5602..6588)
                     /locus_tag="IWD16_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP9089223.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         2039 a         1263 c         1157 g         2197 t
ORIGIN      
        1 tcgataaaaa agacttctta atcttgaaaa atctcttagg aaaattgaag tacagcagct
       61 atataaaagt agtgaactat tttgtcaata cgattttttc agaattttcg gagaaagaaa
      121 aaaatgaaat cagtgagcag atacgaaaaa atatgtccta ctgttcgttt tcatccgctg
      181 attgcaaaga aaaaaacgaa gaatatgttg ttcaattatt aacgcaaatt tattttttct
      241 cccgtatttt cccatacgat acgactcatt tgacgatccg tgcaacttac tttgcacagc
      301 agatgcaaca ccgccagata cgtctatatg ctggatttcg tgcagaactt caattttatt
      361 ccactctact gaatcagaat ctgatttatt atttatctag ctatatcttt tggatgctta
      421 tcggtaatcc tgaaattcac cattatactt ctgataaaaa aatactgatt atcagcgact
      481 taagtcttcg acatagtcaa tatattgaag aatatatcag tgatattctt gctgtgcata
      541 aaattcactc tgagacaact gctattactg aagatcagct gtcgaaatac aatcttctcg
      601 agtatgatct gatcgttacg aatcaaccca ttttgaatgc ccatgttcct catatattga
      661 ttgatgatag tgtgtcattc gcaaatgaag aagaactcat ccgcttattc gagttatgaa
      721 taatcaaaaa acagctatgt ataaaccacc ctgataaaaa tttcaggaca aatacacata
      781 gctgtttctt tttattgtat tccttcagca atccaacgag atttatagcg aaagtctttt
      841 aacaactctt cttttgtcca attatttgca atgagatccg tcaaagtgtt cattacgagg
      901 gtcatggaaa agtataggaa actaactgct tcttccgacg attgaatgga gaaatgattg
      961 attcttaata attccagcca tttttcgttc attggagaat cagccgatag ttctctacgt
     1021 ttggttgtct gctgatcatt tcgctgggtt aacaagtata acctttgcca atagatactt
     1081 tcgtgatcta agtcgcagca ccactctaaa tatttttcga tccccaaaaa gaaatgttgt
     1141 ttgttttcgc taatgaattt taagatatct tgatggacta tgtgagaact gcttttgatc
     1201 gtatacaaat aagcatcttc caaatcttga aaatatttat aaaaagcacc gcgcgacatc
     1261 cccatcttgt caacgatttc tgagactttg acttggctga tgtgctgtga gcaaaatatc
     1321 tccagtaata ggtcatcgat tctctgtttc tttgcgttcg ataaattgaa aaaagtttct
     1381 tttggcatcc acatctctcc tttatctctg attatacgtg gatagccctt cattgtcatg
     1441 aaggattcat tccttctttt tctcttcttc tgtcaagtac ccatcttcca ttcgataaac
     1501 gcgatcactc cattgaatca tacggacatc atgagtaacc ataatagtcg ctttttgttt
     1561 ctcatgagct tctttaacta gtaatttgac gacttcatat gcatgatctg tatccaaact
     1621 agctgttggt tcgtctgcta agatcaagct tggctcatta tataaagcgc gagcaattgc
     1681 cactctctgt ctttctccac cagaaaggtc tttaggataa ctatttttaa gtgactggat
     1741 atctaaagat tgcagcaagg aatcgatttt tgttgaatct tctttttttg aagtaacttg
     1801 gtcaactagt tcaaattgtt cctttaccgt taaaaaagga atcaaattag agctttggag
     1861 aataaatcct atttctttaa agcgtaaatc tgctcgtttt ttttctggaa gctcagttag
     1921 tgagtgaccg ttgatcacta tttctccgga agtcggtgtt tgcaatcctc ctgaaatggt
     1981 aaggaaagta cttttacctg aaccagaagg accaatgatg ctgacgaatt ctccttcctt
     2041 tacataaaaa tcaattgctt tcaaagctga gacctttgta tgaccagaac caaatgtttt
     2101 ggtaactttt ttcatcgtta atattttttt catttttatt aacctcctat tgctgtaatt
     2161 gggtctactt tggttatcgt acgtatcgaa aacaatccgc ctagcattgc gacaatgatc
     2221 aaaatcccac tatatagcaa ccattgtgac caaaaaacag caaaaggcat cgcgcttggt
     2281 aaaatcagac tagtcaaata agcaaacaaa agagcaagca gaacgcctac caccccaaca
     2341 ataaaggact gtgctactaa agatttagcg atgaagaaat ttttgatccc ttgtgctttc
     2401 attactccaa aaatagcagt cttctgaagt gtcatcacgt acatgaaaat accgaccacg
     2461 gcagcaacca ctaagaacaa aaagtatatc atcgcattta aggtcatatt ttgggcagaa
     2521 tagccaggaa tactttcaat gaatgtccca atagttaata actgtagacc cttcgatgct
     2581 ttttctttcg aaattttagc tgaatccttt attacgatcc cattgatcgg ctgttcttct
     2641 tcagaagcaa acggctgttg tccatacttc aaagcagtcc acgtatctag ggacgcatag
     2701 atgacgggag aaactgtgta ataagtttca ggaaagatac cgacaatcgt caaaggatcg
     2761 tcgtaactgc caattttgat cgtatctcca attttatagc ctgaatcagc cagattttga
     2821 gagatgatga tttcattctt tgttttaaat tcatgccctt tcgtgagttt tggtaataaa
     2881 aatgcttctt tcgttgtgcc gaaaactgta acattttctt tgttttttcc tttgattgct
     2941 ccgctgtaca atccgattgg tgctttctct cctccttcaa tgtatttcag atctcctctt
     3001 gttaattgtg aagctgcaaa tacttgattt gcatcctccg acaaaacaat ctcttgtgcg
     3061 tcccaatcat cgattgcttt tttaaactct tctgaaagtc cattcgctag tcctgacaac
     3121 atgaatacga catacgcaat caagaacata ataccgacga tcaagccata tcgtaattta
     3181 gaatagcgaa tttctttgat agctagaaac atttgttttt cctccctatg atagattgat
     3241 agtgacaaaa tgtcacttgt tgaatagtgt aactctaaag tgacaatttg tcacttgtgt
     3301 tgtttttttc ttatcaataa attgtctaag acatctttcg ataaaacctt aacatgagtc
     3361 ataaaaaatt tatttaagcc ttatttatgc tttccatgat aaactaaagt gaccaaatca
     3421 atgaggtggg aaaatgatta aactattagt agtagaagac gataaagtat tatccaataa
     3481 tataaaagaa atactcgctg caatcggcga gataacacaa gtttatgatg gtagtgaagg
     3541 actgttcgag ttggaaagtg gagtttatga tcttgcaatc ttggatttga tgatgccgga
     3601 aatgaatgga tatgaagtgt tagcaaaaat acggaaagaa aaaatacaga ctccagtatt
     3661 gattttgact gcaaaagata gtttggaaga taagattgaa ggatttgaac gaggtgcaga
     3721 tgattatcta acaaaacctt tctatcgaga agaactgatc atgcgcgtga aagcattatt
     3781 gaaacgctcg ctaggtctct ttgatgaaaa tgttctgaag tataaggata tcagttgtga
     3841 tttaaaaagc aatgaagtct tgtataaaga acaagttttg cccatacaag gaaaagagtt
     3901 tgaattgctc gtttattttt tacaaaataa gggaatcatt ttgacaaaag agcagatatt
     3961 tgaccggatc tggggatttg attctgatac gacgattaca gttattgaag tttatatgag
     4021 caacttacga aaacatctgc gaagtactgg attggataaa gaaatcaaaa ccttacgtaa
     4081 tgtcggttat atactgcaaa gggtgtgatg caatgaatgg agatattaca cgaaagcaac
     4141 gtagtcgctt ttttgcgaca aatgtagcag catttgcttt tatctttttt cttttgggat
     4201 tcattacttt acagatatta aaccaatctg catacagaga aacagatctg tcattagaga
     4261 aaataaattt tgattctcga acagtacaat tagagataca aagatatcag caaggcgatc
     4321 cctttttaaa cccgactcaa acggaacaaa acagaacaaa catggaacag ccattaatgg
     4381 aaccatcaaa tgaccgtttt aacactcaag tgatcttgtg gtctaaaaat ggtgagatat
     4441 tgaataaagc agctattggt gggcgtttca accaaatcaa agaattaaaa atggatacga
     4501 aaaatctgaa taccatccaa aatattgaat taaatgaaga cagtggctca tctgccctct
     4561 ctttccgttc gattacgaaa aaagcggaga cgaatcaaga tggcgttgcc tacatccaaa
     4621 ttttagaaaa tacaaatcaa atcaagaatt cattagcgac ttttcgcacg attatgattt
     4681 tgtgtatgat tgttttttgg attctctcta ttggtatcag ttattacttg tctagtctaa
     4741 gcatgcgccc tattattctt tcttggagaa gacaaaaaga atttgttgag aatgcttctc
     4801 atgaattgcg cacaccgtta acgattattc aaaatagttt ggaacatctt tttaccaagc
     4861 ctaatcatac tattattgaa gaatccgaaa gcatcgctca agcattgtca gagacgaggc
     4921 gcctcactgg attgacttcg gatttgctga caatcgctcg gaatgattct gatcagcagc
     4981 tgctttcaaa gcaaatgatc aatacacaag aatatatcga acaacttgtt aaaccatttc
     5041 aagagatggc catcatagat ggaaaagagt ttattctcca taacgatgca acattagaag
     5101 taccaattga tgaaaaaaaa atccatcaag ttttggttat tttgctggat aatgcattaa
     5161 aatatacgaa tccagaagat aagatcattt tggattcatc tgttaaccat aaagaatggc
     5221 tgattcacgt aaagaatact ggcccttcta tcagtgaaga agatcaagca aggatttttg
     5281 atcgatttta tcgggaagat caatctcggg cgaaagagac tggcggatac gggcttggct
     5341 tggcaattgc aaaacaggta atcgaacagc atcatggaca gatttctgta tccaattatc
     5401 ttccacaagg agtcgatttt aaagtccaat tgccgttgaa gcctaaaaaa taatgcttat
     5461 ccctaaaaaa gggctgtgac aaaaatcttg tcacagccct tttttgttgc gaataaacgg
     5521 tgttccagaa gtagcttctt cggaaataag ccgaaatttc tttaagctga ccacttctgt
     5581 cacaacctct ttagcttacg cttacgattt tttattgcgg tcttcaatgt gtacatcacc
     5641 gcttgtgtca atatccaatt cttctcggct aacatcttct gtcacttttt caacatcttc
     5701 atgttcttct ttatgaatcc caacttcttc acgaataact gtatgtttgc ttacatctac
     5761 ttgctcttct ttgattggga tagtaatcgt gtcatcttcc aatttttcgt ctgttccctg
     5821 tgaagattga tctgttactg gtttgcgttc gatgacgatt tcttcacgtt tgaccggtac
     5881 ttctaccgtt tcagtatcgt tgaccacatg tttgtggatg tcgacttctc cagttgtcac
     5941 atcatgtgta tttacatcta atttttcttc ttttagttga attgtatcat catctagatt
     6001 tggtttgcga tcgatgtctt cagtcttgct tgtacccatt gtatcagtct cgccaggtac
     6061 gtccatcgga tcagttcctg gtgtcatatt caatggatcc gttgtatttt gaaagcctgt
     6121 cgtgccacct acgtccattg gatctgttcc agtgccagtt tctaatccag tccccattgt
     6181 gcttgctggg ctaacagtat cttgtgtatc cattgtatct tctgttgttt ctggctgacg
     6241 atagcctttt actgtgatga caaattttcc atcagaaata tctcctctat attgatataa
     6301 aggatcattt tctggtgtag ccgtctcaga atcataggta tcaaaagaaa aagtatcttt
     6361 tattttttcc cataaagaac ggtcttcttc accatccatc cgattttcat ctgtttcaac
     6421 atctatacca gacaatccgt caaatcctag tctagatgct gcttcatcag tagtataaat
     6481 aacgatatca tctcgttcat acccttcgtt acgcaaccgt tcaattactg ataatgcttc
     6541 ttccctcgta ctatagcttc cgatgaccgt taaatttttc tcttccatgt aaattcctcc
     6601 tagcaaatag atttatgatt ggtatattgt tacttataaa tttattctat aggaga
//
LOCUS       DACDMM010000135         6517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDMM010000135 DACDMM010000000
VERSION     DACDMM010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.136"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            2..1012
                     /locus_tag="IWD16_002225"
     CDS             2..1012
                     /locus_tag="IWD16_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9089224.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1081..2025)
                     /locus_tag="IWD16_002226"
     CDS             complement(1081..2025)
                     /locus_tag="IWD16_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9089225.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2009..4729)
                     /locus_tag="IWD16_002227"
     CDS             complement(2009..4729)
                     /locus_tag="IWD16_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9089226.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4726..5946)
                     /locus_tag="IWD16_002228"
     CDS             complement(4726..5946)
                     /locus_tag="IWD16_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP9089227.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(6227..>6517)
                     /locus_tag="IWD16_002229"
     CDS             complement(6227..>6517)
                     /locus_tag="IWD16_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP9089228.1"
                     /translation="MLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAI
                     GDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
BASE COUNT         1902 a         1259 c         1066 g         2290 t
ORIGIN      
        1 aatgaaaaaa aaatcaatta ttttggcagc tactagtgcg ttggctgttt taactctagc
       61 agcttgttca ggcgatacta ataaagatat cgcaacgatg aaaggcggaa cgatcactgt
      121 atctgatttc tatgatgaag caaaacttga atcatcgaac caatccttag tacaacggat
      181 gatcatttat aaagtattca ataacaaata tggcgataaa gtaaccgata agcaagtgga
      241 tgcagaatac gacaaacaag cgaaatcatt aggtgatact tttgaatcac aattagaagc
      301 tgctggatat acgaaagata cttataaaga atatattcgt aacaatttag cttttgaagc
      361 tggcttgaaa gctcacgtag atattacaga tgacgatttg aaaactgctt ggaaatcttt
      421 ccatccagaa gtagaagcac aaatcatcaa attatcaagt gaagatgaag caaaagatgt
      481 gaaaaaatca gcggatgatg gagatgattt ctctaaatta gccaaagaca aatcaactga
      541 tacagagaca aaagaagatg gcggtaaagt taaattcgac tcaacgacaa caactattcc
      601 tgcagaagta aaagaagcag ctttcaaatt gaaagacggt gagatctccg atgttattac
      661 tacaacaaat cctacttctt atgcgacaga atactacgtt gtcaaaatgg tgaaaaacca
      721 aaataaaggc aatgacatgg ataaatacaa agatcaacta aaagatatcg ctactgaaac
      781 taaactaagt gacaatgcat tcacaactaa agtaatcggt gaagaattaa aagatgcaaa
      841 tgtaaaaatc aaagatgatg cctttgaaaa tgtgttgagt gcattcacaa caacttcatc
      901 aagtacgaaa gactcaagtg aaactacagc ttcaacaaag agttctgaca caaaatcaac
      961 tgattcaaca aaagaatctt caactgaaga aacaactgat tcatcaaaat aaaagaaaac
     1021 aaaaaaagcg aagacttatg tctcgctttt tttgttttga atgaaaaaaa taaatttttg
     1081 ctataaatct tttggtacat agaaacttcg gttgtccata ccgaaaatcc gatcggtgta
     1141 ttttcctggt tctgtttgac gaatcgaagt caacatcatc tgcatcgaag catcgatatt
     1201 gtcgatttga tgtaatattt ctgcttccat tattcttgga cgtacaggtg agccatattc
     1261 taataagcca tggtgtgcta aaaccatatg acgtaataca agaacatctt cgtcagcttc
     1321 gctgattttc aacgcaagac aggctttagt tatttcttcg tcaatcaata ccagatgacc
     1381 gatcaaattt cctgcaagcg tatattctgt cgacatagga ccggaaagtt caatcacctt
     1441 tcctaaatca tgcaaaatga taccagcata caacaatgct cgattcagct caggatattc
     1501 atcggcgatt gatttcccta agtgaagcat tgtcaaagta tgataagcta atccactggc
     1561 aaatgcatga tggtttcttt ttgctgccgg aaactcaaaa aattgttttt gatatttatt
     1621 aagtagatag cgaacaatac gattccaatg cgcattggtg atctcaaaca acaattgatt
     1681 gatttcttct tccatttctt ctttcttcaa aggggctctt tccatgtata aactaggctc
     1741 tcctggctca tctacccttg ttaagcgcat atgaagaatc tttacttgag ggtttccttg
     1801 ataaacctct cttttaccgt ttagaaaaac gactgtgccg gctgtaaatt tattgatttc
     1861 ctcctccgaa gcatcccagt atttaccatc gatagtacct gaagtatctt ggaaagtaaa
     1921 agcaataaat ttttttccgt ttttagcgat tcttacatct gcacttttga tcaggacaaa
     1981 ttgttcaaac tgctcatcga ttactaaatc acgaattttt ttcattattt gctccttcta
     2041 attgttttac gatttggttc atctcatggt aatagcttac catttcttta tcagaagaaa
     2101 aacaaattat ttgatacttt tctccaaatt tagctaaaag ttcagctaat tgatactttc
     2161 gctgatgatc ataatgaagc cagccatcat caatgatgat cgggcatatc gaacggttag
     2221 cttctaatga gaggtaagca aatctcatcg ccataatcag ctgatctttc gtacctgtag
     2281 ataactcata gatgggaaat tctgtatatc cgtctgttaa agtcagtgta tcatttttca
     2341 gtaatatctt ctgataattt ccttttgtta aaatacctaa ataatcacct gctgctctta
     2401 acaactgggg taattgtctt tcactcatct ctgttgaaag atcacctaag aatgtcccgg
     2461 cgatttcaag tgctccccat tcctccagta attcctgtaa ttttgctttt tgccggctag
     2521 cttgttgata aagttcgtcc aatgtgccgt ctctttgcaa ctcttctact tgaagttgga
     2581 gtcgttgccg attttcactt gcttcttttt cttcttgctg gagttgggat tgcttttctt
     2641 tcagttggcg cagtcttaca gataatttct cttttgtcag ctcttcagga aatagtggag
     2701 taagcatttc cgataattca ttgtatcttt tcatctgctg ttcggcttgt ttggcttttt
     2761 gtaaaaagac aggtacttcc gaaggataag ataagcctgc ttgagtcaat tgttcgctgt
     2821 attcttgtat ccatactttt tgtttctttt tcaattgatt gatttcttgc ttcaataaag
     2881 tattttgctg atagcttctg gcaaatttca tctgttccat ctcttgtata aaacgggaaa
     2941 gcaataccat tttttctgaa attgtcttat cttgtatcgg taaccattct ttaacaaact
     3001 caaatttttg gtcgatttgt cgtagttttt cttgtatttc ctgttctctt ttatgtttcc
     3061 gctcaaatac gccatataat ttctgataat ctatgacttc ctgtccatat tctttcatga
     3121 gtttgagcgt atccatcttt cctaagtgcc gatgatccgc aaaacgatat acttcttgct
     3181 gaagaatctg tttttcttct ccagttctct tttgtttttc ttctgcttca gctaattgag
     3241 ctaaggctag atcaagttgt cctagttttt cttgccacat tccttttatc tcagagaaat
     3301 catttttagt tttaattttt ggttgccttt tgtagaagaa atagccaatc atgcctgcac
     3361 caattagtaa aaggatcact ttcaagggca ttggtacaaa tatacctgca atgattattg
     3421 ctccgccagc catcagcata tacggtttag atgaagagac tggtgctatc tctgactgat
     3481 tttgtaataa tgctggatag gtctcttcca agtcattcac ttctgcttca atcatctctt
     3541 gctgttcttt agctatttgg agacgcacag aaatctggct ttctagatca gcaatccttc
     3601 tttcttcagc cagaagatgc ttccattctt cttcatataa aaattcttcc ggtaacggat
     3661 ttttctgcca atgccacctt tgttccaaaa tcttcaattc tgaatttgtt tctctagtct
     3721 caatttgtaa tctttgccat tcttcgttta attgttccgc tgtaaattga agttgctgga
     3781 gatcttttag ttcctgctcc tgctctaaat aaaaataata tctggctgac tgctgatcca
     3841 tcccgctatg cttttccagt gttgcttcta attcttccaa acgctcattt gtttgctgat
     3901 accgtttgga gaaagactcc agatctaaaa gaagttgctc atcaggaaga cctgtagtgc
     3961 tgtttttttt gagagattgc cattcttcat ataaagaaaa attaagttct tgctgtctgg
     4021 ctaatgaaaa ttgtttgttc gcctctttta attgtgccaa taactgctgc tgttgcgact
     4081 cgtattcctg ttcttgttgg atcaacttgc caaaataagc ttcctgttct tgtttttgat
     4141 gaattttttc ttgcagctct ttccattcat ttaatttttg attgattagt aatttttggc
     4201 cttttttctt gaataattgt tgtgctttct gataatattc ttgccgcttt tggaatactt
     4261 gagaactgcc cgtgatgcct aaagaaatca gcgcatcatg tagctctttt tctctcaaat
     4321 gatctagttc catcaactgc tcttgctgga aagtaaatac ctgctggaat aactctttcg
     4381 tcaaaggtgc gatcagtttt tctagtaact catcacttcc cacttgatcc cccagccaca
     4441 ccttcgattt tccgcgattt tgctgcttgt agcgctcgac agcaaattca ccatatacag
     4501 gatgtttaag ccatattttg ccgccatagg ctgagccgtc ttttggtgta taatcctttt
     4561 ttttacggcc ttttgaaggg aaaccaaata atattgcttg aataaattga taaatcgttg
     4621 attttcctgc ttcgtttccc ccatagatca gctgattgcc tgaataaaag tcaaaacttt
     4681 tttgacgcca ttttccaaaa gcagcaatct cgatccttaa aatcttcatt cctgatcctc
     4741 ctcaaataaa aaatcttgat taagcagctg cttggctttt tcaagtgttt cttcttgata
     4801 ttccggcagt tcgctcaata ttctagccgc ttcttgatga ctatatactt catttaaaat
     4861 ttgttggaaa tcttgaggtg tctgatagta ttgaaataac tgttccatca atttagcaga
     4921 agcagcaagt ggggtcttcg tataaaaagt attctctatg agagaaatac gccaaataaa
     4981 gaaatcattt atcctatctg agaattcctc taacaaatag tctaatagct ctcctgattg
     5041 tatccgttcc aaaacatctg tattgagatg gtcataatct tttaatttta tctcaattaa
     5101 actaaatcca tcctctactt ttgaaagctg ctgacgaatg tgaacaatga cctcttttgt
     5161 cgttcttgct ttcctcaatg atatttcctt tgtcttccaa tagacctctg ctactttaat
     5221 cggatgtatt tggcaacttc ctttacttaa ttcaactagt agaatagatg tagaagcttg
     5281 ttccttttta gtatgacctt gaggcgcacc tggataaaca attgtttgct tttcatttaa
     5341 gaccgttggc acatggatat gccctaaagc ccaataatcg tatccctttt cttgcatctt
     5401 tgagggttgg aaaggagcat agtttccttt ttgagagacg ccaggttccc cgtggtacaa
     5461 cccaatatgg taatctgtga gttctctaga aggaaattcc atcactttgt ctttttgaat
     5521 ccacggttga cgataactaa atccactgag agagacagtt tctccacttt ttatcgttat
     5581 tttttttgtc tctaccgttt cggatgtaaa taaatggaca tttttaggaa aggagaacca
     5641 ataccgctct ttttgataga aatcatgatt tccgaatatt ataaatacag gtatatggtt
     5701 cttttctaac cgtttcatct gttctgaaaa atgtttctgt atttttaatg aaggacgatt
     5761 ttgatgaaaa ttatctcctg caagtaaaac aaaatcaacg ttatttatta ttgcctgttc
     5821 aatgatattt gataaaacgg tcagattcgt cttcaatagt ttttcctgta cagttttatc
     5881 aagagttgtc agtccttcaa atgaccgatc catatgtaaa tctgccgcat ggataaattt
     5941 caccattaaa aatttcctcc tttttttctt ttatcatacc attttcaatg ttttttttca
     6001 tcaataaccg aaaatttgtt cgtattatgc aaagaaatta cgataaatgg ccgacttcaa
     6061 aaaataagtt tattcgaaaa aaagattgtg acatgatttt tgtcacaatc ctaaatatct
     6121 atgttatatt cgcttcgttt gacactgaag aagtactatt tgtactggaa tttatgctcc
     6181 atttttccgt atttagaatt ctatgttcta tactacctta attagtttat atggtaagaa
     6241 ttctttcaaa tgcttgagat actccgtctt tatcatttga aacagtgaca taatcagctt
     6301 ttttcatgat ttcttgatga ctatttccca ttgcaaaact aatatctgca ctttcaaaca
     6361 tagataagtc atttaaacta tctccgatcg cgattctcaa aggtttcttt aaattgtaga
     6421 ttgattccaa acgatcaaac accacacctt tagatacatt ttgaggtacg atttcaatat
     6481 ttacaggtgc cgattcagta acagacacat ttagcat
//
LOCUS       DACDMM010000136         6470 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDMM010000136 DACDMM010000000
VERSION     DACDMM010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.137"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(37..459)
                     /locus_tag="IWD16_002230"
     CDS             complement(37..459)
                     /locus_tag="IWD16_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089229.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(522..767)
                     /locus_tag="IWD16_002231"
     CDS             complement(522..767)
                     /locus_tag="IWD16_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089230.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            912..1751
                     /locus_tag="IWD16_002232"
     CDS             912..1751
                     /locus_tag="IWD16_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9089231.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            1902..4919
                     /locus_tag="IWD16_002233"
     CDS             1902..4919
                     /locus_tag="IWD16_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9089232.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            4983..6446
                     /locus_tag="IWD16_002234"
     CDS             4983..6446
                     /locus_tag="IWD16_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9089233.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
BASE COUNT         2421 a         1110 c         1327 g         1612 t
ORIGIN      
        1 aataacaaga gggtttttcc tcttgctatt agaaacttat ttcagtgagt aagcaaagta
       61 ttgttcattt actttagcca aaccaaacgt atccccaatc tttaacttgt taggggtata
      121 ttgaatgtca ggaatggaga ataaattttc tttggtattt gttactttga tagaaaattc
      181 aaggtcagtt ccttctttag gagtaataaa cgcaatattt ttcttttcaa ataagacagt
      241 agtaatttga gaagaaccaa gatcaattaa atcttgtcca tgcgtttctt ttaaactatt
      301 tagtgaagga tagacggaat gatcgctaga ttgacttgta gtagtcgatt gaaccgtatt
      361 ttgtggctgt gtttctgttt tgttggatac agaaaaagca ataatagcga gtacaatcag
      421 tacgattgaa ccaactgaaa caatgtattt cttattcata gaacctccgt tgtaaaagtg
      481 tttattattg ttttacttgt aaatccaaga cagaaaacgc ctcattgacg tttctgtctt
      541 ttttcttcga gctgttttgc gtatttttca atatcttctc ttagatataa gcgaatgatg
      601 gaaggaccag gttcgtcgcc tatttctaga aatggtttga tattatttct acgtaaagag
      661 ctagaaaaag catcatagtt ttgtccagtg attttggcag cttgtttacg attaagtaaa
      721 ttattttgag cccaatcatg tatttcattt aaattattaa gttgcatgtg tgacattcct
      781 tttagttatt ttagtaccaa agttagtata tcaaaaaata aaaaaatcta caaaaaatat
      841 atcgactgat agacataagt ttggtactat gtttttgtaa ataaatttat tcaaatcgga
      901 ggcgaaaaac aatgaaaatg ttgaaagtat tggcactagg aatcgtagtc ggaggagtta
      961 gtcttgctgt gggaacgaat gctgaagcag cagaaaacaa agtttggaca cctcgttcgg
     1021 cggaacaaat caaagcagaa attaaaggga atgaatacac aattgtctgg ggagataccc
     1081 taagcgcaat cagtcaagca acaaatatta ctgtccaaaa attagcggac atgaataaga
     1141 ttacaaatgt ggatttaatc tatgcaggaa ataaattagt atttaatggc aatgtagcaa
     1201 gtgtacaaaa cagcgcaggt gaaactgtgg ctcaaacagt gattcaagag aaagacaaag
     1261 ttgatccgaa tcaaccagta ggacaaggaa cgacttcaaa tacacaaacg acaccaaata
     1321 caacgacacc aacaacacca gctggagatc aaacaggaac aactggtggt acaacaacgc
     1381 caagtacacc agaaaacaat ggtggcacta ctcaaacacc tacacaacca ggtaatggtg
     1441 gaaatactgg aggagaaaca actccttcta acccaagcac aggtggagga acaacaacgc
     1501 ctgaagaacc aagcaatcct ggtggaggcg atcaaggcgg aaatggtggc acaacgacac
     1561 ctgaagagcc aacaattgta gttggagaga ttggtaatag tggaaaggta tttaacactg
     1621 tagatgaagc ctatgattgg gctaatgcag aggttagcga tccaaattcg cagtggtaca
     1681 acaaaggttt tatggtggat gttattattt acagtgacgg gtcacaaaaa cacactgtag
     1741 atttctacta aaaataaaag aaaagtaagt ggttgatgaa gactagaatt ataagttcta
     1801 gtcttttttt gttttgagga ggataaaaat gtttcggtgt cgaagtccat aagaaattac
     1861 tttagaagac tgaaaaataa aatgaaagga acgaaaaaaa tatgtttaaa agaatggcaa
     1921 cttggttggc aattttcgtt acatttctca atcccatgtt aggatctgta acggcatttg
     1981 cagaaacgat aaatgaatca aattctactg aagaagtaat gaagactgaa gaatcaaaaa
     2041 gtagtgaaga agcgatcagt actgaaacca caagtgaaac aacagaagag acaactacgc
     2101 aggacacgac agaagatgtt tctccgccca atgtaatgac tcaagaggtc cctgaaggtt
     2161 atgagaaata ctttataacc attgaagata ttcaaaaagc gacttctttt tctgaacaag
     2221 aacgattggc gcaagaatct gaagaatggc aagcaatctt ggaaaagaat gcacagacac
     2281 gtgctaccaa tctaacattg actgaaaatg gtcgtaaaga tgtgaatatg actcgaatta
     2341 ctcctgaagg aacaatgtat actcatgatt acatggttcg ctggcaaatt gacggagaag
     2401 atgttttttg cattcaagaa ggcgcaatca cgagtgcggg tattcaatat gatgtgacac
     2461 cactcgaaaa tttaattacg acaagtgcta ttagaaaaaa gatttcttta attggttatt
     2521 ttggctacta ttctagtggt tcaatggata actatgtatt agcacaaaca tatatttggg
     2581 aagttttagg tggttcattt gttaattatg gaactattgg acaatctaat tatgaagctt
     2641 tcaaacagac tgtagataga aaaataaaca atttacttga agatccttct tttgatagcc
     2701 aaacagtgac ggttaaagta ggggaagaag tagaagttaa ggatacaaaa ggtgtgtttt
     2761 ctcaatggga tgggtcggtt gttgaaaaca cagctaacgt tcaagtaaga aaatcaggaa
     2821 atagtttgtt tataaaagca aatcaaaact caaatgacgg acgaatttct gtttcttcac
     2881 tggatccaaa ttctagaggg gtaggttcaa gttttgccta tgtagatcct gttgctcaaa
     2941 atctaggaaa gctttatgtg ccagatccta atagagctgg actaactatt aaagttctaa
     3001 aaaatggcga cttcaaacta accaaactag acgctgtcac agggaaaaaa gttccaaatg
     3061 tgacgtatga aatcaaacgt catgatacgg gagaaacaaa acaatataca acggatgcca
     3121 aaggggaaat cgccttaaaa gaaatccctg cattctatga aaaagacgga aaatatgttc
     3181 aaaataaatt ggatgtcaaa gaagtcaaag caccagctgg gtatattcta gacaaacaaa
     3241 ctcatacgat tacgattgaa ccaaacaaag aagtgacgtt aactgcaaaa aatgacttac
     3301 aacagttcat tccagaagga aagaaagtca aagaagtcta taatgcggaa gaatcctgga
     3361 atcaaggcaa acctgtttat gataccgttc ctgcagaaaa tatcgaattt gatattatcg
     3421 ccaaaacaga tattactgcg ccagataaag aaacagtcct tgtgaaaaaa ggagaagtct
     3481 tggattcagt aaaaactgac aaagacggac actggaaggc aaacaaaaaa ttctacaatg
     3541 gcgatcaaaa caaataccaa ttgattgaaa aaaatgtgcc agacaactac cgtcaacctt
     3601 ctgaagaaca aaccacgttc tctgtggcct atggcgataa cacgacgcct gtgcttacgt
     3661 ttgaattaga tgtgattgac aacttgttaa aaacgggtga agtagacttc aataaagtcg
     3721 attcaatcaa tcatctacaa ttaccaggag cgaaattcct gattgaaggt atgactgcac
     3781 ataataaaaa tgtgtccttt gtgtatgaaa caaaagaaga accagaaaaa ctaaaattac
     3841 cagacggtac gtataaattt acggaaattg tgttccctga gcattatggg caagcgtcag
     3901 gacaaacaga aacgatttta gtaacggtca aagacggaga agtacaacca attactgtga
     3961 aaaacgaaga aatcgtggtt ccagaagaag accaaccaga aatttctact ttgtttgtga
     4021 caacgacagg tggaaaagat ttcaacccga aaaaagacca agaattagtg gataaagtgt
     4081 cccaagtatt ccctaaatct gcggttggga cagagaaata ttgggtgacg caattccata
     4141 aaattactgc cgatggaaaa gtcactgtat tagatacggt aaaagaaaca cgaaaagtga
     4201 ccgatgaaaa agaaacattt gatgtgacgt tcaactacaa agcagacatg gtttcagacg
     4261 gtgacacgat tgtcgcaacg cacattgtct ataacgacaa agaagaggaa gaagaattcg
     4321 ctcgtcattt tgatttaacc aataaagatc aaacagtgac tgcgaaagaa ccaaaaatcg
     4381 aaacacgctt tattacagtg aatggcgacc aaacatttga tccagacaaa gaccaagagt
     4441 tgatcgatca tgtatcgcaa gaatttccag aatctacggt aggggaaacc aaatactggg
     4501 tgactcaatt ccataagatt gataaaaatg gaaaagatac agtcgttggt acggtagaaa
     4561 ctgcacgtaa agtttctaaa gcgtctgaag agtttaaagt atcctttgac tacaaagcag
     4621 gcacgttaaa agacggggag aaattggtcg caactcatat tgtctataag gataaagacc
     4681 ataaagagga agaagcacgt cactttgatt tgaagaatga aaaacaaaca ttgacagcaa
     4741 aaggcattaa aaaagaaacg ccgacaaccc ctgtcaaaca aacgccgacg agcacaccaa
     4801 aacaaagctt accaaaaaca ggagaaacag tgaacgatgt gttaattgca ttgggactgt
     4861 ttctttttgt tggtgtgggg gctgttctgg ttcgtcgtaa aatgaaacaa agcaattaaa
     4921 gtatgactag agacagcaat cagctgtctc tagatgattg attgaagaat ggaggcaatc
     4981 ttgtgaaaaa aacgagtaaa tgtttatctg ctttgttatt agtaggtcaa gtatttgggt
     5041 atacgccagc ggttttggct aatgaacaag gcaacaatca actgacaaat tcgcaatctg
     5101 caacgatcac tgaagaaacg acaacaaatt ccactggaga agaaaatcaa ccagaaacac
     5161 cgactgaaga tacaatagat acaccagata atccagaaat gaacgctcca ccaacaggtt
     5221 ctgaagaaca accagaaaca ccgaatcaac cagacaaacc agaagacaat caacaacctg
     5281 aggaggaacc gtcagaagaa ccaaatgagc aaccttctga tgaaagctca cagactcctc
     5341 aaaaaccaag taccgaaaca gaagcggaac cgccaacaaa aacggaacgc ccacaaaagg
     5401 cacctgaaca gagtgtgact gtacctatga atgacgtacc gcaggcacaa tcaatcggtt
     5461 cagaagagac aaaaaactac caccaagcac caagcgcacc gatacaagaa ttagtccctt
     5521 ccagcacaca agataacgaa gggactattc attttgaaaa agacgcgtct gtagaaacgt
     5581 ttatccgtaa aatcggtgaa tctgctcgta aaattggaca agaaaatgat ttatacgctt
     5641 ctgttatgat cgcgcaagcc attttagaat ccgcaagcgg acaaagtcaa ttggctcagg
     5701 caccaaacta caatctattt ggaatcaaag gcacacataa cggaaagggc gtctcttttg
     5761 cgacacaaga agatttaggc aatgggacac tttatacgac ccaagcaact tttcggcaat
     5821 atgagagcta tgaagaaagc ttaaaagact atgccaaact gttaaaagaa ggcttagttg
     5881 gaaatacctc tttttatgat ggtgtttgga aaagtaaggc aaaaacctat caggaagcga
     5941 ctaaattttt aacaggaaga tacgcaacag acactagcta cgataaaaaa ttaaatgggt
     6001 taatcgaaac ctatgactta atcaaatatg ataaagaagt agctggtcca caactaagca
     6061 aagaaggcta tatggtacct ctaaaaaatt attcaatttc tagttccttc ggttggcgag
     6121 gagaagaatt tcatagagga ttagacatgg cagccaatca aggagaacca atctatgcga
     6181 gcaaggcagg aacggtcata gaagcttcgt atcatacttt gtcaggaaat tatgtcacaa
     6241 ttgagcatga agacggaagt gtggctcttt atgcgcacca acaggaatat tgcgtgaacg
     6301 taggacaaac agttgaacaa ggacaaatta ttggttatgt gggttcaaca ggaaatagta
     6361 caggaccaca tttacattta gaacttgcct tagatggtag tttaacaaaa gacaaattaa
     6421 tcaacccaga aacagtctta ttctaaagaa taaggctgtt tctgataagg
//
LOCUS       DACDMM010000137         6469 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDMM010000137 DACDMM010000000
VERSION     DACDMM010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.138"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..362)
                     /locus_tag="IWD16_002235"
     CDS             complement(<1..362)
                     /locus_tag="IWD16_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9089234.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYIL"
     gene            complement(491..1138)
                     /locus_tag="IWD16_002236"
     CDS             complement(491..1138)
                     /locus_tag="IWD16_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9089235.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(1222..2649)
                     /gene="uxaC"
                     /locus_tag="IWD16_002237"
     CDS             complement(1222..2649)
                     /gene="uxaC"
                     /locus_tag="IWD16_002237"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9089236.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(2723..3802)
                     /gene="uxuA"
                     /locus_tag="IWD16_002238"
     CDS             complement(2723..3802)
                     /gene="uxuA"
                     /locus_tag="IWD16_002238"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9089237.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(3805..5436)
                     /locus_tag="IWD16_002239"
     CDS             complement(3805..5436)
                     /locus_tag="IWD16_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9089238.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            5753..6424
                     /locus_tag="IWD16_002240"
     CDS             5753..6424
                     /locus_tag="IWD16_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9089239.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
BASE COUNT         2044 a         1292 c          891 g         2242 t
ORIGIN      
        1 catagtatat atgttacata catatatata actttaatac tcatagaagc tgatgccatt
       61 gatgtttgat acattaaaaa acttgctaaa gcgactggac cactcatccg taaaatccac
      121 ggtttaaatt tgccagtttt agatggtttt gatccatcca caatacgccc cattgttatg
      181 tctgtaactg catcaataaa tcttgctact aaaaataatg ttcctacggt agctccgctt
      241 atccccaata cttttgtata aaaaatcatt aaaaaagaac tagcaaaaat gaaagtgaaa
      301 tcattcccaa aatctccaaa catatatcct attttgtctc ttagaccaaa ttttctttcc
      361 atataatttg cgctccttcc tattgttaag cgttttcatt ctgttgaact gccaacttac
      421 gtactatact ctgaagtata aagagttagt atttattttt tacattttat agtattattt
      481 attttttctc ctaataattc tttatctcat aaaacttatc tatatattta ctagctattc
      541 gagttacttc ttcaaaatca cctttttcaa caggagccaa caaattccca cctacaccaa
      601 ctgcaacaac gcctgcttca aaccactctt ctaagttatc tagacttact ccgccagtag
      661 gcattatatt tacatatggt aatggagctt gatacgtttt tacaacagat ggaccaaaag
      721 cagaacctgg aaatagttta atgatttcta ctccagcggt aagtgcctcc tttatctcag
      781 taattgtcat acaacctggt aaataaggaa tttgatataa attacaaatc tctgctgttt
      841 gtcgatcaaa agatggactt acgatatatt ctgctccagc cataattgca atacgagcag
      901 taaacgcatc taaaactgta ccggctccaa ttattacatc tttttgatca gcatattttt
      961 tagcaatttc tttgattatt tcttcagcat tcggaactgt aaaagttagt tcaatccctt
     1021 taattccacc cgcagtaatt gcatttattg ctttaatggc ctcatcttta ttttttccac
     1081 gtacaaccgc aatgactcca gccgcttcta acttacttaa aatttctact tttttcatat
     1141 ctatctcctt cttttatgga tacatactct agctagttgt actaacttac aataaataaa
     1201 tttaagcttt tattttttgt attattttct actttcgatt tcaaaaaaat taaaataatt
     1261 atacgcatta cgataacaaa tattctccac tatctgacta agatacattt catcatctgg
     1321 aattcgacct gctttaaccc actcgcttag taattgacat aatactcgac ggaaatactc
     1381 atggcgagga tagcttagaa aacttcgaga atcagtcaac atacctgtaa aattcgacaa
     1441 caaactttgt tgcgccatcg tagtcaactg ttgacgcatt ccttcagctg tatcattgaa
     1501 ccaccaagca catcccaatt ggattttttg aatcataccc ccttggaagc tttgcattgc
     1561 tgttgctagt ggaagccaat cggttggatt taatgaatat aaaattgttt ttggtaagca
     1621 cccactcttt tcagcagtgt cgaacaatgc tacaacattt tgtgtcaaac tagcttgatc
     1681 ccccatcgaa tcaccaccga catctactcc aatttgttcc tttaatatac ttgaattatt
     1741 tcttaaagca tttaaatgca tttgcatcgt ccaaccatat cttgtatact cagacatcaa
     1801 atgtaattga atacctgtaa caaattgtgc aatttctgat aaatggaaat ccgtttttcc
     1861 tgctaaagct tttattaaca gattgtcgag tattttttca tctgctattt cataaaaata
     1921 agtattcaaa ccgtgatctg ctaatcgaca gccaatttga tgaaaataat ccactcgttg
     1981 gctcaatgcc tgcttcaacc cattaaagtt tttgattggt atacccgctg cttgactaag
     2041 ttttaaaata tagttcgaaa atcctgatga tttgatactc cagaccttat ctggacggaa
     2101 agtaggtaat accttgaaag aattatcctg tttagaaagc tcagtatgat atcgcaagtc
     2161 atctgtagga tcatccgttg tacaaatggc tttcacatcc atcatcttca gcaattcttt
     2221 tgggcgatag gaatccgttt ggattttttc gttggcacgt cgccaaattt ctggtgcatt
     2281 cttctcagaa attgtcaaat caatatcaaa gtaacgttgt aattccaaat gagaccattc
     2341 aaaaatagga ttacctggcg ccttctccac tgtttttaca aaagctaaaa atttttcata
     2401 atcattacct gcaccactga tcaactcttc ttctatccca ttagctcgca tcaatcgcca
     2461 tttataatga tctccagctc ctttatcatt taaccatacc tgagtcaaat tcttgtattg
     2521 cttattttct agcaaacatg ctgggtctaa atgacaatga taatcgatga ttggttggtg
     2581 ttctgcaaat ttatgaaaaa gtcttttagc tatttcattc tctaataaaa agttcttatc
     2641 taaaaacata tttaagcact cctttccttc ccttagtttt tagaagagaa aatttttctt
     2701 ctaaatagac tccattatat aattatttac tcgccccctt ttcaagcgct tcccaaatac
     2761 catttaaata aactgcacct agagctcggt catataaccc ataaccaggt ctaccggttt
     2821 ctccccaaat catccgacca tgatctggac gaatatagcc attaaatcca ttatcgtaca
     2881 ttgtttttaa tatcttatac atatctagag aaccatattc tgatttatgc ggcgcttcat
     2941 aaaaatcgag tccattatca cttaaatatt tgatattacg aacatgtgca aaaggaatcc
     3001 gatcccgttg acaaaattca gatagaattt catagacatc attatctggt cgttctgcaa
     3061 tacttccagt acataacgta ataccattag aaggagaatc cactacatta cacaaccatt
     3121 ctaaatcgtc acgattttta acaatacgag gtaatccgaa aatagaatat ggtggatcat
     3181 ctggatgaac tgccatttta atcccaactt cttcacatgt cgggataatt gctttgagaa
     3241 aatatgcaaa gttctctcgc aatttcgctt catcaacttc tttataggct tctaataatt
     3301 gcttgatttg tgataaccga tgaggttccc aacccggcaa ttcaaaatta ttagtagaat
     3361 tttctacttt tctaataatt tcctctggat cagtaggaat attttttgaa atataggcta
     3421 acgtagaaga cccatcatct aaatgataat ccaaatctga ttttacccaa tcaaagactg
     3481 gcataaaatt ataacagata acctcaatcc caaattctgc caaattgcga atcgtctgtt
     3541 tataattctc gatgtattga tctcgtgttg gcaaaccaat tttgatatca tcatgaacat
     3601 taacactctc aataactttc aaggtaaggc cattggcttc gacttcatct tttaaagctt
     3661 taattcgttc tctcggccag acctctccgg ctggtatatc cattaatgtt ccaacaattc
     3721 ctttcattgc tgggatctga cgaatttctt gtagtgagat tttttcttca tttgccccat
     3781 accatctaaa agtcatttcc atagttactt tcctccaact tctgttatat atttattgat
     3841 tgttttacga actgcttgag gacctactag catttcgcaa aagatttttt caattttctt
     3901 tcctatctca acttgatata aatctaaacc aaatatttcc ttattttcta agatttcctg
     3961 taccgcacaa tgaatctttg caggatcttg ttcacctaat ttcaatactg agaatttttc
     4021 ctgcaactct accaataaag gatcaggact tggcacaaaa ttttttcctt gatcatcgac
     4081 tgccaataaa taacgtaacc aagcagcaat agcgatagga ataaatgtta gattttctgc
     4141 atttccgtct ttttcgacat actttttaat tgtttcacca tagcgaatcg ctactttttg
     4201 agaagtatct gttgcaattc gttgcggtgt atctggaata tagggattag gcaaacgttt
     4261 attgattact tcatcaataa attgttttgg atcaataacg cctggacttt caactactgg
     4321 taagccttct acataaccta tccctttaat taagccaacc aaatcatcat cttgcatttc
     4381 tgcctgaatt gatttatacc ccaacaaaca acctgttatt gctaatgccg tatggagcgg
     4441 atttaaacat gtcgtaacct tcatgatatc tgctttatca actgtttctc tatctgtcat
     4501 aatcacacca gccgcttcca gtttaggtcg accatttgga aaactatctt caatgactag
     4561 ataatggact ttttcagtat tggtaaaagc agcgatattg gtatttttcg cagtatgaat
     4621 tatatctaag tcttcgaaac ccgccttcaa taaatccatt ttgacacttt ccgaaggatt
     4681 tggcgtaatt ctatcaatca tactccaagg aaaagtcact ttttctttat cctttaaata
     4741 agcaataaat ttttcagaaa cgtatccttt tttttgccaa gcttctgcaa tcataaaaat
     4801 gctatcttga aaacgttgac cattctgtga gaaattatca gtagaaacca tcgcaatcgg
     4861 taactctcca gcattaaacc tgctatacaa caaggatgtc acaatactca tcgtatgttg
     4921 aggattggta ggtccagatt ttatatctgc ttccactacc gaaaaaaatt gtcctttaga
     4981 atcttttaaa ctgtatcctt tttcagtaat agtaaacgtt gtaaactgta atgtaggatc
     5041 ttcaaaatat ttaaccactc tttgatagtc cgttatattc tttggattgc aatataaact
     5101 atcagctgtt actgctaaaa gtcttttttt gagttcacca tcttcaccca taacaatctc
     5161 caaaatatca ttgttgtagt catgataagc tttttctact acttcttcat caaatgtctc
     5221 acacacaact actccggatt ctaattcatt ttgatctgct aagcattgag caatttctgc
     5281 atgaaatcct cgataaagat ttcctccacc aaaatgaagc cacactggat gtttctgacc
     5341 ttcttgtttg accgcttcga cattaaaata cggtagttga acttgcattt tgttcaatcc
     5401 ttcgatagaa tcttgccagt ctttaatcaa aaccatattt acccctccat actgatatgt
     5461 tagtttccgt tacaattgta gtataaaatc gaatgtaatc gctgtcaata agaaaaaact
     5521 aaaaatattc ttttttctac catgtcagaa ttattgtgtc tttttttagt aaaagcacac
     5581 caatatatat tttttagaac taacttaaat taaagcttaa tttctctttg atcttatttg
     5641 agtatttttt gtttttttaa tagctagtat gttactttga aatttgatat tatataacga
     5701 gcatagaact aaaatatcaa attcaaaaat gaaattttga gagggattaa atatgacaat
     5761 taaaactagt ctccaaaatc aagcttatca atctattcgt cagcaaatta tctacgctga
     5821 cttagaacct ggaaaaaaag tatcagaaaa aaaattggaa gaaacgcttt ctatcggtcg
     5881 tactccaata cgtgaagcat tgattcaact ccgtcatcaa ggacttgttg atacaattcc
     5941 tcaaagtgga aattatatct cattaatcga cttagattta gcaaaaaatg ctcgttttgt
     6001 cagagaacat ttagaacgac aaattatgct agagtgttgt gccaagatga ataatcaaaa
     6061 gaaacaagtt ttagaaacta taattgcaga acaagaaaaa gctgttagtc aaaagaatgc
     6121 tcgtagtttt tttcataatg ataatttatt tcataaagct tgttttgaaa ttgcagaacg
     6181 ggaagaagta tggcaatggt tggaagataa taatacgcac ttggaacgat ttcgttggtt
     6241 acgagtaaaa acggaaggat taaaatggga aaaaattatg gaacaacatc atcaactatt
     6301 taaagccttg accatgcaag accctgaaga agctaacttc ttaacatcct tgcatcttca
     6361 tttaatgctt gatgaacaaa atgttgtatt gactactcac ccaaaatatt ttaaaaatat
     6421 ataactgcta aatttctttt ttttaattta tttacatttc tgattacta
//
LOCUS       DACDMM010000138         6404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDMM010000138 DACDMM010000000
VERSION     DACDMM010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.139"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(580..1143)
                     /locus_tag="IWD16_002241"
     CDS             complement(580..1143)
                     /locus_tag="IWD16_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9089240.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(1158..2201)
                     /locus_tag="IWD16_002242"
     CDS             complement(1158..2201)
                     /locus_tag="IWD16_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089241.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(2334..2738)
                     /locus_tag="IWD16_002243"
     CDS             complement(2334..2738)
                     /locus_tag="IWD16_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9089242.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(2971..3606)
                     /locus_tag="IWD16_002244"
     CDS             complement(2971..3606)
                     /locus_tag="IWD16_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9089243.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(3872..4924)
                     /locus_tag="IWD16_002245"
     CDS             complement(3872..4924)
                     /locus_tag="IWD16_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9089244.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(4937..5875)
                     /locus_tag="IWD16_002246"
     CDS             complement(4937..5875)
                     /locus_tag="IWD16_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9089245.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
BASE COUNT         2024 a         1323 c         1008 g         2049 t
ORIGIN      
        1 tattattttt tttctttact tgcattactt aattatatac tcctttcatg gattgcttta
       61 tctagtataa cttaaatttt aaataaatcg ttatttattt ttactctcac ttaaaaaagc
      121 actaaaatta taagagaggg aggtaatggt ctgaacacca agaattgttc caacatcgaa
      181 ttgcttaact aatgagttca aagcttaatt atgtggctct tatatttacc gagcgtggat
      241 tgcgatctct ttagcgaaac aaacatcttt cataaaaaaa acagccatat ccgctttctt
      301 ggatatgact atttactact actttactat ctttcattct tcttgtctca cttatgataa
      361 ggcgctgcgt atcatgagtg aatgatatta ggaaaacttc ataaaataaa tcttttatac
      421 atttccactt ctagatggtc attcatgtta gaagaatcaa actatttttt tactgtcgta
      481 ataatattaa aacactagaa caaatcaatt aaattccata aaatataaat aatagaccta
      541 gcatcatctg gtataaaatg gatgctaggt ccgttatatt tactcaaaat tgattctacg
      601 aatagatctt cttatcattt ctattttagt agtcatgcgt tgttgttctt ctaaaggtgg
      661 taatggtata agtagtttag ataaatttgt accatttaca tttggttgcc cagttccgta
      721 agaaattttt tctaataact tccaatataa tggcgagtct aagaagcagt ctacgtattc
      781 tgataataat ttctcaacta acctaattcg aatcaagtaa gaagcaaaaa cagattcctc
      841 acttatttcc ttaaccaaga aactttttcc catagttcct cctgtccttg ctatcaaaat
      901 gtcattttct tccaatctta aatcaactag tttagaatta ctaatatcac agtaaggcac
      961 actggaccaa tttacacgtc cctcttgtat atctgtgatt ctcaaaaact taacattgcc
     1021 ttgttttttc gccgaatctg tatagccata ttgaatctta gtagaaatac ttcctaaata
     1081 aacccatagt cgaacatcgc tagatgattt ttcttgtcgt tctttatgaa caacagtgat
     1141 catcttgagt tgtccgacta ttttttagtt gcagcaatct ttttgggacg cttatttgca
     1201 tgaagcaacg ttgctaagtc tagtaataaa acattggcta acacaggatt ggctttctcg
     1261 cgatacgctg cggcgccact ttcaaacaat actgctagac gatcttctac aacaacgatt
     1321 tgttccaaat acggtggaaa tttgattcgt tttaggatcc ggctaggaga gtcccgctcg
     1381 gacactaaga gttccgaggt ataaggacca taagaacgac tggctaagag ataatggcca
     1441 taaaaagcca caccttgaat cttatcagat gtttttctgc tcattgtcac ggttggttgg
     1501 attttgtctt tgcggcgaat tgctgttttc atttcagcta tcgtcggcaa tagatagaaa
     1561 gtcaactgtc cttcaccttt tagtgtgaag ttgccagata ccagttgatc attgttttga
     1621 gtcaaatacg atgcttgtgg aatctctgag agatcagttg tcagccaata agcgattggc
     1681 tgtcccgtct cttcagatgt atctgctaag atatcgatca aacgtaaagc ggctactcgt
     1741 ccagtagcag atcctgtgat tgtcaaccat aaaagttccc gttttgtatc atatactaag
     1801 ccccctacat gtggccgttt aggtaaaatc agcagtttga tcggctgtcc tgtttctcta
     1861 tccagtacat gaataataga atgatgctga tggtcatagc agtaggcaga aataaacaca
     1921 aaatcttgag aaaaagccaa cccttgcggc gtcatccatt gacaaacctc attttcgccg
     1981 gtttccggca ccaccattct tgtttctgtt aatccaggaa tgttatattg ttttgcttca
     2041 gccttttccc aagctgcttg aacaatagga taccgtttta actttttttc aatatcctga
     2101 tgtatccgtt gctgcttcca atgcttaaac tttaggacat agtaaacaag aaggccaatg
     2161 accagcaaca ctaaaatcat tattgctacc cgctgtgtca tttttttgcc tccttgttct
     2221 attcgatcaa tcgtccatat accgctatta attaccgtaa ctacatgttt cttttactag
     2281 cgtttcttta taggatcttt cataatctca tttatagtaa cattaagaat cccttattct
     2341 actatagtca cttccacatc aatattgcct cttgttgctt tagagtatgg gcaaaatgcg
     2401 tgggcttctt ctgcgatttt ctctgcttgg tccttctcaa gtcctggcaa agaaacttct
     2461 aaaacgaccc cgatttggta gcccccatct tctaattgaa ataaagatac ccgtgctttg
     2521 acttcagaat cagactcaag attattttct tccagcatgt gttgaaccgc gccgttgaag
     2581 caagatgcgt atccagccgc aaaaagttgt tccggatttg tcccttcact atggattcct
     2641 gggcggtcga tcttcatgtg catacttccg tttggtgcct caacttcgcc atcacggcct
     2701 ccacgattga tcattgctgt ttcgtacatt tttttcattg agatcatcct ttctttcgtc
     2761 gttctatatt aagtgtaacg agtatttttt aatctgacaa aggtaatgct tgcattcggc
     2821 gacatcagtt agacaacatc gatgatcagt gatagaagca gtttatctat acaaaaaaag
     2881 aagggttcta tgatatttgg tgtccttact cgccttgagt aacaacacca aatatcatag
     2941 aaccacttaa tcagcttcac actgcttttt ctactttcgt agttgaaaaa aacttaaaca
     3001 aatcatcggc aaatagttaa agaaaaaact taatcgttga aagaagccca attgcttaaa
     3061 gaaggcaaac tgactaatcg acggaatccg tgctaatccg taaagacctg caattagtaa
     3121 actaataact gttaaaacaa gataaaaatg gctaagcgta ccttgaccac tttgacgata
     3181 aagcagaaca agtaacaatg ggaaaagtag aaagcctgca tagcctagcc cagaacctat
     3241 attatggatc caagtcgata gattacaaga gctttcattc gtattgatac tgaataagcc
     3301 agtaaaaata caatcaccta tcccatataa accaactgtt gcaacaacaa gtattgcaag
     3361 tgtttttgat gttccaacaa ataggtgata caacgcaggc aacgaaagaa caaaaaaaag
     3421 tccagataca actgaccaga ccaagaaagc acgtctgact ggactatcta cgtcaccaaa
     3481 cacactaatt acggtagtca tttgattcag tttgggataa aaaaggccaa ggatatacgg
     3541 tgtcgataaa tcactcatca cacctaataa taaaaaataa aaaccatatt tttgcaaaaa
     3601 aatcattttc ttcccctact ttttagtgat tattcgatac atccaacaat caaatttttt
     3661 ctcattttaa cacatatttt tattctagtt gtcttttgaa caatcaataa cataaaggtg
     3721 agtttcttct acattttaaa aaatcgaatg gcactgaaga gttcagttgt cagcatcaat
     3781 ctcctactca aaaaggcaaa aaaacgtaag tcagatgttg ttgacatctc acttacgcat
     3841 ccattgattg aaaatttctt agtaaaatct actagcctaa aaatttgaag acttcttctc
     3901 gatcatacag tttaaatact ttctcgcata aaaattgagt aattagtgcc gcaaaaacag
     3961 ggatgactaa ccaacaaaga acaacaagaa agccatttag ccctgcatct aaagaagcca
     4021 acggaccaac aagtccgacc acgccgaaac cagcagaagc aggcgtacct gaaatagaaa
     4081 atagagcgac aggaatagct gtaatcactg cactaactaa acaaggaata agaatcacag
     4141 gttttctaaa tagattaggc atcatcatct tcatagcgcc taaagcaatc gcaattgtca
     4201 cacctgactt attaaccttc caagagttca ctactaaaac aaaagtggtt gcagctacgc
     4261 ccatcgcagc cgcaccagca gaaacaccat ctagttggat tgccattccg attgctactg
     4321 ttgaaatagg agagataatc aaaaaggcaa aggaacaacc aatcaagata gacattaaca
     4381 gcggttgtaa ggtagtgaag ttattaatca atgatccaat tgcagaagta attgctgtta
     4441 caaaagggaa taaataaaaa ccaagtaaac ctgcacctac acctacaaca atcggtgtta
     4501 acacaatcga cactgaccca aaactatttc caatcagtaa aatcagcccg acagcaatcg
     4561 atgctgtcag catcgtatta ataatatcgc ctgtaccagc accaacgtaa acacccgttt
     4621 cacccaaatc aggtgtgaat ttgactacac cagaaccaac aaaagaagct ccggctacaa
     4681 ccatcattct catcggatca aatccaaatt gtaaggcaat caaaccacca atgatcaaag
     4741 gtgttgctag ttgaaaaata actgctaact gtgcgatcgt taccgcaaag ggatacgcac
     4801 taaaatattt taaaatggca cctaatactg cattaggaat caatccaata atgattcctg
     4861 ttgctgtacc agcgaggact ttattcagaa aaattttcgg tgatattttt tgtgaagttt
     4921 ccattcaatc tctcctttat tttacgttca aactatcttc ttttgcatgg acaagctcag
     4981 tcaagaaatc acaataaggc gtcgcaacac catatttctt cccttttcta gaaattgcgc
     5041 cattgatata atcgatttct gttaatcgat ggttcttaat caagtcttga tacattgaag
     5101 gataatgtaa cccaattgtt tccgggtcaa aacaagattc acaatgtgca atgacttctg
     5161 ggacatctag ttcaatcttc tctactgctg ctactttggc aaactcattg acaatcgtcg
     5221 ccaccatttt atgagcagtc gatgtttttc ctagctcagc catattaacg tctaagattg
     5281 tgcatagtcc gttcattgtt ccgttcacac atgctttgcg ataaatagaa tagtgaatat
     5341 tgtcagaaaa atgagcattt aatcctgatt cagacaattt gtctgctaat ttttttgcag
     5401 ctgcttcttt tccatcacct aaattttgta attctactga accacttcca aataatttga
     5461 cctgaccagg accttcaaga cctgccgtcc acatggtatt tccaatatag atattttcca
     5521 ttggtacaaa tttctcaata atatcttcat gcccgatacc atttaataga cataagacct
     5581 ctgtatcttt tttgattaat gattggatat cctgcagcat tttttccagc tgcatcgctt
     5641 tggtaaatag aataatcaga tcaacttgat cttctttttc tacttcggat tgaagaacga
     5701 ttggtagttt tgcttctacc tctttaccat taaaatttgc ttgtaatcca tgttctttga
     5761 tttgttggac atgttctgcc cagccatcga ttaataatac ttcgttccca ctttggtgaa
     5821 gcatcaaccc aaagcgactt cccattgcgc cagcgcctgc aattgctatt ttcattgatt
     5881 cttcctcctt tttctttcta ttatactcat ttgagaatct tttcacaacc tcgttttgtt
     5941 attcttctct tatttatcat gtttatatcc accattcaag ttattttatc agcaaatcaa
     6001 taaaaactgg aatagaatcc caatttcatc gtaagagaaa ctagactagc aataagaaaa
     6061 tagaaacatt catagaaaaa atctcactcc tctgattata aaagaggggg cattaacctc
     6121 gtgtatacta aaaccttcca ttcattcgat ggatgatttt ctttttgtga aaaaatattc
     6181 attgtagtac gatgttgcgc aacacagacg gatggtattg cttattattt attaaaatcg
     6241 aacaatacac ttatttaaaa acttctttcc cagattttct tccattttta cagttataac
     6301 tagtattatc atgaaaaata gccttttatt gtctatggga ataataattt aaaattggta
     6361 cttttttatt aattagttca tcgcccaata atctctccag tttc
//
LOCUS       DACDMM010000139         6352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDMM010000139 DACDMM010000000
VERSION     DACDMM010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.140"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..185)
                     /locus_tag="IWD16_002247"
     CDS             complement(<1..185)
                     /locus_tag="IWD16_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP9089246.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFP"
     gene            409..1209
                     /locus_tag="IWD16_002248"
     CDS             409..1209
                     /locus_tag="IWD16_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9089247.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            1228..3096
                     /locus_tag="IWD16_002249"
     CDS             1228..3096
                     /locus_tag="IWD16_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9089248.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            3089..3580
                     /locus_tag="IWD16_002250"
     CDS             3089..3580
                     /locus_tag="IWD16_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP9089249.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3568..4122
                     /gene="srlA"
                     /locus_tag="IWD16_002251"
     CDS             3568..4122
                     /gene="srlA"
                     /locus_tag="IWD16_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP9089250.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            4140..5135
                     /locus_tag="IWD16_002252"
     CDS             4140..5135
                     /locus_tag="IWD16_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP9089251.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(5277..>6352)
                     /locus_tag="IWD16_002253"
     CDS             complement(5277..>6352)
                     /locus_tag="IWD16_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9089252.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         2140 a         1028 c         1299 g         1885 t
ORIGIN      
        1 attgggaaaa atttcaagca gtagatcgca caaataccta cgacgatata aatgatcttc
       61 gcaaaaatag ttgttgaacc accagcaatc atagctacta aatcaagttc gaacaagcct
      121 accaacagcc agttcaaccc tcccacaatc aataaagtta atgcaatagc atctaaagtt
      181 ttcattagaa ttctcctttc ttaaatgata aaacaattat atatcaaaaa gagaaacatt
      241 gcttatataa cgctcaggaa ttttagcaat ctatgcaaaa agatgaaaat tttttttgct
      301 cttccattag tacacatttt ttagctgaag ctgtaaattt catgtcttaa ttttttttag
      361 tgagctttta tactggattt gtaagaaaaa agaaggagag gatcacatat gtcggattgg
      421 ttggatattg caggaaaagt agtaattgta actggaggtt cctcaggaat tggaagaagt
      481 atcgtcgaaa atttgttgaa gcagaatgca caagtagcga attttgatgt aactgaatgc
      541 cgtatacagc atgaaaacct actatcttta aaagtcgatg tgtcttctaa aacggacata
      601 aaagaaggga tatataaagt aatgaaacat tttaagacga tcgatggtct agtcaataat
      661 gcaggaataa atattccttc tcttctaata gatagaaatc atccaaaaag caagtatgaa
      721 ttatcagaac aagtttttga taagatgatc gctgtcaatc aaaaaagtgt atatttgatg
      781 agccaagcag ttggaagaat cttggtgcaa aagggatcag gagtgatcgt gaatctttct
      841 tctgaatcag gattggaagg atcagaagga cagagctgtt atgctgctac aaaagcagcg
      901 atgaacagtt ttactcgttc ttgggcaaaa gagttaggaa agaggaatgt tcgtgtggta
      961 ggtgttgcac cagggatact ggaagaaacc ggtttgagaa cacaagaata tgaagaagct
     1021 ctttcttata cgagaggtat aagcgtcgaa cagctgagaa acggttacag tcgaacaagt
     1081 acgatccctc taggaaggag tggtaagctg caagaagtgg cggatctagt ctgctattat
     1141 ctttcagaaa gatcaagtta tatcactgga gtcacgacta atatttcagg aggtaaaaca
     1201 agaggataaa ggataggggg gacgcacgtg acgctagtaa acagatggta ccaattattg
     1261 aaattactag tcactcacaa gaaagtttca atccaggaag tgcaaaaaga aatcaaaacc
     1321 agtaatcaaa ccataaaaaa agatattggg gagttgaatg aagaaattgc cggtattgca
     1381 aaaatcatcc aaaaaaacaa acattttgaa cttgaaattt atgattttga tttttttgat
     1441 gagatcatgc aaggaaaatt gaaaacagcc agtgatttca attcggcatc caaaagaatc
     1501 gcttatttac taaataggct gatcgaaaca gaggatttct tacgtatgga tgatttgtct
     1561 gaagaagtcg gtgtcagcag aggaacagtg gtcaaggatc taaagacatt aaaagagatg
     1621 atcaatgatt ttgatgtaaa gattacaggc acaccaaata aaggactaag aataatagga
     1681 gatgagctag aattacgact acttttattt tattttgttt ctccctactt ttcagagacg
     1741 aaaacagaac agtttctaga agaaaaacaa gcaatcaaac attttcagaa gaagacgaat
     1801 gctagtaaac aagaaatcac ccttttaacg aaagttatcg gtatttcttt gaatagaatc
     1861 tctagtaact atctgttgaa gaagcccatc aatcgttaca gtgggtgttt tgaatatatc
     1921 caagaattca atgaactgat caatcagttg gaagaaatct atgaactaac tttgagccaa
     1981 tatgaaaaag attttatcag ttttcctttg aacatcatca acacaagtat aacgatgata
     2041 aggaaagcta actctgattt gcgcttatat ttcgacccca tgatgatgcg tattcgacga
     2101 ttagatctca ttgatctgga tgagtccttc ttatatcaag agatgaaaca gcacctctct
     2161 ttattgataa atcgggtaat ttttcgttat cgattgactg atttatttta tggtgagata
     2221 gaaaaaaagt atcctttttc ttaccaaatc gctagcgaat gcatacaagc attagaagct
     2281 atcttgcaac ataagatctc taaagtagag atcagctacc taacgttgta tttggagtta
     2341 gcatttcgat atcaaaaaga gcatttttcc tctaaaaaag tagccattgt tcatacgaca
     2401 ggaaaaggaa cagcattaat gatccaaaga caaatcaaaa ggatattggg gagggatata
     2461 caaataacac atgtttcaga agaacaatat gaaaaagtag atctaaacaa atatttcgct
     2521 gtttttacaa cgattccgtt aaaaaatgta aatgaagaga caccggtgat ccatttgact
     2581 aatctttttg atgactcttg gttaagggca caatggcaaa aagcaaaaaa aaagcgatta
     2641 gccgggtttc attctactgt ttttttctat caaatatgga aagaaaaaaa gacgtatcaa
     2701 gaatatttaa acaaattagt cgctcctttg atcagaggct attatgtgga tgaagaattt
     2761 gaaaaaagga tcttgaaaag agaatatcag caaagcacat ttgttaacaa agaatttgct
     2821 tttcctcaca caaccaacca gctatctcaa cacattgttt tatcagtagg gctaataact
     2881 gatcaaggtt tagcagaaac aggaggaaat ggagttattt ttttatttgc catacctgaa
     2941 gaaatgacgg ctgaagttga gaatgaatta tttgagctat acgatatcct atttgaactc
     3001 ataaaaatgg gaatagatga gtttattgaa ttagcaccga cacaagaatc atttttaaaa
     3061 ctaatcaaaa gtaaaggaga gagacttcat gaatgatatg tatctattca tagttttcgc
     3121 agcttcagct tatgtgctgc aagcagttat cagttggaag caactaaagc atttcaatca
     3181 aacatatagc gaactgcgaa gaaaaggaaa agtagcgatt ggtaaacgac ctgggaaaat
     3241 ccgttcaggc acgattgtta tgctagctat agacgatcat gatcaagttt tagaagtcag
     3301 aaaaatgcaa ggggttacat ctttggcaaa atttcattca ttacctgtct atgcgggtga
     3361 agatatccat tacatcgatc gttaccatcc ctcagttcga gcggaagatc gtttgacaca
     3421 agcagcttta gaaaatgcca gagaagtata cttacgaata gaggcaggaa atgaaaaaag
     3481 tgaacaacct tcattgcctt ttattgctat gctcaataat tttcggatat ggaaaagtac
     3541 gatcaaagaa aaaaggagtg acaaacgatg gatatcataa caaaatttgc tgagggattt
     3601 atgaggctgt tccaaacagg tgcagaaaca ttcataagct ggatgacagg aatcgtacca
     3661 gttgtcttga tgctgatggt tgcaatgaat acgctgattg cttttttagg tgaagaccga
     3721 gtaacgaaag tagccaaatt atcagcgaaa aatccgctta ctcgttacct tgtgttaccg
     3781 ttcttatctg cttttatgtt gggaaatcct atgtcattta caatggctcg tttccttcca
     3841 gaatactaca aaccaagcta ttatgctgct caagctcaat tttgtcatac gagcaatggt
     3901 gtattccccc atattaatcc tggagaattg tttgtatgga tggggattgc tcaagggatc
     3961 gaaaccttag ggctaaattc gatggagctg gctatccgat acttgcttgt gggactgctg
     4021 atgaacttta ttggtggttg gataactgat tttactactg gatttgtatg cagacagcaa
     4081 ggaatcgttc ttagcaaaaa agtagagctt tcagttgatt agagaaagga gagttttaaa
     4141 tgtatggtga tattaagatc gtaagaggaa acggcgggta tggaggtcct ttgattatta
     4201 ggccgacaaa agataaacac aagttcatct atgtcacagg tggaggtgag aggccaatga
     4261 tcgtagataa aatagaagaa ctgacaggta tggagtctat caatgggttc aaaacatcaa
     4321 tacctgatag tgaaatcgcg cttgcaatcg ttgactgtgg agggactctt agatgtggaa
     4381 tttatcctaa aaaagggata ccaaccatca atatcgttcc cacaggaaaa agtggcccat
     4441 tagcacaata tatcactgaa gatatttatg tttcagcagt agggttagaa caaatacagc
     4501 cactaatgga aactgatgat aacaaagaaa cagtaaatac tgcatctcca gataaaaagt
     4561 acaaatatga tacttctaaa aagataaccg agcaaaaagc agagcagcaa aactggatag
     4621 caaaaattgg aatgggcgcg ggaaaagtag ttgctacatt caatcaatca gcaagagaag
     4681 cagtagatac gatgatccgg acagtgatcc cttttatggc tttcgtttcc cttttgattg
     4741 gtgtgataca aggttctggg atcggtaatg tatttgctaa cattatggca cctttagcag
     4801 gaaatgcagt tggattaaca atcattggct ttatttgttc actgccattt ttatctcctt
     4861 tgcttggtcc aggggcagtt attagccaag tgattggaac attgatcggt gtagagattg
     4921 ggaaaggaaa tattcctccc caattagcat taccggcact atttgctatc aatactcaaa
     4981 atgcatgtga ttttattcca gtcggtctag gtttggaaga agcaaaagct gagacagtag
     5041 aagtaggtgt accatcagtt ctttattcaa gatttcttaa cggcatccca cgagttatca
     5101 ttgcttgggt agcaagtttt ggattatata gctgagttta gtctaatggt gataagtcaa
     5161 gatgaaattt aagattttct ttattgagta atatgttttg aagcgcacga aactagaccc
     5221 gatcaaaatc aaattttttt cgggttgatt caaggatttg cgtcaataac ggattgttat
     5281 ttctctatca cactccttgg tggcgtatag agttggtgtt tacgtagtag cgtatccacc
     5341 agacgcgtaa attttcttgc ggttaggacg agtgcacgtt tgtgttgatg ctttggaacc
     5401 tccttatatt tactttgata aaaagctttg tattcaggaa gataattttt tacagagttg
     5461 gcagcttcaa ctaagtagta tctaaaatat cgatttcctt gttttgtcag aggtgtattt
     5521 tgcgactgat agtttccaga ttggttcact ttccaactta atccagcgta ttttgccaat
     5581 tttgtctgat cttcaaatct ttcgatttga ccaatttcag cgattaaacc ggcagcatag
     5641 actttaccaa cgcctggaat actggttaag cattgatatt ctggaatgac aacaattact
     5701 tgttcgatgg ctttatctaa ttctttgata tttttttcaa gtgctcgtat ttctcgtacg
     5761 agaacactta aaacaacatt aattgattct tgtgcaagag cacctaggcg gtaactcatg
     5821 gtaattgcac gttgaattgc tttagcgatt ttttctggtt gcttgaaacg gccttttcct
     5881 ttttcttgaa gtaaatcaca gaaagcttct aaaggaagct cagcaagttc atccaatgtg
     5941 aaatcttccg tcatgagtga aatgagggtt gcactaaaaa gactcgtcga ttcgtctcgc
     6001 atttctcgag caagcgtatt acatttgtac gttagatttt caagaaaatg ttgctttgta
     6061 cgaatcaatt gcttaataag ttgatagcga gtacgtgtta aacgctgtaa ggccatgtat
     6121 ttctcttctt tgatagggga agttgtgaat cgttgaatac gcaagaaatc agcgattctt
     6181 aatgcatcgt ttcgatcagt tttattctca tcaaacatgc gactaaattg tttcacccga
     6241 aaaggatttt caatcgttac aagtgtgttg agcttcttta gtttctcatc ttcatgaaaa
     6301 aacatggaag gatggaagct gtacatggac gttgactcca tgccaatcac aa
//
LOCUS       DACDMM010000140         6319 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDMM010000140 DACDMM010000000
VERSION     DACDMM010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.141"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..2510
                     /locus_tag="IWD16_002254"
     CDS             <1..2510
                     /locus_tag="IWD16_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP9089253.1"
                     /translation="LSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKR
                     KEGSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETIL
                     TIQNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSI
                     PDNIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYT
                     IKEGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVK
                     KYPLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIE
                     QKVITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRE
                     NAPIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVH
                     KYVLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLF
                     GNPTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLR
                     LDSNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKV
                     KSLSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALI
                     YSGALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKF
                     LTNSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSG
                     NMCAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2692..2931
                     /locus_tag="IWD16_002255"
     CDS             2692..2931
                     /locus_tag="IWD16_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089254.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            2947..4320
                     /locus_tag="IWD16_002256"
     CDS             2947..4320
                     /locus_tag="IWD16_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9089255.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4324..5958
                     /locus_tag="IWD16_002257"
     CDS             4324..5958
                     /locus_tag="IWD16_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9089256.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6121..>6319
                     /locus_tag="IWD16_002258"
     CDS             6121..>6319
                     /locus_tag="IWD16_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089257.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2211 a         1033 c         1282 g         1793 t
ORIGIN      
        1 aattgtctga aggtacagta aacataactt ataatcctat taaacaagag gtgattacac
       61 aaaaaaagca ttctgtgaga gacaatgata ttgaaaaaat tcggtttgta tttattgata
      121 ttgaccctaa aaggaaagag ggaagtgcaa cagatagcga aaagaaaaag gcagaaaatg
      181 ttatggaaca agtagaacgt tatttgaaag aaaagagaat agaatcattt gtaaaagttg
      241 atagtgggaa tggctaccat atttttcttc ccataaatga acaaccaaat aatcacgaga
      301 cgattcttac tattcagaat tttcttcaat tacttcaccg aagatttgga gtagaagatg
      361 aagttgatat tgatacttct gtttataatc cttcaagatt atgcaaatta gttggtacac
      421 cagcggtcaa aggaaagtcc acaaaagagc gtccgcatcg tttatctagt ttcctttcta
      481 ttcccgataa catagataga agtaattgtt ttaaaattct tgaacagata gttgaagaaa
      541 attccatgat gtcaacctct agagtaaaaa gccatccatc ttttagagaa gtgttacctt
      601 ttattcaagc agactataaa caatggttag ctcactatcc tgaattaact tataccataa
      661 aagagggcga tcataaagga gttaccttac ttatatttga cgaatgtcca ttaagaaaac
      721 acacgaataa ttctaatggt tcttgtttgt ctgttacggc tgataataag attcgatttc
      781 attgtctgca tgcttcggat aaaaataaag atattcaaga ctttgtaaaa aaatatccgt
      841 tgccaaaacc tgtaaatcct tctgtagaat cattagaatc caatcaagaa tattattttg
      901 accagtttaa actgaatcaa gaaggagtat ttttggtgaa agaaggtcaa gaagaaaaag
      961 ttgcttgtcc gatgtttatt aagcaaatac gcaaaaatat cgaacaaaaa gtcattacac
     1021 ttgttcttgc ctattatgtc gatcattgtt gggatgaaat agaaatagaa ggtggatgtt
     1081 tacaaactaa taattttaaa caattagcaa aacatggctt aatcattaaa gctagaagag
     1141 aagctgaagt cgttgacttt ttacagattc aacgggagaa tgctccaatc ttttttgaac
     1201 atgaatctct tggctggaaa aaagaaaatg aagcaatgat ttttcagtta caagattctt
     1261 ttggacgtga aattccttct gtattaacta aaaactcatg ctaccaatta acaactaatg
     1321 gtacagaagc ggaatttgac gcattggttc ataagtatgt tttaggaacg aacttagaac
     1381 ttgcttggtc gattggtatt gcttctattg tgttaggcta tttaaacgtt acgaaggcta
     1441 atactttttt gagtttacta attaacctac agggcaagag cacaacgggt aaaaccacta
     1501 cgttacactt tattgcctct ttatttggaa atccaactac ggttcttaga aatatgaatg
     1561 ctaccaataa agccattata aagctagcat caaacaatca gggaggtgtt ccactaatac
     1621 tagatgagtt aggagcagca actcaaaata actttagctc gttgatctat caattagctt
     1681 caggagaaga aaggctaaga ttagattcta attgtcagct tatgccacaa gagacttttt
     1741 ctgttttgac ccttacctct tctgaagagc gtttggaaaa ttatttcaat gagaaattaa
     1801 ctggtttgaa agttcgatat atcgaatttt ctgatatcaa gtttacaaag gatgcgaatc
     1861 acgcagagaa agttaaaagt ctttcttcta atacttttgg ttggggtata aagttatttc
     1921 ttttgaaact ctttgaacaa gggattacaa aaattgaagc aacttttgtc gaagctcaaa
     1981 aaaaactatg ggctgtttta cctgaacatt ctttgaagca acgcattggt aataatctag
     2041 ctttgattta tagtggagcc ctcctaattc aagatatact gggctatcct ttacgattag
     2101 agcatataca gacggtgctt attaattcgt acgaacagtt aattgtagat actagtttag
     2161 gaagtgagca aacggtattt gaccgagtga aaacattgtt ggtgatgaat agccataaat
     2221 ttttaacaaa cagtgacaac aaacatacgc ccagtacgat ttggggaaaa gtatcaattc
     2281 aaaaaaatgg ggatattaag gtaaatattt ttcctatccc gtttgaggac ttgttaaggc
     2341 gagaatttca agtaaaggat ctaacctatc tttttaggca attgcttgat tcgggtaata
     2401 tgtgtgcaga aaaagggaga agaacgaaac gtattcacat gaatcgtcaa gttcttccaa
     2461 catacgagat tctattacct tcttcactca aatcgtattt tcgattgtaa tttagaataa
     2521 accaaagtat gaaagtgagg caccatgtta tggaaatatt tcttattgat ttagcaaaac
     2581 aactgattac ttatctgtat gatagcgagc aaacccaaac ggaagtaaat gaagcttgat
     2641 ctatgcttca tttacttcat tcaatcaaac atgaaagaaa ggaaaattct aatgactaaa
     2701 aataaaatga cactaaaagc agaggttctt ctgtatattc aagaacattt ttcaaatcaa
     2761 gcctttttta caaaaccaat ttacttggat ttcgaaataa gaggggtatc tgctggatct
     2821 atcgggggta ctcttcaagc gttgaagaac gaaggatatt tagaaaatca ctttgtgcaa
     2881 cgttcgttca atagaagagt cgtgaaaaaa tggtatttag ttcattcata gagaggggaa
     2941 tcaagcatgc gtgtggcttg ttatgatcga acgtctgttt atgaagaagc gaaaattagt
     3001 aatgacgctc aatatgatgc agtgaaagag tatgttttag agaaatatga acaaattccc
     3061 caacgatttt cagacaaagg agaatcaggg gaaaaagcgg agcgtccagc ttttcgacag
     3121 cttagagctt gggtttgtgc gtcagaagaa gagggggtga tctatgtttg gcgttatgat
     3181 cgattggcac gtcaagccaa aatagcctta gaatttattg agttgtgcca acaacatcag
     3241 gttcaagtgt attcgatttc tgaacctctt cctgcttctt tctcacacgc gtcaacgaat
     3301 aaactatttg tgcaattatt gttcactttt gcagaacaac aacgaaacgt cattattgag
     3361 aatgttcgtg ccggactttc ctataaaaag caacaacggc aatatttgtc ttctgcggta
     3421 ccatatgggt atcgtttagt gaatgggaaa atccaacaag aagtacacga agcaaggatt
     3481 gttcgctata tttttcaact ttatttgacc aaaaaatatg gctacaaaaa gctttgtcaa
     3541 cgattgacgc aacaaaagct tttcttccgc gaacgtcctt ttcagcccta tcatatttat
     3601 agtatcctaa aaaatccatt gtactatggc gaaatcaaag gaggatcttt ggggaagtat
     3661 ctaggaacct ttgaaccgat tctctcgaaa gcaacctttt tacaagccca agagattcgc
     3721 caatcaaggt gtacagctaa aaaagatacg tatctgtact tattacgtca aaaaattcgg
     3781 tgtcccttct gtggtcgtca tctgagttcg aagtatcaat ggaatacaaa gaagacgaag
     3841 acgcttcatt attatcattg taccgaccga agctgccaag gaatttttct tcgggcagaa
     3901 agggttgagt cagaagtatt ggaaaagata caggcttttt ttcaatacga aggcgtctat
     3961 caagccttga ttgcccaatt aacgcaacaa ttagccgaaa agcgaaaaga tgaaaaacgc
     4021 aaagtaatcc aacaaaagaa acaagaagca caatggatta aggcatttga agagggcaat
     4081 tgttcactgg aagaactcaa gcaacaactg caagctcttc aaaagccgat gtctacttct
     4141 tcgtttcttt cactccaaca gtgtgaagca aaagtgaaag aatggcttca actaaaagac
     4201 ctctctagtc gacaattatt atgtgactat gtttgtcgga ttgaggtaac aaaggataaa
     4261 acagtttgtg ggatttactt ccgacaactg acacaaaata ttttagaaag gaacgaatga
     4321 tctatggaaa tgatacaaac caaacgagtc gcattgtata ttcgcgtgag tacacaagaa
     4381 caagctttag aaggacatag tattcaagca caagaacaaa atggacgaca gtttgccgaa
     4441 cggatgggct atgaagtggt agaagtttat gccgatgaag gaatcagtgg gaaatccaca
     4501 aaaaatcggt tcgcctatca acgaatgatg aacgatgctc ggaaccatcg ttttgatcta
     4561 ctagtgattt ggaaattgac ccgtctcgga cgcaatatgt tagatgtctt acaaacagtc
     4621 gaggaattat tgcgatatga cgtaggatta cattcgatct cggaacaatt tgacgtgacc
     4681 acttcgacag gtaaattgat gttacaactc cttgggagtt tcggtgaatt tgaacgcaac
     4741 caaattgcgg agaatgttca aatgtcaatg caaagcttag tgaaagataa aaagcgttat
     4801 gcaggaggcc gacgtttagg ctatgtcagt ggtattgaca cgaatggtca aaaacaattg
     4861 gtcattgaac cagaagaggc gaaaattgtt cgtctgatct atgcaaaata tttaggcgga
     4921 gcagggtatc gagcgattgc caattttttg aatcgtcaag gatatcaaac agtcaaaaag
     4981 aatgcttttt ctaccacggc agtcaaagat atcttacgca ataaaattta tgcagggtat
     5041 ttagaatatg cgcgctacat caattgggaa accaaacgac ggaaggggaa gaatccgaga
     5101 ccgattttag tgaaaggaac ccatgaaccg attatttcgg aagaagatta tcaaaaagta
     5161 caagaacgcc ttgctttaga aggaaaacaa cccaaatgga accatacagg tgaaaatgta
     5221 ttgacaggct tgttacgttg tccagaatgt ggcgcaccga tggcagcgag taatgtcaca
     5281 aataccctaa aggatggcac aaagaaacgg attcgatatt attcttgttc agtctttcgg
     5341 aataaaggag caagtgtgtg tcatgccaat tcgattcgtg ccgaacaagc ggaaacattt
     5401 gtcgccgacc gattaaagga gatcgttcag ctaccagaag ttcttccccg attggtggca
     5461 gcgttaaatg aagaaatagt gcgacaaagc caaccattgg aacaagaact cgtggtgtta
     5521 ctggaacgaa aggaagaatt gaagactaaa attgaaaaat gggaagctgc tttggaagat
     5581 agtcctgaat tgtttccgat gttaaaagac cgcttagacg aattgactga aaaacgacga
     5641 cagttgcaca ttcgagaaaa tgaaatctta gggatctttc aacaaggaga gccgattcaa
     5701 gtcaaagatg tccaacggat attgactaat ttggatcgtt ttcttgccca gagtgagaag
     5761 aagcaaatca aagccttgta tcgtaccttt atagagaaaa taacctttga tcctaaccat
     5821 aaggagatgt tggaaattac gatgaaattc acgcatgccg tggtacaaca attgaatgag
     5881 caatatcaaa tagccgtatc caaaacaaag gatacggcta tttttgtgtt aaaaacaccc
     5941 tttacgttaa cgatctagtc taggttatat caaacaacgt tagaggggcg ctaacgcttc
     6001 tctagcgatt tgaaatcgtt agagaagtag ttagggaggt ccttatcata aatagggata
     6061 tttattaatg aatatttgaa atataattgt atataagaat taaaaagagt ggggatagat
     6121 atgaaaaaga tgggtaagtg tattttatgt gaaaaataca ctgaacttac taaagaacat
     6181 gtaccaccta aaaaatcagg gaatactgga ggtaaaaaga ccagaactgg taatttagat
     6241 gatttcctta agagcgattt tacaaaaggt gattttccga aggggattaa aaggaaacct
     6301 caaggaaatg tttattata
//
LOCUS       DACDMM010000141         6303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDMM010000141 DACDMM010000000
VERSION     DACDMM010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.142"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            334..714
                     /locus_tag="IWD16_002259"
     CDS             334..714
                     /locus_tag="IWD16_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9089258.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="IWD16_002260"
     CDS             696..1610
                     /locus_tag="IWD16_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9089259.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="IWD16_002261"
     CDS             1855..2544
                     /locus_tag="IWD16_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089260.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="IWD16_002262"
     CDS             complement(2665..2751)
                     /locus_tag="IWD16_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9089261.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="IWD16_002263"
     CDS             3427..4377
                     /locus_tag="IWD16_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9089262.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="IWD16_002264"
     CDS             4398..4931
                     /locus_tag="IWD16_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9089263.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            5567..6100
                     /locus_tag="IWD16_002265"
     CDS             5567..6100
                     /locus_tag="IWD16_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089264.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2324 a         1102 c         1173 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtatacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DACDMM010000142         6287 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDMM010000142 DACDMM010000000
VERSION     DACDMM010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.143"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..517)
                     /locus_tag="IWD16_002266"
     CDS             complement(<1..517)
                     /locus_tag="IWD16_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9089265.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGV"
     gene            complement(650..1333)
                     /gene="rpiA"
                     /locus_tag="IWD16_002267"
     CDS             complement(650..1333)
                     /gene="rpiA"
                     /locus_tag="IWD16_002267"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP9089266.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            1652..2065
                     /gene="rpsL"
                     /locus_tag="IWD16_002268"
     CDS             1652..2065
                     /gene="rpsL"
                     /locus_tag="IWD16_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP9089267.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            2149..2619
                     /gene="rpsG"
                     /locus_tag="IWD16_002269"
     CDS             2149..2619
                     /gene="rpsG"
                     /locus_tag="IWD16_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP9089268.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            2713..4800
                     /gene="fusA"
                     /locus_tag="IWD16_002270"
     CDS             2713..4800
                     /gene="fusA"
                     /locus_tag="IWD16_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9089269.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            4972..6159
                     /gene="tuf"
                     /locus_tag="IWD16_002271"
     CDS             4972..6159
                     /gene="tuf"
                     /locus_tag="IWD16_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9089270.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT         1919 a         1247 c         1295 g         1826 t
ORIGIN      
        1 aaacaccgca ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc
       61 ggattttttt gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac
      121 gagcttcatc taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta
      181 ggttttccat taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa
      241 gactaatcaa gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg
      301 tagatttgcc taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag
      361 cgatcactga ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat
      421 ggaaaatttc ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt
      481 catttatggt tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat
      541 tttctaactg gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt
      601 cgcaaatttt ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac
      661 ggattgcgtc aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt
      721 ttaggaaaag cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt
      781 ctggctgttc gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta
      841 agacttctcc gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat
      901 gaagtagctg ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt
      961 tatcgactaa tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta
     1021 ttttctcgaa aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt
     1081 catcagcacc gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta
     1141 ttcctaattt ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta
     1201 aaccttcttc acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg
     1261 tcccgagacc tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt
     1321 gttttaaatt catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta
     1381 ttttagcgtg atttacggta aaaagatacc attctttttt attccaataa agctttttca
     1441 aaaaaaattg catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct
     1501 ttatctacag gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg
     1561 atgcagacat tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta
     1621 gacgcaaatc gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa
     1681 acctcgtaaa tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag
     1741 ctttaagaaa actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt
     1801 gggtacaatg acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt
     1861 gtcaaactta attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca
     1921 cagtgttgta ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat
     1981 cgttcgcggt gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata
     2041 cggaactaaa aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact
     2101 atatagaagt aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa
     2161 ggtcctgttg caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact
     2221 cgcttgatca accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat
     2281 aatgcctttg acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa
     2341 gcaatgaaaa acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac
     2401 taccaagtac cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta
     2461 gtaaactacg ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc
     2521 atggatgctg caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg
     2581 gctgatgcca accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc
     2641 ttttataagt cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga
     2701 gaaacaagga gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg
     2761 gcccacatcg atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa
     2821 atccataaaa ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa
     2881 caagaacgtg gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga
     2941 atcaacatca tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg
     3001 cgtgtacttg atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact
     3061 gaaacagttt ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa
     3121 atggacaaga tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa
     3181 gcaaacgctc acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc
     3241 gaccttgtaa caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa
     3301 acagaaattc ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa
     3361 gctgtagctg aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact
     3421 gaagctgagt taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta
     3481 ctatgtggtt cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat
     3541 tacttgccat caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa
     3601 gaagtagaac gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg
     3661 actgacccat tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca
     3721 ggttcatacg tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag
     3781 atgcatgcca actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct
     3841 gttggtttga aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt
     3901 cttgaatcca tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa
     3961 gctgaccaag ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc
     4021 cgtgctgaaa caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac
     4081 ttagatatca tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg
     4141 cctcaagtat cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta
     4201 cgtcagtctg gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa
     4261 gaaggtgctg gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac
     4321 attccagctg ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat
     4381 ccattagtag atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat
     4441 gaaactgcct tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat
     4501 ccagtaatcc ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt
     4561 gatgttatgg gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct
     4621 ggcggccaac aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca
     4681 actcttcgtt ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa
     4741 gatgtaccaa aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa
     4801 tttcttaaaa ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata
     4861 gtttcgtgta aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat
     4921 acctatcaga caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa
     4981 gaaaaatttg accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat
     5041 ggtaaaacta cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa
     5101 gctatggctt acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc
     5161 tcaacagctc acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca
     5221 ggacacgcgg attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc
     5281 ttggtagttt ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct
     5341 cgtcaagttg gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac
     5401 gaagaattac tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc
     5461 cctggtgacg atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct
     5521 tcatacgaag aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca
     5581 gaacgtgaca acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga
     5641 cgtggtactg ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt
     5701 gaagttgttg gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc
     5761 cgtaaattgt tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt
     5821 gcacgtgaag acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat
     5881 acaaaattct ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca
     5941 ttcttcacta actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt
     6001 gaattaccag aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa
     6061 ttaatccacc caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt
     6121 acagttggtg ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa
     6181 agcaacattt attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc
     6241 ttttttattt attgcaggat caggaaaaaa gacaggagta ttataaa
//
LOCUS       DACDMM010000143         6127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDMM010000143 DACDMM010000000
VERSION     DACDMM010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.144"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..528)
                     /locus_tag="IWD16_002272"
     CDS             complement(<1..528)
                     /locus_tag="IWD16_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089271.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNY"
     gene            complement(628..1137)
                     /locus_tag="IWD16_002273"
     CDS             complement(628..1137)
                     /locus_tag="IWD16_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP9089272.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1338..2207)
                     /locus_tag="IWD16_002274"
     CDS             complement(1338..2207)
                     /locus_tag="IWD16_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9089273.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2227..3348)
                     /locus_tag="IWD16_002275"
     CDS             complement(2227..3348)
                     /locus_tag="IWD16_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9089274.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(3350..3745)
                     /locus_tag="IWD16_002276"
     CDS             complement(3350..3745)
                     /locus_tag="IWD16_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9089275.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3764..4561)
                     /locus_tag="IWD16_002277"
     CDS             complement(3764..4561)
                     /locus_tag="IWD16_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9089276.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4563..5351)
                     /locus_tag="IWD16_002278"
     CDS             complement(4563..5351)
                     /locus_tag="IWD16_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9089277.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(5365..5841)
                     /locus_tag="IWD16_002279"
     CDS             complement(5365..5841)
                     /locus_tag="IWD16_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9089278.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(5913..>6127)
                     /locus_tag="IWD16_002280"
     CDS             complement(5913..>6127)
                     /locus_tag="IWD16_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9089279.1"
                     /translation="FGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSF
                     SVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         1894 a         1329 c          870 g         2034 t
ORIGIN      
        1 ataatttttt aaaatctcat catattttgc aaatatttca ttgccatatt ccgtccttaa
       61 aattatattt tcagtgccag acaagtgatt tatattgagt tgataaaaaa gatctccatc
      121 taaaaaaata tcattagaag aagatttccc cacagtaatg accattccat cagctctatg
      181 tgttacatac aaataattgt atgacctcat ttcacctaat gaatcaatta tttcttgccc
      241 tttagctcta atatattgat ctctattacc tatctcactc ttatttaaca aaagtttttt
      301 catgtcaaat ttaatcggct gttttatttt tgtaaattcg cttctactac taatccaatc
      361 cgagattaat ttttcaacat gtcccagagt aaactctgat ttattcgcta aatacttttt
      421 ttgttgtatt aaagacatct ttccccgaag ctcaaaattg agcctacatc gattctctat
      481 ctcttcaaaa agcatcttat cttcatcatt cttatattca tcattcataa aaccaatcca
      541 gctcctcgat taagataata ttttaatctc atacaaaaac tagctctcat caaaacaacc
      601 attgttttga tttctcatga aatttcttta tacttttctc cgattataaa ttcctaaccc
      661 cttgagaaaa ttacgcatat atttatctcc gcaaggctta aaatttcgtt ggccttcttt
      721 tctcaaaaat gccccgattt cgccttttga tggatagatt tctgcctcag caaaaacgtc
      781 taaaatatca tcagtggtca tagacaaggc gatttttaat ttttttagca acacattgtt
      841 gatatatttg atttccttgc ttttttgcgg cgtgtctact atcggcttat ctttttgcgg
      901 accacgcgcc aagagaatca ggccatttag gaatgcgtca aaaacttggt tgttgatggt
      961 tttttcatac acattatttt ctgcattatc tttgttctta tcctgtactt tggtcagtac
     1021 cgcctgagct tcttctttag tcagtggtac accgcctagc tcaaaagctt taatcaaatc
     1081 cgtatctttc aaatcaatcg cataacgcaa tcgcaataac ctatcattgt tattcatttg
     1141 ttcctccgaa gtatttagtc tttactttct tacgtacttt ttgtcttgct aagattttta
     1201 ggatcaatga taaaaaccgc atcacttatg ataaggcgct gggtatcatg agtgaatgat
     1261 attgctgaat acaaatagtc cgaccacatt aagttgatcg gacttcataa gcattttatt
     1321 gactaatact tttttgctta agaattgatt ctaacatctt ttgctaaagt taagcatcct
     1381 atagttcctg cattatctgc taacttaggt gtgacaatgt attcttcgat atttggcgta
     1441 tcgacatagc cgttaattag ttctaaaaaa cgtctttgta ctttcttttt cagatgttct
     1501 tgttgcatga caccaccacc aaaaattata atatctggtg aaagcatcag ggtcgtatta
     1561 caggcacatt gtgcaatata atccgcctcg atttcccaga acggatcttc tatagaaaga
     1621 tcttgtccct tggttcccgt acgcatctcg atagcgggtc cagatgccaa cccttcgaga
     1681 caatcatgat gaaaaggaca agtaccagag aaagtatctc ctgaatgtct cttaactaac
     1741 gtatgcccca tttctggatg gctaaaccct tcaacaaaaa tcccatcttg tatagctcct
     1801 ccaccaattc cagttccaac agtgtaataa acaacactgg ataaaccttt tccatgacct
     1861 gaaacatact ccccgtaaca agcagcattc acatcggttg tccaggcaat tggaatttcg
     1921 aaatgcttat tcaacgttcc aacaaaatca aaatttttcc aggcagtttt tggagtagag
     1981 gtgatatgtc cataggtctt tgattcacga tggatatcaa tcggtccaaa tgaccctact
     2041 ccaatgcttt ctaattgttc tttatagtga ttaaagaaat caattaccaa actcattgtt
     2101 tcattcggtg tagttgtagg aaaactcact ctttctaaaa cctttaaatc atcattgcca
     2161 atggcacaaa caaatttagt cccacctgct tctattgagc catacatttc ttactccacc
     2221 gtccttttat ctaggaattc gttcttccct gtctaatagt ttttcgaaaa actcatgtgg
     2281 ttccttttgg taccaatagg caacagaaga aatatcgtct tgacgctcat aaagcttgat
     2341 gtcatcattt cctagggttt ggaaagtaac acgtaaattt tctttgaaac taatggggtc
     2401 gggtaaatgc cagcggtaca acccatgcat cggtaaagca tcatcgccaa aaccatgaac
     2461 tggatttgga ttaggttttc cagcactaaa ataatcccgg gtgtgatctc ttttagattg
     2521 aaaaggatag cccataaata gcgtattaaa attttgagca tatgcccttt ctccaaagtt
     2581 ctttttatgg aaagcccaag cgccgccaaa ataatcttct gctcctgtag atgttattgt
     2641 agggaaatct tcatctccat ctatataaaa cttaaattct ccttcccccc accagtatct
     2701 ttccaatgcg cacaaagcaa agtaggttcc tacataatac ccagtgcctt tgattccatc
     2761 aataagggtg taatcttttc cttttgtcgt gatgggttca cggttccatt tagcatgaaa
     2821 atacatcgca tcatccggta cttcagtcaa tgcataatta aatgtataaa aaatgtgctt
     2881 aataaactca ggatgttcat tcgttaaagt aatcttagct ttttttcgaa atggcatctc
     2941 aaaatagcaa ttcatccctg atgttggatt cactacgatt ggtaaggaat tcacttcgca
     3001 tcttgtccca aacccattac aaaaaaaatc tcctaatgga ctttctacag aaggagtgga
     3061 ttcatcatcc caataaatac gcaaaattaa atcacgtaag acgaaactcc cagcgattgt
     3121 tttgtctgtc actgtcatcc aaaaatgacg taaaattcca gggccttcca tttctgctaa
     3181 agtaattgtc tctccagcat ttatcggaac agaaggtgac ccttttctcc caattcctag
     3241 atgactcgct gtcattgccg ctttcccttt ttcaccattt ttattttctg gtgaaatagt
     3301 tctactttcc tctcccttgt ggagccatat attatttaaa caattcattt tattcgtcct
     3361 ctatcctttc ttcacttttt tgattattca tatagatgat ttgttgttgt gcctccgcaa
     3421 taattgctcc aatatctaaa ttatcatttc ctaactgcaa agcgattgac attgccaagg
     3481 gaagattcat tcctgcaatt acgtgcactc tgtcagaaat ataggtaaaa aacttttgat
     3541 ttacacttcc tccagccatg tcagttaaaa caattatttc gtctgtagta tccactaact
     3601 ctttaatttg ttcatctata tcatcaattt cttcgacata tgcacataag ctactaattt
     3661 gattatgcat acctgttaaa aactctagtg tctctttaaa accctcagct agtattgaat
     3721 ggctagcaat gatcaatctt ctcataattc aaatcctttt cctttacgct aatattccaa
     3781 atgcagccat aaccatcgca aaaacaataa cgagtagaat caaaatggtc atactgattt
     3841 ttttcttact gagtagataa tatgctgcag cagtaataat tactggaatt aatgttggta
     3901 aaattttgtc caacattggt tggattttca tactaacttc accaaaagtg aatttaagtg
     3961 gcgttgaaac actgataact gaagcaatta atgatccaac tactgttagc cccaatactg
     4021 aagcagcttc agttaaataa ttaatctttt caccgtattc agtaacaatc ttcattccct
     4081 gcttataacc aaattcaaag cctctcattc ggaaaaagaa gattaccatg attaaaatga
     4141 accataaaaa gattcctaca ggattatttt ctaaagccat ataagccgca attgatccca
     4201 tgatggttgg gatcatcgtc atcaaaagag agtccccaat accagcaatt ggacccatcg
     4261 taccaacttt aaagtcatta atcgcatctt ttgcttctaa tccttgcttt tcctccattg
     4321 caacacaagc acctaaaata atggctgcca gccaaggctg tgtattgtaa tactgaaagt
     4381 gattatttaa agcttcgatg tattcatcat catctttgta taattttctt aaaataggtg
     4441 acaatgcgta agtcacagat gcaccttgct gtgtttgata attaaaggta caaactgaca
     4501 tcaaccaacg atagttcgct ttcctaagat cctttttcgt tacttgatat ttttcgctca
     4561 ttttattcat cctccccaat aaacccaaca tcagaagctc ccgcagaagc agtgatttgt
     4621 gcatcttttt cagctctatg atacacatta aatgctaaag cgaatcctat gatcgccgct
     4681 ccaataatag gtaccttcaa aaaagcggct aagaaaaatc caacaagtaa ataactaatg
     4741 tatcgacctg taggcataaa gcgtaacaac atcgcaatac caacagcagg caacattttt
     4801 ccggctaatg ttagcccatc cataaaccaa ttaggtaaaa catccaaaac gtaattaaca
     4861 gcagactgtc ctaaagtaac ggcaataaat actggaattg ctgcagataa accaaagaaa
     4921 attggcccca accacaaaac actattcatt ttcttaaatt ctcgtttatt cgcatattcc
     4981 tgtgatttgc gaacaacaaa accatttaag attttgacaa ttacatctag ttgcacccct
     5041 aacataccta caggtagtcc tattgctaat ccggcttcta ctccaagctg gctaccaaat
     5101 gccgttgcaa taattgcgca cgttccatag tccggtacag atgagccacc aaaaccggca
     5161 actcctaatg acatcaattg aactgtaccg ccaactatca aacctgtttt tatatcaccc
     5221 atcaaaattc ctgtgaataa tccccaaaaa actgcgtaat ttacaaagat catcggaatc
     5281 ccataataat caacatgttt aataaatgct aatatagtca gcataataat ttgaaaaata
     5341 gtaaaatgca ttatccttac tcctttatcc taattttgat tgaatttcct gtgccttatc
     5401 cgccggaaca aattgtgtaa caatttctac accagaatcc aacaattttt tatacgtgct
     5461 gatattttca tctgatgcat aggctctaga agatagctgt aatgcattat ctttaacaac
     5521 agtaccgcca acaattagtc ggtcaattct aataccgtta tctactaaat gaaggattgt
     5581 ttccggctct ttcacgatga caaatacttt ttcctgatcg tatttcccct ttttaaaatt
     5641 cgcaatcgct gtttcttcag tgataattga tacagccatt ccagcaggtc tagctaaacg
     5701 catacttgat ttttggatgt cgttatttgc gacagaatca tcgatcacca tcactctgtt
     5761 tgctttagac attggtgccc attgcgtgac aataatccca tgcaataaac ggttatcaac
     5821 tctcgacata acaatactca taaatacttc ctcctttatc tttcattttc ttacaagcaa
     5881 ttaccatgcc aaaacaaaaa gaacttgata aatcaagctc tttttacata atcataaacg
     5941 tatttaacct catgaatcgg gatgtcgaca ctataataat gttcaacgac actgaaagat
     6001 tgcttaaaat gtgtctgaaa tttttgaaat tcctcactcg tttcattgaa ataaatttca
     6061 tcttccaaag tattttgttt tactaatcgt tcaattaagc aactaatgtg aacatataat
     6121 ccgaaac
//
LOCUS       DACDMM010000144         5809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDMM010000144 DACDMM010000000
VERSION     DACDMM010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.145"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            450..1604
                     /locus_tag="IWD16_002281"
     CDS             450..1604
                     /locus_tag="IWD16_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP9089280.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="IWD16_002282"
     CDS             1598..2020
                     /locus_tag="IWD16_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089281.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="IWD16_002283"
     CDS             3143..4075
                     /locus_tag="IWD16_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9089282.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="IWD16_002284"
     CDS             4092..4607
                     /locus_tag="IWD16_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9089283.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="IWD16_002285"
     CDS             4903..5184
                     /locus_tag="IWD16_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089284.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="IWD16_002286"
     CDS             5186..5602
                     /locus_tag="IWD16_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089285.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DACDMM010000145         5780 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDMM010000145 DACDMM010000000
VERSION     DACDMM010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.146"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(89..1219)
                     /locus_tag="IWD16_002287"
     CDS             complement(89..1219)
                     /locus_tag="IWD16_002287"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP9089286.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1867..3069)
                     /locus_tag="IWD16_002288"
     CDS             complement(1867..3069)
                     /locus_tag="IWD16_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9089287.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3129..3332)
                     /locus_tag="IWD16_002289"
     CDS             complement(3129..3332)
                     /locus_tag="IWD16_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9089288.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3474..4706)
                     /locus_tag="IWD16_002290"
                     /pseudo
     CDS             complement(3474..4706)
                     /locus_tag="IWD16_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4869..5681
                     /locus_tag="IWD16_002291"
     CDS             4869..5681
                     /locus_tag="IWD16_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9089289.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1709 a         1189 c          921 g         1961 t
ORIGIN      
        1 tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg actttttggg
       61 acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg acttgctgca
      121 ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct tctgttgaag
      181 caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt tgtgcttctt
      241 ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa gtaactaatc
      301 ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa tctcctgctc
      361 ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac tcttctgcaa
      421 tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg cagatatgtg
      481 ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc tctgtacaaa
      541 tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt ttagtttgat
      601 cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct ttttcataaa
      661 aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca gatagatcag
      721 gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt cgtaaaatca
      781 tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc actacttctt
      841 tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat ttatctacta
      901 cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat ccagtttctg
      961 gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa aatccacgcc
     1021 gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc actaattttt
     1081 ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct tcttgaactt
     1141 tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga tctactgtaa
     1201 atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa aaaaatcctt
     1261 ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt tttcagaaaa
     1321 aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa agactgttcg
     1381 tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc aacttctagc
     1441 tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct tctcatcgga
     1501 tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg gaataagaac
     1561 aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata aagcttttct
     1621 aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa tgatcatacc
     1681 cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa actaagtcct
     1741 ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca tcttgaacag
     1801 gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg tagaaccgtg
     1861 gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct cttgaacatt
     1921 atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt aagttcctaa
     1981 agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt gaatattctg
     2041 ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg gtttgacacc
     2101 cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg taaatgttcc
     2161 gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat accactgtct
     2221 gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt tagttttggg
     2281 agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa tcgttatcgt
     2341 gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa tccgaatgcc
     2401 agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct tatcatcctt
     2461 caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt tcttattctc
     2521 agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct ctttaacagc
     2581 ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt ttgataacgt
     2641 ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt caaataacca
     2701 atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa actgcatacg
     2761 cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc tctcgtacca
     2821 ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag aacacagttg
     2881 attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag caattttggc
     2941 atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat tttcatctat
     3001 acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa tatttttaat
     3061 ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata gtaccgcgct
     3121 tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg ggatggcacc
     3181 acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag atatgccttg
     3241 ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat caaaattgca
     3301 cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc tttactcctt
     3361 ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt atactcgtct
     3421 tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct tttttagtag
     3481 cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac ctcctcctga
     3541 taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg attcatttct
     3601 ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac ctgtgcaata
     3661 aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc aaaactttga
     3721 ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc tgcccatttg
     3781 gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat tgctccatta
     3841 agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt tgccaagctt
     3901 tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc ctcttctaaa
     3961 ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa acgacactga
     4021 ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat gatatccgta
     4081 ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat taccttatgc
     4141 ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc aatccgtgta
     4201 aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt ttttcgtgtt
     4261 tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac agttccttta
     4321 accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta ttggccaata
     4381 tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc aataatctct
     4441 tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc aatcaaaaca
     4501 cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa tttttcttca
     4561 cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat ccccactttt
     4621 tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat ggcttcaccg
     4681 agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta cttgtcaaag
     4741 aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat tttaagtcaa
     4801 tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta tcctgaggag
     4861 gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag attcgccaag
     4921 aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg cgaagtacag
     4981 tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac caaatcgcag
     5041 agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat caatacattt
     5101 tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt ctcatagtag
     5161 acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg attgaatttt
     5221 ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa tgtatcgatg
     5281 aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag ctctatttgc
     5341 aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc aactttagtg
     5401 tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt gggaaacatt
     5461 tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa ttgaccagcg
     5521 atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag tacacagagg
     5581 acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg gaagtcgaga
     5641 aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa attgataagc
     5701 acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac agtttcctgt
     5761 tctttgttag ccccctgtta
//
LOCUS       DACDMM010000146         5758 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDMM010000146 DACDMM010000000
VERSION     DACDMM010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.147"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..3116)
                     /locus_tag="IWD16_002292"
     CDS             complement(<1..3116)
                     /locus_tag="IWD16_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP9089290.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLD"
     gene            complement(3130..4347)
                     /locus_tag="IWD16_002293"
     CDS             complement(3130..4347)
                     /locus_tag="IWD16_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9089291.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4337..>5758)
                     /locus_tag="IWD16_002294"
     CDS             complement(4337..>5758)
                     /locus_tag="IWD16_002294"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9089292.1"
                     /translation="TVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1557 a         1224 c          975 g         2002 t
ORIGIN      
        1 tcatctaaca ttttacgcca tgctcgcttc atttcttgtg ggtatttaaa tggtttcgct
       61 tctggatgtt tggctttata tccttcgtaa tccttactgt tgataattgc tttcatatta
      121 ggaattggat cttcacctgg catatattgc agattagaag catgtaattc tttctcagaa
      181 acaaaccact cattggaaaa ggcatgaatc aaattatcac ttgcttgtgt aaaatagcga
      241 cgtttaatga caaagatatc ttcattacta tctaaagtcc ccacatcaaa tgcatttaaa
      301 atttgtcggt aaacttcttt gactccttct gttttattca atttagccaa agctttttca
      361 atttcttctc gtgcatctga actattcgca gcataagttc ctagtaattg atcaatataa
      421 gtagaatcaa tatcatacaa cttagtggta ttttcactat aaaattcgat cgttgaaaaa
      481 tcttctccct ctttttctgg ttcttcctcc atcaatgaac ccttcacggt ctcgtacacg
      541 ccaatttgtt cttccaaaag ttgaatttga ttgttcaaag ttgaagattg caccatgtca
      601 tcattgtaat catcataagt cactaacgat tcatacgcac gattcaattc ttgaaatgct
      661 ttgacgtact cgatacggat ttcaagtggc gtatgttcat caatggattc catggatgaa
      721 acctctttta acttaccata tgcttgcttg aatctttttt tggattcttg gtaacttgga
      781 taaatcaaac ccgattcttc tttagcttct gaatacagtt ttgtcgcatc ctcgacattc
      841 ttctccattg tcgctggctt tcggaaagta acaattagtc cttttgtttt ttcaggatac
      901 gtacggttcg tacgtgaaaa ggcttggatc aatcctgcat actctagatt acgatccaca
      961 aatagggttt gaatcgtagg ggcgtcaaaa cctgtcaata gacgatccac tacgattact
     1021 aaatcgattt gtttcccaaa ttgtttgaat tccgctcttt ttcgtgccaa acgattattg
     1081 atgtctccgt tataacgctc aatgtctgct agtgaccagg cagtatcata atactgatta
     1141 tattcctcaa taattttttg catttcttct tgttgggcac ttgaatctcg gctattttct
     1201 tctaatgaat aggtaatagc gattcgtggg aaatcaggat cctccatcgt tcgtccttca
     1261 cggataggct gattgggaaa ctcttttatt aaccaatcag attctttcgt catctctttg
     1321 atggcccgat aatacttttt ggccatatca atggaactcg tggttaagat cgcagacttc
     1381 gttggacggc catttcgaaa atcaaatttt gtataggcat tgtctggacg gaacatttta
     1441 tgaatgactt tttggatatg ttcctctgct tcaaaaacag tagggtccaa ataactttct
     1501 tttttgacag actccatttg atcgatcata cgattgatct gttccggtga atactctgca
     1561 tatgtttcta cttcttttaa tttccgatag attttattct ctaatgaagt cggctccacg
     1621 gtgttttcat gctctacttg aaatcctaaa accgagccat cttctaatgc attcttgatg
     1681 gtataggtgt gcaagacctc tccatactga tcatgggtcg tacgtgccaa ttgccctttg
     1741 gcttgtttcc gattctcagg gaaaatcggt gtacctgtaa agccaaacca agtggatttc
     1801 ggaaacattt ttttgatttc ttccatattt tcagcactta acgctcgatg acactcatca
     1861 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
     1921 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
     1981 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
     2041 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
     2101 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
     2161 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
     2221 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
     2281 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
     2341 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
     2401 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
     2461 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
     2521 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
     2581 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
     2641 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
     2701 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
     2761 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
     2821 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     2881 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     2941 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     3001 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     3061 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     3121 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     3181 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     3241 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     3301 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     3361 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     3421 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     3481 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     3541 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     3601 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     3661 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     3721 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     3781 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     3841 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     3901 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     3961 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     4021 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     4081 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     4141 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     4201 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     4261 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     4321 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     4381 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     4441 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     4501 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     4561 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     4621 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     4681 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     4741 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     4801 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     4861 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     4921 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     4981 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     5041 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     5101 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     5161 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     5221 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     5281 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     5341 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     5401 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     5461 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     5521 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     5581 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     5641 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     5701 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgt
//
LOCUS       DACDMM010000147         5756 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDMM010000147 DACDMM010000000
VERSION     DACDMM010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.148"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            582..1550
                     /locus_tag="IWD16_002295"
     CDS             582..1550
                     /locus_tag="IWD16_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9089293.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1561..2376
                     /locus_tag="IWD16_002296"
     CDS             1561..2376
                     /locus_tag="IWD16_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP9089294.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2444..3154
                     /locus_tag="IWD16_002297"
     CDS             2444..3154
                     /locus_tag="IWD16_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9089295.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3190..4431
                     /locus_tag="IWD16_002298"
     CDS             3190..4431
                     /locus_tag="IWD16_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9089296.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKNESSPQSCVKVQPHQKH
                     NVLRCEPNCHAFLSEERGQEQ"
     gene            complement(4489..5676)
                     /locus_tag="IWD16_002299"
     CDS             complement(4489..5676)
                     /locus_tag="IWD16_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP9089297.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT         1867 a          958 c         1159 g         1772 t
ORIGIN      
        1 agtctttatt ttttctgaat ataactactg tatcaaaaac aatcgattat gagacaaaac
       61 aaaatgacct cttttattta agaaaggagg tcattttttg tatcataaat tgtattaaaa
      121 tcaatgtatc actttttaat cagttttaag accataaatt gcgtactaaa agaaattctg
      181 caattcaaaa taaagcattt taagccattt tcaaatttaa gtgcacccat ctacccgtca
      241 aatcacttta ttttccactc cagtagtttc ctaattcaac tgtgtgcaaa aacgatttct
      301 tctaaacaaa tcaagacaaa cttataaacc ttcatcccag caccataatt atttaaatca
      361 taggcatttt tcgaaattag tatatgtact caagtttcac tgcagattat ttatattttt
      421 tagagaaata aatttttcct attgacaaac acaaagaaag cggatacaat gcaattatgt
      481 cacatgacgt tatatgacgt tatgtgatat ctttacattt aatcagttac ttttattttg
      541 gataaaatta acatttttaa aattttaagg aggaaatgtt gatggatgta gcaaaaacgg
      601 ttaacgcaat tgtagaaaaa aatgatggag agatttctgg aatttatttg gtggcttgtg
      661 gcggttcgct ggtagatatg tatgtctcag attattttct taaagccgaa gcatcgaagt
      721 gtattacagg cttatataca gcgaacgaat ttaaccatgt aaccccaaag caattagatg
      781 ataaatctgt tgtcattcta tgttctcatg gaggaaatac agcagaaacg gtaaaagcag
      841 gagaagtcgc tcaagctaga ggagctgtga ctgttggtct gactcataat aaaactgcgg
      901 atttattaaa ggtttctgat catagtttcc tttacgaatg gggagatgat gcaaaaatag
      961 tcaataatcc tatggccatc attcttgatt taactgtatc tttggtcaat gttaccgaaa
     1021 catttgaaca ttacggtaaa ttcagagaag gcatgaaggt tatcgatacc gtaattgaaa
     1081 atggcattaa gcaggtgcag gatcgtatta aagtgtttgc tgataagtac aaagatgaaa
     1141 caatgtttta tatcttaggt agtggaccat catatggaca tgcttatggt ttttcaattt
     1201 gctctttgat ggaaatgcaa tggcttgatt caacttctgt tcattctgga gaattcttcc
     1261 atggaccttt tgaagtaaca gacaaagata cgaattttat tctcttgatg agcattggac
     1321 gcactcgtgc tttagatgaa cgggcaaaac gattccttga taaatatgca gaaagagtag
     1381 aaatcgttga tgcaaaggaa ctaggattgg atatgatatc agatgaagtt tctgaattct
     1441 tcaatcccat tttattctac agtattcttt cagaatatcg ttcagcttta gcagatgtac
     1501 gcaatcatga tttagatgtt cgtcgctata tggggaaagt ggagtactag gaggattaaa
     1561 atgaaggtat taggcttagg tgataacgtc gtcgataaat atgaaaatct gggaattatg
     1621 tatcctggag gaaattcatt gaattttgca gtttttgcaa aaaaactggg acatgaaagt
     1681 agctttatgg gagtttttgg ctcagatgag gaggcagctc atgttttatc agcaataaaa
     1741 gaaataggac tggacaacag tcactctcgc tttgaaaaag gcgagaatgg ctgtgccaga
     1801 gtgaagattg atgagggcga ccggattttc cttggcagca attcgggagg tgttactcgt
     1861 gaatacccga ttcagcttac tgaagaagac agagaatatt taaaccagtt tgaattgatt
     1921 catatgggat tatatagcca tgtcaatcat ctattagaag agttacaaaa tgtttctgga
     1981 aagatttctt atgatttttc tgacgacttt acggaagaag aggttcagcg ggcaattgat
     2041 aaagttgatt ttggcttctt ttctatagaa gacttttccg atgaagaagt aaagcagttt
     2101 ttgatgaagc atttttgtga gcgaaatgat gtattgattg caacccgagg agaaaaaacg
     2161 gctattggtt atgatggagc aaagttttat gaagtgaacc ccgtgctaga agaaccagtt
     2221 gatacaatgg cagcgggaga ttctttctta acagctttcc tactttattt tctcgaaggg
     2281 gacgatatag tcaccgcgat gaaaaagggg aatgaatttg ctgggaagtc ttgtctggtt
     2341 gacggttcgt tcggctttgg tattcattac gattgagctt gttttaaatt cctgcatttg
     2401 ataacctaaa gaaagaaatc ccggtaaagt ggaggttaaa aaaatgctgg atacaaatgc
     2461 acaattacca ttgtacttac aattaaaaga aatattaaaa aataaaatca atacagggga
     2521 gtatcccgag aatacgaaaa ttcccgctga atcagagctg atagaaagtt atgaggtcag
     2581 ccgaatcaca gtgagaaaag cagtagaaga actggtagaa gaagggtacc tacttaaaaa
     2641 acaagggaaa ggaacttttg tcaaccggca taaagttttt cgtaaaattg aatatattat
     2701 gggtttcagt gagagttgta aatctaatgg ttttacaccg actagcattg ttttggaacg
     2761 caagatcatt ccagcaactg aagggatggc aaaaaaactc gatatcgctc aaggtgatga
     2821 tgttttatat atccagagaa agcgaatggc ggataacatc cccattatgt tagaaaacaa
     2881 ttatttttct tatgaaagat atagtttctt aatggaggaa gagctgactg attctttgta
     2941 tgaagtactt aaagtccaag gtaccttagc catcaatcct ggggaaactg cacttgaaat
     3001 tgtcacagct gatgaccaaa ttgccaaaat aatggaagta gctgttggga cgcccttttt
     3061 ctttatggat actttaatca acgatcaaaa caacatacca attcatgtag gtaatcaata
     3121 ttacctggga gaagtatatc gtttttactt atgatttcct gtttggattt caaggagtag
     3181 attttattta tgaagcaaat attgcggtca aaaatgattt ttgatggaga gcagccacct
     3241 ttcaaaggtt atattgtaat caatgaaggg atcattgaaa aggtggaaaa caactggaat
     3301 tacagtgatc tattaaatga aaacacggaa ttaattgctt atgatgatca attcgttatg
     3361 ccagcaattc atgataatca tgtatttttc agtggttatt tggctatgaa tgctggcata
     3421 gatttaagcg atacatcttc agctaacgag gctgtagagc gtattaaagc tgtaatgaac
     3481 cataaaaaag cagatgaacc aatttatgct catggctggg atgctgccct gtggaaaact
     3541 gacccatcag aagagctatt gaatgaaata aaccactttg gtccaatcac agcgatcgac
     3601 aaaaatcgca gccattgttg gatgaatgct attgctaagc agacttatgg ttttacagaa
     3661 aaagaaacca gtgctgaaag tcgagtatta ttaattaagg aaatgctagc aaacaaaaag
     3721 cttttagcag aggtttacgc tgaatttgaa gcattactgc tttcaaaagg tgttatatca
     3781 atgaaagaaa tcgtttttga tgacgcagaa tttctttcta atatacctca gaggaaaata
     3841 cttagtaacc tatatgtgca ggcagtccaa gaacctctac agcatgaact tctatatgag
     3901 tataaggaaa aagttttccc tgaaaaggtc cgctttggtg gagtgaagat catggtagat
     3961 ggtgttgttg cggatagaag tggagatatt tacggtgttt atcagtcagg agtagcttca
     4021 cccgagattg actatgaggc aattaaagca gaagttgctg cattcaatga agtagagatt
     4081 ccttgctgtt tgacaacgga aggagataaa gcgggttata aagctgcgtc aattcttgtc
     4141 gaatttggaa agcggttacc tgaaggcgtt tttaattcga ttagtgattt ggaaatggtt
     4201 actcaaaaga cagcagaaaa aatgaacgaa ggacaagtag tagcagaggt atatccacaa
     4261 attctcggct tgaatcaaac gttagaagag gcttacatgt caactgttat aaaaaatgag
     4321 tcaagtccac aatcttgtgt aaaagttcaa cctcatcaaa agcacaatgt tttgcgttgt
     4381 gaacctaact gccatgcctt tctttctgag gagagaggtc aggagcagta gcgcagcgac
     4441 tcttgatgtc tctcctcaga aagaagagac tcgcacaggt tcatggtgct aaatgttatt
     4501 cgaattaaat tctaaatatt ttcgtggaga agccagccac tcagtgtctt tttccatcaa
     4561 aagtgttcca attatccgag tgatggaatt gatatttgga aaaatccgaa tcactcgttc
     4621 tctccggcga atctcttcgt tcaaccgttc caacacattg gttgtacgaa tccttcggcg
     4681 gatattttca ggaaaagaca aaatttgaat agcatcttca aagccgtttt ctaaaatgtc
     4741 gcaagcagca cttaattttg aatcacaacc atatttttcc aagaagtgct cttttctttc
     4801 acgtgtcaat tcaagttcag aggctttaaa gatactcttc aactcgtctt ttacatcaga
     4861 agaaactttc tttggcagct tatcaaaaat atttctcaaa aagtgggctt ggcatctttg
     4921 ccagctcgca cctaaaaagt tcttgcgaac agccttaact aaccctttgt gtgcatcaga
     4981 aatgaccagt tttacaccga acaaacctcg ttgaacgaac gattgaaaaa cggtatccca
     5041 attatcttcc gattcgccgt cttgaacatc aaaaccaaga atcgtacgtc gtccatcaga
     5101 atcaatgccg ataccaatat atacaccttt tgagacaata cgatgatttt cccttacttt
     5161 catatacagg gcatcacaca tgaaaaatgg atattccgag ccttctaggc tccggtttct
     5221 ccactcaaaa acaaatggat ccaactgttt catcagattt gaaacgaatg atttagaaac
     5281 agtcgcacca tccgttagca gttcaactgt tttagttact ttgcgagtgg agacaccaga
     5341 aatgaccatt tcaatcatgg cagcgatcaa agctttttcg cttcgctgat atctttcaaa
     5401 gatagtagga ctaaacttac cgtctctggt tcgtggaaca tgaagttcta acgtaccaat
     5461 tcgagttgta aacgaacgat cgtagtaacc atttctttgt gattgccgtt cttctgaacg
     5521 ttcataggca tttgcctgaa tatattgatt tcgttgttct tccatcagct gattaaacat
     5581 gatagtcata agtgtttttg cggtatcatt cgcaccgctt tcgtttataa ttgcttgaat
     5641 ttcttcgctt tcaaaagtaa aatgtacttg agtcattgat atcagtccta ttcgagtatg
     5701 ttttctgcaa aaaacattgt actcctaact gagatcaatg gcttttttga tgcttc
//
LOCUS       DACDMM010000148         5746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDMM010000148 DACDMM010000000
VERSION     DACDMM010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.149"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(342..698)
                     /locus_tag="IWD16_002300"
     CDS             complement(342..698)
                     /locus_tag="IWD16_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9089298.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(712..1122)
                     /locus_tag="IWD16_002301"
     CDS             complement(712..1122)
                     /locus_tag="IWD16_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9089299.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1469..3655)
                     /locus_tag="IWD16_002302"
     CDS             complement(1469..3655)
                     /locus_tag="IWD16_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9089300.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            3873..4556
                     /locus_tag="IWD16_002303"
     CDS             3873..4556
                     /locus_tag="IWD16_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP9089301.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4637..5356)
                     /locus_tag="IWD16_002304"
     CDS             complement(4637..5356)
                     /locus_tag="IWD16_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9089302.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
BASE COUNT         1593 a         1111 c          922 g         2120 t
ORIGIN      
        1 gatagaatca agcttcgcaa gtggttcttt tttaacaact cagttatctt atcatgtcat
       61 ttctaacatg tcaacaacta ttttatatgt tttttaaaat actattttat tgctttgcta
      121 cttaaaacaa cagaaatgaa tatatcatgt aaatttacac atgtcaatat atttttaact
      181 ctatatcccc acctagattt gaattttgac cacataaatt gagcatgtct ctatagtaga
      241 aaaataaagt caaaaaaaga acacataaca cgcttgatgc taagtgttcg actgttacaa
      301 ctgtatgaaa tgctcagtga cttgattaaa acagttaaag tctatttatt taagataagc
      361 tgttccatca actgctcatt gtatccaata aatagaactt catcgataat aatcaaagga
      421 cgtctgagga gattggtatg ttgtgtgcat aaaaaaatca gttgtttttt tcctacacag
      481 gaaaggtcaa tcgcaatgtt gtgaaaagcc tcagaccaag tcgcaaatag ctgttctgct
      541 tgcacttctt caaaactgca taattcttct gcttcttctg tacttagatc gcgttcttct
      601 aagtcaacaa attgataagg caattgatgg tgttctaacc attgtttcgc cttcagactt
      661 gttacacagt gcgctgatcc atagactttc acttccacga aggctccctc cctatgaatt
      721 taagatgtta ttgttttgta aatagatcag atttctggca acacatcgaa tttcacgagg
      781 gacaaatgac cggatctcat cttcattaaa tccgattagt attctttttt catcaatcaa
      841 aataggtcgt ctcaataatg aaggatattc tttgaataag acgatcattt gctttaaagt
      901 taattcgtta aaatctatgc ccagcttttc gtaaattttc gaccgagtgg aaataatatc
      961 ttgaactcct ccttcagtcg ctgccaagat ctttttgatc tcactttctg ttaacggttc
     1021 ggcaaaaata ttcttctctt caaattcaag ttgattcgct tgcaaccatg ctcttgcttt
     1081 tctacaggaa gtacagctag gcgaagtata tacttttatc attttctcac tcatttttaa
     1141 ttataatcca attctacagt aatttctttt aaaataccaa ttattagcta ataatatata
     1201 aaaaaaaatc aaaataactg attatttacg tttgaataat gagaattttt ctatatattc
     1261 tctattttga taatagtatt ctcattttca aaataaataa gggaggtgtg acaaaattct
     1321 tgtcacacct cccttttccg aataaactgt gttccagaaa tagcttcttc gaaaataagc
     1381 tgaaatttca caaaaatttg agaagcaatt ttcgtacatt ccttcttctt tcttgaaact
     1441 gaccacttct gtcacaccct ctcttctatc actttccttt tttcacacga ctcccggttt
     1501 ctttgataaa tctcgatact ttcaattttc ttgaattttt catttgaggt acacatagat
     1561 acagacgatt tcttgctcta gtcatcgcaa cataaaatag tcttcgttct tcttctatcg
     1621 tcgtcactgg ttctgctttt atttcattaa agtcgattac tttcttttct gtcgctccgt
     1681 tataaggcaa acttccttcg ttacaaccaa tcaaaaagac atttcggtaa ctaagacctt
     1741 tacttttgtg aatgctctgg atacatagtg catcaagttt tccttgtctg taagcaatca
     1801 agcgggtttt taattcttta gctgtcgctt gggtttcttg cgataaggct tggagtcttc
     1861 tgaaagtagc atgctgcttt acttcttctc gtatccattc ttgatcactc tggtcgattt
     1921 ccttagctat acgtggcaga tgaagatagc tttgaacaag cgaatagatt tcttgatgct
     1981 ggtctgtttt acgcaacgct tcctcaaact ctcggatttc tgcagatttc gattttaaat
     2041 tggggcttat cgacaaaata taatctgcaa cggctatatt ttgatattta ggagaaaagt
     2101 attgatcgca ttcacttttt ttcaggtagc agtctgccat cagctgccgg agcaattgct
     2161 ggtaggcctg atttttcttt tgcagattca gtgctgtttg ccaatgaaca attttttgat
     2221 agaaatgttg gaaagacttg ataccaaaga taccttcata tggactatct gcttccagta
     2281 caaaaggaat attcgttttc agcagagatt ggacaagctg ctgcgtttgt gaagctgatc
     2341 ggtataatat gattgattcc ttatatttcc catttttttg aataaatgtc tgaatttgtt
     2401 tgattagcca ctgactttct gctttagtat cagaaaacaa ataggcttcc ataacacctg
     2461 cttctttttg tgctggaatc atcgcctttg gtatccgatc atttttatta tagctgatca
     2521 atctatttgc tccttggaca atagctgccc gagaacgata attagtcaat aagaaaagag
     2581 ttttggtagg atattctttt tcaaagttct tgatgtatgc tggctctgat cctctgaatc
     2641 catagatact ctgatcatca tctccaacaa caatcaactt gatcctgttt tcctttgtca
     2701 tccgtttcac gatttcccat tgaagtggat tagtatcttg aaattcatca atgaatagat
     2761 aatcatattt ttggccgata taatgctgga tctcctcatt ctccaaagcg cttttcagat
     2821 tttggagtac atcattgaac tctagcaatt gctggcttct tttttgttct tggagttccg
     2881 tgagcaatcc atacagtcgt tctgatatcg gctgtcgtgc attcttatca atggaatcat
     2941 atttttcttt aactaactga accatttctt ctactgaaag gttcatattc tgccaatagt
     3001 ctaaccgaga agcaatcgtt ttttctccat attgatcaaa tatataaaga ttatatttac
     3061 gtatcagctg ttgcaaaaca gcattcactt tttgtgcagt atcctttgaa aagtctcctt
     3121 gtattccttg cattgctact tcaggaaatt ttcgacaaat ttcattgtat atcctaaaaa
     3181 agatactatg gaatgttccg atatggacag cggtttttct ctggctgaaa ttatctactc
     3241 gtttttttag ttcatttgca gcgttccttg taaaagtgat cattagtatg cgttcttcgt
     3301 ctacttgttt ttgcgtcatc agataaacag cttttgcaca gattgtttgc gttttgccgc
     3361 tcccagctcc tgctatgatt cgaagatgtt tcttcatatc atatgtgata gctgcttttt
     3421 gttcttgatt gaagcgatta taggaacgtt tctccatcat tttaaagaaa gagctgtttc
     3481 cttcactttc ttttacttgt acttcttcct caataagtcc gggtgcttcg ccataagccg
     3541 cttcataggt agcaatgatt tcttcttgta gccgaaagac ttgttgaagc tttttatact
     3601 tgttctccca gcgtttcaat tctttttgta atcggagaat ttctttttca ttcaactgat
     3661 tgatttcctt cccatctttc ttctttgaat ttgtttattt caagttggtt atttaaagtt
     3721 tcgttgtcta ttttttcttt atactaacag acctttctaa aaatgaaaat gggaagcgat
     3781 atgattttag ttttctgaaa atttttcctt tttttcaatc gtttatgcta agataaggaa
     3841 gacaaaaata ttcataaaag gtgatgaaaa aaatgcagac ttcctttcac gaggggcgta
     3901 caataggact tttgctgaca tttatcggag gagccatgga cagttatact tatatccaat
     3961 atggtgcttt tgcctctgct caaactggca atatcattct atcgatcatc caagcatttg
     4021 acggagaatg gctaagtgtt ggtaaaaaag tattgtctac ccttttcttt ttcttaggga
     4081 ttcttctaac aaagtatcta attgattatt ttagaaaaaa agaaaaacat ttctggcgct
     4141 tgtttgttct atattatgaa gcagtgattt ttttctttgt tagtttgaca cccgtccatg
     4201 agcatcccgc attagtaacg attttgattg cttttactgc atcgatccag tgggtagctt
     4261 ttgataaaat caacgggcgt gcctacacta atttatttac aactggaaat ttaaaagggg
     4321 tagcaacgaa cttatatgat tattgtattt ctaaagaaaa agcagatttt gatcgatttt
     4381 tccactatct aagtgtcgtt cttgccttta tcgcaggagc tattgtcctt gttttctgtc
     4441 atcatctgtt tggcgcccat tcgattttga tcgtttccat tttatttttg atcatggccc
     4501 tcactcagac atttcattta tggcaatttt atcatacgaa tcggttcact atttgatctc
     4561 aatgaatcaa aaaaactaag caagatgctg ctcacagaga aatatctgcg agtgagcaat
     4621 ttgcttagtt tttatattat ttatttgtaa agattccctt tgctttcggg ttatttgtag
     4681 tgtagcggat cgactgggca tctgcctctt ctgtcatttt ctttgcccgc atcttgattt
     4741 cttgtacgat ttgttctcca catgccggat cggctacgaa atcatatttg tccccatcga
     4801 tcactatttt gggacatata tcaaactgtt cataccactc ttcgtatcgt cgattgagtt
     4861 ctttatagta ggaatagagt tcaggatctt gttctagctg ttcatattcc cggcctcttt
     4921 ttttgattct cgctaacata gtttcaaagg atacacgaat ataaaccagt agatctggtt
     4981 tcttttgtgg cgaaatttct tctaactcct tcagcatcgt ttctaaaaga ccttcatact
     5041 gctttacttc tacttcagaa acacgcccta aatctgcatt caaatgaaac agcatactat
     5101 cttcgtagat ggagcggtct agtacgttgt catcatggct gaaagcatct ttgattgcca
     5161 aaaagcgttt gttcaaaaag aaaatctgta ataaaaaagt atacttttct ggattagaat
     5221 aaaatagcgg caatacttca ttatcttcca cgttctcata aaaaggtttt gtttctaaat
     5281 cttgggcaag catttcagtt agtgtgcttt tccctgcacc gatcgttcct gcaacaatga
     5341 tcacaattat cggcatcctt cctgtaataa aatctgttct tcattttact acttcattta
     5401 tagaaataaa agccttgact ttatctgctc cttaaaagct acttctctaa aagtagccaa
     5461 aagtaactcc tattcatgaa aataaaggct gtgacaaaag tcttgtcgca gccctttttt
     5521 caaataaaca gtattctcct gccaacacct cggtcacaag gcaatattca taaaacatga
     5581 aaagctgttt tttaacatta ttccttgtaa ctcagagcta aacgggagac tcacaacctc
     5641 tttatgctct caacatttta tctgttttta ttcttctatt gtacttgatt tgcgtacaac
     5701 taactgccag tgcagattat gagcggtatc attgtcttct actgcg
//
LOCUS       DACDMM010000149         5657 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDMM010000149 DACDMM010000000
VERSION     DACDMM010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.150"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            170..1162
                     /locus_tag="IWD16_002305"
     CDS             170..1162
                     /locus_tag="IWD16_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9089303.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            1222..2643
                     /locus_tag="IWD16_002306"
     CDS             1222..2643
                     /locus_tag="IWD16_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP9089304.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            2636..4135
                     /locus_tag="IWD16_002307"
     CDS             2636..4135
                     /locus_tag="IWD16_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP9089305.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            4174..5568
                     /gene="uxaC"
                     /locus_tag="IWD16_002308"
     CDS             4174..5568
                     /gene="uxaC"
                     /locus_tag="IWD16_002308"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9089306.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         1857 a         1048 c         1163 g         1589 t
ORIGIN      
        1 taattaaatg agcattcccc gctttcaaga atagcataat taaaaataaa aatccaagta
       61 atttttaacg tgtgcaatct tttcatatca aagatctatt gacttatcgt agttatcgta
      121 atatgatgaa tgcgttaaca aaaagtgaaa aagtaaggaa aatgatggaa tggtaacgat
      181 caaagatatc gctaaagcag ctggcgtttc tcatacgaca gtttctcgtg ctttgaataa
      241 cagcagttta ataaaaaatg aaacaaaaca aaaaatacga caactagcta aggaaatgaa
      301 ttattcgcca aactatagtg caaaaggttt agtgaatcaa aaaaaatatc taatcggact
      361 atttttctca agtttgcata aaggaacttc atcaaatttc ctagcagatt ccattgcagg
      421 catccattca gtattagaca caaattacag tttatctgtt gaaagtatcg atgttgttga
      481 tttaacagaa atcaacctgc aacgctacga tggactgatc gttatgagtc aaagtgatga
      541 tgatcagcct ttcatcgact atctcaaaga aaaagaattt cctttcgttg ttgtcaatcg
      601 tcacatcaat gatccagcgg ttatcaacgt cactgccgat gatgcggccg gtgttatgca
      661 ggcagtagat tacggtattg gatgcggaca tacgaagatc gcttatattg gcggaaagaa
      721 aaattttcgt tcgacaaaag aacgggaaaa aggggtattg aaaagtctaa aaaaagcgag
      781 catccctgtc aatctagcct attttttaac aggagattac agcttagaaa gcggcctcca
      841 aaacatggaa tacctattat cactttctga cctgcctact ctggtttttt gcggcaacga
      901 cgacatagcg attggtgcat tgagagctgt acatttagct ggcctccgtg ttccccagaa
      961 catttctctg atcgggttcg atgattctcc agtcgtctcc tatctcaatc cgccgcttac
     1021 caccgtacgt aagcctttga aagaaatgtg tcaagaagga acaaagcttt tactagattt
     1081 gatcgaaggc aggacgatcg aacaatctaa aattgagctc tccaccttgt tgatccttcg
     1141 tgagtcgatc aattacttgt aaactacaag taattttata taaaaatgtt aacgtgtgca
     1201 taacacagaa aggaagttta tatgaaaaaa ctgacaaaag aattagtgaa aaaaaacgaa
     1261 gcacctgtaa aagttcttca gtttggtgaa ggcaatttta tgcgtggatt tattgattgg
     1321 cagattcagc agctgaataa ccaacaattg tttaaaggta atgttgcaat cattcaacct
     1381 ttagaacaag gtcttggaaa tatgatgaac cagcaagata atttatatac ggtcatttta
     1441 caaggactca gcaatggcga aatcgttgat acttccgaga ttatcaccgt agtagaacaa
     1501 atgatcaatc cttatgatga atgggatgct tatctatctc tagcagaaaa tgacgacttg
     1561 gcctatatcg tctccaatac aacagaggca ggaatccaat tcgtggagac tgataattta
     1621 acagatactc ccccacagag cttcccgggc aaactgacag cctttttata ccgacgtttc
     1681 aaattaaaca aagctggctt taccatcatt ccttgcgaac tgattgacag aaacggcgaa
     1741 cgtctaaaag aaatcatttt acgctacgca gaaaaatggg atttagaacc tgcttttgtt
     1801 gagtggttag aaaaagaaaa tgttttctgt tgtagtttag ttgatcgcat cgtcccaggt
     1861 tatcctcgcg aagatgcagg tgttttcaat caacggcata actatgaaga tcaattgatg
     1921 gtcaaagcag aaccattcat gctttgggtg atcgaagggc ctaaagaatt ggagaatcaa
     1981 ctaccattca aacaggctgg cttgaatgtc atcatcacag atgatatgac tccttatcgt
     2041 gaacgtaagg tgcatttgct taatggaccg catacagcga tggtccctct tgctttgttg
     2101 gcggaattgg aaactgtaga agaagtaatg aaagatcctt tattctccgc atatgttgat
     2161 cagttattca atctagaatt gattcctatg ctttctttac ctaaagatga attagccatt
     2221 tatgctgacc aaatcaaaga acgtttcttg aatccttttg cccatcacaa attagaagcg
     2281 atttcattaa atagtgtttc aaagttcagc acccgtctgc ttccagtgtt caagaagtat
     2341 atcgaagaac aaaatcaggt accaccgtta atcactgttt cactagctgc gctacttctt
     2401 atgtatcgag gcgatcaagt aaagccacat gatgatgaaa agacgataag cgaatttact
     2461 gatgcttggt ctgataatgg aacagctatt cctcgattat tgcaaaacgc tgcgctttgg
     2521 ggagaagatt tgtctcaaat accgaatgtg acagatacag ttcaagaaat agctgcaaag
     2581 atcgatcaaa aaggtgtccg gtcagtatta caagaaatga aaggaacgat ggtccatgct
     2641 tgaaaccctg caaccaacaa tgaaattaaa tcgacgagac agtgtggtcg tcgcattaac
     2701 ccctattcca aaacaaacac aactgacgat tgatcagcaa acgatcacca ctcttgaaga
     2761 tatcccgcaa ggtcataaga ttgcattggg tgatttgaaa gctggagaca acgtgatcaa
     2821 atatggctat ccaatcggtc atgtgacgac agacgtaact gccggtcaat ggctgcacac
     2881 tcataatgta aaaaccaatc tttctggtga attggattat cgatatgaaa aagatgtcca
     2941 tccaagtcat tacctttttg agaaccgaac atttcaaggt tacttgcgaa aaaatggaaa
     3001 agtagggatt cgtaatgatt tgtttttagt acctaccgtt gggtgtgtga atggcattgc
     3061 tgaactgatc gtcaaacagt tcaaggaaaa acatccagat ctgggacagt ttgaccatat
     3121 tacgatttta aagcatcctt atggctgttc gcagcttgga aaagatcatc aaaatactcg
     3181 agaaatttta gcggacgcag taaatcatcc caatgccgga ggagttttag tatttggatt
     3241 aggttgtgag aacaatacag ttcctgaatt caaaaaaatt ttaggtgaat atgatgaaga
     3301 acgagtgaaa tttctagtgg ctcaagatgt ttacgatgag atcgaacaag gtgtggctct
     3361 attagaagag cttttaacag cggcggaaaa tgaccagcgc atatcagtcc cgctctctaa
     3421 gttgaatgtc ggtttgaaat gcggcggttc tgatggtcta tcgggaataa ctgctaatcc
     3481 tttacttggt gcattttctg actacttgat tgctcaagga ggcagcacga tcttaacaga
     3541 agtgccagaa atgtttggcg cagaacaagt attgatggca cgagcggaaa atgaagaagt
     3601 ttttgactca atcgttgact tgatcaatga cttcaaacag tatttcttgt cttacgggga
     3661 accagtttac gaaaacccat ctcctggtaa taaagcaggt gggatcacta cattagaaga
     3721 taagtcttta ggctgcacgc aaaaatcagg aagctctcct gtcgttgatg tgctacaata
     3781 tggcgaaaaa atcagaaaac cagggcttag tttattgcaa gctcccggga atgatcttgt
     3841 agctgcctct gctcttgctt catccgattg ccagttggtc ttgtttacca caggaagagg
     3901 gactccgttt ggctcatatg tcccaacggt caaggtatct acgaatacaa ctctttttga
     3961 acgcaaaggt cattggatgg attttaatgc aggcgtctta ttagaacaac caatggaagt
     4021 aatcttagaa tcgtttgttc aaaaaatcat cgctgtagct agtggagaag aaacgaaaaa
     4081 tgaacaaaat gacgttcgag aaattgccat ttttaaaaac ggagtaacac tttgattttc
     4141 ataaaacagt ataaaaagaa agaaggaaga tttatgtttt tatctgatga ttttttatta
     4201 aaagacgatt gggcgaaaaa gctttaccat tcctacgcaa aaaacatgcc cattattgat
     4261 tatcactgtc acttagatcc aaaagaaatt tacgaaaaca acaatttcag caatctgaca
     4321 gaagcttggt taggaggcga tcattacaaa tggcgcttga tgcgagcttg cggcgtgcct
     4381 gaagaaaaaa tcacaggaaa tgcttctgat tttgaaaaat ttttagcttg gtgtcaaacc
     4441 gtaccaaaaa ttatcggaaa tccgctttac agttggtcac atcttgaatt gaagcgtttc
     4501 ttcggtattg acttgttgat aaatgaagaa aatgcagaga aaatttggac acaagcaaat
     4561 gaaattttgg cagctcctga ttttcgccgt cgggaaatcg caaaaaaatc gaatgtagaa
     4621 gtgatttgta caacagatga tcccgcagac gacttgcact atcatcagtt gcttgctcat
     4681 gaagaaccag atttgaaggt tctaccttca ttccgtccag ataaagcact caatattgaa
     4741 aaagaaggat ttaatgcatg ggtcacacta ctggaagaag cagctgacca aacgatcacc
     4801 acctatcaag aactgataga agtcttaggg cagcggatag aatacttcca tcaacatgga
     4861 tgtcgtgttt ccgaccacgc tttagacatc ttacgctata aagaagcgga tgaaacagta
     4921 ttagaagaga tttttcaaaa agcacgagct aacaaactat tatcttcaga tgaaatcgat
     4981 gcttatctta cagaaacact gatacatttg acccacttct atcattctca taattggacg
     5041 atgcagctgc acatccatgc ttatcgtaac tgtaatacac aaatgttgga aaaattaggg
     5101 cccgataccg gctatgatgg aatgaatgat ctttctttga ctattcctct gcaaaaatta
     5161 ttgaaccgtg cagaagaaac ggatcagttg ccaaagacga tcctttattc attgaatccg
     5221 aatgactatc ctgcgatttt agctttgatg ggctgtttcc aaaaagaaca gaatggaaaa
     5281 ctgcagttag gttctggatg gtggtacaac gatactcgag ctggtatgcg cgatcaattg
     5341 acacaatttg ctgatggtag tgctttgggg aattttgtcg gcatgctgac agattcaaga
     5401 agtttcctgt cctacactag acatgaatat ttccgtcgtg ttctttgtga atttttaggc
     5461 gaaatggtca tgcgaggaga agcacctgaa gatgaacaac ttttaggaag tctagttcaa
     5521 gcgattagtt atacaaatgc taaaaattac tttggctttt ttgcctaatt gattaatagt
     5581 aacagtaacg aatcaaaaac aggatagtga aataacggaa tccgtttatc cacgttttag
     5641 agaagaagac ggaccaa
//
LOCUS       DACDMM010000150         5614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDMM010000150 DACDMM010000000
VERSION     DACDMM010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.151"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(59..2008)
                     /locus_tag="IWD16_002309"
     CDS             complement(59..2008)
                     /locus_tag="IWD16_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9089307.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="IWD16_002310"
     CDS             complement(2050..3108)
                     /locus_tag="IWD16_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP9089308.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="IWD16_002311"
     CDS             complement(3227..3682)
                     /locus_tag="IWD16_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089309.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="IWD16_002312"
     CDS             complement(4266..5069)
                     /locus_tag="IWD16_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9089310.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2076 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat attt
//
LOCUS       DACDMM010000151         5592 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDMM010000151 DACDMM010000000
VERSION     DACDMM010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.152"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            119..1537
                     /locus_tag="IWD16_002313"
     CDS             119..1537
                     /locus_tag="IWD16_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9089311.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            1608..3077
                     /locus_tag="IWD16_002314"
     CDS             1608..3077
                     /locus_tag="IWD16_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9089312.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            3087..3554
                     /locus_tag="IWD16_002315"
     CDS             3087..3554
                     /locus_tag="IWD16_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9089313.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            3590..4462
                     /locus_tag="IWD16_002316"
     CDS             3590..4462
                     /locus_tag="IWD16_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9089314.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            4543..>5592
                     /locus_tag="IWD16_002317"
     CDS             4543..>5592
                     /locus_tag="IWD16_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9089315.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSL"
BASE COUNT         1882 a          796 c         1137 g         1777 t
ORIGIN      
        1 aaatcggcga aaaattcctt cgtcgattta aaaatatttt tatgaaacgg gttgacatat
       61 gaaacgggtt gacatatatt agttgtgaag ataaaaacct tttcactgga ggaagaaaat
      121 ggagaaatta ttaattaaaa gcaataatca tcaactttac taccaaccta aggatgtttg
      181 ggtcggagat gttatgcctt acgggaaaga aggaaaattc tttatttatc atcaacgtga
      241 tactagaaat ccagttcctt ttggagaacc gtttggctgg gctttagcaa ctacgaaaga
      301 ttttgttcat tttgaagact ttggggaatc tcttttaaga ggggatgaca atgagagaga
      361 ccagtttatc tacgctggtt ctgtatttga ggcaaatgga aaggttcacg cattttatac
      421 agggtataat cgtgaattct tagcgcaggg aaagacatct caagtgttgc ttcatgctga
      481 gagtgatgat tttgttcatt gggaaaaatc agaaacagca ttggagttag aaccacaaga
      541 agggtatgat attcgaaact ggagagatcc atttgtcgtt tgggatgatg aaaaagaaga
      601 atatttgcta attttagggg cgcgaaaggg tcaggataaa catctacaaa ccggacgttt
      661 agtggaattt acatcaaaag atttaactaa ttgggaattc aaaggtgatt tttgggctcc
      721 gggattgtat accatgtttg aaatgccaga acttttcaag attggtgact ggtggtatct
      781 tgtctattca gaatactcag atggaaataa aatactctat cgaatgagtc aaagtttgga
      841 aggtccttgg attaagccta aagatgaagc ttttgatgga cgggcttatt atgcaggtag
      901 aacagcttat gatggaaaac gtcgagtcct atttggatgg gtaccaacaa aagatcaaga
      961 aaatgataaa aacaattatt tatggggagg tactttggtt cctcatgaag tctatcaaaa
     1021 agaggatttt tcacttgggg taaaacccat agacgaagtg tggaatgcat tcataaattt
     1081 acatgatatt gccgatgtag agatagaaac agcagacaca agagaagaag taacgcttgt
     1141 caaaaatagt ggaactttgt ttaatttaga aacaacaatt tcatttgatg agaatcttag
     1201 tgacttttca ctgagattat ataaaaacct tgagacagat gaatcttacg aatatcgctt
     1261 caatttagat gaggggcgtc ttacatttga taaaaaccct tgttaccctt ggtttcaata
     1321 tatggataaa ggacttatta gacctatatc tttaaagcct aaccaatctt ataaactaaa
     1381 agtgattgtt gatgacaata taatgacact ttatattgat ggaatcgctc ttaacgctcg
     1441 tgtacatggt tgttttggtc aagcaattac tattacagca acaaatggat caatgactgc
     1501 aataaatacc aagttttcaa atgaaataaa gaaataataa acgtcaatca tgtggcaggt
     1561 ctgaaaatca aacatgacct gtcacattta aaagggagga taaatgaatg gactacaaaa
     1621 aaattgccga agatgtattg gaaaacgttg gaggaaaaga gaatatctca agcgctacac
     1681 actgcattac tcgtttacgt ttaattctta aagacaaaag caaagcgaat atggaaatgc
     1741 ttgaggaaat agaaggtgca aaaggtgttc ttttcaatag tggacagtta caagtaattt
     1801 tcggaacagg tataattgaa aaggtttaca aagagttcgt tgaattgaca ggagctaagg
     1861 aagtatccgt ttctgaaatg aaagacgaag gagtcaataa ttcaggaaat agacttcaac
     1921 gagcttttaa agttttctcg gatatcttta ttccgatcat tccagctttt attggggcag
     1981 caatggtttt aggaattcgt tcgttgatta ccactcctgg aatgtttgga atggatggct
     2041 cattcgctga tcacaatgta ttcctaaatg atttatccaa atttctgggt attattgcaa
     2101 ccacatttga ttatttacca gttttagtta tgtattctgc aacaaaacgt tttggcggaa
     2161 accctatttt aggattactt gtaggatttg ttatgattca tccaggacta gccaatcgga
     2221 atgatttcgt catgggaact ataaagcctg attattggca tttatttgga ttaagcattc
     2281 cagcagttgc gtttcaaggg ggtgtttttc cagcaatttt gacttcttgg tttttagcaa
     2341 aagttgagaa aattacaaat aaatattctc ctcaagtact tgcttacatt ttagtcccaa
     2401 ctgtgacaat tgttcttgcc aactttgcac tatttttagt gtttggtcca ttggggaatt
     2461 ttatcggtaa tgttttaggt ggaatcattg atttcctata tatgaagatg ggtgcctttg
     2521 gtgcttttgt ttttgcaggc gcgttacaac cattagttgt aacaggaaca caacatgcta
     2581 ttcaaggtat tgaggcaaac ttagtcgcaa caactggatt caattatatc caacctattt
     2641 ggtcagtttc aattattgcc caaggtggcg gttgtattgg tatgtattta ctctttaaga
     2701 aaaaatctaa agatagagat attgcgattt caagctttat tccaacactg gtaggtatta
     2761 ctgaaccggc tatctttgct gttaatttga aatattcaat cgttcctttt gtttgctcgt
     2821 ttattggtgc tggttttggt ggagcattta tgaaaatttt tgatgtaaaa gccattggac
     2881 aaggattgac tgtattgcca ggtctgacta ttgctaatcc agttcttcct tatatatttg
     2941 gtaatctcat agcattcatt ttaccaatca tttttattct cgcttatgat aaatataaag
     3001 tgtttatccc tgaagctaaa caccgtaaat ttgcagaaaa aactgactca aaaaaattgc
     3061 cagttcaagt aaagtgagat gagagcatgt taaatttttt tagaaaaaat gaaataagcg
     3121 ttgtagcacc tgcaaccggt gtttttgaaa aactggagaa attaagtgat ccagtctttt
     3181 caaaaggaat gatgggacaa ggatttgcaa ttgatgcaaa aaacgaaacg attgtatctc
     3241 caatcgatgg ttttgtttca agtatttttc ctacaaaaca tgccttagga ataaaaacaa
     3301 aatcaggaat tgagatactt gttcatgtag gaatagatac agttgagcta aatggtgagg
     3361 gatttgatat taaagttcaa gaaggacaaa aagttaaggc tggggatatt cttatgacca
     3421 ttaattttgt agtttttgaa aagaatcaca agactaaaga tgtcattgtt gtttttccag
     3481 actaccaaga cgaaattttc attccgaaac ttgaagaagt aaaacaagga caagaattgt
     3541 ttgttcttgg ctaatgataa gtaattaata atagaatcga ggaaaatgta tggtttttgg
     3601 ggctattgaa gctggtggaa caaaatttgt ttgtgccatt ggagataaag atttgaacat
     3661 atttgaaaaa gtcagctttc ccacgactac acccaaagaa acaatgaatt tagttttaga
     3721 tttttttaat catcataaag atgatttaga aggtatcgga gtgggatcgt tcggtcctat
     3781 tgatattcat tgtgaatcta aaacgtatgg cttcattaca tcaacgccaa aacttgcttg
     3841 gcaggatttt gattttattg gaagtttaaa aaagcaactt aatattccaa ctgcttggac
     3901 gactgatgta aatgcctctt gctatggtga atatgtttct ggaaatggca gaggcctttc
     3961 aagtgttgtt tactataccg taggaacggg cataggaggt ggcgctatcc aaaacggaaa
     4021 cttcattgaa ggttttagtc atccagaaat gggacatatg cttgtaaaac gacacaataa
     4081 agacaacttt tctgggacat gtccattcca cggagactgt ttagagggct tagctgctgg
     4141 tccggcaatc gaaagaagat cagggaaaaa gggacaagaa gtgactgaaa atgacgttac
     4201 ttgggaaata gaagcttatt acattgctca gtgtgcatat aatacaacat tgatgttatc
     4261 acctgatgtc attatatttg gtggcggagt aatgaaacaa aaacacttaa aaaagaaagt
     4321 acaagataag tttattgagt tgatcaacgg atatgtcgct attcccgatg ttgacaatta
     4381 tattatgaca ccaaaattgt tagataattc tggtattgta ggttgtttag ctttagcaag
     4441 agacgttaaa gaaaaatctt aatttggaaa aaagatcgtc tcgactattt gtttggttga
     4501 tgcgatcttt ttttcaatta gtaattgaag ggacgcctaa aagtgaaaaa aaaaatgaaa
     4561 gatgaagtat tagagtatat caaaaataat ttaaagtatt acgatttttc tgctcaagat
     4621 attgcaatga aattttgtat taaaagaaat gtcgcttccc actatttgaa tcagttattt
     4681 tcagatggaa aattactaaa gaatgattct gttagaccag taatgttcaa gtataatcaa
     4741 caaaagccaa aagactgctt ttctaagttt attggagcag acatatcttt gaaatccact
     4801 atagataaat gcaaagctac tgtaatgtat ccccctaatg gcctcccatt aataataaaa
     4861 ggaaacagtg gcgttggaaa aagtttttta gcaagtttga tctatcagta tgctcttgat
     4921 agaaaagtaa ttcataatga cgcaaaattt gttgttgtga attgtgcaga ttatgcaaat
     4981 aatccagagc tattgtcagc agttttattt ggatataaaa aaggagcctt tactggggct
     5041 gaaaaagata ctgcagggat gctatcttct gctaatggag gatacctttt tttagatgag
     5101 gttcacaatc taagtgcaga aaaccaagag aaattgtttc ttcttatgga ttctcaaaag
     5161 tatcgtaaac ttggtgaaag tgtgcaatgg gagtatgcaa atgttcgcct tatcttagcc
     5221 acaacagaag ataaaaatac ctctttgtta gcaactttta gaagacgcat cccagctgaa
     5281 atcactctgc cagattacaa taatagaagt acatctgaaa aaattcaatt attatttgaa
     5341 tttcttaaag atgaagcaat tacgattgat tcaaaaatat tttgttcgat tgaacttttt
     5401 actgatcttt tatcgaaaac atttgaagga aatgttggag aattgaggaa tgaaatcaag
     5461 cttctttgtg cagaaggtta tttaggaaat aagagaaagt cttcaattta tttaggggac
     5521 aaactagaca gcggtttttg gatagatcca gaaattagta tagatttaaa aaaaatgttt
     5581 cttaaatcac tt
//
LOCUS       DACDMM010000152         5589 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDMM010000152 DACDMM010000000
VERSION     DACDMM010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.153"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(147..824)
                     /gene="nth"
                     /locus_tag="IWD16_002318"
     CDS             complement(147..824)
                     /gene="nth"
                     /locus_tag="IWD16_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP9089316.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(831..1529)
                     /locus_tag="IWD16_002319"
     CDS             complement(831..1529)
                     /locus_tag="IWD16_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP9089317.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1797..2981
                     /locus_tag="IWD16_002320"
     CDS             1797..2981
                     /locus_tag="IWD16_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP9089318.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2987..3430)
                     /locus_tag="IWD16_002321"
     CDS             complement(2987..3430)
                     /locus_tag="IWD16_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9089319.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3656..3943)
                     /locus_tag="IWD16_002322"
     CDS             complement(3656..3943)
                     /locus_tag="IWD16_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089320.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4114..5466)
                     /locus_tag="IWD16_002323"
     CDS             complement(4114..5466)
                     /locus_tag="IWD16_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP9089321.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
BASE COUNT         1466 a         1122 c          951 g         2050 t
ORIGIN      
        1 ttttatcgca ataaattgta ataagttacc atcctagcta ctgagtatgt acatttactt
       61 tttcatgcaa aaaagataaa cgaaacagct tttctccata ataacaagat aattcataca
      121 tttatttgca attaaagaaa ccacgtctac aggcgtggtt tcttttttag cgccttttct
      181 tttagacgca tccgattttt cccatcttga catattgata gcaaaggaca tacctcgcac
      241 ttgggatttc ttgccgtgca atgatatcta ccaaaaaaaa tcagtgtatg atgtgtcttt
      301 acccataatt cttgtggtac tttccgcatc agtgtttctt ctacttccat gaccgtagca
      361 tctagtttgc agatcctcag gcgttttgag acacgttcca cgtgggtatc tactgcgatt
      421 gctggtatcc cgaatgcatc ccccaatacg acatttgctg tctttctgcc tacaccagga
      481 agagacatca attcttctct tgacgttggc acttgcccat cgaaacgttc aatcagctgc
      541 tgtgcacacg ctttgatgtt ttttgcttta tttcgataca aaccaatcgt ttttatttta
      601 agaatgattt catcgattga agcttctgct aacgcatcgg gtgtcggaaa agaggcaaac
      661 aaatctggcg ttgctttatt cactgataca tctgtcgcct gggcacttaa gatcactgca
      721 atcaataact caaatggatt attgtgcttg agttctccat gtgcttcggg aaacattcca
      781 tacatcgttt ctagagcttc cattgttttt tgtttgctta acatattccg ctactcctcc
      841 aaccaatttt tcagtgagac tttcggcaat tcttcttgtt gagctgactg ttctacttct
      901 ctttgaagaa gctgctgttt tcttcgtttt tggtcttctt ccacttgttg ttttgtacgt
      961 agattttttc tttcccacga tagcaaaatg cggtcaacgt aattaaaact ataagcctga
     1021 tttaatacgg cttcccgcag tgctagcttc aaaatttccg gctgataatg gtcttcttct
     1081 agccattgtc cgatccgctg gaactcgatc gaagaaagcg ggcgtccaaa ttcttgttca
     1141 aataattgat acactgactg gatttctttt tcttgtgttt tttcttcgta ttttttttct
     1201 tgtgttttta aatactcacc taatagatca tagatggggt aaagattata tcgatctgct
     1261 tttttaccgt tgacaatcgt cgtttctatc ttgataaatc catttgttac gagacgatcc
     1321 agtatttgaa aaatctgatc ttgtttgatt cccatatcca tagcaatcat ctgcaaatca
     1381 gggaaagtat ctccttcaag ctgtgccata tgcaactgta gaacaaataa aaattcttca
     1441 gattgtagac cgatcctatg ataattttta agtaaaagat tagatatgac tgtttgtcca
     1501 gcgcttaagt aattttctaa attcaacatg atttctcctc cgctcaatca cagtatacgt
     1561 aaaaaaaaca tgggaagcaa gaacaggttt cttttctatt tacttcactg atcggtcaga
     1621 tttacaatgt cgtctacttg atttttgttt gttcatgttt ttgatgcgta tcttcacttt
     1681 tcggaataaa taaattaagt agatttcgct gttccttaga atgttgacaa ggaaacaaaa
     1741 ttactataca ctatttttgt ttccaagtta acattattgc ataaaggagt ttttacatgt
     1801 acagtttcac aaataaacat tctaaagttt ttggatacgt ctctgtctta ttcactggat
     1861 taggatttac tatcgtcagt cctgtacttc cgtttttaac gctgccttat tctcactcca
     1921 tacatcagca agctttctat atcactttgc tcatgtctgt ttatgctcta gctgccttct
     1981 tatctgcccc cattctcggt agcttaagtg atcattttgg gcgtcatccg atccttatta
     2041 tcagtttatt aggatcaagt atcggatatt tgctttttgg tttaggaaat tcgatatgga
     2101 tgttgttcct tggtcgtatc attgaaggat tgacggcagg agaaatctcc accttatatg
     2161 cttactttgc cgacatcacg gaacctaatg aaagaaccaa agtttttgga tggatgggtg
     2221 ctttagtcgg tataggaaca acgctgggtc ctattatagg gggtctatta gctgagttgg
     2281 ggaattctgt tcctatattt attggagcac tctttacttt cttaaatgcc gtttatggct
     2341 acacatttat gctagaaagc cttccaataa aaaaacgttc agtagattta agtttctccc
     2401 atgtaagacc ttttcaccaa ttacaacaat tattcaagat ttcatccgtt attcctttat
     2461 tgacagcagg ttttgctgtt tggttggcag ctggctctct tcaatctatc ttttctcaat
     2521 tttcgattga cgcctttcag tggaaggctg ggttggttgg tttaagcttc tctttgattg
     2581 gaatcttgga tactgtctcg caattgttca ttatgcctcg attgctcaaa aaattttcag
     2641 aacagcagat cacaagaata gggatgttca gtgaaatcct cgcctatcta ttcatcacat
     2701 tatctggaat tcttctactg ccgattttgt ttttatttgg gattatctgt tatggtttcg
     2761 gtgattctat ttttactcct gtattcaatg gccaactatc caattctgtc tcagataacc
     2821 agcaaggtct tgtaatgggc ggtacccaaa gtatacaatc tctatcgcgc gtcatcggcc
     2881 cgctaatcgc aggacagctt tatgctgcag caccatttct tccgacggta ttcggatttt
     2941 tgcttatgat ttttgcctat tatcactaca ataaaaaata gtagcttcat ttgctacttc
     3001 gtataaattc tctgtatcga ccgataaaac gaaatactgt gtcaaattct tcttttgaca
     3061 tattttcaaa tacagtagca tctcgctgta cccatttttg atggagttgc tcgtgtctat
     3121 tccttatttt ttgtccgatt ggcgtcaatt gaaaatagat ttctttttta ttctcttcgc
     3181 tttgataagt gtttatgatc cctctagcta tcagtttttt tgttagctta ctgatggctc
     3241 ctctagtcat gtaactagca ttcgctaatt ttgtcacgtt aggatcaggg attttcccga
     3301 tatgttcaat cacttctagt tcatttagag taaatcctgc aaattctttt tccatttctg
     3361 gtttattacg atgaataatc tcattagcta aatccagata ttcttctatt agcagttgtc
     3421 ttttatccat ttctacacct tctttttctt tcattatatt gtttttttgt ttcctattca
     3481 acaaagataa aaatattttg taggaaaacc caatctgaaa ccaagaaaaa aaggggttga
     3541 caaagcttgt cataacccct ttttctgatt aaaagatagt aaagagacag tcatcggtaa
     3601 ttgtgatgat tttttgtcat cacttaagga tgagcggcct tttcaaaaac tgtctttaaa
     3661 gatcatcatc agtttctagc acatatggct gaccttcata tgtgtactct gtattcaatg
     3721 tgccgtcatt ttcatctgag tagatgcgtc tactgaaagg aaaacgatga gaaacattat
     3781 ctttggcgtc atttgctggc atcgtgtaat attcctcttg cgtttcattc aactgagaga
     3841 gtacttgatc tgccaactca tcaatattga tcgttaattt cttcgaagca cttttataat
     3901 cttctaaaaa tgcatcttgt tggacaaaat attctttatt catcttcttt ctcacctttc
     3961 ttttcaaaat caaataaagc tgcttgttct tttatgtaac agctttatct ttgctgctta
     4021 ttgtgacaaa aaattcgaag aaagacaact aattgtactt ttcacaaaaa aagacagagg
     4081 gtcgtgacaa aattgtgtca caaccctttt tctttattgt gcagtttcga tttcgcctag
     4141 cgctgcttgt gcaagtaggt tcaagtaatt ccatgggcgg tcaaaatgtg gttggaagaa
     4201 gaaatctgaa agtgcgagat cttctacagt cattttattt tgaacagcta aagacatggt
     4261 atttgctgat tgtgtaatgt cgtatttgga catgaactga gcacctacga tacgattcgt
     4321 tcctttttca tagactagct ccatcattac tttttcagta gttggcatga actctggacg
     4381 ataattatct tcaaatacag tcgcttggac ttctagatcg ttcaaagttg cgctttcttt
     4441 ggtcacacct gtcgaaccga tcgtccagcc aaataggtaa agtcctgaag ttccttgagt
     4501 accacgatac gccattttat gatctgtcaa gttatttccc acaagcaaac cttgacgaac
     4561 ggcatttgtt gccaaaggaa tatagttttg cgtgccactt ggattgtagt tgactactgc
     4621 agagtctcca gctgcaaaaa tgtctggatt acttgtctgc atgtattcat tcactttgat
     4681 tgccccgttt ggcagtgtat ctacttttcc ttcgatcagt tttgtatttg gacggaagcc
     4741 cacacataaa atgaccatat ctgtatcaaa tgtatctgtt ggtgtagcta cttgtttcac
     4801 ttttccttct tcatctgtga taaattcagt aacattttcg cctagcgcaa gagtaacacc
     4861 gcggtctgtc aattcttttt ctaagatatc tgtgaatggt ttatctagat atttattcaa
     4921 gatgcgatct aatccatcaa tcaatgtcac ttgttttcca gattcgacaa aagcttctac
     4981 taattcaata ccgatatatc caccaccaac aattactaca cgttttgctt ctttcgcttg
     5041 tgcaataatt tcatttgctt gattgtagtt tttgcatagg agtacatttt tcgcatcgat
     5101 tccactaatc ggtgggataa taggccatga accagttgtc atcactaatt tatcataaga
     5161 aaccgtttct gtttctcctg ttttcagatc ttttgccgtt actgttttat tttctgtatc
     5221 gacgtctgtt acatcgtgtt ccattttaac tgttgctcct aatgaagata attcttcagg
     5281 atttgagtag aacaagcctg ctgggtcctt tactactccg ccgacataaa gtgcgatacc
     5341 gcaagataag aaagaaatat tgtcatttct ttcatatact gtcacttctg cttctggatg
     5401 attttttaaa atacttttta cagctgcagt acctgcatga gtacagccaa ttactactac
     5461 tttcatttat ggtccctccg attaacattt tgtccttcga caaaactact ataacagaaa
     5521 taaaacggat acaacagttt aatgcttgtg aattcacaaa ggtttgtttg tagtttcatt
     5581 ccttttcaa
//
LOCUS       DACDMM010000153         5532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDMM010000153 DACDMM010000000
VERSION     DACDMM010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.154"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..985)
                     /locus_tag="IWD16_002324"
     CDS             complement(<1..985)
                     /locus_tag="IWD16_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAP9089322.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="IWD16_002325"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="IWD16_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9089323.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="IWD16_002326"
     CDS             1810..2964
                     /locus_tag="IWD16_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9089324.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="IWD16_002327"
     CDS             3179..4147
                     /locus_tag="IWD16_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP9089325.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="IWD16_002328"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="IWD16_002328"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP9089326.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..>5532
                     /locus_tag="IWD16_002329"
     CDS             5177..>5532
                     /locus_tag="IWD16_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9089327.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRG"
BASE COUNT         1669 a         1078 c         1252 g         1533 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc gg
//
LOCUS       DACDMM010000154         5445 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDMM010000154 DACDMM010000000
VERSION     DACDMM010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.155"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            224..1156
                     /locus_tag="IWD16_002330"
     CDS             224..1156
                     /locus_tag="IWD16_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089328.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1377..3140
                     /locus_tag="IWD16_002331"
     CDS             1377..3140
                     /locus_tag="IWD16_002331"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9089329.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3224..3682
                     /locus_tag="IWD16_002332"
     CDS             3224..3682
                     /locus_tag="IWD16_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP9089330.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3791..4474
                     /locus_tag="IWD16_002333"
     CDS             3791..4474
                     /locus_tag="IWD16_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9089331.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4621..5235
                     /locus_tag="IWD16_002334"
     CDS             4621..5235
                     /locus_tag="IWD16_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP9089332.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5251..>5445
                     /locus_tag="IWD16_002335"
     CDS             5251..>5445
                     /locus_tag="IWD16_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089333.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
BASE COUNT         1936 a          855 c         1119 g         1535 t
ORIGIN      
        1 cacgagtaca gaactggctg atcttgaatt tatttctttg agcaaagtat atacaatcaa
       61 aaaaatcgta ttggattttt ttaaagaaaa tgattatttg aaaaataaag aaatcattat
      121 cccagaattt gactcgcgga acatcttgct agctttagta cgttatgtca attgggaagg
      181 ctatgaaaat aaaaatccgt atgtagaaga agcttgtaac gagttgattg attatgtaga
      241 gactcatttt tttaaaagaa gatattcgga agatgaaaag tcctttatca tacgaggaat
      301 agagatcgca atcggaagaa aggcatttcc ccttggactg aaagaagaag ataagaaaag
      361 cgcagaaacg aagccgttat ttcaattagt ctgtcaagga ttggaagagc aaaaaggaat
      421 tctagactta caagaagaag atgtctatta tattttttct ttgttcaaca gtagaaatta
      481 taccaatgag aacatggagc tgttaagaaa agatttcgaa gttgtttata aaaattttgt
      541 tctcaatgac ctatcactga atgaattgat tttacagatt attagtatcg ctgaaatcga
      601 cgatgcagaa aattttattt tcaagcaatc ctttttacaa tttgtccgta cgttgtgggc
      661 agatggacaa gtatttttac cggaaaggat ctatcttcta tcagaaaaag aacaacttct
      721 gtacggaaaa attgtagaaa tattggaaga gtggaaaaag aaaaatcaaa tccaactaag
      781 atggaatgaa aatttcattc gaaaattcgt caaaagtctt tctttgatca acagtgaaca
      841 agcggggaga caggaaatag aaatttttgt tgtttcagaa agttcagtca aacaattttt
      901 ttatcgaacc caatttcatt tgtatgcgga agaaggcatc gctgaaataa atcctatttt
      961 atttcgttca ttagaagaat tgcctgatga atgtctatgt gcgaaaaaaa gagtggtttt
     1021 gtgcgatgct gctagctatc aaaatggatt agaaactgaa aagacaaaga tctatccaat
     1081 ctcattaaaa ggtgcaaaca tccatttgta tcatattgct caggaacttc acctattaaa
     1141 aaaacatatt tcttgatgag accttaatta ggagaccttc tacaaagctt tttaattgtc
     1201 tgtagaaggt ttttatttga gtcctgattc gtgcgaagat ctacttatat aacaagcttt
     1261 ctctgaaatt tttttaaaat gctattgact tagagtcaac tctaagtagt agaatagata
     1321 aagtgtaaca aatctatttt cattaaaaga agatagtacg gaggagagaa tgtattatga
     1381 gtacagtatt acatgtattg gatgcaatca atagccccgc agatgtcaaa aaattatctg
     1441 ttgaggaaat gaaacagttg gcagaagaga tgcgccaaaa aatcttgcaa aaggacagcg
     1501 atgtaggtgg gcacgttggt ccgaatctag gtgtagtaga attaacgatt gcgtttcatt
     1561 atgtatttga ttcaccaaag gataaaatcg tttgggatgt ttcccatcag tcttatggtc
     1621 ataagctttt aactggtcga aaaaatggtt ttgaaaaagg acattaccgt gaagtgagcg
     1681 gatattccaa ccaacatgaa agtaaacatg actattttac agtggggcac acatctacat
     1741 ctatcagctt agcaacaggt ttagctaaag cgagagatct gaagaaagaa tcaggaaatg
     1801 tggtagcatt tatcggtgac ggctccctaa gtggtggctt agcttttgaa ggattgaaca
     1861 atggtggtgt tttacaatcc aatctaatcg tattagtaaa cgataatgag atgtctattg
     1921 atgagaatca tggcagttta tataaaaact tagctgagct tcgtcaaacc aacggtaaaa
     1981 gcagcaataa cctatttaaa gcaatgggat ttgattatcg ttacgtggat aatggaaacg
     2041 atctggaaac ggtgatccgt ttatttgaag aagtaaaaga tattgatcat ccgattgtac
     2101 ttcacgtcca tacggaaaaa ggtcgcggtt atcagccggc aatcgataat aaaatgaaat
     2161 atcattggca tgtaccattt gatctgtcca ccggggaggc aaagcaagta acttctggag
     2221 aaacatttaa tatgatcatc atggattatt tggataagaa aatgcaaaca agtacagctc
     2281 ctattgccgc agtcaatgca gcgataccag gcgcctttgg actgaaaaca ttccaagaaa
     2341 aacatccaga tcattatttt gacgtgggga tcgctgaaca gcactctatc actttcgctt
     2401 ctgctttggc ttctcaggga gtacgtccag tcgtcttcca atcaagtact ttcttgcaac
     2461 gggcatatga tcaattatca catgatttag cgatcaacga gaatccagtg attattttag
     2521 tcggtggcgg aacgatctca gctagtgacc gaacacacca aggaacattt gacattcctt
     2581 acttatcaag tatcccgaac atcaaatatc ttgctccagc aacaaaagag gatatgatct
     2641 ctatgctaga ttgggcgttc gaacaaacag cgcagcctgt agcaatccga attcctacac
     2701 atggtgtaga acatggtaca gctatcgcaa atgattatag tattccagcc tatcaaacag
     2761 taaataatgg aggaaaagta gcaattttag ctttaggcgg ttttcttgaa ttaggaaaag
     2821 aaacagtaaa agcttttaga aaaaaaggaa tcgaagcaac gttgatcaat ccaggaacga
     2881 tcacagaaaa tgacgtagaa acattagata aattaaaaga aacacacgaa ttagtcatca
     2941 cattagaaga tggtagtttg tcaggaggat ttggcgaaaa tatcagtcgt tattatggtg
     3001 attcttcaat gaacgtatta aattttggtg cagagaaaga attcacggat agcgttcctg
     3061 tagggcagct ttatgaacgt tatcatttaa caccaaaaca aatcgttgaa gatagtctgc
     3121 gtgtcttgaa tattcaataa ttcattatgc tcaccatcca gataattgtt tatactagta
     3181 tctggatggt tttttcatta taaaaagcaa aggggtggat gttatgtatc agcaagtaga
     3241 agaatatgtc aaaaagttcc aagaaaaata tcctagttat caatcagaaa aattaggttt
     3301 cttttcattt ggggatacag aaaaaatggc agacgagttg agcgaactag ttgtaaaaaa
     3361 gataaagact ggtacatcaa gtggttacga tctgtataaa caagagaatg agcctttacc
     3421 aaaagttggt caattagacg tggtcctgaa tggaaaaaat gaaccagttt gtatcatcaa
     3481 aaatataaat gtgaccatct tgccttttag tgaagtcacg aaagagcatg cttggaaaga
     3541 aggagaaggg gatcggacat tagcttattg gcggaaagtc catgctgagt tttttaaaca
     3601 tgcttataaa gaagcgttga acatagaatt tacagaagag aagctggtcg tatacgaaga
     3661 atttgaagta attttctttt agtaagaaag gtctgctgaa agaaatagct cattattgtc
     3721 tcctgaatag aaaagggtta catttttata tgaagctgta tttttatata acattactgg
     3781 aggagatatt gtggatacac tggaacaaat caaaggcgga gtcgtcgttt cttgtcaagc
     3841 tttggaaaac gagccgctac acagttcttt tattatgggg agaatggctt tggctgcaaa
     3901 agaaggcgga gcagttggta ttcgagcaaa tacgacagaa gatatcaatg aaatcaagcg
     3961 aatcgttgat ttaccggtaa tagggattat caaaagagat tacgatgggt cagaagtgtt
     4021 tattactgcg acagaaaaag aagtggaaga acttttggag acgcaagcag aaatcattgc
     4081 actggatgca acttctcgta aacgtccgac agaaaaaaca actgctgaac ttgtccaaat
     4141 gattcacgaa cacggtcggt tagctatggc agatatttcg acttttgagg aagcgcttca
     4201 agcgcaagaa gatggttttg atcttttgtc tacgacttta gcaggctata cagaatattc
     4261 acgaaagacg cagacacctg attttgattt attgaatcaa atcatagaac aagtcgatat
     4321 cccagtaatt atggaaggac atactgactc tcctgaacaa gtagagaaag catatgaatt
     4381 aggtgctttt tcagtcgtag ttggatctat tatcacccgt ccgcaattga tcactaaaag
     4441 atatgtcgaa gccgcaagta aagcaaataa ctaaaaaaca cgcacggcac agattcttga
     4501 caaaaaagaa aaattgatag atagtttaaa gcagattcta agaagatgaa aaaagcatta
     4561 cttttcattt tgtcttcaag atctgctttt taacgaattt tatagaaaga aggcgaaacc
     4621 atgtttgaac ggagtccttc agaattgaca gaacgtgaaa attacaagtt gctgattggt
     4681 tcagtcatcc ctagaccagt agcagtagta acgactaaat cagcaaaaga tatagtgaat
     4741 atcgcaccct tcagttactt caatatcgtc agttcgaatc gacccatctt atccttggcg
     4801 atccagagaa aacaagatgc agtaaaagac acagcaaaga atattcttga gacaagagaa
     4861 gctgttatcc atatccttga tgaggacaac gtagaagaag taaataaaac agcagccaat
     4921 ctgcctaatg aggaaagcga actgtacctg actgacttga ctttaactga ttcagtaatc
     4981 atttcagtac cagcattgac agaagcaaaa gttcgttttg aaacatcctt atatgaacat
     5041 gtcgagatac ggaataaaaa taaagtaaca gctgatttat ttttgctaga agtacagaaa
     5101 tatcatttaa atgaaacagt ctatgatgat aagacaggaa ggatcgatcc aagaaaattg
     5161 aatgctgtaa gccgtttggc aggaaatgat tatgcagcaa taggagaaat tttcacgatt
     5221 gcacgtcccg aataaaaagg agcactagtt atgttaaaaa taagctataa accatcgaca
     5281 gattcaaaag aaatgaaaaa agaatatgaa acagtgaatg attttctgca agggcagtac
     5341 ttagaagttc cgccattgca agaccatttt gtcgttacta ctgttacttt agatggtaag
     5401 gaaatagaaa tgccagatca aacgattagc ggattgttta attac
//
LOCUS       DACDMM010000155         5242 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDMM010000155 DACDMM010000000
VERSION     DACDMM010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.156"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            317..2152
                     /gene="tdc"
                     /locus_tag="IWD16_002336"
     CDS             317..2152
                     /gene="tdc"
                     /locus_tag="IWD16_002336"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9089334.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2457..3935
                     /locus_tag="IWD16_002337"
     CDS             2457..3935
                     /locus_tag="IWD16_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9089335.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3901..5232
                     /locus_tag="IWD16_002338"
     CDS             3901..5232
                     /locus_tag="IWD16_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9089336.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
BASE COUNT         1567 a          851 c         1114 g         1710 t
ORIGIN      
        1 taaaaaataa attgtgaatt tttaacaata taaaaaaata aaaatatgtt tagtgaatga
       61 aggacagtca ttttatttgt ttgttcgctt tttttaggtt atctatttta gaagactgat
      121 ttattgaaaa attgttcgct tttttgcgtt gaattatcag aaaaatccga ctttatttat
      181 ttttgtttgt aaaaatctac gtggatttga tgttataaaa aaaaatttct catttttggc
      241 gctatagtga agaggtgtta cagataaaca catgtatctg caagacattg accaacaaaa
      301 tatggaggga agaaaaatga aagatatgga tatcaaggcc gtctttatag gcgacaaagc
      361 tgaaaatgga ccagtttata aaatgttgtt gaacaagatg gtagatgaac atcttggatg
      421 gagagaaaat tatattcctt cagatatgcc tgcaattagc gaaggagata agctcactcc
      481 agattatcta gccacccgcg accatatgat agaggtcttg gatgaagtca gtcagcgatt
      541 acgcgcagga tcgatcccat ggcattcagc aggccgttac tggggacaaa tgaatgctga
      601 aacattgatg cctgctttat tagcttacaa ctatgcgatg ctgtggaacc caaataatgt
      661 tgcattggaa tcttctatgg caacttcgca aatggaagca gaagtaggac aggattttgc
      721 ttcattattc aatatgacag acggatgggg acacattgca gcagatggtt ctattgctaa
      781 tcttgaagga ttatggtacg cccgttgcat caaatcgatt cctttagctg tcaaggaagt
      841 gctgcctgaa aaagtgaaaa aaatgtctga gtgggaactt ttgaatttat ctgttgaaga
      901 aattttggaa atgaccgaaa gctttacgga cgaagaaatg gatgaggtca aagcagcttc
      961 ttcccgtagc ggaaaaaata tccaaagatt agggaaatgg ctggtccctc aaacaaaaca
     1021 ctattcttgg atgaaggcgt tagatatttg cggagtcggc ttagatcaga tggttgctat
     1081 cccagttcaa gaggattatc gaatggatat taatgctttg gaaaaaacaa ttcgtgaatt
     1141 agctggacaa aaaatcccaa ttctaggtgt ggttgctgtc gtaggaacga cagaagaagg
     1201 tcaagtagac agtgtagata aaatcgtcca attaagagaa aggttaaaag atgaagggat
     1261 ctatttctat ttacatgttg atgccgcata tggcggctat gcgcgttcac tgtttctaaa
     1321 cgaagcagga gaatttgtgc cgtatgcttc cttagctgaa ttctttgaag aacatcatgt
     1381 tttccaccac tgcgtaacga ttgacaaaga ggtatatgaa gggttcagag ctatttcaga
     1441 agcggattct gtcacgatag atcctcataa gatgggctat gtcccttatg ctgctggagg
     1501 aatcgtaatc aaacataaaa atatgagaaa tatcatttct tatttcgcgc catatgtgtt
     1561 tgaaaaatca gtaaaggctc cagatatgtt aggagcttat attttggaag gatcaaaagc
     1621 tggagcaacc gccgccgcag tctggacagc acatcgtgta ttgccattga atgtgaccgg
     1681 atatggtcaa ttgattgggg cttcaatcga ggcagctcaa agattcagag aatttctcga
     1741 tcatctgact tttactgtta aaggaaaaac aatcgaagtc tatccattga atcatcctga
     1801 ttttaatatg gtcaattggg tattcaaaga acaaggctgt acagatttga acgctatcaa
     1861 tgaattaaat gaaaaaatgt tcgatcggtc ttcttatatg gatggcgatg tttatggtga
     1921 acggtttatt acttctcata ctacatttac acaagaagat tacggtgatt ctccgattcg
     1981 ttttgttgaa agaatgggtc taacgaaaga agaatggaaa aaagaacaga aaatcactct
     2041 gctacgtgcg gctatcatga ctccttattt gaatgatgat cgaattttca atttttatac
     2101 aaaaaaaatc gccaaagcaa tggaaaagaa actaaacgaa atcatccaat agaactgatt
     2161 tttgtgtgaa agaataacaa gagacatatg gagaacagac aattatttgc ttcatcaaaa
     2221 caattgtatt tccccctgtt tttcatatat tcctcgccaa gatattcttt tttataaaac
     2281 gttctataag ttggatcgtt cgtttcatgt atcaaaccca gttcactaag atcgttcaat
     2341 ggatgtttcg gtgatactgg gttttgtact gaatcgatga aaccttgtca tttctgttct
     2401 tgaacaaaaa taaaagaaaa caaaaacata tgtcgctatt gaaagaagga ataaagatga
     2461 aagatactgg ttcatcaaag caaatcacga ccatgacctt tatcggaatg acctgcgccc
     2521 ttgtagcaag tgtacgaaat attccagatg tagcagcaac gagctggacg atgatttttt
     2581 acatgctggt tgcagtatta ttgtacgctt ttccaatctc attgatctct ggtgaatttg
     2641 ctggaatgtt tccccaaaaa ggtggtccag aactttgggt atcgaatgct ctaggaaaaa
     2701 aatgggggtt cgttgtctca tggctgttat gggtccaaat gtttcctggg atggtgatgg
     2761 tcgcttcagc actcgctccg ttgtttggta atattatcga taatgtgccg cttggtttga
     2821 acagcaaatt tacattagtt gtcattttag tcgtttattg gatcatcact cttttgaatt
     2881 tgaaatttga tatggcaaaa atcggcggaa aagtcggtgt ttggttagga ttgtacgtgc
     2941 ctttgacgat gatgctctta ttagggtttg ctgcgtggat caaagttggg atcgtaccaa
     3001 ccggaacatt aggcagtttt tcatgggaca aattaatacc agatactacg actgcatcct
     3061 catttgttta ttttgcgccg atcatgttta tttttactgg tatcgaaatg tcctctgtgt
     3121 atatcactcg attggaaaat ccagttaaaa cgtatattcg cggagtattt gccgcgttaa
     3181 tttttatatt tttcgtcaat attttgaatg ctttagttgt agcaaatgtc gtaccaaaag
     3241 gacaaatgga attgaataat atcgcgcaat ctatttcgat ttattgccaa attcttggtt
     3301 tgccacatct tattgtgaat ctgtttagtt tacttgtctt cattggtgta gcagtccaat
     3361 tatctgcttg ggcctcagga ccagcaaaga cagtgacaga aagtgctaga aaaggtgcgt
     3421 atccaccaaa attcaatttt tggaagacga atcaatttga cgtatccaaa tccgttattt
     3481 tgactcagtc tgtcattatc tcaatctttg cactttttta tttgctgatt cctggcgtca
     3541 atcaagcatt tttgatgctc gtaaattcaa ctactgttat ctactgtatc gtctatgtca
     3601 tcatggggat cgcagttttg agattgcgtt atacacatgc aaagttgaat cgaccattta
     3661 gaattggaaa aaaagagaaa aagagcaatc ttggagtttg gactgtcgtt attgttttat
     3721 ttgcggcaat cggattttct gtcggactga cgatgaaagc tggaacatgg atcaatctaa
     3781 ttgcagtcac agccatcagt gtcattttgt ttgtagtacc actctgtatt gaaaaaataa
     3841 agaaaccttc ttgggaacag gaagtcgcca tgctgcttga aaaagaaaaa ggtgaacttg
     3901 atggaaaaac cggcaatgaa acggactcta actaatcgaa atatccagtt aatcgcatta
     3961 ggtggcgcaa ttggaacagg attattttta ggttcagcaa aggcaattgc atcagcaggg
     4021 ccagctgtta ttctcgttta tttgatatgc ggtttggcta tttatggttt gatgcgggcc
     4081 atgggtgatt tatttttagc aaatagtaca tttaactcta tcagtgattt cgttgactat
     4141 tatttaggca aaaagtgggc attttttgtc aattggactt attggctttg ctggcttgga
     4201 gctggacttg cggaattaac agcaatagga ttgtatatcc atttctggtt tgtacatgta
     4261 ccagctttga taagcggagg tagcgcacta atgatcttaa tggcagtcaa ccttgttgca
     4321 gtcaagtggt ttggtgaatt ggaatctttt ttctcaatca tcaagatctt ggggattttc
     4381 ctttttatct tattgggtat aggattgagt gtctcagatt ttcataccca gtctatcgga
     4441 atcaaagaaa attttggaca gatcgatgaa ttactaccgt ttggctttgc tggaatggta
     4501 gtctcatttc ctatggtttt attcagtttt gctggaattg aaatgatcgg cttaaccgtt
     4561 ggcgagacaa gtgatccgca aaagaatttg aaaaaagcaa tcaactgtct gccttggcgt
     4621 attctctttt tctatatagg aacgattttg tcgattttat ttgttgttcc atggcattgt
     4681 ttagatttaa aagaaagtcc tttagtgact atgctgcaag tgatccattg ccaaggcgga
     4741 gcagcaattg tcaatctaat gattttggtt gcttctttgt cctcagcaaa tagtgcagtc
     4801 tacagtacta gcaggatttt atatcaaaca gcaaaagaaa aaagtttgcc ccattggttt
     4861 tgtgcgcttt ctaagaaagg cgtaccttta cacggtattt taattacagg aatgttattt
     4921 tttgccggtc tttgtctaca tttttttgta gctgatagtc gattgttttt tcagcttttg
     4981 gcaggcatga caacgagttg ttttctattt gtgtggtctt cgatcttgtg tgcacatgtt
     5041 caatttatta aagaaaagaa acggaacact acacaaagat ataaagatct tgctttgttg
     5101 gtgtttttta cttcaattgc tttcagcttg ttattcgaaa gcacgacacg ctttattcct
     5161 tttttattgg ttatctggtt tgtattctta aattttgtat ataaaagaat aagcaataga
     5221 aatatcagtt aggcccagat ac
//
LOCUS       DACDMM010000156         5235 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDMM010000156 DACDMM010000000
VERSION     DACDMM010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.157"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..997
                     /locus_tag="IWD16_002339"
     CDS             <1..997
                     /locus_tag="IWD16_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9089337.1"
                     /translation="KTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFYMQQE
                     LSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNVMGTQ
                     NVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIFSCVR
                     FGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSKFGET
                     YVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEIERSY
                     ENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVTW"
     gene            1009..2013
                     /gene="neuB"
                     /locus_tag="IWD16_002340"
     CDS             1009..2013
                     /gene="neuB"
                     /locus_tag="IWD16_002340"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP9089338.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2010..3158
                     /gene="neuC"
                     /locus_tag="IWD16_002341"
     CDS             2010..3158
                     /gene="neuC"
                     /locus_tag="IWD16_002341"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP9089339.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3131..3808
                     /locus_tag="IWD16_002342"
     CDS             3131..3808
                     /locus_tag="IWD16_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9089340.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3798..4940
                     /locus_tag="IWD16_002343"
     CDS             3798..4940
                     /locus_tag="IWD16_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP9089341.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            4953..>5235
                     /locus_tag="IWD16_002344"
     CDS             4953..>5235
                     /locus_tag="IWD16_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9089342.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINL"
BASE COUNT         1940 a          680 c         1025 g         1590 t
ORIGIN      
        1 aaaaacaata ttagtaactg gcggaactgg ttccattgga agtgaaattg ttcgtcagct
       61 tttaaaatat gaaccaaaag ctatcagaat ttatagtaga ggtgaagaaa aacaatttta
      121 tatgcaacaa gagctctcaa atcacactaa tttgagattt ttaattggtg atatcagaga
      181 ctctcaaagg attgattatg catgtcgtga tgtggatatt atttttcatg ctgccgcaat
      241 gaaacacgtt ccagcaagtg aatataatcc tatggaagct gtaaaaacaa atgtgatggg
      301 aactcaaaat gtaatagatg cagcgatcaa aaataatgta aaacattttg taggtatcag
      361 tacagataaa gtagtgagtc ctactaacac catgggtgcg acaaagttat tgagtgaaaa
      421 attaataata gctgccaatc agtataaagg aaaaacagaa acaatatttt cttgtgtaag
      481 gtttggaaat gtcatggggt caagtggatc tgttattcct ctttttagta aacaaataaa
      541 aaacaataaa gatattactt tgacagatgt tgatatgaca cgatttatga tgtcaatacc
      601 ggatgctgca agattagttc tgtctgctgc tatgaattca aaatttggag aaacatatgt
      661 cttaaaaatg ccggcaatca atattttaac tttagctcaa tcattaataa aaatatataa
      721 tgatacacat gatgaaaaat atagaggtga aataaaagtt gtaggtatca ggccaggaga
      781 aaaattatat gaagagctaa tgactacaga cgaaattgaa cgctcatatg aaaatgataa
      841 aatgtatatc attccaccac actatcatgc tactgacgaa ctgtacaaag attatgaaaa
      901 aattaaaatc actgcatact cttcagatgc aacagaatta atgagtggac aggaatttat
      961 tagaatcgca aaagagaata atgtcgtgac ttggtaagga gttttatgat gaaatcagaa
     1021 gtatatgtaa ttgcagaaat tggtgtaaac cataatggaa gtctagattt agctttagaa
     1081 agtattgata aagctaaaga atgtggagct aacgcggtta aatttcaaac atttaaaacg
     1141 aataagctag tttccaaaaa ggctccaatg gcaaaatatc agaaggataa tcttaaagag
     1201 gacagttctc aatttgaaat gctaaaaaaa ttagaattat ctcaatctga ttttattaga
     1261 ataaaagatt attgtataca aaataaaatt gattttttgt ctactccttt tgatgaagaa
     1321 agtgcagact ttctaaaaca aattggtgta caaggattta aaattggctc aggagattta
     1381 acaaatttac cttttcttag aaaaatagat atgtatgggt tgccagtttt attatctaca
     1441 ggtatggctg atattcatga ggtcagtgag gctgtagatt gtttcataga tagtccagtt
     1501 actattttac attgtacttc aaattatccg gctagttctg aagatattaa tttattggcg
     1561 attaacacat taaaagaaat gttcgggata cccgttgggt actccgatca ttcattggga
     1621 tatgatgttg gtatttgtgc tgttgcgatg ggtgctaagg taattgagaa acattttacc
     1681 ttggataagg aactacctgg tccagatcat aaagcttctt tagatcctca ggaatttgca
     1741 gattttgtaa agcatattag aaacactgaa atatttttag gtgatggtgt taagcgagca
     1801 atgccttctg aacttagtac aaaagaagta tcaagaaaaa gcctagtggt cactgagaat
     1861 ttaaatgtgg gagatattct tacagagaaa tctcttacaa taaaacgtcc tggaaatgga
     1921 atagctccta agtttttata tcaatatatt ggcaaaaaag ttaaaagacc aataagtgca
     1981 gatagtgttc tgcttgagga agatgttgaa tgaaaaaaat acttatagta agtggaacac
     2041 gagctgatta tggaatatat aaacctgttg ctgagaattt actacaagat aattgggatg
     2101 tatcatttgt tgtttctgga atgcatttat cccataaata tggatttact aaagaattga
     2161 tcttagctga cggatttaga attagtggag aagttcaaag tttgttttta gagaacaata
     2221 agggaaacat ggctcggtct gtggcattag agataaatgg tttcacaaat atatttgaac
     2281 aaactagccc tgattttgta ttattactag gggatagagg agaaatgttg gcagctgcga
     2341 tagcttgttt gtatcttgga atcaaaacag cacatattca cggaggcgaa gtgtctggta
     2401 ctgtagatga atcaataaga catgccataa cgaaaatagc atctgttcat tttgctgcta
     2461 cggaaaaaag caaagagcgt ttaataaaaa tgggggaaga ttcttggaga gtttttcccg
     2521 tcggagctcc tagaatagat actattttaa ataatagatt acctagtttt agagatataa
     2581 aagaaaaata tcatctaaaa tttgaaaaga aaaaatacgt tttgcaagta tttcatccag
     2641 ttgtaaccga actagaaaat atagaaaaac aaattttagc tttgatcaat tcaatggaac
     2701 aacaatctca accaattatt tgcattttac ctaatagtga tgctggtagc gaaattatca
     2761 gaaaaatgta ccagaaatat ttaaaaacgg atattattta tattacgaat ttagagccgg
     2821 aggaatacct gactatatta aaagagtgca aatttatgat cggaaattct tcttctggga
     2881 taattgaagc tgcatcattc aaaataccag taataaattt aggaaccaga caaaatggac
     2941 gagaacaatc taaaaatatt ataaatgcaa atctaaatga aaatgaaatt gaaactgctt
     3001 tgaataaaat acaagatcct cagtttataa aaagcttgaa aaatgttaag aacatctacg
     3061 gtgacggcaa cagttctaga agaataacaa aaacattgaa tcaaatattg aatgatgggg
     3121 aagatattaa gtggattcaa aaacaaatag cttattaaaa tatactagtg atccatacat
     3181 tattattggg gctggcggac acgcaaaagt cattattgaa accttaagat tagaaaataa
     3241 gttaatatat ggattttttg atgataagca aaataaaatg caagatctga aatatcttgg
     3301 gaaaataaac gaattgaata gctgggcgga tagcaaaaaa aaaatctata aatttattgt
     3361 agctattggt gactgtgtag taagaaaaaa gattataaaa aaattgaata tgccaagtga
     3421 gcaatatgga atagctgttc atccaagtgc tatagtggca aaagaaattt caattggtta
     3481 tggaactgtt atttttgcga atgctgtaat taacccagat gctgtaattg gagagcatgc
     3541 tataataaac acaggtagca ttgttgagca tgattgtaga attaataact atgttcattt
     3601 atctccaggt gtatgcttag cgggtggagt acatgtaggt gtaggaactc aaataggaat
     3661 tggttctcaa tgtatccaga tgaagaaaat tggatcttgg tgtatgatag gcgcagggag
     3721 cactattgtt aaggatattc cctcacattc cttagcttat ggcaatccag ctaaaattaa
     3781 gaaagaagga attaattttg aattctagaa ttttattatc aagtcctcat atgggaggaa
     3841 cagaagaaaa atatgttgct aaggctttta aagataattg gatagcgcca ttaggtgaaa
     3901 atgtggataa atttgaagaa agtattacaa aatatactgg ttctaagggc ggagcttctg
     3961 cgttaagttc aggtacagct gccatacatc ttgccttaag actgcttaat gttggaaaaa
     4021 acgatattgt ctttgctcct tcatttactt ttatagctac agtaaatcct attttatatc
     4081 aaggggctgt accagtgttc attgactcag aacctaatac atggaatatg agccctgttg
     4141 cactagagag cgcattagaa gaagctgaaa gaaacaacca attacctaag gcaatcatcg
     4201 tagtgaattt atatggtcaa atggctgatt atgaacgaat tttagctatt gcaaaccaat
     4261 ttaatgttcc agtgatagaa gatgcagcgg aatcgttagg ttctacttat aagggaattt
     4321 cgtcaggctt atttgggaca attgggatct attcatttaa cggtaataag atcatcacaa
     4381 cctcaggtgg aggagctcta gtgtctagtg ataaaaaata tgttgagcat tcaagatttt
     4441 tagcaacaca agcaagagat caagcaccgt attatcaaca tagcgaaata ggttataatt
     4501 accgtctaag caatatttct gccggtatcg gacgtggaca aatggaagta ttgaatgata
     4561 gagtagctaa acgaagagaa attttcaatc gatataagga agctttttct ggaattgata
     4621 gaattgaaat gatgccagaa gtggatggca tggtgccaaa tcgttggttg tctactttaa
     4681 cgatcaatga ttataattct attgatcaag tagttaacgt aataaataaa ctaaatgaaa
     4741 aaaacattga aacaagagct ttgtggaaac cactacattt gcaaccagta tttcaagggg
     4801 taaaaatgta ttaccatgat aataatctta gtgtgtcaga aaagttattt aatagcggct
     4861 tatgcttacc ttctggaagt aatatgacag atagtgaaca agaatttgtt attgatgagt
     4921 ttaaaaaaat attcaagtag gtgtgatatt ttatgaacat aggaattatt ggtttaggtt
     4981 ccatggggaa aagaagactt agactcattc aagaacactt taatgatatt ttactatgtg
     5041 gtgttgatac taatgaggcg agacgaaagg aagttgaaaa ggaatttgga ataccaactt
     5101 ataatagtct aacagaggca aaaaatttgt ttgaaataaa ttcagttttt gtttgtacat
     5161 cccctataac ccatgaattt attataaaag aggctttaaa tttagattgt aatatcttta
     5221 cggaaatcaa tttat
//
LOCUS       DACDMM010000157         5223 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDMM010000157 DACDMM010000000
VERSION     DACDMM010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.158"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..136
                     /locus_tag="IWD16_002345"
     CDS             <1..136
                     /locus_tag="IWD16_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9089343.1"
                     /translation="QGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL
                     "
     gene            complement(214..747)
                     /locus_tag="IWD16_002346"
     CDS             complement(214..747)
                     /locus_tag="IWD16_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP9089344.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(841..2019)
                     /gene="mutY"
                     /locus_tag="IWD16_002347"
     CDS             complement(841..2019)
                     /gene="mutY"
                     /locus_tag="IWD16_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP9089345.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2012..2809)
                     /gene="recX"
                     /locus_tag="IWD16_002348"
     CDS             complement(2012..2809)
                     /gene="recX"
                     /locus_tag="IWD16_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP9089346.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2916..4298
                     /gene="rlmD"
                     /locus_tag="IWD16_002349"
     CDS             2916..4298
                     /gene="rlmD"
                     /locus_tag="IWD16_002349"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9089347.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4472..5050)
                     /locus_tag="IWD16_002350"
     CDS             complement(4472..5050)
                     /locus_tag="IWD16_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9089348.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         1524 a         1058 c          937 g         1704 t
ORIGIN      
        1 tcaaggctat aatacgattt tattaggggc acagtccaca gctgaaactt tttacgaaaa
       61 actaggatat acagcttacg gcgatccttt tgaagatgct ggcatgcctc atatttatat
      121 gaaaaagact ttataaaaat ttatttcgaa aaaagcaaaa aacagcaagc ttttgagaaa
      181 aatctctcac tggcttactg ttcttttttg tgtttactta cgcgacaatt gcttgtaacg
      241 gttataccaa atatcaatat agccttccga gaacgggcca tctccattat tgatccagtc
      301 aaccagaatc ttcacatttt cttttaggat gaagtcaata tcgttcgggt aattcatcat
      361 tttgctgtgc atctcatatt catcgacatc caatagtcgt ttctcgccgt caggaaatac
      421 cttgatatcc aagtcatagt cgatatattt caaggcctca tcgtccagca aaaaaggtga
      481 agctagatta caataatagg aaaccccctt ttctctgatc attgctatta cattgaacca
      541 gtatttttta tgaaaataaa caatcgctgg ttcgcgcgtt acccatcggc gtccatcaga
      601 ttcagtgact aatgtgtgat cattcacgcc gatcatagag tgttcgcttg tttttaatac
      661 catggtatct cgccacgttc gatgcaattt gccgtcgtgc ttgtaacttt ggatcgtcac
      721 aaactctcct tcttttggaa ctcccatagc aacccttcct taagcagcat tttgtgcttt
      781 gtgtttgggt gttcattaca ccgacatcat aattattata tcaataaaaa tgagctttgc
      841 ctagaaagga ttcctattgt ttcgattttt ctctaactga tctaccagtt tcatttggac
      901 cttaggaaaa acgaccgaat caaatgcatc aggcttcaac cacttcgaag ttttgttctc
      961 ggtaaattct tgttctgctc cctcggttgc ccgtccgtaa aaaaggagaa catgccattt
     1021 cagatgacta aaaatatgag taacttctcc taaatgtcgt gtctgccaaa caaagagttc
     1081 ctgcttttca aatgggagat tttgctcttc ttctgctact aggtcatcaa acagatcgat
     1141 ttctggcttt gtttcccatt cttttttcaa tcgctcatac tcctcttgtg tcacttccat
     1201 cattggaaat gtccacatat tcgccagcaa tttttggcta tctctttctt caaaataata
     1261 cgctccagaa tgattttgaa gagctgcact gatataataa acgtcttttg gctttgcttt
     1321 ttttgttttt acaggaaaac tcgtttggat tcctcgtttg ttagctaaac aaaaggcttg
     1381 gatcggacaa gtctcacact ttggagaagt tggtgtacag atagctgaac ctagatccat
     1441 cattgcttgg ttgaattcac ctggatgctt ttcatcaatg attttccgca tcgcttcatc
     1501 aaagattttc ctgctagaag cttttgcgat gtctgcttca atgcaaaaca atctgctgac
     1561 tactcgcatc acgttgccgt caactgcagg ttctggaagt ccaaatgcga tacttgcaat
     1621 cgcgcctgtc gtatatggtc ctattccctt caatgagcta atttcttcag gcgtttgcgg
     1681 catttcccca tcaaattctg acatgatctg tttagcagca gcttggatgt tccgggctct
     1741 tgagtaataa ccaaggccct cccatgcttt taaaagcttt tcttctggag catttgctag
     1801 ctcttcaatt gtaggaaacc attccatgaa acgataaaaa taatcgatca ctgtgtctac
     1861 tctggtttgc tgaagcatga tctcagagat ccagatacga taaggatcac gattatagcg
     1921 ccaaggcagg tttctttttt cttgttcata ccattgaatg aactgatctt gaaattcttt
     1981 tgtttcttta tctgtccatt tttcccattc tttattcact ttcctcttcc ttttccgtaa
     2041 tgaatcgctg gatcatatcc aaatcaaatc ctttttgata taagctttgc tttactttta
     2101 gatttctttt tttaggttca aaccttaaat ttcttcgcca tagtttatct ccttgtttga
     2161 ctaaataatc atattctatt tcttgatctg cttcttgcgg aagatcagct atcgcttgtt
     2221 ggatcacttc ttgagaaaag cctttcgtca tcaatgtttg ttggattttt ctcaataatt
     2281 ctttttggct ttttccgtga ttttttccaa acgctttttc ggctgtctga tgtgcatttt
     2341 gaacttggtc ttcaaatgta tattggctga gcgcttgttc aatgatttca ggtttcaaac
     2401 ctttttgctg catctgctgc gctaatttct ttggtccttt gtcacttaag cgcatattcg
     2461 tgcgcacata actttcccca tagaccaaat catcgacaag gtccaattct tttaagcggg
     2521 ctacgatttt atttcgatct tcttgaagga tttccttttc ttttaagtat gttcggattt
     2581 ctttttccga tcgcaactga taacttaagt aattcattgc taggtgaaaa ccgaaatcag
     2641 ctccgctact cttcttgatc tcttggatag tttcttcgtc taattcccgt ccttttaata
     2701 atcgatagcg tactaataca tcttcagata cttgtaaagc ttctccttca gataaatcta
     2761 cgcgatagaa agggcctttg cctttactga ttctcgtaac ggttaacata aacatcctcc
     2821 tcgaacatat attctctatc ataccacaga aaatccccgt aatcaggtat tatatgttag
     2881 aataaaccac agaaaaaaga aaaagggaga tacttatggc agaaacaatt gtcaaaactg
     2941 gacaaacggt tcctttgaaa atcaagcgtt taggcataaa tggtgaagga atcggttatt
     3001 tcaaacggtt gatcatattt gttccatatg cactaccaaa agaagaagta ctagtgaaaa
     3061 tcacgaaagc aactcctcgc tatgcagaag gagaattaat aaaagtcaaa aaaacaagca
     3121 aagatcgcgt agtggcacca tgtcctgttt actatgaatg cggtggctgt caattacaac
     3181 acttagctta tcaagctcaa ttggatttta aaaaggatct gcttttgcaa gcattggaaa
     3241 aattcaaacc agcaggcttt cgatcttatc aacttttgcc gacgatcggg atggatgagc
     3301 cttggcatta tagaaacaaa gcacaatttc agttacgaaa aaataagcag actcaaaaaa
     3361 ttgaagctgg tttatacgaa gcaaactctc atcagttagt ccctataaca gactgcttgg
     3421 ttcaagaacc tcgaacacaa aaagtaatga atactgttgt tcaactattg aataaatttg
     3481 atgcacccat ctatgatgaa cggacaaaca gtggaatctt ccgcacaatc atggttagaa
     3541 tcggtgttaa aactggggag ttacaagttg tttttattac tcgcagtaaa aaattcccgc
     3601 aaaaaaacgc aatgatccgt gagatcaatg aacgactgcc cgaagttgtt tcaatcatgc
     3661 aaaatgtcca ggcacaaaaa acgtctctcg tgatggggga tgaaacgatc cacttatggg
     3721 gaaaagaagc cattgaagaa caaatcaatg aagtgacttt cgatctttct cctcgtgcct
     3781 tctttcaact caatcctcag cagaccgaat tgctttatgg cgaaggaatc aaagcattag
     3841 atgtccagcc taatgaaacg atcgtggatg cttattgcgg agttgggacg atcggcttaa
     3901 gtgtagcaaa acaagccaaa gaagtacggg gaatggatac cgttcctcaa gcaatcgctg
     3961 atgcaaaaaa aaatgcggaa agattaggat atcacaacac tcgatatgaa gtcgggacag
     4021 cagagcaagt tctgcctaaa tggttgaatg aaggttttca gcctgatggt atcatcgtag
     4081 atccaccacg tacgggtttg gataacgcat tgatccaatc attattgaaa taccctccaa
     4141 aaaaacttgt ttatatctcc tgtaatgttt ctacacttgc cagagatctg gttaatttaa
     4201 gtaaaaagtt caagattgaa tatttacagt ccgtcgacat gtttcctcag actgcacgtt
     4261 gtgaagttgt cgtgaagatg acgagactcg ctgactaaat gaaagaggtt gtgaaaaagt
     4321 ctgccctgta tcaagtaata agaacagcca aacaaaaata gctcttcatg ttttatgaat
     4381 actgtacttg tgaccgaaga gttaacagga ggacactatt tatttaaaaa aagggttgtg
     4441 acatagtttt tgtcacagcc ctttttgatt attattggat attcacgtct atcacttgat
     4501 aaaatgcatt tgtcgtatcg ctgatattcc atactgctaa aatcacatta tatccttttc
     4561 gatcctgcgg aagtgtgatc gtttggttga ctgtttttgg tggaacagtt gctttgtcgt
     4621 ctatttttgt gatcaattca aaatcactaa atttcaaagg ttgattagga ttccaagttg
     4681 gtttggtaat gtagtaatcc caagtacttg tccgatgttg tgctgttaaa ttccaagtaa
     4741 tatttaatgt tcctggatta accatgtttt tatgccatct gttagatgtc tgttcatcta
     4801 aaggctcaaa gccgctgact cctgcactag caatttttcc atcaatgaat gtatttttgg
     4861 tagcttcgat actttgcggt tcccattgtg ctcttccgac attttgattt aagtttcctg
     4921 ctgctgatgt tcctaggtat gcacggcttc ctggagaagt aatataacca tgcgcaaaac
     4981 ctggaatgct aaaaccgaac aaccctaaac taactaaccc aataaacaat aacgtactaa
     5041 tctttttcat gtagaaacct ccttcatttg ataaccacat tgtaaacgct ttattgtatt
     5101 aacacaaaca ttatgctttt ataattttat gttgaagtaa aaaaggtgaa aaaagtaaga
     5161 tgcagattat aacagaagta gacgagaaaa gtacaaatag aaaacatgag aatcaaatca
     5221 tat
//
LOCUS       DACDMM010000158         5119 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDMM010000158 DACDMM010000000
VERSION     DACDMM010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.160"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(232..1380)
                     /gene="nagA"
                     /locus_tag="IWD16_002351"
     CDS             complement(232..1380)
                     /gene="nagA"
                     /locus_tag="IWD16_002351"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP9089349.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1456..2292)
                     /locus_tag="IWD16_002352"
     CDS             complement(1456..2292)
                     /locus_tag="IWD16_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9089350.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2476..3693)
                     /locus_tag="IWD16_002353"
     CDS             complement(2476..3693)
                     /locus_tag="IWD16_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9089351.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            4076..4159
                     /locus_tag="IWD16_002354"
     tRNA            4076..4159
                     /locus_tag="IWD16_002354"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4110..4112,aa:Leu,seq:gag)
     gene            complement(4399..>5119)
                     /locus_tag="IWD16_002355"
     CDS             complement(4399..>5119)
                     /locus_tag="IWD16_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089352.1"
                     /translation="IFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPF
                     FLDMNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGN
                     KRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSI
                     FLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNE
                     FMQFGLTVLGICTFIITVINR"
BASE COUNT         1579 a         1044 c          810 g         1686 t
ORIGIN      
        1 aaataagttc atctttctcc tcctaattat gttcatttat tcacaataaa aaaagctttt
       61 attttgctaa tacgtattaa catttttaat tttatcttta cattgttact tattgaacaa
      121 taggaaatta aagaaacttt tgaaacttat gtaaagtgat ttttactaga acacaaaaaa
      181 accttaaaac caaacttaag aagtttagtt ttaaggttaa cttaaattat attatgcgtg
      241 gaaacgttcc acgccatcta gataagttgc ttccaatgtc atatcagggt tcaatacgat
      301 aaagtctgca tcgcgtcctt gagcgatttt tccacatgta tcatcgattt tacagctgat
      361 tgctggaatc agactagcca tcatgactgc ttgttctggt gttgctaatc cccagtctac
      421 aacattttta attgcttctt tcaatttcaa tatacttcca gctaagttgc cagattctag
      481 acgtgctgtt ccttctttta ctactactgg aaattcgcct aaattgtaat ttccatcagg
      541 catcccaccg gccatcatac agtctgtaat taatgcgaca tgatcatgtc ctgctttttc
      601 cattaggatt cccgcagcat ttggatgtac atgatggcca tcacaaatca attcagaaaa
      661 gacatgttct aaagataata acgcacccac catacctggt tcgcgatgat tcaatccacg
      721 cataccattg aaagcatgga caaatacact tgcgcctgct tcaacagctt tttgcgcttc
      781 ttctagtgta gcattactgt gtcctagaga aacaactact ccttcttcag tcacttgttt
      841 gacaaactct tctacacctt gacgttccgg tgctaaagca atttttttga ttaaaccacc
      901 tgaagcttct tgccattcat ggaaagtatc caaatcagga tccccgaaat aacttgggtt
      961 ttgtgcccct ttgtgttctt ctgtgaagaa aggaccttca aaatagattc ctttgatttt
     1021 agctcctggt gcttcctgat acattttgcc gattgtctca gctacatctt ttaaacgttc
     1081 tttgcttgac gtcaatgttg ttggtaaaaa tgaagtcact ccacaagata acagaccttc
     1141 agacatcact ttgatgcctt ctgcatcatt atccattaca tcatgattca tatagccgtg
     1201 aatatgtgta tctaccaaac caggagcaat ccatttgccg ctttggtcaa tgatgttgac
     1261 ttctccctca ggtagttctt tggtgtattc accaaaaagt ccatcggtaa tttcaagata
     1321 gcctgcatgt ttgacacctg acgctaaaaa gaatttgtca gcatagataa aagttctcat
     1381 tctattctct ccctttgttt ttctacctct aaagtactct tatttagaga agaagtcaag
     1441 aatggtctac accatttaca aattttttga aatttgtttc acagcttgtg ctaagccttc
     1501 atagaatgct gctctttctg gttcttcttt acgcttgtat attaagcttt taagcacaaa
     1561 taaatcattg aggtaatagt agctattctt ctcttgagcc caatagattc cttgattcac
     1621 ctgttctaac gcttcgtcga tttcattttg atggaataag aaatttccat aattataaaa
     1681 aatttgcgaa aattcacttt ttgatcgttc atcagctgat tgatgcagca gttggatgct
     1741 tcgactcaaa tagtagcgtg cttcagccgt ttttcctgcc acaccatata tttttccgat
     1801 acaactaatc agttgtattt ctgtgtcaga aacatggatt ctttcttttt ggctagagta
     1861 gcttaatgct tctttcaaat aataaaggga ctgagagata ttttgacgca acgtgaattc
     1921 gcaaacaccg agataataat aatagcgttg aaagtcagtt gcttgataca gattttcaga
     1981 aatatcagaa gattttagta cttgttctaa cttccgataa tcacgttttt ggaaacaact
     2041 atccaacagt tcaaataatt tatcgtttgt ccgagtagtt tttcgagaga gattcatcac
     2101 atgatcaatc gttaccccta gccgttgaca aatctgcatc atcaccaata cattcggcag
     2161 ttcctcatta ttttcgatcc gacttaatac actttgtgaa caaatatcat gcgaaagcat
     2221 tttttgcgtc attttttgtt cttttcgaat ctctttaatg atcgttccaa aggactcgat
     2281 taacgtttcc ataagtatcc tccccaaaat atgcgtattc gcattctatt cgtattttac
     2341 tttaggaagt cgcaaacttc aagaaaagac ttgcaactaa ataatatact ttagttattt
     2401 aagaaaactc cactagaaag agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa
     2461 atttggtttt taaaatcatt tttggcgata ggttgctagg aattctctca ttttcttagc
     2521 tgtttgtttc aaatcatcca ttttaggaag ataaacgatt cgaaaatgat ctggctggtt
     2581 ccaattaaat ccaccaccat gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac
     2641 gaatttttca tcatccaaaa tattgaatcg ctttgtatca atttttggga atatataaaa
     2701 agcagctttt ggttttacgg ctgaaagacc aggaatatca ttgattgcat tatagatata
     2761 ttctctttgt tcatagatcc ttccgcctgg caggagcagc tcatcgacac tttgataacc
     2821 gcctagcgcg gtttggataa tttgctgaga caatacattg gaacacaaac gcatggagga
     2881 caacatattc agcccttcta tatagccttt gacgtgtgac ttatctccac ttaggaccat
     2941 ccagcctact cgaaatcctg ctacacgatg agatttggat agaccgttca gcgtaacaac
     3001 gaacaaatca ggcgctaatg tagcaatcgg aatatgttct agaccatcca tcactaagcg
     3061 atcatagatt tcgtcggaat aaataatcag gttattttgt cgagctactt caacgatttc
     3121 ttctaataat tcttttggat aaagtgcccc agtaggatta ttaggattaa tgataacaat
     3181 agcttttgtc cgactcgtta tttttgattt gatatcatca atatcaggat accattctgc
     3241 ctcttcatcg caaatataat gaacaggtgt gcctccagct aatgcaatag aagccgtcca
     3301 caacggatag tccggcattg gaaccagtac ttcatctcca ttgtccagca acccctgcat
     3361 acacatcgtg atcaattcgc tcactccgtt gccggtgtag atatcattga tcgtcacatt
     3421 cggaaaatgt ttcagttggc aatattgctc aattgctttt ctagcagaaa agatcccctt
     3481 ggaatctgag tagccttctg agtcacgggc attcatgatc aaatcacgaa tgacttcgtt
     3541 tggtgcttca aatccaaaag gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc
     3601 ctcttcttgc atccgctccg cttcttctag aacgggaccg cgaacatcgt aactgacacc
     3661 ttctaatttg ttggattttt caaattctct catcacttag ccaatccctc atttcaaaaa
     3721 gttagaactt tccaaatttt ttgttattta ctatttcaat atcatactcc atcctagaaa
     3781 attttgccac ttatttagga aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa
     3841 cttaattgtc atgatagtca ttccatagat tcgctgccca atccattgca attccacaaa
     3901 tgattcctag cataaaacca agcaaaaaac gtccgaatgc aagtccaatc aatacaccta
     3961 agatcattcc cagtcctgaa aattctccgt catatttctc catcttaata agtcctccca
     4021 tttttgagct tgacaactat gtaaaacctc gctatcatta gtgtgttatt attacgcaga
     4081 tgtgttggaa ttggcagaca agcatgattg agggtcatgt gagcgttagc tcgtgtgggt
     4141 tcgagtccca tcatctgcac tacaactcgt gaagttaatt ttgcttcacg agtttttttg
     4201 tttgaacgtt tctttatgcc attatataca tatcactgta tcctttacaa aaagaacgta
     4261 caatcatgta aacagcctga ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa
     4321 cttgcaagtg ttaaaacaat taatcgaaaa acgagtaaaa tccctcacct aatactaaag
     4381 cgagggattt tttgtatatt atctgtttat caccgttatt ataaaggtac aaataccaag
     4441 cacggttaat ccaaattgca taaattcatt tttttgtaca ctcatgcttt tcattagctc
     4501 tttaactgcc attagtacta agatggcgat aacaacataa agtatatagt acgtccgcat
     4561 tccggaagcc catactgtgg gagaaaatcc cataattaac cttgaacaaa atcccattat
     4621 catcaaaata actaaaaaaa tagaagtttt cttattgtca aaacttaaat aaattccaac
     4681 aattaaacat agtaataatg ccaagactac taaagtagct atccaagtac ctggataaaa
     4741 aagtgacagt ttcgttccta actctgtaaa gagattattc aggttactac tactccaaat
     4801 cattgccctt ttatttccat tgacgtaagt aaagctttga cccattgtat taccaaaata
     4861 aataatcagg tttaagaaaa acggtaatgc agtcaatata cgcacataat agttttgaca
     4921 tttcctatat gttagaagga aaattaagaa aaatagcaat aaaaataaga tattcatgtc
     4981 taaaaaaaac ggctttccaa atgatgaaat acctaaatct aacttattaa gaaagttgaa
     5041 atttttatat tcaggaaacc atttattaat ttcatgtgca gcacgtaatg cattccctgg
     5101 agttgtcaaa gagaaaatt
//
LOCUS       DACDMM010000159         5066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDMM010000159 DACDMM010000000
VERSION     DACDMM010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.161"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            49..1689
                     /locus_tag="IWD16_002356"
     CDS             49..1689
                     /locus_tag="IWD16_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP9089353.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            1843..3165
                     /locus_tag="IWD16_002357"
     CDS             1843..3165
                     /locus_tag="IWD16_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9089354.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3233..3667)
                     /locus_tag="IWD16_002358"
     CDS             complement(3233..3667)
                     /locus_tag="IWD16_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9089355.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(3732..4569)
                     /locus_tag="IWD16_002359"
                     /pseudo
     CDS             complement(3732..4569)
                     /locus_tag="IWD16_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
BASE COUNT         1548 a          987 c          984 g         1547 t
ORIGIN      
        1 atctgacttc tatacttcgt ttgtattaaa acttaggagg aatcattcat gcttacaggg
       61 ttaaacttat taaataatcc ttttctaaat aaaggaacag cttttacaaa agaagaacgg
      121 gcaaaatatg gaatcactgg tatgcttcca agtactgtcc aaacactgga acaacaatct
      181 gttcaagctt atggtcaata cttaagcaaa caaacagatt tggaaaaacg tatcttctta
      241 atgaatctat tcaatacgaa tcggacattg ttttataaat tgatgggcga acatttagtt
      301 gaattcatgc ctgtcgttta tgatcctgtc gttgcggatt caatcgaaca atacaacgaa
      361 attttcttga aaccgcaaga tgctgcattt ctttcaatcg acgaaccaga aaacatcaaa
      421 gcaagtttga agaacgcggc agatggacga gacattcgtt tgattgttgt gactgatgca
      481 gaaggaatct taggtatggg cgactggggt gtgaacggtg ttgacatcgc tatcggcaaa
      541 ttgatggtct atactgctgc tgctggtatt aatccagcac aagttttgcc tgtatcgatc
      601 gacgctggta ctaataatga agaattattg aataatccct tatacttagg caatcgtcat
      661 gctcgtgtag aaggcgaaac ttactatgaa ttcatcgatc aattcgttca atcagctacc
      721 gaattattcc cagaattgtt attgcattgg gaagattttg gacgaggaaa tgctgcagcg
      781 atcttagaaa aatacgaaga tcaaatcact actttcaacg acgatatcca aggtacagga
      841 attgttgtcc tagcaggtgt attaggcgga ttgaatatat ccggtgaaca attgaaagat
      901 caaataattt tgacatttgg tgctggtact gctggtgtgg gtatcgcaaa catcctttta
      961 gatgagatga tccgtcaagg tgtacctgaa gaagaagcac gccaacattt ctatcaagta
     1021 gataaacaag gtttattgtt tgaagataca gaaggattga ctcctggtca aatcccattc
     1081 gctcgcaaac gctcagattt cactaatgct gatgaattga caaacttaga agcagttgta
     1141 aaagcgatcc atccgacaat catgattggt acttctaccc aacctggagc attcactgaa
     1201 gaaatcgtga aagaaatggc tgctcataca ccacgtccga tcatcatgcc tttgtctaat
     1261 ccaacaaaat tggcagaagc aaaagcaaaa gatttaatag aatggacgga cggaaaagca
     1321 ttagttggaa caggtatccc tgctgctgat gttgaatata atggtgtaac ttaccaaatc
     1381 ggtcaagcaa ataatgcttt gatgtaccca ggtttaggac ttggtttgat tgcttcaaca
     1441 gctacacgag taaatgctga aatcatttct caagctagtc gagcattagg cggaatcgtt
     1501 gatgtaacaa aaccaggagc agctatcttg cctccagtag caaaaatcac tgaattttct
     1561 caaacgattg ctgaaactgt cgcaaaatca gttgtcgcac aaaacttgaa tcgtgaagaa
     1621 atcacggata tcaaagaagc agttgaatct gctaaatggg ttcctgaata taaatcttta
     1681 gaagattaag atcgttatag taaaagaaca tacagaaact ttgtatgacg tctaaaaaga
     1741 aaaaagcgac tgcggcaaaa cagcggccag ccaactcctg ctcttttgtc gcttttcttt
     1801 ggcttttttc atatgataca gaataagaaa ggatctgtaa aaatggcaca aaaaacaaca
     1861 actgcaactg cagttgagga aaagaaaaaa gaaggttttt ggtccacaaa aatcaccggc
     1921 gtaagtttgc cactttattt gatcatggtc cttgtcctga tcattgcaat ggctttaggg
     1981 aaattaccga cgaatatgat tggaccaatc gcagttttgg ttattttcgg taatttattt
     2041 cattttatcg ggacaaaaat ccctatcgtc aaaagttatt tgggtggtgg ttccgttttt
     2101 gcgatcttcg cttctgctgc gattgctaca tttgggatct tacctgaata tgtagtgaaa
     2161 tccacagaaa actttgtcag caatatgggc ttttgggact tttatattgc tgccttgatt
     2221 actggtagca tccttggtat gaaccgtaat ctattgatga aagcttctgt tcgttttatt
     2281 cccgttgcac ttatttcaat ggtcgtctcc ttttttgccg taggactcgt gggcatgctt
     2341 atcggaaatg gttttgcaaa ttccgtttta tacatctcat tacctgcgat ggctggtggt
     2401 gttggagccg gagccgttcc tttatcaacg atttatgcaa atgttttagg tacggatgct
     2461 tcttctatca tttcccgcct gattcctgcg acagcaatga caaatgtctt agctatcatc
     2521 ggtgcagcat tagttgctcg tgctggacaa tcaatgccaa aattcaacgg caatggtaaa
     2581 ttaatggaaa aaggcgatct tggcgatgga aaacctcgaa ctgtgaaacc ttccattcca
     2641 cagttaggtg ttggtttgat ggtatcattg actttcttca tcttaggaca aattggtaac
     2701 tattttgttc ctaaagtcca tgcttacgca tttatgatta ttatcgttgt catctgtaaa
     2761 atcgccaacc tattaccaga gtattatgaa gatgcagcta tcatgttcaa taacttgatt
     2821 gtcaaaaact tgacagcagc tgttcttgcc ggtatcggta ttgcgttgct gaatctgaat
     2881 gtattagctt ctgctttgac atggcaattt gttgtcttat gtctgacaag tgttatcgcg
     2941 atttcagttg tttcaggatt tgtcggtcgt ttgtttggtt tatatccaat tgaatcaact
     3001 attactgctg gcttatgcaa caactcgatg ggcggaacag gaaatgtagc cgtcttatct
     3061 gcagctaatc ggatggaact tattgctttt gctcaaatgg gcaatcgact tggtggtgcc
     3121 atcgttttga tcatctctgg tttcttgatg caattacttt cttagtcaaa aaaacaaaaa
     3181 tacctgatca tcaagtatcg gcttggtgat cgggtatttt tttgataaag atttacagga
     3241 ttttcaaaaa tcttttttgc attttatcgg aatgataacc gatgtgttca taaaattggt
     3301 gtgcttctac tctgtattct gcagaattaa gccttattgt cgttattcct cgttttttcg
     3361 cttcttcttc tacgccagtc atcaaagcct ttccgatgcc tcttttttca gcatatttag
     3421 acacagcaag tgccataata ttgaacattg gatcgctaaa aatcgattca taggactccg
     3481 cttgtacata gccagctgct tttttagaag taggatcttc ataaaccaag aaaaaatttt
     3541 tctctggctc cttcagcagt ttcttcattt gtctcaccgt aacggttaat ggtatatcat
     3601 accctaattg atccgcattg atttcacgta gtgcttctgc atcttctaaa cgcatttccc
     3661 gtaacatcca aatcaccagc caatttctga ttttttaaaa tgaatttttt tatcatagta
     3721 tactcttttt ttcatttaga acaatatgtt tcagtgattt tgacttgcgt tagtctagat
     3781 tctcctaatg tttgtaagat gacaaaggca ttgtaacaat tgaccaaaag aaccacgagg
     3841 acaattactt gaaaaataaa ggtcactttt tttggagaca aaaccctgct aaatcttgct
     3901 ccccacaaac ctccaataac agcagcgggg ataacataaa acaacataga taaatcaaag
     3961 cgcataaagc tacttgttaa agcaatcgtt accaattttg ccagttgaga aaagaaaatc
     4021 gtagatagag aatatagtgt tgcttctttt attggcaatg caaacatcag catcaacaaa
     4081 gaaacattga ctggtccacc accaatacct aaaaaactag ctaaaaagcc cagaaccccc
     4141 cccacatata aggtaccaag tccatgacgt caaatgaaat tttggctgat gatgcttggt
     4201 gtagaagaac gcaaaaacca aggtcaatac cgtcaaaaaa atttggatca gctgcacatg
     4261 cttctcgttt tcaaataact gtaaaaaaac ttcgaataca atgttgccga ctactcctcc
     4321 aacaacagct ccactagata cccaaccgac aatctgccaa ttcagtgatt tcccgcttga
     4381 tacttgtcgc acagtcgaaa caatggacat cgtgaatacc gcaactgttg agtaaaaaga
     4441 aatccctgca acagaatggg cgccgataag atcaagaatc ggtttgatca acacgcctcc
     4501 acccatcccg gaaatagcgc caactgtgtt cgccagaacg atcaccacaa aataaatcag
     4561 accaatcatc ttgtatttcc tttctcttgt ttatatcttt tagcgattgt tactcttttc
     4621 ttttattata cagtaaaaga aacaaacgag gcttcgaaag cttttctact ttttaaggaa
     4681 atttagtgct aaacgaaaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca
     4741 gccaaacaaa aatagctcct cataattttc gtttggttga acttattacc gcagatgcaa
     4801 gcttttgaac accgtttaag aaagaggtcg tgagcctgcc gttcagctcc gagtcacaag
     4861 gaacaatttc aaaaaacagc ttctcatgtt tgttgaatat tgtgacttgt gaccgaggag
     4921 ttggcaggag aacaccgttt aaggaagaga ctgtgaagtc aacgttttga cctgattatt
     4981 agagaaacaa atggcaaaat agttcttcat atttcgccgt ttttcgagcg aatgccgagg
     5041 ggcagctgat tgaacatcgt ttattt
//
LOCUS       DACDMM010000160         5053 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDMM010000160 DACDMM010000000
VERSION     DACDMM010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.162"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            42..2195
                     /locus_tag="IWD16_002360"
     CDS             42..2195
                     /locus_tag="IWD16_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP9089356.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2164..3579
                     /locus_tag="IWD16_002361"
     CDS             2164..3579
                     /locus_tag="IWD16_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP9089357.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3612..4646
                     /gene="sppA"
                     /locus_tag="IWD16_002362"
     CDS             3612..4646
                     /gene="sppA"
                     /locus_tag="IWD16_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9089358.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4648..>5053
                     /locus_tag="IWD16_002363"
     CDS             4648..>5053
                     /locus_tag="IWD16_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9089359.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQ"
BASE COUNT         1826 a          703 c          964 g         1560 t
ORIGIN      
        1 tttttctata aaaatttttt agaaggagtt gtgttagata agtgataaaa tcatttcctt
       61 ttgtgcagca acaagatgaa aaagattgtg gtgttgcttg cctttcgatg attttaaaat
      121 attttaaaac tgatatcccg attcataaat tacgggacct atctgggact gatttagagg
      181 gtacttcagc atttggattg aaaaagacat ttgaaaaatt gtcttttgat tgtatggcta
      241 ttcaagcaga tagtgagatc tggaaagaaa gagatcttat cttaccatta attgcacata
      301 tattggttga taacagctat atgcactacg ttgtaattta taaaacgaag ggaaacgtac
      361 tatggattgc agatcccgct aatggaaaaa atagaaaaac gattgaagcg tttcaaaaag
      421 aatggacagg tattctttta ttacctatgc ctagagaaac ttatattcct attaaagaaa
      481 aagtagctgg attaagttct tttttgccta taatttggaa acaaaaagga ttagtattcc
      541 acatcgtttt agcatcgatt ttcattacgt tttttggcat aggaagctca tattattttc
      601 aaggtattct tgattttttc atccctaatc aagcacgttc aactttaaat ataatttcca
      661 ttggcttgat cgctgtctat ttatttcggg tattgtttga atatagtcgt agttatcttc
      721 ttattatttt aggacagcga atgagtatgg caattatgct tcagtatttc aaacatgtgt
      781 taactttacc aatgaatttt tttgctacga gaaaatcagg ggaaattatt tctcgttttc
      841 ttgatgccag taagattatt gatgctttag caagtgctac gttatcaata tttttagata
      901 ttggtatggt gttgcttgtt ggggttacgt tagctattca gaatagtacg ttgtttttaa
      961 tcactctatg ctctttgcct ttttatgtag ttgcaatatt cgctttcgtg aaaagctatg
     1021 aaaaatcgaa cagggaagaa atggcagccg gtgcagctgt aaattctagt atcatagaaa
     1081 gtcttaaagg aatagaaaca attaaagcat atagcggtga agagaaagta tataaccgta
     1141 tagaccatga atttgttaaa ttaatgaaaa aatcatttcg tacggttacg cttgataata
     1201 ttcaacaagg aattaaacat ttgattcaat taattagcag tgcattaatt ttatggctag
     1261 gttcttattt tgttatggat aatagaatta gtctaggtca acttatcaca tacaatgctt
     1321 tactagtatt tttgacagat cctttacaaa acattattaa tttacaagtg aaaatgcaaa
     1381 cggctcaagt tgcaaataat cgtttaaatg aaattttcgc gattgattca gaaaaatcga
     1441 accagtcttt tgaatatatg ctttctgaac aattatttca acaagatatc actgtaaaaa
     1501 acgtttcatt ctcgtatagc atgaaggccc caacgttgaa cgaaatttca tgtacaattc
     1561 cttcgcacag taaaacggct atagttggag ttagtggttc tggaaaatca actcttgcaa
     1621 agttatttgt tcgtttttat gaagcatcag aaggatcgat ccattatgga aacattaatc
     1681 acttagacat tccttttcag atattacgcg atcgagtgac atatttgcct caagagtcat
     1741 ttttctttag tggaacaatc attgagaatc ttatattcgg tttagatagc ccaccaagtt
     1801 tcgaacaaat aatagaagtt tgtgagatag tccaactaaa tgaatttatt aatcaacagc
     1861 cattacgtta cgagacattg ttggaagaag gtggaaataa tctttcagga gggcaacgac
     1921 aacgattagc cttagcacgg gctttattaa aaaatccgga catattaatt ttagatgaag
     1981 caactagtgg attagatacc ttgttagaac atgcaattat gaattatctc ctcaatttaa
     2041 aagaaaaaac gattattttt attgcgcatc atttaccaat agcaaaagca tgtgatcaaa
     2101 ttttagttct ccatgaaggg catttggtag aaaaaggaac gcacgatgaa cttcgttttg
     2161 ccgatggtat ctatcaaaaa ttatgggaaa tctaggagag ggaaacaatt tatgcagaat
     2221 aataattggt tcgaacattc tagtatctat actaaacaac atcatagctt ctaccgttgg
     2281 gtgttatacc ctgtaattct ttttttatgt attattggat tgtttttaac ctttgctaaa
     2341 aaagaggttg ttattcggac aacggctcaa ttgaaccccg aaaaaataga gaagttacaa
     2401 gtgccattag aagctaaaat aatggaaaac ttcctaaaag aaaatcaatt tgttcataag
     2461 gacgatgtgt tggttagatt ggattgttct ttaatagaga atgaaaaggc tcagatagaa
     2521 caagaaaacc aacgtattac tcaacaaata aagatggctc agctatttat tgaaagtata
     2581 agtaaaggaa aaaatttgtt ttcaacggat gacagttttg gctacagtaa tcaattaaag
     2641 agcatgttgt cagaaaaaga atcactccgc tacgctttga agcaaagtga attaaatgat
     2701 caaaagcaat tagaagtata cgaaaagaca aaaagacaac tagaaaaaca aattgagagt
     2761 tcagatagta aattacaaga atggcaacaa gtacaggtag cttggagtaa taatcaatca
     2821 ttaaaagatt tttcaaaaga aatgatggca aactatgaga attggcaaga acaactaaat
     2881 aatgtttctg atgatcaaaa aaatcaagtg aaactgacaa tttcagcaag cataaatgaa
     2941 caaattgagc aactaaaaaa agaagtagaa cagtatcagt cagaaaaagc taaattagtt
     3001 aaaccaacta cttctgagaa tgacagaatt agtcaaacgg aaaaaggaaa gcaagagcta
     3061 gaacaagcaa taacaactgt aaaacaaaca gttacagagc taaatgaaaa acaagagaag
     3121 aaccagtcag ttatcaaaag tttggacgat caactttcaa aaggaatact aaaagctcct
     3181 gtatctggaa cggttcattt aaatgaagaa acaaagggac aagcagaact agccaaagga
     3241 acagtgcttg cagaaattta tccgatccac gaaaaatcaa aaatgaaatt tacagcactt
     3301 ttacctgcga atgaaagtac ctatattaaa gaagggatga aggttcattt taaattggat
     3361 caaaaaggtg gggctccgat tacgatcgac ggaactttag atgaaatttc agaaaattct
     3421 actgccactg agaaaggtgt tttttatgca gtgaaaggaa cgttaaaaac aactcaatcg
     3481 tttccgtttc gttatggatt aactggagag ttgtctttaa ttgtaggaga aaaaacatat
     3541 tggcaacaaa ttgtcgatgt gtttctaaat aaaaaataat tatttttact aagaatatag
     3601 gaggagttta tatgaataaa agaagatgga ttgctgtggg agtagcgtgc ggattattgt
     3661 tactatccat tatcgtcgcg ctaattccag gaaaagataa agaggaagca tcaaatacaa
     3721 ccctaacagg aataaataag attttttacg gatctaacga aatcacagaa gaaacgttag
     3781 aagaaggcgc atcaaataaa aagattgtca aactatccgt caatggagtt attgcagata
     3841 caggtgaaag caatcttttt agtagggaac aatataacca tcagaatttt ttaacacagc
     3901 taaagaaaat tcaagaagac aaagcggtta agggagtgtt attagaagta aattcacctg
     3961 ggggaggaat atatgaaagt gctgagattg ctaaagaaat ggcaaaaatt aaaaagctag
     4021 atattcctat ctatactgca tttaaaaata cagcggcaag tggagggtac tacatttctg
     4081 ctggttcaga taaaatattt gctacggaag aaacaacaac tggatcaatt ggtgtcatta
     4141 tttctggttt aaattattct ggcttattag aaaaacttgg cgtaactgat gcgacgtata
     4201 aaagtggtgc attaaaagat atgatgtcac ctcaacacaa accttcagag gaagagaata
     4261 aagttataca ggaatttgtg atgagtgctt atgatcgttt tgtcaatgtt gtagcaaaag
     4321 gaagaaatat ggatactaat gcagtgaaag aacttgcgga tggaagaatt tatgatggta
     4381 accaagcagt agaaaatggg ttggtggatc aaattggtta ctctgaagat gcattagatt
     4441 ccctaaaaaa agagaagaaa ttaactgatg cgacggttat agaatataaa aatgacacaa
     4501 ctggctttgc ttcttcttgg ttaggtaata agattgctga atggcaaggg ctaaaggcaa
     4561 ctacatcaga tcgtgtaatt tcagtttttg aaaaacttgg attttctgat actccaaaac
     4621 caatgtatta ctatggaggg ctataaaatg aataagaaga gtggcattga aactttgcct
     4681 aattatgtat ttgcgggatt ttggttacga ttttttgctt ttcttattga tttaatttgt
     4741 attagcatca tcacccatgc tactataggt ctcttttaca aaatcatggg actggaatct
     4801 gacaaatcca ttattacttt ttatggtata gcgtcattag ctatttattt aacctatttt
     4861 gtacttttga ctaagctgaa ttcaggacaa acaattggga aaatgatttt tgggataaga
     4921 gtagtgagct ttgaggaaaa agagctaagt tggcaaacta tctttattcg tgagtgtata
     4981 tgtaggttta tattaaaaaa ttttgtattt caaattgggt atttggtggc tgctttcacg
     5041 cccaagaaac aac
//
LOCUS       DACDMM010000161         5008 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDMM010000161 DACDMM010000000
VERSION     DACDMM010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.163"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(14..331)
                     /locus_tag="IWD16_002364"
     CDS             complement(14..331)
                     /locus_tag="IWD16_002364"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089360.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(358..564)
                     /locus_tag="IWD16_002365"
     CDS             complement(358..564)
                     /locus_tag="IWD16_002365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089361.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(545..727)
                     /locus_tag="IWD16_002366"
     CDS             complement(545..727)
                     /locus_tag="IWD16_002366"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089362.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(741..1091)
                     /locus_tag="IWD16_002367"
     CDS             complement(741..1091)
                     /locus_tag="IWD16_002367"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089363.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(1117..1419)
                     /locus_tag="IWD16_002368"
     CDS             complement(1117..1419)
                     /locus_tag="IWD16_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089364.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(1422..1655)
                     /locus_tag="IWD16_002369"
     CDS             complement(1422..1655)
                     /locus_tag="IWD16_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089365.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(2641..2963)
                     /locus_tag="IWD16_002370"
                     /pseudo
     CDS             complement(2641..2963)
                     /locus_tag="IWD16_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3086..4201)
                     /locus_tag="IWD16_002371"
     CDS             complement(3086..4201)
                     /locus_tag="IWD16_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089366.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(4207..4605)
                     /locus_tag="IWD16_002372"
     CDS             complement(4207..4605)
                     /locus_tag="IWD16_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089367.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         1398 a         1032 c          653 g         1925 t
ORIGIN      
        1 atttttgaag aaactatttt tctaatttct caatttcttc caagggattt tcgctaatga
       61 cccatacagg cacagattgc tctcgaggct ccccttcttc taaccaccaa cctgcataat
      121 gaacttcttc ctcctcagca ttaccatgat accaaccaac aaaggattca tctacaacga
      181 atgaaatttc ttcttctgga tcaaataatt catttttcca tgcttcaatt gcttgttctt
      241 tggtgtactt ttgtttgtta aatacaccac aaatttcgtc atctacataa aaaatttcaa
      301 aatcaaaatt actcgttcct tttactggca tcttaacttt tcctcctttg actcacttca
      361 atagatatac cactcatgtt cattccattg tttactagtt aattggagtt ttatcttagg
      421 gctttgtgca ctctttccaa aaacttgagc gtcgtttttt tcttctaacc aaaaaaggta
      481 atttcttaca ttcggtacta aatgtttttc ctgcatttct tcatgaaaaa catataattc
      541 tgcttcattt ttcaaccaat tcattattca ttccctcatt cacttcttca taccgccatt
      601 cagatagtga gatttctttt tgtttctcca cttgtcctat aaaaatcgct gtattcaaca
      661 agctgatcac acttaataat aaagcaatga aactaatggt tacacttact ctttcacatt
      721 ttgtcatttt gtttccttct ttactcgttt gttatttctt gtaataggtc taataactcc
      781 ttcaccgaaa tttgcaaatg gttcgcaaaa atttcaataa aagcactcgc acacattacc
      841 agaccttcaa ctgatatatc actgcgacaa taaaattctg gagaaacact gatttctgat
      901 tctttcttac aattgaacga aaaaactatt tttgaatcaa attccggtaa ttcttccaca
      961 actatgtttt gggattctac caagaaaggg agtgcctgtt ctaatgcttc ttctggtgtt
     1021 tgagcagata gaataattgc ttcttgttct tttatttcat tgttctcaaa tactttataa
     1081 ctttttttca ttgttagttc ctctttcatt cctctctcaa ttagctaagc cgttactggt
     1141 acgccattct ctaaatttag tatcgctaat ccttaatgct cgtcgaatat caatcattcg
     1201 ataacctatt gcacgaagtt ctttatatct tttagcagtt agcgtctcta acgtcatttc
     1261 aggcaatggg ttcgaatcaa aaatagaatt cgaaccgatt atttgggcca attgttgtac
     1321 ttgtaacagc tcttctgaac ttaattcagt gattgctcga ttgcctatca ttgcatacat
     1381 tttctgtcga atatacttct tttcttgttt agacaacatt tctatccact cctattttca
     1441 ttatcatttg ttacctccac ttcagtaaat ccacaaataa atgaattcat attccaagta
     1501 ccattccctt caactacaac ttttctcctg tcctctttcg gatattcaag aatcagaccg
     1561 tttaccaata tgtttttcac tactactaat ttgtttgtat accgtggaat tgctgttccg
     1621 atatacttca ctttatttcc tacgctaatt gtcatttcct cactcctatt cttttttatc
     1681 caaaactttt tcctgtccta ttcctgccaa cgaatatact tcactgcatg cttaaacaaa
     1741 acaaaatgtt gccctgtttt atttccgtct tttacttgtt ccacatacag ttcatatttg
     1801 gtcacacctt ttaaaacgcc agatactttt tttcatccat tgtcacaatt agttttttca
     1861 tcaatcgatt gatactgcac tgctcatttt tcttgttcat atagtattcc tttctttttc
     1921 aaaactagcc tcttttgttt actagttttc atcaatcatc aacgatttaa cagatttttt
     1981 ctttcgcagg gtttctagcg tcagcaaccc caaactctaa aaaagtgcct ttgctccctt
     2041 gaaaaacgag cggacgattg tactcgtcat catatgtttt tcatactacc tgatccacgc
     2101 aaataacctt tttttgttct gtgccacacc catccatacg tatcgcttga gattcaccat
     2161 acttttttag gtggtttatc cttcctaagt ccctgttaga cacgctgatt aacttagaaa
     2221 gtcctgtttg tatccaattt tcaaagatct cccaaaagaa ataagtaggt ttacgtgtat
     2281 cgcttctgtt caaagtacct gtttgtactt gagtatttct actcccctcg ttcaagggat
     2341 tccttccacc agctagcttt tcctttcatc atcaaatgtt cctgaaaagc atttacgttt
     2401 cgtgtccgtg tgcttactta ttcttttttc gatagcgatt atccgctcgc tactcgctat
     2461 tttatacttt gtttgttagc tcaaataaaa actaacaaac aaaaaaggct agtttctttc
     2521 cgttttattt ttttaactgt cgataaatag ttgttagttt aattccaagt tctaaaacca
     2581 atacgcccaa caaaaccaca ggaaaaacaa ttggggcaaa caagtaaaat cggatagttt
     2641 tcatgtcttc cctctttctt tttggtgtcg ctgtggtcgt tcttgccgtt caatacttcg
     2701 atacattttt tattcattat ttgctcgttt tgttttcctc tggactgctt ttcttgcttt
     2761 ttgaatactt tatacgcttt tcgtgcctga attaataggt cttgtcggcg tttcattcgt
     2821 gagaaaacaa tatttgaaac catcaaatcc tggttcgaac aaccacgatt cgcaataatt
     2881 ttcccatcaa aaaaaatagt tttcacttga gaaaaaaaca gttggaaatc ttgttttgtt
     2941 gctaattttt ttgtcaaaat cactgtgctt ttccctgttg aatctacaat gattcttcga
     3001 acaatccgat tcgataactt ccaacgttgc cgattaaaca atgttacttc acaacaaata
     3061 tgacgtaagt gattaggggt cattttcatg tgtccaattc ctccaaccaa ttatgcatcg
     3121 aaattttggg caattgtgac agcccataaa tctgcgtttc caggtcttca taactacaag
     3181 aacgcttggc ttgaagtaat gaatattctg cttgatctga tagttttgtt gccattggat
     3241 ctagattcaa ttctcttaca caacattccc aaaaattcaa tagcgctcta aaataaaatc
     3301 cttttcgatt ctgaattgct tttccttcat gacgagcaat tttttcattt tgaatacacc
     3361 gttgatacgt tttgcttaaa tcttctgcaa atgtttcccc taaaattcga tactcgcttc
     3421 catcagataa accatgtgtt tgattatact ttcgttgccg cttttctata attcgtttac
     3481 tgtcgtatat catgtttagc tgctctttcg cttcacgcca ttcagctgaa ctgcgaacaa
     3541 tccatgtcaa aacatctttc ggaacaaacg tactttcgct gtaatgagca ataaactctt
     3601 tttgccaatc atcagccatt tcaacaggac tagtcgtatc gatttttgta tctagatttt
     3661 ctgtatctag attgttagaa gaattattaa ataattgtgc agaattttca catggatttt
     3721 tttcgtcaaa atcatcgaaa atcgctaaat tatcaacaga ttcctcctct tcttttcctt
     3781 gtgcagaatc tacactggtt gagtcaacag gacctaatga atacacatca ttttctgtcg
     3841 tatctaattc tcctagatac aaccgagctt tgtttctttt tcctgtttgt tgatcaaatc
     3901 ccatatcctc tatttctagt agctcatatt ctttcaattc ttttttgatt gtattaacag
     3961 aacgtctgca acaattcaat gcattggcta attcttgttc cgtaaattca aaataaacaa
     4021 aaccttgact atctacttga tttcgttgaa tggctaaatc taagcgtgac cgaaaaacca
     4081 tatacgcgat ttttgcttta tcagacaatg agcgatattt ttcacttgta ataaatacct
     4141 ttggaaattg atagtataat tcattgtatt tacgagttgc ggtaaatctt tttgttgcca
     4201 tcgttattac acctcatctt gtccatatat taagaaagaa ttttggtgtt ggtagacacg
     4261 aacatcttcc attgtagcaa tcaattcttt tagataaatt cgaccgaatt caattgttag
     4321 taagtcgtaa tcttctaatt catttttgat ttcttcaatt tcttcttcag acacattgag
     4381 taaaatggct aattcttgat tggtataaga aatatagaca tgtttctttt catcaatccg
     4441 tttttcaatt aaggattctt ctaagtactc attcagtaag ctataggcgt attttgcttg
     4501 tgcagtcatc gcataatatt ttgacgattt tataaagaat agttttggca tatgaaaagt
     4561 tgcttgtgtc agcacatctg ttgcgcgaaa tttgtttttt accatatttt ttcctcctag
     4621 aaaaacacgc aaaaaaagct ctgtataaat cgaattacac agagtttctt cttgttgata
     4681 tttaatttct aaaatacgtt tcgggtaaaa actaaacact tactaatatt tagattgtag
     4741 aatcttcgcc ttcatttatt cattttaaag agaaaaagat caagtgaaat gtgcccaaaa
     4801 gtttgattct attaattttt aaacaccatt cataatatat ttcctataat gtaaaaagcg
     4861 tcgatactta ttaaagcatc gacgttattt ttatagtaaa tttatcaaaa tttttgccaa
     4921 cattttttcc gatttttttc cctatttcta tctttcctta tctttcttag ttaatattat
     4981 ttgatgttaa tatatcacta ctaatatt
//
LOCUS       DACDMM010000162         4987 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDMM010000162 DACDMM010000000
VERSION     DACDMM010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.164"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(254..1228)
                     /gene="sgrA"
                     /locus_tag="IWD16_002373"
     CDS             complement(254..1228)
                     /gene="sgrA"
                     /locus_tag="IWD16_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9089368.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1424..2263)
                     /locus_tag="IWD16_002374"
     CDS             complement(1424..2263)
                     /locus_tag="IWD16_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9089369.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2448..3335
                     /locus_tag="IWD16_002375"
     CDS             2448..3335
                     /locus_tag="IWD16_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9089370.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(3919..4614)
                     /locus_tag="IWD16_002376"
     CDS             complement(3919..4614)
                     /locus_tag="IWD16_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP9089371.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(4598..>4987)
                     /locus_tag="IWD16_002377"
     CDS             complement(4598..>4987)
                     /locus_tag="IWD16_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9089372.1"
                     /translation="FETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIELNQV
                     RKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVEDKE
                     MPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1584 a         1012 c          741 g         1650 t
ORIGIN      
        1 ctacatgaat aacgtacaaa tagattcgtt actgattttt cagagatact accatgcgca
       61 gtgtgcattg tcaattattc ttgctcttat tttccatttg tgttcctgtt atttcctgtt
      121 tctcaaatat gttagtaaac aaaaatagat ctggcaaagc tacaacttcg ccaggcaata
      181 cagcactctc gaatgaaata ttcactcaaa aatctatcgt tttattcaat tttagatctg
      241 ttgcttttat tctttatttc actcgatgga agagagaacg aaaatagctt gctcctacaa
      301 tcaaaatgag taacacaccc atccatgtaa ttagcttgtt caaggttcta ctaccagttt
      361 ctggtaaatc gctttttcta ttatctgttt ctttcgcttt ttcttgatga tcaattccac
      421 gagaaacaaa atcaggctgt tttagcacac tttttaggtt gctccctgca tttctctttt
      481 gttccttagt tggtatgata ggtaccaaat caagttcatt atttactttt cctgtgctgc
      541 tttcgctgct cgtgcttcct gtgctactct cactactcat gcttcctgtg ctactttcac
      601 tactcgtact ctccgtactg ctttcgctgc tcgtgcttcc tgtgctactt tcactactcg
      661 tactctccgt actgctttcg ctgctcgtgt tttctgtgct actttcactg ctcgtactct
      721 ctgtactgct ttcaccgctc gtgttttctg tactgctttc actacttgtg ctatccgtac
      781 tgcttggcgg tccaacaact gtccaactgc ttggtttcaa attctccgtg atcgtcatct
      841 gtccatcata aaaaacaatc aaatcaactt tatctttact cttgatctca tcaatcgata
      901 gttctgcaga aatcattatt gtatactcaa taccagcggt ccattgtacg tcttttaaat
      961 aaacttcccc aataactttt tctgaacttc cattaccatc ccaaccatat gttagaacag
     1021 catgtacatc actagcattc acgcttttgt ttggtttgac tggaatagta atgacttgtt
     1081 gtgtcatgtc ataaacaatc cgatcagttt tcacagtaaa tgaaagattc tcatttgccc
     1141 gttcatttgc agaagtactg attccaatca ttgctgcaat tatactcagc ccaagaataa
     1201 caaataatac ggtcgctgtt ttcttcattt ctatcactct ccatataatt accttctatt
     1261 tgataaagga attatataaa atgaaaatga tgagaacacg acagtcagtt gtgaaaatct
     1321 aacaaaagat gtcgtgtatt cgcttgaatt tgtttacttt ttcgtttaat caatgacaga
     1381 cttaaaaaag caagaccgat atcagccttg ctttcttcat cttttattga taaacagcag
     1441 tgtatttctt catccgcttt gttttatatg catagagtaa caaaccaaca atcaagacta
     1501 acaagaataa tgtagttgat atagatgctt cagccccaaa gtttccccca gtaattattg
     1561 tattttgcga ttggatcaga gtggagaaaa aaccttgttc ttgcgttccg ctgactggta
     1621 tacccaaaat agctccttgt gtccaattcc aagtaatatg agcaccaata gccgtaaata
     1681 aacttcctgt actgtagata agtgcgctga aaaagcaacc tgctaaaaag atattgaaaa
     1741 ctgccagaaa tgtcatgtta ggattcaatc cgtgcaaaag agcaaataat gcagcagatc
     1801 caatcattgc tattaacgga ccaaatctaa gcatctgatg ctgaaaaaag ccacggaata
     1861 aaagctcttc tgatgccccc actgcaataa ataaaataaa actcgttatc agcggcacaa
     1921 tgtttgcgtt tcctgaatct aaccgatacg cccccgtcaa atataatgga ctagtaataa
     1981 gcagaaacac tccaattcca acaacgagac ctcccgctaa atacccccag tcttttcctc
     2041 gaacagaaac accataatct tctattcttt ttccttcgac cttactagca agaaataaag
     2101 ctagtaaaaa aacagctaaa tgtccgaccg taccggcaat tgctccaatc cattcactat
     2161 ttccaataaa catatccgca agtgcattat tgacaagaat tcctaaaaca aaaccaacta
     2221 agactattaa aacagatcca atcaaccgaa taatcctgat catctttcat ccctccttta
     2281 tattaagttt aacaaccctt ttctttcttg tctcgtgaaa actatgtaaa tgttctccat
     2341 catagagaga atagattttt acgaactaag aatatctaca gcaaccttac gaaaatgaaa
     2401 gactgtctcc ctaattcttc tgttagactg tttttaataa ggaaaggatg ggaaattttt
     2461 tgaaaaaaca actactaatt atgtgccttt cagcatgtgc aattttacta ttctcaggtt
     2521 gtagcaagtc caatgaaacg attgcaacgt ttaaaggtgg aacacttaca gttcaggatt
     2581 tttatgataa cccaaaagtt aaatcaaata atcaaacact attaaaaaaa atgattgtct
     2641 tcaaagcatt tgatgatgta tacggaaaag agatttcttc aaaggaagta acaaaagaat
     2701 ataatgaaca aatcaaaaaa ctcggtccaa actacaagga acaattaaaa gcagttggcc
     2761 aaacagaaga gacatataag aaactcttta aacagatgct ggctttccaa tacggtttga
     2821 aagccaatgt aaaattaaca gataaagatc tagacactgc ttggaaagaa ttctatccag
     2881 aagttagtac acagatcatt ctattttcga ctaaagaaga agcagataaa gcaaaaaaag
     2941 aagcaaatga aggggaaaat ttctccaagc ttgttcaagc ttatggaaaa aataaaacat
     3001 taaaggaaac tgatggaaaa atgaattttg attcaaccaa tccagagatt cctactgaag
     3061 taaaaaaagc agcatttaaa ctaaaaaacg gggaagtttc tgatatcatt cctgtaacag
     3121 atccaacgac ttatcagcaa agctattatc tagtaaaaat ggtaaaaaaa caggataaag
     3181 gttctaataa agacaaatac aaatctgagc tagaaaaaat tgctaccgaa gcaaaaactc
     3241 ttgatacgga attcatggat aaaacaatcc gtaaagtgat gaaaaaagac aatgtaacta
     3301 ttaaagatcc ttatgtaaaa aatatattca aataaacaaa tttgacccaa ttggggatgt
     3361 ggcaaaattt gagaagtgat ttttggaaat tccttcttat ttttgaggca aaccactttt
     3421 gccacaactt catcggggcc tttttttatt tgtaccaaaa caaatcactg gatttgttac
     3481 aatcatgagg atataattta aaaaagtttt ttatttctat cacatgaatc aatgcgccat
     3541 taagtagatt ctttcatgct tccctatttt ttcacttgcc gtatcgcttc aaaaacaact
     3601 atcataaact caaactcgac tgaaaagtta ttttaaaaaa gactaaaaat atcccttctg
     3661 gacttgttcc tcttattctg ttatactaga gaaacacgaa agatattttt atgcgacact
     3721 tttcatttgt ttttatgaaa tttattcaaa actggcgtaa cttcagagtt acaaggatgt
     3781 attagaggtt agggaataca tcgtttaaat gagaaataag taatagtcac ccaaacttta
     3841 aattaatgaa tattgaatcg tcagatagcc tcctcgagtt ttcgagaagg ctatcgttta
     3901 ttttatggag aaattgattc agaagtaatc agcgaccttt tttagttcac caatcgtttc
     3961 tgcttggaaa gtttgcaccc aatcttcttg gaagcccctc actgctttat caatcgaggg
     4021 catctctaat acttgatcta gtagctcttt acctaacgtt atcgcatgag ctccagatcg
     4081 aatggctgta gtgacttgtt cacaattttt gaaactagct gctaatattt ttgttggtct
     4141 ttgcgtacgt tcgatctcag tccgcaacga atcaatcact ttcatagcat caatgccttg
     4201 attttgcatc ctgttataat aaggtgcaat ataatctgct ccattattaa tagctaaata
     4261 cccttgtaat tctgtgtaaa tagctgtacc ggtaatcaaa taaccttctt ttttcaactt
     4321 tttgatcgct tgaaggcctg cttgattcac cggcaccttg ataaacgtat cttttcccag
     4381 ttcttctatg ataactcgag catcctctat catttcttct tccgttcttc ctattgtctg
     4441 aatatggata ggaatctgtc cgattatttt tttgatttct ttcatatgcg agaagaagtt
     4501 tgacgctcct tcttttttca gaattgtagg atttgttgtt acgcctgtta aattcaaaaa
     4561 ctgttgacaa gaataaatac tttttgtatt tgctgtatca agcatgattt ccatatagct
     4621 ttcccccatt ctgatcctct tgatcgattt taatgattgt cccctttctt aaatgcggca
     4681 tttccttatc ttctacatac attgttcctg gaagttccgg ctcatgagat ccatcaaatt
     4741 ttatcgtgat atgtcctaat tcattcaaat tagtttggac aacatctcct actgctgtga
     4801 ttacatacat ttgtttatca aaacagagct tcatatcaca agttatttct tttcttacct
     4861 gattcaattc aatcttataa caataatctg ttaatgtttc cggtgcttca tctccaaata
     4921 aaatcattat tttttctttc ctgaatactt ctgcttctat accaacatct gttacaaccg
     4981 tttcaaa
//
LOCUS       DACDMM010000163         4948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDMM010000163 DACDMM010000000
VERSION     DACDMM010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.165"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..264
                     /locus_tag="IWD16_002378"
     CDS             <1..264
                     /locus_tag="IWD16_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9089373.1"
                     /translation="KAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKV
                     YNCFEELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
     gene            complement(434..1396)
                     /locus_tag="IWD16_002379"
     CDS             complement(434..1396)
                     /locus_tag="IWD16_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9089374.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1398..2837)
                     /locus_tag="IWD16_002380"
     CDS             complement(1398..2837)
                     /locus_tag="IWD16_002380"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP9089375.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            3022..>4948
                     /locus_tag="IWD16_002381"
     CDS             3022..>4948
                     /locus_tag="IWD16_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP9089376.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGT"
BASE COUNT         1588 a          844 c          822 g         1694 t
ORIGIN      
        1 aaagcttata gaaacaaatt gaaggagtac aaaatctttc aaagcatgtc tcggaaaggg
       61 aactgcttag ataattcact tatggagaat ttttttggct tattaaaaca agagattttt
      121 cacgggaaag tttataactg tttcgaggaa ttaaaatcag ccatcgatag ctatatctat
      181 tactacaata atgaacggat aaaacagaaa ttaaactggc aaagtcccgt ccaatttcgt
      241 aaaacaaccg taacagttgt gtaaaaatat cattttagag catgaaaaaa ggagtggatt
      301 tctccactcc aaaaaagtct aacttttggg ggtcactaca agttgacaac tcctttttgt
      361 ttacaaatca attggatatc tacaaattgt attttctaac gaattttata gacattaaaa
      421 aattaattta acgttaaata cttcctccag aaagtattga gattggtaaa atataatcct
      481 tatttgtctt ttcgccttta atttttttca gaagaatatc aacaataaga cttgcaattt
      541 catcaattgg ttgtcttatt gttgtcaatt gtgggaaaaa tttctcaata aaagaggtac
      601 catcgtatcc aataattttt aaatcttctg ggatattaag tttaagttga tgagcaattt
      661 tcatagtgag aatggctgtt aaatcatcag aaacaaaaat accatctggt ttgtgattta
      721 taatagttga ctttatttcc atttcacgac ggatagtaga cagattatta ggaattttga
      781 aaatttttcc atttggattt tgaaaagaaa aacctagtgc acgtaattct gtgggagaat
      841 ccgtgttatc atttcctgta atcataatgg tattttgaca accatttttt tgtaaagtct
      901 tagctgcaag ttttccccct tcaaagttat ctgaagaaac aatggaaaca ttaggagcta
      961 aatttctatc aaaagcaaca ataggtgctc tcacacgctc ataatcagca attccaagat
     1021 tatggcttga tgagattatg ccatcaactt ggttggcttc aagcatttca atgtattctc
     1081 tctctttagc aggatcattt tgactattgc aaataatcgt tttatatcca tgtttaaata
     1141 actctatttc gagatactca atgagttcag agtaaaagat attactaata ttaggaaaaa
     1201 taagaccaat aagctgaggt gactttcctt gaagtccacg tgctaaatta tttggtttat
     1261 aaccaagagt tttcatagct tcatgaactt tttgttttgt tttttctgat aaatatccct
     1321 tattattaat gacgcgtgaa acggttgttg ggctaactcc agctaattca gcgacatcag
     1381 ttaattttgc taccatttta atattttaat tcaaagtatt ttccagtagg ttcgcctgat
     1441 ttgatttgaa taccattttg gtgattttca gggaatacac gactagtaaa cactttttct
     1501 cctttattaa taaaaatctc aatgattgag ttatctacaa aaatgttagc agttgtttct
     1561 ttagtatcaa ttgagcattc acgaatcgta ccaaagtcag tggcatattg aatgcctgct
     1621 ttgctgcgat ctaaaataat ttttccttct ttggtatcta caatcaagct aagtccattt
     1681 cctttactat cagagaaaag aagtaactcc gatttctgat ttggaggaaa agttagttca
     1741 agctcataag tattatttgt ttctgctttt gctgtaaatt gttcagaact agcacgtaat
     1801 gatgtaatag cttctactgg atattggtaa agtttaccat cttttattga aagttctttt
     1861 acaagactca ttgctccctg ataatcatac ttatcagttg gataatcgat atcaggtagt
     1921 ccaatccaac tgacaatcag agcacgacca tcaggagtat tgaatccttg agtcgcatac
     1981 gcttcaaatc cataatcaag attttgaatt tctgaagcgc ccacaagttt tactttttct
     2041 atgtcaagcg attgacagat cttaaaagta ttcggataga tattcccata atttagctca
     2101 gatttatcca acccttgagg acaataaatg agaacaggtt ttttgtctac aaatactaga
     2161 tttggacact caatcatgta ttctgaacca gtgccgccaa aatcaaggtt gccaacttcc
     2221 tcccatgttt ccacgttatt gtcatccgct ttgtaaagtt tgataaagcc tgatttttct
     2281 aaattttgtg ctccaacaat cgcatacaat tgtccttcat aattaaacat ttgtggatca
     2341 cgaaaatgat cagttacatc agcaggttta tgaataagaa cattctcacc tttcgtaatg
     2401 ttgtagtctt tatccatcca tgctccgatt tgaagtgtat cacgttccca attttcattt
     2461 cgaacgtttc ctgtatagag taagaataga ttcccctcta tttcatatgc agaaccggaa
     2521 taagcgccgt ggctatctcg ttttgtgtct ggaagaagtt ctgctcctgt tacatgaaaa
     2581 tgaactaaat catctgattc attgtgtacc cattctttca acccatgagc agctccaaat
     2641 ggccaatttt gataaaaaag cgtatatttt ccgttaaaaa aagaaaaacc attaggatcg
     2701 tttaacaatc ctgttttagg ttcgatatga tagctagcac gccaaggtga tcgttttaca
     2761 ttatccttaa ttttatccat ctcatcttta gtccaatcag aataagcacg atatcgtatt
     2821 tctcgaggta agttcatatt ttactccttc aatttatctt tttacaacta gtatggtaaa
     2881 cgttttccgt tgaaaaatca atcatttcat ttaaaaaaat aaactatatg acaaacgttt
     2941 gacatatttt taagatatgc taaaataaat ttgcatttca gtgaaccgtt ttcacgtaaa
     3001 aataaaaaag gagtgattcg aatgaattac agtaaagtag cttccgacgt aatacaagcg
     3061 gtcggaaaag ataaccttat tgcagctgca cactgcgcta cacgtcttcg tctcgtacta
     3121 aaagatgaca cgaaagtgaa tcaaaaagct ttagacgaaa atccagatgt gaaaagcaca
     3181 tttaaaatta atggacaata tcaagtgatt atcggtgcag gtgatgttaa tttcgtttat
     3241 gacgaactta tcaaaaaaac tggactttcg gaaatttcaa cagatgattt aaaacaaatt
     3301 gctagtaaag ataaaaaatt caaccccatt atggcattaa ttaaattatt atcagatatt
     3361 tttgttccaa ttatacctgc ccttgttgca ggtggtttat taatggcatt acaaaacttt
     3421 ttaacagctg ctggactttt tggaccaaaa tcaattgaag aaatgtatcc tgcgattgaa
     3481 ggtatttcct caatgatcca actgatgtcc gctgcaccat tcatgttctt accaatttta
     3541 gtaggtattt cagctgctaa acgttttgga gcaaaccaat ttcttggtgc tgcaattggt
     3601 atgatcatga caacaccaga tcttggtggc tcaactgaat attggaatat ttttggttat
     3661 catgtttcac aaacaaacta tgcttatcaa gttattccag ttttagcagc tgtttggata
     3721 ttatctatat tagaaaaatt ctttcataaa aaattgccat catctattga ctttacattc
     3781 acgccattat tatcagtaat gattactgga tttttgactt tcacagttat tggccctgta
     3841 atgttattgt tgtctaatgg aattacagac attattgttt ggctatataa cactactgga
     3901 tttattggaa tgggtatttt tggaggaaca tattcactta tagtcatgac tggtttacat
     3961 caatcatttc cagctattga aactcaatta ctttcagcgt ggacaaatgg tatcggacat
     4021 ggagacttta tctttgtagt tgcatctatg gcaaacgttg ctcaaggtgc tgcaacattt
     4081 gccatttggt tcttaactaa aaattctaag acaaaaagtc ttgcttcctc tgcgggtgtc
     4141 tcagcacttc ttggaattac agagcctgcc ttgttcggag ttaacttaaa ataccgcttt
     4201 ccattctttt gtgctttaat tggttcagga attgctacag cagtagcagg attacttaaa
     4261 gtagttgctg tttcacttgg ttcagcaggt ttcttgggat ttctttcaat taatgcaaca
     4321 tctattccat tttacgtatt gtgtgaattg attagctttg tagttgcttt tgcaattact
     4381 tacttctacg gtaaaacaaa agctgctggt atctttaaag cagaagaaat tgctgaacaa
     4441 acaccagtta attcttcaaa agtagcaaca acagatccaa tatctactga tgttgaatca
     4501 acacaaacaa aagtttcatc agaaacaatt gttagtccca tttctggtaa agtgattagt
     4561 ctttcttcag taaatgatcc agtcttttca tcagaagcta tgggaaaagg gattgccatt
     4621 aaaccaaatg gaaacacggt ccattctcca gtagatggta ctattcaact cacgtttgaa
     4681 actggacatg cttatggtat taaatcaaat aatggtgctg aagttctaat tcatattggt
     4741 attgatactg tatcaatggg aggaaaagga tttacacaaa aagttactac taatcaaaaa
     4801 gttagaaaag gcgatattct tggagcattt gaccgttcaa tgattgagaa agaaggtctt
     4861 gatgatacaa caatgattat tgtgaccaat acttcggatt attcagaagt aaatatagta
     4921 gctgaaaata cagtcactga gggtactg
//
LOCUS       DACDMM010000164         4901 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDMM010000164 DACDMM010000000
VERSION     DACDMM010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.167"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(406..780)
                     /locus_tag="IWD16_002382"
     CDS             complement(406..780)
                     /locus_tag="IWD16_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9089377.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="IWD16_002383"
     CDS             complement(803..2203)
                     /locus_tag="IWD16_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9089378.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="IWD16_002384"
     CDS             complement(2207..2617)
                     /locus_tag="IWD16_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9089379.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="IWD16_002385"
     CDS             complement(2635..3126)
                     /locus_tag="IWD16_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9089380.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="IWD16_002386"
     CDS             complement(3136..3927)
                     /locus_tag="IWD16_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9089381.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="IWD16_002387"
     CDS             complement(3920..4693)
                     /locus_tag="IWD16_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9089382.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1556 a         1001 c          649 g         1695 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg g
//
LOCUS       DACDMM010000165         4884 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDMM010000165 DACDMM010000000
VERSION     DACDMM010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.168"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(110..1720)
                     /locus_tag="IWD16_002388"
     CDS             complement(110..1720)
                     /locus_tag="IWD16_002388"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP9089383.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(2913..4253)
                     /gene="glnA"
                     /locus_tag="IWD16_002389"
     CDS             complement(2913..4253)
                     /gene="glnA"
                     /locus_tag="IWD16_002389"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP9089384.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4281..4673)
                     /locus_tag="IWD16_002390"
     CDS             complement(4281..4673)
                     /locus_tag="IWD16_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9089385.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1439 a         1051 c          797 g         1597 t
ORIGIN      
        1 ttctatcttt tttcctacaa ataataacaa atcagaaaaa gagaaaagtg acaagaaaat
       61 tgcttatccc cttctcttgc tacttttctc tttctatcaa gttcaaaaat tattttgttt
      121 cgtgatctgc taatgatgct ccgacaaatg ctttgattaa tttttgcggg cgatttggac
      181 gagaaatcaa ttctgggtgg aattggcatc ccacaaagaa ttttttctct ggcagctcta
      241 cgatttctac taaacggtta tctggtgaga cacctgaaaa gactagccct tgttcttcaa
      301 acaattgacg atatttgtta ttgaactcat agcggtgacg gtgacgttct tgcacaacat
      361 cggctccatc atatgcttca gccgtcttcg ttcctttttt gattttacat gggtataatc
      421 caaggcgtaa agttccaccc aaattctcga tgttttcctg atcagccatt aaatcaataa
      481 tattgtttgc tgtatctgga ttcgtttcag ctgatgacgc atcttctaat ccaacgacgt
      541 tacgtgcaaa ttcgacacaa gccatctgca taccaagaca aatacctaaa aacggaacgt
      601 cattttcgcg agcataacga atcgcttcga ttttcccttc tactccgcga tcaccaaatc
      661 caccaggaac caagattcca tctgcgtctt tcaaacgttc ttctacattt tctctcgtta
      721 attcttgtgc tttgatccaa tcgatctcga tgtctgaatt ataggcgaaa ccagaatgtt
      781 tcaatgcttc aactactgag atatatgcat ctggtaactc gacatattta ccgacaagcg
      841 caatccgtac ttttttcttc aagttcaaga cacgttcttc taattctttc cattctgtca
      901 tgtctgcttc tggtgtatct agttttaaat ggtcgcaaac aatttgatcc attccttgtg
      961 cttgtaaaac taatgggatg gagtaaagtg tatctacatc tggcgactcg ataactgctt
     1021 ctggttcgac atcacaaaat tgtgccaatt tgtttttcac gctttgagaa acaggttgct
     1081 ctgtacgtac caccaaaata ttcggttgga tacctaaacc acgtagctct tttacactgt
     1141 gctgcgttgg tttagttttc atttctccag cagctttcaa gtaaggaatc aatgttgtat
     1201 ggatatacat aacattatct gtaccgacat ctgctttcat ttgacgaagt gcttcaagga
     1261 atggcaaaga ttctatatcc cctactgttc cccctacttc tgtaatgatc acatctgaat
     1321 cagtcatttt agctgctcgc atgatttttt ctttgatctc atttgtaata tgaggaataa
     1381 cttgaacagt tgctcccaag tattcgccct tacgttcttt acgcaaaact tcagaataga
     1441 tttttcctgt cgtcacgttg gagtatttgt tcaaattgat gtcaataaaa cgttcgtagt
     1501 ggcccagatc caaatctgtt tcagcaccat catctgttac gaatacttct ccgtgctgat
     1561 atgggctcat cgtccccgga tccacgttga tgtatggatc aaatttttgg atagttactt
     1621 ttaagccgcg gtttttcaat aagcgtccta aagaagctgc aacaatccct tttccgattg
     1681 atgaaacgac accgccggtc acaaaaatat attttgtcat aagttcaaaa actccttttt
     1741 aatttatgtg caggaaaagt ctcagacaat aaacaaagct ccctattctt caataagaat
     1801 agggagccga aatttctgta aacttctgac cctctaaaag ggtgcccaag gtgtataata
     1861 caagctgact aagggaaagt caagtactca aatgaatact atccgcttac aattttttct
     1921 ttttcgatat cctttgagaa attatttctt aagtccttta tgagtgcaaa aaaaatttaa
     1981 cccacataat aagcttgata taaacatagc taacgttaaa cctaagcaaa tcagaaaatc
     2041 gttactgaaa aattttaagt gaaataatag ataaaaaaat aaaacaaaca ttatgataga
     2101 taatatccca cctaacaaag aactactttt tcctacttgc agcaacagtt tatatagctt
     2161 atcgtagaaa caatcttttt ctttcaccct tatttacctc ctttacgtaa aaaacgtagt
     2221 ccacagttct cggatttttg gcatatcttt tctattaaat aatatcaaaa acaaaacaat
     2281 tccactaatt ttttcaaaga tgaccttttt ttcaacaaaa gtgccttgta gggattcatt
     2341 gaaggtaacg aatcacataa gtaaaaaata aggagatgcg acaaaagtcg tgcctcctta
     2401 tttctaaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa tatccatcaa
     2461 acatgagaag ctgtttttga aattatttct tgtgattcgg agttaaatgg taggctcaca
     2521 acctctggat aaacggtatt caaaagccag tttctcggta ttaagccaaa tttatgcaaa
     2581 aatgtagaaa gccattttca caaaattttt cttaatactc gaaactaaac gtctttttca
     2641 aaacctcttg gtaaacggtg ttcaaaagct tgcatctgcg gtaataagtt cagccaattc
     2701 aaaaacaaga aaagctgttt ttgcttggct attcttatta cttgttgcag ggcagctttt
     2761 tcacaacctc tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
     2821 ggcaaaatat gaggaactat ttttccgtta gtttctctaa tactcaggtc aaaacgctga
     2881 tttcacagcc tctaattcac ggtattcctg ttttaataaa gttctagata ttgttctctt
     2941 tcccactcgg aaacagtttg tctgaatgcc gcccattcga gacgttttgc ttcaacaaaa
     3001 tttgagaaga tatgttgtcc taatgcgtca atcattacat tatccttacg caattctttg
     3061 atagcattat ggatagttga aggtaagtcc tggatatgtg cagcttctct ttcttcctca
     3121 tccattacat aaatgttacg gtcaacagct gctggcggtg tgatctgatt acggataccg
     3181 tctagtcctg cttgcaacaa tactgccatt gccaagtatg ggtttgcaga tggatcaact
     3241 gaccgcaatt ccaaacgtgt ggataaaccg cgagattctg gtacacgtac taatggcgaa
     3301 cgattacggc cactccatgc gacataaaca ggcgcttcat aacctggaac gagacgttta
     3361 taagaattga ctgttggatt gcaaacggct gtgtaggcac gagcatgttt cagcaaacca
     3421 cctaagaaat gataagctgt ttcacttagt tccaattcac cgtctttatc atagaaaaca
     3481 tttccttcat cattgaacaa agacatattg caatgcatac cagaaccatt gatcccaaat
     3541 aacggtttcg gcatgaaagt cgcgtgaagt ccatgcttgc gtgcaatcgt tttgacaacg
     3601 agcttgaagg tttggatatt atcacaagca tctaccacat ctgcatattt aaagtcaatt
     3661 tcatgctgtc ctggcgctac ttcatgatgt gaagcttcca cttcaaagcc tagactttct
     3721 aattccaaca cgatgtcacg gcggcaattt tcacctaaat ctgttggtgc aaaatcaaaa
     3781 taaccacctt tatcattcaa ggtcgttgtg atcccaccgt cttcgtctaa tttaaataag
     3841 aagaattctg gttcaggtcc taaattgaaa gaggtaaaac caaggtcgcg catatctttc
     3901 aatgctcgtt tcaaattccc gcgaggatcg ccggcaaatg gtgtcccatc cggattatag
     3961 atatcacaaa ttaaacgagc aacttttcca tgagcacttt cccacgggaa gatcatccaa
     4021 gtggagagat ctggatacaa atacatatcg ctttcttcaa ttcgaacaaa tccttcaatc
     4081 gaagaaccgt caaacatcat tttattttct aatactttgt cgatctggct cactggaact
     4141 tctacatttt tgatcgtacc taagatgtct gtgaacatca atcgtaaaaa tcgtacgttt
     4201 tcttcctcaa tgattcgttt gatttcttca cctgttatct gtttctttac catggtatcc
     4261 ctcttccttt tttaatttat tcagtcaaag aattgtcttt gcaatccttg cgactgataa
     4321 tgacctgttt tgcttaaccc accttgcgcc aaaatctcat cacgaaaaat ttgtcgaacg
     4381 tcatgatctg tcaacggctt cttgtttttt gcttcttgtt ttttcgcctg tgccatttcg
     4441 tatacacgtt tgatcccagc catattcaat ccctctgaaa gatagtcttt aatctcaagc
     4501 aacatatcaa tatcattcag cgaatacata cgtcggtttc cttcgcttcg ttccggatga
     4561 actaaatctt gttcttcata atagcgaatt tgccgagcag acaaatcagt cagcttcata
     4621 actgtaccaa ttggaaatac ggacatagat cggcgcaatt ccttttctcc catcagacgt
     4681 cccctttcgt tttataagtt ggctaaagtg taccaattaa aaaaaatatt gtcaactgca
     4741 tatgttaggt tttctaacat gagatctcta ttttttataa aataacaaaa aaataagaac
     4801 acaaaaaagg tatctagttg attttcgtac cagatacctt ttatttttta ttatactgta
     4861 ctatccttta ttagtgtttt tatt
//
LOCUS       DACDMM010000166         4808 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.169, whole genome shotgun sequence.
ACCESSION   DACDMM010000166 DACDMM010000000
VERSION     DACDMM010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.169"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            72..1262
                     /locus_tag="IWD16_002391"
     CDS             72..1262
                     /locus_tag="IWD16_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP9089386.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            1396..3585
                     /locus_tag="IWD16_002392"
     CDS             1396..3585
                     /locus_tag="IWD16_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9089387.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            3582..>4808
                     /locus_tag="IWD16_002393"
     CDS             3582..>4808
                     /locus_tag="IWD16_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9089388.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT         1731 a          739 c          869 g         1469 t
ORIGIN      
        1 aatagaaaaa aggaagtccc ttctgtagaa tagagttacc acaacacatt cacagaagag
       61 aggacttccc tatgaacgat tttactacag aaattctaaa gactctagcg aacaaaggcg
      121 atttgaatga attattccgt gtccatttgg agaaagcagt caatacgctt ctcaaaacgg
      181 agttaacggc tttcctagat tacgaaaagt atgatcgcat tggttttaac acgggtaatt
      241 ctcgtaacgg ctcctatgac cgtacggtca agaccgagta tggggaactt catctccaga
      301 ttccgcgcga ccgcaatggt gagttcaagc aacagactgt tcctgcttat agacggacga
      361 atgacacgtt agaggagacc gtcattcacc tcttccgaaa aggtattacc atgtcggaaa
      421 tcgcagactt gattgagaaa atgtatgggc attactacac gccccaaacc atgtccaata
      481 taacaaaatc atttacagaa gaggtaacgg cgtttaatgg gcgggagctt catgaccgtt
      541 atgctgctat ttatatggac gcaacgtata ttccgttaaa gcggaaaacc gtcgccaagg
      601 aagctattca tatcgcagtt ggcattcgcc cggacggatc aaaggaagta ttgagctatg
      661 cgattgcacc gactgaatcc atcacgattt gggaggaaat tttattggac cttcaagagc
      721 gcggtttgaa aaatgtcctc ctgttcatca cggatggctt aaaggggatg gtaggagcga
      781 ttagtcggtt ctatcccaaa gctcgttttc aacattgttg tgtacacgtt tcccgtaata
      841 tcagtcataa agtgcgtgtc gatgatcgta aggaagtctg tgatgatttt aaaatggtgt
      901 atcaagcctc atctaaagag gtggcgttgg aagcacgtgg tacttttgcg gaaaaatgga
      961 aaaccagcta tccaaaagtg gttgaatcga ttctttcgaa cgatcacttg ctcactttct
     1021 atgatttccc cttggccata cgcaagagta tttattctac gaacttgatt gaatccttta
     1081 ataaacaaat caagaaatac agccaccgca aggaacagtt ccaaaatgaa gagtcgatgg
     1141 aacgtttctt agtctcgtct tttgatactt acaaccaaaa attcctaggt cgcagtcata
     1201 aaggtttcca acaggccgaa ggcgagctcg aacaaatgct aagccaactg attgagaatt
     1261 agaaacaatc tgcagtaggg aagaatcatt tacacaaaat tattgacgct cccttataat
     1321 gagaactttt actaacataa tttatgataa acttactata taatcatatt aattaaatag
     1381 ttaggagatc tgcttatgtt tatatctact cttcaaattc gaaattttag aaattatgaa
     1441 tccgaaatct ttcgattcga taatgagaca aatactatta taggtgagaa tgattcaggg
     1501 aaaaccaatg ctcttactgc attgagaatc ttattagatg atagttatta ctatagtagt
     1561 aaaacactaa aagaatcaga tttttttcat ggaattcaaa atgggtggca agggcattgg
     1621 attattatct ctgcgacatt tgaaggaatc agcgaagaag aatttgacaa tgagatttgt
     1681 gcttccattt ctttaaattc tgaaagtcaa acaatcttag aagaattgat ttcaaatgca
     1741 gataaaggtg taggtagtat ttctctattt attagaccta acaaagctat tagaaagcaa
     1801 ttatttgata tttccactga attagactct catcaattta acgaatttag aaattctatt
     1861 cgtctatccg actatgaatt ttactacaca tctaaatcaa atctaaattt ctgtatcaat
     1921 gaaaattatg ataaattggt aggaagactc aatgacaata tagctagtaa tcctgaagaa
     1981 gacgatgaag ctctgttggg ctcaagaatt gacatgtcag atattttcaa gcatatttcg
     2041 gttgtttatg ttgacgctct tcgagatgtt cttagagaaa tgaaaaacaa tcgaaaccca
     2101 gtcaaaagaa tcatggaaac aattgaatca aaaatatcat cagataatgt tgagtcatta
     2161 aaaacaatta tacagcaatt aaatgagact attaccagtg ttcctgaaat taggaaaatt
     2221 ggcgaaaata ttaatcggca actaaatagc ataattggtt cagtgtattc tcctaatctt
     2281 cttctttcct caactatgtc agatgatatg ggttctttag caaaatttct ttcaatgaag
     2341 ccagaacaaa atatcgactt agacttacta gggttaggac atctaaatat gatttattta
     2401 gctttgaaaa tagtcgaatt tgaagcgtgt cgttcaagag aactacttaa tattatgttg
     2461 attgaagaac cagaagctca tattcatcat catattcaaa aaactctgtt cgaaggatta
     2521 aatcttcaaa aaaactatac tcagattctc atgaccacac attctgtaca tttagctgaa
     2581 gtgtctgaaa tatctcggat gaatgtatta aaatcgttta atggcaaatc tatcgttatg
     2641 aatccagcaa ataatctgaa ctcatttgct aaagaaaagt taaaaaagaa caatctaaat
     2701 ctaataaacg cagtagagag atatttagat gttaaaagaa atggattgct tttttctaaa
     2761 ggtgttatct tagttgaagg agatgctgaa gaaatactta ttcctcaaat agtaaaaaaa
     2821 ttatttggta taaacttaga cgaacttgga ataggtttga ttaatgttgg tagtacttcg
     2881 tttgaatata tcgctagctt gtttgacgat gatagaatac agagaaagtg tgctattgta
     2941 actgatttag atgaacaggc aatcccatca gaacatcgat tgtataaaag cactgcacaa
     3001 gagaaaggtc aagcaagaaa agaaaaatta tcctcccttt ttaatgaaaa caattgggtt
     3061 aattgttttt atgctaatac aacatttgaa attgagtttt tagatgtgaa taaagaaaat
     3121 attctaactt atgtcagtcc aattatagat gacgcattca aagataaaaa aacaataact
     3181 gcttataaag aagaaataca aagtgaacaa tatttagaaa gaaatttagc gatgttaaaa
     3241 ctagccaatc atgttggtaa ggggtggttt gcaattgagt taggtgaaaa aattgacttt
     3301 ttaatcaaaa ttcctaagta tattcttaat gctattgctt ttgcatgtca ggaaatagta
     3361 gatctctcaa tttataaaaa aattctactc catacaatcg acttgtacga ggaaactaca
     3421 gaattgtcag ctttatcagc tagtttaaaa acaagtgata cagctgattt gaatatagca
     3481 ataatttctt tattagagta ctttggtaaa gatgaggatg ttgtgtatct aatcaatcag
     3541 atagaaaatt acacaaaaat actttatgct gaggtttcaa aatgacaaat atcttcgaaa
     3601 aaatcgtaaa tgatttaaat ttagaacaaa aagatgttgt ggaaagtgaa gataaccttt
     3661 atgtaaatgc atgtcctggg agtgggaaaa caagggtact tacaaggaaa attgcttacc
     3721 aagcaattaa acacgatgga tcactaaaaa gaataattgc tatcacatac actaatagag
     3781 ctgctgaaga aattaaggaa cgcctaagtt tacttagtat agatgatgat attaatattt
     3841 gggtggggac aatacatcag ttttgtcttg aatttattat ttatcctttt aaaatgaatc
     3901 tctcaagagt atcaaaagga tttacaatta ttgatgatta tactcaaaga gcttacgtca
     3961 gccaaattaa taatatgctg aatttaaatc tcaaaacgta tgaactgaac aaaattgatt
     4021 tgtcactttc tcatgatttt aaaattaatg aagttcagta tactcaagtt tctgaaatgt
     4081 atcacactat gttggaaaaa aataaagaaa ttgattttga tatgatttta ctagtagcgt
     4141 ataaaatttt aactgtaaac agtaacgttg cacgtaatat tgctagtaat ataagggcga
     4201 tttatgtgga tgaatttcaa gatactagag aattacaata caatgtaata gctaaattac
     4261 ttcaaaacaa ccctcaaatt cagtcaatgt ttgttggaga tattgaccaa gcaatttatg
     4321 gatcattaga tggtatagct aaatcagttg aagaactaga aatgctaact agtcacactt
     4381 ttcaatcaaa gacgctacat ggctgttaca ggtctaatca gagattagta gacttctact
     4441 ctaattttca gagttgtcct tacgagattg aaagcagagg gaataataaa aacgaccgtg
     4501 gttttataag ttatgattgc aaagttacta aggaggattt acccaacata ataaaaaata
     4561 taataaagga aaagattgaa agcggcattc atgaaaaaga tatttgtgta cttgctcctc
     4621 aacactatcc tctgttttca ctgggtgagt atttaaagaa agaattaccg tattgcaatt
     4681 ttgatgctat aaatatatca ccaattaagg taaataatca tagtttattt ttcaagttag
     4741 caattttgta ctttactgaa tcaggtgaaa aggttcgaag aagaaagcta attgcaaacg
     4801 atatttta
//
LOCUS       DACDMM010000167         4788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDMM010000167 DACDMM010000000
VERSION     DACDMM010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.170"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            564..989
                     /locus_tag="IWD16_002394"
     CDS             564..989
                     /locus_tag="IWD16_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089389.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2141..3118
                     /locus_tag="IWD16_002395"
     CDS             2141..3118
                     /locus_tag="IWD16_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9089390.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="IWD16_002396"
     CDS             3128..3625
                     /locus_tag="IWD16_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9089391.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="IWD16_002397"
     CDS             4001..4282
                     /locus_tag="IWD16_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089392.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="IWD16_002398"
     CDS             4284..4700
                     /locus_tag="IWD16_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089393.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          816 c          785 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtatacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DACDMM010000168         4761 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDMM010000168 DACDMM010000000
VERSION     DACDMM010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.171"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            104..1165
                     /locus_tag="IWD16_002399"
     CDS             104..1165
                     /locus_tag="IWD16_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9089394.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1168..2064
                     /gene="murQ"
                     /locus_tag="IWD16_002400"
     CDS             1168..2064
                     /gene="murQ"
                     /locus_tag="IWD16_002400"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9089395.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2078..4060
                     /locus_tag="IWD16_002401"
     CDS             2078..4060
                     /locus_tag="IWD16_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9089396.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4125..4571)
                     /gene="nrdI"
                     /locus_tag="IWD16_002402"
     CDS             complement(4125..4571)
                     /gene="nrdI"
                     /locus_tag="IWD16_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9089397.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         1553 a          827 c         1064 g         1317 t
ORIGIN      
        1 gaaatgaaat ttaattattt taataaaagt attgaaattt tgtttcttta gatatatact
       61 ctagtcatag aaatgaagac ggatacagaa aaagagggat aatatgtttg gcttttcaat
      121 atttatgaac gattctttgt cagaagagaa gaaacagtat atccgccaaa tggcatccaa
      181 tggctttgtc ggtattttca cttccatgca tataccagaa gatgatgttt cggcatatca
      241 aaagcgattg atcgaactag gaagcgaagc gaaaaagaat aaactagaat tgatggtcga
      301 tatttcagga gaggcattgg aacgagcggg tttttcatta gaagatctaa cgcctttgaa
      361 agcaattggt gtaacaggtt taagaatgga ttatcatatt tccaatcagc agattgccga
      421 ctggtcacat caattgaaaa tcagtttgaa tgctagcacg atcacgccta aagatatcga
      481 tgaattgaaa gaagcagaag ctgatttctc acagattgaa gcttggcata attattatcc
      541 acgtcccgaa acagggctgg acaaagaatg gtatcagaaa aaaaatcaat ggctcaaaat
      601 tcaaggtctc cttgtccaag gttttgtacc aggagataca gagttgagag gcccactata
      661 tcaaggattg ccaactttgg aagaacatcg agggatccat cctcttgcag gcgctctaga
      721 tctattagca agtaacacgg atattgttta catcggagat gcaggtctgt cagaaaacgt
      781 tcaggagcaa tttgcttctt tccaaaaaga acagacagtc ttgctccata cggaacctgt
      841 agatgaagaa ttttacgaat atatattagg gaagcatacg aatcggcaag acgatgcaag
      901 agatgtcata cgaagcgtag atgcacgttt tcgagagatt ccgcctatcc cagcaagaaa
      961 tacagctacg cgtatgaaag ggtctattac tttagataac gaaaagtatt tgcgctatat
     1021 gggtgagatc caattaacca aatatgattt gccagcggat gaaaaagtga atgtagtagc
     1081 taaagttatc aaagaagagc taccattgat caaccaaatc aaagctggta tgaactatca
     1141 atttataaga aaagaaggaa gataaaaatg agcaaaatca atttagagaa cctaacaacg
     1201 gaacgcagaa accaagaaac ttttggatta gatgagatga gtatttctca agccgttcaa
     1261 ctaatgaaca aagaagatca gaaagtggcc gctgcggttg aaaaagaact gacaagtatc
     1321 gaaccagtga ttgctaacac gattgagtcc tttaaaaaag gtggacgtct gatttatatg
     1381 ggagccggaa caagtggaag actaggtgtg ttagatgctg ctgaatgcgt accgacattc
     1441 ggtgtagaac cggaaatggt cataggtcta attgctggtg gagaagaagc aatgactgta
     1501 gctgtcgaag gggccgaaga ttcttatgag ctagggcaag aagatctaat caagctgaat
     1561 ttgacaaaaa atgatatagt tattggtatc gcagctagcg gtcgtacacc atatgtgatc
     1621 ggtggattag attacgcaaa atcgatcggt gctgtcacag gaacgatttc ttgcaataaa
     1681 caagccgaaa tcagtaaaca cgcggatttc ccaatcgaag tcgatgcagg tccagaattt
     1741 ttgacaggct ctactcgatt gaagtcagga acagcacaaa aacttatctt gaatatgatt
     1801 tctactatca gtatgattgg tataggtaaa gtattcaata acttgatggt cgatgtcaaa
     1861 ccaacaaatg aaaaactaat cgaacgctca aaacgaatca tcatgcaagc gacagaagct
     1921 gattacgaaa cagctgaaaa gtactttgaa gaagcagatc aaaatgttaa gctggctatc
     1981 gtcatgattt tgacaaatag tgataaagag gaagctcaag caaaattagc tgaagcaaac
     2041 ggatttgtaa aagggacctt atgaggggga aaagaccatg gcaaaaaacg aactgacttt
     2101 ggaagaactg gcaaaacaaa tttataaagg ggcaggcggc atgggcaatg tagaaagcat
     2161 tgtccactgc atgactcgtg tgcgtatgaa gatcatcaat gatgatcttg taaatgtctc
     2221 tgaattaaaa gcgattccag gagtgcttgg tgtagtagaa gatgaacagc ttcaagtcat
     2281 catcggacct gggaaagtga acaaagtagc acagcaaatg gttgatttgg caggcgtgaa
     2341 attaggagaa aaactttctt ctgtaagtac agctacagca aattcatcag catctggaaa
     2401 agaccgtgta aacgaacggg cacaagaaat gaaagcggca caaaaatcga aacaaaaacc
     2461 atctaaaatc aaatcggtat taaaagacat ttctaatatc tttgtgccaa tgattcctgc
     2521 atttgtcggt actgggatcg tggcagggat tgccgcagtt ttagcaaact tagtcacagc
     2581 cggaacactt gatgcagcaa catggcaaca atatatcgat gtgatgaaca tcttgaaaaa
     2641 cgggatgttt agctacctag ttatctatac aggtatcaat gctgcacaag tttttggtgc
     2701 aacaccaact cttggtggag ttatcggggc cgttgtgatg ctgacaggga tgaatccgga
     2761 agcaccactg aaaaacttat ttactggtga agctcttgca gctggacaag gtgggatcct
     2821 aggtgtcatc ttcgctgttt ggctattgtc aatcgttgag aaaaaactgc ataaagtagt
     2881 acctgatgcg gtagatatca tcgttacacc aactctttca ttgattgcca tcggtctgat
     2941 tgaaattttc ttcatcatgc ctttagcagg tttcatttct gatggcatgg tcggtgggat
     3001 caactgggta cttggcgttg gtggagcatt tgccggcttc gttctaggca cattattctt
     3061 gccgatggtt atgttcggac tgcatcaaat tttgactcca atccatgtgc aaatgattga
     3121 tgaaactggt cgtacgctat tgcttccaat tcttgcgatg gctggtgcag gacaagtggg
     3181 tgcagcattg gcattatgga ttcgctgcaa aaaagataaa gaattaacag aaatgatcaa
     3241 aggagcttta ccagtcggga tcctaggaat cggcgaacca ttgatttacg gagtaacatt
     3301 acctctagga cgcccattta ttactgcttg tatcggtgga ggtatcggcg gggcagtgat
     3361 cggaatgatc ggaaatgtcg gggctatcgc tattggacct tcaggagccg ctttgatccc
     3421 attgatctct gacggtaaat ggttaggcta catccttgga ttattggcag cttatgctgg
     3481 tggcttcgtt gctacattct tcttcggtat tcctaaagac cgtttagaaa aagaagaatt
     3541 agcgactgaa acagccacag taaatgaagt gcctgaaaaa gtagtacctc aaacttcagc
     3601 tgaaagtgtg actatcctct catcagttgc tgacggaaca gttgtttctt tagataaagc
     3661 aagtgatcca gtttttgcac aaaagatgat gggtgaaggc tactttgtag aaccagaaaa
     3721 cggacatatc tattctccag ttactggggt agtcagctct gtcttcccaa cgaaacacgc
     3781 tattgggatc acaacgccaa atggtttaga aatcttgctt catatgggaa tcaacacagt
     3841 tgacttaggc ggtacgccat ttgatgtgaa agtaacagaa gggcagcaag tgacacctga
     3901 tacgcttgta gcagaagctg acttagaagc gatcaaagca gcaggcaaag aaacatccat
     3961 gatcgtcttg gtaaccaata tggatcgtgt aaaaaacttt gtcttggaaa aaacaggtaa
     4021 agcaaaagca aaagcacctg tgatggatgt caaagcttaa tatgatagaa acaatagaaa
     4081 cgaaagaaga aactgaaatc tatttttcag cttcttcttt tttattattt attgttttct
     4141 gctaaaatct gataaattcg tgtcacatca gatggtgttc cccgtaattc ataatctgcc
     4201 aagaaaggaa agccaaatgt ttgtgcatat tgtttggctg ttagacaata ctgattattg
     4261 aagtttttat ttccgctacc gactacacca agacaaagtt tatgattgtc ttcatgttcc
     4321 agatattcac gcatcgtttc ggtcaagatt tctgtatcac cgttatctaa accatttccg
     4381 ccgtctaaat aagtaggaac gaaagtaaaa aatggttctg tttctttcgt aaaatcacta
     4441 ttttcatgga tttcttttaa agtaacttca ggattgacag gatcttctgc atgcattttt
     4501 tcagcatatt ctgctagatg tttggcaaag gcacgcgtat ttcctgaaat cgatatatat
     4561 aaaatgttca tggatcatcc tccaattctt tccttttagt atagcatctt ttttaagttt
     4621 tttggtggtg taatttggaa agggactagg cgctttgctt gtataatgag aaaaaagccg
     4681 gtagaattat atttctatta aagtttttat taattaagta aaaataaaaa aggtttgtta
     4741 tactttttga taaaagcggt t
//
LOCUS       DACDMM010000169         4750 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDMM010000169 DACDMM010000000
VERSION     DACDMM010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.172"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            367..555
                     /locus_tag="IWD16_002403"
     CDS             367..555
                     /locus_tag="IWD16_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089398.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="IWD16_002404"
     CDS             545..805
                     /locus_tag="IWD16_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089399.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="IWD16_002405"
     CDS             846..1073
                     /locus_tag="IWD16_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089400.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="IWD16_002406"
     CDS             1138..1710
                     /locus_tag="IWD16_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089401.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="IWD16_002407"
     CDS             1711..2022
                     /locus_tag="IWD16_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9089402.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="IWD16_002408"
     CDS             2013..2273
                     /locus_tag="IWD16_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089403.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="IWD16_002409"
     CDS             2434..2793
                     /locus_tag="IWD16_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089404.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="IWD16_002410"
     CDS             2790..4433
                     /locus_tag="IWD16_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9089405.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..>4750
                     /locus_tag="IWD16_002411"
     CDS             4468..>4750
                     /locus_tag="IWD16_002411"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089406.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAE"
BASE COUNT         1674 a          663 c         1008 g         1405 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga
//
LOCUS       DACDMM010000170         4742 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDMM010000170 DACDMM010000000
VERSION     DACDMM010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.173"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..258)
                     /locus_tag="IWD16_002412"
     CDS             complement(<1..258)
                     /locus_tag="IWD16_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089407.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="IWD16_002413"
     CDS             complement(274..924)
                     /locus_tag="IWD16_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089408.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="IWD16_002414"
     CDS             complement(935..2107)
                     /locus_tag="IWD16_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9089409.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="IWD16_002415"
     CDS             complement(2126..2998)
                     /locus_tag="IWD16_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9089410.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="IWD16_002416"
     CDS             complement(3020..3940)
                     /locus_tag="IWD16_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089411.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="IWD16_002417"
     CDS             complement(3940..4209)
                     /locus_tag="IWD16_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089412.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="IWD16_002418"
     CDS             complement(4206..4586)
                     /locus_tag="IWD16_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP9089413.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1234 a         1043 c          783 g         1682 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aa
//
LOCUS       DACDMM010000171         4718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDMM010000171 DACDMM010000000
VERSION     DACDMM010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.174"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..444)
                     /locus_tag="IWD16_002419"
     CDS             complement(<1..444)
                     /locus_tag="IWD16_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9089414.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSG"
     gene            complement(446..1114)
                     /locus_tag="IWD16_002420"
     CDS             complement(446..1114)
                     /locus_tag="IWD16_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9089415.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1244..1828)
                     /locus_tag="IWD16_002421"
     CDS             complement(1244..1828)
                     /locus_tag="IWD16_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9089416.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2159..2392)
                     /locus_tag="IWD16_002422"
     CDS             complement(2159..2392)
                     /locus_tag="IWD16_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP9089417.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2407..3357)
                     /locus_tag="IWD16_002423"
     CDS             complement(2407..3357)
                     /locus_tag="IWD16_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9089418.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3354..4196)
                     /locus_tag="IWD16_002424"
     CDS             complement(3354..4196)
                     /locus_tag="IWD16_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9089419.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(4198..>4718)
                     /locus_tag="IWD16_002425"
     CDS             complement(4198..>4718)
                     /locus_tag="IWD16_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9089420.1"
                     /translation="LVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKVFQRIKNAEW
                     QRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEPFVGIDLVSE
                     RIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVESVFNEENLKK
                     AYGDTIFIGEGE"
BASE COUNT         1609 a          856 c          583 g         1670 t
ORIGIN      
        1 accacttgat agcgaaaaaa tgtttttatc taatatattt gaaagatcta ataattcagc
       61 taattcattg attcttttat ccatatgctc ttttgtaaat ccaaagtttt ccatcgaaaa
      121 tactaactca ctcgttgtat ttgttgtaaa gaattgtcct ctaggatctt gacttacgta
      181 gccaatattt tttgatatac tttcttgtgt taaggattgt agcgagttat tttcaaataa
      241 aatttcccca accaattttc cgtcataaag tcttggaata agtccattca ttaactttaa
      301 tagtgtggat ttcccactcc cagaagaacc agtaatcaat gtaagactgc ctttttttat
      361 tgaaaaatta aggtcaacaa gcgcttgttc cttaacatcc ttatatgaaa aagaaacatt
      421 gttaaattca aacaattctt tcacctcaaa tcaaaatact taagaaaaaa atagtaacgg
      481 aaaacaggat gatatagtct aataagctca tgtttgtttt atagtaagaa cttcttttca
      541 caggagattc tatccccttt gttattgcat tagcggtcat ttcttcagca atcatagttg
      601 cacgtataag aacgggaact aacagaaatt ccaaatattg cagaggctga aaaaaagaca
      661 agcgaatgtt tctcattttt aaagattctt taatcattct tatttcagaa cgataagtcg
      721 gaaagaatct gatcactact gtaatactta acattatttt tttcggtaaa tgtatttttt
      781 ctaaagagga taataattca ttgctagtat agacactaac caacaaccat gagatcatta
      841 cgaccggcaa cattcgaaat acaatatata aggatgtcgc aaaaaatata gtaaaaaact
      901 ctatcttgat agcatcaata attttcatta ctaaaaacag aactgaataa atcaagatga
      961 aataaataca tgtctttatt ttacgaaaca tcaatactaa aactgtagca ataatgaaac
     1021 accccacttg aaatatattt ccctttccta aaaaaataat gaccggaaaa aaaatattta
     1081 aagtaatcaa ggttaacgga ttgatttttg acactctttc acctccaaaa aataagatcg
     1141 cactgttatc ttaagctgat aaaaatgcac cgttattcag ataacagtgc gatcttatca
     1201 aaaagcataa ctgagaacaa ttctcaatgc aagctttttt taattaactc agtaattcaa
     1261 tccacccagg aacagaaaat tgatacaatc ttctggttaa tagcatagtc ttttcttttg
     1321 aatagctatg cttcaatata tcatagaatg tagcgtagaa tgagtaaatg tagaagtgga
     1381 tttcttcttc attaaagact aaattttgat tgatcattcc gatagtcttt aacttatcta
     1441 gacaacagac tgtctttttc gtcacaaatg atgtaatttt ttcaacaata tcttgatatt
     1501 tggttccagc tgattggaat aaaatcaaat cgaatatacg tttattctca aaaatataat
     1561 taataaatgg atctaattca ttgaagttct ctctccaact tttctctata tctatatttt
     1621 ctaagtttaa gaggtatttg gtaaactctt cattatacat attaataagc gcattaatat
     1681 gcggctgaat aatttcttca aataacgctt ccttagaagt aaaatgttta taaaaagcac
     1741 ccgtagttac gcctgcggtc aggcacagtt gccttatctt agtttgctga aatccttctt
     1801 tttcaaaatg gcttagtcca ctttctatta acttttggtg agttataata taatctctgg
     1861 tttctatggg tacttcctcc taataagatg aattaacttt attatcttac acaggacttg
     1921 aagtttcaat cgttaacgtt tattactgaa ctcattcctt agagctaaac taatgagaaa
     1981 gagcagaata actgatacaa aaagcaatcc tacaagaatt aatagactca tctaaatcat
     2041 tcaccttttc taaaataatc cgagaaagat ttaaaaaaat tatatccagc tattaggata
     2101 agtcctatca taatcactcc aatagccatt ttcttatcct ccctaaaaat aaagtctttt
     2161 acttaaaacc ttttttgtaa tcgtcaataa attttttccc tttatcagtt aattgaaatc
     2221 ctatatctaa atagtcctca tatctaagtt tagccagcat ctcacttaca gatgctggac
     2281 taacacttag aacttttgct aaatcattat tttttaattt cttttttctt tttgataatt
     2341 cctgaattgc tagtaaataa attagtatag tagtcgtttt aatttgtacc atacctaccc
     2401 tccaaattat ttcgaaaggc cttcagcaat tttatccagg ttccatttca tcatcgcata
     2461 gtaactatca ccatcttgtc ctttttctgc aattgaatca gtaaaaatcg ttgaatagat
     2521 aggaatattg gtatcttttg atactgtttt cattggtcta tcatccacac tgctttctac
     2581 gaataaggag ggaacttttg ttgtgcgtaa tttttcaact aagtgtttta tttgatctgg
     2641 tgttccttct tcttcagtgt tgatttccca aatgtaagca gaaggcacat tatacgcttt
     2701 cgagaaatat ttaaagcatc cttcacttgt aacaatcatt tttttatcat tcggaattga
     2761 agcaaattta gatttagctt ctttgtctag agaatccaat ttttcaatat acttatctag
     2821 attttcttta tagaattttt tattatcagg atctttttcc gctaactgtt tttcaatatt
     2881 tttagcgtaa ataataccgt tttctaaatt taaccaagca tgaggatctt ccttcccttt
     2941 ctcactctga ccctctaagt aaataacatc tatgccatca cttgctgcaa aatagtcttt
     3001 gttttcctct ttgttcgcat ttttaactaa ttttgtaaac caagcatttc ctccagtttc
     3061 caagttaaca ccgttataaa aaatcaaatc tgcttttgaa gttttttgaa catcttcagg
     3121 caaaggttca tattcgtggg gatctttccc aataggtacg atactgtgta aatcaatttt
     3181 atcttttgct atattttcgg taatatctgc taaaatcgaa ttagtaacta ctacttttaa
     3241 tttttcgttc gaagccactt tatctttgtc tttactattt attgaacatg ccccgaatat
     3301 aaaaagagca atcaaaatcc ccagacttcc cagtactgct ttccattttc tcatcataac
     3361 gatctctcct tctttcttaa aaatttttgt tttggtgaga ttagaaaact aattacaaat
     3421 aagaatgccg ctgttaaaac aatacttgat cctgctgcta tgttaaaact ataaccaata
     3481 aacaatccaa gcaatgatga acttgctccg actgttgcag aaataaatat catagttttc
     3541 aagctatttg catataaata tgctgtagct gctggtgtaa ttaacattgc aacaattaat
     3601 atagttccca cactttgcat agctgttact gctactaaag ttaatagaac catcaataga
     3661 taatgataaa aactgacatt catccctatt gcttttgcca ttaatggatc aaaagaagtg
     3721 attaataatt ctttaaaaaa tagaactact ataaccaaca ctaaaaccga aacgccaatt
     3781 gtaatccact tatcaatatc ttgtacagct aaaatatttc caaataaaat atgaaataaa
     3841 tccgttgagc tatttgcaac tcctataagg ataactccta gtgctaaaaa agaactgaaa
     3901 gtaataccaa ttgctgtatc tccttttatt acactattgc tttttataaa agtaataatg
     3961 atagaagcta atagaccaaa tacaatcgca ccaataaaaa aattaatacc taaaatataa
     4021 gaaattgcta ctcctggcaa aacagcatga gaaatagcat cacccataag agacattcct
     4081 cttaaaataa taaaacaacc tactgttcca gaaacaatcc ctataactac agatgtaatt
     4141 agagcattct gtaaaaagtg aaagtcatac agaccattaa taaaattttg aagcatatta
     4201 ttccccctct ccaataaata tagtatctcc ataggctttt tttaaatttt cttcattaaa
     4261 tacagattct acacttccgt gagcaattag atttcgattc aaaagaataa tattatcaaa
     4321 atacttcttt accttgctta aatcatgatg cacaattagc actgtttttc cctgatgttt
     4381 caattttttt aacgtatcca taattattcg ttcactaact aagtctatcc ctacaaatgg
     4441 ttcatctaaa aatatgaaat ccgcattttg agcaagacat cttgctaaaa gtacacgttg
     4501 aaattgtcct ccagataatt ctcctatttg atgattagaa tattttccca tatcgacctt
     4561 ttccaaagcc tttgaaactc tttgccattc tgcattttta attctttgaa aaactttcat
     4621 tccggcatat gttcccatag atacacattc ttttactttt attggaaaag taaaatcaat
     4681 atcagctttt tgctctacat atgcaatctt caccagtt
//
LOCUS       DACDMM010000172         4710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDMM010000172 DACDMM010000000
VERSION     DACDMM010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.175"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            106..357
                     /locus_tag="IWD16_002426"
                     /pseudo
     CDS             106..357
                     /locus_tag="IWD16_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="IWD16_002427"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="IWD16_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9089421.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1765
                     /locus_tag="IWD16_002428"
                     /pseudo
     CDS             1654..1765
                     /locus_tag="IWD16_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="IWD16_002429"
     CDS             1786..1869
                     /locus_tag="IWD16_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9089422.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="IWD16_002430"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="IWD16_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9089423.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="IWD16_002431"
     CDS             complement(2994..3857)
                     /locus_tag="IWD16_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP9089424.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="IWD16_002432"
     CDS             complement(3859..4131)
                     /locus_tag="IWD16_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9089425.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="IWD16_002433"
     CDS             complement(4148..4363)
                     /locus_tag="IWD16_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP9089426.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4710)
                     /locus_tag="IWD16_002434"
     CDS             complement(4455..>4710)
                     /locus_tag="IWD16_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP9089427.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc
//
LOCUS       DACDMM010000173         4704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDMM010000173 DACDMM010000000
VERSION     DACDMM010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.176"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            74..1585
                     /gene="gadC"
                     /locus_tag="IWD16_002435"
     CDS             74..1585
                     /gene="gadC"
                     /locus_tag="IWD16_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP9089428.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1598..2998
                     /locus_tag="IWD16_002436"
     CDS             1598..2998
                     /locus_tag="IWD16_002436"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP9089429.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3163..4603)
                     /locus_tag="IWD16_002437"
                     /pseudo
     CDS             complement(3163..4603)
                     /locus_tag="IWD16_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         1480 a          774 c          886 g         1564 t
ORIGIN      
        1 tgcaagctca aataagcgat tttccataac cttggtagga atgacaaatt aaataggaaa
       61 gggaagtaat aatatgaata aaaaaaagtt atctctattc ggtttttttg ccctgacagc
      121 ttccatggtt ttgactgttt atgagtatcc gacctttgca acatcacaat tacatttggt
      181 tttctttctt cttttgggag gtattttatg gtttctacca gttgctttat gtgctgcaga
      241 aatggcaaca gttgaaggat ggaaagatgg tgggattttc agttgggtta gtcaaacttt
      301 aggagaacgt tttggttttg cagctatttt tttccaatgg tttcaaatta cagtaggttt
      361 tattacaatg atctacttca tactaggagc gctgtcgtat gtaattaatt ttccagagct
      421 taatacagat ccatttatca agtttattgg tgtattagtc attttctggg tactgacttt
      481 ttcacagttg ggcgggacca atcgtaccgc taaaattgct aaagcaggat ttgtcattgg
      541 aattgttatt ccttcgatca ttctctttgg tttagcagca gcctacttta tcggcggtaa
      601 tccgattcaa atggctatta atgcgaaagc ttttattcct gacttttctc agctttcaac
      661 tttagtagta tttgtatctt ttatattggc ttatatgggc gtagaagctt cagcatcaca
      721 tattaatgag ctggaaaatc cccaaagaaa ctacccattg gcaatgtttt tactagtgat
      781 tttggccatc gccttagatg caatcggcgg tttctcagta gcagcggtta ttccacaaca
      841 tgacttgtct ctcagtgctg gtgtaatcca aacatttgaa acgcttattt tacattttaa
      901 cagtcattta cattggttgg taaaagtgat tgctctcatg attgcctgtg gcgttatggg
      961 tgaagttagt tcttgggtag ttggtccatc acgagggatg tttgcagctg ctcagcgagg
     1021 tctgttacca gttttcttcc ggaaaactaa taaacaagac gttccagttc cgttgattat
     1081 gatacaagga attatcgtta ctatttgggc agcagtctta acttttggtg gaggaggcaa
     1141 caatttatca ttcttagtag caatctcctt aactgttgtc atttatctcg taggatattt
     1201 actatttttc ctaggttact ttgtattagt ttttaagaaa aaagacttaa aacgaactta
     1261 caatgtacca ggaaaaactg ttggcaaaac gattatcggt ggaattggtt ttattttatc
     1321 agtgttcgca ttgcttatct cttttgtacc accagcttca attgcagcta gtgagcaccg
     1381 aacataccaa atcatactac ttattagctt tgtagtgact gttcttttac cttttatcat
     1441 ttatgaagta catgataaaa gtgctcacac aactattgag gaacctactc acgtgaaagc
     1501 tgaagacgtt aatcccgctg tttatccagc tgctagagga gagcaccacg ttacaaaaaa
     1561 tgatgaacat attttaaaac attaggagga attcattatg ttatacggaa aagataatca
     1621 agaagaaaaa aactatttgg aaccaatttt tggctctgca agtgaggatg ttgacttgcc
     1681 taaatataag ttaaacaaag aatccattga accacgaatt gcttatcaat tagtacaaga
     1741 cgagatgttg gatgaaggaa atgcgcgatt aaacttagct actttttgtc aaacgtatat
     1801 ggaacctgaa gcagtgaaat tgatgaccca aacgttagaa aaaaatgcaa ttgataaatc
     1861 agaataccca cgaacaacgg aaattgaaaa ccgctgtgta aatatgattg ctgatttatg
     1921 gcatgctcca aataatgaaa aattcatggg aacttcaacg atcggctctt cagaagcctg
     1981 catgctgggt ggtatggcca tgaaatttgc ttggcgtaaa cgtgctgaaa aattaggtct
     2041 tgatattcaa gcaaaaaaac ctaacctggt gatctcttct ggttaccaag tttgttggga
     2101 aaaattctgt gtatattggg atgtggaact gagagaagtc ccaatggatg aaaaacatat
     2161 gtcaattaat ctagatactg tcatggatta tgtggatgag tacacaattg gtattgtagg
     2221 tattatgggt attacttaca ctggtcgtta tgatgatatc aagggtctga atgatttagt
     2281 tgaagctcac aataaacaaa ctgactataa agtatacatt catgttgacg ctgcatcggg
     2341 tggcttttat gcaccattta ctgaacctga tctagtttgg gattttcaat tgaaaaatgt
     2401 tatctcaatt aattcttcag gtcacaaata tggtttggta tatccaggtg tgggttgggt
     2461 cttatggcgt gaccaacaat acttaccaga agaattagta tttaaagtta gttacttagg
     2521 tggagaaatg ccaactatgg ctatcaactt ctctcatagt gcagcacaac taattgggca
     2581 atactacaac tttgtacgct atggctttga tggttatcgt gatattcacc aaagaactca
     2641 tgatgttgct gtttatttag ccaaagagat cgaaaaaact ggtatttttg aaatcattaa
     2701 tgatggatca gaattgccag ttgtgtgcta taagctgaaa gaagatccca atcgcgaatg
     2761 gacactatat gatttatctg atcgtctgtt aatgaaggga tggcaagtcc cagcctaccc
     2821 attacctaaa gacttggatc aattaattat tcaacgctta gttgttcgag cagactttgg
     2881 aatgaacatg gctggtgatt atgtacaaga tatgaaccaa gcaattgaag agttgaataa
     2941 agctcatatt gtttatcata aaaaacagga tgtgaaaacg tacggcttta ctcactaaaa
     3001 ttcttcagag aaatataaaa gaagttaatt aactaataaa aggagttgtg attaccacac
     3061 agctccttaa atctatttga ataaatatat gatattcatt tgttacactc tcaatcttag
     3121 ctgttgttct attcatcatt actcttattg taagtagttc agtcaaattt gcttgttcaa
     3181 ttgatcgata acagtatgaa ttgtttgaaa agtaatcgga tatataattg ttttctcact
     3241 acctaagcct tcttggtata aagaactatc gcaaagtata atccgctgag tacaaaagaa
     3301 agtgtcgtct actatttctc taagttgttt acaaatgata ttactaatag aaaagtgttc
     3361 tcccaaaatg tcttctaact gttgtcgata gtataaatac ctaaagtctg acggagctac
     3421 tatgaacact tcaattggag atttccgttt atgctcattt aaaagactta aactaatggt
     3481 tagtttacga atagaattct cattccaacg cagaaataaa ttgttttgtt ttcgccattg
     3541 ttcaagaatt tcatgaactt ctaagtacaa agcgcgttgc tcttcattta acaagtaagt
     3601 cttatcaggt tgaaatatct gaatatcagc ccaaagtgag cggataaacg ggagaagtgc
     3661 tttatcaaaa agtatctgat tttcttcaga tatattcaga gataagataa gtttttgact
     3721 gagctcaatc accaaagggt tatctgctac atggttttga taaacaacgt tcaagtcttt
     3781 ttgtaacaat tcaaagttat cgcatagata atttctagag ttgaatagaa agtagatata
     3841 aaggcattca tcttcgtcat gacataaaga gaaagaaagt tgctctaatc ctttctgtat
     3901 tagctgaaac aagggagtat gtcgggcaac tcttttttct tcctgactaa aatatattgg
     3961 gttagtctta gatctctgca aaccgatagc gatacctaat aaaataaatt gtttttcaac
     4021 tgtagggtaa cttcttgaaa aaaaatgttt ttctacataa tcaatcaatt gttgaccgct
     4081 ttgaataatt gatttattta gctggatcaa ctctgtccga tttgtctcat agattatcgt
     4141 tagtaacaaa cttcgataat ccttttcagg aatttcaaaa tggccatcag catttaagta
     4201 ctgcatttgt ttaaaaaaat taatcaactc atttcgtaag ttgtaaactt tgcttaacga
     4261 aatgaaggta gtatcagcta tattactcca attgtgttcc cctgataaat aatgtcctag
     4321 tacattgaaa aagtcagagc gctgatagat gagttgagtg agtgccaacc tcgaaaacga
     4381 agagtcgaat gcgactgtaa agctacttct agatttctcc actgaaataa tttgctgatg
     4441 gagtatgtcg gtaatgcatt ccaagtcgtt agtgacagac agtgagtggg caaacgttca
     4501 attgatctgc tatttcgcga atctgaatgg tccctttttc gtgtaattgc tcacaaatgt
     4561 aaacttgacg aaaaatactt ttctccagat aggtttccaa catagtactc ctcctagtca
     4621 ttttatacat aactattata actctaccaa tcggaatagt cctgtaatat attgaatcaa
     4681 aaatataaat tataaaataa ttag
//
LOCUS       DACDMM010000174         4696 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDMM010000174 DACDMM010000000
VERSION     DACDMM010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.177"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(22..684)
                     /gene="trmB"
                     /locus_tag="IWD16_002438"
     CDS             complement(22..684)
                     /gene="trmB"
                     /locus_tag="IWD16_002438"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP9089430.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(754..1533)
                     /locus_tag="IWD16_002439"
     CDS             complement(754..1533)
                     /locus_tag="IWD16_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP9089431.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(1740..2951)
                     /locus_tag="IWD16_002440"
     CDS             complement(1740..2951)
                     /locus_tag="IWD16_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9089432.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(2944..3681)
                     /locus_tag="IWD16_002441"
     CDS             complement(2944..3681)
                     /locus_tag="IWD16_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9089433.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            3819..4253
                     /locus_tag="IWD16_002442"
     CDS             3819..4253
                     /locus_tag="IWD16_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP9089434.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            4246..4590
                     /locus_tag="IWD16_002443"
     CDS             4246..4590
                     /locus_tag="IWD16_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9089435.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
BASE COUNT         1482 a          991 c          772 g         1451 t
ORIGIN      
        1 tatgaaagca gaaagttagt ttcacagact tttctttttg gtcttatctt gaaattgagc
       61 ttctactcga taaatacgtt gaccttttga agaaaatttt tcttcatact ccgtcataat
      121 attgccttca aattgacttt gatgtaaatc taaccatacc tgtttgagga tcatgccata
      181 ttgagaaaaa cttgccagag aatactcaaa caatccttga ttatctgttt tgaaatggat
      241 ttctccattt ggacgaagaa tctgttcatc tacagcaaga aaagtcttat atgtcaatcg
      301 ccgtttttca tgacgttttt ttggccatgg atcggaaaaa ttcagataga tttgatctac
      361 ttcgctgtca gcgaaatact ctgtcaacgc tgatccatcc acatgtagaa gtttcaggtt
      421 aggaagcggt tgttcgatca acttgtctag tgcaatcgaa acaacactga cctgcatttc
      481 tactccaata taattgattt caggatgggc ttttgccatt ccagtgatga actggccttt
      541 ccccatacca atctcaatgt ggattggatg atcgttttca aacaattcat tccattttcc
      601 cttccacaat gttggatcac taattacaaa attcgggtga gccgctaaca tttccgctgc
      661 tcccggacga tttcggacac gcattattta tctccattcc tttcctctaa ttaaaaaggt
      721 ctggccaaat ctccgcagag tttgggccaa acctcagcca ctgaaaattc gttttaattg
      781 taaaatttct tcgttcatcc gtcgatgatc ttcacgtttg caatatctgg tgatttcttg
      841 gagcaaattc atggccgcat accaatgaaa tttttcaagc atttcgtcag tcgggcgtat
      901 gccataactg atcagccact gattccagct agacagtggc acataatttc ctaaaatcgt
      961 tgcaatatcc accgcagggt ctgcaaacat gattgaatcc caatcgacta gatataaata
     1021 atttttacat actaaccaat tacgatggtt tacatctccg tgtactactg ataagttttc
     1081 cttatctatt gacgggatct gctcacgaag aaaacgatgg acaatttgca gaaattgatt
     1141 tttctggagt tcttccggta attctttttc atatttttgc agcatcttct ccggcgtcat
     1201 gacacgacca ccgatacggg tcagcatggt tttaagagaa tttgaatgat gtaaatggta
     1261 caaaacatca atcacgtcgt ttctttgacc aatctcatta ggagtcagca agcgaccttc
     1321 cagccactct tgtgctgtca aagtatcgcc attccccgtt cgttttgtcc aaacaagttt
     1381 tggtgtaatt ccttcccttg aaagagcagc caacatcgga gtcgtgtttc gtttaataaa
     1441 gactttgtcc tcatcacgca tgcctatgta tgtttttcct gtgtcgcctt tgatgggacg
     1501 taaccgccac tcttggtcta actgaaactc catcatgcat tctcccgtcc tcttactctt
     1561 tgttttgcag tacttattat tctagtcgcc tgaacactac acgtcaagca catatacgtt
     1621 ctttttcatt taattgccat tcaaaaaacc agcttttctc aaaaaaaata gaacgaacag
     1681 cattcttact gtcaacgctc tatttttcat tctcattttt accctacaca gctttgtttt
     1741 cagtctgaca tcttcttcaa acgagttggt acatatagtt taacaaagag catagtaaca
     1801 atcaaataag ccgctagcgg aacccatcgc tgctgcccat ctaatacgac tgcagcaatc
     1861 aaaccaaaaa taaccgacgc gataattaat acccatgtga gtagatactg aatcgcctgc
     1921 ttcttctgat tcttatggat tggataaagc tgaacgagaa ccatatagcg gaactggttc
     1981 gctaaaggta acagttggaa accgatcaaa tataaaaata agaaaccgat aatcagacta
     2041 aaataaaagt cgactactcc ggcaatcaat aacccaccta taattactaa tcggaaataa
     2101 agaccactaa aatcagaacc tcttgccaat cttcttgcaa acaagtaaag ataagtgttt
     2161 tttgattctc ttttaatatg agtcaatata ggatccaaat agcgtcttct tttgatcttt
     2221 gccgtaattt caggaacatc tgtgaataaa ttgataaatt gatagatctg atgcaaccta
     2281 tgttcttcta catgcaccat tttctcccaa tccaatcctt tttcttgttg cttccacaaa
     2341 aaccagtaat atagacttgc ctgtactaat gcacctgcta ttcctgccca tattagccaa
     2401 taaaatgata gaaacaaaaa gatcagagaa gaaaaaaacc agcctaattt ccaataacga
     2461 ataacttgtt gatctagtcg aaacaattct gctctcttga tttgcagatg actgaatttc
     2521 aaactccata atatgatcag atagacgaaa aacatcgaaa aggattgctc cgtactaacc
     2581 accactaatg gcatggccaa tccgcatgcc aatatcaaaa agatcaacgg aaaaatgcaa
     2641 gaatagcgaa aagctcgttc caaataggtt tgcatttctt tttctttagg aagtaaaaaa
     2701 acaatatctg ctgcctctgc aaaagtagcc atcttaccca cgaataaaca gagcatccaa
     2761 aatacagaga ttatgagccc tccaattgca aacggtggct gcagggtttt caaccaatta
     2821 gaataatata atcccacacc gccaatcaag aaaagacaaa ctaaaacaaa atgatcgttc
     2881 aatacataac gcatatattt catcattttc ttttgatgtc ttgctaaacg tttttgaaaa
     2941 aaactactca tgtgcctgct cctttgttaa agcaatatag atttcgtcca aagaagaatc
     3001 cggcagctga aattcttttc tcaacatctc aagagaaccg tctgctcgta tctctccgtt
     3061 atgtaagacc acaaaacgat cacaatattt ttctgcagta gctaaaatat gcgtagacat
     3121 caaaataccg gctccttctt ttttcatttc taccatcaat tcaagcaatg cattgatagc
     3181 tagtggatcc aagcctaaaa atggttcgtc gatgatatat aaagaaggct cgatcaaaaa
     3241 ggcgcataag atcatcactt tctgtttcat tccctttgaa aaattagctg ggaaccagtc
     3301 aagacgatta gataaccgaa aagtctccaa caacttttct gctcttttca ttgctatatc
     3361 cataggtata tcgtaggcca tcgcagtgac ttcgatatgt tctctcaatg tcaattcttc
     3421 atataaagaa ggagtctctg gaatataccc aatttgtttg cgatattctt ccgtattttt
     3481 ctttaaggat agatgattga tttctatcgt tccttttgaa gcttccagca gaccgataat
     3541 gtgcttgatg gtcgtacttt tccctgctcc gttcagtcca atcaacccta ctatctctcc
     3601 aggctgtaca tcgaaactga tatcttttaa cacagggaga tgtccgtacc cccctgtgac
     3661 atgatcgata attaaactca ttctttttcc tccgccattt atttttattt attataccac
     3721 gaagaaaatg acggtatttt gtttttttcc ttacaatagc tttaacttat ggtaaagtta
     3781 agctgaagta tttattcttt atagaaagga tgttttatat ggaaaactgt attttttgca
     3841 aaatcatcaa tcaagaaatc ccaagctata aaatatatga agacgacaag gtctatgctt
     3901 ttttagacat ctctcaagca acgaaaggac acacattagt tgtgcctaaa cgtcatgtag
     3961 cagatatttt tgaatatgat cccgaacttg caggagaggt tttttcgcgt gtgcctaaga
     4021 ttgctcaagc attggaaaaa gcttttccag aaatgaaagg tcttaatatc ttgaataaca
     4081 accgtgaact agcctatcaa tctgtttttc attcacatat ccatttagtc cctcgctaca
     4141 gcaaagagga tgatttttct attcactttg tcaatcatca agatagttat ggatcggaag
     4201 aactaaaagc tattcaagaa acaatcgtaa agcaggtgtc ctgcgatgat taaaaacttt
     4261 ttcaaaggac tgttatttgg agctgctgct ggcactgtcg gaggtctgtt attggcgccc
     4321 aggagcggga atgaaacaag aaagaaactg atcgaagaat tggaagaagc aacaactctg
     4381 acaaatgatt taaataacag cctgaatcat tttaaaagcg cattgatcga aacaaaagaa
     4441 acagctgaat ctgtgattcc tgctttccaa gaagcaatca aaaaagatat cgaagatttt
     4501 aaattccaag ccgaaccacg tatcacacaa atcaatgaac aagtagaaaa actaaaagct
     4561 gatctgccag agtatcctga acaaatataa taatgattat tttctcaaca gatttaacta
     4621 tataagtgag aaaagtttgc gtatttccta ggaatgtgat gaaattttga attttgtttg
     4681 aaactaccac gacaac
//
LOCUS       DACDMM010000175         4611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDMM010000175 DACDMM010000000
VERSION     DACDMM010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.178"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(86..340)
                     /locus_tag="IWD16_002444"
     CDS             complement(86..340)
                     /locus_tag="IWD16_002444"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9089436.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(451..705)
                     /locus_tag="IWD16_002445"
     CDS             complement(451..705)
                     /locus_tag="IWD16_002445"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9089437.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /locus_tag="IWD16_002446"
     CDS             complement(898..1173)
                     /locus_tag="IWD16_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP9089438.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="IWD16_002447"
     CDS             complement(1145..2098)
                     /locus_tag="IWD16_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9089439.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2710..4203
                     /locus_tag="IWD16_002448"
     CDS             2710..4203
                     /locus_tag="IWD16_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP9089440.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4338..>4611
                     /locus_tag="IWD16_002449"
     CDS             4338..>4611
                     /locus_tag="IWD16_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9089441.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1543 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2521 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2581 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2641 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2701 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2761 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2821 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2881 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2941 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     3001 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3061 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3121 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3181 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3241 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3301 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3361 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3421 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3481 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3541 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3601 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3661 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3721 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3781 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3841 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3901 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3961 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     4021 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4081 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4141 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4201 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4261 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4321 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4381 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4441 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4501 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4561 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga a
//
LOCUS       DACDMM010000176         4597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDMM010000176 DACDMM010000000
VERSION     DACDMM010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.179"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(86..1060)
                     /gene="lacD"
                     /locus_tag="IWD16_002450"
     CDS             complement(86..1060)
                     /gene="lacD"
                     /locus_tag="IWD16_002450"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9089442.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(1057..1515)
                     /locus_tag="IWD16_002451"
     CDS             complement(1057..1515)
                     /locus_tag="IWD16_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9089443.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(1512..2975)
                     /locus_tag="IWD16_002452"
     CDS             complement(1512..2975)
                     /locus_tag="IWD16_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9089444.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(2957..3889)
                     /gene="pfkB"
                     /locus_tag="IWD16_002453"
     CDS             complement(2957..3889)
                     /gene="pfkB"
                     /locus_tag="IWD16_002453"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9089445.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            4031..>4597
                     /locus_tag="IWD16_002454"
     CDS             4031..>4597
                     /locus_tag="IWD16_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9089446.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQ"
BASE COUNT         1378 a         1030 c          728 g         1461 t
ORIGIN      
        1 tatacttttt ttctataaaa tttgacaaac atttttttaa ataactaatt attttttgaa
       61 tctgtcactc agacaatcct ttctcttact tgttaacata aatagaagtt gctgtttctt
      121 ctaatagtgt acttaattct ttaatatttt gttttccttc ttctaacatc caactactcg
      181 ctttttcttc gccttcggta acaaaaggtg ctactccctt cgcccatgtt gctcgcccac
      241 ataagacacc attgaatgtc gaacctgatt gttttgcaaa acgcaatgta tcttgaaata
      301 gtgcagcgct gactcccgca cttaaaaaaa taaacggttg ttcagtcact ttcgattgtt
      361 catagaaata cgcggatgcc tctgcttgac tataacaagt atcttgccca tatccttcca
      421 caaatctcat attcactggt acttctactt tcaatacatc cacttgataa cgttcttttg
      481 aaaattctcg catggcttca atcactttat gcggttttac ttttgcatag tcaagagaag
      541 acggatcgcc aatagttgca tcgtaagaaa ggagctctaa aaagaaaggt atgccttctg
      601 cttcgcattc tgaaccaatc cgttctataa ctatttgttt ttgcacatta atctctactg
      661 gttcatcaac gtcatagtac aataaaaatt tacaagcatc cgcacccact tcttttaacc
      721 ttttcactga ccaactattc agtaaatctg gtagtcggcc tggcattttc gcatcatagc
      781 cggttttttc ataagctagc aacaaaccac attctttctc tcgaagcgct gctgcaggca
      841 gaccatattc tggatcgagt aaaatagcgg aagcatacgg agtcaggtac tgagaaacca
      901 tctgtttaaa ttggatgata tcttctgatg tagcttcttt atattggttg atcattttct
      961 tcaaagcacc acgttggtcg atagctagcg cgctaataat tccagcttca tccgtcaatc
     1021 gcttcaaacg ttcctgtttt tctatactca ttttcttcat atatcttcac ctatttcttt
     1081 agttaatatt gtagctattt ctatttttga ttttgcagct ctcaactgtt ctgtaacctc
     1141 ttttcgcatc aatgatcgtg caacgagaga caataatttc aaatgagtcg ttcctgcttc
     1201 tgaatctggg ataaataacg caaaaataaa atcaactgac tgtctatcta aactgttcca
     1261 ttcaattcct tttttcagtg tgacgataat caaactaggt tcattgattg tttgagactt
     1321 cgcatgaggg atagcaaaac ctgacattat tcctgtagta ccttcttttt ctctttccag
     1381 aagtttttga tatacttctt tttcgtcatg agcgattcct aaacagaatg tttgctttgc
     1441 taaaaactta aatgcttctt cttgattatc taatggctgt tgtaaaaaaa tgttctcttc
     1501 atgaatgatt gtcatgataa acctcctgct cttccttctt cgataactgt acgatttgtt
     1561 gctttattat ccctcttctt gtctttatca gttactcttt tcttttggat caaacctagt
     1621 atcactccac caacgatcga accaataaga atcgctgtaa cccattggat ttttcccgtc
     1681 actacaggta acactaaaaa accgccatgt ggggctggta cttggacacc aaacagatag
     1741 gtcaagacag cagcaattga tgagcctaac atcatcgttg gtaatactcg gatcgggtct
     1801 ttcgcagcaa acggaatagc tccttccgta atatgagttg atcctaaaat aaaattaacc
     1861 aaacctgcat tacgatcatt agtatcaaaa tatttaccaa acaataaagt agcaaaacca
     1921 gtaatcaacg gtggtgcaat gcaagctgct gacgcaccag ccataaaagt cgtgtttcct
     1981 tgtgtcaaaa gagctgttcc tgttacatat gccgccttat taaccggtcc tcccatatca
     2041 aacgcacaca tacagccaac aatcacgcca agaacaagtg gattcgaata ttcaaatcca
     2101 gctaaaaagc tcatcattcc ttcatttata gccttcattg gacctgccag caaagacatc
     2161 acgatgccaa taaatgctac accaataact ggatataaga cgattgcctt caagccatct
     2221 agttgtttag gtaaaattat cagtagcttt tgcaaaaata agataaaata acctgctaag
     2281 aacccagcta aaatacctcc taaaaatcca gtaccgccat tcatggctac gattccacca
     2341 acaaatccaa cgactaaccc tggacgtttc gcgatccctt ctgcaatata agctgctaaa
     2401 actggaacca tcattcccat tgctgcatcg tcaactgctt ttaccattgc cgctgtagca
     2461 ttgtattgtg catgatctgg atcagaagaa tagattcccc aaacggcaaa tgaaatagca
     2521 atcaatactc cgccagctac cacgaaaggc aacatatgag agacaccgtt catcaaattt
     2581 ttatagatgc tacgaccaaa attagcacca cttatcgctt ctggttctaa agtatcagca
     2641 gcttcattat ctgctaagac agtacctttt cctgccaatg cttcgtcaat cagccgttgt
     2701 gcatccttga ttcctgtact gacagaaaca tcaatgactc gttttcccac aaatctttct
     2761 gaatggacat ctttgtctgc ggcaataatg acagcatctg cctgtttgat ttcttcagcc
     2821 gttaattcat tctcgatccc aatttgtcca tgggtctcca ctttgatcgt gatgcctttt
     2881 ttcctagctg cttgctctaa tgcctcttgc gccatgtagg tgtgcgcaat tcctgtcgga
     2941 caaccggttg ctgcaattag ttgatatttt cccatctttc gaacctctct tccttgattt
     3001 tgatttgttt acttaattcc tccacatcca aaaaatcagt cagtccttta cgaaaagcag
     3061 tagagctgcc tgctgctatg cttttacgta acgtttcatc aagcgatcgt cgattcatgt
     3121 atcctgctaa aaaagtgcct aacatggcat cacctgcaca tgctgtattg acaacttttc
     3181 cttttggcga gtttcctcga atcaccctgt ctttcgtaaa aagaatcgct cctttctcac
     3241 cgagggataa caaaacattt tctgcgcctg actctactag tcgttgtcca taaacaaagt
     3301 aatcttcatc tgtaaccatt tttcgtccga accaagaaga aagttcttcc tcatttggtt
     3361 tcagcaaaaa cggtcgatag ggtaaacaat ccatgacttc ttgatcacta ctatcaataa
     3421 tcaaacaaat ccgactgatt tctattaaaa cctttggaga cagccctctt ggtaaactcc
     3481 cagaaacaca aagataatcc cctgcttgta atgctcttat ttgatttaaa aaattatgaa
     3541 cagctgtctg cggtatttca ggtccttgat tcactaattt gtattcttct cccgtttgat
     3601 tgacttgtgt aaatacattg atacgcgtca taccaggcac ttcaataaac tctgtttgga
     3661 tttgtttctg ctgtaaatag tcttctatat actttcctgt gaacccagca gaaaaaccta
     3721 atgccgtact tgggaaatct agcatcttta atactagtga cacattcacc ccttttccgt
     3781 ttgcctgaat gtcatcctct tttgtgcggt ttaccataaa aggatgcatc tcatctgttt
     3841 caataaatag atcaattgcc aaattcatcg tgcatgtgta gatagacata accaacgacc
     3901 tccttgattc tattatgaaa tacgcgaaat agaaaatgtt ttcttaatcg tcccaacaca
     3961 aagaaaacct ttacatttat aaactcgtcg tgcttttttt aactaacttt tggtgaaaga
     4021 aggaaagaat atgaatcaca aattaagtga aacagagcag tacctctgga actttatcga
     4081 acacctcatc ctagagatcc ctaactattc catcgtgaaa ctaagtgaac aagcaaatgt
     4141 ctcaacagcc accattgtgc ggacaatgaa aaagaaaggc tatgaagggt ttacttcgtt
     4201 taagcatcat ctaaaagaag gagaaaacaa gaacatcaat ttttcatttt tagataaagt
     4261 agacaaagga atccgccgag ccattctaaa aaatgaacaa gaagtggtac gtacaatcaa
     4321 tatgttggaa atcgggaaca ttgaagatgc aatacaaaga atcaaatttg ctgaccgggt
     4381 ttttgtcttt gcccgaggat tttcagaaat gattggccaa gaaatgctgg taaaacttca
     4441 attaacggga aaaaattgtc agatgcatac tgatccagaa attatcaaaa gcatcagtcg
     4501 gaaactaact aagaaagaca gcgttatttt tgtttctttg aacggtgaga aaacaaataa
     4561 aaacatcaaa aataagattt atgtctttat ttttcaa
//
LOCUS       DACDMM010000177         4595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDMM010000177 DACDMM010000000
VERSION     DACDMM010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.180"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            43..1977
                     /locus_tag="IWD16_002455"
     CDS             43..1977
                     /locus_tag="IWD16_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9089447.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            2434..2850
                     /locus_tag="IWD16_002456"
     CDS             2434..2850
                     /locus_tag="IWD16_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9089448.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            2864..3430
                     /locus_tag="IWD16_002457"
     CDS             2864..3430
                     /locus_tag="IWD16_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP9089449.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            3453..4439
                     /locus_tag="IWD16_002458"
     CDS             3453..4439
                     /locus_tag="IWD16_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9089450.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
BASE COUNT         1423 a          856 c         1010 g         1306 t
ORIGIN      
        1 atatattcat tatatagata gtctataagg agcggatgat caatggcaaa gcaaaatatt
       61 gtcgttgtag gagcaggata tgccggtgtt tcagctacaa aatacttggc aaagaaactg
      121 aaaaaagaag atgtagcaat tactctgatc gatcgtcatt cgtaccatac tatgatgacc
      181 gagttgcatg aagtagctgg cggacgtgta gaaccagaag caatccaata cgatctgcag
      241 catttgtttg ctcgtcaaaa gaatgtacaa cttgtcacag acactgtaat cggtatcgat
      301 aaagaacgaa aaatcgtcaa gaccaaatta ggttcctatc cttttgatca gctgatcctt
      361 ggaatgggcg gagagccgaa tgactttggt acacctggtg tcaaagcaca cggattcacc
      421 ctttggtcat ttgaagattc tgtaaggatt cgggaacata ttgagaagac agtagcaaaa
      481 gctgcattgg aaccagatga tgaactgcgt aaagccatgc tgactttcgt tgtctgtggt
      541 tctggattta ccggtattga aatgattggg gaactgatcg actggaaaaa tcgcctagcc
      601 aaagattata aattagatcc aaacgaaatc acactgatgg tcgtagaggc aatgccgacg
      661 atcttgaata tgttggatcg aaatgatgct gcaaaagctg aacgctattt gcgtaagcac
      721 aacgtggaat tattattgaa tgcaccaatc gtagaagtgg ctgctgatca tatcaaattg
      781 aaagacggac gcaacattcc gactcacaca ctgatttgga cagctggtgt caaaggaact
      841 tctgacgctg ctgactttgg attagaatca gcacgcggac agcggttgat cgccaatgaa
      901 tatatgcagg caaaaggcta cgaagataaa aacatctata tcattggtga tttagtcttt
      961 tatgaagaat tcccaggaac accgacaccg cagatcgttc aagctgctga acaaacagga
     1021 cacacagccg cagcgaatat cgtggcttcg atcaaaggcg gagaaaagca taaatttaaa
     1081 ggaaactatc aaggatttat ggtttctatt ggtgccaaat ggggggtagc taacctattt
     1141 gataagatcc atttaagcgg atttttagct atcgtaatga aacatatcgt gaatttgaaa
     1201 tacttctttg atattcgttc tggttattat atgttccaat atttaatgca tgagtttttc
     1261 catatcaaag atgatcgcaa cgtgactcga ggacattctt ctcgttacgg caatgtctta
     1321 tggagtgtgc cacttcgtat tttctatggt ttagtttggt tcgttgaagc ttccaaaaaa
     1381 atcattggga atggtgaagt gttcaaacca agtacttggt ttggtgaagg atcatggttc
     1441 actggaaaca gtgtgttccc ttggacatgg gaatacaatg taacagatgt tgccagtggt
     1501 gcttctgctg ctggtgatgc aaccacagct gccagcggag caggggcaag tgccggagaa
     1561 gcagcgactc aagctgcaca ttttggtttg agttatgcat acggtgaaga accaatggct
     1621 gtgattgaga agtcaccaga ctggttcaca aagatcatgc aagtgatcat gccaaacaat
     1681 gatgtagcaa tgttcttcca aaaattcatg gtattctttg aaattgcctt ggcattagca
     1741 ttgattgctg gattattcac atggctatgc agtgcgacaa cgattgtatt ggtgatcacg
     1801 ttctgcttat caggcatgtt ttactgggta aacatttggt tcatttttgt tgctttcgca
     1861 ttgatgaacg gttccggacg tgcgattggg ttggatcgct gggtaattcc atggctacaa
     1921 cgtacactag gtaattggtg gtacggtaga ccaagttccc agtacggcaa gaaataaata
     1981 gataaagaac gttatttgaa tgcaagattt caatagaaag gggctgagac aaaccgtcac
     2041 agcctccttt ttatgtaaaa ttaaaggaat aagatgcccg agataagcct ccatgagcaa
     2101 gacacaggtg tcacaacctc tgttacaacc tcttgttata aacggtgttc cagaagtagc
     2161 ttcttcgaaa aataagccga aatttcacaa aaacttgaaa agtaattttc gtaaattcct
     2221 tcttatttct tgaagttgcc cacttctgtc acaacctctt gttataaacg gtgttccacc
     2281 tgctgcttct cggtaataag cccaaagaat tagaaaacat ggaaagctgt tttctattct
     2341 tcgttcttat tactcaaagc aagacacagg tgtcacaacc tcttgttttt atgctaaaat
     2401 tgggtgagtt aatctggaaa aaggagaaca aaaatgagcc ttaaagaatt tgctgaaaaa
     2461 agtctgctca aaccttggga tgtttttatt attttgcttt tgactgttct ttctttttta
     2521 ccagtcatga tttttggtct tcagcaaaac accgcaacag cagaaaaaga agcggtactg
     2581 cgtgtggatg gcacagaaat caaaacattc cctttgatgg ctggagaaga gagcaacaca
     2641 tatacttaca aagatgaaca cggagattat aacttgatcg agatagatgg cgatcgagta
     2701 cggataaaag aagcagattg cgatgaccag atctgcgtac gtcgaggctg ggcaacgaaa
     2761 aatggcgaaa ccatcgtctg tttgccacat aaattagtga tcgaagtccg ttcaacagat
     2821 ggaggtgacg aagacagtct gatttattag accttcacac accatgagta aattacaaaa
     2881 aatgatttat atttcgctac ttgttgcaca aggagtgatc attggtttat tggaaaatat
     2941 gatcccttat cctttttctt ttgcgcctgg tgcaaaacta ggcttagcca atctaatcac
     3001 gatcgttgcc atttttacca tgaaaaaaca agacagcttc ttactacttt ggctacgact
     3061 attcctgact actttgctag gcggaacgat ttccacattc ctttatagta tgagtggtgc
     3121 tttgcttagt tattttggaa tgttattggt caagcaatta ggaccaaaac gtgtcagtat
     3181 catcggaatt agtgcggccg gcggtttcat gcataatgtc ggacagcttt tgatggcttc
     3241 tttcattgca caatcatgga cagtcatgtt gtacttgcca gtcctttcag ttttagggat
     3301 tttatcaggg attgcaatcg ggatcgcagc gaattatctt ttacgacatg tgcagacact
     3361 tcaacgattc caaaaagatt atgagcaggg aaccaaacaa aaatggagcc caatgtttac
     3421 caaaaaataa tcgtcacaca aggagttttt ttatgaagct acatccaatg tgggaaaact
     3481 atcctgcttt aagtaaagaa ttgacagaaa cactaaaacg gatggaagaa actgtccgcc
     3541 tgaaaaacaa acctgtcgaa caagccatca aagaaacgat ccatgccggc ggaaagctgt
     3601 tgcgtcctgc ctatcagcta ttgttctcac aatttggacc agagcaagat cgagacaaag
     3661 ccatcgcttt ggcagcttct atcgaaatgc tccatacggc cacactgatc catgatgata
     3721 tcgttgatga agcggatctg cgacgtggtc agcctaatat ccgtagtcag tttggtaatg
     3781 tcgtttctgt ctatgcagga gactatttat ttgtctgctg ttttaaacta ttgtctgatt
     3841 actccacttc cttgaaaagt ctccaattga attctcgcag tatggaaaaa gtattgaatg
     3901 gcgaattagg gcagatggat gatcgctata attatgaact ttctgtaaaa gaatatttgg
     3961 aaaatatttc aggtaagaca gctgaactgt tccaattaag ttgttctgtt ggcgcttttg
     4021 aaagtggaac aagcgagcgc ttcgcaaaaa aagcaggaga tatcggactt tctatcggga
     4081 tggcctttca gattatcgat gatattttag attatacaaa agagagccag gaaatcggca
     4141 aacctgtact agaagacatg cgtcaagggg tctattcttt accgttgatc tactctttgc
     4201 aaaaaaacaa accaaagcta ctaccttatt tagagaagaa agcggcaatg acagaagaag
     4261 atgtcgacgc cgtacgcaaa atcgttgaac atacaggagg cgtggaagaa gctaggaagt
     4321 tagcggcctc ttacacagaa aaggctttga aggaaataaa gaaattacct gccacctctc
     4381 ttcgaacaaa agagaatctc ttttccctta cccaactgat tttggatcgc aaagactgaa
     4441 tagggaaagg ttcgatacgt aaataaattt ctcagaagga aatctacgtg ttttttgaca
     4501 aattgaattg tagttcttgt atttatgata aaaagcaaaa ataaagagca acgtccgtta
     4561 gcgacgttgc tcttttttat cttgaatatt ttttt
//
LOCUS       DACDMM010000178         4502 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDMM010000178 DACDMM010000000
VERSION     DACDMM010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.181"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(251..754)
                     /locus_tag="IWD16_002459"
     CDS             complement(251..754)
                     /locus_tag="IWD16_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9089451.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1113..2138
                     /locus_tag="IWD16_002460"
     CDS             1113..2138
                     /locus_tag="IWD16_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9089452.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2461..3339
                     /locus_tag="IWD16_002461"
     CDS             2461..3339
                     /locus_tag="IWD16_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089453.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3815..3931
                     /locus_tag="IWD16_002462"
     CDS             3815..3931
                     /locus_tag="IWD16_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9089454.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         1476 a          727 c          819 g         1480 t
ORIGIN      
        1 ggaattcctt tctagattga gatattgact aggaagaatg ttaagatttt aagcctttaa
       61 gctaaaaaaa aacccttttt gttaaataag gggaatatag cctgtcagtt tcaaactgca
      121 agtcgtcctg ctgcaataat atagtagatt agagatgaaa atagattttt ttaaaccaat
      181 gactgaataa aaagaccgtc atagatacac attggaatgt gtctattacg gtcttttttt
      241 acgaacgatt ttattgaaga cggacagttc gggtcaaaaa taaaaaagct acgatcagaa
      301 ataagaataa aatgtatccc aaaataatgg ctagacggtc taacttattt gtcgatagat
      361 gtttgtttag aagttgatag gcccataaac ctatcagtcc tcctgttgta ttagttatca
      421 aatcagtgat gtccgctacg ccaataccga ataggaattg aatcaattct gccaagaagc
      481 ttacaaggaa aataatccct aattttttag aaaaagaaag tctcttgaaa ttgacattca
      541 aaagcacccc aaaaggaaaa aagacaatca agttataagt ggtctcacgt aataccgtcg
      601 tattttcaaa agaaaaagta gaaaaaggga cgagattgac attgctgtaa gaataagtga
      661 gaaattgtgg aagattaaat gatagcttga ataaaatcaa ccataataga aagaataaat
      721 aaatccctaa taaaaatttt gatacatacc gcatagacat ctcctccatt taaaaagtat
      781 accatcgaaa accgctgaaa aatcataaaa aagtatgaag tttaaggacc aacgtagtta
      841 tcgtacgaaa attagaagct tttttttgtt gggaatccgg tttctttaac aaagctaaga
      901 tagcgagttg cttggaaaag ttgctatctt ttttctgtca aaacgactcg gaatgttcgt
      961 tttttgttct aatttctcca agttgtttac tttttgatac taagtttatg aaaaatcaag
     1021 aaatagttgt tatactaggt aatgctgaca aacgggattg gcatttcatc ttggttcagc
     1081 tttttttcca attactagga aggagggata gattgttcaa gacttatttt cgtttcaata
     1141 aaatacaaga taatcctttt cgtatcatga atctgatgct acttatcttt attgtattat
     1201 ggttaggtta cataaatcat gattttttga tttcaagttt ttcatcatta gggatcttta
     1261 cttttttcta ttatcaaaac ataccgatga aatatttgct gaaacgtatg tttctgatcg
     1321 gttgcggact gttggttgct ttcgttttgg ggatcttatc gacttatgtt ttatggcttg
     1381 aaccatttgc cgtagctttg gttgcttttt ccagcagatt cgtgttgcga ttattccata
     1441 tttctaaacc aggtggcttg ttttttgcga tgctttctgc catgggaacg agcatgcagc
     1501 tgccaatcgc tcaattaccg atcgtctcac tttatttttt tatgggtgtc gtttttgccc
     1561 tgattgcggc agtgattaca aaattactgg attcacggcc tgagcagaca attgaaaaag
     1621 caactttaaa agaaagattt cacgaagagc ctttagtgat cattgattct gttttctaca
     1681 gcgctgcctt atttctttcc gtttatgtca gtcacggctt gaatctgcat aatccttatt
     1741 ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa cttggatgcg atgaagcatc
     1801 gacaagtcca atatctgatt ggatcgatgg gtgggttatg tgtgtcggca ttcttgtcat
     1861 ttgtgccgtt tacgcagttg cagaccattt ttctgatcac ttttctatat ggaatcgcac
     1921 aatttctagt tgctagaaat tatgcagttg ctaatatttt tcttaatccg atggcgttga
     1981 tgctatctac acttattcgt ggagcatact tgatttcttt gatcgaatat cgttttttag
     2041 ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg ggtaatgacg gttggtttgc
     2101 aacattattt aacggttgtt cgtcaaaaat tagaatagaa ggacaaatat atgtgccaaa
     2161 agaggttatg acaagatttt tgtcacaacc tctaattcaa ggtgttcaaa agccagattc
     2221 tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa ctttcaaaaa aattttatat
     2281 ttattctatc aaaaaatttt gtttatgatg gtaactgaat aaactttcct gagatggtca
     2341 atgctttcct tgaaagtctt acagatattc tttcttcata gaaagtttaa gttttatagt
     2401 tagtgtacaa taaaagtatg tttataccat atggtgtccg gataatcagt catttaaaca
     2461 atgaggttca aagatatgaa aaggggaaat ttgatgaaga aaataagcaa tgaaatccaa
     2521 attctggcag aaactttaac tcttcccaaa gtcgttatag agcaaatgat tgtacaagca
     2581 aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg aactaatgga tcctaataaa
     2641 gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag aggaaccttt tgcagcatta
     2701 gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa tttatcaacc attgaagatt
     2761 ccaagaacta tctatttggc aacaatgaat gcttttacta gatttattca tgaacatttc
     2821 caagtaacag gaagctatcg ctttgaccgt ggtttttgga tttggcatta cactagtggc
     2881 ttgatctttc ggattggtga attggagtac gaaatgacat attttccgaa agggtacacg
     2941 gatctaagat tagaaggaaa gcagtgcctt tctcttcata ttccttccga tgcagattta
     3001 tctcagaata agataagaga tagttacgaa agtgccattc gtttttttga actttatttt
     3061 cctgattacc attaccaagt gatgtacaca gatacttggt tactatcacc caatcttact
     3121 aaatggttga aaaaagagtc aaaaatctgt ctttttgcag ccgattatcg attattatca
     3181 gttgatgaac aggacgatag tggagtccct tggatctttg gacgagtaga tgcccaaata
     3241 catgattatc cagaaagtac gagtttacag cgacaagcaa aagagcaatt gcttgcaggc
     3301 gagcacattg gctctggctt aggtattctt acttcataag tttagtactt cgaaatagtt
     3361 taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
     3421 cggcggaagc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc
     3481 aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca gcctcttttt
     3541 tagtgttcta tcgaacacac agtacgttca ataggtaaac tagctataac aatagatatt
     3601 aaagttaacg gacaagaggg ccttttttag gtagctaaaa cgtaataatg aaaatttatt
     3661 tccagagata ggagtcaatc agctagtctg atcgactgtt ttaacaagtt gactttcatt
     3721 ttaacttttg ataccattta aaacacaaat cgtaattgtt tcgatttgtg ttttttagca
     3781 tgactacaat caaagaagaa agtgggtcgt ccatatggaa aaaacagata tctcaagtgc
     3841 ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa cgtgcgctga agatcatcaa
     3901 agatgtgaaa cggaatagtg ggaaaaggta agaagaagga aacttaatta aaaaaatagc
     3961 cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg tgtaaggttt ccgaaagaac
     4021 tgctgttgat tttccatttt taacatatga taaattggta gaatctttct tttcaaattg
     4081 ataccattcc ctaattccag ttatatagtt aactacagaa ccacaaagca cgtacgttta
     4141 taaaagtatt atctgtagtt atttttcaat tccctttcaa cctcttattt tgaccagtag
     4201 tattgatcac tttacctttt aacacctttg ttcgtttttg aaacattttt agtcaagaat
     4261 ggaaacgtaa ctacataatc aaaggttttg cgaaatattt catgaaatat ttcatgtaga
     4321 agcgagcaat acggttgcaa ataaccatta tcgtttaata agcagttacg aaagctccca
     4381 atatctctaa cgttcagctc actccatttc ttttcttcaa aaaaatcagt gattcgtatc
     4441 tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg ccgaaaaaat atgcaaaaaa
     4501 at
//
LOCUS       DACDMM010000179         4494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDMM010000179 DACDMM010000000
VERSION     DACDMM010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.182"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(384..830)
                     /locus_tag="IWD16_002463"
     CDS             complement(384..830)
                     /locus_tag="IWD16_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9089455.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            1425..1775
                     /locus_tag="IWD16_002464"
     CDS             1425..1775
                     /locus_tag="IWD16_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089456.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            1980..2828
                     /locus_tag="IWD16_002465"
     CDS             1980..2828
                     /locus_tag="IWD16_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP9089457.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            2875..3069
                     /locus_tag="IWD16_002466"
     CDS             2875..3069
                     /locus_tag="IWD16_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9089458.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            3072..4274
                     /locus_tag="IWD16_002467"
     CDS             3072..4274
                     /locus_tag="IWD16_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9089459.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
BASE COUNT         1583 a          834 c          762 g         1315 t
ORIGIN      
        1 tcgatagata aaaacagatt aagtacgagg tttccaatga attataaaat ccaataaaac
       61 tcagtaaaat gtgatttaaa agtatgttca agaccactca ccagtaagtt taatctattt
      121 agcaaaagaa gctatgtcta cactctttaa aatcaatatg ttttctcatt tttaaagttt
      181 caaaaaaact ctataactaa gaaaaatcac aaaaaagagt acattttcta gtgaaactgc
      241 accctttttt gctttgttta cgtacaaaaa atctcttgag tacttaactt ctacagtata
      301 atcaacaacc cctttataga ttactgatgt tatgctgaaa aaaatttttt caccaacttg
      361 taatctaagc cacttttctt attttacttc tcaaaccaat cataaaattc atctggtgtt
      421 ttctcccgat agagtttgat taacgctggt cgcatcgatt gaaatatttc gactaattct
      481 tccccaccaa atcgagtgga aaaatgctta tcagtaaata tcattgataa agaagaaaga
      541 aacagctccg tatcttcatc tgtaccaaga atattgatca actttccaat caaataggaa
      601 tcattaaatg tcaggtcttg tttcacatca aaggttttta ttgattcttt caccattttc
      661 gcaatgcgat ctttctctcc atacttgatc tcaccactca tctcgatata gtcaatgatc
      721 ccagatgaaa gaatcgtgac agcttcttct tttgcttttg atgatagttt gtttagcgtt
      781 ttggattctt caagtacctc tttcgtaatc tgtacgacat agtccttcat gatttcactc
      841 gaatattcta caaaacattc ttggataaat tcctccaaag gatagcctag ctcattctcc
      901 caagcaggat ttttgaactc gcccgtataa aattcttgct ttattttctc aatgatttca
      961 ggataatcgt ccactatttt tttctgacca atgagaaaca aatactctcg gatatattct
     1021 tccacatgcc caaaataaaa ccagccgaca ggttttccgc tgatactaga taattggttg
     1081 atcacactct taggagccaa cgcatagcct tgaacgtagg aactgatcgt cggctttttt
     1141 aaacctaatc gattccctaa ttctgtaaaa gagagagaca ttccgtcttt tatttctttg
     1201 attcgttggc ccaccttcac tttattaggt tgtactaaac caaacatatt caaattcctc
     1261 cttatcattt ttttcattat aacactcgta taaaaaatcg tatatatttt tttagaaaat
     1321 ctcttgacta cgtataagtt ctcgtatata atacaagtcg tagatggcca tctatatttt
     1381 ttaatcaaat cgtataaact ttcgtataaa aaaggagaat tgtgatgcca ataaaaatta
     1441 ccgatctcag tttaccaaaa gaaacactcg aaaaagaata ccaagtcgtc aatgtcactc
     1501 gctggcaaaa ggagggcgaa attctaggtt ggagctacga atgtatccta ccaaaacttc
     1561 gctacgaaaa aatcagtgtc aaagtcaaat ctgaacatcc tgtgatcacg ccggaagaac
     1621 ttacccaaca aggtttagcc ataattacat ttaccaattt ggtgattgta ccttggggac
     1681 gctcgaatgg acaatttgtc agttatgggt tatcggcaac cgctgatacg gcaactttga
     1741 ttcccagaaa acaaggtacc actacacaaa actaactata ctatggagtg ttgggtggtt
     1801 aggacgaaac ggcggggcag cgcccacaga gccgtttctg tccgttaaga ccgcccaaca
     1861 ccccataggc tagaagggct gctacgcagg ccttccccct taacttgatt tatagatcaa
     1921 catcaggaga aaatgactgg ttctttgaca atcgaatacg caacaaggag tgatgatttt
     1981 tgcaagctta taatcagaaa atcattgaaa ctcccacgta tattgaaatt tatgattatg
     2041 aagcaatcat tactcccaaa cgtaaaacga tcgaaaacga agtaaatctg gaacgaaaac
     2101 aaaaattagc ttggatttat gaaaatgata ttactcttga aaaaagaaaa aactatgatg
     2161 aattatccgc tcaaggacaa tatgacagtt taaaacgcaa acagaaacat tatcaaagta
     2221 tgcgatttga gattgcccgc cttgtagaca cgaattttga caatcaaacg aaatttttaa
     2281 ctctcacttt caaagaaaac attcaagaca ttgagtatgc aaataatgaa tttaaaaaat
     2341 ttatcaaacg actgaattat gacctctata aaactaaaaa agcaaattta aagtatctcg
     2401 ccacgtggga aaagcagcaa cgcggtgcaa tccactacca tattatcttg tttgatttcc
     2461 cctttatctc atttaaccgg ttaactacga tttggggtca gggtttcgtt aagatcaaca
     2521 agatcgatgt agacagtatc gaaaatcgcg ggcgctatat cagcaaatat ttcgataaag
     2581 agctggaatt gaaagagcat aagaaaaaag cttttttcaa gtcccggaat ctcaaactac
     2641 caactgtcac caaaaagatg gtaaagaaac cctatgattc cacgaatcaa gatatactat
     2701 atacaagcga atatatagtt agacgtccat ttttttacaa tgctttagct ttaaatgagt
     2761 tgggaagtcc tgaacaagtg atggattttc aagaaagtac cgttcattat acaaaaataa
     2821 agaaataaca ctattaccca atgatagtgt tttctttgaa agtgagttta tacaatgaaa
     2881 aatacaattt gtcgaaaaga tttagaggct ctaggattta aaacacatca agctaccaca
     2941 ataatccgac aagcaaaaac aagaatggta aatcagggat atccatatta taataataaa
     3001 agagttgggg tagtaccccg acaagtaatt gaagaaatta taggtgttcc tctttcacag
     3061 gaggaataac tatgaaaaca aaataccctt gtgtgtatca agataaaaat ggtcgctttt
     3121 cttatctagt tgagttagga acagatcaag tgactggaaa gaggatccag aagaaaggaa
     3181 caaaagattc tctaggaaaa cgattctcat cagctcgtga agcacacaaa gaagtcatgc
     3241 gtataaaaaa tgaatacatg gttgtaaatg gatttgcgaa ttatgattta acttacaaag
     3301 aatttatgga gcagtcttac gttccacact acaaagctag cgtacaaaaa agtacttgga
     3361 gttctcgtga gtgtggattg attcagatta ctgaattttt cggtgataag aagcttcgtg
     3421 aaatcaatgt gcgagattgt gaaaattatc gaatttggtt attgaacgat agcggatact
     3481 cacaagcata cagttcactt atgtacggta tgtttcgtaa atcattagac tatgcggtca
     3541 ccctgcaatt tctgaatgag aatatttcca aaagaacaaa agcaattcca aaaggaaaat
     3601 caatcgttcc ttactggaca aaagaagaat ttgaagaagt tttggcaaaa atttataaag
     3661 atgatttcta cgaacatatg tgttttttaa tgatctggct atattacatg actggtgttc
     3721 gtgtaagcga aggattagca cttaattgga atgacgtgga cttcaaacgt aaaaagttga
     3781 gggttcatca caccttggaa atgacaaatc aaaaagattt tattcggaaa ccttacacaa
     3841 aaactgaaag cggcttgcgg atcatttctc tggacgatga tacaattgaa gtgctaaaaa
     3901 agtggaaagt tattcaaaaa aatcatggtg tagaacaatt tattcttagc tacactgacc
     3961 tcccactcta tcgttcaact gttcaacgaa ttattcagcg ttatgccaaa ctagctaagg
     4021 tacccctaat acaaggaaaa ggattacgtc attctcatgt tagctaccta atcaatgagt
     4081 ttaacgcaga cattctggtc gtgtcgcaac ggttaggcca ttctagccct gaaatcactt
     4141 taaagcacta cgctcatcta tggagccgta atgatgaagg aattgcagaa caaatgacag
     4201 gaaacattaa atttacctgt gcaacaaaat ctaaagttgc aaactttaat ggaaatcaag
     4261 cagtaaaaat ctaaccctac catttcccta ccaaaatgag gaataggctg aaaaagcact
     4321 gatataatag cttctttacg aggctactca tattgagtgg gaataagacg taaatataat
     4381 atggatgggg aatgccgata aatcaacgtt tttcaacgta agcgccccat agaacagtac
     4441 cttactaaca aatgcacctc aagatatact tgagctatca agaagtctgg aggt
//
LOCUS       DACDMM010000180         4472 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDMM010000180 DACDMM010000000
VERSION     DACDMM010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.183"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            320..1051
                     /locus_tag="IWD16_002468"
     CDS             320..1051
                     /locus_tag="IWD16_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9089460.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1196..3223
                     /locus_tag="IWD16_002469"
     CDS             1196..3223
                     /locus_tag="IWD16_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP9089461.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3331..4428
                     /locus_tag="IWD16_002470"
     CDS             3331..4428
                     /locus_tag="IWD16_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9089462.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1571 a          717 c          923 g         1261 t
ORIGIN      
        1 aaatataata tacatttttt atttaattat aattaatatt gattgaagtc taggcagtca
       61 agtcacattt tgatagatag gagggggact tacgaattat tttaaaggct atgacagctg
      121 tatttctaga ttgtgtttgc atcaagttgc aataaaaact agaaatcaaa tcgggctgat
      181 aatcatttat aactaatcga tacataggac caaatatagg tctatgttct tttatttcat
      241 aagtatcgta aaacagaatt ttgctatgga ttaatccaat cgactgatat gtttagtaaa
      301 taaagaaaga ggaatgtcaa tgatgaaaaa agaaatcgta ttaacagcag caatgatgtt
      361 attcagcatg gtagcaagta cgacttttgt ttctgcaact gaagtatacc caaaggaata
      421 taacacagaa ggaacgatta catttgaagc aggagatgag ggtgtaactc ctcctgttga
      481 tccagagaat cctgatccca ataaacctgt cgatccatca gatcctccaa gccctggaac
      541 aggaggggca ttatcgattg attatggatc aaaattcaaa tttgggactc aaaaaatttc
      601 tactgcggat aaaacgtatt atgcagctgc tgatgtgatg aacgatgggt ctagaaaacc
      661 gacatatgtc caagtgacag ataggagaag taccttatct gggtggaaat taagtgtctc
      721 tcaaccagaa cagtttaaaa ctgccagtgg ggatgaacta gttggagcac agctgaaatt
      781 tacgaaaggg caggctgttt ctttggttga tccaacatat acgccgcaaa cggtgaattc
      841 tgaattaacg ttgacaccgg gaggaaacaa tacgttagca attaatgcta aatcaggaac
      901 aggtgtgggg acttgggtct atcgatttgg tgcaaatgaa aatgaaaatc aagatgcggt
      961 acagctttcg gtacccggta aatctgtcaa gttagcgcaa caatattcca ctaaattagt
     1021 ttggacatta gaagatacac ctaacaacta gtttactgaa aagaactgtt aaaaataaat
     1081 acgtagtcat attgtcttgc gaggtaatcg agcaaaaatt gtcaaaggct aggctaaatt
     1141 tgtttggata cgaatagttc atctaggaat ttaacgatca ggaggaaaag atatattgaa
     1201 aaagaggagg atcgtgctca ttttaagcag tctcttagtg actaatatct ttccgatcat
     1261 agggtactct ttggaattga atcagttgga gcatggaaat agaagagacg aagaaagcaa
     1321 actcacttcg acattcgaca gtttaataga atccaaccag acacaagaaa gcagtgagga
     1381 tttaagtatt tcgagtagtg gagagaccac tgacaacgta gaagctgagg gttcggacaa
     1441 acaaggacta gaagatgcac caatttctgc ggttgagctg cctgacgatc ccaatattat
     1501 ttctatcgat aaaatttttc aaacacctat cggtactagc acgtccatat tggaaaatgg
     1561 gaagttactg cagttgaacc cagcagaaag gtctcaaaga ggagctattt ggtcaaataa
     1621 gaagattagt ttactctcgg attttacatt taaatcttat ttatacttag gaaatcaacg
     1681 agatagagcg ggagatggca tgacctttac attgactggc gatcctagaa tggctagtaa
     1741 tcccagtcaa gttattggat cgccaggtat gggaattggc tcatattcta ctcgttctgg
     1801 ccaaccttac attgcgaatg cgctctcgat tgagtttgat acgtataaaa atagtggttc
     1861 ttctgatcga atggatcgag agataactgc tgataataga gggcatggac atgtcgcttt
     1921 tgtcacgcct aaaccaaata ataataatta ttctggagag catactggcg tgacggtagc
     1981 tcctacattt ctttctaatg gcacatggcg cttgttaata attaaatggg acgcggagca
     2041 aagaagtctt acttatgatt tggaaggtgt cggtcactct tctcggatta tacctgatct
     2101 cattaggcag tttggttcta cagaagtaaa ttgggggttt acttcttcaa caggtacttt
     2161 ttttcaagaa aatgctgtcg ctgtcacgcg acttccatcg aatgtcacat caacagctga
     2221 tgtaaaagtc aacgatagtg attatggggc catcgctgaa gctggaggaa acgataagat
     2281 aactatcaga aatacattga aggtcgaggg ggattttctt gaatataaga aaatgcagac
     2341 tagtatcgac ctcccacaag aattaactat tcccgagcat atcttgatca atggaaatca
     2401 aattgatgcc aaagatatct tgataaccgg aaatcgagtc acgctaaatc tgacagatta
     2461 ttttattgct ggagatacgt taataatcga attggagaca actttgcagg aagtaattcc
     2521 tgaaaaagta ctcactatgc attttgaata ccttgaaaac gggaatttga ttgagcaatc
     2581 gaatgaaata aacctaaaga tcgcaaagct gaaggagata gatatccatg tttattataa
     2641 agataaagac agtcagattg atattgctga aatgaaaaca atcactggga aaataggaga
     2701 gaagtataaa gaaactccta ttgatattga agggtatgtt tttgactcgg attcagcgaa
     2761 tacagaaggc atcttctccg aaaatacaga ggatatctat ttttattaca gaaaaggcaa
     2821 actttatctc tcaactgctc ctcaatatgt caattttgga aaacataaaa tatccagtag
     2881 accattaagt gaattcggac agttttcagg tgggttgaaa attatagatg aacgggcttt
     2941 tggaagttgg aaattacaat tgaaacaaga gaaagttctt acaaatggag attttgagct
     3001 tccagaagct ctttcctttg ttacctctga aaatacaacg gttataggga catccgctgt
     3061 tacgattttc gaaagtgatc aaaagggtga aagcgatttg tcctctcttt tagattctac
     3121 agctcatcgt ggaatcagaa tagatattcc tgtagaaaac caaagaatcg gaacctttga
     3181 ggggaaacta tcttggatac ttgcagatgt cccaggaaat taacaggaag agaagaatat
     3241 aacaaaatta ttgagtttca agaagggcac aggtaccata ggctcttgtg tcccacagag
     3301 acaagatttt gttatagtct tctcttacat atgattaaag gagcgatttt cttgagacca
     3361 aaacaacaat tatttataac actcctagtt attttagggg cactttttaa caatatatca
     3421 gtgtcagcta gtgcatttaa tttttcagtg acgccgataa cttcagagaa tcaaatcgac
     3481 aagagaaaaa cttacttcga tcttcaactt gatccggatc aggaagtaga agtaaaagcg
     3541 gaattgagaa atgatacaga aaaagaagtg aaaatcgata tttctgtgaa tagcgcaacc
     3601 accaattcaa atgtgatggt tgaatacgga aaaaacgaga ttgaaaaaga catgagcctg
     3661 atctttgatc tggtagatta tgtgagctat ccacagatag tgacgctaaa acctaaaagt
     3721 gtccaaaccg ttgcttttca tgtcaggatg ccgaatgagc gatttgatgg tgtattggct
     3781 ggtgggatca cattccaaga aatccagacg gaaaaggacc aaacagaaac taaggatcaa
     3841 agcttatcga ttgagaatga atacacctat attattgctt tgttgatgca acagacactg
     3901 aacgaagttg ctccgaacct tactcttcat gaagttaaac cagatcaaat caatgcaaga
     3961 aatgtcatct tagcaaacgt tcaaaatgat caaaaaacct atatcaatca agtagttatt
     4021 gaaacaaaaa ttacaaaaaa aggacattct gaagttttat atcaagagga aaaagaagga
     4081 ttgcagattg cgccgaatac aaatttttct tttccaactg ctttaaatgg tcaaccgcta
     4141 accccaggtg aatatcacct tacaatgact gttttaggaa atgaaaatga aaatggaaaa
     4201 ttttcgagaa aaaaaggaaa tactacgatc aactatacca atcaatgggt atttgaaaaa
     4261 gattttacaa tagacggaaa agtagccaag gaattgaaca cgacggatgt tacgattaaa
     4321 aaagacaata attggattta tatcttagta gcttcacttt tcttattgtt tattttatta
     4381 tgcatacttt tgtttgtttt gagaaaaaaa gaagagaaag aaaagtaagg ttaagataac
     4441 aaatagtaaa tttttattat tgtaggatgg tt
//
LOCUS       DACDMM010000181         4461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDMM010000181 DACDMM010000000
VERSION     DACDMM010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.184"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            574..1008
                     /locus_tag="IWD16_002471"
     CDS             574..1008
                     /locus_tag="IWD16_002471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089463.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="IWD16_002472"
     CDS             2298..3221
                     /locus_tag="IWD16_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9089464.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="IWD16_002473"
     CDS             3237..3776
                     /locus_tag="IWD16_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9089465.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DACDMM010000182         4391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDMM010000182 DACDMM010000000
VERSION     DACDMM010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.185"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            104..1063
                     /locus_tag="IWD16_002474"
     CDS             104..1063
                     /locus_tag="IWD16_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9089466.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            1180..1488
                     /locus_tag="IWD16_002475"
     CDS             1180..1488
                     /locus_tag="IWD16_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9089467.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            1515..1814
                     /locus_tag="IWD16_002476"
     CDS             1515..1814
                     /locus_tag="IWD16_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP9089468.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            1988..2539
                     /locus_tag="IWD16_002477"
     CDS             1988..2539
                     /locus_tag="IWD16_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP9089469.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            2685..2978
                     /locus_tag="IWD16_002478"
     CDS             2685..2978
                     /locus_tag="IWD16_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089470.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            2971..3267
                     /locus_tag="IWD16_002479"
     CDS             2971..3267
                     /locus_tag="IWD16_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089471.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            3338..4342
                     /locus_tag="IWD16_002480"
     CDS             3338..4342
                     /locus_tag="IWD16_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9089472.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT         1629 a          665 c          853 g         1244 t
ORIGIN      
        1 ttattaaaac atcgattgta aaattaagct agacaactaa aaagatcatt tgtgctacac
       61 tcaaaatagt tccgaccaaa gaattaataa ggagtgagta caaatgactt acacccatct
      121 tacaacagac gaactagtaa tcatagagtc ttatttcaat atcgagaaac ctgtcgctct
      181 tgtcgcacaa agtttaaatc gctctagaca aaccatttat aatgtttatt cgtttctcaa
      241 acaaggaaaa aaagtgctag attattaccg tcaatacaaa caaaataaat ctcgttgtgg
      301 acgacgttca accgttttat ctgatgaaca atcaacatat gttcaagaaa aggtagctca
      361 aggatggtca cccgatgtga ttatcggccg tggcgagttt ccaattgatt gttctgttcg
      421 tacactctat cggatgttta aaaaaggcct ttttgacaca cagtcgttac ctatgaaggg
      481 gaaacgtaaa ccgaataatc atcaagaaaa gcgtggaaaa caggcctttc gcagaacgat
      541 tcatgaacgg aacagtctct atccacacct gactaaagaa tttggtcatc tcgaagtcga
      601 caccattgtg ggagttaaac acaagagcgc tgttattaca ttggttgagt gtttatccaa
      661 ggttattatt actttgaaac cgacaggcag acgagccgaa gatatcgaaa acaggttaaa
      721 tgattggttc aaatccatgc ctaaacactt gtttaaatcc atgacatttg attgtggaaa
      781 agaattttcc aactggcgat ctataagtaa ctcgaacgat attgccatct ttttcgcaga
      841 tccaggaaca ccttcgcaaa gagggctaaa tgagcactca aacggcttgc ttagacgaga
      901 tggcttacct aaaacaatgg actttaggga cacggatgaa acctttattc aatccgttgc
      961 ctcgaaaaga aaccatattc caagaaaatc attaaattat cgaacaccat tggaagtatt
     1021 tttgagctgc atagataaag acatattgtc tagcttaatt tgacaaatca gatagtataa
     1081 aatgaatcaa attgaatatc aaatatatat tatatccatg aagcccaatc ttctttaata
     1141 gaagattggg ctatttttat ggcaaaggag ggaaaagaaa tgtttcaaaa taaaggacaa
     1201 cgttatgtca caaagggagt aatggacagt ttgccggtgg aattacaagc tttatgttgg
     1261 aatctaatcg atcaaaacgt tcaaaagcaa tttcatttag actatcttca aatctttgaa
     1321 ttttctacgg aaaaaggaaa tcaaaagttg gttcatcgtc aagaagaacc agaagcaaga
     1381 aaagagtatc tgatttcacc taagcttcgt ttgaaatcag tgtctcaaaa aatatgggtc
     1441 attgattcgg gtgagtatca aacgatgtta ttaccagaag aatactgata tctaaaagaa
     1501 aggaacgaaa ggacttgtta gaaacggcta tcgaagtact ggaaaagtgt gcacagctag
     1561 tcactactcc tgaagagtgg ggctatgaat ccgttacgat ggaaaaagag gaaattgaaa
     1621 tgggtatgct tcctgaagat gttactctac cacgtttagt gatgacacat ctttatattt
     1681 attgcgcacc agaagatgga aaagactatg tagtttattt cattacagat attaccagcc
     1741 agcgagaatt tgttcgtggg ttactggtag aaggacgatt ggtttggagt cagattggag
     1801 gaacgaatga ataagatcga ttatttgaga atgattgcga aatgtttaga agatatcctt
     1861 acagcgattc atcaaattct gaagaaaaaa ggagaaagaa tatgaaatat tttcaactag
     1921 tattgacact tataacggca atggtgattg aattgcttga acaaaaatta gcataggaga
     1981 aaaagagatg attgaaaaag aattagtgac cacacaaaca gaagtttggc gagatcctca
     2041 tcatacccat cgctatgttt tcaaacgaca atgggcgatc aaaggaaaag aagcggaaga
     2101 taaattagcg gtagtcatta ctattcgtcc aacagataca gaaccattta cgaatgattt
     2161 gagtatgtta ttaatcgaaa agaatatccg acaattagga tttactggtt ttattgctgt
     2221 gaatcttttt tcgtatacaa aagcgaaaag tcctcgggta tttcctcgtg gaaatgatga
     2281 acaatctttg gaaatattaa caactgtttt cacagaaaag aaaatcaaca ccattatttt
     2341 tgcttgtggt tcgattacag cgacgagtca agtggcttat gaacaagcga agaccattta
     2401 cgatcaattg agtgcgaaac aaaaaaagat gaccaaagta ttgataaatg cagaaggtaa
     2461 gttatcccac ccactaagta ttcatgttcg taatgaatgg caattggcag atagtcaatt
     2521 attatttcaa gaaaaataac attttatttt cttatgttac ttataagtag ttttacatgc
     2581 tttttgactt taaaaataca tgtgatgata catgttatat gaattatcaa tgtacccagt
     2641 aatatgctat tttcattatg taaaaaataa cagggggtgt gtagatggag attgatgaaa
     2701 cttgtttgag taactcttat aaaagtatga atgacttttt tgaggaaatg gaactggatt
     2761 cagaattgct ctatcaattt tatcttgctt ttcgagggca acagatttct tttccaatga
     2821 aaatgtacga tcgtgagtta gttagaaaaa gaatcgaaaa catgatagaa caggaaaaaa
     2881 ctgtagatat ccgtcaattg acagaagttt atggtttctc tgcaagatgg attcaaaaag
     2941 taattaaaca aaaagaaagg agacgagtac atggctaagc tgattgtatt aggtggttta
     3001 atttacggtg gatattggtt atataaatat atgcttgcgc atccgaatat agataaagga
     3061 aaagtaatta agacagttgg ggtaatcata gttggactca tagctctaac taagaaaaat
     3121 aatactaata gcccaaaaat aatgagaact tatcgctgta gaggatgtgg aagggaaatt
     3181 tctactagtt ttaatccatc tgaaagccct caaggaagag atcgttgtca tgctggctat
     3241 cgacattctt gggaaaggat taactaaatg aacaccattg agattaattg actgataaaa
     3301 taagtacgcg agggagaaat tgaaaggggg aaataaaatg aaaaaatggt tgttattact
     3361 aattggaatg gggattttat tcactggttg tacatctgag aatacgagga ctaaagagac
     3421 tgtagaaact gatacaacaa ttgaggctaa aaaaagtaaa acgcaatcaa ctgaacaaac
     3481 tacaacagaa atgaaacaat ctgtggaagc aagtacaagt gaaagttcaa tcattgaatc
     3541 ttcttctgaa gaggttaaaa atactttgtg ggatagtgag aaatctactc aactagcaat
     3601 ttttgtccca caatggggaa agacattagg tcaagaatat aaaagctaca atcctcagaa
     3661 caatgtaagt ctgtatggta cgcctttacc aagtgcagtt attgatggaa attggaaaat
     3721 ggcaatcaat gaagcaccag ttaccgtaca atggtcagaa gatggaactg gtcatgcaga
     3781 tttcaattta gtagcagttt attctgatgt agaaactgga gaatatttag ggcagcatgt
     3841 atatttcttt ggctttcaaa atggacaacc taaagtttat ttaacgcaac aaaatcaagg
     3901 caatgataaa aactacatgt acttcaatga aactcagaac caacagttaa aacaaggttt
     3961 cattgatatt gtgaatggac aaactcctca aactccagta gtagaagaat ctactcaaca
     4021 agcaacaact acaacgacac aatctacaag tagctcagca atttcaagtt gggaagctgc
     4081 taaagaaact gtactaaata ataaagcttt atggttgtat gattctcctg ataaaaatgc
     4141 atcaaatact aatttaatta gcacaaatga atatatgaaa gatctaatgc aagatgaaaa
     4201 tggaagctac tattcaatta atattgtaac ggatgataat ggaaatacaa caatggctgg
     4261 tggaaaacaa ttccgtgttt atacagatgg aagaatttct caacgtattg gaatgactaa
     4321 caattttcaa atgattaaat aattgaaaat attatttatc aaagaaaaac tgattttgag
     4381 aaaaattatt t
//
LOCUS       DACDMM010000183         4345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDMM010000183 DACDMM010000000
VERSION     DACDMM010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.186"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(387..1853)
                     /locus_tag="IWD16_002481"
     CDS             complement(387..1853)
                     /locus_tag="IWD16_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9089473.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IWD16_002482"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IWD16_002482"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9089474.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DACDMM010000184         4329 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDMM010000184 DACDMM010000000
VERSION     DACDMM010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.187"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            433..1344
                     /locus_tag="IWD16_002483"
     CDS             433..1344
                     /locus_tag="IWD16_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089475.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1831..2097
                     /locus_tag="IWD16_002484"
     CDS             1831..2097
                     /locus_tag="IWD16_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089476.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2169..2510
                     /locus_tag="IWD16_002485"
     CDS             2169..2510
                     /locus_tag="IWD16_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089477.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2565..2984
                     /gene="ssb"
                     /locus_tag="IWD16_002486"
     CDS             2565..2984
                     /gene="ssb"
                     /locus_tag="IWD16_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9089478.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3061..3357
                     /locus_tag="IWD16_002487"
     CDS             3061..3357
                     /locus_tag="IWD16_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089479.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3372..3803
                     /locus_tag="IWD16_002488"
     CDS             3372..3803
                     /locus_tag="IWD16_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089480.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            3985..4290
                     /locus_tag="IWD16_002489"
     CDS             3985..4290
                     /locus_tag="IWD16_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089481.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1583 a          675 c          930 g         1141 t
ORIGIN      
        1 aaaagggaaa aaatcaaccc ctaccccagt cttggggtct caaaactaac tcccccctaa
       61 ccccctcttt cagcctcttt tgaaagaggg ggagaatcaa ccccacggcc aacgccccag
      121 tgttcttggg gctgtccaga acagaaactt gcgagagagt gggggtaaaa caagtgtaga
      181 ggaaacttga agacttgttc gaacagaaac ttgcgagaga gccggagtaa aacaagtgta
      241 gaggaaactt gaagacttgt tcgaacagaa acttgcgaga gagttggagt aaaacaagtg
      301 tagaggaaac ttgaagactt gttcgaacac aaacttacaa gagaggggag taaagcagtt
      361 gcagaggaat caggaagatt tgttcgaaca gaaacttgca agaaagcatt attaaattga
      421 aggtgatgaa atatgaagta tttattgcat ggctatttta ttgattattc tactcgcaat
      481 agtagtactt tagcgattct atgtttgctt catgaattga gagtggttac tagaaaacag
      541 ctatacaaac tattgaattt tgacagatca attagtaaac aaggagtaac gaatgcttta
      601 gtgagattat atgaaaaaca gttaatcgat cgaaaaaaat atggaacaga aatgtattat
      661 tatttgacga aagagggaca tcaaagcata ggtgggtatt atacgttgcc gaaagttcct
      721 gaatataatt tgaatcacca tttacaaatt aacaattatt tgattaaagg aatggcattg
      781 gcaaaagacc acccacattt aaaacggatt atttcagaaa gacgtaccgt ctatgaaacg
      841 aaggatttaa atcctaagac aaaaggaaga aaattttttg tgccagattt tatttttcgt
      901 tttcgagaca aagaaaatca tgagatcaat tggtcctttg aaattgagct aacgttgaaa
      961 acacgtcgtc gctaccgtga agcgattttt ccgaaatata ttcgtgagtt gcgaacacgt
     1021 cctgaatcac atttaattta tgtgacacct agtggcgcaa ttcaagaaga acttcatgcg
     1081 tttaagcgta tttttttcaa gcgcgaaact gaaaaagatg cgagtcaaca agaattgttt
     1141 gagcggttgc atattttttc agctttagag tttgaagagg gattaaaagc attattagag
     1201 aatgatccag cagtcaattg gcggacagta gaacgagaaa aaatttatcc gaatggcgct
     1261 gttcaaagtc gtttgaattt atttgaagaa tcaactgaaa atgaggaaaa accaaaagtg
     1321 aagtttacgc ctgaagtatt gtaaaagagg gtgttcgctt cgctcttttt ttgtgccagt
     1381 ttgctggcac cgttcagacg caagcgtctg aaccggctcg tttttaggaa ggactaactt
     1441 gtttgattta tccgttgtca aagaagtgga cgttcgagag tccctaatcg tatagcagtc
     1501 attcggcgag tataacctaa aacataacct gccaattgcc aagggtaaaa tgcacgctgc
     1561 gcgcccttgg caatcgtcag aacatgtttt cgagccggtt atgccgaacg accgctatac
     1621 gaaaagggcc tctaaacaga agtccacttt gatccttgac aactgaaacc caaaatcaaa
     1681 caagcaggcc ttcgtctaaa aacgagccga atgaagacct tgggggttgc acccccaaac
     1741 ccccgtgccc ccgacacact accgaaaagg tgggggtggg tggcgaaaaa atcaaataaa
     1801 acattctatt caagaaagga agaaacaacc atgacaacta aaaacgagca actgaaagaa
     1861 caggcaagaa aagagttgca agaaagaggt ttgattattg aggggagctt tgaaggagat
     1921 tttgaaacct atattggttg ttatgcaaga ccgataaaca aacctacagc cttagaccca
     1981 atgaacgaac aagaagtaaa agaacaagaa aaacatgcag tgaatggttt tccgcaagat
     2041 ttcactgaat ggtatgagtg ggaaattaac aacgggaagc ttgaaaactt cttataaaag
     2101 tcagatgacg tgcaaagaga atgattctct ttgtacggta aaaatataaa gaaagaagga
     2161 aaaagaaaat ggcaaaaaac acaccttatc aagtaggaga cattttttat agtagttggg
     2221 gctacgaaca aacgaacgtg actttttggc aaattgtgaa gttaacagaa aaaacagcat
     2281 ggtttcagcc tattcaaaag aaaacggtcg agcattattc gtccatgagt ggaaaagttg
     2341 ttccaatgaa agacaagttt atagagtatc ctttagcaaa aactaaagac agtattgtaa
     2401 aaaaacgaat tgatccagaa acctatcctg atatgttatg tggttatcgt agttgggaca
     2461 cgtttcatag atataaaaat caagctgttt atgaaagtag ttattattaa ggggatagtt
     2521 tcccctttca aaaataataa atagaagaat ggagagaaaa aatcatgatg aattcagtga
     2581 atttagtagg acggttaaca aaagaagtgg atttaaaata cacaccaaaa ggcaatgcga
     2641 caggcacatt tattttagct gttaatcgaa attatacgaa tgcaaacggc gagcgtgaag
     2701 cagattatat ccgctgtgtc atttggcgga aagctgcgga aactttagca aaatatactg
     2761 gaaaaggaag tttgattggc attaatggac gtcttcaaac gaggagctat cagaatcagc
     2821 aaggacagac catgtatgtt acggaagttc tagtgacaga tttctatttg ttagagagta
     2881 aagaaatcaa tgagcaacgg tcaaaatcaa tggcgaataa tcaaaataat tcttcaattc
     2941 caggaaatga gattcaaatt tcagatagtt atttgccctt ttaagtgaaa aataattttg
     3001 ttctatacct gatttcttca ggtatagaac aaaaaataaa tagagatgag gcgatttttt
     3061 atgaaaatca ttgacagtta cgatcaacga accatgcaat atttgggctc gtttgattat
     3121 acagatgaaa atattattaa ttatgttgca agtttacctc cttatcaaag cgttcgcttg
     3181 gtagattttg caagtgatga attggtatta acaacgatgg gcaacttttt agattatgtc
     3241 ggagacaaaa aatggttgga aaaaattttg tctaaattga ttgcgaaaca aacaggagaa
     3301 gaacccataa atgcgattca attgttcaat cgatatgaac aagaaaacaa tgattaaaga
     3361 ggagagaaaa aatgaaaaga ttaaaacgaa gtatttttat tgtgctagca ttgattgatt
     3421 ttgttgggac aggttgtgaa gcaattgacg ttcaaaaagc tcccttatcg gaaataaaac
     3481 aagcaataac aaaacaagat gaagaacaac cgctaaatac acaagtagaa atagtgaatc
     3541 aaaataaacc gagtttttct gaagaagatt tttcgttaga aaatggaacc aggcaatttt
     3601 tttcagattt ggatcgttta aatcgggtag gtgtagcaag tgcacttttg cataaaagca
     3661 ggatgccaac agaagaacga gaccgattat acattaagcc aactggctgg aaacaaaaga
     3721 aaatgaagaa tggcgattat ctttataatc gttgtcattt gattggctat caacgtgcga
     3781 caagtttccc cataagtatg taatacgaaa cgggaggatt aatcttaaga ttaatcacaa
     3841 ctaacctatt tgacagttgg aatgaaagct atcatgtctg ctgaatcaac tggtctgata
     3901 ctcctacatg caataaagtc tactaggaag ttattgtatg aagctaggta aaggcggcag
     3961 aacaatttct aagtgtggaa tcatatgaag ttggatatgc cgggacgcta taaattactc
     4021 agggtgagac tgtttttaat taaactgacg aacttgcgaa tgtacgggtc tataaccgac
     4081 ttatattcga aagatataag attgcgaatg caaagaacat tatcgaaaag taagaatgtt
     4141 ggtcatgaaa ttcgagatgg aagactagcg gtgtatctgt caagagttgg taaaacatgt
     4201 ttgattgatt cagatgagag tgggaatgcg acaaaacgtt ttacagaaga aatagagcaa
     4261 ctagcagaac tgaatatacg atatttttaa atactttagc ggagaataaa aaaatcctct
     4321 acagaaaac
//
LOCUS       DACDMM010000185         4324 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDMM010000185 DACDMM010000000
VERSION     DACDMM010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.188"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..281)
                     /locus_tag="IWD16_002490"
     CDS             complement(<1..281)
                     /locus_tag="IWD16_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9089482.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..1272)
                     /locus_tag="IWD16_002491"
     CDS             complement(730..1272)
                     /locus_tag="IWD16_002491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089483.1"
                     /translation="MKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGN
                     DFVDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADL
                     QVENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTL
                     FYRQNVSQRKLERSEDEIEW"
     gene            complement(2049..2239)
                     /locus_tag="IWD16_002492"
                     /pseudo
     CDS             complement(2049..2239)
                     /locus_tag="IWD16_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349771.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2359..2721)
                     /locus_tag="IWD16_002493"
     CDS             complement(2359..2721)
                     /locus_tag="IWD16_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089484.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3033)
                     /locus_tag="IWD16_002494"
     CDS             complement(2725..3033)
                     /locus_tag="IWD16_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089485.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3042..3461)
                     /locus_tag="IWD16_002495"
     CDS             complement(3042..3461)
                     /locus_tag="IWD16_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089486.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="IWD16_002496"
     CDS             complement(3454..3687)
                     /locus_tag="IWD16_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089487.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="IWD16_002497"
     CDS             complement(3684..>4324)
                     /locus_tag="IWD16_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9089488.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DACDMM010000186         4310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDMM010000186 DACDMM010000000
VERSION     DACDMM010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.189"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(65..1285)
                     /locus_tag="IWD16_002498"
     CDS             complement(65..1285)
                     /locus_tag="IWD16_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9089489.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1910)
                     /locus_tag="IWD16_002499"
     CDS             complement(1305..1910)
                     /locus_tag="IWD16_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9089490.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="IWD16_002500"
     CDS             complement(1959..2891)
                     /locus_tag="IWD16_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9089491.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="IWD16_002501"
     CDS             complement(3312..4172)
                     /locus_tag="IWD16_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9089492.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DACDMM010000187         4203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDMM010000187 DACDMM010000000
VERSION     DACDMM010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.190"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..354)
                     /locus_tag="IWD16_002502"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="IWD16_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="IWD16_002503"
     CDS             complement(614..1162)
                     /locus_tag="IWD16_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089493.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="IWD16_002504"
     CDS             complement(1163..2020)
                     /locus_tag="IWD16_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9089494.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="IWD16_002505"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="IWD16_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="IWD16_002506"
     CDS             complement(2582..2911)
                     /locus_tag="IWD16_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9089495.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2988..3260)
                     /locus_tag="IWD16_002507"
     CDS             complement(2988..3260)
                     /locus_tag="IWD16_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9089496.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3260..3523)
                     /locus_tag="IWD16_002508"
     CDS             complement(3260..3523)
                     /locus_tag="IWD16_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9089497.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(3629..4045)
                     /locus_tag="IWD16_002509"
     CDS             complement(3629..4045)
                     /locus_tag="IWD16_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089498.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4051..>4203)
                     /locus_tag="IWD16_002510"
     CDS             complement(4051..>4203)
                     /locus_tag="IWD16_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9089499.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1225 a          851 c          533 g         1594 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaaca
     3061 acccggtgtt gtctattcaa tcttctagaa tacctacctt catgtgtagg ttttaacttc
     3121 tcaaatgatt gggtagggag atagggatct tcctttaaag tctgaataac cttctcaaat
     3181 tgaggtttca aattagactg tttgattttc tttaaatcag ctttagccga gtttttaatc
     3241 atcacagaat agttattcat tataagttat cccaatcaat ttcatcaaca ttagtaaatc
     3301 cactatcatc tttttctcgc ttacgaacaa cacccaaagt gcctatttgc tcaagtaata
     3361 gcgtttcttt gattgctttc caatcatcca aaccaataag caccgcatta ttttcactgc
     3421 gctcactaat gatttcaatt tccgtatgat tctcatttac atctttcaac aacttataaa
     3481 aatttttccg tgctgagcta ggatttaaga ctgtttttgc catatctact ccccctctaa
     3541 tttatatctc cattctaacg tacttaatta agtacgtaaa ttcgattgtt ttgtttaaat
     3601 tggagtacca aaacagcatt gtaaagtgtt atccgtttcc tttattttta ttaacaagtt
     3661 ttatcacagg cttcatatat ttgattataa cttcatcttc tacgtagata tctgttatca
     3721 ttacgacatc tgtactattt cgattgagta ctttcacttg atctccagca ttgattttcc
     3781 ctctaaatct tttaggaatg aaccaactat acatatgatt agcatcacca aaatgtgctt
     3841 ttatcgcatt ctcaggatat aacttattca atagttcatt ctttttaatt ttcaaaactt
     3901 caaatctata cttatcatca tctgctaatc taaataaatc ttctaaacgc atgttttcta
     3961 actgtaagtt ttgaaaatct tccggtataa attttcggtt ctgttgcgaa gtttgaaaat
     4021 caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt attcccatta ataccttgat
     4081 ttcagtagac accgaaaagc tgaagagcgt tccatttctt cggttctttt tatatattcc
     4141 tcgaatggtc tccatgccct taatcgtgga agaggctgta cggagacttt gataaaattt
     4201 att
//
LOCUS       DACDMM010000188         4192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDMM010000188 DACDMM010000000
VERSION     DACDMM010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.191"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(244..1515)
                     /locus_tag="IWD16_002511"
     CDS             complement(244..1515)
                     /locus_tag="IWD16_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP9089500.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            complement(1799..2746)
                     /locus_tag="IWD16_002512"
                     /pseudo
     CDS             complement(1799..2746)
                     /locus_tag="IWD16_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2758..3231
                     /locus_tag="IWD16_002513"
     CDS             2758..3231
                     /locus_tag="IWD16_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9089501.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(3404..3958)
                     /locus_tag="IWD16_002514"
     CDS             complement(3404..3958)
                     /locus_tag="IWD16_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9089502.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
BASE COUNT         1370 a          752 c          627 g         1443 t
ORIGIN      
        1 atagctcctc ttttcatatt tgaaagtgtt tactaacata taatcataca ttttgaaaat
       61 aagcaaattt cgttaaaaat aggcactgta atcataatta aaaacaagaa aaaatatccg
      121 aacaatttag agtaaataat gctaattcta gtgaatttgc gacgaggaac tgctagatca
      181 cagttcattc tctctatcgt tcggatattt tattgttaac tatacttatt tcacagagct
      241 tttttatttg attttgtgaa cagagtctcg agaaatcaat tctccctcta atagtagtac
      301 ttgatttttt atttcttctt gtttggccag tccaatcagt tggtgaaaag caatcgcccc
      361 cttgttacct ggatttagtc gtattgttgt caacgcagga tccattaatt ctgcaaaagt
      421 aatatcatca aatccaataa tcgataattg ttccatcact cgtttattct tattttttaa
      481 aaattttact aatttagccg ctaaataatc tccagtaaca acaattgcag taaattcagc
      541 taattcttga taaattcgct caaattcaga gttgttatat atccaatgcg atttaaaggt
      601 taaatgatat ttctttaatg tatcgatata agcttgatgt cgttcttgaa taacacttgg
      661 ttcacctaca atgtagtcaa aagaaagaaa cgctattttc tcatgaccat aatgaatcaa
      721 atgttccact gccaatgtac tagcttggta atcatttgta tttacaaata aacgattcat
      781 ttgttgttca agttctctta atgtctcttt tgaaagatgc gtatcaatat aaacaacagg
      841 accatcaaaa acgttattta attccaaaaa ttcttttaca cgtacaccaa caacaatgaa
      901 cccctcaaaa cgccaatttc tctgaatagt tttaacatct tctgctgaat taacattatg
      961 caccaataca aaataaccat gattctttgc ctgttcctca attccagaca aaacttctgc
     1021 ggtaaatgga tcagaaaata caaaatcagc attatcaata gccgaaaaat aaagcaatcc
     1081 tattaaaaat gcaatcgtct taccgattcc ctgacagaca gaacacattc cactaggatc
     1141 attaaatgaa taagcatttg catatcctaa actaggtgtt gcaaaacatg aaaacaacac
     1201 acgaaataac gcattgatgt tagtaatcgt ccctaacgtt gagcgcgcac tgccaccaat
     1261 cttcttttga ttgaccacaa tcgccgtaga aagattatca atctcatcaa cctctggacg
     1321 actgtattta ggtaaaaata aacgggtaaa actatcatag gtgtcattca gttgacgacc
     1381 agcttcttgg gtaatagtat caaaaacaat cgatgatttt cctgaaccag atacgctggt
     1441 aaaaatacta attttatttt tgggaatttt gagactgaca tttttaagtt gtttctcttg
     1501 cgtggataat ttcaatatac tcgttcattt ggtactcccc tttattttga aatagttgag
     1561 aatgtttcaa tgagacacgg tatttttggc agatgtttag tttacctaga aagaggtata
     1621 aaaatcaaga aaaaaagaga ggctgactca taagtagttt tattagtcga aaatgatagt
     1681 ttataaaaat aggaaccgca tcaaatcaac attctttaga atggatttga tgcgtttttc
     1741 tttatttttt gacttatgag tcaacctctt actcttcgtc atggatttga ttagctaatt
     1801 attgctccac ttgttcacga atgaaattta agtactgcgg taaaaataaa ggaatttctt
     1861 caaagcgtgg actttgattt tcatacgctt ttgcatctag gtttccttcc cctgtagcaa
     1921 tttcaatcaa taattgattt gggtcacgat aataaagtga tacaaaaaat tcacgatctt
     1981 ttattcctga atttataaaa ttgcgctctt gtaattgttt gtcgatgcgt tgtaaatcag
     2041 atttattttc tacgccaaac gcaacatgat ggacgctacc aatcccttca atggcaactg
     2101 gattctcacg atcttgaata atctgaactt cttgatagaa ctgagtgtct tgattttcta
     2161 agattgtcac tcgttgatga cccgaaaaga aactgacgta ttctttgtgc taccagccta
     2221 ccgacaataa ctctcgttca gtcgcatcgg aatattgtac gcgtagttga atggcatcaa
     2281 ttcctaaaat ggcatgctct gtagggatct ggtctgtgac atacgggaaa taatcatcaa
     2341 tatcttcaaa ttcgcgtaag ggaactaaag ctaatcgtgt tgcatctggc ccttcaaaac
     2401 gtaagatagg tcgtccagaa aatgattcga ttccataatg gcaaacgcct tgtgtttcaa
     2461 aatatatttc ccaaaaatgc aatgatgctt cggaaggaac ttttagtatc gttcgttcaa
     2521 tcgtattggt tccaaaaaac tggttgttcc cttcttgtac ttcaaaaaaa gtcagttcag
     2581 ttcccacacg gtggtgatta tctgaaaaaa acaggtgata catggtatga tcgtcttgat
     2641 taattgtttt cattagtaat tttaaaccga gtatattatg ataaaagtca aatgtcggtc
     2701 gaataaagcg attcacaata gaaatgtgat gaatacgtgc gggcataata tctaaaaatg
     2761 agaatcaaac ctataaaaga tggtgaacaa cttttgtttt catcagaaga aaataagccc
     2821 ctttatttag actatgtaaa tcacaatttg aaaatcatca ttaaagagaa taacttaaaa
     2881 catatcacac ttcatggttt tcgtcatacc cattgtagtc tgctctttga atctggtgcg
     2941 tctgtcaaag aagtgtatgt tcgacttgga catacagata ttaaaaccac tatggatgat
     3001 atatacaccc atatcgcgaa aaagcagaca gaggaaacgg caattcgttt tgcagatttc
     3061 atgagttcta cagaggaaaa gcaaggaagt ttgcagaagg tcaaaagtat ggtcaaaaac
     3121 gaaaaaagca gactatccct attagatagt ctgctaaacg ttgagttatc agtatttttc
     3181 ttattccgca gcgtttgcgt ttttgagacc gtattgattc tcttttttta atttcatcct
     3241 tttccaacct aaaaatgtta tttaattact tagtttatat aacacgaacc tataccaagt
     3301 accagtaacc ccagctttta ttccgcgaac tttatgaaat aaaatcgttc tagataaaaa
     3361 tagcttttaa tctttgtctt tactgtttat aaaaattttt aaattataca cctagtttaa
     3421 atccacgaag tgatgctcct atttcatctt cagttatacc aatgtacctt tttgtaattg
     3481 cttgcgagct atgattaaaa ataaacatta aggtagcaat gtctctagtt ttcttgtagt
     3541 agtgataccc aaaagttttt cttaatgtat gtgttcctat atcatctcgt cctaattttt
     3601 ctgctatatc tgcatatatc tgatagacac gatcccttgt tatatgtcga ccttgttgcc
     3661 gactcggaaa taaccaatca ttttcttctt taccttcagt atataataaa atttcttctc
     3721 tcaaattatc tataaaaatc ggttttcgct tacctgtttt atgttcaaca ataataggct
     3781 tacttttgcc tcgtatatct ccaactttta atcctataat atctgacata cgtaacccat
     3841 tattgattcc aaacaaaaat aaaaaactat ctctttccgg attacttgtt cttcttagcc
     3901 aaaactgaaa atcttgtatc tcttcatttg tccttaacgg ttgtacatta tagctcacta
     3961 ttgtttccct tcttaaaaat attctgagtt aatgattatg tatatatttt accataatca
     4021 ttaacttata tatataaact tttgaaagtc aataaaatca agggatttag tttatgattt
     4081 tatatcattt cagtaactca gaatattccg acttttttgt acaaaaaaat taaccttact
     4141 tatatgaaga ttctaagctc ttatttaaat tttagacggg cagttatccg ta
//
LOCUS       DACDMM010000189         4188 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDMM010000189 DACDMM010000000
VERSION     DACDMM010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.192"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..253)
                     /locus_tag="IWD16_002515"
     CDS             complement(<1..253)
                     /locus_tag="IWD16_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089503.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFL"
     gene            complement(461..1387)
                     /gene="glsA"
                     /locus_tag="IWD16_002516"
     CDS             complement(461..1387)
                     /gene="glsA"
                     /locus_tag="IWD16_002516"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP9089504.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1410..2837)
                     /locus_tag="IWD16_002517"
     CDS             complement(1410..2837)
                     /locus_tag="IWD16_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9089505.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2868..4082)
                     /locus_tag="IWD16_002518"
     CDS             complement(2868..4082)
                     /locus_tag="IWD16_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9089506.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1306 a          900 c          753 g         1229 t
ORIGIN      
        1 acagaaactg tgtgagctca gaaagagcaa taccatgttt gaatacgagt ttatacttat
       61 gcttctctct tacttgttct tttatacaat attctagaca ctcggctaga ctttctagat
      121 cgtgaaggat cgttggtgta gtcaccctta gagaaatagc tgtttgttcg atgtctattt
      181 cttggaattc aaataattgt tcacacagat acacttttct aaatatattt cgctcgatgt
      241 aattttccag cattcctgat acacgcctcc agtttttctt atgattttat ttaatcatat
      301 ctgtaaatcg tatttttgtt tcctttttat ttagaaaagc cctttcaaaa accaataaaa
      361 aaagaaactg ggacaaaagt caaacaagct cacaatttca gcctcattgt aggtagcttt
      421 gagccgtttg gacttttacc ccagcctctt tcaagaatgg ttattcaaag atgtcagcgt
      481 ctagttcttt gactacatct ttcaacaatt ggataccagc tgcgctgtta ccaaatggat
      541 ctagtgccgg gctgaagaca cctatgccgt aacgatttgg cacagctgcc atcaacccgc
      601 cgcctacacc gctttttgca ggtacaccaa catggacgga aaactctcct gattcatcat
      661 acaaaccagc agttgtcata attgatttga cgattgtagc actttcttca gtaatgattt
      721 gtttgccatt ccaaggagca attcctttat ttgccagcac agcagctaat tttgctatgc
      781 ctgtagcggt gacattgagg gagcattgtc tgaaataagc atcgagtatc tccggtacgt
      841 ctccttcgat catttgatta cctttcatat agtaagcaag agagcgattg atatcacctg
      901 ttcttgattc agaatgatag atttcttcat tcaatgtgat attaggatca tcacagatct
      961 ctttcataaa tgacaagatt cgctcaaatt tttcatctgc atctttaccg gcaatcaacg
     1021 aggttgttgc aatcgctcct gcattaacaa aaggattcat tggatgtttg cgatgattga
     1081 tttccatatt catgatagaa ttgaaggcaa aaccggttgg ttccgtgttg attgtttgaa
     1141 agactttttc tattccgttg tctttgattg ctaataacaa tacgggaact tttgacatgc
     1201 tttcgatggc aaagcgcaca tcggcgtctc ctccctcaat cagttgtttt gtatccaaat
     1261 cataaatcgc catacctaat tgtttgggat ccacttcagc taatgctgga atgtaatccg
     1321 ccacttgtcc tgtttgataa tagtggaaat tttttccaat caattgttct aattctttta
     1381 cttccatttg aataaccagc tttcattttt tatttttctg catgctggaa tttatgacgg
     1441 catgcgtaga tcgtaaatgg aataactaag acgataagat aagaaacaat cagaatcgtt
     1501 tcataaatcc catgctgtcc agctgcgata ctagcaggtg gaacaaatga gatacaaaaa
     1561 gcaaaaagtg aagtcaaaag acctaaacaa gcaacgatta cttttacaac ttttccgcct
     1621 ggaacctgat aggctctttc aagactgtca tctttaaaaa ccagcttcaa atagcccgcg
     1681 aaaaacataa agtacaacat caggtagatt acaacagtca atgacatcgc tgcaagaaat
     1741 gaaagattat tccccccgcc gcttaatgta aagacggctg cccagattgt tgcaacgatc
     1801 ccatttgcga tcatcaaatg agcaggaaca ccgtgtgtgt tttcttttgc aaaatattca
     1861 ggaagtaatc cttcttctgc tgctacgtga agtgcttttg aaggagaggt tacccaagaa
     1921 gcaatttctc cgatgacacc taaacaaaca agaatcgcaa gaattttcgc aatccatgca
     1981 agatttggat caatgtgtct taataggaat tctaacgatt gaatcacccc tgtattaaga
     2041 gacaaaccat gttgaggtac tgtagtagcg acggtcaatc cgccaaaagt atctaaaatg
     2101 attgccagaa taaccaatac aaacattgct aatggataat cctttttagg gttttccatt
     2161 tcatttgcat gactggcaga tgtttctacg cccatatatg ctaaaataaa ggaaacaaaa
     2221 acaaccagcg taggcaattt cgaaaaatca ggaatcaatg ttttcataga cagtatcgtt
     2281 tgtagtggag cgcctgaagc gacataatgg atccctaagc caaacaagac taatgctggc
     2341 aacaagatac cagctacaaa accgatccga gcaatcaatg aagtgtattt ggttcctttt
     2401 aactgtgaga gtaaaatagc ccagaaaata atcaatagga ttgccaattt cagatattta
     2461 ttgttttgta tagcaccaat acccgttgca taggataatg caccagttaa gaaatacagc
     2521 atggtcaagt agccaactgt tatctcaaac cattggaaaa agattgcggc aaaaccgaat
     2581 cgttttccca atgtgcgact gacccaagta tagacaccgc ctttttccca tcctttcacc
     2641 gttgccatct ctgctgcgca caatgccaca gggataaacc atagcaaacc gcctaataac
     2701 aagaaaaata ctaagctgaa gcctgaggtc gcgaatgtgg gatattcata taatgaaacg
     2761 accattgaga tggtcatagc caaaaagcca aagaacgtta atttttttgc aggaattttc
     2821 gttttgcttt gttgcatgaa tcttcctctt ttctatttga atttgtttca attgagtttg
     2881 ttgccaaaga agaaaaagtc caatccttct tcttgttctt cttttgtagt agtgatcgtt
     2941 gtaaagtgca tcgccgattt taaaatcact gttgtcagca cataggcata cacggcagta
     3001 atgatgacac caatcacttg aatccctaag aagtaccagc tgcttggatt cgttagtaaa
     3061 tggctgttag atgcaaagat ccctatcaga atcgtacctg tgaatccgcc catgccatga
     3121 acaccccaca catcaagtgc atcatcccat tgtttctttt tacgaaaatc aacagcagca
     3181 tgacagacga tacccgcaat gatcccaatc agcatggcac tttttgcttc tacataaccg
     3241 gcacaaggcg taatggctgc caatccagca acggatcctg ttagtacgtc gacaaaatca
     3301 aagccatttc cttttacttt tgcaatgatc agccaagtaa tcatcgcaaa tgcaagagcg
     3361 atgaatgttg aaccaaatgc attgactgct tgttcatctg ctcgaagtgc accaccggaa
     3421 ttaaaaccga accagccaaa ccaaagcagt cctgtaccga tagcagctgc cataagactg
     3481 ctatgctgac ttctttctcc taaaggaatc tttctttgac caagtgtgag gatacatgct
     3541 aaagagccga atccagctgt cgtgtggatg actgctccac cagcgaaatc tctaaatccc
     3601 atctgatcca aaaatccgcc accccatatc cagtggcaaa cagggaaata aatcagcaga
     3661 ttccaaaaaa taacaagtat aatatagcct tttaaattca atctccctgc aaaagctcct
     3721 gtcatcaaag gaacggtaat cacgataaac atcagttggt aaagaaagaa cattaaaaat
     3781 ggaatcgttg caccatgaaa catgttgggg aagcttgtaa catgtctcat caaaaagaaa
     3841 tctgccgggt ttccaataac accgccgata tcccgtccaa aggccaatcc gaatccacca
     3901 aatacccaca taatcgttgt tatcccaacg gagagaaaag attgagacat gatcgttagt
     3961 atattattct tccgtgctaa tccgccgtaa aaaaaagcta gaccaggagt catcaggcaa
     4021 accattgctg tacataaaat catgaatgct gtatctcctg tattgatagg taatgtattc
     4081 atttttttcg cctcctttct acgattacga gtataaaaga aggtgtagtt gtttttttct
     4141 tctgtttttt tgaattaata cttgcattta taaaagacag acaattaa
//
LOCUS       DACDMM010000190         4170 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDMM010000190 DACDMM010000000
VERSION     DACDMM010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.193"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            5..979
                     /locus_tag="IWD16_002519"
     CDS             5..979
                     /locus_tag="IWD16_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9089507.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            961..1422
                     /locus_tag="IWD16_002520"
     CDS             961..1422
                     /locus_tag="IWD16_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9089508.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1472..1936
                     /locus_tag="IWD16_002521"
     CDS             1472..1936
                     /locus_tag="IWD16_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9089509.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1950..2699
                     /locus_tag="IWD16_002522"
     CDS             1950..2699
                     /locus_tag="IWD16_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9089510.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2699..3529
                     /locus_tag="IWD16_002523"
     CDS             2699..3529
                     /locus_tag="IWD16_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9089511.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3529..>4170
                     /locus_tag="IWD16_002524"
     CDS             3529..>4170
                     /locus_tag="IWD16_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9089512.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFG"
BASE COUNT         1423 a          548 c          804 g         1395 t
ORIGIN      
        1 agaaatgaga caactacaca aagaacagat tgaaacagca atggcagcgt taaaggaaaa
       61 gacaattgat catgtatatt ttacagcatg tgggggttca caagccgttt taatggctgg
      121 tcaatatcta tttgataaag aattaactgt accttgtcat gtttatactg ctaacgaatt
      181 taactatgct ataccaaaag gattatctga aaattcttta gttatcactt gttcccattc
      241 tggcacaaca ccagaaactg tagaagcaac aaaaaaagca agagaatttg gagcaatgac
      301 tatttgttta tctaatgaag aaggatctcc tctatgggaa gcagctgaat atcctgttca
      361 ctatgattgg ggtaaagatg ttgatgctag tgatttaaat aaaggaattc tttatggttt
      421 attatttaat attttacttg ttttagatgg ctctaaaaaa tgggaaactt gcttgaatga
      481 atttgataag ttaaatgatc ttgttattgc tgaaaagagc aaatttgaaa taactgctaa
      541 agaatggggt aaaaaccaaa aacgagataa aattatttac acaattggta gcggtatcaa
      601 ttacggagaa atttactcca cagcagcttg ctggttcatg gaaatgcaat ggattaattc
      661 taatgctatt cattctgggg aatttttcca tggtcctttt gaaatcactg attatgatgt
      721 accattcatc ttagtgaaaa gtattggaga tacaagattt ttagatcaac gggtagaaga
      781 cttcactaaa aaattcagta ataaattaat ggttttagac caagctgatt ttgacttaga
      841 atctatagat agtgtagcga aagaatatat tgcagctgta cttagtggcg ttgttattcg
      901 ccaaatggta gaagcgattg cttttgaaag aggccattcc ttagatgtta gacgctatat
      961 gtggcaaatg aaatattaat taataggagg ttgcaaaaga tggcaaatca attatttcac
     1021 attgtgatta caacacattc taccctttgt gagggttatt taaaggcaac aaaattaatt
     1081 ttaatgaatg acgccacagg tattagtgct gttccattta aagaaaatac tagtccagat
     1141 gattttgagg agagtatgaa agaaataatt agtcagcatc atttgcaacc tctaattatt
     1201 ttaacggatt taattggagg aacacctaat aatgtttcta ttaaacagtt gaacaacaat
     1261 agaattcaaa ttattgctgg gattaattta cctcttttac tggagttatt aatggcccag
     1321 tcagctggta aatctataga agaaatagat tttaaggaat tactgaataa ctctcaaaaa
     1381 agtatggttc atattaattc gcttttaaaa gatgtcagtt aaggtggcaa tttctattct
     1441 ataaatttta aaaattgaag gaggaaatag aatgattaaa ttagtgcgtg tagaccacag
     1501 attacttcat ggacaagtaa tattttcttg gactaaacag tatgatatta accatattat
     1561 tgtggctgat aacaaagttc caggagatcc aatgtctgtg atggctttga caattgctaa
     1621 acctgctgat tgcaaattag acattgttaa aattagtgat gttaaaggga ttatggaaac
     1681 atccccaaat gataattaca tgattttaat taaaggacct aaggaagctt ttgaattagt
     1741 tcaaaattta ccagaaatta aagaaattaa ttttggcggt gtgtctaaga aaagcgagtc
     1801 caaacaatat ggaaaggcag tctttttaac tcctgaagaa ttagaagcca caaaaaaaat
     1861 tcttgatcaa gaagtgaaaa tatttgtgca acaagtccca tcttcaagta ttgaaaatgt
     1921 agattttagt aagtaagggg ggaaaataaa tgttattaaa agccatttta ttaggtttag
     1981 ttggtgtggt agctgttatt gactccaggt taattggtcg acaaaatatt ggtagaccac
     2041 taattttgag tacacttgcc ggacttgtgc taggtgactt gcatacaggg attgttcttg
     2101 gggcatcttt agaattaatt tctatgggat tcgtctcaat tggggcggct ggacctccta
     2161 atatgcaact tggtagtatt attgccactg cttttgcaat tttaactaat agtagtacag
     2221 aaacagcctt aacaatagct atcccagtgg ctgtagctgg tgaatttatg agtattatca
     2281 tgcgcatgat aattgctcaa tttggacatg ctgcagataa agccattgaa aataataaat
     2341 ttagaaaagc tcaaattatt catatttatt ggtcatttat ttttaatgct tttgtttact
     2401 ttattccaat ctttttaaca gtatattttg gtgctgacgt tgtggcagat ttagtagata
     2461 aaattccaga aatgattaca gacgccttga ctgtctctgg aaatatgctt tccgcattag
     2521 ggtttgcaat gctattaagc acaatgttga gtaaaaaatt atatccttac tttatttttg
     2581 gattctttat tgttgcctat tcaggtcttt cactaattgg cgtgacaatt tttgcagctc
     2641 tgattgcctt tgtaatggat caagttaaat atggaaaaag agaagaggcg catggttaat
     2701 ggaaaatagt acagaaaaaa aacgtttaat cacaaaaaaa gatcttaata agatgtgtct
     2761 tcgttcatgg acaatcatgt gttcgtggaa ttatgaaaga caaatgcata tgggatttat
     2821 gtatgggatg gcgccaattt taaataaagt ttataaaaat gatgaagaga aaaaaagaga
     2881 agcttatact cgtcatatgg cattttttaa ctgtacgcct caaatgactt catttattat
     2941 gggactttgt gcttctatgg aagaacaaaa tgctcaagca gaagaaggaa actttaatac
     3001 tgaatctatc tccatgatta aaaccagttt aatgggtcct tttgctggaa ttggtgattc
     3061 atttttccaa ggtactttaa gaattattac atttgggatt ggattgtctt ttgctaagca
     3121 aggtagtatt ttaggaccga ttttagctgt tggccttttt gctatacctt ctattttagc
     3181 tgttttttac ggcggtaaat taggatatga gtctggcaat aaatacctta ctaaattgta
     3241 tcaagaaggt cttatggatc gagtaatgca atttgcttct atcattggtt tggctgtagt
     3301 aggtggaatg gtggctagca tggtgccaat tgtcacccct ttaaaattca ctacaggggg
     3361 aacagaactt gttttacaag acttacttga ttccattatt cctggattat taccacttgt
     3421 atgtactttg ggaatatata aattgattaa gaaaaatgtt agtacaaata tgctcttatt
     3481 aggttgtatt ttggcagggt ttgttttaag tataataggt atcttataat gattaaggct
     3541 ctaatttttg atatggacgg agttttaatt gatagcgaga ttatgtatca aaagtgggta
     3601 tttaattttt taaaacaaga aaatttaagc tatcctttgt attcttatca aaaaaagatt
     3661 ggaactacag caagtatttt tgatgatata gaaaagtaca atgagggatg taacagttta
     3721 gaattgaagg gaaaatttac taaatattgg aaatcgcaaa agattgatta cttggatatt
     3781 tttcctaata tactaattga acagcttgaa tggttaaaaa agaaaaaaat aaaaatggca
     3841 attgcttctt ccagtccaat ggatactata aatgaagttg ttaccacttg taaaatagag
     3901 tcatattttg attgtttaat tagcggaaga gatttgccag aaagtaaacc taatcctact
     3961 atattcctta aagcttcaga acagctcaga attccagttg aagaatgtct ggtgatagaa
     4021 gattcttata atggtataaa agctggtaaa agagcaaata tgaaggttct tgcaattaaa
     4081 gataaacgtt tttctcaaga tgtaagtcta gcagatgatg ttatttacga tattaaatat
     4141 ctgcgacaag aagtacaggt gaaatttgga
//
LOCUS       DACDMM010000191         4164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDMM010000191 DACDMM010000000
VERSION     DACDMM010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.194"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..96
                     /locus_tag="IWD16_002525"
     CDS             <1..96
                     /locus_tag="IWD16_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9089513.1"
                     /translation="LKYGITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            144..614
                     /locus_tag="IWD16_002526"
     CDS             144..614
                     /locus_tag="IWD16_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP9089514.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            699..1307
                     /locus_tag="IWD16_002527"
     CDS             699..1307
                     /locus_tag="IWD16_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP9089515.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            1441..1641
                     /locus_tag="IWD16_002528"
     CDS             1441..1641
                     /locus_tag="IWD16_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9089516.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            2395..3621
                     /locus_tag="IWD16_002529"
     CDS             2395..3621
                     /locus_tag="IWD16_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP9089517.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(3729..>4164)
                     /locus_tag="IWD16_002530"
     CDS             complement(3729..>4164)
                     /locus_tag="IWD16_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9089518.1"
                     /translation="VIGIVILGFLSGLLSGNPGKYYYSLQLPPFAPPSWIFGPMWTLL
                     YILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRNIFIAAIDITLLV
                     IFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
BASE COUNT         1476 a          610 c          836 g         1242 t
ORIGIN      
        1 ttaaaatatg gtattacaca tgtgacgatc caagcagaga caaaggcaaa ggatcaaaaa
       61 aaattaattt ttttaaataa agatgaaagt tattgaaata tagagtatga taaataagaa
      121 tttttaataa ggtaggttgg ctcatgaagc agtatcgaca gttaaaagga tatttagaag
      181 caaaaggata ttgggagata ctagtctatc tcttttttgg tggtttggct acgattgtaa
      241 attttgttag ttttgctctc gcacgccaat attttgattt aagtatggcc ttatccaatt
      301 ctatttcgtg gttttgttct gtattgtttg cttttgtaac gaacaaactt tgggtattcc
      361 attcaaaatc accgaatttt acacatgcac ttattgaatg cggtaagttc ttcttctacc
      421 gtatcctttc ttacggattg gacatggggg caatggtatt gttgatcaat gttatgaaca
      481 gcaatgagta tgtggcgaaa atcatcactc aaattattgt tatccttgcg aactatattt
      541 tcagcaagtt gtttattttt aaagaaactg aggtttttga agaagaagta ggtcaaagtg
      601 aaaaagacga ctaggcataa caattgaaga atttccttct ctttgttact ctttgtaatg
      661 tagacattat taagaaaaca ttgaggagga atttttttat gacttacaca ttaccagatc
      721 taccttatgc atatgacgca ttggaacctt atattgacga agaaacgatg catctgcatc
      781 atgataagca tcacaatact tatgtgacga atttaaattc agcaattgag aaatacccag
      841 aattaggcga aaaaacaata gaagaattat tatctgatat ggacgctatt ccaacagata
      901 tcaagacagc tgtacgtaac aatggtggcg gacatgctaa ccattcattt ttctgggaaa
      961 tcatggcacc aaatgctggt ggcgaaccta caggagaaat aaaagaagcg attaatgaag
     1021 cttttggtga tttttcttct tttaaagaag aattcaaaaa agcagccgct ggacgatttg
     1081 gttctggatg ggcttggctt gtaatggaaa atggaaaatt agctattacc tctactgcaa
     1141 atcaagattc tccattgatg gaaggaaaga caccaattct aggtttggac gtttgggaac
     1201 atgcttatta tttgaaatat aaaaatgtcc gaccagatta tattgcagca ttctggaatg
     1261 ttattaattg ggatgaagta aacaaacgtt tcgaagcagc aaaataacca tttcctccgt
     1321 catatcagtc ttaaaaataa tttggaaaat aaaagagcga aagcttgcta gcgttttcta
     1381 aattgggtat aatgttcttg caaggttact tgcaagaact tttttggagg atatacattt
     1441 atggaacacg gaacggttaa atggttcaac aatgaaaaag gatttgggtt tattacagta
     1501 gacggcggag atgacgtatt tgttcatttc tcagcaattc aaggagacgg cttcaagagc
     1561 ctagaagaag gtcaagaagt agaattcacg attgttgaag gtgcacgcgg acctcaagct
     1621 gctgaagtga ctaaattata ataatttgtt gaataaagac acgacttctt agagagtcgt
     1681 gtctttttgt ctatttatga agtttttaga ttttttggct aagtaaatgc atttgttcac
     1741 ttgacatgtc ctaactaatt atcaagcaag gaacattttc ttcgttagta atggatttta
     1801 aagaaatttc agtttttgga tgttgaataa aagaccattt gtgaaaccac tagtatacat
     1861 actcattacc atgtcaattc tattacttta aaagaaaatc gttttacttt gaagaggagc
     1921 atatctatcc attaaaagat gatggtaatg caatcactag ttcgagtcat tttaagagcc
     1981 atgcagtgag ctggaaataa aacgaataga agatgggttt aacaaaagga ccagtcatag
     2041 gaagaaattg tcgtgaaatc gaatgataat ctgaacggta ggcaaagact gttaaaaaga
     2101 tgggaaaggt atgtgcgatc attttactgc acgattattt ggaaaatacc ttgttgttcg
     2161 ctttttctgg aaaaaataca taaaaaaaga taaaacgatt gcgaactgcg tgtattcggt
     2221 ctttcgatct ttttcacggt atggtatgat agttaagaat tttgagaaat tttaaaaaat
     2281 aataatcgtt acacaaaaat gttttttaaa gttttcttat gattaaaaag tgaatttgct
     2341 gattgaacag ttatatgaga caatatttta gaaaatgtga aacagaggtg tattatgaag
     2401 cgatggcaaa aagttctatt aaccttgtta ggggtattcg ttttgctgat tgccggatta
     2461 tcagcatacg gaatcagatt tatgggagaa gcgagccaaa cagtaaacaa aatttctaaa
     2521 aactctaatc gaatatcctc caaacgttct gagagagtaa gcattgatga caaagaacct
     2581 ttttcggttt tgctattagg tttagatacc ggtggattag gaagaacgga acaaggacgt
     2641 tctgatacga tgatggtagt aacagttaat ccgaaacaaa aaaaatcaac gatcatcagt
     2701 ttggaccgag atatttatac gaatatcgta ggttatggaa cggtagataa attaaatcat
     2761 gcctacgcat ttggtggtgt cgaaatggcc atggattcaa ttgagcaact gctcgatatt
     2821 cctattgatc attatgtaac gatcaatttg gatggcatgg aagatttgat caatgcggtc
     2881 ggtggcatca aagtgaataa taaaattgac tttactttgg atggtgtcca tgtaccagta
     2941 ggtgaacaaa cattgaatgg agaaaaaggt ttagcctatt ctcgaatgcg acacgaagat
     3001 ccagaaggcg atatcggtcg acaacgtcgt cagcgggaag tagtgacgaa aatcgtcaat
     3061 aaagttttaa gtttagatgg cgtaagcaat tatagaaaaa ttttaaaagc agttgagaac
     3121 aatgtgacga cggacttaga ttgggatgac atgcttgatg tagcgacgaa ctacaccccg
     3181 gcgtttgaaa caatcaaaca agaccagttg caaggcgaag gacaaatgat caatgatatc
     3241 tactatcaaa ttttaggaaa aaatgatctt ctttcgatcc aaaatgaact gaagaaacaa
     3301 ttaaatataa aaacaagcga tacattacct aatttgaaaa atgagaatgc ttcaatgatg
     3361 ttctttgatg attcggaaga tggcgattcg acaaacggtg atggatcgaa tagtgaaggg
     3421 tacaccgatt attcttccta ttcagaaaac acatatgatc agtaccaaga tccgaatacc
     3481 tattatcagc aaacagaaga tacaagtcag tactatacac caagtcaaga tactcaaagt
     3541 tatgatcctt acgctggaca ggaacaaaat ggttatggtt atgaccagaa taatgagtat
     3601 ggaacaacaa gtgggtacta gaaaaaaata gtttggttaa taaagagtgc cacctaaatt
     3661 agacaaaaaa tctaacttag gtggcacttt ttctatgtgt taaattacag gacgtctctt
     3721 tttagcgctt agtttaaaaa ataaattgaa taattcaata gagttgcgaa tagtacccat
     3781 aaataataag gaatcatgag ccacattgtc aggcgatgat gcagccacaa ctgatacata
     3841 agcactgaca agaaaataac taaaagtgta atatcgattg ctgcaatgaa aatatttctt
     3901 aaattaaaaa atagagcaga ccatataaaa ttaaaaatga actgaatcac aaaaagaccg
     3961 acaagattgt ttcgctgttt tttgttgttg tgattaagta acaaataaag agaaataccc
     4021 atcaatatat acagtaaggt ccacatcggc ccaaatatcc agctaggtgg tgcaaatggt
     4081 ggaagttgca aagaatagta atattttccg ggatttccgc taagcaaccc tgataaaaag
     4141 cctaatatga caattcctat aaca
//
LOCUS       DACDMM010000192         4150 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDMM010000192 DACDMM010000000
VERSION     DACDMM010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.195"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            102..377
                     /gene="rpsP"
                     /locus_tag="IWD16_002531"
     CDS             102..377
                     /gene="rpsP"
                     /locus_tag="IWD16_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP9089519.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            389..640
                     /locus_tag="IWD16_002532"
     CDS             389..640
                     /locus_tag="IWD16_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9089520.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            765..1556
                     /locus_tag="IWD16_002533"
     CDS             765..1556
                     /locus_tag="IWD16_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089521.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1726..2247
                     /gene="rimM"
                     /locus_tag="IWD16_002534"
     CDS             1726..2247
                     /gene="rimM"
                     /locus_tag="IWD16_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP9089522.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2237..2998
                     /gene="trmD"
                     /locus_tag="IWD16_002535"
     CDS             2237..2998
                     /gene="trmD"
                     /locus_tag="IWD16_002535"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP9089523.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3134..3481
                     /gene="rplS"
                     /locus_tag="IWD16_002536"
     CDS             3134..3481
                     /gene="rplS"
                     /locus_tag="IWD16_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP9089524.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(3613..>4150)
                     /locus_tag="IWD16_002537"
     CDS             complement(3613..>4150)
                     /locus_tag="IWD16_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9089525.1"
                     /translation="RYSLLFQWLYLTGMRPGEAIALSKDDVHITNNNASVVINGTMMY
                     RERSIADMKKSDSTKTAAGMREIDLPKKAIAIYNELLELNPNGQFLFQTTKGTPFQLT
                     AINTYLRNHKADMKIDKKLSSHIFRHTHISKLAELGTPLYAIQDRVGHENSDITEKIY
                     LHVTKGVKEKLKEDIEKL"
BASE COUNT         1389 a          743 c          835 g         1183 t
ORIGIN      
        1 aatcgaattt aatcaagctg taaagaaaaa aagctttaca gggtaaaaaa aaactgttac
       61 actacatctt gtgaataaaa caatggaggt ggaaataaat aatggcagtt aaaatccgtt
      121 taaaacgtat gggttctaaa aagagtccgt tttaccgtat cgtcgtagct gattcacgtt
      181 ctcctcgtga tggacgtttc atcgaaactg taggtactta caatcctttg aaagaccctg
      241 cagaagtagt tttaaaagaa gacttagttc ttgactggtt atcaaaaggt gcacaacctt
      301 ctgacactgt acgtaacatc ctttcaaaag aaggcgttat gcaaaaacac cacgaagcta
      361 aattctcaaa gaaataaggt gaccggatat gaaagattta agcgacttag tcttaacaat
      421 cgttcgtccg ctagtcactt atcctgatca agtcaagtta gacgtcgtag agtcgaaaga
      481 cttttacgag tacaatctaa cagtggctcc tgaagatatt ggccgtatca ttggaaaaca
      541 agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt gttcggatca atgcaccgaa
      601 aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa ataaacgtgc tgtactcgaa
      661 ttttcgagtg cagctttttt gtttttttta ttaattgaag aaatgattat ttggcaaaac
      721 aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca gccaatgaga aagaaaatgt
      781 tatttattcc atttgctcta ttgttaagcg cttgtagttc ggttactcaa gaatcagaca
      841 atacacatct atcaagcagt atatctgaaa ctgttaccag tccagaaaaa agtagtacca
      901 aaacggcttc caccactgaa caaacaatgg ctacctcaaa taatgagaaa aagactgctt
      961 tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc tctagatgta ccagtctctt
     1021 caggctatct caatatcgca gcgacacata caaagcaagg ttattctatt ttatattaca
     1081 gaacagatcg tcccctcggg ttgaatgctg acgaattgaa tcaagaaacg cctattgcaa
     1141 cctatctata tcaatatggg tttgcttcta gtcaagaaac aatccaagtt ttgcagccat
     1201 ttgagatcga cacaaatggt cagcaagtgg acttaggttc tcgaatcaca gggtatcagc
     1261 aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg aaactggcgt attcgaatcc
     1321 gtgggaataa tatagaaggc caagatccct tactattagc taaagaaatc gtagcttatt
     1381 tggaagaaaa cagtctacca gcacctgaac aatttggtaa aattactgtt gatatgggtg
     1441 atacgacgaa tcgagccgta gaagtaagct ggcaagaacc gaaaaatgcc tatacgatca
     1501 ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc tatgaagagg ttgtgaaatc
     1561 agcgcgctga tctgagccca tcatttaact ccaagtcaca ggaagtaatc aaaaatagca
     1621 cgccgcattt ttgcataatt ttggcttaat accttaaaag aaaactagaa ttgcctcttc
     1681 acacatgcta aaataaaaca gacaaaaaag aaatggggat ataagttgac tgaatacttg
     1741 aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg aagtccgtgt tatctcaaca
     1801 acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc taacactatt ccaagacgga
     1861 aaagcacctg tggaattaac agtcaaaagt catcgcaaac ataaaaattt tgacttattg
     1921 agttttgaag gacatccttc tatcaatgat gtggaaaaat atcgggatgg catattaaaa
     1981 gtgtcaaaag acaactctgt agatctagct gaaaatgaat tctattacca tgagatcatc
     2041 ggtctgcatg tattagaaga tggaactgag ttaggtaaag taaaagaaat cttgtcacca
     2101 ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag ctgatatatt gcttccatat
     2161 atcgcttctg tcgtcaaaga agtcgattta gaagctggag tggtccgtgt agaaattcca
     2221 gaaggactga ttgacgatga agattgatgt attgacttta tttccaagaa tgtttgaagg
     2281 tcctatgggg gaatccatca tcggtaaggc tgtagataaa ggattattag atatcaatat
     2341 ctctaacttc cgcgattatt cagataacaa acatcagaca gtagatgatt atccatacgg
     2401 cggtggagca ggtatgctgc tcaaagtcca gcccatctat gataacatcc aagccattga
     2461 aaaagctgca ccggatgtca aaaaaagagt aattcttctt gacccagctg gtaaacgatt
     2521 cgatcagaaa atggcagaag aattttcaca agaagaacac ttagtattca tttgtggtca
     2581 ttatgaggga tatgatgaac gtatccgcac attagtgaca gatgaagttt ccttaggaga
     2641 ttatgtcctt actgggggag aattaggtgc gatggtcatg atcgatgcaa cagttcggct
     2701 tttgccagat gtgttaggga atcagacttc agctcaaacg gattcttatt caaccggttt
     2761 attggaacat ccccaatata ctcgcccggc agaatataaa gggatgaaag taccagaagt
     2821 attgacaaat ggaaatcata aattgattga agaatggcaa ttaaaagaat cactaagaag
     2881 gacctatcaa cgtcggcctg atcttttgga aacacttgaa tggacacctc aaatgacgaa
     2941 atttttagaa gaaatcaaga aagaagaaca agaaaaaaca gcagatgaag ccaagtgact
     3001 ttttctaaaa tcttctttac agtaggttga taatatgtta gtattttatg gtgagtgcaa
     3061 aagcacttaa ctattacgat attccgctgt gagaaaagct cataagaata tttggaataa
     3121 ggagaagaaa accatgaatc cattaatcga agaattaaca aaagaacaat tacgttctga
     3181 catcccagcg ttccgccctg gtgacactgt gcgtgttcac gcaaaagttg ttgaaggtac
     3241 tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa cgccgcggtg ctggaatcag
     3301 cgaaacttat acagtacgta aagtttctaa cggagtgggc gttgaacgta cattcccatt
     3361 gcatacacca cgtgttgcaa aaatcgaagt agttcgctac ggtaaagtac gtcgtgcaaa
     3421 attgtactac ctacgtgcat tacacggaaa agctgctcgt atcaaagaaa tccgtcgtta
     3481 atcatcgtta tcagactgag aaagaaccct tggtattcaa gggttctttt tttgttctct
     3541 gtttttttat attaacgatt tctatttggg acatttttga ttaataaaaa ggggcaaaaa
     3601 aggggcaaca agttacagtt tttctatatc ttctttcaat ttttctttta ctccctttgt
     3661 cacatgcaaa taaatctttt cagtgatatc actattttcg tgcccaacgc gatcctgaat
     3721 agcatacaga ggagttccta gttccgctaa ttttgaaata tgggtatgtc taaaaatatg
     3781 tgaactaagt tttttatcaa tcttcatgtc agctttatga tttctcaaat aggtgttaat
     3841 tgctgttagt tggaaaggag ttcctttcgt tgtttgaaat agaaattgac cattcggatt
     3901 taattctaga agctcattgt aaatagctat cgcttttttt ggtaaatcaa tttctcgcat
     3961 tccagcagca gtttttgtag aatcagattt tttcatatca gctattgaac gttctctata
     4021 catcatcgtc ccatttataa ctactgatgc attattattg gtaatgtgta catcgtcttt
     4081 acttaatgcg attgcttctc caggcctcat tccagtcaaa tatagccact ggaaaaggag
     4141 agaatatctt
//
LOCUS       DACDMM010000193         4143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDMM010000193 DACDMM010000000
VERSION     DACDMM010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.196"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..639
                     /locus_tag="IWD16_002538"
     CDS             <1..639
                     /locus_tag="IWD16_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9089526.1"
                     /translation="LKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSGNI
                     FRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAVLD
                     SENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIASC
                     ITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(695..1198)
                     /locus_tag="IWD16_002539"
     CDS             complement(695..1198)
                     /locus_tag="IWD16_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9089527.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(1211..1257)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1795..2760
                     /locus_tag="IWD16_002540"
     CDS             1795..2760
                     /locus_tag="IWD16_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9089528.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(2859..4031)
                     /locus_tag="IWD16_002541"
     CDS             complement(2859..4031)
                     /locus_tag="IWD16_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9089529.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(4018..>4143)
                     /locus_tag="IWD16_002542"
     CDS             complement(4018..>4143)
                     /locus_tag="IWD16_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9089530.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         1398 a          789 c          740 g         1216 t
ORIGIN      
        1 ttaaaaacac ttgtatataa gaaaaaagat ctgacaaccg ttacagagac tagtacatta
       61 gaagaagcat taaaaatttt agaaacttcc ggataccgtt gtgtgccgat cttagattct
      121 tcaggtaata ttttccgtgg aaacatttac aaaatgcata tctatcgtca taaagcaaat
      181 ggcggtgata tgacgcttcc tgtcactcat cttttgaaaa acgctacaaa gttcatttac
      241 ctggatactt ctttcttcaa agtattcttt acgatcaaag agcttcctta tattgctgtc
      301 cttgacagtg aaaatcattt ttatggtatt ttgacacata gtacgttgct taatatgctc
      361 tctcaatctt ggagcgtaga acaaggaagc tatgtactga caattgcttc cacaggaaag
      421 caaggagatt tggctgctat tgcaaaaatc atttcaaaac acagcagcat tgccagctgt
      481 attactcttg atatcgaaaa agacgaatac attcgacgta cattgatcac gctaccggct
      541 aacactgaag cgaaagtatg tgaaacgatc gtcaaccact tagagaaaaa gaattataaa
      601 gtggtagaaa ttgaaaattt gcaaaaccaa gaagcataat caaaaagcga aaaaagaaaa
      661 cagctaatct gctttctttt ttcgcttttt ataattagac acgcttttca aaatcaagcg
      721 cgcgatctaa ataatacatc aatggtgctg aaacagccat gacgataaac tcacttaatg
      781 caagcgtgcc ataggttgtc caaaatgctt ggccacctgt tggaactaac atataagcaa
      841 tcagtcccat gctgatggta aagaagatcg tgttcaatac taaacgcatc tttacatttt
      901 tcactttgtt ttgcaataaa gctgtcaggc ctaaagaaag caatgtttgg ccgccaccaa
      961 aaaccacatc catgatacca aaaccaaaga aggcattata gacgatgacc ccgcctaaaa
     1021 ctccccaaag caatttacgg ttaaagacaa ctaaatgatt caaactttct gaaatacgaa
     1081 actggatcgg ccctgaggat acaggcgcaa ctaaaatagt taacgctaga taaagagcca
     1141 tgatgatgcc atttacaacg attacggata accgttgtct gcttttcgtt ttttgcattt
     1201 tctctcctct tttctcctag tttttttaac gcgggatggt tgatgaaccg cggttatgtg
     1261 aaacaaacaa taattataac agacttttca tttatttcaa tccgtcttat ctgcttttaa
     1321 aaaagcaaaa gcttaaacaa tgtaaacgtt tgctaaaaat cgtgggatat ggtataaagt
     1381 aagtgtaagg ggtcataaga attcccagta aagcagacga agtaggatct gcaagaacag
     1441 aattgttgtg aaacttaaat aaataaaaaa ggatgtggga atttgagcaa cctcttacta
     1501 gagaacggtc tagcaagcag ctgaagatct aagtggacat gccgaacctt ggaacttctc
     1561 cttacgtaag taatggatca tgcgcttgct aggctttttt gtgtttttcg gtcttcggac
     1621 ctttttcatt ttcctagact tctgctataa taaccaagaa ttaattttgt aacagatttt
     1681 tttgaaaaaa caagtttctt tcattcaaaa aatagaaaaa gcttgctaat tattggttaa
     1741 ccagcaaaca ggagtattaa aatatcttgt tttattgaat agcaggagga aatcatgaaa
     1801 ttccattatc atatattgat caatccatcg gcaggcagcg gaaatggcta taaagtagca
     1861 gaaagaattt taccggtatt aaaaaataag catattgact atacacttca ttatagtgaa
     1921 tacaaaggac acgatgctga gattgctgaa actttggcaa aagaaacatt gattccttgg
     1981 atagaagaag aacagaaaac aatcgatact ttcccccttt tgatcatcgt aggaggtgac
     2041 ggcaccttac atcaggtact tgatactttc tatcagatgg aagtagaatt cccagttgct
     2101 tatattccag caggttctgg agatgatttt gctcgagggg caggccttcc aaaaaatccc
     2161 aaaaaaggac ttcagctgat tttagcagct caatcaccag aaaaagtcca cataatggct
     2221 tatgaagaaa aaataagtga aaaaaaagga atagctgtta ataatttcgg tataggatta
     2281 gatgcagcta tcgtccatgc gacaaatcat tcttccacaa aaaagcgatt gaacaaatac
     2341 aacttaggat cattttcgta tttattctct attctccgtg ctttattcac tcaaaaagga
     2401 tttccaattc tggtagatgc aggaggaaaa caattaagtt tttcaaacgc atttttatgt
     2461 acagcaacga agcatccttt ttttggcgga ggaatcgcga ttgttccaac tgctgatgcg
     2521 tctaaaccag tcattgattt tgtcgtggtc gaacgaatca atttctttaa aatcttgtgg
     2581 ctcattcttt tacttataca gaaaaaacaa ttgaaatcga aatatttcca tcactacaca
     2641 acaaacagat tacgtatcgt ttcaacaaca ccgcaacatg gtcaagaaga tggagaagaa
     2701 atggaaaaac gaccttttga tatcacaata accacaacta aacagttgtt ttggtgttaa
     2761 taaaagaggt tttgtcagaa gctacatctg gaacaccgtt tattcggaaa aaaggactgt
     2821 gacacgactt ttgtcacagt ccttttttta ctatgcagtt aattctttgc ccaattgggt
     2881 tgccatcgat agtttctttc tttacccaaa ataaattgga tcattgaaaa caccgcagca
     2941 aaagcaacta agacacaacc attgaaccaa aaagattctg caaatgtttg attgtaactg
     3001 agtgtttcac tgatcaagtt attattttga acaaaccaat atgttggtgt gaatgccgct
     3061 attttgctaa ccatttctgg caagtattct gctggtacaa acaccccacc gataaagcaa
     3121 ctgcctgtga taaaaatatc tttgatccca ccgctggttt ctgagttttt taccaatgaa
     3181 gtgatacaag ccgaaaagct aatgatcggc aataaaaaca agaaagtatt taaaatatga
     3241 taccataccg ttatatccca cgaggtgtgg atcactaaca gaaccattag aagtaagaag
     3301 ccactgaaca acagcagcga gtaactaatc aaactaaagg ttactttgcg attcaagttt
     3361 cgtttggtaa tgggcgcact ctggtttcgt ttactgatct ctttgcgatt aaatgctaga
     3421 ttgatgacgc tgaaaccgct aaaaatggat agaaacaacc cataagacaa gaaattaaag
     3481 gttgtgcccg tcaacagctt tttcatccgc tgactatatc ccgcacttag ctcaacttct
     3541 ccagtaaccg agagtgccat ttgtgtctgt tccatagctg catcgattgt tttatcttct
     3601 tcttgaaaca gaatcagtgt ttgcaaaaag gtgttcactt gctgtgtaac aactgtttta
     3661 gaaaaggcat ctgggcgact ctgtatcttg atcgtcggca tctttcctac ttcgacttgt
     3721 tcggtaaagt ctgcaggcag atgtaagata atgtttacac gattaaaata aagagcatca
     3781 tcgatttctt tttgtgaggt tccatccaat gtaacgactg tttccttgtc attcaaatat
     3841 ttacgaaggc catcgactac aggcgactct tcttcactaa tcaaggcgat tttcgcatct
     3901 tgcagagagg tattttcaac atcaatattt tgcgcaaaaa agaatgtggc aacaacagtg
     3961 ataattacac ctatcagtaa attcgattta tttgctttaa gcaatgtcca aatcgtttta
     4021 taaagcgtca taacgcaccc ctctttccat ccaagcattt cctaacgcaa aaaggaaaat
     4081 aaatccgatc aaccatagaa gattggtata aaaactagcc aaatcagtgt agaaataaag
     4141 ttt
//
LOCUS       DACDMM010000194         4122 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDMM010000194 DACDMM010000000
VERSION     DACDMM010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.197"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            65..1462
                     /locus_tag="IWD16_002543"
     CDS             65..1462
                     /locus_tag="IWD16_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9089531.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            1543..1983
                     /locus_tag="IWD16_002544"
     CDS             1543..1983
                     /locus_tag="IWD16_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9089532.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            2131..2457
                     /locus_tag="IWD16_002545"
     CDS             2131..2457
                     /locus_tag="IWD16_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9089533.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            2458..3207
                     /locus_tag="IWD16_002546"
     CDS             2458..3207
                     /locus_tag="IWD16_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089534.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(3287..3742)
                     /locus_tag="IWD16_002547"
     CDS             complement(3287..3742)
                     /locus_tag="IWD16_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9089535.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         1382 a          668 c          845 g         1227 t
ORIGIN      
        1 attgatttat tttttagggt tcgttatgat tacggtatca agaaaaaaga gaggaagtaa
       61 aaaaatgaaa acctacacat tcccagaaaa tttttggtgg ggttctgctg ctagcggacc
      121 gcaaacagaa ggaagagtaa gtgatgatgg taaaggagaa aatatttggg atcattggta
      181 tgaattagag cctgaaaaat tcttcgatca agtaggacct gaaaaaactt cgcaagttta
      241 cacaaaatac aaagaagatg ttcagttaat gaaagaaact ggccataaca catttcgtac
      301 atctatccaa tggagtcatt tgattccaga agggacaggt gaaatcaatc aaaaagctgt
      361 ttctttttat cgagattatt ttactgaatt gctggataat ggtattgaac cttttgtgaa
      421 cctttaccat ttcgatatgc cactaccact gcaagaaaaa ggcggatggt tatcaagaga
      481 aacggtcgac gcttatgttt cttatgcagc agcgtgcttt gaactgtttg gagatctggt
      541 caaaaaatgg tttactcata atgaaccaat cgtacctgta gaaggaggct acttgtatca
      601 attccactat ccaaatcata tcaatatgaa agaagcggtc caagtaggct atcatgagaa
      661 tctagcaagc gcaaaggtaa tcaaagccta ccatgaaggc ggacatgatg gtgagatcgg
      721 aatcatcttg aatctgacac caagctatcc gagagatgaa acgaacccag aagatgtaaa
      781 agcggcgaag atcgctgacg cattcttcaa ccgatcattc cttgaccctg cagtgaaagg
      841 agagtttcca caagagttga tcgaaattgt caaagaattg gctataatgc cagagatcca
      901 tgaaggagac ctagaactca tccgtgaaaa cacgattgac ctgctaggaa tcaattacta
      961 tcagcctcgt cgaatcaaag caaaagaaac tccaatcaat cacgaacata gccccatgcc
     1021 agatgattat tttgataatt acgacatgcc caataaaaaa atgaatccat atcgtggatg
     1081 ggaaatttac gaaaaaggaa tctatgatat tttaacgaat acaagagaaa actatggtaa
     1141 tatcaaatgc tatatttctg aaaatgggat gggggtcgaa ggagaagaac gatttgttaa
     1201 cagcgaagga attattgaag atgattaccg gatcgagttt gttacggatc atttgaaata
     1261 cgtccatcaa gcaatccaag aaggaaccaa ttgtgtgggt taccacatgt ggacatgtat
     1321 ggacaactgg tcttggacaa atgcttataa gaatcgctat ggctttatcg ctgttgattt
     1381 ggcacaagac ggtaaacgga cgatcaaaaa atcaggtcgc tggtttaaaa cggtctctga
     1441 aaaaaacgga tttgatgcct gatatttttt gataaaacgt tcacaaaatg tttgttctaa
     1501 cactgtaatc gtgctacact aacagtgaag gaggcgttta caatggtaga tacttatcgt
     1561 aatattttag tagcaattga tggttctgaa aaggcagaaa aagcatttct tgaggcaatc
     1621 acgattgcca aacgaaatca ggcaacgttg cacatcctgt atgtcaatga agtaacaggc
     1681 aattactttg gtgattttgc atttgttaca acaagccttc aggaagaatt ggatgaggtt
     1741 gcggaaaacc agatgaaaga acatcgaaac ttggcaattg aaaaaggcct gacagatatc
     1801 gagacatatg ttctttacgg ttatccaaag acactgattg ctaatttcca tgaatcgaaa
     1861 gaaccaatcg atctgattgt catgggggcg acaggtctga atgcggtaga acgtgcacta
     1921 gtaggttcca ctacttccta tgtagtcaac cacgcatcat gtaacgtcat ggttgtaaaa
     1981 taaataaatt aagggaacag aaagcaaaaa agcataacgc ttcttttgct ttctgttcct
     2041 tttatttttt tgtaaaaaat tactttacct gatgaagtaa tcatgctatt ctaattacaa
     2101 cgacagatgg agtaatatgg aggaaagaaa atgatttcaa gtgatggaat acgcggatac
     2161 aatgatgcga ttattttatc gatccttaac aaaaacgatt cttatggata tgaaatttct
     2221 aaaacgattc gtgagaggac aaatgattcc tatgtgatca aagagacaac actttactcc
     2281 gtttttgccc gtttagaaaa gaatcaactg attgtttcgt atcctggaga agtgactcat
     2341 ggaaaaaagc ggacatatta taaaatcact gaaatagggc gtaatctttt agaagaaaaa
     2401 aagcaagagt ggctgacgac gaaagacgta gtcgatcaat ttttggaggg aaattaaatg
     2461 gatacaatcc gtaattattt agacagtctt tttatcggag tacctcaaag taccgagata
     2521 gataagctga aaactgattt gttggcaaat atggaagacc attatcatga attgatggga
     2581 gaaggaaaaa atgaacagga ggccatcggt acagtaattt cgacatttgg ttcaatcgat
     2641 gaattattag aagaactaga tgtcgaaaaa aaacatcaag ctgacgagac ggaaacaaac
     2701 acagcttcta tttatttaag cgaagcagaa aactactgga aagaatacag agcggcaagt
     2761 ctccaagtgg catcaggtgt tctattcatt agcttatctt ttgcaagttt tcttttcttt
     2821 tgttcagcag gatatgtgtt catgggtatt tcttgtctga ttttcggtat agcattagct
     2881 gtcggttttt ttattgcttc tggtatgaaa atcacacgtt taaatcactt tttgcaccat
     2941 cggaaaatac ctgaaaaagt attagcagaa gcaaaagaaa aagaagaaga gtatcagcga
     3001 agttttggct tctcactgat tgcaggaatt ggattatgta ttttttcact tttcccactt
     3061 ttggcttctt taatgtggta tatggatggt agtatcggtg cctctatatt ttttgttacg
     3121 gtaggaacgg gtgtcttctt gatcatttat ggcagtctag tccgtcattc ttatcgtcaa
     3181 ttcactcaaa gcgcttatta ctggtgaatg tattcagatt tgtaaaaata ttctatttat
     3241 ccggcgtaga aaaaaagact gtgacacgct tttgtcacag tcttttttat atataataat
     3301 ttaaaaattc atcatctaaa tccgtagaat tttctagatt gcttacccat gtaaaatcgt
     3361 tatagacaga tttcagttga cttctgcgcc attgtacagg gataaaaaga cgcatgctat
     3421 cttcattgaa tccgactaat aatttctttc cgtcagtaat gatgggacgt tgtaacaaag
     3481 taaaatgcga gacgatcaga tcgacaagct ctgaggtcgt acattggtcg atttggtctt
     3541 tcaaattttt ataatcaatc gagcgtttag ctaatagatc gtcaaatccg ttttctgtta
     3601 atgacaataa ataaaatatc tctttccttg ttaaaggctg tttaggtgtg aaaattcgtt
     3661 ctctgtaagg catttgatgg tctttcagcc agtttgccat cagatggtaa ctatgtgtgc
     3721 gagaagaata gatcgtaagc ataaaaaaac tccttaaata gtccattaat ttgttagatc
     3781 tgttgagata aataataggt aaggagctgt gcataagcat tgatcaaaac aacccaaaca
     3841 tcattagaaa taactttcta aagtcttaaa cttgcgacga gaaacaacta gactaataga
     3901 aaaaatgtct tagccatttg gcttcctgtt tgtttttttg cttatgctgc agctcctcgg
     3961 gaaagggttc taacaatttg aagaaggttg atgattgatc tagtttaaat tgtgtgaaat
     4021 ttgaaatctg gtgtttttac tagatgaatc acctaacaac tatgattata acttatagaa
     4081 cttctgtttt gcattcggtt ttgttatagt tgttataaag tt
//
LOCUS       DACDMM010000195         4110 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDMM010000195 DACDMM010000000
VERSION     DACDMM010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.198"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..737)
                     /locus_tag="IWD16_002548"
     CDS             complement(<1..737)
                     /locus_tag="IWD16_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP9089536.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEV"
     gene            complement(765..1463)
                     /locus_tag="IWD16_002549"
     CDS             complement(765..1463)
                     /locus_tag="IWD16_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP9089537.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(1475..2254)
                     /locus_tag="IWD16_002550"
     CDS             complement(1475..2254)
                     /locus_tag="IWD16_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP9089538.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(2270..3214)
                     /locus_tag="IWD16_002551"
     CDS             complement(2270..3214)
                     /locus_tag="IWD16_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9089539.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(3260..4039)
                     /locus_tag="IWD16_002552"
     CDS             complement(3260..4039)
                     /locus_tag="IWD16_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089540.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         1011 a          872 c          777 g         1450 t
ORIGIN      
        1 acctcttcat agattggata attgatctca tccccgttga tcagatcctt tgcgacttgt
       61 ttcatctgta ctactttttc cttaccaaac tccttggcaa tctgctcata gcactctttt
      121 aagtaaaagg tgcgcttaga gaccccgtgt gcatcagaag cagccatctg cacgagatta
      181 tgtttgacca ttgttttggc tgttttctgg atatctttgc caaatacacc aacataactt
      241 ggcgcagtaa gttgggccaa acatcccata tctaggaacg gaagcaaatg atttgggtct
      301 tctcgaaaat gcgcgttgcg ttctggatgg acgatcactg gtatctttcc tagttctaac
      361 aactcaaaaa aaagccgttc tgcatataac ggcacttcca gtgtcggaaa ttcgatcaga
      421 agatatgtat catctaaatc tgtaaataaa atttcatctc ggtggatgtc ttcgatgagc
      481 gttccggtaa tacgtacttc ttgtccttcc aataacgtca aaggcaactg tcttttctct
      541 aattcggctt gtaacgaagc gactagagaa atgacttgtg atttttcatt gctgtatttt
      601 ccattattgt ggtgtggtgt gcataaaata tgcgtaatgc cttgctgaat cgctttttca
      661 gccattgcaa tacttgcttc taagttttct gccccgtcat cgattcctgg taggatatgg
      721 caatgtagat cgatcatttc tcttgtctcc tctcttctta ttccttaatt tccgtagtaa
      781 taaccagcat ctttgctgtg ttctgctccg ttgtatacga cacctaagac tcgtgcttgt
      841 accatatcta acagttcttt tgctttagtc aatgattctt ttcgtgacgt attttcccgt
      901 acgaccaata aggtgccatc cgtttttgaa gacatgatct gtgcatctgt tacggcaacg
      961 actggcggca tgtcgaagat cactacatca aatagttgtc ttgcttctgc taaaatctga
     1021 tccattcttg gcgaacttag tagttctgat ggatttggtg gtttaggtcc acttggcaga
     1081 atcgacaaat tgtcgactgg tgttcgttgg atctcatctg ctacacttcc tgatgaacta
     1141 agaacagtac ttaatccgct tgcgttgttc aacttgaacg ttttatagat gacgggctta
     1201 cgtaagtctg catcgactag cagcacccgt tgaccagatt tggcaaaaac aacggcaatg
     1261 tttgctgcag tggtcgattt cccttctcca ggtcctgatg aagtcacaac aatcgttttg
     1321 atttgctgac cagctgcgga ggcaaactgg atattcgtcc gaatcgtacg atattgttca
     1381 gcgattggtg aagagggatt ggtgagtgtg accaaactga ctgcatgagt ttgggctgct
     1441 ttttgtttct gtgttcgtgc cattttcttt cctcctagac tcttgagcgg cttctgcgtg
     1501 atggcgctgt tttatcgttt gtagcttttg gttgtgtctg agctggtcct tttttacgga
     1561 taccagcatt caattctttc gctgacattt ctggaactgt tcctaagatt gggaatccta
     1621 atgtatctgt cacatatttt tcatctttca ctgtccgatc gagtaattcc aataagaatg
     1681 ccagacctac acctaccatc attcctaaca ttaagccagt cattaaattc aatttattat
     1741 ttggcgaaac aggtgtcgta ttggcaactg cacctgaaat gatcgagatt ttatcaacat
     1801 tcatcacatc ttttgcgttc tcttggaaaa cttgtgaagt tgtgttagcg atctgttgag
     1861 cagccactgc attcgtactt attgcctgaa tcgagaacat ttgtgaattt tctgcctgat
     1921 taactgagat ggcatctttg atttgttcag cagtcatttt cacattgtct tctgtttcta
     1981 aacgatcttt gacttcattg agcactaaat cactgacgat catatcttta tacgtattga
     2041 tcatttgcag gttcgtattg acatcattga catttgcagt gtctgattgt ggcagtgtca
     2101 cgatcaattg tgcttgtgaa ctgtattttg gtgtcaagat gaaaaatgtt acgattcctg
     2161 agatggccaa gccagcaaac attgagatga tgattattac caagtgtttg cgtaatgttt
     2221 caaaaatttc tttcaagctg attgtctctt ccattttttc tccactttcc tatttattct
     2281 ctagattcat ttgatctttt aattcttttt tcacacgatc aagttcttga tcactgactt
     2341 tttgatagga gatattatct tgcatgaagc cttctccttg cagctgatct tgcttaatcg
     2401 taccaaatgc ctcccgataa tctaaagcaa tttttttcat ttcatcaaag ttcatgtctg
     2461 ttttcatatt cgactctaga gcattcaaaa cggattgata gttgctgaca ccgtttagac
     2521 tcaatacttt tttcgctacg ccttctacga tctttctttg gcgcccttgt ctaccgtaat
     2581 caccatttgg gtcttcataa cgcatacgag aataagataa tgcctgatcc ccgttcaatg
     2641 taattttacc gattggaaag tcatagccat cttgtgagaa agtgaggtca ttattgactt
     2701 cgataccacc aactgcatca acgagttcct ttagcccttt catattgata gagacatagt
     2761 gatcgattgg gatgtctaca tatttttgta cggtatccat cgccatggca gctccgccga
     2821 atgcgtaagc gtggttgatc ttgtcttttg tgtcacgacc tacgatgtcg acatacgtat
     2881 cacgaggaat gctgacgatc gttgtctgtt tgtcttcagg actgactgtg gcaaccatca
     2941 tcgtatctga tcttccttga tcgacccgac ctaaatctcc agtatcaatc cccatcaata
     3001 gaacagagaa agattcttgt ttgtccagat caactggttt cgttcgttct ccttgggttc
     3061 gttccactga ttcgtaagtt ttctcaatag aatgtgagag atccagatat aattttgctc
     3121 ctactcctac aattgctaat actaatacga taagaactgc taaaataatc agaaatattt
     3181 ttttccctct tgattttctt cttcgtcttg ccatagtttc ctccgtttgc taaccttgct
     3241 tgaatcaacg ttttaactgt caatagctta tattgctata gcccatttgc tcagcaacag
     3301 tttgtgcttt tgttgcgtat gctgcgctta ttttttcatt ccggatctgc tctatttgac
     3361 tgagcaccgt caaatattga tcgtaagtag cggtggcagt cacttgacca tcctttaaca
     3421 tcgtatcgaa taactgatct agttgcgtga cttgcgcgat ctgaatatca gcgaatccta
     3481 gatattgcat aacaaccgtt ttccaaggac tatctttaaa aaagctcata ttttcacgta
     3541 cacgagcgac atctgcactt gtcacctttt ctttgatgat gacatcgtct acagcttgct
     3601 gccagtttga tacattttta gtaaataaat cgttgacagc tgtctggatc ttcagttttg
     3661 attctgcact atctaactgt gtcacgattg ctttcttctc atcttggatt gcaacagctt
     3721 ctttaggcaa ttctgattct ttgatgtcgt aatcatctgc tgaaagtttg acagaggata
     3781 gcttatttga caaacttgtg attgtttctt ctgttatttc agggtttaga aaaatttttt
     3841 cttttgtact atacagcttg tctacttctt ttgataaaga agtcaaggtg tctttttgat
     3901 tggtgatggc agattctgca taattgatcc gttgctgatg ttgccgctga ttattgatat
     3961 ggtatccaaa caaacagatc gcaatagcac ctgcacctac cgccacagca gtcaatgact
     4021 tgacgaatgg cttttccata gattcactcc taagtattga tgatagataa attaagatat
     4081 aattcactta cataaaaatg acagcttttt
//
LOCUS       DACDMM010000196         4100 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDMM010000196 DACDMM010000000
VERSION     DACDMM010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.199"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            16..1413
                     /locus_tag="IWD16_002553"
     CDS             16..1413
                     /locus_tag="IWD16_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9089541.1"
                     /translation="MGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAIFSWVLVGTA
                     MLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLGNVAFATLLM
                     SALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTFCKLVPLFLF
                     IVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVGIEGASVVGS
                     RAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMGYVFEMMVGK
                     WGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNGAPTYAIVLT
                     TLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKEWVQFLVGLL
                     ASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDKILLIGVTMV
                     GVIAMVLFATGMLHV"
     gene            complement(1594..2367)
                     /locus_tag="IWD16_002554"
     CDS             complement(1594..2367)
                     /locus_tag="IWD16_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9089542.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2364..3269)
                     /locus_tag="IWD16_002555"
     CDS             complement(2364..3269)
                     /locus_tag="IWD16_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9089543.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3423..3743)
                     /locus_tag="IWD16_002556"
     CDS             complement(3423..3743)
                     /locus_tag="IWD16_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9089544.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1241 a          757 c          812 g         1290 t
ORIGIN      
        1 caacagaaaa aaggaattgg tttaattcca cttgctgcct tagtaattgg ttcagcgatt
       61 ggaggtggcg tatttggtat tatgacggac atctccggat cggctggcgg cccagctatt
      121 ttcagttggg tactcgtagg aacagcaatg ttgatgctct ccttgtcgat caataatatc
      181 aataaaaaac gtccagattt aaaaggcggg atctttagtt acgcagaaga aggttttggc
      241 cgtttcgctg gttttatcag cggttggggt tactggctca catgctggtt aggcaacgta
      301 gcttttgcaa cattgttgat gagcgcatta ggatatttct tcccagtatt tgaaggtggg
      361 caaaatctgg catcgatcgt gatgagtaca gtctttttat ggggatatgc atggctagtg
      421 aatcggggag tcgaaaatgc tagtttcgtc aatgctatcg tcacattctg taaattagtg
      481 ccattatttt tatttattgt agtagctatc agcacgtttt cgatccaaca attcattgat
      541 aacttttcta gcgacattat attgaatgcg aatggacagc cgatcccgtt ttccagtcaa
      601 gtattgagtt gcatcatggt gatgctttgg gtatttgttg gtattgaagg agcctctgtc
      661 gtaggttcac gagcaaaaaa acaatcagat gttgggaaag caacgattct agggatattc
      721 ggcttgatta ttatttacgt cttggtttcc atgcttccat atgggacaat gacgatgaat
      781 cagctgcaaa cgatcaaggc gcagcctgct atgggctatg tgtttgaaat gatggttggt
      841 aaatggggag cagtcgtgat caacggcggt ttgatcattt ctttagttgg tgtttggctc
      901 tcatggacga tcttaccgat tgaaacaatg ggaaacatgg ctgaagacgg acttttgcct
      961 aaagcatggg gaaaactcaa caaaaatggg gcaccaactt atgctatcgt attgacgacg
     1021 ttatgtacga atgtcttttt actttcatta ttattcactg acagtgctta taactttgct
     1081 tacacacttg gtacagcagc tattttcttc acgtggcttt tcttaggatt gtaccaaatg
     1141 aaactttctt atcagcggaa agaatgggtc caatttttag tagggctttt agcaagtgcc
     1201 tttcaagtat gggctatgct agtcgtagcc tttaatgaag taatgcttgt tcttgttctt
     1261 ttcttgccag gcatttattt ttacctcaaa gcacgtaagg agcaaaacga gcctgtacaa
     1321 catatccact tagataagat tttacttata ggtgtgacga tggtcggagt gattgcaatg
     1381 gttttatttg ccactgggat gcttcacgta tagaagtata aaagaagtat aaagagaggt
     1441 tgtgagcctg tcgtttattc aaaaacatac taggaaagga gctgtgacac aaatgttgtc
     1501 acagctcctt tttcagaata aacggtgttt aagagacagc caatcggcaa taaggctgaa
     1561 cgtctctttc acagcctctt ttttgatgag tcatcataca ttttttcgct taaatacgag
     1621 atatcctaat gctaagaaca aaacgatgta agctaaatta ccgaatagca tttgattagt
     1681 agaaagatga gtcaattgct tcatgatctc ttcgtttcca atttggtttt gcagattcaa
     1741 catactgatc ggattccatt tgatccactc ccatttttgg atgagtgcaa taaggatacc
     1801 agatatgatt gacgaagcaa aataaaagac gattcctgct gagatggatg cgccggaact
     1861 attgataaag caagcaatca tcaacactaa acttaaaatc agccataaac cgatataact
     1921 acccaaagta taagtgaaca agctttggat cagtgtttga ccagtagaaa ccttctccgt
     1981 gaaggaaatc gaagggaaaa gaatcagttt catcaacaca gtgacaataa tagctaaaag
     2041 gtataaataa acagaataaa taactaatgt gatccatttg cttactaata tttctcctcg
     2101 agaatacttt cgataaagga gatttttcaa tgtaccatat tgtgcttcca tcgaaatgat
     2161 cgtgcttgca gctgcaatca tgataaagac aatccaagat gtggcactaa aaagctgagc
     2221 agtcatttca actggatcaa tgatcgtcgt gtattttttg gctagaagtg ctgttcctat
     2281 gagcaaaaca actaatagaa cagataaaac agcagttgac tttttcttga tcaatttaaa
     2341 taattcttgt tttacaagca atgtcatttt tcttcttccc cctttactga atccagaatt
     2401 tccaatagag aagattccaa atcattcgtc agatgctgga cgtctgtcac atggatattt
     2461 tgtgcagaca atagctggat cacggttcct aataattctt cgttttcttt taaaagcttg
     2521 attttatcag gatcttccaa agtatatcca gcttctgtca agatcgttcg agcagctaga
     2581 tcatttgttg tagagataac gatgaaatct tgcgctgttt ggttcaattc agccattgta
     2641 cattgtttga tgatctttcc ttgattgatc aggatcagct catcgaccat tttttcaagc
     2701 tcgctgagta agtgactgga gatcaagaaa gaggtgccct gagtagccaa gtcggtaatg
     2761 atttttcgca catctcttgt tgcttgtgga tccaaaccgt tcatcggctc atctaaaata
     2821 acgatagacg gatggttcag taaggcaaga gcaattccca atttttgttt cattcccaac
     2881 gagtaggatt tcgctttttt gtgaatgtac ttttccatct tgagctgatt cacgatagtg
     2941 tcgatagcag atacatcgtt ggtttcagag aataacttca gatgatcata gcctgttaag
     3001 aatggataga tacccgggta ttcgatcaat gctcctactt gttccaaccc tttatgacta
     3061 gagggagaaa tctcattttc accaatacag attgttccat ctgagtaatg gatcagtcct
     3121 aaaatagctt tcatgatcgt tgtttttcca gcgccattag gacctactaa tccaacgatt
     3181 ttgccagggg ataagctaaa actgacatcg ctgagggctt gtttgtgacc aaagttcttg
     3241 gataattctt ggaccgaaag acttttcaaa aatattcact ccttttctat agatagtaca
     3301 agcatacacc tgatgagtgg agaagtcgct aggtcattgg acttatttct tattttttca
     3361 taaaaaaatt gaatattaaa taaaaaggct tattttttct gatcagtact tattccaagt
     3421 gtctagtggc cgctagtaat cttgatgcca atgatactga tgagtagcat ccccacaaag
     3481 aaccaagtgg aaagattcag cttatcatga aacaagacca caccaattat gaccgaacca
     3541 actgcgccaa tccctgtcca gataggataa gagagactca tcggcaaact tttgattgcc
     3601 tttgacaaaa agtaaaaact agcaatcatg ccaatcactg tgtaaaaaga ataattgatt
     3661 ttagaaaaac catcactcca tttcatcatc gttgaccaga atacttccaa aattcctgct
     3721 atgattaatt ccatccatgc catacttaca ttcctcctta actataaaaa aatacacaaa
     3781 aagaggaaaa tcatcccttc aaagacctaa tggaatgatc atcctcttta tcctacccgg
     3841 tggcagattg tatatctttt tctgtagtat agcacaccta tccagatatc ggcaatctca
     3901 tactttttag taaagtgtac caagcactga cggatatatc gttagcggaa aagaagatga
     3961 atgctttgca atttcataga aagaagttgt tatttattaa tcatttaaaa aaaacagtaa
     4021 ctagacctta tttctatata ttatcgggca ataaattaaa aaaagcttta ccttacaata
     4081 agtaagataa tttttgtgat
//
LOCUS       DACDMM010000197         4024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDMM010000197 DACDMM010000000
VERSION     DACDMM010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.200"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            114..881
                     /locus_tag="IWD16_002557"
     CDS             114..881
                     /locus_tag="IWD16_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9089545.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            886..1865
                     /locus_tag="IWD16_002558"
                     /pseudo
     CDS             886..1865
                     /locus_tag="IWD16_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2016..2180)
                     /locus_tag="IWD16_002559"
     CDS             complement(2016..2180)
                     /locus_tag="IWD16_002559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089546.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            complement(2295..2705)
                     /locus_tag="IWD16_002560"
                     /pseudo
     CDS             complement(2295..2705)
                     /locus_tag="IWD16_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            complement(2718..3074)
                     /locus_tag="IWD16_002561"
                     /pseudo
     CDS             complement(2718..3074)
                     /locus_tag="IWD16_002561"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3385..3840)
                     /locus_tag="IWD16_002562"
     CDS             complement(3385..3840)
                     /locus_tag="IWD16_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9089547.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
BASE COUNT         1302 a          729 c          681 g         1312 t
ORIGIN      
        1 atttgttttt gtttgaaatt gtttgtttta ccttgaaaat attgtctttt atgatacaat
       61 taaaagagaa ttatctttgg aaaaaaatta ctttatgaaa gaagggtctt catatgaaca
      121 aaaaacgacg attagaaaaa attttagata tgttaaagat tgatgggacc ataaccataa
      181 aagaaataat agatgaacta gatatttccg atatgacagc ccgtagagac cttgatgctc
      241 tagaagctga tggactttta acacgtactc atggtggtgc acaattgctt tcctctaaaa
      301 agccacttga aaagacacat atcgagaaga aaagtctaaa tacaaaagaa aaaattgaca
      361 ttgctaaaaa agcctgctct ttaatcaaag atggcgatac tatttttatt ggacctggaa
      421 ctacacttga acaactggca ttagaattga aaggtcgtaa aggttataaa attcgtgtca
      481 ttacaaatag tctccctgtg ttcttgattc taaatgatag cgaaaccatt gatttattgc
      541 ttcttggcgg tgaatataga gaaataactg gagcttttgt aggttcaatg gcttcgacaa
      601 atttaaaagc aattagattt gccaaagctt ttgttagtgc aaatgctgtt acccataatt
      661 ctattgctac atatagtgac aaggaaggtg tgattcaaca acttgcccta aacaatgctg
      721 tagaaaaatt cttattagta gacagtacta aattcgatcg atacgatttc tttaacttct
      781 ataatctaga tcaactcgat accatcatta cagataacca gattagccct caacacttag
      841 aggaatttag ccagtacact actattttaa aagcggacta gaattatgac ttataaaaat
      901 attgtttttg accttgatga cactctttat gaccatcaac tcccctttaa acgtagcgtt
      961 gagaaatgtt ttccaacgat tgatatccaa caaatagata acatttacaa acgtttcaga
     1021 tattggtctg atgttgcttt tcctaaatac acgaaaaaac tcatcagtat tgagcagctt
     1081 agaatttttc gctgtcagaa gactatggaa gaatttggca tcgatagtat ttctaggaca
     1141 gaagctctag acttccaagc tgactatgaa tatgaactcg atcagattac gatgattcct
     1201 gagatacatc aactactgct ggctctacat aaaaaccgca tcccaatagg tattctaact
     1261 aatggccctg ttgacttaca gagtcgaaac tccaaaatat tggtgcctat caatattttg
     1321 aaaagcaaaa tatcatcatt agtcagtcca ctcacggagg agctgcagaa aatagctatg
     1381 aaggtaatgt aaaaaactta caaagttacc gaaatgtcca attatctaaa agggctatag
     1441 aaattcttaa agaggaaatt gagttaaatc accaacatat gagattcaat cctgattata
     1501 aagataatgg atggattttt acttccaaaa gtcgtcataa acccgactat aatggaacac
     1561 ctcttcatta tagtgtcctt aacaatttcc tcaactcacc tgaaaatgga aaattaaatc
     1621 gaaaaggtaa tccaaaacga gcaggaattg atattgataa taaacttagt tttaataaac
     1681 atattacgac ccatatattc cgtcatacgc atattagttt cttagcagag taaggaatac
     1741 ccctagaagc aattcaagac cgtgtagggc attctagagg tagtcgtgta acagaaattt
     1801 atttgcacat cactaaaaaa acaaagtatc aaattatacc tatccttgat actcttacac
     1861 aataagtgtg gcaaatctgt ggcaaaacat aagaaaaagg cttgtccata ttggacaaac
     1921 cctttatttt caagcttata taacctaatt aagctttacc agctgattag aataggatgt
     1981 tgcatgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat ttgggtgctg
     2041 ttgtttgaac gtatcattat caatgataac aacatttcaa gatcaagtcc taaaaaaccg
     2101 ctatatttgc caaaattcgc ttttaaataa gcaaattcgt tctctgtaat aaatggtgta
     2161 tctgttcttg gataactcaa taattttgct tcatacagtc cttgcatagc ttttaaagtt
     2221 tggctcgctg tcgctttata aagctgattg acttttgatt gcaaactact taaagaaaac
     2281 gaactcggac tattcgtttt cttctctttg gtttgaacat cagcaattat cccctcttga
     2341 ttgcctattt tagcttgttt agaagaaaca aaagctaaaa agctcctctt gggttttaaa
     2401 acgctgtgtg gggcttagaa cgcccttaaa tgacccttgt tttacttagc ttccacctcg
     2461 aaaaaaggtt cttttttaaa attctctatg gcttcctggc gctgaaaaat aaggtataag
     2521 gtgggcgttt gaacacgtcc tagtgaaaat gtaccttgta cgcccttctg ttgtaaattt
     2581 aacgtataca aagggcttgc gttcatgccg atcaaccaat cggcaatttg gcgtgtttgc
     2641 gcttcttgat aaaagggata gtaattcatt cctggttgca aattttgaaa accgcttcgg
     2701 atcacatctt tttctaagct attgatccat agtcttttaa atgttttatc ttttgaaaag
     2761 gcatttgcat tatggataat cgaccaggcg atattttcac cttctctgtc gctatctgtt
     2821 gcaacaataa ttgtatttgc ctttttgaga agttctgcaa caattttaaa ctgctttccc
     2881 ttatcttttg caacttcaaa atcgtatcga tcaggaaaaa tcggcaaaga ttcaagtttc
     2941 caattttgcc acttttcgtc ataatgacct ggttctgcta attccactaa atgcccaaaa
     3001 ccaaaggtga taaacgtttc atctgtaaat agtgggtcta gcaaataact agctctaaat
     3061 ataaaaaacg cgatgaataa tcataaatgt gattgcttcg tcgcgttttt tatttttggc
     3121 tgctttgtgt tgaaaagctt ccgcttttct tctagcctca agggataatt tggttagatt
     3181 catgctcata agcatcattc caatgtcagt aggtgcaacg ttcttgcctc tgacatgggt
     3241 tcttcacata ccaaatacac ctttcaatcg atcaaaaata gtttctacgt caattttgca
     3301 ttgagcgtag agggcttttc cataatcact ttcaaggcta ttatttaaaa tcgctaaggc
     3361 gtgaaggcaa atgctgttca tatctcaaaa cggttttctg actcgcaatt ttttctcttt
     3421 gtcttccaag ttgattccta agagatcacc aacatgcgcc atcaccttat gaaactgctt
     3481 ctcagtgata tgaagatcat aatgggctgt cgagggaaat aaccactcag aattgatatt
     3541 ttcttgggcc agccaatcat gatattgcaa caagtcttgc tgaacaggct ttaaatataa
     3601 cgtgtttgct ttaccggtct ttttatcatg aataaaggtt gcatttttga cagagccgtc
     3661 tggattatag acatctgatt tttttaatgt catgacatca ctcacacgta gtagcgtggc
     3721 cttaccaact tgaaaaacgg tgtaatttcg gcgaccagca cggaaactat cgagaagtgt
     3781 atcttgtacc atctttaaga cgctggagtc tttgatgggt aggacaattt gttgcaccat
     3841 gattcatgta tacctttctg aaattaaata attaaataaa taattaatta aattgtttat
     3901 ttattgatga ccaggaccgt atttctaaac ttcttttatg gtttattata tcacttaaca
     3961 aaaaaacagc ccccattatc tagtcactag ataatggggg ctgttttaat cagttttcaa
     4021 aggg
//
LOCUS       DACDMM010000198         4011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDMM010000198 DACDMM010000000
VERSION     DACDMM010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.201"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            838..1050
                     /locus_tag="IWD16_002563"
     CDS             838..1050
                     /locus_tag="IWD16_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089548.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1328..3127)
                     /locus_tag="IWD16_002564"
     CDS             complement(1328..3127)
                     /locus_tag="IWD16_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9089549.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3251..3847)
                     /locus_tag="IWD16_002565"
     CDS             complement(3251..3847)
                     /locus_tag="IWD16_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9089550.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1441 a          711 c          688 g         1171 t
ORIGIN      
        1 cagataataa cggaatatta tcatcgaaac agctaacaaa attgtcataa aaaacgagat
       61 ggttgatatt agaacttgtg tttttgttta ggtctagaga taaagagaga caatttccaa
      121 atatatgtaa tgaatacaaa aagacgtttg tcgcggatta ccgttacaaa cgtcttttat
      181 tgattttccg agatctcatc agtcgccatt tggaatggtt cactccttat ctagattcgt
      241 ttaatgctcg gactgcttgt tcgattgtaa taccaaacgc aacgttattt tcgttttgtg
      301 catcaatcgc cataaaacgg ttagaagttg tatcaaaagc tacgcggtat tgcatatttg
      361 tttttgtaaa cgtcataaaa ttcatctcct ttgtaatgta atttagttct tgaaagtttc
      421 ctttaaactt tcgaacttat accaagttta gcatcgtacg ttttttgatg caattaaaaa
      481 gcatttatca ttttccttat ttattttttc ttaaatggtt tagctatttt cttttaatcc
      541 gataagtagc ccaatcatca aggttacaaa tactattgct tgaatataaa tcatatgctc
      601 acctctttat aaataactta gcaatataaa gaaaaaaacg cacgaacaac ataggaatat
      661 aaacaaacgt ttttgtcaca acgtctaaga tcgtgttaaa aaatagttat ctaaaaagca
      721 gaaagatact caaaataaaa aataaaaaaa tggataaaaa cgttttcaaa tacaaggtaa
      781 tttgttatta taaaataaag aaaaaacaaa ataaaaataa agaaaagagg gaatgaaatg
      841 gtaaaagcag aagtaggaag atcttatata tgcaaaccga tcggagtatc tcatgaacta
      901 atcggctgta ttgaacgtac atatatcaac acggctgtcg tgtatgttga aagttatgac
      961 caacgagact atcaacttat caatgaaagc aagtatcgca tgctcgtaaa attctgtgat
     1021 attagcgcac ctgctgaatt ggtttcttaa acagtatttt tgagaattcg caagaactcc
     1081 cttataatat aaataaagac agaagggagc gagaagatat gccaaaaaga aaaagttcag
     1141 ggaaaatggg aattattctt gcagtgatct ctgcaattcc tgctatttta gctattgttc
     1201 gtgcgatgaa ggaatcaaga gaaagttaag cacacattat gagcgtcaaa caaaaaacgc
     1261 atatacctgc ggtggcaggt tatgcgtttt ttgttgtata aagacttttt tcattaacga
     1321 tttttgatta gccgactccc aaaacaaaat ttagcagttt atctgaataa gtgatttctt
     1381 catctgtttg tttcacactt tcatttagta gtcccatctc atcggttatg ataccatcca
     1441 caccataaaa catcatgcga ctcatagcat ctgaatcgtt gattgtccat acatatactt
     1501 cttttccgtc ttggtgggca gaattaatga agttacggtt gatcgtacta tattccattg
     1561 tcagaaaatc agcaggcgtc acagggggcc caacaatatt gaaaggtaag atgtagccca
     1621 cataaaagtt tggttctgct tcttttaaat ctgaaacggt ttggtacgtc aaactttgca
     1681 aaatatgatg gttctctaag ataactttgc ggtatttatg gacaaatcgt tcgacaaggt
     1741 catccgaatc ttttttagag gtcttgatct caataagtaa tttttggttt agcttatgtg
     1801 ctttttccaa ataagaatca aaagaacaaa caggggcttg cactccgttt tcagtaactg
     1861 taagatttgt caattctttc aatgtaagat cctgtggtgt tccatttacc cctgttaagt
     1921 gacgaagatt tgcatcatgc atcactacga aatgaccatc ttttgtttcc tgaacatcca
     1981 tctctacata atcaggatga tagtctctgc ttgttttttc aagtgcttct aaagtatttt
     2041 gcgccccatt ttgggtactc acacctctgt gagagacagt cactggtttt gtctgaacag
     2101 ctgaagtcag ataatccata ttgtaaagac agacgccaat accgaaaaaa actgcaaaaa
     2161 gagtcaaacc agtgtttttc agcgcagaga aacgcaagtt gggggcttgt gggataaacc
     2221 acttagggat ctcaggcaag aagccttcgt catccatgaa atcaacaatg atataaaaaa
     2281 tccctacggt agacatcacg atattgaata ataaaatccc ttgtaaaagc gtcatagcaa
     2341 acacagcgct gatcaatgca tagtctggga aaaattgttc aaccatactt tgtgccaaaa
     2401 taacaacgat atatcctaaa gaagaaagga gtaaaatcgt accgccaatc acaataaatt
     2461 ggcccgtgat ggccaaaaga cgcgatttgg tcaatgacca gctttcccga atggctgctc
     2521 gaaaaggacg atctcgtaga atcatttccg gtaaagcaaa aatcagtcga atcccaatat
     2581 agccaaggaa aatataaacg agtaaaaagg aactgacgat gatccaacga ttagcaaaaa
     2641 taaaatccat aatgaaagcg gggattttta tttttgacag tagatcagaa ttaaaactta
     2701 atccgctgat cggtagaatc aataaaaaat aagcaaggaa aaacaaaaaa gtaatgggtc
     2761 tcactttctt cagctgaagt atcgtcaagt gtaagagttg cttcaaagag attggttctt
     2821 tctttttaat aaaaaaaaca ctcatcaaaa gaaaagtaaa ttcgaaataa actaacagta
     2881 aaatcgtcat caaaactacg attaacgaga gtagaacccc aggatgttga gaaaaaattg
     2941 aaggaatatt gtcataagac aaatagtcaa ttgcccctcg gtgtaaaata aaacgggtcg
     3001 tactgctcaa taagggtaaa caaataaata aaataaagcc atgcatcagc aagacatcac
     3061 ggaaataagc ttgtgttcct tttaaaaagt cccaagcatt acctaagcta cgtttcaaat
     3121 aattcatcct atcacctgct cttcacattg tagcgcaaat ctaatgaaaa aggaaagggt
     3181 gcagtgtgtt ttgcagaccc tttcccagat ttaacattgt caatcaatga ctaattttac
     3241 aaaataaata ttatccaaga agaccactta gatattgacc tccataaatc aaggccatac
     3301 tatataagaa aatcgacagt ggtttagcaa tcaaaatgat tagtgtatat cgtttcactg
     3361 acatgtttgt caatccagcc atcaaacata acgcatcgtc aggagcaact ggaagaaaaa
     3421 tagctagagc aaacagcttc tcaaaacggt tttgattatc cagccagcca atatatttgt
     3481 tatatgtctt atcgctaatc atggaaagta tgaatggctt tccataacga cgccctagca
     3541 gaaaaatgat aatcgaacca atgcagattc caatatagtt atagataaat ccagcataag
     3601 ggccaaatat caggacacca gctgcagtac tgatccctcc aggaatgatt ggaataacta
     3661 cttgcaggat ttggatgaag acaaaaataa tcggtcctaa aatgatggaa tcacctacta
     3721 atcctcttaa cgcattcaaa tctttgaaaa cacctaaatt aataaaataa atcgtcagcg
     3781 cgattgatag gcctaatccg ataatggata tgaaatttat cagttttcga gaggcagcaa
     3841 tattcattat tcatcaactc cttagaggtt ctttgttaac tatactttac tagaaaagca
     3901 actaactgcg ccattttttg atatgacaga aaacaaacaa ttttgtaagt tcatcataag
     3961 tataaaagat tgatctcaaa aaaacatcca actaaagact tatttaataa c
//
LOCUS       DACDMM010000199         4001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDMM010000199 DACDMM010000000
VERSION     DACDMM010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.202"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(111..1616)
                     /locus_tag="IWD16_002566"
     CDS             complement(111..1616)
                     /locus_tag="IWD16_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9089551.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1613..2299)
                     /locus_tag="IWD16_002567"
     CDS             complement(1613..2299)
                     /locus_tag="IWD16_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9089552.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2492..3913)
                     /gene="gndA"
                     /locus_tag="IWD16_002568"
     CDS             complement(2492..3913)
                     /gene="gndA"
                     /locus_tag="IWD16_002568"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP9089553.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1099 a          850 c          687 g         1365 t
ORIGIN      
        1 tttaattgtt cgctagtttt tttataatga catccctgaa tttctcactt gcttgtattt
       61 tgtaccttat tctttgttgg atatataaaa aaacaagtaa agcacatgct ttacttgtct
      121 tctgcctttg cgataggaat aaagattcta aaaattgtcc cttgccctac tacactttct
      181 gctaagatac gtcctttata gttttcaacc agttgtttag cgatagacaa ccccagacca
      241 tttccaccct tcgttctagc tcttgcttta tccactcgat aaaaacgatt gaagatctta
      301 ttaaggtctt ctttgggtat tccttcgcca aagtcctgaa tcgcgatctc aaattcattc
      361 agagtggaag aaatagagat atggacttct tttcgtgtag ttgaatactt tactgcgtta
      421 tccaatatga taatgattaa ttgttcaaag tgattacgat aaatttgcag ggtcacttct
      481 tttgtcaaat catcatctaa agtgatcaca tagtctggat ataatatctt gaaattatta
      541 taaacttgat aggtgacttc ttttccttta gttgtttcat tcgcataatg aacatctact
      601 tgttctgctc ttgataagtc tagcatctct tgaactaagc ttttcatacg gctgatttct
      661 tgcatgctgg cgcttaatga ttcttctaat atctcaggat catctttccc ccaacgattt
      721 agcagactaa gatgcccttc aataattgca acaggtgtcc ttaactcatg agatacgtct
      781 tccacgaatt gctcttgttg ttcaatatac aaacgcatac ggtcaagcat ctcattgaat
      841 atttcagcta aatctgccaa ttcatcattt gtgtcgattt caggcatgtg gatatctgat
      901 tgcggatctt tacgtatagt atccattgta tctctgagta ccttgagtgg ttttaagaaa
      961 taagaagata aaaagaaacc taaaatacta cttaagatca aagaaacaac ttctaaaaca
     1021 ataagagtca ataatagctt atttcgaata tcatagaaag aagataattc atagaagctt
     1081 tgcgcatagc cgatcttttc tcttgtacct ttagaatata taggctggat agataaaaag
     1141 ccagtttttc cttctagtgt aacgatcgtt ggaactttac gtgtagtctg aatcaggttt
     1201 cgactgtttt catgggtttt aaaaatcaag ttctcgtcca aatcataaac ataaagatcc
     1261 attgatggct gccctagctc agctataaag gaatcgatct tcattaagtt tccttcgacc
     1321 agtgaagtat gatctacgac ctcatctccg tcatttgtcg ctttcgtcag attacgatac
     1381 gtatttacta atgtcagttc ttcatccgag ttagccaaac gagaagctac ttctgaaatc
     1441 gtacgctcta cattgtttct ttcttttgcc acaatcagat tgatagatga tttataggta
     1501 atgacagcga aaattgtaaa cacaacgaat atgaagaaag aactcgcaaa ggcccacttg
     1561 attgtcaagg agggcccttt cagttctctt ttttccgatt ttttcggctt tatcacgagc
     1621 gcatcacata acctgttccg cgtacagttt ggatatagct ttcctccccg ggaacgtcaa
     1681 ttttgttgcg aagataacga atatatacgt ccactacgtt tgtttcaact tctgtttcgt
     1741 atccccacac tttatttagt aaaacgtcac gtgctaatac cacgttcacg ttttccatta
     1801 aagtaagcag caactcgtat tctctttttg ttagttcaat tacttcattt ccgcgacgta
     1861 cgacacggtt ttctttttcg atagtcaaat cgcgataagt aatagtagtt tgtttagcca
     1921 catttttatc gccttcaata tcgattcgac gcaacaatgc acgaagacgt gctaataatt
     1981 cttcgatagc aaatggtttg acgatgtagt catctgctcc gtggtcaagt ccagatacac
     2041 gatcgatcac agaatctctt gcagtcatca taatgattgg tgtatttttg acttggcgta
     2101 cacgtctgca gacttctaaa ccgttcaatt ctgggagcat cagatccaac aatatggcat
     2161 cccactcatt atttaatgcc gcatctaaac cagttcggcc gttgtagtgg acttctgcgt
     2221 tatacccttc atgcttcaac tctagctcga caaatcgtgc caagttcttt tcatcttcaa
     2281 tgattagaat attgctcatt tattatttta tcctttctat tctactggct ttttaaaaac
     2341 tctatcatta taccgataat atgcttaaaa atctatagtc attttatcat ggatagcaat
     2401 tttaaaaaag agctgaagtc tactcataac aatcgacttc agctctttcg tcctcttttt
     2461 attttctcat ttcctaacag ccgaaagact attattcttc atggtaccat gagtaatgga
     2521 agattccttc tttgtcgaca cgctcatagg tatgtgcgcc aaagtaatcg cgttgggctt
     2581 ggattatatt tgcaggcaga cgttctgctc tgtatgaatc gaagtaagcg atggctgatg
     2641 agaaagtcgg tacaggtacg ccagcttgga cagccaaggc aacgacgtct ctgactgatt
     2701 gctgatattt tttcgtgatt tcaacgaagt aatcatctag caataaattt tcgatctcag
     2761 gatttttgtc ataggcatcc gtaatttttt gcaagaatcg agctcggatg atacatccag
     2821 cgcgccagat ttttgcgatt tcaccaaatg gcaagtccca atcaaattct tttgaagcga
     2881 cacgtaattg agcaaatcct tgggcataac tcatgatttt actgaaatac aacgcttctc
     2941 ggattttttc gaccaattct tttttgtctc ccgtgaactc aaaatcgttc gtacgagaca
     3001 aaatcttgct tgcttgtacg cgttcttctt tataagcaga aatatagcgg gcaaatacag
     3061 attcagtgat caatggaagt ggcacaccta agtcaagcgc gctttggctt gtccatttac
     3121 ctgttccttt attcccagct gcatcaagga tcacatctac tactggttta cctgttcctt
     3181 catcgtcttt acgtgtcaga atgtcagcag tgatttctat caaatagctg tctaattcgc
     3241 cttgattcca ttctttaaag atatcagcca tttcttctac tgaaaggttt aaaatattct
     3301 tcattaagtc ataagattca gcaatcagtt gcatatctcc atactcaata ccgttgtgta
     3361 ccattttcac ataatgtccg gctccatttg ggccaatata tgtgacacat ggtgctccgt
     3421 cttctgcttt tgcagaaatt tgctctaaga ttggtgcaac tagcgcatag gcttcttttt
     3481 gtccaccagg catgattgaa ggtcctttta aagctccttc ttcaccaccg gaaacgcctg
     3541 taccgataaa gttgatgcca gagttggcta actcttcatt acgacgcatc gtatctttaa
     3601 agaatgtatt tcctccgtca atcaaaatat cgcctttatc aaggtgagga agtaactctt
     3661 ggattgttgc atctgttgcc ggtcctgctt tgaccattag caagatccga cgtggtttct
     3721 cgatagattc tacaaattct tcgattgtat atgttgcttt gaaattttta tctgggtgtt
     3781 cttctactac agcggttgtt ttagcccctg tacgattgaa taaagccact gaatagccgc
     3841 ggctttctat gttcaaggct agatttttac ccataacggc cataccgaca acgccgattt
     3901 gttgttttga catatgaact cctcctacat acgggatttc tccctaatct cttacattat
     3961 agcttaacac ggctacattg caaactaaag aacaacatat t
//
LOCUS       DACDMM010000200         3900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDMM010000200 DACDMM010000000
VERSION     DACDMM010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.203"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            175..519
                     /locus_tag="IWD16_002569"
     CDS             175..519
                     /locus_tag="IWD16_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP9089554.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            520..939
                     /locus_tag="IWD16_002570"
     CDS             520..939
                     /locus_tag="IWD16_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9089555.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1031..1381)
                     /locus_tag="IWD16_002571"
     CDS             complement(1031..1381)
                     /locus_tag="IWD16_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089556.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1347..2567)
                     /locus_tag="IWD16_002572"
     CDS             complement(1347..2567)
                     /locus_tag="IWD16_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP9089557.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="IWD16_002573"
     CDS             2933..3499
                     /locus_tag="IWD16_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9089558.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="IWD16_002574"
     tRNA            3644..3731
                     /locus_tag="IWD16_002574"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
BASE COUNT         1429 a          669 c          760 g         1042 t
ORIGIN      
        1 aaaataaaaa aagcgttagc aactatgctt aaaaagcata tcgactgaat aaactaagca
       61 ttggacaggc tgaaagacta catttaaaat gaaatgcgaa gaaacaatag aaaataaagt
      121 tgccttagaa ccatagttga atcacctaac agtcgtgaag ggagaataag agaaatggca
      181 gaaaaacaaa ccgcaggacg agatcaatta ggagattttg caccaaaatt tgcagaattg
      241 aatgatgatg ttttatttgg acaagtatgg tccagagaag aattatctgc tcatcagcgt
      301 agtatcatca ccatatctgc attaatttca ggaggaaatt ttgaacaatt gccagctcat
      361 ctgaagattg gcaagcaaaa tggcattact agtgaagaaa tagcagaaat cattacccat
      421 ttagcttttt atgtaggatg gccaaaagct tggtcagctt ttagtttagc aaaagaaatc
      481 tatttagaag aaaatagcga agataagaaa gaggcgtaaa tgacgacaaa acacgaagaa
      541 atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg aagcttacgc tcaagtattt
      601 accgggcaaa gctatctgaa tatgttagtg aatgatcccg aagtttctgt tggtgtaggt
      661 aatgtaattt ttgaaccagg ttgtcgcaat cactggcatg tccataaaga cggctatcaa
      721 atcttacttg tgaccggagg agaaggctgg tatcaggaag aaggaaagcc agcaagacga
      781 ttaatggctg gagatgtcgt cgtaacgcac aaagggatta aacattggca tggtgcgaca
      841 aaagacagtt ggtttgaaca tttggcgatc actgctggta attcagagtt tttagaacct
      901 gtaacagatg aagtgtatga aggattggaa aatcaataat gtcaagcttg aacagctcct
      961 gaaagtagat tttaatcgaa ctttcaggag ctgctttttt gattcagtct tttataaata
     1021 gaaaataggg ttaataatag gaatgagaag cacgtcgaaa gatcttaata ttgataatcg
     1081 gctgggcata catgatttcc acgtcatcaa tcaaactacc taacagatga taatcttccc
     1141 gatcatgtcg gggattacct aatagttctt gatagtagat ttccatacta gggtcccgag
     1201 gattgttcag caaatcagca agtacatcta aatcttctgg ctgagtttct gccgtaccct
     1261 gtatctctat gaaaagccct tcctcagcaa attctactcc aatacgtatt tcagttattt
     1321 catgggaata taaataagta agaagctcat cgatgattct ttttgataat tctttttcca
     1381 agtgtctttt tttcctttct gacagcggta aagttatcta gtatcggtac aaggatacct
     1441 gcaacgaaac cagttgaaaa accactgtta tatagattta gtcctccgtg caaagtgctg
     1501 acgttatgga caagagcaat atgtagtaca ccagctatta caccgtaaaa actgccgtaa
     1561 aaaccgctta tgggcgcgag acctgtccca aacacagctg caacaagcat gccagtactg
     1621 gtgatctcat gaaaagttcc aaacaaagaa gcaataaata ttccgactag tatcggaaaa
     1681 ctgtttttta aatgacaacc aaaagcactg aaacccaccg cagataaaat agcaccgatg
     1741 acaggtccat ttaactgacc gccaaccagc aaaacataag aagacagcat cagaccaact
     1801 aagcctatgt tgatcaaagt agcaccaata ccagaattag caatgaaatc agttgtcaat
     1861 tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc gaaaggaatt ataatagaaa
     1921 ccaattccga ctgtcagcga aaaaaagcca aaaataatcc aaatgagagg aaagtgatat
     1981 tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga ataacctcaa aacagcagta
     2041 aaagtcatcg caatcaaccc agaggtgaat ccaatattat aaagtgtgaa tccacgatgg
     2101 aaaactaaag tttgagcagc taagggcgga agaagtagac caattgctat gccagctaaa
     2161 ttccccaata aaatacctat cagtaaagga aaacgcatac caaaagtgat ataactaacg
     2221 accggtgata atgcgctacc aaacagtccg atcatgatgt actgagaaaa aggtttattg
     2281 acaaatacag cgtatagata cacacctaaa atgattgaga tagaattata aaggtttttg
     2341 ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa ataagggacc gtttaaaatc
     2401 accttgtgcc gataagctaa aagcacagaa agtaaggtca aaatccctac atttatgaat
     2461 gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa gattgcttgg cgaggtaaga
     2521 atagttagca atccactcca attatcagta ggagtattag aaagcaagcc aataaaaaga
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
//
LOCUS       DACDMM010000201         3876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDMM010000201 DACDMM010000000
VERSION     DACDMM010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.204"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            158..2257
                     /locus_tag="IWD16_002575"
     CDS             158..2257
                     /locus_tag="IWD16_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9089559.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="IWD16_002576"
     CDS             2453..3316
                     /locus_tag="IWD16_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9089560.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3876
                     /locus_tag="IWD16_002577"
     CDS             3421..>3876
                     /locus_tag="IWD16_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9089561.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1544 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaaa
//
LOCUS       DACDMM010000202         3797 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.205, whole genome shotgun sequence.
ACCESSION   DACDMM010000202 DACDMM010000000
VERSION     DACDMM010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.205"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            46..2391
                     /locus_tag="IWD16_002578"
     CDS             46..2391
                     /locus_tag="IWD16_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9089562.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            complement(2728..3729)
                     /gene="ccpA"
                     /locus_tag="IWD16_002579"
     CDS             complement(2728..3729)
                     /gene="ccpA"
                     /locus_tag="IWD16_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP9089563.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
BASE COUNT         1219 a          759 c          728 g         1091 t
ORIGIN      
        1 tttgctataa ttagcgaaat aaaaccatta gttgaggtga cccttttgcc acgcaaaaat
       61 actcgaaaaa aacagaatag aagaaaaaaa gaaaaatggt ttgttcctaa gattattttt
      121 agagtttttc aatcattgac tgtttttatt actgttcttc ttttgatgtt cgcagctcta
      181 ggtattggaa taggcgctgg ctattttgcc tatctcgtcg aagacacaca attgccgact
      241 aaaaaagcac tgcagacaga actaggaaac attacagaaa cgtcaaagat tgtttatgca
      301 gataatacag agatatcaaa aatccagaca gatctgatgc gcacgactat ttcatctgat
      361 aagatttcac ctttattgaa aactgcaatt atcagcacag aggatgaata cttcgataaa
      421 caccaaggtt acgttcctaa agctgtttta cgagcattag tatctgaagc tactggtatt
      481 ggctcttctg gtggatcaac tttgacacag caattagtca aacagcagat ccttacagat
      541 gaaacaacat tcaaacgtaa agcaaacgag atcattcttt ctgcacaagt agaaaaatac
      601 ttttcaaaag atgagatcat tgctacgtat ttgaatgtct caccatttgg tcgtaataat
      661 aagggacaaa acattgctgg agtccaggaa gctgcaaaag gaatctttgg tgtagatgca
      721 aaagatgtga cgctgccgca agctgcttat atcgctggat taccccaaag cccgatcacg
      781 tatagtccat acacgaacac aggcgcgcta aaagaagatc tttctgccgg ccttgccaga
      841 aaagattttg ttttattcag tatgtatcgt gaaggccaaa ttacgaaaga acaatacgaa
      901 gaagcaaaag cctatgacct tacaaaagac tttctaccac aacaaatcgc agaacagact
      961 gacagagaat tcttgtatta tactgtgatg aatgaagcaa caagaatcat tgcacaacaa
     1021 ttggctgaaa aagacaacgc tgatatgtct gattcatcgg tcagtgacgc ttattatcaa
     1081 aaggcacagc aaacgatcca aaataaagga tatacaatcc attcgacaat cgataaagat
     1141 gtctatgctg cattgcaaaa tggcgttgaa aattatggat acatgttaga tgatggtgct
     1201 ggcagtcaag tagaaaccgg aaatgtattg atggataaca gaactggccg catctacgga
     1261 ttcgttggcg gacggaatta cagtcagaat caaaataacc atgccttcga tacagaacgt
     1321 caagctggct catcaatcaa accagttctt gtttatggac ctgcaatcga catggggctt
     1381 atcggatcag aatcccgtgt atcagattat gcaacaactt ggcaagaagg agaaaatgct
     1441 ggcgagaaaa tcgtcaatgc tactaacgaa ggatcgaaca ccttccaaac cgtccgtgag
     1501 tcactagaat ggtccaacaa tatcccggct tatcacttat atcaagatgt gttgaattcc
     1561 ggtggatcga aacaatatgc ttacgagcac tacttggcaa agatgaacta cccggcaaat
     1621 gacaattggg gcgttgaatc tgctccttta ggtacagtag atgtcacgac gcttcaacag
     1681 accaacggat ttcaggcttt agcaaacggc ggcgtctatg aagaaggcta tatcatcgat
     1741 tccatcacag ataacgcagg aaatgtaatt tataaacacg aaagcaactc tgtccggatc
     1801 tattcggaag caacggcatc gatcatgaat gacatgatgc gtagtgtgat caatgcaaaa
     1861 atcactacac cattcaaaga tgccatctca agcttaaacg gaaatctagg aaaagcagat
     1921 tgggtcggaa aaactggttc taccaatgaa taccgagatt catggctaat tgtctctact
     1981 ccgtctatta ccatcagtag ctgggcaggg cacgatgaca atactggaat ggacagcaaa
     2041 gcaaggatac gttcggctaa ttatcttgcc aatctgatca atcaagctta ccaagcaaaa
     2101 cctgacattt ttggcactga tgagaaattc gaattatcat ctgatgtgat gaagaaaaaa
     2161 gtatctggat ttactggtca aacacctgga aaagttactg tcaataaaaa atccatccaa
     2221 acaccaggaa aaacagttga atcgctatgg gcgaaaaatg gaccgaagaa aagcgccttt
     2281 aaatttggcg ttggcggtac cgatgaaaac tatacagatt actggaacac cgcttcagct
     2341 tatgcaagag cgaatcaagc aactaaagaa tcagatgaga aagatgattg atttaataga
     2401 atacacagta ataagggatt gtgacaatag tattgtcaca gtcccttatt tttttgatgg
     2461 tggtgaacaa aatgcagcat ctatacacgg attgttttat atacgataaa tgtaatttca
     2521 accactttct ctctttttta agacacaaaa aaaggattat aacaaaattt tgtcataacc
     2581 ccttttccca aataaacggt gttctcctgc caactccgaa tcacgagtca caatattcat
     2641 caaacatgag atgctgtttt ttgaaattgc tccttgtaac gcagagttaa acggcaggca
     2701 cacaacctct gaatggttgt ccttttatta ttttgtagat cctttttctt cgattccata
     2761 aggcaagata atcgtttttt cttcgacttc ttctttattc atcattttcg tcaataggcg
     2821 catagatacc gcccctaaat cgtataatgg ttgtgtgata cttgttaaac gaggacgagc
     2881 tacttctgtc agtagtgaat tgttacttgt gatgatttcg aattcttccg gtactttcac
     2941 gccacggtct aataaaccat ccaaaatacc gatggctaat tcatcatctg aaacatagac
     3001 agctgttgca ccgctattat ggatccgatc gactagagat agaccatctt tgaagttgta
     3061 ttgtgcttca aagatcaatc cttcgctata tggtaggtta ttttcgctta atgcttcttt
     3121 atagcctttc agacgatttt gtccattgat gggatcgatc aacgcaccac tgacaaaagc
     3181 gattttttta ttgccgcttt ttgccaattt gtttactgca tcttttgttg cggatacata
     3241 atcaatgttt acgctaccta cttgttcatc tggatcaata gagccagcta atacaacagg
     3301 agtttttgaa cgtgaaaatt ctccacggat ttcgtctgtg atatggtgac ccatgaagat
     3361 cacgccgtcc acttgcttag ccagtaagtt attcaataca ttgacttctt tttggtcgtt
     3421 gccatctgaa tttgctaaga taatattgta tttgtacatt gttgccacat catcgatacc
     3481 acgtgctaaa gaagcaaaga acatattgct gacatcaggg atgattacac caactgttgt
     3541 tgttttctta cttgccaagc cacgcgcaac tgcgtttgga cgataatcca agcgatcgat
     3601 aacttccaag actttttttc ttgttgctgg tttaacgttt ggattgccat ttacgacacg
     3661 tgaaacagta gccatcgaaa catttgcctc tctcgctaca tcatagatag tgattgtttg
     3721 cttttccatt tgtgttctcc tattcatatt ttatatgttt tctgaaaata cgtcttacat
     3781 ttctaaaata gaatatc
//
LOCUS       DACDMM010000203         3713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDMM010000203 DACDMM010000000
VERSION     DACDMM010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.206"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IWD16_002580"
     CDS             complement(162..2159)
                     /gene="tkt"
                     /locus_tag="IWD16_002580"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP9089564.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2261..2512)
                     /locus_tag="IWD16_002581"
     CDS             complement(2261..2512)
                     /locus_tag="IWD16_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP9089565.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2659..3282
                     /gene="lexA"
                     /locus_tag="IWD16_002582"
     CDS             2659..3282
                     /gene="lexA"
                     /locus_tag="IWD16_002582"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP9089566.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3462..3680
                     /locus_tag="IWD16_002583"
     CDS             3462..3680
                     /locus_tag="IWD16_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089567.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          797 c          688 g         1203 t
ORIGIN      
        1 ctcaagaaac acaaactaat ccgaagaatt ctttgttttg tcatttaaat gtaacatagc
       61 cgaaaacgaa aagtaatatt gatttattct tctaacacat aaagaacagc tgtctccaac
      121 ttgcagacag ctgttcttat cagtcaaaca attgcttgga tctatgatag ttgattgtac
      181 gtattcacga cattttctac tgtaaatcca aattcttcta aaattttagt gccaggggca
      241 gatgcaccga aatgatcgat tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat
      301 ccaaatgatg cagcagcttc gatagccaca cgtttcttca ctgatttagg aagtacactt
      361 tctttatatt cagaagattg tttttcaaag aggtcaaagc ttggcataga aacaacagaa
      421 acatctttgc ctttttcagc caattctttt tgagctttca cagccaaatc aacttcagaa
      481 cctgttgcaa tcaaaatacc ttctggtgtt tctccttgtg atggagaaag aacatacgcg
      541 ccttttttca ccatttcatc tgctacttct tttgtagaag gcaatactgg aaggttttga
      601 cgactcaata caaggatagt aggcgcatct gttgtctcca tcgctacttt ccaagcagca
      661 cgtgtttcat ttccgtctgc cggacgaatc acttgaacac caggcatgca gcgaacagat
      721 gctagttgtt caatcggttc gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt
      781 gttaagacgt aaacaacagg agtatgttgg attgctgcta aacgaactgc tggtcttaga
      841 taatcgacaa agacaaagaa tgttccaccg tagatacgag ttccgccatg aagttggata
      901 ccattcatag ccgaagccat tgcaaattcg cggacaccga accagatgtt gcgtccttca
      961 taatgttcag gagtgaaatc tttatctgca gccaccattg tattattgga acctgacaag
     1021 tccgctgaac caccccagaa gcttggtata gctttcgata attcttggat gacttcttta
     1081 cttgaaacac ggcttgcttg actgcttcca acttcataag ttggcaattc tgcatcccag
     1141 ttttctggta gtttgccatc aaaagcatct tcaaattgtt gtgctaattc aggataagct
     1201 tttttataat tggcaaacat ttcattccat gcatcttcag ctttttgtcc ttcttctatc
     1261 atcgtttgat ggaagcgggc agctacttct tctggaacag tgaaatctgg atactcccaa
     1321 ccataaactt ctttagccat cttgatgcca tcttcaccaa gaggagcacc atgaacagca
     1381 gatgttcctt ctttaggagc accatagccg atgactgttt ttacttcgat caatgttggt
     1441 ttgtctgttt cagcttttgc ttcttcaatc gctttagaga ttgcttctag gtcatttccg
     1501 tctttcacta agatatgttg ccagccatat gcttcatagc gagcaccaac gttttctgtg
     1561 aaagcttttg aagttggacc gtctagtgaa atatcgtttg aatcgtataa aacgatcaat
     1621 ttccctagtt tcatatgacc tgccatcgaa cttgcttctt gagatacccc ttccatcaag
     1681 tctccgtcac cgcatattgc atacgtatag tgatccatca cattaaagtt ttctttgtta
     1741 tacgttgctg caaggtgcgc ttctgccatt gccataccga cagccattgc aatcccttgt
     1801 cctaatggcc ctgtagtggc ttctacacca tctgtatgat gtacttcagg atgtcctggt
     1861 gttttagaat cccattgacg gaattgtttc aaatcatcga ttgtcacttg atagccagct
     1921 aaatgaagca agctatacaa cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg
     1981 cggtcagccc agttttttga tgtcgttgga tttactttca ggtgttttgt ccataacgca
     2041 tatgccatag gtgctgcgcc catcggcaat ccaggatgtc ctgagtttgc tttttggact
     2101 gcttcaatac ttaatgtacg aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa
     2161 ataacccctc ctattaattt aggttttttt tacctttatt tcttacactt tcattctata
     2221 agatgtcaca cgaaaaagca atctttttca gtatttttta ctaaaagttg tcatttcggt
     2281 tgtgtaagcc ttttttcttt tggatttctt ttaatttttc cggggtcacg tcattgccct
     2341 caggatcgac gactttcatt ccttcgatat gatgacgcat accggaacga aaagcttcca
     2401 aatactcttt gcgcaagttg atttgttctt gttcttcggc ttttgagaga ccattttcct
     2461 tctttttacg tgcaagttca ttgatccgct ctattttttc aggtgacagc atgctcagcc
     2521 ctccttttac tctccttcat attatataaa aactgaaaaa aaaacaacta gataaactaa
     2581 ataaaatacg aacattcgtt tgatttagcg ctttttatat ggtactattt atttatcaag
     2641 tgaaagaagg tgtaccttgt ggcccgacaa actgaatcaa gacaaatcga agtcttacga
     2701 tatatccatg aacaagtaac ccaaaaagga tacccgccga ctgtccgtga aatcggggaa
     2761 gctgttcagc tttcttctac ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa
     2821 gggtttatcc aaagagatcc gacgaaacct cgtgcaatcg agttgacaaa acaaggttta
     2881 gaaaagattg ggattcgtcc aacaactatt ccagtacttg gcgtcgtaac agctggagaa
     2941 cccattttag cagtagagga tgcttcagac ttctttccaa tccctcctaa tctttcttcc
     3001 gaagaaggca gtctctttat gctgacaatt cgtggagata gtatgatcaa tgctgggata
     3061 ttggatggtg acaacgtgat cgttcggaag caagaaactg ctcaaaatgg cgatattgtc
     3121 attgcaatga ctgccgagga cgaagcgact tgcaaacgat tctataaaga aaaaaacttt
     3181 ttccgtctgc agcctgaaaa cgacttgctc gaaccaatca tcttagatca agtatctata
     3241 ttaggacgag tagtaggact ttatcgtagt catatctatt aattgctact aattaaaaga
     3301 aaacttttta aaaagagttt gagacgttgg tctcaaactc ttttttcgcc cgggttcttc
     3361 ttctttcaaa tttttatcac ttccctcttt acaaaccgaa caaatattcg tatactaaat
     3421 atacgaacaa ttgttcgcat aaattaaaga ggtgagaatt catgaaatca atgagacgtc
     3481 ttggcttagt attaactgtt ttgtttttag gtattattat tggtaatttc ggcatacgtc
     3541 cagctttact tgtgtttgtt ccgttattta ctttgtggtt catgttgtgg gatgaaaaga
     3601 agtaccgtca atctgagcgg cgcagaattc atgaagatca tttctatcat gagccctact
     3661 acagatccag gcaatcataa taaggattga ctgctaatta ttcaaaagac aag
//
LOCUS       DACDMM010000204         3691 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDMM010000204 DACDMM010000000
VERSION     DACDMM010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.207"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            75..470
                     /locus_tag="IWD16_002584"
                     /pseudo
     CDS             75..470
                     /locus_tag="IWD16_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            790..1104
                     /locus_tag="IWD16_002585"
     CDS             790..1104
                     /locus_tag="IWD16_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9089568.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="IWD16_002586"
     CDS             1208..2386
                     /locus_tag="IWD16_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9089569.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="IWD16_002587"
     CDS             2789..3082
                     /locus_tag="IWD16_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP9089570.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1259 a          546 c          716 g         1170 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactctttt attcttccct catagcttcc a
//
LOCUS       DACDMM010000205         3588 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDMM010000205 DACDMM010000000
VERSION     DACDMM010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.208"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            41..>3588
                     /gene="esp"
                     /locus_tag="IWD16_002588"
     CDS             41..>3588
                     /gene="esp"
                     /locus_tag="IWD16_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP9089571.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDIIENPGDNIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPEDKLPVL
                     KAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVTFTAGEG
                     TSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATK
                     LDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKK
                     GTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT         1347 a          577 c          763 g          901 t
ORIGIN      
        1 cttactagga aagtaattat taagggagtt gatttgaata atggttagca agaataataa
       61 gagagtattt ctagaaaaaa caaagaaacg agtattgaag tacagtatca aaaaattaag
      121 tgttggggtt gcatctgttt tgattggtgt tggtcttgtc ttgggaacaa ctgaactagt
      181 taaagcacaa gatgaaattt ctccaagcag cccacttgaa acagcgataa gctccgtaca
      241 agtaggtgac aaagtagctt cggggaatac ttttcaggag aatcctggtt atacgaaaaa
      301 ttataacttt tcggatttac aatttagtcc tcaagaacta actggagaca cactaaaagg
      361 caatacaatt ggatttgaag tttacggaaa acataatatt gcagcatcaa ctaaaaactg
      421 ggaaatccgt cttcaattag atgaacgatt agctaaatat gtagaaaaaa ttcaggtcga
      481 tccaaagaaa ggaattggat cttctagacg gacctttgta agaattaatg attcgctggg
      541 tagacctaca aatatttgga aggtcaatta tattcgtgca agtgatggat tatttgctgg
      601 ggcagaaaca accgatacgc aaaccgctcc taatggggta attacgtttg aaaagagcct
      661 agatgaaatt tttaaagaga taggcataga caatctcaag accgatcgtt taatgtatcg
      721 aatctacttg gtaagtcatc aagacgatga taaaattgta ccgggtattg atagtacggg
      781 ctacttttta acagattcag atgatttcta taatagctta gatgtatctg aaaataatcc
      841 tgaccagttt aaacatggat cagtaagtgc aaaatatgag aaacctaata ctcaaacaaa
      901 agatggttca ggatccacag gtgctaatgg agctataatc ttagatcata agttaacgaa
      961 aaattataat ttctcatatt catcttccgc aaagggcacg ccttggtatg ctaattacaa
     1021 aattgatgaa cgattagttc catatgtagc gggtatacag atgcacatgg ttcaggctga
     1081 caaagtaaca tatgatgttt cgtttgaatc cggaacaaaa gtagctgatt tagcaatcga
     1141 gcgacgggaa ggtcatgaaa attatggcat aggttcaata actaataatg atttaactaa
     1201 acttatcgat tttgctaatg caagtccacg tccagtcgtt attcgatatg ttttacaatt
     1261 gactaagcca ttagatgaaa tcttagaaga tatgaaagca acagcacaag ttgaagaaaa
     1321 taaaccattt ggtgaagatt tcatctttga ttcttggttg tcggatacga ataaaaaatt
     1381 aatccagaac acttatggaa caggttatta ttatttgcaa gatattgatg gtgatggaaa
     1441 ccctgacgat aaagaagaga gcggagacac gaatccatat atcgggaaac ctgaattaga
     1501 agaagtatat gatgttgaca caacagttaa ggggaaagta ttcatccacg agttagcggg
     1561 aacaggtcac aaagcccaac ttgttgataa agaaggtact gtattagcag aaaaaactat
     1621 cgctccaaat gaaaaagatg gggctccaat ttcagatact gtagaatttg aatttacggg
     1681 tgtagattca agtaaattaa tcgcgaaaga tgaattaaaa atccaaatcg tttctccagg
     1741 ttttgataaa ccagaagaag gttcaaccgt tattaaggaa tcaccaaaag cggttgataa
     1801 acaaaccgtg gtagttggat ttaaaccaga tgctaaagaa tcaattcgga ataataaaaa
     1861 cttacctgaa gatgcagagt attcatggaa aacagagcct gatacttcta acgttactga
     1921 tagtacgaaa ggtattgtaa ctgttaagat cggaaatcga actttcgacg tggatgtaga
     1981 gtttgctgta aaagcttctc aagctatgga aaatgatgca acatacgtac ctataacaac
     2041 aactccagaa acgacagttc aaagtggaaa acctacattt gataaaccag atgttccttt
     2101 agctaaagat gccttttcaa tttcagatgt ctataataag tacttcggta atgcaagtgt
     2161 tgatgcaaac actggtattg ttacattcac tccagctaaa ggtgtaggag aatcggagcc
     2221 gattactgga acaattccta ttaaaattgt ttaccaagat ggttctgtag gcacgaccga
     2281 tttagcagta actgtaagta aagatattta tgaaaatcca ggagaaaaca ttcctgcagg
     2341 ctaccacaaa gtaaccttca ccgcagggga aggaacaagt attgaaagtg gaacaacagt
     2401 ctttgcagtg aaagacggcg taagcttgcc agaagataaa cttccagtgt tgaaagcaaa
     2461 agatggttat acagatgcga aatggccaga agaagcaact caaccaatta cagcagatga
     2521 tacagaattt gtatcaagtg caacaaaact ggatgatatc attgaaaatc caggagaaaa
     2581 cattcctgca ggctaccaca aagtaacctt caccgcagga gaaggaacaa gtattgaaag
     2641 tggaacaaca gtctttgcag tgaaagacgg cgtaagcttg ccagaagata aacttccagt
     2701 gttgaaagca aaagatggtt atacagatgc gaaatggcca gaagaagcaa ctcaaccaat
     2761 tacagcagat gatacagaat ttgtatcaag tgcaacaaaa ttggatgata tcattgaaaa
     2821 cccaggggat aacattccgg ctggctatca taaagtaacc tttactgcag gagaaggaac
     2881 aagtattgaa agtggaacaa cagtctttgc agtgaaagac ggcgtaagct taccagaaga
     2941 taaacttcca gtgttgaaag caaaagatgg ttatacagat gcgaaatggc cagaagaagc
     3001 aactcaacca attacagcag atgatacaga atttgtatca agtgcaacaa aactggatga
     3061 tatcattgaa aatccaggag aaaacattcc tgcaggctac cacaaagtaa ccttcaccgc
     3121 aggagaagga acaagtattg aaagtggaac aacagtcttt gcagtgaaag acggcgtaag
     3181 cttaccagaa gataaacttc cagtgttgaa agcaaaagat ggttatacag atgcgaaatg
     3241 gccagaggaa gcaactcaac caattacagc agatgataca gaatttgtat caagtgcaac
     3301 aaaactggat gataaatctg acgctgacaa atataatcct gaaggtcaaa aagtgactac
     3361 agaattgaat aaggaaccag acgcatttga gggaattaag aacaaagaag atttaccaaa
     3421 agatactaag tatacttgga aagaaaaagt agatgttagc gcagctggaa ataaaaaagg
     3481 gactgttgta gtgacatatc cagatggatc atctgatgaa gttgaagtag atgttacagt
     3541 gacagacaat cgttctgacg ccgataaata tgagccaaca gtagaagg
//
LOCUS       DACDMM010000206         3536 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDMM010000206 DACDMM010000000
VERSION     DACDMM010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.209"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            287..1327
                     /locus_tag="IWD16_002589"
     CDS             287..1327
                     /locus_tag="IWD16_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9089572.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2151..2483
                     /locus_tag="IWD16_002590"
     CDS             2151..2483
                     /locus_tag="IWD16_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9089573.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3102..3443
                     /locus_tag="IWD16_002591"
     CDS             3102..3443
                     /locus_tag="IWD16_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9089574.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1309 a          470 c          658 g         1099 t
ORIGIN      
        1 taatttattt tttgttttga cactggtagt acttaattat ctttaatttt aaaactaaat
       61 tttattcata cgtttttctg gtagtattta attataaata atatttattt aaagtagttc
      121 ttaatgactt taaaggtagt acataattca ttttatttct gattttaggt agtatttaat
      181 taaaaaacag ttttaatgcg tttgcatttt ttatttatct ttgctatttt cgaaaagagt
      241 aggtaatttt acataaataa aacaacgtga aagtagagga tctacaatga ctgattttaa
      301 attttttaag gcggatagag tttacaacga attattttat caatttccaa aagtctttat
      361 tgtttctgac gaatacaaaa aaatgaaaga ttcaactaag attgcctata tgcttttaaa
      421 agcaagatta gagatcgcaa tcagcaaacg gcaaatcgat gaagaaggta atgtttattt
      481 tacttatacg acaaatgaac tatgtagagt attaaactgc caaaaacaaa aagcgatagc
      541 aatcaaaaaa gagttggaat cctttggttt attattacaa aagcagatgg gatttaacaa
      601 acagttaggg aaaaataatc ctaatagact atatctagca gaattaaaag tctcagaaaa
      661 tgatatctac ttactcgaaa aatttgatag agagaatagg gaaaacgttg ataaatcaga
      721 gggtatgaaa atcataccca ccctcgacga aaaatcagac gctgaatccc ttggggctca
      781 agagggtatg aaaatcatac cgtgccaaaa cgttgataaa tcagagggta tgaaaatcat
      841 accagaactt aataataata tattagacac taatagacac aatatagaca ctgaaaaaga
      901 ccgcctacaa gatcaattgt tgttagacaa ttttgagaca attatgacaa acgacagcat
      961 tgctacgttt gtccctgaac gagtattaaa tttgataaaa acattttctt caagttacag
     1021 tgaagctcaa aaaaccgtcc agactattca taatgcaaag aaaaaagctg aaatagaaag
     1081 tggtatttcg atagtttttg aagaactcga tagttattat gtcaatgcag aacaagaatt
     1141 atacacgaca ctgttaaaag cctatcaaaa attaaaaacc gaaaaagtcg aaaatatcca
     1201 gaacctgatt tttgtctatg taaaaaattg gtttatcgaa aaaccaatag ctgctaaagt
     1261 atcaagtgaa aaacgtttga attatgaaag ctccccaagc actattacga aagactggtt
     1321 agagtgaaac tttactgaca aagtgattgt tgcttcatgg agtaacatta aaattaaaaa
     1381 ttttttatta ttctaattat attacgaata ttacaaaata atagctattt taatagaatt
     1441 tataagttaa aatattatga taaaaatcac aaacgtagcc ttttacgtca taagtgttaa
     1501 aagaaataat taatttcaaa tatagaaaag ggaacgtatg tttgcatact cctttttttt
     1561 gagttataat aatacgcgaa gagagataat ttcattattt aaacaagcta aaaatcttga
     1621 tattacttct tttcaggcaa tacgaatatt attcgcttac attttttgta taaatttaat
     1681 tgtctgctat gcttgtgatg aattgatttt cgttctgcac ttttggatga taaatttgga
     1741 caattgaaaa tacaaaaaaa agaaaggcgg tatgaactcg gaaggctggt aaccttcgtc
     1801 agcgttcata cacggcatta agatttggcc cttaatgcgt taacctttca gtgttacggt
     1861 attacccgca atggatttag tataacagat attcaaaagt gataccactt tttttggctg
     1921 tgttaaggtt aaaaatacgt gatattacga tgaatctatg cctaagcaca atgttataaa
     1981 aatgtccgtg tggctctttg ccgtatgacg aacgctgaaa ttagagggaa cgcatgtttg
     2041 gatgcgttcc ctcttttttg tttgtcatga acgaaaatca attctctatt tttgaaaaat
     2101 aacaagaaaa acttgttaaa aatatttaaa gaaattgggt ggtttatttt atgtcatata
     2161 caggaatttt gagtctcaaa gatatttgtc actacggtaa acggtgtacc gctacagaga
     2221 aaatcacgaa gaaactatca actggccaaa gtaaaaccgt tgtgcagtgt aagaaatata
     2281 taatccagaa agacaaagtc tcagaggaaa tgatttatta cattgggaaa caaaagcaaa
     2341 taattttaaa agatcctatc cccttaaaag agctatatcc aacaatcaaa catgtttatg
     2401 accaaaacgg tgtactgatt ggtcgaagaa aaaatggagt cttgcgttgt accgcaaaag
     2461 ggatggggcg tttgatcggc tgaattgatc attctcataa cttgtatttt atcgtttttc
     2521 gcttgcttga aaatattgtc gagacttcta aaggaactta gagtcactag aaaaataagg
     2581 agtagacttg atctatcaac aattcccttc ggggagtgac ggggtaggtt ttaacgattg
     2641 gtaacgtaaa aaattggtca atcgagtaaa tgcgcttgtt tggatagtgg tgaaatgaat
     2701 ttgtgtaaat aataagcgca gagtaaacaa gagggaattt agaagttaga caagttagaa
     2761 tagcgtctag attaatcatg agcagctact aagggatcta gactttgacg caaactcatg
     2821 gaataaggag taggtcttct cgtaatgggg aggacgcaca agaaattgtg tcgtgtgctg
     2881 cagtacatgt agatacttgc gaggacgttg attattggat caatcgagca tttaccgctg
     2941 tctactaggg tttctcgttt cggacgccat aaacgagtcg aaatttttta tgtattttta
     3001 agtaaacgaa aaacgataaa atatgaacaa gtcctgaata aagtgaaaaa attgatataa
     3061 aacatagaga aaatgaaatt tacgtaatgg aggaatgaca aatgaaaaat tatgatccga
     3121 atatacgatt gggcacacat actattaagg tttctttcca acgttgggat tacaaaggat
     3181 ttgtgacgtt tagaagaggc ggaaattgta aaggattaga tgttttagca ttagacgagg
     3241 atgatttata cgatcaaaca ttgactgata atccaattgg attcggcttg ctacctgaag
     3301 atgatgaagg taatgaatgg tttaaaatga cgcttatgaa tgataatgga gacgaattat
     3361 ctgttgaaga tacttggtca tatttgagtg attatattgt cagctttgaa atcattgaat
     3421 ttgtagcgga taaagaagaa taaaaactgg aggaataaat tttacagata aaggcttaga
     3481 aggagtcacg atgattaata aaaaggtagt attaccttag gcattgagta aggaat
//
LOCUS       DACDMM010000207         3504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDMM010000207 DACDMM010000000
VERSION     DACDMM010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.210"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            253..1035
                     /gene="rpsB"
                     /locus_tag="IWD16_002592"
     CDS             253..1035
                     /gene="rpsB"
                     /locus_tag="IWD16_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP9089575.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            1134..2015
                     /locus_tag="IWD16_002593"
     CDS             1134..2015
                     /locus_tag="IWD16_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP9089576.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            2151..2873
                     /locus_tag="IWD16_002594"
     CDS             2151..2873
                     /locus_tag="IWD16_002594"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP9089577.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            2876..3433
                     /gene="frr"
                     /locus_tag="IWD16_002595"
     CDS             2876..3433
                     /gene="frr"
                     /locus_tag="IWD16_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP9089578.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
BASE COUNT         1281 a          607 c          773 g          843 t
ORIGIN      
        1 taataaagaa acaaagagaa atctactgtt tttcttgaat tctttttatc tttcaggtaa
       61 actattgaat gttgagcaga aaagtatatt tgctcttgcg atacggatct tttgtctgta
      121 taatagctga gggtgcttta tgcatcagca aatccacatt cttattggat tgtcggtagg
      181 tgcttgaaaa agtttgccgg caaggtagaa gataagaaga ggaaaaataa aaccaaatcg
      241 gaggaaacaa acatggcagt aatttctatg aaacaattac tagaagccgg cgtacacttt
      301 ggtcaccaaa ctcgtcgctg gaatccaaaa atgaagaaat atatcttcac agaaagaaac
      361 ggaatctaca tcattgactt gcaaaaaaca gttaaattag tagatgcagc ttatgattac
      421 atgaagagcg ttgctgaaga aggcggcgta gcattattcg taggtacaaa aaaacaagca
      481 caagaagcaa tcaaagaaga agcaactcgt gcaggacaat acttcgttaa ccatcgttgg
      541 ttaggcggaa cattgacaaa ctgggatact atccaaaaac gtatcagccg tttgaaacaa
      601 atcaatgcaa tggaagaaga tggaactttt gaagttctac ctaaaaaaga agttgctggt
      661 ttgaacaaag aacgtgaacg tcttgaaaaa ttcttaggcg gtatcgctga tatgccaaga
      721 atcccagatg taatgtacat cgttgaccca cgtaaagaac gtattgctgt tcaagaagca
      781 cacaaattaa atatcccaat cgtagctatg gttgatacaa actgcgatcc agatgaaatc
      841 gacgtagtaa tcccttcaaa cgacgatgca attcgtgccg ttaaattgat tacttcaaaa
      901 atggctgatg ctttcatcga aggaaaccaa ggtgaagatc aagtagttga agaagatttc
      961 acagaagaaa acacagcaac atcaatcgaa gaaatcgtag atgttgttga aggcgacaat
     1021 tcttcagcag aataattttg aatgatggag ctgtttcaaa ggctgggcga caagcctcac
     1081 tctcctttga agcagctttt tttaaaaaga aaaacacaca ggaggagtta aacatggcag
     1141 acgttacagc taaaatggta aaagaactac gcgaaatgac tggcgtagga atgatggatg
     1201 cgaaaaaagc attagttgaa gtagaaggcg acatggaaaa agcagttgat cttttacgtg
     1261 aaaaaggaat ggcaaaggct gcgaagaaaa atgaccgcat tgcagctgaa ggattggctt
     1321 cagttgctgt gaaaggtaac actgcagcaa tcgttgaagt aaactcagaa acagactttg
     1381 tttctaaaaa cgaaatgttc caagatttag taaaagaaat tgcagaactt gttgctgaaa
     1441 acaaaccagc agacatggaa gctgcaatga aaatcaaaac agataaaggt acaattgaat
     1501 ctgacttgat cgaagcaact caagttatcg gagaaaaaat cagcttccgt cgctttgaag
     1561 ttgtagaaaa agacgacaat gctgcatttg gtggatactt gcatatgggc ggacgtattg
     1621 ccgtattgac tgttctagat ggaacaactg acgaaacagt tgcaagagac gtagctatgc
     1681 acgtagcagc tatcaatcct cgttatgtaa acgaatcaca aattccagaa gctgaattag
     1741 aacatgaaaa aactgttctt acagaacaag cattgaacga aggcaaacca gcgaatatcg
     1801 ttgaaaaaat ggtagaagga cgcttgaaga aattcaaagc agaaatcgct ttggtggacc
     1861 aaccattcgt taaagatcct gatatgactg ttgaaaaata cgttgcatca aaaggcgcaa
     1921 ctgttaaaac attcgtacgt ttcgaagttg gcgaaggtat cgaaaaacgc gaagataact
     1981 ttgtagaaga agttatgagc caagtgaaaa aataattctg ttgaataaca gacgggaacg
     2041 cacggtttgt gcgctccctt tttttaagca aaacaagaaa aaccagtctt agagtgtcga
     2101 aatcatctaa gtatggtaaa atggcaatag aaaatattgg aggaatgaac atggtcacac
     2161 cgaaatatca gcgtgtagtc ttaaaattga gtggagaagc tttagctggg gacgcaggat
     2221 ttggaatcaa accaccagta atcaaagaaa tcatccaaga aatcaaagaa gttcacgagc
     2281 tgggcgttga aatggcaatc gttgtgggcg gcggaaatat ctggcgcgga caaatcggag
     2341 cacaaatggg catggaacgt gctcaagctg attacatggg tatgttagct acagttatga
     2401 atgccttagc tttacaggat acgttggaaa acgtgggtgt accgacacgc gtgcaaactt
     2461 cgattgaaat gcgtcaaatc gcagagccat atattcgccg taaagcagaa cgtcatttag
     2521 aaaaaggacg gatcgttatc tttgcaggcg gtaccggcaa cccgtacttc tcgacagaca
     2581 caacagcagc attacgcgcg gcagaaatcg gcgcagatgt tatcctgatg gctaaaaaca
     2641 atgtagacgg tgtttattct gctgatccga aagtagatgc aaatgcagta aaatttgaag
     2701 aactgactca tctggaagtt attgccaaag gattacaagt catggattca actgcaagtt
     2761 cactaagcat ggataatgat attccactgc ttgtcttcaa tttaaatgaa cacggaaata
     2821 ttcgccgtgc aatcttaggc gaaaatatcg gaacaactgt aagggggaaa taaaaatggc
     2881 agatgcaatt atgacagaag caaaagaaaa aatgaaaaaa gcagcacaaa atcttcaacg
     2941 tgaacttggg caaatccgcg caggccgtgc gaacgctagc cttttggacc gtattactgt
     3001 gaactactac ggtgcgccaa caccattgaa ccaaatggca tcaatccaaa ttccagaagc
     3061 acgtgtttta atgatcacac cttttgacaa gagcattctt caagatgttg aaaaagctat
     3121 tatggcaagt gatatcggaa tcagtccaac caatgatgga aatgtcattc gcttagttat
     3181 cccgcaactg acagaagaac gcagaaaaga attagctaaa gatgttaaaa aagaagcaga
     3241 aaatgcaaaa atcgcgattc gtaacatccg tcgtgatgcg atcgatgaat ataaaaaaca
     3301 acaaaaaaat ggtgatatta ccgaagatga tttgcgcgga ttagaaaaag acgtacaaac
     3361 attgacagat aacagcatca aagaagtaga taagatcgct gctgataaag aaaaagaatt
     3421 attggaagtt taataaaatc ataaaaacag aggggcggat cattccgctt cctctgtttt
     3481 tatttatatt tcttttattt aaaa
//
LOCUS       DACDMM010000208         3495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDMM010000208 DACDMM010000000
VERSION     DACDMM010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.211"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            132..204
                     /locus_tag="IWD16_002596"
     tRNA            132..204
                     /locus_tag="IWD16_002596"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:165..167,aa:Lys,seq:ctt)
     gene            366..587
                     /locus_tag="IWD16_002597"
     CDS             366..587
                     /locus_tag="IWD16_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089579.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(911..1156)
                     /locus_tag="IWD16_002598"
     CDS             complement(911..1156)
                     /locus_tag="IWD16_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9089580.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(1150..1551)
                     /locus_tag="IWD16_002599"
     CDS             complement(1150..1551)
                     /locus_tag="IWD16_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9089581.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1723..>3495)
                     /gene="uidA"
                     /locus_tag="IWD16_002600"
     CDS             complement(1723..>3495)
                     /gene="uidA"
                     /locus_tag="IWD16_002600"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP9089582.1"
                     /translation="IDLSGLWRFKLDTNDDLQEELALEPMVEKESYPISVPSSYNDLF
                     ETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNGKLINQHKGGFTP
                     FESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKKENLVNFDFFNYA
                     GINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILIAIYDEEAQLIAE
                     AKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGIRSIQVSNGKFLI
                     NNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYSEEIMRLA
                     DREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKMAIKELIERDKNH
                     PSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPDICKVGDI
                     VDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTEYGADTVAGLHDT
                     VPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILRVQGNKKGIFTRD
                     RKPKMIAHYLKKRWSSIPNFSYKK"
BASE COUNT         1095 a          646 c          475 g         1279 t
ORIGIN      
        1 attttcaata tcgatggata actttcaaat attttttcat aatcttgaaa atccataaaa
       61 ttcactctca attctgataa taagtattgc acttttttat tttaggtgtt agaataacaa
      121 agtattaatt tgggctgtta gctcagttgg tagagcagat gactcttaat catcgggtcg
      181 cgggttcgag cccctcacag cccattgggt gccaaaccct taaatacagt gtaaacgttg
      241 gtgcttagca tttagaaaac gtatatactg tatttttttg ttttcaaaag agactgtagt
      301 accccttttt ttcactaaaa gcccactgta gtcattgata tttttgataa gaaaggaaca
      361 taattatgcg taatgaaatt gctaatataa caataatccg agaaacttta caaaaccata
      421 ctgcaaatga gatctctaaa catacaggat taaatttgag tactataaaa aaactaaaaa
      481 gtggagaacg ttcaatagaa aaactaaatt tacatgatgc tatttgcctc actgaatttg
      541 gtcttaaaaa taatagaaaa aatgttgaaa tcaacatatg gaaataattc ttaatcagct
      601 gcttttgtca aaagcacata ggaatttcac ctctctccaa gtttacggcg agccttacgg
      661 aagtatcatt atcccaactt caaatcatcg ctttttattg actataaaaa ttattttaga
      721 atgtttatcg ctccttcttt acagtaagaa atcatggcgc aactaaaata tgctccttga
      781 agtgttaacg tactgataga atgtatttaa atttcaatga tcaataggtt ttctgtttaa
      841 aagaatctac taatccttta actattagaa gattctttta aacagaataa gtggaaaggg
      901 gactttttag ttagttaatt ttaaatttta agatcgcttc tatcaattta atttctagct
      961 gtcttagcat atactcatct acataggtaa taaattgccc cgcctcatta aatccatttg
     1021 tcaaacataa cttagctata taactatgat aatgtttgag tatcatcttg attgaaattg
     1081 gatcttcttc tagagcagat tggataatgg atagaggaac taatggatac tgccgttttt
     1141 tggggttgtc taccataagt cataccccat ttttaattgt tcaactgcct tctttcgttg
     1201 ataccagata ccacttgtac tcatattgag gattttaccg atttcagtat cattaaaacc
     1261 tgcaaaataa taaagtagga taacctttct tcttatttcc gataaatgat caatggctgc
     1321 cgataacttt tgatctgaaa ttaatatttc cattccaaat aagaaataga catcttgttc
     1381 atcaaaatga gtttgggagt acttaccttc atttgaattc gtcaatggtt cctcattatc
     1441 tgttatgtta atgtttcgtt ttcgaataga cttagtttcg tttcgtaata cttttttaca
     1501 gtaggcatca aattgatgtt gtatagattt ctcattgtaa ttttgttgca tgataaatat
     1561 gctccttttt tcccctttcc actaatacaa caatcagaaa gcgccttttt ttcgaaaaaa
     1621 ctagaaaaaa atatccttct ttaaacagaa aatcttttta taaaaaaaag gagttggcaa
     1681 ttaagcagcc actccttttt tatattcctt ataaaaggta aattattttt tataactgaa
     1741 atttggtatt gatgaccatc gtttctttag atagtgagca atcattttag gctttctatc
     1801 tctagtaaag attcctttct tatttccttg cactcttaaa atcccttgac ttgtttcaaa
     1861 atctgcaaaa ttccacactt gttcaccaac aaaattgtca catttatcta ttacttcgtg
     1921 atttgcttcg taataaccca cttgatactc ttctgtaaac attgttggta ctgtatcatg
     1981 aagtccagct actgtatctg ctccatattc tgtaaacatt attggcgtat cgggacatct
     2041 ttccatccat ccatctagct cttttttcaa cagatatttt gcatgtttta ggtctccacc
     2101 tgaaacatac caaccataat agcgatttaa acataaaaca tcgacaatat ctcctacttt
     2161 acaaatatct ggtgttgact ttaagtaagt aacgattgtt acaggtctct tctgagggtc
     2221 aagttcttta gtcaattcaa ctaaaggttc aaaatattct ttagcgcctt cagaatccga
     2281 atctggttca tttgcaatag accacattac gacactcgga tggttcttgt cgcgttctat
     2341 caattcctta atcgccattt tgtgagcatt ttctgtccgt atttctttcc aagtatcatg
     2401 tttttctacg ttgtcatcta ataaagtagc catgaaattc aaatgtaatc ctactgcagg
     2461 tacttcatca ataactacaa taccttctcg atctgctaag cgcataattt cttctgaata
     2521 aggataatgt gaagtcctaa atgaattagc accgatccat ttcattaaat taaaatcttt
     2581 gatatttact gcttcattca aaccacgacc attaatatac gtgtcctcat gcttcccaaa
     2641 tcccttaaag taaaaaggtt tattattaat caaaaacttc ccattcgaaa cctgaatact
     2701 acgaatgcca aattcttctg tgtacgtatc aatcacttta ttttcgttaa ttaaactaac
     2761 tttcatacta tataagtaag catttagagg ctcccaaagt gttgggttct taagttgaat
     2821 cactccttct ttccccttag cttctgctat aagttgagct tcttcgtcat agattgctat
     2881 tagaatttct ccggtccatt ctgtatcgat attaaattct acatttgcat ctgaatcaat
     2941 tatttttgta ttaatagtga catcctttat atagtctttc ggagttgtat acaatttaac
     3001 gggtctatta atgcctgcat aattgaaaaa gtcaaaatta accaaatttt ctttttttat
     3061 gtctccatct tcctgtatcg tttctttcaa aatccctaca ggtaaagtgc tatcgtcaat
     3121 aacattgttt acagccactg taattctgtt acttccatct tttataaact gattaatttc
     3181 tgattcaaat ggcgtaaacc ctcctttgtg ttgattaatc aattttccat tcaaaaatac
     3241 ttttgcttca tgtgttgcag atccaaaacg aagaactaat ctttcttcta ataatttttt
     3301 aggaagaatc acttcttttt cataccacac ccatccaaca tgatttctaa tgttctctgt
     3361 ttcaaataaa tcattataag aagaaggaac tgaaattgga taactctctt tttcaaccat
     3421 tggttctaaa gcaagttctt cttgtaagtc atcatttgta tctaacttaa atctccaaag
     3481 accgcttaaa tcaat
//
LOCUS       DACDMM010000209         3424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDMM010000209 DACDMM010000000
VERSION     DACDMM010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.212"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            277..1062
                     /locus_tag="IWD16_002601"
     CDS             277..1062
                     /locus_tag="IWD16_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9089583.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1075..1926
                     /locus_tag="IWD16_002602"
     CDS             1075..1926
                     /locus_tag="IWD16_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP9089584.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2050..2502)
                     /locus_tag="IWD16_002603"
     CDS             complement(2050..2502)
                     /locus_tag="IWD16_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9089585.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(2967..3335)
                     /locus_tag="IWD16_002604"
     CDS             complement(2967..3335)
                     /locus_tag="IWD16_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP9089586.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
BASE COUNT         1085 a          552 c          690 g         1097 t
ORIGIN      
        1 aggggctgtg acaaaagtct tgtcacagcc tttttttcca aataaatggt ttttcagaag
       61 tagcttcttc ggaaataagc cgaaatttct tgaagctgac cacttctgtc acaacctctt
      121 tctttataga aataaaagga cactagcaat tcatgaaaac gattgctaga gcgtatcatt
      181 tgctttagac tatatatctt ggtgtacact cactttaagg cggaaataaa agttatccgt
      241 aataacaggt ttaggtatga aaaaggagtg ggtactatgt acaaggaatt agaaaacaaa
      301 gtcgcagtag taactggtgg ctcaaagggg attggaacag cgattgctca acgattcggg
      361 aaagaaaaaa tgaatgtagt tgtcaactat aactcggata aagaaggagc agaaaaagca
      421 gtcaaagcaa tcgaagaggc aggcggaaaa gcagtctctg tacaagcaga tgtcagttct
      481 gaagaaggaa tacagcgttt acttgatacg gctttagaaa attttgggac actggacgtt
      541 tgggttaata atgctgggat ggaaaatcag catccaaccc atgaacttag ccttgaagat
      601 tgggaaagag tgttaaaagt caatttgact ggcgttttta tcggaacgaa agcggcttta
      661 aattacttta cagagaataa taaaaaagga aatatcatca atatgtcttc tgttcatgaa
      721 cagatcccat ggccaacgtt tgctcattac gctgcttcaa aaggcggggt aaaattattt
      781 accgaaacgg tagcaatgga gtatgcaccg aagggaatca gaatcaatag tataggccct
      841 ggtgctatcc gtacacctat taatgcggca aaatttgatg atcctgaaca aaaagaactc
      901 ttggaaagta tgatcccact aggacgcgta ggtgaaccaa aagaaatagc ggcgacagct
      961 gcatggttgg cttcagacga atccagttat gttacaggta ttacactatt tgcagatggt
     1021 gggatgactt tatatccttc tttccaaggt ggaaaaggat aagggtgaat aaaaatgggt
     1081 ttattgattg cattgatccc ggcagttggc tgggggatcc agccactgat tttgaaaaag
     1141 attggtggca gaccaacaaa tgagatatta ggaactggta ttggagcggt gcttgtcggc
     1201 ttactcgttc aattattcgc ttctccagct ggtatttcag tgactacatt tttgattagt
     1261 ttactttctg gtgcattttg ggttattgga caaacaggac agtatacatc tttcaacttg
     1321 attggtgttt ctaaaacgat gccaatttcg actgcattac agttagtcgg aacctcgttg
     1381 attggtgtct tcgcttttgg agaatggtct ggaagtatgg ggaaaatcat tggagcagca
     1441 gcaatcattc ttcttgtatt tggttctgct ttaacggcaa tcagtgatga tcctggaaaa
     1501 aaaggcggta tcacaaaagg aattagtcta ttagcatcaa caagtattgg ttattgggta
     1561 tacagtgcat tgcctaaact agttgatgca agcggacttt ccattttctt tccgcaaatg
     1621 atcggtgtct ttttaggggc agtgatttat gtgattttcc gtaaaccaac cgcattttct
     1681 gaagacaaga gttggaaagc aacgatcgtt ggagccatct ttagtgtgtc agcattggct
     1741 tatatttttt cagcaaatga aaatggggtt gctactgcat atatcattac acagctaaac
     1801 gtagttatct ctacattagg cggtatgtac atcctgcatg aaaataagac accaaaagaa
     1861 aaaaaattaa cgattatcgg attattgctg atcgtagtag gaagcatggt cacggtcttt
     1921 atttgaggat aaagatggta aaatttcatt tattaaaaaa atatttgttt gaaccaaaca
     1981 gtaagtactt ataaaaaaat agcgctagaa tttccaacat ctctggaatt tctagcgctt
     2041 tttaattgtt taagatacaa gggaaatatc tgtctggatc aataattttt cattgatcca
     2101 ttgactaaag tttacgacat aattgaagtt accaccgatt agtatctccg catgattgtc
     2161 tttataaaga tcgtattgta aaagacggtc ttttttattt gcgtaaacat cgttatggta
     2221 ctggactaat tgttctcgga attgattgat tgctgtaaaa tcacgatatt gaaggttgaa
     2281 acgcatcgtg attggtgcat ttagcatgaa tcgctgcatt tcttttgctg cttcctctgc
     2341 ttgatctccg tataggaact caaaaagatt acgttcagaa aatttccatt gcccgccaac
     2401 ctttgttcct tttagttttc cttcatgaag ataatttctg atagaacgtg tagtaacacc
     2461 taattgttcg gctaattcat ttactgagta aaaatctttc aatgttcacc ctcctatgtg
     2521 aatggttcta ctgacttagt tgatatttgc ttaatcacga aatgtagaaa gaacgatttt
     2581 aattaaggtc attgtaaaaa tcaagtcaga aaattctaac ttttatttct ctaaagaaat
     2641 tcgttctctc ctaaagtgat attatattta aatatacctg aaaataatgg gcagtattat
     2701 gaaagatcac tgatattttc tcgtattttt tccttattca aaagttttgc aggaaaattg
     2761 ctgaaaaaat aattttacta tgtagaaaat tcaaagccat tcaatcagtg atttttttca
     2821 gcacaagaaa gactccttat aaaaatagca gatactttgt caattaagtg tttaaccagg
     2881 gatgataaaa aggataggat tgaacaaaac ctgtataagg tgatgaatag tttcctcgcc
     2941 agtaaaaggt ttattcgtat aaagctttat cgccaaaatt tatcatgacc ttttttatct
     3001 tcaatattaa atgaaatcaa ttgttcaatc aattcccagt tgattacact tttccaagga
     3061 actttgatga cgttatccgt atatacgtag cctgcttctt ggatattttt ttcaagggca
     3121 cgaatcgctg ctttttccgg tgcgatagaa aaatgatttt tagcaaaact aaatgcaata
     3181 ataaaggtac catggtcagt aaacataggt tggttccatt ttacagttgt gtctagctgg
     3241 ggatacgttt ctgttatctt agcaaaaagg tctttgatct tttgttgatg ttctttttct
     3301 ttgatttgat tgatgatcgg ctgaaatttt tccattctaa aacgcctcca ttaagtggtt
     3361 tgatttattt taccagaaaa acaatagtta tgtataaaaa acgaacaaaa cacaagtaat
     3421 gttg
//
LOCUS       DACDMM010000210         3415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDMM010000210 DACDMM010000000
VERSION     DACDMM010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.213"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..370
                     /locus_tag="IWD16_002605"
     CDS             <1..370
                     /locus_tag="IWD16_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089587.1"
                     /translation="SNAQIKDQRVAEHNNKKMSLTKNIYSMSDEELYKEFGDLSGEQL
                     EESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDVNTDSNAISIKDLKEL
                     LNSKRNELKQIKETNSVELE"
     gene            384..1073
                     /locus_tag="IWD16_002606"
     CDS             384..1073
                     /locus_tag="IWD16_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089588.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1198..>3415
                     /locus_tag="IWD16_002607"
     CDS             1198..>3415
                     /locus_tag="IWD16_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP9089589.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFF"
BASE COUNT         1153 a          457 c          724 g         1081 t
ORIGIN      
        1 ttcaaacgct caaataaaag atcaaagagt agctgaacat aataataaaa aaatgagttt
       61 gaccaaaaat atctattcta tgtcagatga agaattgtat aaagaatttg gtgatttatc
      121 cggggaacag ttagaagaaa gtattccagg aattagtgac tcagataaaa ttgttgtctt
      181 gtctgctggt gggcaattaa tgggagatgg caaatccgaa ttagttagtt ataaacaaaa
      241 tcacagacag tttattgatg ttaatactga ttccaacgct atttcgatta aggatctaaa
      301 agagctttta aatagtaaaa gaaatgaatt aaagcagata aaagaaacta attcagtgga
      361 attagagtaa aggaagattc attatgaaaa agatttttgg tgtttctgtt gggctactta
      421 tgatgttaac atttggaaat gcagtatatg ctgctgagaa agaagttatt tcagatccag
      481 agcagtacgc ctcacgatac tttgctgatt ttgaagaaga agataaagta cttgcattgc
      541 cagaaggtgg ttttttaaaa ggggaagcta aaattgtatc tatggataat ccagatataa
      601 ttcaaggttc atataattca gaaactgatc ctgcttcagt ttcagtgaca gaagcaaaaa
      661 aaattgttgc tgaaaaattc aaaaatgata ttgaaactac tccaattatg cctcgaggag
      721 catttccacc cgatgttggt actagcggac accttcttgt actagcagga ggaggaagct
      781 ataaatcaca gccatttagt ggtgcgggat ggcgttttgg aggtatgggt gtaaaaccag
      841 ctgatggtac aggcaacttt ttacgttgga aatcctacat tgacgatgga agagtaggaa
      901 atgtaaatca agccaacgca actaaatctg gttctattca aggaatggca ttgtatgctc
      961 gtgaaggatt caaatacttt gcaccaaacg ggcctggttg gacaatgaat ataggtattt
     1021 attacactta taatcctgtt gcaggtacgt attatgttgt tcaaaatatt taataaaaca
     1081 gcacaaagag atttctttgt gctgttttat tatttttagt tgttgacaaa cactaaaata
     1141 taaaatataa tcaacacata aacgaacgta tgttcggcaa gaaagcaggg gatttttatg
     1201 gatgagatgg ttttaagtac tcagaaatgg ctaaacaaga aatacagtaa tgttacaggg
     1261 tttgataagg ttccagaaaa tgggcgaaca gggtggccta caatctatgg acttattgaa
     1321 ggactacaag tagaacttgg tattactaat ctagtagcta attttggacc taccactgaa
     1381 aaaatgtacg ataatcaggt tactcctaaa tggggcaaaa atctacctaa aaatattgta
     1441 tttcttattc aaggtgcctt ttggtgtaag gggattaatc caggtggctt cgatggggta
     1501 tatactccat atttagatac tgctgtaaaa gagttgcaga ctgatgcagg gtttaatgga
     1561 agcgctgtaa ctggagtttt ggatgcgaaa tgggccaaag cgttatttga tatgagtgct
     1621 tttgttcttg ttcccggcgg agatagtaaa attagaaaaa tgcagcaatg gttaaatgta
     1681 aattatttag aatacacggg aataatgcct tgtgatggta tttatcaaag aaattcaaat
     1741 caagcattaa tttttgcatt acaagctgaa ctaggatatt ctcccagtga agcaacaggg
     1801 tcttatggta atgggacaac tagtaaaact tatcctgtgt ctgaaggtaa ctcgggaaac
     1861 tatgtaagaa taattcaata tggattatat gttaatggtt attttgaaga cggtacattc
     1921 gatggtattt tcactaaata tatgggttta gaagtactgg cattccgaaa atttatggta
     1981 ttgcctgaat atactgaaat agcagatgtt gttgtaataa aaggactatt gtctagcgct
     2041 ggagatatta gaagatctgc agatggcgca gatacttcta cgcaattaac tagatctcaa
     2101 atccaaacat tggttgataa tgatataaaa atagttggta gatatcttac tggtactgtt
     2161 ggaattggaa aagatgaacg aaataagtat ctaactacag aggaattgaa taatattttt
     2221 tctaaaaatt tatctgtgtt tcctatctat caagatggag gtgcagcatt agcttatttt
     2281 acttatgatc gtggcttatc agatggtaag aaggctatag atgcagcaaa aaatctaaat
     2341 ataccattaa caacagttat ttattttgct gttgatctag acatgttagg ggaagaaatt
     2401 cttgcatatg ctggagaata ttttaagggg gtatctgctg taatgtcata tagcgggtat
     2461 caaacgggtg tttacggaac aagaaatgtt tgttctcaaa taatcaataa cgggtatgct
     2521 tcgtttgcat ttgtttcaga tatgtcgact ggttatagtg ggaatttagg ttttcctatg
     2581 cctagacaat ggtcttttga tcaatttgtt gaatttacca taggaagtgg caacggagct
     2641 gttggtatcg atttaatcgc tacatcgggt agagatagcg gatttaatga gttatcaaat
     2701 gatactaata atgataatta cattgccaaa tacaatcaga aggttataaa tcagatggta
     2761 cgtgcgtatc aatatctatc acaagctggt cttgataatc cttggaaccc tcatttgaca
     2821 ttttatagat atgtaaatta ttctggtttg tcatgggaca tcatttcttc tcctgtaaca
     2881 gagcatgata gaaaaattta tgatgaatat aggaataaat taactaatag cgagggcctg
     2941 tataattatt ttatagatcc taatactggt tcaataatag gtttgcctca cttaattgtg
     3001 acgttacaat cacagatgtt tctagccgac acaatcaatg atagtgtttc tgactttgca
     3061 ggttggttag gagatttaat gacttgctgg ggtgaagtta agaaattagg aatacaaatg
     3121 gagcaaggtg tatttgcatt agtaggtacg gctgcaggtg gaacatttag tctagaagat
     3181 ttataccaag atgttgacgc ggtaaatgtt tataccttag caaaaagaaa ttctgattca
     3241 acagctttat cggctttaaa aacgtactat tgtgataagg gtgctattga tagatttaat
     3301 caatttgtta agaacagatt tggttcttca gataaaatcg cttcagtaac aaatagttta
     3361 ttagaaaatg acgaaacagt tgatatctta ggtgtaggga taatttcatt tttca
//
LOCUS       DACDMM010000211         3353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDMM010000211 DACDMM010000000
VERSION     DACDMM010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.214"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            372..1811
                     /locus_tag="IWD16_002608"
     CDS             372..1811
                     /locus_tag="IWD16_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9089590.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2106..2324
                     /locus_tag="IWD16_002609"
                     /pseudo
     CDS             2106..2324
                     /locus_tag="IWD16_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2573..2788
                     /locus_tag="IWD16_002610"
     CDS             2573..2788
                     /locus_tag="IWD16_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089591.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3005..3261)
                     /locus_tag="IWD16_002611"
                     /pseudo
     CDS             complement(3005..3261)
                     /locus_tag="IWD16_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          991 a          577 c          713 g         1072 t
ORIGIN      
        1 tatggaattg aatatcatag caatgtattt gaaaagcaaa ttggaaaaca aataggtgca
       61 acaccataaa tatgaaaaaa ttgggaaaaa gtagaatgga aatttgctta cgtctcacga
      121 gatagctcac actattaatc tgaagaacaa tgaagtcgac ttttagaaat tgctagaaga
      181 aagtaaagcg aattatcact agttactata gagtaagttc aaataaagca tctccaactt
      241 acatggttac atgaggatat caaatcgaaa ttactcaagc ttcctatggc tagagtcgcg
      301 agaagttgtt gggcagtaga cataggttat attgttctta gctacacgat cattagaaag
      361 gaaatcctat tatgtatagt tatcaagaag atgcaaaagt ccaaaaaaat cgctggtgga
      421 ttttgattgc tatatctatg tttaccttca tgtccacatt ggatggcagt attgtcaata
      481 ttgcactacc taccatctca gatgatatgg gcataccaat gaatcaagcg gaatgggtag
      541 tgtccgttta tctgatgatt gtctgttctt gtttgttgct gtttggtaaa attggcgata
      601 gccgtgggaa aatcaaagta tttcggattg gtacgctgat ctttgtcttg ggatcgctgt
      661 tgtgtgggtt caatcattcg ctaggtattt tattgttcgc acgggtggta caagccattg
      721 gtgctagtat gactatggct acaaatgccg gaatcatcac ggaagtcttt cccatacatg
      781 aacgtggtcg ggcattggga gcaataggtg cgtttgtttc attaggctcg attgctggac
      841 caggtcttgg cggcttgatt ttgtcccaat tttcttgggc gtacattttt tggatcaatg
      901 taccggtggg aatcgtaacg atgttgattg ctgaaaaatt cttaccaaaa gatatcacca
      961 tgagcggcca tacaatcgat ggagggggct ttgctgccat tgcggctacg attatcagct
     1021 tttttggagc aatctttttg gggcaagaaa gtggcttttt ccatccagtc ccccttgtac
     1081 tgttcgtaat ttcagtgatt gcctttgtca gctttttaaa agttgaaaag aaagcagcct
     1141 ctccattgat ttctttcggt atttttaaga acaagatttt taccatgagc ttgattacag
     1201 ccttgttgat tttttcttcc aacttctttg tcaatgtagt gattcctttt tatctgcaaa
     1261 atgctcgcgg gttgagtcct agctatgctg ggctgttgat gatggtcttt cctttgatga
     1321 tggtcgtcgg ggcgccttta agcggctacc tgaccgaccg ggttggcccg gagattttgg
     1381 tattagtagg cttgggctta ttgtcggtaa cccaggtcat gtatatgttt ttgtcagtgg
     1441 actctccttt gtggctatac attgtcgcta ctgccatcat gggtgccggc aatgccttgt
     1501 tccagtcccc caataatacg atggttatga gctcggtcac tatcgaaaac ttgggactgg
     1561 caggctcgat gaattccttt gcccgaaatt tggggatggt cagcgggatt tcgctttcta
     1621 cgacggtctt atatcaagcg atgtctgcta agatgggcca gcaagtaact aactatatcg
     1681 caaatcggcc ggatgtcttt ttatatggaa tgaagattac ctttttaggc agctttgttt
     1741 tgtgttttgt tgcctttttc ctgactatct ggcgtatcaa aaaacaagga ggtattcaga
     1801 aaaatgtctg aaaaaaccac tgttgaagaa tttttggagg cctgcgtatg ggtgagtggg
     1861 atttccatgg atgatggaag atgaagtgaa cttctaaaag ccgaaaaact caagtattat
     1921 cttcaaaata ctagtgctca atgagatgct tgatttgatc acaagaagac tgcgaaagcc
     1981 gctggcaacc gcaagctagg tatcccaccg atggactacg ttttagaggc tactggttat
     2041 gaatacggag ccaacgctct catctgcata agaatacatc acccagatta tgtaaagatt
     2101 ttcgatactt ctgtcaaaaa atcattaaga tagataaatg atttccgtct agtcaaattg
     2161 gttcagaatg tagacacaca gatggtaaga agtcactcac aattcgagaa tggacttgtc
     2221 ctatttgccg tatacatcat aatcgaaacg tcaacgatag tatcaatatt ttgaccaagg
     2281 gctatgaata caagcattgc cttagaaaac atcaagaacc ttaggaacaa cggggatagc
     2341 ttggttaata agagataccg ctgtttaggc ttgcaagtat actcttttat caagaattcc
     2401 gtggctttag tcatgagagg cttaatacta tgaaaatttt ctttagcatt tttgatttag
     2461 gttccttttc taattataat aagatgacca ctacagtaac ctactgaaat atataaacag
     2521 ccactgtagt accattttca aagtagtcta tgagaggaga atcttcattc ttatgaacgc
     2581 tggaattgcg gatatgaatt taataaaaaa aacccttaat gattttactt ccaattcaat
     2641 atcaaaaggt actggtatca atttaagcac aataaaaaag ctgaaaagtg gtgaaagatc
     2701 agtggaaaag ctgaatcttt tagatgctat aaaaataacg gaatttgcta tgaaaaatgg
     2761 aaaagctgag attgaaatat ggagataatt atcgtctagt gaattacttg ccacttagct
     2821 gaatttaacg atccttggtg cttgaagggg gagattcttg ggaatagagc atacttgcaa
     2881 gcctatgtct ttactaacaa cgatatctct tattaaccaa gctatccccg tagttctaag
     2941 gcttttactt ttatctaagc aataatcgta ttcaaaggaa atttttgacc cttagaaagt
     3001 ctcattttat cagacgtgag cttaggcatt gctgaatttt gttaattcgg ttttaggttg
     3061 tgctttgagc ataatatgaa agtattcttt atcacgattt cattcatcta aggtaatgtg
     3121 atgtgattct gaaattcttt caaaggcagt ttttgcataa gctgacatta catcatcaat
     3181 gacatgttta caatgtttca ctactaaaat aagggggtaa ccaagaagaa aaactgaatg
     3241 attatttgta tctaattcca tggtatatca accatttcct atatagactg attatgacat
     3301 ggattaaaat aaaaaggcac tatgtcttaa ttgtcggttt tcgagttacc gaa
//
LOCUS       DACDMM010000212         3328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDMM010000212 DACDMM010000000
VERSION     DACDMM010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.215"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            76..333
                     /locus_tag="IWD16_002612"
     CDS             76..333
                     /locus_tag="IWD16_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP9089592.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            565..969
                     /locus_tag="IWD16_002613"
     CDS             565..969
                     /locus_tag="IWD16_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP9089593.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            988..>3328
                     /locus_tag="IWD16_002614"
     CDS             988..>3328
                     /locus_tag="IWD16_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9089594.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLM"
BASE COUNT         1215 a          445 c          623 g         1045 t
ORIGIN      
        1 tcagcaatcg aaggaaaata aaagaagaaa atggtacgca aaatctaact attagagcaa
       61 aaacaggaag ctgctttgga agatagtccc gaattgtttc cgatgttaaa agatcgttta
      121 aatgaattga ctgaaaaacg aagacagtta caaattcgag agaatgaaat cttagggatc
      181 tttcaacaac aaggggaacc gattcaagtc aaagatgtcc aacagatatt gactagtttg
      241 gattgctttc ttatccagag tgagaagaag caagttaaag ctttgtatca cacctttatt
      301 gagaaaatca cctttgatcc taatcagaag tagatattgc cgatcccgat gagattcaca
      361 cctactgtga tttgataatt gaatgagtaa tatcaaatag ccgtatccaa accaaaggat
      421 atggctattt ttgtgttagg gatatacgtc acgtagcttg ttatcaagaa taagtttctt
      481 tatttttttg ctttttctaa aactgagtat gctaaaataa gagggtatat agcaacttta
      541 aataagagat aggagtgtat ctttttgggt gcagaattaa atcagaagtt atttagtgcg
      601 gcagataatc tccgtagtaa aatggatgcg tcagaatata aaaattattt attggggctg
      661 attttctata aatatttatc cgaccgtttg ttggaacaag tggttctttt agcagatgaa
      721 tccttagagg aatatgacac agtaagcaaa caaacgatgt tgtatcgtga attactttca
      781 gatgaagaat caaaagaaga tctgattgca acaatcgttg atattttggg gtatgcgatt
      841 tcgccagagt atcttttcaa cgtcttagcg gatcaagcaa aacaagcaac gttccaattg
      901 aatgatctaa acaaagcctt tttagcaagt catatttatg gaaaaatact gtcggcaaaa
      961 ttcgaatagt gtcgaggagg tgctgtgatg gaagaattaa aagaacgcat tgtcaattat
     1021 gtcatagaga actctgagat taaaaataaa tgggggaaca atacagcaca gaaaatatct
     1081 gaaaaattaa atgttagtcg taatattgtc tctcaatatt tgaatgaatt cgtaaaagat
     1141 ggagaatttg gaaaaatcaa tactcgtcca gtaatcttct tttctttacc ggagaaagtt
     1201 ccaaatcaac aaatttttag ctcttcagag gaagcggaaa aatggtatga tagctctttt
     1261 agggttggaa tagaaagggt tatcggaagt caaggaagtc tgcgaaatgt aattgctcaa
     1321 attaaagcag caatctacta tccgccaaat ggcttaccca ttttacttca tggcgagact
     1381 ggaactggaa aaagctattt agcaaattat gtgtataagg atttgttggc caataattat
     1441 atagaagaga aggcatcttt catttcagtt aattgtagtg aatatgccaa taatccagaa
     1501 cttttcttgt ccaatttttt tgggcatgta aaaggtgcat atacaggagc cgaagatgga
     1561 agaaagggtt tagcagaatt agctaataat ggagtcttat ttttagatga aattcatgca
     1621 ttgagcaatg aatgtcagga gaaacttttt cagtttatgg atacgaggaa atttcatcgg
     1681 atgggtgata atgaaaactg gtacacatca agttgctgga ttatatttgc aacatcagaa
     1741 ccacctgagg ataaactatt atcaactttg ttgagaagaa tacctgtaaa gctaacactt
     1801 ccaacattag acagtcgttc tctttatgaa agaaaacagt taatcaagat attttttaaa
     1861 tcagaagaag aaagactagc aaagaaaata ctgatatctc aacaattatt agatttttta
     1921 ttaaactatc gctttagtga aaatgttggc aatttgaaaa atatcattca attgacctgt
     1981 gccaatgcct tttatttctt agatgatttg gaagaacttt atattcagat gtatcattta
     2041 ccacaatggg tgaatttcaa cggcatgaag gttatgaaaa gacagaatga attaaaatat
     2101 attcaaatgg atgatttgga aaaagtagac gaagttttta atatcaacga acttatagag
     2161 tatctttatc aaacgtggaa gaaagacact gataagcagc agctttttga gaaatatgat
     2221 agatatttta gacaaaagaa tgttgatatc caaaataatt cagctttaac atatcccacc
     2281 tatcaagaga ttcgatttag acagtatgtt aaggaaatag agagagattt tcaaatagaa
     2341 tttgactatt attatcttca aataacgtct atctttttga attcttggat ttatcaaatc
     2401 caattaacat cggacgataa attatattcc ttcatcaatg atattaatag ttggtgtagt
     2461 cgagaagttc aattagtcaa tcgttttatt caaatagggg atatttattt aacaaaaatt
     2521 caaaaaaatg ttttggttat tttatttaag ttaatgtgta gtaaagaatt tcagcaaaaa
     2581 cgtttagcac tgattttggc tcatggagat ctaacggcaa cctgtatagc aagtactgct
     2641 aataggatcg ttgagagata tatatacgac gccattgata tgccactaat gacttcaagt
     2701 agtgagattg cagacaaaat taatagatac ttacaagagt gtaccggata tgaagaaatt
     2761 attttacttg ttgatatggg ttcactggag aaaatttatc taaatttgga actagacgct
     2821 caaaaaaaat tcggactact aaacaatgtt tcaacaagaa tggctattga tgttgcaaat
     2881 cagatattat tagatttacc gcttaaacag attcttgaac aagtaaaaga aaatcaaaat
     2941 gtggattata tttatgaatc atcaaatatt aagaaggata tgattgtctg ttcttgtgca
     3001 agcggattag gaacttcgtt taaattaaaa aagatacttg aagatagctt tccaagggat
     3061 actgatcttg tcatagaaac gtgtgactat tactctttag tgaatgaaaa ctatttatcg
     3121 gagcttcgta aagagaacaa tattcttttt attgttggaa cactaaatcc acatctagaa
     3181 gatgtagaat tcttttcaat tgaagatatg attttaggaa tgaatataag taaattctcc
     3241 aatcaattga atagacactt taatgataat gaactgaaag ttttgaacaa taatattctg
     3301 aagaatttta gtctaacgaa tttaatgg
//
LOCUS       DACDMM010000213         3304 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDMM010000213 DACDMM010000000
VERSION     DACDMM010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.216"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            278..787
                     /locus_tag="IWD16_002615"
     CDS             278..787
                     /locus_tag="IWD16_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9089595.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IWD16_002616"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IWD16_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9089596.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IWD16_002617"
     CDS             2322..3302
                     /locus_tag="IWD16_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9089597.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
BASE COUNT         1180 a          529 c          653 g          942 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagt
//
LOCUS       DACDMM010000214         3184 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDMM010000214 DACDMM010000000
VERSION     DACDMM010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.217"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..430
                     /locus_tag="IWD16_002618"
     CDS             <1..430
                     /locus_tag="IWD16_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9089598.1"
                     /translation="PNDLVVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLIL
                     DQPEDDLDSQLINNLIVRSIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISA
                     DQCGSVDEPNIKPRICTVMEGGEDAFTNRAKRYGFKQIVE"
     gene            480..869
                     /locus_tag="IWD16_002619"
     CDS             480..869
                     /locus_tag="IWD16_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9089599.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            856..>3184
                     /locus_tag="IWD16_002620"
     CDS             856..>3184
                     /locus_tag="IWD16_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9089600.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1020 a          536 c          692 g          936 t
ORIGIN      
        1 gccaaatgat ttagtagtta tccgacttga actaaatgga aaaaaagtta gtttatcgaa
       61 tgctagtgct ggtcaaaaga catctgcaat tctttctatg attttggcgt atggaaattc
      121 accactcata ttggatcagc ctgaagatga tttggatagt caattaatta ataacttaat
      181 cgtaagaagt atcattacaa aaaaggaaaa cagacaaatt ttaattgtaa ctcataatgc
      241 aaatatccca gttaatggtg attcagaatg gttgattagc atgggagatt caaaagaaat
      301 atcggctgat cagtgcgggt cggtagatga acccaatatc aagccaagaa tatgtactgt
      361 gatggaaggt ggcgaggatg cttttacaaa tagagcgaaa cgctatggtt ttaaacaaat
      421 tgtagaataa cactagaggg tgagacatat tattatgtcc gcccttttta ggaggaaaaa
      481 tgaaaaaaat aaaaagctat accagtatct gggcagttga aaaagtcatt tacgcaatca
      541 atgattttca gttgcctttt cctgtgactt ttaaccaaat ggcttggttc gttttatctt
      601 tactgtttgt catcgtattt gcccatgtgc ctccactatc tatgatagag gaggtctttt
      661 taaaatactt aggcataccg gtggctgtca cttggtttat gtcgcaaaag acgtttgatg
      721 gcaagaaacc actgggattt ttacgatcct ttatcagcta tcacttgcga tttaaggtga
      781 cttttgcagg aaagaaagtc aaagaacaga agaaacgatg ggatgaaccc agtactcttg
      841 taaggagtgt gaactatgta tccgattaag tatattgaaa ataatctcgt ctttaaccaa
      901 gaaggcgagt gttttgccta ctatgaatta gtgccttaca attactcctt tttgtcaccc
      961 gaacagaaat ttcaagtgca tgataacttt cgccaactaa ttgcgcaaaa tcgtgaaggc
     1021 aagatccatg cgttacaaat tgccacagaa agtagtattc gcgccacaca agaacgatca
     1081 aaaaaagaaa ttaccggtag actcaaagaa gtagcgaaac aacgaattga tatacagaca
     1141 gatgcattag tatccatgat tggcgatagt cagattgatt atcgcttttt tattggcttt
     1201 aaactaattg cgacagatga agaagtcaat ctaaagagtc tcaagaaatc tttcttttct
     1261 gggcttcaag aatttgtcta tggagtgaat catcatttga tgggcgattt tgtttcctta
     1321 tccaatgaag aaattcgctg ctattctaag cttgaaaaat taatggaaag caaattggct
     1381 cgaagattta aagtcaggcg agtcacaccg agtgacctga cgtatttgat tgaacatatt
     1441 tatggcgaaa aagggatacc gtttgaagaa tacgaatttc agctaccaaa gaaaaagcta
     1501 aaatcagaaa cactggtgaa acgttatgac ttattacgtc caaatcgttg tttaattgaa
     1561 gaaaaaccaa ggtgcttacg gatggaacat gagaatcatg agtcgtacgt agcgtattta
     1621 acaattaata caattgtggg ggagatggaa tttccttcct cagaactttt ctattaccag
     1681 caacaacaat ttacattccc aatcgacaca agtatgaatg tagagattgt gacgaataaa
     1741 aaggctttag ctaccgttcg taataagaaa aaagaactga aagatttaga caatcatgct
     1801 taccaatctg acaatgaaac gaattccaat gtcttagatg ctttggattc cgtggatgaa
     1861 ttggaaacca ccttggatca gtcaaaagaa tctatgtaca aactgagtta tgtggttcga
     1921 gtgagtgccg aatcagtgga tgaactaaag cgacgctgtg atgaagtgct cgatttttat
     1981 gatgatacca atgtaaaact agtccggccc tttggcgata tgatggggct acatgaagaa
     2041 ttcttaccct caagtaaacg atatatgaac gactatattc agtatgttac gtctgatttt
     2101 ttggcggggc ttggttttgg ggctactcag atgctaggag aattagaagg gatttacttt
     2161 ggctacaatg tggatacagg tcgtaatgtt tacctgaaac cggcattggc ttcacaagga
     2221 gtcaagggtt cagtaaccaa tgcgctggcc gccgctttcc tgggttctct tggtggaggt
     2281 aaatccttta gtaacaatct cttagtctac tatgctgttt tgttcggtgg tcaagctgtg
     2341 attgtcgatc caaaagggga gcgaggaggg tggaaagaaa ccttgccaga aattgcggat
     2401 gaaatcagta tcctcaactt gacgagtaag tcagaaaatc aaggtctgct tgatccttat
     2461 gtgattatga agaaaaagaa agactcggaa agtttagcaa ttgatattct gactttttta
     2521 acaggaattt caagtcgaga cggggagaag tttcctgtgt tacgtaaagc cattcgaacg
     2581 gtgactcaaa gtgatcaacg ggggttatta caagttatta acgaattgcg aaaagacggg
     2641 tcaccaatcg cggaaaatat cgccgatcat atcgagtcct ttaccgatta tgattttgct
     2701 catttgcttt tctcagacgg ctcgattacc caatcgatta gtttagataa acagctaaat
     2761 atcattcaag tggcggattt ggttttaccg gatgcagaaa cgacttttga agaatatacc
     2821 accatggaat tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc cttggacttt
     2881 atccatagta accgagaaat cttcaaaatt gtcgatttgg atgaggcttg gagttttctt
     2941 caagtggctc aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg ggccatgaat
     3001 gctggggtct atttcgtcac tcaaaatagc gacgatttat tagatgaacg tttaaaaaat
     3061 aatatcgggt tgaagtttgc ttttcgcagt accgatattc atgaaatcaa gaaaaccttg
     3121 gaattctttg gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg agatttggaa
     3181 aacg
//
LOCUS       DACDMM010000215         3145 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDMM010000215 DACDMM010000000
VERSION     DACDMM010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.218"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(59..787)
                     /locus_tag="IWD16_002621"
     CDS             complement(59..787)
                     /locus_tag="IWD16_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9089601.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="IWD16_002622"
     CDS             complement(800..2080)
                     /locus_tag="IWD16_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9089602.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="IWD16_002623"
     CDS             complement(2221..2823)
                     /locus_tag="IWD16_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP9089603.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
BASE COUNT         1056 a          545 c          552 g          992 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aagga
//
LOCUS       DACDMM010000216         3127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDMM010000216 DACDMM010000000
VERSION     DACDMM010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.219"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            103..1347
                     /locus_tag="IWD16_002624"
                     /pseudo
     CDS             103..1347
                     /locus_tag="IWD16_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="IWD16_002625"
     CDS             1360..1992
                     /locus_tag="IWD16_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9089604.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="IWD16_002626"
     CDS             2197..2424
                     /locus_tag="IWD16_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP9089605.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="IWD16_002627"
     CDS             2493..2759
                     /locus_tag="IWD16_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9089606.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="IWD16_002628"
     CDS             2749..3018
                     /locus_tag="IWD16_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9089607.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          943 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gcttttt
//
LOCUS       DACDMM010000217         3112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDMM010000217 DACDMM010000000
VERSION     DACDMM010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.220"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            47..477
                     /locus_tag="IWD16_002629"
                     /pseudo
     CDS             47..477
                     /locus_tag="IWD16_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            616..1038
                     /locus_tag="IWD16_002630"
     CDS             616..1038
                     /locus_tag="IWD16_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089608.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            1183..1428
                     /locus_tag="IWD16_002631"
                     /pseudo
     CDS             1183..1428
                     /locus_tag="IWD16_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1537..2493
                     /locus_tag="IWD16_002632"
     CDS             1537..2493
                     /locus_tag="IWD16_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089609.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
BASE COUNT          976 a          517 c          687 g          932 t
ORIGIN      
        1 aatctttttt ttgctgtatt catttagatt atgatagaga aaatacgtga gagggggacc
       61 tgggatgtgg ctatctgatg aaaaaattca gtggcgtagc tatcagaagc gtatattgac
      121 taactttgct acttatttaa ataacaattc agtccatcta gtggctccac caggggctgg
      181 caagaccttt ttaggaattg agatgatccg acgtttggat tgtaaaacct taattttggt
      241 tccttcccta atattgaaga aacagtggtt aacaacaatc gaggaatatt tcttagtaaa
      301 tccagcagat agttgctatc tttctggggc gattgaacat cctagtcata ttacagtgga
      361 aacgtatcag accatttaca accgactgaa ggaggagcca acttatggtg atcagctgga
      421 gctgttagtt ttttgatgaa gctcatcatt taaaaaaaag ctggggtgaa ttgttgattg
      481 acttgaagaa aaaacaaccg aatctgttga cgatttcctt gacagccacg cctccgattg
      541 atagctctca gacggagtgg aagcaatata ttgagttaaa atggcctgat tgacgaagaa
      601 attagcattc ccgagttggt aaaggaggag gttctatgtc ctcatcagga ttttctatat
      661 ttgattccgg ctaatcagga aactactcag cattatttta cctttttaga gaaacaaaat
      721 gagctggtgg aagcgttaat ggaaagccaa gagacggtgg attatttgct ttctttagaa
      781 tttgttcagc atccgcttga gcattcgaag gaaatttatg aaaattttga gctgttcact
      841 agtgcattaa tttacttgaa tcatcataac tatgaattga tacccgaaca ttggcagcta
      901 ttaggcagtt ctaagcagca aggtaaaaaa gcttcagttc catctttagg taaaaaagag
      961 ctgatcacta tttatcagca gatgtttgaa gatcaaccgg aattgcctat tttttatcaa
     1021 ttgaaaaaag ctggatgatt gtttgatgag tcgctgcatt tgtttccaga gtatccagac
     1081 actgttggta gtcaggcgat tcctgagaaa aagcaagcag tttgtcaaat tttgttaaaa
     1141 gaagaagttc agctgaagca agaactgaca gcggtcattt tgatggatcg tatttgcaag
     1201 gaggtattag caggcagtga tggtacctta gcttatggcg tggttccttt gtttgatgaa
     1261 gttgttcgtc tattaggtaa cactcagcaa atcggtgcca tctgtggaga gttcttactg
     1321 ttaccgaaaa tgctagtaac tgttgagctg gcagaactag cgacgggtat gaatgaacat
     1381 ccttatatgt cgggttattt ttatttgacg gtggatgaca aaaaccgcca agcattgatg
     1441 caagcagtta ctcgcctatt aaccgaaaag aagttgaata ttttggtagg aaccgttagt
     1501 ttgctaggag aaggctggga ttgtcctgca attaatctgt tgatcttggc taatcaagct
     1561 tccacttttg tgcaaacaca acaactacga gggcggggct tacgcctagg tgcacaaaat
     1621 aaagtaacga acatttggca tttagcgatt ggattaccgg gagttcctct gtctgaacag
     1681 cctgatttga aaagaatgct acggcgtttg ggcaatattt caggtctgac ctttgaatct
     1741 tcaccaaaaa ttgagagtgg tggcgatcgc tttgctttac cactggaata tacgaccacc
     1801 acgatggaaa cttatactca gtcgatgttc tatttttctt gtgaacgaag tcggttgaaa
     1861 gaacaatgga gtactgcttt agctgatggc tggcacctat ctatgccaat cgtcgttagg
     1921 aaagtcgatc aggtaaagca aaaacaagaa acttcattgc cgatagtagt aaaaaatata
     1981 gagcctctaa gcttctggtc agctatattt aaaggtaact tgctggaata tttccaaatt
     2041 cgtaggcgac aaaagctatg ggaaaaggaa tgtgcccgca aaaaaagctt ggccatatgc
     2101 gttcaaacga cgttgcagcg ccttggtgaa gttagtgata gaaaagtaat cgtagcggct
     2161 gatcagcagg aatttagatg ctatcttgag aatgctggct atcgagaagg acagcttttt
     2221 actgaagcta tgagggagct tttagcaccg ctggagaatc cgcggtattt aatcgaaggg
     2281 aaaaagatag ttctgacggt accagaacgc tttggccgaa ataagcaaat cgctaacctt
     2341 tttctaaacg aattaaatca atcgtcgctg cccaaatgtc agttggtgta tactagaaat
     2401 cctgaaggac gccaaaagct agtgacttat cgaatgaggc aagtcttcca gacccgcgat
     2461 gaagtaatta ttcaaaaaaa catttggacg taagttgatg aaaataaatt ttgctagagt
     2521 agaacaagtc cgagaattaa gttgctactt aactgataat gccaaggaca ggagaatctt
     2581 ttcttgacga ctgaaagaaa ctatttgact tatataacgg tgtgtgatga atatttgcaa
     2641 ggcattctct taaaaagtac tcgtaatagt ttttttgaaa acaaaacagt actttattaa
     2701 taggactgac agagcgatat aagaacatct gcagacagag gtgaatgctt ttctcatgat
     2761 cgatgaaaca atggtacttc ggatattctt gcttttagtt tgaaccgttg tgattttctt
     2821 tgattcctaa aagtagtttt atagaatgaa aatcaagcat acggaatgct cctttgattg
     2881 tgttgtggtg attcaatcta atggagcttc tatatgtttt tttatttgca aaaagaaccg
     2941 atagaatatt tttatcagtc ttagaaatta tagagccaag aaatttcaag cacacataaa
     3001 aacgcgtgtt ttatgcattt tatgagcaaa aataactaaa gaccattacg taaaacatag
     3061 gtaaatcagc attcaatctc aaattatgtg atcaaatgtt gatattattt ga
//
LOCUS       DACDMM010000218         3057 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDMM010000218 DACDMM010000000
VERSION     DACDMM010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.221"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IWD16_002633"
     CDS             complement(14..706)
                     /locus_tag="IWD16_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9089610.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IWD16_002634"
                     /pseudo
     CDS             791..967
                     /locus_tag="IWD16_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IWD16_002635"
     CDS             complement(1143..1712)
                     /locus_tag="IWD16_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9089611.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IWD16_002636"
     CDS             complement(1786..2109)
                     /locus_tag="IWD16_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089612.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..>3057)
                     /locus_tag="IWD16_002637"
     CDS             complement(2263..>3057)
                     /locus_tag="IWD16_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089613.1"
                     /translation="LSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDRHLKRLA
                     ESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYEQKYDRS
                     TQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQSFSEMFI
                     QLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSCNQITEK
                     RWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
BASE COUNT          845 a          615 c          541 g         1056 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataa
//
LOCUS       DACDMM010000219         3013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDMM010000219 DACDMM010000000
VERSION     DACDMM010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.223"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            177..359
                     /locus_tag="IWD16_002638"
     CDS             177..359
                     /locus_tag="IWD16_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAP9089614.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="IWD16_002639"
     CDS             462..746
                     /locus_tag="IWD16_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089615.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="IWD16_002640"
     CDS             762..998
                     /locus_tag="IWD16_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089616.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="IWD16_002641"
     CDS             1020..1274
                     /locus_tag="IWD16_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089617.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="IWD16_002642"
     CDS             1356..1541
                     /locus_tag="IWD16_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP9089618.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="IWD16_002643"
     CDS             1608..2003
                     /locus_tag="IWD16_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9089619.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="IWD16_002644"
     CDS             1993..2370
                     /locus_tag="IWD16_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089620.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2371..2766
                     /locus_tag="IWD16_002645"
     CDS             2371..2766
                     /locus_tag="IWD16_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089621.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DACDMM010000220         2958 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDMM010000220 DACDMM010000000
VERSION     DACDMM010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.225"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1770)
                     /locus_tag="IWD16_002646"
     CDS             complement(<1..1770)
                     /locus_tag="IWD16_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9089622.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1724..>2958)
                     /locus_tag="IWD16_002647"
     CDS             complement(1724..>2958)
                     /locus_tag="IWD16_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9089623.1"
                     /translation="KMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
BASE COUNT          954 a          558 c          421 g         1025 t
ORIGIN      
        1 tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt tctttatgag
       61 atttttgtat atttctgacc ttcgatacaa ctcattatga gtaccttgat caactagttt
      121 tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc gatgcgatat
      181 aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac gtttttcatt
      241 ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat ttttcaaaaa
      301 agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc cgttttctcc
      361 gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt tagctaaacg
      421 agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta tattttccaa
      481 aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc taaacaaaga
      541 agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt catcataatc
      601 atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag cacctactaa
      661 cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg gggtattgtt
      721 atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag gactatctga
      781 tccctcttgg atactggttt ccttcatagc tctaattcgc tgtattttag gagctaaata
      841 aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt ttattgcggc
      901 tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta agtgaacacc
      961 tactagaatg actactgcta aactaacata tgagaaaata gaggataagg ccatgactga
     1021 aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt ttttgaaaag
     1081 tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca attctatagt
     1141 ttcttgaaat tcttccgagt tttctctcaa agtatcgtaa tatttagtat tggctttcgt
     1201 ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca aaataacagc
     1261 taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga aaaacagagc
     1321 cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt caacgtcaga
     1381 catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata atggtaaatt
     1441 tgacaaatcg tctgcaattt gcactcttaa atgcgcactt tctttataag tactattata
     1501 taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa caaacgatac
     1561 ggcaaaataa agccaacggg gctttgtatt ttctaaaact aattcgtcta tcagcatcat
     1621 tataatgaat gctggtacca tattaatgca gtatacaaaa aaactagaaa tagaagcttt
     1681 agttaaagct tctgcgccct gatcacttaa agcaaatttg tttttataaa aacttttcat
     1741 tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta tacacttgat
     1801 gtttagccat taattcatta tgatttccac gttctactat tttcccttct gacaagacaa
     1861 gaatttcatc tacagattta atgctggata atcgatgagc tatcataata actgtttttt
     1921 ctttcatcag atgttgaaaa gccttttgta gttcatactc attatctgga tcaatagatg
     1981 cactggcttc atccataata acaatctttg catcttttaa aattgctctg gcaatagcta
     2041 tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag gtatgctctc
     2101 tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg tcaaatactt
     2161 tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc tgattgaata
     2221 gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt gaatagtgat
     2281 ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa cctgaaatta
     2341 gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa attctttttt
     2401 gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa aagctgacat
     2461 tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt agttggtctt
     2521 gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct tcaaacaagt
     2581 gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt aaaaaaaaca
     2641 acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata aaaaaggtta
     2701 taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga acaaaaggtg
     2761 ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt gctttattca
     2821 aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt ccgcggatat
     2881 attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga ataaaatttg
     2941 tattctctgt cattttct
//
LOCUS       DACDMM010000221         2831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDMM010000221 DACDMM010000000
VERSION     DACDMM010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.226"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            803..1069
                     /locus_tag="IWD16_002648"
     CDS             803..1069
                     /locus_tag="IWD16_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089624.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1127..1639
                     /locus_tag="IWD16_002649"
     CDS             1127..1639
                     /locus_tag="IWD16_002649"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9089625.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1636..2673
                     /locus_tag="IWD16_002650"
     CDS             1636..2673
                     /locus_tag="IWD16_002650"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9089626.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1023 a          496 c          564 g          748 t
ORIGIN      
        1 ttttattgat gaccacgaaa gtgcatggcg atatttgatg ttgatacttt atgcagtacc
       61 ctatgtattc ttcgctatca ttctgtggct catagcattg ttttttagag tatttaatga
      121 cctttaggta gatttatata taaaaagggc taaatacgca aaataccccc ttaaaatgca
      181 aaataaatgc aaataagggg gtaactcctt atgaaaaaac aactaatagt attataacac
      241 gtttttatgc cgagaaaact tattggttgg aatgggctat gtgttagcta acttgttagc
      301 gagttggttg gacttgaatt gggattaatc ccaagaaagt accaactcaa caacacataa
      361 agccctgtag gttccgacca ataaggaaat tggaataaag caacgaaagg agttgagtaa
      421 aatgagtgag aaaacgaaga aaatcattcg agtatcatta gctttttcac aagtagtagt
      481 gcaatcattg atagtttatt atacttacca gcaatataaa ttatctaaag agaattaagt
      541 tttgattatg taataagttt tggtactaaa aaagacttga tctaattaga ccaagtcttt
      601 tgttagtgtt atattaataa caaaataaaa ataagaagtc gctcactccc cgaccaaagt
      661 ttgtgagcaa aataaacagt cgatgtttgt ttcttaattg tatatcttga tattaaacga
      721 tttaagatta ttcttcaaga tatatattcg caaatgtaat tggaaaagcg acttcttaaa
      781 caaaattaag gagtcgcttt ttatggcaaa aataacgaaa aatgagcaag aaaatttggt
      841 ggaaaattac acaaaaaaac cggcgtactc taatagccgg ttagacgcac atttcgtgtg
      901 cacttctaat ccgaaattga gctttgacgc tatgacgata gtcggaaacc ttaataaaaa
      961 cagcgctaaa aagctatccg attttatgag tttagatcca caaattagac tttgggatat
     1021 acttcaaaca aaatttaaag ctaaagcact acaagacccc ctatgttagg ctagttgtcg
     1081 tataggataa taagttataa taaagactat acggaggatg attaccatga caaataaaaa
     1141 caatgaacgt tactctaccg aactgaaagc gacagccatc aagaaaatga tgccacccga
     1201 aaataggggg attcgccaat taagtcaaac tttgggcatt ccagaagcga ctctttattc
     1261 ttggcgtaaa aaagctagaa ttcaagggaa tactacaccc ggaacgaaaa aaaatgtcga
     1321 aaaatggtca agtgaagata aatttttaat tgtgatggaa tcattctcaa tgaaccaagt
     1381 agaacttgcg gaatattgcc gaaagaaggg cttatttaaa gaacaaattg aggcttggaa
     1441 gaaaacgtgc ttatctgcga atgaacagga agaaaatcga acgagagaac ttgcaactga
     1501 actaaaagaa gagaaaaaac aagcgcgtca attagagaaa gaattacgtc ataaggaaaa
     1561 agcattagcg gaagccgctg ctctcttatt actaagaaaa aaagcacacg cgatttgggg
     1621 ggaccaagag gaagaatgat ttcatcttca gatcgcaaac aagctgttga acttattcaa
     1681 gaagccaatc gcaatggggc ccgtctcacc tatgcctgta atgagctgaa tattagcgtt
     1741 cgaacctacg aacgatggac gcgtgaaggt acgatagcgc atgatcaacg ccctttggcg
     1801 aaacgtcctg tacccaaaaa taaactgacg gatcaagaac gcgaaaaaat aatagaaacc
     1861 gtttcaaaaa aagaatttat gaatttgcca cctagccaaa ttgtaccaaa acttgcggat
     1921 tgctcgattt atattgcatc agagtctagc ttctatcgtg tattgcgaga aaaaaatatg
     1981 caacatcacc gtggccgaag tcaagtttca caaaaacgca taccgccaag tcaccttgcg
     2041 acgaagccaa atgaagtttg gacttgggat attacttggc taaaaggtcc aataaaagga
     2101 ctcttttacc ggttatatct catcattgat ttatttagcc gtaaaatggt aggctgggag
     2161 atttgggaat cagaagaagc tcgtcatgcc gaaaaactta ttaaacagac ggtgcttaag
     2221 gaacgtatcc aaggacagcc gttggtttta cactccgata atggcagccc tatgaaagcc
     2281 gcgacttttc aagtcttact cgaaaaatta ggaattcaaa gttcttattc aagaccacga
     2341 gtgagtaatg acaatcctta ttcggaagcg gctttccgaa cgttaaaata tcgaccagaa
     2401 tatcccaata acggctttaa aacaatagat gatgctcgaa aatggacgaa agagtttgtc
     2461 gattggtata acgattctca tttgcatagt gggattcaat ttgtgtctcc tgcagcgtgt
     2521 catgaaggac gacatctcgc tattttagag aaacgaaagc aggtttacga ggacgctaaa
     2581 aagcgccatc ctgaacgctg gtcaaaaggt gttcgaaatt ggactccaca tcaatcggtg
     2641 gcgttgaatc ctttaaaaga aacagaaagt taacaggagc ataatctttc aagattatca
     2701 aataaccctc gtcacccaat aggggaaaaa gttattcttc ccctattgga taacgaggcc
     2761 aaccgaccat agggaggttc tgctttttga taggtggaaa ataaaaatac gacaactata
     2821 ttgacaaaca c
//
LOCUS       DACDMM010000222         2804 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDMM010000222 DACDMM010000000
VERSION     DACDMM010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.227"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1306
                     /locus_tag="IWD16_002651"
     CDS             <1..1306
                     /locus_tag="IWD16_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9089627.1"
                     /translation="AIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVC
                     SLYLITMQDGNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQ
                     KIAKNVVRNLKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDE
                     VFINVDESVPYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINV
                     MTMRQAFIKRTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFK
                     MYKFRSMYMDAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIR
                     STSLDEFPQFYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRS
                     DITDFEEVVKLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            1338..2474
                     /locus_tag="IWD16_002652"
     CDS             1338..2474
                     /locus_tag="IWD16_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9089628.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            2474..>2804
                     /locus_tag="IWD16_002653"
     CDS             2474..>2804
                     /locus_tag="IWD16_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9089629.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPI"
BASE COUNT         1002 a          324 c          618 g          860 t
ORIGIN      
        1 ggcaattttt atagaacttg agaatcttac gattatattt ttatttgagg catataaaaa
       61 tgttttaaaa agaggatatt ataaagaggc tgtagcttct ataaagcaat cttttatgct
      121 gttacttgtg tgcagtttat accttattac aatgcaggat ggaaatgatt attccagagt
      181 ttctttattt ataatggggg tggtgtatgg attattaaca tatataattc gtatactttg
      241 gaaagggttg ttgcatcacc gcatgaaaaa cggtgaagat gtatcgttag ttattgtaac
      301 atctcaaaag attgcaaaaa atgtagttcg taatcttaaa gagaagaatt atggcatgta
      361 taaacttagt ggtgtgattg tagtagacag agatatgtat ggaactacca tcggaggtgt
      421 cccagtagtt gcaaatgctg ataatgcagc ggaatatctt ttacagaatt gggtagatga
      481 agtattcatc aatgttgacg aaagtgttcc atatcccaaa gaattaatcg ccagatgttc
      541 ggaaatggga cttgctgtac atatgaatct tacaaaagtg tctgatagta cacgtggaaa
      601 acaatttatt ggccgagttg gtgattatac tgttattaca tctagtatta atgttatgac
      661 gatgaggcaa gcatttataa agagaacgat tgacattctt gctggaatta ttggatgtat
      721 tgcaacaggt gttattttta tcttcgtagc accagttatt tacatcaaat caccagggcc
      781 gattttcttt gcacaagaac gtattggtca aaatggtaag acatttaaga tgtataagtt
      841 ccgtagtatg tatatggatg cagaagaacg caaagcagag ttgatgaaag aaaataagat
      901 gagtaataat ctcatgttta agatggattt tgacccaaga atcattggca ataaaatact
      961 tcctgatgga acaaagaaga caggaattgg acaatttatt cgatctacat cattggatga
     1021 gtttccacaa ttttataatg tcctaatggg aaaaatgagc ctggtggggt ttagaccttg
     1081 cttgaagtca gaatatgaga agtatgattt tcatcatcgt gcgagaattg cgatgaaacc
     1141 tgggattact ggaatgtggc aggtgagtgg tagaagtgat atcacagact tcgaagaggt
     1201 tgtaaaactt gatgcagaat atatcaggaa ttggagtatg ggattagatt ttagaatttt
     1261 gtttaggaca gtcaaaactg tgcttcatag aaacggttcc atgtaagata aagatactat
     1321 agaaggagta aaacgtgatg gcaagaaaaa aattaaaaat ctgtatgcta ggtcataaaa
     1381 gaattccatc aagagaaggt ggaattgaga tagtggtaga agaattggca acgcgaatgg
     1441 ttgcacttgg gcatgaagtg acttgctata atcgaagtgg tcatcatgtt agcggaaagg
     1501 aatttgacca gcataaaaat aaagaatata aaggtgtaaa attaaagact atttttacat
     1561 taaatataaa aggaattgca gcaatgagtt cttcagtatt tggtggaatt agagcagcat
     1621 tcggaaaata tgatgttgtt cattttcatg cagaaggacc atgtgctatg ttgtggttgc
     1681 caaaactttt tgggaaaaga tgtgttgcaa caattcatgg tctagatcat caaagagaaa
     1741 aatggaacaa gcttgctagt acatacatca tgttagggga aaagtgtgct gtaaaatttg
     1801 cagatgaaat aattgtactt agtgaaagcg tccaaaatta ttttgaagat atatatggaa
     1861 gaaaaacaag gtttatacca aatggcgtta aaaaaattga aataaaatca gcaggattga
     1921 ttacagaaaa gtatggatta acaaaagatt cttatatttt gtttttgggg cgattggttc
     1981 ccgaaaaagg aattcggtat ctaattgagg catttaaaga tgttcagaca gataaaaaac
     2041 ttataatagc cggaggaagc tctgatacag atgaatttgc aaatgaacta aaggaattag
     2101 caaaaggtga tgagcgaatt atatttactg gatttgtaca aggacaggaa ttagaagaat
     2161 tatatagtaa tgcgtatatc tatacacttc caagtgattt agaaggaatg ccattaagtt
     2221 tgttagaagc aatgagttat gggaactgtt gtatagtttc aaatatctca gagtgtacgg
     2281 aagtagtaga agacaaggct atgattttta agaaatcgga tgtaagtgat ttaaagataa
     2341 gattagaaga ggcatgcaat caatcggaaa tggttaaagt tttgaagaat caagctactg
     2401 agtttatttg tagtaaatat aattgggata aaatcgtaca agaaacatta aatttgtata
     2461 ggggaaatat ataatgattc gaatattaca gtgtgtgaat gatatgcatc gtgcagggtt
     2521 ggagacgatg ctaatgaact attataggaa tatagatcga actaaaattc agtttgattt
     2581 tttgacacat cgtccatata aaagtgatta tgatgaggaa ataataagtc ttggaggaaa
     2641 ggtctattat gcaccgagac tttatccaca aaattatcct aaatatttta aatggatgga
     2701 acaatttttt aaggatcatc cagaatatga gattgttcat tctcatattg atgcgatgag
     2761 ttatttacct ttaaaagctg caaaaaaagc tgggattccg atac
//
LOCUS       DACDMM010000223         2666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDMM010000223 DACDMM010000000
VERSION     DACDMM010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.228"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1113)
                     /locus_tag="IWD16_002654"
     CDS             complement(<1..1113)
                     /locus_tag="IWD16_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9089630.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDK"
     gene            complement(1243..2619)
                     /locus_tag="IWD16_002655"
     CDS             complement(1243..2619)
                     /locus_tag="IWD16_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9089631.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
BASE COUNT          813 a          510 c          381 g          962 t
ORIGIN      
        1 tttgtctaca gtctgcgcat tctcattaga gaacactccc ttttttgtga cttttctgaa
       61 gctagagtga atactttcaa ctgcattagt agtgtacatg atttttctaa tggcgctgcc
      121 ataatcaaag agttgttcaa catgagaaaa gtttcgtttg catacatcta ctgcaccaga
      181 ataatgagac caccgatttt gaaaactgtc gaaggcagcg tgtgcagcgt ttaaagaaga
      241 agcactgtag aattttttca tgtctcgaca gacttcctta taatccttac ttgggaggta
      301 tcgtaacgca tttcgaacaa gatgaacaat acagcgctgc acaatgacag aaggaaaaat
      361 tgcttttgcg ccttctttga gaccggaaac accgtccatc gaaatgaaaa aaacatcttc
      421 tacaccacct actttcagtt catcaaatac ttgcatccag cgatttttag attctgtttg
      481 attgagccat aggcctaaaa tctccttgtt tcctttaaga tcatagccaa gaatggtgta
      541 cacagcatat tctttagctt catagttttc gcgtaaagtg acgtacatac aatcaacgaa
      601 tagaaatgca taacacttgg ctaatggccg agcttgccat tcttccaatt caggaaggac
      661 ggcatccgtg atatcagaaa tcatttcgtg ggaaatatca aagccgtaaa tatcttcaac
      721 ggttgcggcg atatcgcatt gactcattcc tcgtgcatac atggaaagaa ccttaccttc
      781 gatgtcggag acatctcgtt ttctattagg aatcaactca ggttcaaaag aagcctcccg
      841 gtctctggga acatcaatgt ttactttacc aaagctcgtt ttaagcgtct tattgccata
      901 accgtttcgt cgattgtcat gtttctttgg ctctttagaa tgggcgtcat accctaaatg
      961 gttattcaat tctccttgaa gcattttttc aaataaggga ccaaacacat cttttaaaac
     1021 atcttgcata tcctctacag attcgggttg ataagtattc aaaatggatt cagctaactt
     1081 ctcagcatca ggatttcttt tctttctagc catcgtgtaa tcacctcttg atcttatttt
     1141 agaaaaataa aaaccgtgaa gcaaccaccc gcctaggatt gatggttact tacacggttt
     1201 atattacact ctcatttttg gtgctaaaaa gcaccgaaaa ttttaattat tgtcaattaa
     1261 taattctttt ggattccttc ttaatatatt gaatgaagaa ataatcaatg atacaagaag
     1321 tactaaactc ataaatataa ctacataaat catgaattta ggtaatacat tgatatctaa
     1381 acttgataat gtcttgttaa atccttcagc ttctgcccca ccacctaatt ggctagatgc
     1441 agattgttta gctatttgtt tagctatatc accagtaacc ttattcaata tattattccc
     1501 aagcttgtca gctgtatatt gagctaagaa ataggaacca acgaatgcgg ggattgatat
     1561 aaataccatc tcaattagga attgaccaaa aatttctaat tttgatatac ctaatgatag
     1621 taatactgct atttctttct ttctagcatt catccataag aataataata atgaaactac
     1681 aactcctgca aatattaatg agccaaaaaa taatttattt gaaattgagt aaatacctga
     1741 tatagattgt tgtaatgcag ggtaatttga tgaactttta atcaaattat attctctcca
     1801 atttatatct aatttcccaa gatcttttat tacactatca agattcttat caccttttac
     1861 aaagaatgtt gcatcttggt atacagctgt atcttcggta ttaccataaa ctttagcagc
     1921 tgtatgaata tcagtgatta atgtattttc gtatagttct tgtgctgcac ttactccacc
     1981 gctattatgc ccatcgaata gacctttaat ttctacttct actgtttcat ctgcaccttt
     2041 ctcattatca gcatcaaata aattagattt tatttttatc ttatctccaa ctttaaggtt
     2101 atttttttca gctaaatctt tatgcattaa gattttattt ttatcttcat tttctaaatg
     2161 ctttccttct actaatttat atgcttctga tacaaatttt gtttcttttg ttgagtcgtt
     2221 aaccccagtt aacataactg ttcttttaaa attctttgct ctttcaggtg attgattcgc
     2281 aagagtctct tgagtttcaa taatatcata atcaactaaa tctgcaacac tgtttattct
     2341 ttttacatag gagtctatac tatctgtttg agatatcttt ttaatatctt caccttttac
     2401 atttccccca cctctaggtg ttcctggatt tacttgtcta tttatctcca tagaaaaact
     2461 atttgttata ttagcaaatg tttcttttga agctctgtcc gtagcatctt taatagataa
     2521 actaatgaga cttaaagttg acatagataa aataactaat atgataatta atgattttaa
     2581 actttttcta gttacataag caaatgcatt ttttatcatt attcaacctc caaattcatt
     2641 ttatttactt ttttcaactt tttacc
//
LOCUS       DACDMM010000224         2590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDMM010000224 DACDMM010000000
VERSION     DACDMM010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.229"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1350
                     /locus_tag="IWD16_002656"
     CDS             <1..1350
                     /locus_tag="IWD16_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9089632.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..>2590
                     /locus_tag="IWD16_002657"
     CDS             1484..>2590
                     /locus_tag="IWD16_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9089633.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLR"
BASE COUNT         1013 a          360 c          514 g          703 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga
//
LOCUS       DACDMM010000225         2552 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDMM010000225 DACDMM010000000
VERSION     DACDMM010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.231"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            174..410
                     /locus_tag="IWD16_002658"
     CDS             174..410
                     /locus_tag="IWD16_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9089634.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            429..644
                     /locus_tag="IWD16_002659"
     CDS             429..644
                     /locus_tag="IWD16_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9089635.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1347..2099)
                     /locus_tag="IWD16_002660"
     CDS             complement(1347..2099)
                     /locus_tag="IWD16_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9089636.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2114..>2552)
                     /locus_tag="IWD16_002661"
     CDS             complement(2114..>2552)
                     /locus_tag="IWD16_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9089637.1"
                     /translation="LKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFD
                     CIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGI
                     TTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT          831 a          406 c          416 g          899 t
ORIGIN      
        1 tccacaaaaa ctttgaaaac agttcttgaa cactaacttt ttcattcctg aatcaaattt
       61 attttggaaa ttttactatt caagaataaa cttgataagt ttcaggaaga tttttactgt
      121 taatcaaata cgtatgttac agtagcaccg taaaaaatat tatttaaaga agcgtgttta
      181 aacatgcaaa atgtaaagga actaagtatt gtggaaatgc acaaactgtt ggtggaggca
      241 ataatgcttg gggaaaattg gggcaagttg taggtggact taccacaggg gcagtaggtg
      301 gtgcaggttt aggaacagca atttgcggac cagcttgcgg tgttgttgga gggttatatg
      361 gagctgtcgc tggaggtgct gcagctggct gggatgctcg aaaaaaataa aaaaggagaa
      421 taagtagtat gcatattaaa aacacaaaaa caacatttat attatcttca gaagagttga
      481 aaaatattca aggtgggagt gccgtgggag ttttaggcac tactttttct ggggcaacag
      541 caggagtaaa actttgttca gccggaggac cttatgcaat agctgcttgt ggtgtaggtg
      601 gagctttatt aggtgctgga ttcagcatgt ggaccggtgc gtaaacaatt aaaaaatatc
      661 aaacctcaag attggtttgt gtcgattacg aacataggta tgttctttat cttttcaaaa
      721 gtttggaatg gacagaactt tttactaaag cttcttgtgc tagtttttat aattatatta
      781 aatttttatc ttttattaaa gtcagataaa aattctttgg aaatgtaatg gtagagatat
      841 ttttacaaaa ggacttgcag cgctgtagtt ggtgctgcag gaggacctat ttcggccgtt
      901 acaggaggta ttggtaatgt acttgtcgga tgtgcattaa agcctgttcc tgcaagttaa
      961 atttataaaa acaggaaagt aattatctca aatcctatca agtacaatca taggattgca
     1021 cttgacagga caaatttttt aactaaaaaa tgaaaaacac tttctaaaaa ttagtagaat
     1081 ttgccaaatc tttcgcatct tccaccatac atttaatttt aattcccctt tatggtactt
     1141 aataaaaaca agaaaagaac tattcatttg gccctttttt ggttccagac catagaaata
     1201 ttcctccagt aactaatatt gctgttatgc acgtaactaa taacagctat aaaaattggt
     1261 acaactttaa taggattcag aaaaatttgc actagtgaag gaatctattt tattagaaaa
     1321 atgcaatctc cctaaagaaa aaaccactaa acagtgcttt ctttcaactt ctccttaatt
     1381 ttttgtgctt tccctaaagt aaagtttctg gccaactctg aatcaaaata tagtgaacgt
     1441 tttttaaaat ctatttcttt aacgttaaag atatttacta aacacgctcg actaattctt
     1501 gttaacatag gatatttctt ttcaagctca ctaattcttc cataaaattc gtactgccca
     1561 tctttagtac acaatgataa tctatgtggt aattttgaag cttctacaaa ataaatgtca
     1621 tttatatcaa aagtaaatgt ttgagaacct atggaaaaaa caaatgcttg attcgtaacc
     1681 tgttttgttg catctatcct ttcttgcgct aataacaata actcaacaat ctcgtttcga
     1741 tactcatcta gtgtttgatc ttttgttaca aatcctaacg tttcaactct tctttttatt
     1801 gttacgggta acatctcatc atgagtagtt gtaaaaatga tttttgcttg aacatcatat
     1861 tttctgatta cttctgctaa ttctataccg ttaacttcat gatttaaatc aatatctaag
     1921 aaatatattc cttgctttgg tcggaattgt ttgaggtatt ttttaacttc taacggactt
     1981 tgtgttttca atactatctt aaatacttta tcgtgaaaaa gaatgaaatt atcaataatt
     2041 cgttctaact gatttaattg tataatttga tcttcacata atattattgg ataactcata
     2101 aatcctcctt ccctcaaaac aacaaaataa cttgagtagt aaattgatac ggatcgtttt
     2161 gatagttcac aaacatattg ggattctttt tattgatttc ctctatacta gataaaccta
     2221 acccttgatg tccctttttt gttgttattc cttttttcat tagagtatct atagagagta
     2281 gtatctctct acaactattc gatatcgaaa tttctatctg tttatcatct cggtaaatag
     2341 ctagagacag ttctctatct tgactctcta tagatgcttc aattgcatta tcgagaataa
     2401 ttccaagcac cctaatacag tcaaatattg gaacaggaac ttcaataata gtacttggac
     2461 attcgaattt acaacaaatc ttattgttat ttgcttggta tagcttcgct atcaacaaac
     2521 ttttcaaata ttcatttttt atatttttta aa
//
LOCUS       DACDMM010000226         2525 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDMM010000226 DACDMM010000000
VERSION     DACDMM010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.232"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..1452
                     /locus_tag="IWD16_002662"
     CDS             1..1452
                     /locus_tag="IWD16_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9089638.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="IWD16_002663"
     CDS             1801..2433
                     /locus_tag="IWD16_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP9089639.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT          941 a          369 c          500 g          715 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaa
//
LOCUS       DACDMM010000227         2500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDMM010000227 DACDMM010000000
VERSION     DACDMM010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.233"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            181..1116
                     /locus_tag="IWD16_002664"
     CDS             181..1116
                     /locus_tag="IWD16_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP9089640.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            1206..2309
                     /locus_tag="IWD16_002665"
     CDS             1206..2309
                     /locus_tag="IWD16_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9089641.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            2322..>2500
                     /locus_tag="IWD16_002666"
     CDS             2322..>2500
                     /locus_tag="IWD16_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9089642.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
BASE COUNT          912 a          445 c          519 g          624 t
ORIGIN      
        1 tggatttttt tcaaatttta aaataaagcg ttttttcacg aaatttgctg aaagaaaacg
       61 tttgaaatat tacatgggtt atttatcaaa aatagttgac ttataggcta aaaagattag
      121 aatgtataat gaatcacaaa gaaaaattgt ggactagaaa tggaatggtg gggtattaaa
      181 atgaagaaaa aacgttttgt aactggattt gccgtattag cattttcagc tctagtgtta
      241 ggcgcttgcg gcgcagatga caacggaagc tcaaactctt cttctgaatc atctacagca
      301 caatcttcga ctgcaaaaag cacaacagaa tcatctgcaa aagttgtagc tggcggagat
      361 ttgcaagacg gcacatataa attagaagaa aaaaattatt caaatggtta ccgtgcagtc
      421 tttgaaatgg ttgtaaaaga cggcaaaatc actgaatcaa aatatgataa cgtcaatgaa
      481 aacggcgaat caaaaacaaa agatgcagaa tacaacaaaa acatggaagc aaaatctggc
      541 acaaacccag aaaaatacat tcctgagttg aatgaccaat tcttaaaagc acaaagcgca
      601 agcggtgtgg aagtcgtaac tggagctact cactcatctg aaagcttcca aaactacgca
      661 caacaattga tccaagctgc tcaagctggt aacacagata caatcgaaat cgacaatggt
      721 gctgatttga aagacggcac ttacaagctt gaagaaaaga actactctaa cggctaccgt
      781 gtgcaattcg aaatgacagt tgctggcggc aaagtaactg aatcaaactt tgattatatc
      841 gacaaagacg gtaaatcaaa acaagatgat acagaatata atgaaaatat gaaagcaaaa
      901 agcggtacag aaccaaaaac ttacatccct actctgaacg acgaattcgt caaagcaatg
      961 ggcgaagaag atggctcacc tgctaatgta gaagttgtta caggtgctac acacagctca
     1021 cattcattca tcatgtacgc acaacaatta gtgaatgcag ctgaaaaagg cgacactcaa
     1081 acaatcgaag tagataatat cgtaacagaa aaataatcac ataatcatac taataaaagt
     1141 tggagggtgg gacgatagcc agattaggca gtcgtctcac ctttcaaaat gaagagagga
     1201 aaaagatgcg gaaaaaacta ctattttcct taatcacact gggcatctct tttatggtgc
     1261 taactggctg cagcaactca gcaaataaaa caaccactga aagttcagca gctaccaaga
     1321 tattgaaaga tccatattct gatgaacagt tcttgttagg gacttacgtt cgtatccgtg
     1381 tgtatgatga aggcaaagaa tcagcattga aacctgcctt cgatcgagta aaagaacttg
     1441 gtgataaaat cacgatcaat caaaaaggtt ctgagatcga tgaagtgaat gagcaagctg
     1501 ggatcaaacc agtaaaagtt tctgatgacg tctacacgtt agtcaagcgg gcctacgaat
     1561 atagccaaga ttctcaaggc ggattcgata tggcgattgg tgcaatcaca caattatggc
     1621 gaatcggttt tgatgacgcc agaaaacctt cccaagaaga aatcgatcag gcattgaaat
     1681 tagtggatta tcataagatc gaactaaatg acaaagaaaa aactgtttat ctaaaagaaa
     1741 aaggaatgat catcgattta ggtgcaatcg ccaaaggtta cattacggac gaagtagtga
     1801 aagtcttgaa aaaacaagga gtgaccactg ccatcgttga cttgggcggt aatgtctatg
     1861 tcttaggcca cagcccaaga ggcgaaaacc aagactggac agtcggcatc caagatccga
     1921 acatggctcg cggatctgtt ttaggttcta ttaaagaacg gaacaaaaca ttagttacct
     1981 ctggaattta cgagcgttat ttagaagtag atggcaaaaa ataccatcat ttgtttgatc
     2041 caaaaacggg ttatccgttc gacaatgata tcgcaagtgt cacgatcata acagataaat
     2101 caattgatgg tgacgggttg tctacagcag tcttttcaat gggtgtgaaa aaaggactgg
     2161 aatacgtcga aagcgaattg aataacggga cggaagcgat cttcgtgaca aaagacgata
     2221 aagtatatgt gaccgatcca atcaaagaca ccttcaaatt atcggaggac tcccattaca
     2281 cgatgggtga ccgttctgaa ctaaaataag gagtacgaac catgacacta aaaatctttt
     2341 tagaagtagt tgaaatacga acaaaggtgg ctagtgtatt tccttttgcg gtaggggtat
     2401 tattctctat cgcttttttt catgagatac attggctaaa cactttttta ttttttattg
     2461 gaatgctagt ttttgattta gcgactaccg ctatcaataa
//
LOCUS       DACDMM010000228         2461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDMM010000228 DACDMM010000000
VERSION     DACDMM010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.234"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(95..673)
                     /locus_tag="IWD16_002667"
     CDS             complement(95..673)
                     /locus_tag="IWD16_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9089643.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(693..1361)
                     /locus_tag="IWD16_002668"
     CDS             complement(693..1361)
                     /locus_tag="IWD16_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9089644.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(1376..2446)
                     /locus_tag="IWD16_002669"
     CDS             complement(1376..2446)
                     /locus_tag="IWD16_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9089645.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
BASE COUNT          704 a          545 c          380 g          832 t
ORIGIN      
        1 tatgaaaaac tacctatata aaaaaaacaa gatataacga tcttcgtcat atcttgcttc
       61 ttcacctaac tatttttctc cctatataac agctttacgc ttctcctaat acaccattca
      121 aatataaatc gatcaattgc gccacattta gcggttcttc cacaggtctt ttcgaccgag
      181 tgatgtaagg tgctaacact aagaaaaaat gccttgctgc gatttctttt gaaatcccat
      241 ttcttagcac agattggttt gctgaaaaga tttcttcaaa aggttcacga taatttttct
      301 tccatagaaa gaaaagatga tctcttgttt cctttgaaag attgctttca agatcgtgaa
      361 gcatcagaaa caaatcgacc agatgtgtct caatcaaaaa ctgcgccatt tttgtcaagg
      421 ttacttgaaa tcccgtttcg cttgtttgaa cgatttctct caaagctttt ccaacatctt
      481 ttaagtggtt ttctaaaact gccgtatata gtttttcttt atcaccgaaa taatgataga
      541 gattgggttg ggtgatccca actttttgtg cgatctctct tgttgaagtg ccgagatatc
      601 ccttggacat aaacaagtca gtcgcttctt tcaaaatttt ttgctgtgta tctttgttgt
      661 atcgatggac catttttcca cctgcttttt ggttattctt tttctttcaa tacaccatct
      721 tccatcataa atattctatc gcatttgtca atcagccgtg tatcgtgagt aaccatgatc
      781 gttgctttat ttttgtcttt tgattctttt gccaagatat cgactacttc tcgtgccttt
      841 tctgaatcca aactagcagt tggttcatct gctagaataa tggacggatc attgtacaat
      901 gcacgagcga ttgcgaccct ttgccgttct ccaccagata gttcttctgg atacttgttg
      961 atcaacttat ccacgccaag ctgctcaaat aattcttttg cttctttttg acgatgttca
     1021 tgtttgatct tatctactaa taataactgc ttttttactg tcagaaaagg aaccagattg
     1081 gaagcttgca aaataaagcc aatttcttta aaacgtagtt taacgcgttc actttctttt
     1141 tgattactga attcttgatc attgataaat acagagccac tgctaggcgt ctgcaatccg
     1201 cctgccaaag tcaaaaaagt acttttacct gaaccagaag gaccgatgat tgcaacaaat
     1261 tcccctttat cgatactcat ggttgtttct ttcaatgctt taactacttg gtcgccatct
     1321 tggaaacttt tttctacttc tttaaattct attgctttca tgctcttcct ccttcttatc
     1381 caattgcctt caacggatcg atttttacga ttgtccgaac agaaaatagt gcgcctaaca
     1441 cagctactac cagcatcaac aagctaatga cgccaaaaaa cagccaattg ctttggaatg
     1501 gaaccgcatc aggtagaaca agggatgttc ccactgttcc taatagacca agaagaatac
     1561 caacaaatga taaaatgaat gtttgtgcca ctacagaacg cgcaataaat cctcctgtga
     1621 tcccttgtgc tttcattaca ccaaagatat taatcttttg catcgtcagt acataaatga
     1681 aaatcccgat cacaatagcc gcaataacga tcaaaaatcc gatcataaat ccaaaagtca
     1741 atacttgcgc attatatcca ggtaattcat tgatgaattt agaaattttg atctgttcta
     1801 aatcttcagg taaattactg attttacctc tgacaactat tgcgttaatc cgtgcatttt
     1861 cacttgtatc ttctttttcg aagcggatct cttggtaagc attgatggtt gtatagagaa
     1921 ctggtgagac attgaatttg gcatgatccg taaagccagt tagtttaaac gtcttgtcac
     1981 taccagataa cttaactgta tctcctacgg ctagaccata ttcttctttc aaactaatat
     2041 cgcctactgc ctcatcatcg ttatcaaaag cttttccttc aacaagattg ggcatgatga
     2101 attgattttt atcgataccg aagaagctaa cattgatttt tccttcttct gtactgtctt
     2161 tactagtcac gactccaggt gtctgggcaa gatacgccac tttcccacct tcgacttctt
     2221 ctgctatctt cttagtaatc atggacatcc ctagatttga atttgcatct ttcgaaagca
     2281 caatcgaatc tgcctcccac ttgtcgactg ctgttcgatt atcttgagct aagccataag
     2341 ctaacccagt caaaaaaaat accaggtagg cgatcaaaaa catcacccct gcaactaacg
     2401 cataacgtaa ctttgaatgc ataatttcat ttaatgctaa aaacatttaa ctcctcctcc
     2461 t
//
LOCUS       DACDMM010000229         2425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDMM010000229 DACDMM010000000
VERSION     DACDMM010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.235"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..421)
                     /locus_tag="IWD16_002670"
     CDS             complement(<1..421)
                     /locus_tag="IWD16_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089646.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWP"
     gene            complement(1135..1425)
                     /locus_tag="IWD16_002671"
     CDS             complement(1135..1425)
                     /locus_tag="IWD16_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089647.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1633..2325)
                     /locus_tag="IWD16_002672"
     CDS             complement(1633..2325)
                     /locus_tag="IWD16_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9089648.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          700 a          459 c          354 g          912 t
ORIGIN      
        1 ctggccactg atagttcaag gtggttgctc tccaaccggt ttcccccatt actgtcaatg
       61 gtattaaaca gtatatagag caaaccaata ataaattttt aacatttatt ttctcaaaaa
      121 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
      181 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      241 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      301 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      361 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      421 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      481 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      541 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      601 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      661 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      721 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      781 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      841 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      901 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      961 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
     1021 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
     1081 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1141 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1201 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1261 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1321 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1381 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1441 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1501 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1561 actaaaaaaa agaggcagca gtatagatta ctgttgcttc tttactaatt taggtttact
     1621 tgttaagaaa gattatttac gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt
     1681 atcttccact tcatcttttg ctttttctaa cttttgaata tattgtttca agcttgagtt
     1741 ttcagcagtt gcttcgtcta atttcttttg tgtatctgtt aactgagttt ctacatttgt
     1801 tttttcttgt gtcagtgttg cgatttgttg attcaaacca tttacagtat tttgtaattc
     1861 tgaaagtttt gcttgtgctt cttgtagtct tgctgtccaa tctgcattca gatgattgat
     1921 ttcatcattt ttttggttga tctcatctag tttgacattg atttcatttt gtttgctttg
     1981 tagttgtgca tccttgctga ctaattgact ttctaagttt tgtttttctg ctgccgattg
     2041 atttttttgg ttttctaatt catttacttt tgtttggtat tcttggacat ctgcctttga
     2101 atcttgcaat gtctgttctt tttgtgctag tcgagcggta agatctgtaa gcgcttgctc
     2161 tttatctgtc aatgtatgat caagctgtgt cagattctca ttgattgtat caatatctgt
     2221 ctctccaccc cacaaaataa tttcatttgc gtaccgcgtg cctgtgtagc caattagaca
     2281 tagaaatgca acgacaatcc caccagtaat aaatttcttt ttcatttgca tagctcctta
     2341 atccaatact tttacatgct aaaagtaagt gatctcaaac acaatgtcaa gcgtttttaa
     2401 attacatgtg taattttaat ataaa
//
LOCUS       DACDMM010000230         2401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDMM010000230 DACDMM010000000
VERSION     DACDMM010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.236"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            389..1006
                     /locus_tag="IWD16_002673"
     CDS             389..1006
                     /locus_tag="IWD16_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9089649.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1047..1346
                     /locus_tag="IWD16_002674"
     CDS             1047..1346
                     /locus_tag="IWD16_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089650.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1380..1547
                     /locus_tag="IWD16_002675"
     CDS             1380..1547
                     /locus_tag="IWD16_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9089651.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1592..2197)
                     /locus_tag="IWD16_002676"
     CDS             complement(1592..2197)
                     /locus_tag="IWD16_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9089652.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          821 a          373 c          441 g          766 t
ORIGIN      
        1 aaaagtatca aaaagattta tttttaaccc taaattagaa atatatagtg ttgtaataga
       61 ataatagtgt taaaaaaatt gtaaaagtta aagaagtcaa attctgatgt gaaaaataaa
      121 atataccatg gaagacataa aagaaaaact ggataactag tttatttaat ggcagaccgt
      181 taaggcctgc catttttatg tattttagcg gaataaatat aatcatgcgg gagtgtaaac
      241 ttactgtttc aaaaagtata ctttttgaca tgccttgatt ttctttgttt atgtatggca
      301 taataaccac ataatacaat ttgaaacgtt tctgatttaa tctgattgtt ttgaaacaat
      361 tttagtgaaa aaagggtaga taaaaacgat gagaaaattt ttttatatcc gggtcagtag
      421 ttatgatcaa tcgatcgaac gacaactgat ttccgcgaag gaattaggaa tccctgaaga
      481 atatatattt gtagaaaaag ccagcggcaa agattttaaa cgccctgaat atcaattaat
      541 gaaacggatg tttcgaaaag gagatttact ctatctacaa tcattagata gactaggcag
      601 aaataaacaa atgattttag aagaatggcg agaattaact aaggaaaagc agattgatat
      661 ttctgtgtta gacatgcctc tgttagatac tgctagttac aagcaactag atgggttaga
      721 gacgcttatc tctgacttaa ttctgcaatt actcagctat atggccgaag acgagcggaa
      781 acgaattaat agccggcaaa gagaaggcgt agaagctgcg aaacaaaagg gtgtaaaatt
      841 cggcagaaag aaaattgctt tacctcctga atttcctgcg atctattctg actggaaagc
      901 aggaaagata actgcagtct atgctatgga tcaattgggc atgaaacgaa atacctttta
      961 tcgacgggtg aaagaatatg aacatacctt gcaccacttt tcttgataga tataacagct
     1021 attagacggg aagggagagt ttgaaaatgg acttaacgaa tcaaagggtc gtttttactg
     1081 gaacgctgca gcaaatgaca cgaacccaag ccatcggttt ggttcactct ttaaacggcc
     1141 attgtcaatc aagtgttact tcaaaaacaa atttcctggt ttgtggtcag tattctaagt
     1201 cgttgtttga tgattctctc tatacaaaaa aggaacaaac cgcgcgggac ttgattgcgt
     1261 taggtcatga gattaccatt ttatcagaag tcgaatttta tgcgttaatt agtcaacagt
     1321 tgaaagaatg gcaacggtat ttataatcgt tagtaaaatt tacaaggagg aaaattttta
     1381 tgaagcgaaa atcttatgat cgttggggca ttgaaataac tagttttaat cgaaatattg
     1441 cttttcacgt ggagtgtgac tgtggggaat tagcaaaaag tgtttatcaa aaaaattact
     1501 tttactgtcc taaatgccat cgccgatacg gggtacatgg aacatagaaa cgaaatagtg
     1561 cggtagaaaa gcgaatgaat gtagtatatt tctacaaatc attcgctttc tttattttat
     1621 aaatagttgg tcgagagatc ccgttttctt ttgcaatctc tgcaactgga atgccttgct
     1681 ttaacatgtc caccacggtt ttaaaaacta agcgtttttg cggatctgtt gagttaatag
     1741 aatactcgat tggtcgtccc ttatatcggc cggctttttg agccaataat actccttgcc
     1801 gctgacgttc acgaattttc tcacgctcgt tttgtgcaat ataacttagc agtgataaaa
     1861 acatatcgaa catagcggta tccaggagtt catttccagt attaaaatct aaaaactgtg
     1921 catctaatat ttttaacgct acttttttct gtttcataaa agcaactgta ttttttatat
     1981 cttcataatc tcttcctaaa cgatctagtg atgtaacgat gacgcaatca ccttcacgga
     2041 tataatttag taatttttga aactcaggac gctcagtatc tttaccactg gctttttctt
     2101 ggaataattt atcaatgtta agtccttcca tcattgccaa ttgtcgatcc aaattttgat
     2161 cttgtgaaga tactcgaata tagccaattt ttcccataaa aaactccttt ttaaaagtga
     2221 tataatttac atgtaaaatt tacttgtagt tttactgcta tcatagcatt tttaaaaata
     2281 aattttatgt agacttaaaa tttacttcat ggagggttta aatggatgaa atcaaaagta
     2341 acaaaaaagc atttggcatt tatttaggag aacctagtac agaacaatta aaccaatttt
     2401 t
//
LOCUS       DACDMM010000231         2400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDMM010000231 DACDMM010000000
VERSION     DACDMM010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.237"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IWD16_002677"
     CDS             207..2069
                     /locus_tag="IWD16_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9089653.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="IWD16_002678"
     CDS             2069..>2400
                     /locus_tag="IWD16_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9089654.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DACDMM010000232         2365 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDMM010000232 DACDMM010000000
VERSION     DACDMM010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.238"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            144..494
                     /locus_tag="IWD16_002679"
     CDS             144..494
                     /locus_tag="IWD16_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9089655.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            908..1726
                     /locus_tag="IWD16_002680"
     CDS             908..1726
                     /locus_tag="IWD16_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9089656.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1799..2158)
                     /locus_tag="IWD16_002681"
     CDS             complement(1799..2158)
                     /locus_tag="IWD16_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9089657.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          784 a          342 c          448 g          791 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag
      181 caatcattcc tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa
      241 ttgtcggttt tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt
      301 ttattggtaa gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa
      361 aaagaattga tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat
      421 ttgaaattga atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt
      481 tagtaaaaaa ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat
      541 ttttattttg atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca
      601 ttcagtaata aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac
      661 cagttataga aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag
      721 gctgacctgt gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt
      781 atgtatctag atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa
      841 taggctgctt taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag
      901 tgtttttatg aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac
      961 atttgctcgt actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat
     1021 gaaagctgtt gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc
     1081 gttatttcca aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa
     1141 agataaaaca gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct
     1201 tgatttatgt aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta
     1261 ctgtgaaaga ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag
     1321 attagaatgg gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac
     1381 tatgcctgtt gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct
     1441 attagtacct accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa
     1501 aactttcggc ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc
     1561 ttttggagat gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat
     1621 ggataatgca acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga
     1681 agacggtgtt ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc
     1741 atcaaaaaac ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt
     1801 acgaagcgtc tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat
     1861 tctttaaacg aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa
     1921 aggtagtatc gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat
     1981 atgctggaat ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc
     2041 cctctgctga agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta
     2101 aacgaggtac ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt
     2161 tcacgaccac catcgacttc cacagttgaa attgacattt tatttaacac acctattccc
     2221 ccttaactat aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc
     2281 aaatcgccaa tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg
     2341 taaaaattaa accgaagatc tgata
//
LOCUS       DACDMM010000233         2282 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDMM010000233 DACDMM010000000
VERSION     DACDMM010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.239"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            363..889
                     /locus_tag="IWD16_002682"
                     /pseudo
     CDS             363..889
                     /locus_tag="IWD16_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1044..1619
                     /locus_tag="IWD16_002683"
     CDS             1044..1619
                     /locus_tag="IWD16_002683"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9089658.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1662..1859)
                     /locus_tag="IWD16_002684"
     CDS             complement(1662..1859)
                     /locus_tag="IWD16_002684"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089659.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            1846..2028
                     /locus_tag="IWD16_002685"
                     /pseudo
     CDS             1846..2028
                     /locus_tag="IWD16_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          810 a          358 c          420 g          694 t
ORIGIN      
        1 tacaagacaa agtatagcaa gcaaaattct tctccacttc cagaattttt tattttcatt
       61 tttagaaaag tgaaaaaact gacaacaaaa gcttgtttct ttcactctat taatatcttt
      121 agtgaaaaag ctcgttttat atcgtattct tccagcgaat gtacttctca taagtccaaa
      181 aaaagctgcg aaaaagaaag tacctcggta cctacttttt cgtagctgca cataacttga
      241 gcaagtatta atcgaagata cttttgttga gagtgtaaaa tattttgtgt aaatagaaaa
      301 aaggaagtcc cttctgtaga atagagttac cacaacacat tcacagaaaa gaggacttcc
      361 atatgaacga ttttactaca gaaatcctaa agactctagc gaacaaaggc gatttgaatg
      421 gattattccg tgtccatttg gaaaaagctg tcaatacgct tctcaaaacg gagttaacgg
      481 ctttcctcga ttacgaaaag tacgatcgca ttggttttaa cacgggtaat tctcgtaacg
      541 gctcctatga ccgtacggtc aagaccgagt atggggaact tcatctccag attccgcgcg
      601 accgcaatgg cgagttcaag caacagactg ttcctgctta tagacggacg aatgacacat
      661 tagaggagac cgtcattcac ctcttccgaa aaggtattac catgtcggaa atcgcagact
      721 tgattgagaa aatgtatggg catcactaca cgccccaaac catgtccaac atgactaaag
      781 tgctgactga agaagtaaat gcatttaaag ttagaactct aaatgataag tatgtcgcta
      841 ttttcatgat tccttcagtt tttcagatga tataccactc aaaaaacaat atgagagatg
      901 ttcaggaata ccatctctaa tggcttataa attggtttta gggatagtag atgttataga
      961 catatcaagg aataaacgag cagctgcata aaagcagatg gaattaagga ataattgaat
     1021 tgtaaaagaa gggaataaat ttaatggcac aattatttta cagatatgga acaatgaata
     1081 gtggaaagag tattgagcta ctgaaagtgg ctcataatta tgaagaacaa gggaagagag
     1141 ttatcataat gacaagcgca atagatactc gatcaggatt tggaaaagtt gagagtcgta
     1201 taggtgtttc gaaaccagct attgcaatta cttctgaaat ggatgtgtac ggagaattag
     1261 tggagattat tgatcagtac ggaaaagttg attgtatttt ggtcgatgag tctcaatttt
     1321 taagtagaga gaatattatt gatctatcac ttatcgtaat gaatgaggaa gtaccagtaa
     1381 tttgctatgg attgaagaat gattttcaaa ataatctctt caagggatcc gaagcattat
     1441 tgacatttgc tgataaaatt gaggaaatta aaactatttg ccatttttgc aataaaaagg
     1501 ctgtaatgaa tgctcgtatt gttgatggta ttccagttta ttcaggtgaa cagattgtag
     1561 taggaggtaa tgaagattac ctttcggtat gtcgcaacca ctactacaac gaagattgaa
     1621 ataaaaaacg tgtaatcaaa taattgatta cacgtttttt attattctgt taaaggttga
     1681 ggatcagaaa tttttaggta tctataatcc tcacctaaaa atccgttaaa acgaagtaaa
     1741 acatcatgac catttaaatc agttggaact aaaattggaa ttaatttatc aacagtagtt
     1801 ttgttgtaaa gttcaaaagt gttctccatt ccttctggtt ctacaatcga atctttcaat
     1861 aaacaaatca agaaatacag ccgcagaaaa gagcagtttc aaaatgaaga atcactagag
     1921 cgtttcctag tatccatctt tgatacatac aatcaaaaat tcttaaatag aagccataaa
     1981 ggcttccaac aagtgacgga tacattagct tcaatgttta ctgagtaacc cattattttg
     2041 cagaaggacg agttatttac acaaaattat tgacgctccc tgtttggagt ggtaaaaaga
     2101 ttgagaaatt gtttcattag ctattacgat tattattgta atatattttt attgaaaatc
     2161 aaaattaaaa taattcactg taaaaacagg attatttttt ctattttggt aagtcaacag
     2221 gaacatagtt ttcaattgat tcaattgact cattttcaat aatctgatcc ataaatccag
     2281 gg
//
LOCUS       DACDMM010000234         2274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDMM010000234 DACDMM010000000
VERSION     DACDMM010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.240"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            180..554
                     /locus_tag="IWD16_002686"
     CDS             180..554
                     /locus_tag="IWD16_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9089660.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            559..987
                     /locus_tag="IWD16_002687"
     CDS             559..987
                     /locus_tag="IWD16_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9089661.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            1078..1992
                     /locus_tag="IWD16_002688"
     CDS             1078..1992
                     /locus_tag="IWD16_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP9089662.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            2108..>2274
                     /locus_tag="IWD16_002689"
     CDS             2108..>2274
                     /locus_tag="IWD16_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9089663.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKI"
BASE COUNT          856 a          360 c          395 g          663 t
ORIGIN      
        1 cacagaaatg cctagtaatt tacatttcag agtttgccga ccgtgaagtg tttattagtt
       61 atcaagtctt tttagctaat attttagctt acgaaaacag tatactaaca cttttattag
      121 ttgtcaacac ctttaactaa tttttttatt agtgttttcg tagcttgtga ggtgcgaaaa
      181 tgaatttact agatagaata aaggaattag ctcataaaag aggcataagt ataactcaat
      241 tggaagaaga attaaatata cctaaaaaca cgatttatca atggaaaaat agaactccta
      301 gtactaaaag actacaactt gtagcagatt attttaatgt aactactgat tatttactcg
      361 gtagaaacca agttcctgac tgggcaacaa aagatgaagt agttgaactt gataaattac
      421 tagattcaaa tgtcaatatg tcttatggcg gagaaacact gacgcctgaa caggtccaac
      481 gcgtaaaaga tatccttatt ggaaccttct gggatattgt gaaagaagac aaagaaaaag
      541 gcaaaaagat gtgagcttat ggagatggat acgattaatt tagtcgagga gttgaagcgg
      601 aaataccagt ccgctaatcc tttttatatt tgtgaaaaga tgggcattaa aattcaatac
      661 gttcctttta tcgagaatcc caaaggacag tttcaagaaa ttagaggtcg tgcaataatc
      721 ttcttaaatg atgaactgag agactctgag gaaagattct acatttgcgc tcacgaatta
      781 ggtcacgcta tttttcatcg tggcttatcc agttactatg tatcaacaag aacatctaga
      841 agcaaatctg aaagcgaagc taattgcttt gctgctaatc tcattgtttc tctatacaaa
      901 gaagacaatg atcaatatcc taaacgaatt gaggaattaa agaatcttta cggacttcca
      961 gaaagcgctt atcgctttct tatataatgt aaaaaaagtc cgtgctgcaa cacggactaa
     1021 aaaccttatt tctaagatcc tacaaaaaaa tcatatcata gaagtgagga attaaaaatg
     1081 aaaaaaattg tttttgggtt gcttttttta ggcttggtat taagcggatg ctccactagt
     1141 cctaattctg ctagcactaa agaaaatact gtttcatcaa catctgaata tgaaacaaaa
     1201 aaactaactt catcagacgt tcataaaata aaagttggtg atgatttaac tcaagtttat
     1261 cagacacttg gttcaccgtt acgtaaatgg acaaatgatt ttgtttacaa agaattagac
     1321 aatgctatca cacgggatga attaattgta tctatttcaa aggatgcaca aggagattat
     1381 tataaagagc actctgaatt aattgaacat ggaaatagtg caaaagatat aaaaaaatta
     1441 gagatgctcc aatatactta ttcaactgat tctgatgatg aaacaatgtt aatttgggtt
     1501 agtccaaaaa ctgaacaggt tgtatattat aatcaacgaa ctttcttaga taaagatgga
     1561 aaatccaaag attcaagctc tactacaact acaactacaa cttcgtcttc agaaccccag
     1621 acggcatcag ttgcacatag tataggagaa gcagttcctt ttacttctga aaatggtaac
     1681 tctattaacg taactgtcca gtctgtatca aaagattacg gtgatgattt ctataaacca
     1741 gaaggcctat tttacgcaaa ggtagatttt cttgtagaaa acacagggac tacacctttt
     1801 gatgcaagtt caagttattt agaattttat gattcatcag atattaaatc tgatttagct
     1861 agtagagatt acttttcgga aaccatccaa ccgggtaaat ccgcacaagg aacagcatat
     1921 tttgaagtga aaaatgatgg aacaacattt gaagtgttct tcgcagatac ttcatggaaa
     1981 ggtagctact aaacaaaaaa acacgccccc tccctcgcca aagtttgtgg acgtgatgaa
     2041 aaataaacct gctataattg gcttacttat ccattcctat tatagcaaca aataggagat
     2101 gaaaacaatg tggattgaaa ccaaaactga taaaaacgga aaaaaagtat ataaatataa
     2161 tgagcgatat attgatccaa aaactagaaa aagaaaaaaa gtgagcatta cttataaaaa
     2221 taaatctcga gaaactcaga aagtggcatt gctagagtta aataaaaaaa ttga
//
LOCUS       DACDMM010000235         2262 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDMM010000235 DACDMM010000000
VERSION     DACDMM010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.241"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            279..821
                     /locus_tag="IWD16_002690"
     CDS             279..821
                     /locus_tag="IWD16_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089664.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            977..1426
                     /locus_tag="IWD16_002691"
     CDS             977..1426
                     /locus_tag="IWD16_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP9089665.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1456..>2262)
                     /locus_tag="IWD16_002692"
     CDS             complement(1456..>2262)
                     /locus_tag="IWD16_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP9089666.1"
                     /translation="TYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGL
                     QLSRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDL
                     TYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDR
                     GKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLR
                     LELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          785 a          372 c          429 g          676 t
ORIGIN      
        1 acatgaaaaa tcctccagtg tgttttttat tattctacac accttatttt ttctgtctag
       61 tctagtgtag ccgattcaaa ttgatgaagc agatcccaaa gggctgcttc atcaattttt
      121 tcgtaagtgt ccgtacactt actgtgcttg ctcatatatt aatcgatcat atactaatcg
      181 tgtaagaaca actttaagca taatacttat attgttatac catagtttgg aaaaataaaa
      241 aattatattt catcaacgtt tcataaaagg tgtgtagaat gttattgaaa gtagctataa
      301 aatgctattg tgtaaaaaat aaaggaggaa taatttatga aaaaatagta ccaagaggag
      361 catgcctatt acttgcatta tgtttgagct taagcgcagt tgggtgtcaa aaaagtaatg
      421 aaaacaataa aataaaagaa ggacaatcca taagttctga agaggaaggc atgtcaatta
      481 atgcaaatgg caaaaatgaa acttttaagc catcagatta tacattggag gcaaagaaag
      541 aatatgttta tgaatattta gggcttaaat ttaaactctc agacaaattt agaaattata
      601 tagctgataa aaagatagcg atgttagatg atcaaagtcc tatagataaa gaactaaagt
      661 atgctatttt gacatttgag aaaatgacag aagaacaaaa gaatgcagtt atagaaaaga
      721 tgggcgatgg atataaaaat tggcaaaatg agcttgaaag aattgggact attggtatat
      781 ttgaaaaaaa tacatcagaa gaaaaaaatc taaaattata aaatgtgata ctcatactaa
      841 aataggagtt tcaagtgatg gaaaatacga ttgctattta agcacaaata gcggagctga
      901 aagtaatctt ctggatgaat taaaaagaac tgagattcaa attatagata aaaaagaacg
      961 tcctaaaaat ggttttgtgc tatcagaaaa aactgattta gaaaatacag aagcatttaa
     1021 caaagaatcg gttaaagatt tacgtaaact accaacaaag gacataaatg gcaaggattt
     1081 tacaagtaaa gattttgaga agtatgatct tactatggta aatgtatttg caacttggtg
     1141 tacagcttgt gtaaaggaaa ttcctgattt ggttgaggtt cagaatgaaa tgaaaagcaa
     1201 gggtgtaaat atcgttggag ttgttacaga tcctgttgat gataacggtg aaaacaagga
     1261 agcaattgaa aagtcaaagt taatacatga aaagacaaag gcttcatatc ctttcttaat
     1321 gccagatgga atggcaacac tttttttgac aaaaattata aggtgttgtg aatgctgcgg
     1381 tgttatggct acgattgccc ttgacaatga gcatcctcgg atctgatttt ctccggcaga
     1441 agcacaatca ttcaattacg ctgcctctcc agaggtatca gatccgcgct cattatcaag
     1501 gattcgcttc gccaatctct gttgtcgaat ttggatcggt gtttcatacg ccaacgtgcc
     1561 atgtaagcgt agatagttcc accaatgcac ataatcaaac aattctagac gtagctgtgc
     1621 cagtgtctca aattggtatt gatgcacgaa ttctactttg acagatttat aggttgattc
     1681 cacaacggca ttatcatacg gacaaccctt cctactcaat gaacgagtaa ttccaaaacc
     1741 attcagaatc tcatgaatgg tttggttatc aaattccttt ccccggtctg tatgaaaaag
     1801 cttgacgtct gttaaagaat acggtatccg tccaaaggct tcttttacca atgaggcatc
     1861 tttcttctca ccacaggaat aaccaataat ttctcgatta aacaaatcaa gtattaagca
     1921 gatataatgc cactttttcc ccacgcgaac ataagtaaga tccgtaacga tggcttccaa
     1981 tggctgttct tgtgtaaagg tccgatctaa tacattcgct gttttcgctt cattacaagc
     2041 tgttcgttgc cctttaaaat gagcgatcgt ataggtagat gtcaatccgc ggcgtttcat
     2101 gattcgaccg attcggcgcc gactgagttg aagcccacgc tttgctaaac acttcttcaa
     2161 ttttcgggta ccgtaagcct ttcggtttcg aataaactct tcctgaacga tctcttctaa
     2221 gtcggactca ttttcgatcg gtttcgcttg ataatagtag gt
//
LOCUS       DACDMM010000236         2237 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDMM010000236 DACDMM010000000
VERSION     DACDMM010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.242"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            27..605
                     /locus_tag="IWD16_002693"
     CDS             27..605
                     /locus_tag="IWD16_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089667.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            1077..2117
                     /locus_tag="IWD16_002694"
     CDS             1077..2117
                     /locus_tag="IWD16_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9089668.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
BASE COUNT          753 a          248 c          457 g          779 t
ORIGIN      
        1 gaaactctaa ttttgaactt ttaagaatca tttctatatt gtgtattgtt gcttaccatg
       61 ctaatttgcg tttaaattat aatgcagacg gaattatagt acatggttca ttaactgcta
      121 accagatttt cttgaattat attggtattt ggggtcagct aggagttaat tgttttatat
      181 ttatttcggc atatttttta agtgagaaaa gaaacattca ctttgaaaat gttttgggaa
      241 ttgtattaga atgtattttt tatagtgtat tagtttatgg aattctttgt gcatgtggaa
      301 tagaaacatt ttcattgaac agctttgtaa aagaaatatt atcatttgct ctaggtagat
      361 attggtttat tacatcctat ttagcattgt atattctaag tccgtattta aatatcatgc
      421 ttaacaatct ggaaaaactt cagttgcaaa aaataagtat gttattgttt ataggtgtat
      481 gctgttatcg tatgttattt aaaacatctt tgtttgggga ttgtgctgtt ttaattgcac
      541 tatatgtcat atgttataca ctgaaaaaaa cgataataat atttttgaga gacacagaat
      601 cataggaaca attgtatgta taacagttat accacttctc atatccatag gaatactttt
      661 caaatttata ccaaatgcaa tggttaacca gatggttcat attggttctc cattgatggt
      721 tatagcaagt atatttttat tttatgtatt taaaaatttt tcattttaca gtaagtatat
      781 aaataaactt gcgagaacca gtttaggggt atatatttta catgaaaatc attatttcat
      841 tgatggtatg aagtctagag gcttaatatg ggatgggata tttggattag gcaaaaggtt
      901 tttagatagg gatttttgga tttggtttat aattgcagtt gtagttgtgt gcataatttg
      961 catggctatc gatgttgtaa gagaaaaatt tgaaagacag atttttaaaa ttaagagtgt
     1021 aaagaacctg tgtaaactta tgaatcatgt tataaatata tacccataat taataaatga
     1081 ggagaaaaga agtgaaagtt atatcattcg gagaattatt attgagactt gcagcacccg
     1141 gttattcaaa gttatttcaa agagatagtt tggagactac attctgcgga ggggaagcta
     1201 atgtagcggt atccttagca atatttggat atcaatcaga atttataaca aagcttccgg
     1261 attcagatgt tggatatgcg gcagctaatg ctttgcgtta ttttggagta aatgttgata
     1321 atattatatt tggagaggga cgcatgggac tgtattattt agaaaaagga gcagctcaga
     1381 gaccatctaa agtaatttat gacagaaaat tttctgcaat ttcgttagct gatagtgatg
     1441 aatttaagtg gaaggagatc ctaaacgatg caaattggtt tcattggaca gggataaatc
     1501 cagcattgtc tgagaatctc tttgagacgt ccttagatgc atgtcaggct gccaaaaaaa
     1561 gaggtataac aatttcttgt gatttaaatt atcgatccaa gctttggaca tcagaggaag
     1621 caaaagaaaa gatggaacag atactaccat tggttgatat ttgtatttgt aatgaagaag
     1681 atgcagaaaa agtgctagga attaaagctg agcatacaaa tattaatagt ggagaattaa
     1741 ataaagatgg ctatgtgcag gtggctaggg agattcacaa gaagtttgga tgtaggtatg
     1801 tagctacaac tctgcgtaga agttattcgg caagtagaaa tggttggagt gcaatgttat
     1861 acgaagcaga aaaagataaa gcgtattttt ccacagaata caacatacaa attgttgatc
     1921 gcgttggtgg tggagacagc ttttctgctg gtataatata tgcgttatgt gaaaatatgt
     1981 ccggacaaaa ggctattgaa tttgcgactg catctagttg tttaaaacat actatagaag
     2041 gtgattttaa tcgaactaca attgaggatg tatataattt aatgaatagc ggtggaaatg
     2101 gtcgcgtgtt gcgttagtca acatataaag ataaatcaca gtaattttta tggtgattat
     2161 ctgtcttagt agtgattaga ataacgtgaa aataaattgt tgagcgttta tactgttttg
     2221 aaaaataaat ttaacat
//
LOCUS       DACDMM010000237         2233 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDMM010000237 DACDMM010000000
VERSION     DACDMM010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.243"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IWD16_002695"
     CDS             <1..544
                     /locus_tag="IWD16_002695"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9089669.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="IWD16_002696"
     CDS             complement(900..1472)
                     /locus_tag="IWD16_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9089670.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="IWD16_002697"
     CDS             complement(1488..2093)
                     /locus_tag="IWD16_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP9089671.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt cta
//
LOCUS       DACDMM010000238         2227 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDMM010000238 DACDMM010000000
VERSION     DACDMM010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.244"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            95..>2227
                     /locus_tag="IWD16_002698"
     CDS             95..>2227
                     /locus_tag="IWD16_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9089672.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLP"
BASE COUNT          769 a          475 c          431 g          552 t
ORIGIN      
        1 aaaaagcttt tcgacacgat tatctgttcc aaaaaatgtc taatgaatgc tgaataagac
       61 agaaaatcaa tgaaaggaaa aggactagtt tattatgaaa aataaaaaaa cgatacgttt
      121 gctagctgct acttggctga ttgctccgtc tgttttgtcg accaataccg tatttgccgt
      181 agataatcaa atagctgatc aaacagtcac aacagatacg actacatcca gctcagctac
      241 tagtgaaaca acggattctt ttacagaaga cccaactgga acaactaact caacaacagg
      301 atcgaccgag ccaactgaaa ttgatccggc tgatccatca gaaaatgaaa agccagccga
      361 gacatattcc cttacaattg ctccttcttt gattggtaag atccaactga ctgcttcgac
      421 agcggattcc cctgaacagc aacctttgga tattacggaa agtggaacgg tagaaggttt
      481 attagcggaa acgaaggtaa cttacactat tacgccagct gaaggtgaac aaattgcttc
      541 agtcgctgtt aatggtgcca aagaagtaaa ttatgatgca aagaccttta cagtaggtac
      601 gaacaattta tctattactg cagaatttaa agctggaccg gatacttcaa cagagaaacc
      661 aactgatcct agtgaagaag tccctagtac accagatccg gaaacgccaa acaaaccaga
      721 tgtgggtaat aacaacaata ataatggttc aaataattca aacaataata acaataattc
      781 gaacagcggg aattcttcga atcaaacaaa cccatctgga tcgaataaca atggaaatac
      841 tcagagagaa ccaaaccatc caaacaatgg ttcatcgaat ggcggaacga acaatgcttc
      901 atctcgtcca aatacagcaa atcaaagcgg tatagagaat ccatcttctg cttcttctga
      961 ttttgttgta aaatcaccaa ttgatgctgt attgcctaca aatacaacga atgtccaaca
     1021 agcaatcgtt cgagaagcat tcaagcatct tggaaaacca tatgtatggg gagcaaaagg
     1081 tccaaatact tttgactgtt ctggtttgac ttattacgta tatatgaaag caaccggtca
     1141 ttatatcggt ggctggacag gcgaacagca atatgcaggt acgcagattc cagtaagtca
     1201 ggcccaacct ggcgatctcg tcttctgggg accttcttcc ggtgttactc accatgtagg
     1261 tatctatatt gggaacggac aatttatcca tgcgcctcaa ccaggagata aagttcgagt
     1321 aacttctatt tcagatttca cgcctgattt tgctgtccga gtaaacttag caggtttacc
     1381 tgctgcttca ggttcgctga caggcggctc atctatttta gatggattag atggctcgtt
     1441 ccatttcagt caaaaccaaa caactgatca gttcttgaaa aagattgcag atgatgcaca
     1501 ggaaatcggg caaaaagaag gaatctacgc atcagtcatg atggctcaag ctatccttga
     1561 aagtggatct ggaaacagct tattatcaag cgaaccaaat cataaccttt ttggtatcaa
     1621 aggatcatat aaaggaagca gcgtgacctt caatacactt gaacaagata gttccggaca
     1681 aagctaccaa atccgcgcac aattccgtaa atatccttct tacaaggaaa gtttggaaga
     1741 ctatgccgac ttgatcaaaa atggactgac tggcaatcct gatttctaca aaccaacttg
     1801 gaagtcggaa acaaaagact acaaagaagc aaccaaatat ttggaaggac gttatgctac
     1861 agatcgccaa tattcacaaa aattgaacgc gatcatcgaa gcgtacgacc tgacaaaata
     1921 tgatgaaccg aaaaaagaag cagcaacaac tgaagaagca aacgctgaaa tcctcaatat
     1981 gtctaagcga ttcgatgtgc caatcaaatg gacaacagat ggcgtgccaa tcacaggatt
     2041 agaattttca acgataaaac gcgtcaacaa cgcttcttcc atcttccgtg tcgcttcaat
     2101 ctgggatttg tggaacaatt ttacggcaag acagatacca gaaacaacta ctcgtacagt
     2161 taaagctgca actgttcctg tactaacctt gttaagttta gacaaatcat tctggcttga
     2221 tctgcct
//
LOCUS       DACDMM010000239         2220 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDMM010000239 DACDMM010000000
VERSION     DACDMM010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.245"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..345
                     /locus_tag="IWD16_002699"
     CDS             <1..345
                     /locus_tag="IWD16_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9089673.1"
                     /translation="EIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISS
                     TLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRA
                     RIYLQQGLIFEN"
     gene            400..1965
                     /gene="guaA"
                     /locus_tag="IWD16_002700"
     CDS             400..1965
                     /gene="guaA"
                     /locus_tag="IWD16_002700"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9089674.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT          688 a          451 c          455 g          626 t
ORIGIN      
        1 gaaatcgttg atgagctgtt aagctacaac gaagaattgc gaactgcgta tcatttttgt
       61 caattattac gctattattt cgttaaacga ggtacggaag gctttcaaga aacccttaaa
      121 acaatttcat ctacgttacc tcagtcgttt aaaaagaaat tcacgatctt ccatcgctac
      181 caatcgggta tttcaaatgc ctttaaggtg tcccactcaa acggcgtcac tgaggggctg
      241 aataacaaga tcaagctgat taaacgaatt gcgtttggtt atcgcaactt ttataacttt
      301 cgggcacgga tctatttaca acaaggtctg atctttgaaa actaaaaaaa tatgcggcgc
      361 acagaacgcg ccgcatatcg gactaagttt tattcaaatt tgtcttcacc aacacgattt
      421 gacgaagagc caaaaattat cgtacttgat tacggtagtc agtataacca actgatcact
      481 cgccgtattc gtgagttcgg tgttttctca gagttgatga gccaccgcat taccgcagaa
      541 gaagtaaagg caatgaaccc taaaggaatc atcctttctg gcggaccaaa cagtgtttat
      601 gatgagaagg cctttggtat tgatgaaaag atttttgatt tggggatccc agttttagga
      661 atctgttacg gtatgcagtt gattacttat aaattaggcg gaaaagttaa accagccaaa
      721 aaccgtgaat acggaaaagc agaattggaa atcacttctg cagaagcggc ccttttccaa
      781 aacactcctg aaaaacaaac cgtttggatg agtcatggtg acctagttac ccaagcaccg
      841 actgatttcg atgtcgttgc taccagcaaa gattgcccca tcgcatcgat ccaaaatgca
      901 gaacgcaaaa tgtacggaat ccaattccat gcagaagttc gccattccga atatggaaat
      961 gacttgttgc gccattttac actagatgtc tgccaatgta ctggtgactg gacaatggat
     1021 aacttcatcg acatggagat cgcaaaaatt cgtgaacaag tcggtgacaa aaaagtttta
     1081 ttaggccttt caggcggagt agattcctct gttgttggtg ttcttttaca aaaagcgatc
     1141 ggcgatcaat taacatgtat cttcgttgat cacgtattgt tgcgtaaagg tgaagcagaa
     1201 caagttatgg aaagtcttgg cggaaaattt ggcttgaaca ttataaaagt cgatgccaaa
     1261 gaacgtttct tatcaaaact tgctggcgtg tctgatccag aagaaaaacg taaaatcatt
     1321 ggaaatgaat ttgtatatgt cttcgatgat gaagcaacca aacttgccgg tgaagaaggc
     1381 gtttctttcc ttgcacaagg aaccctttat accgatgtga tcgaatcagg aactgaaact
     1441 gcccaaacca tcaaatcaca tcacaatgta ggtggattac ctgaagacat gcaattcaag
     1501 ctgatcgagc cattgaacac attgttcaaa gacgaagtac gtgaattagg tacccaatta
     1561 ggcatgcctg acagcatcgt ttggcgccaa ccattccctg gaccaggctt aggtattcgt
     1621 gtccttggtg aattgacaga agaaaaatta gaaatcgtcc gtgaatcaga cgctatcttg
     1681 cgcgaagaaa tcgccaatgc cggtttagat cgcgacatct ggcaatactt caccgtccta
     1741 ccaggcattc gctctgttgg ggttatggga gatggccgta cgtatgacta cactgtaggt
     1801 atccgtgctg tcacttctat tgacggcatg accgctgact ttgcccgtat cccttgggaa
     1861 gtcttgcaaa aaatcagtgt ccgtatcgta aatgaagtcg cacacgttaa ccgtatcgtg
     1921 tatgatatta ccagcaaacc acctgcaaca gtggagtggg aataaagagc caacataata
     1981 tggatgagga atacctataa atcaacatta ttcaattact aaagttcctg attttcgtaa
     2041 aaaataagat ggtttcctac cattttccct accaaagccc ttgttagatt aactactaac
     2101 aagggctttg cttttattca ctacctttat tttcctattg aattagcttt ttatctaatc
     2161 agatttttag tactataaat atatatatca gatttttcga tttaaaagat ctggtaaaaa
//
LOCUS       DACDMM010000240         2217 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDMM010000240 DACDMM010000000
VERSION     DACDMM010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.246"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            9..1379
                     /locus_tag="IWD16_002701"
     CDS             9..1379
                     /locus_tag="IWD16_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9089675.1"
                     /translation="MSFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIA
                     LTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLS
                     KQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISD
                     SIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWF
                     LFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSI
                     AAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVG
                     ESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNV
                     DVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEIN
                     KKNENK"
     gene            1366..>2217
                     /locus_tag="IWD16_002702"
     CDS             1366..>2217
                     /locus_tag="IWD16_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089676.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMG"
BASE COUNT          740 a          333 c          383 g          761 t
ORIGIN      
        1 cgattctgat tagtttttat tatggagaaa agttttttaa aaagatagat gctgcttttt
       61 tatttagttg tttatatact tttgcaactt atcgaacagt tgatatctat tatcgatccg
      121 caattgcaga agcaatcgct cttacgatta tcccagctgt tttatattat gcttatggat
      181 tgatattcaa tagagaaaaa aaagtcatcg ctttatctgt cagtataagt ctacttgtgt
      241 atactcatgt cttaactaca ctattgtgta gtgcattgat tgcttgtatg attatagttc
      301 gtttgttttt aagcaagcaa ataaaaaacg atataaaatt tgtggtcgtg aatttagtaa
      361 aggctgtttt tttgacttgt gcattaacta gctatttttg gtatcctatg ttgcagcaga
      421 tgaattatca agaaatacac agtcccttca aaacagattt gcagctagaa gcactttcta
      481 tttcagatag tatcattcaa gcgttaaata acgatattac aacatacact atggggttat
      541 taggattggt tgcgttgatt tttcctttat tcatactgaa agaaatggag aaaaaagaaa
      601 aaatcatcta tgttggcgta gtttctacat gggtatgcgt aactaattta tttccatggt
      661 ttttgtttca aaaaacaccg ataagcttac tccagtttcc ttggcgtatc ttaggtatcc
      721 aaatcttttt ttcttcaatg atcataacgc ttgtttttat gaaaaagact atgaataaaa
      781 aatattcatg gttaatcatt ggcgctactg tggtgtttct actcgtcacg tctatagcag
      841 caaaagaaaa ttacgcaaaa gttatccaat caaaacacgg tcatattgtt atcaatgaag
      901 aaacactccc acgttataca acatcagata tgggaggatt atacgattac gccccaacag
      961 aagcgataaa agaaaggcct cacttagaaa aacatgaagt aaaagtagga gaatcgtggg
     1021 aaaaagggaa gtataaagct gcagataata acattactta cacagtggca agcaacaaaa
     1081 agcaaaaagt gacagtacca gtatatgctt atttaggtac acaggtaaaa gtaaacggta
     1141 aagtcgtaaa tgactcttat aaaaaacatg gcttagtgaa tgtggatgta aatgctggag
     1201 aaaataagat agaaattaca acaaaatata ctccaactgc tctcatggct ttatggatca
     1261 gtattctgac ttattgttta gtggtgtatt caagttttaa acgatttgca cctaataagt
     1321 tgaaagattc tcgatatgtg actaaaaagg agataaacaa gaagaatgaa aataaataaa
     1381 aaagtagtaa tattgagtgg attaattata gttagtagta tatattcttt catctttttc
     1441 aaaaatacac agagtattta taccactgga gatctttctt ttcacttatc tcgaatcaag
     1501 gggttatcga ctatttttac cagtccgatt aattatgaga cattcaatta tactggatat
     1561 ggtgtaaatt atttttatcc ctttctgact ttttttccag ctgtaatgtt atattggatg
     1621 acaaaaaatt tgattgtaag ctatattatt tacgtttggt tattgaactt gtgtacaaca
     1681 ctcgtagctt atcattatgg cgagcggttt ttaaaacaaa aaaaagcggc atttttattt
     1741 agttgtctct atattttctc tgcttatcgg acagtagaca tctactatcg ttccgcaatt
     1801 gccgaagcta tcgcaataac acttgttatc cctgtattat tttatgctta tcaaatcatc
     1861 tctggaaaag aagaaaagta tccgtctgta aagctagcat tgagtatgag tttgctagtg
     1921 tacagccatg tactatcaac attaatgagt acagcattaa ttatcatttt tatttttatt
     1981 cgtttggttt ctaaaggttt caagaatgca gattttattg ctatatttaa aaaattattt
     2041 agtgcggcag gaatgacatt ggttctgacg agtgcttttt ggtatccgat gtttgaacaa
     2101 atgctctacc aaaaaatcaa caaacctagc gtgacaaatt tatatgctca tgcatctaat
     2161 gtgttcgata gtctgactga agctatgaac aacgacttaa ctacttactc aatggga
//
LOCUS       DACDMM010000241         2181 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDMM010000241 DACDMM010000000
VERSION     DACDMM010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.247"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            59..>2181
                     /gene="acm"
                     /locus_tag="IWD16_002703"
     CDS             59..>2181
                     /gene="acm"
                     /locus_tag="IWD16_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP9089677.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVT"
BASE COUNT          856 a          344 c          425 g          556 t
ORIGIN      
        1 tttttgagag atgatatagt aggttttatt tttagttaaa ataggggaga tagaaaaaat
       61 gaaaaagtgt agaaatatta ttttatcaat gttatttatt attactaata taacatcact
      121 gattcctgtt catgtctatg cggatgcagg cagagatatc agcagtaatg tcacttcgtt
      181 gaccgtggac ccaacgaata ttacagatgg aggaaacata aaagtaaaat tctcgtttga
      241 cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg atcaattggc caagtgaagg
      301 aaatattcgc ggtgaaggat tccaaaaaga aatcccatta atgattgaaa ataaaaatgt
      361 tggaacttta acggtcagaa aagattctgc gcaggttgtg tttaatgaaa atattaaaaa
      421 cctagactca gtagaaggtt gggggcaatt tgagatacag gccagaaacg taaccgatac
      481 cggtgaagaa aatacgggac cattcaccgt gacgagcggt gataaaacag ctactgtaaa
      541 tgtgacaaag ccagcttctg gttcttcttc tagcgttttt tattataaaa caggagacat
      601 gctacctgaa gatactaagc acattcgatg gtttttgaat attaacaata acggcactta
      661 tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt ggacaaagat tagatccaag
      721 tacgtttgaa attaatcaaa tacacttagg tgaacagaag gtttatagag gagaagaggg
      781 aatccaacaa tttttacaag atttccccag cgctacattt aatttttcag taactgataa
      841 ttacattgaa ataactatac caaaaaactt tgtcaatctt aggaaaataa tggtgagtta
      901 taagacgatc atagaaaacc cagaacaaat aaattttgag aatcattcgg aagcttggtt
      961 taaagaattt aataaacctg ctgttgatgg agaaagtttc aatcatacag ttaaaaatat
     1021 ttcagcttct ggtggagtca acggtacagt tagaggcgaa ttgaagattt tcaaatatat
     1081 caatgacaca gagattggta tcccaaatgt gactttcgaa ttaagacgag cagatgaaca
     1141 gcctattcaa ggacagtcta gtatcctgtt gacttcgaat gaacagggag aagctagtat
     1201 caaaggcctg caagtaggag actatgttgt aaaagaaaaa gaagcaccca attggattga
     1261 ttttgatcca ctaagttcaa atgaactcaa attctccata aacgaaaatg atacagaggg
     1321 agtcagttta cctatttata acaagaaaaa ggttacgaac atcacagcaa ctaaaatttg
     1381 gaacggtgga accactccaa gaccgagtat ttattttaaa ttattcagag caagtacaaa
     1441 caattgggaa ccagttcctg atgcagaaac aaaacgatta gacaatggaa taacttcagt
     1501 gacttgggaa gatatccaac agtatgatga tagtggaaac gaatatacgt ttaaagtgca
     1561 agaagtagat caaaacggaa atgattatgt cccttcaggt tatcaaaaaa ttgaaaacgg
     1621 gctagtcgtt acaaatgaga ataaagaagt catttcagtc tcaggacaaa aaacatggga
     1681 agacaatgag aaccaagacg gcaaacgacc gacgacgatc acagtaaatc tattagccga
     1741 cggtcaaccg atccaacaca aagaagtaag tgaaaaagat gattggcgct atcgtttcac
     1801 aaatctgcca aaatacagag acgggcaaga aatcatctat actgtaacgg aagacaacgt
     1861 gcccgaatac agtacgacga ttgacgggta taacatcaaa aacagttata caccggaaaa
     1921 aacaaatata tctataccag gtagccatat aacaccgtgg aaaaattttc cagagaagac
     1981 agagagagag aaaattagaa atctattctc tcaaaaattg caaatatttg ctcatacagg
     2041 agaatccagc gaacaaagaa gcaattatat tagtaagaga gctgtaccta aacagataac
     2101 aaataaatca aaaagcattc ttcctaaaac aagtagtaaa aaatctagtt ttgctgtaat
     2161 cattggttta ttgatagtaa c
//
LOCUS       DACDMM010000242         2135 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDMM010000242 DACDMM010000000
VERSION     DACDMM010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.248"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            202..1572
                     /locus_tag="IWD16_002704"
     CDS             202..1572
                     /locus_tag="IWD16_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAP9089678.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1593..1877
                     /locus_tag="IWD16_002705"
     CDS             1593..1877
                     /locus_tag="IWD16_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089679.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          736 a          311 c          453 g          635 t
ORIGIN      
        1 tgggagtttt gagactacta tatgcgtttt ttgttgattt accaacatgt ttagacattt
       61 tcctctagga aaaaattaac tagcaccacg cgtattgttt atattaaatt agatgaccgt
      121 gataatgcac agcaaatttt tgaaagtatc aattctaccg gtgaaagatt gactgcctcg
      181 gatttgattc gaaacttcat catgatggat aaatctaatg aagagcagac tacgctttat
      241 actaaatatt ggcgtaggtt ggaagaagtc tttgataatt ctaaaggaat ggaagacttt
      301 ttcagatact accttgctgc aataactggg gagtattcag caaagcatat tctttatcaa
      361 gcattcaaag actattggca taaagagcgc gatgtatcaa gtgatgccga attgctccaa
      421 aaattagtgc gatatgctgg ttattttgcg agactttact ataacaaacc tgaaggaaaa
      481 tactcagaag tattgagcga tttccaaagt atggaaagta tgatgcctgc accttttgtt
      541 ttgggattat ctgaatggta ctatcatgac caatttatca ctgaagacca atatattgat
      601 gcgattaagg tagtgaatga ttatcagctt aggagatatt ttaatgggga tgatacaagc
      661 agagtgtcaa aagcatttcc ttcatatttg aaaagtgtta ggaagtacgc taaagctaat
      721 ggatatgaaa atatcgttga tattgtaatt tatgttcttg ttactagaaa tcaatcaaat
      781 aatatggcac ttccatctga caaggcattg aaatctaatc tattgaatgc caatgcttat
      841 gcgatgagat tggcccgttg gttacttgaa aaaatagaga atcgagaaaa ttcagcaaca
      901 cttgatatga gtaacttgtc cattgaacac atcatgccac agactagtac ttcatattgg
      961 gaagagaagg ctggtacatc gggggaagaa tacacaggat tagtaaatac cattggtaat
     1021 ttaacactcg ttacaaatcc tgataattcg gccgcgggaa ataaagactt tgagaccaaa
     1081 aagaaaatct ttgaagatac ccttcatatt cgtatgaata aagatttgta cgaattgact
     1141 gagtggacat catcggatat ttctgccaga agtgaagctc taatcaatga actgattact
     1201 atgtatccct atttgagatc gtcgggcgac tatgaacacg atgggaatag ggaaattttc
     1261 ttagaggcgc aaggaataaa agcaactggg tatttgaacg aagacgagac cgtgattata
     1321 cattcaggtt cagaaattta ttctaagatt aaagatattg catctgattc gctagatgaa
     1381 actcgccaag agctgttgga taatggaatc attgaagaaa caattggggg actacaattt
     1441 gttcaagatt acactgcctc atctgtttcg aatgcagcag ctctgctatt agggggtagc
     1501 cggaatggtt gggactactg gaaatatgat aacggaataa gtattaatga ttcgctaaga
     1561 aataagaaat agagcataga ggtggtgtta ggttggataa gaacaaacta agatatgcaa
     1621 ttcttaaatt gcgtgatgaa aaaaagaatc cttttattga gcttaaaggg caagttccag
     1681 aagaagatat tttagaacaa actaaatttc tttcgcgcaa tggcttacta gaatccatgg
     1741 tttgggcgga taacactgtt catatctggg ggagtgtttc tctggaaggg gaaaaatatt
     1801 tagttgaaaa ctcaggtctt gctaaggcgt atgcattggc aaaagaagtt aaaaattgga
     1861 ttccattttg gggctaagat aagtttttaa acggagaagg tagcaaatca taattggttt
     1921 gttgcctttt ttgattgccc aaaaagaaaa atgacaaagt atagagataa atgacatagt
     1981 acacgggaaa aatgacattg tatcaccgac aacgtctaaa tagatgttga tatatatacg
     2041 ttccgtacaa ataattataa aaatttaaat gatttgagta gaagtatcag tgaatatctc
     2101 aaggggaata gctatctcaa gattcgattt tcaca
//
LOCUS       DACDMM010000243         2068 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDMM010000243 DACDMM010000000
VERSION     DACDMM010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.249"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IWD16_002706"
     CDS             <1..177
                     /locus_tag="IWD16_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089680.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IWD16_002707"
     CDS             190..690
                     /locus_tag="IWD16_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9089681.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..>2068
                     /locus_tag="IWD16_002708"
     CDS             787..>2068
                     /locus_tag="IWD16_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP9089682.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEIS"
BASE COUNT          669 a          281 c          464 g          654 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaa
//
LOCUS       DACDMM010000244         2058 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDMM010000244 DACDMM010000000
VERSION     DACDMM010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.250"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..337)
                     /locus_tag="IWD16_002709"
     CDS             complement(<1..337)
                     /locus_tag="IWD16_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9089683.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="IWD16_002710"
     CDS             complement(404..559)
                     /locus_tag="IWD16_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9089684.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="IWD16_002711"
     CDS             1176..1397
                     /locus_tag="IWD16_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089685.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="IWD16_002712"
     CDS             1398..1655
                     /locus_tag="IWD16_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089686.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="IWD16_002713"
     CDS             complement(1791..>2058)
                     /locus_tag="IWD16_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9089687.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DACDMM010000245         2045 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDMM010000245 DACDMM010000000
VERSION     DACDMM010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.251"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            108..797
                     /locus_tag="IWD16_002714"
     CDS             108..797
                     /locus_tag="IWD16_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9089688.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            811..>2045
                     /locus_tag="IWD16_002715"
     CDS             811..>2045
                     /locus_tag="IWD16_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9089689.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSE"
BASE COUNT          635 a          280 c          413 g          717 t
ORIGIN      
        1 aaaactccta aatactccat ttttatcttt acttttatga aagcggatgc tatattgttt
       61 ttggagttgt tatccaaaaa taataaaaaa ttggagtgtt taggataatg gataaaaaga
      121 ggtttttaga tcttatttca aaaggtttga ttgtttcctg tcaagcatta cctggtgaac
      181 cattttacaa agaggaagaa agtttaatgc cttattttgc tttggctgct caaagggctg
      241 gagcagttgg tatacgtgca aattctgttc gagatattat tgaaattaga aagtgtgtat
      301 cattaccaat gattggaatt attaaaagag attatccacc agaatctcca ttcattactg
      361 ccactatgag agaagtagat gaattagtag aaaccggtgt tgaggtaatc gctcttgatt
      421 gcactcttag aaatcgacat gatggactat ctataaattc ttttattact aaaataaaag
      481 aaaaatatcc tcatcagtta tttatggcag atatttctac atttgaagaa ggcaaaaatg
      541 catataaagc aggaatagat tttgtaggaa ctactttaag tggatatact gaggaaagta
      601 aaaaacaaga tgggccagat atagatttga ttaaaaaatt ggtagaagaa gggatttgtg
      661 taattgctga gggaaggatt catactccag aacaagcaaa agagataaaa aaaataggag
      721 ttgcaggtat tgttgtgggt ggtgctatta ctcgaccaca ggaaattgca tcaagatttg
      781 ttaaagcatt aagttagggg gagaaataaa atgtttaaaa aattttcaca attaggacgc
      841 gctttcatgt taccaattgc tatattacca gtagccggat tactattggg actgggagga
      901 gcgttaacaa atgagagtgc tataaatgct tatccatttt tagatcaacc gtggttgcat
      961 acaattttaa gtattatgag ttatgctgga aatgcagttt ttgcaaacct agcactaatt
     1021 tttgcaattg gtattgctgt aggacttgca aatggagata aaggaacagc tggtttagct
     1081 ggtggagtag cttatctagt atatactgca actattagtg ggtttcttga acttttttca
     1141 gcaaaagatg caacactaga tacaggtgta gtgggatcaa ttgttgttgg tggtactgtt
     1201 gcatttttac acaatcgtta tagaaaaatt gaattaccac aattcttagg tttttttgga
     1261 ggatcaagat ttattccgat tgtttctagt ttagctgcaa tcattattgg ttctttcttt
     1321 tatttaattt ggccaccaat tcaaaatggt ttagtagttg ctggtgaaca aattgcccaa
     1381 atgggtagtt taggaacttt tttatatggt tttttattgc gtttaacagg tgctgttgga
     1441 ctacaccaca ccatttaccc tttgttctgg tatacatctt taggtggtac cgaaactgta
     1501 gctggatcaa caattgctgg agcccaaaat attttctttg cccaactagc tgatccaaac
     1561 cacactggtc tgtttactta tggtactaga ttttttgcag gaagatttgc aacaatgatg
     1621 tttggattac cagcagcatg ttatgcaatg tatcgtgcaa ttcctaagaa aaatcgtaaa
     1681 aagaatggtg ggctttattt tagcggggcc cttacatcat ttttaactgg aattactgaa
     1741 ccagttgaat atatgttttt atttgttgct ccttggcttt atgtgattca tgcattctta
     1801 gatggattat ctttttattt tgctgatatc ttaaatattc gtgtagggaa ttccttttca
     1861 ggtggtttga tcgacttttt attattcggt gtacttcaag ggaatgacaa aactaattgg
     1921 ttaaaattaa ttccttttgg tattgcatgg gcatttatat atttctttgt ttttacattc
     1981 tttatcaaaa aatttaaggt agctattcct ggaatggaag aagaatcaga agttcattct
     2041 gaaaa
//
LOCUS       DACDMM010000246         2034 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDMM010000246 DACDMM010000000
VERSION     DACDMM010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.252"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            17..457
                     /locus_tag="IWD16_002716"
     CDS             17..457
                     /locus_tag="IWD16_002716"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9089690.1"
                     /translation="MVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEILVGDII
                     TYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGL
                     VLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            585..1268
                     /locus_tag="IWD16_002717"
     CDS             585..1268
                     /locus_tag="IWD16_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP9089691.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1577..1650
                     /locus_tag="IWD16_002718"
     tRNA            1577..1650
                     /locus_tag="IWD16_002718"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1611..1613,aa:Arg,seq:tct)
     gene            1809..>2034
                     /locus_tag="IWD16_002719"
     CDS             1809..>2034
                     /locus_tag="IWD16_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP9089692.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSA"
BASE COUNT          679 a          322 c          422 g          611 t
ORIGIN      
        1 tattcttctc gtcgtcttgg ttccccgaat ttttggtttg actccttata ttattgagga
       61 caaagaaatg agtccatatt atgcacaagg aaatctgata tatgtacgac cgaaaaatac
      121 aaatgagatt ttagtcggag atattattac atattatgaa aattcaggaa aaaaaatctc
      181 gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat ttttatacaa aagctgatac
      241 aaagacttat attgagcctg gagtagtaaa aaagagaaat attatcggta gtcccatctt
      301 tcagattcca tatattggac tagtcctttc aaaaacggca tttgcatgga ttcaggggtt
      361 attctttacg attgcttttt gcctaacagg aatcacagca tggaatacct ttgtagagtt
      421 gaaaaagaag cggagacgaa cacagaaatt tttgtaagaa tgttaaaaac ataaatgtat
      481 tccagagatc caacgactct tctgaacatg aaacacgaga aaacactcat gcagcttgta
      541 gatttagtca gttgttcgta taatgtgtga agaggaggag tagtatgcgc aatgaaatga
      601 tgcatcgtcc tgtagtcaaa gaagaactat tggattttat gcgtacccag caaaaaaaat
      661 taccgggtga gctaggcaaa ctggaagaag aagctcatgt agcagaagta ccagttattc
      721 cccatgaaac agttgtattt ttgaaatttt tacttggaca gcttcagcct gaacgaatct
      781 tggagatcgg agcagctatc ggtttttcat caagtgtcat ggccacgaca atcggagaaa
      841 acgctcatgt tacgacaatt gatcgtttcg atgtcatgat cgagaaagct aaaaaaaact
      901 atgaacgtct aggactgaca gataaagtga ctttgcttga aggtcaagca gcagatatct
      961 tgccggagtt gtctggtccg tatgacttta tttttatgga tagtgcaaaa tcaaaatata
     1021 ttgaattttt acctgaatgc ttacgtttgt taagaaaagg tggcgttttg atggtagatg
     1081 acatcttcca aggaggaacg atcttgctcc cagatgaaga gattccccgt ggcaaacgag
     1141 ctatccatcg taaattaaat gaatttcttc gtgttgtgat ggatcatccc gatttgacat
     1201 cgacaatctt gccattaggt gatggtgtga tcttaatgac aaaggaatca gaaacaatcg
     1261 agttgtgata catgattatg gcagcctgta gataatcgat tgaatcgagg gctcaattcg
     1321 aacttgatgt aaaagtttct atatttgtaa gtcaattaaa ggatacagga aatctaaata
     1381 aacaggagta gcatgaagtc aatgtagctc ctgttttcaa ttgataacct tagaacagct
     1441 gaaggagaat gtctcgtttt aaaagattga tttagtagtt gggccaaaat aattcattat
     1501 ttgctaatta aataactatt tcgtattgac agaagcaatt taattatcta taattattga
     1561 tgttccgctg attcttgtca tagtagctca gctggataga gcatccgcct tctaagcgga
     1621 cggtcggggg ttcgaatccc tcctgtgacg ctaaaaaacc gttagaaact gaggtttcta
     1681 acggtttttc tttgtttttt gtgccaatat tgtttgtttt caaaggcaaa taaaataaaa
     1741 aaacatcctt atcgagtaca attataagga gcaaaaaaat aaaactcatc agaaaagagg
     1801 aagaagatat gacgatacta tctagaattt tagtgactct agttgcaatt gaattttttt
     1861 atatcatgta tatcgaaacg gtgagaactg attctgacac gacaagtagg gtttttaaaa
     1921 tgagtaaaga agagttaagc agaaaatctg ttcaaacatt gtttaagaac caaggagtgt
     1981 acaatggact attaggagtg ggactacttt atggagctta tctttcttcc gcat
//
LOCUS       DACDMM010000247         2011 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDMM010000247 DACDMM010000000
VERSION     DACDMM010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.253"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            27..1640
                     /locus_tag="IWD16_002720"
     CDS             27..1640
                     /locus_tag="IWD16_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP9089693.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          641 a          355 c          413 g          602 t
ORIGIN      
        1 atcaacgtag aaaaggagat gtttttatga aagttgttgt cattggcggt gtagctggcg
       61 gtccttcatt tgccactcgt ttccgtcgat tgaatgaagc gcacgaaatc attatctatg
      121 aacgcggaga aaatatttct tatgcaagtt gtgctttgcc ttattattta ggaggtgtga
      181 tcactgaccg tgactcgctg atcgaacgta caccagaaat attgaaaaca aaaaacaaca
      241 tcgatgtctt tactaaacac gaagtaacag caatcgatcc ttctactaag cgattaacag
      301 ttaaagacct atccacaaat gaagaaataa aaacagatta cgataagttg atcatctctt
      361 ctggtgctag accagattat ccggatattc ccggagtttt tgaagcagaa aacggctttg
      421 tcctccgtag tgtgacggat gcggatcgaa tcaaatcgtt ccttgaagaa aaaaatccac
      481 aacatgtcgt cattcttggt gcaggtgtta tgggtctgga attagctgag aatttcaagc
      541 atcgcggctt agacgtgact ttaatcgatc aattgccaca agtcgctttc ccttatgatc
      601 cagaaattgc taatttagtt tatgacaaat tgctgaaaga aggattagcc gttcatttag
      661 aaacaagagt tactgagatc cgtgataaag gtcgagaaat agtattatca gatggctccg
      721 tcctttctgc tgatatgcta atttttgctg ttggtgtttc tccgaagaat gaagtggtga
      781 aagcaaccgg catacaatta tcagatacag gtcagatcat tgttgatgac cagttacaaa
      841 ccaatctgcc ggacatctat gcgattggcg atattatcga aacgactagt gtagtgactg
      901 gtcagccgat ccaaagtatg ctttccagtg cggccaatcg tcaaggacat atgctggcag
      961 atattttaaa tggtacgcct atgcgctatc gcggatatat tggtgcaggt gtcgcaaaaa
     1021 tctttgatca tacagcaagt tatgcgggaa tgacagaaca tgaattaaaa gcatcaggca
     1081 taacaaatta taaaactgtt tttatcactc cctttgacca tgcctatttc tatccagaag
     1141 ctacaagatt aaatctaaag ctgatttttg atgcagatag cggtcgtatt ttaggtggac
     1201 aagcatttgg agaaaaaggt gtcgataaac ggatgggaga actttctgta gcaatcaccg
     1261 gaaacttgac agtctttgat ttgcctgatt tggagttgcc ttactctcca ccgtattcta
     1321 ctacccgtga cccgttgaat atagctggtt atgtcgcaat caatcaaatg acgaatatcg
     1381 tagaaacgat caaagcaagt gatatacccg aaaacgattt gaaagaagcg ttctttttag
     1441 atatacgtga acctaataaa gcaccttctg gcagtatctc ggcaacaaaa aatattccaa
     1501 tgaatgagtt acgagatcga atcaatgaaa tcccaaaaga taaaaaagtt tacattactt
     1561 tcagaagagg attgaatact tatacttctg cccgaatctt ggcaggtttg ggtatcaaag
     1621 cgattttgat tgaagaataa aaaaacgacc gcggtgggta gccgcggtct aggagttatt
     1681 tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat gtatatagaa
     1741 tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg ttatagttta
     1801 agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat aaaaaaacat
     1861 gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac tacgcctaat
     1921 attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc cctttcgaat
     1981 attttttctt atataataaa gtttgctttc a
//
LOCUS       DACDMM010000248         2003 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDMM010000248 DACDMM010000000
VERSION     DACDMM010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.254"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IWD16_002721"
     CDS             <1..378
                     /locus_tag="IWD16_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9089694.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IWD16_002722"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IWD16_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9089695.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="IWD16_002723"
     CDS             1859..>2003
                     /locus_tag="IWD16_002723"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9089696.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DACDMM010000249         1976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDMM010000249 DACDMM010000000
VERSION     DACDMM010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.255"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IWD16_002724"
     CDS             complement(<1..914)
                     /locus_tag="IWD16_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP9089697.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IWD16_002725"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IWD16_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP9089698.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          653 a          365 c          386 g          572 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcct
//
LOCUS       DACDMM010000250         1949 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDMM010000250 DACDMM010000000
VERSION     DACDMM010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.256"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..425)
                     /locus_tag="IWD16_002726"
     CDS             complement(<1..425)
                     /locus_tag="IWD16_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9089699.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="IWD16_002727"
     CDS             complement(534..1565)
                     /locus_tag="IWD16_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9089700.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          601 a          322 c          338 g          688 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttg
//
LOCUS       DACDMM010000251         1946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDMM010000251 DACDMM010000000
VERSION     DACDMM010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.257"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            592..1920
                     /locus_tag="IWD16_002728"
     CDS             592..1920
                     /locus_tag="IWD16_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9089701.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
BASE COUNT          653 a          320 c          383 g          590 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgta
//
LOCUS       DACDMM010000252         1929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDMM010000252 DACDMM010000000
VERSION     DACDMM010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.258"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IWD16_002729"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IWD16_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9089702.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IWD16_002730"
     CDS             852..1040
                     /locus_tag="IWD16_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9089703.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IWD16_002731"
     CDS             1053..1610
                     /locus_tag="IWD16_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9089704.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IWD16_002732"
     CDS             1642..1884
                     /locus_tag="IWD16_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9089705.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          710 a          296 c          397 g          526 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgag
//
LOCUS       DACDMM010000253         1900 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDMM010000253 DACDMM010000000
VERSION     DACDMM010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.259"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(589..954)
                     /locus_tag="IWD16_002733"
     CDS             complement(589..954)
                     /locus_tag="IWD16_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089706.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1030..1275)
                     /locus_tag="IWD16_002734"
     CDS             complement(1030..1275)
                     /locus_tag="IWD16_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9089707.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1345..1599)
                     /locus_tag="IWD16_002735"
     CDS             complement(1345..1599)
                     /locus_tag="IWD16_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089708.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
BASE COUNT          697 a          323 c          339 g          541 t
ORIGIN      
        1 ttttttaaag aatatttaga aattattaat tttcgtctga aaacaaaaaa agaaagcgtt
       61 attattttag tctattttca gggggggaac cttatgataa aaaaactatt tttactatca
      121 atattaacgc tagcaatggc aggttgttct gactctggct tagcaactga actgatcaca
      181 aaatcaactg aagccacgac agaggagtta aaatgtggaa acgatcacag tgactacaga
      241 ggcttaaatc gacgatgcat gggaatatta ggcagaagca attgtaagca tcgatgtagt
      301 aatcgaagaa ggtgtagaag aagcaacaga aaaatcaaaa gaaagatcaa aagaagcaat
      361 ctgtgctgtg catcgtatac agacgtcgaa gatgcggtac ttttaaacca cgtcaggaga
      421 tgttcattta tgtctaggga agttggcgat gaaccaaaac tagagtacgc ttttagtttt
      481 gcacgtgagg atgaagataa ccctaaaact tacgacattt tagcttgatc taagatggtt
      541 aaagcttttt aatgaagtaa gaggagatag aaggcttttc tagttatatt aggtaagata
      601 aattcctttt tgctccaaat ttgctaaaca ttctgctaga taaacttcat cgtgttccgg
      661 ataaatcggc acgttgcgct ctacagtagg taaatcaaaa aatgcttcgc atgatttgcc
      721 acgatacata ggatttttcc actctggtgc tgggctgtaa ccacgttttt ccatttcatc
      781 catgacaagc tgatgatatt ggaataatct ataaggagaa tagtgaaaaa cataatccac
      841 agtagcatgt ttctttcccc agccatttcc tcttaatgca cagcattccc gatgttgtcc
      901 taaaagctgt tgacgaggaa gctttgaaat caatttttca tgccataatc gcatagaaat
      961 gtctccttta tatatagatt gttaacctta gacgttaaat gaaaaaacca gcaccaaggc
     1021 tggaaaaaat cacttcagtt tgcttgctgt ttctgagcga ccaattaggt aatcaacgga
     1081 aatatcgtaa aagtcagcga tatagattaa ttcaatgatg cttggaattt cttctccttc
     1141 ttcgtactta cgatatttga ataaaggaat acttaacgcg ctagaaacag ctacttgaga
     1201 taaagatcgg ccagttctta agaaattcag acgacaacgt aaagtatcca gtaaaatagc
     1261 ttcttcgata ctcatttaat cacctttcat tatagtagaa tagttataat gactatagaa
     1321 agctacacat gcaatcaata gtactcaatg ttttttattt aaacgtatca aataaagaat
     1381 gacgatcaat aaagacaaaa ccagtcctga taattcttct ataaaaacac tatcgggttc
     1441 aaagtagttg taattaaaaa ctttccacat aaagtagata gccggaagag tcaaaatagc
     1501 taaataaaac cgttctttgt tccaattcat aagataaaca atacttgcga ccagtgcgac
     1561 cgcaggcacc caaaacaaaa ctctcaccaa cgtaatcatg taacggactc ctcaacgatt
     1621 catataataa aagataacat gttattgcat acatcacaaa tgaaatgcta ggaaaataaa
     1681 agaagaatag atcaatgaaa aatgaaaaag cagaacagtt ttctgctgat aagcaggaac
     1741 tgttccgctg aatgtgtctt atctatcgag aaaaaatcaa tggattgata tctaagtaat
     1801 tcaatacata taaaaagagg acgatcgcat tcactaaaaa ggaaagaatg atgatcccat
     1861 agcgaatgga aacatattta acaaatactt ttgtttgaat
//
LOCUS       DACDMM010000254         1891 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDMM010000254 DACDMM010000000
VERSION     DACDMM010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.260"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            173..1309
                     /locus_tag="IWD16_002736"
     CDS             173..1309
                     /locus_tag="IWD16_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9089709.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="IWD16_002737"
     CDS             1446..1790
                     /locus_tag="IWD16_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP9089710.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT          655 a          338 c          400 g          498 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat t
//
LOCUS       DACDMM010000255         1855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDMM010000255 DACDMM010000000
VERSION     DACDMM010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.261"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            64..816
                     /locus_tag="IWD16_002738"
     CDS             64..816
                     /locus_tag="IWD16_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9089711.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            821..1768
                     /locus_tag="IWD16_002739"
     CDS             821..1768
                     /locus_tag="IWD16_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9089712.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          643 a          254 c          369 g          589 t
ORIGIN      
        1 aaagaaaact ggaatgattt atatcatgca aacgaagtat tgaaaatagc tgaggagaaa
       61 taaatggaaa ttttatttac tatttgtggt agagctgggt ctaaggggtt taaaaataaa
      121 aatttgaaat atttggcagg aaagccccta gtccatttcg ctttagcaat gatagatatc
      181 tatcaacaaa aacatcctga acataattgc acgacttgtt tgaatacgga cagtaatgag
      241 ttagtagaat tgacgactaa gttaaatccg tctgtgaaaa ttgtagaacg taaacctgaa
      301 ttagctacag atactgtagg gaaaattgaa gtgatacgtg attcattctt acaactagaa
      361 agagaagata atatatttga tttaattata gatttagatt tgacttcacc tttacgtgaa
      421 gtttctaatc ttgaagaatt aatagatgaa catctaaaaa atttagataa agacgtctac
      481 ttttcagttg tttctgcaag acgtaatccc tattttaata tggtaaaaag aattgaaaat
      541 gaagttacta tagttaataa atcaagatac acatctaggc aacagacacc tgaagttttt
      601 gaaatgaatg cctcaatgta ttcttttaaa cctgaatttc taaaaaaaag taattatatt
      661 tttgatgggc aatgtgggat aataaaaatg agagattatc taattttaga tattgatagt
      721 gaagaagatt ttaaatggtt agaatttttg tttcctaaat ttttagaaga ttcttcggag
      781 cttcaagata tctataatta tttatatcgg aggtaaaagg atgagctgtt atcttatcac
      841 cggtggggct ggcttcattg gttctacctt agccaatcat ttaggagaag aacatgtggt
      901 aatcgtcgtc gatgacttat caatgggaaa agtagaaaat cttgatatga cgaaaaatat
      961 cacttttatt gagggagatg tcgctgagaa atccttgatg gaagagatca tgaaagctaa
     1021 taaatttgat tatatctttc atttagcagc agttgctagt gtggcagatt cagttgcccg
     1081 accagtagaa acacatcgtg tgaattttga aagtgtatta atgattttag aattgatccg
     1141 caagtatcaa cctgatttaa aacggatcgt gtttagttct tcagcggctg tttatgggga
     1201 tgaaccgact ttaccaaaaa aagaagagtc cgtgattcgc ccattgacgc cttatgcggt
     1261 tgataaattc gctgcagaac aatatgtgct agattattgt catttatatg atgtacccgg
     1321 tagcgcagtt cgttttttca atgtctatgg tccaaatcaa aatccgaatt ctccatacag
     1381 tggtgtgatt tctattctcg tagatcgcta taagaaacaa atcgtaggag aagcaacttc
     1441 atttacatta tttggtgatg gaagccagtc tagagacttt gtttatatag atgatgtaat
     1501 ccaagcatta cttttagttg cgaatgagga agctgcactg gggaaacaat tcaatgttgg
     1561 aacgggtaaa gcaaccacac ttttggaatt gattcatgca atcaatgaaa ttctcggtgt
     1621 agaattgaca cttgaataca aagaagagcg ttcgggtgat atccatgact ctctagcaga
     1681 tattactaag atccagtcaa ttggctatca accgaaatat ggtattttaa gtggaatgac
     1741 taattatttg aaaactgaaa tcaattaaaa catactaaga taagattaga gggtaaattc
     1801 aggctctttg tcaataagga ctgatgagtt ttgtaaaatc aaatctggga tagaa
//
LOCUS       DACDMM010000256         1854 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDMM010000256 DACDMM010000000
VERSION     DACDMM010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.262"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(365..1529)
                     /locus_tag="IWD16_002740"
                     /pseudo
     CDS             complement(365..1529)
                     /locus_tag="IWD16_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343665.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          632 a          334 c          282 g          606 t
ORIGIN      
        1 tttctcagaa acaaaccact cattggaaaa gacatgaatc aaatcatcac ttacttgtgt
       61 aaaatagcga cgtttaatga caaagatatc ttcattgcta tctagtgtac cttcatttct
      121 ttgtgcttaa attcaaagat aattttctca ataaagattt ttgattccag taagattcct
      181 ttactccttt tacaacatag cgttgtcctt tgttttgaaa catcttcttc ccctctttgc
      241 cattaaaaat agcccaattc tctataaaga agattaagct tcattattat aatataaagt
      301 ttaaatttgg tttattactt actttttctt attctctagt aagctaatat tgaaaaaaat
      361 gtaatcagat cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa
      421 agaggtaact acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag
      481 aaacttcaat gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc
      541 tctctttttt gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt
      601 attttttgtc ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa
      661 ggcatagtat gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat
      721 tgtaatgctt ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga
      781 ataagatttt cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag
      841 tttgcttttg ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact
      901 acttttcttt gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca
      961 aacagtactt gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt
     1021 tgtaataaag accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg
     1081 taaatcactt tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa
     1141 ataataccaa tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa
     1201 ctatccgaga catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat
     1261 tgaaccatga tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt
     1321 cctactgcct ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt
     1381 actacaaagc cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga
     1441 acatgtgtat aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc
     1501 tcataaatca gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct
     1561 tcagcaatag cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa
     1621 cagctaaata taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc
     1681 agcatgatag tacaaacatt caatagccaa acatataaga tataactaac gattaaatta
     1741 ttacttatcc aataaaaaat gactgccgga aaaaaggtca agtagggata aaaataattc
     1801 actccgctcc cataatgatt aaacgtcgta aaattgattg gaccagaaaa aatt
//
LOCUS       DACDMM010000257         1835 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDMM010000257 DACDMM010000000
VERSION     DACDMM010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.263"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(279..734)
                     /gene="tnpA"
                     /locus_tag="IWD16_002741"
     CDS             complement(279..734)
                     /gene="tnpA"
                     /locus_tag="IWD16_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP9089713.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            977..1835
                     /locus_tag="IWD16_002742"
                     /pseudo
     CDS             977..1835
                     /locus_tag="IWD16_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          581 a          357 c          346 g          551 t
ORIGIN      
        1 agattatttc tgcgccagca ttttctaatg tttgaatttg tgttttgtaa tcttggttgc
       61 ttgtgctaat tcttgaatag ccgtaaattg ccaaaatttc atcacccttt ctggacataa
      121 gtttctgaca ccagttaaaa tcaagtctat cagtaaccca tacacccgtg aggacgggtg
      181 tatttttaat ccccctagaa ggggtcgaca ctcgctgccc cttaaaaggg gatttttaaa
      241 agaccgccaa ctgctcatct tcactcactt acttctcttt attttttaaa tggttctacg
      301 tactctcttt tacttataat atatcgaatg cgatcatcag cttcttgttc acggatgtat
      361 ttttcatttg tcttttgatt caatccgact gtactcacat agtatctctt cgaccaaaag
      421 gatttgtttc cgtatttata tttgagattt gcgtgctgct cgtggattag taccgcacta
      481 cgacctttca gataccccat aaaactggat atacatattt ttggtggtac tcgaacaagc
      541 atgtgaatat gatctagcat tgcatgtgct tctatgattt ccacatcttt catttcgcac
      601 aacttcctaa gtatttttcc aagatctttt cgtaattgcc catagattac ttttcgttga
      661 tattttggtg taaaaataat atgatactta caattccaac gagtatatgc taaactttta
      721 tcgtcatttg acatgaaaaa actcctttcg atctgatgag cggttggtag ccactcacta
      781 gtatatcgaa aggagttttt atcgctaaag atacttcttc cacgcgtaga aacgcgcggt
      841 tttcttgttt cgcacgcttt acgcacaaaa ctgactgaag tcattaagaa aataaaaggg
      901 tctaaaactt ttaagttttg taccgacccc caaaagttag accaaaaaat ctaacttttg
      961 gaggtcggta ttttttatgg ctaagtatac ttttgaactt aaattaaaga tcgttcatga
     1021 ttacttggat ggtaaaggcg gaagtgatta tctggcaaag aaatattcca ttaaggctcc
     1081 atcacaagtg aagcgctgga tcaatgccta tcaagaattt ggtgaagagg gtctagtaag
     1141 aaagcgacaa aagaaaaaat attctgttca atttaagctg gatgcgatag agttatatct
     1201 aacaagtgag ctgtcctatc gtgaagttgc caacacactt aacatgaaca accctaattt
     1261 gatcgctagt tggatgcgtc aattccgtga gggtgggatc gatggactct caaaaacgaa
     1321 aggatgtcca cctatcttgt ctaagaaaaa tgaacccaaa aataagaaaa aatctacaac
     1381 taaagcaacg tctaaagaac gtgaacgcat cgaggagttg gagaaacgag tccgttccct
     1441 tcaaattgaa aatgcctttt taaaagaatt gaggaagctg cgaaaacagg aagcccaaca
     1501 acgacgaatg aagcaatcgc acgaatcatc gcaagcctcc gaggaccgtt taaattagtt
     1561 gagcttctag aaactttaaa gttccctaag gcaatcttta tgtactggca aaaacgattg
     1621 gaccgtaaaa atccagatca agagattgaa gcggaaatgc ttaaagttcg ggagaagcac
     1681 aaggattatg gctgtttacg gatgaccaat gaactaagaa accgcggttt ctctatcaat
     1741 aaaaagaaag ttcagcgcct gatcaagaag ctaggaataa aagtaacgac atacacacgt
     1801 aaatcacggc gctacaattc ttatcgagga caagt
//
LOCUS       DACDMM010000258         1815 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDMM010000258 DACDMM010000000
VERSION     DACDMM010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.264"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(243..464)
                     /locus_tag="IWD16_002743"
     CDS             complement(243..464)
                     /locus_tag="IWD16_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089714.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="IWD16_002744"
     CDS             complement(562..807)
                     /locus_tag="IWD16_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089715.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="IWD16_002745"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="IWD16_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          606 a          319 c          270 g          620 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcat
//
LOCUS       DACDMM010000259         1803 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDMM010000259 DACDMM010000000
VERSION     DACDMM010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.265"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IWD16_002746"
     CDS             5..1150
                     /locus_tag="IWD16_002746"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP9089716.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IWD16_002747"
     CDS             1321..1779
                     /locus_tag="IWD16_002747"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP9089717.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
BASE COUNT          587 a          346 c          363 g          507 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gag
//
LOCUS       DACDMM010000260         1800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDMM010000260 DACDMM010000000
VERSION     DACDMM010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.266"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            44..>1800
                     /locus_tag="IWD16_002748"
     CDS             44..>1800
                     /locus_tag="IWD16_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP9089718.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDT"
BASE COUNT          654 a          228 c          361 g          557 t
ORIGIN      
        1 tactttttgg aaaaatctta ctaataagta gaggagaaag aaaatgacta taaaagtaga
       61 cttacgaaaa aaaccatatc atttagatga agatgctatt tcttggattg aagagacgat
      121 tgatgctatg actttagaag aaaaaatagg tcaattattt gttaatatgg gatctagtcg
      181 tgataaaaat tacttaagta gtatggtcaa tcagtaccat attggagctg tacgatataa
      241 tccaggaact gctgatgagg tatatgatca aaactatatc ctacaaacag aaagcaaaat
      301 tcctttatta attgctgcta atacagaagc aggtggtaac ggagcttgta cagatggtac
      361 cgaagttggt gtagaagtaa aaattggtgc aacaaatgat gcgaaatatg cttatgaatt
      421 agggagagtt tctggaattg aagcatcggc tattggatgt aactggagtt ttgctcctat
      481 tgtagatatt aatagaaatt ggagaaatcc tatcatttct agtcgatctt ttggacaaag
      541 ccctgataaa gttttagaac ttagtttagc ttatatgaaa ggtattcaag agagtggaat
      601 tttgcctgca gctaaacatt tccctggtga cggtattgac gaacgagatc aacatctatc
      661 ttttagtatt aatagtttaa gtacagaaga atgggataat tcttttggaa aagtatactc
      721 aggcttaata gaagctggat taccttcaat tatggcaggt catatccata tgccagctta
      781 ttcaaaacat tttaatcttg atctgaaaga tgaagattta cttcctgcaa ctttatcaaa
      841 agaattaatc actgacttac ttagaggaaa actcgggttt aatggattag tagttactga
      901 tgctagtcat atgttaggaa tgactggggc aatgaaacgg tcagaattat tgcccacttc
      961 tattgcggct ggatgtgatc tattcctatt ttttaatgat ccagatgaag actttaatta
     1021 tatgatggaa ggttattaca aaggaattat ttcagaagag cgtttacgtg atgcattaag
     1081 aagaatttta ggcttaaagg ctaaactagg tttgcataaa aaagaaaaga ataaaatttt
     1141 gttaccgaaa caagaagcta tggaaaaaat tggtttaatt gaaaataaat caatttttaa
     1201 agaggtagct gatcaagcaa ttacactagt taaagataaa caagatattt ggcctattgt
     1261 accagaaaaa tataaaagga ttttactggt agatgtacat ggggtaaaag gtgggtttgg
     1321 tgaagtagtt gctggaaata gacctcaggc cattgattta ttgaaacaga ttttgtcagc
     1381 aaaaggattt gaagttgaaa aatacatttc acctgaagaa gaattgaaag gattatcgca
     1441 agaagaaaaa attaaaaata tagctggaat atatgctgct aagagaccaa tcaaagattt
     1501 aactgaaaaa tatgacttga ttattaatat agctaatgtt aacccaggta cagttcaaag
     1561 aattgtttgg ccagcaagta aaggtactcc ggatattcca ttttatattc atgaaattcc
     1621 aacaattttt gtatctgtac agtgtccgtt tcatttagct gatgtgcctc aagtaaaaac
     1681 ttatatcaat acttatgatg gaaaacaaga aactttagaa atattagtgg acaaactaat
     1741 gggcctttct gaatttaagg gaataagtcc aatagatgct tattgtgggt taaaggatac
//
LOCUS       DACDMM010000261         1799 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.267, whole genome shotgun sequence.
ACCESSION   DACDMM010000261 DACDMM010000000
VERSION     DACDMM010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.267"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            119..1045
                     /gene="cysK"
                     /locus_tag="IWD16_002749"
     CDS             119..1045
                     /gene="cysK"
                     /locus_tag="IWD16_002749"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP9089719.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1281..1727
                     /locus_tag="IWD16_002750"
     CDS             1281..1727
                     /locus_tag="IWD16_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9089720.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
BASE COUNT          614 a          271 c          401 g          513 t
ORIGIN      
        1 attaccatat atggtatatc tttgtctttt ttttgctagt caaagacgaa tcactcgcca
       61 taaatgaaca gattgtatta tgataagtaa gaagagcaaa gacaaggagg aattgaagat
      121 gacagctatc tataactcag tcaccgaatt gattggcaaa acgccaatcg tcaaattaaa
      181 taaaatcgta ccagaagatt cagcagatgt ttttgtaaag cttgagtttt ttaatccagg
      241 aggaagtgta aaagatcgta ttgctttaag tatgatagaa aaagcagaac acgatggtct
      301 actgaaacca ggtgatacta tcattgaacc tacttctgga aatacaggaa tcggattgtc
      361 gatggtagga gttgcaaaag gatataaagt aatcatcgtg atgcctgaga cgatgagtat
      421 cgaaagacgt ttattgatga aagggtacgg cgcagagttg attttgactc ctggtgcgga
      481 tggtatttct ggatcaatca gagaagcaga acgtttggca aaagaaaacg gctacttttt
      541 accattacaa tttgaaaatg aagcaaatcc tcttgtgcat gagaagacga ctggtcctga
      601 gatacatcaa gcatttggag taaatggctt ggacgcgttt gttgcaggta tcggtacagg
      661 aggaacgatc actggcgctg gccgagaatt aaaacgtgtg tatccgaaaa tcgaattaat
      721 cggggtagaa ccagcagaat ctgctatttt agaagggaaa gaagcgggac ctcacaaaat
      781 ccaaggaatc ggtacaggtt ttgttcctaa aacactggat acttctgtgt atgataaagt
      841 cctttctatt agtggagacg aagcgatgga aacagctaga gaagttgggc gaaaagaagg
      901 gattttagta ggcatctcat ctggcgctgc aattgctgcc gcgctaaaag tcgcaaaaga
      961 attaggaaaa ggcaagaagg tattagctgt ggttcccgac aatggtgaac gttatttatc
     1021 tacagccctt taccaagaga tataaggcac ttggtttttc tccagatagg tttatgtaaa
     1081 aaacttttaa agatgaaaag aacacacaaa aatttatgat gagaaaaatt atttgaatag
     1141 agacgtagaa tagtgaacgc gactgtttta cgtctttttt cattaaaata caataaaatt
     1201 gaattttctt ttcatgttaa gtggatttta aaatgtaaaa atagtatgat aagacgagaa
     1261 taacaacgtg gggtgtttac atggaaaata agttagtcga agaggttatc gaaaagttaa
     1321 aaaaagagaa cattcgaatc acacctcaac ggtacgcggt tttagaatat ttgattgaac
     1381 atcattccca tccaacggct gatgagattt ataaagcact tgaacatcgg tttccaaata
     1441 tgagtgttgc gactgtttac aataaccttc gcttatttac ttcgattgga tttgttcaag
     1501 aaatgaagta cggcgatgct tctagccgat ttgatttcag ttcaaagcgt cattatcatg
     1561 taatttgtga aaactgcggt aagattgttg attttcatta cccagggctt gaagatatag
     1621 aaatggctgc aagcagactg actgattttg agattaatga acatcggttg gaattgtatg
     1681 gactctgtcc ggattgtaaa gagtcagcaa aaaaggcgca aatgtaaaaa cagtttctac
     1741 aagtgttata cctaaaaaaa gctagctgtg taaaaaaaac gctagctttt ttatttttt
//
LOCUS       DACDMM010000262         1796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDMM010000262 DACDMM010000000
VERSION     DACDMM010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.268"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="IWD16_002751"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="IWD16_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9089721.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DACDMM010000263         1788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.269, whole genome shotgun sequence.
ACCESSION   DACDMM010000263 DACDMM010000000
VERSION     DACDMM010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.269"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            540..1670
                     /locus_tag="IWD16_002752"
     CDS             540..1670
                     /locus_tag="IWD16_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9089722.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
BASE COUNT          535 a          287 c          363 g          603 t
ORIGIN      
        1 tatttattcc gaaaagtgaa aaaacatata aaaaaacaca taaaactaaa aaattatcaa
       61 agaatcctgg tgcaactgaa agaatttgca acgggattct ttcaacttat ttgttatttt
      121 tttctaaaaa atattaaaga ttgttaaatt tcactatttc atttcaaatt ccattgccta
      181 atcattagga aaagtctatc atgaactcgt aaatagaata tagtaataac cttgttaggt
      241 gaggctccta tacaaacata ggctgttgcc cagaaacgtc gaaagtcgcc aatgggtaaa
      301 acagaaactg ttggattaag gctttttcca atagctaaga atgttttctt tacgttgtat
      361 agtgctaaaa ctcggacgat gagatgatgc taggtcagaa ttcttgttcg aaaaggtttc
      421 tgaccttttc tttatatttt tgtgactgac ttatttgatg agacaaactc gctcctcacc
      481 tgacaagtgg attgctgtgt gaggaggaga tgcggtgaaa aaagatcgat tggtggattt
      541 tgggtcctta tttgactttg tccatgatcg gtcttttaga agtatacagt gccagttctt
      601 atcgtttatt gcaagccgat gaaaatacaa aaagtctatt attgcgtcaa ctaattttca
      661 tatttttgag ttggagcgtg atcttcttag ctcgttcagt caaactacac tatttacttc
      721 accctaaaat agcaggatac ggtttagcct tatcgatttt ctttttagta ttagtaagaa
      781 tagggatatt cggtgtcact gtcaacggcg cacaacgttg gatctctctg tttggcattc
      841 aattccagcc ttctgaactg gcaaatcttt ttttgatttt ttatttaagc tggttttttc
      901 gtgacggaaa tagtagccca aaagatctaa aaaaaccatt cctgattaca gtaggtataa
      961 cttttctgat tttatttcag ccaaagattg ctggagcatt gatgatcctt tcgattgcgt
     1021 gggtcatatt ttgggcagcg gcggttccat ttaaaaaagg gatctatcta atcgttactt
     1081 tttctgcatt gctgattgga gcagcaggcg gggtattata tttaggaaat aaaggttggc
     1141 ttccacagat gtttaatcat gcttatgaaa ggatcgcgac tttaagagat tcttttatcg
     1201 atagtcatgg tgcgggctat caaatgaccc attcgtttta tgcactatat aatggtggca
     1261 tatttgggcg gggattagga aatagtatca caaaaaaagg gtatcttcca gagacagaaa
     1321 cagattttat tttttcgatt attacagaag agcttgggct aatcggtgca ttgtgtgtgc
     1381 ttttcttatt gttctcatta tgcatgagaa tcttttgttt aagcagtcgc tgtaaaaatc
     1441 agcaagctgg attgtttctt ttaggattcg gaactttgct attcgttcag acgataatga
     1501 acgtgggaag tatcgcgggg ctgatgccaa tgactggtgt gccattgcca tttgttagct
     1561 acggaggaac aagttatctg atcctttctt taggaatcgg aatcacgttg aatatttctt
     1621 caaagataca ggccgaagag ttggcgctct atcgacctga aaaacaataa agagaaactg
     1681 agaaactctc agtttgaatt atcataaagt acaatctttt atttttcaag cgagaggttc
     1741 ttttagaaag aggaattcgc aatgaaagta tctcaaaaaa tcatatta
//
LOCUS       DACDMM010000264         1774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDMM010000264 DACDMM010000000
VERSION     DACDMM010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.270"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            198..794
                     /locus_tag="IWD16_002753"
     CDS             198..794
                     /locus_tag="IWD16_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9089723.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(852..1334)
                     /locus_tag="IWD16_002754"
     CDS             complement(852..1334)
                     /locus_tag="IWD16_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089724.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1440..1637)
                     /locus_tag="IWD16_002755"
     CDS             complement(1440..1637)
                     /locus_tag="IWD16_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089725.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          597 a          360 c          270 g          547 t
ORIGIN      
        1 aaaaattcct aaaacacaac aatgaaagcg ataactaaga aaatccttac ataacgattt
       61 ttcttattaa aattgttttt tgaacttcgc ttattttttg tgagcatatc tgtttactta
      121 ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa aaaaatgcaa aacgcaaatc
      181 aaaggagaaa ttttattatg acaactttta ctgacacttt gaaaaaccgc cgttcaatct
      241 atcatctagg acgtaatgtt tcactttcta atgaagaatt aacaactttg atcaaagaag
      301 caattaaaga aagcccaact gctttcaatg ctcaatcaac acgcgcagtt atccttttcg
      361 gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc acttcgccca ttgactccag
      421 cagaagcttt cccaaataca caaaacaaat tagctggttt caaaaatggt tatggtacag
      481 tattattctt caaagatact gacgtggtaa aaggtctaca agaacaattt gaattatatg
      541 cagacaactt cccagattgg tcagaacaat caaacggaat cgcaacagct aatacttggg
      601 tagcattagt tgataaagga ttgggcgcaa acttgcaaca ttacaaccca gttattgacg
      661 aagcagttgc aaaagaatgg aatatccctt caaactggaa actacgttcg caattagtat
      721 tcggttcacc agaaacaccg gctggggaaa aagaatacat gaacgatgcc gatcgcttcc
      781 gcgtatttgg ataattttat ttgaaataaa aaacagaggt tgtgacaaac atcttgtcgc
      841 aacctctgtt tttattctgg atcaatcata atatcaatct ctgcctcatc gtttaagcgt
      901 tgggacaaaa atggaactaa ttcttctaat gatgcagaaa atttcagcat cggtcctcgc
      961 catccgtttc ggaaaaaatt catttttacg attttagaac cttttggctg atataataca
     1021 attttattga tctcagaaaa aggaacacct tttttgtcaa agctattcaa aacaagtcct
     1081 tcttctgtga ttcctctact gtccaacaaa aaactaataa aaatcattgc tgacaagatc
     1141 ccttttgcca attcatctgc tgtaggatga ctgatgagtg tataccaaat gactagtgac
     1201 actaagaagg gtatcaccaa gtagacccat cgatttcgca cagtccgata aataatttct
     1261 tttcgaacca gccaaagaaa taaaaacatc acaacaacga ataaaaacaa tagaaccgta
     1321 aagaatgtta ccatgacaac tcgcctcgct ttttcttctc cagaaaatag gaaaagaata
     1381 ctgatttacc ttctcaaaga aattggactt gcagcacgcc gcacgtccaa ttgttttctt
     1441 tattcaaatg tatcatattt tgcaacaacc atgcttgctt tttcatctaa taattcttgg
     1501 tcctctgcag cacattgggc aactttgacc acggcgcagt ttgtcatttt tgatacaact
     1561 tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg catattgttc tcctaaatta
     1621 atcatttctt ccgtcatcaa aatcctccta aactgctgga acttttctcc acattaccac
     1681 tgctatttct ttggagattt aactcattga tttttattta cagttaaaaa tcaaccttac
     1741 aggcattata gcgtactgtt ttcgttttca caat
//
LOCUS       DACDMM010000265         1731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDMM010000265 DACDMM010000000
VERSION     DACDMM010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.271"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            275..934
                     /locus_tag="IWD16_002756"
     CDS             275..934
                     /locus_tag="IWD16_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9089726.1"
                     /translation="MKIVAIVPMKLNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQI
                     DKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTT
                     APFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLY
                     EETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQG
                     S"
     gene            953..>1731
                     /locus_tag="IWD16_002757"
     CDS             953..>1731
                     /locus_tag="IWD16_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAP9089727.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYI"
BASE COUNT          669 a          197 c          326 g          539 t
ORIGIN      
        1 ttgtggaatc ttggggatta ttgtttgttc aggcaatcat agaaatatat tttaaaaatt
       61 atgtaataga aattataatt atgattattt taattattat aaatatcagt tatattaagc
      121 agttgtgtta tagaggaaga aacattattt caatgattaa gagtcgagag cacaaacggt
      181 agacgatgtt atttttataa aaagatataa tttattaatg gtcgataatt atgaaaggaa
      241 aaatattctt aaaaaaaaga gtataggatg tcgtatgaaa atagtcgcta tagtaccaat
      301 gaagttaaat aacaaaagat tgccacaaaa aaacacgaaa gcttttacca atggaaaacc
      361 attgtgttat tatatacttt caacactttt aaaagtaaaa cagattgata aagtgtatgt
      421 ctattgtagt aacccagata ttaaagaatt tataccagaa ggtgtaacat ttttaaagag
      481 atctagttcc ttagatcagg atacaacgag tattacacag gtattagccg gtttcacaaa
      541 agaagtagag gctgatgtat atgtaatgac acacacaaca gcaccattta ttagcgaaaa
      601 atctattgaa aaaggactag atgccgtgct gacaggagaa tatgattctg catttgctgc
      661 aaaaaaattg caagatttct tatggaaaga cggtgaacca tttaattatg aattggacaa
      721 tattcctaga acacaggatt tgccgccttt atacgaagaa actagtggat tttatattta
      781 taatcgcaat gtgatgaccg agttgcatag aagaattggg aaaaaacctt gcatagttga
      841 agtaggtgag attgagtcag ttgatattga tgaagccgag gattttatga ttgctgatgc
      901 gatttataat catcttattt tacaaggtag ttaagacagg gggaaggtta ggatgaacaa
      961 tgtacagatt ttagattgca cacttagaga tgggggctat tgtaacaatt gggaatttgg
     1021 atttgataat acaaaaaaaa ttatttctgg acttatagaa tcaggaattg agattataga
     1081 atgtgggttt ctcacaaata aaacagtata caataaaaat gttacaaaat atacgacctt
     1141 agaagaatta gcacagataa ttcctgaaaa aagagaagga aaattatttg tagcaatgat
     1201 gaactatggc gaatataata tagaggatct tccagattat gatggttcat ctattgatgg
     1261 aattaggatt gcgtttcata aaaaaaattg caatgaagcg ttgaaattat gtcgtgagat
     1321 aaagaaaaaa ggctacttag tttttgttca agctatggta tctctcaatt atacagatga
     1381 agagttttta gcagtaataa aaaatgtgaa tcagtttaaa ccatacgctt tttatattgt
     1441 tgatagtttt ggaatgatga aaggaaaaga tttaactcgt cttttttaca tgattgagca
     1501 taatctagaa aaagatatac agataggttt tcactcacat aataacatgc agcttgcata
     1561 ttctaatgca caaaaactta taaccgtaca aacaaatcga agcctgatta ttgattcttc
     1621 ggtgtacgga atgggaagag gagcaggtaa tcttaacaca gaactatttg tagaatattt
     1681 gaatgataat gcaggaaaaa aatatcaatt aaaaccatta ttatatataa t
//
LOCUS       DACDMM010000266         1706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDMM010000266 DACDMM010000000
VERSION     DACDMM010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.272"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..742
                     /locus_tag="IWD16_002758"
     CDS             <1..742
                     /locus_tag="IWD16_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP9089728.1"
                     /translation="NLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNL
                     ENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQE
                     NELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIA
                     NYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSH
                     FSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            759..1193
                     /locus_tag="IWD16_002759"
     CDS             759..1193
                     /locus_tag="IWD16_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089729.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
BASE COUNT          643 a          197 c          288 g          578 t
ORIGIN      
        1 aaatctaagt aaaactatcg agcaaatcaa aatagataat ctgttatcac aaaattacga
       61 tccagaaata tatctgtttc tttatgaaaa taaaatactg gaatatgtta aaaatggaga
      121 tattcgaaat ttagaaaaca tggtttttaa tctgagtaat ggaataattc caagtgttag
      181 tggagatacg attcggtctg aaaaaaatta ttctatcatt gtttttgaaa agttagcaca
      241 aacgtctata acattaggaa tggatataat agaagcgtat caaagtagag atgctttgat
      301 acaagaaaac gaattagctg tatcgttacc tgaggtctta aaagtgaggg attctgggat
      361 tgtttattat actaaggaaa tcggaaaaac taagatagag catctttctc ccttaatttc
      421 ttctgtcgtt caattcatcg gcttaaatat ctataaaaga attactgtaa aggaaatagc
      481 taattatttt tctgtaagtg aaactaaatt aagagaaagc tttaagaatg aaatgcatat
      541 tacgatttat aattatatct ctaagcgaaa aatttctacg gctaaaataa tgttgaaatc
      601 aaaccatact atcagtgaag tttctttagg tttaggattt tcagattctt cacacttttc
      661 gagagtattc aaaaagtacg caggtgtatc tcctaagcaa tatcaattag gtttagtgga
      721 caatttcaac aacattagct gagaggtttt attttattat gagactatat catttacgta
      781 gtttggagga gttgaggaaa ttggatttta acaaaaaatt tttttgggaa atttctgatt
      841 tgagttgttt cgtagaatct gagcgtctaa gtaatggtaa taatagtgtg ttaaatgaat
      901 atgacaaagt agtaattaat aaagtaaaaa gtttatgtga agaagctaga gaatctatca
      961 gtcatattaa tggtagcaat tttaaacaaa cattatttaa tctgattaag ctagatgcta
     1021 aaatatcaag ttatttattc tttttgatac atgatgaatt tatggattat caaaaattgg
     1081 atcagttaat tgaaagagaa agctgggaag attattacgt gggtctaact ttttcagaaa
     1141 agttaaacaa ctacaagtta atagattata agtatttgag tgaaagcaat taattattca
     1201 tgtagagtac tagacacttt aaatatggac aattcaatta taaagaaaaa gaatcaataa
     1261 tttaatgagg ctgtgacaaa tgtcattttt tgtttcgcta aaaacgagtg aaaaaatgtt
     1321 gataactcca atcattcgtt taagattcta ggaatcattc agaaatgctg tgaaaccgat
     1381 actttataat tataaagaag gtgatttcaa attccgaaga aggaatttga agtcaccttt
     1441 ttacttatgt cacagtctca ttcagtaatg cgggataagg ctccttattc cgttatttga
     1501 atactctgaa gtttaatcta agaaagggta atattttgaa aacatgcttc atcgcataga
     1561 aatgaactta atttcagaaa gaatacatac atatcttttt atatgttttc agctgctgaa
     1621 tggttaaaaa gcacaaatat ccgaaaaaaa catgaagaaa tgtacttttt ttgttgtaat
     1681 gttcatgaaa ttctctaagt aaatta
//
LOCUS       DACDMM010000267         1689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.273, whole genome shotgun sequence.
ACCESSION   DACDMM010000267 DACDMM010000000
VERSION     DACDMM010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.273"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            949..1422
                     /locus_tag="IWD16_002760"
     CDS             949..1422
                     /locus_tag="IWD16_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP9089730.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IWD16_002761"
     CDS             1431..1658
                     /locus_tag="IWD16_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9089731.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          569 a          267 c          249 g          604 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaat
//
LOCUS       DACDMM010000268         1658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.274, whole genome shotgun sequence.
ACCESSION   DACDMM010000268 DACDMM010000000
VERSION     DACDMM010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.274"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /locus_tag="IWD16_002762"
     CDS             <1..672
                     /locus_tag="IWD16_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP9089732.1"
                     /translation="SLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLELL
                     GTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMIF
                     AAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQV
                     LFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKYY
                     PGKGQ"
     gene            674..1618
                     /locus_tag="IWD16_002763"
     CDS             674..1618
                     /locus_tag="IWD16_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP9089733.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
BASE COUNT          449 a          277 c          413 g          519 t
ORIGIN      
        1 agccttttag gcgtcttgat tgttattctc gggtttgcat ttaaattaga ctcgatattg
       61 atcgtaatgg ttgctttagt tgttacagct ttgactggcg gtcttggcat tcaaggaatg
      121 ttagaacttt taggtacaag ttttgtaaac aatcgcggga tggcgatttt tatcatcatt
      181 atgttggcaa caggaacgct tgaaagaaat ggtctgaaag aatcagcggc ttccttgatc
      241 aagcgattca agaaagtatc tgctggtatg attgtagata tttatgggat ctttcgaatg
      301 atctttgcgg cttttaacgt gagttttggt ggagttgctg gatttgttcg tccaatcatt
      361 ttaccgatgg cattggggac catcgaatca aaagggttag aagtccatcc taagcatgaa
      421 gaagaactaa aaggtatggc ttcagctatg gaaaatgtct gctggttctt tggccaagta
      481 ctattcatcg gaggagcagg ggcgttgcta gtccaatcaa cactgaaaga tctaggatat
      541 gacgtaacat tgggacagct tgctttagtg gaagttccag tggctatcac ggctttagtt
      601 gtggcaagtg tctacttcta tcttaaagaa aagaaattag cgaagaaata ttatccagga
      661 aaggggcaat aagatggact tttttacaaa tcctgatatt ttattaggcg ataaattact
      721 ggagatcctt tatatcgtga tgggactcgt attgatttac actggagtaa gaaatttaac
      781 agataaaacc aatcctcatc gttatgggtc agcctttttc tggacggtct taggggtagt
      841 tattgcagga ggaagatggc ttccggcaat cgtgaacggt ggcttgatct ttgcgatgac
      901 cataccagca attgctaaga aagtcagcaa aggagaaagc cggcttccat caaaagaata
      961 tatggaaaaa atgtcagaca aactaggaat gaaaattttt attccagctt tgagtatcgg
     1021 tgtctttgcg atcttgtttg ctctatttac caatttaggt gctttagtcg gcgtgggcgt
     1081 cggtgttttt gcagccatgc taatcttgat gttcttttct cgagataatc gaccaagtac
     1141 atttttagat gatgcagcag atatgctggg cacagtaggg ccgttaagca tgttgccaat
     1201 gcttttagct tctctaggtg cggtatttac aagcgctgga gttgggacag tgatttccaa
     1261 tgcagtaggt acgatcgttc cagaaggaaa tgtgaacatc ggcatcatca tttacgctct
     1321 tggcatggtg atatttacgg taatcatggg gaatgcattt gcggccatca ccgtgatgac
     1381 ggttgggatc ggtgcaccat tcgtatttag tcatggagcg aatccagcgt taataggaat
     1441 ggttgcattg acttgcggtt tttgtgggac cctgctgaca ccgatggctg ctaacttcaa
     1501 tatcgtgcct gtagcgatgc tggagatgaa agacaaatac ggagtgatca aaaaccagtt
     1561 gtttattgcg ttatttatgc tagttttcca aattgcttat atgattctat ttaaataata
     1621 gaagagaggg gttgtgacag aataattgtc gcaacctc
//
LOCUS       DACDMM010000269         1648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.275, whole genome shotgun sequence.
ACCESSION   DACDMM010000269 DACDMM010000000
VERSION     DACDMM010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.275"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            138..1604
                     /locus_tag="IWD16_002764"
     CDS             138..1604
                     /locus_tag="IWD16_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP9089734.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          462 a          296 c          337 g          553 t
ORIGIN      
        1 tttttatgat attgccatta aaaattactt gcttttatag aaaattagtt tatatttctt
       61 aaataagttt tttagttaaa tttttcggta aagatgtata atattttctg aaaatttaaa
      121 gaattggggt atagattatg gaaaaacagc aagacaaggg cttcttcggg cagccgaagg
      181 gattgtcaac tttgttcttc actgagatgt gggaacgatt tagttattac ggtatgcgtg
      241 cattacttat ctattatatg tacgatacag ttgccaacgg gggacttgga ttaccacgag
      301 caacagcatt agctatcatg tcgatctacg gatctttgat ctacatgtct agtatcgtcg
      361 gcggatgggt atctgaccga gtgttaggag caaaacgcac tgtattcttc ggtggagttt
      421 tcattatgct tggccacatc gtcttggctt taccatttgg tgtaagtaca ttgtttgttt
      481 cgatggcatt gattattatc ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg
      541 gacatcttta ttcaaagact gacttgcgaa gagatgcagg attttcgatt ttttacatgg
      601 gtgtcaactt aggggcatta ttagcaccac taatcgttgg tacaatcggt caagaatata
      661 actatcattt agggttctct ttagctgcta ttgggatgtt ctttggatta ttacaataca
      721 tcatccaagg acgcaagaca ttagacggag tgggtacaac accgccgaat cctttgacaa
      781 ctgaagaacg taaaggattc atccgtaact tgatcatcgc acttgttgtt atcgctgcag
      841 tattcggcgg ggcacaacta actggtcatc tgacaattga cttttttgtc aatacaatca
      901 gtgtattggg gattatctta ccgttgtatt actttatcaa aatgctgact tcaaaagatg
      961 taactgctga agaaaaacca agagtatggg cttatatccc attattcctt gcagcaatca
     1021 ttttctggtc attacaagaa caaggttcat ctatcttagc tttatttgct agtgaacgta
     1081 cacaatctac gctatttggt gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa
     1141 ttttcatctt tattctgaca ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc
     1201 aaccatctac agtagtgaaa ttctctcttg gacttttatt tgcaggatta tcgtacttgc
     1261 tattgatgtt gccgggtatg ctttatggta caagtggaaa agtaagtcca ttgtggttgt
     1321 tgggaagttt cttcatcgtg gttattgctg aattgtgtct ttcaccagtt ggtttgtctg
     1381 tgacaacaaa attagcacca cgagcatttg aatcacaaac aatcgctatt tggttcttag
     1441 ctgatgcttc ttcacaagca atcaatgcac aaattgcacg cttctacaca ccgggaacag
     1501 aatctgctta cttcggtatt atcggtgcag tagcaatcgt cggtggtatc ttgctattta
     1561 ctgtgaaaga accaatcaaa aaactaatgg gctctattca ttaatcaatg aacataaaaa
     1621 caggaattgc ttatttgcag cagttcct
//
LOCUS       DACDMM010000270         1626 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.276, whole genome shotgun sequence.
ACCESSION   DACDMM010000270 DACDMM010000000
VERSION     DACDMM010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.276"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(61..279)
                     /locus_tag="IWD16_002765"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="IWD16_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="IWD16_002766"
     CDS             complement(545..739)
                     /locus_tag="IWD16_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089735.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          574 a          242 c          217 g          593 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagta
//
LOCUS       DACDMM010000271         1597 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.277, whole genome shotgun sequence.
ACCESSION   DACDMM010000271 DACDMM010000000
VERSION     DACDMM010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.277"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..513
                     /locus_tag="IWD16_002767"
     CDS             <1..513
                     /locus_tag="IWD16_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9089736.1"
                     /translation="KNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFEGG
                     KGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSARTSK
                     EKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVKMP
                     GLMAALKRVY"
     gene            532..>1597
                     /locus_tag="IWD16_002768"
     CDS             532..>1597
                     /locus_tag="IWD16_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9089737.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMES"
BASE COUNT          605 a          201 c          304 g          487 t
ORIGIN      
        1 aaaaataacc aaattctatt taaaacttat aatagttgtg aggatcgaat atttaactta
       61 cattattatg aatactgtca tcaagtgatc atgaacccaa aagtagaata tatttatgaa
      121 tttgaaggtg ggaaggggat cactaatcaa taccgaccta acaagttcga aacatttaaa
      181 gaattctatt cattagcaaa tactgtaaca gacgatatta ataaagacgg tatggcggcg
      241 cttcttttaa aagggacaac ttcagttatt ttttctattt atagtacctc tgcaagaact
      301 tcaaaagaga agaaagacga agcaaagcag atattgagtg atccagtgat ccgagaagcc
      361 aagcaaattg ctaagacgga ttcttcagca aaaaaaataa caaagttgct ttacaatatg
      421 ccagctccac tatttttatc tgctgtaaaa gttggaagct tcgttgaagt gaaaatgcca
      481 gggttaatgg ctgctttgaa gcgagtatat tagttaatgg aggaaaatat aatgaaaaga
      541 attttgcatt ttcagggacg aatgggttta ggtggagcag agtctttcat gatgaattta
      601 tatcgtaaaa ttgatcgtac aaactatcaa tttgattttt tgatttatga agattatgca
      661 gatgttcaag actatcattc agagattgaa agattaggag gccgtatttt tgttgtacct
      721 aatcctaaga aaaatatttt taaatattta atagaagtga ataagttact aaaaaaagaa
      781 tctttttcta tagttcacaa tcaggtttat tttggcgggg gaattaattt atggttagcc
      841 aaaaagaatg gaatcagaca gagaattgct catagccatg caactgaaga tggaaaatct
      901 caaaatattg taatgaatgt attgcgtaaa tttttaacca aattgttgtt acaaaatgca
      961 acagactacc ttgccgttag tcaagaagct ggtgaaagtc tgtttcaaaa ccatcccttt
     1021 gagatagttc ataatggtat tgatttagag ctttatagta aaaattcaga agctaaggtt
     1081 aataagagaa aagaattgga tatctcaatg agtacttttg ttgtgggaaa cattggacga
     1141 ttagagaaac aaaaaaatca atcttatttg ttagagattt ttttagaaat actaaaaatg
     1201 aattctgatt cttacttgat tttagtaggt gaagggtcac ttagaaataa actgaaagcc
     1261 aaagcagtag aattggatat agagaaaaga gtaaaatttt taggagaaag aaacgatatt
     1321 cccgagttat tatctgtgat ggatgttttt gtaatgcctt ctctttatga aggtctgcca
     1381 atggttggat tagaagcaca agcagcaaaa ttaaaattag tcttagcaga tactatttcc
     1441 aaagatacaa agttaacaaa taatgtggat tttgtatcat tagatgaaac accacaagaa
     1501 tgggcaaaaa aagtaataaa agagccgtat gctaataaaa taacacaaga actaaagagt
     1561 tatgatgttt cgtacactac tgaacaaatg gagagta
//
LOCUS       DACDMM010000272         1579 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.278, whole genome shotgun sequence.
ACCESSION   DACDMM010000272 DACDMM010000000
VERSION     DACDMM010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.278"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            17..439
                     /locus_tag="IWD16_002769"
     CDS             17..439
                     /locus_tag="IWD16_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089738.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            449..652
                     /locus_tag="IWD16_002770"
     CDS             449..652
                     /locus_tag="IWD16_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089739.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            863..1447
                     /locus_tag="IWD16_002771"
     CDS             863..1447
                     /locus_tag="IWD16_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9089740.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          557 a          225 c          293 g          504 t
ORIGIN      
        1 ttcgaggtga tagacaatga aaaataagtt tattttatct ttaggtattt taactattgg
       61 aattagtgta actggaacta caattcctac atttgctgat agtacaaatg aaccaactac
      121 tgtagtagat aataaccaag aattaactcc tttagatagg gacggaatag ccattggtaa
      181 cccaactaac gacttaactg ttactgaaaa ttatttaata gtttctccat tacttgctct
      241 agcagcttct aaaacgatac gagtgacagg aacgatgatt tttgataaag ttggttggcc
      301 ttctagacct cctcaatcac ggaattggaa agaaaaaaga aatggttatt ggtactatgg
      361 aaaattgtat ttacaaagtt ataaatcagc tggaaacaat tggattgcga cttattcagg
      421 aaccttaact agaaagtagg gattttttat gccagaattt gtgaacacac caaattttta
      481 tcaagatagc gatattctaa gtaaaattaa tttaccgaaa gaagaatcag ctttacaagt
      541 tcagcgaact ttacaggaga cacaaaaaat tcaaaaatca atttctaatc tagaagattc
      601 tgaagaaaaa agaaaaaaaa gagaccaaaa aacatatcaa aaagaaaaat aatgatttta
      661 aaaaaagagt gtctagtcga cattcttttt ttctaattga aagtgttcat aaaatcaccc
      721 atttctggac actttctttt tttgctttat ttttcaaaaa aacaattcat ttcatgttca
      781 taaacccata acaatatcta tgttcagtta aactatatgg ataaagttat gttacaataa
      841 aattaatgaa ggagtgattt ttttgaaaat aggatacgcc cgggtatcta ctggtttaca
      901 aaatttggat ttacaagaag acagcctaaa taaatttggt tgtgaaaaaa tttttactga
      961 tcatatgagt ggggcaaaaa gtaatcgacc aggattagaa atggccattg attttgttcg
     1021 ctctggcgat acacttgtcg tttggcgctt agatcgttta ggtagaaata tggaagattt
     1081 gatttcaatc gtcaatcgac tgaatgagcg tggtgtgagt tttcatagta ttcaagaaaa
     1141 tattacgatg gacaaatcaa gttctacagg acagctcatg tttcatttgt ttgcggcatt
     1201 tgcagaattt gagcgtaatt tgatcctgga acgatccgca gcaggacgag cagctgctcg
     1261 tgcaagagga cggtttggtg gtcgtcctga aaaattgtct gaacaagatt tggaattact
     1321 gaagacttta gtggatagcg gtacacctat taagactatt gcggaacggt ggaacgtctc
     1381 aagaaccacc atttatcgtt atttggcaaa aatggaggag aaaaatggaa agacaaattc
     1441 tcattagttt ttgtcattta atgctgaaaa tttttgccat ttatctagaa aagtgacaat
     1501 agtatgagaa ttttttgtaa gtgtctactg ataacttatt ctcaaatgtt gtaaggattt
     1561 tattcatttg ttaatatta
//
LOCUS       DACDMM010000273         1567 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.279, whole genome shotgun sequence.
ACCESSION   DACDMM010000273 DACDMM010000000
VERSION     DACDMM010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.279"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(980..1498)
                     /locus_tag="IWD16_002772"
     CDS             complement(980..1498)
                     /locus_tag="IWD16_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP9089741.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          488 a          300 c          212 g          567 t
ORIGIN      
        1 actataccct atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact
       61 actagtttgt aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca
      121 cctacaactt ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat
      181 agtaaagttt gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct
      241 tctgaaaact tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa
      301 actaagcaat aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt
      361 attctgataa atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa
      421 gatctcttca gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa
      481 taaactgctc aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca
      541 aattataaac cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt
      601 cagaagatat aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt
      661 taaagggaat tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt
      721 tcccctgcta gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc
      781 gaagtgagtc atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc
      841 tctacggcat tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa
      901 gaacttatgt agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg
      961 cttagcagta aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa
     1021 aagtcccaaa tggagcaact gtatttacat gaataaattt atatacttcc catacagccg
     1081 ttttggttgc ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca
     1141 ttgaatcaat catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt
     1201 tcaatggtag atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc
     1261 atttaaattg atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt
     1321 ttagaattaa attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca
     1381 cttcctcaac tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt
     1441 tagtataagt cctatttatt tcttttttta attcttcttt atttttatat gttctcatta
     1501 tattacctcc ataaattcct aattgtctcc tttaattcat ctataatttc atccacgata
     1561 ttttaat
//
LOCUS       DACDMM010000274         1539 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.281, whole genome shotgun sequence.
ACCESSION   DACDMM010000274 DACDMM010000000
VERSION     DACDMM010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.281"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..334)
                     /locus_tag="IWD16_002773"
     CDS             complement(<1..334)
                     /locus_tag="IWD16_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9089742.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRR"
     gene            complement(630..1502)
                     /locus_tag="IWD16_002774"
     CDS             complement(630..1502)
                     /locus_tag="IWD16_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9089743.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          413 a          353 c          304 g          469 t
ORIGIN      
        1 accggcgata gagggtactt actgaacaag agaaggttcg ctctgcacgg ccaataatga
       61 cgtctggggt ccaaccttca gttgatcttt ttgcaatgta ttctttttcc tcagcaggaa
      121 aaatgatttc ggttctccca caacgtcgct tattttcttt gtattgttca aagcattcaa
      181 gtgctgttcc accacatttg agaaagttat agacattgta aattgtttgg cgtccacgtt
      241 taagctgctt cgcaacgata gcaaccggag tttcttgatg aaaatatgct tctatcatta
      301 caagctcgtt tggtgtaaga tgggtataag tcatttatgt tcactctcct tgtatgcttt
      361 agcgggtatt acgatttgag tgtaacataa atggcttttt tatttgtctc gcttaattat
      421 acaaacggcg tgtaaaaaac tattgcacaa tacaagtggc ctacggctat cgaaatttcc
      481 ataactttcg agcaagaatt tatctcattc aaggcttgat cttccaagta aaacaaaaac
      541 cagttaagca ctctgcctaa ctggttatga ctaaattttc agttgtatct ttctctacca
      601 acactaaatg tcgaagagcc ttttttcgtt tatgaatgta agaccgcttc tcgagccaat
      661 gccaaacaac cgagcgttcc agcgttgtct tctaatgctg gggtcacgat atatttgctt
      721 aactcaggtg tttcgacata cccattgacc aattttgcaa atgcgtcgtg gacgttttgc
      781 agcatatggc gttgtttcat cacaccaccg ccaaaaatga tcacatctgg agaaaacatc
      841 aaggttgtat tgtaggcaca ttgagcaata taggaggctt cgatttccca ataaggatca
      901 tcttctgcaa cctcttgtcc ttttttccct aagcgttttt cgatggcggg tccagcggcc
      961 atcccttcta aacagttgcc atgaaatgga cagctgccag caaagtcatc ttttggatga
     1021 gggacgacga gcatatgccc catttctgga tgactaaacc cttcgataaa gcgcccttct
     1081 tgtaaggcac cgccgccaac acccgtaccg atcgtatagt aaacgacact gctcaatcct
     1141 ttgccactgc cgaagacata ctcaccatag gcagccgcat tgacatctgt tgtccaagaa
     1201 atcgggatcg gaaattcttt tttcatggta ccgatgaaat caaaattttg ccatgccaat
     1261 ttcggggtcg aggtaatgta cccataagtg gcggaatcgc gatggatatc gataggccca
     1321 aatgagccaa tcccaatccc tgctaaatct gcttggtatt gtttgaagaa gtcaatgact
     1381 aaaggcattg tttcctcagg tgtcgttgtc ggaaagctca ctctttcctt gatcgtcatt
     1441 tcttcatcgc caatggcaca cacaaatttt gtgccgcctg cttcaattgc accgtatagc
     1501 atagacattt ctccctttat tttagaatga aatcactaa
//
LOCUS       DACDMM010000275         1537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.282, whole genome shotgun sequence.
ACCESSION   DACDMM010000275 DACDMM010000000
VERSION     DACDMM010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.282"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..974
                     /locus_tag="IWD16_002775"
     CDS             <1..974
                     /locus_tag="IWD16_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP9089744.1"
                     /translation="IDVTYKCNFRCKHCYNASGGDNCFMDNELTDDELLELTKDICNL
                     KPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTNGSLVTQDVAKELFKAGINR
                     FQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEEVDVAFCPTSFNYNQLREVY
                     QLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDINYIKRNEDYTKIDWGD
                     PIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCHSLSKYWRAGLYKVFS
                     LDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILDEYKHDILI"
     gene            1030..1248
                     /locus_tag="IWD16_002776"
     CDS             1030..1248
                     /locus_tag="IWD16_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089745.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
BASE COUNT          574 a          182 c          293 g          488 t
ORIGIN      
        1 ctattgatgt tacatataaa tgtaatttta gatgtaaaca ttgttacaat gcgagtggtg
       61 gagacaattg ttttatggat aatgagttga ctgatgatga actgttagaa cttacaaaag
      121 atatatgtaa tttaaagcca tataatattt gtttctgtgg aggagaaact ttactgagaa
      181 aaaaaatagt tttatcttgt gccactttat taaaagaggc aggtattcct aatattgcta
      241 ttgttactaa tggatcatta gtaactcagg atgttgcaaa agaattattc aaagcaggta
      301 ttaatcgatt tcaagtaagt ttagatggaa aaaatgctag tagccatgaa gcacttagat
      361 tatatccaaa cagttttgaa aaggcaatta atgctatcaa attatttaga gaattgtccg
      421 aagaagtgga tgttgctttt tgtcctacta gttttaatta caatcaatta cgagaagtat
      481 atcaactagt aaatactctt aaagtgaagt ctttaagaat tcaaccattt atgcttagtg
      541 gtaggggaat gcaaaatagg gaaatagtgc caactgtttc tcaatataga aaactagttt
      601 ctgatattaa ctatattaaa agaaatgaag attataccaa gatagattgg ggagatccaa
      661 ttgatcatat catccgacac agaactttat tgaaagattt ttcgaatttt gtttctatta
      721 ggtcagacgg aggattaatg gcatcacctt acttgccaat tcaagttgga aatattaaat
      781 gtcacagtct ttctaaatat tggcgagcag gactttataa agttttttct ttagatttag
      841 ttcaagatat agctactcaa gtttcttcaa tacaagatat gagtaaagaa ttaaaaaaag
      901 gacatattaa atggcttaat gaagatatag aaattgatat tctcgatgaa tacaaacatg
      961 atattttaat ttaggtttat cattggaaag tttagataaa caaagtactt ttagaaagga
     1021 gggaaaaaaa tgatttctga acctcaaatg aataatatgt atgaaagccc aactataatg
     1081 ggagctggtg aagctgcgcc aagagcttgt tcagcaatta tggtgtgtgt gttattagca
     1141 tctgcaggag taggtatcca agcaggagcg gtgtatactt atgctgtagt tgctacagta
     1201 gctgtagctg cagccgcagt ttggaaaaca gtaggattag ttggttagga attaatagga
     1261 gactaactgt ctcctattga ttccaatgat taagctaagg aggaatttta agaaaataat
     1321 gaaatacaat aatttaaaac cgaaattact taatgagaac atagaatata taagagaaga
     1381 aaattatgga tatttaacca tagttcccaa aaatggaact gaattgaaag ataacgtttt
     1441 caatccaaca gtaagatata tattggctaa ctgtactgga aaaaacagtg taaaaaaaat
     1501 agcgaatggt ttaaaaaaga agtttcctag tttagaa
//
LOCUS       DACDMM010000276         1526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.283, whole genome shotgun sequence.
ACCESSION   DACDMM010000276 DACDMM010000000
VERSION     DACDMM010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.283"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            297..1247
                     /locus_tag="IWD16_002777"
     CDS             297..1247
                     /locus_tag="IWD16_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9089746.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
BASE COUNT          564 a          214 c          249 g          499 t
ORIGIN      
        1 aaagctatga tttcggaaaa taagaaagac gtagtagtta tcaaaaagat tagagagtat
       61 acaaatttag gtgttttaca agccaaaaaa gtatatgaaa aaaaaaaaaa gggtctataa
      121 ttacaaatta gataatacat tagaagctgg agacaagatt gtctagataa tgtgtgagat
      181 aaaatttttg gcaggttatc atacataaga caaaatgttt ttaaaactga taacgtattt
      241 attccacaac aattacgcat ttatttctga gaaaaatata aagcaggagg attacaatgc
      301 tgaaaactta ttccttaaca aaagatacat tattagtttc gtcagaatca aatatcaaaa
      361 attcttctgt tgtttatgtt tatggtgacg aacgagaata tatcgaatct tctgaacata
      421 aatatgactt taaaataggc aatatactaa cgtttgatga tcgagttgca tatgacacga
      481 tgatagataa gaacgatgaa aaaagtttgc tcgtaagttt tttgtttccc gaggtacatg
      541 aaggtgggca tctagatacc ctgacgacat ctgttgtttt cttggtattt aaaaataagt
      601 tgataatttt tacaaaacaa aaattaaaat tcattcccga actcttatcc aatatggttt
      661 tacgtgatgt caatcatttc gtggaggaag tacttttaaa gatcatgcaa aaagatttct
      721 atatgatgct taacgacctt cgtggagtca aggaaaaaat tgatgattta gactcagaaa
      781 tatcaacgtc cggttcactc aaaccaacgt ttggtgattt attgactctt caaaaataca
      841 tgatagcttt gtcaacaact tataaagcta accataaagc gcttgatttc attaaaaaaa
      901 atttcacaac tattgacgat attgatttta gtactaaaaa aataattgat gatttatacg
      961 atacaagtga tacaatggat agaatcattt taagttacag ccaatatttg gacaaattgg
     1021 aaaatatgat aaacaatatg atttcgtatc agctaaacat gattatgaaa accctaaccg
     1081 agatatcaat tgttttgact attccagcaa ttatttttgg cttttgggga atcaatgtcc
     1141 atgtcccgtt tgaaaagtct tcttatggtg ttttagccgt attgattatc tctgctatac
     1201 taagtctaat ttgctggctt ttgatgcgac gcaaaacata tttgtaaagt atttgtgata
     1261 ccctaatatg atttaaagca gaatttcttt gttgactttt taaaaggctg attcctttca
     1321 ataatttaat agcttcagcg gctgctcaga agtcaataaa ttggactatt tttattcagt
     1381 tacttaggca acatttgaga tacgattgcg attataaaaa ttgatatacc aaccaatggc
     1441 tgcttggaag actgacatat tagcctcgat atcatcgaat aactaaatac agaaaaatac
     1501 aataaatatt atcaaactag ggacta
//
LOCUS       DACDMM010000277         1511 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.284, whole genome shotgun sequence.
ACCESSION   DACDMM010000277 DACDMM010000000
VERSION     DACDMM010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.284"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            287..937
                     /locus_tag="IWD16_002778"
     CDS             287..937
                     /locus_tag="IWD16_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089747.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            1124..>1511
                     /locus_tag="IWD16_002779"
     CDS             1124..>1511
                     /locus_tag="IWD16_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP9089748.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLC"
BASE COUNT          483 a          185 c          314 g          529 t
ORIGIN      
        1 tggagattaa ttaaaaactg gaaggaactg ctcgatcaac cttgagaact gtttttctta
       61 cttctggtca agattctcgt ggttggtgga taaggatggt tgataagttc attggattag
      121 ctagtcgaaa acaaccaggt gtaattaatg tagaagtttt aacaagggga tggagtgatt
      181 tattcagtga taatcataag ttcataaata gtaattggga tgtatggttg gtatacgtta
      241 tacctgaact tgaactatca agatttggtt tagaatttga gaatcgatgt ttataaaggg
      301 agggcaaaaa atgtgctttt ttacaacttt tactatcatt atgattgcag tactttttat
      361 ttttctagat atagcaaagc gaaatacagc atttttatta tatcgggttt tacttcgagc
      421 aggtctcata acatttatat cgatcgttgg attttttcta ttcacagtta ttgtatttat
      481 ttggagaact acagcaccac cattgccaga gattacatat ggagaatttc cgtttagtct
      541 tgaatatgaa atagatgagg aaagaatttt gattgaagat acggtgattg cagaatttat
      601 aggaagtttt agagggaatg tgacaactca acctagacga agttggagta taaaactttt
      661 aaataatagt ggagagttga aagcatttca ttatgaattc ttaataatag aaagtgaaga
      721 tgtaacaatc actttaagta gtggctctgc tggatatttt atgaatgatg gtggaagcaa
      781 gccacagctc acagtccgtg atcgtattgc aggttttatg catttttctc actatgactt
      841 agaagaagct cgtaaaatac ttgcagaata tgggatcaca ttaatcaatt gggaatatgc
      901 gccaccgata agaaattttt tcaatgatta ttattagtct tataaaaaac agaatgtaaa
      961 atttatgtgc gcgagtattt agttttccat tttttgtttg atagatatat agcattgttg
     1021 aagcatggga tagtggtgcc aattttatca atgaggttgg tcaggcagtt aacataattg
     1081 gtgatgctat aaatccattt aattggaatt ggaggaatta ggtgtggaaa cagtgagatt
     1141 atggatattc atgaaaaatt ttcgttcggc tttgagatat gttattctgg aatcaaaatc
     1201 aggttattat ctgattgaca cagatcacta tactttttgg ggaggctacc tttttccgat
     1261 ttttaattgg ataatccccc ataaggttat gaaaattaac ttgcctgcag aagaaataaa
     1321 tagattaata ctcaacaaag atttacaaag gaaaagagag aaagaacatt ctaatgttgc
     1381 ttggataggt ccagcatttg cagtagtgac ttcttcaatt gtcggtccta tgctagagag
     1441 tatttatagt gatttttcta ttggaatacg ttatttattt gttactttaa gtatagctgt
     1501 tcttttgtgc a
//
LOCUS       DACDMM010000278         1475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.285, whole genome shotgun sequence.
ACCESSION   DACDMM010000278 DACDMM010000000
VERSION     DACDMM010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.285"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IWD16_002780"
     CDS             <1..848
                     /locus_tag="IWD16_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9089749.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
     gene            1362..>1475
                     /locus_tag="IWD16_002781"
     CDS             1362..>1475
                     /locus_tag="IWD16_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9089750.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDR"
BASE COUNT          485 a          260 c          289 g          441 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttgg ttctgttgca aagttttcca aaaaatctat
     1321 tttagtgtaa aattgagaaa aaagacagag aggacagagt aatgaatcat tttaaaggca
     1381 aacaattcaa aaaagacgtc attattgtcg ctgttggtta ctacctgcgt tacaatctaa
     1441 gctatcgtga agttcaggaa ttgttatatg atcgt
//
LOCUS       DACDMM010000279         1433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.286, whole genome shotgun sequence.
ACCESSION   DACDMM010000279 DACDMM010000000
VERSION     DACDMM010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.286"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            105..1406
                     /locus_tag="IWD16_002782"
     CDS             105..1406
                     /locus_tag="IWD16_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9089751.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          473 a          287 c          264 g          409 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atg
//
LOCUS       DACDMM010000280         1432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.287, whole genome shotgun sequence.
ACCESSION   DACDMM010000280 DACDMM010000000
VERSION     DACDMM010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.287"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(128..418)
                     /locus_tag="IWD16_002783"
     CDS             complement(128..418)
                     /locus_tag="IWD16_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089752.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(536..796)
                     /locus_tag="IWD16_002784"
     CDS             complement(536..796)
                     /locus_tag="IWD16_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089753.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(933..1346)
                     /locus_tag="IWD16_002785"
     CDS             complement(933..1346)
                     /locus_tag="IWD16_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP9089754.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
BASE COUNT          528 a          236 c          212 g          456 t
ORIGIN      
        1 tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata cttcaaaaaa
       61 gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt taatacattt
      121 taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta agagagatca
      181 taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca tcccatattt
      241 cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg atccagcacc
      301 atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag ggaagtaaaa
      361 acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc tcccccacat
      421 tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt gtatgaataa
      481 cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg gaagtttatt
      541 tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat aagccgattt
      601 cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc ttatggataa
      661 aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg aataagtaaa
      721 agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt aagacaatca
      781 aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc gtaaaaacaa
      841 aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa atatataaaa
      901 atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt ttttgtttct
      961 tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt aaaggttcca
     1021 gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga gaccgaatgt
     1081 ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc atgattggcg
     1141 gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc acagccaggc
     1201 atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt tggataactt
     1261 gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc agggaacatg
     1321 tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta ttttttattt
     1381 atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg tt
//
LOCUS       DACDMM010000281         1394 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.289, whole genome shotgun sequence.
ACCESSION   DACDMM010000281 DACDMM010000000
VERSION     DACDMM010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.289"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            33..1372
                     /locus_tag="IWD16_002786"
     CDS             join(33..474,474..1372)
                     /locus_tag="IWD16_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP9089755.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          579 a          181 c          244 g          390 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactgat
     1081 tctacattca gatcaaggat ggcagtatca gatgccacaa tatcaaaaaa aattaaaaga
     1141 gaataacatt attcaaagca tgtctagaaa aggaaattgc ttagataatt ctgtaataga
     1201 aaattttttt ggtgtgctta aatcagagtt tttttatcga gaaaaattcc gatcaattga
     1261 gatatttcaa agtaaattaa atgagtatat taggtggtat aataacaaaa ggataaaact
     1321 aaagttaaat ggactatctc cagtagaata tcggaaacag tccattaaat agttcaactt
     1381 ttggggttca gttc
//
LOCUS       DACDMM010000282         1393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.290, whole genome shotgun sequence.
ACCESSION   DACDMM010000282 DACDMM010000000
VERSION     DACDMM010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.290"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            84..>1393
                     /locus_tag="IWD16_002787"
     CDS             84..>1393
                     /locus_tag="IWD16_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9089756.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVI"
BASE COUNT          497 a          191 c          260 g          445 t
ORIGIN      
        1 tcataaaatc agtataggtg aaaagatgaa ctttcatctg catatcaagt aattggtatg
       61 aggcattttt tgaggtgtag gatatgaagt tggattacct tatagaagag gaagatagag
      121 aaaaactgct gctagctaga tatttagaat ttgagaaaaa gaattatgta gtgctgccta
      181 agttatatga aacattaggt ttgtctaaat ttaaaacaag aaattatatc atagaattaa
      241 ataaagaatt tactgaattc aaaacaaagc cagagatcca aatttcagaa gaagcagaga
      301 tccatgtcga acgtctgaat ttgacggata tcaaaaattt aagaagaatc tactttcaac
      361 gatcaaaaat tgctcgactg ttaacggaga tcgtggattc aaaagtaacg atcgaaaaat
      421 ttgcagagaa tcatttttta agcatatcaa gagcctatgt aaaaagaaga gaattagtct
      481 cttttcttga acagcatcaa ataaagttaa agaaaaacat attagttggg gaagaaataa
      541 acatacggaa cgtattattt ggggtatatt tcggggtctt taatggtttt tctatgccat
      601 ttagagaaag cgtcatgaat gaagcaaaga gtatcgtgca atattttgaa tatctgttca
      661 atttaaaact gtctgcaaca aaacgaatca aattcaattt gctaaccact attagtttgt
      721 tgagggccta tgggaatcaa ccaatcggaa aacgtttctt ttcagatgaa tactttaaga
      781 gctcagaagg aatgaagatc atcaaggatt tatcacatat ttcttttctt catatcccta
      841 aagagcagat cattgatgag atttcttata tcatgttgtt tttgatcacg gaagaagaat
      901 ttgaattaga tggcgcattt ggacttgcgg aagctcattt tcatgatatc cacatgatta
      961 gtcaaaaaat gtcagtggaa ttgattgatc aattaccatt ttcgattgaa atctctgaag
     1021 aagaaagact atatatacga aaagaacttc gtatgggtat gatgcatgtc aatcgaaaaa
     1081 attggatatt cgactttgag atgactagct ttacgtctaa aaagcagcta cagtttttct
     1141 tcgagacgta tccaatattc agtacagcaa tccaaaaggt catacaagag aataaacgct
     1201 atttcttggg aaaagatgaa cgtttcatga ttcgcttatt ttatgattat ctctttttat
     1261 tggtcggtat ttgtccggta tcatattttg aagcaccgat tcatgtctgt attgattttt
     1321 cgcaaggatc tgcttatacc gattacatta tttctcaagt gaaaggattt aaaaattaca
     1381 atattgtgat tga
//
LOCUS       DACDMM010000283         1380 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.291, whole genome shotgun sequence.
ACCESSION   DACDMM010000283 DACDMM010000000
VERSION     DACDMM010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.291"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            501..>1380
                     /locus_tag="IWD16_002788"
     CDS             501..>1380
                     /locus_tag="IWD16_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9089757.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMI"
BASE COUNT          506 a          181 c          224 g          469 t
ORIGIN      
        1 gaaattatgg atcacctttc aaatttagaa aatggaattc ttatacttat tgcgcaacat
       61 ttattgattg caaaattatg tgacaaaaaa aactagtaga agaaagaata atggcttgat
      121 aataaagtac tacctattaa aggtagtact ttatttaatt tagtataaat taacatacta
      181 ctatatttta ggtttcatat tatataaatt aataatatac taccatttgt tggtagtata
      241 atttaaaggt aataagttat tcttttatta ctatgtttgg gttttatagc aaagaaagtt
      301 taataagagt ttggacctag caaacaatat taatgaggtc attaaatctt attatcttta
      361 gtgatataac gaaatatcta cacagacaaa atgatagcgt aaaatatttt gtgtaaatag
      421 atatttaggg tagaaaaaaa gaaagtccat tctgtaaaat taaagttacc acaaccgaaa
      481 ttttatagga ggactttctc atgcccaatt ttactacaga aattatggaa acactaatca
      541 ataaaggtga tttagatgaa ttatttcgat gtcatttaga attagctgtt aattcattat
      601 tgcaggctga attaacagct tttcttgatt atgaaaaata tgatcgtgct gggttcaatt
      661 caggtaattc ccgtaatggg aattattcac gttcattcag aacagagtat ggagaattaa
      721 atttggtaat tcctagagat agaaatggta agttttctca acaaacttta cctgcttata
      781 aaagaaccaa tgactcttta gaaaccacta ttattcagct atttcaaaaa ggaattacta
      841 tgtctgaaat ctctgaattg attgaaaaaa tgtacgggca tcactatacg ccacaaacca
      901 tttcaaatat gactaaaata gtttctgaag atattattgc ctttaaagaa aggtccttag
      961 aatccagata ctctgtcatt tttatggatg ctactcacat tcctttaaaa agacaaactg
     1021 tttcaaaaga agcagtttat attgtaattg gcattcgatt agatggaacc aaagaggttc
     1081 ttgggtttag tcttgctcca actgaatctg cttatgtttg gaaagagatt cttcaagacc
     1141 taaaagaccg tggtttaaaa gaggttttat tagtcgtaac tgatggttta agtggcatta
     1201 acgatagtat tcatagtgtt tatccaaatg ctcaatttca acaatgttgt gtccatatat
     1261 ctagaaatat cgctcataag gttcgtgtta gtgatcgaca agaaatctgt agcgatttca
     1321 aattagttta tcaagcaagt tcaaaagaag aagcaaataa tcaaataagg tttatgatag
//
LOCUS       DACDMM010000284         1365 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.292, whole genome shotgun sequence.
ACCESSION   DACDMM010000284 DACDMM010000000
VERSION     DACDMM010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.292"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(323..937)
                     /locus_tag="IWD16_002789"
     CDS             complement(323..937)
                     /locus_tag="IWD16_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9089758.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          442 a          268 c          230 g          425 t
ORIGIN      
        1 tggggaagac tctttcatca acgtgtatta agagacaaga aagaatccat atggattctt
       61 tcttgtctct ttttccacta ctttttattt ttctgacact acatattctt ataactcatt
      121 tctcataact gtgaaacaac ttataaaaat aacaaacttt aaacaaaaca agcttaaaat
      181 caaagatgat ccatcttaaa ataactaaaa caaaaaaagt agtcgttgtt aatcctcatt
      241 aataatcgtt ttcttttttt caaacgctcc atttttatgt ggaacattat ttttttaagg
      301 aactttgtca acatgtggat aactattttc cacttgtgtc ctggactagc tgttttgctg
      361 taaaatacaa aacttgctgc agcaaaaaga tcgttgcaag agaggctgtt ccgcagatcg
      421 ctggatctag atttggcaaa gtcaagactg catcatgtaa attggcgtgg ttgtccaaaa
      481 ccaaatcagc aaattcaaat aatcgcaaac cactgctatg ttgcgatttg atacttcgtg
      541 acaaatcaaa tccagtaaca atgattaatg gatgaccatt ttctttgatc cactgagcta
      601 gttcgatgat tgccgggttt cttccttcat tcgaaagaag aagaaaaatg tcttctgatt
      661 tgacagaaag ctgatcaata attgcctgtc ctgcaccttc tagcgtttca tagacacctc
      721 cggctggatt ttcaatcaat atacttggta caagatccgg ggtttgatcc caaaattcaa
      781 atgctacacc acttagtgtg cggctagcat atatctgcaa tctaccacct tgattaattt
      841 gtttcgcgat ccaagtgcca gcttgaagca tcagcggctc ttctatcctt aatttctctt
      901 ccaattggtg ataatagtca aaataacata aactcattta tctctcccgc ctagctatac
      961 ttgacatgaa tagaaacagc tgcctaatct aaggaagtac ataaaaaaac agtagttaga
     1021 cagtaacaca tgtggtcttt gctgtttcaa acacaaaaaa ggcattgaac gaatacgccc
     1081 aataccttga catagcaaat aaaacaacgt gcagaccatg gaaaatatct acataggggt
     1141 agaattttca ccctagatta ggtacaataa attaggtcga tgcactgcat tgactatggc
     1201 aaaatgatcg gtcagggtgt atgtgtgtga ctggtcattc cctgattctt agcgccaact
     1261 aagggtcagg gagccttttt ttattcagct gtttctatct gaatttattg taagtaatct
     1321 cttatccaag tgcaagcaag aaaatacaaa aaaacattgg tatga
//
LOCUS       DACDMM010000285         1362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.293, whole genome shotgun sequence.
ACCESSION   DACDMM010000285 DACDMM010000000
VERSION     DACDMM010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.293"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            80..>1362
                     /locus_tag="IWD16_002790"
     CDS             80..>1362
                     /locus_tag="IWD16_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP9089759.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          462 a          255 c          259 g          386 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcacgacaa aataaacaac
       61 tgagaggacg atatgctata tgaatgattc tatcaaaaaa atgctgcgat taatagagaa
      121 agatttgatg attacagagg tctcttacga gacctttcag aagaaaaaga cgttgatcgt
      181 cgatgctgtt ttctcacctg ctccccatac ctgtagaaat tgtggttcta ctgtggtaga
      241 tggaaacggg aaagtaattg tagtgaaaaa tgggaagaag gaaacgattg tccgctttga
      301 acaatacaat cacatgcctt tggttatgcg cttaaaaaag cagcgctata cctgtaaaaa
      361 ctgtcgaacc cattggacgg ctcaaagtta ttttgttcaa cccagacatt caatcgcgaa
      421 tcatgtcaga tataaaattg cttctttact gactgaaaaa gtatctttat cttttattgc
      481 gaaaaattgt caggtatctt tgaccaccgt tattcgtacg ttgaaagagt ttaaaagcta
      541 cttaccaaag caatctaaga ggattctccc cagagtattg atggttgatg aatttcgttc
      601 gcacgcttcc atagaagata aaatgagctt tatttgcgca gatggcgaaa caggacaatt
      661 aattgatgtc ttgcctacgc gtaaattgcc tcgattaaca agctatttct taggctgcgc
      721 taatcctgaa gaagtaaaat ttttagtcac agacatgaac gccgcctact tccagcttac
      781 caaacgtatt ctgccaaatg cgaaagtagt gattgatcgg tttcatatta tcaaacacat
      841 gaatcaagcc tttaatgagt tgcgtatccg tgaaatgaat gaacttcgta aagtaggaca
      901 gaaaagtcag gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa
      961 catcaaccat tatgaataca aaacatggaa aagttttcga gcaccaaaat acccatttct
     1021 tacggaagcg atgatgattg atcgattgct tggcttttct acgtccctga aggaggcgta
     1081 tccctatttt catgaattag ttgaagcctt tcgagacaaa gaccctgact tatttttctc
     1141 cttattggca gaacttcccg aaacgttaga tgacggcttt cgggaaaagc tccaaaacct
     1201 tctgacctat gaagaaggca tcaccaacgc gatgatctat ccttacacca atggaaaaat
     1261 agaagcgaag aatacccata taaagacaat gaaacgagta tcctacggat ttaaatcatt
     1321 tgagaacatg agaattagaa tctttttgat caatcaatta at
//
LOCUS       DACDMM010000286         1362 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.294, whole genome shotgun sequence.
ACCESSION   DACDMM010000286 DACDMM010000000
VERSION     DACDMM010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.294"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            112..>1362
                     /locus_tag="IWD16_002791"
     CDS             112..>1362
                     /locus_tag="IWD16_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9089760.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          426 a          268 c          260 g          408 t
ORIGIN      
        1 ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata gaaaagacac
       61 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
      121 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      181 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      241 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      301 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      361 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      421 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      481 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      541 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      601 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      661 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      721 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      781 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      841 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      901 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      961 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
     1021 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1081 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1141 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1201 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1261 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1321 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DACDMM010000287         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.296, whole genome shotgun sequence.
ACCESSION   DACDMM010000287 DACDMM010000000
VERSION     DACDMM010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.296"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1355
                     /locus_tag="IWD16_002792"
     rRNA            <1..>1355
                     /locus_tag="IWD16_002792"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACDMM010000288         1344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.298, whole genome shotgun sequence.
ACCESSION   DACDMM010000288 DACDMM010000000
VERSION     DACDMM010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.298"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            62..>1344
                     /locus_tag="IWD16_002793"
     CDS             62..>1344
                     /locus_tag="IWD16_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP9089761.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          455 a          261 c          255 g          373 t
ORIGIN      
        1 tatcgttctc tagtatgatt aaatcaccac aaaataaaca actgagagga cgatacgcta
       61 tatgaatgat tctatcaaaa aaatgctgag aataacagag aaagatttga tgattacaga
      121 ggtctcttac gagacccttc agaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc
      181 tactcctcgt gcttgtagaa gttgtggttc tactgtggta gatggaaacg ggaaagcaat
      241 tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt gaacaataca atcatatgcc
      301 tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa aactgtcgca cccattggac
      361 ggctcaaagt tattttgtcc aacccagaca ttcaatcgca aatcatgtta gatataaaat
      421 tgcttcttta ctgactgaaa aagtatcttt atcttttatt gcgaaaagct gtcaggtatc
      481 tttgaccacc gttattcgta cattgaaaga gtttaaaagc tatttaccaa agcaatctaa
      541 gaagatcctc cccagagtat tgatggttga cgaatttcgt tcgcatgctt ccatagaaga
      601 taagatgagc tttatttgcg cggatggcga aacaggaaaa ttaatagatg ttttgcctac
      661 gcgtaaatta cctcgattaa caagctattt cttaggttgt accaatccag aagaagtaga
      721 attcttggtg acagacatga acgccgccta cttccagctc accaaacgtg ttctgccaaa
      781 tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac atgaatcaag cgttcaatga
      841 gttgcgtatc cgtgaaatga atgaacttcg taaagccgga cagaaaagcc aggcagaaaa
      901 actgaaaaag aactggcgct ttttgctaaa aaatcgtgca aacatcaacc attatgaata
      961 caaaacatgg aaaagtttcc gagcaccaaa atacccattt cttactgaag caatgatgat
     1021 tgatcgattg cttgagtttt ctccgcccct aaaggaggcg tatccctttt ttcatgaatt
     1081 agttgaagcc tttcgagaca aagaccctga cttatttttc tccttattgg cagaacttcc
     1141 cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac cttctgacct atgaagaagg
     1201 catcaccaac gcaatgatct atccttattc caatggaaaa atagaagcga agaataccca
     1261 cataaagaca atgaaacgag tatcctacgg atttaaatca tttgagaaca tgagaattag
     1321 aatctttttg atcaatcaat taat
//
LOCUS       DACDMM010000289         1323 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.299, whole genome shotgun sequence.
ACCESSION   DACDMM010000289 DACDMM010000000
VERSION     DACDMM010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.299"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..300
                     /locus_tag="IWD16_002794"
     CDS             <1..300
                     /locus_tag="IWD16_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9089762.1"
                     /translation="DLDDGVGEYSKMGVCSLDTSFRLLLLDKEQRKNDYKLQLDSDIG
                     DVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPSGSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(414..1043)
                     /locus_tag="IWD16_002795"
     CDS             complement(414..1043)
                     /locus_tag="IWD16_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9089763.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1177..1249)
                     /locus_tag="IWD16_002796"
     tRNA            complement(1177..1249)
                     /locus_tag="IWD16_002796"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1214..1216),aa:Thr,seq:ggt)
BASE COUNT          441 a          206 c          231 g          445 t
ORIGIN      
        1 gatttagatg atggcgttgg ggaatattct aaaatgggtg tttgctcttt agatactagt
       61 tttcgtttac ttcttcttga taaagaacag agaaaaaatg attataaact ccaattagat
      121 agtgatattg gggatgttta tataaaagat tattcaaaaa tgtacatgga tgaacatatg
      181 accctttctt tagatccacg tctaggtgta tttactcttg aaagcccaag tggttcttta
      241 gacagtcatg ttcaattagt agatctacga ccagaaaaat tctctggaat aaaacaataa
      301 ctttctctct attgtccata aaactagtct caatcaagct gtattgaaaa aacaagacaa
      361 cattcttacc atggtaaata aaaagacgac acattttgtc gtcttttttt aaattaacta
      421 ttttcgataa aagatcgcat agcttgtgcc attatttctg gcttttctct attcaattca
      481 tgttttccgt ttttaataaa gagtagccgg ccattcgttg aaagtttata taaacgtttt
      541 gctgctggtt tattcgcatg atctttggta ccgcataaaa tcaatttagg aagtcgtgat
      601 gtacgaagct gttttttgca gtctaatgtt tcaagtgaac gaagtagggt aagggtttgt
      661 tttttagata gacctaattt ttcaaatatc ttagaaggta cgcagtaaaa ataaatattc
      721 tggaaagtaa gcaataagcg attaggagac tcaaattgtg gagcgactaa aacaaccttt
      781 tttaaatatg gagaaggagt tgcaagatag tttaaagcaa taacagcgcc taaagataat
      841 ccacataaaa taaaaggttc ctttatctga ttcaattgta cttcgatgta tttggttaag
      901 tcgtctaaag tagcagtgtc tgtgctgttt aaatgattga acaatgaaag acttttgcaa
      961 gaataactat tgggtagata agagattaca tctttccatg attgttcgtc ttggcctaga
     1021 ccatgcaaaa aaacaatttt catattgctc tcctttttat agttatcata tagactttct
     1081 ttgtactatt aaaatataaa cattcatttc ttacgtcata aaataaaaaa acagcgcaga
     1141 cacattgtta aaccaacatg ttgcactgtt taaggaagcc acttgccggg cttgaaccgg
     1201 cgacctcttc cttaccatgg aagcgctcta ccagctgagc taaagcggca ttatcattaa
     1261 tacaaataat aggataacga atattaatgt atttgtcaac agaatttttt cgaaaaaatg
     1321 tgt
//
LOCUS       DACDMM010000290         1312 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.300, whole genome shotgun sequence.
ACCESSION   DACDMM010000290 DACDMM010000000
VERSION     DACDMM010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.300"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            202..1077
                     /locus_tag="IWD16_002797"
     CDS             202..1077
                     /locus_tag="IWD16_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089764.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..>1312)
                     /locus_tag="IWD16_002798"
     CDS             complement(1222..>1312)
                     /locus_tag="IWD16_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP9089765.1"
                     /translation="LLQNVEKYHKIYQQCRKIYPATKEICHDL"
BASE COUNT          475 a          240 c          218 g          379 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga ga
//
LOCUS       DACDMM010000291         1294 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.301, whole genome shotgun sequence.
ACCESSION   DACDMM010000291 DACDMM010000000
VERSION     DACDMM010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.301"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1244
                     /locus_tag="IWD16_002799"
     CDS             <1..1244
                     /locus_tag="IWD16_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9089766.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DACDMM010000292         1260 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.302, whole genome shotgun sequence.
ACCESSION   DACDMM010000292 DACDMM010000000
VERSION     DACDMM010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.302"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(76..1170)
                     /locus_tag="IWD16_002800"
     CDS             complement(76..1170)
                     /locus_tag="IWD16_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9089767.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT          368 a          270 c          196 g          426 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
//
LOCUS       DACDMM010000293         1259 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.303, whole genome shotgun sequence.
ACCESSION   DACDMM010000293 DACDMM010000000
VERSION     DACDMM010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.303"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            338..916
                     /locus_tag="IWD16_002801"
     CDS             338..916
                     /locus_tag="IWD16_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9089768.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
BASE COUNT          411 a          202 c          240 g          406 t
ORIGIN      
        1 ctttgatcca gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa
       61 accataaaag cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta
      121 gcactaaaag aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc
      181 ctcttgttca ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa
      241 ctgaagactg atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt
      301 tatcatctaa aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc
      361 atttacagat gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc
      421 taacggagac acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt
      481 cagttttatc attttagcga tttattggaa caatcatcat catttatttc aagttgtaca
      541 taagataaat ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt
      601 tccgtttgca acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta
      661 tggttttgtt accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa
      721 agcaaataaa ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta
      781 tctatcgatt ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc
      841 cgtgctgatc gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga
      901 atcacaatat cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata
      961 aataaacgat caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa
     1021 aaagaattca tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag
     1081 gtcatttgac ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa
     1141 aaaaataaac atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag
     1201 cagtatgatt ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagt
//
LOCUS       DACDMM010000294         1257 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.304, whole genome shotgun sequence.
ACCESSION   DACDMM010000294 DACDMM010000000
VERSION     DACDMM010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.304"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            12..1091
                     /locus_tag="IWD16_002802"
     CDS             12..1091
                     /locus_tag="IWD16_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9089769.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          372 a          300 c          308 g          277 t
ORIGIN      
        1 gatgaattca catgacgcaa acccatgata acacagtttc acgcaaggga aaacatcttt
       61 cctactcaga acgttgccaa atcgctatcc tgaaaaagga aggctattcc aaccgtcagg
      121 ttgccaaagt gctgggacgt gttccgcaaa ccataaacaa tgagattgat cgcggaacca
      181 tcacccagtt aaaacgccaa acgcaaaatg gaaaagtgta tgacacctac tattctgttt
      241 atgacgctga tgcaggacag gccttctatg aaaagcaacg actgaactgt ggcagacgcc
      301 ccaaatgggc gaacacaaat gccttcattg attgggcaga tgacaaaatg ttaaatgaaa
      361 aatggtcgcc ggacgtcgtg gtcggatttg ccagaaaaca cgagctgttc gattgtgcca
      421 tcattcctgg cacaaccgct ctttatcaat ggattgaccg cggaatcatg cgaacaaaga
      481 acatggactt gcttgaaaaa ctgtcccgta aaatcaaagc ggtccgccca ggacaccgtc
      541 ccaacaagcg tgttctcggg acgtcaattg aagaacgccc taaagaagtt gaaacacggg
      601 aaacgttcgg tcattgggaa atcgacacgg tagtgggaaa caagaccaag tcggatgccg
      661 tgctgctgac gctggctgaa cggcagacac gctttgaagt catcctaaag ttgaacggga
      721 aggacaccga atcggtggac caagcggttc aacagctaag agaacgggct ggcgctaact
      781 tcgatcaatt atttaagacc gttacatccg acaatggctc ggagttttct gggctacacg
      841 atgctctgca agatgtagct gacgtctatt tcagccaccc ctatgcttct tgggagcgtg
      901 ggacgagcga aaaccagcac aaattaattc gccgtttcgt ccccaaggga agcccgatta
      961 gtgaggtgag cgagaaccag attttgcgga tccagcgttg gatgaacgat tacccgcgga
     1021 aaattttggg ttatgcgaca ccacatgata cattcgtcca ggcattcaag caagaacggt
     1081 tggtggcttg acccattatt ccttgataac gatcctcact cgggacatgg cttcatgtct
     1141 ggaaagctgg cctggggcca gaatcgtctg aagactatca tacctcagtt cattatcaag
     1201 gcctcactga tttagaataa cgaggcagtt acggtatcaa acaaatgagt tacctgt
//
LOCUS       DACDMM010000295         1241 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.305, whole genome shotgun sequence.
ACCESSION   DACDMM010000295 DACDMM010000000
VERSION     DACDMM010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.305"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            66..632
                     /locus_tag="IWD16_002803"
                     /pseudo
     CDS             66..632
                     /locus_tag="IWD16_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            745..>1241
                     /locus_tag="IWD16_002804"
     CDS             745..>1241
                     /locus_tag="IWD16_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP9089770.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          462 a          196 c          220 g          363 t
ORIGIN      
        1 ttgataaaaa aaatactaga tgtaagataa aagtgtaaaa acgatttgtt ttaggagggg
       61 aaacgatgtg taccgcaatc acttatgcaa cgaaagacca ttattttgga aggaattttg
      121 attatgagat gtcttataat gaagtggtca ctattactcc aagaaattat cgtttcgatt
      181 ttagaaaggt aaaaaattta gataaacatt atgcaatgat aggaatagca gctggggtat
      241 ccaactatcc actctattat gaggctacaa atgaaaaagg gttgagtatg gcaggactga
      301 attttcctgg caatgctgat tataaagaat tacaagaagg aaaagataac gttgcaccat
      361 ttgagtttat tccatggata ctagggcaat gctcaaccat agacgaagct aaagaattat
      421 tggctactat caatctagtc aatatcgatt ttagtgaaaa actacccttg tcacctttac
      481 actggttatt agctgataaa gaaaaatcga tagtaattga aagtatgaaa gatggacttc
      541 atttatatga taatcctgtc ggtgtgttga caaataatcc gccatttgat tatcaattat
      601 ttaatctgaa caattatcgc tcgctatcaa atggaacgag agtataaaat attttgtgta
      661 aatgaaaaaa tccatacaaa aaaggaagtc ccttctgtag aataaagtta acgacaacca
      721 attcacagaa aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta
      781 gtcactaaag gcgatttaaa tgaattattc cgttcgcact tagaaaaagc gataaacaca
      841 ctcctacgga ctgaattaac ggctttttta gattacgaaa aatatgatcg cactggtttt
      901 aattcaggta attcgagaaa cggttcttac tttcgatcaa tcaaaaccga atatggtgaa
      961 ttaacattgg aaatacctag agatcgtaat ggtgagttta aacaacaaac tttaccagcc
     1021 tacaaaagaa caaacgatac attggaaacc actattatcc atttattcga aaaaggtgtt
     1081 acgatgtctg aaattgctga tttgatcgaa aaaatgtacg gtcatcacta tactccacaa
     1141 accatgtcca acatgactaa agttctgact gaagaagtaa atgcctttaa atccagagcc
     1201 ttaaatgata agtatgtcgc tatttttatg gacgctactt a
//
LOCUS       DACDMM010000296         1231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.306, whole genome shotgun sequence.
ACCESSION   DACDMM010000296 DACDMM010000000
VERSION     DACDMM010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.306"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1231
                     /gene="aph(2'')-I"
                     /locus_tag="IWD16_002805"
     CDS             <1..>1231
                     /gene="aph(2'')-I"
                     /locus_tag="IWD16_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP9089771.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIETIV"
BASE COUNT          513 a           98 c          211 g          409 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     1141 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     1201 tgaagaatat tatcctattg aaactattgt t
//
LOCUS       DACDMM010000297         1219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.307, whole genome shotgun sequence.
ACCESSION   DACDMM010000297 DACDMM010000000
VERSION     DACDMM010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.307"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..931)
                     /locus_tag="IWD16_002806"
     CDS             complement(<1..931)
                     /locus_tag="IWD16_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP9089772.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIF"
BASE COUNT          485 a          199 c          144 g          391 t
ORIGIN      
        1 taaaaattaa aacaatacca agaaacgcat aattcacttt acttctaaaa aatgttttga
       61 gtaaactaaa gatatttaac acatagaaag catagataca tataaaagca attataccac
      121 ttgatacaag tacttgaaga aatccattat gcatgtttcc atgtgctctt tttagttcat
      181 tcacatctgt agtatcaaga tctacttttt tcaaaaattt tgcatcgcta ttcccataga
      241 aatcggtctc tccataacca aataacaaag ttggtcttat aactttaagc gcagctttcc
      301 aaatcgtaaa acgaccagct gaaatttcgg agttatcact agaatgctgt aatattactt
      361 ctttttctgg aagtggagtt ttatctgatt catttgactc tataacttct acttttctat
      421 caatcgatac aggaattgtt gctaaaaaat ccttagcata attagtacca aaagaaaaat
      481 ataccaatag aactaataat atgcttacca tccatttgat ttttacaggt agtacttctc
      541 taatgctctt tctaaatttt gaattggcaa acaacaaaag aaataatact aaaaaagaac
      601 aaattactac ctgggcacct ctagacgaag aaagtgtaat ataaaatatt tgtacgataa
      661 aattcatcat ataaaaaaca cgaatcgttt ttgttatttt tgaaattaat acactaatta
      721 aacttaaaat aatagaagta tatccaaaca tagctccaac atttggactt gtatagacac
      781 caaataaccg atgttcaata aaaccttgtc ttgcaagtaa tcctggcgtt cctgttggga
      841 catgataaga aatcaaaaaa ataaattgat agatagatat tagcgcagct aaaaaaatta
      901 tgataatgaa aacattatta aaatacacca tttttttctt tttttcctct tcactttgtg
      961 aaaagtctgc aggaaaaatt actatcaaag taatcagcaa atatccaaaa ttataaatag
     1021 atggaattaa aaaattttct ctgttaatga ttatagttaa gataaaacta atcattgctc
     1081 caatcagtag ccaagaaaaa cgcattttaa aaaaattttt ctttataaaa acctcataca
     1141 aaactataat tccaccccaa gcaaaagcta ttttcatcgg gatattagtt acattaaaag
     1201 tactaggcat aaaataaag
//
LOCUS       DACDMM010000298         1218 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.308, whole genome shotgun sequence.
ACCESSION   DACDMM010000298 DACDMM010000000
VERSION     DACDMM010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.308"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..895)
                     /locus_tag="IWD16_002807"
     CDS             complement(<1..895)
                     /locus_tag="IWD16_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9089773.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYD"
     gene            complement(901..>1218)
                     /locus_tag="IWD16_002808"
     CDS             complement(901..>1218)
                     /locus_tag="IWD16_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9089774.1"
                     /translation="YQAFDVLMLPSLFEGVPLVGIEAQFADLPCFFSEKVPTEVAFSD
                     KTNFISLNQSAKEWAREIANVDISHRDSERSILIKADYNIQTAYKILESKYYELFELI
                     QRG"
BASE COUNT          378 a          223 c          148 g          469 t
ORIGIN      
        1 catcatatat attatttaaa tttttaatca attcgtcaca taatggattg ttactaggta
       61 ttttatcaaa tgtctgtgct atttcatgat ttattctctg tgctaaaaca atcatataat
      121 ctatcatcaa ataatccacc atcatatcat tatttttcca ataatttaaa aaaaagtctc
      181 taattatagc aaacatccat ctagactctt gattacatgc taatgaataa cccgcaaaat
      241 atcctgatgc tacggatgcg tgagcataat caggcctcct aatagaccaa atatcgatat
      301 caaaatactc atctggtatt ggttttgtac aataaaatgt ggcgtctaac cacaaacctc
      361 catgttttgc tagcaaagat aatcttaata aatcagaata atttgttctc gtaatgattc
      421 ctttgttttt cttttcttcc acccattttg gtatatcaac atattgtcta taattatctt
      481 ctgttaaaat tataacagta tgtttccctg catttcgttt tattgatttt acacaagctt
      541 ttacaatttc cggcgattga tccaatccct gccaccaaca aacccaaatg cagttagaat
      601 atggtgattt tgattgatta attatttgat tttgactata atcatatgtt ttcaaaaaat
      661 ctgaaaaagt cttttcataa tacttcagca ttacctcatg tttttttaat aaatgttcct
      721 tctgtttttt actttcatta tatccattac gattcatgac ttgaatatca attttagctt
      781 gtaacgtggc aaatgcttcc ttccaagatg ttatctttgc aattcgtctt gttgcattca
      841 attcttcgga tatgcgatgt ataattcttt ttatacttga atgtttctgt gtcatattct
      901 ttaacctctt tgtattaatt caaataactc ataatatttt gattctaaga ttttgtatgc
      961 tgtttgaata ttataatctg cctttattaa aatcgaacgt tctgaatctc gatgagagat
     1021 atctacattt gcaatctctc tcgcccattc ttttgccgat tgatttaaac ttatgaaatt
     1081 ggtcttatca ctgaaagcaa cttctgtagg aactttctcc gaaaagaaac acggtaaatc
     1141 tgcaaattga gcttcaatac ccactaacgg aaccccttca aataatgatg gcaacattaa
     1201 tacgtcaaat gcttgata
//
LOCUS       DACDMM010000299         1161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.310, whole genome shotgun sequence.
ACCESSION   DACDMM010000299 DACDMM010000000
VERSION     DACDMM010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.310"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            14..1141
                     /locus_tag="IWD16_002809"
     CDS             14..1141
                     /locus_tag="IWD16_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9089775.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          419 a          201 c          224 g          317 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa c
//
LOCUS       DACDMM010000300         1143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.311, whole genome shotgun sequence.
ACCESSION   DACDMM010000300 DACDMM010000000
VERSION     DACDMM010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.311"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWD16_002810"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWD16_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP9089776.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DACDMM010000301         1127 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.312, whole genome shotgun sequence.
ACCESSION   DACDMM010000301 DACDMM010000000
VERSION     DACDMM010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.312"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            365..598
                     /locus_tag="IWD16_002811"
     CDS             365..598
                     /locus_tag="IWD16_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP9089777.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            595..1002
                     /locus_tag="IWD16_002812"
     CDS             595..1002
                     /locus_tag="IWD16_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9089778.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT          424 a          145 c          194 g          364 t
ORIGIN      
        1 ctattttttg atatatttct gtatttttca tgtcattcat cctttcaata agtaggggag
       61 aagagtatct ctataattgg actataccaa ttatagaatg ttttgtcaat tggtatgctc
      121 taaaatttat taatttgttt caaaaaaagc atataaacag gtatacgttt aataatttaa
      181 aataaatggt atacataaat agggaaggaa atagaaggga gcaatctaaa ttttttgaat
      241 tttcttaatt aattatatag aaagaacaat ctttctatag agttagacat gagagggact
      301 atgcttgatt taaaatcaac aagcgtatat actgtatata caaagaaaaa ggagggagaa
      361 gactatggca attaaagaaa gaaaatttag ggaagtgggg aactcagtag tattaactat
      421 ttcaaaggag tttcttaaag aaaatgggtt aaatcccgga gatacggttt ttttagatga
      481 agaaaaactt cgagcggcaa ttacaaaaaa acaagcctta tctgatgaaa ttgatttagc
      541 aatatctcaa gcattaactg aatatgatac tacttttaga gagcttgtcg ataaatgagt
      601 attcattacc tagaagccaa agatattata aaaatgaatg taatccaaat aaaaaaatac
      661 tctcctaatg aaccccttgg tgtaaaggat gcgaatgctt tagaaatgtg tgtagggcaa
      721 cttcgagcct cagcttttgg tgaagaggtt tatccttcga tttatgaaaa agcagctatc
      781 ctacttatac aattgataaa gaaacatcct tttcacaatg caaataaacg tactgctttc
      841 ttagctactt ttgtcttttt gaaaatcaat ggacagcttt tagctattga tcaaaaagat
      901 gctgtaaatt tagcggtata tattgctacg tatgataagg atttcgatca actaaaatat
      961 gaagtgatca acactataaa gacacatacg cagcccattt aaggagttac tgtatgtgtc
     1021 tttatagggt tattagctgc tatagcgaaa taattgaagt aaattacaaa tagctataat
     1081 tcaaatccaa ctatatagaa aacgtaaatt ttctatatag ttggatt
//
LOCUS       DACDMM010000302         1121 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.313, whole genome shotgun sequence.
ACCESSION   DACDMM010000302 DACDMM010000000
VERSION     DACDMM010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.313"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(235..1116)
                     /gene="galU"
                     /locus_tag="IWD16_002813"
     CDS             complement(235..1116)
                     /gene="galU"
                     /locus_tag="IWD16_002813"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9089779.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          283 a          253 c          177 g          408 t
ORIGIN      
        1 cttggctttc atttctctta tgtaatcatt ttcttttata caacttaaaa aaaaattaaa
       61 ccattagcat aatattttct gaatctattc acgaaagtct ttttctatct ttcgttttcc
      121 acgactcaac tgatctagag tcaaaaaact aaaacgacag ttgcgcaaat gacgcacaag
      181 ctgccgcttt aataacaaat agcctttcat tctcagcagg ccacttattt ccagttaact
      241 ttctgttctt agctttttac tcaactggat aatatatgca cttaatgaag tcccaatttc
      301 cgaatgttga agtccatatt caatatttgt cttcatatat cctaacttat cgcctacgtc
      361 gtatcttttt cctttaaact ctctcgcata gaccgcctct gtctggttaa gagtctgaag
      421 tgcatccgtt agctgtatct cgtttcctgc tcctggttct tgagtttcta atatctcaaa
      481 tattttagga gtcagtagat aacgtccgat aatggctaaa ttacttggtg cttttcccat
      541 ttcaggtttt tctacaaacc cttttactct gcaacgaccc ttttcgctat ctgttttagg
      601 atcaataatt ccgtatttag atgtttcatt ttctggtaca cgcatgacgg caacagcgga
      661 agcttgtgtt tcttcataat ccatcatcaa ttgttgagtc aatgggatat catctttcat
      721 caaatcatcc cccagcatga cgacaaacgg ttcatttcct ataaatgctt ttgcttgaag
      781 cacggcatcc cccagtcctc ttggatgaga ttgacggatg aagtgaagat tgacttgagt
      841 cgtttcttca actaaagcga gtaattctgt tttttccttt tcccgcaaat tgttttccaa
      901 ttcgatattt gcatcaaagt gatcttcaat gggtcttttt tcttttcccg ttacgatcaa
      961 gatgtcttcg attcccgaat ccaatgcttc ttccacaata aactggatcg ttggtttatc
     1021 cacgattgga agcatttctt ttgccatggc ttttgttgcc ggcaaaaaac gtgttcctag
     1081 cccagcggca ggaatgactg cttttttgac cttcacttgc t
//
LOCUS       DACDMM010000303         1117 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.314, whole genome shotgun sequence.
ACCESSION   DACDMM010000303 DACDMM010000000
VERSION     DACDMM010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.314"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..900)
                     /gene="vanY"
                     /locus_tag="IWD16_002814"
     CDS             complement(<1..900)
                     /gene="vanY"
                     /locus_tag="IWD16_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9089780.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTN"
BASE COUNT          329 a          228 c          159 g          401 t
ORIGIN      
        1 attagtatgt gttgatccgg gaaacactgt cacaattaca ccgtctatat tgtttcctga
       61 tatctcataa cgaagattag tcggcacatg aatggtggta tttttagtaa caggataata
      121 aaagatctca tatttttccc catttacact aacagaaatg gttttttctt cttttaggta
      181 atccatatat tcctcgagaa cgaaattctt ttctttcata atcgcactat gtggtaaacc
      241 aacatagcga atatgccatg gttcatattg aattcctgtt aactctgttt tgtcctctgg
      301 ataacgtaaa atgaacccgt atttccaagc attttcttct atccactttc cttcaggggc
      361 tcgttccatt ttcgtcaagc ttgatcctac atctagtgat aaacctgaat tatgctcact
      421 ataacctgct ggtaaggcat actcagcccc catttcttgg taaagcacac tttgctcatc
      481 aaagtctcga tagccactat taataataaa atgactaacg ccacccttta cagcatcatt
      541 gaccatctct gaaaattttt gtgctatttc ttttgacata taaatattac tatcaagcaa
      601 cccgtatcca tttattaatt cgtcatgttt agataaattc acgatatctg acttcacact
      661 ttcttggcga acaggatatt tactattgat taatagcaga tttccttgat aaacctgttc
      721 ttctgtaatt gttttctctt gggtattttc agaagtccca ctattttcta aatgttcttt
      781 gggattttga tcataatttt gaaattcgac tttttcctga gaaaacagtg cttcattaac
      841 gtagtcataa cctaagtata ttaagaataa caataacaat aaaaaaaaca acttcttcat
      901 tttcagtctc ctccttaact atatagttgg aagtatgaaa tatttctaga atcttcccct
      961 taaacggcac aatctcgttt gagattgtgc cgttttgccc cgtaaattac agtcaattat
     1021 ttcgccctac tggatttact attatcacca atgtagatga attttcataa gtgataccca
     1081 tgctgaattt aacgaggctg aactgcctgt tgacaat
//
LOCUS       DACDMM010000304         1096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.315, whole genome shotgun sequence.
ACCESSION   DACDMM010000304 DACDMM010000000
VERSION     DACDMM010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.315"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..405
                     /locus_tag="IWD16_002815"
     CDS             <1..405
                     /locus_tag="IWD16_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9089781.1"
                     /translation="KPSNMYVFLVLTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQR
                     EDGTIYAVYSFARKIGQALAGGVGGWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAV
                     CFFVVGLILFFVYPLSKEKVLENYNLLNQKHN"
     gene            416..>1096
                     /locus_tag="IWD16_002816"
     CDS             416..>1096
                     /locus_tag="IWD16_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9089782.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHRDKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEK"
BASE COUNT          391 a          126 c          219 g          360 t
ORIGIN      
        1 aagcctagta atatgtatgt attcttagtt cttacttctg ttggatatat ggggttaggt
       61 atttttaata cagtcatttg ggcgaatatt actgatgtaa tcgatgatca agaagtgaaa
      121 tctggccaaa gagaagatgg aactatttat gcagtatatt cttttgcccg aaaaattgga
      181 caagctttag ctggcggtgt cggaggttgg actctttcta taatagggta tgattcgtta
      241 gcaaaagtac aaagtggaga agttttacaa aagctgtata actcttcaac gttgattcca
      301 gcagtttgtt tctttgttgt aggattgatt ttatttttcg tatatccatt atctaaagaa
      361 aaagtattag aaaattataa tttgctaaat caaaaacata actaggagaa aaaacatgaa
      421 cagttggata ttttgtattg attcagatgg ttgtgtcatg gatacaatga cttataaaca
      481 caatctcttt ttcgggccaa ttgctgcaga gatttttgat gttcatcata gggataaatt
      541 cctacaggat tggaataata ttaatttata ttcagagact cgtggaataa acagattttt
      601 aggtttagtt aaaacattag aaaaaaatga tgtaccatcg attaatcgat taaagcactg
      661 ggtatctgta acaaaagaat tgtctaatcc atctttgaag agggaaatag aatactctca
      721 gtcagaggat ttattaaaag ctttaaaatg gtcggaagaa gtcaatcgac agattagtca
      781 agctaaagga ttagataaac cctttttaag tgctagagat actataaaag ctctgaaaaa
      841 atatgggaag ataattattg taagctcagc taataaggaa gctattcaag aagaatggga
      901 aagacatgaa ttattatcct tagttgatga actgtgttgt caagataaag gaaaaaaaga
      961 agacattatt cgaagtgtaa ttgaaaatgg ttgtgatttg gataagatat taatgattgg
     1021 tgattctcct ggtgatctag aagctgcaaa taagaataaa gttttctttt atcctatttt
     1081 ggttaataaa gaaaaa
//
LOCUS       DACDMM010000305         1092 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.316, whole genome shotgun sequence.
ACCESSION   DACDMM010000305 DACDMM010000000
VERSION     DACDMM010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.316"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..616
                     /locus_tag="IWD16_002817"
     CDS             <1..616
                     /locus_tag="IWD16_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089783.1"
                     /translation="GLKRIELLALSGAILVYYVLLKWGKKIQIRALVCTICATVISLV
                     FIYIIDTGILALLMSNLGITDDGARLSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLY
                     LSHFRLNGGRIAASLHSDILTIYIEVGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECV
                     LLLTIYMFILYLTDNTLTYPDTQMIFLLVPMIVSWVDNQQRISE"
     gene            757..>1092
                     /locus_tag="IWD16_002818"
     CDS             757..>1092
                     /locus_tag="IWD16_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089784.1"
                     /translation="MKNTIFSDEYVEIIISDHSKNTKEIAKKIEEKKVFDGCHYLESK
                     SFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFDEFICFSMGEVWLEGIYACL
                     YRNNKNVQVS"
BASE COUNT          381 a          104 c          203 g          404 t
ORIGIN      
        1 gggattgaag agaattgaac tgttagcttt aagtggtgca attctagtgt attatgtttt
       61 attaaagtgg ggaaaaaaga ttcaaatccg tgcattagtg tgtactattt gtgcaactgt
      121 tatttcgtta gtgtttattt acattataga tacaggaatt ttggcattgc ttatgtcaaa
      181 tttgggcatt acagatgacg gtgcgaggct ttcattttat ctatattcat ctaaatttta
      241 tgaacttggt ttaggatatg taggaacagg atggacatgg ttttcaaaat acttttttaa
      301 tctttattta tcgcacttta ggttaaatgg tggaagaatt gcagcaagcc tacacagtga
      361 tatattaact atttatatag aagttggatg tatagttttt attatatgga tgttttggtt
      421 attttttgtg aaaccgataa gactaactaa aaaatttgga ttgaaaacag gggagtgtgt
      481 attattatta actatatata tgtttatatt atatttgacg gataatacgc tcacttatcc
      541 agacactcaa atgatttttc tcttagtacc tatgatagtt tcgtgggttg ataatcaaca
      601 acgaatatcg gaatagatag ctaaagtata ttttaattat gaatttataa ttagttgtaa
      661 aagttaatat ttaacggaca ggaagtaggg tgaaaaaatt tgaaaaaaat tttagtgtta
      721 gcaaatacat attatcaaat aatagtagca atacaaatga aaaatacaat tttttccgat
      781 gagtatgttg aaattattat ttctgatcat tctaaaaaca ctaaagaaat tgcgaaaaaa
      841 atagaagaaa aaaaagtgtt tgatggctgt cattatttag aaagcaaatc ttttgatgat
      901 aatattgggt attttcaaaa gattaaggac tgtcttcaga tttcttgtgg taaatcaaat
      961 cgatatgcct attatttgaa aaactgtaaa agtgaatatt ttgatgaatt tatttgtttt
     1021 tcaatgggag aggtttggtt ggaaggaata tatgcttgct tatatcgaaa taataaaaat
     1081 gtacaggtat ca
//
LOCUS       DACDMM010000306         1090 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.317, whole genome shotgun sequence.
ACCESSION   DACDMM010000306 DACDMM010000000
VERSION     DACDMM010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.317"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..302)
                     /locus_tag="IWD16_002819"
     CDS             complement(<1..302)
                     /locus_tag="IWD16_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP9089785.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
     gene            complement(522..920)
                     /locus_tag="IWD16_002820"
     CDS             complement(522..920)
                     /locus_tag="IWD16_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9089786.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          345 a          216 c          159 g          370 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atgggacaaa ataaaaaggc
      361 aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc gaacggcatt
      421 catcacccac ctatagagga tgggcgactt ctgcctaatt ctgttaaatc catacgtcct
      481 aaaacaagct gtttttgctt aatatgatca tctgagagac atcaaatcaa cttttctctc
      541 aacaaatcat tcaaagcttt cgacagatta atattctctt cagccgctaa aacatttaaa
      601 tagtgttcca cagtcactga ttttcgatca taattttgct tgtttcgagc tttagcaact
      661 ttcgtattag cttttacgac aaataaatta acattctcag atcgcttgat tcttgcattt
      721 aactcttgaa caggcgtagg cgatggaatt tctttttctt gcaactctaa aagcaccaag
      781 tatccttcca atacttcttg cgccatctca aaagcatctt ctaaagtttc accatgtgta
      841 tataagtctg gaaaatctgg gaatttaact gcatattcat ttcctttcca ttcgaatact
      901 gcacaaaaaa agtatttcat cgcattgcac ctctctaatt aaaaaataat ttttgctttt
      961 ttgtttcaaa aaaaagaatc gcctaccaaa aaaattgata ggcgatcaca agctaattaa
     1021 cctctaaagg agattaatac cttgctccca aggcactata tatcttagca tacgtctgaa
     1081 gcatcttcaa
//
LOCUS       DACDMM010000307         1074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.318, whole genome shotgun sequence.
ACCESSION   DACDMM010000307 DACDMM010000000
VERSION     DACDMM010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.318"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            161..271
                     /locus_tag="IWD16_002821"
                     /pseudo
     CDS             161..271
                     /locus_tag="IWD16_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(368..844)
                     /locus_tag="IWD16_002822"
     CDS             complement(368..844)
                     /locus_tag="IWD16_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9089787.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(857..>1074)
                     /locus_tag="IWD16_002823"
     CDS             complement(857..>1074)
                     /locus_tag="IWD16_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HAP9089788.1"
                     /translation="ALGGKENIESVEACATRLRVAVTNGEIIQKQTIKDTGATAVFEV
                     KGGIQAVFGGKADLLSQEINQILGKDN"
BASE COUNT          350 a          200 c          138 g          386 t
ORIGIN      
        1 catctttcag cattttacta tatttggtat atagtgctaa ggttcatatt cgattgacaa
       61 gagaatataa attgatctat ttctatttcc taagttgagt aaacataact ttgcccttta
      121 taaatatctt ccctctctct cttctctcta tattttatat ttgaataatc atatcaaggt
      181 attccaacga aatgccaatg gatttcgaag cttttataac tttaaattac ggatcatgat
      241 ttggcatggg gaatcattac taataaaata aaaaaatgaa cagaaagaaa acagttcttt
      301 ctgttcacca acttcatttg acaaatatcc tatttagctt attttatagc tttttatata
      361 cactttttta aaaagtgatt tttccacatg aattcccatg agcaattcga ccattatcta
      421 ttattaaatc attgaactga tttttctccg gaaaaacaac tataacagaa gtatctttac
      481 cagattttat tattttgtca aaatccacat caaccaacga ggtttcagga gtaacctcat
      541 cattttcttg aacacaaatc gaaaaaggct ctccttgtaa ttcaactgta tcaattccaa
      601 tatgaatcaa ataatcaatg cctgagttgc tttttatatt aatagcatgt ttagtaggaa
      661 atatatttgt tactcgacca gccaatggag cataaatttt tgaaccatca ggtatgacgg
      721 caaaaccatc acctagtaat ttttgtgaaa agacaggatc atttacttct gtgatggaaa
      781 tgatttttcc ggaacaaggt gcataaaaag tttcttgttt tttctttttg aaaatttcaa
      841 acatattaaa aacctcctaa ttatcttttc ctaatatttg attgatttct tgacttaata
      901 agtcagcttt ccctccaaaa acagcttgga tgccaccctt cacttcgaag acagcagttg
      961 caccagtgtc ttttattgtt tgtttttgaa taatctcccc attcgtaaca gcaacacgca
     1021 aacgagttgc acaagcttca acagactcaa tattttcttt tccaccaaga gctt
//
LOCUS       DACDMM010000308         1074 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.319, whole genome shotgun sequence.
ACCESSION   DACDMM010000308 DACDMM010000000
VERSION     DACDMM010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.319"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            191..445
                     /locus_tag="IWD16_002824"
     CDS             191..445
                     /locus_tag="IWD16_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9089789.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IWD16_002825"
     CDS             446..763
                     /locus_tag="IWD16_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9089790.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          377 a          167 c          218 g          312 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taat
//
LOCUS       DACDMM010000309         1070 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.320, whole genome shotgun sequence.
ACCESSION   DACDMM010000309 DACDMM010000000
VERSION     DACDMM010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.320"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            47..832
                     /locus_tag="IWD16_002826"
     CDS             47..832
                     /locus_tag="IWD16_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP9089791.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT          418 a          159 c          175 g          318 t
ORIGIN      
        1 aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc ctaacaaaaa
       61 tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat ccattatttt
      121 tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa aagaactttt
      181 ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac ctgatagtga
      241 taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat acatcaggga
      301 taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag atgctgcttt
      361 aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga atgactactt
      421 gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt tagaggaata
      481 tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg tatatcgagc
      541 tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa ataaatacaa
      601 tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa aatggaatcg
      661 tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt ggaaagcatt
      721 aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa atagttattt
      781 agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact aacaatatcc
      841 ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa aactttgaat
      901 ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac gaaacaatcc
      961 ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa cttaagaaaa
     1021 atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DACDMM010000310         1056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.321, whole genome shotgun sequence.
ACCESSION   DACDMM010000310 DACDMM010000000
VERSION     DACDMM010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.321"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(104..334)
                     /locus_tag="IWD16_002827"
     CDS             complement(104..334)
                     /locus_tag="IWD16_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089792.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(428..766)
                     /locus_tag="IWD16_002828"
     CDS             complement(428..766)
                     /locus_tag="IWD16_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089793.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
BASE COUNT          342 a          211 c          115 g          388 t
ORIGIN      
        1 tggatactct ttatactgca ttaaagttag taatattaac agcaaagata tcgaaaaaac
       61 aataacataa tatgtgtcaa aatttttcat aataagaact cccttactgt tgcatactaa
      121 cataatccaa aaactcttct acttcagcta aagacattcc actttcttta gtccaaaaaa
      181 cagttttttc tatcgatcct tctctgtaaa ctgtaaatgt cggagtcgag ttaattgagt
      241 agcgactctt gaattctttc cactcatttt catcttctct aacttttgct acattcacgt
      301 aataaatatt ttcttttccc ttttctacca gcatatcgaa tagcttcctg cttttgatga
      361 tcgatttctt gtgctacatc tagtaaattt tcatcaattt ctatatcata aagtatttca
      421 ctagtgtcta catccacaat atatactttt tatcagtagc aatttttgag taatccgtta
      481 aaatgatctc agtctcgtca ctttcatcag tataaatata cgttaacaat gaactcacct
      541 tttcaatcgg tgttagctct aagcctactg tattttttac tattgagtca ttatttacca
      601 ccataacttc gtactgtata taccatccta tttggtttcc atcaatatca gtaattggct
      661 cactagctaa tggagtctcc ggaacaatat tttcctgata tttcttaaac tcttctgtag
      721 cacgaaaaag gttatcatgt tcctcacttg aaactggttg agtcatagaa aatgttactt
      781 ggctaaacag agggaaaata aacactacaa tcaacaaagt ataaaaaaaa gcccttcttt
      841 ttcatttttt atcttccaat cacaaattga aaaacttatt cctgcaactt tgagtctagt
      901 atcacaatcg actgactaca actattcatt tacattctac tttaagttcc tccttaacca
      961 ttaattttat taaagatact tgtaatagtg attctcacaa aatttcatga aaaatacttt
     1021 attactgtat ctaaaattcc aatacacaac ttatcg
//
LOCUS       DACDMM010000311         1053 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.322, whole genome shotgun sequence.
ACCESSION   DACDMM010000311 DACDMM010000000
VERSION     DACDMM010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.322"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..479)
                     /locus_tag="IWD16_002829"
     CDS             complement(<1..479)
                     /locus_tag="IWD16_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9089794.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIG"
     gene            complement(589..>1053)
                     /locus_tag="IWD16_002830"
     CDS             complement(589..>1053)
                     /locus_tag="IWD16_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP9089795.1"
                     /translation="SISPGIFGLLSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGG
                     AYLPWFISSISMAILADIILSVFGYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFR
                     QDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT          385 a          205 c          150 g          313 t
ORIGIN      
        1 aatccaattt taaagttaat gaaaaagcct aaggcaactg ataaaacagg tgttactatg
       61 gcttgagcac tatctggaat caagtgagca attgccatat gtgttttagc cgcgttgtca
      121 tcaattgtct ttcgaatata tcccgaagaa tttaaatcaa aaaaacgaaa acttgcttct
      181 gttaaaccgc taattccctt ttttctcaaa ttagtctcta agcgaaaccc aaggatatga
      241 gaaaataagc cagaaaaaat ataaaataaa gctcctaaag ttaaccaagc aacagtttga
      301 accgcataac tcttagctaa atattcatct cctacaataa ttagagactg aagaaatttg
      361 taaatactgt aatatccata gactgttaat acgacagaaa aatcagaaaa taaagtggct
      421 aaccatccgt agtatttcat tttagggaca taactaaata attttttgta tactttcatt
      481 ttttcctccc ccgcattcaa tttcttctct tattcgttta caactgttaa tgctttccgt
      541 aatagcgtaa attagagatg tacaaacaga taaaaggtaa actacgttct attttttgta
      601 tttttttaag acttttcttc caatcaatac accaatcata gataaacttg ctacaactaa
      661 cactgaaatg attccccata tacctaccgc atagtggatc attgcatcca ttgttgcagc
      721 agattgtcct ttatctatcc aatcttgacg aaaacgatct gtaaagaacc atataggaat
      781 ccactgtccg gcagcactgc ctaattgcat tagagcccaa gacgcaactt gaggaattgc
      841 tcgatcatat ccaaatacag atagaataat atcagctaag atcgccatac taatcgaact
      901 gataaaccaa ggtaagtacg ccccgcccat taacgtaaaa aataacaata aaacaattgt
      961 gtaaatagta aaaattcctt ttctagggta gttatagcta atataaacaa atatgattcc
     1021 agataataat ccaaaaatcc cgggggatat gct
//
LOCUS       DACDMM010000312         1024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.324, whole genome shotgun sequence.
ACCESSION   DACDMM010000312 DACDMM010000000
VERSION     DACDMM010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.324"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..626)
                     /locus_tag="IWD16_002831"
     CDS             complement(<1..626)
                     /locus_tag="IWD16_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9089796.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
     gene            914..>1024
                     /locus_tag="IWD16_002832"
     CDS             914..>1024
                     /locus_tag="IWD16_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9089797.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREAL"
BASE COUNT          326 a          175 c          201 g          322 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga
      781 tttcaaggtg tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt
      841 caccaacact agatattata gagccataat atgctgtata atatctagtg tataggctta
      901 agtggaggta tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat
      961 aagtgaatat ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc
     1021 cttg
//
LOCUS       DACDMM010000313         1023 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.325, whole genome shotgun sequence.
ACCESSION   DACDMM010000313 DACDMM010000000
VERSION     DACDMM010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.325"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            178..987
                     /locus_tag="IWD16_002833"
     CDS             178..987
                     /locus_tag="IWD16_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9089798.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT          340 a          179 c          198 g          306 t
ORIGIN      
        1 tcaacgtttt tttaacaaaa aaagcgaaaa taatacttaa cgttcgtgtt tagcttatgt
       61 ttagctattt atatagccat aactgtagtt tttgttagga atagctgatt gctcttgtta
      121 attacctaaa atatgctaga ctaaaacaaa tgctagttga ggaaggaagg acccttattg
      181 aataaaaaat taattgccat cgatctggac ggaactaccc tcaatcaaga ttcgatgatt
      241 acatctaaaa caaaagagac tctaaaaaaa gcaatcaatg ccggtcatca tgtcagtatt
      301 gctacaggac gcccattccg aatgagccat cagttttacc aacaattaga attaacaaca
      361 ccgatggtta attttaacgg cgcattagta cacatcccta atcagcactg ggatggtgaa
      421 agagaaacgt tgatcaatcg tgaaatcgct tttgaaatct tatcgcaaaa aaagcagcta
      481 aatttagatt ttatcgctgc tgaaaatcgt gatacttttt ttattgacag ttttgatttc
      541 tttgatgaaa aaatttttgc ttcttctcgt ccaggagaga agaatttgct ttctcctaaa
      601 aatttgacga caaatcctac atctttgctt gtccgaacag acaaacgttt tgcaggagct
      661 gtatctgctg aattaacacg tcaatttggc agttatgtcg atgttcggac atggggtgga
      721 cctactgcca tcttagagat tgtttcaaaa ggaatccaaa aagcaaaagg cgtgcaagag
      781 attgctaact acctgtctat cgatcaaaaa gacgtcattg cctttggtga cgagcataat
      841 gatttggaat tgttggatta tgcaggttgg ggagtagcta tggctaacgg tactgaccaa
      901 ctcaaaggca ttgcaaatga tgtcactcct ttgagcaatc aggaagacgg tttagctgtt
      961 tatctggaaa aactacttaa actataatag agaattagat tccacaaaaa agaggttgtg
     1021 aca
//
LOCUS       DACDMM010000314          999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.326, whole genome shotgun sequence.
ACCESSION   DACDMM010000314 DACDMM010000000
VERSION     DACDMM010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.326"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            87..356
                     /locus_tag="IWD16_002834"
     CDS             87..356
                     /locus_tag="IWD16_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9089799.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            349..606
                     /locus_tag="IWD16_002835"
     CDS             349..606
                     /locus_tag="IWD16_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9089800.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          387 a          108 c          180 g          324 t
ORIGIN      
        1 tataagaagt tagaatatta atcatttgct taattgtaca atataatgta caattgtttt
       61 atagaaataa ataaggggtg aaaggaatgg aagcagtagc ttattcaaat ttccgccaaa
      121 atttacgtag ttatatgaaa caagttaatg aggatgctga aacacttatt gtaacaagta
      181 aagatgtaga agatacagtt gttgtattat caaaaagaga ttatgattct atgcaagaaa
      241 cgttgagaac actttctaat aattacgtca tggaaaaaat tcgtcgagga gatgaacaat
      301 tctccaaagg tgcatttaaa acacatgact taatcgaggt tgaatctgat gattaaggct
      361 tggtctgatg atgcttggga tgattatctt tattggcatg agcaaggaaa caaaagcaat
      421 ataaaaaaga ttaacaagtt aataaaagat atcgatcgtt ccccctttgc tggattagga
      481 aaacctgagc cattaaagca tgatttatct ggaaaatggt ccagaagaat tacagatgaa
      541 catagactga tatatagagt tgaaaatgaa acgatattta tttattctgc aaaagatcac
      601 tattaaccaa tcggaagtaa ggaaagggtc agaaacttaa aagtttttga tccttatttt
      661 atttacccta gtcatttaaa aagctaatat agcttagtgt tgattgttat taatgaatgt
      721 gtttgttacg cgtattacgg atataaggtt agtaaaatca tttctaaagt tgaggaaaag
      781 taaatataaa tggcttaaat ttcaacaatt tgaagttgaa tagatatgtt ataatactat
      841 tgtagtgtgg gatgttagtt actaaaggat gacgcttata tatatgactg aatagaataa
      901 gcaataggtt taataatcta ttttaaattt tttgtactag ttttagttaa ttagcaaaaa
      961 caacaaaaat aaacttctca tagaatttag ctaaaaatt
//
LOCUS       DACDMM010000315          998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.327, whole genome shotgun sequence.
ACCESSION   DACDMM010000315 DACDMM010000000
VERSION     DACDMM010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.327"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            55..411
                     /locus_tag="IWD16_002836"
     CDS             55..411
                     /locus_tag="IWD16_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9089801.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
     gene            401..667
                     /locus_tag="IWD16_002837"
     CDS             401..667
                     /locus_tag="IWD16_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9089802.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            826..>998
                     /locus_tag="IWD16_002838"
     CDS             826..>998
                     /locus_tag="IWD16_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP9089803.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYI"
BASE COUNT          415 a          129 c          194 g          260 t
ORIGIN      
        1 cgaattttta tgttatatac tataatataa aatcaatata tgatataatt gggtatggaa
       61 aaaatatctt ttgaaacatt taaaagacct aatggtcatg atgaattttt agaatggttg
      121 gaaacattac caaaaaaaga tagtgcgaag cttttacgta caatagaaga aacagaaaag
      181 aatgggatgc tagtagctca acggttgaaa tgggtgaaaa aattagacac taatttgtac
      241 gaattaagaa gtaaggtagg atcaaacatc caaagagcta tctatttcca tgtggtcaat
      301 ggaaggtatg tcattaccca tggatttact aaaaaaacgg agaaaacacc aataagtgaa
      361 cttaaacatg caaaagaact tagagaggag tggttcacaa atgatgatta aaaaattgat
      421 tgaagaaaag caaaaagaca aagattttgc agaagcatat gaccgtgaag gaagaaaact
      481 cgaaactgcg gttgctcttt atcatgcacg ggaggaagca ggcctaactc aagcggagct
      541 tgcacaaaaa gcacatacaa ctcaatctac tattgctcga attgagagag gggataacgt
      601 atctttcgat aaacttgatg atatcgctag ggcattaggc aaaaagctgg tagtagaatt
      661 tgcttaaaag cgagttgaaa atatacctgt aataggctcc taactaatca attagtttag
      721 tgggcctttt tgtactaaat agataaaagt tgtcctatag tgtaaaagtc aataaatttt
      781 atacaaaaat tactttaaag agacataaag agaaaggtac aataaatgaa aaccataaat
      841 ctaataaaag tatttatagc atcaccaggt gatatagcag caaagaggaa tgaagtagaa
      901 gaaaccatat atagatggaa tatagaaaat acagataata caaaagtaat tttgatgcca
      961 attcgatggg aaacgaatgc gattcctact tatataaa
//
LOCUS       DACDMM010000316          997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.328, whole genome shotgun sequence.
ACCESSION   DACDMM010000316 DACDMM010000000
VERSION     DACDMM010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.328"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          356 a          161 c          159 g          321 t
ORIGIN      
        1 ctgtctaact catatgtgaa tgatttataa aatatcccaa cgtctcatct aaagaaatgt
       61 tgatcgtcag caaggagcta aaaaatacga atatatttct tttgtagtaa aatcggactg
      121 gtttgcatat cattttcttc gtaaatatca aaatcatgtt aactactatc aattactaat
      181 aaaaactacc actttaagcg ttaagaactg ttaagtttag ttaagtgagg atagttcaca
      241 aaatccattg ttaaacttca cgaaagtggt aaaactcaaa atgctttata taggattaca
      301 gcatcgctct ttcttcgatt gcgcgttggg taaaaaatat tatgaagtaa aaattgacga
      361 tgacacgttc cttacggctc atcagatgaa acagctacaa aaacgaaatg atcaactcga
      421 agaaaattta attctaaaaa agcgattgcc atattcacgc ctcgctcgac gaactattaa
      481 aggtcaagat ttacctgaag atcatccttt ttaggaaatt gaagcttttt atgttgcaca
      541 atgcatttat aatactacaa tgatgttttc accagatatt cttctagggt gcggtgtaat
      601 gaaacagata cacttgcaaa agaaaattca ccgcgaattt actaaaatta tgaataatta
      661 tgttaatctt ccgaaatttt tttccaaaat taagggatca agctggtgtt atagattgtt
      721 taaggttggc aaaaaaataa cttcttttta aacaattata gaaaaccgat actctgttaa
      781 gttggttgac acggtgtcga tttttatgac tttcttattc tgaaacttca ctaacggaca
      841 gtacctaaaa agtctatagt atggacgttt acgagtacta ttttatcaaa aatgctgctc
      901 atttcaactt atatgtttta gtgaactacc cccacttagc ttggataggc tacaattaac
      961 tagacagaaa aattaaggtg tgtagactag aagaaaa
//
LOCUS       DACDMM010000317          997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.329, whole genome shotgun sequence.
ACCESSION   DACDMM010000317 DACDMM010000000
VERSION     DACDMM010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.329"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..913)
                     /locus_tag="IWD16_002839"
     CDS             complement(26..913)
                     /locus_tag="IWD16_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9089804.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DACDMM010000318          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.330, whole genome shotgun sequence.
ACCESSION   DACDMM010000318 DACDMM010000000
VERSION     DACDMM010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.330"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>994)
                     /locus_tag="IWD16_002840"
     rRNA            complement(<1..>994)
                     /locus_tag="IWD16_002840"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACDMM010000319          986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.331, whole genome shotgun sequence.
ACCESSION   DACDMM010000319 DACDMM010000000
VERSION     DACDMM010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.331"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..704
                     /locus_tag="IWD16_002841"
     CDS             <1..704
                     /locus_tag="IWD16_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9089805.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DACDMM010000320          959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.332, whole genome shotgun sequence.
ACCESSION   DACDMM010000320 DACDMM010000000
VERSION     DACDMM010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.332"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(107..511)
                     /locus_tag="IWD16_002842"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="IWD16_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="IWD16_002843"
     CDS             complement(570..>959)
                     /locus_tag="IWD16_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9089806.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DACDMM010000321          929 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.333, whole genome shotgun sequence.
ACCESSION   DACDMM010000321 DACDMM010000000
VERSION     DACDMM010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.333"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..338
                     /locus_tag="IWD16_002844"
     CDS             <1..338
                     /locus_tag="IWD16_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9089807.1"
                     /translation="VASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYI
                     HSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVL
                     RSIIDKSIE"
     gene            568..798
                     /locus_tag="IWD16_002845"
     CDS             568..798
                     /locus_tag="IWD16_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP9089808.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
BASE COUNT          318 a          139 c          146 g          326 t
ORIGIN      
        1 aagtcgcttc tttattaaca gttaatgatt tagtaatcat tttttcttta tctgggaaaa
       61 cgaaagatac ttatgattca ttgaaaatag ctaaaaaaaa tggcgcaaaa attattgcta
      121 taactaacta tattcattca cctataggaa agtctgcaga cttagtttta caaacagcca
      181 ttgaagaatt tcttaatggt ggatctttag ctggaaaaat atcacaactt tatatttgtg
      241 atctattagt tcatggttat gaacaaaaca ataatattga ttcagttgaa ttaagagaaa
      301 aagttttgcg ctctattatt gataaaagta ttgagtgaac tacccccact tagctaaacc
      361 taacggtttt taacgattga agtaggcaat tcttgggatg agagcatact tgatagctta
      421 ttttttacta atagcggtgg ctcttattga ccaaatatcc ctgtagttcc tacggttctt
      481 gattttatct aaagatattt ttatcgtgga agctattgat caacttgaaa gaaaaacgtg
      541 tagctcatta tcactagttt atcgagtatg gatgaagcaa tggatcatcc tctcttaaat
      601 aaatttatta aggatctgat ccttcaaatt ttttccatga tcgatgagca ggaacctact
      661 gaatctaaac gacatcgggc acaaggaatt attaagattg ctaagcgaat gttttctata
      721 aagggtgttc taagctatac agtgttaatt caaaagattc ccaatcatct tgtttataag
      781 aacaacgttg aagattaaaa aagtgtttca atgctaaaat tgcaaaatac ttcaatgtta
      841 tgagacaaac tgtctataag aatcaatttc cacccatttc atcacttgtt aaaagtttat
      901 gatccttttg atttttatat taatttatc
//
LOCUS       DACDMM010000322          925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.334, whole genome shotgun sequence.
ACCESSION   DACDMM010000322 DACDMM010000000
VERSION     DACDMM010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.334"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            292..925
                     /locus_tag="IWD16_002846"
                     /pseudo
     CDS             292..925
                     /locus_tag="IWD16_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          309 a          174 c          174 g          268 t
ORIGIN      
        1 atctcgaaaa tggttagatg taatcatcag gaaaaacaat cttaaacgaa ttactattca
       61 tggtcttaga cacacacatg ctagcttgct tttagcaagc tagtgcaaat attaaagatg
      121 tgcaagagcg tttaggccac tcttctattc aaatcattat ggatctatat attcatatta
      181 cagacaaaag aaaagaagaa accgtagcgc aattcgcaaa atataggctc tataatttat
      241 gggactgatg aatcaaaatt gattcttcag tcccattgtt attttaggta tatgattcgt
      301 acattgaaag agttgaaaaa ctatcttccg aagccgtcaa aaaggaccct gccaaaagta
      361 ttgatggtgg atgaatttcg ttcgcacgct tcaattgtgg ataaaatgag ctttatttgt
      421 gcggatggcg agactggaaa actcattgat attttgccta cacgaaaatt gtctcgatta
      481 acacactatt ttagtcgctg tgcaaatcct gaagcggtgg aatatttagt gacagatatg
      541 aacgccgcct acttccaact gacaaaacgt gtcttaccaa atgcgaaagt cgtcattgat
      601 tgttttcata tcatcaaaca tttgaatcag gcgttcaatg attttcgtgt acaagaaatg
      661 aaaagattac accagttagg gcaacgacgg gaagccgaga aagtgaaaaa gaactggcgc
      721 tttcttctaa aaaaccgtca aaaaatcaat cactatgatt acaaaacatg gcgcagtttt
      781 cgtgcaccta aatacccgct actcactgaa gcaatgatga ttgatcgatt gctggcattt
      841 tcaacgcctt taaaggacgc ctatactttt ttccatgagc tgaccgaagc ctttagagac
      901 aaggaatcag acttattttt tacct
//
LOCUS       DACDMM010000323          908 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.335, whole genome shotgun sequence.
ACCESSION   DACDMM010000323 DACDMM010000000
VERSION     DACDMM010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.335"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            191..577
                     /locus_tag="IWD16_002847"
     CDS             191..577
                     /locus_tag="IWD16_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9089809.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            671..862
                     /locus_tag="IWD16_002848"
     CDS             671..862
                     /locus_tag="IWD16_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9089810.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          335 a          140 c          142 g          291 t
ORIGIN      
        1 ttttcttttc taagtataaa gataactatc cattatcaac taaaaaaaca tttttgaaaa
       61 taaacatcag cagtcctatc aatgcatgtg agcttataga agttattcgc agcgcagcat
      121 aattgcatta gcttcttttc gtgttttttg cctcttttcc ctttaaatct taatggagga
      181 ggtgtgcaat atgtcagata acgacgaacg cgtaaatgcc cagttgattc gattctttga
      241 aaaggtcatt ttgaacaccg ctaatactta ttataaaaaa caagaagaaa ttcaaaaaca
      301 tgaacaactt gatcaactcc caccatattt gtttactaaa gatactataa aatttaaaca
      361 accgattaat gttttaaatg tgactctcct tgttgaagat ataaaattag caaaaatcct
      421 tcctttgttg aaagaacctg aacaaatttt tcttatcgaa aaatttatct ttgataaaac
      481 tgataaagaa attggaaagg ttctggggat tacaagacag ggtgcgacaa atttaaaaca
      541 tcgactttac aaaaaaattc aaaaaatgct ggtttaatat ctgcataata actaattttg
      601 tatgcttctt tgctcgttct cctccttccc aaagaaatag attataagtc catgggaaga
      661 aggtagattt atgaattctt taaaagaaga tttcatttta gcaaaggctg gaaatgaaga
      721 agctgttgag gcaattctca aaagatttag ctctttgatt cataagcaat cctggagaac
      781 tggaaaatat gatcaagatt gttatcaaga atgtatgctt gcaatatatc tagctatctc
      841 gaaattcgaa attaaagaat aactcaacta agggagtctg tgacataagt aaaaagatag
      901 tttcaaat
//
LOCUS       DACDMM010000324          902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.336, whole genome shotgun sequence.
ACCESSION   DACDMM010000324 DACDMM010000000
VERSION     DACDMM010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.336"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            263..886
                     /locus_tag="IWD16_002849"
     CDS             263..886
                     /locus_tag="IWD16_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9089811.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          332 a          129 c          167 g          274 t
ORIGIN      
        1 gttttaaaat aaactaatgt ccttattttt gtgttcacat ttctgttacc taagaaatac
       61 ttaaaggagg tgatgtagcc agcaacgact acaacttgaa cccgctatct acagtttatt
      121 tgttgtttct tttgggtata ctctaatggt acaaatccat atttgcttaa agcgttgatt
      181 tttcagcggt ttttctttat tgcaaagtgt accctaagac ctgtatacta atagtaaata
      241 gattggatag gaatgatggc aaatgtattt agcttattta agagtttctt ccgacgatca
      301 aagtttagct cggcaacata agctaattga aaattggtca gtcaaaaata atattgctga
      361 agacgattta aaaattttcg aggaaaaagt ctctggaaaa aatattgaaa acagaccaga
      421 gctgcaagag cttatttctt tcatacgtga acgagatacg attattattc ctagtcttga
      481 tcgattagca agaaatagta aagatacaaa agacttactt caattgtttc gtagtaaagg
      541 tgcttctatt gaaatattag acctaccgtc gtttaatggg gtaactgacc ctgcattaag
      601 agacttgcta acaaatttag tcatagaagt gtttagctat gttgctgaaa acgaacggaa
      661 aaaaatcaaa gagcgtcaaa aacaaggcat tgaaattgca aagaaaaatg gtcgttatac
      721 tggtaaaaag attcaatacc atgccaatgc aacaggaaaa aacaaattga tttacgatga
      781 aattgttgat ggtctgaaga aaaaagaaac tatttctaaa atagcaaaga acgcaggagt
      841 tacaagaaaa accgtttatc gagtaaaaag agatttagga atgtaatatg ttccagttag
      901 aa
//
LOCUS       DACDMM010000325          876 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.337, whole genome shotgun sequence.
ACCESSION   DACDMM010000325 DACDMM010000000
VERSION     DACDMM010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.337"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..236
                     /locus_tag="IWD16_002850"
     CDS             <1..236
                     /locus_tag="IWD16_002850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089812.1"
                     /translation="KSMKNEKSWHLAQKLLGDFSKKKVIPVFLMGIVFFSIEIYSLAI
                     LKNENFFVSVLMGESLILILILCLIVHEVNKQL"
     gene            660..>876
                     /locus_tag="IWD16_002851"
     CDS             660..>876
                     /locus_tag="IWD16_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089813.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHY"
BASE COUNT          344 a           89 c          158 g          285 t
ORIGIN      
        1 aaaagtcaat gaaaaatgaa aaaagttggc atttagcaca aaaattgttg ggggattttt
       61 caaaaaaaaa agttatccca gtatttttga tgggtattgt ttttttcagt atagagattt
      121 atagtttagc tattttaaaa aatgaaaatt ttttcgttag tgtattaatg ggtgaaagtt
      181 taatcctaat actgattttg tgtttaattg ttcatgaagt gaataaacaa ttgtgaatta
      241 aaaaaataaa aacctagtat aatcacttta ataaattgtt ggtagaaggt gatcaaaatg
      301 ggagtaaacg aaatgatgaa agtcagattg agtttttaaa tgataccttc ctcagaactt
      361 caaaacgaat tcaaagattc gttgacaaat cttgagcaag atcatttaaa aaaatatttt
      421 ctcttcattt gacgaaaaat gtttagaaat cttatataag tgaagataaa gtaattggtg
      481 taattaattg tagagaatag cctttgagca attgaagcga tacaattatt tagaaaatga
      541 acttcctcca gcatactaca tgattcctta cgggacgatg ttaaagaaga aagcaagaaa
      601 ttttagaagt attagtagat aaactaatgg gcttttctca atttaaggga gtagcccaaa
      661 tgtttatcaa atatggtgca gatacagtag caaccatgtt tacagaagaa tatcaagtag
      721 actattatga agtaatagat aaatgtgtac attttgttta tgaaaaagtg tggaatttta
      781 tgaattttgc gataagtgaa gaaatcttac gagtacaaga aaacagaaca ggaatattta
      841 ccagagacag aaagcttaat ttgattgcgc attata
//
LOCUS       DACDMM010000326          871 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.338, whole genome shotgun sequence.
ACCESSION   DACDMM010000326 DACDMM010000000
VERSION     DACDMM010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.338"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            157..771
                     /locus_tag="IWD16_002852"
     CDS             157..771
                     /locus_tag="IWD16_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9089814.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
BASE COUNT          312 a          147 c          160 g          252 t
ORIGIN      
        1 ttcataaaat atatttttaa tgtcataatc gtttaaattt tcttgattga catttattta
       61 caaaaaatat tgccatttaa aaagaaaagt gacatatggc ttcctcgtgt atgattaggg
      121 tgtattttaa atagttgcag aaagtaggta aaagttttga gagttgcata catacgtgtc
      181 tcatcgatcg atcaaaatga acaacgccag attgaagaaa tgaaaaaatt cggtgcagag
      241 agaattttca tcgagaaaca atctggcgca acgattactc atcgacctgt tttccaagaa
      301 gcattagatt acgtccgaga tcaagatatt tttattgtgg aagctattga tcgacttgga
      361 agaaattatg acgagatcat cgcttcagtc aattacttga agaagaaaaa tgtgaagctg
      421 atcattacta gtttaccgat catggctgaa gcaattggca atcctctcct ggataaattc
      481 attaaggatc tgatcatcca aattttggcc atgatcgctg aacaagaacg gactgaatcg
      541 aaacgtcgcc aggcacaagg aattaagatc gcaaaagcaa acggcgtcta caaagggcgg
      601 cccaaattat acagtgcaga cgcaaaagat ccccaacgtc gccttgttta caaaagcatc
      661 gttgaagatc ttaaaaatgg tgttgcgatt gctaagattg ctaaagacta caatgttacg
      721 agacaaaccg tgtatagaat aaaaaaagac agcatggtca atgacaaata gttgatcatg
      781 ctgcttttat tatcttctca tctttaattt cctacatata attactatct ataaaattaa
      841 gtcttactca gaacagataa agcataaaat t
//
LOCUS       DACDMM010000327          858 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.339, whole genome shotgun sequence.
ACCESSION   DACDMM010000327 DACDMM010000000
VERSION     DACDMM010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.339"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            101..>858
                     /locus_tag="IWD16_002853"
     CDS             101..>858
                     /locus_tag="IWD16_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9089815.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREK"
BASE COUNT          341 a          106 c          146 g          265 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaa
//
LOCUS       DACDMM010000328          858 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.340, whole genome shotgun sequence.
ACCESSION   DACDMM010000328 DACDMM010000000
VERSION     DACDMM010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.340"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            92..331
                     /locus_tag="IWD16_002854"
     CDS             92..331
                     /locus_tag="IWD16_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089816.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          273 a          158 c          131 g          296 t
ORIGIN      
        1 atactataaa agacttgata gttttgcatt gattttgcct aatactgagc ataggtgtat
       61 gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta tcagtttatc
      121 aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac cttcagatac
      181 gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt ctccggtaga
      241 aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac tgtcagcacg
      301 tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc aaataatgat
      361 gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta cgataacaag
      421 cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg atcggatgat
      481 ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag tataggtttt
      541 tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga accttttgaa
      601 taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa aagcttggtc
      661 ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg ttcatatttg
      721 ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt atattttgcc
      781 actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata ttcgtctacg
      841 cagatcacaa cacaattt
//
LOCUS       DACDMM010000329          843 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.341, whole genome shotgun sequence.
ACCESSION   DACDMM010000329 DACDMM010000000
VERSION     DACDMM010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.341"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            94..762
                     /locus_tag="IWD16_002855"
     CDS             94..762
                     /locus_tag="IWD16_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9089817.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
BASE COUNT          240 a          136 c          144 g          323 t
ORIGIN      
        1 aaataatttt ttttcatttt cctcttgaag taagagtata agtcagtata ataaagaagt
       61 tgctgaaaat tttcaaaaga aaagaggcta gaaatgaaaa aattaaattt atttacagaa
      121 ttgtgcggaa tcattttagg cgcaggaatc tacggattag ctgtgacagg aatcaatatt
      181 ccatctaagc tggcagatgg tggggttact ggtattgctt tgcttttgca taatcttttt
      241 ggattcgctc cttctgtcac ttcactgctt tttaatttac ctttattgct tatctcattg
      301 tttctttttg gaaaacacgc ttttctccgg acgatcgtcg gaacatttgc gctagttttc
      361 tttttacatt tatgggagag ccttcatata cattttgctg tagatagtct actgatcaat
      421 agtttgatga caggtattct ttctggcatt ggttgcggtc tcgtctttcg attcggcgga
      481 agcacaggcg gaacagatat catttatcaa gccattgaaa aatattttca tattaagatt
      541 ggcaaaagtc ttttcttgat tacttttggt attttagttg tctccttgct ttatttggat
      601 ctcacacatt ttctgtatac attacttagc tgttctattc tatcttatac attgaataaa
      661 gtgaagtatc tggaaattgg atctgttttg ccccaattgg aagcaaaaca gactacagaa
      721 acatttgaac cacttgatcc tcttgaacag ttagagcact gattcttatt tttcactact
      781 ttatttaatt tacagaaata tcattatgta aactaaaatg atattattca gtaaaatctt
      841 ctt
//
LOCUS       DACDMM010000330          823 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.342, whole genome shotgun sequence.
ACCESSION   DACDMM010000330 DACDMM010000000
VERSION     DACDMM010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.342"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            19..504
                     /locus_tag="IWD16_002856"
     CDS             19..504
                     /locus_tag="IWD16_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089818.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
BASE COUNT          244 a          147 c          131 g          301 t
ORIGIN      
        1 attttaggag gagctatcat gcataaaggg atttacgcag gcttgttggg aactgtaata
       61 cttttttctg cctgtggata taaaacaaag aattctgtta aagaatcttc agcagccatt
      121 tcaacaactg aggttggttc aactaaaagt acttctacta cttccattac taatacttct
      181 actggtaatg tagattcgca acatagccaa tcaagtattc agtcgtttag catttcttca
      241 acatctgaaa tgcaagaaag cactgtacct tctcttccat tagaaaactc tgacaaagca
      301 cttctatttc ttattgccca ccgttccgaa ttacaaagtg acgatattgt tattagcttt
      361 tatcaaaaaa ttgatcgaga ttatttgttc acagcttcca gcaaacaaat ccgttcccaa
      421 ggaggatctg gaagcgttgg tttttatcga gtgtcacccg aaggatcgat tacgatgaca
      481 gatgcaaatg gaacaccatt ttgattagat gatattatac ggatttacag tactagcaag
      541 tcattattgg tatcattttt tctgagaaag ttatacggag tataaataag catttgcatt
      601 aacctaataa ttcgttacag ctatttctct tatgaatata ctcacttctc tacttgctct
      661 cttgataaat ttttggtatc tacttcattt tcggtcaact gagttaatga aatttattgt
      721 cgggcggttt tctagattca atttttctat atttggatag aagaatttac ttttttacct
      781 tttgacatat tttcttctta ttattttaat gatccctttt tca
//
LOCUS       DACDMM010000331          811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.343, whole genome shotgun sequence.
ACCESSION   DACDMM010000331 DACDMM010000000
VERSION     DACDMM010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.343"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /gene="tnpB"
                     /locus_tag="IWD16_002857"
     CDS             <1..672
                     /gene="tnpB"
                     /locus_tag="IWD16_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9089819.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DACDMM010000332          811 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.344, whole genome shotgun sequence.
ACCESSION   DACDMM010000332 DACDMM010000000
VERSION     DACDMM010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.344"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>811
                     /locus_tag="IWD16_002858"
     CDS             <1..>811
                     /locus_tag="IWD16_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9089820.1"
                     /translation="EVVTVTPRNYKLNFRKVNDLDTHYAMIGIAAGIADYPLYYDATN
                     EKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWILGQCSTVGEAKKLLKNINLANI
                     NYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDNPVGVLTNNPSFDYQLFNLNNYR
                     VLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKLNSVSGDSESES
                     ISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYEDSQIT"
BASE COUNT          276 a          113 c          150 g          272 t
ORIGIN      
        1 atgaagtagt tactgttact ccaagaaatt ataagttgaa ttttcgaaag gtaaatgatt
       61 tggatactca ttatgcaatg attggtattg ccgctggtat agctgactac cctctttatt
      121 acgatgcgac aaatgaaaaa ggattgagta tggctgggct aaatttttct gggtatgctg
      181 attataaaga aatacaagaa gggaaagaca atgtatctcc ttttgaattt attccttgga
      241 ttttaggaca atgctcaaca gtaggagaag ctaaaaaatt gttaaaaaat atcaatttag
      301 caaatataaa ttatagtgac gaacttcctt tatccccttt acattggcta ttagctgata
      361 aagaaaaatc aattgtcatt gaaagtatga aagatggact tcatatatat gataaccctg
      421 tgggcgttct taccaataat ccttcatttg actatcaatt atttaattta aacaattatc
      481 gtgtcttatc gagtgaaact cctaaaaata atttttcaaa tcaaataagt ttgaatgcct
      541 atagccgcgg tatgggaggg ataggcttgc ctggagattt atcctcagta tctcgttttg
      601 ttaaagcgac ttttacgaag ctgaattctg tatctggaga ttcagagtca gaaagtatta
      661 gtcaattttt ccatatctta ggttcagtag aacaacaaaa aggtttgtgt gatgtaggtg
      721 atggaaaata tgaatataca atttattctt cttgttgcaa tgttgacaaa ggaatctatt
      781 attatcgaac atatgaagac agtcaaatta c
//
LOCUS       DACDMM010000333          808 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.345, whole genome shotgun sequence.
ACCESSION   DACDMM010000333 DACDMM010000000
VERSION     DACDMM010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.345"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..153
                     /locus_tag="IWD16_002859"
     CDS             <1..153
                     /locus_tag="IWD16_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9089821.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKV
                     LMGITA"
     gene            215..>808
                     /locus_tag="IWD16_002860"
     CDS             215..>808
                     /locus_tag="IWD16_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089822.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRC"
BASE COUNT          287 a          121 c          158 g          242 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcggcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgag aaccttgggt tttcaagtgc tttttggttc
      241 attaatctta accatagtgt ttttaaagtg gaaaacaagc aacaaaaaaa gtatgtggag
      301 ccttgtagga atagttctta ttatttttac tgtgactgca gcaacagaag caaattattc
      361 gcataaaatt cagtcgtata aaggtcgttt agttatgacc aaaaaagatg ttacacgtta
      421 tacatcctca agaactggag gcctatatga ctatgcacca ttagatgcat tgaaatataa
      481 agatcattta aagaaacatg aagtaaaagt tggtgatcgg tgggagaatg catctcataa
      541 agcatataat tccaaaatag tctatacagt agaagatgcc gatgagcaaa atatcacatt
      601 acctgtattc aattattggg gaacagttgt gaaagtaaat gggaaaaaga gcactataga
      661 aaatgacaat ggtttagtgt ctattaaagg tacggggaaa agaactatag ttgaggtttc
      721 atctacgtat ccaactacga tgattctatc tttattaatt agttcaataa cttttatatt
      781 cttatttaca cgttatttaa taagatgc
//
LOCUS       DACDMM010000334          808 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.346, whole genome shotgun sequence.
ACCESSION   DACDMM010000334 DACDMM010000000
VERSION     DACDMM010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.346"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>808
                     /locus_tag="IWD16_002861"
     rRNA            <1..>808
                     /locus_tag="IWD16_002861"
                     /product="23S ribosomal RNA"
BASE COUNT          247 a          159 c          237 g          165 t
ORIGIN      
        1 ttggggagct gtacgtaagc tatgatccag agatttccga atgggggaac ccaacatctt
       61 taataggatg ttacgattgt gtgaatacat agcacattcg aggtagacgc agagaactga
      121 aacatctaag tacctgcagg aagagaaaga aaattcgatt ccctgagtag cggcgagcga
      181 aacgggaaaa gcccaaacca gcaagcttgc ttgttggggt tgtaggactc caatatggta
      241 gttctttcag atagtcgaat gacttggaaa agtcagtcaa agagggtaaa aaccccgtag
      301 acgaaatgtg gaagacacct aggaggatcc tgagtacggc ggaacacgag aaattccgtc
      361 ggaatccggg aggaccatct cccaaggcta aatactccct agtgaccgat agtgaaccag
      421 taccgtgagg gaaaggtgaa aagcaccccg gaaggggagt gaaatagaac ctgaaaccgt
      481 gtgcctacaa caagtcaaag cccgttaatg ggtgatggcg tgccttttgt agaatgaacc
      541 ggcgagttac gattgcatgc gaggttaagt tgaagagacg gagccgcagc gaaagcgagt
      601 ctgaataggg cgtttgagta tgtagtcgta gacccgaaac catgtgatct acccatgtcc
      661 aggttgaagg tgcggtaaaa cgcactggag gaccgaaccc acgtacgttg aaaagtgcgg
      721 ggatgaggtg tgggtagcgg agaaattcca aacgaacttg gagatagctg gttctctccg
      781 aaatagcttt agggctagcc tcggaatt
//
LOCUS       DACDMM010000335          805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.347, whole genome shotgun sequence.
ACCESSION   DACDMM010000335 DACDMM010000000
VERSION     DACDMM010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.347"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..>805
                     /locus_tag="IWD16_002862"
     CDS             6..>805
                     /locus_tag="IWD16_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP9089823.1"
                     /translation="MLINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFNS
                     GNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKGI
                     TMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPLK
                     RQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVTD
                     GLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV"
BASE COUNT          274 a          117 c          149 g          265 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      721 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      781 tctgtagcga tttcaaatta gttta
//
LOCUS       DACDMM010000336          801 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.348, whole genome shotgun sequence.
ACCESSION   DACDMM010000336 DACDMM010000000
VERSION     DACDMM010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.348"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            290..>801
                     /locus_tag="IWD16_002863"
     CDS             290..>801
                     /locus_tag="IWD16_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9089824.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIP"
BASE COUNT          301 a           90 c          127 g          283 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc c
//
LOCUS       DACDMM010000337          800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.349, whole genome shotgun sequence.
ACCESSION   DACDMM010000337 DACDMM010000000
VERSION     DACDMM010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.349"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>800
                     /locus_tag="IWD16_002864"
     CDS             <1..>800
                     /locus_tag="IWD16_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9089825.1"
                     /translation="DDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYALK
                     ISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWVP
                     WFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVPI
                     TLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTTV
                     FTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQ"
BASE COUNT          264 a          100 c          134 g          302 t
ORIGIN      
        1 gatgataaat atgttggcag agtagtcggc ggaacactgt catttgtgtt gataggttta
       61 gtaatttatt taagaataat gataaagggt gctcattttt ttgataaaaa acagtggaga
      121 tacgctttaa aaatctcttt gccgctaatc ccacattcct tagcaaatat agttttagca
      181 caattcgata gaattatgat taattcttat ttaggtcctg ctgcgggggg aatatattcc
      241 tacattagta acataggtat tattctaagc gtaatatgga tgtctactaa taacgcctgg
      301 gttccatggt tctatggtga gatgagtaaa aataatgaaa aaaaagtgaa gaaagttagt
      361 aactattatt tagtagtctt tactttgatt acgatgattg taatggtagt ttcgattgat
      421 ttagcaaaat ttatggctcc gaaagactat tattctgggt tatctttagt ggttccgata
      481 accttaggct atttctttca gtttttatat agtttaccag ttaatgctga attttatgag
      541 aaaaaaacat cgtttattgc gattggtaca gtagcaagtg ctatagttaa tatactatta
      601 aacatgattt tcatacctag gttaggaata attgctgctg gatacacaac cgtatttacg
      661 tattttttac tttttatttt tcattataat atcgctaaaa aaattacgaa taaacaatta
      721 tttgacacaa aaaaaatatg tatcataata atttttgtat caattttttc tttgatactt
      781 catattttaa tcaatcaaat
//
LOCUS       DACDMM010000338          792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.350, whole genome shotgun sequence.
ACCESSION   DACDMM010000338 DACDMM010000000
VERSION     DACDMM010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.350"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..765)
                     /locus_tag="IWD16_002865"
     CDS             complement(<1..765)
                     /locus_tag="IWD16_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089826.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHIL"
BASE COUNT          317 a          121 c          130 g          224 t
ORIGIN      
        1 taaaatatgt gtcagcatat cactaggagc cataccactt aattcttttc ccagtgggac
       61 tttcaattct tgtgctctag attgttgaat aaaaggaatt aagaatcccg cacttaaaaa
      121 aaaggtgagg acggtagctt ttaaaagact taagagtcta gcgatttttt ggtcccaaaa
      181 ataaaaagaa agaaacaacg ttcctccaat aaatacagaa accatagcga cagaaagtaa
      241 atgagtatac actactagcg tcatgccaat agacaaccag taccattttt ggtaatctct
      301 aatataaatt tcgtaacacc ccattagtat caaaggcaaa aatgttaaag cgacagcttc
      361 tcccaatgaa gcacgatgga atatgtcaat ggcacgatag gcggaaaatg tataaataat
      421 cgaaaataac agcgaaatat aacgattgtt ttttatttgt ttcatagaaa aataagaaac
      481 gaccattgtt aaaaaagtga taaaaaaata gtagatatta taacttaaaa caagatttcc
      541 catcatttta taaaataaaa aagctggata aagagtaagc catggataaa aaatattcat
      601 cattgttcca tgatgatcga agttattgaa gtttactgga gaactccata cattatctag
      661 tcctatgatt cgagataaat gaaagtaagt atcctgattg acactatgat agattccctt
      721 agacatgaat agagggatga cgtaagcaaa acttagaaat aacataaaaa tgatatctat
      781 tatttggttt tt
//
LOCUS       DACDMM010000339          792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.351, whole genome shotgun sequence.
ACCESSION   DACDMM010000339 DACDMM010000000
VERSION     DACDMM010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.351"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            140..547
                     /locus_tag="IWD16_002866"
     CDS             140..547
                     /locus_tag="IWD16_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089827.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            561..>792
                     /locus_tag="IWD16_002867"
     CDS             561..>792
                     /locus_tag="IWD16_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089828.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKR"
BASE COUNT          303 a          104 c          137 g          248 t
ORIGIN      
        1 tacaaatata cttataaata tgcttacaaa attagctaat atttccgttg acaaagcttt
       61 gttactattg ttaaactaaa ttgactagca aaagtgaaac ataaatttat taaataaatg
      121 taaaaattag gagaaatcaa tgtcttatag agtacaatta ataatttcag aagatgtaga
      181 aagtcagcaa tttggcacta atgttattaa taaagtaatt aatccagctc tttctattaa
      241 tgcgccttta attcctactg ctttatcatt ttctgtaaca gctgtggtgt ctgagataga
      301 ggatacagaa aagataaaaa tagtagaaat tgaagtttta aacaagaatg aaaaacaaat
      361 tttttctacg ggtgaagtat cggtaagctt gcctcctcaa gttaacgata taaactttaa
      421 tataaacgcc agaaatgtct tggttgaaga agcaggagag cattatgctg ttgttaaatt
      481 taatggaact gagattggaa ggcaaatctt tgatatcaag gtcaacaaac cagtggaaaa
      541 aaattaagga gacgatacgg atgcttgata tagtaccatc aaatacacat cggagtggta
      601 gcttaataaa ttttgctgcg atttttgctt gcgcatcggt aattctagca tctccagttg
      661 ttgtggatgc aagttctact ccgaaaacta aaaatgataa tcaacttgta attactacaa
      721 aaaatgagat taacacaatt tcaagtaatg atggcaaaat ttttgatgtg ataaatacgg
      781 ttttaaaaag aa
//
LOCUS       DACDMM010000340          784 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.352, whole genome shotgun sequence.
ACCESSION   DACDMM010000340 DACDMM010000000
VERSION     DACDMM010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.352"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            19..579
                     /locus_tag="IWD16_002868"
     CDS             19..579
                     /locus_tag="IWD16_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9089829.1"
                     /translation="MIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIHL
                     TPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFGK
                     PLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTTL
                     NTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            576..>784
                     /locus_tag="IWD16_002869"
     CDS             576..>784
                     /locus_tag="IWD16_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089830.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWL"
BASE COUNT          283 a           96 c          170 g          235 t
ORIGIN      
        1 tcagaggaag ttaagcaaat tattgatgct tgtcatacga aaagagataa attaatttta
       61 atgcttatgt atgagggtgg tttaagaatc ggtgaagtgt tatcgcttag gcttgaagat
      121 attgtcactt gggacaatca aatccattta acacctagag atgttaatgt taatgaagct
      181 tatattaaat taaggaagga aagaacaata catgtgagta aagaacttat gtcactttat
      241 acagattact tgatatatga gtatagtgag gaattggagc atgattatgt ttttatttcc
      301 ttaaaagaag gctattttgg gaaaccacta aagtaccaaa gtgttcttga tctagttaga
      361 agaatagtta aaaggactgg aatagaattt acatcacata tgcttcgcca cactcacgca
      421 acgcagctaa ttagggaagg atgggatgtt gcgttcgttc aaaagagatt aggtcacgca
      481 catgttcaga caacgttaaa tacctatgtt catctttcag atcaggatat gaaaaatgag
      541 tttaataaat acctcgagag aaaggagcat aagaaatgaa tgcttctagt aaaaggaaaa
      601 ttattagtca gagtgagatt agcaaaaaaa tagctgtaat gaatgaagaa atgcaggggt
      661 tttgggctaa taatagttgg gatataagaa aatgtccaca tccttctgcc atagaattaa
      721 gtaagaatcc tgctttaagg aatcgttggg ttcgttttga acgtgttaaa aatctgtggt
      781 taag
//
LOCUS       DACDMM010000341          783 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.353, whole genome shotgun sequence.
ACCESSION   DACDMM010000341 DACDMM010000000
VERSION     DACDMM010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.353"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..461
                     /locus_tag="IWD16_002870"
                     /pseudo
     CDS             1..461
                     /locus_tag="IWD16_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
BASE COUNT          285 a           87 c          149 g          262 t
ORIGIN      
        1 tttcaattga agaaagcatg agtcaattaa gagatagaat acttaaagag tgtgcaggtt
       61 cttctgagta taattattta gaaagtgacg aatttatagc attaagaaac tatgttgtta
      121 ataaaatatc accaattatt gattcgacag gtctatgttt attagatgaa ttcttggcgg
      181 atatcagtct atttataatg tttattgatg taattgaaga aacatcaaga aattttttgc
      241 tgtctaagtt cagaaaattt cgtatgaaaa atgataaaca aaaattattg gcaaatgaga
      301 tttggagtag tttagaagtc gaagaaagta aacaagaact tttgttgaaa tggatacttt
      361 ttcttgtaaa gaagttctat gtgaaaatac cagaaaccca ttgtctgata gttatgcacg
      421 gtaaaataac agcttctgcg atcgcaaggg aaacaaataa gttgctgaat acatatgttt
      481 atgaggcttt cgatatgcca attgaaggtg agactagtga cttaattaac ctaatcaaca
      541 atttttgtaa aagtatagat acgacgaatg gactcattct gctagttgat atgggatcat
      601 tggaacaaat gtatgaaaaa attgaatcca atgtaatagg tgatttggta attttaaaca
      661 atgtttctac agctttagcg attgaatgtg ggattcaagt ttgccaaaaa aagccaatta
      721 gtcattttta ccagatggat tttgattcgt ttcaagttaa agttcagtat tataaaggat
      781 tat
//
LOCUS       DACDMM010000342          768 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.354, whole genome shotgun sequence.
ACCESSION   DACDMM010000342 DACDMM010000000
VERSION     DACDMM010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.354"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            111..347
                     /locus_tag="IWD16_002871"
     CDS             111..347
                     /locus_tag="IWD16_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9089831.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            344..742
                     /locus_tag="IWD16_002872"
     CDS             344..742
                     /locus_tag="IWD16_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9089832.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT          284 a          117 c          134 g          233 t
ORIGIN      
        1 cattttttcg taagaaaaag caaaataaaa gtatatacat tgattaaaag tatatacttg
       61 gatataatgt acttactata gaatgagaat ggtatagagg gtggcgaaat atgaaaaaaa
      121 tgaaaaagta taaaattaga aaatctggca atagcgatgt aacaactatc ccaccagaag
      181 taaaagaatt catgggtgtg cagacaggtg acgcaatcag ttatgttttt caatctgatg
      241 gttctgtacg aatgatcaaa gcacaagaag aacctgatat tgattcatta gttgattcga
      301 tcatgaatca atatgaagac gcgttaaaag atttggttga tttatgaggt atctttcgac
      361 taaagaaatt atcaaaatca acgcgaaagt tattttacgt cactcgccag gcgaaatgat
      421 cggtgtaaaa gatgcaaatg cattagatat ggccgtcaaa cagccttcac aagcagtatt
      481 caatcaagaa ctctatcctg aaatctatga aaaagcggcg attttagcaa ttaatttagc
      541 taaaaaacat ccttttcata atggcaataa acgaactgct cttgttgcca tgttgttgtt
      601 tcttcaattg aataattgtc cagttgcttt ttctcgtcaa gaagctgttg attttatcat
      661 aatgattacc actagttcat tggatttcga ttcactaaag tccaagataa ccagttattt
      721 acgacaaacc gcaataaaat gaacgaatta aaaactttat ttctcaat
//
LOCUS       DACDMM010000343          755 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.355, whole genome shotgun sequence.
ACCESSION   DACDMM010000343 DACDMM010000000
VERSION     DACDMM010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.355"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            221..619
                     /gene="spxA"
                     /locus_tag="IWD16_002873"
     CDS             221..619
                     /gene="spxA"
                     /locus_tag="IWD16_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP9089833.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          266 a          108 c          144 g          237 t
ORIGIN      
        1 tttgaattag atttgaaaac tctaattttt aaatgataaa aaaagcgaga atgctggttt
       61 tactgtatat taaccttaga taactttcat ttttttaaaa aaattatgaa aaaaaggtag
      121 ctatttaaaa gttgattatg tataatagaa acagatggga gattattatg atcttctgtt
      181 acaaaaaagt gattcttatc acgaaaagga gtggtgcacg ttgttaacat tatatacttc
      241 cccaagttgt acttcctgca gaaaggctcg tgcgtggttg caagaacatc agattccatt
      301 tgttgaacgt aatatttttt cagaaccatt gaacagttct gaattaaaag cgattttaca
      361 aatgacagaa gacggaacag aagaaatcat ctcgacacgt tccaaagtat tccaaaaact
      421 gaacatggat ttagatgatc tgccgttaca agaattattg gaacttgtgc aaaacaatcc
      481 tggattgctt cgccgtccaa tcatgattga tgataaacgt ctgcaagttg gttttaatga
      541 agatgaaatt cgtcggttcc taccgcgcga tgtacgacaa ttagaattac gtcaagcaca
      601 attgatggct ggattataga aggataatac gaaacttgga aaagactggc tctttgtcaa
      661 atgatgttgg taaagagaaa tcttagaata atttggacag aacagaaaga agaaaacctc
      721 tttctgttct gtttatttgg ttaaaaatgt tttgc
//
LOCUS       DACDMM010000344          742 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.356, whole genome shotgun sequence.
ACCESSION   DACDMM010000344 DACDMM010000000
VERSION     DACDMM010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.356"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>742)
                     /locus_tag="IWD16_002874"
     CDS             complement(<1..>742)
                     /locus_tag="IWD16_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9089834.1"
                     /translation="EFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLML
                     LINYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELS
                     IRLYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRN
                     KQQAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFP
                     NQISFRQKQEIGFLFSTSKNLICRQVGQ"
BASE COUNT          248 a           91 c          135 g          268 t
ORIGIN      
        1 aattgaccga cttgtcgaca aataaggttt ttgcttgtgg aaaataaaaa accaatctcc
       61 tgtttttgtc gaaaagagat ttgatttgga aaaaaacaat agaaaaaata attaaagtag
      121 gctctctctg tttctgtctc ttcacaaggg cgactcactg aaaaagaagt aaagtattct
      181 gaataatcat gtgtatgctg caataagtcc atcaattcag tgatttcaga agaagttttt
      241 ggaaaatatg cctgttgttt gtttcgtaat atgaataaag ttaacagcaa gtagatattt
      301 ttttcttgtt ttgaaatttt ttctatttgc tcatctgtac tgttcatttt tagtgcattt
      361 aattcaagtg aggaaaatgg ccaatcgtta cctccaaatg tatctgaaag aaaaaagtaa
      421 agatagagac ggatagataa ctcatttcct tcaagagtac agcaattttt tacagatagg
      481 atccgtaaat ggaatttttt taaggaatga ttgagctgtt ttgtcagttt ttcgagatag
      541 gagggcgata tataaagttg ttcaagtaaa tatttttttg gtacagcagt ttgtggataa
      601 ttgattagta acatcaaata gttaaaacga ttggattctt ttaggtagca taattttaac
      661 ttagaaaaaa cagctaactt tttagtttga tcggtttctt tggtttggat ctctccttta
      721 tgattagaat aaataaattc at
//
LOCUS       DACDMM010000345          738 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.357, whole genome shotgun sequence.
ACCESSION   DACDMM010000345 DACDMM010000000
VERSION     DACDMM010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.357"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IWD16_002875"
     CDS             complement(<1..173)
                     /locus_tag="IWD16_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9089835.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISIT"
     gene            complement(192..347)
                     /locus_tag="IWD16_002876"
                     /pseudo
     CDS             complement(192..347)
                     /locus_tag="IWD16_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            423..738
                     /locus_tag="IWD16_002877"
                     /pseudo
     CDS             423..738
                     /locus_tag="IWD16_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          268 a          140 c          106 g          224 t
ORIGIN      
        1 gttattgata ttgaagaatt agaactttca tataaagcct tttctatttc attgcttgat
       61 ttcattattg ttaaacaaga atacaagcaa gaaagaacta tagttaatat gataaaaatt
      121 attaaagttc tatttttctt tcttgtaata tatgctatag cattttttat cactttccta
      181 actactcctc tctattttct tactccataa ctaattgcat ccactggaca agcattttta
      241 catcgtccac atctgataca ctccagatga ttgcaattct caactgaatc tttattcatt
      301 tggcaaactt ttttacattt tccacaatta atacacttat ccttatcctg aatcggctac
      361 actagactag acagaaaaaa taaggtgtgt agaataataa aaaacacact ggaggatttt
      421 tcatgccaag acgtcaacga agaacctatt caaaagaatt caaacaacaa atcgtcgatc
      481 tctatctcgc tggtaagcct cgcgcagaaa ttattcgaga gtatgagctt acgccttctt
      541 ctttcgataa atggatgaag caagcacaat caacgggctc attcaaagaa agagacaact
      601 taacaccaga acaagcagaa ttgatcgcac taagaaagaa aaataagcaa ctcgaaatgg
      661 agaatgatat tttaaagcaa gcggcgctga tattcggacg aaaagacaag taattgatgc
      721 caacaagcat aaatattc
//
LOCUS       DACDMM010000346          718 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.358, whole genome shotgun sequence.
ACCESSION   DACDMM010000346 DACDMM010000000
VERSION     DACDMM010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.358"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..637
                     /locus_tag="IWD16_002878"
     CDS             <1..637
                     /locus_tag="IWD16_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089836.1"
                     /translation="AIAVLNYLKKRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAG
                     SRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIV
                     KTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKP
                     VRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILKNVKIAESLDDIL"
BASE COUNT          283 a           86 c          121 g          228 t
ORIGIN      
        1 agctatcgca gtactcaatt acttaaaaaa aaggactcca ctatataaca aaagctataa
       61 tttttattgt ttttttccta atatatatac aggaagagag aatacaatac aaattccatt
      121 agttacagct gggtcaagaa ccgcattgca actttcggat atttttaacc ctatgataga
      181 tatttataaa gaaaaaaaat atatattttt tacgtctgtt tatgattttg agggcggaga
      241 acctgtcgga gaatatgaat tagtatgcaa aatagcagac ttagtaggaa aagataatct
      301 aattgttaag acgcatccta gagatgtaag aacaatttat aaagataatg gatttatcga
      361 ggataaaaat tcatcaatac cttgggaggc gattcaattg tcaggcgatt ttaacgataa
      421 aatatttatg acaattaatt caggaagtgt tatttcaggt agtacaatgt ctgcaaaacc
      481 tgtacgcacg tattatatgt ataaattgtg taacatagaa ggaaatccgt cttgcaaaaa
      541 gaatgcgtat gatattgagc gattgcttaa gcaaaaaacg atgaaaaaca ttttaaagaa
      601 tgtgaaaata gcagaaagct tagatgatat actatgagca aaactaaata tttaattaaa
      661 aatatgggaa tattaacaat aagtaatttt tcttcaaaaa ttttagtttt tttgttag
//
LOCUS       DACDMM010000347          704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.359, whole genome shotgun sequence.
ACCESSION   DACDMM010000347 DACDMM010000000
VERSION     DACDMM010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.359"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..360)
                     /locus_tag="IWD16_002879"
                     /pseudo
     CDS             complement(1..360)
                     /locus_tag="IWD16_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          203 a          130 c          146 g          225 t
ORIGIN      
        1 ttacacaacc gtaacggttg ttttacgaaa ttggacggga ctttgccagt ttaatttctg
       61 ttttatccgt tcattattgt agtaatagat atagctatcg atggctgatt ttaattcctc
      121 gaaacagtta taaactttcc cgtgaaaaat ctcttgtttt aataagccaa aaaaattctc
      181 cataagtgaa ttatctaagc agttcccttt ccgagacatg ctttgaaaga ttttgtactc
      241 cttcaatttg tttctataag ctttcatttg atacgaccaa ccttgatctg agtgaaacgt
      301 acgacgatag ggacaatcgt tagtggtctg gatagcttcc tccaaacctt ccattaccgc
      361 acgtgtttct gaatggtggt agcgaccgat ttgaacggct agggcggaaa tacggtgcaa
      421 atgcacggtg gtttcatgca atgcttacag ggcgtatttt gtaagggtat gtaacggaga
      481 taatcccgac ggtaatggat gtgagaggcc aagccgacgc tgacggtagg gcaaacaaac
      541 atagttgcac tctagcgatc gttatttatt tttaacggtc aaactagggt acaactatgc
      601 ttaaaacact tcgcctcaac ttctggtagc aaaagtgatt ttgaaagtca aaatattttt
      661 tatgagttat ttataattcg ccgtttgtat aattaagcga gaca
//
LOCUS       DACDMM010000348          699 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.360, whole genome shotgun sequence.
ACCESSION   DACDMM010000348 DACDMM010000000
VERSION     DACDMM010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.360"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>699
                     /locus_tag="IWD16_002880"
     CDS             <1..>699
                     /locus_tag="IWD16_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9089837.1"
                     /translation="IGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNY
                     SIPLIATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQIS
                     AIKEYGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFL
                     LIANVLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATV
                     ICSYVIYVVRKRAVG"
BASE COUNT          215 a           92 c          139 g          253 t
ORIGIN      
        1 aattggtttt ttcattgcaa atattttatc acagattatt tcagctactt atctgtcagt
       61 attagtgaga atatgggaat atatcgattt tgaggctaaa gatcctcagc taaagagaga
      121 aatgctaaat tactcgattc cattaattgc aactgttgtt ggatggtgga ttaatagtac
      181 agctgatagg tatgtggttt cctttatagt aggtgtctcg gcgaatggct tattgtctgt
      241 ggcatataaa atacctcaaa taattaatac tttgcaggga atatttatac aggcctggca
      301 aatttctgca attaaagaat atggagataa tgagactgca aaattttatg gaaatacatt
      361 tgttatgatt aatttgttaa tgtgcataac ctgttcaagt cttatcatat taactagacc
      421 gttttcacat attctttatg cgaaagactt ttatgaagcg tggaaatatg ttccattttt
      481 attaatagct aatgttttaa attgtgcatc aggtctgtta ggacctatct tatcagcaaa
      541 aaaagattct aaatccatga tgtggtctgc aatcataggg gcattagtta atgtgattat
      601 gagtgtagtc cttgtttata agataggggt tcaaggtgcc acaatagcca ctgttatatg
      661 ttcatatgta atatatgttg ttcgtaaacg agctgtggg
//
LOCUS       DACDMM010000349          689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.361, whole genome shotgun sequence.
ACCESSION   DACDMM010000349 DACDMM010000000
VERSION     DACDMM010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.361"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            132..344
                     /locus_tag="IWD16_002881"
     CDS             132..344
                     /locus_tag="IWD16_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089838.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>689
                     /locus_tag="IWD16_002882"
     CDS             506..>689
                     /locus_tag="IWD16_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089839.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFV"
BASE COUNT          245 a          103 c          121 g          220 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttc
//
LOCUS       DACDMM010000350          670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.362, whole genome shotgun sequence.
ACCESSION   DACDMM010000350 DACDMM010000000
VERSION     DACDMM010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.362"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..272
                     /locus_tag="IWD16_002883"
     CDS             <1..272
                     /locus_tag="IWD16_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089840.1"
                     /translation="KFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNKNYFKIP
                     AAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(308..>670)
                     /locus_tag="IWD16_002884"
     CDS             complement(308..>670)
                     /locus_tag="IWD16_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089841.1"
                     /translation="FNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDEYLK
                     ENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNETITK
                     TDMALMNREAEKIINKLT"
BASE COUNT          217 a          109 c           93 g          251 t
ORIGIN      
        1 ttaaattcga tcctacgaat agatttatca aagattttta taaagtatgg aattcagaag
       61 ttttcttttt agcaatcgaa gatagcttat taatcaatct ctactattct aataagaact
      121 atttcaaaat tcctgctgcg aaaactagaa tgaagaaggg tgtatacttt ttgtttgata
      181 tcgtgactga cgtgccggac gcacgtagcg atcatcggcg ttatgactat ataaagtata
      241 ctttcgttga tccagaaaga tacaaagatt aaataaaaaa ataacaaaca aactcgcttg
      301 aaattcatta ggtgagtttg tttattattt tttctgcttc tcgattcatc agcgccatat
      361 ctgttttcgt aatggtttca ttaggaatat agtcagcaca ttttcttttc tctaacattc
      421 tatacccact attatgtatt cctaaggtat tcaattcctt aactgttgaa aacaaattcc
      481 aaacggtttg gtggcttcct tttccgctac ttccattata aataatatca taattttctt
      541 tcaaatactc atcagatttt ttaaaagcac tgtagtatgc tcttgaaaca gcagttcttc
      601 tataagattc tttagaatta tatttatcat ctgattcaat ttgtttggaa atagataaat
      661 attcattaaa
//
LOCUS       DACDMM010000351          661 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.363, whole genome shotgun sequence.
ACCESSION   DACDMM010000351 DACDMM010000000
VERSION     DACDMM010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.363"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..320)
                     /locus_tag="IWD16_002885"
     CDS             complement(<1..320)
                     /locus_tag="IWD16_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAP9089842.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLT"
     gene            complement(323..>661)
                     /locus_tag="IWD16_002886"
     CDS             complement(323..>661)
                     /locus_tag="IWD16_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAP9089843.1"
                     /translation="EYSKETDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDE
                     IDEHRGELIKNLVTNRAKIQTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIK
                     NLVKSYLKMN"
BASE COUNT          187 a          131 c           83 g          260 t
ORIGIN      
        1 gtcagcttat caataagaga tccaaactca ctgtcaaaag tgtcctcctt attaatacaa
       61 gctctgagct ctttaacata attattgaaa tccctatttg gttgaatagt agcctctatt
      121 cctgtaatat cagaaaaaat ttcgcgcgag gaactacttc tctcagaata gagattttga
      181 tatgatttat tcattcttga cattgctgag tcaacctcaa cttggtaatc tttaagcttt
      241 atttcttgtt gaataatttc ttccaatcgc tcttcagttt ttgataaaga ttgatataaa
      301 gaatcaaggt tttgtaacat ttttaattca tcttgagata actcttcact aagtttttga
      361 tacctctttg atacctcttt gaaacctcat tatagttaac tttccaatct gatttattca
      421 gctctgctct ataactatca atttcatctg acatttcatt aattattgtt tgaatcttag
      481 cccgattggt cactaaattc ttaatcaatt ctccgcggtg ttcatcaatt tcgtctgtaa
      541 cttttatacc attgccaaag tcaattttgt caacactatc agttagattt ttgggaattt
      601 ccttaagtgc ctccacatca cgtttcagtt ccctataaat atctgtctct tttgaatact
      661 c
//
LOCUS       DACDMM010000352          658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.364, whole genome shotgun sequence.
ACCESSION   DACDMM010000352 DACDMM010000000
VERSION     DACDMM010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.364"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>658
                     /locus_tag="IWD16_002887"
     CDS             <1..>658
                     /locus_tag="IWD16_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9089844.1"
                     /translation="PTEEELCKIYGVSKITIRKAFKLLAESGTVERLRGKGTFVTQKK
                     ESLKLAGVHGFSDSLSTRGHKIRYTVLHSKIIKADEFLANKLQINVTDRVYNIKRLMW
                     EDGAPMGIDNFFASEEKFPTLFEKGSQYTSLYKLLEDDYDVKATSSIMEINGLSATPE
                     LSDLLQCIVGDPLFILEKVGKDKNNQPFHYSQSTVRCDRISYVIEVTENITLDTVHKN
                     D"
BASE COUNT          244 a           89 c          118 g          207 t
ORIGIN      
        1 cctacagaag aagaattatg taaaatttat ggagtaagta agattaccat tcgaaaagct
       61 tttaaacttt tagcagaaag tggaacagtt gaacgattaa gggggaaagg gacctttgta
      121 actcaaaaaa aagaaagttt aaaattagct ggagttcatg gatttagcga ttctctttct
      181 acccgaggac ataaaattag atatactgtt ttacattcta aaattataaa ggctgatgag
      241 tttttagcta ataaattaca aatcaatgta actgatagag tttacaatat taaacgttta
      301 atgtgggaag acggtgcccc gatgggaatt gataattttt tcgcatcaga agaaaaattt
      361 ccaactttat ttgaaaaagg tagccaatat acttcattat ataaacttct agaagatgac
      421 tatgatgtca aagctacgag ctctattatg gaaataaacg gtttatctgc aacacctgag
      481 ctttccgact tattgcaatg tatagtagga gatccgctat ttattttaga aaaggttggc
      541 aaagataaaa ataatcaacc atttcattat tctcaatcca cagttcgttg tgatagaatt
      601 agttatgtta ttgaagtcac agaaaatatc actttagata cggtgcataa aaatgatt
//
LOCUS       DACDMM010000353          644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.365, whole genome shotgun sequence.
ACCESSION   DACDMM010000353 DACDMM010000000
VERSION     DACDMM010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.365"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..378)
                     /locus_tag="IWD16_002888"
     CDS             complement(<1..378)
                     /locus_tag="IWD16_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9089845.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVA"
BASE COUNT          196 a          116 c           91 g          241 t
ORIGIN      
        1 agccacagaa taactcacgt gatgctgttt agcaaaattt tctactgaga tatatttttc
       61 ctgaaagaaa gccaatacca ttttcataga taaagaatcc gttatataac gagataacag
      121 agctttgcta gaacattttc cattaatttc cagacatata tccaattcac tgacggttac
      181 tcgcaaattt ttctctagat cgaacttttg taaatcatgt ttcagcttat cgatcgtttt
      241 tttcaagaga aatgtcgata gcgacaactc ttcttgaaca ttcgatattg actctgataa
      301 atttgagctt gcttctaaat atttaagtaa tgaataactt ctgtgatctt ctttgcttaa
      361 aaaaaactca taattcatta gtcatcagct ctttctacgt atattacttt ccatgcattc
      421 accaactagc tggggataaa actgatttcc tggatccgag tcactgttgt cagcgattct
      481 tgtttgtata atatacatcg tcctgtcttt tttttaacat tttggagagc gttttatttg
      541 gcctttctcg cgatcaaacc tgttattaga attatatcat ttactagtat gcatttttat
      601 taataaaatg gtatatttta tattgttata aaaagagccc aacc
//
LOCUS       DACDMM010000354          641 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.366, whole genome shotgun sequence.
ACCESSION   DACDMM010000354 DACDMM010000000
VERSION     DACDMM010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.366"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IWD16_002889"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IWD16_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP9089846.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..543)
                     /locus_tag="IWD16_002890"
     CDS             complement(349..543)
                     /locus_tag="IWD16_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089847.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          184 a          134 c          113 g          210 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatctcat ttaatttata
      541 catagaagac ctccagactt cttgatagct caagtatatc ttgaggtgca tttgttagta
      601 aggtactgtt ctatggggcg cttacgttaa aaatgttttg c
//
LOCUS       DACDMM010000355          630 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.367, whole genome shotgun sequence.
ACCESSION   DACDMM010000355 DACDMM010000000
VERSION     DACDMM010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.367"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..591
                     /locus_tag="IWD16_002891"
     CDS             <1..591
                     /locus_tag="IWD16_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089848.1"
                     /translation="VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDK
                     CIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLP
                     FEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETL
                     TNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
BASE COUNT          235 a           86 c           96 g          213 t
ORIGIN      
        1 gtctattgtc cggaattgtt actttatgat gtaaaaaata taaaaaaaat tcccaaaata
       61 gatgaaaaca gtaaattgat gaaaaacata caatcaatct ttgggataga agaagttttt
      121 gtcggagata aatgtatctt ttttgatcaa cctttattat ctgatggtta tggcatagat
      181 gaaacagata ttttcgctaa agtaaatagt atattttctg gaaaaaaaga aactttaatt
      241 aagcttcatc ctagagcaag gacaaatcgt tatggtgaaa aaaacatatt gaattccagt
      301 ttaccgtttg aagtcttgct acttgaaaat gacttctctt caacactttt gattagtcct
      361 atatctacaa tttctttttc accatctatg atgtttgaaa agacgatcaa ctctattatg
      421 ttggctaaat taattttaaa tgaatataca gcattatctg aagataaaaa agaaacattg
      481 acaaatattg ttcttttctg tgaacattat aaccgtctat cagaagaaaa aattttactc
      541 ccagaaaact gggcagaatt aagaaagttg gtagagtatg ggattattta acacactttc
      601 caagtcaaaa ttagttaagt ccagtttctg
//
LOCUS       DACDMM010000356          610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.369, whole genome shotgun sequence.
ACCESSION   DACDMM010000356 DACDMM010000000
VERSION     DACDMM010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.369"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..478)
                     /locus_tag="IWD16_002892"
     CDS             complement(<1..478)
                     /locus_tag="IWD16_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9089849.1"
                     /translation="MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSL
                     TLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIE
                     LDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQY"
BASE COUNT          217 a           82 c          102 g          209 t
ORIGIN      
        1 tgtactgata agtaataacg actttattta tacctggagg aatagttaat tcagttgtgc
       61 caaacttaga taattttgtt tgtactagtt tgccattaat agaagccact tgccctttat
      121 aacgataaat aggtgtcgtc aagacagtat ttttattatt cgaattatct aattcaattg
      181 taagtttgtt actcataaaa tgagttgttg gatttatttc ctggttacct agtaaatatt
      241 gatcattatt aatcatatca ggatgataga ttgactcttt gtttgcataa tcagtatgat
      301 tgtaattttc agctatagca acagcgtctt caggtgctat aagtgttaat gaatctttgt
      361 tagcatggat agaattgctt actcctgaga gatgcaatat tagtgcaaga aacgtaaaga
      421 ccattatttt ccatgaatta tttttagtag aaaaaatata atagatcact atactcatcg
      481 tataggctat aaataaagaa gaaaaaccat ttaaccgcca gacaaattgt agactcttaa
      541 ctggtgtatg gttaaaaagt tgccaaggaa ataaattggt tgaacaaaaa agaacaaata
      601 caccaagaaa
//
LOCUS       DACDMM010000357          579 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.370, whole genome shotgun sequence.
ACCESSION   DACDMM010000357 DACDMM010000000
VERSION     DACDMM010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.370"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..391
                     /locus_tag="IWD16_002893"
     CDS             <1..391
                     /locus_tag="IWD16_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089850.1"
                     /translation="FFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNL
                     SLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLT
                     IQEEKYKDSKIDKQIDDFPEEIIDRIE"
     gene            complement(445..>579)
                     /locus_tag="IWD16_002894"
     CDS             complement(445..>579)
                     /locus_tag="IWD16_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9089851.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          194 a           90 c          108 g          187 t
ORIGIN      
        1 attttttctt tttttcagtt atttattatt attgatcaat cacataccca ttcaactgat
       61 ttctgaacca gtaaagatca tcatcgattt ttcttatggt gctgcctata atcaatttat
      121 caagaaaaat ttaagcttgt acattaatct gaacacggaa gtgatagagg caggaaagcc
      181 tgagttcccg gatgttataa tcacgaatag gaataatcta tatgaagaaa gtgcatctca
      241 agtagtggtt tggctcgatc cgccaagagc agtagactgg gggaatctta caaagatttt
      301 gttgactatc caagaagaga aatacaaaga cagcaaaatt gacaagcaaa tagacgattt
      361 tcctgaagaa atcatcgaca ggatcgaata gtgaacttct tttagaaagt ccgactgctt
      421 tttctaaaaa tattatttgt caaattaagc gagacaaaac tttccattta cgtaactcaa
      481 aaaaacttct aatggtgttt ggtaatttag tgattttcta gggatatggt ttcttttgga
      541 cgcaacggat gagatgaatc cttgattgac ttggttgaa
//
LOCUS       DACDMM010000358          562 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.371, whole genome shotgun sequence.
ACCESSION   DACDMM010000358 DACDMM010000000
VERSION     DACDMM010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.371"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>562
                     /locus_tag="IWD16_002895"
     CDS             <1..>562
                     /locus_tag="IWD16_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAP9089852.1"
                     /translation="IQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVV
                     IISVNFEYSHIDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSE
                     REVVRDNAGLMAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMN
                     VQMRDELSELAKKVKVTFLTDPRWVR"
BASE COUNT          222 a           57 c          116 g          167 t
ORIGIN      
        1 aaatacagga ggaatatatg gaagaactga gaaaatttct ctcagacaaa aaaattctac
       61 tgattgcacc aggaaaaagt tctgtagatg aggtagaaag aataaaagag tttgttacca
      121 aagaagaagt cgtaataatt agtgtaaatt ttgaatattc tcatatagat atagattacg
      181 tatttttaag taatttaaga cgttttcatg aattaccgaa agaaagcaga actaaatgta
      241 ttgttacatc aaatatacaa gcatcggatg tatatttacg gacgagttat aaaaagttgt
      301 tatctgaaag ggaagttgta agagacaatg caggattaat ggcaattaga tttttagccg
      361 atatgaatgt ggatgaaata ttcttagctg gatttgatgg atattcacat aatgaagatg
      421 agaattatgg tgagcaatcc atggaaatta taacaaaatt tgcattatta gatgcaatga
      481 acgtgcaaat gagagatgaa ttgtcagaat tagctaaaaa agtaaaggtg acatttttaa
      541 cggatccacg ttgggttcga at
//
LOCUS       DACDMM010000359          554 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.372, whole genome shotgun sequence.
ACCESSION   DACDMM010000359 DACDMM010000000
VERSION     DACDMM010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.372"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(28..>554)
                     /locus_tag="IWD16_002896"
     CDS             complement(28..>554)
                     /locus_tag="IWD16_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9089853.1"
                     /translation="IPIYFYFLVLAVVFFELLGESSSSFSTLCFPEATSNHSSSFIQE
                     KKLREKIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQS
                     HLQQLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHE
                     LYMKKGYENKTKRM"
BASE COUNT          168 a           90 c           96 g          200 t
ORIGIN      
        1 gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct cataaccttt
       61 tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg tattgtgcgt
      121 tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat
      181 caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa ataactgctg
      241 caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca aatggatgag
      301 taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa ggtattgact
      361 gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc ttaatttctt
      421 ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa
      481 actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa gaaaataaaa
      541 ataaattggt attt
//
LOCUS       DACDMM010000360          548 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.373, whole genome shotgun sequence.
ACCESSION   DACDMM010000360 DACDMM010000000
VERSION     DACDMM010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.373"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            22..399
                     /locus_tag="IWD16_002897"
     CDS             22..399
                     /locus_tag="IWD16_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089854.1"
                     /translation="MGKIVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
BASE COUNT          175 a           71 c          120 g          182 t
ORIGIN      
        1 aaaaaaaggt gtgtgttcac tatgggaaaa atagtcgctg ttgcacagtc gctgcttgat
       61 atactcaagt ggctgatgga aggaagcaat tggatatttg tgttgccact attgcttatt
      121 agtctattta tcatgtttcg catggtcaga aaaaaagaaa cgtttctgat ttttatctgg
      181 tatgcagcat ccgttggggt tgctaccttg atggttgaat tggcgttagg tagttgggaa
      241 gcttgcgtat ttgctttatt ttttgctgta ctgtatctaa tcatttatat cccatatatg
      301 aaaagaaagt ctcggatagg cagaaaaggt agacatacaa ctactttttc tagtacagat
      361 tctctcgaac aaagtagaag aacgagaaga agacggtaat atttagtagg tatacgcaaa
      421 aaaagataaa ataatggata aactattgag atgagagatt tatttaattg gtggaaaaaa
      481 gcttttttca cttatataaa agaatctttt ttaaaggaat gcttgttgta tcgggataag
      541 ccattcct
//
LOCUS       DACDMM010000361          548 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.374, whole genome shotgun sequence.
ACCESSION   DACDMM010000361 DACDMM010000000
VERSION     DACDMM010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.374"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(285..>548)
                     /locus_tag="IWD16_002898"
     CDS             complement(285..>548)
                     /locus_tag="IWD16_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089855.1"
                     /translation="LAELSEGKTGLANILNFLKEIVHIDVSKISLMELTNTHIKEENV
                     PLFVFETEQKNQREELGEGYIWEEPGQMRSVLGTYEVEGVPFF"
BASE COUNT          181 a           99 c           62 g          206 t
ORIGIN      
        1 ttttaaattt tttagaaatg tgatataaca tccacttcag aatcaacgct gttaaattta
       61 tcatgtattt cttccttggt caactataaa ttaagcatta ctttgtgaaa tgatttttcg
      121 atcaattatt attattcgtt atacaaccgt tttcaacata ttttgaaaat aaaatgcact
      181 aaaaaactat ctttagcaga acatttcatt gtattttttg gaccaaacaa taaaaaaaga
      241 agtttgaaaa aaattcaaac ttctttgttc gcttaatctg tattttagaa gaaaggcact
      301 ccctctactt cgtaagttcc aagaacagag cgcatttgac ctggttcttc ccaaatatat
      361 ccttcgccta attcttctct ttgattcttt tgttcagttt caaagacaaa caacggaaca
      421 ttttcctctt taatatgtgt atttgtcaat tccatcaaac taatcttgct aacatcgata
      481 tgaactattt cttttaagaa attaagtata ttcgctaaac cagtctttcc ttcagaaagt
      541 tccgctaa
//
LOCUS       DACDMM010000362          546 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.375, whole genome shotgun sequence.
ACCESSION   DACDMM010000362 DACDMM010000000
VERSION     DACDMM010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.375"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>546)
                     /locus_tag="IWD16_002899"
     CDS             complement(<1..>546)
                     /locus_tag="IWD16_002899"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089856.1"
                     /translation="TQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQD
                     YAFTSKDLSDSEIGSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQ
                     KVAILNEQYEKMQIYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISP
                     AEFQVILFNSLYIKRGFGLGI"
BASE COUNT          167 a           96 c           68 g          215 t
ORIGIN      
        1 tgaattccta agccaaaacc tcgttttata tatagagaat tgaataaaat cacttggaat
       61 tcagctggag aaatttgtgt tcttaatgtc cctatatatt ttgtatacct cttcacatca
      121 aaatcattat aatcgtcata atactcattt aatgttttaa ctattcggtg aaacattttg
      181 aagtaccgtc caagatatat ttgcatcttt tcatattgtt cattcaatat agctactttc
      241 tgatcgtgta aacttgtagc aacgtctttt gtttttgttt gataattccc catcgacttg
      301 cttacatcaa atgtttcaaa ttttatatat tcaactgtgc ccttcagcgg tcctgattgt
      361 tcaattgtac tcccaatttc actatctgat aaatctttag aagtaaatgc atagtcttga
      421 ttaaaaccct tttcgttaaa aattgccaac ttctgtaaag caataacagc ttgtttggca
      481 ttttgatctt tttgtatttc aataaacata tttaataaaa aatagaactc acgatctatc
      541 tgtgtt
//
LOCUS       DACDMM010000363          544 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.376, whole genome shotgun sequence.
ACCESSION   DACDMM010000363 DACDMM010000000
VERSION     DACDMM010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.376"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            75..>544
                     /locus_tag="IWD16_002900"
     CDS             75..>544
                     /locus_tag="IWD16_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP9089857.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          195 a           99 c           85 g          165 t
ORIGIN      
        1 taaacgggaa aacaaatagc tagttttcag tttagcgctc tttttattat ataaaataga
       61 aaggagaaaa ctttatgtct atttataatt ttgaaactac tttagaagac ggcactgtct
      121 attcattgaa aaaatatgca ggcaaaccga taatcattgt taacactgca acaaaatgcg
      181 gtctagcacc tcaatttaaa caattagaag aactttacca agcttatcag acacaaggtc
      241 tggttatctt aggctttcct tccaatcaat ttaaacaaga gctagattca gcgactgaag
      301 cagcagaagc ttgtaaaacc acttatggcg ttaccttccc tatgcatcaa ttgattgacg
      361 taaatggaga aaatacagat cctcttttta cttatcttaa aaatgaagct cctggtactg
      421 tcactaaagc aataaaatgg aattttacta aatttttgat tgatagaaat ggcaaagtaa
      481 tcgaacgatt tgcaccacaa acttctccat tgaaaatgac cgctgcaatt gaatccgttc
      541 ttta
//
LOCUS       DACDMM010000364          521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.378, whole genome shotgun sequence.
ACCESSION   DACDMM010000364 DACDMM010000000
VERSION     DACDMM010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.378"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..513
                     /locus_tag="IWD16_002901"
     CDS             1..513
                     /locus_tag="IWD16_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089858.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          226 a           48 c           76 g          171 t
ORIGIN      
        1 atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc taacatactt
       61 ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact cagtatagaa
      121 caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag aagaaaaatt
      181 gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt tagcttttta
      241 agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga gattagtaaa
      301 cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga agaaaattcg
      361 gatagctttt tagaatttga aaaatatcta acagatctta tagataatag aaataatgaa
      421 ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg gaaaagtatt
      481 atttctcaaa atatggataa attaagatat taaggatgtt a
//
LOCUS       DACDMM010000365          521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.379, whole genome shotgun sequence.
ACCESSION   DACDMM010000365 DACDMM010000000
VERSION     DACDMM010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.379"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..445)
                     /locus_tag="IWD16_002902"
     CDS             complement(<1..445)
                     /locus_tag="IWD16_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9089859.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKG"
BASE COUNT          146 a           88 c           82 g          205 t
ORIGIN      
        1 tcccttttgg ttgtctatga agcttaattc ttactaatga cttcaattta ggaactttga
       61 tgaatttact atcaatcaaa gcaactgtac cattttgatt attcgttgta taactctgaa
      121 caggattttt cttacttttg aaacgtggaa atccaacgga tttatcccga aaaaaattct
      181 tgtatgcttt atctaaatta agttgggcat tggctaaagc aaggctatca atttctttca
      241 aaaacggaaa ttctttcttg tatttagctg gtgtcggaaa agtcattttt ttagaagaat
      301 catttttaac ttcttcatac gccttttttc ggtcatcaag cattagattg tagaccttac
      361 ggacacaacc aaaagatttg gcaaagaaga tttcttgttc ctctgttgga tagattctga
      421 atttgtatgc ttttagttgt tccataaagt tcacctactt tctatttatg attgtttcct
      481 tgattttgga tatacttctt aatcacatca attggtgcgc c
//
LOCUS       DACDMM010000366          510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.380, whole genome shotgun sequence.
ACCESSION   DACDMM010000366 DACDMM010000000
VERSION     DACDMM010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.380"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          179 a           70 c           83 g          178 t
ORIGIN      
        1 aagaaaatct taattaggca atgtgtagac aacgtgaaac gattaatcaa ttaaaagcta
       61 aatacgtttt gagaaatgat aacttgtagt cgatggaaga catgtagata aaaaaactac
      121 gtgtctttta tttattctaa ttttttcgaa aaaaagacac tctctgattg ttgtattaat
      181 ggaaaagggg aaagcagcaa gtaaatttag tttttctatt aaatattctt cactttttaa
      241 ccttttatag tacttagatt taatcctata tgtttagaga gttcattggc agcatgattt
      301 tgcaaagctt ctcgaattat tgttatatta gaaactttac tgttcataat tatgcttctt
      361 ttttaactca aagtccaaag ggctacagtg gactttgagt taaaaataaa aagccctagt
      421 tattttgaaa aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat
      481 ttacctgttc cgtgggtttg gcacccaatg
//
LOCUS       DACDMM010000367          499 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.381, whole genome shotgun sequence.
ACCESSION   DACDMM010000367 DACDMM010000000
VERSION     DACDMM010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.381"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..489)
                     /locus_tag="IWD16_002903"
     CDS             complement(<1..489)
                     /locus_tag="IWD16_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9089860.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSM"
BASE COUNT          174 a           82 c           52 g          191 t
ORIGIN      
        1 catagattta aaggcagaat aattatttct ataatcagta ccttttctcc aatcaggaag
       61 ataagtagaa caaatagctc ttacagaata tactttttta ctttgaatac tattacgaac
      121 aacttccatg atatttttat acctcagtgg agcagcaaca taaatatttt tgtaatcata
      181 attatattga gtaagatcaa tactctcaga aaaagcaggt ttttctaaaa atatgtaatc
      241 aactttttca acaattacat ttagtgtttc cagatgctta aaagtcggat ttgtaataaa
      301 agctatatca taatgtgtat ccaaatcgtc tctattttga atatacgtta tttcttcaaa
      361 ctcttttttt ccactgctac tataagctgt tatatcaaaa ttaatagaat tcttttttcc
      421 atagcttatt agatttttta gatgtctctt tccaattgac cctaacccaa aaaaaattac
      481 tttcatcatt aatttacct
//
LOCUS       DACDMM010000368          496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.382, whole genome shotgun sequence.
ACCESSION   DACDMM010000368 DACDMM010000000
VERSION     DACDMM010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.382"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>496
                     /gene="scm"
                     /locus_tag="IWD16_002904"
     CDS             <1..>496
                     /gene="scm"
                     /locus_tag="IWD16_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9089861.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRIT"
BASE COUNT          180 a          122 c           85 g          109 t
ORIGIN      
        1 acatcagctt caagtgacag tacagatact acctctacca caagtgatag cacggacacg
       61 tcagcttcaa gcgacagtac ggatacatct accacaacaa gtgatagcac ggacacgtca
      121 gcttcaagtg acagtacaga tactacctct accacaagtg atagctcaga ttcatctacc
      181 actacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac atcctctacc
      241 acaagtgata gctcagattc atcaacttca agcgatagta cagacacgtc gtctaccaca
      301 agcgatacac gtcatgcaag tgtctccttc tcaaaaacaa gttcggataa caggacgaac
      361 tttaacttaa ctaataaaac aagcaatgac aatggaaaca aatcaaaaag agcacttcct
      421 aaaactggaa gtcaatcgaa taattggata acactcgcag gcgttatatt acttgtaatc
      481 ggtttaagaa tcactt
//
LOCUS       DACDMM010000369          485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.383, whole genome shotgun sequence.
ACCESSION   DACDMM010000369 DACDMM010000000
VERSION     DACDMM010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.383"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(62..259)
                     /locus_tag="IWD16_002905"
     CDS             complement(62..259)
                     /locus_tag="IWD16_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9089862.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DACDMM010000370          479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.384, whole genome shotgun sequence.
ACCESSION   DACDMM010000370 DACDMM010000000
VERSION     DACDMM010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.384"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            32..479
                     /locus_tag="IWD16_002906"
                     /pseudo
     CDS             32..479
                     /locus_tag="IWD16_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT          170 a           75 c          101 g          133 t
ORIGIN      
        1 tactttttaa gaaagtaggg tatacaagcg tatgaataaa gacataactt tccctgtgat
       61 ggacgttcgg cttgttccaa ttaccaaaat acaggcgaac gattataatc ccaatcgagt
      121 agcgtctccg gaaatgaact tattggagct ttctatcatt gaagatggct ttactcaacc
      181 attagtttgt tattacaagg aaaaagaaga gaagtatatc ttggttgatg tgcttcatag
      241 gtatcgtgtt gctaaggaaa gactcaactt aaaagttgta ccggtaacag ttattgataa
      301 acccatataa gaacgtatgg cttcaacaat tcgacacaat cgtgcaagag ggacccatga
      361 aataagagga atggaaattg tcatccaaag attagtaaaa aatggatggt cagacaagag
      421 aatatccaaa gaattaggca tggacattga agaaattttt cgctttaagc aagtgtctg
//
LOCUS       DACDMM010000371          461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.385, whole genome shotgun sequence.
ACCESSION   DACDMM010000371 DACDMM010000000
VERSION     DACDMM010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.385"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>461)
                     /locus_tag="IWD16_002907"
     CDS             complement(<1..>461)
                     /locus_tag="IWD16_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9089863.1"
                     /translation="SIGATLAGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFS
                     VCAVICYVICYKLTVERVKSDESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLL
                     TMSMNNYVFPDYYNNAQGIVLMNFINPILMLVLVSPVSLIFSKKYGKKELAS"
BASE COUNT          171 a           90 c           56 g          144 t
ORIGIN      
        1 gacgccaatt cttttttacc gtatttttta ctgaaaatta aacttacagg tgaaacaagt
       61 accaacatca aaataggatt gataaagttc attaaaacta ttccctgtgc attattataa
      121 taatctggaa atacataatt gttcatactc attgttaaaa gctgagcaag caacaaaaaa
      181 atagctgcag ctattataga aagcaacgct ctatttttag ctaatccttt taaagatttc
      241 aacaatgaaa cttttaactg cgattcatca cttttaaccc gttctacagt taatttataa
      301 catattacgt aacaaataac tgcacacact gaaaatactc ctgccacaat tgtaaaagtt
      361 gatccaccac gtacaatttg agctccagaa ctatctcttg tataaataaa aattggtgtt
      421 aacacgccaa tgaacatacc tgctaaggta gctcctatac t
//
LOCUS       DACDMM010000372          458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.386, whole genome shotgun sequence.
ACCESSION   DACDMM010000372 DACDMM010000000
VERSION     DACDMM010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.386"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            178..381
                     /locus_tag="IWD16_002908"
     CDS             178..381
                     /locus_tag="IWD16_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9089864.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
BASE COUNT          178 a           52 c           82 g          146 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttacact ctcgtcaatc
       61 attgttatat aataaaatgt tatataataa aattactaat gatatttaat attacaagca
      121 atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa gaaagaaaaa gaaagtattg
      181 aaacattgtg ttattctagg aatattagga acttgtctag ctggcattgg tacaggaata
      241 gacgttgacg cagctactta ctatggaaat ggtctttatt gtaacaaaga aaaatgttgg
      301 gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt ggggagataa tctttttggt
      361 agttttagtg gaggacgata atgacttcaa taaacattga tcacacaaaa aaagtcatac
      421 atgaattata caatagttta atgacacgac ctaatcag
//
LOCUS       DACDMM010000373          449 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.387, whole genome shotgun sequence.
ACCESSION   DACDMM010000373 DACDMM010000000
VERSION     DACDMM010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.387"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          188 a           62 c           75 g          124 t
ORIGIN      
        1 cattattatc aaaataatgt taaaaataaa aaagaattgt ccatcactgt tattcaaatc
       61 attttatcaa aactacatag taaatgttaa aaagcaacat gacataatat atagaaggct
      121 ttacagtata tatgggagct tactaaaaaa gaatggagta agagttaaca caatgggcca
      181 ttagctgaag ttttaagcac ttcaaggtat gaaacagaat ggcctgtaat actacatgat
      241 tatttagact gctcaaactg ctatcagtct ggtggaatga ataaagtatt atactaaaag
      301 cagatccaac tacaacgtaa caggaaacca gttgtatcag gaaaagtgat agtacatgga
      361 gaagaaaagg ccccctttgt atgagtcagt acatatctta tttatagata aaacggtatt
      421 caatcgaaat aaaaaatctg aataataga
//
LOCUS       DACDMM010000374          437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.389, whole genome shotgun sequence.
ACCESSION   DACDMM010000374 DACDMM010000000
VERSION     DACDMM010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.389"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          147 a           71 c           94 g          125 t
ORIGIN      
        1 ttgcaacaaa gaaatttcat cgggggaaga ggtttggaca aaaatggaaa tttccgccaa
       61 aagtaactgc tcctcaactg aaatcaatta aagaactaga atttgagaat gcactggtaa
      121 tttgtatgac atgtactgat gaagtatttc gaaaacattt ttaaaggttg agatgtatat
      181 agatttaacc aatcatttat ctctctcgga aaaggaatat taactaacca gcgggttatc
      241 aaaaataggt aggaaaactg tacttaatgg cttatttcgc gctgattatg agaatatcag
      301 tctgtttgat taatttttag ggttcaaaat acagagacaa actccaggtc gggtttcgcc
      361 acaagcgaat tacccgatct ggagttttgt ctattgcagg aaggcgtagc cggactgcag
      421 gaataaagcc cccggtt
//
LOCUS       DACDMM010000375          436 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.390, whole genome shotgun sequence.
ACCESSION   DACDMM010000375 DACDMM010000000
VERSION     DACDMM010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.390"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>436
                     /locus_tag="IWD16_002909"
     CDS             <1..>436
                     /locus_tag="IWD16_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9089865.1"
                     /translation="VSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPDASGE
                     IADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEKLYLV
                     ASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNV"
BASE COUNT          165 a           41 c          106 g          124 t
ORIGIN      
        1 ggtaagtgtg atagtaccaa tttacaatgt tgaaaaatat ttaagaaaat gtgtggatag
       61 tttattgagt cagacattaa aagagataga aatcgtatta gttgatgatg gttcgccaga
      121 tgcttcgggg gaaatagcag atgagtatca aaaaaaatat tctaatgtaa aaacaataca
      181 tagagaaaat ggggggttag gaccggcaag aaatacagga attgagaatg cgacaggaga
      241 atatgtggct tttcttgatt cagatgattg ggtagaaagt gatatgtatg aaaaattata
      301 cttagttgcg tccaaagaaa atgctgatat agtagttagt ggacattgtg attttgcgaa
      361 tgacaaagct atggtgaaaa agcaacatcc tttgagtggg aatgtatatg atacgcctaa
      421 tagtattaaa aatgta
//
LOCUS       DACDMM010000376          409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.391, whole genome shotgun sequence.
ACCESSION   DACDMM010000376 DACDMM010000000
VERSION     DACDMM010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.391"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>409
                     /locus_tag="IWD16_002910"
     CDS             <1..>409
                     /locus_tag="IWD16_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9089866.1"
                     /translation="VTHNKKISYQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFA
                     IELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAK
                     RNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDI"
BASE COUNT          142 a           53 c           76 g          138 t
ORIGIN      
        1 gtgacgcata ataaaaaaat ttcttatcaa taccatgtag gacagtattt acccgactgg
       61 catccttggg aagattatcg caattttttt gtttcaaaca aaaaaactaa tgggtgtaga
      121 gaaatattcg ctattgaatt accttggatt atagatacat ttgggaaagt taaatcgttt
      181 actgtaatga cccagaaatt atccaatctt gatattgatt attcggacag tttcactctt
      241 attattgagc atgaaactgg tatagttgga actttaagtg tagatgttgt ttcaagagta
      301 gctaaaagaa atttaactat aattggtgaa aagattttaa tagaatggga aggtaacccg
      361 gattctctat tttcatggaa tgaagaaact tctagtatga cagatattc
//
LOCUS       DACDMM010000377          409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.392, whole genome shotgun sequence.
ACCESSION   DACDMM010000377 DACDMM010000000
VERSION     DACDMM010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.392"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..379
                     /locus_tag="IWD16_002911"
     CDS             <1..379
                     /locus_tag="IWD16_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP9089867.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DACDMM010000378          382 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.denovo.394, whole genome shotgun sequence.
ACCESSION   DACDMM010000378 DACDMM010000000
VERSION     DACDMM010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.denovo.394"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>382)
                     /locus_tag="IWD16_002912"
     CDS             complement(<1..>382)
                     /locus_tag="IWD16_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP9089868.1"
                     /translation="GHKLGSPVDNYRSGTIAEIDYFYKNSKGEVGVFFLDEPVPQVLA
                     EEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKVNYLIEKAQNNLESKRFDLDSISY
                     KTPSVINKKVTGTDKDFDDNLDIFD"
BASE COUNT          118 a           73 c           47 g          144 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg cc
//
LOCUS       DACDMM010000379        12387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.guided.075, whole genome shotgun sequence.
ACCESSION   DACDMM010000379 DACDMM010000000
VERSION     DACDMM010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.guided.075"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..174
                     /locus_tag="IWD16_002913"
     CDS             <1..174
                     /locus_tag="IWD16_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9089869.1"
                     /translation="VSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLMEA
                     LAKGINEARNIEG"
     gene            complement(232..420)
                     /locus_tag="IWD16_002914"
     CDS             complement(232..420)
                     /locus_tag="IWD16_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9089870.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(518..1558)
                     /locus_tag="IWD16_002915"
     CDS             complement(518..1558)
                     /locus_tag="IWD16_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9089871.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1920..2417
                     /gene="dfrG"
                     /locus_tag="IWD16_002916"
     CDS             1920..2417
                     /gene="dfrG"
                     /locus_tag="IWD16_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9089872.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(2489..4441)
                     /locus_tag="IWD16_002917"
     CDS             complement(2489..4441)
                     /locus_tag="IWD16_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9089873.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(4448..4744)
                     /locus_tag="IWD16_002918"
     CDS             complement(4448..4744)
                     /locus_tag="IWD16_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089874.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(4919..5722)
                     /locus_tag="IWD16_002919"
     CDS             complement(4919..5722)
                     /locus_tag="IWD16_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9089875.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            complement(5738..5854)
                     /locus_tag="IWD16_002920"
     CDS             complement(5738..5854)
                     /locus_tag="IWD16_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9089876.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(6099..7028)
                     /locus_tag="IWD16_002921"
     CDS             complement(6099..7028)
                     /locus_tag="IWD16_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9089877.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            complement(7045..8067)
                     /locus_tag="IWD16_002922"
     CDS             complement(7045..8067)
                     /locus_tag="IWD16_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9089878.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            complement(8064..10091)
                     /locus_tag="IWD16_002923"
     CDS             complement(8064..10091)
                     /locus_tag="IWD16_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9089879.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            complement(10088..>12387)
                     /locus_tag="IWD16_002924"
     CDS             complement(10088..>12387)
                     /locus_tag="IWD16_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9089880.1"
                     /translation="NRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQT
                     EALISMIGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFV
                     SMPMAEIERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLP
                     KRYEDKKVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNS
                     EIFYYQQQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVV
                     DALESVDELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRP
                     FGDMLGLHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGR
                     NIYLQPSLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKA
                     ERGEWKETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGIS
                     SRDSEKFPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHL
                     LFSDGSVTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDF
                     IHSDRSIFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKM
                     KNNIGLKFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVM
                     QFHPIFEELLHAFDTRPPVRNEVEE"
BASE COUNT         3706 a         2391 c         2088 g         4202 t
ORIGIN      
        1 gtatcattac tccatgtatc tattgatgaa tttttcttac ctacagataa tttgattaaa
       61 agcactcgaa ggttacaggt agaaaaatac atggatagct ttacagacaa agaactatcc
      121 ttaatggaag cactagcaaa aggtatcaat gaagcaagaa atattgaagg ctagtcagta
      181 aaattcagat aaacaaaaag agccgataag atgagagttt catttctcaa attatcggct
      241 ctgcgtcttg gcgtctggct ctttgattgt tattatattc attttttgaa cattaattaa
      301 agtttcgttc ttacatttgg ggcagtataa agggaaattt ttaagtatag tatccactcg
      361 tattcgtagt cttgttttat ttccacaaat aggacacaat atccacttgt agtttataat
      421 aacaatctcc tcctttccac tttaattcaa atctatatta aagaatattt catcttattt
      481 aataagaaac catatttata taacaacata aaacgcacta agttatttta ttgaacatat
      541 atcttacttt atctatccga ctatttagac gacgggtctg gcaaacaggt tcgccagtgg
      601 taacctgata tccttttagc tctgctaaac aaacactaag cccatttgta aaaaaagtta
      661 aatcattgcg ataatcttga atacatcgag caggaatttc tccaataata atgacctcat
      721 tatttttcag ttgagtattt acgatatttg cacaatattt gggagcatcg ttatatgccc
      781 gtgaaagata ttcctgtggt gcataaactt taaaactaag atatggctct aacaattctg
      841 ttccagcttt tctaaaggct tgctccagta caataggagt aagcatccga aaatctgctg
      901 gagtactaac agggctatag tataaaccgt acttaaaaca gattttacaa tccgtcacat
      961 tccaaccata taatccttgt tcgcaaccat agcgtatccc ttccataact gcattttgaa
     1021 atgattgatt taagtatcca agagaaaccg agctctcata ctgcattcca cttcccaacg
     1081 gaagcggtga tacagataaa ccaatggaag cccagaaagg atttggcggc acttcgatgt
     1141 gaatggtata ttctgcattt tttaacggtc tctccatata aatgactgta ggctctttta
     1201 gttctatctc cacatgatac ttttcttgca acagtgcact aatcacttcc atttgtactt
     1261 tccctaagaa agaaagtata atttcatgtg tcgtagaatc cacgtaatat cgtagaagcg
     1321 gatcactatc tgagatttcc aaaagggcat caagcaacat ttctctctgt tcaggtttac
     1381 tcggttcaac agttgtttgt agtagagggt gcggattttc aatctttttt ctctgtggca
     1441 atagttttgt atctccaaga acactattta acttcaaaaa ctcattttgc aaaataacaa
     1501 tttctccaga ataagctcta tcaatcttac ataattcacc atttattgaa gtatacattt
     1561 ctgtaacttt tattttttct ttttctgata ctctaaccga atctcgtaaa tgtagtactc
     1621 cactataaag gcgtatatat gcaagtaagt agttcagttt tggagtacaa aatgacaaaa
     1681 tgtacttcaa aatggactac ttttttcatt ttaacttaca taccaaatta catacaaaat
     1741 gatttataaa atgcaattga aaatgataat tgaatgaaaa tttgtgattt tgtggtatta
     1801 tgataatata acttatagaa aaaattgctt attcaactaa cgggcaggat aatgagaaaa
     1861 ggttttttga tagaaataag aaattagatg gtaaattagt taaaaaggct gtgttataaa
     1921 tgaaagtttc tttgattgct gcgatggata agaatagagt gattggcaaa gagaatgaca
     1981 ttccttggag gattcccaag gactgggaat atgttaaaaa tactacaaag ggacatccga
     2041 taatattagg taggaagaac cttgaatcaa tcggaagagc cttacctgac agaagaaata
     2101 ttattctgac gagagataag gggtttacct ttaatggttg tgaaattgtt cattcaatag
     2161 aagatgtttt tgagttatgt aaaaacgaag aagaaatttt tattttcgga ggagaacaga
     2221 tttataattt gtttttccct tatgttgaga aaatgtacat cacaaaaata catcatgaat
     2281 tcgaaggaga tacttttttt ccagaagtga attatgagga atggaatgag gtatttgccc
     2341 aaaaagggat aaagaatgat aaaaatccgt ataactacta ttttcatgta tatgaaagaa
     2401 aaaacttatt gagttaacgg gtgcgttaat tgaagaagaa cagtaaagat aaagcacaag
     2461 agcttgggat tactccaagc tctttttgtc atttgaaata aattatatct ttccaatcaa
     2521 attgtttatc tgtaatccct aatctttgag caggtgtcaa aaccttttta tccgctgatt
     2581 taaaagggcg acaaaagttg tagtaagttc ttagaattgt aatcgcaaat tgagcatact
     2641 tcggattgaa gttagcgtaa atatagctct tcttatcacc acgagctgtt gttaatggtc
     2701 tttcaagaga tgatatataa cgtcttatct gttgaataaa gctattggtc gaatggtcat
     2761 taacatttaa tagcatcttt gcgatttcgt ttggttccag agcagataaa tccgttctac
     2821 aatcaactga ataaaatcct ttatccttag tggctaatgg atgtttgata gggttatcgg
     2881 catatttgcg atatctttct ccatctttac taatagcctc ttcgtggaat tgatgacttg
     2941 ttagtaattc agttagataa tgagatgcca aagtccgtag aaactttgtt ttgaatcctc
     3001 tgatatctcc ccaatcgagc agttctgctt ttgcttgtcc gaattctttt aagcattgtt
     3061 ttctgctttt cgtcttgtta acttggcaaa taaaatgatg tgcatcgctc aaccttaatt
     3121 ctttcgtgaa tactctataa aaggcgtttc taatagagaa gtcatcatca ctaatcattc
     3181 gccattcgtc ggcattaacc atttgtttaa ttaaccaata gtgagcagtt gtagtataag
     3241 gagcatcaac atttaaacca tcgacaaatc tactcctatt agtaattttc cctaattcgt
     3301 gaagataagc attcctattt tcgctatcgt tagcggatgg ttcttgtgga taataagacc
     3361 aatctaacct atcattttta cgactaaaag tattgagatg gtcttctttg aattttcttg
     3421 tatcttcatt taattcatcc attgaaatat tccaatcata ggcaacatca gaacggaaca
     3481 catatcttga taagacctca gcagatacaa ctatatacgt ttgcatatta aggtcttcaa
     3541 accccgtata tttctttgaa ccttgtcctt tcttacggac gttatttagg tagtagtgca
     3601 tcttgtccgt attaagccac atctcgttaa atttctttgt ttgaaggggt tgtgtctcat
     3661 atcgttctaa aaactccaaa caacgcctat acagccactc cagcttatga tagtaggttc
     3721 caactccaat tcctaaaata tcacaagtac gactgactga aacccttcca acaaccatct
     3781 tagcgaacat tggaagaatg gtgttctttt gttggtgata agtagtggtt tcttctcttt
     3841 ttggtagaac attagtgaac ttcttacacg ccttacattg atagcgttga gatttgcccc
     3901 tacttttccc ccttttgtaa aattgcttcg gctcattgaa tggggttgat tcttcttccg
     3961 agcagccttc tttatgaaat tgataatctg gctcaacatc ttggatgcta ttgatttcta
     4021 taaggcgttt aatttcttca acaacagacc aattagataa aggggttact gtacagtttt
     4081 gcgatacacc tctgccaata ggattgtcat tgcagtacat acctttatga cctttatctt
     4141 tggaactacc agtcatttta tatcgacttg gtttcccttt tacatctttg aatttgtgct
     4201 gttctttccc aaaattacaa caaaacggat tgatgcagta attatattga atgggatatt
     4261 cagaaccgtt ccatttaaaa gacgttggca agaacaacat tttgttatac cttgttgcaa
     4321 atcgttttgg agttaataaa tcatattgtt tagccctatc ttttaattct tcgtttgaaa
     4381 tcggaatttt aacatctaca atttcatctt ccttcgtggc taatctttta agcttggtca
     4441 ttgtctatca acccactcta tctttgatac ccattttttc aacaatccgc ttattacttc
     4501 gcttgttggc aatccattca ataaacttct tatcgtctaa aatgttcaat aagaattcat
     4561 ccactgcttc gctttcagta cgctctgcat attcagcgta ttgcttaact atcaccctta
     4621 cttgctctga tatttcccaa tccacagact tagcgttctt attttttggc tcaagaaatt
     4681 gcataggaaa atgcacccct ttttgcttag taaattgtaa ccattttaac acaccaattt
     4741 gcattcctac aataattatt tcacgttcgc atttttatat acattttaac ttacattttg
     4801 tggtgtaaat taacaacaaa cgttgattta acaataaaaa acgagtatca aattcagttg
     4861 caatttgata ctcatttagg tttacatttt cactttttgt actccaaaac tgaactactt
     4921 aatatgcaag acgttgtctt ttttttgtat attcaatttt gaaaacattt ccgcaaagtt
     4981 cagacggacc tcgatgtgtt gatgaataaa atttattagt aataacttct ataaggttat
     5041 caatccctat attacttttt gcacttccat gataaagagg gaacagagaa caattctgaa
     5101 atcttatgct ttcctcttgt tcgagttcca atgcttctaa tgatttaccg gacatatatt
     5161 tctctaaaag gtcatcgttt ccctctatta ccgtatccca ttgttcagat tcggtaaagt
     5221 tcgtcacaca catattagga tacagttcta ccttctgttt gattacaatt tcggcagaaa
     5281 gtttctcttt aatatcctga taaaccgttg ataaatcaat tccattttgg tcaatcttat
     5341 tgataaaaaa gattgtggga atccccattt tcctaagtgc atgaaataat atacgagttt
     5401 gtgcttgtac gccatctttt gcagaaatca gtagaattgc cccatctaaa actgataatg
     5461 aacgatatac ttctgctaag aaatccatat gtcctggcgt gtctatgatg ttcaccttcg
     5521 tattttccca ctgaaaagag gttattcctg tctgaattgt aattcctctc tgacgttcta
     5581 aaagcgtatt atccgtcctc gttgtacctt tgtccacgct tcctaattct gtaatcgctc
     5641 cactgttata taataagctt tctgttaagg tagtttttcc tgcatcaaca tgagctaaaa
     5701 ctccaatatt aataattttc atgtgatttt cctccattca aaaacccaaa agggcataaa
     5761 aatcccagtg ataaatactt ttatcactgg gatttttatg cataaccata ggtatacaaa
     5821 gcatacagat attctctgga tactttagaa tcacatgata aaggtattct taaactgggt
     5881 acaaaaaact aagccctcct aaaaaaggac atctaattat ttgttcccgc tatcaaattg
     5941 acagtttatt taagaatacc ttgccgcata tttattaact ccttttaaat agtcacttaa
     6001 ataatagcac gtaagagcat atttgtaaag gaatctccaa ttttttatca aaaagagtac
     6061 atgattacaa agtatctgta atcatgtacc aatatttgtt attttacaat cttccaatta
     6121 tcttgttttt ctaatatcaa ctcaaattgc gagatttggg ttgcctttgt ttcttgatct
     6181 aaatatttca cagcgacatt cacgattact tggttatctt ttctggtaaa gataggattc
     6241 accaattcct cgaaaacata ttccttgtta atcataggta acacatgatt acttacatag
     6301 taagtcaatt ctttttctgt ggcggtagga tagagctgga agaacgtttc taaaaagcta
     6361 gtgatttcgt ccatcgtttc tgtatctact gtatggtcac tttctagctg tttgggttgg
     6421 tagtccgatt tttgaggttt cttactcatt gtcggatttt tgataataac catattatct
     6481 gattcatcta tatggacaac cacatgaaag ctagacgata tagtttcctt gtctttgtct
     6541 tcggtaataa cttgttcaac agaaaacagt acctcaaagg tgttctcatt cacttgagaa
     6601 acctgccaca cctgtatatc gttcacacta gaactagtag gaatatcctt acgaatcatt
     6661 tcttcattta atacctgcaa ttcttccgtc aaataatggg tcaatttttc atttcgttta
     6721 tcaatggctt cttgggattg ttgccacgaa aagtagtctt tcgcaaagga tttcacaaaa
     6781 ctttctactt ggttggtatc gacaatctgc tgtttaatga tttctgtttc acggacagta
     6841 tgagtgtcaa tggcagtaaa atttttatag attccaaatc cgacactacc aatcaataaa
     6901 acccataaaa caaaggttaa tttcttatgc gtgccaactt taatcatcgg aacttttctt
     6961 tgcttgctcg gttttttaga tttctttggt ttctccttct tttcttttcg ctcgattttt
     7021 ataatcattt gattcattcc tttcttagtt atgaataaca cgtctagccc caatcagatg
     7081 gtcttgccaa taacgactgc ttaagtcgcc ataaccgatt gggtcgcctg catgataaaa
     7141 gcgatttccc tctaaatata tcgccacatg agtcacatat gtgcctgcat tataggttga
     7201 atgaaagaag ataaggtcgc ctgcttgggc ttcttccata gagatttctt gcgtagcgtc
     7261 atactgatct tgggcgacac gagggagaga aatgccagct ttgtcataca cccattgtat
     7321 taagccacta caatcaaaag aggtggtagg cgacgcccct ccaaagacat agggaaaccc
     7381 ttcgtatttc aaggcttcgt ccataatgac ttgcacggtt tcatcatcaa attctgttgg
     7441 ggaagtgtcg gttaggtatt gtgaaaccaa ctgaacataa aactgatttc catagttata
     7501 tcgccaacca ccattcacag gtatggctat aggattggga tagtctgctt tttgaccacc
     7561 cgacttctct ttagaaaact gttcggctaa ttcataggta tattttttcc catgactcct
     7621 cacataattt aaaaagccac cgccataatt ataagattgg ataacagaat ctatatccac
     7681 tccttgttgt tctgcactgg ttaataattc actgaaatat ttaacccctt gtttgatgga
     7741 ctcttcggta ctcaaactgt tgggcggtaa acccaaagat tcggaacttt gcataacatc
     7801 ttctgctgta ccgcccgatt ccacttgcat aatggctaag atataagaaa catagtcttc
     7861 aatcccatat tccttaccat atttttcaat caacggacgg tgtgccaaca cttctggtga
     7921 aacggtcaca ccgccttgtg gaatagaaga atccttgttg ttttcgccac tgtccgtatc
     7981 atctgcaaag aaaatgacta ggaataataa gagaaaaaag aagagagaga agaccacact
     8041 acaaatcact gttattttta gcttcatgtt tgttgtcctc gcttatccgt tcttttattt
     8101 agcttcctct gattgaattg cttcggtggt gtcttttgtt tttgctcttt tttcaatggt
     8161 cgctgatgat tttcacgttt agtgttttgt gacaaaacag gcgtgacggt ttgctgacgc
     8221 ttgcgaacat gctgctggtt attctgaaca ggagatgatg ggttttcttt tcgtcttttt
     8281 tcttttgctt tatccagctc caaacgcttt tgagcaatgt tctttcgttt ttgctcctgc
     8341 tggttcatac gctgttgttt gcgttgagct ttggtatctg ccatagattg tttaaagtct
     8401 gttacattat gagtgacatt ctcttttcct tgatgaaggg catatttgac atgggttggt
     8461 gtcgcctgta tctgttgttt tgcctgcttt cttttattga ctagacgatt tttcgtgtcc
     8521 aatacatggg ctgtcttttc acctaatcgt tgcccgatac cgccttgttg ctttttcaca
     8581 ttggaaagtg ctgtttcttt ctttggtgta gtccgttcat tttgacgagt atggttagct
     8641 ttattggttt gattctgctt tgttgtcatt gtcttttgct tcgttgaccc tgcttttcca
     8701 cgaaaaatct tactcatact gcgttgaaat cttctggttt gtcgattcat catttgacga
     8761 ggtctgcgaa acacttgtct gcccatgttt tgagagtcgt tgctttgtaa agtgaatagg
     8821 ctcattaaat cgcccagttt aaaataaatc cctgcaaagg tcacaatttg taaaaacata
     8881 atcatgaaaa agggagaact tgctgacagc gaataaagca tagtggaaat actaaaggca
     8941 acggtaataa tcaaggtaat accagccctt gtcataatca cattaaataa cttggtaatg
     9001 gcatgcctgc ccatgccatc aaaactcggc aacatactta ataggaaact gattggcaaa
     9061 aacatagcga aaataataaa tagcacttgc gaaaaaatca tgattcctgt tagaaggaaa
     9121 acaaaaatcg agataccaat attgaataca aacaaaaaga agactgttcc taatctggaa
     9181 atcgtcttcg gtagggttaa ataggtattg tcatgatctt caatttcttc aatcactaca
     9241 ttttctctat cttcaccatt gtttgtatct ggactggtag acaataaact ttccacacgt
     9301 tcttccccta acgcttctac atcagtggag ccatattgaa gcaatagcca tggttgcttt
     9361 acttgaacag agaataagct gtcacgtatc atgtccacac tgtctttccc ttgactatcc
     9421 gtgttaggaa gaacaatttt tgtcccaata ttcagactgg cggtactgac atctttggaa
     9481 aaatcattga ttttcgcaat ataatcgggt gcatacgcaa taaaggaagc cgatagaata
     9541 aacaccacga caaagtttaa aacagctcgt atcactttcg tcgtttctcg cttaaccaat
     9601 cctgtatagg caacatagat tccaacaatg agaataaaaa gaaggaggaa accgacataa
     9661 aaccctgaac tggaaaagcc attcggtgta atgcctgcaa tcgtctgcat gttttcgcca
     9721 attgcttggg atgtttgact aatgaaatct aaggcatagg cttctttcac caaataacct
     9781 gtggcgttcg atagataaag agaaattgtc caaataaaat tagtaatggc atacaatcca
     9841 tacatcaccg attgtcctat gccatcactc caattccatg gaagccagcc ccagctattg
     9901 tccacataat aatcaagctg atattgattc aaaccatact tggagtattc attgcttgta
     9961 tcaatggttt catcaactaa gcctgtcgct tgtactgcat tgcccagcgt gcctaataaa
    10021 agtaaaataa cgcccacaca aaggagcgtt atccaactat agtgaagaaa tttcttttgt
    10081 ttttgtgtca ttcttccacc tcatttcgca caggcggtct ggtatcaaag gcatgaagca
    10141 actcttcaaa aatcgggtga aactgcataa cccctacacg accatagatg tctgaaacta
    10201 aacattgtcc attttctaat tcacgtaagc gtttctgatt gttttcatct tccttgtcca
    10261 caccaaaaaa ttctaaggtc tttttgattt ccactaggtc agtggaacga aaggcaaatt
    10321 tcaagccaat attattcttc atcttttcat ctagtaaatc gtccgcgttt tgagtaacga
    10381 aatagacccc tgcattcatg gcacgtcctg ctcgaaccaa tcgcatagat agagcttttc
    10441 cttgtgccac ttgtaaaaaa ctccaagcct catcaaggtc aacaatttta aaaatggaac
    10501 ggtcagagtg gataaagtct aaagcaaagg tactaatgac aatcagcatt gccacactta
    10561 ataattccat ggtagtgtat tcttcaaaag agctattgga atcgggtaag accaaatctg
    10621 ccacttgaat aatattgagt tgtttttcca agctaatgga ttgggtgaca ctgccatcac
    10681 tgaataacag atgggcaaaa tcataatcga caaagctttc gatatggtca gcaatactat
    10741 tgctgatggg cgtatcttct tttcgcaatt catcaatcac caataacaac cctcgttgtt
    10801 tgctttgagt tacactccga atagctttac gcaggacagg aaacttctcg ctatctcggc
    10861 tggaaatacc tgttaagaac gtgagaatat caatggcaag gctttctgaa tctttcggga
    10921 gttttaaaat cacataaggg tcaagtaacc ctttattgtc gttctcactc gtgagattaa
    10981 caatattgat ttcatgggca atatcaggta aggtttcttt ccattcacca cgttcagctt
    11041 ttgggtcaac aatggttgcc tgtccgccaa agagtactga ataatagacc agcatattgt
    11101 tagagaatga ttttccgcca cctaatgaac caataaaagc ggacgctaat gcatttgtta
    11161 cagagccttt aattccttga cttgcaaggc taggttggag ataaatattt ctgcctgtat
    11221 cgagattgta accgacgtaa ataccagaat tttcaccaat catttgtgta gcaccgaaac
    11281 caagaccagc caaaaaatcc gacgtgacat attgaatata gtcattgata tatcgcttgc
    11341 ttgagggaat aaattcatta tgcaagccta gcatatcgcc aaaaggtcgt actaatttga
    11401 tagacagatc atcataaaaa tcttttactt catcacaacg ttgtttcagc tcgtccaaat
    11461 tctttgctga tacccgtacc acatagctta gtttgtacat agattctttg gtttggtcta
    11521 aattactttc taattcatcg acagattcta aagcgtccac cacattagta ctggtttcgt
    11581 tgtcgctttc ccatgcgtgg ctatctaaat ctttcagttc tttcttttta ttgcgaacgg
    11641 tagataaggc ttttttattg gcgacaattt caacattcat actggtatca acggggaaag
    11701 taaactgctg ttgctggtaa taaaaaattt cactattagg gaaatccaat tcaccaacaa
    11761 tactattaat tgtaaaatac gccacataag tagtggtatc ttcctgttca atccgtaaat
    11821 agcgttgatt ttcttcgatt aaacagcgag tgggtttgag aatatcgtat ttctttacca
    11881 acactttttt atcttcgtat cgtttcggta aatggtagtc gtagtcatca aaagggacac
    11941 ctgtttgccc gtaaagatgt tcaataatat aaccaaaatc atctttctcc aatttacgaa
    12001 tctgaaagcg tctggataat ttactttcta acaactgttc catcttagaa aagcgttcga
    12061 tttccgccat aggcatagaa acaaaatcgc ccattaattt gtgattcaca ccatgaaaga
    12121 attccgcaaa tgctttttga atttctttcc caacattttt tacggatact tcctgttcat
    12181 tgaggtagag tttaaaacca ataaaaaagc gatagtctac ttgattgtca ccaatcatag
    12241 aaattaacgc ttcggtctgt tcatctattt tttcgtaagc tatctctttt aatcgtccag
    12301 tgatttcttt tttcgattgt tcctgtgtgg ctcgaatact tgattcggtt gctagttgca
    12361 gagcatgaat cttgccatca cgatttt
//
LOCUS       DACDMM010000380         5158 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.guided.159, whole genome shotgun sequence.
ACCESSION   DACDMM010000380 DACDMM010000000
VERSION     DACDMM010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.guided.159"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1708
                     /locus_tag="IWD16_002925"
     CDS             <1..1708
                     /locus_tag="IWD16_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9089881.1"
                     /translation="QTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRAY
                     EEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKISTL
                     SGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRYF
                     LDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRAG
                     VQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQT
                     LLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQEN
                     EEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKRR
                     LFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVADK
                     LLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEWE
                     TIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEWV
                     ED"
     gene            1720..2667
                     /locus_tag="IWD16_002926"
     CDS             1720..2667
                     /locus_tag="IWD16_002926"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9089882.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            2687..3214
                     /locus_tag="IWD16_002927"
     CDS             2687..3214
                     /locus_tag="IWD16_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9089883.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3277..3930)
                     /locus_tag="IWD16_002928"
     CDS             complement(3277..3930)
                     /locus_tag="IWD16_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9089884.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4062..4904
                     /locus_tag="IWD16_002929"
     CDS             4062..4904
                     /locus_tag="IWD16_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9089885.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5062..>5158
                     /locus_tag="IWD16_002930"
     CDS             5062..>5158
                     /locus_tag="IWD16_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP9089886.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKA"
BASE COUNT         1839 a          903 c         1080 g         1336 t
ORIGIN      
        1 gcaaacagtg ttttatccaa ctgattaccg tattggttat ctttctcaag cagctgaatt
       61 ttccgatgaa ttaactgttc tccaagcagt ctttcaagga aacagcccct tgatccaaac
      121 tgttcgagca tatgaggaag cattgatcga actaggggaa aacggagaag accctgatgt
      181 gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa gaagatgctt ggactacaga
      241 taccaacgca aagattattt tgcaaaaatt aggaatcagt gaattagata aaaaaatcag
      301 tactttatca ggtggtcaga aaaaacgagt tagtctggcg caagtgttga ttgatgaacc
      361 cgatttacta ttactagacg aaccaacaaa ccatttagat tatgaagcga tcgagtggtt
      421 ggaaaactat ttgaaacaat accgtggcgc actattgatg gttacccacg atcgttattt
      481 tctagataga gtagcaaacc gaatcttcga actttcattt ggaaaattat acgagtataa
      541 aggaaattat gaagcttacg tcttagaaaa agcagaacgt gatcgtgtag aagtagaaca
      601 agaagaaaag cgcaagcggc tttataaaca ggaattagct tggatgaggg ctggcgtaca
      661 ggcacgtggc accaagcagc aagcacggat caaccgtttc gaagatctaa aggagaactt
      721 gtaccaagta aaccaagaag atgatttaga gctgaaccta gcgacacaac gtttagggaa
      781 aaaagtactg gaaatcaaag atggcagtta ccgtataaat gaccaaacac tgctagaaca
      841 tttagattta ttgattcaat ctcgggaaag actaggtatt actggtaaaa atggtgcggg
      901 aaaatcgaca ttgcttaata ttctagctgg cagaattccc ctggatagcg gtacaatgtc
      961 tatcggtgaa acggttcatc ttgcgtatta tacacaagaa aatgaagaaa tggcaccaga
     1021 taaacggatg attgcttact tacaagaagc tgctgaagaa gcaaaaacag cagatggcag
     1081 tcaaatcgga gtagcagaat tacttgaacg atttttattt ccaagattta tgcatgggac
     1141 actgataggc aaactttcag gcggagaaaa acgccggtta tttttgttga aactgctgat
     1201 tcagcaacct aatgttttat tattggatga accgaccaat gatttggaca tagcaacatt
     1261 gacgattttg gaagattatt ttagaagttt tccaggtgct gtgatcactg tttcccatga
     1321 ccgttatttc ttggacaagg tagcagataa gttattagta ttccaaggaa acggaaaaca
     1381 agaactttat tacggaaaca tgtccagtta tttactgaag caaaaagaga cccagcagcc
     1441 tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct gctgaaaaga agaaattatc
     1501 ttacatggaa caaaaagaat gggaaacgat tgaagatgag attgcagaac ttgaagaaaa
     1561 aatctcattg ctccaagaag agatgaatca tcaaggagat aatttcactc gattacaaga
     1621 attgcaaaac gacgtatctg aaacagaagc acaacttgaa gaaaaaatgg cacggtggga
     1681 atacttgagt gaatgggtag aagattagga ggaaaaagga tggaacaagc gtatcttgac
     1741 ttaggaagaa aattgttgga agaagggcat cgtaaagaag acagaacagg cacaggcaca
     1801 ttaagcttat tcggatacca aatgcggttt gatctgcaaa aagggtttcc tttgttaaca
     1861 acgaaacgag taccttttgg attgatcaaa agcgaattgc tttggttttt aaaaggagat
     1921 acgaatatcc gctatctatt acaaaataaa aaccatattt gggatgaatg ggcatttgaa
     1981 cgctacatca aaagtccaga ctataatgga ccagatatga ctgattttgg ccatagatgt
     2041 ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga gcaagctttt ttgcgagaag
     2101 atcctaaccg atgattcctt tgctgaaaca tacggcgatc ttggacacat ctacggctat
     2161 caatggcgtc actgggaaac aaaagacggt ggctttatcg atcaaatcaa acaagtgatc
     2221 gaagcaatca aaaagactcc tgattctaga agattgatcg tttctgcatg gaatccggaa
     2281 gatgttccta gcatggcttt gcctccttgt catacgatgt ttcaatttta tgttcaagag
     2341 ggacggctaa gctgccaatt gtatcaacgc agtggggatg tgtttctagg cgttccgttc
     2401 aacattgcaa gttatgcgtt gctgactcac ctaattgcac atgaaacagg tttagcggtt
     2461 ggcgaatttg ttcacacatt aggagatgct catctttata tcaatcatat cgaacaaatg
     2521 aaagaacaat tgagccgaga aatacgcgca tttccgactt tagttttgaa tcaggaaaag
     2581 cagtcagtat ttgattttga gatggaagat atccaattag aaggatatga cccacatccg
     2641 gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat gagaaaatgt tagctgcaat
     2701 ttgggcacag gatgaaaacg gattgattgg aaaagaagac cgtcttcctt ggcgtttgcc
     2761 aaatgatctg aaattcttca aacaaatgac agaagcaaat acactagtga tgggacgaaa
     2821 aacattcgaa gggatgggta gccgtccgtt gccgaatcgt cagacgattg ttttgacgag
     2881 agattcttcc tataaagcag aaggcgtgca tgtcatgcat agtgtggaag acgtgcttgc
     2941 tctggaaaaa gaaacagacg gtatcttttt catcgctgga gggtcagctg tttatcaaga
     3001 atttctgcca ttttgtacga tattataccg tacagtcatc catcatgtat tcgatggaga
     3061 tgcttatttt ccacctgttg actggtctga ttggtcactg atcaatacta gtcaaggaga
     3121 aatggatgaa aaaaatcgtt atccgcatca atttgaaacg tatcagcgta atgagaatcc
     3181 tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc tatggataga ctagctgtta
     3241 tttagagctg tcccatccat agctttattt tttttattaa gtaaagaata aaacagagaa
     3301 gtacataaaa cctgcaccta acataacaaa caaatgccaa acaacatgca tatagcggat
     3361 agaattcaag ctataaaaga atgcgcctat cgtgtaagaa agcccgcctg ctaccatcaa
     3421 aataacacca gtcattccaa gagagtgata aagaggaaca gcagccacga cacagagcca
     3481 ccccataaaa atatagatga tcgtagatac ttttgatact ctctcccgtt tatgcaacgt
     3541 caacgcttta tatataatgc caagaatcgc aaacaaccag attaatacaa ataaagtcca
     3601 acctagccat cctcggatac tcagcaaaca aaaaggagta taacttcctg caatcaaaag
     3661 aaaaatcgaa ctatgatcga atacttggaa cacctttttt gcttttgtaa aaatcaatga
     3721 atgaaacaaa gtagacgtta aaaataaaag aatcatcatc gatccataga tagcataaga
     3781 gacaatatgg agggaggaat gcaagtgggc accttttact aaaagaacca ctagtcccgc
     3841 gatactcaat ccagtcccaa ttccatgggt gactgcatta aacacttcat ttaatatttc
     3901 atacgttcga ttaaatttcg ttttttccat gaaaacggct cctttactag gtgtaattaa
     3961 tgaaaaatca ttatttgtct gttatactaa aaatagaaat tgtcttacat gtagtttaac
     4021 atgaataagg catcttgtta aagaaagaga gagagaaaat aatgacaaat gtaaaaatcg
     4081 taacagattc ttcatgtacg atggaaaaaa atgtacgcga tgctttaggc attcgtgtca
     4141 tgccgctatc catcatgatc gacggaacag tatatcccga tgatgaccaa ctagacggcg
     4201 aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc caaaacaagc caaccaccaa
     4261 ttggagaatt catctcgatg tatgatgagc taggagaaga cggcagtgaa gtgatttcga
     4321 tccatatgac aaaaggcttg agtggaacag tagaagcagc aagacaagcc agtaatctaa
     4381 caaaaacgaa agtaacggtt atcgacagtg ataccactga ccaaggatta tcttttcagg
     4441 taattcgagc agctgaatta gcaaaagaag gtaaaacagt agagaaaatc cttgaagaag
     4501 tcaaaaaagt acaagacaat acaaaattat acatcggaat atccacacta gataacttgg
     4561 taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc tagtgttttg aacatacgcg
     4621 tggtgatgaa tttcgcccat tctgaattaa tccctgtaac taaggggcga ggaaagaaaa
     4681 cgttcgataa atggtttaat aatttgaaaa atgaactgaa agaattgcca aatgtgcgac
     4741 aaatcggtat ttcacatgcg gatgcaaaag aattggcgca agaattcaaa gagggtttgc
     4801 aagaattatt cccggacatg catatccctg tactgcatac gaatccggtt atcgctacgc
     4861 atactggtaa agacgcgttt gcaatcatgt attatgtaga ttagtcgaaa agaggataag
     4921 ccctcagcct gaaaggcttg cagcttattc tctttttctt tataaaatta ggaaaataat
     4981 tgttttcttt tttcataaag actacctttt tgataaaatt tttgataaca ttagaaccaa
     5041 tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac atttgatcct cgttttgttc
     5101 ccattattaa ttgcgatcat catctatgca ggtttagctg cttctatacc gaaagctg
//
LOCUS       DACDMM010000381         4934 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.guided.166, whole genome shotgun sequence.
ACCESSION   DACDMM010000381 DACDMM010000000
VERSION     DACDMM010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.guided.166"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            83..1471
                     /locus_tag="IWD16_002931"
     CDS             83..1471
                     /locus_tag="IWD16_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9089887.1"
                     /translation="MEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKG
                     FRNTYFKQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEI
                     EAYLSELNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKP
                     RFIDEFVLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLK
                     YYQWKMKKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELN
                     KLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAH
                     IFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLP
                     VVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVK
                     ERLEQIIGSLTG"
     gene            1478..1855
                     /locus_tag="IWD16_002932"
     CDS             1478..1855
                     /locus_tag="IWD16_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9089888.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            2009..2791
                     /locus_tag="IWD16_002933"
     CDS             2009..2791
                     /locus_tag="IWD16_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP9089889.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(2917..3648)
                     /gene="erm(A)"
                     /locus_tag="IWD16_002934"
     CDS             complement(2917..3648)
                     /gene="erm(A)"
                     /locus_tag="IWD16_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP9089890.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(3706..3793)
                     /locus_tag="IWD16_002935"
                     /pseudo
     CDS             complement(3706..3793)
                     /locus_tag="IWD16_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            4157..4819
                     /locus_tag="IWD16_002936"
     CDS             4157..4819
                     /locus_tag="IWD16_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9089891.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         1739 a          708 c          935 g         1552 t
ORIGIN      
        1 tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta attcctacta
       61 tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg atggagagga
      121 gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata aggttaaccc
      181 aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt ttaaacaact
      241 tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg taagtgatat
      301 tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac aagttcagag
      361 agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa acatgatggg
      421 aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat ttagtaccct
      481 tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg aggactatcc
      541 gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc aattgaacag
      601 tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc aagaatgtgg
      661 aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg acaaagatgg
      721 agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag ttccaatatc
      781 taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg aatttccaga
      841 tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa catttagagg
      901 tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg agatttatag
      961 attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca acgggatgcc
     1021 ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa gcagatacgc
     1081 tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa aactggttac
     1141 caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg tagagcttca
     1201 atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta gattgccagt
     1261 agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact tctgtaccag
     1321 taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt tattagccat
     1381 agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta aagagcgttt
     1441 agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag ttagaaatac
     1501 aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa aagtagacaa
     1561 agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa tagcaaagga
     1621 agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa gaatcgaatc
     1681 ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa gttctcgttc
     1741 ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa aagaaaataa
     1801 aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag aataattatt
     1861 aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct ttattgaaca
     1921 gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat aaactttata
     1981 tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa aaataccaaa
     2041 tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa tagttggagt
     2101 atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg tagatgttct
     2161 agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag aaagactaat
     2221 gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag ttacggttat
     2281 aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta tatacggtga
     2341 gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg atcctgattt
     2401 ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc ctgattcttc
     2461 aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg attctttgcc
     2521 agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc tagctcgaat
     2581 gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag aatgggctat
     2641 acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag gctatcgggg
     2701 agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg ttaagtatat
     2761 gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa caggtgctta
     2821 cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga ataaagcgtc
     2881 ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt tgtaactatt
     2941 gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat ttgcatgctt
     3001 caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt ttacccactt
     3061 ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt gatgtcgttc
     3121 aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg gtggtacttt
     3181 tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc gttgcagatt
     3241 ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag cctgactttc
     3301 aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac catatatctt
     3361 atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca cttttatatt
     3421 ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa tttctatagc
     3481 agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc ctgatccgat
     3541 ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt ttacatgctt
     3601 tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta taaccttctc
     3661 cttactggtt ataatgaact gacattaagc gttcattata accaattaat tttgatttgg
     3721 ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt tatctccttt
     3781 ccattttttt cattatgaat gagataaagt aatttctact actgcgatac tggtgcacat
     3841 aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt tagaataaac
     3901 ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa ataatatgtt
     3961 ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc gataaaggac
     4021 agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc taaagggaag
     4081 ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt cgataaagta
     4141 ggaatggggg atctttttga atacaataga atcacagttt gataaggttg cagaagatta
     4201 cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata tgtctccaaa
     4261 gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga aattagcaag
     4321 ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc ttgccaaatc
     4381 taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac ttaattttaa
     4441 tgagaagttt gattttattg taagccgaac aacctttcat catttggatg atatagccag
     4501 cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta ttcttgataa
     4561 tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa tccaagagtt
     4621 tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata atcacaacac
     4681 atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac aaaattatta
     4741 cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt gggcaatggg
     4801 ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt gtgattgttt
     4861 tcagagctat cgggtgcttt agtataaata acaatgaata aaaaatcagc ctgtatctta
     4921 tgttactatt aagt
//
LOCUS       DACDMM010000382         3056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.guided.222, whole genome shotgun sequence.
ACCESSION   DACDMM010000382 DACDMM010000000
VERSION     DACDMM010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.guided.222"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(40..1026)
                     /locus_tag="IWD16_002937"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="IWD16_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="IWD16_002938"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="IWD16_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9089892.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="IWD16_002939"
     CDS             1851..3005
                     /locus_tag="IWD16_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9089893.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          939 a          602 c          630 g          885 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttct
//
LOCUS       DACDMM010000383         2967 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.guided.224, whole genome shotgun sequence.
ACCESSION   DACDMM010000383 DACDMM010000000
VERSION     DACDMM010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.guided.224"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..575
                     /locus_tag="IWD16_002940"
     CDS             <1..575
                     /locus_tag="IWD16_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9089894.1"
                     /translation="IIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLDKQAGKIAQQ
                     IKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKIEQADVIITG
                     EGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPIG
                     LEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            833..2335
                     /locus_tag="IWD16_002941"
     CDS             833..2335
                     /locus_tag="IWD16_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9089895.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2402..2929
                     /locus_tag="IWD16_002942"
     CDS             2402..2929
                     /locus_tag="IWD16_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9089896.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1017 a          516 c          666 g          768 t
ORIGIN      
        1 tgattatcgc tgcagatgtt accaatccat atacaggtca acaaggagct gcttggcaat
       61 ttggaagaca aaaaggagga atagatacga cgctagcttt cttagataaa caagcaggaa
      121 agattgctca gcaaataaaa gatttctggg aggtggattt agatgatatt ccaggaagtg
      181 gagcagcagg cggtattggt ggagcccttt ttttgttggg agcgaagatg gtttctggtt
      241 tcgatttagt cagccaaatc actggattgg aaaaaaagat cgaacaagca gatgtcatca
      301 ttacaggaga agggcggatc gatcagcaaa cgatccacgg aaaagttcca tacggtgtag
      361 cagtgcttgc aaaaaaatat cgaaaaccaa taatcgctat ttgcggaagt agagaccaag
      421 agctagaagg actttctgat tttctcccaa ttgtcttaag tattcatttg ggtccaatag
      481 gtttagaaga agcaatggaa cacaggcaaa cattagaaaa aacaaaaatc ttaggacaga
      541 cacttagcag atttttcacc atcctcccga agtagttgac aaaatcattt tctttatgct
      601 atcgtaattt caaatatagg acaaagtgag ggatccgtgc attgaaaaat taagtcgatt
      661 gatgaaagct gcattcagac tgtaccttgt ggagacaagg aagcagtgtg cagattttcg
      721 ggattggctt ggttttttcg atgtatgggt ctttttgtgt gtatccaacc cttgcatcct
      781 ctttagccag ttctgaaaat aggactggtc tttttttacg aggtgatgga aaatgtctaa
      841 aatcgaaata aaaaatctga cattcggcta cgacagccaa ggcacattat tatttgaaca
      901 agcaaatcta aattttgaca cacaatggaa actaggactt atcggacgaa acggtcgagg
      961 aaagacaact ttactgaata ttctacaaaa caaactacct tatcaagggc aagtaatcca
     1021 tcagcaagaa tttgcctatt tcccgcaaca gacaaaagat aaagaacgtt taacctatta
     1081 cgtgttaaat gatattacgg attttgagat atgggaaatc gaaagagagc tccaattgat
     1141 gcaaacagat cctgaaatct tatggagaga attcagcaca ctatcggggg gagagaagac
     1201 aaaagtccta ctggcacttt tatttgtgga tgacactcat ttcccgttaa tcgatgaacc
     1261 aacgaatcat ttggatatct ctggtagaaa acaagtagcg gcttatttga aaaagaaaaa
     1321 acaaggcttc atcgtggtca gccatgaccg gggatttatc gatgaagtag tggaccatgt
     1381 tttagcaatc gaaaaaagtc aactggaact ttatcaaggg aatttctcta tctatgaaga
     1441 acagaaaaaa cttcgtgatg aatttgaaat ggctcaaaat gaaaaattga aaaaagaagt
     1501 cagtaggcta aagaaaacag cagctgaaaa agccgaatgg tctcgttccc gagaaggaga
     1561 taaaacaaag aaacaagtcg gattcatcga tactgaatct agacgagtga ataaaggagc
     1621 agtgggtgct gatgctgcac ggacgatgaa acgatccaaa gcaatcgtga atcggatgga
     1681 gacccagatc agcgagaaag aaaaactatt aaaagatatc gaatatatcg attctttgac
     1741 gatgaatagc caagcgtctc accataagcg acttttaagc gtagaagatc ttcaattagg
     1801 gtatgaaaat ctgttattcg agccaattca ttttacaatc gagcctcatc agcgggtggc
     1861 gatttcaggt cctaacggtg caggaaagtc atccattatc cattatcttc tgggggcatt
     1921 caacggcaag gttataggag aaaaatacca gccaaaacat ctgagcatta gttatgcaag
     1981 ccaaaattat gaagacaatc gaggaacgtt ggcggaattt gcagagaaaa accaagtaga
     2041 ctaccaagca tttttgaaca acctccgaaa gcttgggatg gaaagagatg tttttcataa
     2101 caagatcgag cagatgagta tgggccaacg gaaaaaagtg gaattggcta aatctttatc
     2161 acagccagct gaactatata catgggatga accattgaat tatttggatg tcttcaatca
     2221 agaacaatta gaacaactga tcttgaacgt gaaacctgcc atgttactag tggaacatga
     2281 tcaaaccttc ctggataaag tatctactga gattatttct cttgagagaa tctaaccctt
     2341 gtaatctcaa gaacaacaag gtatggttaa ggggaacttt gccaacgaag gagagaatca
     2401 catgctttat gtaacgagac acggagaaac aacttggaac gcacaaggat tagtttgcgg
     2461 acgggcagat gtgccgctga ctgaaaaagg acagatgcag gcacagaaac tagcagaaaa
     2521 ggtagtagat ttgcctgttc caatcacgaa aatcatccat tcccctcttc aacgagcaag
     2581 agatacagca caagcagttg ctgatcgtct cagcttgccg ctgaccgttg atgaacgatt
     2641 ggtagaaatg gattttgggg attatgatgg actgccaagc aaagatgaga actttcaaaa
     2701 agcacgttta gcgttcgctg tacgattccc aaatggggag tcagtcttag atgtgtatgc
     2761 acggatcgtt cctttattga aagaatgcat cgaagacgaa gaaaatgtct atctgttagt
     2821 ttgccataat gccttgataa gagtgatcaa tgcttatttt catccgatgc ctaatgaggg
     2881 gttctttaca ttcatggtag ataatacaga attggtttct tatgagtaat tatgtttttg
     2941 tgttatttaa ataaaatatt tcaaggc
//
LOCUS       DACDMM010000384         2556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.guided.230, whole genome shotgun sequence.
ACCESSION   DACDMM010000384 DACDMM010000000
VERSION     DACDMM010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.guided.230"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            163..1131
                     /locus_tag="IWD16_002943"
     CDS             163..1131
                     /locus_tag="IWD16_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP9089897.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1124..2155
                     /gene="vanA"
                     /locus_tag="IWD16_002944"
     CDS             1124..2155
                     /gene="vanA"
                     /locus_tag="IWD16_002944"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP9089898.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2161..2526
                     /locus_tag="IWD16_002945"
                     /pseudo
     CDS             2161..2526
                     /locus_tag="IWD16_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
BASE COUNT          758 a          489 c          632 g          677 t
ORIGIN      
        1 aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga cttaataccg
       61 taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca ttggcatccc
      121 cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa catcggcatt
      181 actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc gcctcgcttt
      241 ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa atccgcgcct
      301 ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat tcttcttgcg
      361 ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa tcatatagat
      421 acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc gccggatagc
      481 gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa atcgattgtg
      541 cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt actcagcgac
      601 atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga gcggctgcga
      661 ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt aaactatgta
      721 ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc gctcaatacg
      781 gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg agcatttctt
      841 atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc attagaaaac
      901 gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt tttctactct
      961 gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag aatgcctaac
     1021 gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga taccgttgaa
     1081 aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata gaataaaagt
     1141 tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat ctgcaataga
     1201 gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa ttacgaaatc
     1261 tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca attgctattc
     1321 agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga accatgaata
     1381 tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag gtgaagatgg
     1441 atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg atattcaaag
     1501 ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg ctgggatagc
     1561 tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta cgtttaccta
     1621 tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa aagtcaatag
     1681 cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca aaatcttaat
     1741 tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca gtgccgcgtt
     1801 agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta ttcatcagga
     1861 agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag acctttcagc
     1921 agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc tcggctgtag
     1981 aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac tgaacgaagt
     2041 caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg ccgctgcagg
     2101 tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg ggtgataagc
     2161 atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg ggacgctaaa
     2221 tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt aaatcgcatt
     2281 gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc tgctacccaa
     2341 gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa ctgttttatg
     2401 caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc caatattgac
     2461 cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag ccgcggcggt
     2521 tctgttgcaa agttttaaat aaagaataaa atccct
//
LOCUS       DACDMM010000385         1557 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.guided.280, whole genome shotgun sequence.
ACCESSION   DACDMM010000385 DACDMM010000000
VERSION     DACDMM010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.guided.280"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            16..1494
                     /gene="msr"
                     /locus_tag="IWD16_002946"
     CDS             16..1494
                     /gene="msr"
                     /locus_tag="IWD16_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9089899.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
BASE COUNT          557 a          269 c          331 g          400 t
ORIGIN      
        1 aggagtgtaa aacatatgga aaatttagca gtaaatataa caaatctaca agtcagcttt
       61 ggcaaccagc tagaactatc tattgattct cttcgtgtct atcagcaaga tcgtataggg
      121 atcataggag aaaatggtgt cggtaaatcg actttgctca aactaatagc cggtgaactt
      181 tttcccgatc atgggaaaat ccaaacagag atcaccttca actacctgcc tcaattaacc
      241 tatctcgctg aagcaaagga cctaaatttg gaattagcca gtcatttcca gttaagactg
      301 gaagaaactt cagagcggaa atggagcgga ggggaagaac gaaagatcga gttgatacgt
      361 cttctttctt cttatgaaca agggatgctt ctagacgagc caacaaccca tctagataga
      421 aaaagtattg atcgactgat tgaagagctt cgttattatt atggtacgct ggtttttgtt
      481 agtcatgatc gctattttct agatgagcta gcatcgaaaa tctgggaagt aaaagacgga
      541 gaaatccgag agttttcggg gaattatagt gcctatctca ctcaaaagga attggagaaa
      601 aagactcagc tacgagaagc agagtcgatc atgaaagaga aaaaacgatt ggaaaaatcg
      661 atccaagaaa agaaaaaaca agcggaaaag ttggaaaaag tgtccagtaa aaagaaaaag
      721 caacaaatca gactggatcg gttgtcttcc tctaaacaaa aagacagtgt acaaaaagcc
      781 atccaaaaga atgcgaaaac attagagaga agactccaaa aaataggaga aacaaccaag
      841 ccgcagcaga tgaaacaaat ccgttttcca gtaccaaaat ctcttgaact ccacagccgc
      901 tatcccatca tgggacaaaa tgtccaattg gaaagaagtg ggagaacatt actggtaaat
      961 ggtgattttc agttttcttt aggtaaaaaa atcgcgattg tcggtgaaaa tggttcaggt
     1021 aagacaacct tattagaaca tatccgcaaa caaggagaag gaatccttct ctctccgaaa
     1081 gtaagctttc aagtatatca gcaaaagggt tatcaaatga catctgaaga atccatcatt
     1141 cgttttgtca tgagacaaac agagttttca gaatcgcttg tccgtagttt gctgaatcac
     1201 ttagggtttg ctcaggaaac tttgacgaaa ccgttatgta cattaagtgg gggagaagcg
     1261 acccgtctga cgattgcttt gctttttact aagccaagta atgtgttgct gttagatgaa
     1321 ccgactaatt ttattgatat ggcaacgatc gaagctttag agaagctaat gcaaatatat
     1381 ccgggaacga ttttgtttac ttctcatgat tcttactttg tcgagcgtac ggctgatgaa
     1441 gtttatgaaa taaaagggca gaaaataaaa aaagtactta cgagaaattt ttaaatgtgc
     1501 tacgatgatt ttcgtgacaa atttaaagga gtggtacaga ttgcctacaa ataaaaa
//
LOCUS       DACDMM010000386         1395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.guided.288, whole genome shotgun sequence.
ACCESSION   DACDMM010000386 DACDMM010000000
VERSION     DACDMM010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.guided.288"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1350
                     /locus_tag="IWD16_002947"
     CDS             <1..1350
                     /locus_tag="IWD16_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAP9089900.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLN
                     TNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFL
                     RQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVF
                     EGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFG
                     EDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          585 a          111 c          237 g          462 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      541 ggttatgata gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt
      601 actaataaga aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat
      661 ttagaaacta atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct
      721 atactaggtt ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg
      781 tcagaagaag aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac
      841 ggtttagatt atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa
      901 gagtatatat tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat
      961 atagaaagtt ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta
     1021 tgccataatg attttagttg taatcatcta ttgttagatg gcaataatag attaactgga
     1081 ataattgatt ttggagattc tggaattata gatgaatatt gtgattttat atacttactt
     1141 gaagatagtg aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga
     1201 aatatagata ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt
     1261 gaaactattg tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa
     1321 gaaatttata aaaggactta taaagattga ttatataata tatgaaaagc tattataaaa
     1381 gacggttctg ttgca
//
LOCUS       DACDMM010000387         1359 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.guided.295, whole genome shotgun sequence.
ACCESSION   DACDMM010000387 DACDMM010000000
VERSION     DACDMM010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.guided.295"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IWD16_002948"
                     /pseudo
     CDS             262..373
                     /locus_tag="IWD16_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IWD16_002949"
     CDS             394..477
                     /locus_tag="IWD16_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9089901.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IWD16_002950"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IWD16_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9089902.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          489 a          204 c          226 g          440 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgta
//
LOCUS       DACDMM010000388         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.guided.297, whole genome shotgun sequence.
ACCESSION   DACDMM010000388 DACDMM010000000
VERSION     DACDMM010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.guided.297"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IWD16_002951"
                     /pseudo
     CDS             46..297
                     /locus_tag="IWD16_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IWD16_002952"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IWD16_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9089903.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DACDMM010000389         1196 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.guided.309, whole genome shotgun sequence.
ACCESSION   DACDMM010000389 DACDMM010000000
VERSION     DACDMM010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.guided.309"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            259..>1196
                     /gene="vanY"
                     /locus_tag="IWD16_002953"
     CDS             259..>1196
                     /gene="vanY"
                     /locus_tag="IWD16_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9089904.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          428 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaataa
//
LOCUS       DACDMM010000390         1031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20309_2015
            SAMN12639542-rid9891363.guided.323, whole genome shotgun sequence.
ACCESSION   DACDMM010000390 DACDMM010000000
VERSION     DACDMM010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639542
            Sequence Read Archive: SRR10030949
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742779.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742779.1
            Genome Coverage       :: 73x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/24/2020 00:51:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 2,954
            CDSs (total)                      :: 2,890
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 64
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 33 of 74
            Pseudo Genes (incomplete)         :: 34 of 74
            Pseudo Genes (internal stop)      :: 18 of 74
            Pseudo Genes (multiple problems)  :: 10 of 74
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639542-rid9891363.guided.323"
                     /isolate="E20309_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            334..882
                     /gene="aac(6')"
                     /locus_tag="IWD16_002954"
     CDS             334..882
                     /gene="aac(6')"
                     /locus_tag="IWD16_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9089905.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          361 a          151 c          207 g          312 t
ORIGIN      
        1 cgattccctt tgcttttttg taattatcta aaaattctaa aaattaagat tattatatga
       61 aaacaggttt tatttgtcaa ttcataattg gtttttttta aagcaaaaga taatctatat
      121 catgatgaac aaacagagtt ttttcctcca attgatggct ttcctgatct ttcctatttt
      181 gaataaagtg tttctgtata atttttttat tttataagta agataaataa tgaaagaata
      241 gagatactga ttggtagcta aagatttttg agcgagaaca tttcgttttt tcaaaaggac
      301 tgttagaata agcaagaaca agggggaaga cgtatgataa tcagtgaatt tgaccgtaat
      361 aatccagtat tgaaagatca gctttctgat ttactgagac tgacttggcc ggaagaatat
      421 ggagacagct cggcagaaga agtagaagaa atgatgaatc cagaacgaat cgcggtagca
      481 gcggtagacc aagatgagtt agtaggattt attggtgcaa tccctcaata cggtatcaca
      541 ggttgggaat tgcatccatt agttgtagaa agctcccgac gaaagaacca aataggtact
      601 cgattagtca attacttaga aaaagaagta gcttccagag gaggaatcac gatttattta
      661 ggtacggatg atttagacca tggaacaacg ttaagtcaaa cggacctgta tgagcataca
      721 tttgataaag tggcttccat ccagaacctt cgtgaacatc cgtatgaatt ctatgaaaaa
      781 ttaggttata aaatcgtagg tgtcttacca aatgcaaatg gctgggacaa accggatatt
      841 tggatggcaa aaacgattat tcctcgacca aattctcaat aataaaaaga ggttgtgaca
      901 gaagtaatca gcttcaagaa ataagaagga atttacgaaa attacttctt gaatttttgt
      961 gaaattccaa cttatttccg aagaagctac ttttggaaca ccgtgtacca gaggttgtga
     1021 aaaagtcgct t
//
